LOCUS       DASRNX010000001         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_584091,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000001 DASRNX010000000
VERSION     DASRNX010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584091"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1513)
                     /gene="thiI"
                     /locus_tag="VFD89_00005"
     CDS             complement(347..1513)
                     /gene="thiI"
                     /locus_tag="VFD89_00005"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HZJ56610.1"
                     /translation="MEKLILVRYGEIYLKGQNRPFFERALIDNINRTLENNDRAKVFK
                     SQGRIYIEGVPVENAVLGDLSKVFGIIGINPAWKTQKDIDSIKTMLRSIVEDALTKTA
                     EENPTFKVESRRADKTFPMGSMDLNREMGAFILQSFPALKVDVHNPDIRINLEIREYA
                     YGYHENVVGAGGMPVGTNGKATLLLSGGIDSPVAGWMIAKRGVQLTAVHYHSFPYTSD
                     KSKEKVIDLCRILSQYCGPMRLYVVPFTEIQQELYEKCPDGLLTILMRRYMMKIAEEV
                     AVKTGGQALITGESIGQVASQTMEGLASTDDAVSLPVFRPLIGLDKTEIVEVAKRINS
                     YETSILPYEDCCTVFVPRHPTTRPNLNKVRNAEALVDGPALIERAMEGIEIYDL"
     gene            complement(1519..2685)
                     /locus_tag="VFD89_00010"
     CDS             complement(1519..2685)
                     /locus_tag="VFD89_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZJ56611.1"
                     /translation="MMKRQIYLDNAATTPLLPEVMDEITRCLAEDFGNPSSLHRLGLQ
                     SERRIKDSRNSIARLMNVPTEDVIFTSGGTESNNLAILGATRARNRYGKHCITTQIEH
                     SSILNTFGYLEGEGWDISYLPVDNHGILDLDKLKDAIKPDTVLVSIGYVNSEIGSIQP
                     IREIGMFLKSNHPDIIFHSDAVQAFGRLNLKPLDWGLDLMSASGHKVHGPKGVGILYI
                     RKGTPLATLQWGGGQEMGIRSGTENLSGIAGFGKAAELLHQFMDRDDNTIAGMKKALA
                     EGIAKAVPEAVLNGPMPDRGAPHILNISIPGIRGETLLHVLESHGVYVSTGSACSSRR
                     TKISHVLYAIGVTGPVAEGAIRLSLSYMSDPDELSSVPDILRESVDQVKKFTRR"
BASE COUNT          705 a          758 c          557 g          842 t
ORIGIN      
        1 ttctggtacc ccctatgacc atggtaccac ccccactatc attatcgatt tttgtccata
       61 tatcttccat ataccttatg cccggctcca tagatagaat ttagacggca taaaacattt
      121 tctgttcaca tacgtgaaaa atcatagcta atgcatgttt ctgagcaaca tttatagaaa
      181 gcccgccatc taatatgatt gttatccccg ggattaattc cggcgctttc tgaaatgaaa
      241 tcaatttatt atattcatga tagtagaaga ttagacctag ctcccaaatt accctgccca
      301 tcaatgaacc caaggttcgc ttttcgttac gatagctaac ccatgcctaa agatcgtata
      361 tctctatccc ctccatggct cgctcaatca gtgcgggtcc gtctaccaga gcttctgcat
      421 tcctaacctt attcaggttt ggcctggtag taggatgcct gggaacaaac actgtacagc
      481 aatcctcata gggcagtata gaagtctcat aggagttgat tctttttgct acctcaacta
      541 tctcggtttt atcgagaccg ataagcggcc tgaataccgg caaggatacc gcatcatctg
      601 tagacgccaa tccctccatg gtctggctgg ctacctgccc aatgctctcg cccgtaataa
      661 gggcctgacc cccggtttta acggcaactt cctcggcgat cttcatcatg tatctcctca
      721 tcaatatggt gagcagcccg tcggggcatt tctcgtagag ctcctgctgt atctctgtaa
      781 agggcacaac atacagcctc atgggaccgc aatattggga taggattctg catagatcga
      841 taaccttctc tttggattta tcgctggtat atgggaagct atgatagtga acagccgtaa
      901 gctggacccc tcgctttgct atcatccacc cggcaaccgg gctgtctatt ccgcctgaaa
      961 ggagcaatgt tgcctttccg ttggtaccca ccggcatgcc cccagcgcct actacgttct
     1021 catgatagcc ataagcgtac tcccttatct ctagatttat tctgatatcg gggttatgca
     1081 catctacctt tagggcggga aaactctgta gaataaaagc ccccatctcc ctatttaagt
     1141 ccatagaccc cataggaaag gtcttatcag cacgtcgtga ttcaacttta aaagtagggt
     1201 tctcctctgc cgtctttgtc agagcatctt caacaatgct tctcagcatg gtcttaatgg
     1261 agtcaatatc cttctgggtc ttccatgccg gatttatccc tattattccg aatacctttg
     1321 ataagtcgcc gagaacggca ttttctacgg ggaccccctc tatatagatc ctgccctggc
     1381 tcttaaaaac cttggcccta tcattatttt caagggtcct gttgatattg tctatcaagg
     1441 ccctttcaaa aaaaggccga ttctgacctt ttaagtatat ttctccgtat cttactagga
     1501 ttaatttttc catagatgct acctcctggt aaattttttt acctgatcga cagattctct
     1561 cagtatgtct ggcaccgatg ataattcgtc cgggtcgctc atataggaaa ggctcaatcg
     1621 tatcgcgcct tccgcaaccg ggcccgtaac cccaattgca tataaaacat gacttatttt
     1681 ggtccttcgg gaagaacaag cggatccggt gcttacatac accccatggg actctaacac
     1741 atggagcaag gtttctcctc tgatgccggg tatactgatg tttagaatat ggggggcccc
     1801 tctatccggc atgggcccat taagaaccgc ttccgggacc gctttcgcta tgccctctgc
     1861 cagcgccttc ttcatcccgg ctattgtatt gtcatccctg tccataaact gatgaagcaa
     1921 ttcggcggct tttccaaacc ccgcgatgcc ggaaaggttt tctgtaccgc tgcgtatccc
     1981 catttcctga cctccccccc attgaagggt agcgagggga gttccttttc ttatgtataa
     2041 gatccccaca ccctttggcc catgaacctt atgtccactg gcgctcatta aatcaagccc
     2101 ccaatctaac ggcttcaggt ttagcctacc aaaggcttga actgcatcgc tatgaaatat
     2161 tatatcggga tgattggatt ttagaaacat gcctatctcc cttatgggct gaatggaccc
     2221 aatctcactg ttcacatatc cgatactgac cagaacggta tccggtttaa tggcgtcttt
     2281 gagtttgtcc agatccaaga ttccatgatt atccacgggt aaatagctta tatcccagcc
     2341 ctcgccttca agataaccaa aggtattcaa tatagaagaa tgctctattt gggtggttat
     2401 acaatgcttc ccatatctgt ttcttgccct ggtcgctccc agtatggcta ggttattcga
     2461 ttcagttccc ccggaggtaa agatcacatc ctcagtcggc acattcataa gccgtgctat
     2521 ggaatttcgg gaatccttaa ttctacgctc ggattggagc cccagccggt gcagtgacga
     2581 cggattacca aaatcctcag ccaaacacct ggttatttca tccattacct caggtaataa
     2641 tggcgtagta gccgcattat ctaaatagat ctgacgcttc atcatagcgc tccttcatca
     2701 tttttgccaa ggggatcccc cctaaacctt accctagttt atccttttct ctatcatatt
     2761 acaagatact attctctgga ttccgatacg acctagttac agatggtcta tatgttttac
     2821 gataaagatt ctacattaag atacaggtat cgtaaaacat tt
//
LOCUS       DASRNX010000002         6142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_318807,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000002 DASRNX010000000
VERSION     DASRNX010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318807"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..509)
                     /locus_tag="VFD89_00015"
     CDS             complement(21..509)
                     /locus_tag="VFD89_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HZJ56612.1"
                     /translation="MRFVVRQRIFSFGDSFTIKDEHGNDHFVVKGRVFTLGNKLRIYD
                     MAGQELYYIEQKILRFLPEYTIYQSGRPMAKVKKEFTFFKPRFNINSTLGNFTIQGNF
                     LGMNFSILKNGEQVAEVSKRWFSFSDTYGVDIKEGEDYGFLLALVIVIDQVLHDNNHN
                     NN"
     gene            902..2176
                     /locus_tag="VFD89_00020"
     CDS             902..2176
                     /locus_tag="VFD89_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZJ56613.1"
                     /translation="MKRFMHAKDLKMIPVTQRFPGDKVDNIEKTTREQIQKTGVRFKK
                     GSRIAITAGSRGIANIATITKAAVDEVKSQGALPFIIPAMGSHGGATPEGQREVLEHY
                     GVTEEAMGAPIISSMDVVSIPNEGNDFRLYMSKDAYESDGVIVINRIKPHTDFHGQVE
                     SGLMKMCVIGLGKHAQALEMHRLGVYGLRELVPKAARKILATGKIQLGIGIVENALDE
                     TFAIEAADFRGIEALDARLLDLARKNMPSIPIDRLDLLIVDELGKDKSGTGLDTNIIG
                     RMGILGQEDSDGPDISYIIACSLTKASGGNALGMGLADFITQDFYDEIDFPVTYENVM
                     TSSFIKRGRMPIVARDEAQAVEWALRAMGPKNPDDILVMRIKSTLELGNLQVSPATME
                     KIMQGPVFKEREIQVLGEATPIVDKDGRLKSL"
     gene            2219..3481
                     /locus_tag="VFD89_00025"
     CDS             2219..3481
                     /locus_tag="VFD89_00025"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HZJ56614.1"
                     /translation="MVRLLIIADDFTGALDTGVQFSKMGISTQVFLYDDVDWGNIEKH
                     VQVMVVDTQSRHLSSHEAYKRVFQLTKRAKEMGVKFFYKKTDSTLRGNIGSEIAALMD
                     ACHMERLPFIPAYPKAGRTTRDGIQYVDDIPLHMTAFAGDPLNPIKEANIAGIINRQT
                     GKVVHSLNMVHIERNDYFHEGKGIFVYDAVSDEDLEGICRLLTQDQGITGIAGCAGFA
                     PYIPRIFNLNTGAKDMGRATRFHKAVLIVCGSINEVSMSQVAYAENRGIKSQLLSPHK
                     MFHNDFLNNPEYETILTNAIDTLLEHGILILKTADEEGDIIFIMGAGDGSPGESTYRI
                     ITGFIGSFVRDIMKRTNIGTLVVFGGDTALGIVEGLHCTAILPQTELLTGIPISALGS
                     KLFSGTLITKAGGFGDEGTLMDIINALG"
     gene            3719..4690
                     /gene="pdxA"
                     /locus_tag="VFD89_00030"
     CDS             3719..4690
                     /gene="pdxA"
                     /locus_tag="VFD89_00030"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZJ56615.1"
                     /translation="MLIGITMGDASGIGPELILRAFKEGSLEGHFIVIGDYRILDYCS
                     RLLDIKIPFNKIGDVTEYIEGVLNIYDMGIMDEFMPEIGKISKISGYSAYKYVERATE
                     MALGGQIHAIVTLPMNKEATRLSFPDFTGHTELIAKVCGQDNYTMMLASDKLTVTHVS
                     THVSMGEAVNAVKKERILKVIELTHNSLRSFVEKPRIAVAGLNPHAGEGGAFGREETD
                     EILPAVQSARKLGIDAVGPISPDTVFVKAVNREYHGVVCMYHDQGHIPIKLLDFDGGV
                     NVTLGLRVIRTSVDHGTAFDIAYKGIASPKSFINAYNFARRMVSGDD"
     gene            complement(4730..6028)
                     /locus_tag="VFD89_00035"
     CDS             complement(4730..6028)
                     /locus_tag="VFD89_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZJ56616.1"
                     /translation="MEFYTVKPDDTLDAIGLAFDISPQAIVDVNGLNPEEPLVVGMNL
                     VIPTQSPTEEVTYTVQQGDTLFSIAQQFGVTVYSIIALNDLTFPFELAIGQTLIIQPA
                     GGSTPVLTIETFGYYLPISDPDAFLIRALGRYLTYVGIFDFPVTETGEITGSISPIVL
                     DAAREESVLPVPVLTNLKEGEFDSDLGRAVIATPEILNTFIENIMAFLELYDLPGVMI
                     DFENLHPEDRDLFTNFIRLLFERLHSQDKLLGVNIAAKWEDLPDRPWAGFFDYRALAP
                     YMDLAAIMTYEWGWRGGPPSPTAPLPFVRKVLDYAIASDIPPDRILMGLTNYGYDWPL
                     PYGPANPASTVTLAGVWNLGRTYDAPIIFHDDVKQPSMDYINASGIQHIAWFEDALSH
                     YNKYLLVPEYGLRGVFYWTLNLPLTATWYILSKMFNIRKI"
BASE COUNT         1726 a         1290 c         1557 g         1569 t
ORIGIN      
        1 ctttgcatac gttccgtgta ttaattatta ttatggttgt tatcatggag tacctgatct
       61 atcactatga ccaaggccaa taaaaaccca taatcttcgc cttctttaat gtctacgcca
      121 taggtgtcgg agaatgaaaa ccagcgcttg gatacctctg ccacttgttc gccgttcttt
      181 aggatggaaa agttcatccc aagaaagtta ccttggatag tgaagttacc tagggtgctg
      241 ttaatgttga atcgaggctt aaaaaaggta aattcctttt tgacctttgc cataggtcta
      301 ccggattgat agattgtata ttcggggagg aacctgagta tcttctgttc tatatagtag
      361 agttcctgtc cggccatatc atagatcctg agtttattac caagggtaaa gaccctgccc
      421 ttgaccacaa aatggtcatt tccatgttca tccttgattg taaagctgtc accaaaggaa
      481 aatattcttt gcctcactac aaaccgcatg atggaatccc cctttatgca tggattataa
      541 cggctattta tatacccgtt atagataaac aactgcaact ataaaattta tcatctgcag
      601 atggacccag tcggtctgta gtttatctgc aaacagccat gttattaggc ataaaagcta
      661 tactaatgtc tattatacac ccattagaaa aataaccaac catatatttt tcgatccaga
      721 gcataggatc aatcagccgg cgtaaaatca tcagcgacac ttctcttttt ataggccctt
      781 aattagctgt gatttcacgt atatttatga tattatacag ataagttgac ctgttatgcg
      841 cttcattact gacatgttta actagcacaa caggttaaat taagcgatat agacatggga
      901 gatgaaaagg tttatgcatg caaaggatct taaaatgata cctgtcaccc aacgttttcc
      961 gggcgataaa gtggataata ttgagaagac caccagggaa cagatacaaa aaacaggggt
     1021 cagatttaaa aagggcagcc gtattgccat tacggcagga agtcgcggta ttgccaacat
     1081 tgccaccatc acaaaagctg cggttgatga ggttaaatcc cagggagccc tgccctttat
     1141 aattccggcc atgggcagcc atgggggcgc gaccccggag ggacaaagag aagtcctaga
     1201 gcattacggt gttacggaag aggcaatggg tgcgcccata atctcctcca tggatgtggt
     1261 atcgataccc aacgaaggca atgattttag actatacatg tccaaagatg cctatgaatc
     1321 agacggagta atagttatca accgtataaa accccatact gactttcatg ggcaggtaga
     1381 atcggggttg atgaagatgt gcgttatagg ccttggaaaa catgcccagg ctctggagat
     1441 gcacagactc ggggtgtacg gactgcggga gctggtaccg aaagcggcaa ggaagattct
     1501 ggccacaggg aaaatacagc tggggattgg tatcgtggag aatgcgctgg atgaaacctt
     1561 tgccattgag gcggcggatt ttcgggggat agaggcccta gacgccagac ttctggattt
     1621 ggcgcgcaag aatatgcctt ccatccccat agaccgattg gaccttctga tagtcgatga
     1681 gttgggcaag gataagagcg gtacagggct tgataccaat ataataggac ggatgggtat
     1741 tttgggtcag gaggactcgg acggccccga tatttcctat attatagcct gttccctgac
     1801 aaaggcttcg ggggggaatg ccctggggat gggtctggcg gattttataa cccaagactt
     1861 ctacgatgag attgattttc ctgtgaccta tgaaaatgtc atgacctcct cctttataaa
     1921 gcgaggcagg atgccgatag tcgccaggga tgaagctcaa gcggtagaat gggctctgag
     1981 ggccatgggt ccaaaaaacc ccgatgatat tctagtaatg aggataaaat ctaccttgga
     2041 attgggaaat ttacaggtct ccccggctac tatggaaaaa atcatgcaag gtcctgtatt
     2101 taaggagagg gaaatacaag ttttggggga agcaacccca atcgtcgata aagatgggcg
     2161 tttaaaatcc ctttgaaggt aaacattttg cattgggaaa gttttagggg aggaatgtat
     2221 ggttaggcta ttgatcattg ccgacgattt taccggagca ttggataccg gcgttcagtt
     2281 ttccaaaatg gggatatcca cacaggtatt cctctatgac gatgtggact ggggcaatat
     2341 agaaaagcat gttcaggtga tggtagtgga tacccagagc cgacatttaa gtagccatga
     2401 ggcgtataaa agggtatttc agctcaccaa acgggcaaaa gaaatgggag tgaagttctt
     2461 ctataagaag accgattcca ccttaagggg caatattggc tccgagattg ccgccttaat
     2521 ggatgcctgc catatggaaa ggttgccctt catccccgca tatcccaagg ccggtaggac
     2581 taccagggat gggattcaat acgtggacga tatacccctt catatgacag cttttgcagg
     2641 ggatccttta aaccccataa aagaggccaa tatagcaggc atcataaaca ggcagacggg
     2701 taaagttgtc catagcctga atatggtcca tatcgagcgg aacgattatt ttcatgaggg
     2761 aaagggtatt tttgtttacg atgccgtatc ggacgaggat ctggaaggga tatgccgatt
     2821 actaacccag gatcaaggta ttacagggat agcaggctgt gcgggttttg ccccctatat
     2881 accacgaata tttaatctga atacgggcgc taaggatatg ggcagagcga cccgtttcca
     2941 taaggcggtc ctgatagtct gcggcagcat aaatgaggta tccatgtccc aggtagcgta
     3001 cgccgaaaat cggggcataa aaagtcagct cctaagcccc cataagatgt ttcataatga
     3061 ctttttaaat aatccggaat atgagactat actcacaaat gcaatagaca cccttttaga
     3121 gcatgggatc cttatactaa agactgccga tgaggaggga gatataatct tcataatggg
     3181 tgccggggac ggatctcctg gggagagtac atacaggata atcacagggt ttattggctc
     3241 ttttgtaagg gatataatga agcgtacaaa cataggcacc ttggtggtct ttggcgggga
     3301 taccgctttg gggatagtcg aggggcttca ctgtacggct attttacccc aaaccgaatt
     3361 gctcaccgga atccccatat ctgccctggg gagcaagctt tttagcggta cattaataac
     3421 caaggccggt ggtttcgggg atgagggaac ccttatggac ataattaatg cattgggtta
     3481 gttgaaactt ccttaccccc ggcattgatt tggggaaaac tgaggcttat ttcatttaaa
     3541 agctgtcgct tccgatcggg cctcctgccg gtggaatcgg cttaaggcat cccgcctgtt
     3601 ttgcatgggc ggatggttta ccccaatatt ccccaaatcg catcgggaag ctgcctttta
     3661 aattaagata atagaaagtg aattacaaaa ctgattgatg aagagaaggg ataagttgat
     3721 gttgattggc ataactatgg gagacgcaag cggtatagga cccgagctca tcttaagggc
     3781 ttttaaagaa ggcagcctag agggacactt tatcgtaata ggggattaca ggattttaga
     3841 ttattgcagc agactattgg acataaaaat acccttcaac aaaattgggg atgtaacaga
     3901 atatattgaa ggtgtgctaa atatctatga tatgggaatt atggatgaat tcatgccgga
     3961 gatagggaaa atttcgaaga taagcggcta ttcggcctac aagtacgttg aacgggcaac
     4021 ggaaatggcc cttggcgggc agatccatgc catcgtcacc ctgcccatga acaaggaggc
     4081 cacccgatta agctttcccg acttcaccgg tcataccgag cttatcgcta aggtctgcgg
     4141 ccaggacaac tataccatga tgctggcgtc ggataagctg acggtgaccc atgttagcac
     4201 ccacgtttcc atgggagaag cagtaaatgc cgtaaaaaag gaacggattt taaaggtcat
     4261 agagctgact cacaattccc ttaggtcctt cgtcgaaaaa cccagaatcg ctgtggccgg
     4321 attaaatccc cacgccgggg aaggcggcgc ctttggtagg gaggagacag acgaaatact
     4381 gcctgcggta caatccgccc ggaagttagg aatagacgca gtcggaccta tttcacccga
     4441 cacggtcttt gtaaaggcgg tgaacaggga atatcacggt gtggtatgca tgtatcatga
     4501 ccaaggccac atccccataa agcttctgga ttttgacggc ggtgtaaatg tgaccttggg
     4561 ccttcgggtg attcgaactt cggtagacca cggtactgct ttcgatatcg catacaaggg
     4621 gattgcatcc cctaagagct tcataaatgc ctataatttt gcgcgccgaa tggtgagcgg
     4681 ggatgattaa ctggtaatgg atcggcacaa taggtcatat taatcctaac tagattttcc
     4741 ttatattgaa catcttggat aatatatacc aggtagccgt cagtgggagg ttcagagtcc
     4801 aatagaagac gcccctcagc ccgtattccg gtaccaaaag gtatttgtta taatggctta
     4861 gggcatcctc aaaccatgca atatgctgta taccgcttgc gttgatataa tccatggagg
     4921 gctgtttgac atcatcgtgg aatatgatgg gcgcgtcgta ggttctgccc agattccata
     4981 ccccggcaag ggtgacggta gatgcagggt tggcgggacc atagggcagc ggccagtcgt
     5041 atccgtagtt ggtcaaaccc attaaaatcc tgtccggagg aatatcactg gctatggcat
     5101 agtccaacac ctttctcaca aaaggcagcg gggcagtggg agaaggcggg ccgccgcgcc
     5161 atccccattc ataggtcatt atggctgcca aatccatata gggggcaagg gccctatagt
     5221 caaagaaacc ggcccaaggc ctgtcgggga gatcttccca tttggcggca atattaacgc
     5281 ctagcagctt gtcttggctg tgaagccttt caaagagcag gcgaataaag ttggtaaaaa
     5341 ggtctctgtc ttcgggatga aggttttcaa agtctatcat gacacccggg aggtcgtata
     5401 gttccaaaaa ggccatgata ttttctataa aggtattgag gatttcgggg gttgctatta
     5461 cagccctacc caaatcgctg tcgaattccc cttccttaag attggttagg acagggacgg
     5521 gcaaaacact ttcttccctg gcagcgtcca agacgatggg gcttatagag cctgttatct
     5581 ctccggtttc tgtaacggga aaatcgaaga tccctacata tgttaaatat ctccctaaag
     5641 ccctgattag gaaggcatcc ggatccgaaa tcggcagata atatccaaag gtctcaatgg
     5701 tgagtaccgg ggtgctgccg ccggcgggct gtataataag ggtttggcct atagccagct
     5761 cgaagggaaa ggtaaggtcg ttaagggcta tgatgctata gacggtgaca ccaaactgct
     5821 gcgcaataga aaaaagggta tccccctgct gtaccgtata tgtaacctct tctgtcgggg
     5881 attgggtagg gatgactagg ttcatgccta cgaccagggg ttcctcgggg tttaagccgt
     5941 ttacatcaac tatcgcctga ggggatatat caaaggcaag accgatggca tctagagtat
     6001 cgtcgggctt tacagtataa aattccataa aaatcaccac caactatttc tctcttccat
     6061 tatatgcggt ataattcata tgttgaggtt cagatagata atcagtaggt cagctgaatt
     6121 tgttggcatg ataattacat tg
//
LOCUS       DASRNX010000003         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_425164,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000003 DASRNX010000000
VERSION     DASRNX010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425164"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFD89_00040"
     CDS             <1..499
                     /locus_tag="VFD89_00040"
                     /inference="COORDINATES: protein motif:HMM:NF023435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HZJ56617.1"
                     /translation="PERAMMVLDLFWADRYLSNTMAYGVEGKNYEVISGDIHDPAVDT
                     VVEAKSGDEQTWAIWHNWVGPLWDQWSSNWNTTEALQKMQDDNDNSEASKLLGFLFDP
                     EPVKQEIAQLNAIWIEAGPILGSGSVPDLDKYLEELRQRMDNADGQKVIQEVERQIEE
                     WKASN"
     gene            767..>2006
                     /locus_tag="VFD89_00045"
     CDS             767..>2006
                     /locus_tag="VFD89_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZJ56618.1"
                     /translation="MNEGVNVLDWRLEWNPENGLPTSLTRRGKPGFTFPEPMMTFLIG
                     GITPNNGRNQMYDVVGCDILGVSPQLTEVKLEKEIVYINTAFGPLHLKTGFEFKEDEV
                     WLSFAVTNHSHRDIRLRSGVWRMSALNPMDFHRYSVIAPGYSPNMNEPYRVFKKIDEY
                     REKEFIDLNCHGGAGTPGYRSGIIGVHGEGVGLVNWYLNETFSCHIESFGVEDDAVRW
                     VRKIYCPCDLKPGETLVLDGLRMGIIEGTRGDAIRSASGDFSRFAKTPAGDSTPLRIM
                     EICIGEKAGRSVFKGYDEVIKKLPEIADMGFNALQIMPAMPFPSYSTFDLMDIGITYG
                     DEAELRNLAEKAHGLGLKIICDIVLHGPFEYEPEVVLRPVSPYLKDRKDWFMRHESGE
                     PGRTYTRSLDLADPRYQIHVA"
BASE COUNT          564 a          393 c          536 g          513 t
ORIGIN      
        1 tcccgaaagg gctatgatgg tacttgatct gttctgggcg gataggtatc tgtccaacac
       61 catggcctat ggggtagaag gtaaaaacta tgaggtcatc tcaggcgata tccatgatcc
      121 agccgtggat accgtggtag aagcaaagag cggtgacgag cagacctggg ctatctggca
      181 taactgggta ggaccccttt gggatcagtg gagctcaaat tggaatacaa ccgaggccct
      241 gcaaaagatg caggatgaca atgataattc agaagcttca aaattgctgg gtttcctctt
      301 cgacccggaa cctgtaaagc aggaaatcgc acagctgaac gctatatgga tagaggctgg
      361 acctatattg ggctcaggct cggtgcctga tcttgacaaa tatctggagg aattaaggca
      421 gcgtatggac aatgcagacg gacagaaggt catacaggaa gtagaacgcc agattgagga
      481 atggaaggct tccaattagg caaatatcgc aaattaacta ggttaatcga ttaggagatg
      541 tcaagaggcg tctcctaatc aattcacctg ttgttttagt taaatatgct agtaatggct
      601 attgaacgac ctgttacatc atgaaggtat aagtcatccg ctttgcaata gtatattccc
      661 tttatcatga atcagcataa cgcatttact taattaggct atattattgc attgcataaa
      721 gaggacccta cagaatatat ttttgacttg gaaggggaat tgacagatga atgaaggagt
      781 aaatgtttta gattggaggc tggaatggaa tccggaaaat gggctcccga cctcccttac
      841 ccggagaggg aaaccgggat ttactttccc ggagcccatg atgaccttcc ttataggcgg
      901 gattacaccc aacaatggtc gcaatcagat gtatgatgtg gtgggttgcg acattctcgg
      961 tgtcagccca cagcttacgg aagtgaaatt agaaaaagaa atcgtctata ttaatacggc
     1021 ctttgggcca ctacatttaa agacgggatt tgaatttaaa gaagatgagg tttggctatc
     1081 ctttgctgtc accaaccatt cccataggga tataagattg cgtagcgggg tatggcgcat
     1141 gagcgcccta aaccccatgg attttcatag gtattccgtt atcgcaccgg gatatagccc
     1201 caatatgaat gagccatacc gggtgttcaa gaaaattgac gagtataggg aaaaggaatt
     1261 tatcgatcta aattgccatg ggggtgccgg aacccctggc taccgttcgg ggataatcgg
     1321 cgttcacgga gaaggggttg gattggttaa ttggtattta aatgaaacct tttcatgcca
     1381 tattgaaagc tttggggttg aggatgacgc ggtgaggtgg gttcgaaaga tatattgtcc
     1441 ctgcgattta aagcctggag agaccctggt actggatggc cttcgcatgg ggattataga
     1501 gggcacacga ggtgatgcta ttaggtctgc atctggggac ttttcccgtt ttgctaaaac
     1561 tcccgctggg gatagcaccc ctttgcggat aatggaaata tgcatcgggg aaaaggcagg
     1621 tcgaagcgtt tttaaaggat atgatgaggt aataaaaaag ttgcccgaga ttgcggatat
     1681 gggattcaat gcccttcaaa tcatgcctgc catgcccttt cctagctatt caacctttga
     1741 cttaatggat attggaatta catacgggga tgaggcagag ctaagaaacc tcgctgaaaa
     1801 ggcccatggg ctgggtctaa agattatatg cgatatagtg ctgcatgggc catttgaata
     1861 cgagccggaa gtggtattaa ggcctgtttc gccctacctt aaagatcgca aggattggtt
     1921 tatgcgacat gaaagcgggg aaccgggccg tacttacacc agatccttgg acttggccga
     1981 cccacgctat cagatccatg tggcgg
//
LOCUS       DASRNX010000004         3542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_690969,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000004 DASRNX010000000
VERSION     DASRNX010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690969"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1543
                     /locus_tag="VFD89_00050"
     CDS             74..1543
                     /locus_tag="VFD89_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013042783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZJ56619.1"
                     /translation="MSGNKTPNVILVYADDLGYGDLSCYGGVGVDTPNVDKLLENGVK
                     FTGGYSTSAVCTPARYSLLTGEYPFRNPRTYILPGDASCIIDREKLTLPKVFKNAGYN
                     TAVVGKWHLGLGDGNLDWNGEITHTPNDVGFDYSFIFPATNDRVPCVYIENRRVKGLD
                     PDDPIEVSYTAECPYDDIPTATRNPELLRMRHSYGHDQSVVNGVGRIGYMRGGGDALW
                     RDEDLAEDFLAKVKGFISENSENPFFVFYALHQPHVPRVPNPRFVGASGLGPRGDVIA
                     ELDWCVGELLDHLEDEGLLEDTIIIFSSDNGPVLDDGYLDDAPRLNRARRHRPAGPLR
                     GGKYSKFEGGARIPLIVSWASHIRPQTSAALVSQVDLMASFASMLNIDLGDWAPDSLD
                     MIDVLLGRSHNGREEIFAEDIAKSHFLRQGKWTYLQPKEGPAINFHTDTELGTSLDPQ
                     LYNMEYDIGQRENVAQWHMDIAQKMDHRIEEIKAGERTR"
     gene            1856..2617
                     /locus_tag="VFD89_00055"
     CDS             1856..2617
                     /locus_tag="VFD89_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZJ56620.1"
                     /translation="MPLSVNVEAVFSGWDFFEGMEAVKRAGLSAYEFWTWWNKDIDEI
                     AKVGNELGLETTAICTKFISLVDPDKRQEYKDALKDTLEVAGTLGCKTIISQVGDEIL
                     GIPRETQKMSIVEGLKECAPMLEKAGVTLVFEPLNTLVDHHGYYLWSSEEAFDIECMV
                     RSTNIKVLYDIYHQQIMEGHLIYRIIKNIDRIGYFHAAGNPGRHELTTGEINYPEVFK
                     AIGKAGYKGFIGLEYFPLGDPVKGLELLVSQMEQV"
     gene            2820..>3542
                     /locus_tag="VFD89_00060"
     CDS             2820..>3542
                     /locus_tag="VFD89_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ56621.1"
                     /translation="MFRIGIIGTENSHAMAFARLINLPDKGTGRRLWEDAKVVGVYGP
                     DLQSARAVLKDGEAEFIASSPEEFIGRVDAMMITSRRGSVHYDYALPFIDKGLSVFID
                     KPFTTDTDEAEMLVERARKTGALLAGGSGCKYSWDILILKNRVEELRKQGALLGASMN
                     FAADLESEYDGFHFYAPHLTEMALTMFGSKVKGIRATENNGSVVTVVHYDDCDVTLHY
                     TKGSIMSSCLLYGKESNYYREVD"
BASE COUNT         1016 a          708 c          954 g          864 t
ORIGIN      
        1 aatatgtggt ataaatgaag gtataggaaa gataacagta gaattataca tacatatgtg
       61 aaggagtgat attatgtctg gaaataagac ccccaatgtg atccttgtct acgccgatga
      121 tttaggctat ggggatctgt catgttacgg cggtgtaggc gtggataccc ccaatgtgga
      181 taaacttttg gaaaatgggg tgaaatttac cggcggatat tcgacatcgg cggtctgtac
      241 gccggcgcgg tatagccttc tgacggggga atatcccttt agaaacccca gaacctatat
      301 cctgccggga gatgcatcct gtattataga tagggagaag ctgaccctac caaaggtatt
      361 taaaaatgcg ggatataaca cggcggtcgt gggaaagtgg catctcggtt tgggagacgg
      421 caatctcgat tggaacgggg aaatcaccca tacccccaac gatgtcggat tcgactattc
      481 ctttatcttt ccggctacca acgatcgtgt cccctgtgta tacatagaga acaggagggt
      541 aaagggacta gatcctgatg accccataga ggtgtcctac accgctgaat gtccctacga
      601 cgatatccca actgcgacca gaaatcccga acttttaagg atgaggcata gctatggcca
      661 tgatcaaagc gtagttaacg gtgtaggcag gatcggctat atgcgcggcg gcggggatgc
      721 cctttggagg gacgaagatt tagcggagga ttttttggcc aaggtgaagg gctttataag
      781 tgaaaatagc gaaaatccat tctttgtctt ctatgcgctg caccagcccc atgtaccgag
      841 ggtgcccaat cccaggtttg tgggtgcaag cggactaggt ccccggggag atgtaattgc
      901 agaattggat tggtgtgtgg gggaactatt agatcatctg gaagatgagg gattattgga
      961 agataccatt atcatatttt ccagcgataa tgggcctgtt ttagacgacg ggtatctgga
     1021 tgatgcgcca aggctcaaca gagcacgcag gcatagaccc gccggacccc tccgcggggg
     1081 taagtacagc aaatttgaag gcggcgccag aatcccccta atcgtttctt gggcttcaca
     1141 tataaggccg cagacctccg ctgcattagt tagccaggtg gaccttatgg cttcctttgc
     1201 atcgatgctg aatatagacc ttggggattg ggctcccgac agcctggata tgatcgatgt
     1261 cttactgggc agatcccata acgggaggga agagattttc gcagaggaca tagccaagag
     1321 ccatttttta aggcagggca aatggacata tctacagcct aaggaaggcc ccgccataaa
     1381 ctttcatacg gatacggaac ttggcacatc ccttgacccc cagctctata acatggaata
     1441 tgatataggt cagcgggaaa atgtcgcgca atggcatatg gacatagcac aaaaaatgga
     1501 ccaccgcatt gaagagataa aggccgggga aaggacgaga taaccccgta tattttatct
     1561 gatatgctat gaaatgcagg cataggatag gcattgatag gataatatat gtatccttat
     1621 ctatatagaa agaatatgct gtaaacaggg gctctgtcag gcaccctatt atgggaaata
     1681 tcataataca ttcttcgtta tagggattga tttgattata gataaacaac agggaaatcg
     1741 aatctgttgg catgacaatt acaatggatc tatgcgattt caagataatg aatttttagc
     1801 cggggttgtt tatctataag tcttatttaa aaaacagata aaagggggaa ctaaaatgcc
     1861 tttgtccgta aatgttgagg ccgtctttag cggctgggat tttttcgaag gtatggaggc
     1921 cgtaaaaagg gctggattaa gtgcctatga attttggacc tggtggaaca aggacatcga
     1981 cgaaatagcc aaggtcggga atgagttggg tctggaaacc acagctattt gcacaaagtt
     2041 tatcagtttg gtggatcccg acaagaggca ggaatacaag gatgccctga aagataccct
     2101 agaagtagca ggaacccttg gatgcaagac cattatatcc caggtcgggg atgaaatcct
     2161 cggcatccca agggaaaccc aaaaaatgag catagtcgag ggattaaaag aatgcgcccc
     2221 tatgctagaa aaggcggggg ttaccctggt ctttgaaccc ctcaatacct tggtagatca
     2281 tcatgggtat tatctgtggt cctcggaaga ggcctttgat atcgaatgta tggtaaggag
     2341 cacaaatatt aaggtactct acgatatcta tcaccaacag attatggaag gacatctcat
     2401 ctacaggatt ataaagaata tagacaggat cgggtatttt catgcagcgg ggaacccggg
     2461 aaggcacgaa ttaacgaccg gggagataaa ttatcctgaa gtttttaaag ctatagggaa
     2521 agcggggtat aagggtttta ttggccttga atactttccg ttgggggacc ccgtaaaggg
     2581 gttagagttg ctggtttccc agatggaaca agtataacgg gacagaaagg tgcccgcggt
     2641 ttctacaggg gacagggccc caattgatat agcgggcggg catataccct gcacctttct
     2701 gggagtagtg tgttgtaagt gtcaatacga ctgtttctga tactataatc aggaatagga
     2761 aaacataatg ctttcagata gttattaaca agctaaatca tgaaaaggag gtcacctaca
     2821 tgtttagaat tggaatcatc ggaaccgaga acagccatgc tatggcgttt gcccgcctta
     2881 taaacctacc ggacaagggc acaggccgcc ggctttggga agatgccaag gtggtggggg
     2941 tttatgggcc ggatttacaa tcagcccggg ctgtcctaaa ggatggggag gcagagttta
     3001 tcgcatcatc tccggaagag tttataggtc gggtggatgc aatgatgatt acttcacgaa
     3061 gaggttccgt ccactacgac tacgccctac catttatcga taagggcttg tccgtattta
     3121 ttgataagcc attcaccacc gatacggatg aggccgaaat gctggtagaa agggcccgga
     3181 aaaccggggc tctattggcc ggagggtcag ggtgcaagta ctcatgggat atactcatac
     3241 taaaaaatag ggtagaggaa ctgcgaaagc agggcgcgct gctgggagct tccatgaact
     3301 ttgctgcgga cctagaaagc gaatacgacg gatttcactt ctatgctccc cacctaaccg
     3361 agatggcttt gaccatgttc ggaagcaaag tgaagggtat aagggctacc gaaaataacg
     3421 gttcggtggt aactgttgtc cattatgatg attgtgatgt gaccctgcac tatacaaaag
     3481 gcagcatcat gtccagctgt ttgctatatg ggaaggaatc taactactac cgagaagtgg
     3541 at
//
LOCUS       DASRNX010000005        17713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_54048,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000005 DASRNX010000000
VERSION     DASRNX010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17713
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54048"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..754)
                     /locus_tag="VFD89_00065"
     CDS             complement(59..754)
                     /locus_tag="VFD89_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZJ56622.1"
                     /translation="MENELMLLDTHVHTSQVSPCGKVAALEMIELYKEAGYDAVVITD
                     HYHEWYFESLGDLPWEEKVQSFLSGYKSALERGKALGVRVFLGMEIRFVKGPEDYLVY
                     GLDGDYLIQNPNLHLYTLEEFRDHIQETGALIFQAHPFRVGQTPAPGNLLDGMEVHNA
                     NPRHDSGNDKAYAYAKSNDLMMGSGSDAHRPEDVGRGGIIVPRSVDSMEMFVKWYRQG
                     PAGTGHIYKNYEA"
     gene            complement(757..2169)
                     /locus_tag="VFD89_00070"
     CDS             complement(757..2169)
                     /locus_tag="VFD89_00070"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZJ56623.1"
                     /translation="MEDVKGESLEKTYGYVWDKYTGENLDGLFDFSEGYKAFISKCKT
                     ERECTAEFIRLAEDRGYQDLAALMEDNQPLKPGDKVYANNADKTLALFVIGSRPMEEG
                     LRIIGAHVDSPRLDLKQNPLYEDSGLALSKTHYYGGVKKYQWVTLPLAIHGVVVKKDG
                     SVVDIVIGEDDNDPVVGITDLLIHLAADQMDKKLSKAIEGEDLNVSMGSIPMGDKEDT
                     ARVKKNILRLLNEKYGINEEDFVSAELEVVPAGRARDYGLDRSMVMAYGHDDRVCAYT
                     SFEALMDIENPDKTCLALMVDKEEIGSVGATGMTSRFFENTMAEIMNLSGDYSELKLR
                     RCLANSKMLSGDVSAAFDPNYPTVSEPLNTAYLGKGLTFNKYTGVRGKSGSNDANPEF
                     IAQLRAIMEKHDVMWQTSELGKVDQGGGGTIAFILAEYGMDVIDCGVPLHNMHAPWEV
                     ASKADIYETMRGYRAFFLEA"
     gene            complement(2192..2638)
                     /gene="rpiB"
                     /locus_tag="VFD89_00075"
     CDS             complement(2192..2638)
                     /gene="rpiB"
                     /locus_tag="VFD89_00075"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HZJ56624.1"
                     /translation="MLAIGGDHAAFRFKEEIKKYLDERGIAYKDFGSYSEESTDYPIY
                     GEAVAKAVARGEFEKAILICGTGVGVSLAANKVKGVRAVVCSDCYTAVLSREHNDTNI
                     LALGARVVGIGLAKMIIDSWLKAEFEGGRHRKRVDMITEIEEKNLE"
     gene            complement(2715..3272)
                     /locus_tag="VFD89_00080"
     CDS             complement(2715..3272)
                     /locus_tag="VFD89_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZJ56625.1"
                     /translation="MKYYRIRMIHNDIINSDADLALPEGYYFRDFEYGDETIWADIET
                     AAGEFSSKAKALKRFSDEFGGLGTELAGRCFFLYDENHTPIGTSMGWYDKDFQGEEIG
                     RLHWIAIVPGYQGRGLGKPLVAKAIRRIQKSHKRAYLTSQTTSWKAINMYLDFGFRPL
                     YAFDDCPKAWELLADKLKHPALGRD"
     gene            complement(3311..4801)
                     /locus_tag="VFD89_00085"
     CDS             complement(3311..4801)
                     /locus_tag="VFD89_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HZJ56626.1"
                     /translation="MSFKVAFIGAGSIGFTRGLLKDLLSVPEFHDIQVAFTDINEHNL
                     SMVTQLCQRDIDANELDIKIISTTDRREALRDARYVFNVVRIGGLEAFELDVDIPLKY
                     GVDQCVGDTLCAGGIMYGQRGIPAMLDFCKDIREVSEPGCLLLNYANPNAMITWACNK
                     YGGVNTIGLCHGVQGGHQQIAEVLGLDQDEVDIICAGINHQTWYIQIKHKGVDMTGRL
                     LKAFEEHPEYSRTEKVRIDMLRRFGYYSTESNGHLSEYLPWYRKRPEEIMDWIDLGRW
                     IDGETGGYLRVCTEGRNWFETDFPNWLKEEPRKYDPNDRSQEHGSYIVEGLETGRVYR
                     GHFNVVNNGTITNLPDDAIIEVPGYVDYNGVNIPKVGDLPLGCAAICNSSISVQRMAV
                     EAAVNADDTLLRQAMMMDPLVGAVCNPPEIWQMVDEMLVAQAQWLPQYGDAIDKAKER
                     LDRGNLIPAKENYKGAARLKTKTVEEMAQDRDAATQNAGAADKAID"
     gene            5289..5435
                     /locus_tag="VFD89_00090"
     CDS             5289..5435
                     /locus_tag="VFD89_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56627.1"
                     /translation="MTRTIIPAAPDAKPLLMNTRPDAEGVEVAAMIRLVGAAGVAVIM
                     PING"
     gene            complement(5608..5979)
                     /locus_tag="VFD89_00095"
     CDS             complement(5608..5979)
                     /locus_tag="VFD89_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZJ56628.1"
                     /translation="MKFKIAHTNFNVMDLEKSVQFYKEALGLVEERRVEAEDGSFTLV
                     FMGDGVTGHQVELTYLRDREEPYDLSDNEIHLALVVRDIDAARKHHREMGCICFENEG
                     MGIYFIEDPDGYWIEIVPERR"
     gene            complement(6038..6778)
                     /locus_tag="VFD89_00100"
     CDS             complement(6038..6778)
                     /locus_tag="VFD89_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZJ56629.1"
                     /translation="MKDDVARIYYLYHSGFAVKYRDNLLIFDYYSDESESGLRGLKAG
                     TISRKDLRDADKVFVFVSHSHEDHYNPVIYDWKEINEDIHYFLSFEVDAPTRNLRYHN
                     MHPYEEYNSDTISVRTYGSTDLGVSFVASIDGLNIFHAGDLNCWYWYYESTPQELEED
                     EASFLREVDRIGDVSVDIAFFPVDPRLREYYYMGGEYFIKRLKPKVFIPMHFGTEYEI
                     TKAFAQKVKSLDTTRVVELSHRGQEIIF"
     gene            7422..8459
                     /locus_tag="VFD89_00105"
     CDS             7422..8459
                     /locus_tag="VFD89_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010167379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZJ56630.1"
                     /translation="MNSLERVSLILKHKEADHVPVYPLINSISRNYTGIDYATWCQDT
                     DLCAESILKATDDLGVDVICSLVDLSVEAADFGQPIEYPKNEAARPVYNHRIINSIEE
                     YKNVEPINPRKTHRMGEHIRLCDILMKAKGKEKPVVAFVFGPLGIVSMMRGQQEMFMD
                     ALDDPESIHIALEAVTQTLLEYCAALMETGVHAIMFDTLYASQSIMSKQMWDALEGPY
                     MERLADHVHSKGCMVMIHNCGTGIYFDVQIKRMRPQAISFLHIPDDCGSYAETKEKYG
                     HQTTLIGHVPPSLILTATSEEVENECKRQIDTFKKDGGFILATGCEYPANADVDNARV
                     MIKTAQTYGRY"
     gene            complement(8548..9240)
                     /locus_tag="VFD89_00110"
     CDS             complement(8548..9240)
                     /locus_tag="VFD89_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent methionine synthase"
                     /protein_id="HZJ56631.1"
                     /translation="MDTVILDSVDFRLDIDRILEKLHMDRESEGIEDLLRLIKIAEDL
                     GRPKVLYKEAVIEDKGHDYIVADGVKLGSRLLRENLDTVDRIYPYICTCGRELYDWAK
                     SLDDILEQYWMDKIMEAVLEGAIVAFQNHLKANYGLDSVSNMNPGSLEGWPINQQSEL
                     FAILGDPFGKIGVELTDSFLMLPIKSISGIRFSSEVDFVNCQLCPRERCENRRAQFDP
                     HLYETWELEVGS"
     gene            complement(9327..12464)
                     /gene="ebgA"
                     /locus_tag="VFD89_00115"
     CDS             complement(9327..12464)
                     /gene="ebgA"
                     /locus_tag="VFD89_00115"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017246745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HZJ56632.1"
                     /translation="MKQTYHGKINDWENHKLMHRNRQPDRAYFIPYSTAEDALTLERQ
                     CSTRFMPLNGMWKFNYAPSPMEAPEGFYNVDYMDENWDEIEVPSCWQYKGYGRPHYTN
                     IMYPFPVDPPMVPSENPTGSYIREFYLPKDWDGRRTFLRFEGVDSAFHVWVNGQEAGF
                     SKVSRMPSEFDITQLVKPGNNTIAVRVYQWSDGSYLEDQDMWWLSGIYRDVYLLSRPN
                     TMIEDFFVKTHLDDGYKDGVLEVETRLKNNGSAGIEGYSFHMVLLDDSQNLIGETSCE
                     SISLGADRKTILDLRIPADNPKKWSAEHPYLYTAVLTLKDADGNIVEVAANKVGFRTI
                     ELKNGVFLVNGVAIMLKGVNRHDHHPEGGRTISLEAMEQDVLIMKRHNINAVRTAHYP
                     NDPRFYDLCNEYGLYVIDETDLETHGFDIIGNVDQLSDDPDWEEAYVDRAKRMVERDK
                     NHPSIIMWSLGNESGFGCNHAAMGEWIRSKDPGRLIHYEGETARCFKGHKDEIKVADV
                     YSTMYTSVEEMIEVGKRDDFEHPHIMCEYAHAMGNGPGGLKEYWEAFYTYKRLQGGFI
                     WEWIDHGIPMETEDGVKYYGYGGDFGDYPNDGNFIVDGLLLPDRTPTPGLIEYKKIIE
                     PVIAEEIDLNKGLIRLTNRYDFLGLDGLTLAFNVMADGRVLQSGRVTIGDIPAGESRE
                     TLIPFTMPENPLGGTEYWLNLGFVLAHDTNWAKQGHEVAWAQFKLPVDIPRPAPVSIQ
                     SMPPIKCNDLGNNLRIAGMDFELVFDKVKGLIKTWTYQGGDMIDKGPRLNFWHAPTDN
                     DRNSTEDWEKTGLKYLTHRVDCLNWDLIEDKVVVIKVKSRIAPPVLAWGINAEYTYRV
                     YGSGDVLLEVKGEPQGEKLPKMLPRIGLQMAIPGKMDKVTWYGRGPGESYPDSKAANG
                     FGVYDKTVDELYTPYTYPQENGNRSDVKWMSITDTLGTGLLAVGLPQLDFSAHRFTAE
                     DLENAKHTCDLVPRDEITLNLDYKHNGLGTASCGPGQLPEYQLKPEAFTFALRLTPYS
                     KSGISPAQLAKRIIE"
     gene            complement(12529..13515)
                     /locus_tag="VFD89_00120"
     CDS             complement(12529..13515)
                     /locus_tag="VFD89_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009590060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ56633.1"
                     /translation="MLKVAMLSYWHVHAWDYTKQLMGRDDVKITAVWDEIPERGEKNA
                     EEVGAVFEADLDTLLGRDDVDAVVVNTPTNLHREVMVAAAKAGKHIFTEKVMALTVGE
                     CEEIAQAVQEAGVKFCISFPQRTLSHNLFAKKVIDEGLIGDVTLLRIRNAHNGSVAGW
                     LPDHFYDPVECGGGAMIDLGAHGMYLSRWLLGEPLRITSMFNNYIDKPVEDNAVSVIE
                     FKNKAIAINETGFVTSDSPFSMELYGTQGSLFIGGADNRIRLISQKAESRVPGWITPT
                     QLPDALPSAIDQWVEGILCGGEIIFGLEDGIELTRLMEGAYRSHRENSIVEI"
     gene            complement(13783..14691)
                     /locus_tag="VFD89_00125"
     CDS             complement(13783..14691)
                     /locus_tag="VFD89_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZJ56634.1"
                     /translation="MSKVKNKRVIIADLGLLLVAILWGGGFVAVKDAIVDIAPFYMIA
                     MRFGISGLIISLLYYKRIKDTKLRDIRAGILVGLFLFLGFATQTFGALYTTAGKQAFL
                     TGTNVVIVPFLTWILYKNKLDIYSAIASMLCLVGIGFLTLKGDSGMNLGDILTLACAI
                     FFAAHITLLGYWAKQADLMALTVTQMLTTAFLALFCAILFETPPSQIPRETYGSMIYI
                     IVLCTLLSFSIQTVAQKFTSANHASIILCLESVFGSIFAVIFHGDIFTPAMIIGCALI
                     LLGIITAETKLGFLAGIRILRKPLSN"
     gene            complement(14842..16248)
                     /locus_tag="VFD89_00130"
     CDS             complement(14842..16248)
                     /locus_tag="VFD89_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HZJ56635.1"
                     /translation="MKGRKIFDAITQVDDDLIGEARTTSLKKRAKTWRIWGIVAACLA
                     LALGIGQLTSIWQIKVPGRNPGGSIKPIMDVIYPKALAFDDLDAVREVRDENPVDETF
                     EAAINDFAYRTSAQIFKEKDDNINYSPLSLYYALALAASGARGDTQKELLSLLGVADD
                     ETLSEQSGNLYRLLYTDNDIGKLKIANSLWLDDDMNGEPIEFREGFVKNAIKNFFASS
                     YSVDFADENTGEAMAKWISDNTNGTLNPSIDLDKEQILSIINTIYFYDQWIDRFNRDK
                     TAMDIFHLADGNDVEVDFMNQTFASAGFARGDGFTRASLGLKNAGRMTFILPDTGISP
                     YDLLSSPEKMQEVFEDGEEFYGEVVWKVPKFKFGAEFELVNTLKALGVNSAFAQNANF
                     SGITNHMAFITKVKQETHIAIDEDGVEASAFTQIDYCGSALPKDRADMILDRPFIYGI
                     TAQNGALLFVGICGNPAK"
     gene            complement(16245..16802)
                     /locus_tag="VFD89_00135"
     CDS             complement(16245..16802)
                     /locus_tag="VFD89_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZJ56636.1"
                     /translation="MEDSKIIDLYWSRNEQAITETQTKYGALCYSIAINILYDALDSE
                     ECVNDTYNKAWQSIPPKHPNSLAAYLGRIARNLSINRWNKNHAQKRFGGTDILLSELS
                     QCIPSRQTVEGEIQSKEITKVLDCWLRSLPQEDRILFLRRYWFCEALNALADECNITP
                     NKLAGRMYRLRQSLRRELEKEDVWI"
     gene            complement(17112..17570)
                     /locus_tag="VFD89_00140"
     CDS             complement(17112..17570)
                     /locus_tag="VFD89_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HZJ56637.1"
                     /translation="MTNEEFQRIVIESLKELRDGQKTLEGRQIALEGRQIALEGRQTS
                     LEESQRTLIVGQKTLESRQAALESRQIALEGRQGAMESRQMALEEGQKETHRLLMAIQ
                     DQTAELTEFRTEANEKLDLLIEENYTIKGMVGSHEVDIRTIQRKIERRIV"
BASE COUNT         4435 a         4596 c         3609 g         5073 t
ORIGIN      
        1 tatatagaca aaagggcggt cacaccgccc ttttgtctat atacttaatt acacttcatt
       61 atgcttcata atttttatat atatgccctg ttccggccgg tccttgtctg taccatttaa
      121 cgaacatctc catagaatct acgcttcttg gcacaattat gcctcctctt cccacatctt
      181 ccggtcgatg tgcatcggac ccggagccca tcataagatc gttgcttttt gcataggcat
      241 aggctttgtc gttgccggaa tcatgtctgg gattggcgtt gtgaacctcc atgccatcca
      301 gcaggtttcc cggtgcgggg gtttgcccta cgcgaaaagg gtgggcctgg aagatcaaag
      361 ccccggtctc ttggatatgg tccctgaatt cctccaatgt gtagaggtga agatttgggt
      421 tttgaatcaa atagtcccca tccagtccgt ataccaaata atcctccggt ccctttacaa
      481 accttatctc catcccaaga aaaaccctta ccccaagggc ctttcccctc tccagggccg
      541 atttgtatcc tgacagaaag gattggacct tttcctccca gggcaaatcc cccagggatt
      601 caaaatacca ttcatgatag tgatcggtta tcactacggc atcgtagcca gcctctttgt
      661 acagctcaat catctccaag gcggcaactt taccgcaggg acttacctga ctggtgtgca
      721 catgggtatc aaggagcatc agctcatttt ccatccctac gcctccagga aaaaggccct
      781 gtagcccctc atggtctcat agatatcggc cttgctggcc acttcccatg gggcatgcat
      841 attgtgaagg ggtactccac agtctataac atccatcccg tattcggcca ggataaaggc
      901 tatggttccg ccgccgccct ggtccacctt ccccagttcc gacgtttgcc acataacatc
      961 atgcttttcc attatggccc tgagctgagc gatgaattcc ggattggcat cgttgctgcc
     1021 gctctttccc cttacccccg tatatttgtt aaaggtaagt cccttgccca ggtatgccgt
     1081 attcaagggt tcactgacag tggggtaatt aggatcaaaa gccgcgctga catcgcctga
     1141 tagcatcttt gaattagcta aacacctgcg cagtttaagt tcgctgtaat ccccagataa
     1201 gttcattatc tcggccatgg tattttcaaa gaatctggag gtcatgcccg tggctcctac
     1261 acttccgatc tcctccttgt ccaccataag ggctaagcag gtcttatctg gattttctat
     1321 atccataagg gcctcaaagg atgtataagc gcatacccta tcatcatgcc cgtaggccat
     1381 aaccatactt ctgtccagcc cataatccct ggccctgcct gcggggacga cttctagttc
     1441 cgcagaaacg aaatcctctt cgttaatacc gtatttttcg ttaaggagcc tcaggatatt
     1501 tttcttaacc ctggctgtat cctccttatc ccccatgggg atgcttccca tagaaacgtt
     1561 cagatcctct ccttctattg ccttggagag ctttttatcc atctgatccg ccgccagatg
     1621 aatcaacagg tccgttatcc ctaccaccgg atcattatcg tcctccccta ttactatatc
     1681 cacaacgcta ccatcctttt ttaccactac gccgtgaatg gccaagggta gggtaaccca
     1741 ttgatacttt ttaacgccgc cgtaataatg ggtcttagag agggccaacc cggaatcttc
     1801 atacagggga ttctgcttta gatccagtct cggtgagtcc acatgggctc cgataatcct
     1861 cagtccctcc tccataggcc ggctcccaat tacgaataaa gccagggtct tgtccgcatt
     1921 gttggcgtat accttgtctc cgggttttaa gggctgatta tcctccatca aggcagccag
     1981 atcctgatat cccctgtctt cggctaatct aataaattct gcagtacact ccctttcggt
     2041 cttacatttg gaaataaacg ccttatagcc ttcggaaaaa tcaaagagtc catccaggtt
     2101 ttccccagtg tatttgtccc atacataccc ataggttttc tctaaacttt ccccttttac
     2161 gtcctccata ctcattaaac acatctcctt atcattctag gttcttttct tctatttcgg
     2221 taatcatatc tactcttttt cggtgcctac caccttcaaa ttctgcctta agccaactgt
     2281 ctatgatcat cttcgcaaga ccgataccca caacccttgc gcccaaagcc aggatattgg
     2341 tgtcgttatg ttccctggac aggaccgctg tgtagcaatc actgcatacc acggccctaa
     2401 ctcccttaac cttgttagca gctagagata cgcccacccc tgtaccgcag atcaggatag
     2461 ccttttcaaa ttcgcccctg gcaacggcct tggcaacggc ctcgccatag atgggatagt
     2521 cggtactctc ctcggagtac gacccaaaat ccttataggc tattcccctc tcatcgagat
     2581 actttttaat ctcctcctta aagcgaaaag cagcatggtc accgcctata gccaacataa
     2641 aagcacgctc ctcataataa tctatatgaa acccaaacga tccgtaaatc cccatcaacg
     2701 ggcggcaaaa cccgtcagtc ccttcccaat gcaggatgtt taagcttgtc ggccagtaac
     2761 tcccaagcct tgggacaatc gtcaaaggcg tacaggggcc tgaatccaaa atccaagtac
     2821 atgttgatag ccttccagct agtggtctgg gaggtaaggt aggccctttt atgggatttc
     2881 tggatccgtc ttatggcctt ggctaccagg ggttttccga ggccccttcc ctggtagccg
     2941 ggtactatcg caatccagtg caatctgccg atttcctccc cctgaaaatc cttgtcatac
     3001 cagcccatag atgtgcctat gggtgtgtga ttttcgtcat agagaaaaaa acatctgccg
     3061 gccagctctg tccccaaccc gccgaattcg tcagaaaacc ttttcagagc cttggccttg
     3121 ctgctgaact caccggctgc tgtctcgata tccgcccaaa tcgtctcgtc cccgtactcg
     3181 aagtctcgaa aataatagcc ttccggcaga gctaaatctg catcgctatt tattatgtcg
     3241 ttatggatca tccttattcg gtaatatttc accgcatcta cctcccaaaa aatcagctat
     3301 catcttaaaa tcaatctatc gccttatcgg ctgccccggc attttgcgta gctgcgtcgc
     3361 ggtcttgtgc catctcctct acagttttgg tctttagcct ggcagcaccc ttgtagtttt
     3421 ccttggctgg gattaaattg ccccgatcaa gcctttcctt tgccttatct atggcatccc
     3481 catattgggg cagccattgt gcttgggcta ccagcatctc gtccaccatc tgccaaatct
     3541 ccgggggatt gcatacggcc ccgaccaacg gatccatcat catagcctga cgcaataggg
     3601 tatcatcggc attgaccgcc gcctctacgg ccatcctctg taccgatatg ctggagttgc
     3661 atatggccgc acatcctagg ggaaggtctc ctacctttgg aatgtttaca ccgttataat
     3721 ccacataccc cggcacttcg ataatagcgt catcgggcag gttggttatg gtaccgttat
     3781 ttactacatt aaagtgtccc ctgtataccc gtcctgtttc aagcccttct actatatagg
     3841 aaccatgctc ctggctcctg tcgttgggat cgtattttcg gggttcttcc ttcagccagt
     3901 tgggaaagtc cgtttcaaac caattgcgtc cttcggtaca gacccttaga tatcccccgg
     3961 tttccccatc tatccatcta cccaaatcta tccagtccat gatctcttcg ggccgctttc
     4021 tgtaccatgg aaggtattcg ctgaggtgcc cgttggattc ggtgctgtaa tacccaaaac
     4081 gcctgagcat atctatacgc accttttcag ttctgctgta ttcgggatgt tcttcaaaag
     4141 cctttaagag cctccccgtc atgtccacac ccttatgctt aatctgaata taccacgttt
     4201 gatggtttat gccggcgcag atgatatcca cttcgtcctg atccaatccc aggacctccg
     4261 caatctgttg atgcccaccc tgaacgccgt gacatagccc gatggtgttt actccgccgt
     4321 acttgttgca cgcccaggtg atcatggcgt tggggtttgc gtagttaagg agcaggcaac
     4381 cgggttctga aacctccctt atatccttgc aaaaatccag cattgcgggg atgccccgct
     4441 ggccgtacat gataccgccg gcgcatagag tatccccgac gcattgatcc acgccatatt
     4501 ttaggggaat atccacatcc aattcgaagg cctcaaggcc ccctatccta accacattga
     4561 atacgtatct cgcatccctc agtgcctcac ggcgatccgt agtagatata attttgatat
     4621 caagctcgtt ggcatcgata tccctttggc agagctgggt aaccatggat aggttgtgct
     4681 cgtttatatc cgtaaatgcc acctgtatat cgtgaaattc cggtactgag agcaggtcct
     4741 tgagtaatcc cctagtaaac cctatacttc cggctccgat aaatgcaact ttaaaagaca
     4801 tataagccta cctccctatt tcataaaata ataatatcat gattcagctt taaaatgtta
     4861 ttatacggat aaacaatacg taaactaaat ctatcggcgt gataattgta acgggtccat
     4921 gcaatttcat acggtaaatt tataattgaa gttgtttatc tacagctttt taccgctact
     4981 tacagcctaa tcgattagtc tttttactgt atatggtatg gttcgccggt gaaatagagt
     5041 tttaatactc tgatcatatc ccaaaaacag agctgttata tgctttatta atgatattga
     5101 gatcctataa cctttgcagc cccaaccggt aaaatttcag tatataggaa tattgcaatc
     5161 cgacaatttt tgttctaaac tattatggcg atgctttttt cgctatggta gcggccatag
     5221 cagcagatga ttataactaa aaggggatat gcccatgcat atagaatgtc ccaattgcaa
     5281 agccttcttt gacgaggacg ataatacccg ctgccccaga tgccaaaccc ctgttgatga
     5341 atactcgccc ggatgcggag ggtgtggagg ttgcggcaat gataaggctt gtgggggctg
     5401 cgggggttgc tgtaatcatg cccattaatg gataaacaat aggtcaacta aatttatcgg
     5461 cgtgataatt gtaacgggtc tatgcaattt catgacaata aatttattag ttgaagttgt
     5521 ttttctatag tagcccaaca agcccaatac aaatttttga aaaagcaggg tcgacaatag
     5581 actctgcttt tacgattttt agtgctttta ccgcctttcg gggacaatct ctatccaata
     5641 tccgtcggga tcttcgataa agtatatgcc catgccctcg ttttcaaaac atatgcatcc
     5701 catctccctg tggtgcttgc gtgctgcatc aatgtcccga accaccagag ccaaatggat
     5761 ttcgttgtcc gataggtcat atggttcttc cctgtccctt agatatgtaa gctctacttg
     5821 atggcccgta accccatcgc ccataaatac caaggtaaag cttccgtctt ccgcttccac
     5881 ccgcctctct tctacgagtc ccaaggcctc cttgtaaaac tgtacgcttt tttccaggtc
     5941 catgacgtta aagttggtgt gtgcaatctt aaatttcata tcttctgtcc ccttccttta
     6001 atatatattt gaaattggtt cttacggctg cccattccta gaatataatt tcctgccccc
     6061 tatgggataa ttccactacc ctggttgtat ccagggattt gaccttttgc gcaaaggcct
     6121 tggttatctc gtattccgtt ccaaaatgca tcggaatgaa aactttgggt ttaagccttt
     6181 ttataaaata ttccccaccc atataataat actccctgag cctggggtca accggaaaga
     6241 acgcaatgtc aacggataca tccccaatcc tgtccacttc ccttaaaaag cttgcctcgt
     6301 cctcttccaa ttcctggggt gtcgattcat aataccaata ccaacaattg agatccccgg
     6361 catggaagat atttagccca tctatggaag ctacaaagga aacccccaaa tccgtggaac
     6421 cgtaggtcct aacgctgatg gtatcgctgt tatattcttc atagggatgc atattatggt
     6481 accttaaatt tcgcgttggt gcatccactt caaagcttag aaaatagtgg atatcttcat
     6541 taatttcctt ccaatcatag atgaccggat tgtaatggtc ttcgtggctg tgggacacaa
     6601 atacaaaaac cttgtcggca tcccttaagt ccttgcggga aatggttcct gctttgagcc
     6661 ccctcaatcc cgactcggat tcgtcggagt aataatcgaa aatcaacaaa ttgtccctat
     6721 atttcacggc aaatccgctg tgatataggt aatatattct cgccacatca tctttcaata
     6781 tccgtcactc tcccaattaa aatattcaaa acagtagcaa agactgccgc accgcactgg
     6841 acccggcggg gcaagaaacg gttttgccat agagctatgc cctttttaaa acatataaac
     6901 tagaaagatg cttttacggc gtccgggtct caatgcagaa atttatcgta aaacatatac
     6961 taatatataa cacccacacg gtgctttaaa ccgtctatag gaaaataact atttctatcg
     7021 ctctccgtct acgacagaag gaaaagccac ccctattgct tcattttgta tacccccaga
     7081 aacaaaagaa ccgtgtccac tgcttcctgt tgcttcgttc tcattgcctt attaactgct
     7141 agaagcaaag gaactatagc cgctgatttg ccatcacttc ctcggaagca ggggaaccga
     7201 ccctgccgct tcccgtttcc cgccacttcc caccatcgtg cgagaagcac gagaactgcc
     7261 cctgccgctt cctttctaga taaaagaata caaaatatca aaatatagta atataatcag
     7321 catgcattgg tatgataagg tgaaatagga agcactcaga cttgcataat acattttatc
     7381 tttaaagaaa aatattatac atgatccaag gaggagtaga catgaattca ttagaaaggg
     7441 tatcccttat tctaaagcat aaagaagccg accatgtgcc ggtctatccc cttatcaaca
     7501 gcatatcccg aaactacacc ggtattgact acgcaacctg gtgtcaggac acggacctgt
     7561 gtgccgagtc catattgaag gccaccgatg atctcggagt ggatgttatc tgctcattgg
     7621 tggatctgtc cgtagaagcg gcggatttcg gtcaacccat tgaatatcct aaaaatgagg
     7681 cagcacggcc cgtatacaac catagaatta tcaattccat tgaagagtat aaaaatgttg
     7741 aaccgataaa ccccaggaaa acccatagga tgggcgaaca tatccgtcta tgcgatatac
     7801 tgatgaaggc caaaggcaag gaaaagccgg tggttgcctt cgtctttgga cccttgggca
     7861 tcgtgagcat gatgaggggc caacaggaga tgtttatgga tgctctggac gatccggaat
     7921 ccatacacat tgccttagag gcggttaccc aaaccctttt ggaatattgt gccgccctta
     7981 tggaaaccgg ggtccatgcc ataatgtttg acaccctgta tgcctcccaa tccattatga
     8041 gcaagcagat gtgggatgca ctggagggtc cttatatgga acgcctggcc gatcatgtcc
     8101 attccaaggg gtgtatggtc atgatccaca actgcggaac cggtatctat tttgatgtac
     8161 agattaaaag gatgcggccc caggccattt cgttcctcca catccccgac gactgcggct
     8221 cctacgccga gaccaaagaa aagtacggac atcaaaccac ccttataggc catgtgcccc
     8281 caagcctcat ccttaccgct acttccgaag aggtagagaa tgagtgtaaa aggcagattg
     8341 atacctttaa aaaggacgga gggtttatcc tagccacggg ctgcgaatat cccgccaacg
     8401 ctgacgtgga caacgcaagg gttatgatta agacggcaca aacctacgga aggtactgat
     8461 atccagactt tcgccctccg tcctttaatt tctaacttcc aacttctaac tgccaacctc
     8521 taatttccag cttccaactt ccaacctcta acttccaact tctaattccc aggtctcgta
     8581 gagatggggg tcgaattgcg ccctgcggtt ttcacacctc tccctggggc agagctggca
     8641 gttgacaaag tccacctcgg aggagaacct aatgccggat atggacttta taggcagcat
     8701 caaaaaacta tcggtgagct ctacgcctat cttcccaaat ggatccccaa ggatggcgaa
     8761 gagctcgctt tgctgattta taggccagcc ttccaaggaa ccgggattca tattggatac
     8821 ggaatcgaga ccatagttgg cctttagatg attttggaag gctactatgg ccccttccag
     8881 gaccgcttcc ataatcttat ccatccagta ttgctccaat atgtcgtcaa gggatttggc
     8941 ccaatcatac aattccctgc cgcaggtaca gatatagggg tatatcctgt ctacggtatc
     9001 taagttttcc ctgagcagcc ggctgccaag cttcaccccg tctgcgacta tataatcatg
     9061 gcctttatcc tcaatgaccg cctctttata taataccttg ggcctgccca gatcctcggc
     9121 aattttaatc agcctgagta aatcctctat gccttcactt tccctgtcca tatgcaactt
     9181 ttcaagtatg cggtctatat ccaatctaaa atctacggaa tctaaaataa cggtatccat
     9241 gcttatgatg tcccctttca tatccgggaa ataatactgg gccggcagag ccggcccata
     9301 tttctgcgct gttattaatt actcccttat tcaataatcc ttttggcaag ctgtgccggg
     9361 gaaatcccgc tcttactgta gggggttaac cttaaggcaa aggtaaatgc ctcgggcttt
     9421 agctgatact caggcagctg tccggggccg cagctggcgg ttcccaatcc gttatgcttg
     9481 tagtcaaggt taagggtaat ctcatccctg ggcaccagat cgcaggtgtg tttggcattt
     9541 tccaaatctt cggcagtgaa tctatgggcg ctaaaatcca gctgtggcag ccccactgcc
     9601 aacagacccg tccctaaggt atcggttata gacatccatt ttacatcgga tctgttcccg
     9661 ttctcctggg gataggtata gggggtgtag agttcgtcca cggtcttatc atatacccca
     9721 aaaccgtttg ccgccttact gtcgggatag gattctcccg gcccgcgacc gtaccatgta
     9781 accttatcca tcttaccggg tatggccatt tgaagaccta ttcggggtaa cattttgggc
     9841 agtttctcgc cttggggctc tccttttact tccaataaaa cgtcgccgct tccgtatacc
     9901 ctgtaggtat actcggcatt tatcccccat gccagtacgg gaggcgctat gcggctctta
     9961 accttaataa ccacaacttt atcctctata agatcccaat ttaggcagtc aactctatgg
    10021 gtcaaatact taagaccggt tttctcccaa tcttctgtgg agtttctgtc gttatccgta
    10081 ggcgcatgcc agaaattgag cctggggccc ttgtctatca tgtctccgcc ctggtaggtc
    10141 caagtcttta taaggccttt caccttatca aataccagtt caaaatccat acccgctatc
    10201 cgaaggttat tgcccaaatc attacactta ataggaggca tagattgaat gcttacaggt
    10261 gcaggccttg gtatatctac cggcaactta aactgcgccc atgccacctc atggccctgc
    10321 tttgcccagt ttgtatcatg ggccaaaaca aatccaagat tcagccagta ttccgtgccc
    10381 cctaagggat tttccggcat cgtaaagggt attaatgtct ctctgctctc tccggcgggg
    10441 atgtccccga ttgttaccct gccgctttgg agaacgcggc cgtcggccat aacgttaaag
    10501 gccagggtaa gcccgtccag acccaaaaag tcatacctgt tggtcagcct tatgagaccc
    10561 ttattaaggt ctatctcctc tgctataacg ggttctatga tcttcttgta ttcaatgagc
    10621 cccggggtgg gtgtgcgatc cggcaacaaa agtccgtcta ctataaagtt tccgtcgttg
    10681 ggataatccc caaagtctcc gccgtagccg tagtatttaa cgccgtcttc ggtctccatg
    10741 gggataccgt ggtctatcca ttcccatata aacccgcctt ggaggcgttt atatgtataa
    10801 aaggcttccc aatactcctt gaggccgccg ggaccgttac ccatggcatg ggcgtattcg
    10861 cacatgatat ggggatgttc aaaatcgtcc ctttttccta cctcgatcat ctcctccacc
    10921 gaggtataca tggtgctgta cacatcggca accttgatct catccttatg gcccttgaag
    10981 cagcgggccg tctctccctc ataatggatt aggcggccag gatccttgga ccttatccat
    11041 tcgcccatgg cagcatggtt acatccaaag ccggattcgt tccctaagga ccacattatg
    11101 atggagggat gatttttatc cctttccacc atccgctttg ccctgtccac ataggcttct
    11161 tcccagtccg gatcatcgct tagctgatct acgttaccta ttatgtcaaa accatgggtc
    11221 tcaaggtcgg tctcgtctat aacatacagt ccatattcgt tacataggtc ataaaacctt
    11281 ggatcgttgg gataatgggc ggtacgcaca gcattaatgt tatgtcgctt cattatgagt
    11341 acgtcctgct ccatagcctc tagagatatt gtcctgccgc cttcggggtg gtggtcatgg
    11401 cgattaaccc ctttgagcat aattgccaca ccatttacca ggaacacacc gttctttagc
    11461 tctatggtgc gaaatcccac tttatttgct gcaacctcta ctatatttcc atcagcatcc
    11521 ttgagggtaa gcactgctgt atagaggtag gggtgctctg ccgaccattt tttcggattg
    11581 tctgccggga tccttagatc caaaatagtt ttcctgtcgg cacccaagga tatggattcg
    11641 catgatgttt cgcctatgag gttttgagaa tcatccagta acaccatatg gaaactataa
    11701 ccttcgatcc ctgcagagcc gttattttta agccttgtct ctacctccaa taccccgtcc
    11761 ttgtatccat cgtcgaggtg tgtttttaca aaaaagtcct ctatcatggt atttggcctg
    11821 gacaataggt atacgtccct atagattccg ctgagccacc acatatcctg gtcctctaag
    11881 tagctgccgt cggaccattg gtatacccta acggcgattg tattattgcc gggttttaca
    11941 agttgggtga tatcaaattc agagggcatg cggctaacct tgctaaatcc cgcctcctgt
    12001 ccgtttaccc aaacatgaaa ggcactgtct actccttcaa agcgcaggaa tgtcctccgt
    12061 ccgtcccaat cttttggaag gtagaattcc cttatgtagc tgcccgtggg attctcgctg
    12121 ggcaccatgg gcggatcgac gggaaagggg tacattatgt tggtatagtg gggacgcccg
    12181 taacccttgt actgccaaca gctgggtact tctatttcat cccaattttc atccatgtag
    12241 tctacattat agaatccttc cggcgcttcc atgggtgatg gggcatagtt aaatttccac
    12301 attccattta agggcataaa ccgggttgag cactgcctct ctagagtaag ggcgtcctct
    12361 gcagtcgagt atggaataaa gtatgcacga tccggctgac ggtttcgatg catcagctta
    12421 tggttttccc aatcattgat ttttccgtga taggtctgtt tcatctttgg ctcccttcag
    12481 gtatttgaga cgattcgaaa aatatattat acgatcttga attcaatatc atatttccac
    12541 tatgctgttt tccctatggg atctataggc cccttccata aggcgggtaa gctctatccc
    12601 gtcctctaag ccaaatatta tctccccgcc gcataaaatt ccctcaaccc actggtcgat
    12661 ggcgctggga agtgcatcgg gaagctgggt aggggttatc cagccgggaa cccggctttc
    12721 tgccttttgg gatataagcc ttatcctatt gtcggcaccg cctatgaaca ggcttccctg
    12781 ggtcccgtaa agttccatgg aaaaggggct gtcggaggtt acaaacccag tctcgtttat
    12841 ggctatggcc ttattcttaa attctatgac gctgaccgca ttatcctcaa cgggtttgtc
    12901 tatatagttg ttgaacattg aggttatgcg aaggggctcg cctagtaacc accgggataa
    12961 gtacattcca tgggcgccta agtctatcat ggcaccgcca ccgcactcta ccgggtcata
    13021 aaaatggtcg ggtagccaac cggctacaga accgttgtgg gcatttctaa tccggagcaa
    13081 ggtgacatcc cctatcaggc cttcgtctat gacctttttg gcaaagaggt tatgggacaa
    13141 ggtacgctgc gggaaggata tacaaaactt caccccggct tcttgtaccg cttgggcgat
    13201 ttcctcacat tcccctacgg tcagagccat aaccttctcc gtaaatatat gcttccccgc
    13261 ctttgcagcg gcaaccatta cctccctatg taagttggtt ggggtattga ccaccaccgc
    13321 atcaacatcg tcccttccaa ggagtgtgtc caagtcggct tcgaagaccg ccccgacctc
    13381 ctcggcgttc ttttctcctc tctcgggaat ctcgtcccat accgccgtaa tcttcacatc
    13441 atcccggccc atgagctgtt tcgtgtagtc ccatgcatga acgtgccaat agctgagcat
    13501 tgcaaccttt aacatacctt ttctctccct tcattctata aacactttat aaacactttt
    13561 tgtatgaaat acaccttagc tttctctaaa tggttgttat aagaaggaag tttcaaaaaa
    13621 cacattatta ataacgaccg ccataataat attgattcat tttttgaaac ttcaactata
    13681 cgactactct tatgatggta ctaagcgttc aatagctatt gccgatataa tgcattagtt
    13741 tgtttgctga aattattata gcataaaaca ccaattattt cttcaattgc tcaagggttt
    13801 ccttaggatc cgaataccgg ctaaaaagcc cagcttcgtt tctgccgtta taatccccag
    13861 cagtattaag gcgcacccta ttatcatagc aggggtaaat atatccccat gaaatatcac
    13921 agcgaagatc gaaccgaata cggattccaa gcagagtatt atagacgcat ggtttgccga
    13981 ggtgaatttt tgggcaacgg tctgaataga aaagctgaga agcgtacaga gcactattat
    14041 atatatcatc gaaccgtaag tttcccttgg tatctgtgac ggaggtgttt caaataatat
    14101 ggcgcagaag agggctaaga aagcggtggt caacatctga gtcacggtta aagccatgag
    14161 gtctgcttgt tttgcccaat atcccaatag ggttatatgt gcggcaaaaa agattgcgca
    14221 ggccagggtt aaaatatccc caaggttcat accgctgtca ccctttaggg ttagaaatcc
    14281 gatacccacc aagcaaagca tggaagctat agccgaatat atatccagtt tatttttata
    14341 caatatccac gttagaaaag gcacgattac cacgttggtc ccagtcaaaa aggcttgctt
    14401 gcctgctgtg gtatacaatg ccccaaaggt ttgggtggca aagcctaaaa ataagaatag
    14461 tcctaccaaa attccggccc taatatcccg gagctttgtg tcttttattc tcttataata
    14521 gagcagggat attataaggc cggagatacc aaacctcata gcgatcatgt aaaaaggggc
    14581 gatgtcaact atagcgtcct taaccgctac aaagccgccg ccccataaga tggccaccaa
    14641 taataaaccc agatccgcaa ttattaccct tttgttcttt acttttgaca attttccacc
    14701 ccatgctgtt gatctcgcta tattcttgca tttttaattc acattgtcaa ccgccggggg
    14761 aaaagggtac catgataaat tggaaattaa tcataagttg aaacatataa agccccagaa
    14821 tccaataaaa aacttcacta tctatttcgc aggattcccg cagataccca cgaataaaag
    14881 agcgccgttt tgtgccgtga taccgtagat aaagggtcga tccagaatca tatcggctcg
    14941 gtctttcgga agggcggacc cgcaatagtc tatctgggta aaggcggaag cctcaacacc
    15001 gtcttcatcg atggcaatat gggtttcctg ctttactttg gttataaagg ccatatggtt
    15061 cgtgatgcct gaaaaattgg cattctgtgc aaaagccgaa tttaccccaa gggctttaag
    15121 ggtatttacc agctcaaatt ccgcaccaaa tttaaacttg ggtaccttcc atactacctc
    15181 accataaaat tcctctccgt cttcaaaaac ttcctgcatt ttctcaggcg atgaaaggag
    15241 gtcatagggg gaaatgcccg tatcgggcaa tataaaagtc atccgcccag catttttaag
    15301 ccccaaggaa gccctggtaa aaccgtcacc tctggcaaag cctgccgagg caaaggtctg
    15361 attcataaaa tctacctcta catcattccc gtctgcaagg tgaaagatat ccatggcagt
    15421 cttatcccta ttaaaccggt caatccattg atcatagaag tagatagtat tgatgatgct
    15481 gaggatttgt tctttatcaa gatcaatgga tggatttagg gtgccgtttg tattgtcgct
    15541 gatccatttg gccatggctt cccccgtatt ctcatctgca aaatccacac tataagagga
    15601 tgcaaagaaa ttttttatcg cattttttac gaagccttcc ctaaactcta tgggttcgcc
    15661 gttcatatca tcatccagcc atagggaatt ggctatcttt aattttccaa tatcattatc
    15721 ggtgtacagg aggcgatata ggttaccact ctgttccgat agggtttcat catccgcaac
    15781 gcctaggagg gatagtagct ctttttgagt atcccctctt gcacctgagg cagccaatgc
    15841 caaggcatag tatagggata agggagaata gtttatatta tcatccttct ctttaaaaat
    15901 ctgggcggag gttctgtatg caaaatcgtt tatcgccgct tcaaaggttt catccacggg
    15961 attctcatcc cgcacttccc gcacagcgtc taggtcatca aaggcaaggg ctttaggata
    16021 gattacatcc atgattggtt taatactgcc acccggattc cttcccggaa cctttatctg
    16081 ccagatcgag gtcagctgac caatacccag cgccaatgcg aggcaagcgg caacaattcc
    16141 ccagatcctc catgtcttgg ctctcttttt gagactggtt gttctggctt cccctatcag
    16201 atcatcgtcc acctgggtta tagcgtcaaa gatctttctt cccttcatat ccaaacatcc
    16261 tccttttcta attctctcct aaggctttgc cttagacgat acattcgtcc cgccagttta
    16321 ttcggtgtaa tgttgcactc atcggcaagg gcgtttaagg cttcgcaaaa ccaatatcta
    16381 cgaaggaata atatcctatc ctcctgggga agcgaacgta gccaacagtc tagaactttg
    16441 gttatctcct tcgattggat ttccccttcc acggtttgtc gggaaggaat acactgtgaa
    16501 agctccgaaa gcagtatatc ggtccccccg aacctttttt gtgcatggtt tttgttccag
    16561 cgattgatag ataagttgcg tgcaatacgt cctaagtagg ccgcaagtga attaggatgc
    16621 ttaggcggta tagactgcca cgccttatta tatgtatcgt taacacattc ttcactgtcc
    16681 agtgcgtcgt ataatatgtt tatggcaata gaataacaca gcgcaccgta ttttgtttgg
    16741 gtctctgtta ttgcctgctc attccgggac cagtaaaggt ctattatttt tgaatcctcc
    16801 atagatctcc cccctttcca tatggacttg aaggccttca tctaaagata aagaataggt
    16861 caactaaatt tatctatagg tgataaattt tatagttgga gttgtttatc tatatatata
    16921 gacaggaaaa aagcaaggat attcgcttta atgtgcttgt tataggcata aaattttata
    16981 aaataaaaac ccggttaagg gtttttaaaa tctgatccaa tatgacacca gaaacaatcg
    17041 ctgtagcaat tgcaacacag gtttcaacgg ggcggaggat atctttccct cacctagtta
    17101 tatttaaaca gctatactat tcgtctttct atttttctct ggattgttct aatatccact
    17161 tcatggctgc caaccatgcc ttttatcgta taattctctt ctatcagtaa atccagtttt
    17221 tcattggctt cagtcctaaa ttcagttagc tcagctgtct gatcttgtat tgccattaac
    17281 aatctatggg tctccttctg cccttcctcc aatgccattt gtctggactc catagctcct
    17341 tgcctgcctt ctaaggctat ctgtctgctc tctaaggccg cttgtctgct ctccaaagtc
    17401 ttttgcccta caattaatgt tcgctggctt tcttctaaag acgtctgtct gccctccagg
    17461 gcgatctgtc tgccctccaa ggcgatctgt ctgccttcca aagtcttctg tccgtccctc
    17521 agttccttta gcgattcaat tacaatcctt tgaaactctt cattggtcat accctaacct
    17581 cctatccttt atataatgtt tactatattt gtcattaaga aagtttcaaa aaatacatta
    17641 ttgatactcg aggtcataac accgacaatt ggacttttca aaatttcaat tcaccggtca
    17701 tttgcttgac gtt
//
LOCUS       DASRNX010000006         6812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_372821,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000006 DASRNX010000000
VERSION     DASRNX010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372821"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1727
                     /locus_tag="VFD89_00145"
     CDS             51..1727
                     /locus_tag="VFD89_00145"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56638.1"
                     /translation="MSRFIALLKASLNVHFGISSLKYRFTKEKKRLWEPIAVLIAIVI
                     GGGSILTMYSLLLLAVFMAGQALGHPEIVITLSFVASQLVILIFGIFYIISVFYFSND
                     MGILIPMPIRPGKVLGVKFITILLSEYLIALPMLLPAFIIYGAGSWQGILYWIKGLIL
                     VLTAPILPLVIAALFVVLLMRFINIRKGKDLMVVIGSLFGLMAGLGINFFTQNLPQGS
                     EEEFIKHMIESNTALIESIGNKFPPSIWATLALTLRGWHGWAYFLLFVGLSFLLVILL
                     YWIGNHFFYRGYLTGQEIRRKNKAISQQDMQKRISRASSPFLALLKREWRLFMRTPIY
                     MMNGLAGMIMFPIFLIMPFLTKSDELTEAFGYAKDPEYALIVTLVFLGIMLFASSINM
                     VSCTSISREGATFWISKVIPVAPKDQILAKLAHSSILSLVGLIIMAIPLYAFFNISLI
                     HLTILIILGLLANGLINILGLMVDLLRPKLSWNDPQEAIKQNLNVFFSLLASLLIMAI
                     LAVASIVLIVAQVNQFWVYAVLALIIIVLSVPALFGLFALAKTRYKSIEI"
     gene            complement(1924..3402)
                     /locus_tag="VFD89_00150"
     CDS             complement(1924..3402)
                     /locus_tag="VFD89_00150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HZJ56639.1"
                     /translation="MGYIWVMERSLEKYFGYNSFLSGQKNVIESILKGSNTIAIFPTG
                     GGKSLCYQLPALIFPGTTIVISPLISLMKDQVDELSELNISAAYISSILSDRQVTKTI
                     EDMKMGKYKIVYIAPERFYSEDFLVALKEIHVPFVAVDEAHCISQWGHNFRPAYLKIR
                     DLIGHIGNPILAAFTATATGKVQSDIADLLGMKDDCNVFIESFDRPNLEFKVEKCEDK
                     RTYILRHIQKNRHRPGIIYAATRANVEEIHLLLRGRGIATGMYHGGLDRFTRNDAQEA
                     FLRNDIKVMVATNAFGMGINKSDVRYIIHWNMPKSLENYYQEAGRAGRDGLASSCILL
                     HSENDYSLNRFMIEGNYPPVKLVEGLYKRIKSRDLKGIPQELILRSRSIDRHTLQSAL
                     RKLMEYNFARLNGGIVYPVFNREFDLTQEEIDWHKKIEMGRLMEIEEYCEGKKCLRRF
                     ILEYFDESVKFKNCANCSVCNRAGASKLSDRDLDKLLKDIFS"
     gene            complement(3794..5188)
                     /gene="tilS"
                     /locus_tag="VFD89_00155"
     CDS             complement(3794..5188)
                     /gene="tilS"
                     /locus_tag="VFD89_00155"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZJ56640.1"
                     /translation="MLNKVLDYIQEYDMLEPGDRVVVGVSGGADSVALIHLLWRMREF
                     IPMNLYVAHVDHGIRGEAAREDALFVEAMATEWGLDFFLKEASVPDLVKEWGVSEEQA
                     GRRVRHEFYREVRQRVGAQRIALGHHRDDQVETILYNIIRGSGLAGLSGMDPVRDGII
                     IRPLLGVSRQEIEDYCHREGLGFRHDHTNEETIYTRNRVRHMVIPMIEEHFNPNFSAG
                     LTRMGDILRDDEEFLRSYSEDAFSRVAKFHGTEVRYSLGGFRKYPKAIKRRLLRDGLL
                     RMKKNLVDIHHIHIDGIMDLAEGSEVGSSLDLPDNFKARVDYNDLVLYRNHGVREEDL
                     PVPDFEYELKNPGRTHIPQLGMAITLQEVTDFKVLNGDYGCIYIDADSIQGGLYIRNR
                     RDGDRFRPLGMSGTKKLKDFFIDRKVPRHKRDSIPLVVDRNNIIWIAGYQMSEDYKVT
                     HNTKRLIRMEIEED"
     gene            complement(5532..>6812)
                     /locus_tag="VFD89_00160"
     CDS             complement(5532..>6812)
                     /locus_tag="VFD89_00160"
                     /inference="COORDINATES: protein motif:HMM:NF039380.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZJ56641.1"
                     /translation="LYGSCWERNFTRTYNGMFDIIDAIESMGTLEGVNVRGDLYKNCI
                     HLGSLTGSIERVYGLYKSNLKWKTKIDECRQLVAQQLDGVSVVVGQLATELNIDISFN
                     KDLEETISLDLDKAGIRAKEVLVMEKPGGSIEVNIHKKSCSGRRECTRKIEGIVSKAL
                     ARRMSANQPSCIAMGKGECALRLLEAEEFQVSTGIAGKPGKSSEVSGDSYSFNALSHG
                     KYMLAISDGMGIGHKAREESRVAISLLENFLEAGFDLNTTIRSINSTLLLRSQDEIFA
                     TADLCLMDLVSGKADFVKIGAVSTYIKRDNRVEVVKASSLPIGILDSIQPETTSIRLR
                     DEDMIVMITDGILDNSAMGNMAEEWLIGVIGSLETKNPQEVADHILESVLKADNRVIP
                     LKANHEPGGGKPRTGPISPDDMTVMVTRVWKPIL"
BASE COUNT         1641 a         1756 c         1412 g         2003 t
ORIGIN      
        1 ggcgatcagg cgggatccct tgagaatatc ttcttggagc tgacggggct atgagtagat
       61 ttattgcact tttaaaagcg agtctcaacg tccatttcgg tatttcttct ctgaaatacc
      121 gctttactaa ggaaaaaaag cgcctgtggg agcccatcgc cgttctcatc gccatcgtga
      181 tagggggcgg cagcattctt actatgtaca gcctgttgct tctggcggtc tttatggcgg
      241 gccaagccct ggggcatccc gagatagtca ttaccctgtc ctttgtggcg tcgcagctgg
      301 ttatactgat cttcggtatc ttctatatca tatctgtttt ctatttttcc aacgatatgg
      361 gcatactgat ccccatgccc ataaggcccg gaaaggtctt gggcgtaaag tttattacca
      421 tcttgctgag cgagtattta atagcccttc cgatgctttt gcccgccttt atcatatacg
      481 gtgctggttc ctggcagggc atactatatt ggattaaggg attaatctta gtccttacag
      541 cccccatttt gcccctggtt attgcagcat tgtttgttgt ccttttgatg cgctttataa
      601 atattcgaaa gggcaaggat ctcatggtgg taatcggcag cctctttggg ctcatggcgg
      661 ggctggggat aaactttttt acccaaaatc tgccccaggg cagcgaagaa gagtttataa
      721 agcacatgat agagagtaat accgctctta tcgaatccat cggaaataaa tttccgccca
      781 gcatatgggc taccttggcc ctaacccttc ggggatggca tggatgggcc tactttctgc
      841 tcttcgtggg gctttcgttt ctgttggtga tcctcctcta ttggataggc aaccacttct
      901 tctacagggg ttacctgaca ggtcaggaga ttcgccgaaa aaacaaagcc atatcacagc
      961 aggatatgca aaagcgaatc agcagagcat cctcaccttt tctcgccttg cttaaaaggg
     1021 agtggagatt atttatgcgt acccccatct atatgatgaa cggtttggcg ggtatgatta
     1081 tgttccctat ttttttaatc atgccctttt taactaaatc cgatgaattg acggaggcat
     1141 tcggctatgc aaaggatcct gaatatgccc taatagtcac cttggttttt cttggcataa
     1201 tgctgtttgc atccagcatt aatatggtat cctgtacatc catctccaga gagggtgcaa
     1261 ctttttggat ttcgaaggtt atacccgttg cgccgaagga tcagatcctg gccaaactcg
     1321 cccatagcag catcctatcc ctagtaggcc ttataataat ggctataccc ctctacgcat
     1381 tctttaatat ttccttgatc catttgacga tcctgatcat attggggcta ttggccaacg
     1441 ggcttatcaa tatattgggc ctcatggtag atctactgag gccaaagctc tcatggaatg
     1501 accctcagga ggccataaag caaaacctca atgtgttttt tagcttattg gcatcccttc
     1561 tgatcatggc catcctggca gttgcctcta tcgtccttat agtcgcccag gtcaaccaat
     1621 tttgggtcta cgcagtcttg gcccttatca ttatagtact gtcggtgcct gccctatttg
     1681 gattgtttgc tctggctaaa acccgctata agagtataga gatatagacc acttttaaag
     1741 ggatggcttt gaagatatag gtcttttagt ggtaggtctt ttaaaagaaa tgggccctag
     1801 ctttttacaa atcagtgatg aaagcggggc tcaatggttc attacatttt tcatatacta
     1861 tggtgatgga tttgcacaac acgttgggat atgaaacttt tgcatggttt tgatataaaa
     1921 cgactagcta aatatgtcct ttaggagttt atccaaatcc ctgtcgctga gcttggatgc
     1981 ccccgctcta ttgcatacgc tacaatttgc acagttcttg aatttgacgc tttcatcgaa
     2041 gtattctaaa ataaaacgtc ttaggcattt ctttccctca caatattcct ctatctccat
     2101 caatcttccc atctctatct tcttatgcca gtcaatctcc tcctgggtca gatcgaattc
     2161 ccggttaaaa acgggataca cgataccgcc gtttaaccta gcaaagttgt attccatcag
     2221 ctttctcaag gcgctttgta gggtatgcct gtctatcgac ctgctcctta agatgagctc
     2281 ttgggggatg ccctttaaat ccctggactt tatccgcttg tatagcccct cgaccagctt
     2341 aacaggggga taattcccct ctatcatgaa ccgatttaga ctgtaatcgt tttccgagtg
     2401 cagtagaata cagctggatg ccaagccgtc acggccggcg cgccctgcct cctggtaata
     2461 gttttcaagg cttttgggca tattccaatg tatgatatat ctgacatcgc ttttatttat
     2521 gcccataccg aaggcattgg tagccaccat gacctttata tcgttcctta aaaaggcttc
     2581 ctgggcatcg tttctggtaa acctatccaa acccccatgg tacattcccg tggctatacc
     2641 cctccccctc aataagaggt ggatctcctc tacattggct cgggtggccg catagataat
     2701 tccgggccta tgcctgtttt tttggatatg ccttaggata taggtccttt tgtcctcaca
     2761 tttttctacc ttaaattcca aattgggcct gtcaaagctc tcaataaaaa cattgcaatc
     2821 atccttcata cccaggaggt ccgcgatatc gctttgtacc tttccagtag ccgtagcagt
     2881 aaaggcggcc aatatggggt tacctatatg ccctataaga tccctaatct ttagatacgc
     2941 ggggcgaaaa ttatgtcccc attgggatat acaatgggcc tcgtctaccg ccacaaaggg
     3001 aacatgaatc tcctttaatg ccaccaaaaa atcctcggag taaaacctct cgggggcaat
     3061 gtagacaatt ttgtatttgc ccatcttcat atcctcgatg gtctttgtta cctgcctgtc
     3121 cgacaatatg ctgctgatat atgcggcact gatatttagc tccgataact cgtccacttg
     3181 atccttcatt agggatatta ggggcgaaat aacgatggtg gtcccgggga aaataagggc
     3241 gggcagctga tagcagaggg atttcccccc gccagtagga aatatggcga ttgtgttact
     3301 gccttttaaa atactctcga tgacattttt ctggcccgaa agaaagctgt tataaccgaa
     3361 atacttctct agggacctct ccattaccca tatatagccc ataatattcc cactcctcct
     3421 attcccctag tctatccaca tatttgcaga aaatcaagga aaattgtatc aatcgacaaa
     3481 atcaattgga ctatatatgt tacgaaaaag acttttcaat gtaaaatcgg caaaattatc
     3541 cgattttacg ttaaaataat gtattttcgt aacatacgag ttaaggataa tgttttacga
     3601 cacctatgta ccaatgtaga atgtttgtcg taaaacatat agtagcccta tattttacga
     3661 tagatatcct aaaccgagta agattcaggt atcgtaaaac atatagtatc aaaaattata
     3721 ctaaaatagt catatagcaa agattatact aaagcaatta taattctgct ggaaaagcta
     3781 tgggccgtgg ggattaatcc tcctctattt ccatccttat aagcctcttg gtattatggg
     3841 taaccttgta atcctcgctc atctgatagc ccgcaatcca tatgatattg ttcctgtcta
     3901 ccaccaaagg tatgctatcc cttttatggc ggggtacctt tctgtcgata aaaaagtcct
     3961 tcagcttttt ggtacctgac atgccgaggg gcctaaaccg gtccccatcc ctgcggtttc
     4021 tgatatacag cccgccctga atactgtctg catcaatata gatacaccca taatccccat
     4081 ttaaaacctt gaaatctgtc acttcctgaa gggtaattgc catgcccagc tggggtatat
     4141 gggtcctccc cggattcttc agctcatact caaagtccgg gaccggtaaa tcctcttccc
     4201 ttaccccatg gttcctgtag agcacgagat cattgtaatc tacacgagcc ttaaagttgt
     4261 ccggaaggtc caaggaagat cccacttccg aaccttcggc gagatccatg attccgtcta
     4321 tatgtatatg atggatatcc accaggtttt tcttcatacg caaaagcccg tcccgcaaga
     4381 gccttctctt aatcgctttt gggtattttc taaacccccc aaggctatag cggacctctg
     4441 ttccatggaa tttggcgact ctgctaaagg catcctcgga atagctcctt aaaaactctt
     4501 cgtcatccct taggatatcc cccatcctgg ttaagccggc agaaaaattg gggttaaagt
     4561 gctcctctat catgggtatt accatatgcc tgaccctgtt tctggtgtag attgtttcct
     4621 cattggtatg atcgtgtcta aacccaaggc cttccctatg gcaataatcc tcaatctcct
     4681 gccttgaaac cccgagaagg ggtcttataa ttattccatc tctgacggga tccatacccg
     4741 atagccctgc aagacccgac cctcgtatga tgttatagag gatggtttca acctgatcat
     4801 ccctgtgatg gcccagggca atcctttggg ctcctaccct ttgccttacc tcccggtaga
     4861 attcatgcct gacccttcgc ccggcctgct cttccgatac gccccattcc tttaccagat
     4921 ccggtacgga ggcctccttt agaaaaaaat caagccccca ttccgttgcc atggcttcaa
     4981 cgaagagggc atcttctctg gccgcctcgc ctctaatacc gtgatctaca tgggccacat
     5041 acaggttcat ggggataaat tctcgcatcc tccacagaag atggataagg gccacggagt
     5101 ctgcaccgcc ggataccccc accactaccc tgtcgccggg ctccagcata tcgtactctt
     5161 ggatataatc taagactttg tttaacattc tttcagctcc aaatttggct taccttattt
     5221 tattatcttt ccgctattat ttcaagggta ataattaaat agccgctccc cactaaacgc
     5281 ataatggcta ttgatcgttc cttccaacat cgattcggag aaaactaagg cttattccat
     5341 ttgaaaagcc tatcgcttcc aatccggcct cctgccggag gaagcggcct aaggcgtcct
     5401 gccttatgct acggcttttc tgcattccat aagccaagtt ttcttagcca aatcaatgta
     5461 agtcatccac tcttcaatag ctcattatgc ttcttgttac gcatgctgta tcggtaggac
     5521 aaaatttagc gttagaggat cggtttccat accctagtca ccataacggt catatcatcg
     5581 ggagatatgg gaccggtccg gggcttgccc ccaccgggct catggttggc tttcaagggg
     5641 atgacacgat tatccgcctt cagaacggat tctaggatat gatccgccac ctcctgtggg
     5701 tttttggtct ccaaagagcc tatgacccct atcagccact cttccgccat gttccccatg
     5761 gccgaattgt ccaatatccc gtcggttatc atgacgatca tatcctcatc ccgaagcctt
     5821 atggacgtag tctccggttg gatactgtcc aatatcccta tgggcaggct ggatgccttg
     5881 accacctcca ccctattgtc ccgcttaata taggtggaga cggccccgat ctttacaaaa
     5941 tctgcctttc ccgataccag atccatgagg catagatccg ccgtggcgaa tatttcatcc
     6001 tgggatctca ataaaagggt ggaatttatg ctgcggatgg tggtgtttaa atcaaatccc
     6061 gcctccagga aattttctag gagggatatg gcaacccggc tttcctccct ggccttatgg
     6121 cctatcccca taccgtcgct tatggccagc atgtacttcc catgggacaa ggcattgaac
     6181 gaatagctgt cccccgaaac ctcgctggac ttaccaggct ttccggctat cccggtgctg
     6241 acctgaaatt cttcggcctc cagcagcctc agtgcacact cccccttccc catggcgatg
     6301 caagagggtt gattggcgga catcctcctg gccaaagcct tgctgacgat gccctctatc
     6361 tttcgggtgc actctcggcg cccgctacag gactttttat ggatgtttac ctcgatactg
     6421 ccgccgggct tttccatgac aagtacctcc ttggccctga tcccggcctt gtccagatcc
     6481 agggagatgg tctcctccaa atccttgtta aaacttatat ctatatttag ctccgttgcc
     6541 agttgcccta ccacgacgga tacgccgtct agctgctggg cgaccaattg cctgcactcg
     6601 tctatcttcg tcttccactt taggttggac ttgtataggc catagaccct ctcgatggag
     6661 cccgtgagac tgcccaagtg aatacagttt ttatagaggt cccctctgac attgaccccc
     6721 tctaaggtgc ccatggattc tatggcatcg attatgtcga acatgccgtt ataggtcctg
     6781 gtaaagtttc tctcccagca gcttccgtac ag
//
LOCUS       DASRNX010000007        17167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_638495,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000007 DASRNX010000000
VERSION     DASRNX010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17167
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638495"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..457
                     /locus_tag="VFD89_00165"
                     /pseudo
     CDS             356..457
                     /locus_tag="VFD89_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157204.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
     gene            603..1370
                     /locus_tag="VFD89_00170"
     CDS             603..1370
                     /locus_tag="VFD89_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56642.1"
                     /translation="MKSGRIRWGLALCIILGFVIGAMLWFNGLLAAKVYPAFQAFRGY
                     RILQSVPHYEREQVGNFTVRYDSVDFDRFHDLEEIIDEYGGRVLDLFDYAPVKAIDVI
                     LFSDEGEFHQSLGIPMDQPTIGAYSGGKIAVLERSLDGLEENMLINSFVHELTHLVVD
                     NLAGGNFPVWFTEGSALYIEHMLLGYEWGQELGVTDTYSIEELTDDFYGLDEYRAYRQ
                     AFIEVRAMIERIGWDGYLDLLRVLKDGSDPLSQFMKF"
     gene            1494..2816
                     /locus_tag="VFD89_00175"
     CDS             1494..2816
                     /locus_tag="VFD89_00175"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZJ56643.1"
                     /translation="MEKLRIYGGKPLVGRVKISGAKNAAVAILPAALLANDTCTIDNL
                     PYIDDVTTLADILGELGARVELETGGRISINGSKMTEYRAHYDMVRRMRASYYLLGVL
                     LAKFGRAEVSFPGGCAIGTRPIDQHIKGFEALGARVELEHGVIKVFADRLVGNEIYLD
                     VVSVGATINIMLAAVKAEGTTTIVNAAKEPHVVDTANFLNTMGASVMGAGTDTIRIKG
                     VKELSGCDYTIIPDQIEAGTYMIAAAATGGDITVVDVIPKHMEAVSAKLLEMGMEVVE
                     GDDVIRVIGKQRPRKVNIKTQPYPGFPTDLQQPMSALLSVAEGVSVITENVWEARYKH
                     MDEIVRMGGNVKIEDRVAVIEGVERLSGAPVTATDLRAGAALIVAGLLADGITEISNI
                     DHIDRGYEGIEQKLVGLGADIERIDENGKTRRLKLVAPSHGLAAKAVL"
     gene            2937..3734
                     /locus_tag="VFD89_00180"
     CDS             2937..3734
                     /locus_tag="VFD89_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZJ56644.1"
                     /translation="MGLRFCSLFSGSSGNASYIGTGKTHILVDAGLTGKAVERSLKEI
                     DQDASDLDGILVTHEHDDHIRGVGVLSRRYDIPIYANQETWKAMEAKLGKISDNNVRI
                     FYNDMDFYIKDVNIQPFGIPHDAADPVGFCFFNRGKKISIATDLGHTNSRIINTVMDS
                     DLVMLEANHDVEMLMGGSYPGHLKKRILGSKGHLSNTDAGLALVKMITNKLTHVLLAH
                     LSLQNNIPQLAYQTVTEILADSGIENGRDIAVDMTYRDRVSNLYHIG"
     gene            3823..4944
                     /locus_tag="VFD89_00185"
     CDS             3823..4944
                     /locus_tag="VFD89_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZJ56645.1"
                     /translation="MKRNRYLCLNCLLILAILLSAVGCARERPDIQGENLTQNDALRP
                     GSGVERVRARERVLTSGTVVDVAESIIPAVVGISTIEITRESMWGEAMAVKGVGSGVV
                     VHPFGYILTNDHVAGGNPSSINVIFNDGTELEGRTLWTDPTLDLAIVKVDAADELIYA
                     PLGSSDDLAIGETAVAVGTPLGLQFQHTVTSGVISALNRTVKVPTNLGENFMEDLVQT
                     DASINPGNSGGPLVNIAGEVIGINTIKVISAEGIGFAIPIDVAKPVIRHFVEEGGFVT
                     PYLGVVGFDREMASYYNGNSYIDEGIYVVELDKKGPAYSAGMRVDDVITMIGDERVNK
                     MLDLRKAVYSHKVGQSIDVRILRGGQNLTIKMTLVEKPI"
     gene            5341..6333
                     /locus_tag="VFD89_00190"
     CDS             5341..6333
                     /locus_tag="VFD89_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HZJ56646.1"
                     /translation="MVNSIKGSKQPTVLGVNLVYLGVLVLMIVAQAVLVRWLPKATGG
                     MEYYWKLFVMELVLIGGPPMLYMLITRVDISRVNRFNGIKPAEVLLIIGMANFGYGVT
                     IIINLVWFWVASRWGTPAGQELPAIENGVQLLTAVLAIGVVPAVVEEFLFRGLILRGY
                     ERFGAKMAIVMTGILFGMLHLQLMSIPSIILVGIIISYVVYRTNSIIAGMIYHFIHNS
                     IAVVFLFIQNVVLTSQEAIEGIPQDITQLPEEALMAAFVIWGIIGFICLILFVACFIT
                     FHIYTRNKGQVRDVAPGEMEQSKFLGMLPAIIAMIIVIINVVLEVLHMQGIVGG"
     gene            6341..6841
                     /gene="rlmH"
                     /locus_tag="VFD89_00195"
     CDS             6341..6841
                     /gene="rlmH"
                     /locus_tag="VFD89_00195"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HZJ56647.1"
                     /translation="MNIRIVAVGRIKENFLRQEIGMYLGDIGRFCPVEIIEVADERAP
                     QGLSSAEEKAIKAKEGQRILRGIRPNSYTIALAIEGQALSSRVFLGRLYDISLNIKTN
                     IDFIIGGSLGLDEGILKRADFLLSFSRLTFPHQLMRLILLEQIYKGVSGKMGNPGYMD
                     TGPRHR"
     gene            7202..8170
                     /locus_tag="VFD89_00200"
     CDS             7202..8170
                     /locus_tag="VFD89_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZJ56648.1"
                     /translation="MEVKKLFAILVVLVVFVGLTGCTNSAKGPKVGIIQLAEHVALDS
                     ASEGFIAALEDGGYVDGENITIDLQNAQGEQTNLSTIGDRFVSEKVDLILAVATPAAQ
                     SIAGKTSTIPILGTAITDYVDARLALSNEEPGLNVSGTTDMNPIKEQIELLIKLAPET
                     KTVGVIYNSSEDNSILQAKIAKENIEGMGLNYEEVTVTSSNDVQQATQSLVEKCDAIY
                     IPTDNTLATAMPVVHGVVVKSKTPMVCGEESMVREGGLATIGINYYDLGYQTGLMAME
                     ILKDKADISKMPIKSATKFDFAINGNVAEEIGIAIPDDLTEHIIDE"
     gene            8254..9156
                     /locus_tag="VFD89_00205"
     CDS             8254..9156
                     /locus_tag="VFD89_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZJ56649.1"
                     /translation="MFEVIKGALSLGLLWGIMTIGVYITYRILSIADLTVEGSITLGA
                     AIAAHAITRGMNPYLGTLLALFGGMAAGFCTGILHTRLKIPALLSGILTMIALYSINL
                     RVMGKANVSLLRMDNVYTVFENLGLEPTWAIITLGAIGVVVIVCLLYWFFGTEIGCAI
                     RATGNNPYMVRAQGINTNSMIILGLVISNGLVSLGGALIAQNQNFSDIGMGTGSIVIG
                     LASVIIGEVLFGKRNFFMRLISLAFGAITYRIIIAFVLKLGMHSNDLKLFTAITVAVA
                     LALPMIKDLLNPPKIVIGEGGQDA"
     gene            9149..9943
                     /locus_tag="VFD89_00210"
     CDS             9149..9943
                     /locus_tag="VFD89_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZJ56650.1"
                     /translation="MLRIDGAYKIFNPGTVNRKMALRNVSLSMKEGDFITLIGGNGAG
                     KSTLLNCIAGVYGVEKGSINIAGIDVTKSSEYKRAALLGRVFQDPMMGTASDMILEEN
                     LALALRRGKPRGLRWGISNKERETYRELLSHLGLGLEKRLNVKVKLLSGGQRQAITLL
                     MATLKKPKLLLLDEHTAALDPKTAGIVLELTERLVHENGLTTLMVTHNMRDAIRYGNR
                     LIMMHDGRIFLDIQGEEKSKLTVEELLTRFGSASGQEFSYDRALLS"
     gene            10259..10582
                     /locus_tag="VFD89_00215"
     CDS             10259..10582
                     /locus_tag="VFD89_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HZJ56651.1"
                     /translation="MDKKGFYERVYKIVGKIPEGKVMTYGLIGFLLGSRYYARRVGSA
                     LANAHGRGLPCHRVVNSKGELAPPHVFGGVGNQYRILKGEGVFFKSNGSVDLKRSLWR
                     TWDGV"
     gene            10758..12005
                     /locus_tag="VFD89_00220"
     CDS             10758..12005
                     /locus_tag="VFD89_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZJ56652.1"
                     /translation="MVRKSMGDFIAVLRKAKGLTQAELADKLSVSDKTVSRWERGEGA
                     PDISLIPIIAHIFGVTSDELLRGERNPTAPGDLDFSGELSKRGQKQFTRLLEMNLSKA
                     KNQSLVSLGIMVTGLIGAMICNFGYNRGYLGFFVSALFSMTGILYGIIISNMALLKVS
                     RDGFDGEELKRYKRSILLMVRGILILGVILSATSLPLILTGDPLFGVMGRSWLRLGLL
                     YGIIALALCLIIVDLWDWILIRKGIYILGERENDTYRFNLDLKKRYGLILLGIFLITL
                     GGHWFLDRKFGINEGDFLAHNLYAENAYGMEYDDGSVDYLDEYGNTIYEEGLPRENSG
                     GDEGGVLYTYVKRLGNAAGPIIYSGKRGFFPFTIYNYRAYSFGSVPWGKIIMIIPNIG
                     FFLVYLVEILAIIAIYYRKRGRE"
     gene            12268..13179
                     /locus_tag="VFD89_00225"
     CDS             12268..13179
                     /locus_tag="VFD89_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HZJ56653.1"
                     /translation="MIYPKLSDEFLIKRLEPPTGRVRAVLDTDTYNEVDDQFALAYAL
                     RSDDKIDLEAVYAAPFHNDRSEGPGDGMERSYQEIKNVLRLLDTPMESVFRGSTDYLK
                     NLDKPHKNEAVTDIIDRAMASDHDEPLYVMAIGAITNVASAILIEPEIIKKIVVVWLG
                     GHAHYWPDTKEFNLAQDINATRTIFDSGVPLVQIPCMGVTTHLHTNISELEYYLDGKS
                     QIGTYLTDIVRNYTNNPYAWSKVIWDISAVAWLINPDWVPTNLTHSPMVTDQVTWSFD
                     VSRHLIRCANFIHRDPIYGDLFKKLSK"
     gene            13207..14145
                     /gene="rbsK"
                     /locus_tag="VFD89_00230"
     CDS             13207..14145
                     /gene="rbsK"
                     /locus_tag="VFD89_00230"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HZJ56654.1"
                     /translation="MEGGAVRFLVIGSINMDLVMELQTVPGAGETVLGRGYSYIPGGK
                     GANQAVAAARLGGEVAFCGRVGNDSNGKILLDSLRADNIDTSFIKMDDKHPTGLAVIP
                     VESNGENRIMVFPGANDHITRGDIDRVMEQAYDAIIMQLEIPLDVVYYAFDKAKAKGI
                     PVILDAGPARAICLSKLQGIHVLSPNESEATALTGIKVEGWESSLEAAKMLANASGAS
                     YVVIKMGEKGALLYERDRNAHEVFPAYRVKAVDSTAAGDSFTAAMTVKMLEYGDIRRA
                     IPYANAVGALCVSKIGAQPSLPGADEVEQFLALRDR"
     gene            14142..14540
                     /locus_tag="VFD89_00235"
     CDS             14142..14540
                     /locus_tag="VFD89_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HZJ56655.1"
                     /translation="MKIKIISKNKIKGGFAGEAIEEYTKRLARYCRTELKFTHNPEKA
                     IKDRDYVIKVGKGGAVLSSEQLAERISSLGIGGNSDIAIVLHDDCKGLRIDFSLEISR
                     MDIHEDLMLIMLYEQIYRAYRIINNEPYHK"
     gene            14577..15200
                     /locus_tag="VFD89_00240"
     CDS             14577..15200
                     /locus_tag="VFD89_00240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZJ56656.1"
                     /translation="MGERLLDRALKVRTTGIREWHRGEVHYNRYEATPYTALDKLFET
                     YSLEKTDELVDFGCGRGRVIFYIHNRFEIPVTGIEANDKTYEEALGNKGAYRHRAKHI
                     KAPIRLEYGPAEDYEIKSTENRFYFFNPFSIKIFRKVVNNILASVAENPRSVDLIIYY
                     PTALYRQFLKKNTLFEIINKAKTPGMTDKKEKFLVYRLRQDKPDGIV"
     gene            15268..16092
                     /locus_tag="VFD89_00245"
     CDS             15268..16092
                     /locus_tag="VFD89_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZJ56657.1"
                     /translation="MKITFNGTGASEGFPALFCECEHCTEVRTMDPINFRMRSSCLID
                     ESLLVDFSSDTYARCLYGKLDLTQVKDIIFTHSHSDHFYPEDLGKIAPPFGLHNRKEP
                     LRIYGNRNIGQALEDMGMTGPDKSPYLRFSPMVAFETYHIANYKVTPLPANHDPSQEC
                     FIYVIQGDDKTLLYGHDSGYFGEETWAGLKNFKFDGVILDCTAVDEECPYPSHMGLPD
                     NIRVRERMYNGGAADGQTTFIITHFAHTNGPFYEYLNGLARETGFIAAYDGLEIIL"
     gene            complement(16266..16940)
                     /locus_tag="VFD89_00250"
     CDS             complement(16266..16940)
                     /locus_tag="VFD89_00250"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZJ56658.1"
                     /translation="MQILKNDWQDHVEDEFNKDYYLALRQFLAAEYRTKTVYPDMYDI
                     YNALHYTPYKDVKAVILGQDPYHGPNQAHGLSFSVKPGVQTPPSLLNIYKELKDDLGC
                     YIPNNGYLEKWARQGVMLLNTVLTVRAGQANSHKNMGWEHFTDRVISILNQREDPVVF
                     ILWGRNAQSKQSIITDPQHYIIKSPHPSPLSAHRGFFGSRPFSRANDFLASTGREPID
                     WQIENI"
     gene            complement(16959..>17167)
                     /locus_tag="VFD89_00255"
     CDS             complement(16959..>17167)
                     /locus_tag="VFD89_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56659.1"
                     /translation="IAVGTIRLQRGGKFLPAARFDYFYNLILDIGNNFVNYGIIHPYN
                     LEEFDNVLNKKISMACDTLRGYPA"
BASE COUNT         4798 a         3228 c         4491 g         4650 t
ORIGIN      
        1 taaaacttgt gtaacaattt tggggattct atgatataat aaatgcatcg gtgtgaagca
       61 agccaattaa ggcttgcacc tattaaaaga tagtatcata ttctaatgtt gccctatagc
      121 cgacgaggtt tgggtgtatc ggggcgggaa ggacacttaa taatgttgca aaagatccga
      181 ctacaggtat tggacaaggt gtttaactga atggggctaa ggaaaagtat tgtcatagcc
      241 ttataagtta tttttttatc agtgcgggaa tattttctgt taaggtgctg gctacaaaca
      301 atgaatcatc cccgaaagat gatatggtag aggcattttt tacatatctc gattcatcgg
      361 ctatatgggt agcaccggta atagcacagg ttcacacctc cacttcgaaa tccccgctag
      421 cggaagcccc aagaatccgc ttaactatct aaaataggtc ccatgtttca aaccccataa
      481 gggggctcac ctgtctcccc tttagattcc gggaaactat gccattaatt gcttgatgaa
      541 gtttgcataa taagatgcta taatgggttt gctgtgccgc tcgggtgagg gaggtacaac
      601 cggtgaaaag cgggaggatt agatgggggt tagcattatg cataatcttg ggctttgtga
      661 taggagcaat gctttggttt aacggccttt tagcagccaa ggtctatccc gcattccaag
      721 cctttagggg ctacaggatt ttgcagtctg taccccatta tgaaagggaa caagttggca
      781 attttactgt acgatatgat tctgttgact ttgatagatt tcatgacctg gaggaaatca
      841 tagacgaata tgggggtagg gtattggacc tctttgatta cgctcccgta aaggctatcg
      901 acgtgatttt gtttagtgat gagggggaat tccatcaaag tctgggcata cctatggatc
      961 agccgactat aggggcatat tcgggtggaa agatcgctgt tttggagcga tcgcttgacg
     1021 gacttgaaga aaatatgcta atcaacagct ttgtgcatga attaacccat cttgtagtag
     1081 acaatttagc cgggggtaat tttcctgtat ggtttacgga aggcagtgcc ctttatatag
     1141 agcatatgct attgggctat gaatggggac aggagcttgg agtcacggat acctattcga
     1201 ttgaggaact gactgatgat ttttacggat tggacgaata ccgggcatac aggcaagctt
     1261 ttatagaggt cagagccatg attgaaagga ttggatggga cggttattta gatctgcttc
     1321 gggttttaaa agacggcagc gaccctttat cacaattcat gaaattttaa aacttttttg
     1381 atgaaatact attgtaaagg tgttataata aaaacattgc tttgtatgga cgagacataa
     1441 tggcattgga ggtgaagttc cctcaggaaa cagagaaaag ggggaaccag ggattggaaa
     1501 aactacgaat ttatggtggg aagccattag ttggaagagt gaaaataagc ggtgctaaaa
     1561 atgctgctgt tgctatttta cccgcagcgt tattagccaa tgatacctgt actatcgata
     1621 atttgcctta tatagatgat gttactactc tggccgatat tttaggggaa ttaggtgcac
     1681 gggttgaact ggaaaccggg ggaaggataa gtattaacgg ctcaaaaatg acggaatata
     1741 gagcccatta cgatatggtg aggcgaatga gagcctctta ttatctttta ggtgttttgc
     1801 tggccaagtt tggcagggca gaggtatcct ttcccggcgg atgtgccata ggtaccagac
     1861 cgatagatca gcacatcaag gggttcgaag ccttgggagc cagggtggaa ttagaacatg
     1921 gcgttataaa ggtattcgct gataggttgg taggaaatga aatttatctc gacgtggtca
     1981 gtgtaggtgc tacgataaac ataatgctgg cagctgtaaa ggcagaggga accactacta
     2041 ttgtcaatgc tgccaaggag ccccatgtag tggatactgc aaatttctta aataccatgg
     2101 gagcaagcgt tatgggtgcg ggtactgaca ccatcaggat taagggtgta aaggagcttt
     2161 ctggttgcga ctacaccatt atcccggatc aaattgaagc cggcacctat atgatcgccg
     2221 ctgctgctac gggaggggat attacggtgg tagacgttat tcccaagcat atggaggcgg
     2281 tttctgcaaa attgctggag atgggtatgg aagtcgtcga aggggatgat gtgattcggg
     2341 tcataggcaa acagagaccc cgcaaggtga acataaagac ccagccatat cccggatttc
     2401 ccacggattt gcaacagccc atgtctgcgc ttctcagcgt tgcggaaggg gtcagcgtaa
     2461 ttacagagaa cgtatgggaa gccaggtaca agcatatgga tgagattgtt agaatgggcg
     2521 gtaatgtaaa gatagaggac agggtagctg tcatcgaagg cgttgaaaga ttatccgggg
     2581 cccccgtaac agcaacggat ctacgtgcag gtgcggccct tattgtagcg ggcctattgg
     2641 ctgatggaat taccgaaatc agcaatatag atcatatcga caggggttac gagggaatag
     2701 agcaaaagtt ggtaggtttg ggtgcggata tcgagcgaat agatgaaaac ggcaaaaccc
     2761 gcagactaaa attagtggcc ccaagccatg gattggcagc gaaggcggtt ctttagggcc
     2821 ggacataggg aatagatata tttatttttg ctattcataa aatttaagtt aatttggagg
     2881 gggatgaccg gtggttatcc ccctttttaa ggaatgtttt gttggagggg atttaaatgg
     2941 gattgaggtt ctgctccctt tttagcggga gtagcggcaa cgccagttat attgggacgg
     3001 gcaagaccca cattctggta gatgccggtc ttaccggaaa ggcggtcgaa agatcattaa
     3061 aggaaataga tcaggatgcc tccgacttgg atggaatctt ggttacccat gaacacgatg
     3121 atcatataag gggcgtgggg gttttatcta ggagatatga tattcccata tatgccaacc
     3181 aggagacctg gaaggcaatg gaagcaaagc tggggaagat cagcgataac aatgtaagaa
     3241 tcttttataa cgatatggac ttttatatca aggatgtgaa tatccagccc tttgggatac
     3301 cccatgacgc ggcggaccca gtgggatttt gcttttttaa taggggtaaa aagattagta
     3361 tagcaacgga tttagggcat actaactcca gaattataaa tacagtcatg gattcagacc
     3421 tggttatgtt agaggccaat catgatgtgg agatgctcat ggggggatcc taccccgggc
     3481 atctaaagaa gcgtattttg ggaagcaagg gacatctttc aaacaccgat gccgggttag
     3541 ccctggttaa gatgataacg aataaactta cccatgttct tttggcccat ctcagccttc
     3601 aaaataatat accccagttg gcataccaaa cggttaccga aatattagcg gacagcggta
     3661 tcgaaaacgg gagggatatt gcggtggata tgacctatag ggaccgggta agtaatctgt
     3721 atcatatcgg ttgatagact agtaacaaac acatcaactt tccttaaaga gctggattct
     3781 aggtcaattg tccatgactt gagggagtga atatgtccga ctatgaaaag gaatcgttat
     3841 ttgtgcctta actgcctatt aattcttgca atattgctta gcgcagtagg gtgtgcccgg
     3901 gagaggcccg acattcaagg ggaaaatttg actcagaacg atgctttaag gccaggatcc
     3961 ggggtggagc gggtcagggc acgggaaagg gtgttaacca gcggtacagt agtggatgtg
     4021 gctgaaagca ttatccctgc ggtggtgggg atctccacta tagagatcac tcgcgaatcc
     4081 atgtggggtg aggccatggc cgtaaagggc gtagggtccg gtgtagtggt gcatcccttt
     4141 ggctacatat tgaccaacga tcacgtagcg ggcggtaatc ccagcagcat taacgttatc
     4201 tttaacgatg gaacggaact tgagggtaga accctatgga cagatcccac tttggatctg
     4261 gctattgtga aagtcgatgc tgcggatgag ctcatctatg ctccgttggg gagcagtgat
     4321 gatctggcta taggagagac tgccgtggct gtgggtacgc cccttgggct tcagtttcaa
     4381 catacggtta cctccggagt aataagcgct ttgaatagga ccgtgaaggt ccctaccaat
     4441 ctgggagaaa actttatgga ggatcttgtt cagaccgatg catccatcaa cccaggcaac
     4501 agcggcggtc ccttggtaaa tatagctggt gaagtgatag ggatcaacac catcaaggtc
     4561 atcagcgcag aggggattgg ctttgctata cccatcgatg tcgcaaaacc ggttatcaga
     4621 cactttgtag aggagggggg gtttgtgacc ccttatctag gagttgtcgg attcgatagg
     4681 gagatggcat cttattataa tgggaatagc tatatcgatg agggcatcta tgtggtagag
     4741 ctggataaga agggaccggc ctatagcgca ggcatgaggg tagacgatgt tataaccatg
     4801 ataggcgatg aaagggtaaa taaaatgttg gatctcagaa aggccgtata ttcccataag
     4861 gtagggcaaa gcatagatgt cagaatcctc aggggtggtc agaatttaac gattaagatg
     4921 actttagttg aaaagccgat ataacggggc ggaaagatgt cccccgcttt tgtaagtggc
     4981 gagttcccat tagtagaaca ggggcagtgg gtataagtct cccgccccgt tgaaacctgt
     5041 gtgttgcaat tgctaatgca attgcttccg atgttataac ggataaattt tttggataat
     5101 ccgccggaaa ggcggattat tttgatttta tcccctttcg ggctatattt tgtacgtcct
     5161 tcatattata atgggggtag tgatttcaat ccatcggaag tataggcatg atgtttactg
     5221 aaatcatcga ctttttgctt ataatcttgt gtggggaaaa tccaatcgtg tgttttacta
     5281 tccccgggac ggcggatttt cttagaacac tccggtttgg atagcggaaa ggggaagagt
     5341 atggtaaatt caataaaggg ttcaaaacag cccactgttt taggcgtcaa tttggtatat
     5401 ctgggtgtac tggtgcttat gatagtcgcc caggcagtgc ttgtaaggtg gttgcccaaa
     5461 gcgacgggcg gaatggaata ttactggaaa ttgtttgtta tggaactggt gcttataggc
     5521 ggaccgccca tgttatacat gctgatcacc agggttgata tctcaagggt caatcgattt
     5581 aatggaatca agccggcaga ggtgctcttg attatcggca tggctaactt cggttatgga
     5641 gtaactatta ttataaacct tgtatggttt tgggtggcaa gtcgctgggg aacacctgct
     5701 ggtcaagaac tccctgccat agagaatggg gttcagctgt tgacggctgt actggcaatc
     5761 ggcgtggtgc cggcggtggt tgaagaattt ttgttcaggg gtcttatcct taggggatat
     5821 gaaaggtttg gcgccaaaat ggctatagtt atgaccggaa ttttgtttgg gatgctccac
     5881 ctgcaactta tgagcatacc gtcaattatc ttagtgggaa taattataag ctatgtggta
     5941 taccgcacca attcaattat agccggcatg atatatcact ttatccataa tagtatagcg
     6001 gtggtttttt tatttataca aaatgttgtg ctgacaagtc aagaagccat tgaaggaata
     6061 ccccaagaca ttacccagct tcctgaggag gcccttatgg cggcttttgt catatggggc
     6121 ataattgggt ttatctgtct aatcctattc gttgcctgct tcattacatt tcatatatac
     6181 acccgaaaca aggggcaggt ccgggatgtc gcacccggag aaatggaaca atcaaagttt
     6241 cttggcatgt tacccgccat catagccatg atcatagtta taataaatgt ggtcctcgag
     6301 gtgttacata tgcaagggat tgtgggtggc taggtgtcgt atgaatatac ggattgtagc
     6361 ggtaggaagg atcaaagaga attttcttcg gcaggaaatt gggatgtatt taggggatat
     6421 aggacgtttt tgtcctgttg aaattatcga agtagcagat gaaagggccc cccaaggcct
     6481 atcttcggcg gaggagaagg cgattaaggc caaggaaggc cagcggattc ttagggggat
     6541 cagacctaat tcatatacca tcgctttggc catagaaggt caggctttat cttcccgggt
     6601 ttttctgggg aggctatatg acatatcctt aaatataaaa actaatatag actttataat
     6661 aggtggttca ttgggactgg atgagggtat attaaaaagg gcagattttt tgttatcctt
     6721 ttccaggctt acctttcccc atcaattgat gcggttgata ttactcgaac aaatatataa
     6781 gggggtatcc gggaaaatgg gaaaccccgg atatatggat acgggtccgc ggcatcgttg
     6841 aagtatgtgt attgtaatcg cggcagtgat tgccccataa gtcctcttcc aaatttgccc
     6901 ttacaggata tacggtataa ttattgacaa ccttgtgtaa atatatataa ttaaattaat
     6961 aagaaggtag cagaaggtat gccatccatg gatgatggta tgatgcccgt aattttaatc
     7021 ctattaattt tttgtatgtc ggatattttc tgacagcctt taaaacagtt gagttttccc
     7081 caagggctta ggccttgggg ggagtaatta tagataaaca acttcaacta taaaatttat
     7141 cacatataga taaatttagt tggtctattg tttatctaca aataaaaggt agcatggagg
     7201 tatggaagtg aaaaagttat tcgcgatctt ggtagttttg gtagtatttg taggattgac
     7261 aggttgcacc aatagtgcaa agggccctaa agtagggatt attcaactgg cggagcatgt
     7321 ggcattggat tcggccagcg aaggatttat cgccgctcta gaggacggcg gctatgttga
     7381 tggcgagaac atcaccatag accttcaaaa tgctcagggg gagcaaacaa acctctccac
     7441 cataggcgac cgctttgtca gcgagaaggt tgaccttata ctggcggtag caacgcctgc
     7501 agcccaatcc attgcgggga agacgtccac cattccaata ttgggcaccg ctataaccga
     7561 ttatgtggat gcaagacttg ctctgtccaa cgaagagccc ggccttaatg tatcggggac
     7621 caccgacatg aatcccatca aggagcagat agaactcctg attaaattgg ctcccgaaac
     7681 taaaaccgtt ggagtaatat ataattccag cgaagataat tcgattctcc aggctaagat
     7741 agccaaggag aacatcgagg gaatgggact taattacgaa gaggtgaccg tcacaagctc
     7801 caacgatgta cagcaggcaa cacaatccct tgtggaaaag tgcgatgcca tctatatccc
     7861 caccgataat acccttgcta cggcaatgcc ggtagtgcat ggagtagttg tgaaatctaa
     7921 gacgcctatg gtctgcggtg aagagagtat ggtccgcgag ggtggcctgg caaccatagg
     7981 gatcaactac tatgatttgg gatatcagac ggggctgatg gctatggaga tattaaagga
     8041 taaagccgac atttcaaaaa tgcccataaa atctgccact aaattcgatt ttgccataaa
     8101 cgggaatgta gccgaggaaa tcgggatagc gatcccggac gatttaaccg agcatataat
     8161 cgacgaataa tatagatcat catgattgtt caggggataa tcgattcaaa agtttagtca
     8221 atataggatt ggtctttaga atggagaata tcaatgtttg aggttatcaa gggggcttta
     8281 tctctagggc ttttgtgggg gataatgacc ataggagttt atataacata tcgtattttg
     8341 tctatagcgg atctgactgt agaggggagt attacccttg gggcggccat tgccgcccat
     8401 gccataacaa gggggatgaa tccttaccta ggaacccttt tggctctatt tgggggcatg
     8461 gctgccggat tttgcaccgg gattttgcat acaagactta aaatccctgc ccttttatca
     8521 gggatcctaa ccatgattgc cctatactcc attaacctca gagttatggg taaagcaaat
     8581 gtgtcacttt tgagaatgga taatgtctac accgtatttg agaacctggg attagagcca
     8641 acatgggcca taatcacctt gggtgctata ggagtggttg taatcgtatg cctgttgtat
     8701 tggttttttg gaaccgagat agggtgtgcg ataagggcta ccggcaataa cccctacatg
     8761 gtcagagccc agggaataaa tacgaacagt atgataattt taggcctggt aatcagcaat
     8821 ggtctggtct cattgggtgg ggcgctaatt gcccaaaatc agaatttttc ggacattgga
     8881 atggggacgg gatccatcgt aataggcctg gcatcggtca tcatcggaga agtgctcttt
     8941 ggaaagcgaa acttttttat gaggcttata tcccttgcct ttggtgctat tacctatcgt
     9001 ataataatcg cctttgtctt aaagctgggt atgcatagca acgatttaaa gctcttcact
     9061 gcgataacag tagccgttgc tctggccctg cctatgatca aggacctttt aaacccgccc
     9121 aaaatagtga taggggaggg tggacaggat gcttaggata gacggggctt ataagatatt
     9181 caatcccgga acggtgaatc gaaaaatggc actcagaaat gtgagcctta gcatgaaaga
     9241 aggggacttc atcaccctta tagggggtaa cggtgcaggt aaatcgaccc ttttaaactg
     9301 tattgccgga gtgtatggcg ttgagaaggg gagcataaat attgcgggca tcgatgtaac
     9361 gaaatcatcc gaatacaaac gggcggccct tttagggagg gtatttcaag accccatgat
     9421 ggggaccgcc tctgatatga tcctggaaga gaatttggcc ctggccctga ggaggggaaa
     9481 accccgagga cttaggtggg ggattagcaa taaggaacgg gaaacctata gggaattgtt
     9541 gtcccatctg ggtttggggc tggaaaagcg attaaatgta aaggtaaagc tcctctctgg
     9601 agggcagagg caggccataa cactgcttat ggcaaccctt aaaaagccaa agctgcttct
     9661 tttagatgaa cacacggcag ccctggatcc taagaccgcc ggcatcgtcc ttgaattaac
     9721 ggagcgcttg gtccatgaaa atggtttaac caccctgatg gttacccata atatgcgaga
     9781 tgccatcaga tacggtaacc gtttaattat gatgcacgac ggcaggatat ttttggatat
     9841 ccaaggcgag gagaagagca aattaacagt ggaagagctt ctgacccgat ttggttctgc
     9901 aagcgggcaa gaattttcct atgatagggc attgttatct tagggtctat gtgatttgcg
     9961 atggaagtta ttcgtcttca aataagttca ttagctttgt catatagtga attaattaac
    10021 tgatataccg tattaaatca acatgttaag taagttacgc atatatcggt aatggccatt
    10081 gaacgctatt ttccaaatcg atgaaatcat ttgcttttca atggcacatt acctttgctg
    10141 tatagttaat gtgttggtat aaagcattaa attgtgtaaa tgaaaccggt ggggcggatg
    10201 caggtagatt tagtttgtgt attgtttgtc tataaatata tgttgatgga aatgtgagat
    10261 ggataaaaaa ggattctatg aacgtgtcta taagatagtc ggtaaaatcc cggaaggtaa
    10321 ggtcatgacc tacggcctta tcggtttttt gctgggctca agatactatg ctagaagggt
    10381 agggagtgct cttgctaatg cccatggtag gggtttgcca tgccaccggg tcgtcaattc
    10441 caagggcgag ttagcgccgc ctcatgtttt cgggggagtg ggaaaccaat acagaatcct
    10501 aaaaggggag ggggttttct ttaaatccaa cggttccgtg gatctcaaaa gatccctttg
    10561 gaggacttgg gatggggtat aagtaaatta tccccgtatt tccactgtta caaagccata
    10621 ttctcagaat ttgagagggg ttcttaacct gctgggtgga tttttcacta tcggattgtt
    10681 agaatttagt tatccagatg cggttaggac taaatgatcg cggtttggat aatttctgag
    10741 aatacggagg ttttataatg gttagaaaat ccatgggtga ttttatagcg gtactgagaa
    10801 aggcgaaggg gttgacccaa gcggagctgg cagataaatt gagcgtatcg gataagactg
    10861 taagccgatg ggagaggggt gagggggccc cggatatttc cctaatccct ataattgccc
    10921 atatatttgg agtgaccagc gatgaacttt tgcggggaga gcgtaacccc actgccccgg
    10981 gtgatttaga ctttagcggt gaattgagca aaaggggcca gaagcaattt acaaggttgc
    11041 ttgagatgaa tctatccaag gccaagaatc aaagtttggt ctcccttggg attatggtta
    11101 cgggcctcat cggggcgatg atttgtaact tcggatataa cagggggtac cttggttttt
    11161 tcgtatctgc tctattttct atgacgggaa tcctatatgg gattatcatt tccaatatgg
    11221 cccttttaaa ggtctcccgt gacggctttg acggggagga gctaaaaaga tataaacgat
    11281 ccattttact tatggtcagg ggaatcctta ttttgggggt gatcctttct gctaccagtt
    11341 taccccttat tttaacgggg gatccgcttt tcggggtgat ggggcgatcc tggctcaggc
    11401 tggggctctt atacgggata attgcattgg ccctgtgcct tataattgtt gatctatggg
    11461 attggatttt aatcagaaaa gggatataca tccttgggga aagggaaaat gacacctacc
    11521 ggtttaattt ggatttaaag aaaaggtatg gtcttatcct cttaggcatt tttttaatca
    11581 ccttaggggg ccattggttt ttagatcgaa aattcgggat aaatgaaggg gattttctgg
    11641 cacataacct ttatgcagag aacgcatacg gcatggaata tgatgatggg tccgttgact
    11701 atttagatga gtatggaaac actatttatg aagaggggct gccaagggaa aattccggtg
    11761 gggacgaagg tggagtcctc tatacctatg ttaagcggct agggaatgct gcagggccta
    11821 tcatatatag cgggaagcgc gggtttttcc cctttaccat atataactac agggcatata
    11881 gcttcggaag cgtaccatgg ggcaagatca tcatgataat ccccaatatc gggtttttcc
    11941 tcgtatattt ggttgaaata ttagctatta tagcgatata ctataggaaa aggggcaggg
    12001 aatgataacg ggacgggaaa atgatcccta tttttataag tgatgagatc cgattataga
    12061 taaacaattc caactataaa atttatcgtc acgaaattgc atatacctat tgcaattatc
    12121 atgccgataa acttaatttg cctattgttt atgtacggtt aagacaatcg gatggcatat
    12181 attcaccgcc ctgttgaaat ccgcgtgcag ctatcaccaa cgcaattgct tcctatgtta
    12241 taattacatt aaaaggggtg gattatattg atttatccga aactttctga tgaattttta
    12301 attaagagat tggaaccgcc gacgggcagg gtaagggcgg tattggatac cgatacctac
    12361 aacgaagtgg acgatcagtt cgctttggcc tatgccttga gatccgatga taagatagac
    12421 ctcgaggccg tatatgcggc tcctttccat aacgacaggt ccgagggtcc cggggacggt
    12481 atggaacgca gctatcagga gatcaagaat gtcctcaggc ttttagatac tcccatggaa
    12541 tccgtattcc gtggttcaac ggattattta aagaacctgg ataaaccgca taaaaatgag
    12601 gccgtaacgg atattataga cagggctatg gcatcagacc atgatgaacc cctgtacgta
    12661 atggccatag gggccattac caacgtggct tccgccatcc ttatagagcc ggaaattata
    12721 aaaaagattg tcgttgtgtg gctgggtggg catgcgcatt actggcccga taccaaggaa
    12781 ttcaatttgg cacaggatat aaacgccacc aggacaatct tcgacagcgg cgttcccttg
    12841 gttcaaatac cctgcatggg ggtcactact cacctgcaca ccaatattag tgagctggag
    12901 tattatcttg acggtaagag ccaaattggt acttatttaa ccgatatagt aagaaattat
    12961 actaataatc catatgcatg gtccaaggta atctgggata tctcggctgt tgcatggttg
    13021 atcaatcccg actgggtgcc cactaactta acccatagcc caatggttac ggatcaggtt
    13081 acctggagct ttgatgtgtc tcggcatctg ataaggtgcg cgaattttat ccatagggat
    13141 cccatatacg gggacctgtt taaaaagctt tcaaagtaga attattgtaa agagggggga
    13201 tatgtcgtgg agggtggggc tgtaaggttt ttagtcatag gcagcatcaa tatggatctg
    13261 gttatggagc ttcaaacggt acccggggca ggggaaaccg ttttgggaag gggctattcc
    13321 tatatacccg ggggtaaagg tgcgaaccag gccgtcgctg cagccaggct gggtggggag
    13381 gttgcctttt gcggacgggt cgggaatgat tccaatggga agattttgct ggacagtctg
    13441 agagctgata atatagacac ttcttttatt aaaatggatg acaagcaccc aaccggactt
    13501 gcagttatac cagttgaatc caacggggag aaccgcatta tggtttttcc gggggctaac
    13561 gatcatatca cccgtgggga tatcgacagg gttatggaac aggcctatga cgcaataatt
    13621 atgcaattgg aaataccatt ggacgtagta tattatgcgt ttgataaagc taaagccaaa
    13681 ggcatacccg ttattttgga tgccggaccg gccagggcca tctgcctttc aaaacttcaa
    13741 ggaatccatg ttttaagccc caatgaaagc gaggcaacgg ctcttacggg aattaaggtt
    13801 gaaggatggg agagcagcct tgaggcggct aaaatgctgg cgaacgccag tggtgcatcc
    13861 tatgttgtga taaagatggg cgaaaagggc gcattgcttt acgaaaggga tagaaatgcc
    13921 catgaagttt ttcctgcata cagggttaag gccgtggatt cgacggccgc tggagactcc
    13981 tttaccgcgg caatgaccgt caagatgttg gaatacgggg atataagaag ggccatacca
    14041 tatgccaatg cagtaggggc cctatgcgtc agcaagatag gggcgcagcc ctccctgccc
    14101 ggggcagatg aggtagaaca atttctggct ttgagggaca gatgaaaata aagataataa
    14161 gcaaaaataa aattaaaggt ggatttgccg gagaggccat cgaggagtat accaagaggt
    14221 tagccaggta ttgtaggaca gaattaaaat ttacccacaa tccggagaag gctataaagg
    14281 acagggacta tgtgattaaa gtgggcaaag gcggagcggt tctctcttcg gagcagctgg
    14341 ctgaaaggat atcttcctta gggataggcg ggaacagtga tatagccatt gtccttcatg
    14401 atgactgcaa ggggctgagg atagactttt cccttgaaat atcccggatg gatattcacg
    14461 aggatttgat gttgataatg ctatacgaac agatatacag ggcctatcgt attataaata
    14521 atgaaccata tcacaaataa gaaggtggaa atcaaaaaat aattgggggt atcttaatgg
    14581 gcgaaaggct attggacagg gcactaaagg ttaggactac agggatcaga gaatggcata
    14641 ggggcgaggt acactataat cgctatgagg ctacacctta cacagccttg gataaactct
    14701 ttgagacgta tagtctggaa aagacggatg agcttgtgga ttttgggtgc ggaaggggcc
    14761 gcgtgatctt ctatatccac aacaggttcg agatcccggt aacggggata gaagccaacg
    14821 ataagactta tgaagaagcg ctgggtaata aaggtgccta taggcacagg gccaagcata
    14881 taaaggcgcc tatcaggctt gaatatggcc cggcggaaga ttatgagata aagtccacag
    14941 agaaccgatt ctatttcttt aatccctttt ccataaagat cttcaggaag gttgtaaata
    15001 atattctggc atcggtagcg gagaatccgc gctccgtcga ccttataata tattatccca
    15061 cagctctgta caggcagttt ctaaagaaaa ataccctgtt tgagattata aataaggcca
    15121 aaaccccggg gatgacggac aagaaagaaa agtttcttgt atataggctg agacaggaca
    15181 aacccgacgg aatagtctaa gggggcggct ataaggattt gggtgctgtt tgaaatatta
    15241 taaatatctg cagaggagat attatccatg aagataacgt ttaacggtac aggggcatcg
    15301 gaagggtttc ccgccctatt ttgtgaatgt gagcattgca ccgaggtcag gaccatggac
    15361 cccatcaact ttagaatgcg cagttcctgt ctgatagacg aatccctatt ggtggatttt
    15421 tcttcggata cctatgcaag atgcctttat gggaaactgg atttgaccca ggtaaaagat
    15481 atcatcttta cccattccca ttcagatcat ttctatccag aggatctggg taaaatcgcg
    15541 ccccctttcg ggctgcataa caggaaggag cccctaagga tttatgggaa cagaaatatt
    15601 gggcaggcgt tggaggatat gggcatgacg gggcccgaca aaagtccgta tctgaggttc
    15661 agtccaatgg tggccttcga aacatatcat atagccaatt acaaggttac accccttccg
    15721 gcaaatcacg atccaagcca agaatgcttt atatacgtaa ttcaggggga cgataagacc
    15781 cttctctacg gccatgacag cggttatttt ggcgaagaga cctgggccgg tttaaaaaac
    15841 tttaaatttg acggtgtcat attggactgc accgctgtcg atgaggaatg cccttatccc
    15901 agccatatgg gtcttccgga caatataagg gtcagggaaa ggatgtataa tggcggagca
    15961 gcagatggcc aaacgacctt cattataacc cactttgccc ataccaacgg tccattttat
    16021 gaatacctga atggtttggc acgggaaact ggttttattg ccgcctacga tggacttgag
    16081 ataattcttt aggaagggaa tgaagatccc cgggatttta gactgtcctg cgaaacatat
    16141 aatggagcga aaaggtgtcc ctagcttcca taaggggcga gtctcaatta gttaaaacag
    16201 gtggcgggca tatatatccc tccccattta aacctgcgtg tttcaactgt ttccaatgtt
    16261 ataagtcaga tattttctat ttgccaatcg atgggttctc tacccgttga tgcaagaaaa
    16321 tcgttggctc tggagaaggg tctgctccca aagaaaccgc ggtgggcaga cagagggctt
    16381 ggatggggag attttataat gtaatgctgc ggatcggtta taatactttg cttggactgg
    16441 gcatttcggc cccagaggat aaatacaacg gggtcttccc tctggtttaa tatgcttata
    16501 accctgtcag taaagtgttc ccagcccata ttcttgtggg aatttgcctg ccctgctctt
    16561 acggtcagga cggtatttaa gagcataacc ccctgacggg cccatttttc aaggtagccg
    16621 ttattgggta tataacatcc tagatcgtct tttaattctt tgtagatgtt aagcagtgaa
    16681 ggcggggtct gcactccggg cttgactgaa aagctgagac catgggcctg gtttgggcca
    16741 tgatagggat cttggcccaa tataactgct tttacatcct tatatggcgt atagtgcagg
    16801 gcgttataga tatcgtacat atcgggataa acggtttttg ttctgtattc agctgccaga
    16861 aactgtctca aggctaaata atagtcctta ttaaattcat cttccacatg atcttgccaa
    16921 tcatttttaa gtatttgcat ttttatcacc tacatgtcct atgccggata tccccgcagg
    16981 gtatcgcagg ccatagaaat cttcttattt aatacgttat cgaattcctc tagattatat
    17041 gggtgaatta taccataatt gacaaaatta ttccctatgt ctaaaatgag attataaaaa
    17101 taatcaaacc gggcggcggg taagaatttt ccgccgcgtt gcaacctgat tgtcccaacc
    17161 gctatag
//
LOCUS       DASRNX010000008        12793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_373038,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000008 DASRNX010000000
VERSION     DASRNX010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12793
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373038"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            573..947
                     /locus_tag="VFD89_00260"
     CDS             573..947
                     /locus_tag="VFD89_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56660.1"
                     /translation="MYIPTYLAVLLWVLALFGVVCLMIRLLMGFKWSGRAQKGKYSII
                     ISAKNQEDTIEGLVRGFILKAGIEGQEEALFNVVLVDVGSSDDTSRVMKRLAEEYCYV
                     KFVSCQELPMHLKNIWCMGERE"
     gene            1131..1700
                     /locus_tag="VFD89_00265"
     CDS             1131..1700
                     /locus_tag="VFD89_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZJ56661.1"
                     /translation="MEYVYKDLVERAKAGCKESMGDLIERLRPLIYSVINRYRDRGDM
                     EDLFQDACILIMEAVRDYDEEKGVPFLGFVKSRVYFGIHNKVRRCVFELSLDRPYGDE
                     DGLSPIDTLEDDSIDIEESLINEETSVMLGNAIGELTLKQRGIIRGYYLEGRTLKEMA
                     AARGVHYKAVVQLKARAMEVLRDKIKDSI"
     gene            1812..2036
                     /locus_tag="VFD89_00270"
     CDS             1812..2036
                     /locus_tag="VFD89_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HZJ56662.1"
                     /translation="MALAINPLDSRLQIQFHLGVNEEGKEITRTKSYSRIRSEVSHED
                     LYEVANSLVDLQEHIVVAIRRNSNDEFGEE"
     gene            2072..2284
                     /locus_tag="VFD89_00275"
     CDS             2072..2284
                     /locus_tag="VFD89_00275"
                     /inference="COORDINATES: protein motif:HMM:NF022597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HZJ56663.1"
                     /translation="MSKVLAMNFKTGLGRSMRITLEDPRDDLTSGEVRDAMDLIISKD
                     IFNVEGGVAEIAGASIITTDTQELVF"
     gene            2308..2454
                     /locus_tag="VFD89_00280"
     CDS             2308..2454
                     /locus_tag="VFD89_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HZJ56664.1"
                     /translation="MEQVLGLVANLGFPIVVSVYLLVRIENRMESLTESIHQLAEAVA
                     TFKK"
     gene            2703..3338
                     /locus_tag="VFD89_00285"
     CDS             2703..3338
                     /locus_tag="VFD89_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56665.1"
                     /translation="MNKKANQEVRDWINKGICGLFPIYHEDGGNYTRLIFHHNSPMIV
                     EKRIYTVLVRLAGFYQKDIRLIRQQAREITGQRSLNPLPINPNTILVPFKVRKPIGKD
                     DGAMGYFSSSFIDDIREDEEGVWVFLRDGQGYRVLETLSTARHHMGIAHQIYFHLLRH
                     NAEDGTGYDVCREPAGVYNSVYDQPATKGDMALVFKNIGRLAHILDKLQKR"
     misc_binding    3417..3681
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3891..4904
                     /gene="aroF"
                     /locus_tag="VFD89_00290"
     CDS             3891..4904
                     /gene="aroF"
                     /locus_tag="VFD89_00290"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZJ56666.1"
                     /translation="MIIVMKTGATQKQIQDVERSLQGLGFQTHPIYGAVKTVIGAIGD
                     KRLVKANNINHMEGVENIVPIMSPYKLVGRELIHDNTVINVGDVSVGGKELVVMAGPC
                     AVEDEEQLMDTALKIKEAGAKVLRGGAFKPRTSPYSFQGLEEEGLKLLSEVGRATGLK
                     TVTEVVDTRDVELVSRYADILQVGARSMQNFRLLSECGKSQKPVLLKRGLSATIEEWL
                     MAAEYILSSGSPDVILCERGIRTFETATRNTLDINAIPVVKELSHLPIIVDPSHATGN
                     WRWVDAAAKASIAAGADGLIIEVHPDPSHATSDGQQSLKPEKFTGLMESLRPIAHAVD
                     RDL"
     gene            5128..7395
                     /locus_tag="VFD89_00295"
     CDS             5128..7395
                     /locus_tag="VFD89_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HZJ56667.1"
                     /translation="MDEVVGIIEDVVFRNEDNGFTVMEIRDESQKGTITVVGNLPFAV
                     EGERVKVTGQWAVHPDYGEQLKVENYETASPTSQDGLERYLASGLIKGVGPSTAKRLV
                     EHFGMDTLDIIQFNPDRLTEIPGIGKKKAEAMAASFYEQKEVREVMLFLQTYGISTGF
                     AVKIYNIYGDQTVPIIKENPYRMAQDVVGVGFKTADRIARNMGVLFDSPYRIMAGARY
                     ILSRGANNGHTYLPREELIAKAAQLLGVDLQLVENAIVSLAVDGAVVLEDRSDHTAVY
                     LQPFYVAENNISKRLIELSMIEIASPIDNVDEILYDLQKQEGIVLAENQKEAVVESLK
                     NGVMVMTGGPGTGKTTTVNCIIKLFERLGADVKLAAPTGRAAKRLSETTGQEAKTIHR
                     MLEYGYLGEGEPDLFQKDEDNPLSADVVIIDEMSMVDVLLMNNLLKAIVPGTRLILVG
                     DVDQLPSVGPGNVLRDIINSGLVKVIRLTEIFRQAQESMIVVNAHMINHGEFPKLNLP
                     DKDFFLDRRESPNEILDTLIDLLLRRIPGFGKYHPIRDIQVLSPMRKGVIGVNNLNVQ
                     LQQVLNPPKKNKTEKVFGDMVFREGDKVMQIKNNYNIHWERYDGKNLVEDGDGVFNGD
                     VGYVQAIDEDDREMRVIFEDDRLVSYDFTHLDELELAYAVSVHKSQGSEFPVVVIPLA
                     WGPPMLMTRNLLYTAVTRARKMVVLVGRDRAIDAMVKNNHITNRYSGLEERFRSIFMS
                     LRDLQRFDIFGSAGD"
     gene            7397..7678
                     /locus_tag="VFD89_00300"
     CDS             7397..7678
                     /locus_tag="VFD89_00300"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HZJ56668.1"
                     /translation="MDLWGIWESFLDMVYPTDVRCLVCDNKRQDILAHGVCRMCEASL
                     PFIEPPRCPKCGKMMLADDTLCSDCARADHAFYRGISVFEFGTEVKDLI"
     gene            7706..8143
                     /locus_tag="VFD89_00305"
     CDS             7706..8143
                     /locus_tag="VFD89_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HZJ56669.1"
                     /translation="MSTPMIHWMTGGLESIGWDFDVIVPVPLHPIREKQRGFNQSRLL
                     AKGISENIGVPLMDKSLIRIKNTPQQARLSGHERRKNLMGAFQVKELKNGPGGPDIFE
                     EKTVLLVDDVYTTGSTADQCARVLLGSGAKRVYIITLATGTNL"
     gene            8209..8619
                     /locus_tag="VFD89_00310"
     CDS             8209..8619
                     /locus_tag="VFD89_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZJ56670.1"
                     /translation="MDIRNCRKCNRIFQYRGSRYCPGCMQELDEIFVRVRDYIYDNPQ
                     ATIMEVSQELKVEESIILEFLKEGRLELSSPGLDYVCERCETPITTGRYCNECIQELD
                     RGMKEGLDTPAEKTKGLGRDSQRMHTADFRRRRG"
     gene            8692..8982
                     /gene="flgM"
                     /locus_tag="VFD89_00315"
     CDS             8692..8982
                     /gene="flgM"
                     /locus_tag="VFD89_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HZJ56671.1"
                     /translation="MKINPSHISKAMEIYKAQRPTAPRADRKGQQKQDQVVLSDKAQA
                     FQLALKALQDDDGIDMAKVEAIRKQIAEGEYKINPQEIAEKIVDDLLSKKRV"
     gene            8994..9494
                     /locus_tag="VFD89_00320"
     CDS             8994..9494
                     /locus_tag="VFD89_00320"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HZJ56672.1"
                     /translation="MTDILMGILDSLEKERDFYLELNELSNRKTDIIISKEIKDLERI
                     VDLEQGLIVSIGKLEADRQGLIDELAKIKGINPGSITLSTLIEYSDGDIRDRFQSLQD
                     EFIRIVDRQSHLNEINERLIKTNLEYIDIAINLMAGDGTSGQVYEKEGRAKKGGQNRN
                     LFDSKA"
     gene            9523..11031
                     /gene="flgK"
                     /locus_tag="VFD89_00325"
     CDS             9523..11031
                     /gene="flgK"
                     /locus_tag="VFD89_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HZJ56673.1"
                     /translation="MRSTFYGLEIARKGLFASQKGLDVTGHNIANANTPGYTRQRLVT
                     SSIEPGTGGYGQFTYTAKGQVGSGVTIQELSQIRDKFIDMQYRKENTALGEWTTRTDA
                     LQYIESIFREPSDAGLNAVLADFFTDIQEMAKNPESKEIRALVRQDAIKLTETLHHYH
                     DQMIQLQREQDTAIDITVNEINDIIKNIRDLNEQIFRFEIGGDKANDLRDKRNVLVDE
                     LSSLIKIEYYETSDGKFRVDINGMVLVDHNKYQTLKAVKDVDNPAGEGLDNLYSIKWS
                     GTNIDVAIGGGKLKGYLDIRDGNSPDRMGIPYFVKQLNTFAGALAAEFNRVHEDGWTL
                     PEGDNESRTGIEFFTHERGQAFDPSLITAKNITLSQDIIDSVYNIAASGQEVDMAHPD
                     KQGDNAIALRLAGLRDAKTLPMIGSFENFAKRLIADLAVESSHSQKMLEGQQILTDSL
                     EVNRLSISGVSLDEEMTMMIQYQHAYAAAARTISVMDEALEILINRTGLVGR"
     gene            11044..12003
                     /gene="flgL"
                     /locus_tag="VFD89_00330"
     CDS             11044..12003
                     /gene="flgL"
                     /locus_tag="VFD89_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HZJ56674.1"
                     /translation="MRITNSMMIGQLMNNLNKNLKRMENYQYQLATGSRLQRPSQDPV
                     GITRSLQARTELSKVHQYKKNVDDAGAWLTQTETALTEMNSLIVRAYELAVDGANGDK
                     TPEDRQAIAREVEELMNQLLEASNASYAGRQVFGGYNTTEKPFEVLDDNGQSYLLYNG
                     QRIANLSDPNSQPIIPDGIGSERIEFEIGSGVFMDISMSGTDVLGVGNDNIYAVFHEL
                     YDALNDGTDADRVNESIGKLQFQQQEILSHLAEVGGKTNRLDLIKTRLEMDEMNYTEI
                     KSQVEDVDHEKVIMEFLMAEIVYRSSLSVGARIIQPTLLDFLR"
     gene            12137..12700
                     /locus_tag="VFD89_00335"
     CDS             12137..12700
                     /locus_tag="VFD89_00335"
                     /inference="COORDINATES: protein motif:HMM:NF039791.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6470 family protein"
                     /protein_id="HZJ56675.1"
                     /translation="MDLRITQTYGQIGIDTYRSHMEIEQTPPKANIEQRPPELTIDKK
                     LPKVYIDQTQCFAESGLKPALQLADDFYKEGLQAVFEGIGRMAEEGTRLAAIEKGGNP
                     IADMAYEKLQRNYHLEMVQMPKSRPNIEWDMGHVDIKWQPNPAKIEWDIHTKADITAT
                     RHRVDIYMKEWPDIKIEYVGNNVDSII"
BASE COUNT         3821 a         2650 c         3397 g         2925 t
ORIGIN      
        1 cccctggcac cggactacat agaaaacctg gaaaggatgg cagatacatt ttccaaggtg
       61 cttaaatgaa ggagcatgtt tttgtgatat cggagtattg ataatggcta ttgaacgctc
      121 ctttcaacat cgattcggag aaaactaagg cttatttcat ttgaaaagcc tatcgcttcc
      181 aatccggcct cctgccggtg gaatcggctt aaggcgtcct gccttaaact acggcttttc
      241 tgcattccat aagccaggtt ttcttagcca aatcaatgaa gtcatccgct tttcaatagc
      301 tcactacctt ttttatatag caaaaattct catgataaga aacatccaat aaatcaccct
      361 tttatatgtt ttacgacaaa cattctacat tagtacacag gtgtcgtaaa acattaccct
      421 taattcgtat gttacgaaaa atacattatt ttaacgtaaa atcggataat ttagccgatt
      481 ttataatgaa aggccttttt cgtaacatat ttaggaactc atgcgtacta tgaatcgagt
      541 aggcataaat tgattttccg ggggtgaggc gtatgtatat tcctacatat ttggccgttt
      601 tgctttgggt gttggcgttg ttcggggttg tttgccttat gataaggttg ctcatgggct
      661 ttaagtggag cggcagggct caaaaaggca agtatagcat tataatatcc gcaaaaaacc
      721 aggaggacac cattgagggt ctggtaaggg gctttatttt aaaggcaggt atagaggggc
      781 aggaggaggc cctatttaat gtggtcttgg tagacgtcgg ctcctcggac gatacctccc
      841 gggttatgaa gaggttggcg gaggagtatt gctacgtgaa attcgtttca tgtcaggaat
      901 tgcctatgca tttaaaaaat atctggtgca tgggggagag ggaataacga aaaatcgggc
      961 atgagtaatt gcctgctccg tagaaaacat gatccataac attctgctcc aataaacaag
     1021 aagtcgagga aacaattcct cggcttttcc ttttttggca ccttttttat atgctaagag
     1081 tggttgtaca acacaacaag cgggaccggt cccctttgcg ggggataatt atggaatatg
     1141 tatacaagga cctggtggag agggccaagg ccggatgcaa ggagtccatg ggggatttaa
     1201 tagaaagact ccgtccgctg atctattcgg taataaatcg ttatagggat aggggggata
     1261 tggaggactt gtttcaggat gcgtgtatcc taatcatgga agctgtcagg gattatgatg
     1321 aggaaaaagg ggtgcccttt ttggggtttg ttaagagcag ggtatatttc gggatccata
     1381 acaaggtgcg aaggtgcgtt ttcgagctat ccctggatcg gccctatggg gacgaggacg
     1441 ggcttagccc catagatacg ctagaagatg attctataga tatagaggaa tccctaatta
     1501 atgaagagac atccgttatg ctggggaacg ccataggtga gttaacccta aagcagcggg
     1561 ggataataag gggttactat cttgagggga ggaccctcaa ggagatggcc gcagccaggg
     1621 gcgtacacta taaagcggtt gtgcagctaa aggccagagc catggaagtg cttcgagaca
     1681 aaataaagga ttcaatataa tctttttaaa aatgtatcca ttggcgactg attttgctat
     1741 ctactacatg taagccgaca ataaagtttt ataagcaggc ttaacacccg gcggaaagga
     1801 ggtagtaagc catggcatta gcgattaatc ccttagactc ccgccttcag attcagtttc
     1861 atttgggagt taacgaggag ggtaaggaaa ttacccggac caagagctac agtcgtatta
     1921 ggagcgaggt cagtcatgag gatctctacg aagtagcaaa ttccctggtg gatttgcagg
     1981 agcacatcgt agtagctatc cgtaggaatt ccaatgacga attcggcgaa gagtaggcat
     2041 ttaaaatccg ttgggaaagg gggtgaaacc tatgagtaag gtattagcaa tgaactttaa
     2101 gaccgggctc ggtaggagca tgaggattac cctggaggac cccagagatg atttgacctc
     2161 cggggaggta agggatgcta tggaccttat catctccaag gacatcttta acgtagaagg
     2221 cggcgtggcg gagatagcag gggccagcat aatcactacc gatacccaag agctggtatt
     2281 ttaatagcgg ataggtggtg agttacgatg gagcaggtct taggattggt ggccaacctg
     2341 ggtttcccca tagtagtatc ggtgtatctg ttggtgcgca ttgagaaccg gatggaaagc
     2401 cttacagaat ccattcacca gctggccgag gcagtggcca ccttcaagaa ataaaggata
     2461 aacgtagggg caattcacaa aaatgtgggt tgcccctatg ttaatggtcc taaataatcc
     2521 tattgacacg aacatatgtt cgggttataa tttagaaaac atagttttat gggactgaca
     2581 ttacaaaaaa ctaacccagc tacataagag tatacaagca ttggcaggtc atgtttaggc
     2641 atttcatcgt gggattgact gactaaagaa aatagaaaat agggaagggg gttacagtag
     2701 ttatgaataa aaaagcgaat caagaagttc gagattggat aaacaagggg atatgcggat
     2761 tatttcccat ctatcacgag gacggcggca attataccag gctcatattt catcataact
     2821 cccctatgat agtcgaaaag cggatatata ctgtgctggt gcggcttgcg gggttctacc
     2881 aaaaggatat aaggctgatc aggcaacagg ccagggaaat tacgggccag cgaagcctaa
     2941 atcccctgcc catcaaccca aatacgattt tagtaccctt taaggtcaga aaacccatcg
     3001 gaaaggacga cggggcaatg ggctactttt caagcagctt tattgacgat ataagggagg
     3061 atgaggaagg ggtttgggtc tttttaaggg atggacaagg ctacagggtc ttagagaccc
     3121 tttctaccgc caggcaccat atgggtattg cacaccagat atactttcac ctcctccgcc
     3181 acaatgcaga ggatggcacc ggctacgatg tatgcaggga gcctgccggc gtctacaaca
     3241 gcgtgtacga tcagccggca acaaaaggtg atatggctct ggtatttaaa aacatcggaa
     3301 gactcgccca tattctggac aaactacaaa agcggtaaaa tcaaaaaatg cctattgact
     3361 atctgtagcc aagtagtgta taatttcata caataattaa aaggatttta aatggattaa
     3421 agactatgaa cgggaaaagt aggtttttac caaacccaca gcgaatcggg gagagtgtga
     3481 gcccgatggg taaggaaagg ccgaagttct cccgggagtt gcgggctgaa tgagaaatct
     3541 tgtaggcctt gacggcagcc tttaccgtta tgaaaagggg gatatggcgt ctcttttatt
     3601 ggggattagt attctatttt aaggggacta tttttatagt caaattaggt ggtaacgcgg
     3661 gaataacctc tcgtcctata tattttagga tgaggggttt ttttatttgt ttcagccggt
     3721 tttatggcca ctattgccgg aaggaagtat acggtgatat tttacaaacc ggcgcccgtg
     3781 gttaatcccc atcgtatatt cgcaaaaggg attttcatgt ccggaagacc aacaactgct
     3841 acctaagtca tgaacaaaac gaaaagtcaa aaaagaaagg aaagaaaatc atgatcatag
     3901 taatgaaaac aggagcaacc caaaagcaga tacaggacgt ggaaaggagc cttcagggat
     3961 tgggatttca aacccacccc atatacggcg cggtaaagac ggtaatcggt gccatcggcg
     4021 ataagcggct ggtgaaggcc aataacataa accacatgga gggtgtggag aatatagtac
     4081 ccataatgag cccatataag cttgtcggaa gggaactcat ccacgataac acggtaataa
     4141 atgtggggga tgtgtccgta ggcggaaagg agctggtagt gatggcgggg ccatgtgcag
     4201 tggaggacga ggagcaactt atggatacgg ccctgaagat aaaggaggca ggggccaagg
     4261 tcttaagggg cggggccttc aagcccagga cgtctccata ttccttccaa ggccttgaag
     4321 aggaggggct gaagctttta tcagaggtgg gcagagcgac gggccttaaa accgttacag
     4381 aggtggtgga cacaagggat gtggaattgg tcagccgcta tgcggatatc cttcaggtgg
     4441 gggccaggag catgcaaaac ttcaggcttt taagcgaatg cggaaaatcc caaaagccgg
     4501 tacttttaaa gcgtggactg tcggcaacca tagaggaatg gctcatggca gcggagtaca
     4561 tcctatcatc cgggagcccc gacgttatac tctgcgagcg gggaataagg acctttgaaa
     4621 ccgccacccg aaacaccttg gatataaacg ccatccccgt ggtgaaggaa ttgtcccatc
     4681 tacctataat agttgacccc agccacgcta cggggaactg gaggtgggtg gatgcggcag
     4741 caaaggcttc catagccgcc ggggccgacg gccttataat agaggtgcat cccgacccca
     4801 gccacgctac cagcgacggg cagcaatctt taaaaccaga aaagtttacc gggcttatgg
     4861 agagcctgag acccatagcc catgccgtgg acagggatct gtaggtttgt cgactccccg
     4921 gcccccctat tttaaaaatc taatatacag tcgcacttta agaagctcag cataaaccgg
     4981 ttactgtatt tggacagata atcggaggta tgagaaccgt ccccgcacct ccttccatat
     5041 tgttcaaaag aatcaatgta tagacatgga ttttgtgtta taatttgagt gtagacgaag
     5101 gatcttttaa gcattggggg gccaaggatg gatgaggttg taggtattat agaggatgta
     5161 gtttttagaa acgaggataa cgggttcacc gttatggaga taagggacga atctcaaaag
     5221 ggcaccataa ccgtggtagg aaaccttccc tttgccgtag aaggggagcg ggtaaaggtg
     5281 acgggacaat gggccgtaca cccggattat ggggagcagc taaaggtgga aaactacgag
     5341 actgcatccc ccaccagcca ggatggcctc gaaaggtacc tagcctccgg gcttataaag
     5401 ggtgttgggc cctctactgc caaaaggctg gtggagcact ttggtatgga taccttggac
     5461 atcatccagt ttaaccccga ccgattgacg gagatccccg ggatagggaa aaagaaggcc
     5521 gaggccatgg cggcttcctt ctacgagcag aaggaggtca gagaggtcat gctatttttg
     5581 cagacctacg gcatttctac gggctttgca gtcaagatct acaatatcta tggggatcag
     5641 accgtcccca ttataaagga aaacccctac cgcatggccc aggatgtggt gggcgtggga
     5701 ttcaaaaccg ccgacagaat agcccgaaat atgggagtcc tcttcgattc cccctacagg
     5761 attatggccg gcgccagata tatcctgtcc cggggagcaa acaacgggca tacctatctg
     5821 ccccgagaag agcttatagc caaggcggcc cagcttttgg gggtggatct gcagctggtg
     5881 gagaacgcca tagtgtccct ggccgtagac ggggccgtag tcttggagga caggagcgat
     5941 cataccgcgg tataccttca gcccttttat gtggcggaga acaacatttc aaaaaggctt
     6001 atagagctgt ccatgataga gatagcctct cccatagata atgtggacga gatcctctat
     6061 gaccttcaaa agcaggaagg catagtcctt gcagagaatc aaaaggaagc ggtggtggaa
     6121 tcccttaaaa acggggtcat ggtaatgaca ggggggccgg gcaccggaaa gaccaccacc
     6181 gtcaattgca tcataaagct cttcgaaaga ttaggggcgg atgtaaagct ggccgcaccc
     6241 acgggtaggg cagcaaagag gctttcggag accaccggtc aggaagctaa aaccatccac
     6301 cggatgctgg agtacggtta tttaggggaa ggggagccgg acctttttca aaaggatgag
     6361 gacaaccccc tatcggcgga tgtcgtcatc atcgatgaga tgtccatggt agacgtcctc
     6421 ctcatgaaca atcttttaaa ggccatagtc cccggaacca ggctcatatt agtaggagac
     6481 gtggatcaac tcccctccgt gggtcccgga aacgtgcttc gggatataat aaactccggc
     6541 cttgtaaagg taatacgcct cacagagatc ttccgccaag cccaagagag catgatcgta
     6601 gtcaatgccc atatgataaa ccacggagaa ttcccaaaat taaaccttcc ggacaaggac
     6661 ttctttttag ataggcgtga atcccccaat gaaattttag acaccttaat tgatctttta
     6721 ttaaggcgta tacccgggtt tggaaagtat catcctatca gggatattca ggtgttgtcc
     6781 cccatgagaa aaggcgtaat cggtgtaaac aatctaaacg tccagctgca acaggttcta
     6841 aatcccccaa agaagaacaa gaccgaaaag gtctttggcg atatggtgtt tcgcgagggg
     6901 gataaggtga tgcagataaa gaacaactac aatattcact gggaacgcta cgacggcaaa
     6961 aacttagtgg aggacgggga cggcgtcttt aacggggacg tgggctatgt acaggcaata
     7021 gacgaggatg acagggagat gagggtgata ttcgaagacg atcgcctggt ctcctatgac
     7081 tttactcatc tggatgagtt ggaattagcc tacgcggtgt ccgtccataa gagccagggc
     7141 agcgaatttc ccgtggtggt cattcccttg gcttggggcc cgcccatgct gatgaccaga
     7201 aaccttttgt ataccgccgt taccagggcc aggaagatgg tagttctggt gggcagggat
     7261 agggccatcg atgccatggt aaagaataac cacataacca acaggtattc gggattggag
     7321 gagcgatttc ggagcatctt catgtcctta agggatcttc agagattcga tatcttcggc
     7381 agtgcgggtg actgaaatgg atctatgggg gatttgggaa tcatttttag atatggtcta
     7441 tcctacggat gtaaggtgcc ttgtatgcga caacaagagg caggacattc tagcccatgg
     7501 ggtgtgccgg atgtgtgagg cctccctgcc ttttatagag ccacctaggt gcccaaagtg
     7561 tgggaagatg atgctggccg atgacaccct ttgctcggac tgtgcccggg cggaccatgc
     7621 tttttacagg ggaatatccg tctttgaatt tggtactgaa gtaaaggacc taatctagcg
     7681 atataagtat agaggggaaa aatacttgtc aacccccatg atccattgga tgacaggggg
     7741 attggaatcc ataggctggg attttgacgt gatagtcccg gtacccttac accccataag
     7801 ggaaaagcag cggggcttta atcaatcgcg tcttttagcc aagggcatca gcgaaaatat
     7861 cggggtacct ttaatggaca aaagtctgat taggataaag aatacccccc agcaggcaag
     7921 gctcagcggt catgaaaggc gtaaaaacct tatgggggca tttcaggtaa aggaactaaa
     7981 aaatggaccc ggcggtccgg atatctttga agaaaagacc gtcttgttgg tggacgacgt
     8041 ttacaccacc gggagtaccg ccgatcaatg cgccagggtg ttgttaggga gcggcgcaaa
     8101 aagggtatat attattacct tggccacagg aacaaatctg taacgagtcg ggtaggatct
     8161 ttcatttcat tttatagccc tttgggattt tgaaggagtg atcaatatat ggatataaga
     8221 aactgtcgaa agtgtaatag gatatttcag tataggggca gcaggtattg ccccggatgt
     8281 atgcaggaac tggacgaaat ttttgtaagg gtaagggatt atatctacga taatccccag
     8341 gccaccataa tggaggtttc ccaggaatta aaggtagagg agagcattat tttagagttt
     8401 ttaaaggaag gcaggctgga actgtcatcc cccgggctgg attacgtctg cgaaaggtgc
     8461 gaaacaccca tcaccacggg acgatattgt aacgagtgca tacaggaact ggacaggggg
     8521 atgaaggagg gactggatac tcctgccgaa aaaaccaagg ggctgggccg ggacagtcaa
     8581 aggatgcata ctgctgattt ccgaaggaga aggggataaa taaatttacc ctaaaccttt
     8641 taatgctttg tgtcgatata gtatatgaag acctatgtag gagggaataa agtgaagata
     8701 aatccatccc atatttcaaa ggccatggaa atctataagg cccaaaggcc tacagcccca
     8761 cgtgcggaca gaaaaggaca gcaaaagcag gaccaggtag tcctatccga taaggcccag
     8821 gcttttcagc tggccttaaa ggccctgcaa gatgatgacg gcatagatat ggccaaggta
     8881 gaggccataa gaaagcaaat cgcagagggg gagtacaaga ttaatcccca agagattgcc
     8941 gaaaaaatag tagacgatct gttatccaag aaaagggtat gagaggtatc gctatgacag
     9001 acatactcat gggtatttta gatagcctgg agaaggaaag ggatttttat ttagagctca
     9061 atgagctatc caaccgcaag acggatatta taataagcaa agaaattaaa gaccttgaaa
     9121 gaatcgtaga cctggagcag ggacttatcg taagtatcgg aaaattagag gcggataggc
     9181 aggggctcat tgacgaactt gccaagatta agggtatcaa cccgggatct ataaccctgt
     9241 ccaccctcat agagtacagt gacggggata taagggatag gtttcaaagc ctacaagatg
     9301 agtttataag gatagtcgac aggcaaagcc atttaaacga gataaacgaa aggcttatta
     9361 agaccaactt agaatacata gatatcgcca ttaacctgat ggccggagat gggacgtcgg
     9421 gacaggtata cgaaaaagaa ggacgggcta aaaagggcgg ccagaacaga aacctctttg
     9481 attccaaggc ctaaagatac gaaatttggg agtgaagtat agatgcgatc aactttttac
     9541 ggtttggaga tagcccgcaa gggattgttc gcaagccaaa agggattgga tgtaacggga
     9601 cataatatag ccaatgccaa taccccgggc tacacccggc agcggcttgt gacctcttcc
     9661 atagaacctg ggacaggcgg ctacggacag tttacatata cggcaaaggg ccaggtgggc
     9721 agcggcgtca ccatccagga gctatcccag attagggata agtttataga tatgcagtac
     9781 cggaaggaaa atactgccct gggagaatgg acgaccagga ccgatgccct ccagtatata
     9841 gaatccatat tcagagagcc ttcggatgcg gggctaaatg cggtattggc tgatttcttt
     9901 acggatatac aggagatggc gaaaaatccc gagagcaagg agatacgtgc tctggtcagg
     9961 caggatgcca taaagcttac ggagaccttg catcactacc atgaccagat gatacaactt
    10021 caaagggaac aggatactgc catagatatc accgtaaatg agataaacga tattataaag
    10081 aacatcaggg atcttaatga gcagatcttt aggttcgaga taggcgggga caaggccaac
    10141 gacttgaggg acaagagaaa cgtcttagtc gatgagctgt caagccttat taaaatcgaa
    10201 tactacgaaa cctctgacgg aaagttcagg gtcgatataa acggcatggt cttagtggac
    10261 cataacaagt atcagacttt gaaggccgtt aaggacgttg ataaccctgc cggagaaggg
    10321 ctagataatc tatactccat taaatggtcc ggaaccaaca tagatgtggc catcggcggc
    10381 gggaaactaa aggggtattt agatatcaga gacggcaact cccccgacag gatggggatc
    10441 ccctatttcg tgaagcaatt gaacaccttt gccggtgcct tagctgcaga gtttaatagg
    10501 gtacatgaag atggctggac cttgccggag ggggataacg aatctcgcac gggtattgaa
    10561 ttctttaccc atgaaagagg gcaggccttt gatccgtctt tgataacggc caaaaacatc
    10621 accctcagcc aagacatcat agacagcgtc tacaatatcg ccgcatcagg gcaagaggtg
    10681 gacatggccc atcccgataa gcagggggat aacgccatag ccctaaggct cgcggggctt
    10741 agggatgcca agacccttcc catgatagga agctttgaaa actttgccaa gaggctcata
    10801 gcggacctgg cggttgaatc ctcccacagc caaaagatgc tggaaggcca gcagatctta
    10861 accgacagcc ttgaagtaaa caggctttca atatccggag tatccctgga tgaggagatg
    10921 accatgatga tccaatacca gcatgcctac gccgccgcag ccagaaccat atccgtgatg
    10981 gacgaggcgc tggagatact tataaaccgt acagggcttg ttggaagata gggggaataa
    11041 tccatgcgca taactaattc aatgatgata ggtcagctga tgaacaacct aaataagaac
    11101 cttaagagga tggaaaacta tcaataccag ctggcaacag gtagcaggct acagcgtccg
    11161 tcccaggatc ccgttgggat caccagaagc cttcaggcca ggacggagct ttcaaaagtt
    11221 catcaataca agaaaaatgt agacgatgcg ggtgcctggc tcacccagac ggaaaccgca
    11281 ttaactgaga tgaactccct catagtcaga gcctatgagc tggcggtgga cggcgccaac
    11341 ggggataaga ccccggagga cagacaggcc atcgctaggg aagtagagga gctgatgaat
    11401 cagctcctcg aagcatccaa cgcctcctat gcaggccggc aggtctttgg gggctataac
    11461 accacagaaa aaccctttga ggtattggat gacaacggac aatcctacct actatataac
    11521 ggtcaaagga tcgccaatct gtcggacccg aactctcagc ctatcatacc ggatggtatc
    11581 ggcagcgaaa gaattgaatt tgaaatcggt tcaggagtct ttatggatat ttccatgtcg
    11641 ggaacggatg tcttaggggt aggcaacgac aatatttatg cggtatttca tgaactctac
    11701 gacgccttaa acgacggaac cgatgccgat agggtcaacg aatccattgg aaagcttcag
    11761 tttcagcagc aggaaatcct ctcccacctg gcagaggttg ggggcaagac caatcgcctt
    11821 gacctaatca agacccgatt ggagatggac gagatgaatt atacagaaat aaagtcccag
    11881 gtagaggacg tcgaccatga aaaggttata atggaatttt tgatggccga gatagtgtat
    11941 cgctcttccc tgtccgtagg tgccaggata atccagccta ccttattgga ctttttgagg
    12001 taggaaacaa gcagactggc agctatacag gtatacagac agacagacag aaagaaagat
    12061 agataggtaa atacattgat atgctttccc ataaacccca ttacttactt ctccatatca
    12121 aagataggtg atatatatgg atttgcgtat aacccaaacc tatggacaga tagggataga
    12181 cacctacaga tcccatatgg aaatagagca gactccgccc aaggccaata tagaacaaag
    12241 accgccggaa ctgaccatag ataaaaagct ccctaaggtg tacatagatc aaacccagtg
    12301 ctttgccgaa tcaggcctta aacctgctct gcaactagca gacgatttct ataaggaagg
    12361 ccttcaggcc gtttttgagg gcataggcag gatggccgaa gagggaacaa ggctggcggc
    12421 catagaaaag ggtggaaacc ccattgcgga catggcctat gaaaaactcc aaaggaatta
    12481 tcatttagaa atggttcaga tgccaaaatc aagaccaaac atagaatggg atatgggaca
    12541 tgtggatatt aaatggcaac ccaatcccgc caagatagaa tgggatatcc ataccaaggc
    12601 agatataacc gctacccggc atagggtgga tatctatatg aaggaatggc cggatattaa
    12661 gattgagtat gtgggaaaca atgtcgacag cataatttga taaactcgta tgaagacaat
    12721 gcgttatgcc agttattcca gttaagaata caaattaaga atatacatga taatattgta
    12781 cgtaactata gat
//
LOCUS       DASRNX010000009         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_373136,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000009 DASRNX010000000
VERSION     DASRNX010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373136"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VFD89_00340"
     CDS             <1..1306
                     /locus_tag="VFD89_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HZJ56676.1"
                     /translation="LGGLSPEGEDMVSYFSYMVLEAADWLDSSCNITVRVHDGMDMEF
                     LKKSVYYLFKNKNGWPRFSGDKALSEGFMRKGYPASLARQRVAVGCHWMSLPGLEYTL
                     NDCVKVNCAKVFRLSLDEMMMGEGEKSTENLFELYNKNIRRAVEITAEGIRFHLKYQV
                     YNEPELMLNLISHGPVEKGLDIVDGGAMYYNMCIDGTGIATVADSFAALEQRIEKEEK
                     LTWEMMYEILNNNFHGMEGEYIRRMLESSSRYGGGDTLADRWAVKINEHFTHDVNSMD
                     GDGVKFIPGWFSWSNTIALGKAVEATPNGRRDFEPINHGANPHPGFRKDGALTAMSNA
                     ICKIQPGFGNTAPIQLELDPGMARDDEGIQKMCDYILTIFDLGATLLNINIINADQVL
                     AANEDPDLYPDLVVRVTGFTAYFCLLTPEFRKLVVDRIIKAS"
     gene            1528..2655
                     /locus_tag="VFD89_00345"
     CDS             1528..2655
                     /locus_tag="VFD89_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ56677.1"
                     /translation="MIDNKYAVGIIGCGGMGGGHAIAIASGTGNAIWKSDQSEGTAWE
                     GDLNSDISGKLTLAGIYDIDPARQEWAEDRGFRNYDSYEDMLADENVDIILIATPNDV
                     HKEQAIAAMRAGKHVLCEKPVTPTSAELEEILAVSKETGKVFYPRQNRRWDKDFRIIK
                     KIYDEKLLGDTFNIECRVQGSRGIPGDWRGMKEYGGGMMLDWGVHLLDRMLIMVPEKI
                     KKVFCSLTNITNDEVDDGFKMHITFESGLTSVIEVGTCNFLSLPLWYIAGIDGTAQID
                     NWDCEGKMARLTSWEDKDATPILAGAGLTKTMAPRKGNSVEFLPLPEVDFDNNELYSN
                     LVGTINGTEEQIVTGEQALRVLKLMEAAFESSEKGIVVNFE"
BASE COUNT          787 a          538 c          724 g          710 t
ORIGIN      
        1 attaggggga ttatccccgg aaggggagga catggtctcc tatttctctt atatggtatt
       61 ggaggctgcg gattggctgg actcttcctg taatattacg gtgcgggtcc atgacggcat
      121 ggatatggag ttcctgaaaa aatccgtgta ctaccttttt aagaacaaga acggctggcc
      181 caggttctcc ggggacaagg ccctgagcga aggctttatg agaaagggct atcccgcatc
      241 tctggcacgt cagcgtgtag ccgtgggctg tcactggatg agcctgccgg ggctggagta
      301 tactctaaac gattgtgtta aggttaactg cgccaaggtt ttcaggcttt ccctggacga
      361 gatgatgatg ggggaagggg agaagagtac cgagaaccta tttgaattgt ataataaaaa
      421 cattcgccgg gctgtggaaa taaccgccga agggatacgg tttcatttga aatatcaagt
      481 gtataacgag ccggaactta tgctcaacct aatttcccat gggcctgtcg aaaagggcct
      541 cgacatagta gatgggggag ctatgtacta taatatgtgc atagacggaa ccggcatcgc
      601 tacggtggcg gattctttcg ctgccctgga gcagcgtatc gaaaaggaag aaaagctgac
      661 ttgggaaatg atgtatgaga tattaaataa caatttccat ggtatggagg gcgaatacat
      721 tagaagaatg ctagaatcat ccagccgcta tggcggaggg gatacactgg cggatcgatg
      781 ggctgttaag ataaacgaac attttaccca tgatgtaaac agcatggacg gcgatggggt
      841 taaatttatt ccgggttggt tctcttggtc taacactatc gctttgggta aagccgtaga
      901 ggcaactccc aacggaagaa gggattttga gcccatcaac cacggtgcaa accctcaccc
      961 gggattccga aaggacgggg cgttgacagc tatgagcaat gccatatgca aaatacaacc
     1021 gggttttgga aacactgccc ccatccaact ggaactagat ccgggaatgg ctagggacga
     1081 tgagggtata caaaagatgt gtgattatat actgaccatc ttcgacctag gtgcgaccct
     1141 gcttaacatc aacatcataa acgctgatca ggttttagct gcaaatgagg atccggatct
     1201 atatcccgat ttagtcgtcc gcgttacggg ctttactgca tacttttgcc tcttgacccc
     1261 tgagtttaga aagctcgtgg tagatcgcat tattaaggcg tcatgaaaaa atacgctgtt
     1321 atatatctcc aattagcaaa caaggttgtt taagattata tcaatctgca ggaaaatccc
     1381 agtatcttgc aaacaataca tgcgtatagt ggctttaatg tgaaatttat gatatattgt
     1441 aattaattaa ttgcccaaaa tcatcatttt ctaaagactt ggattctcaa ttgatgtatt
     1501 ggggtataaa ggaaaggtgg aaatattatg attgataaca aatatgccgt cgggattatt
     1561 ggatgcggcg gcatgggtgg tggacacgct attgccattg ccagtgggac cggtaacgca
     1621 atttggaaat cagaccaaag tgaggggact gcttgggaag gggatttaaa ttcagatatt
     1681 tccggaaaat taacacttgc cggtatctat gacatcgacc ccgccagaca ggaatgggcg
     1741 gaagatcgcg ggtttagaaa ctatgacagc tatgaggata tgctagcgga tgagaacgtg
     1801 gatattattc tgatagctac tcccaatgac gttcataagg aacaggccat agccgctatg
     1861 cgtgccggga agcatgtatt gtgtgaaaag cccgtaactc ccaccagcgc agaactggag
     1921 gagatacttg ccgtatctaa ggagacaggt aaggtgtttt atcccaggca aaaccgtaga
     1981 tgggataagg atttccgtat tatcaagaaa atatatgatg agaagctgtt aggtgatacc
     2041 tttaatatag aatgtcgcgt acagggttcc cgtggtatac ccggtgactg gcggggtatg
     2101 aaggaatacg gtggcggtat gatgctggat tggggcgttc atctgcttga tcgtatgctg
     2161 attatggtgc ctgaaaagat caaaaaggtc ttttgctccc tgaccaatat taccaatgac
     2221 gaggtagacg acggctttaa aatgcatata accttcgaga gcggattgac atcggttatc
     2281 gaggtaggaa catgtaattt cctttccttg cccctgtggt atattgccgg cattgacggc
     2341 actgcccaga tcgacaattg ggactgtgaa ggaaaaatgg ctcgtttgac aagctgggaa
     2401 gacaaagatg cgactcctat acttgccgga gccggcctta ccaagaccat ggcaccgcgt
     2461 aaagggaatt cggtagaatt tttaccgcta cccgaagtag attttgacaa caacgagtta
     2521 tatagtaatt tggtgggtac catcaacggt actgaagaac aaatagtcac cggggaacag
     2581 gctctccgag tgctcaagct tatggaggct gcctttgagt cctccgaaaa aggcatcgtg
     2641 gtaaactttg aataaagggt taatgcgtct ttaagttcaa gcttattaaa cggcctaata
     2701 tatagattaa cattaggtca actaaattta ccgtaatgat aattgcaacg gttctatgc
//
LOCUS       DASRNX010000010         3817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_54670,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000010 DASRNX010000000
VERSION     DASRNX010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54670"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFD89_00350"
     CDS             <1..353
                     /locus_tag="VFD89_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HZJ56678.1"
                     /translation="IYKDKIRKHMDRGKTREEAKKLTEKYIAVPGTSEHEIGLAVDFN
                     TLKAEFENAEAFKWLDANAHKYGFILRYPEDKQFITKIKYEPWHFRYVGPAHAQIMKS
                     NNLCLEEYIDYLKD"
     gene            complement(429..908)
                     /locus_tag="VFD89_00355"
     CDS             complement(429..908)
                     /locus_tag="VFD89_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZJ56679.1"
                     /translation="MTSNLSKSARRIQEVITGFGLKLEVVELSDSTRTAQEAADAIGC
                     SVDQIAKSLVFKGKTSKMPVMVVASGSNRVNEKKFKQHIGEALGKADAAFVLEHTGFA
                     IGGVPPIGHTNPVQIFIDEDLMQYSEVWAAAGTPNAVFKISPKSLVDITRGKTINLK"
     gene            complement(1090..2334)
                     /locus_tag="VFD89_00360"
     CDS             complement(1090..2334)
                     /locus_tag="VFD89_00360"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HZJ56680.1"
                     /translation="MLLIDGRLYENADSQWAIDTVSERIIETLTGEPLDIHRVLSASG
                     RLAGDILSGGLNGVLEGFLDDDIMSSYDLVSIASSFKTQELQKRLHLELGHLRKRISK
                     DHNLDIAPLGTLLHIAAGNMDILPAFSVLEGLLSGNINILKLPSMDRGLSIYLLKKMI
                     EYEPCLRNYIYVFDTPSQDFATIQSLMDLVNAIVTWGNDDTIKAIRVSAPINTKIIEF
                     GHKLSFVYLAQSEVPNDRLTALARHIFATKQLLCSSCQVIFLNTHDFEDVKRFGRHFS
                     RIMKEVEHDYSSPMPVQGKVSIELLTRTYERIHSDDVIFRNDKSSVICVNNHELEGSP
                     QLGNVFIKPLPIEKVTKALYKHRSHLQTVGVYPRQRDIENIFIRAGLTNIRDIDDMSS
                     FKLLGSHDGKFPLLEYSRIVEF"
     gene            complement(2339..3457)
                     /locus_tag="VFD89_00365"
     CDS             complement(2339..3457)
                     /locus_tag="VFD89_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56681.1"
                     /translation="MPSSIRKLFRAKDPYDENRNNPYFLKGIKKSTEAFIQVSQSYRD
                     ICGITDFSVHKLVTMGDLYKIPIIPTLFLKRNDISANKKGIITVTSSGTGKKVSVISF
                     TLGEIIGMLRMSITLGRVHGLFSPIPTRYFTLGYEPNKNNRRIISKTAYLSSWYAPGL
                     SRAYALTYKDGEYRLDLDRIIEGLVKSGTGVWPVRIIGFPSYTYFLLEEMKQRDIICR
                     LPKGSRLLLGGGWKQFAGDEIPKGSLYKLVEDVLGVEDSHIHEFFSAAEHPVLYCSCK
                     RHNFHVPVYGKVIIRDVNTLEPLPKGQIGLVNLLTPLGSNLPLMSIMTDDLGILHDGT
                     ECGCGIPTDYLEILGRVGVRDIKTCAVGADDYLKDGEV"
BASE COUNT         1041 a          890 c          796 g         1090 t
ORIGIN      
        1 gtatatataa ggataagata agaaagcata tggacagggg gaaaaccagg gaggaagcca
       61 aaaagctaac ggaaaaatat attgccgtgc ctgggaccag tgaacacgag ataggccttg
      121 cagttgattt taatacctta aaggctgaat ttgaaaacgc tgaggctttt aaatggctgg
      181 atgcaaatgc ccataaatac gggtttatat taagataccc ggaggacaag cagttcataa
      241 caaagataaa atatgaaccc tggcatttta gatatgtcgg ccccgcccat gcccaaataa
      301 tgaagtccaa taatctctgc ctggaagaat atatcgatta cctaaaagat tagccatttt
      361 ctatgcccca tgcctatccc ccatgcctat ccccattatc ggataggcac ggggctttta
      421 tggtcccgct atttgagatt aatggttttt ccgcgggtga tatccaccaa tgatttaggt
      481 gatatcttaa aaaccgcatt tggggttccc gcggcagccc atacctcact atactgcatt
      541 aaatcctcat ctataaatat ttgtacagga ttcgtatgcc cgatgggcgg gacaccgcct
      601 attgcaaacc ccgtatgctc tagaacgaaa gctgcatcgg ccttccccaa ggcttcacca
      661 atatgctgct tgaacttttt ctcgttaacc ctgttagaac cgctggctac gaccataact
      721 ggcattttgg atgttttgcc tttaaaaacc agagattttg cgatctggtc tacggaacaa
      781 ccgatggcat ccgctgcttc ttgggcagta cgggtagaat cggataattc aactacctcc
      841 aatttcaggc cgaatccggt tattacctct tgaattctcc ttgcactttt agataaattt
      901 gatgtcatgc tgataagcct ccttgtataa ttaagttaat gtgttatgct tgtccgttat
      961 ctataatgtt ttacgataat catcctacat tttgtttcag gtatcgtaaa acacataggt
     1021 aatgactatt ggaaaacaag cagaatgcct tttcatatcc agaattcaac aatacggctg
     1081 tattccaatc tagaattcaa caatacggct gtattccaac aaggggaatt tcccatcatg
     1141 ggagcccaac aacttaaagg acgacatatc atcaatatcc cgtatatttg ttaggcccgc
     1201 cctgatgaat atattctcta tatccctttg cctcggataa acccccaccg tctgaaggtg
     1261 tgacctgtgc ttataaaggg cctttgtgac tttttctatg gggaggggtt taataaatac
     1321 gttacccagc tgcggggaac cctccagctc gtgattattg acacaaatga cactgctttt
     1381 atcatttcgg aagatcacat catccgaatg aatcctttca taggtccggg tcaagagttc
     1441 tatggacacc ttgccctgca caggcatcgg gctactataa tcatgttcaa cctctttcat
     1501 aatacgacta aaatgccgcc caaacctttt gacatcttca aaatcatggg tgtttaaaaa
     1561 gataacctgg caggaactgc aaagcagctg cttggtggca aagatatgtc gggcaagggc
     1621 tgtcaatcta tcattcggga cctccgattg ggccagatag acgaagctca gtttgtggcc
     1681 gaattctata atttttgtgt taataggcgc acttaccctt atggctttga tggtatcgtc
     1741 attaccccat gtaacgatgg catttaccaa atccataagg ctttggatag tagcaaaatc
     1801 ctgggaaggg gtatcaaaga catagatata attccttagg cagggttcat attctatcat
     1861 tttcttcaga agatatattg aaagcccccg atccatagag ggcaacttca gtatatttat
     1921 atttcctgat aagagcccct ccaagacgct gaaagccggt aagatatcca tatttcccgc
     1981 ggcgatgtga agcagggttc caaggggggc tatatcgagg ttgtgatcct ttgatatcct
     2041 tttgcgaaga tgccccaatt ccagatgcaa tctcttttgt agttcttggg ttttaaagga
     2101 cgatgcaatg gacactaggt cataggatga catgatatca tcatccagaa aaccctccaa
     2161 tacaccattg agcccccccg ataatatatc cccggccagg cgaccggaag cggataggac
     2221 cctatgaata tccaggggtt cccccgtaag ggtttcaatg attctttcag ataccgtatc
     2281 tatagcccat tggctgtctg cattttcata gagccgaccg tctataagga gcattagtct
     2341 atacctcccc gtcctttaaa taatcatctg cgcccacggc acatgtcttt atatccctga
     2401 ctccgacccg gcctaatatc tccaaataat ccgtcgggat accgcaacca cattctgttc
     2461 cgtcatggag aatccccaaa tcatcggtca taatgctcat taggggtaga tttgatccca
     2521 atggggtcag caggttgacc aagcctattt gtcctttagg caggggttct aaggtattta
     2581 catcccgtat aataaccttg ccgtacaccg ggacgtgaaa gttatgcctc ttgcagctac
     2641 aatacaggac cggatgttct gcggcactaa aaaattcatg tatatggctg tcctctacgc
     2701 ctaggacatc ttccaccagc ttatataggc tgcccttcgg tatctcatcg ccggcaaatt
     2761 gtttccagcc tccgcccagc aaaagcctag accctttggg gagcctgcag ataatatccc
     2821 tttgtttcat ctcctcaagc agaaagtagg tataggaggg gaacccgatg atgcggaccg
     2881 gccaaacccc ggtgccggac ttgactagcc cctcaataat tctgtcgaga tcaagcctgt
     2941 attcaccgtc cttataagtg agggcatagg ctctgctgag gccgggggca taccagctgg
     3001 acaggtaggc ggttttggat attatcctcc tattattttt attaggctca tagcccaagg
     3061 taaaatagcg ggtggggata ggggagaaga ggccatggac ccgccccagg gttatggaca
     3121 tcctaagcat gccgatgatt tcgcccaacg taaagctgat aacgcttacc ttcttaccgg
     3181 ttcccgatga ggttaccgtg attatcccct ttttattcgc agatatatcg tttcgcttga
     3241 gaaagagggt agggattatg ggtattttgt aaaggtcccc catggtcacc agcttatgga
     3301 cggaaaaatc cgttattccg cagatatccc tataagattg ggatacttga ataaaagcct
     3361 ccgttgactt ttttataccc ttgagaaaat aagggttatt gcgattttca tcatacggat
     3421 ctttggccct gaataacttt ctgatactgc ttggcatatt ccccaacccc ttaaaataat
     3481 catacaatct atataccccg atttcaagga tgcgaaatga tttatataat cgctgccaac
     3541 ctcatatttt ctattgaaag tattctgact ctaccggccc atttaccaat tcgtcccata
     3601 ataccattat actttccctc tacttctatg acaagggatt aattcaatct cttatatcgg
     3661 ctcaaaaggt gataaaccgg cataacgtgt taatttaatg gatttgattg attggccacg
     3721 cacactcgtg acttcagtcg tgagttagtg gcctgaatct taattttagt gaattattta
     3781 gtgtgttttc cccccgtgcg tggtataata tatgaag
//
LOCUS       DASRNX010000011        10715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_426357,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000011 DASRNX010000000
VERSION     DASRNX010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10715
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426357"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..806)
                     /locus_tag="VFD89_00370"
     CDS             complement(291..806)
                     /locus_tag="VFD89_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZJ56682.1"
                     /translation="MPVNDIWFMVVFFIFGGILMILELIMPGFGVPGIAGILSLIAGV
                     VVGSSVLTSGQLAVVIFLVFVATIVMAVLLYRSATRNGKLSRLLFLSSSAVKEEGYSS
                     NKSLEDMIGRIGTSTTILRPSGSGDFDGIKVDVIADGQFIPKDTKIKIVKVEGFRILV
                     EQIYKDITDEH"
     gene            complement(1086..2084)
                     /locus_tag="VFD89_00375"
     CDS             complement(1086..2084)
                     /locus_tag="VFD89_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HZJ56683.1"
                     /translation="MKDLIQKLSGVYGPSGNEELVSEAIRGEIESYVDEIRVDVMGNL
                     IAIKKGNGTGKKIMLAAHMDQIGLMITHIDDKGFLRFANLGGISVANSVCRRVLFQNG
                     TVGVIGYETELDSWKDITLAKMYIDIGASNRKEAEGLVKIGDVAVYHPLFSVGNGKYI
                     GGAMDDRAGCALLVKTIRDIGDTPNDLYFVFTVQEELGLRGAKTSAYALEPDLAIAVD
                     VTATGDTPKSKPMAVALGGGPAIKIKDASVLAHPMVKDLMINTAQKHGIPYQLEILEY
                     GGTDSGAIHLSRGGVPSGVLSIPCRYVHSDSETIDAGDMDNGVRLLTRILEENISF"
     gene            complement(2081..3100)
                     /locus_tag="VFD89_00380"
     CDS             complement(2081..3100)
                     /locus_tag="VFD89_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HZJ56684.1"
                     /translation="MSDLLKRLTAAGGVSGDEGRVRAIIIEEITPLVDEVHIDRMGNV
                     TGFKKGTTSNRKVMLCAHMDEVGFIITGIEDNGMLKFATVGSIDSRILPSKRVRIGDN
                     RVPGVMGIKAIHLQEPAERKNAVKARQMYIDIGAGSKDEAERLIKLGDYAVFDSELVY
                     FGDGRVKSKALDDRVGCLTLIESLKGDYPFDLYACFTVQEEVGLRGAKVAAYHIQPDI
                     GLVVEGTTCSDVPGVEPHRHTTRMGNGPAISIADLASYGNKEMVTFLIECAENAGIPF
                     QIKEGVTGGNDAGEIQRSRGGAMTAVISVPCRYIHSPSSVMDLKDLDNTVRLVKEFLK
                     GCESL"
     gene            complement(3085..4137)
                     /locus_tag="VFD89_00385"
     CDS             complement(3085..4137)
                     /locus_tag="VFD89_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HZJ56685.1"
                     /translation="MDAKMFLEELIDTPGVSGYEDEVSKVIKDAFKPYCNEVGRDAFF
                     NVYGKKIGNGGHEGPKVMLAAHLDEIGLMVKNIDESGFISFTNIGGVDQRILLSQEVI
                     IHGKERLYGVIGAKPPHLQEKGETAKAVPMDEMVIDVGFSTEAVKEKITIGDLITFKS
                     PLISLAGTMVSGKSIDDRAGVVMMLETMKELDKLVFSGEIYFVATSQEEVGTRGAIIS
                     TYSLDPDIGIAIDVTHGDTPDAPKDETHAMDKGPVIGMGPNMHPKLTERLLNVARDNE
                     IGHLIEVAPGLTGTDARAMQVSREGVPTVLVELPLRYMHTTVETLDMENVKKAARLTA
                     LFIASLKEGWEECLTY"
     gene            complement(4321..5682)
                     /locus_tag="VFD89_00390"
     CDS             complement(4321..5682)
                     /locus_tag="VFD89_00390"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HZJ56686.1"
                     /translation="MILRELLASTPVFEVIGRRRIDVKGIALHPGDVKKGYLYIFRPE
                     ESTVSYEEAIDQAIQNGAIAIVVGADNKIPKNTITFIRTYHQKRFLSAISRNFYNNPS
                     QNMSLVGITGSHGKTTVGWMIKSILNVAGIPGVMLGIDYCQVGDHSLDSYPGGMNPLN
                     MNEFLYKAVKEQVRWGIIECTYTGIVEDRFSHIWFDGIIYTDLYTYFQNRKADYHYFE
                     MRKTLIDHLKTTQSPVIVNVDDFYAFHLQRDSSVGYGLFNEADITARELVLFPDSSDF
                     VLLTPGGHRRLKLNIPGIHNVYNALAAASWGLAEGLDLDDVVRGIEAFKDMPGLVDRM
                     EFSNSILIKDILDSDLGEIEKAFKNLWEDKKGGIVTVLCMDDSQDVAKYKRLGNIIGR
                     HGGHCIMIHDYFSNSDMAEMESVVEKKMDNIRVHHQTDYYKAIQKAATFLPMGGHILL
                     VRG"
     gene            complement(6098..6652)
                     /locus_tag="VFD89_00395"
     CDS             complement(6098..6652)
                     /locus_tag="VFD89_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZJ56687.1"
                     /translation="MAQEIIISGFGGQGVMSMGQLLTYAGMLEGKHVSWLPSYGPEMR
                     GGTANCSVILADDMIGSPVVTEATALMAMNQPSLEKFERTLISGGLLIVNSSLVNKKA
                     IRDDIDVYYIPANEIADQLGNIRVANMVMLGAYLEKTRGIAPDSVMESLRAVLGSSKE
                     HLIPINRKALEMGAGLVRPANQPI"
     gene            complement(6654..7391)
                     /locus_tag="VFD89_00400"
     CDS             complement(6654..7391)
                     /locus_tag="VFD89_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZJ56688.1"
                     /translation="MAKVFERPKALTDKPFHYCPGCTHGIIHCLIAEVIDELGIREKT
                     VGVAPVGCAVFAYEYFNCDMHEAAHGRAPAVATGIKRVLPDNVVFTYQGDGDLASIGI
                     AEIVHAANRGEKITTIFVNNAIYGMTGGQMAPTSLLGQVTATSPYGRDPETEGHPIKV
                     SEMLATLEGVAYIERVSVHDIRNINRAKRAIKKAFQVQLEGKGFAMIEVLSTCPTNWG
                     MTPVESLKWVKENMIPYYPLGVKKEVD"
     gene            complement(7394..8455)
                     /locus_tag="VFD89_00405"
     CDS             complement(7394..8455)
                     /locus_tag="VFD89_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HZJ56689.1"
                     /translation="MAKVLMKGNEAIAEAAIKAGCRYFFGYPITPQNEIPAYMAKMLP
                     KVGGSFIQAESEVSAINMVYGAAGAGARVMTSSSSPGISLKQEGISYIVGAELPCLIV
                     NIVRGGPGLGGIQPAQSDYFQAVKGGGHGDYHMVVLAPASVQEMADLVMDGFDIADRY
                     RNPVMVLGDGMLGQMMEPVEFQEKDILQPQKPWATTGNRDGEARRIINSLFIDPQGLE
                     EHVLKLYKKYEAMKENETRVECYNCENAEIIIVAYGTTARIAKNVIQLAAQEGINLGL
                     IRPVTLWPFPDKTIGKWAKKSHTKGFLTLEMSMGQMVEDVRLSVNGTKPVYFYGRTGG
                     MVPMPRAVLEEIKKIREGI"
     gene            complement(8701..8907)
                     /locus_tag="VFD89_00410"
     CDS             complement(8701..8907)
                     /locus_tag="VFD89_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZJ56690.1"
                     /translation="MAKVIFDEELCKGCQLCTTVCPKNIVIMDTKRINSKGYHPATVI
                     EMDKCIGCAFCARICPDVVIEVEK"
     gene            complement(8998..9675)
                     /locus_tag="VFD89_00415"
     CDS             complement(8998..9675)
                     /locus_tag="VFD89_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ56691.1"
                     /translation="MSVGSERIAIFTGHFGSGKTELAINYSIAAATEGKKTVVVDLDI
                     VNPFFRSSEKRDVLEEQGIKVISPNFAGTGIDIPSLSAQIYSVFQTRDHKIIFDVGGD
                     DIGATALGSFYPYFKQDDYRVYYVINIRRPLSNNEQDIMDMLESIETNSRLGVTHLIN
                     NSNISYETTIRDLIDGQAVVEGISKVTGKPIAYISGTEEVLMSLPPDLKTKAFPLEIF
                     MTPIWEQ"
     gene            complement(9894..10523)
                     /locus_tag="VFD89_00420"
     CDS             complement(9894..10523)
                     /locus_tag="VFD89_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HZJ56692.1"
                     /translation="MTKAFSTVAMEAETEIIINRSRFIGRVFPVSDEDDALSRLEEIR
                     AEFPDASHHCYAYVIGRGSNIQRFNDDGEPGGTAGMPILQVILQQGIEDVLVVVTRYF
                     GGIKLGAGGLVRAYSKSTAETLQKAGKVDMTPSNKGSISIDYGLWGTLEYFLRQQGIP
                     ILDVDYKEDVTVRIITCMDWDNFSGMITEITDGRAQYEHLGQAYYSWPK"
BASE COUNT         2803 a         2844 c         1973 g         3095 t
ORIGIN      
        1 tttgctaaat gctatttata ccaatatatc ctttagtatt atactagatt tctttttaaa
       61 atcaactaaa aagaatcccg agtatactgc tttaattaat ggatactatt ttttctgttg
      121 taatggctcg ttttctatgt aactttaaac ttccccaatc gctgacccct atggctatat
      181 ccggggtcct catgtgctgg attcctgtgc cggaaattag taagagtata gaataactat
      241 gcaaatgcaa aaaaagatga gtcaagatat gacccatttt ccagatgcgg ttaatgttca
      301 tctgtgatat ctttataaat ctgctccact aagatcctga agccctctac cttaactatc
      361 tttatcttgg tgtcctttgg gatgaactgg ccatcagcta taacgtcgac cttaataccg
      421 tcaaaatccc cggaacctga tggtctcaga atcgtcgtgg aagtacctat cctgcctatc
      481 atgtcctcta agcttttatt gctggaatag ccctcttcct tcacagcgct ggagcttaaa
      541 aacaatagcc ttgataactt tccatttcta gtcgctgatc tatacaacag caccgccatc
      601 actatagtag ctacaaatac caagaaaata actactgcca gttgacccga agttaaaacg
      661 gaagatccca ctacaacccc cgcaatcagg gataggatac ccgcaattcc gggaacgcca
      721 aaccccggca taataagttc cagaatcatt aatataccgc caaatataaa aaatacgacc
      781 atgaaccaaa tatcatttac tggcatttaa tcaccctccc ctttttatat gttacgaaaa
      841 atacgtcaat ttgttgatag gttatacgca acatgcccat ataaaggccc catattaata
      901 tatcatatat ccacggattt ttccactaac ttgccgcaat aggatgcata atctaaattc
      961 gaagaataat atttgcccag tgcaatagaa tgagtaaaaa gggcatttgt ccctggtata
     1021 gcaatggggt ttgcacgatt cgtcgcaaac cccaaacagc taatcgtatt tagtacggtt
     1081 aatcctcaga agcttatatt ctcctccaat attctggtga gcagcctgac cccgttgtcc
     1141 atgtccccgg catctatagt ctcgctgtca ctgtgaacat atctgcaggg gatagaaagt
     1201 accccggatg ggaccccacc tcgggaaaga tgaatagcgc cggaatccgt gcccccatat
     1261 tctaggattt ccaactgata tggaatacca tgtttttgag ctgtatttat cattaaatcc
     1321 ttgaccatag ggtgtgccag gaccgatgca tccttaattt ttatagccgg tcctccgcct
     1381 aacgcaacag ccatgggttt gctcttggga gtatctccag tagctgtgac atcaactgca
     1441 atggccaaat ccggttccag agcataggcc gacgtctttg cgccccttag ccccaactcc
     1501 tcctggacag taaatacgaa atataggtca ttgggagtat caccgatatc ccttatagtc
     1561 ttgaccagca gagcgcaacc ggccctatca tccatagccc cgccgatgta tttgccgttg
     1621 ccgacactaa acaatggatg atataccgcc acatcgccta tctttactaa gccttcggct
     1681 tctttcctat tagaggctcc tatatctatg tacatctttg caagggtaat atccttccag
     1741 ctgtccaatt cggtctcgta acctattacg cccacagtgc cgttttgaaa caacaccctg
     1801 cgacaaacgg aattggctac ggatatgccg ccgagatttg cgaatcttaa aaagccttta
     1861 tcgtcgatgt gggtgatcat cagccctatt tgatccatat gggctgccaa cataattttt
     1921 ttgccggtcc cgttgccctt ttttatggct ataagattcc ccataacatc aacacggatt
     1981 tcatctacat aggattctat ttccccacga atggcctcac tgaccaattc ttcattacct
     2041 gagggtccat aaactcctga taatttttgg attaagtcct tcataaactt tcacatcctt
     2101 ttaagaattc tttaaccaac ctgacagtgt tatctaaatc ctttaaatcc attaccgatg
     2161 aaggtgaatg aatatagcga cacggaacgg atatgacagc agtcatggct cctcctcggg
     2221 atctttggat ttcgcccgca tcgttgccgc cggtaacacc ttccttgatc tgaaatggta
     2281 tgccggcatt ttcggcgcat tctatcagaa aggtgaccat ttctttattt ccgtaagagg
     2341 ccagatcggc aatggaaata gcgggcccgt tacccatcct ggtagtatgt ctatgaggtt
     2401 ccactcccgg tacatccgaa cacgttgttc cctccacgac caatccgata tccggctgta
     2461 tatgatacgc cgctaccttt gcgcccctga gccctacctc ttcttgaacc gtaaagcagg
     2521 cgtacagatc aaagggataa tcccccttga gggattctat gagggtaaga catccaaccc
     2581 tatcatctaa ggccttgctt tttacccttc catctccaaa atagaccagt tcactgtcaa
     2641 atacagcata gtcccccagc ttaatcagtc tttccgcctc gtccttgctt cctgccccga
     2701 tatcaatata catctgcctg gccttaacgg catttttacg ttccgcaggt tcttgaaggt
     2761 gtatagcctt tatgcccatt acccccggaa ccctgttatc cccgatcctt accctcttag
     2821 agggcaggat tctggaatcg attgacccta cagtagcaaa ctttagcata ccattgtctt
     2881 caattccggt aatgataaag cccacttcat ccatatgagc acaaagcatt accttgcgat
     2941 tagaggtggt acctttttta aaacctgtta cattgcccat cctatctata tgtacctcgt
     3001 ctacaagagg cgtaatttcc tcaattatta ttgccctaac ccgcccctcg tcaccggaaa
     3061 ctcccccggc agcagttaat cgttttaata agtcagacat tcttcccatc cctccttcaa
     3121 cgatgctatg aatagagctg tcagtctagc agccttttta acgttttcca tatccaacgt
     3181 ctccacagtg gtgtgcatgt atcttaaggg gagttcaacc aataccgtgg gtacgccctc
     3241 ccgagagacc tgcatagccc tggcatccgt gccggtgaga ccgggcgcaa cctcgatcaa
     3301 atgtcctatt tcgttatccc ttgcgacatt caaaagcctt tcagttaatt ttgggtgcat
     3361 gtttgggccc atgcctataa ccggaccctt atccatggca tgggtttcat cttttggtgc
     3421 gtccggggta tccccatggg taacgtctat ggcgattcct atatccggat caaggctata
     3481 ggtactgatg atggcacccc ttgtccctac ctcttcctgg gaagttgcta caaaatatat
     3541 ttccccggaa aaaaccagct tatctagctc cttcatggtc tcaagcatca tgaccacacc
     3601 ggctctatca tctatggatt taccgctaac catggttcct gcaaggctta tgagcgggct
     3661 tttaaaagtg attaaatccc caattgtaat cttttcctta acggcttccg tggaaaatcc
     3721 aacatcaatc accatctcgt ccatgggaac ggcctttgcc gtttccccct tctcctgtag
     3781 atggggaggc tttgccccta taacaccata tagtctttcc ttaccgtgaa taatgacctc
     3841 ttgggatagt aggatccttt gatctacacc gcctatatta gtgaaactaa taaatccact
     3901 ttcatcaata ttcttaacca tcaggccgat ttcatccaaa tgggcagcaa gcattacctt
     3961 agggccctca tgcccgccat tgccgatctt tttaccatac acattaaaaa aggcatcgcg
     4021 tcccacctca ttacaatagg gcttaaacgc atccttgatt acctttgaaa cctcgtcctc
     4081 ataccccgaa acacctgggg tatcaatcaa ttcttctaga aacattttgg catccatttt
     4141 atcactcctc caacataaag taggccggat catattatta gtggccgtag caacaatacc
     4201 tcagtatcgc tacaattgcc tccgctgttt agcgtagctc ctcgggacca tcggccaaat
     4261 ctgcccttca tcgccgctgc atgcatattc cctgcgtggc catggctatt aaagactatt
     4321 ttacccccta actaatagga tatgaccccc catgggtaaa aatgtagcag ccttttgaat
     4381 agctttataa taatcggttt ggtggtggac tcttatatta tccatttttt tctctactac
     4441 ggattccatc tcggccatat cgctgtttga gaagtagtcg tggatcataa tacaatgtcc
     4501 gccatgtcgg cctatgatat tgcccagcct tttatacttg gccacatcct gggaatcatc
     4561 catacatagt acagtaacta tcccaccttt tttatcttcc cacaggtttt taaatgcctt
     4621 ctctatctcc cctagatccg aatccaaaat atcctttata agaatgctat tggaaaattc
     4681 cattcggtcc actagccccg gcatatcctt aaaggcctct attccccgga caacgtcatc
     4741 cagatccaaa ccttcggcca ggccccaact ggcggcagcc aaggcattat aaacattatg
     4801 tatacccgga atatttagtt taagccttct atgcccccca ggggtcagca gaacaaaatc
     4861 gcttgaatcg gggaagagta ccaattcccg agcggtgata tccgcctcat tgaacagacc
     4921 gtagcctaca ctgctgtcac gttgcaaatg gaaggcatag aaatcgtcga cgttgacgat
     4981 aactgggctc tgggttgtct ttagatgatc tatcagggtc ttcctcatct caaaatagtg
     5041 ataatccgcc tttcggtttt ggaagtacgt atacagatca gtatagataa tgccgtcaaa
     5101 ccagatatgg ctgaatcgat cctccacaat cccggtatac gtacattcga ttatacccca
     5161 gcgaacctgt tcctttacgg ccttgtacag gaattcattc atattgaggg ggttcattcc
     5221 gccgggatac gaatccaatg aatggtcgcc tacctgacaa taatctatac ccaacattac
     5281 cccagggata ccggctacat taagaatgga ttttatcatc cacccgactg tagttttccc
     5341 atgggaacct gtaatcccta ccaggctcat attttgggat ggattgttat aaaaattcct
     5401 ggatatcgcg gagagaaacc gcttttgatg atatgttcta ataaaggtta tggtgttttt
     5461 cggtatttta ttatcagcgc ccactacaat ggctatggca ccgttctgaa tggcttggtc
     5521 tatggcctcc tcgtatgaca ccgtcgattc ttccggccta aaaatgtata gatatccttt
     5581 ttttacatcc cccggatgta gggctatgcc tttaacatca attcttctgc gaccaatcac
     5641 ttcaaagaca ggggtgctag ccagcagttc cctaagtatc atttgattca tcctccccat
     5701 gaattaaaca tcattcagtc catagagccc gattaagagg gtctgggacc tataacccca
     5761 ggcaattggc taatcttcat ataatcaagt atagtatact actttcgcaa atgctttaca
     5821 agataagcta taatcggatt gcagaatgta gattttgaag aatattatcc aattaacttt
     5881 ggttccaaag cctttacact atgtaatttt catataactc ttcaaatatt aaaacagacc
     5941 ctgtgatcgc aaaataaaaa gcccttggga tgttacttat cctagggctg taaataaaag
     6001 cctaacttac gatgattatt ttacggcacg tcccggttaa tggtatttct ggcggacttt
     6061 ggttgaaata cccaactaat cggcctgatc cattacctta gatgggttga ttagcagggc
     6121 gtaccaaccc ggcccccatc tccagagcct ttcgatttat cgggatcaga tgctctttag
     6181 acgaccccaa caccgccctc aaggactcca tcacggaatc cggggctatc ccacgggttt
     6241 tttctaggta tgcgcccagc attaccatat tggccacccg gatatttccc aattgatctg
     6301 caatttcatt ggcaggtata taataaacat caatatcgtc ccttattgct tttttattga
     6361 ctaaggagct atttactata agcaggcccc ccgagataag tgtccgttca aacttttcca
     6421 aggaaggctg attcatagcc ataagagcag tagcttcggt gactacaggc gagccgatca
     6481 tatcgtcggc aaggattaca ctgcagttgg cagttccgcc ccgcatctca ggtccatagg
     6541 aaggcaacca ggaaacatgt tttccctcca gcatcccggc ataggtcaac aattggccca
     6601 tggacataac cccttgtccg ccaaatcctg aaataattat ttcctgagcc atgtcaatct
     6661 acctcctttt tgacaccgag ggggtagtag ggaatcatat tttctttcac ccattttaat
     6721 gattctacgg gggtcatacc ccaattggtg ggacaggtcg ataagacttc gatcatagca
     6781 aagcccttac cttccaattg aacctgaaaa gccttcttta tggctctttt agccctattt
     6841 atattcctta tatcgtgcac tgataccctt tcaatataag caacgccttc caaggtcgcc
     6901 agcatctcgc tgaccttaat gggatgtccc tccgtttccg gatctcgtcc atagggcgaa
     6961 gtcgccgtta cctgccctaa taggctggtg ggcgccatct gaccgccggt catcccatat
     7021 atggcattat ttacgaatat tgttgtgatc ttttcaccgc gattagcagc gtgaactatt
     7081 tccgctatac cgatggatgc caagtcgccg tccccctggt aggtgaacac cacattgtcg
     7141 ggaagaaccc ttttaatacc cgttgctacg gcaggtgccc gtccatgggc cgcttcatgc
     7201 atatcgcaat tgaagtattc ataagcaaat accgcacacc ccaccggagc aaccccaacg
     7261 gttttttccc taattcccag ctcgtctatg acttcggcaa tcaaacaatg aattattcca
     7321 tgggtacagc cgggacaata atgaaaaggc ttgtccgtca acgccttagg tctttcaaac
     7381 acctttgcca tcttcatatt ccctccctta tcttcttaat ttcctccaag acagccctgg
     7441 gcatcggtac cattcccccg gtcctaccgt agaagtatac cggttttgtc ccgttaacac
     7501 tgagcctgac gtcctctacc atctgtccca tgctcatctc caatgtgagg aagccctttg
     7561 tatgactttt ctttgcccat ttccctatgg tcttatcagg aaacggccat aaggtgacgg
     7621 gcctgatcaa ccccaagttt attccttctt gggctgccag ttgaattaca ttcttcgcga
     7681 tccgagctgt ggtgccatat gctacgataa taatttcggc attctcgcaa ttatagcatt
     7741 caaccctggt ctcattttct ttcatcgcct catatttttt atacaatttg agcacgtgtt
     7801 cttcaaggcc ctggggatct atgaataatg aattaatgat ccttctcgcc tcgccatccc
     7861 gatttcccgt agtggcccaa ggtttttggg gctgcaggat atccttctct tgaaattcta
     7921 caggttccat catctgcccc aacatcccat cccccaagac cataaccggg tttcggtatc
     7981 tatcggcaat gtcgaacccg tccatgacaa ggtccgccat ctcctgtacg ctggcaggtg
     8041 ctaggactac catatggtaa tcaccgtgcc cgccgccctt cactgcttgg aagtagtccg
     8101 attgggcagg ttgaattccc ccaagccccg gaccgcccct aacaatattt acaattaaac
     8161 acggcaactc cgccccgact atatatgata tcccttcttg cttcaggctt atgcccggac
     8221 tggacgacga ggtcatgacc ctggcccctg caccggcagc cccatatacc atatttatgg
     8281 cagaaacttc gctctccgcc tgaataaagc ttccacctac tttaggcagc atcttagcca
     8341 tgtaagccgg tatttcattc tgtggtgtta tgggataacc aaaaaaatat cgacagcccg
     8401 ccttaattgc ggcctccgct attgcctcgt tacccttcat caataccttt gccatacatt
     8461 aaaccctcca tttcccaaac tatatatcct gctacaaaca tcctcattag tacctaaacg
     8521 aaatgaaacg ctgtccacaa tccctatgtt acgaataacg catcctttta cataaggtta
     8581 acttacatag agccaaccga tataaacagc attgtaatta aatttctcca taaaacgcat
     8641 caatcatcgt cattcgaaaa gtattataat cccgctttgc ggatgacaat catatgctgt
     8701 ctatttttcc acttcgatta caacatccgg acaaatcctg gcacaaaaag cacaccctat
     8761 gcatttatcc atctctatta ccgttgcggg atgatatccc ttggagttga tacgctttgt
     8821 atccataata acaatatttt tagggcatac agttgtacac aactggcaac ccttgcataa
     8881 ttcctcatca aaaataacct tcgccacctt gaaaccccca atctttgttt cttgaatttc
     8941 gacttgtcaa aaggattatc gccggtcctg tgattgccca ccaaccgcac tgatatttca
     9001 ttgctcccat atgggtgtca tgaagatctc cagtgggaag gccttcgttt taagatcggg
     9061 ggggagtgac atgaggacct cctccgtccc cgagatatag gctatcggtt ttccggttac
     9121 cttggatatc ccctctacaa ccgcctgccc gtctatgaga tctcgaatcg ttgtttcata
     9181 ggatatattg gaattgttta taagatgggt tacccccaac ctggaattgg tttctataga
     9241 ttccaacata tccataatgt cctgttcatt attggacagg ggccttcgaa tatttattac
     9301 atagtagacc ctgtaatcat cctgcttaaa atatggataa aagctgccta gggccgtggc
     9361 ccctatatcg tcccccccta catcaaagat aatcttatgg tctcttgttt gaaataccga
     9421 atatatctgt gcggaaaggg atgggatatc gatgcctgtc ccggcgaaat tcggtgatat
     9481 caccttaatg ccctgctctt ccaaaacatc ccttttctcc gaagatcgaa aaaaaggatt
     9541 gactatatcc aaatcaacga ccacggtttt ctttccttcg gttgcggctg cgatactgta
     9601 gtttatggcc aattccgtct taccgctgcc aaaatgcccc gtaaatatgg ctattctttc
     9661 agacccaaca ctcaaaaata tcacctgcct aaatcagtta tgagaacata tatcaacatg
     9721 gtccaattta caataaaaaa ctgccatctc cagggcggcg aaaataactg caggaattct
     9781 tcaccaccga aatggcaata acgctccctt atcgtgcgct tatttttgca atattttaac
     9841 ttgcaggaat gtatcccccg cttaaatagg tccgggccgc gaattaaccc gttctatttg
     9901 ggccaggaat aatacgcctg cccaagatgc tcgtattggg ccctaccatc ggttatctcc
     9961 gtgatcatgc cggagaaatt atcccagtcc atacaggtaa taatccttac ggtcacgtcc
    10021 tctttataat ccacatccaa tatgggtatg ccttgttgcc ttaagaagta ctctaaggtt
    10081 ccccaaagcc cataatctat ggatattgaa cctttattac tgggagtcat atccacctta
    10141 ccggcctttt gcagggtctc ggctgttgat ttagagtacg cccgaaccaa cccccccgct
    10201 cccaatttaa tgccgccaaa gtatcttgta accactacca atacatcctc tatcccctgc
    10261 tgcaaaatca cctgcagtat aggcattccc gccgtacccc cgggctctcc gtcatcgtta
    10321 aacctttgta tattgctccc acgaccaatt acataggcat agcaatgatg gctggcatcg
    10381 ggaaattctg cgcggatctc ttccagacgt gacagcgcat catcttcatc gctgacaggg
    10441 aatacccggc caataaatct tgagcggttt atgatgattt cagtttcagc ctccatcgct
    10501 acggtactaa atgcttttgt catcgataac cacccattcc aaatctttta ttctattata
    10561 gcacttcttg tctagtaaga aaacccatgg tctgtaaatt aacactatcc aatgcatttg
    10621 acgcatccct tttatttgga taataatatg gaaatcacat tgtgcaaagg cttagaaaat
    10681 attattatcc aaatttagat tatgcacacc aatac
//
LOCUS       DASRNX010000012         8939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_533098,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000012 DASRNX010000000
VERSION     DASRNX010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8939
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533098"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VFD89_00425"
     CDS             <1..1121
                     /locus_tag="VFD89_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZJ56693.1"
                     /translation="CTLYDSEGKSYLDFLGGIAVNTLGYNHPALSEAIIGQAKRLLHC
                     SNHFYIKSQAQLAGILAENSCGDRVFLGNSGAEANEGAIKLARKYFYLKHQYRYEIIT
                     TLNSFHGRTMATLAATGQEKYQRPFEPIPTGFVHVPFNDLEAIINAISYKTCAIMIEP
                     IQGEGGIIEADPGYIRALREICDEKEILLIFDEVQTGIGRTGRLFAYEHYGIEPDIIT
                     LAKGLGGGIPIGAVVAKDSVAKAMEPGDHGTTFGGNPLASSAALAVLMEVTSEGFLEK
                     VNDTAEFFMDQLLELKDKFRFIKEVRGKGLMLGMELDLGIPGKEIVLSMLDRGFVINC
                     AGHNTLRFVPPLIIQKEDIVKLMEALEEEFNDIIKSHS"
     gene            1132..4305
                     /gene="carB"
                     /locus_tag="VFD89_00430"
     CDS             1132..4305
                     /gene="carB"
                     /locus_tag="VFD89_00430"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZJ56694.1"
                     /translation="MPKLESINKVLVIGSGPIIIGQAAEFDYSGTQALKALRDEGIQV
                     ILLNSNPATIMTDLDMGHRIYIEPLTVDIVSKILRIERPQGILPTLGGQTGLNLAMEL
                     AGSGILDELNIRLLGTCLSSIKKAEDRELFKRAMEEIGERVPKSAIANNLDEATRFAM
                     EIGFPIVIRPAYTLGGTGGGIAHNLEQLADIASQGLKSSVIRQILLEESVAGWKEIEY
                     EVIRDNEDNCMIVCGMENLDPVGIHTGDSIVVVPIMTLSDGEQEMLEDAALRIIKALD
                     IKGGCNVQFALHPTGGEYVVIEVNPRVSRSSALASKATGYPIAKVTAKISIGFNLDEI
                     NSSQPVMDYVVTKVPRWPFDKFTSASRTLNTQMKATGEVMAIGSNFEESLLKAIDSLD
                     MGLNYHLGMKAIASWEDSKVLESLENPDDERIFVVSEALRRGYSADKIHGITHIDRYF
                     IEKLENITRLGERLEGTLPEDLDSGLLWECKKYGFGNGYIANLMNTSEEAIENLCLEH
                     GIAPSYQPIDNRLNGDRVKSPYLYSCYDCLGESPRPSSRPKVVVLGSGPIRIGQGIEF
                     DYCSVHSVKTLQEMGIEAIIINNNPETVSTDFDTSDVLYFEPLTYECVMDVLDREKPM
                     GVIVQFGGQTSISLAEPLANAGINILGTTAEDIDRAEDRDKFLQTMTRLGIPLPPGST
                     ALSLKDAQSIAREIGYPVLVRPSYVLGGRAMEIVYNDEELAEYIKTALPASNDRPLLI
                     DKYILGKEVEIDAVSDGVDVLIPGIMEHIERAGIHSGDSFAIYPPQNLSGDIKEKILN
                     YSIRIARGMKIKGLFNIQFIVDNGLLYVLEVNPRASRTVPIMSKITGVSMVNLATRIM
                     LGETLKGLGYRTGIYPESSYVTVKGPVFSFSKLISVDTLLGPEMKSTGEVMGTDTTYP
                     KALKKAMLASGLTFPQGGNVLFALAHGDKELGLEIARSLQERGYGIYATNDTWEYFNL
                     NDLAAQRAEKSRVLDLIRGGGIGLIINTPTLGRISSRLGFSLRRRAMEYNIPCLTSLD
                     TAKAMLDILEDTGSMEVFSLDQYVDRS"
     gene            4659..5582
                     /gene="argF"
                     /locus_tag="VFD89_00435"
     CDS             4659..5582
                     /gene="argF"
                     /locus_tag="VFD89_00435"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZJ56695.1"
                     /translation="MRHLVNLQDYSESEIQDIIRLALRLKSEQKKGISHPLLPNKALG
                     MIFSKASTRTRVSFEVGIRQLGGYGLFLNSSDIQLGRGETIEDTAKVLSRYLDGIMIR
                     TFAQEDVEELAKYSSIPIINGLTDMFHPCQILADLMTIHESKGKLKGLRLAYVGDGNN
                     VAHSLLIGCSKMGLDITIACPIGYEPQRDIVDWAIDNGKSSGSLVNITTQPKEAVGDA
                     DIIYTDVWTSMGQEGESNERLKVFTPYQVNQDLLSLAKPNAIFLHCLPAHRGEEVTSE
                     VIDGPQSLVFEEAENRLHVQKAVLVSLMGGL"
     gene            5655..6155
                     /locus_tag="VFD89_00440"
     CDS             5655..6155
                     /locus_tag="VFD89_00440"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZJ56696.1"
                     /translation="MEIIIKNATIDDIPAIHRITMEAFEGYALNLGLPHQVHALKETY
                     ETIKADLDKKVVLVAWAGDKPVGTIRCEALPPGNVGYISRFGVRPDASNMGVGRALLI
                     AIEKEAAERGLTLLTLHTASKMASLVQFYYKMGYYIHSTTTDRGYIRAYFCKELSCDR
                     DLRMSK"
     gene            complement(6231..6962)
                     /locus_tag="VFD89_00445"
     CDS             complement(6231..6962)
                     /locus_tag="VFD89_00445"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HZJ56697.1"
                     /translation="MIMDRYWKEIGSVYDRVKTEQRDNIVKAGKLIADAVDKGACVHI
                     HDSGHIIDSELIYRGGGLILYKRFRYNLMVENPVRKRDRGDIDTSMEGLAAYALKASG
                     ARPGDVFILGSVSGRTSSVVDLAYEAKKWGMKIIVVTSMSYATTVDSPHSSGQKLHEM
                     ADVVIDNCAPAAEAMMEVEGLEPRLCAASGLSAAFILWSVTTVVVEELMKKGKVPGVL
                     KSANFPGGNEYNRNYVEPRYDKEGY"
     gene            complement(6981..7541)
                     /locus_tag="VFD89_00450"
     CDS             complement(6981..7541)
                     /locus_tag="VFD89_00450"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HZJ56698.1"
                     /translation="MALINPIRAPGLNLDVDPATLTSQIERLPEYGRMILDSQPIGCG
                     DVIIIHSVSGRNTVTIDAALRAKEIGATVIALTNMETTDQVKSRHPSGKNLYEIADLV
                     LDNCGCLGDAALDIPGVPERVAPTSTAIGAAMLNAMMAQAVVLMTVAGAVAPVFVSAN
                     LDRGDAHNKAMLAQYKDNIFYMGHKK"
     gene            complement(7529..7753)
                     /locus_tag="VFD89_00455"
     CDS             complement(7529..7753)
                     /locus_tag="VFD89_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56699.1"
                     /translation="MRKEIDMDAIAAYFGNLKDIIDRVISTQAKALHEGARILSNAAL
                     KGHNLFAFGCAAMQAFWHWNSITGPGAWPS"
     gene            complement(7872..8393)
                     /locus_tag="VFD89_00460"
                     /pseudo
     CDS             complement(7872..8393)
                     /locus_tag="VFD89_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8406..8840)
                     /locus_tag="VFD89_00465"
     CDS             complement(8406..8840)
                     /locus_tag="VFD89_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HZJ56700.1"
                     /translation="MGKILALDGGGTKLNMVLFDNDGFHLLGEGRSGGVNITQTTPEN
                     SRANVADCLRQVFKGERNIHIDRLYSVFIGPMDILNEELARFVTIGEVVELGEAKGGI
                     LAGNMEGEGILALSGTGSDVFYVREQDSLNSSVGGWGPILGD"
BASE COUNT         2372 a         2187 c         2118 g         2262 t
ORIGIN      
        1 gctgtaccct ctacgattcg gaaggcaaat cctacttaga ctttcttggg ggcattgccg
       61 taaataccct gggctataac caccctgcct taagtgaggc tatcataggt caggctaaaa
      121 ggctgcttca ttgttccaac catttctata ttaaatctca ggcccaactg gccggaatcc
      181 tggctgagaa cagctgcggg gatagggtct ttttgggaaa tagcggtgcc gaggccaatg
      241 aaggggctat taagctggcg agaaaatact tttatttaaa gcatcaatat agatatgaaa
      301 tcataaccac cctcaattcc ttccatggca ggaccatggc taccttagcc gctaccggtc
      361 aggaaaaata tcaaagaccc ttcgaaccta tacctacagg gtttgtccat gtgcccttta
      421 acgacttaga agccatcata aatgccatct cctacaaaac ctgcgctata atgatcgaac
      481 ccatccaagg ggagggcggg ataatagagg cagatcccgg atacataaga gccctacggg
      541 agatctgcga cgaaaaggag atcctcctga tcttcgatga agtacagaca ggcataggaa
      601 ggaccggaag gctcttcgcc tatgagcact acggcatcga gcctgacatc attaccctgg
      661 ccaagggcct gggcggcggc atccccatag gcgccgttgt tgcaaaggat tccgttgcaa
      721 aggctatgga acccggagat cacggcacca ccttcggcgg aaatcccctg gcctcctctg
      781 cggcactagc cgtgttgatg gaggttacat cggaaggctt tctggagaag gtcaatgaca
      841 cagccgagtt ctttatggat caactgcttg agttaaagga taagtttcgc tttataaagg
      901 aagttcgggg gaaggggcta atgctgggta tggaactgga tctgggaata cccggcaagg
      961 aaatagtcct ttccatgctg gataggggtt tcgtcattaa ctgcgcaggg cataacaccc
     1021 ttaggtttgt accccccctc attatccaaa aggaagacat agtaaaactc atggaggccc
     1081 tggaggaaga gttcaacgac atcataaagt cacatagcta ggagttaaat catgccaaag
     1141 cttgagagta taaacaaagt attggttatc gggtccggac ctatcataat cggccaggcg
     1201 gcagaattcg attattcagg tacccaagct ttaaaagccc ttagggatga agggattcag
     1261 gtaatcctac taaacagcaa tccggctacc ataatgacgg atttggatat gggccatagg
     1321 atctatatag agcccttaac cgtagatatt gtatctaaga tattgcgtat agaaaggccc
     1381 cagggcatcc ttcccaccct aggagggcag acgggcctaa acctggctat ggaactagca
     1441 ggcagcggga ttttagacga gcttaatatc aggcttttgg gcacctgcct ttcctccatc
     1501 aaaaaggcgg aggacaggga actttttaaa agagccatgg aggaaattgg ggaaagggtc
     1561 cccaaaagtg ccattgcaaa taatcttgat gaggcgaccc ggtttgccat ggagataggt
     1621 ttccccatcg taatacggcc cgcatatacc ttaggcggca ccgggggagg gatagcccat
     1681 aatctagaac aacttgcaga catagcctcc cagggattaa agagcagcgt tatccgccag
     1741 atattattgg aggaaagcgt agcgggctgg aaggaaatcg aatacgaagt gatacgggat
     1801 aatgaggata attgtatgat cgtttgcggt atggagaatt tggaccctgt gggcatccat
     1861 acgggcgaca gcatcgtggt tgtacccatt atgacattat cggatgggga acaggaaatg
     1921 ctggaagatg ccgccctaag gattataaag gccttggata taaaaggagg ctgcaacgta
     1981 caattcgccc tgcatccgac cggcggtgaa tatgtggtta tcgaggttaa ccccagggtc
     2041 agccggtcca gcgccttggc ttccaaggcc acgggttacc ccattgccaa ggttaccgcc
     2101 aagatctcca taggctttaa tttagatgag ataaacagca gccaaccggt tatggattat
     2161 gttgtcacca aggttccccg ctggcccttt gacaagttta catcggcttc cagaacccta
     2221 aatacccaga tgaaagccac aggggaggtt atggccatag gcagcaattt tgaggaatcc
     2281 ctgttaaagg ccatcgactc tttggatatg ggtttaaatt atcatctggg catgaaggcg
     2341 attgcctcct gggaggatag taaggtacta gaatccctgg aaaaccctga cgacgagcgc
     2401 atatttgtcg tatccgaagc cctgagaagg ggctacagcg cggataaaat ccatgggata
     2461 acccatatag acagatattt tattgaaaaa cttgagaaca taacccgttt gggagagagg
     2521 ctggagggca ccttgccgga ggaccttgat agcggacttc tttgggagtg caaaaaatac
     2581 gggtttggca acggctatat agcaaacctc atgaacactt ctgaggaggc catagagaac
     2641 ctttgccttg agcatggtat agccccgtcc tatcagccca tagacaaccg actaaatggg
     2701 gatagggtta aatcccccta tctatattcc tgttatgatt gcctggggga atcccctaga
     2761 ccatcgtccc gcccaaaggt agtagtgctg gggtccggcc ctattcggat aggccagggg
     2821 attgaatttg actattgcag cgttcattct gtaaagaccc ttcaggagat gggaatagaa
     2881 gccattataa tcaataacaa tccggagacc gtgagcacag atttcgatac ctctgatgtc
     2941 ctctattttg aacccctaac ctacgaatgc gttatggatg ttttagatag ggaaaaaccc
     3001 atgggggtga tagtccagtt tgggggccag acctctataa gcctggccga acccctggct
     3061 aatgcgggga taaacatctt ggggaccact gcagaggata tagacagggc agaggacagg
     3121 gataaatttt tgcagaccat gacccggctt ggcatccccc ttccccccgg aagtacagcc
     3181 ctatccctaa aggatgccca aagcatcgca agggagatag gatatccggt attggtcaga
     3241 ccttcatatg tgctgggagg ccgggctatg gagatagtct ataatgatga ggagctggcg
     3301 gaatacatca aaaccgccct tcccgcctcc aatgatcgcc ccttactgat tgacaagtat
     3361 atcttaggca aggaagtcga gatagatgcc gtaagcgacg gcgtagatgt ccttataccg
     3421 ggaatcatgg agcatataga gcgggcgggg atccattcgg gggacagctt tgctatatat
     3481 ccgccccaaa atctaagcgg ggacatcaaa gaaaagatcc taaattacag cataaggata
     3541 gctcgaggga tgaagataaa gggactgttt aatatccagt ttatagtgga taatggcctt
     3601 ttatatgtgc ttgaagtaaa tcccagagcc agccgtaccg ttcccatcat gagtaagatt
     3661 accggcgttt cgatggtcaa tctggccacg aggataatgc tgggggaaac cttaaaaggg
     3721 cttggatata gaaccggtat ttatccggaa tcctcctatg ttactgtaaa aggtccggtc
     3781 ttttccttct ccaagctgat ctcggtggat acccttttag gcccggaaat gaaatccacg
     3841 ggcgaggtca tgggcaccga taccacttat cccaaagctc ttaaaaaggc catgctggcc
     3901 tccggtctta ccttccctca aggcggcaat gtcctctttg ccctggccca tggggataag
     3961 gaattgggcc ttgagatcgc aaggagcctt caggaaaggg gttatggcat ctatgcgacc
     4021 aacgatacct gggagtattt caatttaaat gacttggccg cccaaagggc ggaaaaatcc
     4081 cgggtactgg atctgatcag gggcggcgga atcggcctca ttataaatac gcctaccctg
     4141 ggcaggataa gctccaggct tggattttcc ctaaggagaa gggctatgga atacaatatt
     4201 ccatgcctta cctctttaga taccgcaaag gctatgctgg atattttaga ggataccgga
     4261 tctatggaag tattctccct agatcaatat gtagaccgca gttgaattaa acccttgtgc
     4321 tagttcacta tcatactgcc taacgatggt aatgggttat tgataagcgg atgacttcat
     4381 tgattgggct aagaaaactt ggcttatgga atgcagaaaa gccgtagttt aaggcaggac
     4441 gccttaagcc gattccaccg gcaggaggcc ggattggaag cgataggctt ttcaaatgga
     4501 ataagcatta gttttctcca aatcaatgtc ggaaggagcg ttcaatagcc attaccgaca
     4561 tgattaatta tcacaacaag ttagattaac tccggtcgaa tttacaaact atattttata
     4621 gaggtgtaat aactatgaat acggtaaata aaccgcccat ccgccacctt gtcaatctgc
     4681 aggattatag tgagagtgag atacaagata ttataaggct tgccctaagg ctcaagagcg
     4741 aacagaaaaa gggcatctcc catcctcttc taccaaataa ggctctggga atgatcttct
     4801 caaaggcatc tacccggacc cgggtatcct ttgaggtggg aatacgccaa ttagggggct
     4861 atggtctttt cttaaattct tccgatatcc agctgggacg aggggaaacc atagaggata
     4921 cagccaaggt attgtccaga tatcttgatg ggattatgat taggaccttc gcccaagagg
     4981 atgttgagga gctggctaaa tatagttcca tacccataat caacggcctt acggatatgt
     5041 tccacccttg ccaaatcctg gcggacctta tgaccatcca tgaatctaag ggtaaattga
     5101 agggcttaag acttgcttat gtgggggacg gcaacaatgt agcccattcg cttttgatcg
     5161 gctgctctaa gatgggccta gacatcacca tagcctgccc aatagggtat gaaccccaaa
     5221 gggatatagt tgattgggcc atagataacg gtaaatcttc cggctccctg gttaatatta
     5281 ccacacagcc caaggaggcc gtaggggatg cagacattat atataccgat gtctggacca
     5341 gtatggggca agagggtgag tccaatgaac gactgaaggt ctttactccc tatcaagtca
     5401 accaagactt gctctccttg gcaaagccga atgccatctt tcttcactgt ctgcctgcac
     5461 atcgggggga ggaagtaact tcggaggtaa ttgacggacc acaatcccta gtctttgagg
     5521 aggccgagaa tcggcttcat gtccaaaaag ctgttttggt atcgctgatg ggaggtctgt
     5581 aggaatagaa ttctatttta atgaaagaga tatggatctt tacggaattt acctgaaatt
     5641 cgaggtgaat atatatggaa ataattatta aaaatgctac tattgacgat atcccggcta
     5701 ttcataggat taccatggaa gcctttgaag ggtatgccct caatttgggc cttccccatc
     5761 aggtccatgc cttaaaggag acctatgaga ccattaaggc agatcttgat aaaaaggttg
     5821 tcctagtcgc ctgggccggg gataaacctg tgggcaccat tcgctgtgag gcccttccac
     5881 ccggcaatgt aggctatatc agtaggttcg gagtcaggcc cgatgcctct aatatggggg
     5941 ttggcagggc attattgata gccattgaaa aagaggcggc agagcgtggg ttaactctcc
     6001 ttacccttca taccgcctct aagatggcat ctttggttca attttattac aagatgggct
     6061 actatatcca ttccacaacc acggatcgcg gatatatcag ggcttatttc tgcaaagaat
     6121 tgtcctgcga tcgggattta agaatgtcaa aataattccc ttggccaatc gtatcctgct
     6181 atgtattcta aggcccatat aatttattgc ccagaaaaca acatattcta tcagtatcct
     6241 tccttgtcat atctgggttc cacatagttt ctgttatact cgttacctcc cgggaagttg
     6301 gcgctcttta atactcccgg gacctttcct tttttcatta gttcctctac cactaccgta
     6361 gtcacgctcc ataggatgaa agctgcggat aaaccggatg cagcgcataa tctgggctct
     6421 agaccctcta cctccatcat ggcctcagcc gcgggagcac aattatctat taccacatca
     6481 gccatctcgt ggagcttttg gccgctggag tgaggactgt ccacggttgt agcgtaggac
     6541 atggatgtca ccacaattat cttcattccc cattttttag cctcataggc caggtctacg
     6601 acgctggagg tacggccgga gaccgagccc aagataaaga cgtccccagg ccttgccccg
     6661 gaagccttaa gagcgtaggc cgcaagtcct tccatagagg tatctatatc cccccggtct
     6721 cgttttctta ccgggttttc caccataagg ttgtatctaa acctcttata taatatcaga
     6781 ccgcccccac gatagataag ttccgagtct ataatatgcc ctgaatcatg gatatgtaca
     6841 caggccccct tatcaaccgc atcggcaatt aatttgcccg cttttactat attgtctcgc
     6901 tgttccgtct taaccctatc gtatacggat ccaatttcct tccaatacct atccattatc
     6961 ataatgccat cctcctgtca ttatttttta tgtcccatat agaaaatatt gtccttatat
     7021 tgtgccagca ttgccttgtt atgggcatcc cccctatcta agttagcgga cacaaataca
     7081 ggggcgacgg caccggccac ggtcatcaag actaccgcct gagccatcat ggcgtttagc
     7141 atagcggcgc cgatggctgt ggatgtaggt gctacccttt cgggaacccc cggtatatca
     7201 agagcagcat ccccaagaca tccgcaatta tccaggacca agtccgcaat ctcgtatagg
     7261 ttttttccgc tgggatgccg tgattttacc tgatcggttg tttccatatt ggtcagggca
     7321 atcactgtag cgcctatctc cttagcacgc agcgccgcat ctatggtaac ggtatttcga
     7381 ccggataccg aatgaatgat gatgacatct ccgcatccta tgggttgact gtctaaaatc
     7441 atcctgccgt attcgggcaa ccgctctatc tggctggtta gagtagcggg atccacatcc
     7501 aagttaagcc cgggagccct tatggggttt atgagggcca tgcccccggt cctgtaatag
     7561 agttccaatg ccagaaggct tgcatggctg cgcacccaaa ggcaaagaga ttatggcctt
     7621 taagggccgc attgctgaga atccgagccc cctcatgcaa agcctttgcc tgggtgctta
     7681 taaccctatc gataatgtcc tttaaattcc caaaatatgc cgcaatagcg tccatatcta
     7741 tctcctttcg cacataatat tctaactcct aacgccaatc cattatcttg gtgttcagcg
     7801 ttgattacat cacctggttt tgtataaaaa tcatgtgttt ttaaaacttc ctaactgtca
     7861 ctgcccttat cctaccagtc gattacataa cttgggaatc cctccatgat catttgtttt
     7921 ccttcttcta tggatattcc ctcaagcaac atttcattaa caagacccgc taagacgtgc
     7981 tcaaaccaat gcctctgcac ccgaatcccg gggtatatcg cctctaacct ccggctgaat
     8041 gtctcgaaca tcataggatg ggtcttccac gcgccgccgc agcataccac cttccaatac
     8101 tcccttggaa tcccttcacg gcgaatcagg caatccgtct gcatagccat aaactccccg
     8161 gcctctgcca ggatttcaag ggctacttca tctcccccac gggccgcctc gcccactacc
     8221 ggggttatgg aggccactac cctaaagggc gccactgccc catggatgat cctgaccatc
     8281 tgcctgtcat gggttaaatt ccaatcccgc tggagcaagg gataaatttt ggtaggtttt
     8341 ccccacccgt cgatgtaggt aattaccgcc tttaaggctt cctggcctat ccatgcaccg
     8401 cttccctagt cccctaggat cggtccccag ccacctacag acgaatttag tgaatcctgt
     8461 tctcttacat agaacacgtc ggatcctgta ccggaaaggg ccagaatgcc ttccccttcc
     8521 atattacccg ccaatatccc tcccttggct tcaccaagtt ccacaacctc gcctatggta
     8581 acaaaccttg ccagctcttc gtttaaaata tccataggcc ctataaaaac agaatatagg
     8641 cggtctatgt gtatgtttct ctccccttta aacacctgtc taaggcaatc tgcgacgttt
     8701 gcacggctat tttcgggggt tgtctgggta atgttaaccc cgccgcttcg gccctcaccc
     8761 agcagatgaa atccgtcgtt atcgaacagg accatattga gtttggtgcc cccgccgtct
     8821 aaagctaaaa tcttgcccat gatacaaccc tgcctttcat accccggggc gatttaatcc
     8881 cataatgggg tttagtattt gtaattgtaa ttttagtaca tttaatgcat acgaaaatc
//
LOCUS       DASRNX010000013         6364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_215203,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000013 DASRNX010000000
VERSION     DASRNX010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215203"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            512..1978
                     /gene="mazG"
                     /locus_tag="VFD89_00470"
     CDS             512..1978
                     /gene="mazG"
                     /locus_tag="VFD89_00470"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZJ56701.1"
                     /translation="MKKKITIVGLGPGNENDLTLGALKALKGGNTILRTRQHGIVPFL
                     QDEGISFTALDDIYEDSDTLEGVYSAMIERIIEKAQAAGEAVLGVPGHPMIGDRLVSE
                     LISRLDREEFEIDIVPGVSMGGAVTAITGDRPGPGGLKILMAPDLDINSIDTDTVTVV
                     LDIDSPLGASDVKSCLLRAYPPDLEVYICKNDPDGKPWHRKVALSEVDKQWEYDYSTC
                     LYIPPVELVGLEEFDFRDLVRVMEALRSPEGCPWDREQTHDSLRQYLLEETYEVLEAL
                     DLKDTEKMVEELGDVLLQVVFHGRIGSENGQFDIRDITTGICRKMIERHPHIFGDVKV
                     ENSEQVLDNWEKIKKKEKGLISHTQILKDIPPVLPALMRAYKVQKKAALVGFDWDRIE
                     DAVDKLKEELMELKDAYEGGDRARIDEELGDLLFSAVNVARFLKVEPELSLKATIEKF
                     IRRFEYIEKMAPLPLEQMSLKDMDFLWNEAKTASLKDF"
     gene            2131..2403
                     /locus_tag="VFD89_00475"
     CDS             2131..2403
                     /locus_tag="VFD89_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZJ56702.1"
                     /translation="MNKAELISAVAEKSELTKKDAERAVNSMISVITETLAEGERVQL
                     VGFGTFEVRDRAERKGRNPQTREEIIIPASKAPVFKAGKALKDSVQ"
     gene            2468..2707
                     /locus_tag="VFD89_00480"
     CDS             2468..2707
                     /locus_tag="VFD89_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZJ56703.1"
                     /translation="MRVDKFLKVSRIIKRRTVANQVCSQGRVTVNGRVVKPGAEVKPG
                     DKLEIQFGSGIRSYEILDISQHATKGDAGEMYRIL"
     gene            2950..4011
                     /locus_tag="VFD89_00485"
     CDS             2950..4011
                     /locus_tag="VFD89_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HZJ56704.1"
                     /translation="MRNPKMSYIKVLLCILVIAAMTLGCTALRRPSPKDTPGKEPSKP
                     QMPFPGDTQTKKAVEDQAQKPKIPKILGAGEGQEPNLKVFIKDENRTQEMKMEEYIES
                     VVAGEIKNDWPPEAIKAQAIIARTFALDFIDEKGHSKFGNAHVSTDVEEAQAWNPQAV
                     NDRIKKAVSDTRGQVMVHEGKFAKGWFHSNAAGKTATPKEGLNYKKDNPTYIKPVKSP
                     DDSDKIPADENNWEYKAPKAKVIEALGTMGKPIKDFSKVSISEKGQSGRAVTLAFDDT
                     RVSAPDFRVALSSTDMKSTMLDKVEMEGDQVVFKGRGYGHGVGMSQWGAFKMAEEGKK
                     AEEILSHYFEGIKIVDMWK"
     gene            4121..4423
                     /gene="yabP"
                     /locus_tag="VFD89_00490"
     CDS             4121..4423
                     /gene="yabP"
                     /locus_tag="VFD89_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HZJ56705.1"
                     /translation="MIRDLDDKKTTRGRSHSILMENRQKVSITGVQDVDSFDEATVIL
                     ATDFGYITLHGVDLHISKLNLEEGQLVVEGEIIALQYSDADYSRGKGAGLFSRLFR"
     gene            4436..4840
                     /gene="yabQ"
                     /locus_tag="VFD89_00495"
     CDS             4436..4840
                     /gene="yabQ"
                     /locus_tag="VFD89_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HZJ56706.1"
                     /translation="MTMTTTNQAYIFLATVYGGMLLGIVYDVYRAFRMIIKPGRWFVA
                     VLDLSFWILAVLLSFFMLFKVNGGEIRLFTFIGLGMGWGLYSLIIGGMLVKLLVKVYE
                     IITGIILWPFRTILKAFRWLIRKFRKKDSEDS"
     gene            4986..5279
                     /locus_tag="VFD89_00500"
     CDS             4986..5279
                     /locus_tag="VFD89_00500"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HZJ56707.1"
                     /translation="MKKKRYVLKPRLKIFLALLAIGYFGFNLLKQEIKINEQDDRILD
                     LREKIVQAEDTNAELERLIQFTESEEYIEKVARERLGWVKKGEIIFIEKNNNN"
     gene            5298..5651
                     /locus_tag="VFD89_00505"
     CDS             5298..5651
                     /locus_tag="VFD89_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZJ56708.1"
                     /translation="MPVKVGQILEGTVSGITNFGAFVDLPGGGRGMVHISEVADAYVK
                     DIRDYLKQNDTVRVKVLSIEGNGKIGLSIRRAEAESLVPFEDRLAQFLKDSEERLSDI
                     KNRDNKRGRGSWRGR"
     gene            5860..5936
                     /locus_tag="VFD89_00510"
     tRNA            5860..5936
                     /locus_tag="VFD89_00510"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5894..5896,aa:Met,seq:cat)
     gene            5942..6018
                     /locus_tag="VFD89_00515"
     tRNA            5942..6018
                     /locus_tag="VFD89_00515"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5976..5978,aa:Met,seq:cat)
     gene            6117..6192
                     /locus_tag="VFD89_00520"
     tRNA            6117..6192
                     /locus_tag="VFD89_00520"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6150..6152,aa:Val,seq:tac)
BASE COUNT         1853 a         1273 c         1697 g         1541 t
ORIGIN      
        1 gcccgtcaca aggaccataa ccgaggagga cttgaatgcc ctgcccagag gcagatccat
       61 ggcaaggctt ctgaagaagg cccggctgct tagataggga tgaaggattt gctttgtcag
      121 ctaattattt tatgacttaa cgagtgcttt atctgtaaat atatcggtaa tgagctattg
      181 aacgctcctt tcaacatcga ttcggagaaa actaaggctt atttcatttg aaaagcctat
      241 cgcttccaat ccggcgtcct gccggaggaa gcggcctaag gcgtcctgcc ttatgctacg
      301 gctcttctgc atttcataag ccaagttttc ttagccaaat caatgaagtc atccgcttat
      361 caatagctta tcacccacat aatgaatcgg cataatagct caatggtccc atcggaatat
      421 aaagatatga taggttccgc tgatctttat tttagggtat tatgatgtgt gaaactgaag
      481 gacggaagtt cacggataac tggagggtaa gatgaaaaag aaaataacca tagtgggcct
      541 gggccccgga aatgagaatg acctcacctt gggagccctt aaggccttaa agggcggcaa
      601 tacaatcctt aggacccggc aacatggaat cgtacctttt ttacaagacg agggaatatc
      661 atttaccgcc ctggacgata tatacgagga ctctgacacc ttagagggcg tatattctgc
      721 catgatagag cggatcattg aaaaagccca agctgccggg gaggcagtcc ttggggtacc
      781 cggtcatccc atgataggcg atagattggt ctcagagctc atatcaaggc ttgacaggga
      841 agaatttgag atagatatag tgcccggggt cagcatgggc ggcgcggtca ccgccataac
      901 aggtgacagg ccgggccctg ggggacttaa gatccttatg gcaccggacc ttgatataaa
      961 cagcatagat acggacacag ttacggtggt cttagacatt gacagccccc ttggggcatc
     1021 cgatgtcaag tcctgccttc tcagggcata tcctccggat cttgaggtat acatatgcaa
     1081 gaatgatccc gacggaaagc cctggcacag gaaagttgcc ctcagcgagg tggacaagca
     1141 gtgggaatac gattatagca cctgcctata tatcccacct gtggagttag tgggcctgga
     1201 agaatttgat tttcgggacc ttgtaagggt tatggaggct ttaaggtccc ccgagggatg
     1261 tccctgggac agggaacaga cccatgacag cctgagacaa tacctcctgg aggagaccta
     1321 tgaggtctta gaggccttgg accttaagga taccgagaag atggtggaag aactgggaga
     1381 tgtccttctt caggtggtat ttcacggccg tatcggcagc gagaacgggc aatttgatat
     1441 aagggatatc accaccggga tatgtcgaaa gatgatagag aggcatcccc acatctttgg
     1501 ggatgtaaag gttgagaatt cggagcaggt cctggacaat tgggaaaaga tcaagaagaa
     1561 ggagaaggga cttatcagcc atacccagat actaaaggat ataccccccg tattgccggc
     1621 ccttatgcga gcatacaagg tgcaaaagaa ggctgccctg gtggggtttg attgggaccg
     1681 tatagaggat gcggtggaca agctcaaaga ggaactcatg gagctgaaag acgcctatga
     1741 gggcggggac cgggccagga tagacgaaga actgggggat ctactgttct ccgcggtcaa
     1801 cgtggccaga ttcctaaaag tggagccgga attgtcctta aaggccacca tagagaagtt
     1861 tatacggcgt tttgagtata tagaaaagat ggcgcccctg cccttggagc aaatgtcact
     1921 aaaagacatg gatttccttt ggaatgaagc aaaaacggcg tctttaaagg atttttgatg
     1981 tataacttat gaaataattg gtaaacatat gaatgtcttc acaatttctg tgtataattt
     2041 ataaaaacga taaaaaatat tggaattaag caggaatttg gggaattata gcgaatatgc
     2101 tataaggata actattgtag gaggtttaat atgaataaag cagaattgat ttcagctgta
     2161 gctgaaaaaa gtgagctgac gaaaaaggat gccgaaaggg ccgttaattc catgattagt
     2221 gttattacag agacactagc ggaaggcgaa agggtacaat tagttggttt tggcactttc
     2281 gaagtcagag atagggccga gagaaaaggg aggaatcccc aaaccagaga agagatcatc
     2341 attcccgcct ccaaggctcc tgtattcaag gccggcaagg cgcttaaaga ctctgtacaa
     2401 taatctgttg tgaaggccgg ggttcctaaa gagggtatcc cggtttttgc tttttggggg
     2461 tgaaaatatg agggtggata aatttttaaa ggtatccagg attattaaga gaaggaccgt
     2521 ggccaaccag gtctgcagcc agggcagggt taccgtaaac ggcagggtgg taaagcccgg
     2581 ggccgaggtt aagccggggg ataagctgga aattcagttt gggtccggta taaggagcta
     2641 cgagattttg gatatatccc agcatgccac caagggcgat gcaggtgaga tgtaccgtat
     2701 tctctaggct ccggagcgag cttaaaggtt tttggcctta aaactttagt gattggaata
     2761 ttgttcttca aatttagctt gtgcaccatt atccaaggat gacccatatg tgagtttttg
     2821 tgtaaatcgc ctcattaatg cgaataataa tattgatccc attatccaag cacccgcgga
     2881 ttctatgaaa aggggaatgg tgcttgggat aggatcaatt tgttggcaat aagatgagga
     2941 ggagaaccca tgagaaaccc caaaatgtcg tacattaagg tgcttctttg catattggtg
     3001 atagcagcca tgaccttagg gtgtaccgct ttaaggcggc cgtctcctaa ggataccccg
     3061 ggcaaggaac cgtcaaagcc ccagatgcca ttccctgggg atacccagac taaaaaggca
     3121 gtggaggatc aggcccaaaa gccgaagatc cctaaaatct tgggagccgg agagggccaa
     3181 gagccaaatc tgaaggtctt tattaaagac gaaaaccgga cccaagagat gaagatggaa
     3241 gagtatatag agagcgtcgt agcgggggag ataaaaaacg attggccccc cgaggccatc
     3301 aaggcccagg ccattatagc caggaccttt gctttagact tcatcgacga gaaggggcat
     3361 tccaaatttg gcaatgccca tgtctccacc gacgtggagg aggctcaggc ctggaatccc
     3421 caggcggtaa atgacaggat aaaaaaggcg gtcagcgata cccggggcca ggttatggta
     3481 cacgagggca agttcgccaa gggttggttc cattccaacg ccgccggaaa gaccgcaacc
     3541 cccaaggagg ggctcaacta caaaaaggac aatcccacct acattaaacc ggtaaaatcc
     3601 ccggacgatt cggacaagat acccgccgat gaaaacaact gggaatacaa ggctccaaag
     3661 gccaaggtta tagaggccct ggggaccatg ggcaagccca taaaggattt cagcaaggtg
     3721 agcatttcag agaaagggca atcgggacgg gcagttaccc tggcctttga cgatactcgg
     3781 gtttccgccc cggactttag ggtagccctc tccagcaccg atatgaaatc caccatgctg
     3841 gataaggtgg agatggaggg ggaccaggtg gtatttaagg gcagaggata cggccatgga
     3901 gtgggtatgt cccaatgggg cgccttcaag atggcagagg aggggaaaaa ggcggaggaa
     3961 atcctctccc attatttcga gggaataaag atcgtggata tgtggaagta gcgggacaga
     4021 accctgacta aaaaagtcgg ggttttttta tacccttcta cggacgaaaa tagaatactc
     4081 cccccgggga catcatatac tgtttttaag gggggataac atgatacggg atttggatga
     4141 taagaaaacc acccgaggtc gcagtcatag cattttgatg gagaaccggc agaaggtgag
     4201 cataacaggg gtacaggatg tagacagctt cgacgaagcc acggtaatac tggcaacgga
     4261 ttttgggtat ataaccctcc atggagtaga ccttcatata agcaagctga atcttgagga
     4321 gggccagctg gtagtggaag gggaaattat tgccctccaa tacagcgatg ccgattattc
     4381 caggggcaag ggcgcggggc tgtttagcag gctgtttaga taagggggca tccctatgac
     4441 catgaccact accaaccagg cctatatatt tttagctacg gtgtatgggg gtatgctcct
     4501 tggcatagta tatgatgtgt atagggcctt taggatgatt ataaaacccg gccgatggtt
     4561 tgtagccgtc ctggatctgt ctttttggat tttagctgtt ctcctgtcct tcttcatgct
     4621 gtttaaggtc aacggcgggg agataaggct atttaccttc atcggattgg gtatgggctg
     4681 gggactgtat tccctcatca tagggggaat gctggtgaaa ctgttggtaa aggtttatga
     4741 gataattacc ggcatcatcc tctggccgtt tagaacgatt ttaaaggctt tcagatggct
     4801 gatccgaaag tttcggaaga aggatagcga ggattcctga atgataaagt aaattatacc
     4861 ccaaaaatta aaggaggact tttactttcc ctgtcgaata gtatatacta taaccgtact
     4921 gtatttatat tttatatata gtcatggcta aacgcgtata tatctttttg gtgaggtaga
     4981 ttccaatgaa gaaaaaaaga tatgttttaa aacccaggtt gaagatcttt ttagcgttgc
     5041 tcgcaatagg atactttggg ttcaatctgc tcaaacagga gataaagata aatgagcaag
     5101 acgataggat attggatctt agggaaaaaa tagtccaggc ggaggatacc aatgccgagc
     5161 ttgagcggct gatccaattt accgagagcg aggaatatat cgaaaaggtt gccagagagc
     5221 gtttaggttg ggtgaaaaaa ggtgaaatca ttttcatcga aaagaataat aataattaga
     5281 ggaggaaccc ttgttttatg ccagttaagg taggacaaat tctggaggga acagtttcag
     5341 gaataacgaa ttttggtgcc tttgtagatc tcccgggtgg cgggaggggt atggtgcaca
     5401 tatccgaggt agccgatgcc tatgtcaaag acatccgtga ttatctcaaa caaaatgaca
     5461 cagtgagggt aaaggtatta tccatcgaag gaaacggcaa gataggcctg tccatccgta
     5521 gggcggaagc cgaaagcctt gtaccatttg aggatcggtt ggcccagttt ttaaaggaca
     5581 gcgaggagag actatcagat atcaaaaacc gtgacaacaa gcgcggtagg ggctcctgga
     5641 gaggtagata gcggccttaa taacaaatta aaacgcattc atatatatgg atgcgtttgc
     5701 tataggtaga caattcggaa gctcaattag ctggaattgt ttatctatat attttgctta
     5761 aaaaaccaca taaaaaagtt ttaaaaaaca tcttgacttc tgtatcgatc tgatatacaa
     5821 ttacatttgc cgttaacaag cggctttaat atttaacatc gcggagtaga gcagtcaggt
     5881 agctcgtcgg gctcataacc cggaggtcgg gggttcaaat ccctcctccg caaccatttg
     5941 tggcggcgta gctcagttgg cgagagcatt cggttcatac ccgaagagtc agcggttcaa
     6001 atccgttcgc cgctaccact tttgaagtta gaaattggga gttagaagtc ttgacagcgg
     6061 gaataatccg gctgtttcaa tattagatcc agactcattc acccaatctt caacaggggc
     6121 gcatagctca gctgggagag cacctgcctt acaagcaggg ggtcacaggt tcgagccctg
     6181 ttgtgcccac catacataaa ccgtttgata ccaatgtatc aagcggttta tttttttaag
     6241 ggctaattat tcaccgattt ataattttaa cgtatcgacc ttggggtcat ttttttatcc
     6301 aggattaggt gataatatct ttatctccat tggatccgaa ataaatttgg aaaacatatt
     6361 caac
//
LOCUS       DASRNX010000014         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_215235,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000014 DASRNX010000000
VERSION     DASRNX010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215235"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..739)
                     /locus_tag="VFD89_00525"
     CDS             complement(104..739)
                     /locus_tag="VFD89_00525"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ56709.1"
                     /translation="MIRLKNIDKTYDGLIVLKDFSIDFREGKITALLGPSGCGKTTLL
                     NIVSGLEGYESGTIEGLDDKTFSYIFQEDRLLPWATVGGNLRFVLETGYDEDAIENIL
                     DKYLEMVGLTEYRDYFPSQLSGGMKQRVAIARAFAYPSDILLMDEAFKGLDMELKATL
                     IESFLALWEEDRRGVIIVTHDIDEAMDIAHDIFILGGRPLEIKEKKVGKNK"
     gene            complement(772..1533)
                     /locus_tag="VFD89_00530"
     CDS             complement(772..1533)
                     /locus_tag="VFD89_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZJ56710.1"
                     /translation="MRPSTILKNKRINRGLIVLFWIGVWWIFSLAVNREIYLPSPLST
                     LSAFLRLSIRGDFWYSIAVTFIRVAMGFLVSCLGGIIFGILCGLNRFMYEVFNPLVIA
                     IKSTPVMSVIIIALIWFQSGQVPIFVCFLMCFPVIWTNVVEGVRRVDKGLLQMARVYR
                     VGKRHILRGIYIPSLSPYLMAGMITSLGLGWKVTVAAEVLSNPRYSIGGHLYSAKVYL
                     ESPELFAWTSMVIILSFGFEYLFKRFVGNVTNRGL"
     gene            complement(1505..2488)
                     /locus_tag="VFD89_00535"
     CDS             complement(1505..2488)
                     /locus_tag="VFD89_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HZJ56711.1"
                     /translation="MKKRLIIFVVILAVAIVAISGCRGGDSAPSVTVKVAALNGPTGM
                     GMVKMMEDAKENSSYNYEFSTVGAPDELSGKVISGEFQIAALPTNMASVIYNKTEGQI
                     QLAAINTLGVLYILEDGEDIKEIADLKDKKLNASGKGATPDYILQYLLKENGLDPERD
                     VEIDFSMQHADLAAAVAAGDVSIALLPEPHVTSGLMKNEEIRVALDVTKEWEKVAGDE
                     NPLPMGCIVVQKEFAENNPKIMDDFLAGYEESVKWVNENPEEAGQLIEKHGILPNAKL
                     AEKAIPKSNIVFLDGKGSKGPMDAFLKVLHDLNPASVGGKLPDEAFYYTKE"
BASE COUNT          694 a          719 c          517 g          742 t
ORIGIN      
        1 taccgccggt agggaatttc accctgcccc gaagatagtt aatttatatt cggttttcac
       61 ctatatgata actaatatcc caaaataaat caaggccgga aaattattta tttttcccga
      121 ccttcttttc ctttatctca aggggtctac cccccaagat gaagatatca tgggctatat
      181 ccatagcctc atctatatcg tgggtaacaa ttattacacc ccgcctgtcc tcttcccaaa
      241 gggccagaaa ggattcaatc agagtcgcct ttagctccat atcaaggccc ttaaaggcct
      301 cgtccatgag gagtatatcc gatggatagg caaaggctct ggctatagcc acccgctgct
      361 tcatcccacc gctgagctgg ctcggaaaat agtctctata ctccgttagc cccaccatct
      421 ccaaatactt atccagaata ttttctatcg catcttcatc gtatcccgtc tctaggacaa
      481 accttaaatt gcccccaacg gtggcccagg gaaggagtct gtcctcctga aagatatagc
      541 tgaaggtctt atcatccagg ccctctatgg tccccgattc ataaccttca agtcctgaca
      601 ctatatttaa aagggtggtc ttcccgcagc ccgaaggtcc aagcagggcc gttatcttcc
      661 cctcacgaaa gtctatgcta aaatccttta aaactatcaa tccatcgtaa gtcttatcta
      721 tgttctttag cctgatcatc gtatctgtct ccccataatc attgcggttt cctaaaggcc
      781 gcggtttgtc acattgccca cgaacctttt aaacaggtat tcgaagccaa agcttaggat
      841 gataaccata cttgtccatg caaacaactc cggggattct aagtacacct ttgcactgta
      901 gagatgcccg cctatggaat acctgggatt gctcaacacc tccgccgcca cggttacctt
      961 ccagcccagc cctaaagatg tgatcattcc cgccataaga taaggggata aggacgggat
     1021 atagattccc cttaggatat gccttttgcc gaccctatag accctggcca tctgcagcaa
     1081 tcccttatcc acccgtctta ctccctcgac tacgttggtc catatcacgg gaaaacacat
     1141 tagaaagcat acgaatatgg gcacctgccc agattgaaac catataaggg cgatgattat
     1201 gacggacatc acgggggtgg actttatggc aataaccaag gggttaaaga cttcatacat
     1261 aaatctgttc aggccgcaga ggatgccaaa gattattccc cccaggcatg agactaaaaa
     1321 gcccatggcc acccttataa aggttacggc gatgctgtac caaaaatccc cccttataga
     1381 cagccttaaa aatgcactga gggtacttaa gggggagggg aggtagatct ccctgttaac
     1441 tgcaagggaa aatatccacc acaccccgat ccaaaaaagt actatcagac ccctgtttat
     1501 ccttttattc tttagtatag tagaaggcct catcgggaag cttccccccc accgatgcag
     1561 gattcagatc gtgaaggacc tttaagaagg catccatagg acccttagac cctttcccat
     1621 ctaaaaacac tatattagat tttggtatag ccttttctgc cagcttggca ttgggaagga
     1681 tcccgtgttt ttcgattagt tgccccgcct cttcggggtt ttcattgacc catttaactg
     1741 attcttcgta ccctgccaaa aaatcgtcca ttatcttggg gttattctca gcaaattcct
     1801 tttgcaccac tatgcatccc atgggcaggg gattttcgtc cccggctacc ttctcccatt
     1861 ccttggttac atctagggca accctaatct cttcattttt cataagccct gaggtaacgt
     1921 gaggttccgg cagcagcgca atactgacgt cacctgccgc aactgccgca gccaggtcag
     1981 catgctgcat ggaaaaatct atttccacat ccctctccgg gtccaaaccg ttttccttta
     2041 ggaggtattg gagtatatag tcgggggtgg ctccttttcc gctggcattg agttttttat
     2101 cctttagatc agctatctcc tttatgtcct ccccatcttc cagaatatac aggacgccca
     2161 gggtattgat ggcggccaac tgaatctgtc cctcagtctt attatagata accgatgcca
     2221 tattggtagg cagggccgct atctgaaatt ctccgctgat gaccttaccc gatagttcgt
     2281 ccggggcacc gacggtagaa aattcgtagt tatatgagct gttttcctta gcgtcttcca
     2341 tcatctttac cattcccatg cctgtcggtc cgtttaaagc tgccaccttt accgtgacgg
     2401 aaggagcgct gtctccgccc cgacatcctg atatggccac tatagcgaca gccaaaatta
     2461 caacaaaaat aatgagtctt ttcttcatta caattcctcc atatgtgata tatcttcaac
     2521 acgcatattg tgaaatacat tccgttaact taaattatat caaccgcgta atgttgaata
     2581 ttatcttttt cgcatttatt atatcataag tgcaagcctc gattggcttg cttcacaccg
     2641 attcaataaa cgcattggat aaaacccata gg
//
LOCUS       DASRNX010000015         7908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_692720,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000015 DASRNX010000000
VERSION     DASRNX010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692720"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFD89_00540"
     CDS             <1..231
                     /locus_tag="VFD89_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZJ56712.1"
                     /translation="VYKFIEEEVDRELLNLAMDKLTLREKKIMELRFGLTGGKEQTQK
                     EVADFLGISQSYISRLEKRIIKRLKKEISKMV"
     gene            429..1202
                     /gene="sigG"
                     /locus_tag="VFD89_00545"
     CDS             429..1202
                     /gene="sigG"
                     /locus_tag="VFD89_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HZJ56713.1"
                     /translation="MLVNKVEICGVNTSKLPVLSNERMKELFALMHSGDDSAREEFIQ
                     GNLRLVLSVIKRFNNRGENIDDLFQVGCIGLIKAIDNFDLSHNVRFSTYAVPMIIGEI
                     RRYLRDNNSIRVSRSLRDIAYKALQIRDQLINKNSKEPTVTEIAKELNISREEVVFAL
                     DAIQDPVSLFEPIYNDGGDAIFVMDQVSDEKNLDDAWLEGIALREAMSKLNQREKLIL
                     TLRFFEGRTQMEVAEEIGISQAQVSRLEKTALNHMRKHI"
     gene            1585..1836
                     /locus_tag="VFD89_00550"
     CDS             1585..1836
                     /locus_tag="VFD89_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HZJ56714.1"
                     /translation="MAKSSDFRQKEVINVRNGKKLGVIVDMEFDLLAGRITAIVVPGS
                     NRLMGFFKGDKDYVIPWDKIKKIGDDVILVDVDSNRFES"
     gene            1984..2439
                     /gene="nrdR"
                     /locus_tag="VFD89_00555"
     CDS             1984..2439
                     /gene="nrdR"
                     /locus_tag="VFD89_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZJ56715.1"
                     /translation="MRCPFCGNLDSRVVDSRPTDEGASIRRRRECIECAKRFTTYEKI
                     ETLPIFVIKKDGRRESFNGDKIKVGLVKACEKRPVAIGELDKLVQDIEKAVYNSLELE
                     ITSEKIGQMVMERLQKLDEVAYVRFASVYRQFADVNSFIEELEKIIKDR"
     gene            2585..3286
                     /locus_tag="VFD89_00560"
     CDS             2585..3286
                     /locus_tag="VFD89_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HZJ56716.1"
                     /translation="MSVGLTLLVVAGILFLFGIGHNVLDRMRLSDKAALLFMAGILVG
                     SLVPNIPLGARVSINIGGAIIPLILAIYVFTKAGTAKERTRTVLASILAGIGVYIAGR
                     LLPHEPETMMVDPNYVYGIIAGIIAYLFGRSRRASFIAGIVGVILADIAQGVVNFTNN
                     LPVPVRFGGAGAVDAVVISGFLAVILAEVVGELREKMQGGTKKKQMGFHDGEFINLVG
                     FDAEEEKDDEGAKEK"
     gene            3264..4424
                     /gene="spoIIP"
                     /locus_tag="VFD89_00565"
     CDS             3264..4424
                     /gene="spoIIP"
                     /locus_tag="VFD89_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HZJ56717.1"
                     /translation="MKVQRKNRSVKAIAIILIILATVLPAGKAGADDWYGGEPGFFTL
                     FDEEGGKLTVMASEMFKDDEYISGDNGHYSIYWVDKEGRNAFAKFIGQVELPEFEEVV
                     ETSVETLAPKNGNRSILLYCTHSDESYVPSDGESSIPSKGGIYDVAESLRSSLEKKGV
                     KTVLDRTPHDPHDAGAYRRSRQTAMDLIRKNAPVAAVFDIHRDAIPKEHYDAMVNGEY
                     MSSVRIVIGRSNQNRKANEELAYKIKAIADKTHPGLIKDIYIGRGEYNQELSPRSLLF
                     EFGTHEISKEAAKKATGNIADVVHKAIFGGIFKSKEPALKNTTPGQKQAPKAKQFKVT
                     PISQEKQKGAGRGIMWLFIVAVVGIVVFTLISKSGNEIKSSLENMMGKRKRE"
     gene            4447..4914
                     /locus_tag="VFD89_00570"
     CDS             4447..4914
                     /locus_tag="VFD89_00570"
                     /inference="COORDINATES: protein motif:HMM:NF022827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3189 family protein"
                     /protein_id="HZJ56718.1"
                     /translation="MKVIYYGDATAYAAYGMAAIHLGIYNENEVPHRDDIMKQWKSCL
                     TSSIPRGNLVYMGLDEMLREVYVIGCGSHGRMVKKAYKGFGELYEIHEDILYIDAGPW
                     EGGARILASLSKNYPLIEPIARRIYIRWFKRIYPLWSARVRKGWEMAFKGEMT"
     gene            4911..5384
                     /locus_tag="VFD89_00575"
     CDS             4911..5384
                     /locus_tag="VFD89_00575"
                     /inference="COORDINATES: protein motif:HMM:NF022827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3189 family protein"
                     /protein_id="HZJ56719.1"
                     /translation="MKLFYHCYGGAHTSVTCAAIHLGFLPLDRIPAPNEFLSVAHYDR
                     MENKDLGTPQYFGRDELGIDIYIIGFKNVCNMMIPTIKSYLNANNIPSKDVLFVEALV
                     KLHPITAIGGFSSRKLHMVSLGHPLTVWGIRRSYPIFVELVSKVKENLSQRFPTA"
     gene            5536..6852
                     /gene="der"
                     /locus_tag="VFD89_00580"
     CDS             5536..6852
                     /gene="der"
                     /locus_tag="VFD89_00580"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZJ56720.1"
                     /translation="MAHPIVSIVGRPNVGKSTLFNQLVGRRISIVDYQPGVTRDRVYA
                     DTQWLNTKMTLIDTGGIDTRTQDELMTQMRQQADIAIDTAEVIIFLVDGKEGITPADQ
                     EVASILRRAQKPIVLGVNKIDAPHEEGMLYEFYNLGIGEPISISASHKRGLGDLLDAV
                     AQHLPHGSDETEDDETIKIAVMGKPNVGKSSIVNNLLGEERVIVSDIPGTTRDAIDTP
                     FTLDGQKYVIIDTAGIRRKSRIDEDLERYSVIRALAAIRRCDVALMVIDATQEVTEQD
                     TKIAGLIHEEGKGIILVMNKWDLIEKETNTMNQYRRRLINDLAFMQYAPSIFTSALTG
                     QRMGGIVGMVNEVFSQCTFRISTGVLNDCIMDAVTFSEPPTDKGKRLKIYYGTQVSVK
                     PPTFVLFVNDEKLMHFSYLRYLENHFRKSFGLQGTPIRFIVRQRTN"
     gene            6869..7480
                     /gene="plsY"
                     /locus_tag="VFD89_00585"
     CDS             6869..7480
                     /gene="plsY"
                     /locus_tag="VFD89_00585"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HZJ56721.1"
                     /translation="MRYVLIVVIGYILGNVSSSYLVGKLGSNIDVRQHGSGNLGTTNA
                     YRVLGIKAGALVFIGDVIKGILATIIGLLIVKDSSVGGLIGGIAAVAGHNWPFLLGFK
                     GGKGIATSFGTIIILFPLTAIILLAVVVLIIVLTGYVSLGSITAAVLLPILAIAFRYN
                     PWLIVGAFLLAALAILRHKANISRLLKGTENKITSTGGPRGLK"
BASE COUNT         2352 a         1581 c         2020 g         1955 t
ORIGIN      
        1 gtctataagt ttatagaaga ggaggtggac agggagctct tgaatttggc catggataaa
       61 ctgacgctgc gggagaaaaa aatcatggag ctcagattcg gtctgaccgg gggaaaggag
      121 cagacccaga aggaggtagc cgactttttg gggatctccc aatcatatat ttcaaggctc
      181 gaaaaacgga taattaagag acttaagaag gaaatctcca aaatggtata gactatacca
      241 ccataaacca acattaacat acaaagattg taaaccccat tttagaataa aaaccttggt
      301 ccggggcaat acttcatcta tagagttaac aattaagggt ttcaacttag aatatttttg
      361 atcccatggg tcaacgccat aagtgaaacc cttatcacat actactgcag acggacggag
      421 gttttggtat gcttgtaaat aaagtagaga tctgtggggt caacacttca aaactgccgg
      481 tattatctaa tgagaggatg aaggaattat ttgcccttat gcattccggg gacgacagtg
      541 caagggagga atttatacag ggaaacctta ggctggtttt gagcgtcata aagcgattca
      601 acaaccgagg agaaaacatc gatgacctct ttcaggtcgg atgtatcgga ctcataaaag
      661 caattgataa ttttgatttg tcgcataacg tcagattttc gacctatgcg gttcccatga
      721 taataggaga aattcgcagg tacttaaggg ataacaattc catcagggtc agcagatcct
      781 tgagggatat cgcctataag gcactgcaga taagggatca attgataaac aaaaactcaa
      841 aggaacccac cgttaccgag atagccaaag aactcaatat ctccagggag gaagtagttt
      901 ttgccttaga cgccatccag gatcccgttt cattatttga gcccatatac aacgatggcg
      961 gcgacgctat cttcgttatg gaccaggtca gcgatgagaa gaatctggat gatgcctggc
     1021 ttgagggcat agccttgagg gaggccatga gcaagcttaa ccaacgggaa aagctcatat
     1081 tgaccctcag attctttgag ggcaggaccc agatggaagt cgccgaagaa atcggtatat
     1141 cccaagccca ggtttcacgt ttggaaaaga ccgccctaaa ccatatgcgc aaacacatat
     1201 agataccggt ccggttaatg atcacacagc aattgcgaga gcaattgctt ttattgtata
     1261 acggggcgga tacttttttt cataccctcg tccaaggggg aatataatat attaacctgt
     1321 tgtgcaatat atgttacgaa aaagatcttt caatacaaaa tcagttaagt tatctgattt
     1381 tcgttatagc aatgtatttt tcgtaacata tgaattgagg ataatgtttt acgacacccg
     1441 tatactaatg tagaatgttt gtcgtaaaac atatagttaa acatactggt aatggctatt
     1501 atcaaatggc tattatcaca caataggata atgtactggc ataatgcgct atattaatcc
     1561 aaaataatag gggtgatggg tgtcatggca aagtcttcag actttcggca aaaggaagtt
     1621 ataaacgttc gaaacggtaa gaaactcggt gttatagtcg atatggaatt cgatctctta
     1681 gccggcagaa taacagcgat tgtagttccg ggctctaaca ggctcatggg atttttcaag
     1741 ggggataagg attatgtaat accctgggac aagataaaaa agataggtga tgatgtcata
     1801 ctggtggatg tagattccaa tagattcgaa agttaagacg attttgtgaa ttggttgatt
     1861 tttgtaaatt gattgcaaaa ttgtatggac aagtgtgaca aatatgggta caatgtacta
     1921 aaggttagga gttttgtacc aaaatttgaa ggtaatagac taggaacggg ggtttttgta
     1981 cgtatgaggt gtcctttctg tggaaattta gatagcaggg ttgtggattc ccggcctaca
     2041 gacgaaggtg ccagtatcag gaggcgcagg gagtgtatag aatgcgcgaa aaggtttacc
     2101 acctatgaaa agatcgagac attgccaatc tttgttataa aaaaggacgg cagaagggaa
     2161 tcttttaatg gggacaagat aaaggtagga cttgtgaagg catgtgagaa aaggccagta
     2221 gctatagggg aattggataa gttggtacaa gacatcgaaa aggccgtata taactctctg
     2281 gagctggaaa taaccagcga aaaaataggc cagatggtca tggaaaggct tcaaaagctg
     2341 gacgaagtcg cctatgtacg ctttgcgtct gtctacagac aattcgcaga tgtcaacagc
     2401 ttcatagagg agctggaaaa gatcataaag gacagataac agcggattaa ctcgcacaca
     2461 agagccatat agaggggctc tttttttgtt cgaatacaac tattgcgacc tgggcaatat
     2521 aactgtgatt gagaaaatct caataacata ttgatgactc caattcatag gacgaggtga
     2581 taatatgtct gtaggtctga ctctgttggt cgttgcgggg atcctgtttt tgtttggcat
     2641 aggtcataac gtattggatc gaatgcgcct tagcgataaa gccgcactgc tcttcatggc
     2701 aggaattctt gtaggcagtt tggtgcccaa catcccctta ggggccaggg tttctataaa
     2761 tatcggcggg gctataatcc cccttatact ggctatatac gtatttacaa aggcggggac
     2821 cgccaaggaa agaacccgaa ctgtcctggc atctatcctg gcggggatag gggtttatat
     2881 tgccgggcgg ctccttcccc acgagccgga gaccatgatg gtggatccaa actatgtcta
     2941 cggtatcata gcggggataa tagcttattt gtttggcaga tccaggcgcg cttcatttat
     3001 agcgggcata gtcggggtga ttttggccga tattgcccag ggcgtagtta actttaccaa
     3061 taacttacct gtccctgtac gctttggtgg ggcgggagcg gtggatgcag tagtcatatc
     3121 cggatttttg gcggttattc tggccgaagt agtgggtgag ctacgggaga aaatgcaggg
     3181 aggaaccaag aaaaagcaga tgggctttca tgacggcgaa ttcatcaact tggttggctt
     3241 cgatgcagag gaggaaaagg acgatgaagg tgcaaaggaa aaatagatcc gtcaaggcga
     3301 tagcgataat tctgattata ttggctactg tgttgccggc gggcaaagcg ggggcggatg
     3361 attggtatgg tggggaaccg ggttttttta ccctctttga tgaagagggc gggaagctta
     3421 cggttatggc cagcgagatg ttcaaggacg atgaatatat cagcggtgac aacggacact
     3481 actcgatcta ttgggtggac aaggaaggca gaaacgcatt cgcaaaattc atcggccaag
     3541 tagagctccc cgaattcgaa gaggtagtgg aaacatccgt tgagaccctt gcaccaaaga
     3601 atgggaacag gagcattttg ctgtattgca cccatagcga cgaatcctac gtccccagcg
     3661 acggggagtc cagcattccg tctaaaggag gtatctacga cgtcgcagag agcctcaggt
     3721 catccctcga aaaaaagggt gtaaagacgg tcctggatag gacgccccac gacccccacg
     3781 atgcaggggc atatcgccgg tccaggcaaa ccgccatgga ccttataaga aaaaatgctc
     3841 cggtggccgc agtatttgat atccacaggg atgcgatccc caaggaacat tacgatgcca
     3901 tggtaaatgg ggaatatatg agcagcgtca ggatagttat tggcagaagc aaccagaata
     3961 gaaaggccaa cgaagaatta gcctacaaga taaaggctat agccgacaag acccatcccg
     4021 ggctcatcaa ggatatatat ataggaaggg gagaatacaa tcaggagctg tccccgcgtt
     4081 cccttctctt cgaattcgga acccatgaga taagcaagga ggcggccaaa aaagccaccg
     4141 gtaatatagc ggatgtagta cataaggcaa tattcggcgg catattcaaa tccaaggaac
     4201 ccgctctaaa aaacacaacc ccgggacaaa aacaagcccc aaaagcgaaa caatttaagg
     4261 taacccccat aagccaagaa aagcaaaagg gtgcgggcag gggtattatg tggcttttta
     4321 tagtagccgt tgtaggaatc gtagtcttta ctctgatcag caagagcggg aatgagataa
     4381 aaagcagctt agagaacatg atgggaaaga gaaaaaggga atgatcgtta cgcattaggg
     4441 gttggcatga aggttatcta ttatggcgat gctaccgcct atgcggctta tgggatggca
     4501 gcaatccatt tgggcatcta taatgagaat gaggttcccc atcgggatga tataatgaaa
     4561 caatggaagt cgtgtctcac cagcagcatc ccaaggggaa acctcgtcta catggggctg
     4621 gatgaaatgc tcagggaagt gtatgttatt ggatgtggca gtcacggcag gatggtcaaa
     4681 aaggcatata aggggtttgg cgaactctac gaaatccatg aggatatact ctatatagat
     4741 gccggtccct gggaaggggg agccaggatt ttggcatccc tctccaaaaa ctacccgctg
     4801 atcgaaccca tcgcgagaag aatctatatc cgctggttta aaagaatcta tcccctgtgg
     4861 tccgccaggg tgcgaaaggg gtgggaaatg gctttcaaag gggaaatgac gtgaaactct
     4921 tctatcattg ctacggcggc gcccatacat ccgtcacgtg cgcagccatc catttaggat
     4981 ttttgccttt ggatcgaatc ccggccccca acgaattttt gtccgttgcg cactatgaca
     5041 ggatggaaaa taaggacctg gggacacccc aatattttgg gagggatgag ctgggaattg
     5101 atatctatat tataggattt aaaaacgtat gcaatatgat gatacccaca attaagagtt
     5161 acctaaatgc caataacatc ccatctaagg atgttttatt tgtagaagcc ttggtgaagc
     5221 tccacccaat taccgccata ggaggctttt cttcaagaaa gctccatatg gtcagcttag
     5281 ggcacccctt aacggtatgg ggcataagga ggagctaccc catatttgta gaattggtat
     5341 caaaggtaaa agaaaacctg tcccagaggt ttcccactgc ttgactttac ggaaaatttg
     5401 gttgataata ggactataac aaatatttag cactaggtaa taaagtctag taacaaaaga
     5461 actattacag cctgggaatc gtattggata tcccggggtt catacataat gatatataca
     5521 aaaggtgggg aaaaagtggc gcatccaata gtttccatag tcgggcgacc caatgtgggt
     5581 aagtccaccc tgtttaacca gctggttgga cggaggattt ctatcgtaga ttatcagccc
     5641 ggggtaacca gggacagggt atatgccgat acccagtggc tcaataccaa aatgaccctt
     5701 atagatacgg gtggaataga cacgaggacc caagacgagc tcatgacgca gatgaggcag
     5761 caggcagaca tcgccatcga tacagccgag gtgatcattt tcttggtgga cgggaaggaa
     5821 ggtataaccc ctgcagatca ggaggtggcg agcatcctaa ggagggcaca aaagcctatt
     5881 gtgttgggtg tgaataagat agatgccccc cacgaggagg gtatgctata tgagttctat
     5941 aacctgggga taggcgagcc catatccata tcggcgtccc ataaacgggg gcttggggat
     6001 ctgttggatg cggttgcaca gcaccttccc catgggtcgg atgaaaccga agacgacgag
     6061 acgataaaga tagcggttat gggcaagccc aacgtgggca aatcctccat agtaaataac
     6121 ctccttggcg aagaaagggt aatagtcagc gatattcccg gaactaccag ggatgccata
     6181 gatacaccct tcaccttaga cggacaaaaa tatgttatca tcgataccgc cggaatcaga
     6241 agaaagagca ggatagatga agacctggaa aggtacagcg taatcagggc cttagccgcc
     6301 atccgaaggt gcgacgttgc cctcatggtc attgacgcca cccaggaggt cacggaacag
     6361 gataccaaga tagcgggtct catccatgaa gagggcaagg ggattatctt agtcatgaac
     6421 aaatgggacc tgattgaaaa agagaccaat accatgaacc agtacaggcg gcggctgatc
     6481 aacgatttgg cttttatgca gtacgcgccc agcatcttta cttcggcctt aacagggcaa
     6541 aggatgggcg ggattgtggg tatggtaaat gaagtcttca gtcaatgcac ctttcgaatc
     6601 tctacaggcg tgctaaacga ctgtatcatg gatgcggtga ctttttcaga gccgcccacg
     6661 gataagggga aaaggcttaa gatctattac ggcactcagg tatccgtaaa gcctcccacc
     6721 ttcgtcctct ttgttaacga tgaaaagctc atgcatttct catatctcag gtatttggag
     6781 aatcatttca gaaagagttt tggactgcag ggaacaccaa taaggttcat tgtaaggcaa
     6841 aggaccaact aaagaagggg gcttagcaat gcggtatgtt cttatagttg tgatcggata
     6901 tattctgggc aatgtatcct cgtcctacct ggtaggaaag ctaggcagca atatcgatgt
     6961 cagacaacac ggcagcggaa atttgggtac caccaacgcc tatagggtct tggggataaa
     7021 ggcgggggct ctggtattta taggtgatgt aataaaaggg attttagcta ccatcatcgg
     7081 ccttctcata gtgaaagata gcagtgttgg cggccttata gggggcatag cggcagtcgc
     7141 gggacataac tggccgtttt tgttgggatt taaaggcgga aagggaatag ccacgtcctt
     7201 tggcaccata atcattctgt ttccccttac agccattatt ttgctggccg tagtggtgtt
     7261 gatcatcgtc ctgacggggt atgtgtccct agggtccata acagcggctg tattgttgcc
     7321 catcctggca attgctttta gatacaatcc atggcttata gttggggcat ttcttttagc
     7381 cgccctggcc atcctcagac ataaggccaa catatccagg ctcttaaagg gtacggagaa
     7441 taagataaca tctacaggcg gacctagggg actaaagtaa tcaatgaagt catccgctat
     7501 taagtagctc ataactatta tcatgcggca agataatgag ttagcatgat atggcaagtt
     7561 taattcaatg tgctagttaa atgtttctgt aatggctatt gaacgctact ttcaacattg
     7621 atttggagaa aactaaggct tattccattt gaaaggccta tcgcttccaa tccggcctcc
     7681 tgcccggtgg aatcggttta aggcgtcctg ccttaaacta cggcttttct gcattccata
     7741 aaccaagttt tcttagccca atcaatgaag tcatccgctt ttcaatagct cattactatt
     7801 ttcatgcggc agcattatga attagcccga catgataagt ttagttgacc tattgtttat
     7861 ctattattga tgacaacttg tcataaacct tttcccattc caatatac
//
LOCUS       DASRNX010000016         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_268487,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000016 DASRNX010000000
VERSION     DASRNX010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268487"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFD89_00590"
     CDS             complement(<1..270)
                     /locus_tag="VFD89_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56722.1"
                     /translation="MTKKAGRRFRPLLGLYQVWQICWYNFRRWRKNPRIIITFGLAFV
                     LCFLLTDKAVQFSYEYKTIMQVVEPFVWTFGDSNSILLASLLLILL"
     gene            complement(267..1016)
                     /locus_tag="VFD89_00595"
     CDS             complement(267..1016)
                     /locus_tag="VFD89_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56723.1"
                     /translation="MDEIKTDLKRAVYSGSFLLASIAMMLAIAIGAGGKLLFPKDIGM
                     GLDFDYHGQLIFTGLSSDIVLMVVPILCTLPYTAAFLDEYKSGFIKPYLMKCGKSAYI
                     KGKILGAAVSGGLALAIGIVVSYFITSLVYKPLGIADPTAVSSINVLFKRALIFFFCG
                     CLWASVGALLANITLSKYMAYGAPFVIYYVLVILSERYFHSIYVINPEEWLAPKNPWV
                     AGDWGIMLLVILVNIIVMMLNDVVIEGRIEG"
     gene            complement(1189..>2622)
                     /locus_tag="VFD89_00600"
     CDS             complement(1189..>2622)
                     /locus_tag="VFD89_00600"
                     /inference="COORDINATES: protein motif:HMM:NF039328.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6034 family protein"
                     /protein_id="HZJ56724.1"
                     /translation="SLIIALFLLITITACQKTPDSPIVIGKDIDNIIDKAVENPVVVD
                     KDSGEDLRKPLDAPEKLKWEAEGVKGNLKIYVDADVVVPNAASMPVARVTKGQFSEED
                     VKKLHEVFGGGAKIIVSDSPLPSSYYLEQVQDLKQRKEAGEYDDKFQDENIDDVINDL
                     LKQAAEAPETQEYEEIEPIFTFEENKDETQGPLGLYVHLKFMPNGNTISEINAIQDDY
                     GTGAYAEYIRDNTKKNEADSIATSYSDAYAGNVDIDILPTMKEEDAKELAQDTITKLG
                     LTDFVCSGQRLTPTNIPVYGEPMDQIRGVYEFMFTRKINDVPINYTNDEGLTTVHEAY
                     HAPWMYERIRIFIDDEGVFYLKWSSPYVVKEIVSDASTMLPFSDISDILKKMAPMKYD
                     YWDNDEIYSYEMKVTEARLGLMRVTEKDVGDSGLIIPVWDFFGTLTLKGKPGQPNPAE
                     GDQHYIYTSFLTINAIDGSIIDRDLGY"
BASE COUNT          736 a          614 c          509 g          763 t
ORIGIN      
        1 taggagaatg aggagcaaag aggccagaag tatggagttg ctatctccaa aggtccatac
       61 aaagggctct acaacctgca tgatggtctt atactcatag ctaaattgta ctgccttatc
      121 cgttaggaga aaacacaaca caaaggccag tccaaaggtg attataatac gagggttttt
      181 tctccatcgt ctaaagttat accagcatat ctgccacact tgatataatc ctagaagggg
      241 cctaaatctg cgtcctgctt tttttgtcat ccctcaatcc tcccctctat aactacatcg
      301 tttagcatca tcacgattat attaactaat atgaccaaca acataatacc ccaatcgccg
      361 gccacccaag ggttcttagg agctaaccat tcttccgggt tgattacata tatactatga
      421 aaatatcgct ccgaaaggat gaccagcaca taatagataa caaagggagc cccgtaggcc
      481 atatatttgc tcaaggtgat attggctagg agtgcaccga cagaggccca taggcatcca
      541 caaaaaaaga atatcagtgc tcttttaaac aaaacattaa tggacgatac agccgtggga
      601 tcagctatcc cgaggggctt gtagactagg gatgtaataa aataagagac tactatacca
      661 atggccagtg ctaaccctcc cgatacggct gccccaagaa tcttaccctt tatgtatgct
      721 gatttgccac acttcatcag ataaggcttg ataaatccac ttttatactc atcaagaaat
      781 gctgcagtat atggcagagt acagaggatg gggacaacca taaggacaat atctgaagat
      841 agcccggtaa agatgagctg cccgtgatag tcaaagtcta atcccatacc aatatctttg
      901 ggaaataaca gcttccctcc cgcacctata gcgatagcca acatcatcgc tatagatgcc
      961 agtagaaagg acccgctata tacggctctt tttagatctg ttttaatttc gtccacaata
     1021 cttatagtcc tttcgttatc tgttataaaa gtgatgatgt gggggaaatg tccccgtctt
     1081 tgtgaaataa tcactgtttg ggcaaataat ggcttaatca ctgtgatcgg catgcattaa
     1141 tgatgtgttc tccggctgac cgagacagta tgccaatgta agatgcattt aatagccaag
     1201 gtcccgatca atgatgctgc catctatggc attaatggtt aggaaagagg tgtatatgta
     1261 atgctgatca ccttcggctg gatttggctg cccgggtttc cccttaaggg tcaacgtccc
     1321 aaagaaatcc cacaccggga taatgagccc actgtctcct acatcctttt ccgtcactcg
     1381 cataaggcca aggcgagcct ccgtcacctt catctcgtag gaataaattt catcgttgtc
     1441 ccaataatcg tatttcatgg gcgccatctt ttttaatatg tccgagatat ctgaaaaagg
     1501 cagcattgtg gatgcgtcag agacaatttc tttcacgaca tagggtgaac tccactttag
     1561 atagaacaca ccttcgtcat caataaatat gcgtatcctc tcatacatcc aaggcgcatg
     1621 gtaggcttca tggacggtag ttagaccttc atcattggta tagttaattg gcacatcatt
     1681 aatcttacgg gtgaacataa actcatatac gcccctaatc tggtccattg gctccccata
     1741 cacgggtata ttggtcgggg ttaatctttg accggagcag acaaaatccg taagccccag
     1801 ctttgtaatg gtatcctggg ctagctcctt ggcatcttct tccttcattg tgggtaaaat
     1861 atctatgtct acgtttccag cataagcatc ggaataagaa gtggctatcg aatctgcttc
     1921 atttttcttc gtattatctc gtatatactc tgcatatgca cctgtgccat aatcatcttg
     1981 tattgcgttg atctcggata tagtattacc attaggcata aacttcaaat gaacgtataa
     2041 gcccaacggc ccttgggttt catctttgtt ttcctcaaaa gtaaaaatcg gctctatctc
     2101 ttcgtattcc tgggtttctg gtgcctctgc agcctgtttc aaaagatcat tgattacatc
     2161 gtcaatgttt tcatcctgaa acttatcatc atactcccca gcttcctttc gctgtttcag
     2221 gtcttgtacc tgctctaaat aataggaact gggaagaggt gaatccgaaa ctatgatctt
     2281 ggcaccaccg ccaaaaacct catggagctt ttttacatcc tcttcactaa actgaccctt
     2341 ggttaccctt gctacaggca tgcttgctgc atttgggaca actacatctg cgtctacata
     2401 gatttttaaa ttgcccttca cgccttctgc ttcccacttt agcttttcag gagcatcaag
     2461 gggctttcta aggtcctcac cggaatcctt atctactact acagggtttt ccactgcctt
     2521 atcaatgata ttatcaatat ccttacctat gacaatggga ctatccggcg ttttttggca
     2581 agcagttatt gttataagca gaaatagagc aatgattaaa ga
//
LOCUS       DASRNX010000017        11088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_533807,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000017 DASRNX010000000
VERSION     DASRNX010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11088
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533807"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFD89_00605"
     CDS             <1..269
                     /locus_tag="VFD89_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56725.1"
                     /translation="IEATNVELTEDDIQKQIKKLNGKGAVTMSILQKREEIGRRAGRR
                     EGRQEGLERVARNLLKEGMEIALVIKTTGLSKAEVEKLKEVLDS"
     gene            complement(390..466)
                     /locus_tag="VFD89_00610"
     tRNA            complement(390..466)
                     /locus_tag="VFD89_00610"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(430..432),aa:Pro,seq:ggg)
     gene            682..993
                     /gene="rplU"
                     /locus_tag="VFD89_00615"
     CDS             682..993
                     /gene="rplU"
                     /locus_tag="VFD89_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZJ56726.1"
                     /translation="MYAIIKTGGKQYLVHEGDVLRVEKLDVEEGSEVSFDEVLAINDD
                     ESLKVGTPIVEGSKVEAKVIKHGKGKKITVFKYKPKKNYRKKQGHRQPFTQVQISKII
                     G"
     gene            1007..1342
                     /locus_tag="VFD89_00620"
     CDS             1007..1342
                     /locus_tag="VFD89_00620"
                     /inference="COORDINATES: protein motif:HMM:NF016233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HZJ56727.1"
                     /translation="MVNILIVRNESGNVCRLKVAGHSGYDEIGRDIVCSAISAIVQTM
                     VIGLKDVVGIRPLYHQKSGLADLKIPDDLSEEKIKKANILLETGLLGLRSIESGYGEY
                     VDIRERKVE"
     gene            1343..1639
                     /gene="rpmA"
                     /locus_tag="VFD89_00625"
     CDS             1343..1639
                     /gene="rpmA"
                     /locus_tag="VFD89_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZJ56728.1"
                     /translation="MFVMNLQLFASKKGVGSSRNGRDSEAKRLGVKRTDGQFVLAGNI
                     LVRQRGTRLHPGNNVGRGKDDTLFALVDGVVKFERRGRDRKQVSVYQEAQLAVH"
     gene            1968..3239
                     /gene="obgE"
                     /locus_tag="VFD89_00630"
     CDS             1968..3239
                     /gene="obgE"
                     /locus_tag="VFD89_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZJ56729.1"
                     /translation="MFVDIVKVYIKAGDGGNGAVSFRREKYVPAGGPDGGDGGNGGDV
                     VFVVNTGKHTLMDFRYKKSFKAQSGEDGRGRKMHGKKGEDVIIEVPPGTIIKDSETDR
                     VIADLTEPAQREVLVRGGRGGRGNARFATATRQAPRFARKGLKGQELWVTLELKSIAD
                     VGLIGFPNVGKSTILSVLTASRPKIANYHFTTLSPNLGVVDTRFDKSFVMADIPGIIE
                     GAHEGTGLGIDFLRHIERTRILVHVLDASGIEGRDPIDDFHTINKELESFSKELARRP
                     QIVAANKTDIPTAGENIVRLEEELEGKDMKIFPVSAATGKGFDPLVSELVNMLEQLPP
                     LEPFEPEFAWEDEDVGEGTFEIEIVDDIYTVYGPSIDGLLGRVNLEDYESMQYFQRTL
                     RRMGIIDALKSAGIEDGDTVQINDLEFDFIE"
     gene            3281..3574
                     /gene="yhbY"
                     /locus_tag="VFD89_00635"
     CDS             3281..3574
                     /gene="yhbY"
                     /locus_tag="VFD89_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HZJ56730.1"
                     /translation="MLTSKQRSYLRGMANQTDSIFQIGKGGINANLIKQLDDALEARE
                     LIKVHVLQNAESDIKDIFKRLAQELSAHEVQLIGSRFVLYRKSAKNPKIELPY"
     gene            complement(3568..4485)
                     /locus_tag="VFD89_00640"
     CDS             complement(3568..4485)
                     /locus_tag="VFD89_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56731.1"
                     /translation="MSFKTYIQKARSLSYYMGDEDTPRPVIGLIADFVLPIIGFSFVL
                     YLWLVFRTRSSLLSLIIGLGVSSLFAYALYLRKRASYRTRRSRMRRVIAREYMAGELS
                     VLSRQEFEWQLVKALSALKGLKDIDQRQGHLKAQYNNMPIAIGYHHAPPKGYESYERV
                     WTFYNAYRSRGYDSLIYISSGYFEDACKYIQDGNLQIPITLMDVESLLDLMEDAGMTP
                     GDDHLDHLVTERIAEIKQKGRHSKNRIATQYKVKRYMMASLLFLGVSLLFRKYFLFYF
                     VLSALFFVLGLISQILSGNEKESDLKSPQ"
     gene            4886..5500
                     /gene="nadD"
                     /locus_tag="VFD89_00645"
     CDS             4886..5500
                     /gene="nadD"
                     /locus_tag="VFD89_00645"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZJ56732.1"
                     /translation="MKKLGVYGGTFDPIHMGHLIVAEMARQECGLDRVLFIPAKIPPH
                     KNDNHISSPDHRFEMTSLAIGGNPSFDVSDIELKRQGISYTVDSIKILNRGNPNGTKI
                     WMIVGGDSFLEMDSWKDAGTIMAMCNFAVYPRPGFPIDALKAKGKSVSKHFDADIHFV
                     QAPMIDISSTDIRNMVSKGKSIRYMVPDSVREYIMDKELFATNG"
     gene            5614..6207
                     /gene="yqeK"
                     /locus_tag="VFD89_00650"
     CDS             5614..6207
                     /gene="yqeK"
                     /locus_tag="VFD89_00650"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HZJ56733.1"
                     /translation="MDKKQIIDRLSEMLEPKRLQHSIRVMECAERLARLHGVDEDKAV
                     IAGLLHDCAKGFSGEALIERSKEYGIELDEISLYQRGLIHGPLGAGVASRVFGIEDLD
                     ILSAIEHHTYGKKDMDELEMVIYLADLVEPGRTFEGVDELRALASEDLEGAMLKALDD
                     IIALVMAKGGLIHPNTLEARNSIILNKLRFGEEICCH"
     gene            6198..6551
                     /gene="rsfS"
                     /locus_tag="VFD89_00655"
     CDS             6198..6551
                     /gene="rsfS"
                     /locus_tag="VFD89_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZJ56734.1"
                     /translation="MPLNSKRTALKIAEILRDKKAQDVTVLDIHSLTIIADYFIIASG
                     RSESQVKALYEEVEDQMKKEGLESRHRDGQKGNRWVVLDYGDVILHVFHHEEREFYGL
                     ERLWADGISLSVDNP"
     gene            7022..9499
                     /gene="leuS"
                     /locus_tag="VFD89_00660"
     CDS             7022..9499
                     /gene="leuS"
                     /locus_tag="VFD89_00660"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZJ56735.1"
                     /translation="MKYEFTKIETKWQKRWEAEGDFRKVEDPSKEKYYMLEMFPYPSG
                     NLHMGHVRNYSIGDVIARFKTMEGYNVIHPMGWDSFGLPAENAAIERDIHPDEWTRSN
                     IDTMREQLKGLGFSYDWNREVATSSPDYYKWTQWLFLQFFKKGMAYKKKSSVNWCPSC
                     ETVLANEQVVNGGCERCSTEVDKKELEQWFFRITEYAQRLLDDIERLEDWPEKVRVMQ
                     QNWIGRSEGAMVSFQIHEHDEKIEVFTTRPDTIYGVSSMVLAPEHPLVPELVEGTEYE
                     EPVNQFRKRMEHLSEITRTSTEAEKEGLFIGRYVINPMDGNKIPMFIANYVLMDYGTG
                     AVMGVPAHDQRDFEFATKYDLPIIPVIKPRDEGIDIENLNQAFEAEGVMINSGPFNGL
                     DSNQGISKVIEYMEDKSIGEGKINFRLRDWLISRQRYWGAPIPILYCDKCGMVPVPEE
                     DLPVLLPTDAKLSGKGNSPLSTSDSFVHTTCPRCGGDARREVDTMDTFVCSSFYFLRF
                     ADPQNHNQPFGLDKTKYWMPVDQYVGGVEHAILHLLYARFFTKVLFDMGLSPVEEPFS
                     RLLTQGMVLKDGVKMSKSKGNTVSPEEIIVKYGADTARLFILFGAPPERDLEWSDQGV
                     EGSFRFLNRVWRLVTGSYREAVDNGLGNESSVTDRDKKLRFVLHNTIKRVTTDIGERF
                     NFNTAISAIMELVNAIYDYLEKNKDGNFNKDALTNAITDMIIMLTPFSPHIASELWEF
                     IGKEGSVHDEVWPKWDPKALIKDEIEIVVQINGRLRERLIISADATREKMQETALEQD
                     KIKELIAGKSIVKVIVVPKKLVNIVVR"
     gene            complement(9597..11048)
                     /locus_tag="VFD89_00665"
     CDS             complement(9597..11048)
                     /locus_tag="VFD89_00665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZJ56736.1"
                     /translation="MKRITVMFLVLICLIAFANPIQATPDGDTVDINILSNITSEKIE
                     LDAQGAVLMALDNGEILFSKDENKRLYPASTTKILTGLLALEYGDLEEIVTVGNEANL
                     CAYDSSIAGVDIGEEISLENLLYGLMLPSGNDAAYVIAVHIGRKIKGDSNVSIDEALG
                     AFVNLMNRRSRELGVAHSNFMNPDGYHHDDHYTTARDLALITREAMNHDVFRQIISTE
                     MFTIPDWSSMHDPKAEEKEIRYWRNTNALIQPKNKFYYPDATGGKTGYTSNARHCLVA
                     IASRDGTGLLTVVLGNTTKDSKWIDSVMLLDYGYDNFVRYQPFSQDEQVHILDITGNP
                     DPDWVRVLMDKTPTRFVSRDQIGKISKEIQWEPEVAVNVEDNRNTISLPGPVSRRQKV
                     GTMIFRLGNTVLDRVDLVTAKAVEIRKPEKSKFPVKLRDGVEASYLTLWYNLSIWQKF
                     ALGSGIFVLVIFALAMRTIAVKRRRGRYVFRRR"
BASE COUNT         3309 a         2120 c         2801 g         2858 t
ORIGIN      
        1 atatagaggc gacaaatgtg gaactaacag aagatgacat tcaaaaacaa ataaaaaaat
       61 taaacggaaa gggggctgtt accatgagta tattgcagaa aagagaagag ataggcagac
      121 gggcaggaag acgggaaggc aggcaggaag gcttagagcg agtggccagg aacttattaa
      181 aagaaggaat ggaaatagct ttagttataa agactacagg cctctctaaa gccgaagtag
      241 aaaaattaaa agaagtgttg gatagttagc tgcacatatt gcgataggaa ataaaggccc
      301 tgtcgcactc ttgattttta gggctgattt ataaaggact tcccaaaaaa acaaaaaccc
      361 gctcatcctt acggactagc gggtttcact ggtctgggtg acaggaattg aacctgcgac
      421 ctcttgaacc ccattcaagc acgctaccaa actgcgccac acccagaagt ttatgagaac
      481 aaataatata atactataga atttagaaaa aatcaagtgg aatttttgtt tgacaatcaa
      541 cttatcattg tgataaaata ttttagtgcg tagccgcacg gatatggtta taaatcctta
      601 ttgtgaaacc attgtatggt tttctttgaa aatcaatagg taccactatc cggcgagact
      661 ggttagagga ggtgcatcgt aatgtatgct atcattaaaa cgggtgggaa gcaatacctg
      721 gtccatgaag gtgatgtttt aagggttgaa aagctggacg tagaagaggg ttcggaagta
      781 tcctttgacg aggtactggc tatcaacgac gatgagtctt taaaggttgg aacccctatc
      841 gtagagggat ccaaagttga agccaaagtc ataaaacatg gaaaaggcaa gaaaataacc
      901 gtttttaagt acaagcccaa aaagaactac aggaaaaagc agggacaccg tcagcctttt
      961 acccaagttc aaattagtaa aattatcgga taattgtggg aatgaaatgg ttaatatatt
     1021 gattgtacgg aatgagtcgg gtaatgtctg tcggttaaaa gtagccggtc attcagggta
     1081 tgatgagata ggacgggaca ttgtatgcag tgctatatcg gctatagtcc agacaatggt
     1141 cattggactt aaggatgtgg tgggtataag acccctatat catcaaaaaa gcggtttagc
     1201 cgatttgaag atccccgatg atctttctga ggaaaagatt aagaaggcaa atatattact
     1261 ggaaacgggc ctgttggggc tcaggagtat agaatcagga tatggtgaat atgtagatat
     1321 tcgggaaagg aaggtggaat agatgtttgt aatgaatctt cagctctttg catccaaaaa
     1381 gggagtggga agttcccgaa acggacgtga cagtgaggct aagagattag gagttaaaag
     1441 gacagacgga caattcgttt tggccggaaa catccttgtg agacagagag gaaccagact
     1501 tcatcccggt aacaacgtgg gtaggggtaa ggacgatacc ctctttgcat tggtagacgg
     1561 tgtagtcaag tttgaaagaa gaggccgtga tagaaagcag gttagcgtct atcaagaggc
     1621 acagctggct gtacactaag aatatctgtt ttatggactt atgatcgggt ctattacatg
     1681 agattgctat gtgatagacc ttttctatat ttatgggtat attaaatact ggatggggat
     1741 ttccttaaga cattgatgct ctgggacaaa gttcgaaagg gctcggaagg gctcggaagg
     1801 actcgggatc taaaattatt gaagttttga ttgggaattg ttataattca taattaggaa
     1861 aacaatagag gatgccagat tgcggtattg tactctaccg tttcatatgg cggaataaaa
     1921 aactagacga aaattgtttt cccataacat gaggagcaat agattatatg tttgttgata
     1981 ttgtaaaagt ttacataaag gccggagacg gcggcaacgg cgccgtttcc tttagaaggg
     2041 aaaagtatgt tcccgccggt ggacctgatg gcggggacgg cggcaacggc ggcgatgttg
     2101 tatttgtcgt aaacacgggg aagcacaccc tgatggattt tcgatataaa aaatccttta
     2161 aggcccagtc cggagaggat gggcgcggaa gaaagatgca tggtaaaaag ggtgaggatg
     2221 taataataga agtacccccc ggaaccatta tcaaagatag tgagaccgat agggtaattg
     2281 cggatttaac cgaacctgcc caacgcgaag tattggtccg cgggggtaga gggggcaggg
     2341 gtaatgccag gtttgctacc gctacccgcc aagctccaag gtttgcaagg aaggggctaa
     2401 aggggcaaga gctctgggtt accctagaac taaaatccat tgccgacgtt ggactgatcg
     2461 ggtttcccaa tgtaggaaaa tctacgattt tatcggtgct gactgcgtct cggcccaaga
     2521 tagcgaatta tcatttcacc accctttcac cgaatcttgg agtcgtggat acgagatttg
     2581 ataaatcctt tgtcatggct gatatccccg gtataataga gggggcacat gaaggaacag
     2641 ggctggggat tgacttttta aggcatatcg aaaggacaag gatattggtt cacgtgctag
     2701 acgcttccgg catagaggga agggacccca tagatgactt tcataccata aataaggaat
     2761 tagaatcctt tagtaaggaa ctggcccgaa gacctcaaat agtggcagcc aataagacgg
     2821 atatacccac tgcgggggag aacatcgtca ggttggaaga ggagcttgag ggaaaggata
     2881 tgaagatatt ccctgtatcg gcggccaccg ggaagggctt tgatccccta gtatcggaac
     2941 tggtgaacat gttagagcaa ctcccgcccc tagagccctt tgagccagag tttgcttggg
     3001 aagatgagga tgtcggggaa ggaacctttg aaattgaaat tgtggacgat atatataccg
     3061 tatacggacc atcaattgat ggattgttgg gaagagtgaa cttggaggat tacgagtcca
     3121 tgcaatactt tcagaggacc ctcagaagga tgggaatcat cgatgcctta aagagtgcag
     3181 gcatcgaaga cggtgatacg gtacagatta atgaccttga atttgacttt atagaataaa
     3241 gggattacca tatattggtg ataaaaaagg ggtgaacagg atgttaacca gcaagcaaag
     3301 aagttatctg aggggaatgg ccaatcaaac cgatagcatt ttccaaatag gtaagggcgg
     3361 gataaacgcc aatttgataa agcagttaga cgatgctctt gaggccaggg agcttataaa
     3421 ggtgcatgtt ttacaaaatg ccgaatcgga tataaaggat atctttaaga gattggccca
     3481 agagctgtca gcccacgagg ttcaactcat cggcagcagg tttgtactct acagaaaatc
     3541 tgcgaagaat cccaaaattg aactccccta ttgagggctc tttaaatcgc tctctttttc
     3601 gttgccggac aggatctggc taatcaatcc caatacaaag aacagagcgg acaaaacgaa
     3661 atagaagaga aagtatttgc gaaacagcag ggatacgcct aaaaacaaaa gggatgccat
     3721 catatatctc ttaaccttgt actgcgtcgc gatccggttt ttgctatgcc ttcctttttg
     3781 tttaatctct gcaattcttt ccgtcacaag gtgatctaga tgatcgtctc cgggggtcat
     3841 acctgcatcc tccataagat caagcagact ctccacatcc attagcgtga tggggatttg
     3901 gagattcccg tcttgtatgt atttgcaggc atcctcaaaa tagcccgatg agatatagat
     3961 gagactgtcg tatcccctag atcgatatgc attatagaaa gtccacaccc tttcgtagga
     4021 ttcataaccc ttaggggggg catgatggta gcctatggct atgggcatat tgttatattg
     4081 ggctttgaga tgcccctgcc tctggtcgat gtcctttaat cccttaaggg cggataaagc
     4141 tttgacgagc tgccattcaa attcttgtct gcttaggacg gatagctcac cggccatata
     4201 ttccctggct atgactctgc gcattcggct tcgccttgtt ctgtaggatg ccctttttcg
     4261 aagataaagt gcgtaggcga aaagggatga aaccccaagg ccgataatta atgacaatag
     4321 actggatctg gtacggaaaa ccaaccatag gtataagaca aaggaaaaac cgataatggg
     4381 caatacgaag tctgcaataa gccctataac ggggcgtggg gtatcttcgt cacccatata
     4441 ataactgagg gagcgggctt tttgtatata agttttaaag gacattataa acacctccaa
     4501 tagtattatt gccatgtttt gggggattaa aaccgcagtc ccctttgttt tacgcataaa
     4561 attagcctat tgcgtcggtt tttttatctt gatgagtggt aaaggtaatg acctattggt
     4621 aaatgggcga tgtcattggt tgggattctg aaagccggcc gaaagaaact ctaattttgc
     4681 gacttagacc cagggcatgg tttggcataa ttccggtata aaatatttca gaaattctct
     4741 gtcacttaac gttacaaccc ggttacgggg tgtttatagt caaacctata gtaccggggc
     4801 ctgaaatatg gtataattat atacgttaag aaaaagactt tttactacag gagactcatg
     4861 ctttaaggag aataatgaaa aattggtgaa aaaattagga gtatatggcg ggacctttga
     4921 tccaatacat atgggccatt taattgtggc agagatggcc aggcaggagt gcggtcttga
     4981 cagggtactc tttatcccgg cgaagatacc gccccataaa aatgataacc atattagttc
     5041 cccggatcat cgttttgaga tgactagcct tgcgattggg ggaaatccta gctttgatgt
     5101 aagcgatata gagttgaaga ggcaaggtat aagctataca gtagatagca ttaagatttt
     5161 gaaccgaggg aatcctaatg ggaccaaaat ttggatgata gtaggcgggg attcattttt
     5221 agagatggac agctggaaag atgcagggac aataatggcg atgtgtaact ttgctgtata
     5281 tcccaggccc ggctttccga tagatgcgtt gaaagctaag ggaaaatctg tcagcaaaca
     5341 ttttgacgcc gatatacact tcgtacaagc gcctatgatc gatatatcat cgacggatat
     5401 aagaaatatg gtgtcgaagg gaaaatccat cagatacatg gtccctgatt cggtgaggga
     5461 gtatattatg gataaggaac tctttgctac gaatgggtag ccgatatggg gagatgggtg
     5521 atatcccgcg acggccaagg catggtgtag ggtgtttgta atgggatgta tagtcaggca
     5581 ccatcttggt aatacgtaaa aatggagtat ctaatggaca aaaagcaaat tattgataga
     5641 ttgtctgaaa tgttggaacc taagaggctg caacattcca taagggtgat ggaatgtgcc
     5701 gaaaggttgg cacgcctcca tggagtcgat gaggacaaag cggtaatagc tggcttgttg
     5761 cacgattgtg ccaagggatt cagcggtgag gcgctaatag aaagatcaaa ggaatacgga
     5821 attgagttgg atgaaatcag cctttaccaa agaggtttga ttcacggccc gctgggtgcc
     5881 ggggtagcaa gccgcgtttt tggcatagag gatttggata tcctgtcagc cattgaacat
     5941 cacacatatg gcaaaaaaga tatggatgag ctggagatgg tcatatactt ggccgatctg
     6001 gtggaacccg gtcgtacctt tgaaggcgta gacgaattga gagcccttgc gtcggaggat
     6061 ttagaaggtg cgatgctaaa ggctctggat gacataattg cgttggttat ggccaagggc
     6121 ggactaattc atccgaatac cctggaagcg cgcaatagta taatattaaa taaattaaga
     6181 tttggggagg agatttgttg ccactaaatt caaagcgcac tgcgcttaag atcgctgaga
     6241 tattacggga taaaaaagcg caggatgtca cggtcttaga catccattcc ctgaccatta
     6301 ttgcggatta ttttatcatc gcaagcggtc gatcagaatc tcaggtgaaa gccctttacg
     6361 aggaagtgga agatcagatg aaaaaagagg gtttggaatc cagacaccga gacggccaga
     6421 agggtaaccg atgggttgtt ttggactatg gggatgtcat cctccatgta tttcaccatg
     6481 aagagaggga attttatggc ctggaacgcc tatgggctga tggcatatcg ctatctgttg
     6541 acaaccctta attgatgcaa tataattatt gtcatccata aaggattatt gatctacttt
     6601 agctactgta atcagtgtca gaacgaacct gatgaaagta tacatggctt tattgctttt
     6661 aaaattcata aagattgcac cgggagtagt aggataccgg aaactttcag agaatcgacg
     6721 gatggtgtaa gtcgatattt gatggattcg aactcgccca aatcaggttg caggggttca
     6781 tcgccttatg gatgaagagt ggatgctata gttaatttta ctcgattacc ccatgggata
     6841 ttgttaattt ctagccgaac ttatcctgcg ggctataatc ataaacttac tatgcaatca
     6901 aatagggtgg taccgcgggt aacctctcgt ccctaacgga gcgaggggtt tttaatttcc
     6961 aaaaaccggg taaccggatt ccggtaaagt gtttgttgta gggtatatag gaggtatagg
     7021 tatgaaatat gaatttacta aaattgaaac aaagtggcag aagagatggg aagctgaagg
     7081 agacttccga aaggtggagg atccaagcaa ggagaaatac tacatgctgg aaatgtttcc
     7141 ctacccgtcg ggcaacctgc atatggggca tgtgcgaaat tattccatag gcgatgtcat
     7201 agcgcggttt aagaccatgg agggttataa tgttatccat cccatgggct gggactcctt
     7261 cggcttgccg gcagagaacg ccgctataga acgggatatt cacccggatg aatggacccg
     7321 gagtaatatt gatactatga gggagcagct caagggtctg ggatttagct acgactggaa
     7381 cagggaagtc gccacctcca gccctgatta ttacaaatgg acccaatggt tattcctgca
     7441 attctttaag aagggaatgg cttataagaa gaaatcatct gtcaattggt gtccttcctg
     7501 tgaaacagtg ctggccaatg agcaggtggt aaatggcggc tgtgagagat gcagtactga
     7561 ggtggataaa aaggaactgg agcaatggtt tttcaggata actgaatatg cccagaggct
     7621 tttagacgat attgaaaggc tggaggactg gcccgagaag gtcagagtta tgcagcaaaa
     7681 ttggattggt agaagtgaag gggcaatggt atcctttcag attcacgaac acgacgagaa
     7741 gatcgaggtg ttcacaacca ggccggatac catatatgga gtatcctcta tggtattggc
     7801 ccccgagcat cccctggttc cggagttggt agagggaacc gaatatgagg agcctgtcaa
     7861 ccagtttaga aaaaggatgg agcatctcag cgagataacg aggacatcca ccgaggcgga
     7921 aaaagagggg ctctttatag gcagatatgt cataaatccc atggatggga acaaaatccc
     7981 tatgtttata gccaattatg tattgatgga ctatggaacg ggggcggtaa tgggggttcc
     8041 tgcccatgac caaagggact ttgaatttgc taccaagtac gatcttccca tcattccggt
     8101 aatcaagccc agagatgaag gcatcgatat tgaaaatctt aatcaggcct ttgaagcaga
     8161 aggcgttatg ataaattccg gtccgttcaa cggccttgac agcaaccagg gaatctccaa
     8221 ggtgatagaa tatatggagg ataagagtat tggggaggga aagatcaact ttaggctcag
     8281 ggactggctt atatcccgcc aaagatattg gggggcccct attcccatcc tatattgtga
     8341 taaatgcggg atggtaccgg tgccggaaga ggatctcccg gtattattac ccacggatgc
     8401 aaagctttcc ggtaagggaa attcgcccct cagtaccagc gactcctttg ttcatacgac
     8461 atgcccaagg tgtggcggag atgccaggcg agaagttgat acaatggata cctttgtgtg
     8521 ttcatccttc tacttcctaa ggtttgcgga tccacaaaat cataaccagc cctttggact
     8581 ggataaaacc aagtattgga tgcctgtaga tcagtatgta ggcggggtag aacacgctat
     8641 ccttcatttg ctttacgccc ggttcttcac caaggtgctc tttgatatgg gtctatcccc
     8701 tgtagaagaa cccttttcca ggcttttaac ccaaggtatg gtcctaaagg acggggtgaa
     8761 aatgtccaaa tccaagggta ataccgtaag ccctgaggag attattgtaa aatacggggc
     8821 ggataccgct cgtcttttca ttctcttcgg cgctcctccg gaaagggact tggaatggag
     8881 cgatcaaggt gtggaaggca gttttagatt cctaaatcgg gtctggaggc tggtgacggg
     8941 atcctatagg gaagccgtcg acaacggact ggggaatgag agcagcgtta ctgataggga
     9001 caagaaatta agatttgtgc tccataatac aatcaagagg gttactacgg atatcggcga
     9061 acggttcaat tttaatacgg cgataagtgc tattatggaa ctggtgaatg caatttatga
     9121 ttacttggaa aaaaataaag acggtaattt taataaggat gcgctgacga acgccattac
     9181 cgacatgata attatgctga ccccattttc cccccatata gcttcggaat tgtgggaatt
     9241 tattggaaag gaaggcagcg ttcacgatga ggtatggccg aaatgggatc caaaagcact
     9301 gattaaggat gagatagaga tagtggtaca aataaatgga aggctcagag aacgcctaat
     9361 aatatcagcc gatgctacca gggaaaaaat gcaggaaact gcgttagaac aagacaagat
     9421 caaagaattg attgcgggta agagcatagt aaaggtaata gtagtaccca aaaaactggt
     9481 caatatcgta gtcagatgag atttataccg gatatataat tctaaaaagc agtggatgcg
     9541 actggaacct attgaggact tgcatgtaaa tcagattgaa gatagccgtt tgtcggttat
     9601 cttcttctga atacatacct tcccctcctt cgttttacgg ctatggttct catggctaaa
     9661 gcaaagatga ccagaacaaa tattccggac ccaagggcaa acttctgcca tatactcaga
     9721 ttgtaccata gggtcagata tgaggcctcc acaccgtccc gtagtttaac ggggaatttg
     9781 gatttttcag gctttcgaat ctcaacagcc ttcgcagtta ctaaatcaac cctgtcaagg
     9841 accgtattcc caagccggaa tatcatagtc cccacttttt gacgccggga aaccggacca
     9901 ggtaaggaga tcgtattcct attgtcctcc acattcacag ctacttcagg ctcccattga
     9961 atttccttgc tgatcttgcc tatctgatct ctagatacga aacgtgtcgg cgtcttatcc
    10021 attaaaaccc taacccaatc aggatcaggg ttacccgtta tatccaaaat gtgcacctgc
    10081 tcatcctggg aaaagggttg gtaccttacg aaattgtcat acccataatc caacagcata
    10141 acagaatcaa tccatttact gtccttagta gtattaccta aaacaacggt caacagcccg
    10201 gtgccatcac gggaggctat agccaccaga caatgccggg catttgatgt ataaccggtc
    10261 tttccaccgg tggcatctgg ataatagaat ttattcttag gttgtatcaa tgcgttggta
    10321 tttctccaat acctgatttc cttctcctct gctttgggat catgcatgct ggaccaatcg
    10381 ggtatggtaa acatttctgt gctgattatc tggcgaaaaa cgtcatgatt catggcctct
    10441 ctggtaataa gggccaaatc acgggccgta gtataatggt cgtcatggtg ataaccgtca
    10501 ggattcataa agtttgaatg ggcgaccccc agttccctgg agcgtctatt catcaggttc
    10561 acaaatgccc ccagggcctc atcgatggat acattcgaat cgcctttgat cttgcgcccg
    10621 atatgtaccg caatgacata agctgcgtcg tttcctgagg gaagcatcaa gccgtatagc
    10681 agattttcaa gggaaatttc ttcgccaata tcgacgcccg ctatgctgct gtcataagcg
    10741 catagattcg cttcatttcc gactgttacg atttcttcca gatccccata ttccaaagcc
    10801 aataacccag ttaatatctt cgtagtgctg gccggataaa ggcgtttatt ctcatccttg
    10861 gaaaagagta tttctccgtt atccaaggcc attaaaacag cgccctgggc atcaagctca
    10921 attttttccg atgttatatt agaaaggatg ttgatatcaa ccgtatcccc atccggtgta
    10981 gcttgtattg ggttggcaaa ggcaatcaaa caaattaaga ccaaaaacat taccgttatt
    11041 cttttcatct taaccccaca ccctttagga caaatataaa gtcaattc
//
LOCUS       DASRNX010000018         8117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_692992,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000018 DASRNX010000000
VERSION     DASRNX010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692992"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VFD89_00670"
     CDS             <1..764
                     /locus_tag="VFD89_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZJ56737.1"
                     /translation="ILLCNTMNDNIITNTNVEMNYIDFILEKQVDGLIIMGGHIDNHF
                     IAPEYLERINTLTEQIPVVMINGEIRGLDCYNIRNDSSIGINQLVDYLVSLNHKKIAF
                     IGGYTDIQPTMRRLKIIKESLNRYGIGFNGEWFIEDDFSVQAGKDSMEKLLYMKDKPT
                     AVICVNDMVAVGALHTATKMGVKVPDHISIAGFDNTYLTECVVPEITTVDLRPKRLAR
                     TAVETMVGHLEGVRVDKEITIKTQLVLRDSCSIAD"
     gene            899..1036
                     /locus_tag="VFD89_00675"
     CDS             899..1036
                     /locus_tag="VFD89_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56738.1"
                     /translation="MIVKIRDIVKIRDDVKGIHGLLAVEQIPDNHGLPMSLGIGEKLS
                     A"
     gene            1189..2997
                     /locus_tag="VFD89_00680"
     CDS             1189..2997
                     /locus_tag="VFD89_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ56739.1"
                     /translation="MEDKKWSYLWKITKGNRMLYVGAIISMALSAVLSLFPPLVISIT
                     VDSIIGDEPLIDSPVVVSMIQRLGGKGVLAQNLWICGLVLVILNIFIGYFNFTRGKWS
                     AQAAEGIARGIRNNLYDHLQHLPYDEHVKAETGDLIQRCTSDVETVRRFLAMQLVEMG
                     RAIVLLVASLFFMFSLNVKMTFVSIAVVPFIFLFSFIFFSKIKEAFKEADEAEGRLST
                     VLQENLTGIRVVRAFGRQAYEADKFDEKNTEYTNLWYRLNSLMALYWSSSDVLAMGQI
                     GAVLVLGVYWAAQGELTLGTLLAFMSYEWMLLWPVRQLGRILTDMGKTQVSLGRIKEI
                     IEKPLEDMNEDGLTPPMDGDIEFKNVYFEYEKGRPVLKDVSFRVEGGKTIAILGSTGS
                     GKSSLVHLLQGLYDYDRGSITIGGVELRDIEKRYLRKQVGIVLQEPFLFSKTIKDNIA
                     LAKVGASDNEIHNAANTAAVHDVIREFEKGYDTTVGERGVTLSGGQKQRVAMARTLIL
                     DSSILIFDDSLSAVDTETDNAIRSALNERSRDVTTFIISHRITTISQADVILVMEDGR
                     IVQSGIHDELLSQEGMYRRIWSIQNALEEELEKNLA"
     gene            3081..4907
                     /locus_tag="VFD89_00685"
     CDS             3081..4907
                     /locus_tag="VFD89_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ56740.1"
                     /translation="MNELKEQEYNKSIDFGLWKDLFRYIKPYRKRIIALIVVMMGSGG
                     IDVIFPLMNRYAIDNFIMTGRLEGLWVFAGIYGGLVAIQAINVYFFIYIAGRIELNLS
                     YDIRKVGFKRLQELSFSYYDKTSVGWLMARMTSDVERLGATIAWSLVDLTWGGTAMVG
                     GLIAMLYMNWKLALISLAVVPFLAVISIFFQKRILGSYRIVRRTNSRITGAFNEGIMG
                     AKTTKTLVREKENLKEFKGLTGEMYSSSVRAAVFSSVYFPIVITMGSVGTALALWFGG
                     RGVLLQAIGFGTLSAFISYVMQFFGPINELARIIAEFQNAQASAERVMAMINTEPDIV
                     DTAEVIEKYGTEFKPRYENWEVIDGGITFSDVSFSYKEGEEVLEDFSLDVQRGESIAL
                     VGETGSGKSTIVNLICRFYEPVEGQILIDGVDYRERSQLWLQSNIGYVLQTPHLFSGT
                     VNENIRYGRLDATDEEVEEAARLVNAHNFIMKMEDGYDSEVGEGGNRLSTGEKQLISF
                     ARAILADPRILVLDEATSSIDTETEQMIQYAINKLLEGRTSFIIAHRLSTIRSADRIL
                     VIKDGRMIEQGNHRELMGLGGYYYRLYTNQFREEQELSAINA"
     gene            5040..5582
                     /locus_tag="VFD89_00690"
     CDS             5040..5582
                     /locus_tag="VFD89_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZJ56741.1"
                     /translation="MRELWELFSVFFRIGAFTFGGGYAMLPIIQREVVEKREWATDEE
                     VIDYYAIGQSTPGIIAVNTAIFIGYKNKGIIGGIFATAGMVFPSLVIIMAIASSFMHF
                     QENLIVQHAFAGVRVAVVALVVNAVVRMWKTSVKDVVGIILFTVAFLVIAFTRISPII
                     LIFASAVTGIILTSRKAVDK"
     gene            5579..6145
                     /locus_tag="VFD89_00695"
     CDS             5579..6145
                     /locus_tag="VFD89_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZJ56742.1"
                     /translation="MIYLYLFFEFFKVGLFAVGGGLATIPFLQELIQKYGWVTPQELI
                     NMIAISESTPGPIGVNMATYVGFETAGIGGAILATVGLVTPSVIIIVIIAHYFMRFAE
                     EPLVKAGFYGLRPAVTGLIAAAGFEVARVSLFNLQRYLETKKVLDILDPKAIVLFGIL
                     MYVTNKYKKHPIIYLVAAGVVGVIFKFG"
     gene            6535..7179
                     /locus_tag="VFD89_00700"
     CDS             6535..7179
                     /locus_tag="VFD89_00700"
                     /inference="COORDINATES: protein motif:HMM:NF027338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HZJ56743.1"
                     /translation="MGRVCEWKKVRDDEVNPLIWEQIDPVLIEEYPWDENGYKPRVEV
                     RFFYTESRLHLQFTSYESEIRAVYRNMNEPVYTDSCVEFFFNPDPHRDDRYFNFEINA
                     IGTLLLELGNDRNNRLMIRDTPQDIFCIKSSVSPDNADEYEGDSWILELSIPYSFIER
                     YYGSQDFSPGKRMRGNFQKCGDKTKFPHHGSWNRIDTLEADFHRPEYFGDLILK"
     gene            complement(7286..7819)
                     /locus_tag="VFD89_00705"
     CDS             complement(7286..7819)
                     /locus_tag="VFD89_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HZJ56744.1"
                     /translation="MIIIRDSLFRNSMHKNLTLDQVYEMLVQFLRQAPHNEYRLSIGT
                     DSQVGAHETVFVTAIHLHKVGRGATGFFTTQFFPRPIKSLREKIYYETSKTLELAAWF
                     TDEKMDKLVDCILTTMGRPGDIHFEFHLDVGTVGPTRELISEMVAMANGTGFEPKIKP
                     ESYAASCYANRYTKKIS"
BASE COUNT         2244 a         1612 c         2076 g         2185 t
ORIGIN      
        1 gtatattgct atgcaatact atgaatgaca acataataac caatacgaat gtggaaatga
       61 actatataga tttcatttta gaaaaacagg ttgacggctt aattattatg ggcggtcata
      121 tcgacaacca ttttatcgcg ccggaatact tagagaggat taatacgctg accgaacaga
      181 tccctgtggt gatgataaat ggtgaaatta ggggactcga ctgttacaac ataagaaatg
      241 acagttcaat tgggataaac caattggtgg attatctggt atcgctaaac cataagaaga
      301 ttgcctttat tggtggatat acggacatcc aacccaccat gagaagatta aaaatcatta
      361 aagaatcttt aaatagatac ggtataggct ttaatggcga atggtttatt gaagacgatt
      421 tcagcgttca ggcagggaag gatagcatgg aaaagttgtt atatatgaag gacaagccca
      481 ccgccgttat atgcgtaaat gatatggtag cggtgggggc ccttcatacg gcaacaaaaa
      541 tgggggttaa ggtacctgac catatatcaa ttgccggctt tgacaatacc tatttgactg
      601 agtgcgtggt accggagata acaacggtgg atctgcgacc aaaaagactt gccaggaccg
      661 cagtagagac tatggttggg caccttgaag gggtaagggt agataaggag attacaatta
      721 aaacacaatt ggtattgcgg gattcctgct ctattgcaga ttagattggt ctgctctgta
      781 ccggattatt ataaaagatt cacgggaaaa aatgtattgc atttttttat aatagtgtat
      841 ataattgtta ttgtgctttt aaagccaatg atctctacca agggaaggag ggcggaacat
      901 gattgttaag ataagagata tcgttaagat aagagatgat gtaaagggta tccacggtct
      961 tttagccgtt gagcaaatac cggataatca tggcctgcct atgtctttgg ggattgggga
     1021 gaaactgtca gcttgatagt tttggtttta ttagctgggc catgggtaat aagaatatgc
     1081 ctgtggtttt tatttggata tattttacgc aatgataatt tcgtaaccgc aggcttaagg
     1141 tctgcggttt tttatggcag gattttccga tagaaaagga gtgaaaccat ggaggataaa
     1201 aaatggagtt atctttggaa gataacaaag ggcaaccgca tgctctatgt aggagcaatc
     1261 ataagtatgg ccctatcggc ggtgttgtcc ttatttcccc cgttggtgat aagtataact
     1321 gtggattcaa ttatagggga tgagccttta attgactctc ccgtggttgt atccatgatc
     1381 caaaggctag ggggaaaagg tgttctggcg cagaacctat ggatctgcgg attggtcctg
     1441 gttattctaa atatctttat cgggtatttt aattttacca ggggcaaatg gtccgcccaa
     1501 gcggcggaag gcatcgcaag gggcatcagg aataacctat atgatcacct ccagcatctg
     1561 ccctatgacg agcatgtgaa agcggagacg ggggatctca tccaacgttg cacctcggac
     1621 gttgaaaccg tacgcagatt cctggccatg caattggtgg agatgggtag agccatagtg
     1681 ttgctggtag cttctttgtt ctttatgttc tccttaaacg taaagatgac ctttgtatcc
     1741 atagccgttg tcccctttat atttcttttc tccttcattt tcttcagtaa gataaaggag
     1801 gccttcaagg aagccgacga ggccgagggt aggttatcca ccgttctaca ggaaaatcta
     1861 acgggcataa gggtagtcag ggcgtttggt aggcaggctt acgaggccga taaatttgat
     1921 gagaagaata ccgaatatac aaatctttgg taccgattga actcgctcat ggccttatac
     1981 tggtcatcat ctgacgtgct ggctatgggc cagatagggg cggtattggt gcttggggtt
     2041 tactgggcgg cacagggcga attaaccctg ggcaccctcc ttgcctttat gagctacgaa
     2101 tggatgctgc tatggccggt acgccagctg ggcaggatcc ttacggatat gggcaaaacc
     2161 caggtttcac tgggcaggat taaggagatt atagaaaagc ccctagagga tatgaatgaa
     2221 gatggattaa cccccccaat ggatggggat atagaattta agaatgtata cttcgaatac
     2281 gaaaagggtc gccccgtttt aaaggatgtt tcatttaggg tagaaggcgg gaagaccata
     2341 gccatcctag gatctacggg atctggcaaa tcctccttgg tgcatttgct tcaggggctc
     2401 tacgactatg acagaggctc tataaccatt ggaggggtag agcttaggga tatagagaag
     2461 agatatctaa gaaagcaggt gggtatcgta cttcaggagc ccttcctgtt ctccaagacc
     2521 ataaaggata acatcgcctt ggcaaaagtg ggtgcatcgg ataatgaaat ccataatgcg
     2581 gcgaatacag cagccgtcca tgacgtcata agagaattcg agaagggtta cgataccacc
     2641 gtaggcgaga ggggagtgac cctgtccggc ggccaaaaac aaagggtggc catggccagg
     2701 accctcatct tggatagttc tatccttata tttgatgatt ccttaagcgc agtggatact
     2761 gaaactgata atgccatcag gtcggccctt aatgaacgca gcagggatgt gacaacattt
     2821 ataatctccc ataggatcac cactatctcc caggcagatg tcatcttggt gatggaggac
     2881 ggacgaatag tgcaatcggg aattcacgac gaactgctgt ctcaggaggg gatgtatagg
     2941 cgtatctggt ctatacaaaa cgctctggaa gaagagctgg aaaagaattt agcgtagtat
     3001 acaccccatg aatgggaaaa gatggctggt tagtatcatt cttttttcat ggggatatgt
     3061 cggcaaaggg agggatatag ttgaacgaac taaaggagca agaatataat aaatccatcg
     3121 attttgggct atggaaggac ctgtttagat atataaagcc ctacagaaaa aggattattg
     3181 cccttatagt ggttatgatg gggtcagggg gaatcgatgt catatttccc ctgatgaaca
     3241 ggtacgccat agacaacttc attatgacgg gaaggctcga ggggctgtgg gtattcgcag
     3301 ggatctatgg gggattggta gccatccagg ctataaacgt ttactttttt atctacattg
     3361 cggggcgcat agaactgaac ctgagctacg atattagaaa ggtcggattt aaaaggcttc
     3421 aggaattgtc cttttcatat tatgacaaaa catccgtggg atggctcatg gcccgcatga
     3481 cctccgatgt agagcggttg ggagcaacca ttgcatggag tctggtggat ttaacctggg
     3541 gcggtaccgc catggtaggc gggttgatag caatgttata tatgaattgg aaactggctc
     3601 ttatttcatt ggcggtggta cccttcttgg ctgtaatcag catcttcttt caaaagagga
     3661 tcttagggtc gtatcgtatc gtaagaagga ccaactccag aataaccggt gcttttaatg
     3721 agggtattat gggggctaag accactaaga ccctggtacg ggagaaagaa aatctcaaag
     3781 aatttaaagg ccttacaggt gagatgtaca gctcgtcggt gagggcagca gtcttttcat
     3841 ccgtctattt tcccatagta ataaccatgg gaagcgttgg aaccgccctg gccctatggt
     3901 ttggtggcag gggagtcttg cttcaggcaa taggatttgg tacactatcc gcctttattt
     3961 cctatgtgat gcaattcttt ggtccgatca acgagctggc caggatcatc gccgaatttc
     4021 aaaatgctca ggcctctgcg gagagggtaa tggctatgat aaataccgag cccgacattg
     4081 tggacacggc ggaggtaata gagaaatatg gtacggaatt taagcccaga tacgaaaact
     4141 gggaagtaat cgacggtggc attacattca gtgacgtatc cttctcctat aaggaagggg
     4201 aagaggtcct agaggacttt agcctggatg tacaaagggg cgagtccatt gccttagttg
     4261 gcgaaactgg atccggaaag agtactatag tgaatcttat ctgccgcttc tatgaaccgg
     4321 tagagggtca gattcttata gacggtgtgg attatcggga gaggtctcag ctatggctcc
     4381 agtccaatat cggctacgtg ctccaaaccc ctcacctatt tagcgggacc gtcaatgaaa
     4441 atatccgata cggaaggctg gacgctaccg atgaggaggt agaggaagca gccagactag
     4501 tcaacgccca caatttcata atgaagatgg aggacggtta tgattcggaa gtaggggagg
     4561 gcggcaatag gctctccacc ggcgaaaagc aactgatttc ctttgccagg gctatcttgg
     4621 cggatcccag gattttggta ctggatgagg ccacctcatc catagacacc gaaaccgagc
     4681 agatgataca atacgccatc aacaagctgt tggagggcag gaccagtttt atcattgccc
     4741 accggctttc taccatacga tctgcggata ggatattggt gataaaggac gggcgtatga
     4801 tagagcaggg taatcacaga gaattgatgg gactgggagg atactattac aggctctaca
     4861 ccaatcagtt tagggaagag caagagttga gcgccataaa cgcatagagg attaaagccc
     4921 gggggttagc ttccccgggt tttgttatgt attgattttt tagcagcctt acattagaat
     4981 ataattgttg ccaaggcaat ggagtaagta catcgtagtt tgcaggagga tgagattttg
     5041 tgagagagct ttgggaatta ttcagcgttt tttttcggat aggcgcattt acctttggag
     5101 gcgggtatgc catgcttcca attatccaaa gggaggtagt agagaagagg gaatgggcga
     5161 cggacgagga agtgatagat tactatgcca tcggccagtc tacccccggt ataattgccg
     5221 taaatacggc aatctttatt ggatataaga acaaggggat tattgggggg atttttgcga
     5281 ctgccgggat ggtattccct tccctagtga tcatcatggc catagcaagt tcctttatgc
     5341 attttcagga gaaccttata gtccagcatg cctttgcagg tgtaagggtc gcggtggttg
     5401 ccctggtagt aaacgcagtg gttaggatgt ggaagacttc ggtaaaggat gtcgtcggaa
     5461 taatactatt taccgtagct tttttggtca ttgcctttac ccggatttcc ccgatcatcc
     5521 tgatattcgc gtcggcagtt acgggaatca tactcacatc taggaaggcg gtggacaagt
     5581 gatctatcta taccttttct tcgaattctt taaggtgggt ctctttgccg taggcggagg
     5641 cctggcaacc ataccctttc tccaggagct aatacaaaag tacgggtggg tgacacccca
     5701 ggaattgatc aacatgatag ccatatcaga atcaaccccg ggtcctatcg gtgttaacat
     5761 ggccacctat gtgggttttg agaccgccgg tataggcggc gctatattag ccacagtcgg
     5821 cttggttacc ccatctgtta taataattgt aataatagcc cattatttta tgcggttcgc
     5881 cgaggaaccc ttggtgaagg cgggatttta cgggcttagg cctgcagtta ccggactcat
     5941 cgccgcagcc ggctttgagg tagccagggt ttcgctattc aacctccaaa gatacttaga
     6001 gacaaaaaaa gtcttggata tattggatcc aaaggcgata gtgctctttg gtatactgat
     6061 gtatgttacc aacaagtaca aaaaacaccc gataatatat ctcgtcgccg ccggggtggt
     6121 gggggtaata tttaagtttg ggtgaagtta gccttgttaa tcggcgcaag agaagacttc
     6181 atgtgcgagt taaatatacc ggtaatgaca ctagaacgcc cagttacatc gtaaaagtaa
     6241 tggggagatg gtttattccg gcattttcca aactacatcg ggaagctgct ttacgggccc
     6301 catctttggg ggattattag cagcccttcc ctcagtggtc tttctgtcgg gccgttggaa
     6361 tctgcctcca caaatccatc cctcctagca acagttctat ggaccgggct tctaacttct
     6421 aatttccaat ttctgatttc tgggagccca atcaatgtag tcatccgctt atcaatagct
     6481 cattactgtt atcacactgc aataaaatga ttttagatgg ggaaggtgaa atttatgggt
     6541 agagtttgtg agtggaaaaa ggttagggac gatgaggtaa atccattgat ttgggaacaa
     6601 atagaccccg tcctaatcga agaatatcca tgggacgaga acggatacaa gccaagggtc
     6661 gaggtgaggt ttttctatac cgaatcccgt ctgcatcttc aatttacgtc ctatgaaagt
     6721 gaaatcaggg cggtctacag gaatatgaac gagccggtgt ataccgacag ctgtgtggaa
     6781 ttcttcttta atccggaccc ccatagggat gacaggtact ttaattttga aattaacgct
     6841 ataggaacat tgctgttgga actgggcaat gacagaaata acaggctgat gattcgagat
     6901 accccccagg acatattctg cataaaatcc tctgtcagcc ccgacaacgc agatgaatat
     6961 gaaggtgact cctggatttt ggagctttcc ataccctact cctttattga aagatattat
     7021 ggcagccagg atttttcacc gggtaagagg atgaggggta acttccaaaa atgtggagac
     7081 aagaccaaat ttccccacca tggaagctgg aacaggatag atacactaga ggcggacttt
     7141 cataggcctg aatacttcgg agacctgata cttaaatagc cggagttccc tgcctatgca
     7201 ccgcattcag cttgcccgcc aatttttggg caggtcgtca taggcattag agacggcggt
     7261 gatgcccgcg attaggaact gggggtcaac tgattttttt ggtgtatcta ttggcgtaac
     7321 agctggcagc gtagctttcg ggctttatct tgggctcaaa accggtaccg ttagccatgg
     7381 ccaccatctc gctgattaat tctctggtgg ggcccacagt acctacatca agatgaaatt
     7441 caaaatgaat atcacctggg cggcccatgg tggttaggat acaatccacc aacttatcca
     7501 tcttttcgtc ggtaaaccaa gcggccagtt ccaaggtctt actggtttca tagtagatct
     7561 tttccctaag actttttata ggtctgggaa aaaattgcgt tgtaaaaaaa cccgtcgcac
     7621 cgcggcctac cttatggaga tgaatggctg tcacaaatac cgtttcatgt gccccaacct
     7681 gagaatccgt tccgatggat agacgatatt cgttgtgtgg ggcttgcctt aaaaactgta
     7741 cgagcatttc atacacttga tccagggtca aatttttatg cattgaattt ctaaacaatg
     7801 aatccctgat gataatcact tccccttggg ctttgaaatt atatttggct gtccggcatc
     7861 tacatgttcc acgataaaga tcctgcaccc tggtgcagac atcgtagaac agcttcccta
     7921 ttatataggt tacgaaaaca cgctgtttta gtgcaaaacc aaatggctta gctgatgact
     7981 caactgcctt aagatgataa tctttttcgt aacatgccca ttatatatta tctactgata
     8041 attcgcaatt tttcatgttc tattgggcga ataatccgaa ccaggaaaat aatattccca
     8101 tcgcttaagt tccaagg
//
LOCUS       DASRNX010000019         6891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_55841,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000019 DASRNX010000000
VERSION     DASRNX010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55841"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..472)
                     /locus_tag="VFD89_00710"
     CDS             complement(83..472)
                     /locus_tag="VFD89_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HZJ56745.1"
                     /translation="MERSLNVLICVTRQISCERLIRTGYDVSRKSGGRPHVIHVAPIG
                     ENFLGNPREGEALDYLFDISKQVGAEMTVLRSNEVIEVLTDFTQKHKIGTMVLGESAN
                     PQCGREGEIIKQLKKKLRNVEFIVVPA"
     gene            complement(727..2094)
                     /gene="radA"
                     /locus_tag="VFD89_00715"
     CDS             complement(727..2094)
                     /gene="radA"
                     /locus_tag="VFD89_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HZJ56746.1"
                     /translation="MAKLKKVFVCQDCGYESIRWMGKCPSCNEWNTMVEEIEQPVQKR
                     TLSGAQRATGVSRPVSLGEVSLRQENRESTGLEELDRVLGGGVVNGSLILIGGDPGIG
                     KSTLILQICESLGKGDDTILYASGEESARQIKMRANRLGVSSEGLRLVAETNIDVILA
                     HVEAIKPRFLMVDSIQTMFNPALSSAPGSVSQVREVTATLMGLAKKSDMTVFVVGHVT
                     KEGAIAGPRVLEHMVDTVLYFEGDRHHTYRILRGVKNRFGSTNEIGIFEMGEKGLIEI
                     MNPSEHLLSERTHGMSGSAVLCSIEGTRPMLVEIQALISTTAFGMPRRMSTGVDYNRV
                     VLLLAVLEKKVGMRLYDQDAYVNVAGGIKIDEPAADLAVICSIASSFRNVSIDHHLCI
                     MGEVGLTGEVRGISQAEKRILECRKLGFKTCLIPKANLKGMDRIKDINIIGVDTVSQA
                     LGIVL"
     gene            complement(2298..4733)
                     /locus_tag="VFD89_00720"
     CDS             complement(2298..4733)
                     /locus_tag="VFD89_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HZJ56747.1"
                     /translation="MAFFGRFTERAQKALLYAQEEARSIGHNYVGTEHLLLGLLREEG
                     GAAAKVLKDLGMEIEKMREYIVNLVGKGNFNFSEGFGYTPRTKRVMELSFYEARNLSH
                     NYVGTEHLLLALIREGEGVAARIMKDADIDLEKIRGLVMKILQEEGTETHQGGSPGGK
                     TDTPNLDQFGTDLTVMAREGKLDPVIGRENEIERVVQILSRRTKNNPVLIGEPGVGKT
                     AVAEGLAQRISEGNIPELLKGKRVVTLDLSGMVAGAKYRGEFEERLKSVMSEIKESKD
                     IILFIDEMHTIIGAGAAEGAIDASNILKPALSRGEIQAIGATTLDEYQKHVEKDAALE
                     RRFLPVMVGEPSVEETVKILYGLRDRYEAHHKVRISDEAMESAATLSDRYITDRFLPD
                     KAIDLIDEAASRLRLQTYTTPPDLKNLENDLEDIRKEKEEAVANQNFEKAAQLRDREQ
                     KVKGEIEAEKNKWKTSHATGTGIVGDHEIAEIVSSWTGVPVVKLTEEESDRLLNMEEV
                     LHKRVIGQEEAVHAVSRAVRRARVGLKDPRRPVGSFIFLGPTGVGKTELCKALGEALF
                     GDEDAMIRIDMSEYMERHTVSRLVGSPPGYVGYEEGGQLTEKVRRKPYSVILLDEIEK
                     AHPDVFNILLQILEDGRLTDAKGRLVDFRNAVIVMTSNVGAHTIRKQRTLGFSPVDNA
                     VDSEYEKMKENILEELKKTFRPEFINRIDEVIVFHSLDDEHLRRIVPLMLENAIERLK
                     ARDIYLEISDAAKDHMVKEGFDPVYGARPLRRAIQRMVEDNLSEEILAGRIKVGDQVL
                     IDVENDKLKFNKK"
     gene            complement(4755..5780)
                     /locus_tag="VFD89_00725"
     CDS             complement(4755..5780)
                     /locus_tag="VFD89_00725"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HZJ56748.1"
                     /translation="MNWINKTGPLGDVVLSSRIRLARNLSDTPFPSVADKSMTDSVIH
                     RVHRSVSGQDSPMDSEYQFLAMKDLAPMDREILVEKHLASPDLLENPDRSAILINREE
                     ICSIMINEEDHIRMQCIFPGYQLKKALEYLGGIDDALEGQLPYAFDADLGYLTCCPTN
                     LGTGMRASVMMHLPLLAATGQMKAIVQTLPKIGIAVRGLYGEGSEAVGDIYQISNQIT
                     LGASEEEIVMSLEVVTKQIVNKEKEARKRVMQRGKVRFEDGIWRAYGTMTHARIMDLN
                     EFMGLLSKVRLGVEMGIIPNIDIKAINELMVMGQPAHLKKYQDGDTGIKDLDEVRARV
                     VAKKLKQ"
     gene            complement(5785..6297)
                     /locus_tag="VFD89_00730"
     CDS             complement(5785..6297)
                     /locus_tag="VFD89_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HZJ56749.1"
                     /translation="MLCQECKKNPATVHVTKIINNVKTEFHLCQRCAGERDDLNWFSP
                     FSINDLLTGFLEPIKSTMAAEAAPAIRCDKCGMDYRQFKKTGRFGCDNCYSVFKGEIH
                     PIIKRIQGGASRHTGKSPARQDSRLKTNKEIEDLKRQLQGAINIEAFEEAAKLRDRIK
                     DLEKKQEQGG"
     gene            complement(6322..6786)
                     /locus_tag="VFD89_00735"
     CDS             complement(6322..6786)
                     /locus_tag="VFD89_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HZJ56750.1"
                     /translation="MARLSDIIEDFIKSLIKQSEGELEFQRNELAEYFECAPSQINYV
                     LATRFSLDRGYHIESRRGGGGFVRVIQLDIDEDDYLLYLVNQRIGTTISQQASKDIVT
                     NIMERGIINEREAAIIEAGLMDKVIGIPTNLRGVIRANILKAMLVAVLKTQG"
BASE COUNT         1428 a         1993 c         1467 g         2003 t
ORIGIN      
        1 gcataagaag aggcaattgt caataaactg tttattttag cacgatggta ttcatttgtc
       61 aacacgaatt ttcgaaatct ttttatgccg ggaccacgat aaactccaca tttctaagct
      121 ttttcttcag ttgttttata atctcgcctt ccctgccgca ctgcggattc gctgattcgc
      181 ccagaaccat tgtcccgatc ttgtgctttt gggtaaagtc ggtcaaaacc tctattacct
      241 cgttggaccg aagtaccgtc atctcggccc ccacctgttt tgaaatatca aaaaggtaat
      301 ctaaggcctc gccttctctg ggatttccta aaaaattctc ccctatggga gctacgtgaa
      361 ttacatgggg cctgcccccg ctttttctgg atacgtcata tcccgtccta atgagccttt
      421 cgcaggatat ttggcgggtt acacaaatca atacattaag tgatctttcc atctgtaccc
      481 cccttgcctt attgtgtctt ggataatctc tgaacctccc atgactaaag tcacgggttc
      541 tagagttcaa atgaactcat ttacgcctct gtagcctaag ctatcttgat gcgtacaggc
      601 gtgtccagtt cgcctctacc gtatagccct gttaagggca caacgctact tttatgggat
      661 tctccaattc atctcacgac ttaagtctcg agtgttcatg aggagcctat aaaattatta
      721 tgatgactac aaaacaatgc caagagcctg ggacacggtg tccactccga ttatgtttat
      781 atcctttatc ctatccatcc cctttaggtt ggcctttggg ataaggcagg tcttaaaacc
      841 cagctttctg cactctaata tccgcttctc cgcctggctg atacccctta cctccccggt
      901 aagcccaacc tcacccatta tacataggtg gtgatcgatg gaaacgtttc tgaagctcga
      961 tgctatggag cagattaccg ccaaatccgc cgccggttcg tcaatcttga tgcccccggc
     1021 cacgtttacg taggcgtctt ggtcgtatag gcgcatgcct accttttttt ccaggaccgc
     1081 cagcaaaagc accaccctgt tgtagtccac cccggtggac attcgcctgg gcataccgaa
     1141 ggccgtagtg cttataaggg cctggatctc caccagcatg ggccttgtgc cctctatgct
     1201 gcaaaggacc gcagacccgg acatgccgtg ggtccgctcc gaaagaaggt gctctgaagg
     1261 gttcattatc tctataaggc ccttctcccc catttcgaat atccctatct cgttggtgga
     1321 cccaaaccgg ttttttacgc cccttaggat tctgtatgta tggtggcgat ccccctcaaa
     1381 atacaggacc gtatccacca tatgctctaa aaccctaggc ccggcgatgg cgccttcctt
     1441 ggtaacatga cccaccacaa agacggtcat atcgctcttt ttagcaagcc ccatcaaggt
     1501 ggccgttacc tccctgacct ggcttacgct gcccggcgcc gaacttaggg ccggattaaa
     1561 catggtctgg atggaatcta ccataagaaa acggggcttt atggcctcta catgggccaa
     1621 gatgacgtct atgttggtct ccgccaccag gcgaaggccc tctgacgata ccccaagcct
     1681 gttggcccgc atctttatct ggcgggcgga ttcttcgccg gaggcataca gtatcgtatc
     1741 gtcgcccttc cctaaggatt cacatatctg aagtatcagg gtggactttc cgataccggg
     1801 atctccccct attaggatta gggatccgtt taccactccc ccgcctagga ccctgtcaag
     1861 ctcctctagg ccggtactct cccggttctc ctgccgcagg gaaacctccc ctagactgac
     1921 gggacggcta acgccggtgg ccctttgggc cccgcttaga gtcctctttt ggacgggctg
     1981 ctctatctcc tccaccatgg tgttccattc attacaggag gggcatttgc ccatccatct
     2041 gatggattcg tacccgcagt cctggcatac aaatactttc tttaacttgg ccatgtgcac
     2101 tgattgctcc catttctcat ttgttttatt cggcactgct attagattat accataagtg
     2161 cgggcaaaaa tatatgaact aatgcaaaca cctgggcgcc ggggcaccgc catgcgggag
     2221 cgcaaggtgc gctgcgccag gcgggtgcgc ttaagcgcag tgcacaaggg gacggtcccc
     2281 ttgtgttcct tgtgttctta ctttttgtta aattttagtt tatcgttttc tacatcgatt
     2341 agaacctgat cccccacctt tatcctgccg gccaggatct cctctgacag gttatcctcc
     2401 accatgcgct ggatggccct acgtaggggt cttgctccgt atacggggtc aaagccctct
     2461 ttaaccatat ggtccttggc cgcatcgctt atctcaaggt agatatccct agccttcagc
     2521 ctctctatgg cattttccag catcaggggt actatgcgcc ttagatgctc gtcgtctaag
     2581 gaatggaaca cgattacctc gtcgatacga tttataaact cgggtctgaa ggtcttctta
     2641 agctcttcta agatgttctc cttcatcttt tcgtattcgc tgtccacggc attgtcgacg
     2701 ggtgaaaagc ctagggtcct ttgcttgcga atggtatggg cgcctacgtt tgatgtcatg
     2761 actattacgg cgtttctaaa gtccaccagc ctgcccttgg catccgtcag ccttccgtcc
     2821 tccaatatct gaaggaggat gttgaacaca tcgggatggg ccttttctat ctcatccaat
     2881 aatattacgg aatagggctt tcttctgacc ttttccgtca attggccgcc ttcttcgtag
     2941 cctacatatc ccggaggcga acctaccagc ctggatacgg tgtgcctttc catatactcg
     3001 gacatatcga tgcggatcat ggcgtcctcg tcgccaaaca gggcttcacc tagagcctta
     3061 cacaactccg tcttacctac cccggtgggg cctaagaata taaaggagcc tacgggcctt
     3121 ctgggatcct tcagcccgac ccttgccctt cttacagccc gggataccgc atggacggct
     3181 tcctcctggc ctattaccct cttatggagt acctcctcca tgttaaggag cctgtcggat
     3241 tcctcctccg tgagcttcac cacggggact ccggtccaac tggatacgat ctcggctatc
     3301 tcatggtcgc ctacaatccc ggtcccggta gcgtggctgg tcttccactt attcttttcg
     3361 gcctctatct cgccctttac cttttgctct ctatctctaa gctgggccgc cttctcaaag
     3421 ttctggttgg ccacggcctc ttccttttcc tttcggatgt cctcaagatc attttccagg
     3481 ttcttaaggt ccggcggagt ggtataggtc tgaagcctca gcctggacgc tgcctcgtct
     3541 atcaaatcta tggccttgtc cggcaggaac cggtccgtta tatatcggtc cgaaagggta
     3601 gccgctgatt ccatagcctc gtcgcttatc ctcaccttat ggtgggcttc atagcggtcc
     3661 ctcaggccgt agaggatctt tacggtctcc tctacgctgg gctcccctac cattacggga
     3721 aggaaacgcc tctcaagggc cgcatccttt tccacatgct tttggtattc gtccaaggta
     3781 gtggccccga tggcctggat ctctcccctg gatagggccg gctttaggat gttggatgca
     3841 tctattgccc cttccgctgc cccggcgcct ataatggtat gcatttcatc tataaacagt
     3901 attatgtcct tggattcttt tatctcgctc attacggatt ttagccgctc ttcaaattct
     3961 cccctgtact tcgccccggc taccatcccg gaaagatcca gggtcactac ccttttgccc
     4021 tttaaaagct ccgggatatt gccctctgag atcctttggg ctaacccctc ggccacggcg
     4081 gtcttaccca cgccgggctc gcctattaag acgggattgt tcttggtcct acggcttagg
     4141 atctgtacga ccctttctat ctcattttct ctgcctatga cgggatccag cttgccttcc
     4201 cttgccatca ccgtaagatc cgtcccgaat tggtccaggt tcggggtatc ggtcttaccg
     4261 ccaggggaac ctccctgatg ggtctctgtg ccctcctctt ggaggatctt catgacgagt
     4321 ccccttatct tttcaaggtc tatatcggca tccttcatta tcctggcggc gacaccttcg
     4381 ccctctctta taagggccag cagaaggtgt tctgtgccga cgtagttatg gcttaaattt
     4441 ctcgcctcgt agaagcttag ctccatgacc ctcttggtcc taggggtata gccaaagccc
     4501 tcgctgaaat taaagttccc cttacccacc agatttacga tatattctct catcttttca
     4561 atttccattc ccaaatcctt cagcaccttt gcggctgctc cgccctcttc ccgaaggagg
     4621 ccgaggagga gatgctcggt tccgacgtag ttatgcccta tgctccgagc ctcttcttgg
     4681 gcatataaaa gtgctttttg tgctctctct gtaaatcttc caaaaaacgc cattcgcgta
     4741 cctccattct ctatctattg cttcagtttc tttgccacaa ctctggccct tacctcgtcc
     4801 aaatccttga tgcccgtatc gccgtcctga tattttttaa ggtgggccgg ttgccccatg
     4861 accatcaact cgttgatggc ctttatatct atgttaggta ttatacccat ctcaactccc
     4921 aatctaacct tcgacaaaag tcccataaac tcgtttaaat ccattatcct ggcgtgggtc
     4981 atggtgccgt aggctctcca tattccgtct tcaaagcgca ccttgcccct ttgcataacc
     5041 ctctttcgag cctctttttc cttattcacg atctgctttg tgaccacttc caggctcatg
     5101 acgatctcct cttccgatgc gcccagggtt atctggttgg atatctgata gatatccccc
     5161 acggcctcgc tgccttcgcc gtatagtccc ctcaccgcta tcccgatctt aggtagggtc
     5221 tgcactatgg ccttcatctg gcccgtggcg gccaggagcg gaaggtgcat catcaccgat
     5281 gccctcattc ccgtacccag gtttgtggga caacaggtaa ggtatcctaa atcagcgtca
     5341 aaggcgtagg gcaattgccc ctccagggca tcgtcaatac cgcccaaata ttccagagcc
     5401 ttctttagct gatagcctgg gaaaatgcac tgcatcctta tatgatcctc ttcgtttatc
     5461 atgatgctgc atatctcttc cctgtttatt aggattgcgg acctgtcggg attctctaaa
     5521 aggtccgggc ttgccagatg cttttccacc aggatttccc tgtccatagg cgccagatcc
     5581 ttcatggcca agaactggta ctcagaatcc atgggcgaat cctgacccga aacacttcgg
     5641 tgtaccctgt ggataacgga atccgtcatg gatttatccg ccacagaggg aaatggggtg
     5701 tcggataggt ttctggccag ccggatgcgg ctgctgagaa ccacgtcccc caatggcccc
     5761 gtcttgttaa tccagttcat agacttatcc cccctgctcc tgtttcttct caaggtcctt
     5821 gatgcgatcc cttaactttg ccgcctcttc gaaggcctca atatttatgg cgccctgaag
     5881 ctgtcgcttt agatcctcta tctccttatt ggttttaagc cttgaatcct gccttgcggg
     5941 actcttcccc gtatgacggg aggctccgcc ttgtatcctc tttattatgg ggtgtatttc
     6001 acctttaaat acgctgtagc agttatcaca cccaaaccgc ccggtctttt taaactgtct
     6061 atagtccatc ccacacttat cacaccttat ggcgggggca gcttcggctg ccatggtgga
     6121 ttttatgggc tctaaaaatc cggtcaaaag atcatttatg gaaaacggcg aaaaccagtt
     6181 aaggtcatcc ctttcaccgg cacatctttg acatagatga aattcggtct tcacgttgtt
     6241 gataatcttg gtcacatgaa ccgtcgcagg attcttcttg cattcctgac ataacatctt
     6301 aattacctcc cctcaaagac tttatccctg agtcttgaga actgccacca acatagcttt
     6361 taatatgttt gcccttatta cccctcttaa attggtagga ataccgatga ccttgtccat
     6421 gagcccggcc tctatgatag cagcttccct ctcattaatt atacccctct ccattatatt
     6481 tgtaacaata tcctttgatg cctgttggga tatagtggta ccgattcgct gatttacgag
     6541 gtataatagg taatcgtctt cgtctatatc caattgaatg acgcggacaa aaccgccgcc
     6601 gcctcttctg ctctctatgt gatatcccct gtctagggaa aatctggtgg ccaacacgta
     6661 attgatctga gagggcgcac attcaaagta ttcagccagt tcattcctct gaaattccag
     6721 ctcaccttcg ctctgtttaa tcagggattt tataaaatct tcgattatat cgctgagtct
     6781 ggccattatt atcactccct ttcatcttga ccatctttga ccttcatatt atattatacc
     6841 ttatccccga aaaaacataa gtcgggaatt aattcattac accccatact c
//
LOCUS       DASRNX010000020         6001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_587281,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000020 DASRNX010000000
VERSION     DASRNX010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587281"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /locus_tag="VFD89_00740"
     CDS             complement(<1..1889)
                     /locus_tag="VFD89_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZJ56751.1"
                     /translation="MSIIILAASTSRAHRHLIIRTLNEYQILLASSSYEVSHYLSMQV
                     NIDLIIVDLDAHGEEGFRILEGLNSKGHIGKTNIIVFPGSDRIRQEIGTLKYDNLDYI
                     KEPIGSESLKAGVKAHLKSLQSCKRTEPRKQIQNIEKALAESERSKNVFLSHLPGMAY
                     RCNYDPQWTMQFVSEGCLDLTGHPSESLLYNRGISYNSLIAYEYRQQLWEEWERILAE
                     KRLFKGEYEIITADGKRKWVLERGQGVYNRQGEVEALEGIILDISDRKEMENDLRYNY
                     EHDTWTGLYNRRYLEGLLRHDFDTWAGRRAVIAINLSTVQSLTLTYGFQYSQELIKKV
                     AYALRPHCNGKRSLFRVQENQFIFYVKAYKDKGELLEFCSAIADTLGEILTIERIGGG
                     IGVLEVGEENKSDIEQLLKNLLIISEEAINVPDREIGIRFFDAEMEIQVNREEIIKRE
                     LARIAIHRRGDGFFLQYQPILDLKTNRICSFEALARLKISSIGPVSPMEFIPIAEKTK
                     LIIPLGEIIIYQALSFQKMLVEHGYNDISVSINISAIQLFRDDFSDFLCSCMREVGVN
                     PKNIILEITESVFASNYENINDIIEKLKNCGTKIALDDFGTGYSSLAQSRELDINYLK
                     IDKYF"
     gene            2552..3493
                     /locus_tag="VFD89_00745"
     CDS             2552..3493
                     /locus_tag="VFD89_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZJ56752.1"
                     /translation="MLFRKLGSSELNCSVVALGTWGMGGDFWGKADDDASIDTIRAGL
                     DAGINTIDTAPVYGDGHSEEIVGKAIKGRKREDIIIATKCGIDIVGKTGRNSSPELLF
                     KEIDASLKRLGTDYVDLYQVHWPDLDTPLEETFTALKQIAESGKVRYIGVSNYSPAQM
                     DEASKYCEIVSLQPPYSLLQREIEDEILPYCIEKGIGVLSYGSIGAGALTGKFKTRPI
                     FGDDDSRDRFYDFFSEETWPKTDALVDVLRDIAESRGKPVVHVAINWVLKQEGITVAL
                     VGARTPEQAIMNAQAGEWSLSDEENKIIGDAYKKIFG"
     gene            complement(3686..4735)
                     /gene="ispG"
                     /locus_tag="VFD89_00750"
                     /gene_synonym="gcpE"
     CDS             complement(3686..4735)
                     /gene="ispG"
                     /locus_tag="VFD89_00750"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZJ56753.1"
                     /translation="MTRAKTRKIWVGEVALGGDSPISIQSMTNTDTRDVRSTVSQIRD
                     LEDAGCHIIRCAVPDMEAAEALRDIVNQINIPLVADIHFDYRLALSSIENGAQKLRIN
                     PGNIGSREKVKAVVEAAKDKGIPMRIGVNSGSLQRDILKKYGQVCPEALVESALRHVE
                     ILDELDFGDVAISVKSSDVMETIESYRLLSERTDYPLHIGVTESGTVKRGTVKSSVGI
                     GALLAMGIGDTLRVSLTGDPVEEIAVGREILKSLGLLEGGIEFISCPTCGRTQIDLIK
                     IANEVEERLGNLDKNIKVAVMGCAVNGPGEARRADIGIAGGRGEGLIFKKGEIIKKVK
                     QENLVEELIKEIEKL"
     gene            complement(4728..5603)
                     /gene="ispH"
                     /locus_tag="VFD89_00755"
     CDS             complement(4728..5603)
                     /gene="ispH"
                     /locus_tag="VFD89_00755"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZJ56754.1"
                     /translation="MQIIVAENAGFCFGVTRAVDITIKQREGGNPGRTYTLGPLIHNN
                     DVVDSLRKEGIYPLSFDEIDKLVEDDTIVIRSHGVTPETIDEIRNTGAKIIDATCPYV
                     TSIQRKARKYHDKGYQIIIVGNKDHPEVIGINGWCYNSAVITRDGSDLDKLPQRVCIV
                     SQTTERLSNYEKALKVVSEKCEDVLSFNTICSATKERQASAYEVSKDVGLMIVIGGRN
                     SSNSKKLYEICSKNCGNTIFIENSSELSAEAVKKMLSEGVEKVGVTAGASTPDWVINE
                     VMEKLEELGREIDND"
     gene            complement(5594..>6001)
                     /locus_tag="VFD89_00760"
     CDS             complement(5594..>6001)
                     /locus_tag="VFD89_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HZJ56755.1"
                     /translation="SELIKNGFGEEEGLNCAEKILYGANVAYKLGLDKEALKLSAAFG
                     GGMAIEDKCGALTGALMVLGKLFVKEKAHESDKIGALTRELFKEYGMEMGYIDCTPLK
                     KEYRSEEEKCNYVILKAAQILDKIVTRETHLCK"
BASE COUNT         1558 a         1461 c         1175 g         1807 t
ORIGIN      
        1 atgaagtatt tatcgatctt tagataatta atgtccaatt cccggctttg agcaagggaa
       61 gagtatccgg tcccaaagtc gtccaaggca attttcgtcc cacaattttt caatttctca
      121 atgatatcat tgatgttctc atagttagag gcaaaaacgg attcagtgat ctccaaaatt
      181 atattttttg ggttaacgcc cacttcgcgc atacaactgc ataaaaaatc agaaaagtcg
      241 tccctaaata gctgaattgc agagatgtta atggatacgc taatatcatt gtatccatgt
      301 tccacaagca tcttttgaaa gctaagggcc tgatatatga tgatttcacc caatgggatt
      361 atgagctttg tcttctcggc aatcggtata aactccattg gagaaacagg gcctatactg
      421 ctgattttta gcctggccaa ggcctcaaag ctgcatatcc tgttagtttt aagatccaat
      481 ataggctggt attgcagaaa aaagccatcg cctctcctat ggatagcaat ccgggcgagt
      541 tcgcgcttta tgatctcttc ccggtttacc tggatttcca tctccgcatc aaaaaatcgg
      601 atgcctatct ctctatccgg gacatttatt gcctcctcgg atatgattaa aaggttcttt
      661 aaaagttgtt ctatgtcgga tttattttcc tcgcctactt ccaagacccc gataccccca
      721 ccaatccttt caatggtgag gatttccccc agggtatctg ctatagcgct acaaaattcc
      781 agtaattccc ccttatcctt gtatgcctta acataaaaga taaattgatt ttcctgtact
      841 ctaaataatg aacgctttcc attacaatgg ggcctcaggg cataagccac tttttttatc
      901 agttcctgac tatattgaaa tccgtaggtc agggttagag actgtacggt acttaagttt
      961 attgctatga cagccctcct tcctgcccat gtatcaaaat catgcctcag aaggccttct
     1021 aaatatctac ggttatatag ccccgtccat gtatcatgtt cgtaattgta tctaaggtcg
     1081 ttctccatct cttttcggtc tgaaatgtca aggattattc cctcaagggc ttccacctcg
     1141 ccttgtcggt tatagacgcc ctggcccctt tccagaaccc attttcgctt cccgtcagcc
     1201 gtgataattt catattcgcc tttaaatagt ctcttctctg cgagaatgcg ttcccattcc
     1261 tcccatagct gttgacggta ctcatatgct attaaactat tgtaggatat acccctgtta
     1321 tacaataggc tttccgaggg atgtccggtt agatcaaggc agccttcgga aacgaattgc
     1381 atcgtccatt ggggatcata gttgcaccta tatgccatac cgggaagatg ggataaaaaa
     1441 acgttcttac tgcgctcgct ctcggctagg gctttttcga tattctgaat ttgttttctg
     1501 ggttctgtac gtttgcagct ttggagtgac ttaagatggg ctttgacacc ggcctttaat
     1561 gactcgcttc cgatgggttc tttgatataa tccaaattat catattttaa cgtcccaatt
     1621 tcctgtctaa tcctgtcgga gcctggaaag acgatgatat ttgttttccc aatgtgtccc
     1681 tttgaattta gtccctcaag aatccgaaaa ccttcctcgc catgtgcatc taaatcgacg
     1741 ataattaaat cgatgttcac ctgcatactc aaataatgag atacctcata gctgctggat
     1801 gccaatagaa tttgatattc atttaaagtt cttataatta agtgacgatg tgctcttgaa
     1861 gtgctggccg ctaaaatgat aatagacata aattcaccct cttgtttaaa ttgggcctgt
     1921 tgtttatttt taacccttta gctttacaat accttggcat aatttgttta aaaatggcca
     1981 tccctacaat ctcttttatt catacctcat atccctatcc tttaatcatg atttacgccc
     2041 atagccggat atatccccat tcttataaat ggcaagccct ctagcaagcc ccttttaaat
     2101 agataaggct atgggtatag aatctttgtg tcgtgaaaat cgatgcgttg taatggcatg
     2161 gtaggcttcc tttttcgaaa ttgttgcaga tcatggcata aatgtatata atatagatag
     2221 aaaacaaaaa taggaacgaa tgaagggtgg gaaaggatgc tttaattccg ggattaaact
     2281 tttccgatgc tcgaaacgcc atggatgcaa accgtgggat agggtaataa tctaaaggta
     2341 gtaagggagt ttaaacatgt atcattcagg tgtttctgct atcagatgat agtttgagtt
     2401 cttaaaacct cagctataga taaacaactc caactacaaa atttatcacc atccgataaa
     2461 tttatttacc gtattgttta tctacaacca gttatttttt tggtaatacc ttatggtaaa
     2521 tcaattaata aatttaacag gaggaaagat tatgcttttt agaaaattag gttccagtga
     2581 attaaattgc tccgtcgttg cactaggtac atggggtatg ggaggtgact tttggggcaa
     2641 ggcagacgac gatgccagca tagacaccat ccgcgcaggt ctggatgccg gaattaatac
     2701 catagacaca gccccggttt acggtgacgg acactcggaa gaaattgtcg gtaaggctat
     2761 caagggcaga aaacgcgaag atataatcat cgcaaccaaa tgcggtattg atatagtagg
     2821 gaaaaccgga agaaattcct ccccggaact gttgtttaaa gagatagatg cttctctcaa
     2881 gcgtttggga acggattatg tggatctata tcaggtgcac tggccggatc tggatacgcc
     2941 ccttgaggaa acgtttaccg ctttaaagca aatagcggaa tcaggaaagg ttagatacat
     3001 aggcgtatca aattattcac cggcacagat ggatgaggca tctaagtact gtgaaattgt
     3061 cagcctacaa cccccttatt cacttctcca aagggaaatc gaagatgaaa tactgcccta
     3121 ttgtatcgaa aaaggcatag gggtcctttc ctatggttcc atcggtgcag gagcattgac
     3181 aggtaagttc aagacccgtc ccatttttgg cgatgatgac agtcgggaca gattctacga
     3241 tttcttcagc gaagagacct ggcccaagac ggatgcgctg gttgatgtac tgagggatat
     3301 cgcagaatcc cgcggtaaac ccgttgtcca tgtagctata aactgggtgc taaagcaaga
     3361 gggtattaca gtagcgttgg tcggagcgag aacaccggag caggctataa tgaatgccca
     3421 ggccggagaa tggtccctgt cagacgaaga aaacaagata ataggagacg cgtataaaaa
     3481 gatattcggg taattacata cgccaaggcc gccttcgggc tttatgcata atgtattagc
     3541 caaacgataa aatagcggtc gaacgataaa atagtagccg tcaattaggg gaaaaccccc
     3601 gttgacggct acttttaata agtatcagtc gtcttatccc ttttctgact ttaacccgct
     3661 tcttgttccc ccaacaatcg tgttcttaaa gcttttctat ctcctttata agctcctcca
     3721 ccagattctc ctgctttacc tttttgataa tctcaccctt tttaaatatt aaaccttctc
     3781 cccttccccc tgcaattccg atatcggccc tccttgcctc tccgggtccg ttgacggcgc
     3841 aacccatcac agcaaccttg atgttcttat caaggttccc taacctctct tcaacttcgt
     3901 tagcaatctt tatcagatcg atttgggttc ttccgcatgt agggcatgaa atgaattcga
     3961 taccgccctc taaaagcccc agggatttta agatctccct accaaccgcg atttcctcga
     4021 cgggatcacc ggtaagggaa accctcaggg tatcgcctat gcccatggcc aatagagcgc
     4081 cgatccccac gctggatttt accgtccctc tctttacggt cccggactct gtaacaccta
     4141 tgtggagggg ataatccgtc ctctcggaaa gcagcctgta tgattcaatt gtctccatga
     4201 catcggatga tttaacagat atagcgacat ccccgaaatc taactcgtcc agaatctcaa
     4261 catgcctcaa ggcactctct acaagggctt cgggacatac ctgtccatac tttttaagga
     4321 tgtccctttg cagcgatccg gaattaaccc cgatcctcat gggtataccc ttatccttag
     4381 ctgcctctac taccgccttg accttttccc tgctgccgat attcccggga tttatcctga
     4441 gcttttgggc tccgttctct atggagctta gggcaagcct gtaatcaaaa tggatatcgg
     4501 ctactaaggg aatgttaatc tgatttacta tatccctgag ggcctcagcc gcttccatat
     4561 ccgggactgc gcaccttatg atatgacaac cggcatcctc caggtctctt atctgagata
     4621 ccgttgagcg tacatccctg gtatccgtat tggtcattga ttgaatactt ataggcgaat
     4681 ctccgcccaa ggccacttcc ccaacccata tcttcctggt ttttgcccta gtcattatcg
     4741 atttcccttc ctaactcttc aagtttctcc atcacttcgt taataaccca atcaggggta
     4801 gatgctccgg cagttacacc gaccttttct accccctcag ataacatttt ctttacggcc
     4861 tccgctgata gctcagaact attctctata aatatggtgt tcccgcaatt tttactgcag
     4921 atctcgtaga gctttttgct gttagagcta ttcctgcccc cgataacgat catcagccct
     4981 acatccttcg atacctcata ggcacttgcc tgccgctcct tggtcgcact gcagatggtg
     5041 ttaaaggaca gtacatcttc gcatttttct gaaactactt taagagcttt ttcgtagttg
     5101 gacagtctct cggtggtctg agacactata cagactcttt ggggcagctt atccaggtcg
     5161 cttccgtccc tggttatgac ggcgctatta taacaccacc cattgatacc tatgacctcg
     5221 ggatgatcct tattgccgac gattataatc tgatagccct tatcgtgata cttccgggcc
     5281 ttcctctgta tgctggtaac atagggacat gtggcatcga ttattttagc accggtgttc
     5341 cggatttcgt ctatggtttc gggggttact ccatgggatc tgatgacaat agtatcatct
     5401 tctaccagct tgtctatctc atcaaaactc agcggatata tcccctcttt cctcagggaa
     5461 tccacgacat cattgttatg gattagaggt cctaaggtat acgttctccc cggattcccc
     5521 ccctcccttt gcttgatggt aatgtcaacg gcccttgtga caccaaaaca aaagcctgcg
     5581 ttttccgcaa caattatttg cataggtggg tttcccttgt aacaatttta tcgagtatct
     5641 gggcagcctt taaaatgaca taattgcatt tttcttcttc cgacctatac tccttcttta
     5701 agggcgtaca gtcgatatac cccatctcca tcccatactc cttaaatagc tctctggtca
     5761 acgccccaat tttatcgctc tcgtgggcct tttcctttac aaaaagcttc cctaacacca
     5821 tcaaggcccc ggttagagcc ccgcatttat cctcaatggc cattccaccg ccaaaagctg
     5881 cggacaactt taacgcctct ttatcaagac ccagcttgta tgcgacattt gccccatata
     5941 agatcttctc ggcacaattt aggccttcct cttcaccaaa tccgttcttt ataagttcac
     6001 t
//
LOCUS       DASRNX010000021         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_481487,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000021 DASRNX010000000
VERSION     DASRNX010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481487"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..2075
                     /locus_tag="VFD89_00765"
     CDS             282..2075
                     /locus_tag="VFD89_00765"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZJ56756.1"
                     /translation="MARMRTPLIHLNDIKVRNKLLSLYIFSVLIPIILTNFIFYRKIS
                     NSVREQEMQYIAQDLQKIKGDFKRIIDDNATLSHAIYTDRNLYAFLDRHYEDSHEYYA
                     YYTQYLKNMQERYKFTFPNIYDINIYTDNPGVLDSAGYLRLTEEVRDSDWAQKLQNIQ
                     NQVLVYAHIQKDYNGLHRVFSIIRKLDYYDKWDSYEKILKIDLQFNAVARIIEDRRVD
                     GQIFLVNDANKIIYSSHDSYSPDGSEQFMDFGQIRHNEKDLVMVESFDSITTLHDWRI
                     VGVFSRQKILDALRESATFVVTSAGINLLVASFIIYLIGKSFHHRIQILSGHMEKIRN
                     QDFEVIHYEGGKDEIGQLIRDYNRMVTRIGELIQDVYKADIQRQAMELENKQAQLDAL
                     QSQINPHFLFNTLETIRMRSLVKDEKETAEIIKYLSRTFRRALTWGNDWVTVEEEVQF
                     VESFLRIQQYRFEKRINYIISMEPGTEKLKIPKMTLQPLVENASIHGIEMIKDQGVIE
                     LDVRATGDRLQLMVTDNGVGIDKDRLDQIMKELKEQELNKEHIGIANVYWRLYLYYGE
                     AFRFDIESGENKGTKVTISIPLKYEPRIYGA"
     gene            2088..>2769
                     /locus_tag="VFD89_00770"
     CDS             2088..>2769
                     /locus_tag="VFD89_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZJ56757.1"
                     /translation="MLFRVLLVDDEPFVREGIKKLINWEDCGFILCGEAENGEEAVEL
                     IESLSPHVIITDIKMPVLDGLELIKKVNIEMEKKIKFVILSGYNEFHFAQTAMRYNVK
                     NYILKPIDEDELSGLLKRLYREITNEMRLEKEHALGVDAMANSAVKKIIKGEGNPEVY
                     QQAAELLQIYGDEPICYITIEQDHYKNRLSGLDPVELENRGFHVRQLLVDIIGEENAR
                     NLFEEGDFR"
BASE COUNT          887 a          518 c          671 g          693 t
ORIGIN      
        1 acaaaggtaa tggactattg aaaagcgaat gacttatatt gatttggcta agaaaacttg
       61 acttatggaa tgcagaaaag ccgtagttta aggcaggacg ccttaaaccg attccaccgg
      121 caggaggccg gattggaagc agtaggcttt tcaaatggaa taggccttag ttttctccaa
      181 atcaatgttg gaagtagcgt ttaatagcca ttaccagtat atttatctag cacaagggat
      241 taaattaaca caacacaact tctgcgaggg ggtgattaag aatggcaaga atgaggactc
      301 ccctgattca tttaaacgac attaaagttc gcaataagct gttatcgctg tacatatttt
      361 cggtgctcat tcctattatt cttactaact ttatctttta tcgcaagatt tctaacagcg
      421 tcagagaaca ggaaatgcaa tatattgccc aggacttaca gaaaattaag ggggacttta
      481 aacgcataat cgacgataac gccaccctgt cccatgccat atataccgat aggaacctct
      541 atgccttttt ggacaggcat tatgaggatt cccatgaata ttatgcatac tacacccaat
      601 acttaaagaa catgcaggaa aggtacaaat ttaccttccc taacatctat gacattaata
      661 tctatacaga taaccccggg gtcttggatt cagcgggcta tttaaggctt acagaggaag
      721 tgcgagacag cgattgggcc caaaagctgc aaaacataca aaaccaggtc ttggtttacg
      781 cccatataca aaaggattat aatggattac accgggtatt tagcatcatt cgcaagctgg
      841 actattatga taaatgggac agctatgaga agatattaaa gatagatttg caattcaatg
      901 cagttgcccg gattatcgaa gatcggcggg tggacggtca gatattttta gtcaacgatg
      961 cgaataagat catctactcc tcccatgatt catattcgcc cgacggctct gaacaattca
     1021 tggatttcgg tcaaatcaga cataacgaaa aggatttagt catggtggaa agctttgata
     1081 gtatcactac cctgcatgat tggagaatcg ttggggtgtt ttcccgccaa aagattctag
     1141 atgccctgcg ggaatccgct acctttgttg ttacatccgc cggtataaac ctgttagtag
     1201 cgagttttat tatatatcta ataggcaagt cctttcacca ccggattcag atactttcgg
     1261 gacatatgga gaaaatacga aatcaggatt ttgaggttat ccattatgaa ggcggcaagg
     1321 atgagatcgg ccagctgatc agggattata atcgaatggt cacccgaatt ggggagctga
     1381 tacaggatgt atacaaggcg gacattcaac gtcaagctat ggaactggag aataaacagg
     1441 cccaacttga tgctcttcaa agccagataa acccccattt tttgttcaat accttagaga
     1501 ccattaggat gcgctccctg gtcaaggatg agaaggagac ggcggagatc atcaaatatc
     1561 tgtccaggac ctttcggcgg gctcttacct ggggcaacga ttgggtgacg gtggaggagg
     1621 aagtgcaatt tgttgagagt ttcctaagga tccaacaata ccggttcgaa aagaggatca
     1681 actatatcat atcgatggaa cccggaaccg agaaacttaa gatcccgaag atgaccctcc
     1741 agcccttggt ggaaaatgca agcatccacg gcatagagat gattaaagat cagggcgtta
     1801 tagaattgga tgtcagggcg acaggcgaca gactgcagct tatggtaacc gataatgggg
     1861 ttggtataga caaggacagg ttggatcaaa tcatgaagga gctaaaggaa caggagctga
     1921 acaaggagca tattggtatt gccaatgtct attggcgcct gtatctgtat tatggggaag
     1981 ccttcagatt cgatatagag agcggcgaaa ataaggggac aaaggtcacc atatctatac
     2041 cactcaaata tgaaccaaga atttatgggg catgaaagga gtaatctatg cttttcaggg
     2101 ttttattggt agatgatgaa ccctttgtaa gggaagggat aaaaaaactc atcaattggg
     2161 aagactgcgg atttatatta tgcggtgagg ccgagaatgg ggaagaggct gtggagctca
     2221 tagagtccct ctccccccat gtcataatta cagatataaa aatgcctgtc ttagatgggc
     2281 tggaactgat taaaaaggtc aatatagaaa tggaaaagaa aataaaattt gttatcttga
     2341 gcgggtataa cgaatttcat ttcgcccaga ccgctatgag atataatgtc aagaattaca
     2401 tcctaaagcc catagacgag gatgaattgt cggggctgct caaaaggcta tacagggaaa
     2461 taacaaacga gatgaggttg gagaaggaac atgctcttgg ggtagatgcc atggccaaca
     2521 gcgctgtcaa aaaaataatc aagggggaag gaaacccgga agtctatcag caagccgcag
     2581 agctgctcca gatctatggg gatgagccca tttgctatat cacaatcgaa caggatcatt
     2641 acaaaaatag actaagtggt ttagatcccg tggaactgga gaatagggga tttcatgtaa
     2701 gacagctgct ggtggatata ataggggagg aaaatgcgcg aaacctgttc gaagaagggg
     2761 attttcggt
//
LOCUS       DASRNX010000022        12393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_428122,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000022 DASRNX010000000
VERSION     DASRNX010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12393
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428122"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1214)
                     /locus_tag="VFD89_00775"
     CDS             complement(261..1214)
                     /locus_tag="VFD89_00775"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HZJ56758.1"
                     /translation="MVNIKYYLILLICMFLSLGLGMVIGISLQNKNVLEKQHDIIAQR
                     LEDEFSNMRNENRQLKESLKNFEKLESDNARLYGSVFNSIIRNKLMGLKITLIETGGE
                     DDYAGIIDLLKISGASIDSSIVFDATLFKNEYELDNAINVSSQAAIDKGQLYGKLAEN
                     VIVTLAKGELTPLIQRLTRLNLIRSPVGIQNGCDTIVLAHTGGDNTREGVEKFVYNLI
                     ELIQDYNVNLVVVGKEQDNSLDADWYKNKGISTIDHVDTPYGQLSLISLLYGSQGNYG
                     YGAGTDGILPNELFPQKSEARSMDGLDGEKDDISAFEEEAE"
     gene            complement(1246..2367)
                     /gene="steA"
                     /locus_tag="VFD89_00780"
     CDS             complement(1246..2367)
                     /gene="steA"
                     /locus_tag="VFD89_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HZJ56759.1"
                     /translation="MILKGVAKKNIRTKALITRLTPNDIAVIDHRDIDGVAAIQLADK
                     KIKAVINADRSISGKYPNRGPKILSNAGIPIIDNVGQNVFDKIMDNDTLEISNRGLFV
                     NGNFLCTIACLNKEDIEIKMDKAASNLWTVLDSFIENTLEYAKREKELIINPIELPNS
                     SIDMNGRHVLVVIRGESYREDLRAVQVYIDEVNPIMIGVDGGANALLDFGYRPDIIIG
                     DMDSVSDASLKSCKEIIVHAYRNGYSPGMRRIEDLGLKAIKFPFTGTSEDAALVLAYE
                     QDAELIVTVGSHTNMIDFLEKGRPGMASTMLIQMKVGHKVVDAKGVSELYKNRIRPGY
                     IAAVFAATLFPLSVVAKTSPLIKEIYRLISLRLRILFGL"
     gene            complement(2628..3455)
                     /gene="spo0A"
                     /locus_tag="VFD89_00785"
     CDS             complement(2628..3455)
                     /gene="spo0A"
                     /locus_tag="VFD89_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HZJ56760.1"
                     /translation="MAKKISVLIADDNKDFCEIIDEFLRKQDDIEVIGVANDGIEAVD
                     KILKLKPDAVVLDIIMPHLDGLGVLERLNEENLDVYPKIIILSAVGQDKVTQRAIMLG
                     ADYYIVKPFDLGLLMRRIRELAHDSESGYTSRSTSMIIENTRLPYSKASVSNRNISLE
                     SEIANIIHGIGVPAHIKGYQYLREAIMMVVNDVDLLSGITKELYPGIAERFNTTSSRV
                     ERAIRHAIEVAWSRGRIETINKLFGYTIHDEKGKPTNGEFIAMVADKLRMQAKYLGE"
     gene            complement(3644..4948)
                     /gene="spoIVB"
                     /locus_tag="VFD89_00790"
     CDS             complement(3644..4948)
                     /gene="spoIVB"
                     /locus_tag="VFD89_00790"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HZJ56761.1"
                     /translation="MGKSSFKKICGLIFALFFLLFNYSPYVQGIKRIPNRVQLFEGDT
                     KTLNFRIPLPVKIEGDNVDVLKFNGNSLKDQYVYEMGKPISIKPANSGDVSLDFKLFG
                     LISIKQMLVKVTPPKLIYPGGSSIGVSLYTKGALLVGISEVYDKKGVANNPALDAGLR
                     PGDIIERVNGISIKNAEHLSELVNIAKHQGIELEILRGNQFFVTNIEPVEDYHDGKYR
                     LGIWVRDSTAGVGTLTFYDPEKNHFGALGHAITDVDTGTMLSIKNGEIVQSKIIDIKQ
                     GKRGNPGELKAAFSSKRKVIGYIVKNSLYGIYGKMDKAFIPDTLGEPIPICYKDNVKL
                     GPAKILSTIDEEGIKEFDVQVIKINDQNQASAKGMVIEIVDPRLLDRTGGIVQGMSGS
                     PIIQGGKIIGAITHVFVNDPKKGYGIFIEWMMDESNKIIDSN"
     gene            complement(5171..6904)
                     /gene="recN"
                     /locus_tag="VFD89_00795"
     CDS             complement(5171..6904)
                     /gene="recN"
                     /locus_tag="VFD89_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZJ56762.1"
                     /translation="MLMHLSVRNIALIDEINLDFHNGLNILTGETGAGKSIIIDAVNL
                     VLGERADRDLIQTGKDYALVEAIFDIRGQRDLDNILDELGIEIESDGTLLLMRELAAN
                     GRNICRINGRIVTLSALKNVSKYLIDIHGQHQHQSLLNVDQHLELLDMLGGDMILNSR
                     KEVESIYHTWKGIHKRIRKLSGIGMDGERRKDILSYQIQEIENANLGDNEEEELGNQR
                     AILANSEKIIHIINSIYQNLYTGSNIHLSVTDLIGEALSDLNQIAGIDPKLDELNEKI
                     ESFSYILEDIVSEIRDYREQIEYDPSRLDEVEERLEVIRALKRKYGASIPEITTYLQD
                     IKRELEDLENSRGLLEELAKEEDEILRRLLKASDGLSKKRRKAASFFEQQLLIQLGEL
                     GMDKSNFLVNIGPNGNSEDADRIVNRITSRGYNTVEFLISTNPGEPVKPLSKIISGGE
                     MSRTMLAFKTILAQVDDIPTLIFDEIDVGISGRIAHVIGEKMGSISRSHQVICVTHLP
                     QIAAMADVHYRVQKEVVDHHTRTNVCRLDMAGRQEEIAKMSGGKKLTKASMEHAMELI
                     QAARDYKNKSI"
     gene            complement(6953..7402)
                     /locus_tag="VFD89_00800"
     CDS             complement(6953..7402)
                     /locus_tag="VFD89_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HZJ56763.1"
                     /translation="MKYNRHSKILEIIENTDIETQEELAEELRKKGIDVTQATVSRDI
                     KELRLVKVMAASGTSKYAFIDNTETGISQKLIWVFAESVTSIDSSNNLIVIKTIAGSA
                     QAAASAIDSLGWEEIVGCIAGDDTIMVVIHEDQPVKEIIDRFQQIGR"
     gene            complement(7451..8296)
                     /locus_tag="VFD89_00805"
     CDS             complement(7451..8296)
                     /locus_tag="VFD89_00805"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZJ56764.1"
                     /translation="MKTIGLIANLSKDPCGAKTREIVEIAHSQNMKVQVIPEVYDAIK
                     RGEPTSETELFSSSDIIISLGGDGTLLQAACKAALCDKPVMGINTGNLGFLTNEDFSH
                     ADRALKSLKKGHYSIDKRMMLEGTIRREGKSGTPFFAFNDVGIMKALVSRIIRLKASV
                     NNHEINSYSGDGLLISSPTGSTAYSLSAGGPIVDPSLECLLLTPICPHSLNARSIIIN
                     YSDIIEVEIMSPDRDITLTADGQAVTTLVRGDRILVRKADIYVKLLRTRGQNFFDLVH
                     EKIKI"
     gene            complement(8402..9232)
                     /locus_tag="VFD89_00810"
     CDS             complement(8402..9232)
                     /locus_tag="VFD89_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HZJ56765.1"
                     /translation="MSTTSKQRLDLMLVEMGLAASREKAKRIILSGIVRVDGQRVDKS
                     GTFVSPEAKIFVEEDPVPFVSRGGLKLQKALETFKPPVKDRIYIDVGASTGGFTDCLL
                     KFGAKKVYSIDVGYGQLAWKLRQDHRVVVMERTNIRDVTGKDLEDTPQGAVVDVAFIS
                     LRIVLPVINEILLPDSHIISLIKPQFEAGREKVGRKGVIRSPEVHREVLNNVLSTANQ
                     LDLTLKGLTFSPIKGPKGNIEFLLHLYKGHIGDERPLVDTVEHIRLIESVVKTAHGQL
                     "
     gene            complement(9204..11129)
                     /gene="dxs"
                     /locus_tag="VFD89_00815"
     CDS             complement(9204..11129)
                     /gene="dxs"
                     /locus_tag="VFD89_00815"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZJ56766.1"
                     /translation="MIKYSLLDLIDGPMDLKMLTTDDMNQLSEEIRAFILDSVSNTGG
                     HLASNLGVVELTLSLHYVFSSPKDKLIWDVGHQSYVHKILTGRKGMFDSLRQLGGLSG
                     FPKPEESEHDIFGTGHSSTAISAALGMSRARDIRDCGKYHVVAIVGDGALTGGMGFEA
                     LNDAGHSKTNLIVILNDNEMSISHNVGALAVHLSKVRSSSRYNWFKKGIERMLDRIPW
                     IGKPVSKSIELVKRGFKSLLVHGMIFEEMGFKYLGPIDGHNINSLVNVLGRAKKMDGP
                     ILIHIITQKGKGYEYAEKDPEFFHGVPPFTTETGRTKSMAHETFSWAFGNKLAELAGK
                     DDRVVAITAAMPESTGLLKFRDRFPNRFFDVGIAEQHAVTMAAGLANSGLKPYVAVYS
                     TFLQRAYDQVLHDVCIQNLPVRFGIDRAGLVGEDGETHQGVFDISYLNHIPNLTIFAP
                     KDTQELRDMMDFSLHYERPMAIRYPKGYVNYSVAPYFKAEMPFNPFDWELLIQGRDCC
                     ILSFGKMLGVAVETADLLAKQGIHAQVVNARCIKPLDNNMLKSITLSHNRWITLEDNV
                     ITGGFGSSVVNYIADKGLDIDILNLGIPDAFVPQGKIEQLMSILHLDPGSIANRVSDF
                     LNGIKENVYVHNFKTKA"
     gene            complement(11126..11743)
                     /locus_tag="VFD89_00820"
     CDS             complement(11126..11743)
                     /locus_tag="VFD89_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56767.1"
                     /translation="MPKSVRTKRNNKKKGGSFKNHWVLKIFFITFAISVVLSIISQKA
                     FSSSDGLLLPLFIILVIIFVGVIFDIIGISVASAPETPFLSMSAKRIKGAKQALRLIK
                     NADIVSNFCNDVVGDICGIISGAAGSMLVLKMIFLGISPDPLTILISSLIAAITVAGK
                     AIGKSLAINNSQRIVYGVGYLLSFFSTNGFKKNRDSRMTKRKEAK"
BASE COUNT         3257 a         2974 c         2194 g         3968 t
ORIGIN      
        1 caacaagaat atcttttatc atatggattt tttttagagt ccttagggtc gccgccaatc
       61 ttgttgcctc gttataggct ggaatgatta cgcctacatc ctgtgatagc atatccatcc
      121 tccaaatcaa tcctttttag cttcttttca ataatcgcgt ctatctatga attctttcat
      181 catgtaattg catagaccca tcgaaatcat cctgccaata aatttagttt ccgtattgtt
      241 aatctgcaat agcgatggaa ttattccgcc tcctcttcaa aagcgcttat atcatccttt
      301 tccccatcaa gtccgtccat agatcgggct tcagattttt ggggaaatag ctcattgggc
      361 aaaataccat ccgtacccgc cccataccca taattccctt ggcttccgta tagcaaagat
      421 attagcgaga gttgcccata aggggtatcc acgtgatcta tggttgaaat acctttgttt
      481 ttataccagt ctgcatctag cgaattgtct tgttcctttc ctaccacaac caaatttaca
      541 ttataatcct gtatgagctc tattaggtta taaacaaatt tttctacacc ttccctcgta
      601 ttgtccccac ccgtatgggc caacacaata gtatcgcagc cattttggat cccaacggga
      661 ctacggatta agttcaacct ggtcaagcgt tgtataaggg gtgtcaattc ccccttggcc
      721 aaggttacta tgacgttctc cgccaatttt ccgtacaatt gacctttatc tatagccgct
      781 tgggaggaaa cgttgatagc attatccaat tcgtattcat tcttgaacaa tgtggcatca
      841 aaaacgatgc tgctatcgat tgatgcacct gatattttca gcaggtcaat tatccccgcg
      901 taatcatcct caccccccgt ttctataagg gttattttta atcccataag cttatttctt
      961 ataatagaat tgaatacaga tccatataac ctagcgttat cactttccaa tttctcaaaa
     1021 ttctttaggg attcctttag ttgtcgattt tcatttcgca tatttgaaaa ttcatcttca
     1081 agccgttgtg cgattatgtc atgctgcttt tccaatacat ttttgttctg taacgatatc
     1141 cctatgacca ttcccagccc tagggaaagg aacatgcaga taagtaaaat aagatagtat
     1201 tttatattta ccattaagtc caccctctac aatgtcattt catacctaca aaccaaagag
     1261 tattcttaaa cgcagggata ttagcctgta tatttcctta attaagggcg atgttttggc
     1321 aactactgat agaggaaata gggttgcggc aaaaactgcg gcaatgtaac ccggtcttat
     1381 tcgattctta tacagctcgc tgactccctt tgcatcaaca actttatggc ccaccttcat
     1441 ctgaatcagc attgtactgg ccattccagg cctacccttt tcaagaaaat ctatcatgtt
     1501 ggtatggcta cccaccgtta caatgagttc ggcatcctgt tcgtatgcta aaaccaaagc
     1561 ggcgtcctcg ctggtaccgg tgaaggggaa cttaatcgct ttgagcccca gatcctctat
     1621 tctcctcatc ccggggctat agccgttccg atatgcatga actattatct ctttgcaact
     1681 ctttaggctt gcatcgctga cgctatccat atccccaata atgatatccg gacgataacc
     1741 gaaatccagc agtgcattgg ctccgccgtc aacccctatc attatgggat tgacctcatc
     1801 tatataaacc tgaaccgccc ttaagtcctc cctgtagctc tcgccccgaa ttactacaag
     1861 gacatgcctg ccattcatat ctatggacga attaggcagc tctatcggat tgataattag
     1921 ctccttttcc cttttggcat actctagggt gttctctata aaactatcca gtacagtcca
     1981 taggttagat gcagccttat ccatctttat ttctatatcc tctttattta agcaggcaat
     2041 tgtgcataga aaatttccat tgacgaacaa gcctctatta ctgatctcta aagtatcgtt
     2101 gtccataatt ttatcgaaca cattctgccc cacattatct attatcggga tacccgcatt
     2161 tgataggatt ttaggtcccc tgttgggata tttaccgctg atggacctat ccgcgtttat
     2221 gacagccttt atctttttgt ccgctaactg tattgctgcg acaccatcga tgtctctatg
     2281 atctataacg gcaatatcat taggagttaa tcgggtgatt agggcctttg ttctaatatt
     2341 cttttttgca actcctttta gtatcatctc atccctacac ctctttagta tcgcttatta
     2401 tctatcattt acaaatatct tctaaatatg cataaaaaaa cagccccgga acaagggctg
     2461 attttctgaa catcctctat atgatgttat aacatcggaa gcaattgcat tggcaattgc
     2521 aacacacagg tttcaacggg tcgagggatt tatacccatc gcctgttata ctttactaat
     2581 cggaactcgc cgcttataga tgcaggggat attttgccga ctcgttatta ttcacctaaa
     2641 tactttgcct gcattctcag tttatcggca accatagcta taaattcgcc atttgtcggt
     2701 tttccttttt catcgtggat agtataccca aataatttat ttatggtttc gatacgaccg
     2761 cggctccaag ccacctctat agcgtgacgg attgctcttt ctactctact ggatgtagta
     2821 ttgaatcttt ctgctattcc gggatataat tcctttgtta ttccactcaa tagatccaca
     2881 tcattaacta ccatcataat tgcttcccgt aaatattgat atcccttaat atgcgcaggt
     2941 acccctatcc catggataat attcgctatc tccgattcaa gggaaatatt cctgtttgat
     3001 acggaagcct ttgaatatgg gagtctagta ttttctatta tcatactggt acttctgctt
     3061 gtataaccac tttccgaatc atgggctaac tcccttattc gcctcataag caacccaaga
     3121 tcaaagggtt tcacgatgta gtaatccgcc cctagcatga ttgcccgttg tgtaactttg
     3181 tcctgtccta cggctgatag aattattatc ttgggatata cgtccagatt ctcttcattt
     3241 aatctctcca aaacacccaa tccgtccaaa tgcggcataa ttatatccag tactaccgca
     3301 tcaggtttta gttttagtat cttatcaact gcttctattc catcattagc cacacctatt
     3361 acctctatgt catcctgctt gcgtaaaaat tcatcgataa tttcacaaaa atccttgtta
     3421 tcatcagcta ttaacactga gatcttttta gccatatagc taaccccccc ccaaatattt
     3481 acttatatct tttcatatac ccaaaaaaat ctgaccgtgt ataccaaggc gtccattttt
     3541 ctccattagt aaacatattc gacaatgaat tggattttcc ttcctattat cgataaatat
     3601 ttatactctt ctaaattata ggtaaattat tcgcttcttt agatcagttg gagtcaatta
     3661 tcttattgga ctcgtccatc atccattcaa tgaatatgcc ataccccttt ttgggatcgt
     3721 taacaaatac atgggtgatg gccccaatta tcttgcctcc ttggatgatt ggactgccgc
     3781 tcattccctg aactattccc cccgtccggt ctaagagccg cggatccaca atttctatta
     3841 ccattccttt ggcactggct tggttttgat cattaatctt tataacctga acatcaaatt
     3901 ctttgattcc ctcttcatca atcgtagata aaattttagc cggtcccaac ttgacattat
     3961 ccttgtaaca tattggtatg ggttctccca aggtatcggg gatgaaggct ttgtccatct
     4021 ttccatatat tccatagagg gagtttttaa cgatatagcc gattactttt ctcttgctgg
     4081 aaaatgcagc ttttagttcg ccgggattcc ccctcttccc ttgttttata tctattattt
     4141 tagattgaac aatttcaccg tttttaatcg atagcatagt ccctgtgtcg acatcggtaa
     4201 tagcatggcc tagggctcca aaatgatttt tttcgggatc ataaaaggta agagtaccca
     4261 cacccgcggt actatcccta acccatattc ccagtctgta ttttccatca tgataatctt
     4321 caacaggctc aatatttgta acgaaaaact gattacctct caagatttcc agttctattc
     4381 cctgatgctt agcgatattg accagttcag aaagatgttc tgcgtttttt attgatattc
     4441 cgttgaccct ttctatgata tcacccggtc gtagtcctgc atcaagggct ggattatttg
     4501 ctacgccttt cttatcatag acctctgata tccccacaag cagggctccc tttgtatata
     4561 atgatactcc tatggaactc ccgccggggt atataagctt tggcggggtg acctttacga
     4621 gcatttgctt tatggatatc aacccaaaga gtttaaaatc caaagataca tcccccgagt
     4681 tggcaggctt aatcgatata ggtttgccca tctcatagac atattggtcc tttaaagagt
     4741 tgccgttgaa ctttaaaaca tcaacgttat cgccttctat ctttaccggt aatggtatcc
     4801 taaaattcag ggttttggta tccccctcaa acaattggac cctattggga atgcgtttga
     4861 taccctgaac ataaggtgaa taattgaata gcagaaaaaa taatgcaaaa attaaaccgc
     4921 atattttctt aaaagatgat ttccccatac tctagtattc tcctgtctca acttttatgt
     4981 tttcgttttg tttatctata cataaggtaa cctcataaaa gtgatttata cgagttaaga
     5041 atgcgtaaaa ttagggaaaa aaagaaaggg atatgctccc tttgattttt caaagattat
     5101 tttggatttc aattgtgttt catgaagccc ttggcaggtt tgccagcctt gatttgatgt
     5161 ccctttgcgg ctaaatagat ttgtttttat agtcccgggc agcctggatt aattccatag
     5221 catgttccat actggctttt gtgagtttct ttccgcctga catctttgcg atctcttcct
     5281 ggcgaccggc catatctaac ctacacacat ttgttctggt atggtggtcc accacttctt
     5341 tttgaacccg gtaatggaca tctgccattg ccgcaatctg cgggaggtgg gttacacaga
     5401 ttacttgatg cgaacgagat atactgccca tcttttcacc gatcacatgg gcgatacgcc
     5461 cgcttatccc aacatctatt tcatcgaaaa ttagggttgg aatatcatcg acttgggcca
     5521 atatagtctt gaatgcaagc atcgttcgtg acatctcacc gccggagatt attttcgata
     5581 aaggctttac cggttccccg ggattggtgg aaattaagaa ctccaccgtg ttatatcccc
     5641 tagaggttat tctattaact attctatcag catcttctga gttaccattt gggcctatat
     5701 tgacgagaaa gttggattta tccatcccaa gttcgccaag ctggataagc agctgctgtt
     5761 caaaaaatga agcagctttt cttcgtttct ttgatagccc atctgatgct ttaagtaatc
     5821 tgcgcagaat ttcatcttct tccttcgcaa gctcctctaa tagaccccga ctgttctcca
     5881 gatcctctaa ttctctttta atatcctgta ggtatgtcgt tatttcgggg atacttgcgc
     5941 catattttct ttttagagct ctaattactt ccagacgttc ttctacttca tccaatctgg
     6001 atggatcgta ttcaatttgt tccctataat cccttatttc cgatacgata tcctccaaga
     6061 tatacgaaaa ggattctatt ttttcattta attcatccaa tttagggtcg ataccggcaa
     6121 tttgatttag gtccgataat gcttctccaa taaggtccgt aacggagaga tgaatattag
     6181 acccggtata gagattctga taaattgaat ttatgatatg gattattttt tccgaattgg
     6241 ctaatatagc cctttgattg cccaattctt cctcttcgtt atctcccagg ttggcatttt
     6301 caatctcttg aatttggtag gacaatatat ctttacgcct ttcaccatcc ataccgatac
     6361 ctgaaagttt tctgattctt ttatgtatcc ccttccaggt atgatatatg ctctctacct
     6421 ctttccttga gtttaggatc atatctccgc cgagcatatc caataattcc aaatgctgat
     6481 caacgtttaa aagtgactgg tgctggtgct gaccatgaat atcgattaga tatttgctta
     6541 cattctttaa agcggacaaa gtgacgattc tcccattaat cctgcatatg tttcttccgt
     6601 tggcggccag ttcgcgcatt aatagcaggg tgccatcgga ttcgatttct ataccgagtt
     6661 cgtccaaaat gttgtctaaa tccctttggc cccttatatc aaatatagct tctaccaatg
     6721 cgtaatcttt ccccgtttgg attagatccc tgtctgccct ctcccctaat actaagttaa
     6781 cagcatcaat gataatggat ttacctgccc cggtttctcc ggttaaaata ttaagtccat
     6841 tatgaaaatc caaattgatc tcatctatta gagcaatatt tctaaccgac aaatgcataa
     6901 gcatccaaca gtcccccttg tagaattcca tggtctgttt tcttttccgg cctcagcgtc
     6961 caatttgttg aaatcgatca attatctctt taaccggttg atcttcatgt atgaccacca
     7021 tgatggtatc atcaccggca atacaaccga caatctcttc ccaacctagc gagtcgatag
     7081 cagacgcagc agcttgggcg cttcccgcaa tagttttaat cacaatcaaa ttattagatg
     7141 aatcaatcga tgtaacggat tctgcgaata cccagatcag tttttgggaa atccctgttt
     7201 cggtattatc aatgaaggca tattttgagg tgccagaggc agccattact tttacaagcc
     7261 taagctcctt aatatccctg gagacggtag cctgggtgac gtcaatccct ttttttcgta
     7321 attcttctgc caattcttct tgggtttcaa tatccgtgtt ttcgatgatc tctaaaattt
     7381 tagaatggcg attatatttc atattggctc atacctccat aaccctattc tttgccgata
     7441 ctttcatccg ttatatctta attttttcgt gaactaaatc aaaaaagttt tgccccctgg
     7501 tgcgaagcaa cttgacatat atatctgcct ttcttaccaa gatcctatcg cccctgacca
     7561 acgtagtcac ggcttggcca tctgctgtta aagtgatatc gcgatcaggg ctcattattt
     7621 ctacctctat tatatcacta tagtttatga taatcgagcg tgcattcaat gaatggggac
     7681 agatcggagt gagaaggagg cattccaaac tgggatccac gatgggaccg ccggccgaaa
     7741 gtgaataagc tgtggatccg gtagggcttg atatcaatag accatcgccg gaataactgt
     7801 tgatctcatg attattgaca cttgctttta atcgaattat tctggaaacc aacgccttca
     7861 ttattcccac atcgttaaag gcgaaaaatg gagtccccga ttttccctcc ctgcgaatgg
     7921 taccttccaa catcattctc ttgtctatgg aataatggcc cttctttaat gactttaacg
     7981 ctctgtcggc atgggaaaag tcttcgtttg ttagaaatcc cagatttccc gtattaattc
     8041 ccattaccgg cttatcgcat agggcagcct tacatgctgc ctgaagtagt gttccgtctc
     8101 cccccaagga gattattata tccgatgatg aaaacaattc cgtttcagag gtcggttcac
     8161 cccttttaat ggcatcataa acctccggta ttacctgcac tttcatattt tgactatggg
     8221 caatctctac tatctcacgg gtttttgctc cgcaaggatc cttagataaa ttggctataa
     8281 gtcctattgt cttcaataag cccccccctt tatgggtatt tcatgataga gttgatcaat
     8341 tggcgctcag agttcatgct cttgattcta ttaatagttt ttagtccttt cggagtgttt
     8401 cctaaagctg accatgcgcc gtttttacca cactttcaat gaggcgaata tgctctacgg
     8461 tatctaccag gggtctttcg tccccaatat gtcccttata taggtgtagt agaaattcga
     8521 tattcccctt cggtccttta atcggggaaa aggttaagcc tttgagggtc aggtccaatt
     8581 gattggctgt agataaaacg ttgtttaaaa cctccctatg aacctcgggg gatctgatta
     8641 caccttttct gccgaccttt tccctaccgg cttcaaattg gggctttatt aaggaaatga
     8701 tgtggctgtc gggcaacaga atttcattta tgacaggcag cactatcctc agggatataa
     8761 aggcgacatc cactaccgca ccctgggggg tgtcttccag gtccttccca gtcacatcac
     8821 gaatattagt cctttccatg acgacaaccc tatgatcttg tctcagcttc catgccaatt
     8881 gaccataccc aacatcaatg gagtatacct tttttgctcc aaatttcaaa agacaatcgg
     8941 taaatccccc cgttgaagcc cctacgtcta tgtagatcct gtccttcacc ggaggtttga
     9001 aggtttccaa agccttttgg agcttgagtc cgcccctgct gacgaaggga accggatctt
     9061 cctcaacaaa tattttcgct tcgggagata caaaggtacc cgatttatcc accctttgtc
     9121 cgtcaacacg cacaattccg gacagaataa tgcgctttgc tttttcccgc gatgccgcta
     9181 atcccatttc aaccagcatc aggtcaagcc tttgttttga agttgtggac atatacattc
     9241 tccttaatac cgtttaaaaa gtcacttacc ctattggcta tactccctgg atccaggtgc
     9301 aggatgctca ttagttgttc aatttttccc tggggtacga aagcatcggg aatccccaag
     9361 tttaaaatgt ctatatccaa tcctttatca gcaatataat tgactacaga gcttccaaac
     9421 cctcccgtta ttacattgtc ttcaagtgtg atccaacggt tatggcttag ggtaatggat
     9481 tttagcatat tattatccag aggctttatg cacctggcat taaccacttg ggcatgaatt
     9541 ccttgcttcg ccaaaaggtc agccgtctca acggcaacgc ccagcatttt gccaaaagac
     9601 aatatacaac aatccctccc ctgtatcagc aactcccaat caaagggatt aaacggcatt
     9661 tccgctttga agtaaggcgc cacagaatag tttacgtagc cctttggata ccttatagcc
     9721 atcggccttt cataatgcag ggaaaagtcc atcatatccc tcaactcctg ggtatctttg
     9781 ggagcaaaga tagtcaaatt aggaatatga tttaaatagc taatatcgaa tacaccttgg
     9841 tgggtttccc catcttctcc aactaacccg gcgcgatcaa taccaaacct tacgggtagg
     9901 ttttgaatgc aaacatcgtg aagaacctga tcatatgccc tctgaaggaa ggtggaatat
     9961 acggccacat agggcttcag tccgctattg gcaagccctg cagccatagt aactgcatgt
    10021 tgttctgcta ttcccacatc aaaaaaacga ttggggaacc tatcccgaaa tttcaacagt
    10081 cctgtagact ccggcattgc ggcggtaatt gccactaccc tgtcatcttt gcccgccaat
    10141 tccgccaatt tgtttccgaa tgcccatgaa aatgtttcgt gggccatact cttggttctg
    10201 ccggtttccg tggtgaaggg aggtactcca tggaagaatt cggggtcctt ttctgcatat
    10261 tcatagcctt tacccttttg tgttataata tggataagta tgggtccatc catcttcttg
    10321 gcccggccca aaacgttgac taggctgttt atgttgtgtc catcaatagg tcctaggtac
    10381 ttaaaaccca tttcttcaaa tatcattcca tgtaccaaaa gtgatttaaa accccttttg
    10441 accaattcaa tggatttgga tacgggttta cctatccagg gtatcctatc caacattctt
    10501 tctataccct ttttaaacca attatatctg gaactggacc tgaccttgga taagtgaacc
    10561 gctaatgcac cgacattatg ggaaatcgac atttcattgt cattcagtat aactatgaga
    10621 ttagtttttg aatgccctgc atcatttaag gcctcaaatc ccatgccccc agtaagggca
    10681 ccatcgccta caatagcgac aacatggtat ttaccacaat ctcttatgtc cctggcccta
    10741 gacataccta gcgcagccga tatagcggta ctgctatgcc cggttccaaa aatatcatgt
    10801 tcgctttcct cgggctttgg aaaaccgctc agtccgccca attgtctgag agagtcgaac
    10861 atcccctttc gtcctgtcaa tatcttatgt acatatgatt gatgcccgac gtcccaaatg
    10921 agcttgtcct tagggctgct aaagacatag tgaagtgata atgtcaattc gactacacct
    10981 agatttgacg ccagatggcc gccggtgtta gagacgctat ctaatataaa cgcccgtatt
    11041 tcttcggaca attgattcat atcatctgtt gtcagcattt ttaggtccat gggtccgtct
    11101 attaaatcaa gcaacgaata tttgatcatt tagcttcttt ccttttagtc atcctactgt
    11161 ctctgttttt tttaaaaccg ttcgttgaaa aaaatgatag cagatagcct acgccataga
    11221 caattcgttg gctgttattg atggccaggc tctttccaat agccttgccc gctaccgtta
    11281 tagctgcaat taaactgctt attaaaatgg tcaggggatc aggactaatg cctaaaaaaa
    11341 tcatttttaa gactaacata ctacctgccg ccccgcttat tattccgcaa atatcgccta
    11401 caacatcatt gcaaaagttt gaaactatat ccgcgttttt tattagccgt aaagcctgtt
    11461 tggcgccctt tattcttttg gctgacatgg ataaaaaagg cgtttccggt gcagatgcca
    11521 ctgaaattcc aattatatca aatataactc ctacaaaaat aataaccaat atgataaaca
    11581 agggtaacaa cagaccatcg gacgaagaaa aagccttttg ggagatgatg ctcaatacta
    11641 ctgagatggc aaatgtaata aaaaatatct tcaacaccca atgattttta aagctaccac
    11701 ccttcttttt attattcctt ttggttctga cggattttgg caaacctgcc cctcccatta
    11761 tttaactctt gtagttatca accgggataa aaaaatagtg atctggtggt gatggattgg
    11821 ataaactttg cggctttggt ccagtaggtt ttcccgaacc cgtgcttttc gtcgccgaaa
    11881 aagcggtttc cctttaaacg aattcttatt gtgtcgccaa caataaaact ggatcgccat
    11941 aataccacac tgtaatctcc aattcatccc gtaacaggca gtctaggagc tttcctcggc
    12001 agtccaaacc aatttctagc ctcttttgca aaaaggattt catacggcaa taatctcagt
    12061 tcatgggccc tacgaacgtc gatcggatcc ataatccgcc cattccactc aaggcaggct
    12121 acactgcaca cagctttttc acaccgcaat acaggtttaa tcccaagtag tagtagaaca
    12181 accaccggct tgggtctcca cgaaagctgg gtcaacgccc gcatgccatt gcggatcgcc
    12241 cttctttctt aactcccagc accagccccc aactgggcgt cagatgccag caccaggaac
    12301 ttcatcgatg tgccctcaac ggatttttag gcccgccttc aaaattggca gcactgacta
    12361 gaatactgca ccaccagatt aattattata aca
//
LOCUS       DASRNX010000023         8824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_640471,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000023 DASRNX010000000
VERSION     DASRNX010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640471"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..422
                     /locus_tag="VFD89_00825"
     CDS             117..422
                     /locus_tag="VFD89_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HZJ56768.1"
                     /translation="MVLHLGGEVIIPMKDVIAIFDLETSKDSKINNEFLEIVKDEGFI
                     RVLSEDAPKTFVLTEFDNKITIYLSPISSSTLLKRTGFIQNISFSKKQLEQGGYDGE"
     gene            409..2328
                     /gene="gyrB"
                     /locus_tag="VFD89_00830"
     CDS             409..2328
                     /gene="gyrB"
                     /locus_tag="VFD89_00830"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HZJ56769.1"
                     /translation="MMENNGQRNRNDYDESQIQVLEGLEAVRKRPSMYIGSTSLRGLH
                     HLVYEVVDNSIDEAMAGFCDDISVTIHKDMSVTVQDNGRGIPVGVQEKLQKPAVEVAL
                     TMLHAGGKFGGSGYSVSGGLHGVGVAVVNALSEWLTVKVKRNGHIYTQRYERGITKTQ
                     LEVTGDTQETGTTITFKPDNKIFEELDFSFDTIKTRLRELAFLNAGIRIGIRDERIDE
                     DESFHYEGGIISFVEYLNKNRVAIYSPPIYISGQRGTTSMEVALQYNDSYVEGVYSFA
                     NNIDTHEGGTHLSGFRAAITRVLNDYGRKYNYIKQNEANLSGEDTREGLTAIVSVKLT
                     EPQFEGQTKTKLGNSEIRGFVESTVGEGLSAFLEENPNTGRVILEKALTASRAREAAK
                     KARELTRRKSVLESTSLPGKLADCTERDPALSEIFIVEGDSAGGSAKQGRDRHFQAIL
                     PLRGKILNVEKARLDRILANEEIRAMITAFGTGIDDEFDIEKLRYHKIIIMTDADVDG
                     SHIRTLLLTFFFRFMKPLIEKGHIYIAQPPLYLARKARREHYCYSDNELEELLTEIGR
                     EGVEIQRYKGLGEMNADQLADTTMEPSKRTLLRVAMEDARQADEIFTILMGEKVEPRR
                     EFIEENAKLVTNLDV"
     gene            2346..4787
                     /gene="gyrA"
                     /locus_tag="VFD89_00835"
     CDS             2346..4787
                     /gene="gyrA"
                     /locus_tag="VFD89_00835"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZJ56770.1"
                     /translation="MDPSFNKIIDVNIEKEMSKSFIDYAMSVIVSRALPDVRDGLKPV
                     HRRILYSMFELGMTPDKPFRKSARIVGDVLGKYHPHGDSSVYDAMVRMAQDFSIRYML
                     VEGHGNFGSVDGDSPAAMRYTEARMSRISMELLADINKDTVDFTPNFDETLKEPNVLP
                     SRFPNLLVSGSSGIAVGMATNIPPHNLGETIDGVVALIDDPDISIEEIMNHIKGPDFP
                     TGALILGREGIHQAYGTGRGRIITRAKTQIEEYSGNRTRIIVEELPYQVNKARLIEKM
                     ADLVRDKRIDGISDIRDESDRSGMRIAVDLKRDANPNVVLNYLFKHTQMQVTFGAIML
                     ALVDGQPRIMNLKEILYHYLNHQKEIVVRRTRYELERAEARAHILEGLLIALDHIDEV
                     ISLIRGSRTDQIAKEGLMQKFGLSERQAQAILDMRLRRLTGLERDRLVEEYEGLLKTI
                     RGLKDILGSEDLQYSIIKGELLSVKDRYSDDRRTQIISAPGEIDIEDLIDEHDVVLTL
                     THLGYIKRTPLDAYRSQRRGGRGITGLQTREEDFVFSLFVTSTHQRLLFFTSLGKVYE
                     LKAYEIPEAGRQARGTAIINLLQLMPGERIQAVIPVDEFESGKFLVIGTKNGLVKKSP
                     LEEYENIRRGGLIAVNLRQNDEVVGVQLTDGDREILLATHRGMCIRFNESDVRSMGRA
                     SMGVKGIHLSRDDYVIDMILVSGQDEVLAISENGFGKRTSIDEYRVQRRGGRGIITMR
                     TNEKTGNLVALRMVSEDEDLMIINSEGIIIRIGVKEISTLGRNTQGVMLMRVEEGMTV
                     ISVAKAEKDDDEDAF"
     gene            4819..5892
                     /locus_tag="VFD89_00840"
     CDS             4819..5892
                     /locus_tag="VFD89_00840"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007087853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HZJ56771.1"
                     /translation="MGKIKFIKKDELKVKPKDSELVFGTQFTDYMFVMDYDVKKGWYD
                     PRIVPYGPIEMSPSSMVFHYGQAIFEGMKAYRDNNGDIVTFRAMDNFNRMNRSADRIC
                     MPKVDTGFVWDALNTLLDIERDWVPSSKDTSLYIRPFMIATDPFLGVRPSNTYKFFII
                     LSPVGPYYPEGLDPVKIYVEDKYVRVSKGGTGEAKVAGNYAASLLAAEKAKDKGYSQV
                     LWLDAADHTFVEEVGAMNIFFKIDGKLITAPLDGSILPGITRDSVIKLAKHLGYTVEE
                     RDLRVEDVFAAHDNGTLEEVFGSGTAAVISPVGILEWMDNKIEINDGKIGEFTSRMYD
                     MLTGIQYGTQEDVFGWVNRVKCR"
     gene            complement(6016..6327)
                     /locus_tag="VFD89_00845"
     CDS             complement(6016..6327)
                     /locus_tag="VFD89_00845"
                     /inference="COORDINATES: protein motif:HMM:NF019490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HZJ56772.1"
                     /translation="MAKNQLSEKEMLLDSIMTERYVSSAYDTGVMDSVNQEVTTALQN
                     IQQEEQNHTKLFMEVMHNHGWYDVQGAHINQSAKGQIDQQMAFQLENRIGEIEQNSQT
                     P"
     gene            complement(6346..6561)
                     /locus_tag="VFD89_00850"
     CDS             complement(6346..6561)
                     /locus_tag="VFD89_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZJ56773.1"
                     /translation="MELSDKEKKILEDQLKAEYICMSKYKLYSEQATDPQIKNIFNLV
                     YQQEVQHAETLKSLLEQGGFSPPSPTS"
     gene            complement(6956..8026)
                     /locus_tag="VFD89_00855"
     CDS             complement(6956..8026)
                     /locus_tag="VFD89_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZJ56774.1"
                     /translation="MKTIVLTGGGTAGHVTPNIALIPELLKQGYDIHYIGTENGIERG
                     LITKEKGVTYHVINSGKLRRYMDIQNFTDPFRVIQGVGQSINLIRRLKPAIIFSKGGF
                     VSVPVVLGGWINKVPVIIHESDITPGLANKISFPFAVKICTTFKETTEFFKGDKGVYT
                     GTPLRPGLLKGDPKKGMDICGFTTEKPALMVMGGSLGAVAINQFVRSLLGRLTNRFNV
                     IHLCGNNNLDPTMENIPGYKQFEYVDEDLPHLMALAGVIISRAGANSIYEFLALNKPN
                     ILIPLPLSASRGDQIQNAESFERQGFSKALDQDTLTEDILLTAILDVYANRHRYIDTM
                     ARMDHTKGVERMLELINEYAKL"
     gene            complement(8078..>8824)
                     /gene="argS"
                     /locus_tag="VFD89_00860"
     CDS             complement(8078..>8824)
                     /gene="argS"
                     /locus_tag="VFD89_00860"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZJ56775.1"
                     /translation="NTYDFEKCIYVTGAAQSLHFDQWFKVIELMGYDWYDKLEHVPFG
                     IVSLGGEKLATRKGKVVLLEDILSEAIKKTSRIIGEKNPNMDEDEKNEIARQMGVGAV
                     VFSDLSSNRIKDVSFSWDEILNFDGETGPYVQYTHARTCSLLKRAKMEVDPNFDAGLL
                     LSREEFRVIKTLYFFPDIVVSAMNDLEPSIITRYLVDLAQDFNRFYHEHSILIEDDDL
                     RNARLALVLAVNHTLANGLNLIGLSAPERV"
BASE COUNT         2501 a         1833 c         2218 g         2272 t
ORIGIN      
        1 agcattataa cccgtatttt tgtattaata tttttttgga ccttaccgaa ggggattgat
       61 accgtatgcc gaaatatgcc gagaattcgt aaattgagga gaggtcggtg gaactagtgg
      121 tactgcattt aggtggagaa gttattatac ccatgaagga tgtcatcgct atatttgatt
      181 tggagacatc aaaagattca aaaataaata acgagttttt agagattgtt aaggatgagg
      241 gttttattag ggtcctatcg gaggatgccc cgaaaacctt cgtcttgact gaatttgata
      301 acaagataac gatatattta tcccctattt catcttcgac ccttttaaaa agaaccggat
      361 ttatacagaa catatctttc tcaaaaaaac aattagaaca agggggatat gatggagaat
      421 aacggacaaa gaaataggaa cgattatgac gaatcccaga tccaggtact ggagggattg
      481 gaggccgtac gcaaacggcc tagtatgtat ataggaagta caagtctgcg cgggcttcac
      541 catttggtat atgaagtggt ggataacagc atagatgaag ccatggcagg gttttgtgat
      601 gatataagtg taaccataca caaggatatg tctgtaaccg tccaagataa tggaagggga
      661 attcccgttg gggttcagga gaaattgcag aaacccgccg tggaagtagc ccttaccatg
      721 ctccacgctg gcggaaaatt cggggggagc gggtatagtg tatccggcgg attgcacgga
      781 gtaggcgttg ccgtcgtcaa tgccttatcg gaatggctca cggttaaggt taagagaaat
      841 ggccatatat atacccaaag gtatgagagg ggtataacga aaacccagct ggaggtaacg
      901 ggggacaccc aagaaaccgg taccaccata accttcaaac cggataataa gatattcgaa
      961 gagttggact ttagctttga taccataaaa accaggctga gggagctggc ctttttaaat
     1021 gcggggataa ggataggaat aagggatgag agaatcgacg aagacgaaag ctttcactat
     1081 gaagggggaa tcatctcctt tgtagaatat ctaaataaaa atagggttgc catctatagc
     1141 ccccctatct atatttccgg gcaaagggga accacttcca tggaagtggc cctccaatac
     1201 aacgactcct atgttgaagg ggtatactcc tttgccaata atatagacac ccacgaaggg
     1261 ggaacccatc ttagcgggtt tagggccgcc attaccaggg tgctcaatga ttatggcaga
     1321 aaatacaact acattaagca aaatgaggct aatctttccg gcgaggatac ccgggagggt
     1381 ctgacggcta tagtgagtgt aaagctcacg gaaccccagt ttgaaggcca aaccaagacc
     1441 aagctgggta acagcgaaat aaggggattt gttgagagca cggtagggga ggggctctcg
     1501 gctttcttag aagaaaaccc caacaccggc agggtaatcc tagaaaaggc tcttactgca
     1561 tcaagggcca gagaagcggc caaaaaagcc agggaattga ccagaagaaa aagtgtacta
     1621 gagagcactt cattgccggg gaaattagcg gattgtaccg aaagagatcc ggcgcttagt
     1681 gaaattttta tagtggaggg agattcagcc ggcggttcgg ccaagcaggg cagggatagg
     1741 cattttcagg ctatcttgcc cctcagaggc aagatactga acgtagagaa ggcaaggctt
     1801 gataggatat tggccaatga ggagataagg gccatgatta ctgcctttgg tacgggtatc
     1861 gacgatgaat ttgatattga aaagctgaga taccataaaa taataataat gaccgatgct
     1921 gatgtggacg gcagccacat aagaaccctc cttttaactt tcttctttag atttatgaag
     1981 cccttgatcg aaaaagggca tatatatata gcccaacccc ccctttacct ggccaggaag
     2041 gccagaaggg agcactactg ctacagcgat aatgaattgg aagagctgtt gaccgaaatc
     2101 ggcagggaag gggtagagat ccagcgctat aagggtttgg gggagatgaa cgccgaccag
     2161 ctggccgata ccaccatgga gcccagtaaa cgcaccctcc ttagggtagc tatggaagat
     2221 gccaggcagg cagatgaaat atttacaata ctcatgggcg aaaaggtaga gccccggcgc
     2281 gaatttattg aggaaaatgc taaattggtg accaacctag atgtttaagg aagggatgta
     2341 aatagatgga tccatcattt aataagatta tagatgtaaa tatagaaaaa gagatgtcaa
     2401 aatcctttat agactatgct atgagcgtaa tcgtaagccg cgcccttccg gatgtgaggg
     2461 acgggttaaa gccggttcat aggaggatac tctattccat gtttgaactg ggaatgacac
     2521 cggataaacc cttcagaaaa tccgccagga tagtggggga tgtcttaggt aaataccatc
     2581 cccatgggga ctcttccgta tatgatgcca tggtcagaat ggcgcaggat ttttctattc
     2641 gctatatgct ggtggaaggt cacggaaact ttggctccgt tgacggggat tcgccggcgg
     2701 ccatgaggta cacagaggca aggatgtcga ggatctctat ggagcttctg gcggatataa
     2761 acaaggatac cgtcgatttt acacccaatt ttgatgagac cttaaaagaa cccaacgtcc
     2821 ttccgtccag gttccccaat cttttagtca gcggatcatc gggtatcgcc gtaggtatgg
     2881 ctaccaatat tccgccccat aacctggggg aaaccataga tggggtagta gctttaatcg
     2941 atgaccctga tatttccatt gaggaaatta tgaaccatat aaagggaccg gactttccca
     3001 caggggcatt aattctcggt agggaaggta tccatcaggc ctacggaacc gggaggggca
     3061 ggataattac cagggcaaag acccagatag aggagtattc aggcaaccgg accagaatca
     3121 tcgtagagga gctgccctat caggtaaaca aggctaggct catcgaaaag atggccgatc
     3181 tggtacggga taagaggata gatggaatat ccgatatccg ggacgaatct gaccgaagcg
     3241 ggatgcggat agcggtagat ttaaagaggg atgccaatcc aaacgtggtt ttaaactacc
     3301 tgtttaaaca tacacagatg caggtgacat ttggggctat aatgttggcc ttagttgacg
     3361 gacaacccag gatcatgaat ttaaaggaga tcctctacca ctatctaaac catcaaaagg
     3421 aaattgtagt aaggagaacc aggtatgaac tggagagggc ggaggccaga gctcacatat
     3481 tagaggggct tttaattgct ctggatcata tcgacgaggt gataagcctg ataaggggct
     3541 cacgtaccga ccaaatagcc aaggaaggtc tgatgcaaaa gtttgggctc agcgaaagac
     3601 aggcccaggc tatattggat atgagactga ggcgcctgac agggctggag agggacaggt
     3661 tggtagagga atacgaaggc cttttaaaaa ccatccgggg cctaaaggac atattgggca
     3721 gtgaagatct ccagtattcg ataatcaagg gagaactcct cagcgtaaag gacagatact
     3781 ccgacgacag aaggacccag attatctctg cacccggtga gatagatata gaggatctca
     3841 tcgatgaaca tgacgtggtg cttaccctca cccatttagg ctatataaag agaacccccc
     3901 tagacgccta tagaagtcag aggaggggcg ggaggggtat taccgggctt cagacaaggg
     3961 aggaggactt tgtcttcagc ctgttcgtta cctccaccca tcagcgtctg ttattcttca
     4021 ccagcttggg taaggtgtat gagctaaagg catatgagat acccgaggcg ggcagacagg
     4081 ctaggggtac ggccattata aatctcttgc agttgatgcc gggtgagagg atacaggccg
     4141 ttatacccgt agatgaattt gaaagcggaa agtttttggt aatcggtact aaaaatgggt
     4201 tggtaaaaaa gagtccccta gaggaatatg aaaatatccg aaggggtggg ctgatcgcag
     4261 ttaacctgag gcaaaatgac gaggtcgtcg gggtccaatt gaccgatggg gacagggaga
     4321 tactactggc aacccatagg ggaatgtgta tcaggtttaa tgaatccgat gtccgttcca
     4381 tgggaagggc ttccatgggg gtcaagggta ttcacttgag cagggatgat tatgtcatag
     4441 atatgatact ggtatccggc caggatgagg tgttggccat aagcgaaaat ggttttggaa
     4501 agcggacttc catagacgaa tacagggttc aaagaagagg cggacgcggt ataattacca
     4561 tgagaaccaa tgaaaagaca ggcaatcttg tggcccttag gatggtatcc gaagatgaag
     4621 acctgatgat tataaattcc gagggcatca tcattcgcat aggggtaaag gagatctcca
     4681 ccctaggccg aaacacccag ggggtaatgc ttatgagagt cgaggaaggg atgaccgtaa
     4741 tatccgtagc gaaggcagaa aaggatgacg atgaagacgc tttttgagtc atccataaat
     4801 tacaggggga ataccgcaat gggaaaaata aaatttataa aaaaagatga actaaaggtt
     4861 aaaccaaaag atagcgaatt ggtatttgga acgcaattta cagattatat gtttgtaatg
     4921 gattatgatg taaaaaaggg ttggtatgat cctaggatag ttccttacgg ccccatagag
     4981 atgagcccat cgtctatggt gtttcactat ggacaggcca tatttgaggg aatgaaggcc
     5041 tatagagaca acaatgggga tatagttacc tttagggcta tggataattt taataggatg
     5101 aaccgatccg ccgacagaat atgtatgccc aaggtagata caggctttgt atgggatgct
     5161 ttgaacaccc tgttggatat cgaacgggac tgggtcccct cctcaaagga tacttccctg
     5221 tatataaggc cgtttatgat tgccaccgat ccgtttttgg gggtacgacc gtctaacacc
     5281 tacaaattct ttattattct gtcgccggtt ggaccctatt atcccgaggg tttagaccct
     5341 gtaaagatat atgtggaaga taagtacgta agggtctcaa aggggggcac cggcgaagcc
     5401 aaggtagcag gaaactatgc tgcaagcctg ctggcagcgg agaaagccaa ggataagggg
     5461 tattcccagg ttctctggct ggacgcagca gatcatacct ttgtagaaga ggtaggcgct
     5521 atgaatatct tctttaagat agacggaaaa ctgataaccg cacccctaga cggcagtata
     5581 ttgcccggga taaccagaga ttcggtcatt aaactggcaa aacatttggg atatactgta
     5641 gaggagcgag acctgagagt ggaggatgta ttcgcggccc atgacaacgg gaccctagaa
     5701 gaggtttttg gttcgggaac agccgccgta atatcaccgg tggggatttt ggaatggatg
     5761 gataacaaaa tagaaataaa cgacggtaaa ataggcgagt ttacctctag gatgtatgac
     5821 atgcttacag gcatccaata cgggacacaa gaggatgtat tcggttgggt caatagggtt
     5881 aagtgtagat aaacgactcc aactacaaaa ttagtaccca tagataaatt cgttttccct
     5941 attgtttatc tataggtgcg atgcaagggg gggtgttgtg tccctccctt gcatccttta
     6001 aagtaacgcc agttcctacg gggtctggct attttgttct atttcaccga tccgattttc
     6061 cagttgaaat gccatctgct gatcaatttg gcctttggca gattgattga tgtgggcacc
     6121 ttggacatca taccaaccat ggttatgcat tacttccata aagagttttg tatggttttg
     6181 ctcttcctgc tgaatattct gaagtgcggt ggttacctct tgatttacgg aatccataac
     6241 ccccgtatcg taggcagatg atacgtacct ttccgtcata atagaatcaa ggagcatttc
     6301 cttttccgat aattggtttt ttgccattta ctatttcccc ctcctttatg atgtgggtga
     6361 cggggggctg aatccccctt gttccagcaa tgatttcagc gtctcggcat gttgtacctc
     6421 ctgttggtac accaggttga atatattttt gatctgggga tccgtggctt gctcagaata
     6481 tagcttgtac ttgctcatac agatgtattc ggcttttaat tgatcctcaa ggatcttctt
     6541 ctccttatcc gataactcca tttctctcct cctagtaata gttctatgga accgggcttc
     6601 tggcatataa tttctaattt ccaacttcca aacaataaag tcatccattt ttcaatagcc
     6661 tattagctct taaataagag ctttctacaa aattatattc tcccaaaaaa agaatcctat
     6721 gcttgtcttg cacaggagga tggtttattc cggcatcgtc caaactacat cgggaagctg
     6781 ccttatatgt cccatctttg ggggattatt tgcggccctt cccttagatg tctttctttt
     6841 aggccgttgg aatctgcctc cacaaatcca tccaccctat taacagtttt gtggaaccca
     6901 acttctaatt tccaactttc aatgcccgtc ttgcatagga ttgcatagtg ttatgttaga
     6961 gttttgcgta ctcgttgatg agttctaaca tcctttcaac gcccttggta tgatccatcc
     7021 tggccatagt atcaatgtac ctatgcctgt tggcgtagac gtctagaatt gccgtaagca
     7081 gtatatcttc ggtcaaggta tcctgatcca gggctttact gaaaccctgt ctttcaaagg
     7141 actcggcgtt ttggatttgg tcccctctgc tggcgctcag ggggagggga atcaatatgt
     7201 tcggcttatt gagggccaaa aattcatata tggaattggc cccggctctt gatattataa
     7261 cgccggctag ggccataaga tgcgggagat cctcatccac atattcaaat tgcttatacc
     7321 ccgggatatt ttccatggtg gggtctaaat tattattacc gcaaagatgt atgacgttaa
     7381 aacggttggt gagcctgccc aacaggctac gaacgaattg gtttatagcc accgcgccca
     7441 ggcttccgcc cataaccatc agggccggct tttctgtggt aaatccgcaa atatccatac
     7501 ccttttttgg atcgcccttt aaaaggccgg ggcgcagcgg ggtgcccgtg tatacgccct
     7561 tgtccccttt aaaaaattcc gtagtctcct taaaggttgt gcagatcttt acagcgaagg
     7621 ggaaggaaat tttattggca aggccggggg tgatatcgga ttcgtggatt atcaccggca
     7681 ccttatttat ccagcccccc agcaccacag gaaccgaaac aaagccgcct ttggaaaaga
     7741 tgatggcggg ttttagcctt cgaattaagt ttatggattg ccctaccccc tgaattaccc
     7801 tgaaggggtc ggtaaaattt tggatatcca tataccttct gagctttcca gaatttatga
     7861 catggtaggt aacgcctttt tccttagtta taaggccccg ttcaatgcca ttttccgtac
     7921 ctatatagtg aatatcgtag ccctgtttta aaagctccgg tatcagggcg atatttgggg
     7981 taacatggcc cgcagtcccg ccgccggtta agactatcgt cttcaaagtt atcaatcctc
     8041 cagcatccga tttcgaatcc cttcacccga catcaggcta taccctttcg ggtgcgctta
     8101 gcccaattag atttaatcca ttggccaagg tgtgatttac tgccagaacc agggccagtc
     8161 ttgcattcct tagatcatca tcttctatca atatggaatg ttcatgatag aagcggttaa
     8221 aatcctgggc cagatctacc aagtatctgg tgattatgga gggctctaga tcattcatag
     8281 cagaaacgac aatatcgggg aagaagtaaa gggttttaat tacccgaaat tcctccctgc
     8341 tcaaaagaag tccggcatca aagttgggat ctacttccat ttttgccctt tttaaaaggc
     8401 tgcatgtcct ggcatgggtg tattgaacgt agggccctgt ttcgccgtca aagtttagga
     8461 tttcgtccca ggaaaaggat acgtccttaa tcctgttgct gctgagatcg ctaaatacca
     8521 ctgcgcccac gcccatttgc cgggcgatct cattcttttc atcttcatcc atattgggat
     8581 tcttctcacc tatgatcctt gaggttttct ttatggcctc ggagaggatg tcctctagga
     8641 ggaccacctt cccttttcgg gtagccagtt tttcaccacc cagactcact ataccgaagg
     8701 ggacgtgttc taatttatcg taccaatcat agcccatcag ctcaataacc ttgaaccact
     8761 gatcaaagtg caggctctgg gcagccccgg ttacatatat acatttttca aaatcatagg
     8821 tatt
//
LOCUS       DASRNX010000024        18079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_746760,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000024 DASRNX010000000
VERSION     DASRNX010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 18079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18079
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746760"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /gene="glgB"
                     /locus_tag="VFD89_00865"
     CDS             <1..1144
                     /gene="glgB"
                     /locus_tag="VFD89_00865"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HZJ56776.1"
                     /translation="FEGGDHQEWGTYEFDFSRLEVWSFLIGNAVFWLDKFHADGLRVD
                     GVTSMLFLDYGKGDSPWKPNIHGGRENLEAIEFLQTLNEVVHELYPGSLMIAEESTDW
                     ASVTTPPEDGGLGFDYKWNMGWMNDTLNYMKTDFSGRMDHHNKLTFSLYYAFSENFIL
                     PFSHDEVVHGKKSLIGRMPGDYWRQFAGLRALRLYQMCHPGKKLLFMGSEFGQFIEWR
                     YYEELEWFLLEYDTHRRYKNFVRDLNILYLKEQSLWENDTDWDGFQWIDVKNAEQSII
                     IFLRKAGDGDFILIVLNFKPDFYPIYRVGVPRSGVYEEIFNSDSRCHGGSGQVNDGPI
                     KAEAVPFHGQDHSIHIKVPPLGGVLIKLSNKYAVLEAENDKEQGKL"
     gene            1119..3551
                     /locus_tag="VFD89_00870"
     CDS             1119..3551
                     /locus_tag="VFD89_00870"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HZJ56777.1"
                     /translation="MIRNKESFKKAYGMKFAETQGKSLEEGSSWDKYQALVSLINSHL
                     APKWVESNRRYYEKREKQVYYFSMEFLIGKLLPHYLSNLGIKDIVREGLVDMGIDLDD
                     LIDAERDAGLGNGGLGRLAACFLDSMASMGIPGHGNGIRYKYGLFKQKIVNGFQAEVP
                     DSWLDNGYPWEIRKPEKAVVVKFKGNVRADNPDGSLTFYHENYEPVLAVPYDIPIVGY
                     RNGGSINNLRLWSAEPMADEFDIASFNRGEYRQAVGYMSEIEAISYILYPDDSTHAGR
                     ELRLKQEYFFVAAGLGSIVRSFKEKMGSLKDFSKKVAIHINDTHPALCIPELMRILID
                     EEGMGWDDAFNTAVETISYTNHTIMPEALEKWPVDIFKYLLPRIYMIVEEIDKRYRIE
                     MARRYPDSMNMIHDTAIIRDDEVWMANLAVIGSHSVNGVSRLHSHILKTHLLRDFHKI
                     YPRRINNKTNGISHRRFLLEANPSLSKLITEAIGEDWFFDPRKLERLIEFREDRVFID
                     KLAESKYENKAALANIIQDRMGISIDPNSIFDIQVKRIHAYKRQLLNIFRIMDIYNQL
                     KVMPNLPYRPHTFIFAGKAAPGYHYAKTIIKLINALANKINNDPDTGDIIKVVFLENF
                     NVTLAERIYPAADISQQISTASREASGTGNMKFMMNGALTLGTHDGANVEIREAVGDD
                     NIFMFGLKANEVMDFYGRRSYDPWQEYHSCYKLKKVIDQLINGFFSEDEGDFRDIYHS
                     LLGGNDEFFVLKDFCSYMEAWYGLNMLYGDPAEWNAIALANIAKSGVFSSDRTIKEYA
                     GDIWHVPVGTVD"
     gene            3798..4937
                     /locus_tag="VFD89_00875"
     CDS             3798..4937
                     /locus_tag="VFD89_00875"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZJ56778.1"
                     /translation="MRKKNIVAMLLAGGQGSRLGGLTREIAKPAVSFGGKYRIIDFSL
                     SNCVNSGIDTVGILTQYKPMLLNSYIGIGTAWDLDFPQGGVHVLPPYMEEDGGNWYRG
                     TGNAIYHNMDFIDSYNPEYVLILSGDHIYKMDYSKMLKFHRERESELTIAVIEVPWEE
                     ASRFGIITADKGDRVIKFSEKPENPDSNLASMGIYIFNWAVLKEALIEDEESSQSHND
                     FGKDIIPMLYNQGKSIFTYKFQGYWKDVGTIESYYETNMDLLGKNPGLDIYDRDFRVF
                     SNLNVLAPQYIGPNAKVINSLISNDCTVLGRVKNSILAPGVFIGENATVKDSIVLPGA
                     RIAKNAFVTRSIIGENYRVLPGSVVGDRGNRRDLPSRITLLADAY"
     gene            5150..6316
                     /gene="glgD"
                     /locus_tag="VFD89_00880"
     CDS             5150..6316
                     /gene="glgD"
                     /locus_tag="VFD89_00880"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HZJ56779.1"
                     /translation="MKNVTGIISSTCHGDILGSLTGERPISTVPFGGRYRLIDFALSN
                     MVNTGIRTVGIVTPHNYRSVLDHLRSGKDWFLDRKSGGLFILPSGEDKTDNPGSNFLL
                     GDMLNNLDFLRKSREEYIVISSCNIVMNTNYREALNFHVSNGNDITLIYKEERLKEKD
                     CKDILLIEQAEDRRVTAIYRNEKENLYRPGKALLNIAIINRGLLIEILKGYDGHESSN
                     FFEVLGQMADKLRIYALEFRGYTGRIISVNSYFQHSMDLLSPRVRTELFMKRDKIHTK
                     ITDNPPTRYGENAQVTNSLMGSGCNIDGHVENSILFREAIIKKGAMVRNSIIMQKNII
                     GKDVVLENVILDKFVTIKDGTVLRGEKDNPITIDKAAVIGPKEVSHEKYNLRSG"
     gene            6288..7724
                     /gene="glgA"
                     /locus_tag="VFD89_00885"
     CDS             6288..7724
                     /gene="glgA"
                     /locus_tag="VFD89_00885"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HZJ56780.1"
                     /translation="MKNIIFAAGEAAPFAKTGGLGDVVGSLPKAIKELGGDVRVIMPK
                     YGGIPQGFMDGYRHIVDFHVDVGWRSQFCALGEVEYQGIHYYFIDNEYYFKRDCLYGY
                     DDDAERFAFFSRAVLESLPRIEGFKPDIIHCHDWHTALLPMMIREHYGEDPFYQGVKT
                     VLTIHNLRHQGIFPLSILDDLLGITEGSEAANNLEFNGAANYFKGGLVYADSITTVSP
                     TYASEILTPYFGEGLDGILRMQRDKLRGILNGIDYRIYNPLRDPNLFVKYRSSLGKKA
                     DNKAALQSLVGLPEDRDVPLLGLVTRLDSQKGLDLLNHIMDELMDEELQMVVLGTGDR
                     AYEDAFYYFASKYPAKLAVKILFDSSLAHKIYASADMLLMPSQFEPCGLAQMIAMRYN
                     TVPIVRETGGLKDTVVPYNEETGEGNGFSFANYNAHELLFTIRRALKVYREDKAAWKG
                     ITESGRKMDFSWNASARSYMELYEELAE"
     gene            7725..9815
                     /locus_tag="VFD89_00890"
     CDS             7725..9815
                     /locus_tag="VFD89_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HZJ56781.1"
                     /translation="MDKPKVYHNSHRNEYRSPFGAMPTGGTARLRLRLDLGNDKNGPG
                     VRCYLRLWEKNSRERLLPMDRVDFPKDSDSMTQLLGADSSRNNGPVAQFLEMDLSEDG
                     GSMTLFFEARYEVPQEPSLVWYYFKIVLGERVFYYGNNAETLGGEGALYETEPPGYQI
                     TVYSPLNVPRWYKDGIMYQIFVDRFFRGGDFRGGDFTSGDTPEGLMDEHGSLYHLDWY
                     DTPFYIRGKDNSITRWTFFGGNLRGIIEKLDYLKELGITIIYLNPIFKARSNHKYDTG
                     DYMIIDPMFGNEKVFAELISEAEKRGINIILDGVFSHTGAYSIYFNKDGTYPSIGAYQ
                     SKDSPYYSWYKFEDYPDEYESWWGVETMPNVDEMEPSYREFIYGGKNSVIRHWMRLGA
                     KGWRLDVADELPSEFIKEMRIAVKETDDEGILLGEVWEDASNKVSYGELREYLLGGEL
                     DSSTNYPLRRIWLDFILGKIDADLAHRRIMGLYENYSKESFYSAMNLIGSHDRVRILT
                     KLGEAPPEEELCEGVKENYRLSPDARKLGIKRLRLISLMQFFFPGVPCIYYGDEAGLE
                     GYSDPYNRGTYPWGREDGEILAWYKKITALRQQYDVLRDGDFKSFYLDRDVYGFIREN
                     SKDRVVVLINRNPNDPWEGELIPRSIGDEGVPWKAGDAIDLLNSDYILKGPRGCLLRI
                     EPLGAMALNFIECR"
     gene            9913..11571
                     /locus_tag="VFD89_00895"
     CDS             9913..11571
                     /locus_tag="VFD89_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HZJ56782.1"
                     /translation="MSKVVYTAKVVSALQKVFSDEEPKFQPECNVLTALKNETVSFQV
                     AYTSGNSRITYGRVEVESPIVNDIRVRKVVEVPSSLCCHPEVDDNYLRTRPGLYPDLL
                     TDLEDGNIAYVAGHWQSLWVDVRVGEDTPAGTHPIIVKFFDLDGKEVCRGETAITIYD
                     VLLPEQELIHTEWFHGDCLADYYRAEVFSQEHWRILENFVAAAAQRGINMILTPQFTP
                     PLDTAIGGERTTIQLIDVTVKDGEYSFDFTRLKRWVDMCLGAGIKYFEMSHLFTQWGA
                     VAAPKVMGVADSEYRQLFGWDTPAVGGEYTEFLQAYLPKLTEKLREWGIADRTYFHIS
                     DEPNLGQLKTYKAAKESVAEYLKDFIIIDALSDYEFYKVGLVENPISSNDHIHTFLDN
                     GVKGVWSYYCTSQYNKVSNRFMSMPSARNRIYGTQIYKYDIEGILHWGYNFYNSQYSL
                     KKINPYAVTDAGGAFPSGDPFLVYPKDDGTPEESIRMMVLYHAMADHRAMKLLESLMG
                     KEYVMGLIEGDLAEPITFETYPKSDMYLINLRNRINREIAKVLA"
     gene            11589..12608
                     /gene="galE"
                     /locus_tag="VFD89_00900"
     CDS             11589..12608
                     /gene="galE"
                     /locus_tag="VFD89_00900"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZJ56783.1"
                     /translation="MKILVTGGAGYIGSHACVELLNAGYEIVVVDNLSNSKEEALKRV
                     QEITGRKLKFYKEDLLDYEAIDAVFKEQSIDGVIHFAGLKAVGESVSIPLHYYHNNIT
                     GTLNLCRAMQKHGVRNLVFSSSATVYGDPSTVPITEDFPLSATNPYGQTKLMIEQILR
                     DLYNSDDSFNIAILRYFNPIGAHESGKIGEDPNGIPNNLVPFITQVASGRLEELKVFG
                     DDYPTSDGTGVRDYIHVVDLVLGHLKALDKLGENPGVVTYNLGTGRGYSVLEMVDAFQ
                     RATGKTIPYKIAERRPGDIATSYADPAKAVKELGWQATKGLDDMSRDSWRWQYNNPDG
                     YGDAL"
     gene            12639..13553
                     /locus_tag="VFD89_00905"
     CDS             12639..13553
                     /locus_tag="VFD89_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZJ56784.1"
                     /translation="MTGPALVIMAAGMGSRYGGLKQIDPIGPNGEIIMDYSIYDAIKA
                     GFSKVVFIIKEENYDIFRDTIGKRIEDRVETAYAFQSLGDIPQGFEIPQGRERPWGTG
                     HAVLSCRRHIDGPFVVINADDFYGRASFETLYTYLMNLDEGDNHNYAMPGFILKNTLT
                     DHGHVSRGVCEVDDKGFLVGIKEHTKIKKFPDSAMYKEGQEDWKRISQDSIVSMNMWG
                     FTPSIFNELETGFSDFLNNARDLMTAEFFLPNLIGDLLKEGKIRVRVLPSKDRWYGIT
                     YREDKAMVEEAVRQMVDKGVYPYNLLEG"
     gene            13563..14660
                     /locus_tag="VFD89_00910"
     CDS             13563..14660
                     /locus_tag="VFD89_00910"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HZJ56785.1"
                     /translation="MDKGFDIERIAGNFQFKGEFQKAEANISGHINDTYIVYFDKGDD
                     EKHRYILQRINHNIFKEPERLMENIVGITRHMREKIIRAGGDPLRETLNLVPTTEGGY
                     FHRCDDGGYWRGYLFIDGARTYQMVENAEHFYKAGKAFGNFQRLLSDYPAEDLHETIP
                     NFHNTRIRFDAFVEAVEKDTLNRAKDVKEEIEFALKRAEDTPVLVDLLEKGELPLKVT
                     HNDTKFNNVMIDDKTGEGICVIDLDTVMPGLSLYDFGDSIRFGANPAAEDERDLSKVW
                     MDLGLYERFTRGYLETAGDSLTKAELRYMPFSAKLMTYECGIRFLGDHLNGDTYFRIH
                     RPGHNLDRARTQFKLVADMEGKLDDMARIIG"
     gene            14863..15489
                     /locus_tag="VFD89_00915"
     CDS             14863..15489
                     /locus_tag="VFD89_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZJ56786.1"
                     /translation="MSETILRADTRDETARKVRDEGFIPGVMYGTGVQDGMSVKFDEL
                     ELNKVLKSQGMNPRMAIMLGEEEKHVLIKEVQRDPVRGNVIHVDLQAIAVDEVIRTTI
                     PIVFEGREDVESRGLLLEVYLFEIEVSGPANIIPDSITMGVGESEAGDTVTMSDIELD
                     SRISAITPLDEVLAVATIPRMEEEEDDEDVDEDVDIMVDTEEDAPEEE"
     gene            complement(15582..15746)
                     /locus_tag="VFD89_00920"
     CDS             complement(15582..15746)
                     /locus_tag="VFD89_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56787.1"
                     /translation="MSLANGLKKIFRKRDRFAPPNEFRLNQKEKESIIVFFSLVGCIA
                     IIFTLLVALY"
     gene            16190..18019
                     /locus_tag="VFD89_00925"
     CDS             16190..18019
                     /locus_tag="VFD89_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HZJ56788.1"
                     /translation="MKVARKKLGFIILDLFCVNISLVLAFLIQNEGMIPQRYKAYLWV
                     MMLIASVIAIGSFHLFQLYNSLWKYASLGEMFMIGFACLLACGGTYLSFRFLKFRVPF
                     GVYMLYGFMIVGLTGLNRLLYRAVKRHKAIINRLFLKGYRRIMIVGAGEAGAMVIREL
                     QSHPEMGMKPIVAVDDNSSKHNSRIKGVRILGGREAIPTLARDMKVDEIMITIPSATK
                     GELKDIIEICKGTKCKLRTLPNVLELIDGKVSVHNIRDIKIEDLLGREPVKVDLNEIS
                     EYLHDEVVLVTGGGGSIGSELCRQIAKQDPKKLLILDIYENGVYDLQQELGRRYPNLD
                     QRVIIASVRERARMEAIFEEYRPGVVFHAAAHKHVPLMEDNPAEAVKNNVFGTLNTAE
                     CADKYGARRFVLISTDKAVNPTNIMGATKRIAEMIIQALNKNSKTEFVAVRFGNVLGS
                     NGSVIPLFEKQIAEGGPVTVTHPEINRFFMIIPEAVQLVIQAGAMAEGGEIFILDMGQ
                     PVKIADLAEDLIKLSGLEPGIDIDIEYTGLRPGEKLYEELLLEEEGLMATRHEKIFIG
                     KPSFFDLQELKLELETLKFTVSGNGENIFKFVQHLVPNYRRVI"
BASE COUNT         5285 a         3683 c         4740 g         4371 t
ORIGIN      
        1 cttcgagggt ggcgatcacc aggaatgggg gacctatgaa ttcgatttct cccgccttga
       61 ggtttggagc tttctcatag gcaacgccgt gttttggctg gataaattcc atgcagacgg
      121 gctgagggtg gacggcgtca ccagcatgtt gttccttgat tacggcaagg gggacagccc
      181 atggaaaccc aatatccacg ggggcaggga gaacttagag gccatagaat ttctccaaac
      241 tctaaacgaa gtcgtccacg aactctaccc ggggtccctt atgatagcgg aggaatccac
      301 ggattgggca tcggttacaa cgccgccgga agacggcggc ctgggatttg actataagtg
      361 gaacatgggc tggatgaatg ataccttaaa ttatatgaaa acggatttta gcggcagaat
      421 ggatcatcac aataagctga ccttctctct ttattacgcc ttttccgaaa acttcatcct
      481 gcccttctcc catgacgaag tggtccacgg gaaaaaatcc ttgataggca ggatgccggg
      541 ggattattgg cgccagtttg cagggctcag ggccttgcgc ctgtatcaga tgtgccaccc
      601 aggcaaaaag ctgctcttta tgggcagcga atttggccag tttatcgagt ggaggtacta
      661 tgaggagctg gaatggtttc tgctagaata cgatacccat agaaggtata agaattttgt
      721 aagggatctg aatatactgt atcttaaaga gcaaagccta tgggagaacg ataccgattg
      781 ggatggattt caatggatag acgtcaaaaa cgcggagcag agcatcataa tcttcctccg
      841 aaaggccgga gatggggatt tcatactgat agttctaaac tttaagccgg acttttatcc
      901 aatatatagg gtaggggtac caagaagtgg tgtatacgag gaaatattca attccgacag
      961 tcgatgccat ggcggatcag gccaggtaaa tgacggcccc atcaaggcgg aagccgtacc
     1021 ctttcatggg caagaccatt ccatccacat aaaggttccg cccctaggcg gcgttctaat
     1081 aaaactatcc aacaaatatg ctgtattgga ggcagaaaat gataaggaac aaggaaagct
     1141 ttaaaaaggc ctacgggatg aagttcgcgg aaacccaggg gaagagcctg gaagagggca
     1201 gctcttggga caaatatcag gccctggtct cccttataaa cagccatctg gccccaaaat
     1261 gggtcgagag caacaggcgc tattatgaga agcgggaaaa gcaggtctac tacttctcta
     1321 tggagttctt aatagggaag ctccttcccc attacctgtc taacttaggg ataaaggaca
     1381 tagttcggga aggccttgtg gatatgggca ttgatctcga tgatctgata gacgcggaaa
     1441 gggatgcagg tcttggaaac ggcggacttg gaaggttggc tgcctgcttt ttagattcca
     1501 tggcatctat gggtatcccg ggacatggaa acggcatacg gtataaatat ggccttttta
     1561 aacaaaagat cgtcaacggg tttcaggcag aggttccgga cagctggctt gataacggtt
     1621 atccctggga aataagaaaa ccggagaaag cggtggtggt aaagtttaag ggaaacgtcc
     1681 gggcggacaa tcccgatggg agccttacct tttatcacga aaactatgag cctgtattgg
     1741 ctgtacccta cgatatcccc atagtggggt ataggaacgg gggcagcata aacaacttgc
     1801 gattgtggag cgccgaaccc atggcggacg aattcgatat agccagcttc aaccggggcg
     1861 aatataggca ggccgtcggg tatatgtcgg aaatagaggc catatcctat atcctctatc
     1921 ctgacgacag cacccatgca ggacgggaac tgaggcttaa gcaggagtac ttctttgtcg
     1981 cggctgggct gggatccata gtccgaagct ttaaggaaaa gatgggttcc ctaaaggatt
     2041 tttctaaaaa agtcgccatt cacatcaacg atacccatcc ggccctttgc atacccgaac
     2101 tcatgcgcat cctcatagac gaagagggca tgggctggga cgatgccttt aatacagcag
     2161 tagaaaccat ttcatacacc aatcacacca tcatgccgga ggccttggaa aaatggcccg
     2221 tagatatatt caaatacctt ctgcccagga tatatatgat agtggaggag atagacaagc
     2281 gttaccgaat agagatggcc agaagatatc ccgactccat gaatatgatc cacgatacag
     2341 cgatcataag ggatgatgag gtttggatgg caaatctggc ggttataggg agccactccg
     2401 taaacggggt atcccgactt cattcccaca tcctaaaaac ccatctactc agggattttc
     2461 ataaaattta tccccgaagg ataaacaaca agaccaacgg aataagccat agaagattct
     2521 tgcttgaagc aaatccaagc ctgtcaaaac tcataaccga ggccatcggg gaggattggt
     2581 tttttgatcc ccgcaaattg gaaaggctga ttgaatttag ggaagacagg gtatttatag
     2641 ataagctggc cgagtcaaaa tatgaaaaca aagcagcttt ggccaatatt atccaagata
     2701 ggatgggcat aagcatcgat cctaattcta tattcgatat acaggtaaag agaatccatg
     2761 catataaaag acaactcctt aatatattta ggattatgga catctacaac cagcttaagg
     2821 ttatgccaaa ccttccctac aggccccata ccttcatctt tgcaggaaaa gcagcaccgg
     2881 gttatcacta tgccaagacc ataataaagc ttataaatgc cttggccaac aagataaaca
     2941 acgatcccga caccggggat attattaaag tggtattttt agaaaacttt aatgtcaccc
     3001 tggccgaaag gatctatccc gccgccgata taagccagca gatatccact gccagcaggg
     3061 aagcgtcggg gaccggcaat atgaagttta tgatgaacgg cgccctaacc ttagggactc
     3121 acgacggcgc caacgttgaa atcagagaag ccgtagggga cgataatata ttcatgtttg
     3181 gtcttaaggc caatgaggtg atggattttt acgggaggag gagctacgat ccctggcagg
     3241 agtatcatag ctgctataag ctcaaaaagg tcatagacca actgataaac ggtttctttt
     3301 cagaggacga gggggatttt agggacatct accattctct tttgggcgga aacgatgaat
     3361 tcttcgtcct caaggatttt tgttcctata tggaagcctg gtatgggctc aatatgctat
     3421 acggagatcc cgctgaatgg aatgctatag cccttgcaaa catcgcaaaa tcaggggtgt
     3481 tctccagcga cagaaccatt aaagaatatg ccggggatat ttggcatgtg cccgtaggta
     3541 ctgttgatta aacctataag cgcggccgga gatcctggcc gtatgcactg cttttcgaca
     3601 ccgctttggg aaggatacaa aggacaccat ggcatgacag tttttataga agaaccatac
     3661 aatggactgg acagcctgac agcctaaaaa aagaaacaac gcaaagacac cggagtctgc
     3721 cggctctaaa aaaacgacac aaaaagacac tgcagctcga cagtttttaa aaatatggat
     3781 gagggtaggg tgatagggtg agaaagaaaa acattgttgc catgttactg gccgggggac
     3841 agggatcaag gctgggcggc cttaccaggg agatcgcaaa gccggcggtt tcctttggag
     3901 gcaaataccg gatcatagac tttagcctaa gcaattgtgt caattccggt attgacacgg
     3961 tggggattct tacccaatac aagcctatgc tgctaaactc ctatataggg attgggaccg
     4021 cctgggatct ggattttccc caaggcgggg ttcatgtact tccaccctat atggaggagg
     4081 acggaggtaa ttggtacagg ggaacgggca acgccatata ccacaacatg gactttatag
     4141 acagctacaa tccggaatac gtgttgatcc tctcggggga tcatatctac aagatggact
     4201 attccaagat gcttaaattc catagggaac gagaatccga gcttaccata gcggtcatcg
     4261 aagtcccatg ggaggaagcg tcccggttcg ggatcatcac cgccgataaa ggggacaggg
     4321 tgataaaatt cagcgagaaa cccgagaacc cggacagcaa tctggcctct atgggcatct
     4381 acatcttcaa ctgggcggta ttgaaggaag ccctgataga ggacgaggag agcagccaat
     4441 cccataacga ttttggcaag gatatcatac ccatgctata caatcaggga aagagcatat
     4501 ttacttacaa atttcaggga tattggaagg atgtaggcac catagagagc tattatgaga
     4561 ccaatatgga tcttttaggg aaaaatccgg gacttgatat ctatgacagg gattttaggg
     4621 tattctccaa tctaaacgtc ttggctcccc aatacatagg gcccaatgcc aaggtcataa
     4681 acagccttat aagcaacgat tgtaccgtat tgggaagggt gaaaaactcc atactggctc
     4741 ccggggtatt tatcggagaa aatgccacgg taaaggattc catagtactc cccggcgcca
     4801 gaatagccaa aaatgccttt gtaaccagat ccataattgg ggagaattat cgggtcctgc
     4861 cgggcagcgt agtgggggac agggggaacc gcagggatct gccgtccagg attaccctgc
     4921 tggcggatgc ctattgaggg cccattacgg catctattta ggttgcgaat cgtctttagg
     4981 gccactttga tattgatatt gatttttagt taaacaatac gaaaactaaa tttatcggca
     5041 taataattac agcggttctg tgcgatttcg tgatgataaa tcttataatt ggggttgtct
     5101 atctttaggt tacggatcgt catattatta aatattagga gggagagacg tgaaaaatgt
     5161 aacgggcatt atttccagca cctgccatgg agatatattg ggaagcctca ccggggagag
     5221 gcccatatcc acggtgccct ttgggggcag gtacagactc atagattttg ccctgtccaa
     5281 tatggtcaat accggcatac gcactgtagg gatagtaacc ccgcacaact ataggtccgt
     5341 attggatcac ctccggtcgg gtaaggattg gtttttggat cgaaagagcg gcggactatt
     5401 tatattgccc agcggcgagg ataaaacgga taatccgggc tctaatttcc tgctgggaga
     5461 tatgctgaac aacttagatt ttctaagaaa gtccagggaa gagtatatag tcatcagctc
     5521 atgtaacatc gtgatgaata caaattatcg ggaagcttta aatttccatg tgagcaacgg
     5581 caacgatata acccttatat ataaagaaga gcggctaaag gaaaaggact gcaaggacat
     5641 attgctcata gaacaggcgg aggacagaag ggttacggcg atttatcgca acgagaagga
     5701 aaacctctac aggccgggca aagcgctgct taatatagcc ataataaaca gggggctctt
     5761 aatcgaaatc cttaagggat acgacgggca cgagagctcc aatttctttg aggtcttggg
     5821 ccagatggcg gataagctga gaatctatgc cctggaattt aggggctata caggcaggat
     5881 tatttccgta aatagttatt ttcaacatag catggatctt ttaagtccta gggttcggac
     5941 cgaactattt atgaagaggg ataaaataca taccaagatc accgataatc cgccgacgag
     6001 atatggggaa aatgcacagg tgaccaattc gcttatggga agcggctgca atatagacgg
     6061 acatgtagaa aatagcatcc tgtttaggga ggccataatc aaaaaagggg ccatggtcag
     6121 aaacagcata atcatgcaga agaacataat cggaaaggac gttgttctgg agaatgtgat
     6181 tttagacaag tttgttacca taaaggacgg taccgtatta aggggggaaa aggataaccc
     6241 cataacaatc gataaagcgg ccgtcattgg gccgaaggag gtatcccatg aaaaatataa
     6301 tcttcgcagc gggtgaggca gcgccctttg cgaaaacagg cgggttgggc gacgtagtcg
     6361 gctctctacc gaaggcgata aaggaacttg gcggggatgt gcgggtaata atgcccaaat
     6421 acggcggcat cccccagggg tttatggacg gatacaggca tattgtggac tttcatgtgg
     6481 atgtgggctg gagatcccaa ttctgcgcct tgggtgaggt ggagtatcag gggattcatt
     6541 actatttcat agacaacgaa tattatttta agagggattg tctttacgga tatgacgacg
     6601 atgcagagag gtttgccttc tttagcaggg cggttttaga gagcctgcca aggattgaag
     6661 ggtttaagcc cgatataatt cactgtcatg attggcatac ggcgcttttg cccatgatga
     6721 taagggagca ctatggggag gaccccttct atcagggggt taagacggtg ttgaccatac
     6781 ataatctcag acatcagggg atattcccgt tgtccatatt agacgatttg ttgggaataa
     6841 cagagggaag tgaggcggcg aacaatttgg agtttaacgg tgcagccaac tattttaagg
     6901 ggggcctggt atacgcagac agcattacca ccgtcagccc cacctacgca tcggagatcc
     6961 tcacccccta ttttggagag ggtcttgacg gcatcttgag gatgcagagg gataagctaa
     7021 ggggcatatt aaacgggata gactatagga tatataaccc ccttagggat ccgaacttat
     7081 ttgtaaagta ccgcagctct ttgggcaaaa aagccgataa taaggcagcc ctccagtctc
     7141 tggtaggcct gcccgaggat agggacgtcc cgctgttggg attggtgacc aggttggaca
     7201 gccaaaaggg cctggacctg ctcaaccaca taatggacga gctcatggac gaggagctgc
     7261 agatggtggt cttgggtacc ggagacaggg cctatgagga cgccttttat tatttcgcgt
     7321 ccaaatatcc agccaagctg gcggtcaaga tattatttga cagttccctg gctcacaaga
     7381 tctatgcctc ggcggatatg ctcctgatgc cctcgcagtt tgaaccctgc ggtctggccc
     7441 aaatgatagc tatgcgctac aacaccgtgc ccatagtcag ggagacgggg gggcttaagg
     7501 ataccgttgt cccctataac gaggaaaccg gggaaggaaa cggctttagc ttcgctaact
     7561 ataatgccca cgaactgctc ttcaccatcc gaagggctct caaggtatat agggaagata
     7621 aggcagcctg gaaggggata acagagagcg gaaggaaaat ggactttagc tggaacgctt
     7681 ccgcccgcag ctatatggag ctctatgagg agttggccga atagatggac aagccgaagg
     7741 tctaccacaa ttcccaccgg aacgaatata gaagcccctt tggggctatg cccactgggg
     7801 gcaccgccag gctcaggtta aggctggatt taggcaacga taaaaatggt cccggtgtaa
     7861 ggtgctatct gcgcctttgg gagaagaaca gccgggaaag actcttaccc atggacaggg
     7921 tggattttcc caaagatagc gattcaatga cccaattatt gggggcggat tcatcgagaa
     7981 ataatggtcc ggtagcccaa tttttagaga tggatttgtc tgaagatggc ggttcaatga
     8041 ccctgttttt tgaagcccga tacgaggtgc cccaagaacc cagccttgtt tggtactatt
     8101 tcaagatagt tttgggagaa agggtattct actacggcaa taatgcagag accctagggg
     8161 gagaaggtgc cctatatgag acggagccac cagggtatca gataacggtg tacagccccc
     8221 taaatgtccc ccggtggtac aaggacggga taatgtacca gatctttgtc gaccgctttt
     8281 ttaggggtgg ggattttaga ggtggggatt ttacgagcgg ggataccccc gaaggattaa
     8341 tggatgaaca cggctccctc tatcatttgg attggtacga tactccattt tatataaggg
     8401 gtaaggacaa cagcataacc cgttggacct tttttggggg caacctaagg ggcataatag
     8461 aaaaactaga ttacctaaag gagcttggca taaccataat ctacttaaac cccatattca
     8521 aggcccgaag caaccataaa tatgacaccg gggactatat gatcatagat cccatgtttg
     8581 gaaacgaaaa ggttttcgcg gagctaatat ctgaagccga gaagcggggg ataaacataa
     8641 tcctggacgg cgtcttcagc catacggggg cctatagcat atactttaac aaagacggca
     8701 cctacccaag cattggggca tatcaatcca aggattcccc ctattatagc tggtataagt
     8761 ttgaagatta tccagatgag tacgaatcct ggtggggggt tgagaccatg cccaacgtgg
     8821 atgaaatgga gccttcatat cgggaattca tctacggcgg aaaaaacagc gtcataaggc
     8881 attggatgcg actgggagca aagggctgga ggttggatgt ggccgacgaa cttcccagtg
     8941 agtttataaa agagatgcgt atcgctgtaa aggagacgga tgatgagggg atactcctag
     9001 gcgaggtttg ggaggatgca tcaaacaagg tcagctacgg ggaactgcga gaatacctat
     9061 tgggaggtga actggattct tccaccaact atcccctaag gcgcatatgg ttggatttca
     9121 tcttaggaaa gattgacgca gacctagccc acagaaggat catgggccta tatgaaaact
     9181 attctaaaga gagcttctat tctgccatga acctgatcgg cagccatgat agggttagga
     9241 tccttacgaa gctgggtgag gctccccccg aagaggagct ttgcgagggg gtgaaggaaa
     9301 actacaggct gagccccgat gccagaaaac ttgggataaa gaggctcagg cttatttccc
     9361 taatgcaatt tttctttccc ggggttccct gcatatatta tggggacgaa gcgggacttg
     9421 aaggttactc ggatccatat aacaggggga cctatccctg gggcagggag gacggagaaa
     9481 ttctggcctg gtacaaaaag attacagccc ttaggcagca atacgatgtt ttaagggatg
     9541 gggattttaa atccttttat ctggataggg acgtatatgg ctttataaga gaaaattcca
     9601 aagatagggt tgtagttctt ataaacagaa accccaatga tccatgggaa ggtgagttaa
     9661 ttccccgcag tataggggat gaaggtgtcc cgtggaaagc aggggatgcc atcgaccttt
     9721 taaattccga ttatatatta aaagggccta ggggatgtct attaaggata gaacccctag
     9781 gcgccatggc tttaaatttt attgaatgca ggtgacccct tatgctataa ttattttgta
     9841 gatcggactc attatcagcc ggcacaatag gctgtatcag catatgaata ttatagaaat
     9901 gggggagttg gtgtgagcaa ggttgtatac accgcaaagg ttgtaagcgc cctacaaaag
     9961 gtattctccg atgaggagcc caaatttcaa ccggaatgca acgtattaac ggcccttaaa
    10021 aacgagacgg tttcatttca ggtggcctac acatccggta acagtcgcat tacatatgga
    10081 agagttgaag tagaatcgcc catcgtgaac gatatcaggg taagaaaggt agtggaagtc
    10141 ccatcgtctc tctgttgcca tcccgaggtg gacgataact atctcaggac aaggcccggc
    10201 ctctatccgg atcttttgac ggacttagaa gacggaaata tcgcctatgt ggcaggccat
    10261 tggcaaagcc tgtgggttga tgttagggta ggggaggata cgcctgccgg gacacatccc
    10321 atcatcgtta agtttttcga tcttgacggg aaagaggtat gccgcgggga aacagccatc
    10381 accatctatg acgtccttct cccagagcag gagcttattc acacggaatg gttccacggg
    10441 gactgtttgg cggattatta tagggcggag gtcttttcac aggagcattg gcgtatactg
    10501 gaaaactttg tagccgctgc ggcccagcgg ggcatcaata tgatcctaac cccacaattc
    10561 accccacccc tagatacggc cataggcggg gagcgcacaa ccatccaact cattgatgtt
    10621 acagttaaag acggtgaata ttcctttgat tttacaagat taaaacgctg ggtcgatatg
    10681 tgtctggggg cagggatcaa gtactttgag atgtcccacc tgttcaccca gtggggggcc
    10741 gtcgccgcac ccaaggtcat gggggtggcg gatagcgaat atagacagct ctttggatgg
    10801 gacacaccgg ctgtaggcgg ggaatatacc gagtttttac aggcctatct cccaaaactc
    10861 accgaaaagc taagggaatg gggcatcgcg gacaggacct attttcatat ttccgatgaa
    10921 ccaaaccttg ggcagctgaa aacctacaag gctgccaagg aatccgtagc ggagtactta
    10981 aaggatttca tcatcataga cgccctgtcg gattatgaat tctacaaggt gggtctggtg
    11041 gaaaacccca taagctccaa cgatcacata catacatttt tagataacgg agtgaagggc
    11101 gtatggtcct attactgtac cagccaatac aacaaggtga gcaatcgctt tatgtccatg
    11161 ccgtctgcca gaaatcggat ctacggcaca cagatctaca agtacgatat agaggggata
    11221 ctgcattggg gctataactt ctataactcc cagtactctt taaaaaagat aaacccctat
    11281 gcggttaccg atgccggcgg agccttccca tcaggggatc cgtttttggt gtatccaaaa
    11341 gatgacggta ccccggaaga atccatacgg atgatggtcc tctaccatgc aatggcggac
    11401 caccgggcca tgaaacttct tgaaagcctt atgggcaagg aatacgttat gggactcata
    11461 gaaggggatt tggcagagcc aatcaccttt gagacctatc caaaatccga tatgtatctg
    11521 attaatttgc gtaataggat aaacagggag attgcaaagg tgctggccta atatatggag
    11581 gtgaaaagat gaaaattttg gttacaggtg gggcgggtta tataggaagc catgcctgcg
    11641 tggagctttt aaatgcggga tatgagatag tggtggtgga taacctaagt aacagcaagg
    11701 aagaggccct aaagagggtg caggagatca ccggcaggaa attgaaattc tacaaagagg
    11761 atcttctaga ttatgaggcc atagatgctg tctttaagga gcagtccata gacggcgtga
    11821 tacactttgc cgggctgaag gccgtaggcg aatccgtatc aatacccttg cactattatc
    11881 ataacaacat aacgggaact ttgaaccttt gcagggccat gcaaaagcat ggggtcagga
    11941 atctggtctt tagctcctcg gctacggtgt atggggatcc cagtacagtg cctataacgg
    12001 aggactttcc cctatcggct accaaccctt acggacagac aaagctgatg atagaacaaa
    12061 tcctgcggga cctgtataat tcggatgata gctttaacat cgcaatccta aggtacttta
    12121 acccaatagg ggctcatgaa agcggcaaaa ttggagaaga tcccaacggg attcccaaca
    12181 acttggtacc cttcattacc caggtggcat cgggcaggtt agaggagctt aaggtgtttg
    12241 gagacgatta tcccacctct gacgggacgg gggttcgaga ctatatccat gtggttgatc
    12301 tggtgcttgg gcatttaaag gccctggata agctcgggga aaatccggga gtggtaacct
    12361 ataacctggg tacaggccgg ggctacagcg ttttagagat ggtcgatgcc tttcagcggg
    12421 caacgggaaa aacgatcccc tacaagatag cagaaagaag gccgggggat atcgccacca
    12481 gctatgccga tcctgcaaag gccgtaaaag agctgggttg gcaggcgacc aagggactcg
    12541 atgatatgtc cagagattca tggaggtggc aatacaacaa tcccgacggc tatggggacg
    12601 cgctttaata tttaataggg gatggaggag gatcacagat gacaggacct gcactggtta
    12661 ttatggcagc gggaatggga agcaggtatg ggggattgaa gcagatagat cccatcggtc
    12721 caaacggtga gattatcatg gactattcca tatacgatgc cataaaggcg ggatttagca
    12781 aggtagtatt tataataaag gaagagaact atgatatttt tagagatacc ataggcaaga
    12841 ggattgagga cagggtagaa acggcctatg cctttcaatc cctgggcgat attccccaag
    12901 ggtttgaaat accccaaggc agggagagac cctggggaac gggccatgcg gtgctctctt
    12961 gcagaaggca tattgatggg ccctttgtgg ttataaacgc agacgacttt tatggcaggg
    13021 catcctttga gaccctttac acctacctca tgaaccttga cgagggtgac aatcacaatt
    13081 atgctatgcc gggtttcatc cttaaaaaca ccctgacaga tcacggccac gtgtcaaggg
    13141 gtgtatgcga ggtggatgac aagggctttt tagtggggat aaaagagcat accaagataa
    13201 agaagtttcc tgattcggca atgtataagg aaggtcagga ggattggaaa aggatcagcc
    13261 aagacagcat agtttccatg aatatgtggg gctttacccc cagtattttt aatgaactgg
    13321 agacggggtt ttctgacttt ttaaacaatg cgagggattt aatgacagcc gaatttttcc
    13381 ttccaaacct aatcggggat ctgctaaagg aaggcaagat aagggtaagg gtcctacctt
    13441 caaaggatcg ttggtacggc atcacctata gggaagataa agccatggta gaagaggctg
    13501 taaggcagat ggtagataaa ggggtatacc cctataattt attggagggc tgagtcatgg
    13561 gaatggataa gggttttgat atagaaagga tcgccggaaa cttccagttt aagggcgagt
    13621 ttcaaaaggc cgaggcaaat atatccggcc acataaacga tacatacata gtctattttg
    13681 ataagggcga tgacgagaaa catcgataca tccttcaaag aataaaccac aatatcttta
    13741 aagaacccga aaggctcatg gaaaacattg tagggataac ccggcatatg agggaaaaaa
    13801 taattagggc agggggagac cccttacggg aaaccttgaa cctggttccc acaaccgaag
    13861 gcggatattt tcacaggtgc gatgacgggg gttattggag aggttatctg tttatagacg
    13921 gggccaggac ctaccagatg gtagaaaacg cggaacattt ttacaaggcg ggcaaggcct
    13981 ttggtaactt tcaaaggctt ttaagcgatt atcccgcaga ggatcttcat gaaaccatcc
    14041 caaactttca taatactaga attaggtttg atgcctttgt agaggccgtg gagaaggata
    14101 ccttaaacag ggccaaggac gtaaaagagg agatagagtt tgccctaaag agggcggagg
    14161 acacccctgt attggtagac cttttggaaa agggagagct gcccttaaag gtaactcata
    14221 acgataccaa gtttaataat gtaatgatcg atgataagac gggggagggg atatgcgtta
    14281 tcgatttaga taccgtgatg cccggcctat ccctctatga ctttggagat tccatacgct
    14341 ttggggcgaa ccccgccgcc gaggatgaga gggatctgtc caaggtatgg atggaccttg
    14401 ggctctatga gcgatttacc aggggctatt tagagaccgc cggagacagc ctcacgaagg
    14461 cggagcttcg gtatatgcca ttttccgcaa agcttatgac ctatgagtgc gggatcagat
    14521 tcctaggcga tcacttaaac ggggatacct attttaggat ccacagaccg ggccataacc
    14581 tagatcgcgc ccgcacccag tttaagctgg tagcggatat ggaaggtaag ctggatgata
    14641 tggcaaggat cataggatag ggatacaccc caccgggctt tctatatgtt ttacgacaaa
    14701 cattctacat tggtagacaa gtgtcgtaaa acattatcct taattcgtaa catatctagc
    14761 ctatatttga ctttaatgaa aaaagtctat attatttgca taggacaata acattttttt
    14821 aaaatatttg gttgagataa ccgtctaatt ggaggtaata gtatgtcaga aaccatcctt
    14881 agagctgaca ccagagatga aactgctcgt aaggtaaggg atgaaggatt tattcccggg
    14941 gttatgtatg ggacaggcgt tcaagatggc atgtccgtta agtttgatga actggaactt
    15001 aacaaggtct taaagagtca gggcatgaac cccaggatgg ctataatgct gggtgaagaa
    15061 gaaaaacatg ttcttattaa agaggtccaa agggaccctg taaggggaaa tgttattcac
    15121 gttgaccttc aggccatagc cgtcgacgag gttataagga ccactatccc aattgtcttt
    15181 gaaggcaggg aagacgtaga gagcaggggg ctgttactcg aggtttacct ctttgaaatt
    15241 gaggtctccg gtcccgctaa tataattccc gattcaataa ccatgggagt tggcgaaagc
    15301 gaggcagggg atacagtaac catgtcggat atagagctcg attctcgcat ttccgccatc
    15361 acacccctag atgaagtatt ggccgttgcg acaataccta ggatggaaga ggaagaagac
    15421 gacgaagatg tggatgaaga tgtggatatc atggtagata cggaagaaga tgcaccggaa
    15481 gaagaataaa aacaaaaaag gccctggaac acggcaacac gccccagggt cttttctatg
    15541 gtcattttta tttaagactt ttgtacacga actaataaaa tctaatacaa cgcgaccaat
    15601 aatgtaaaga taatcgctat acaacccacc aagctaaaga aaactattat gctttccttt
    15661 tccttttgat tgaggcgaaa ttcattgggt ggtgcgaacc tatcccgttt cctaaatatc
    15721 ttcttaagtc catttgcgag agacatatac agcgactcct ttataaatct tatagaataa
    15781 tcaaatgcaa attttattac atatactatt attatacatt atgggctact ggcactatta
    15841 ttatacattt ttagacatag aggggctata gtatataggg actatatagc tgtaggaccg
    15901 tattatcatg gaatagcccc gctgatagga gcaatttatc accgggcttg caaataaatg
    15961 tcaatgccat gcaaaaatga ttgattttta ggatttaata tgttattatt atttcaacat
    16021 tggttaacaa ttaagtttag ttaattgtta gttatctagg attcctaaag gctgtattgc
    16081 tgtttcggcg cactatagtc tgagggatgt taaacacaca tggatatagt aacggcatag
    16141 tgtcgggaca aggtatacta tacccttttt gcgtatgggg gtgcaagtta tgaaagttgc
    16201 gagaaaaaaa ttaggtttta ttatactgga tctattctgc gtaaatatat ccttagttct
    16261 ggcatttctt atacaaaacg agggaatgat accccaaagg tataaggctt atctatgggt
    16321 gatgatgtta atcgcatcag ttatagccat aggttcgttt catttatttc agctatacaa
    16381 cagcctctgg aagtatgcca gcttaggcga gatgtttatg attggatttg cctgtctatt
    16441 agcctgcggg ggaacgtatc tcagctttag atttcttaag tttagagtac cttttggtgt
    16501 atatatgcta tacggtttta tgatagtggg gcttacgggg cttaacaggc tcttgtatcg
    16561 ggcggttaaa aggcataagg ccataataaa tcgtctattt ttaaagggct atcgtaggat
    16621 catgatagtc ggcgcgggtg aggccggggc tatggtcatc agggaacttc aaagccatcc
    16681 tgagatgggg atgaaaccca tagttgcagt agatgataac tcctcaaagc ataattcacg
    16741 gataaaaggg gttcgaatcc taggcggaag ggaagccata cccactctgg ctagggacat
    16801 gaaagttgac gagattatga ttacaatccc ctcagctacc aagggtgagc tgaaggatat
    16861 aatagagata tgcaagggca ctaagtgcaa attaagaacc ctgccaaacg tgttggaact
    16921 catcgacggt aaggtatcag ttcataatat tagggatatt aagattgagg atttactggg
    16981 cagggaaccg gttaaggtgg acctaaacga gatatccgaa tatcttcatg atgaagtggt
    17041 attagttaca gggggcggcg gatctatagg ctccgagctc tgcagacaga ttgcaaagca
    17101 ggatccaaaa aaactgctga tcctggatat atatgaaaat ggcgtatatg accttcaaca
    17161 agaacttggg agaaggtatc caaacctgga ccaaagggtc ataatcgcat cggttcgaga
    17221 acgggccagg atggaagcaa tatttgaaga atataggccc ggagtggtat ttcatgcggc
    17281 tgcccataag cacgtccctc taatggagga taatccggct gaggcggtga aaaataacgt
    17341 ttttgggact ttaaataccg ctgaatgtgc agataagtat ggggcgagac gttttgtcct
    17401 catctctact gacaaggccg tgaaccccac caacatcatg ggggccacca agcgcattgc
    17461 cgagatgatt atccaggcat tgaataagaa tagtaaaacc gagtttgtgg cagttcgctt
    17521 tggaaatgtt ttaggcagca acggcagtgt aatacccctt ttcgaaaaac agatagcaga
    17581 gggtggaccg gttaccgtga cccatcccga gataaatcgc ttctttatga tcatccccga
    17641 agcggtccaa ttggtcatcc aagcaggggc catggcagaa ggcggggaaa tttttatcct
    17701 agatatggga caacccgtta aaatagctga tctggctgaa gatttaatta agctatccgg
    17761 tttagaacct ggaatagata tcgatataga atatacgggg ctcagacccg gggaaaagct
    17821 gtatgaggaa ctactcctag aggaggaagg gctgatggca actcgtcatg agaagatatt
    17881 tattggcaag ccctcattct tcgatctgca ggaactaaaa ctagaacttg agactctaaa
    17941 gtttacagta tcgggtaacg gagaaaatat atttaagttt gttcagcact tggtaccgaa
    18001 ttatagaaga gttatatagg gatgcctaaa gagcatcctt tttatatgta atgggggcct
    18061 ctattcttat atattatat
//
LOCUS       DASRNX010000025        11367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_4069,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000025 DASRNX010000000
VERSION     DASRNX010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11367
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4069"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..501)
                     /locus_tag="VFD89_00930"
     CDS             complement(82..501)
                     /locus_tag="VFD89_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZJ56789.1"
                     /translation="MAKTKIHGDLAGNMGFKMVLDGHTLITDASEEIGGNNLGPRPKQ
                     LLLAGLIGCTGIDVMSILKKMKVNVDDCSIEVAADNTEEHPMVYENIHLIYRFKGKDL
                     PLKNIEKAVALSQDKYCGVSAMLKKATPITYEIVADE"
     gene            686..1933
                     /locus_tag="VFD89_00935"
     CDS             686..1933
                     /locus_tag="VFD89_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZJ56790.1"
                     /translation="MKPTYKNTLYACYLGFITLAIVNNFAPLLFVIFHDEFGISFEKI
                     GRLILVNFTTQMVVDFLAIRYVDKIGYRMAAVLSHLFSVLGLISLGIFPLIFPSPYMG
                     VAVAVVIYAIGGGLNEVIISPIVESLPGEAKASAMSLLHSFYCWGQLAVVVITTILIK
                     LLGTQIWYILPVFWALIPIYNLFNFIRVPLMPTVSGDEKTPITKLLSSRIFLIAILLM
                     ISAGASELTMSQWSSLFAEKGLGVSKMVGDILGPALFAVFMGIGRVAYGVWGQKIDLK
                     KVLMCSSILCILCYGAAVFIKLPIVSLLGSSLCGLAVSLMWPGTISLSAQMYPMGGTA
                     MFSILALFGDVGASLGPWLAGFVSDLAQRSPGLLAIGKANALNPEQLGLKVGLFTAMI
                     FPIILFVGIKLLRKERLVRNRGS"
     gene            complement(1983..3575)
                     /locus_tag="VFD89_00940"
     CDS             complement(1983..3575)
                     /locus_tag="VFD89_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZJ56791.1"
                     /translation="MSQKPTIGGTNTDRLKDFKKPPLSYWLSSTQSTKYPALDGDIDV
                     DILIIGGGLVGFACAYQLLGLTPSIAILEANTVFGGVTARTTAKITSQHGLIYNKIKT
                     MMGKELAQQYADANQWAIEEIKRISDKHGIQCDYVTEPAFVYTQQNKNLQKIKDEVRA
                     AAGLGISASFADAIPFDMPIKGAVRFDNQARFHPLKFGLGLAQYIAKQGVQIFENSRA
                     VDIEENGSYVVTASQGKRITARKVIIASHYPFYNKHGMYFARIYSERAYALAIKAAEK
                     FPGGMYVNADDPARSLRNQETQDGEIILIVGEHHKTGQGRSTIQHYEALADFANTLFT
                     VQDIPYRWSTQDCMTVDGLPYVGNYTLNTPNLYVATGFQKWGITNSIVSSAILRDLII
                     HGKSSWQDVYNPSRKTIVASSKNFIVENLNVAEQLLDGKLSQLPKSMKLEPGDAQIFR
                     MDGKRIGAYRDRTGNLHLVNTTCTHMGCELNWNSAEESWDCPCHGSRFTYEGKVISGP
                     AVKPLRASDDVSTIGKLFTDDF"
     gene            4066..7590
                     /gene="nifJ"
                     /locus_tag="VFD89_00945"
     CDS             4066..7590
                     /gene="nifJ"
                     /locus_tag="VFD89_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HZJ56792.1"
                     /translation="MYKQETMDGNQAAAYASYAFTEVAAIFPITPSTPMAEGVDEWSA
                     HGKKNIFGREVKVVEMQSEAGAASAMRGALQAGTLGSTYTASQGLLLMIPSLYKMAGE
                     LLPGVLHVSARSIATHALSIYGDHQDVMACRQTGVAMLASGDAQEAMDFGFISHLAAI
                     KSRIPFIHFFDGFRTSHEYQKVRLVDYEDIKKIVDYKAIEDFRNRALNPERPVVRGTT
                     QNPDIYFQQREGANPFYDAVPNIVKGYMDGMEAITGRSYRLYEYYGAQDAENVIVAMA
                     SVTGTISEVVDHLNAGGEKVGMVKVNLYRPFSEEHFHKILPKTIKRIAVLDRTKEPGS
                     IGEPLYLDIVKAFKDVANAPTIVGGRYGLSSKDTRPSQIFAVFENLKQDRPKDGFTIG
                     IVDDVTFKSLPETEIINTEPAGTISCRIWGLGSDGTVGANKTAIKIIGDNTDLYAQGY
                     FSYDSKKSGGTTVSHLRFGPKPIKSTYLVYNADYVACHNQAYVHHYDLLKGLKREGTF
                     VLNCSWNMDELDEHLPADMKRYIAQNAIKFYIIDAMKIASEVGLGNRINMVMQAVFFK
                     LANVLPVDEAIGYLKDSIEDMYGRKGQNIVEMNNRAVDSSLEALVQIEVPAVWGTAED
                     DVTDDSHVPDFVENIQTPMARHQGDELPVSAFVGMEDGTFPMGTTAYEKRGIAPMLPQ
                     WQVERCIQCGRCSYVCPHATIRLFLLDDEEKKRAPETFETKKATGRGIDHLQFRVQVD
                     PLDCTGCANCADVCPAKGKALVMQPAEQEIRMQEGNWEFAMTVSMKDDLTNTNTLKGS
                     QFRRPLLEFNGACPGCGETPYIKLLTQLFGERMVISNATGCSSIWGASAPSIAYTTNW
                     NGKGPAWINPLFEDAAEFGYGMALGAGQIREKLADLMRGAIDSSLPSDIKEAFENWLD
                     SMYYGEESKKASEAVLRVLGDHDPMDNGVVQEILKRKDYLVKRSVWAIGGDGWAYDID
                     YGGLDHVLASGEDINIFVMDTELYSNTGGQSSKSTPAACVAKLAASGKKTAKKDLGLM
                     AMTYGYVYVAQIAMGADMNQTIRAINEAERYRGPSLIIAYSPCISHGIKTGMGTSILQ
                     EKRAVDAGYWHLYRFNPDLRETGENPFVLDSKKPTKDYREHIHGEVRYSQLMNVFPEI
                     ADDMFDLSAQHAAERYKRYLGLAEHEIF"
     gene            complement(7642..7809)
                     /locus_tag="VFD89_00950"
     CDS             complement(7642..7809)
                     /locus_tag="VFD89_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HZJ56793.1"
                     /translation="MSKGRYKIKNALVPIENHYTAAWANAEGIKPDSKVNLPNEIQIR
                     NAKEYVDTNQK"
     gene            8161..8973
                     /locus_tag="VFD89_00955"
     CDS             8161..8973
                     /locus_tag="VFD89_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006210795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HZJ56794.1"
                     /translation="MKNQKPDVAAYLNRIGYDGPLDGSAKALAKIQEGHLHSIPYENF
                     DIMAGIPLSLEVTDLLDKIVYRGRGGYCFELNALYKWLLEELGYPTISFFARFWRDEP
                     NAPPKRRHHVLKVEAQGEYYLCDVGVGGIVPRWPIKMVEGLIHRQGDESYRLELDDYY
                     GWVLCELKDGKWEWLYSFTEEPQLPKDYIMASFWCEKSPDSIFIEDAMVAVRTKEGRN
                     TIAGKEFRIFIGDEVHTFVPKTVEEFTDALIEYFGLDISHISHRIWDVNFER"
     gene            9143..9358
                     /locus_tag="VFD89_00960"
     CDS             9143..9358
                     /locus_tag="VFD89_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HZJ56795.1"
                     /translation="MTTKTYQLETLTCPSCMVKIEGALKRTKGVEKSRVLFNSSKAKV
                     TFDENVVDSATIKEAIETLGYEVLGEK"
     gene            9408..10088
                     /locus_tag="VFD89_00965"
     CDS             9408..10088
                     /locus_tag="VFD89_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZJ56796.1"
                     /translation="MKCNCMKRDGKNCIELVPIFNNLSHDEMMEIAGITGDRIYEKGN
                     MIYMAGDVGESLYVIHRGKVKIYRLSDSGKEQVLRVLGPGEFMGELTLFSHNPHTDNA
                     EALEKTTVCVINGVEIRNLMLKYPDIAFKVLEELSQRLERAENLIENISLHSVERRLA
                     DTLLDMAGDKGEIDLKMSRKDLASHLGMSRETLSRKLSSFQDVGFIQLIGHRKVIILD
                     ADALMEIE"
     gene            10269..10583
                     /locus_tag="VFD89_00970"
     CDS             10269..10583
                     /locus_tag="VFD89_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56797.1"
                     /translation="MNMIDGRGPFFPYPFNLYPDEGDQDGGKVADERYHVYVNGDYVG
                     DRLSIAQGDVGWQAVEGYLKGRDFSGYRVTKDGDQVYVDVQNEEEAEDIRSHLSIYTR
                     IE"
     gene            complement(10680..>11367)
                     /locus_tag="VFD89_00975"
     CDS             complement(10680..>11367)
                     /locus_tag="VFD89_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZJ56798.1"
                     /translation="IKTNYILLKDFFKYGFPVILGDIFWGLNLTVQGIIIGRLGSTAQ
                     ASVSIANIVFSLISVGCYGTSGATSVIIGQTVGAGEYEKVKEYAKKLQLLFLIIGFIS
                     GAILYLIKDYILLLYNISEDTIALSRQLIVVLSVTIIGTSYQMSSLTGIVRAGGAIYF
                     VLINDLIFVWLVVIPSALIAHYVFNAPPAVVFACLKCDQILKCGVAVVKVNRFKWIQN
                     LTRDAVAVSS"
BASE COUNT         3111 a         2396 c         2819 g         3041 t
ORIGIN      
        1 tttatttaca atagatataa ctgggctata aaccgttacg gtttatagcc ctaatttttt
       61 gcaacatggg gaatgccatt cctattcatc tgcgactatc tcataggtga tgggtgtggc
      121 cttttttagc attgcgctca ccccgcaata cttatcctgg gatagggcaa cggctttttc
      181 aatgttcttt agtggcagat ccttaccctt gaacctgtat atcaaatgaa tgttttcata
      241 gaccatggga tgctcctcgg tattgtctgc cgctacctct atgctgcaat catcaacatt
      301 aaccttcatc ttcttcaaaa tggacatgac atcgataccg gtgcacccga ttaatcccgc
      361 caataaaagc tgctttggac gcggcccaag gttgttgccc cctatttctt ccgaggcatc
      421 ggtaatcaac gtatggccat ccaagaccat cttaaaaccc atattgcccg ctaaatcgcc
      481 gtgaatttta gttttagcca tcaatgaaac ccccttaaaa atttatttta gttgttgtaa
      541 taataccccc atagggatta tttaaacaca gcaaggtggt gccatatacg tgataatcat
      601 gatataataa acccattgaa tcggtgtgaa gcaagccaat cgaggcttgc atccaccaat
      661 attaatcagc tttggaggaa gacagatgaa acctacatac aagaacacgc tctatgcctg
      721 ctacctggga tttataacgt tggcaatagt aaacaacttc gcacccctgc tctttgttat
      781 attccatgat gaatttggaa tctcctttga aaaaatcggc aggcttatcc ttgtcaattt
      841 tacaacccaa atggtggtgg actttttggc cataaggtat gtcgacaaaa tagggtatag
      901 gatggcagcc gttttatccc acctgttcag tgtgttggga ctaatttcac tgggtatatt
      961 cccattaata tttccatcgc catatatggg cgtagccgtt gccgtggtta tatatgcaat
     1021 cggtgggggc ctcaatgagg tcatcatcag ccccattgta gaatctctgc cgggggaggc
     1081 caaggcttct gctatgagcc ttttgcattc attttactgc tggggtcaat tggcggtggt
     1141 ggtaatcacc acaatactca taaagctatt ggggacccag atatggtata ttttgcccgt
     1201 attttgggcc ttaatcccca tatacaattt gtttaatttt ataagggttc ccctcatgcc
     1261 aacggtttca ggggacgaaa agacccccat taccaagcta ttgtcgtcgc ggatatttct
     1321 tattgctata ctgctcatga tcagtgcagg cgcatcggag ctgaccatgt cccaatggtc
     1381 atcactgttt gccgaaaagg gcctgggtgt cagtaagatg gttggagata tactggggcc
     1441 ggccctgttt gcggtattta tggggatcgg gagagtcgca tacggggttt ggggacagaa
     1501 gattgattta aaaaaggtgc ttatgtgtag cagcatacta tgtatcctct gctatggggc
     1561 cgccgtgttt attaagctcc ccatcgtatc tctattagga agctctctat gtggcttggc
     1621 agtcagccta atgtggcccg gaaccattag cttatcagcg cagatgtacc ccatgggcgg
     1681 gaccgccatg tttagcattt tggcgttgtt tggggatgta ggtgcatcct tggggccttg
     1741 gctggcgggt tttgtatcgg atttagccca gaggtcgccg ggactcctag ctattggcaa
     1801 ggctaatgcc ctcaacccag agcagctggg gctaaaggtg ggtttattta ctgcaatgat
     1861 attccccatc atattgtttg tgggaataaa gttattaaga aaagagcgat tagtccggaa
     1921 tcgtggatcc tgatagcgga ctaatcatga gtattaatcg gcaaattcat aaattgaatg
     1981 tcttagaaat catcggtaaa taattttcct atggtgctga catcatcgct tgccctcagg
     2041 ggttttaccg caggccccga gataaccttg ccttcatagg taaatctgga accgtgacac
     2101 ggacaatccc aggattcttc cgcagaattc caattcaatt cgcagcccat atgggtacag
     2161 gttgtattga ccagatgcag gttgcccgtt ctgtcccgat aagcccctat ccgtttaccg
     2221 tccatcctga agatttgggc atcacccggc tccagtttca tggattttgg cagctggctg
     2281 agcttcccgt ccagcagctg ttctgctacg ttcaggtttt ccacgataaa gtttttagaa
     2341 gaggcgacga tggttttccg ggagggattg tagacatcct gccaggagct tttgccgtgg
     2401 attatcaaat ccctcaagat tgcagatgat actatgctgt ttgtgattcc ccatttctga
     2461 aatcccgtag ccacatagag gttaggggtg ttgagggtat aattgcccac atacggtagc
     2521 ccgtctacgg tcatacaatc ctgggtagac cagcgatagg ggatatcctg taccgtgaaa
     2581 agggtattgg caaaatccgc aagggcctca tagtgctgaa tcgttgacct gccttgtccc
     2641 gttttgtggt gctctccgac gatcaatata atttcaccgt cttgggtctc ttggtttcgc
     2701 aatgagcgcg ccggatcatc cgcgtttaca tacatacccc cggggaattt ttctgcggct
     2761 tttattgcaa gagcgtaggc cctttcggaa tatatcctgg caaagtacat gccgtgctta
     2821 ttatagaagg ggtaatggct tgcgattatc accttccttg ctgtaatcct ttttccctgg
     2881 gaggcagtga ccacgtagct gccgttttct tcgatatcaa ccgccctgct gttttcaaat
     2941 atttgaactc cctgttttgc aatgtattgg gccaatccaa ggccaaactt tagggggtga
     3001 aaccgggcct gattgtcgaa acgcactgca ccttttatgg gcatatcaaa tggtatcgca
     3061 tctgcaaagg atgcactgat gccaagccct gcagcggctc tgacttcatc ctttatcttt
     3121 tgcaaattct tattttgttg ggtgtagaca aaggcgggtt cggtaacata atcacattgt
     3181 attccgtgct tatctgaaat tcttttaatc tcctctatgg cccattggtt agcatctgcg
     3241 tattgctggg ccaattcttt tcccatcata gttttgatct tgttatagat aagcccgtgt
     3301 tgggaggtta ttttggctgt agtacgggca gttacgcccc caaacacggt gtttgcttcc
     3361 aggatagcaa tgcttggagt caggcccaat agctggtaag cgcaggcgaa gcctacaaga
     3421 ccgccgccga ttatcaatat gtcaacatca atatcaccgt ccagggcggg atatttcgtg
     3481 gattgggttg atgacagcca ataggataaa ggcggcttct taaagtcctt aagccggtcc
     3541 gtgttcgtcc cacctatcgt gggtttttgc gacatacctg aggtaatact cttatcatcc
     3601 aattcaattc tccttaaagt gcatattcct gtttattagc ctgtctatta gatagaaaac
     3661 aatacgaaaa ctaaattcat cggcatgaag attgcatagg gtctgtacaa tttcatggtt
     3721 ataaattcta tagcttgggt tgtttgtcta taatatctgt gattccccat aataatatcc
     3781 aatattccaa acgatggcat ttaaaattat catttcggta cgggcatgat aattgaatat
     3841 gtactcagga cttggtacaa aataatattc aaacatgatt ttagattgaa ctggtggatt
     3901 tgcatatcaa gagcaggatg atgcggtaaa atgctttccg tttatatagg ctacaggata
     3961 gacagaccgg tgggaaggcg ggtaaagaac ccccgcatcg tttaaatcca tatgttttaa
     4021 tcgttgtttc aggtgtcata aatttgcttg caaaggagaa cttttatgta taaacaagag
     4081 actatggacg gcaaccaggc cgccgcctac gcatcctatg cctttacaga agtggcggcc
     4141 atatttccta taaccccgtc tacccccatg gcagaaggcg tagacgaatg gtcagcccat
     4201 ggaaagaaaa atatttttgg acgggaggta aaggtagtcg aaatgcagtc ggaggcagga
     4261 gccgccagcg ccatgagggg tgccctccag gccggcaccc taggctcaac gtatacggca
     4321 tctcaggggc tgttgctgat gattccgagc ctttataaga tggccgggga attactgccc
     4381 ggggtgctcc atgtaagtgc cagatccata gccacccatg ccttatccat ctatggggat
     4441 catcaggatg tcatggcatg caggcagacc ggtgtagcca tgttagcttc cggcgatgcg
     4501 caggaggcca tggattttgg gtttatatca catctggcgg ctataaagtc caggattccc
     4561 tttattcatt tttttgacgg attccggacc tcccacgagt atcaaaaggt taggctggtg
     4621 gactatgagg atatcaaaaa gatagttgat tacaaggcca tcgaggactt ccgaaacagg
     4681 gctctaaacc cggaacggcc ggtcgtcagg ggtactaccc aaaatccgga tatctacttt
     4741 cagcagaggg agggggccaa ccccttttat gatgctgtac caaatatcgt gaaagggtat
     4801 atggacggta tggaagccat caccggccga agctacagac tctacgaata ttatggggcc
     4861 caggatgcgg aaaatgttat agtggccatg gcatctgtca cgggtacaat ctctgaggtg
     4921 gtggatcatt taaatgccgg gggagagaag gtaggcatgg ttaaggtgaa cctttacagg
     4981 ccgttctcgg aagaacattt ccataagatt ctcccaaaga ccatcaaaag gatagctgtt
     5041 ctagacagga caaaggagcc cggatccata ggggagccgt tgtaccttga tatagtcaag
     5101 gccttcaagg atgtagcaaa tgcccctacc atagtgggcg ggaggtatgg gctctcgtcc
     5161 aaggacacca ggccgtctca gatatttgcg gtatttgaaa accttaaaca ggataggcca
     5221 aaggacgggt ttacaatcgg gatagtggac gacgttacct ttaaatcctt gccggagacg
     5281 gagataataa acacggagcc tgcgggaact atcagctgca ggatatgggg tctcggctct
     5341 gacggcacag taggcgctaa taaaacagcc attaaaataa taggggacaa cacggaccta
     5401 tatgcccagg ggtatttttc ctacgatagc aagaaatcgg gggggactac cgtatcccac
     5461 ctgaggtttg ggccaaagcc tatcaaatcc acctatctgg tctacaatgc ggattatgtg
     5521 gcgtgccaca atcaggccta tgtgcatcac tatgacttac tcaagggatt aaagagggag
     5581 ggcaccttcg tactcaactg ctcatggaat atggatgagc tggatgagca tttgccggca
     5641 gatatgaaga ggtatattgc ccaaaacgca ataaaattct acattataga tgcgatgaaa
     5701 attgcatcgg aggtcggcct tggcaacaga ataaacatgg ttatgcaggc ggtattcttt
     5761 aagctggcaa atgttttgcc tgtagatgag gcgatcggat atctgaagga ttccatagag
     5821 gatatgtacg gaagaaaggg acaaaacata gtcgagatga ataatagggc cgtggacagc
     5881 tctttggagg ctctggtcca gatagaggtc cctgctgtct ggggcacagc cgaggacgat
     5941 gtgacggacg attcccatgt gcccgatttt gtagaaaaca tacagacccc catggccagg
     6001 caccagggtg acgagctgcc tgtgagcgca tttgtgggca tggaggacgg aacctttcca
     6061 atgggcacta ctgcctacga aaagcgaggc attgcgccta tgcttcccca atggcaagtg
     6121 gaaagatgca tacaatgcgg cagatgctcc tacgtctgtc cacatgccac tatcaggctc
     6181 tttctgttgg atgatgagga aaagaaaagg gccccggaga cattcgaaac caaaaaagcc
     6241 acgggccgtg gcatagacca ccttcaattt cgagttcagg tggatccctt agactgtaca
     6301 gggtgcgcca attgtgccga cgtttgtccc gccaagggta aagcactggt tatgcagcct
     6361 gctgagcagg agattaggat gcaagagggc aattgggaat ttgccatgac agtttctatg
     6421 aaggatgacc tcacaaatac aaataccctc aagggaagcc agtttagaag gccgcttctt
     6481 gaatttaatg gtgcatgtcc cggatgcggg gagacgccct acatcaagct cttgacccag
     6541 ctgtttggcg aaagaatggt gatttccaat gcgacaggtt gttcgtccat atggggggct
     6601 tctgcgcctt ctattgccta tactaccaat tggaacggca agggtccggc ctggataaac
     6661 ccactgtttg aggatgctgc agaatttgga tacggtatgg ccctaggcgc aggacagata
     6721 agggaaaagc tggccgatct tatgagaggg gccattgatt cgtcgttacc ttcggatata
     6781 aaggaagcct ttgaaaactg gctggattcg atgtactacg gtgaagaatc caaaaaggca
     6841 tcggaggcgg tccttagggt ccttggggac catgatccta tggataatgg ggtcgtgcag
     6901 gagatcctaa agagaaagga ttatctcgtt aaaaggtccg tatgggccat aggcggtgac
     6961 ggttgggcct acgatattga ctatggtggg cttgatcatg ttctggcctc aggagaggat
     7021 ataaacatct ttgtcatgga cacagagctg tattccaaca ccggcggaca gagttccaag
     7081 tcgacccccg cggcctgtgt tgccaaattg gcggcatcag ggaagaagac cgctaaaaag
     7141 gatttgggcc ttatggccat gacctatggc tatgtttatg tcgcccagat agccatgggc
     7201 gctgatatga accaaaccat aagggccata aatgaggcag aaaggtatag gggaccgtcc
     7261 ctgatcattg catattcgcc ctgcatcagc catggcataa aaacagggat gggaaccagc
     7321 atattacagg aaaagagggc tgtagatgcg gggtactggc atctatatag gtttaacccg
     7381 gatcttagag aaacgggaga gaacccattt gttttggatt ctaaaaaacc tacaaaagat
     7441 tacagagagc atatccacgg ggaggtacgt tattcacagc tcatgaacgt tttccctgaa
     7501 attgctgacg atatgtttga tctctcagcc cagcatgctg cggagaggta caaaagatat
     7561 ttaggattag cagagcatga aatcttttaa ccgccggagc aatcagtaaa aaatgggggc
     7621 cgcgtatcaa tggcccccat tctacttttg gttagtgtct acatactcct tagcgtttct
     7681 tatctgtatc tcgttgggca ggtttacttt cgaatcgggc ttgatgccct ctgcgtttgc
     7741 ccaagcggcg gtataatgat tttctatcgg taccaatgcg tttttgatct tgtatctacc
     7801 cttcgacata tcttccttca tttgcatacc tcctgctgta aagctggttc aaaatatcat
     7861 atccataggt gagtggctct agaccctctt atttttacca ctaagtaagg tttttatgtc
     7921 acaccaacgg taatgggatt tctcttccgg aatatacagg caaggaatac gtagactaaa
     7981 tttatcggca tgataatcgc aatggtcctc tgcagtttcg gtgtagaatt ttatagatgg
     8041 aattgtttat ctatgataga cctttttgta aaatccactt tattattaca tccagatttg
     8101 atagaataaa cactataaaa cacatctgaa tatttaacaa catccaaagg gggtatttaa
     8161 atgaaaaacc aaaaaccgga tgtagccgca tatctcaacc ggattggata tgacgggccg
     8221 ctggatggga gtgctaaagc tctagcaaag atacaagaag gtcatcttca ctctatacct
     8281 tacgagaact tcgatataat ggcggggata ccgttgtccc tggaggtaac ggatttattg
     8341 gataaaattg tgtacagagg taggggtggt tattgctttg aattaaatgc cctatacaaa
     8401 tggctcctag aggagttggg ttaccctacc atcagcttct ttgcgagatt ttggcgggac
     8461 gaacccaatg ctccgccaaa gaggaggcac catgtattaa aagtcgaggc ccaaggggaa
     8521 tactatcttt gtgatgtagg tgtgggcgga atcgttccca ggtggcctat aaagatggtg
     8581 gaagggctta tccaccgtca aggtgacgaa agctatcgct tagaacttga tgactattat
     8641 ggttgggtgc tatgcgagct taaagatgga aaatgggaat ggctttattc cttcaccgaa
     8701 gagccacagc ttccaaaaga ctatataatg gcaagctttt ggtgtgagaa gtcacctgat
     8761 tctattttta tagaagatgc catggtcgca gtgcgtacaa aggagggacg caacaccata
     8821 gccggcaagg agtttcgaat ttttatcggt gatgaggtcc atacctttgt ccccaagaca
     8881 gttgaagaat ttaccgatgc gttaattgag tattttggtt tggatatatc ccacatttcc
     8941 cataggatct gggatgtaaa ctttgaaaga tgacgattaa gggtttatgg ctatgttgaa
     9001 tggtttaata gtaattgata acatcttatc gtcccgacat aaaagatgga aaagatttta
     9061 aaattttgat ctgggtcaat gatttagtcc agatacgggt ttataattgg gtcatagacc
     9121 aaaaataagg gaggaacaga caatgaccac aaagacttac caattggaaa cactgacatg
     9181 ccctagctgc atggtaaaaa tcgaaggtgc tttaaagagg accaagggag tagaaaaatc
     9241 tagggtatta ttcaattcta gcaaagccaa agtgaccttt gacgaaaatg tagtcgattc
     9301 ggcaacaatt aaagaagcta tagagacatt gggctacgag gtgctgggcg agaaataaac
     9361 cggcttttag ttggaggcaa gattaggatc gatatggggg ttattatatg aagtgcaact
     9421 gcatgaaaag ggacgggaaa aactgcatcg aacttgtacc gatatttaac aatctgagtc
     9481 acgatgagat gatggagatc gccggaataa caggggacag gatctatgag aaagggaaca
     9541 tgatctatat ggccggggat gtcggcgaaa gcctatatgt catccatcga gggaaggtca
     9601 agatctacag gctgtccgat tccggaaagg aacaggtttt aagggtgctg ggtcccggtg
     9661 aatttatggg tgaacttacc ctgttcagcc acaaccccca tacggacaat gctgaggctc
     9721 tggaaaaaac aacagtatgt gtaataaacg gcgtagaaat aaggaatctt atgttgaagt
     9781 atcccgacat cgcctttaaa gtgctggagg aattgagcca gaggctggaa agggccgaaa
     9841 atctaataga gaacataagc cttcatagcg tagagcgcag gctggcagat accctattgg
     9901 atatggctgg tgacaagggc gaaattgacc taaagatgag tagaaaggat cttgcgtctc
     9961 atctgggtat gagcagggaa accttaagca gaaagctgtc gtccttccag gacgtgggat
    10021 tcatccagct cataggccat agaaaagtaa ttattctgga tgcggatgcc cttatggaga
    10081 tcgaatgata tctccggatt gtattaaatc atattttggg caaacaatag agtatagata
    10141 aacaactcta actataaaat ttatcataat gaaattgcac agaaacacag caattttcat
    10201 gccgataaat ttagtttcca tattgtttat ctataactac atcttttgtt ggaaggtggt
    10261 agaactctat gaacatgata gacggcaggg gtcctttttt tccctacccc tttaacctgt
    10321 atcccgatga gggtgaccag gacggcggta aggtagccga tgaaagatac catgtgtatg
    10381 ttaacgggga ttatgtaggg gataggttat ccattgccca gggggacgtc ggatggcaag
    10441 cggtagaagg ctacctaaag ggcagagatt ttagcggata tcgggtaacc aaggacgggg
    10501 atcaagtata tgtagacgtt cagaacgagg aagaggcaga ggacatacgg agccatttaa
    10561 gtatttacac ccgaatagag tagagaaggt gggaaccttt caataaatat agccttgata
    10621 taggattaag accccatatc aaggctaatc ttacgctatc catctttatt ctcttaccat
    10681 taagatgata cggcgacggc atccctcgtg aggttctgaa tccatttaaa cctgttaacc
    10741 ttaacgacgg ccaccccgca ttttaggatc tggtcacact tcagacatgc gaacaccact
    10801 gcgggagggg cattaaagac gtaatgcgct ataagcgccg atggtataac aacgagccat
    10861 acaaagatca aatcgttgat aagcacgaaa tatattgcgc cccctgctct tactataccg
    10921 gttagggatg acatctggta ggaggtgcct attatggtaa cggataggac gacaattagc
    10981 tgcctcgaga gggcgatagt atcttcggat atattatata gcagcaggat ataatccttt
    11041 atgagatata aaattgcacc tgagataaaa cctataatca gaaacagtag ctgcagtttt
    11101 tttgcgtact cctttacctt ctcatattcg ccggcgccca cggtctgtcc gataatcacc
    11161 gaggtggcac ccgatgttcc gtaacaaccc acggatatca atgagaagac tatattggcg
    11221 atactgacag aggcttgtgc cgtagatccg agccttccta tgatgatgcc ttggactgtg
    11281 aggttgagac cccaaaatat atcccccagt atgacgggga acccgtattt gaaaaaatcc
    11341 ttcaataata tatagttagt tttaatt
//
LOCUS       DASRNX010000026        11838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_587906,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000026 DASRNX010000000
VERSION     DASRNX010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11838
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587906"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFD89_00980"
     CDS             complement(<1..213)
                     /locus_tag="VFD89_00980"
                     /inference="COORDINATES: protein motif:HMM:NF016215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HZJ56799.1"
                     /translation="MVKIHRALFRNSVHQGLTLDQVYELLLGFLRRAPHNEYRLSIGT
                     DSQVGADKTVFVTAIHLHKVGRGATGF"
     gene            complement(448..1965)
                     /locus_tag="VFD89_00985"
     CDS             complement(448..1965)
                     /locus_tag="VFD89_00985"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZJ56800.1"
                     /translation="MGVISVKRRYLIGAVILVILCIIVPGFYHFLWGSGRALNRLNPT
                     RYEMDVTFNPNSKKIECGQRTTYINNTKTDLWTLYFHVYPNAFKSEDKPVFPAEEMDR
                     AYPNGFSPGFMEFGEVLIDGRSTAYALEGYSDDKLMIPLDEALKPGDRLDIYLEYSVA
                     IPNSPGRFGYSDNTYNLGNWYPILCVYDERGWNLEPYHTLGDPFYSDVSSYDVKIIAP
                     LEFVIASTGEVVKVVRESEQNIWELKGQNVRDFAWVASQRFQASSVTVGETTVYSYYH
                     TEEGGEKALEFGASALEIFNGLFGVYPYRNLSIVQTDFFIGGMEYPRLVMIDESLYDG
                     NQDDWLELVTVHEVAHQWWYGLVGNDEVNDSWLDEGLTEYSTILYYGQRYGRDEEEQK
                     YQTLINKGKLQLFRIYRDSGETDETIHRPLYQFKDWVEYDSLVYGKGAIMFHELRQEM
                     GDTAFFGVLKEYFKENMLQNAKPEDLYRACEKITGKAWDDFFRQWLYDDYGQKAS"
     gene            complement(2146..3444)
                     /locus_tag="VFD89_00990"
     CDS             complement(2146..3444)
                     /locus_tag="VFD89_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ56801.1"
                     /translation="MEEKIRIDGEKNVSPGDSIPRPSIDESWERCSQYGLDGINGPKV
                     FKVSNQRIEQLLDRYKDVISAADPYIKGLTLALPKNESIIGLASDEGVLLKVRDNVNG
                     IMNLGFQRGFVHQEMYMGTNAIGMCIETKSRAMVWGEEHFFKALGDWVGFAAPINMDD
                     NPLGAIIFAMVPVGLAYRGFLEVISMAAHGIDMQLRLAHDNKRLVNLQLTTQNGRDDI
                     LEASSIVSHEVKNSLANISAYIQLLQLDRAITNISGRKILNEISRVTRMLDDFKLLSG
                     HKYDTTQVHPLSGILQLVIDVMASKAQLNDVDIIFDSPDESIHIKADKNSLQQVFINL
                     IDNAIDSMKDGGTLTIGLQTESSPNMAIISFKDTGSGIPPDEIPNIFKIYQTTKVSGS
                     GLGLHICQTIVRFYGGSIGVESTLGEGTTFVVKLPEVFDK"
     gene            complement(3494..3718)
                     /locus_tag="VFD89_00995"
     CDS             complement(3494..3718)
                     /locus_tag="VFD89_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56802.1"
                     /translation="MGKKRRLIRLFGNILFILAIGLSLYLLIGLFIVRRSLPPGVCPI
                     DNKRPLMYIAIALALSSFVLTFIENRIKDR"
     gene            complement(4004..4129)
                     /locus_tag="VFD89_01000"
     CDS             complement(4004..4129)
                     /locus_tag="VFD89_01000"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HZJ56803.1"
                     /translation="MNEVMVETVKIMGIGMGTVFFVLMLFYVMVRGLQIVFPPEE"
     gene            complement(4141..5247)
                     /locus_tag="VFD89_01005"
     CDS             complement(4141..5247)
                     /locus_tag="VFD89_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HZJ56804.1"
                     /translation="MEKLLEGVQGMTWQQGVMILVGLILIYLAIKKEYEPMLLLPIGF
                     GAILANIPGSSAIGEGGFLTILKEAGITTELFPILIFIAVGAMIDFSPLLKQPVMLFF
                     GAAAQFGIFVAMFLAASTGVFSIREAASIGIIGAADGPTSIYAASRFAPNLLGPISVA
                     AYSYMALVPMIQPPIIRALTTKRERLIRMDANYDAKVSKRVLILFPIVVTLVAGIIAP
                     EGVGLVGALMFGNLIRESGVLERLSKSAQNELANLVTILLGITIGSTMVASEFLKPTT
                     LLILGIGLLAFVFDTAGGVLFAKLINLFLRTKVNPMIGAAGISAFPMSARVIQKMALK
                     EDPSNFILMQAIGANVAGQIGSIIAGGVLLSLLR"
     gene            complement(5458..6897)
                     /locus_tag="VFD89_01010"
     CDS             complement(5458..6897)
                     /locus_tag="VFD89_01010"
                     /inference="COORDINATES: protein motif:HMM:NF023435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HZJ56805.1"
                     /translation="MRSSWIKGLGVSISLMVLMGTLLGCSIFSSNSTPLVDGVIDTSK
                     HIKLSMYLMGPPSAEPAWDFQGVVDELNDNIGNKMNAYVDITLIPADEADVEYPLIFN
                     SGTGFDISYVSTAGEPGYFSLTSDEVLLDIDELLPTYGKKLWEGIGPDRWEDTRYEGK
                     IYAVPMDGYEYRINGFILGGDIHNEYEPGPILSIGDIKDYLDALTEENGDKSVIDISE
                     GSAMGLYNMLVDLHKTWIPVPGIPESSLYLVSSSGSREGNILHPAFSWDFVEFADMMK
                     EWADNGFWQGDVLAADNDIAGRPNAGRDALMFGDFNRFLTIDPRLGDPSVQKEERLKF
                     IPFWGEGKGIIKAGSAKNALGIGAGSRNPERALMLIDYIIGSEVADQLLGYGYKGKIV
                     DYSSFWVDPDNREVISVKDPYDKFVFDDSLVKEEIESILMINSQYGIPILLGKAGDPK
                     AAVQRYRQELEVAGIQKVIDTLKEQLKNF"
     gene            complement(6917..8671)
                     /locus_tag="VFD89_01015"
     CDS             complement(6917..8671)
                     /locus_tag="VFD89_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010295409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ56806.1"
                     /translation="MLMHFISYYKPHRKLFIIDLICAFVISVIDLFFPILTRRIVQTI
                     IPGGHINTFWLFIGIMVIMYIARTALRFVVEYWGHILGIRIEYDMRKDLFSHLQTLSF
                     RFFDRNRTGHLMSRMVNDLAEIAEFAHHGPEDLFLSLVMLVGSFFVLIRIEWRLALMV
                     YAFIPVMVWYGIDKRKDMNKAFRDMRVKLADINAQLESSISGVRVSKSFTNEDHERDK
                     FEKGNVKFRGSKYWAYRFMALFHTGIELMSALLNTIVIAAGGYMIFKGSIDLADLLAF
                     IMYIGLFMQPVRQLSNFVQQFESGMTGIERFVEIMEEHPDITDAPNAKELSNVKGDIG
                     FHNVNFSYDNNERVLDNINIGIDSGKTVALVGPSGGGKTTMCHLIPRFYEVNGGYISI
                     DGHDIKDYTLKSLRRNIGIVQQDVFLFAGTIGENILYGNVEATYEEMIQAAKNANIHD
                     FIMSLPDGYDTYAGERGVRLSGGQKQRISIARVFLKNPPILILDEATSALDNETEIKI
                     QQALENLSKGRTTLVIAHRLSTIKNADEIFVLTESGVEEKGDHETLLEMGGIYSNLYR
                     SQFKDLDPEEMIKPDMAG"
     gene            complement(9049..11172)
                     /locus_tag="VFD89_01020"
                     /pseudo
     CDS             complement(9049..11172)
                     /locus_tag="VFD89_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434056.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            complement(11230..>11838)
                     /locus_tag="VFD89_01025"
     CDS             complement(11230..>11838)
                     /locus_tag="VFD89_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
                     /protein_id="HZJ56807.1"
                     /translation="AFVLSGLVYCRSCGYSMGNTRNGISKKEFLKKCQTRDSLGNRTC
                     NNPGVNSYAIVKGIFQQLDEYEREIKKRGEDMDTNETELLKVALTKKHKEIEKQRGAL
                     DTLIEMREEQEITREKFLERKEIREFNIAQLRNEIGELQDRINQREQTTNERKLKNIR
                     EFRDKWGKSKSSEQRNQFLQTIIDRIDYQREGDNIDIHINFR"
BASE COUNT         3216 a         2985 c         2216 g         3421 t
ORIGIN      
        1 aaaacctgta gctccgcggc caaccttgtg aaggtgtata gctgtaacaa agaccgtttt
       61 atctgcgccg acttgggaat cggttccgat ggataagcgg tactcattat gaggcgcccg
      121 tcttaaaaaa ccaaggagca actcatatac ctggtctaag gtcaagcctt gatgtactga
      181 gtttctaaac aatgctctat ggatcttaac cacttcccaa aaagaatttg aaatattacc
      241 ttggtagcat ttattatatc acacgtgcaa gccttaattg gcttgcttca caccgattca
      301 ataaatgcat cggataaaac cccgagggtt ttattacctt aattgcattt attatatcat
      361 agaatcccca aaattgttac acaagtttta cgaatgtgtt aagcaaagat ccgattccat
      421 ggatttgcac ctatgggccg gggtaggcta actggctttt tgtccataat catcgtatag
      481 ccattgtctg aagaaatcgt cccaggcctt tccggtaatc ttttcacagg ctctgtatag
      541 atcttcaggt ttggcgtttt gaagcatatt ctctttaaaa tattccttca gaaccccaaa
      601 gaatgccgta tcccccatct cctgtcttaa ttcatgaaac atgattgcgc cctttccgta
      661 gaccagggaa tcatattcca cccaatcctt aaattgatac aggggtctat gtatggtctc
      721 gtctgtttcc ccgctatctc tgtaaatcct gaataattga agcttgcctt tgtttatcaa
      781 ggtttgatac ttttgctctt cctcatcccg gccataacgc tgaccgtagt acaagatagt
      841 ggaatattcg gttaaaccct catccaacca tgaatcatta acttcatcgt tacccacaag
      901 tccgtaccac cattgatggg caacctcatg gacggtaacc aattccaacc aatcgtcctg
      961 gttcccatca taaaggcttt catctatcat cactagcctt ggatactcca tcccgccaat
     1021 aaaaaagtcc gtctggacaa tggataggtt cctatacggg tacacgccaa agagcccgtt
     1081 gaagatctct agggcagatg ccccaaattc caaagccttt tcgcccccct cttcagtgtg
     1141 atagtatgaa tacactgtgg tctcgcctac ggttacagaa gatgcttgaa acctttgact
     1201 ggccacccaa gcaaaatccc tgacattttg gccttttaat tcccagatat tttgctcact
     1261 ctcccggaca accttaacca cttccccggt agaagctatc acaaattcca aaggtgcgat
     1321 aattttcaca tcatagctgg atacatcgct atagaacgga tctcccaagg tgtgataggg
     1381 ttccagattc caccctctct catcatatac gcataggatg gggtaccaat tccctaagtt
     1441 ataggtattg tcagagtaac cgaacctacc cggagaattg ggaatagcta cggaatattc
     1501 aaggtaaata tccagcctat cccccggttt cagggcctca tcgaggggta tcatcagctt
     1561 gtcgtcgcta tagccctcga gggcgtaggc tgttgaacgg ccgtctatca atacctctcc
     1621 gaattccata aaacccgggg aaaatccgtt gggataagcc ctatccatct cctctgcggg
     1681 gaaaactggc ttatcctcgc ttttgaaggc attaggatag acatgaaaat agagggtcca
     1741 cagatccgtt ttggtattgt ttatgtatgt agtccgctgc ccacattcaa tctttttaga
     1801 atttggatta aatgtcacat ccatctcgta tcgggtagga ttcaatcgat ttaaagccct
     1861 tccgctgccc cataaaaaat gataaaaccc cgggactata atgcatagga tgaccaaaat
     1921 gaccgcaccg ataagatatc tcctcttaac ggaaataacc cccataccat cgcctctttc
     1981 aaaattttat acctattatt tctcctcttt ggtatgggat tcttgctaag cgctaaaaga
     2041 gcctatagat gggcaatatc ccatatattt tgatttgtcg tatgcccgtg ggctgcaaag
     2101 aatcatttat atgtatatta aataggtttg atatgtagaa tagatttatt tgtcaaagac
     2161 ttcgggtagc ttgaccacaa aggtagtgcc ctcacccaat gtgctctcaa cccctatgct
     2221 gccgccataa aatctgacga ttgtttggca gatatgcagc cctaggccac tgccgctcac
     2281 tttggtggtt tgatagattt taaatatgtt cggaatctca tcggggggta tacccgatcc
     2341 ggtatcctta aagcttataa tagccatatt agggctggat tctgtttgca atcctatggt
     2401 tagggttccg ccatctttca tggaatcgat ggcattatct atcagattga taaatacttg
     2461 ctgaagtgaa ttcttatcgg cttttatatg tatactctca tcaggtgaat cgaatatgat
     2521 atcaacatcg tttaattgcg ctttagatgc cattacatct atcaccaact ggaggattcc
     2581 gcttaacgga tggacctggg tggtgtcata cttatgtccc gatagcaatt taaaatcatc
     2641 caacattctt gttacacggg agatctcgtt taaaatttta cgtccgctga tattggtgat
     2701 ggccctgtcc agttgaagca gctgtatata tgcactgata ttggcaaggg aattcttgac
     2761 ctcatgggat actatagacg atgcctctaa gatatcgtcc cgcccgttct gtgttgtcag
     2821 ctgtaggttc accagtcttt tgttgtcatg ggccagccgc agctgcatat ctatgccgtg
     2881 tgccgccatg gatatgacct ctaaaaatcc cctataggct aatccgacgg gcaccatggc
     2941 gaatattatc gccccaaggg ggttatcatc catatttatc ggggctgcaa aaccaaccca
     3001 atcccctagg gctttaaaaa aatgttcttc accccaaacc atagcacggc ttttcgtttc
     3061 aatacacatt cctatagcat ttgtccccat atacatctct tggtggacaa aacccctttg
     3121 aaaacccagg ttcataattc cgtttacatt gtccctgact ttaagcaata caccctcatc
     3181 gcttgcaaga ccgataatgg actcgttttt gggcagcgct agggtcaaac cctttatgta
     3241 tggatcagcg gcacttataa cgtccttata tctatcaagg agttgttcta ttctttggtt
     3301 gcttacctta aagactttgg gcccattaat tccatcaagc ccatactggc tgcatctctc
     3361 ccaggattca tcaatagacg gtcttggaat gctatccccg ggtgatacat ttttctcgcc
     3421 atctattctt attttctctt ccacaggacc aagcccccta aataaaaaca atgcgttctt
     3481 taggccccta ctatcacctg tctttaattc gattctctat aaacgtgaga acaaaggatg
     3541 acagggccaa tgctatggca atatacatta aaggtctctt attgtcgatg ggacaaaccc
     3601 ccggaggcag actccttcgc actataaaga gacctatcag aaggtatagc gaaaggccta
     3661 tcgccaggat aaagagtata tttccaaata atctaatcaa tcttcttttc tttcccatga
     3721 tgatcccctt ttacgagccc tttaccccta gtctagcata taattgtatg ggaatgcaaa
     3781 tagtgctttc cccgtaccca taccacggtt aacatccata aatcaagccg gatcggcccc
     3841 atataggata tccagaattg ggcagaccca ccccattagt gcctgctgtg tatgcagctg
     3901 ggctatagtc cttatcggtg cgaagccatt ttataaattg agcctacatc gtataataaa
     3961 aataaaaccc acgcatgcag cgtgggaatc tgtagcaaat cccttattct tcggggggaa
     4021 agacgatttg aagcccccta accataacat aaaacaacat gagcacaaaa aaaaccgtac
     4081 ccatacctat ccccataatc ttaacggttt ccaccatgac ctcattcatt atactacacc
     4141 tcaccttaac aatgataata aaactccgcc cgcgatgatg gatcctatct gccctgctac
     4201 gttagcacca atagcctgca tcaaaataaa atttgacgga tcctccttta aggccatctt
     4261 ttgaataacc cttgccgaca taggaaacgc ggatatgccc gccgccccga tcatagggtt
     4321 gacctttgtt cttagaaata ggtttataag cttagcgaac agaacccccc cggccgtatc
     4381 aaagacaaaa gccaaaagac cgatacccag aatcaacagg gtagtgggct ttaaaaattc
     4441 ggaagctacc atagtagagc ctatggttat cccgaggagt atggttacca ggttcgccag
     4501 ctcgttttgg gctgatttag aaagcctttc taaaaccccc gattccctta tcagattacc
     4561 aaacatcaag gctcctacca gaccgacccc ttcaggtgct ataattccgg ctaccaaggt
     4621 tacgactatg ggaaacagaa tcaataccct tttggacacc ttggcatcat agttggcatc
     4681 catacgtata agccgttccc ttttagttgt aagagcacgt ataatcggtg gctgaatcat
     4741 aggtaccagg gccatatatg aataggctgc cacggatata ggacccaaca ggtttggggc
     4801 aaatcgggat gctgcgtaga tggaagtggg tccgtctgcc gctccgatta taccgatgga
     4861 ggcggcctcc cttatgctga atacccctgt agaggcggct aggaacatag ctacaaaaat
     4921 cccgaactgg gcggcggctc caaaaaacaa cattacaggc tgctttaata agggactaaa
     4981 gtctatcata gcccctaccg caatgaatat aagtatggga aatagttctg tcgtaatccc
     5041 ggcttccttg agaatggtta agaatcctcc ctcccctatg gccgatgagc ccgggatatt
     5101 agccagtatt gcaccaaacc ctataggcag caataacatt ggctcgtact ccttttttat
     5161 agccaaatag atgagaatca gaccaaccag tatcatgacc ccctgctgcc aggtcatgcc
     5221 ctgtactccc tcaagcaact tttccatgct tttccctcca tctacatgat aaacaaaaaa
     5281 gactttcgcc cggctataat aagccagaca aaagtcttat cctcgaattg gtatccgaat
     5341 cccgaccggg cgattgttcg ccgtactgac gaccgggata atcgcaaagg cgatggatta
     5401 ctccccttta tcctctctaa tcatatgccc ccttaaaggc attgtcaata gacaaactca
     5461 aaaattcttg agttgttcct ttaaggtgtc aatgaccttt tggataccgg caacttccaa
     5521 ttcctgtcta tacctttgga ccgccgcctt tggatctcca gccttcccca acaggatggg
     5581 gatcccatat tgagaattta tcataaggat actctcaatc tcctctttta caaggctgtc
     5641 gtcaaagaca aacttgtcat agggatcctt tacggaaatg acttcacgat tatcgggatc
     5701 aacccaaaat gaggaatagt ccacaatctt ccccttgtat ccataaccta aaagctgatc
     5761 ggcaacctcg ctcccaataa tataatctat cagcataagg gccctttccg gattccttga
     5821 ccctgcgcct atcccaaggg catttttagc ggatccggcc tttataattc ctttaccttc
     5881 cccccagaag ggaataaatt taagcctttc ttccttttgg acagagggat ctcccaacct
     5941 tggatctata gtcaagaacc tattaaaatc cccaaacatc agggcatccc tacccgcgtt
     6001 aggcctgcct gcaatatcat tatccgctgc gagtacatcc ccctgccaaa aaccattatc
     6061 cgcccattcc ttcatcatgt ccgcgaactc cacaaaatcc cagctgaagg caggatgtaa
     6121 gatattacct tccctgcttc cggagctgga gaccagataa aggctggact ccgggatgcc
     6181 gggaacggga atccaggttt tatggaggtc gaccagcata ttataaagcc ccattgcgct
     6241 cccttcgctt atatctatga ctgatttatc tccattctct tcagtcagtg catccagata
     6301 gtcttttata tccccgattg ataatatagg tccgggttca tattcattat gaatatctcc
     6361 accaaggata aatccgttga tcctatactc atacccgtcc atgggcacag catatatctt
     6421 gccttcatac cttgtatctt cccatctgtc aggccctata ccctcccata gtttctttcc
     6481 ataggtgggc aaaagctcat ctatatccaa taagacctca tcagaggtaa ggctaaaata
     6541 accgggttct ccggccgtac ttacatagct tatatcaaag cccgttcccg aattaaatat
     6601 tagcggatat tcaacatccg cctcgtccgc gggaataagt gttatatcca catatgcgtt
     6661 cattttattc ccgatgttgt catttagctc atcaacaacg ccctgaaaat cccatgcagg
     6721 ctcggcagac ggcggaccca tgagatacat ggatagcttt atatgcttgg atgtatcgat
     6781 aaccccgtcc accaagggag tggaatttga agaaaatata ctgcacccca acaacgtgcc
     6841 catcagcacc ataaggctta tagaaacacc caagccttta atccatgaag acctcatgcc
     6901 ctttacctca ctaaccttaa ccggccatat ccggcttaat catttcctcg ggatccagat
     6961 ccttaaactg agacctgtat aggttggaat agataccgcc catctccaac aatgtctcgt
     7021 gatcgccctt ctcctctaca ccgctttcgg ttaaaacaaa gatttcatca gcattcttta
     7081 tagtggataa cctatgagct ataaccaatg tggtcctccc ctttgaaagg ttttccagtg
     7141 cctgttggat cttgatctcc gtctcgttat ccagggcaga agtcgcctcg tccaatatga
     7201 gaatgggagg attctttaga aataccctgg ctatggatat tctttgcttt tgcccgccgg
     7261 ataaccttac gcccctctca ccggcatagg tgtcatatcc gtcgggaagg ctcatgatga
     7321 aatcgtggat attggcgttc tttgccgcct gtatcatctc ttcataggtg gcctcaacat
     7381 tcccatacag gatgttctcc ccgatagtcc cggcaaagag aaatacatcc tgctgcacaa
     7441 taccgatatt ccttctcaag gatttaaggg tgtaatcctt tatgtcgtgc ccgtcaatgg
     7501 atatatagcc cccgttgacc tcataaaacc gggggatcag atgacacata gtggttttcc
     7561 ctccgccgga agggcctacc agtgcaacgg ttttccccga atctatgcct atattgatat
     7621 tatccaaaac cctttcgtta ttatcatatg agaaatttac attatgaaat ccgatatcgc
     7681 cctttacatt acttagctcc ttggcgttgg gtgcgtctgt tatatccgga tgctcttcca
     7741 taatttctac gaacctttca atgccggtca tccctgattc aaattgttga acaaagttgg
     7801 acagctgacg aacgggctgc atgaagagtc ctatatacat gataaatgcc agcagatccg
     7861 ccaaatctat gctgccttta aagatcatat atcctccggc tgctataact atcgtattga
     7921 gcagtgcaga catcagctct attcccgtat gaaagagtgc cataaaccta tatgcccaat
     7981 acttggaccc acgaaatttg acattgccct tttcaaactt gtccctttca tggtcttcat
     8041 ttgtaaagga tttggatacc cgtacaccgg aaatactgct ctctaattgg gcgttgatat
     8101 cggcaagctt taccctcata tccctaaagg ccttattcat atccttccgc ttgtcgatac
     8161 cgtaccatac cattacgggt ataaacgcgt ataccattaa ggccaagcgc cattcgatcc
     8221 ttatcagcac aaagaaggaa ccgacgagca ttactaagga taaaaacaaa tcctccggac
     8281 catggtgagc aaactctgca atctctgcca ggtcgtttac cattctggac atcaggtgtc
     8341 cggttctgtt cctgtcaaaa aaacggaagg acagggtctg cagatgggaa aataaatcct
     8401 tacgcatatc gtattctatg cgtatcccca agatatgacc ccaatattca accacaaacc
     8461 ggagcgctgt ccttgcgata tacataataa ccataatccc aataaacaac caaaatgtgt
     8521 tgatatgtcc gccgggtata atggtctgta ctattcttct agttagtatc ggaaagaata
     8581 gatcaataac tgagataaca aatgcacata tcaaatctat aatgaaaagc ttccgatggg
     8641 gtttatagta ggatatgaag tgcataagta tcttcttgtt cttactcaaa taacatttct
     8701 cctctcagta caacaaatag actatagata tacaatgtat attatatgga taattcgcaa
     8761 taaaatcaaa cactaattga tggctttggt cggtgaatta acccccggtt taataggtgt
     8821 caaagaataa tacctagaac gcgcaatgca aaaaatgtcc cctttgccct gggaatggct
     8881 aaataggcgg ggacataagc ttttgattcg tagaaattca tgcccataat taaaatacaa
     8941 ggattttagg cgatttggat tagttttgga ccatttggac ccattttcgt acaatatcgt
     9001 atggaacaaa aaataaaaga tactagttgc agcaattgaa cattggattt agttatttcc
     9061 gtaatcaggt agcagctgcc ttctaaaccc ttctacatcc acaataggtg ccttacaggc
     9121 atagttttca cagatgtaag cagtagcact ccctttcacc ggaatttgat ccgccgtgta
     9181 cggtgccaag ccttccaaag aaccgtcttt tatggccgta tccttaaaca ggactatcgt
     9241 ttggggtaag aaagcttttt ggataaggtc gatcatcctt ttcatatccg ggtccttatc
     9301 atctccggca aggacaacct ccctggttgg atataatcca aatatagcag ctgttagaaa
     9361 gtgggtatgg gcagagggat tactgttcac cgcccccgca aaagcgttta gctgggcata
     9421 ggctcttccc tctagatggg aatcccccgt taggcgggcc agacgcagca tatttaaggt
     9481 agcaacagaa tttcctgagg gcgttgcccc atcatatacc tctttcggac gggctatcaa
     9541 ttgttcaccg tcccttgcgt acagaaaaag accgctattt tcatcatcgt tgaacaaatt
     9601 cagcatttca ttattaaatt ttagagcctg ctgaagatat tctgcattaa aggtagtatg
     9661 gtacaactct ataaacgccc atacgagaaa agcataatca tctacatagg ccagatgggc
     9721 cgcctccccg tcccgatatc tggccagcaa tctaccgtcg ccattgaccg agttggccag
     9781 tataaaatct agagcccttt ctgccgtctc aacatagacg ggcttatcaa gaatcctacc
     9841 ccccatagcc aatgctgcta tcataagtcc attccacgaa gttagtatct tatcgtcctt
     9901 atgggggtgt actcgttttt tccgtgcctg aaaaacctta tcccgtaaaa gggcaatctc
     9961 ttcttgttcc tcaccgaggg agccccccga tgctattgtt tcgtcatacg aaccctcata
    10021 gaaatcgttg ttctcagcaa tcctattggg aatactcttt ccttcgaagt tccctttttc
    10081 gatgatatca taatacttgc aaaatctttt tccctgctct tcacctaaga tccccattat
    10141 ctccctaggc gtccacacat agaacttacc ctctattcct tcagaatccg catcttcggc
    10201 ggaatagaag cccccttggg gatgggtcat atcccttagt atatatgtaa agattttttc
    10261 ggctaccttc gcataccctt cattctgggt tacttgatag gcctctagat atgctatagc
    10321 caatagcgca ttatcataga gcattttttc aaaatgggga accagccact tctcatcaat
    10381 agaatatctg gaaaaaccgt atcctatatg gtcaaaaatc ccgcccctat acatcccgtc
    10441 caaggtcttc tccacaatgg acagggcccc gccctctcca gttagcttcc aatacctgag
    10501 caaaaatgac aggttgtgag gggttggaaa tttgggtgca tgcccaaacc cgccgtaaat
    10561 accatcaaag gcacttttaa gctccccgta ggcatgatgg atggattcct tcgccagctc
    10621 atcccgtccg gcacctgtat taaagtaggg ctctatatcc tctactatgg aattcgccga
    10681 ttctatcaaa tcctccctat gggatcgcca attcttgcct atcctatcca gcagttccat
    10741 caagcctatc ataccccgcc gccccttttt gggaaaataa gttcctgcaa agaatggttt
    10801 ctggtcgggg gtcataatga tggtaagggg ccagccgcca tggcctgtta aggcttggca
    10861 tatggtcata tatatgtgat ctatatcggg cctttcctcg cggtctacct taatggatat
    10921 gaaatgctta ttgagaatat ccgcaacctc ttcatcctca aaggactcgc attccataac
    10981 atggcaccaa tgacaagtcg gtaaaataaa ctacgaatac ccaatagata aaaatatagg
    11041 tttgtcctct ttcttggcct tggcaaaagc ctcatcgcac caaggaaacc attctacggg
    11101 attataagcg tgttgtaaaa ggtaaggact tttctcttca ataagtctat tgggtatatt
    11161 cgttgttttc atggcaccac ctcctttatc tctcttacat agttatttgg ttttttgcac
    11221 attatacgcc tagcgaaaat ttatgtgaat atctatatta tctccttccc tttgataatc
    11281 tatcctgtca attatagttt gcaggaattg attcctttgc tctgatgatt ttgatttgcc
    11341 ccatttatca cgaaattctc gtatgttttt tagctttctt tcatttgtag tctgttctct
    11401 ttgatttatg cgatcttgca attccccaat ttcgttcctc agctgggcta tattaaattc
    11461 ccgaatctct tttctttcta aaaatttttc ccttgttatc tcctgctcct ctcgcatttc
    11521 tataagtgta tctagggccc ctctctgttt ttcaatctcc ttatgcttct tcgttaatgc
    11581 tactttgaga agctcagtct catttgtgtc catatcctcg cctctctttt taatctccct
    11641 ttcatattca tctaactgct gaaaaatccc ttttacaata gcataggaat ttacccctgg
    11701 attgttacaa gttctattac caagtgaatc cctagtctga cactttttca aaaactcctt
    11761 cttacttata ccatttctgg tgtttcccat actatatcca cagcttctgc aataaaccaa
    11821 tccggataat acaaaagc
//
LOCUS       DASRNX010000027         7654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_57638,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000027 DASRNX010000000
VERSION     DASRNX010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7654
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57638"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFD89_01030"
     CDS             <1..550
                     /locus_tag="VFD89_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HZJ56808.1"
                     /translation="FIPCLDPLDEEPGGYPYSLTPERFTTFLKTLFDAWYDDIKKGNM
                     ISIRYFDNLVGMIMGYPPENCGMSGVCQVQFVVEADGGVYPCDFYVIDGWYMGNLKTQ
                     GFDELHKSQAAREFVEVSKYIDPKCRECRWVNLCRGGCRRNREPFIDEKPALNYFCSS
                     YMDFFEYATPRLYELARAFAGR"
     gene            561..2039
                     /locus_tag="VFD89_01035"
     CDS             561..2039
                     /locus_tag="VFD89_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02677 family protein"
                     /protein_id="HZJ56809.1"
                     /translation="MSKQLTLDKPLVEASYLTAPNFFRYRSILRFFYLQHQRMKYWMV
                     RDEVFSRLREMEGLRDYTLDQCQQDLDTLVDWKNLMAGQDAGKVQTIEEFKNRRFRYQ
                     LTPYTIEIERLMVRLESTSGIGGSLEANLFERISNRIREIPGIKEESPSRVYGWWQDL
                     ESDFQRLNQNATDYIARLEGGEAEELMNTLAFLTYKDGIIEYLRTFIRELQRYGPIIE
                     GDLRQVSEDTTGKLLDLVSSHHMTIPRMEQDFTEDEYREEIRSRWLNLALWFVGEGDE
                     ESESYRLLDITNGIIRKITRYAYRIAERQNRSISRQGEYMHLCNLFADCRSIDDANSL
                     SAAVFGAFYTRHVHGSFERETESISSGIWEEYPFEVQLKPRTRQYRERSHTGSIVSRE
                     TEKKRAMEEYLERMREEQEILAQYIEGNRLSFINLPEIEPFVRNTLLRWVGRAMANKE
                     RMGKTEDGRTYRVHIDKKSPRMTLRCQDGNLEMPPMIMEFLS"
     gene            2054..3217
                     /locus_tag="VFD89_01040"
     CDS             2054..3217
                     /locus_tag="VFD89_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02678 family protein"
                     /protein_id="HZJ56810.1"
                     /translation="MEESVLIEDIMTLLENFWILREKDDETYYRLKDNEARLKPFLEE
                     KLGYRMHINPYLIKLDKIPGEAETWMGIESFKTPMDYCFLLLLLSFLEDKGLEEQFVL
                     SDITEYIQSMYPEQDTIDWTIFQHRRSLVRTLGFARDMGIMKINDGDEGEFSRTQDAE
                     VLYESTGISRYFLRPFTRDITEMNGIDEILGSEWVDMDEERGIIRRHRVYRRLVLSPA
                     VYSAGNDDQDFYYIRNYRNTIQRDIEEYMGAWLHVHRNTALVMVNESYGRGDRFPAHN
                     NLSDIALQLSTVLRRQVDEGKRTPGADGSIVMTQVEFRGLLQDCHREFSHGWYKSYRE
                     MNMEKLMDEVKEYMMRWKMLGEDQRLKTIKILPLAVKFSGEFPKGYREGDKKR"
     gene            3214..7383
                     /locus_tag="VFD89_01045"
     CDS             3214..7383
                     /locus_tag="VFD89_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02680 family protein"
                     /protein_id="HZJ56811.1"
                     /translation="MSRWMLHRMGLVNFWFYDEEEFDFANGKLLLRGSNGSGKSVTMQ
                     SFIPLVLDGNKSPERLDPFGSRARKLEDYLLGEEGARSHEERTGYLYMEFRLGDLEQY
                     LTVGMGLRARRGRPVDFWGFCITDGRRIGKDITLYREGISPEDGQESRIPLSKSQMRE
                     LAEAGGVFVTGQKDYMEMVNRYIFGFDTVEEYDELIKLLIQLRSPKLSKDFKPTVIYE
                     IMNSSLPALSDEDLRPLTETIENMDQIKLHLDQLLKNQKAVSKLKEEYHRYNRLTLFE
                     RARAFNEGKLKQDEIRDKIKALEVNLEKDAQEIALLSESIASLEHEEEALRLKERQYL
                     EHDAYKLEQQWVEQGHSLRDMEANKTQKQGVLKEKQKREWRLKAELGELEAKMATLQR
                     QGQEILGEMDDLAEDIRFHDHEYDTIEFNRTGRKPFDFTAWNQDVLLYSQKLKEGRLA
                     LEAEIEANRIYDNLLKEQDTLVSGRDAASREEEKASEMVDTSRQGYMALIKSWAEENR
                     IFNIFGENILAFNQLVLRYGEGKDLGDLQELLNSLYYPQFQKLDREKISLEHTRDQLA
                     IEINDLMEELSKWKNKRDPEPERDPQVLNFRETLRKKGIPFIPLYQGIEFQVGLEDEQ
                     KGNLEAALEDMGLLDALIIPKRFQQKALELVKDGSDKVLCPSPKLMTTNLTQFLRPCR
                     VDGQDIALEDIDDALTSIMAYRDDQGFYIEDDGSYGMGIAAGKARTENVSKYIGAESR
                     KRYRLQMIENLNERLDELGTAMDGIRHRIDDLNNNMTDLEGDRGDFPNVEDLNTALEL
                     WHEAVRELKFLESKLLEHDILIGKQLKIVQERKAETYQKTKGLDLAPTLPAFINAEEG
                     MSRYRDLLHQLDRNHHSLVSERRMTDTKDEQYRETQDDVDILKGELNQLGDKIRACRR
                     MIEELEDLRSKPEFKEMQQEINRCIERIKEIPKEIRRAIDRSGRLQQAHITYKERLGA
                     MEEDQGLIIKKTGLLYEGFIQEYNLGFTPLEENLKEGRTMEEFPGIILKEWKQWADRW
                     GMDKALMGERLQRATYEALPELTEYGIALSTAFDASAGEDFDLIGIRAEFRRFVLTAR
                     LEGRQVSIYELDRWLGQEIDIHDNLLRDTDRQLFEEIIMQTVGRKIRAKIYRAEEWVK
                     NMNRLMGERDASNGLAFYLQWKPKVAQNEDELDTGDLVNILRTDANILRVEDFNRVTE
                     HFRSRVDRAKRMLDNNAHGQTFHNIIREILDYREWFEFVLYFRKEGENRRELTNRAFD
                     RMSGGEKAMAMYIPLFSSVYSRYESGGEQAPRLISLDEAFAGVDEKNIRDMFQLTEEL
                     EFNYIINSQILWGDYDTVGRLSICELLRPNNADFVSVIRYLWDGECKRIILDDQRDEV
                     AATREWTGQNG"
     gene            7376..>7654
                     /locus_tag="VFD89_01050"
     CDS             7376..>7654
                     /locus_tag="VFD89_01050"
                     /inference="COORDINATES: protein motif:HMM:NF023224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02679 domain-containing protein"
                     /protein_id="HZJ56812.1"
                     /translation="MDRRVKGREDLIYFKDNPGYRRIFNGIYKKYRSLGRLGGTITLR
                     GLTRDEKNVLSSHLRRDYSRKSSASFSVNAFVESLGHTRFNLYSLEEIL"
BASE COUNT         2338 a         1454 c         2101 g         1761 t
ORIGIN      
        1 gttcataccc tgtctggatc ccctagatga agagccgggg ggatatccct attccttgac
       61 acccgaaagg tttaccactt ttttaaaaac cttgtttgat gcctggtatg acgatatcaa
      121 aaaaggcaat atgataagca taaggtattt tgataactta gtgggaatga ttatgggcta
      181 tccgccggaa aactgcggaa tgtccggggt atgccaggtt caatttgtgg tagaagctga
      241 cggtggggta tacccctgcg acttctatgt aattgatggg tggtatatgg gaaaccttaa
      301 gacccagggg ttcgatgaac tgcataaatc ccaggcggcc agggaatttg tagaggtctc
      361 taaatatatt gatcctaaat gcagggaatg tagatgggta aatctatgca ggggcggatg
      421 cagaaggaac agggaacctt ttatagatga aaagcccgca ctgaactatt tttgctcatc
      481 ctatatggac ttctttgaat acgctacccc tagattatac gaactagcca gagcctttgc
      541 cggcagatag gagggtatat atgtcaaagc aattaacctt ggacaagcct ttggtagagg
      601 caagctattt aactgcccct aatttcttta gatatcgatc catacttcgc tttttttatc
      661 tgcagcacca aagaatgaag tattggatgg ttagagatga agtctttagc cggctgcggg
      721 aaatggaggg tcttcgagat tacactctgg atcaatgcca gcaggatctt gacaccttgg
      781 tggattggaa gaaccttatg gctggccaag atgcgggaaa ggttcaaacc attgaggagt
      841 ttaaaaatcg cagattcaga taccaactga ccccatatac catagagatt gagcgactga
      901 tggtcaggct cgaatccact tccggtatag ggggctccct tgaggctaac ctctttgaaa
      961 gaatcagcaa caggatccgg gaaatcccgg ggatcaaaga ggaaagcccc tcaagggtat
     1021 atggctggtg gcaggatctg gaatcggatt ttcaaaggct caaccagaat gccaccgact
     1081 atatagccag gttggaaggg ggcgaagctg aggagcttat gaacaccttg gcttttttga
     1141 catataagga tgggattatc gagtatctcc gtacctttat tcgcgaactg cagcgttatg
     1201 ggcccattat agaaggggat cttcgccaag tatccgaaga cacaacgggg aaacttctgg
     1261 atctggtatc aagccaccat atgaccattc cccgtatgga gcaggacttt accgaggacg
     1321 aataccggga ggaaatacgc agcagatggc tcaatttggc cctatggttt gtgggagaag
     1381 gcgacgaaga aagcgaatcc tacaggctcc tagatatcac caacggaatc atacgaaaga
     1441 tcacccgtta cgcctatcgc atagccgaaa gacaaaaccg ctctatcagc cggcaggggg
     1501 aatatatgca cctttgtaac ctctttgccg attgtcgtag cattgacgac gccaattcat
     1561 tgtctgcggc agtattcggt gctttttata cccgccacgt ccacgggagc ttcgaaaggg
     1621 agacggagag tatatcctca ggaatatggg aggaatatcc cttcgaggtc caattaaaac
     1681 ccaggacaag gcagtatagg gaaaggtccc atacgggaag catcgtcagc cgcgagacgg
     1741 aaaagaagag ggcgatggag gagtatcttg agagaatgag ggaggaacag gagatcttgg
     1801 cccaatatat agagggaaac cggctctcct ttataaactt gcctgagatt gagcccttcg
     1861 tcaggaatac tctgctccgc tgggtaggga gggccatggc caataaggaa agaatgggca
     1921 agacggaaga tggcagaacc tatagggtgc atatagataa gaagagcccc aggatgaccc
     1981 ttagatgtca ggatgggaat cttgagatgc cccccatgat catggaattt ttaagctgag
     2041 gtgaaataat agcgtggaag aatcggtgtt gattgaggat attatgacgc tgctggaaaa
     2101 tttctggatt cttcgggaaa aggacgatga aacctattac aggttaaagg ataatgaggc
     2161 aaggctgaag ccttttttgg aggagaagct ggggtatagg atgcatataa acccctatct
     2221 tataaagctg gataagatac cgggggaggc cgagacttgg atggggattg aatcctttaa
     2281 gactcccatg gattattgct tcctgttatt gcttctgtca tttcttgagg ataagggcct
     2341 tgaagaacag tttgttctgt cggatatcac ggagtatatt caaagcatgt accctgagca
     2401 agatacaata gattggacca tattccaaca caggcgttcc ttggttagga ccctaggatt
     2461 tgccagggac atgggcatta tgaagataaa cgacggggac gaaggggaat tttccagaac
     2521 ccaagacgca gaggtgctat atgaaagcac cgggatctcc aggtattttt taaggccctt
     2581 tacccgggac attaccgaga tgaatgggat agatgagatc ttggggagcg aatgggtaga
     2641 tatggacgaa gagaggggta taatacgtcg gcatagggta tacagaaggc tggttctatc
     2701 tccagcggtc tattccgccg gaaacgatga ccaggacttt tactatatac gaaactatcg
     2761 caatactatc caaagggata tagaggagta tatgggggcc tggctccacg tccataggaa
     2821 cacagctctg gtaatggtca acgaaagcta tggccggggt gacaggtttc ctgcccataa
     2881 caatttatcg gatatcgcac tgcagctgtc tactgtttta aggcgacagg tagatgaagg
     2941 gaaacggacc cccggggcgg atggaagtat tgtaatgact caagtagaat ttcggggttt
     3001 gctccaagat tgtcataggg aattttccca tggttggtat aaatcctaca gggaaatgaa
     3061 tatggaaaag ctcatggatg aggttaagga atatatgatg agatggaaga tgctggggga
     3121 ggatcaaagg cttaagacca taaaaatcct gcccttggcg gtaaaattta gcggagaatt
     3181 tcccaaagga tatagagagg gtgataaaaa gagatgagca gatggatgct ccataggatg
     3241 ggattggtaa acttttggtt ctatgacgag gaggaatttg attttgccaa cgggaaactg
     3301 ctccttagag gttccaacgg ttccgggaaa tccgttacca tgcagagctt tatacccttg
     3361 gtgctggacg gaaataagag cccggagagg ctggacccct tcggttccag ggccagaaaa
     3421 ctggaggatt acctcttggg cgaggaaggt gctaggagcc acgaagagcg aacgggatac
     3481 ctctatatgg agtttaggct cggggatctg gaacaatatt tgaccgttgg gatggggtta
     3541 agggcccgaa ggggcaggcc ggtagatttt tggggatttt gtataaccga cggacgccgc
     3601 ataggcaagg atattaccct ctatagggaa ggaatttccc cggaggacgg ccaagaatcg
     3661 cgtattcccc tatccaaaag tcagatgagg gagttagcag aagcgggcgg agtatttgtc
     3721 accggacaga aggactatat ggagatggta aatcgctata tatttggatt tgatacggtg
     3781 gaggaatacg atgaacttat taaacttctt atacagcttc ggagtcctaa attatcaaag
     3841 gactttaagc ccacggtcat ctacgaaata atgaattcgt cattgcctgc cctatcggat
     3901 gaggatttaa ggccacttac tgagaccata gagaatatgg accaaataaa gctgcatctt
     3961 gaccaattgc ttaaaaacca aaaggcggta tccaagctaa aagaggaata tcacaggtat
     4021 aataggctga cattgtttga gagggcgaga gcctttaacg aggggaagct aaagcaggat
     4081 gaaataaggg ataaaataaa ggcactagag gtcaatctag aaaaggatgc ccaggagata
     4141 gcactgctaa gtgagagtat tgcctcctta gaacatgagg aagaggccct gaggctaaag
     4201 gaaaggcaat acctggaaca tgatgcatat aaattggagc agcaatgggt ggaacaagga
     4261 cattcattga gggatatgga ggccaacaag acacaaaaac agggagtcct aaaggaaaaa
     4321 cagaagagag aatggagact gaaggcagag cttggggagt tggaggcaaa gatggcgacc
     4381 ctccaaaggc aagggcaaga gatcttaggc gaaatggacg atttagctga ggatatcaga
     4441 ttccatgacc atgagtatga cacgatagag ttcaatagga caggcagaaa acccttcgat
     4501 tttaccgctt ggaatcagga tgtccttctc tatagccaaa agctgaaaga gggcaggctc
     4561 gccttggagg ccgagattga agccaatcgt atttatgata atctcttaaa agagcaggat
     4621 accttggtgt ccggtaggga tgcggcatca cgcgaagaag aaaaggcttc ggaaatggtg
     4681 gatacatcca ggcagggcta tatggcttta ataaagtcat gggcggaaga gaataggata
     4741 tttaatatat tcggcgaaaa catattagcc ttcaatcaat tggtattaag atatggggaa
     4801 ggtaaggact taggagacct tcaagaattg ctaaacagcc tgtactaccc gcaatttcag
     4861 aaattggatc gggaaaaaat cagcttagag cacaccaggg atcagctggc aatagagata
     4921 aatgacttaa tggaagagct ttcgaaatgg aaaaacaaaa gggatccgga accggaaagg
     4981 gatccccagg tattgaattt tcgggagaca ttaagaaaga agggaatccc ttttatcccc
     5041 ctatatcaag gcatcgaatt ccaagtggga ttagaggatg aacaaaaggg taacctggaa
     5101 gccgccctgg aggatatggg actgttggat gccttgatta tacccaagag gtttcaacaa
     5161 aaggctttgg agctcgttaa agatggctct gacaaggtcc tatgcccgtc accaaagctt
     5221 atgaccacta atctgactca atttttaaga ccttgcaggg tagacggaca ggatattgca
     5281 ctggaggata tagacgatgc cctgacaagc attatggcct acagggatga tcaaggattt
     5341 tatatagaag atgacggcag ttatggtatg ggaatcgcag ccgggaaggc aaggaccgaa
     5401 aacgtatcca aatacatcgg tgcggagtcc cgaaaacgct accgtctcca gatgattgaa
     5461 aacctcaatg aacggctgga tgagctggga acagctatgg acgggatcag gcaccggatt
     5521 gatgatttga ataataacat gactgacctg gaaggggaca ggggggactt ccccaatgtg
     5581 gaggatttaa atactgcatt ggaactctgg catgaggccg tgagggagtt gaagtttttg
     5641 gaaagcaagc tgttggagca tgatatattg ataggaaagc agctaaaaat agtccaagaa
     5701 aggaaagccg aaacctacca aaagactaag ggacttgatc ttgcgcctac cctaccggct
     5761 tttataaacg ccgaagaggg gatgagccgc tatagggact tattacatca gctggacagg
     5821 aaccaccata gcttggtatc ggaacggagg atgacggata ctaaagatga gcaatacagg
     5881 gaaacccaag atgatgtaga tatcctaaag ggtgagttga atcagcttgg ggataaaatc
     5941 cgggcatgca ggcgtatgat agaggaattg gaggacctaa ggagcaaacc ggaatttaaa
     6001 gagatgcaac aggaaattaa tcgttgcatt gaaaggatca aggaaatccc caaggaaata
     6061 cgaagggcta ttgacagaag tggcaggctc cagcaagccc acattaccta taaggaaaga
     6121 ctgggggcca tggaagagga tcaaggactt atcataaaaa aaacagggct tttatacgag
     6181 ggttttattc aggaatacaa cctcggtttt actcccctag aagaaaacct taaagaggga
     6241 cgtacaatgg aagaattccc ggggataatc ctaaaggaat ggaaacaatg ggcggacaga
     6301 tgggggatgg acaaggccct gatgggtgaa aggcttcaaa gagccaccta tgaagcattg
     6361 cccgaactga ccgaatatgg cattgccttg agtacggcct ttgatgcctc cgccggagaa
     6421 gattttgacc ttataggcat cagagccgaa tttagacgct ttgttttaac tgccaggctg
     6481 gagggaagac aggtatccat ctacgagctg gacagatggc ttggacagga aattgacatt
     6541 catgacaacc tcctgaggga cacggacaga caactgtttg aagagattat catgcagacc
     6601 gtgggacgaa agatcagggc caagatatac cgggccgaag aatgggttaa aaacatgaac
     6661 cgcctgatgg gagagcggga tgcttccaac ggtttggcct tttatctgca atggaagccc
     6721 aaggttgcac aaaatgagga tgaacttgat accggagatc tcgtaaatat tctgagaacc
     6781 gatgccaaca tccttagggt ggaggacttt aacagggtaa cagaacattt tcgttcccga
     6841 gtagacaggg ccaagagaat gctggataac aacgcccatg gccagacctt ccataacata
     6901 attcgtgaaa tcctggatta ccgtgaatgg tttgaatttg ttctctactt cagaaaggaa
     6961 ggggagaaca gacgagagtt aaccaatcgc gcctttgatc gtatgagcgg cggggagaag
     7021 gctatggcca tgtatattcc actattttca tcggtgtatt cacgctacga atcgggggga
     7081 gagcaagccc ccagactaat atctctggat gaagcctttg ccggggttga tgagaaaaat
     7141 atcagggata tgtttcagct gactgaggag ctggagttta actatatcat aaattcccag
     7201 atcttatggg gggattacga taccgtaggc aggctttcca tatgtgaact cttgcgaccc
     7261 aataatgccg actttgtttc cgtaatccgc tatctatggg acggggagtg taaacggatt
     7321 atcttggacg accaaaggga tgaagtggct gcaacgcggg aatggacagg acaaaatgga
     7381 taggagagtt aagggtaggg aagacctaat atattttaag gataatcctg gttataggcg
     7441 gatctttaat gggatatata aaaagtatcg ctccttgggc aggctgggag gcactatcac
     7501 ccttcgaggc ctgacccggg atgaaaagaa tgttttaagc agccatctaa gaagggatta
     7561 ttcgcggaag tcctcggcca gcttttccgt aaatgccttt gtagaatccc tcgggcatac
     7621 aaggtttaat ttatattccc ttgaagaaat attg
//
LOCUS       DASRNX010000028         7987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_695160,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000028 DASRNX010000000
VERSION     DASRNX010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695160"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..1545)
                     /locus_tag="VFD89_01055"
     CDS             complement(274..1545)
                     /locus_tag="VFD89_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007087261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HZJ56813.1"
                     /translation="MLTREERIKWFQKDRFGMFIHWGIYAIPARGEWVRSREGITIED
                     YQPFFEEFDPVHYNPRKWARAAKEAGMKYAVMTAKHHDGFCLFDSHLTDYKATNTPAG
                     RDLIEEYVEAFRAEGLKVGFYYSIIDWHHPDFPAYGDRHHPMRDNENWKDREKDQDFS
                     RYIEYFHGQVKELVTNYGKIDIMWFDFSYDDMTGEKWEATKLVEMIRKIQPGIIIDNR
                     LTQHGDHDDPKPYAGDFASPEQIIPPEGIRNQKGEPIPWEACITMNDNWGYAAADKDF
                     KSPKQLIRALVECVSKGGNMLLNVGPDAKGQIPKESLKILAEIGEWMKQNSDSIYGCG
                     LADIPKPEWGRYTQNGNRLYAHIFDRGIGAIAVQGLKGKVKRARLLIDKSEVNIDTPW
                     TSMHYGDATFINLPSTQLPDERDTVVEFELE"
     gene            2064..2777
                     /locus_tag="VFD89_01060"
     CDS             2064..2777
                     /locus_tag="VFD89_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZJ56814.1"
                     /translation="MRTILITGADRGVGLGLTTVFLEAGWIVFAGQYMPQWKELEELY
                     HKYPETLHIVDLDVSKGESVQNCYDIVSDKVGHLDILVSNAGIVGSLGDIFDLQGLDK
                     GMNAFNVNTLGSFRMVQTFLPLIDREVSMKRLCFVSSEAGSISVCHRKDGFTYPMSKT
                     ALNMGVKLLFKELRPKGFTFRLYHPGWVRSYMSGKKNTSGTYEPIETARSAFEQFTSD
                     SPREDVLFLHDNENITWPF"
     gene            2789..3361
                     /locus_tag="VFD89_01065"
     CDS             2789..3361
                     /locus_tag="VFD89_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56815.1"
                     /translation="MKRIIISGSKGKFSESLTRYFESKGYRIWQPGDPLDMFVYIIEP
                     AGTEDESINMDFDYDDLLQKHENIALDMLKETSNSLNLFRDMDKARLCYVNSVRSSVN
                     RAADFSGGYDRMISAACNMAIRIMFNRLRPLGYTFRVFAAQDFKSPKESSYAAEYFMT
                     DRSLEEEEPLIRSDENRLVMRDMYEEEIPW"
     gene            3456..4106
                     /locus_tag="VFD89_01070"
     CDS             3456..4106
                     /locus_tag="VFD89_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZJ56816.1"
                     /translation="MLNVLVLCDDYWHPGEVIEMGIKPLEGPEFHFDFVKAAKDILTP
                     EMIAEYQVIVCCKGNSINAANRSPWLTDDVTEVTTKEFEDYVKNGGGFLAVHSGNTSR
                     EGDEYTEFVGNYFVGHPPRCDVNVNITGEHPIVDGVKDFTIRDEHYNIHVTADDITEL
                     FTTTSETGGTQMGGYTREMGQGRLCVLTPGHILDVWKHDEFKKIFLNAIRWCGKKK"
     gene            4800..5600
                     /locus_tag="VFD89_01075"
     CDS             4800..5600
                     /locus_tag="VFD89_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZJ56817.1"
                     /translation="MGKYRYGVELYSVKNALGKDMPATLRRVKEMGYEAVEFFGTFQH
                     PAKEVKQALDDTGLVCCGWHTPWQYVVDDALASTVEYFHAIENPYVIIPGLPREMTGS
                     YEQWLRNAQLFNDVARKLSDHGLRLGYHNHAGELHPFEDAEGCPFTVLFDNTDPSIIV
                     QMDNGHVLNGRGMDMLTLLKRYPGRYTTVHLKPYSLEKGSENPNDGYMTMIGEDDVPW
                     AEFMTMCREIGGTKWYIVEYESIELYPEFEGVEKCLQALKGMEAEGKF"
     gene            5819..6655
                     /locus_tag="VFD89_01080"
     CDS             5819..6655
                     /locus_tag="VFD89_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56818.1"
                     /translation="MKKVGFIDYFLDEWHANNYPDMIKNATSGEMIVTHAYGKIDVPG
                     KRDNRSWCSEMGILYCETIEQVVEETDYIIVLSPDNPEMHEELCQIPLKSGKPVYVDK
                     TFAPTKQIARGLFDLAREYNTPLFSTSALRFSKEVMELEKGDIDFIASVGPGEIDNYL
                     IHQVEPMVMLMDSPATKLMYTGTSRTPCIVIDFENDKRGVISVLVGDCNFALSIRYKD
                     GKVCVIPEATEFFDRFIVNMVEFFKSGKSSVPGSQTIEIIDIIEKANFAQKRPDTWID
                     LK"
     gene            6680..7741
                     /locus_tag="VFD89_01085"
     CDS             6680..7741
                     /locus_tag="VFD89_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ56819.1"
                     /translation="MGKIKVAVIGCGTIANSAHIPSYLANGQVEIKYFCDIIPKRAQG
                     AVEKYGCGSAVVDYRDILDDDEIVAVSVCTPNNVHADISIDFMRAGKHVLCEKPAART
                     YEEALRMQKVQNETGKILNIGVVNRFNNGVNVIKNMIDEGELGEIYHVYASFRAHRSI
                     PGLGGAFTTKEIAGGGALIDWGVHFLDIVMYCCQDPKPLTVTGEAFSKLGRDMEGYVF
                     TEMWAGPPQYDGTYDVEDSITGMIRTEGPVITLNGAWAQNIGENEMFIDFMGTKGGIR
                     LSYGADFKFYTVRGGALLECTPKYISKDHFQNEIDAFVSCIQTGEKLPSHIDIVIVTA
                     QIMQALYDSSEEHREIKLG"
BASE COUNT         2325 a         1535 c         1941 g         2186 t
ORIGIN      
        1 gattagtaaa acaggtgggg ggtatatcat atattccccc gcttcgctac aacccgtgcg
       61 ttgtaattga caatgaaatt atttccgatg ttataaaaat cacgtttatt ataggaaacc
      121 atgtttatgg aaatgaagat aagtaaagca tgctgtcagc acgcttcact tatctttaaa
      181 tatactgttc caaattcaat tacacttccg ttatttaagt tccaatttaa ttctgatttt
      241 gtttcccatc ctgctattaa aattccatcc ctactattcc aattcaaatt ccactactgt
      301 atccctttca tcgggcaatt gggtactggg gagatttatg aacgtagcat cgccgtagtg
      361 catcgaggtc cagggagtgt ctatattgac ttcggatttg tcgattaaaa ggcgtgccct
      421 ctttacctta cccttgagac cctgaactgc tatggcgccg atgcccctgt caaatatatg
      481 agcatagagc ctgtttccgt tttgtgtata tctgccccat tcgggctttg gaatatccgc
      541 aagtccgcat ccatagatgc tatcgctatt ttgtttcatc cattccccga tttcggccaa
      601 gatctttaaa gattctttgg ggatctgtcc ctttgcatca ggacctacat tgaggagcat
      661 attgcccccc ttgctgacgc attccaccaa tgcacggatg agctgtttgg gagatttaaa
      721 gtccttatca gccgcggcat atccccagtt gtcgttcatg gttatgcaag cttcccaggg
      781 aataggttct cccttttgat ttctgattcc ctccgggggt atgatctgct ccggcgatgc
      841 aaaatctccg gcatagggtt ttgggtcgtc atggtcgcca tgctgagtca gtcgattgtc
      901 tatgatgatg ccgggctgaa ttttccgtat catttcaact aacttggtag cctcccactt
      961 ttcgccggtc atatcatcat acgaaaagtc gaaccacatt atatcgattt ttccgtaatt
     1021 cgtgaccaac tccttgacct ggccatggaa gtattctatg tacctggaaa aatcctgatc
     1081 cttctcccta tccttccaat tttcattgtc cctcatagga tgatgacggt caccgtatgc
     1141 ggggaaatcg ggatgatgcc aatcgattat ggaataataa aagcccacct taagaccctc
     1201 ggcacggaaa gcctcaacat attcctcaat aagatccctt ccggcggggg tgttggttgc
     1261 cttgtagtcg gtaagatggc tgtcaaagag acaaaaaccg tcatggtgtt tggcggtcat
     1321 aacggcatat ttcatacccg cctccttggc agccctggcc catttcctgg ggttataatg
     1381 gacaggatcg aattcctcga aaaaaggctg atagtcttcg atggttatcc cttcccggct
     1441 gcgtacccat tcgcccctgg cggggatggc gtatataccc caatggataa acataccgaa
     1501 ccggtccttt tgaaaccatt taatccgctc ttccctagtc aacatttcat tcctccttat
     1561 gtgttttttg tttcccctgc aaaagcaact ttttgtaaca agatttgaat ttcatagaaa
     1621 gggggcttgt ttcggaagca attactttag taattacatc gcatgaaatt ataggcaata
     1681 catttgttta taagaatcac aaaaaagcta agtatagaag aaaggttacg tccatatcat
     1741 actaaagaat attgccaagt tcaagagaac ctttggcatt caatcattat tttcaattat
     1801 attaatctat ggtgggacaa atttcgaaag gctatggtat aatataactg tggatgatat
     1861 catggattta tacattccag tatttttgtg aatccggggc aagggaaaga aaatataggg
     1921 gtaggggtag gcacctatag cttattggcg gtcgacaatt ccggaaagat gagcctttta
     1981 gatgttatcc ataggattat taataccagg gatctgatgt tttgaatgta gctaaagata
     2041 tagaagaagg agtggtataa aaaatgagga caatacttat tacaggcgca gacagagggg
     2101 tgggtttagg gctgacaaca gtattccttg aggctggatg gatcgtgttt gccgggcaat
     2161 acatgcccca atggaaggag cttgaagagc tgtaccataa gtatccggag acattacata
     2221 tagtggattt ggatgtatca aaaggagaat cggtgcaaaa ctgctatgac attgtatcgg
     2281 ataaagtggg acatctcgac attcttgtct ccaatgccgg aatagttggg agccttgggg
     2341 atatatttga tctacagggc cttgataaag gtatgaatgc gtttaatgtg aacaccttag
     2401 gttctttccg tatggtacag acttttctgc cgttaattga cagagaagtt agcatgaaga
     2461 ggctgtgctt cgtatcatcg gaggccggga gcataagcgt ctgccataga aaggacggat
     2521 ttacctatcc tatgtcaaag acggctctta atatgggtgt aaaattatta tttaaggaat
     2581 tgaggccaaa gggcttcacc tttagattat atcatccggg ttgggttaga tcatatatgt
     2641 ccggtaaaaa gaatacctcg ggaacatatg agcctattga aacagcgaga tcagcttttg
     2701 aacagtttac aagcgatagc ccacgcgagg atgtgttgtt tttgcacgat aatgaaaata
     2761 ttacttggcc gttttaggag gtttgacaat gaaaaggata attatttcag gatctaaggg
     2821 taagttttct gaatccttga cccgatactt tgaaagcaag gggtatcgta tatggcagcc
     2881 cggtgatcct ttggatatgt ttgtatatat aattgaacct gcaggcacgg aggacgaaag
     2941 cataaatatg gattttgact acgatgatct gctgcaaaaa catgagaata ttgctttgga
     3001 catgctaaag gagacctcta attccctgaa cctcttcagg gatatggata aagcccggct
     3061 gtgctatgtg aattccgtga gatccagtgt taacagggcg gccgatttta gtggtggata
     3121 tgacaggatg atcagcgcag cctgcaatat ggccataagg ataatgttca acaggctaag
     3181 gcccttagga tataccttta gggtgtttgc cgcccaagat tttaaaagcc caaaggaatc
     3241 aagctatgcg gcggagtatt tcatgacgga caggagcctt gaggaggaag aaccccttat
     3301 aaggtccgat gaaaaccgtc tggtcatgag ggatatgtac gaggaagaaa tcccgtggta
     3361 aaagatgatg acacccttta agcggaacag agtcctaata atgaaggaga tatttacagg
     3421 taatcaaaga ttatacgaga agggagactt taattatgtt aaacgttttg gtattatgcg
     3481 atgattattg gcatccgggc gaggtaatag agatggggat taagcccctt gaagggcctg
     3541 aatttcattt tgattttgta aaggctgcta aggatatact gacacccgag atgatagctg
     3601 agtatcaggt aatcgtttgt tgcaaaggga atagtatcaa tgccgctaat aggagcccat
     3661 ggctcaccga tgacgtaacg gaggtaacca caaaggagtt tgaggattat gtgaaaaatg
     3721 gtggcggatt tttagcggtc cattccggca acacctcaag ggaaggagac gaatatacag
     3781 agtttgtcgg taattatttt gtagggcatc cccctagatg cgatgtaaat gtaaatataa
     3841 caggggaaca tccaatcgta gatggcgtaa aggattttac cataagggac gagcactata
     3901 atatacacgt gaccgccgat gacataaccg agctttttac aactacatca gagacaggag
     3961 gcactcagat gggcggttat acccgggaga tgggccaggg acgactttgc gtattgacgc
     4021 ctgggcatat tctggatgtt tggaagcatg acgagtttaa aaaaatattc ctaaacgcaa
     4081 tacggtggtg cggaaagaaa aaataacgat atacatatgg atcggtatca tctgaatcgg
     4141 tatccccgtt tttgggataa tatatagtca gggcgccaag gctctatttc gcgaacaatc
     4201 cataccatat atagttttaa taccaatcgt ccggactaca aacggttgta taaaattggg
     4261 ccatggtctt ttgacccctg aattaatata tgcggttatc aaaggcgaaa gtgcctgcca
     4321 taacgagatg gcgggcactt ttgtatgaca ctgcgtctcc tgcgtattga ttaattttct
     4381 tttcctattg atttccatac acatatcaac taaactagat attgggaagc ttcatttaag
     4441 ctagatattg ggaggcttga ttgtccggta ttagttaatc ggctatattc cggcaaggtt
     4501 aaagtgcttg acccataaga aacatcacct ttttcatcga acgataacac cttatacggg
     4561 atatgtaata aatttcacat ctatacagag tatttgcttt ttatagaata cggatgttat
     4621 ttcaaatatg gtattcataa agcttaggaa tagttttggt acttatgatt aacaatcaag
     4681 gagtatgatg gatatggcat taaccgggcc ggatacccag gcaggactaa gagcgatggg
     4741 gctggttatc ttgctattgc ggaagaatcg aagcttttta ataaagagga ggtagagaca
     4801 tgggaaagta taggtacggg gtggagctct attccgtaaa aaatgcattg ggaaaggaca
     4861 tgcccgccac acttcggcga gtaaaggaaa tgggatatga ggcagtagag tttttcggga
     4921 catttcagca ccccgcaaag gaggtaaaac aggcattaga tgataccgga ttggtctgtt
     4981 gcggttggca cacgccatgg caatacgttg tagacgatgc tctggcaagt accgttgaat
     5041 attttcacgc tatcgaaaat ccttatgtaa taatacccgg cctgccaagg gagatgaccg
     5101 gatcctatga gcaatggctc cgtaatgccc agttgtttaa tgatgtagcc cgcaagctat
     5161 cggatcatgg gttacgcctt ggatatcata accacgcagg ggaacttcat cctttcgaag
     5221 atgccgaggg atgtcccttt accgtattgt tcgataacac agacccgtca atcatcgtac
     5281 agatggataa tggccatgtg ttaaacggca ggggtatgga catgctgacg ttattaaagc
     5341 gctatcctgg gcgctacact actgtccatt taaagcccta tagtctggaa aagggctcgg
     5401 aaaaccccaa cgacggatat atgaccatga tcggtgagga tgatgtgcct tgggctgaat
     5461 ttatgaccat gtgccgagag ataggcggga cgaaatggta tatcgtcgaa tatgagagca
     5521 ttgaactcta tccggaattt gagggtgtag aaaagtgcct gcaggctctt aaaggcatgg
     5581 aagcggaagg gaagttttaa gtcatcaata ctttgtcttg atgatcatat tgcggccttt
     5641 agcggtgagc ccggacagat atgtcataaa aacccatcat ttacaaaaac ggttagacta
     5701 tatagcctta tattaaggca atgtgttttt ggacatatga attaggggtg atgtttacag
     5761 tgtctgatct ctaacacgga atattggttg taaaatatac ggaaaggagc aattaagcat
     5821 gaagaaggta ggatttatcg actattttct tgatgaatgg catgcaaata actatccgga
     5881 tatgataaaa aatgctactt cgggggaaat gatagtcaca catgcctatg gcaaaattga
     5941 tgtgccgggg aaaagggata ataggtcctg gtgcagcgaa atgggtatcc tttattgcga
     6001 gaccattgaa caggtagtgg aagagacgga ttacataatt gttttatcgc cggataatcc
     6061 cgagatgcat gaggagttat gccaaatacc cctaaaatct ggcaaacccg tctatgtcga
     6121 caagaccttt gccccgacaa agcagatagc aagggggcta ttcgatctgg caagggagta
     6181 taacacccca ttattttcga catcggccct gaggttttcc aaagaggtta tggaacttga
     6241 aaaaggggat attgatttta ttgcaagcgt cgggcccggg gagatagaca actacctaat
     6301 ccaccaggtc gagcccatgg taatgctgat ggacagcccc gccacaaaac ttatgtatac
     6361 cgggacatcc cggaccccct gtatagtgat agactttgag aatgataaac ggggcgtaat
     6421 atcggtgtta gtaggagatt gtaattttgc attgtctatt agatataagg acggtaaggt
     6481 ttgtgtaata cccgaggcca cggaattctt tgacaggttc atcgtcaata tggtggaatt
     6541 ctttaaatcc ggtaaaagca gtgtacccgg ctcacaaacc attgagatca ttgacataat
     6601 cgaaaaggcc aattttgccc aaaaacgtcc tgacacctgg attgatttaa aataatatta
     6661 tatatggggg atatgagata tgggaaaaat taaggtagca gtaataggat gcggtactat
     6721 cgccaatagt gcccatatac cgtcgtactt agccaacggt caagtggaga ttaagtattt
     6781 ttgcgatatc ataccgaaaa gggcccaggg agcagttgag aaatatggat gcggcagcgc
     6841 tgtagtagat tatcgagaca tattagacga tgatgagatc gtcgcagtat ccgtctgtac
     6901 gccaaacaat gtacacgcgg acatttccat agacttcatg cgggcaggca agcatgtatt
     6961 gtgtgagaaa cccgctgcca ggacatacga ggaagcctta aggatgcaaa aggtccagaa
     7021 cgaaacagga aaaatattaa atatcggggt agtaaatagg ttcaacaacg gcgtgaatgt
     7081 aattaagaat atgatagacg agggcgagtt aggagaaata taccacgtat acgcaagttt
     7141 cagggctcat cgcagtattc ccggcctagg cggagcattt acaacaaagg agatagcggg
     7201 gggcggggcc ttaatcgact ggggcgtaca ttttctagat atcgtgatgt attgttgtca
     7261 ggatccaaaa cccctgacgg tgacggggga agccttttca aagctgggca gggacatgga
     7321 gggctatgta tttacggaga tgtgggccgg accgccccaa tacgacggaa cctatgacgt
     7381 tgaggactcc attacgggga tgataagaac tgagggccct gtgattacat tgaacggtgc
     7441 atgggcacaa aacattggcg agaatgagat gtttatagac tttatgggca caaaaggcgg
     7501 aatccgtcta tcctatgggg cagactttaa gttttatacg gtgaggggag gagccctgct
     7561 tgaatgcacc cccaaatata tatcgaagga tcacttccaa aacgaaattg atgcatttgt
     7621 aagttgtata cagaccggcg aaaagctgcc ttcacatatc gatattgtca tagttacagc
     7681 ccagattatg caggcattgt atgattcgtc cgaagagcac cgggagatta aattgggata
     7741 ggggtatacc catctgaatg gaataggagt atcctcatct gaaactgtat atattggaca
     7801 acacgacaac gtggatttgg tttgagaaaa ttccggtcga taacccacca cctaccatac
     7861 gggcgttcac aacggtacag aacccggtaa tatgggtaag gggcagacga tggcttcgtc
     7921 tgctcattac ccattcttgt tctacacata ttgaaaatac ggtatattat agtgtataat
     7981 gttatct
//
LOCUS       DASRNX010000029         6794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_535943,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000029 DASRNX010000000
VERSION     DASRNX010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535943"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..831
                     /locus_tag="VFD89_01090"
     CDS             283..831
                     /locus_tag="VFD89_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZJ56820.1"
                     /translation="MDDFALKKRIRYGTVMVDLNTHSIIDMIESREQEKVAEWLKSYP
                     NIQVVSRDGSVTYHNSITEAHPMAVQVSDRFHLFKNLTDYAVEYLKKHLSKVIKIVTE
                     PKDKVKSAVLGIDKGNENRKLTLEEKYHKVLLLQAEQRSQTKYAAMLIWMLGVTKNLC
                     LLLIMTGRRCLKRWLALDMKIE"
     gene            complement(974..1783)
                     /gene="istB"
                     /locus_tag="VFD89_01095"
     CDS             complement(974..1783)
                     /gene="istB"
                     /locus_tag="VFD89_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZJ56821.1"
                     /translation="MINQSTIDKLHQMRMATMADTFTSQMRDTTYDSLTFEERFGMIV
                     DAEWARRRSTKLQKLIRTAGFRYPNACMEDIEYHPDRKLNKPQLLQLSTCRYIREGHH
                     IILGGASGNGKTFLACALGVAACRNFMTVRYIRLPELLNDLVVARGEGTYKKLIKTYQ
                     KVDLLIFDEWLLKTLTTEQAMDLFEIIEARTHKGAMIFCAQFDPRGWYERIGTSEDGT
                     VSEAIIDRIKHNAYEILIDGEESMRERHGLKNSSPPTLSNIDQIDIPPNAE"
     gene            complement(1776..3320)
                     /gene="istA"
                     /locus_tag="VFD89_01100"
     CDS             complement(1776..3320)
                     /gene="istA"
                     /locus_tag="VFD89_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZJ56822.1"
                     /translation="MVNYREILRLRSLGYSQRQVAASVHSSRRTISEVYELADTNGLS
                     WPLPSDITNTDVRDILYPERAVESGRKMPDCSYLHKELAKPGVTLTLLWSEYCEKCHI
                     EGLIPYQYTQFCEHYRAFASKTKATMRIKRKPGELLEVDWAGSTLVVVDEITGEIIPA
                     YVFVAALPCSLYGYAEAFPSMKMENWINAHIHMYQFFGGATRIVVPDNLKVGVIKHTR
                     NEIVLNKVYQEMAEHYGTAIIPARPVSPKDKPSVEGTVGVISTWVIAALRNEKFFSFS
                     ELNDAVKVKIKEFNSKPFQKKRGSRLSAFEEEEKPFLLPLPTSPYEMAVWSTATIQSD
                     YLITVGKCKYSIPYEFIGHTVDIRYTSKTIEVFFQNNRIASHVRMYGIYDPQILPEHM
                     PDNHRKYLAWNKETFLDWAETVGPSTSIVMKALLASHKVEKLSYKSCGALMKLAGRYS
                     VARIEEACGRALSYTPNPNLKNIQMILKTGQDKVKPEKETTKSQPSGRYGFTRGAEYF
                     GGGHND"
     gene            complement(3954..4808)
                     /locus_tag="VFD89_01105"
     CDS             complement(3954..4808)
                     /locus_tag="VFD89_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56823.1"
                     /translation="MHFGYFTDYDLEFLDRHINWTDILKSYAISHEELNTIIKESKND
                     IYHQFENHLDGKHFNGEMFGISSLDQFYRTNDRYESPGINIVTYSTDYFTMNSMAEIL
                     KRLRARRPEVRMEIGGLSYFRNSIGVNVLLILPSTNEIVMVKRSKHSSYTEEKRLIYP
                     STVETLSIADETSKINIDLCVYRGIEEELGLTIDELDTKSLINYAMFFENNYFQDNFV
                     LSISADESITLQNIRDRLAKDRMLEIEEIDSIPNNKRAIDRYIARNKNNMQFQTIYCL
                     EIYKKMTF"
     gene            complement(4775..5053)
                     /locus_tag="VFD89_01110"
     CDS             complement(4775..5053)
                     /locus_tag="VFD89_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56824.1"
                     /translation="MIDRINNLLKNDWIFNILFIFLGMFIENIWQRLRILNRRLIGRK
                     KKEDFIRALDRDLGITTLAYGYHPLLGLISLQARKPSYCILAILLIMT"
     gene            complement(5344..6744)
                     /locus_tag="VFD89_01115"
     CDS             complement(5344..6744)
                     /locus_tag="VFD89_01115"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZJ56825.1"
                     /translation="MSKIAVIGTGYVGLVTGTCLSDFGHNIICVDNDREKIDKLQKGI
                     IPIYEPGLAPIVERNVYYKRLLFTRDIKEAVEECDVIFIAVGTPPADDGSADLQYVEA
                     VARDIARHMNRYKVIVNKSTVPIGTGQRVKGWVTEGLLDRGVSHDFDVVSNPEFLREG
                     SAVHDFTHPDRVVIGAESKKAMDIMKNVYSVLYLNETPFVETNIETSEMIKYSSNAFL
                     AMKITFINEVANLCEKVGANVQDVARAMGRDGRISPKFLNPGPGYGGSCFPKDTLALA
                     QIGKEYGAPVTLIEKTVEANELQKKIAAQKVEDVLGDLSGRTLSILGLSFKPNTDDMR
                     EAPSITILNELAKKGADFRVYDPIAYKEAKWRLKNLEDRVTYCQNEYEAMEGSDALII
                     ITEWNQFRNLNLKRVKELLKEPYFFDFRNIYRRDTLESQGFFYTGMGQGAKGTFSLSS
                     GKEEPPALSEVAAGKE"
BASE COUNT         1787 a         1500 c         1429 g         2078 t
ORIGIN      
        1 gtaaaaaggt gatagttatt attaagaatc ggaagatgtt ttgtaataat ccccaatgca
       61 atcatacgac ctttgctgaa agattttcct gactatcggt taaatctaag aaaaccaagc
      121 ggctcaaaga cgaaatagta catctatcac tgaattgcag ctctgttgca gctgcaaggt
      181 ttttaaatga aaacacggtt gctgttggta aaagtaccat atgtaatctt ttaaaaaaaa
      241 ggtagactta tagttgataa ggaagccgta actgccgtat gtatagatga ttttgcatta
      301 aagaaaagaa taagatatgg gacagtaatg gttgatttaa atacccattc tattatagac
      361 atgatcgaat ccagagagca ggaaaaggtt gcagaatggc taaaatcgta cccaaatatc
      421 caggtagtct caagggatgg ttctgtaaca tatcataact ctattactga ggcgcatcct
      481 atggctgtgc aagtaagtga tcgttttcat ttatttaaga accttacaga ttatgctgtt
      541 gaatatttaa aaaagcatct aagcaaagtc attaagatag ttacagaacc taaagataag
      601 gtcaaatccg cagtattagg aatagacaaa ggtaatgaaa acagaaaact aacattagag
      661 gagaaatacc ataaggtact tttattgcaa gcggaacaaa ggagccaaac caaatatgcc
      721 gcaatgttaa tatggatgtt aggtgttaca aaaaacttat gtctgcttct gataatgaca
      781 ggaaggagat gtttaaaacg gtggctggcg ctagacatga agatagagtg aatattacgg
      841 ttcgataata ccaccttaac ggcgaaagtg aaccaccatc acgggctaag ggagccacta
      901 atatcggcat gcaggaccac ccaatcggat ggccctggca taccaattga taaatgtacc
      961 ctaacaccag atattattct gcatttggcg gaatatctat ttggtcgatg ttacttaagg
     1021 ttggtgggct gctgtttttg agtccatgac gctcacgcat cgattcctct ccatcaatta
     1081 ggatttcata ggcattatgc tttattcgat caatgatggc ttcggatacg gtaccatctt
     1141 ctgatgtacc aattcgctca taccagcctc ttggatcaaa ctgtgcacaa aaaatcatgg
     1201 ctcctttatg tgttctggct tcgataatct caaacaaatc cattgcctgt tctgtagtca
     1261 gtgtttttaa gagccactcg tcaaatatca gtaggtccac cttctgatag gttttgataa
     1321 gctttttgta agtgccttca ccacgtgcaa ctactagatc atttagtaat tcaggaaggc
     1381 gtatgtagcg gacggtcata aagtttcggc aagctgcgac gcctaatgcg catgctaaaa
     1441 aggtctttcc attccctgac gctcctccca gtatgatatg gtgcccttcg cggatgtacc
     1501 ggcacgttga tagctgaagt agctgtggtt tattcagctt tctgtcagga tgatattcga
     1561 tatcttccat gcaggcattt ggatatctga acccagcagt gcgaataagt ttttgaagct
     1621 ttgttgaacg ccgccttgcc cattccgcat ccacaatcat accgaaacgc tcttcaaatg
     1681 taagggagtc ataagtggta tctctcattt gtgaggtgaa tgtatcggcc attgtagcca
     1741 tacgcatctg gtgtagttta tcgatagtgg actgattaat cattatggcc acctccaaaa
     1801 tactcagctc cccttgtaaa accatagcgt ccacttggct gagattttgt tgtttccttc
     1861 tccggcttta ctttatcctg tccagtcttt agtatcattt ggatgttctt taagttgggg
     1921 tttggggtat aggaaagcgc tcggccacag gcttcttcaa tacgggccac ggaataacgg
     1981 ccagcaagct tcataagagc tccgcaggat ttgtagctta gcttttctac tttatgggag
     2041 gctaataagg ctttcatcac tattgaagtt gatgggccaa ctgtttctgc ccaatcaaga
     2101 aaggtttctt tgttccaagc tagatacttt cggtgattgt cgggcatatg ttccgggaga
     2161 atttgtgggt catagattcc atacattctt acgtgtgagg ctatccggtt attctggaag
     2221 aatacttcta tcgttttgct cgtataacgg atatccacgg tatgcccaat gaattcatag
     2281 ggtattgaat acttgcattt acccacagtt atgagataat cggattgtat tgtggcagtt
     2341 gaccagacag ccatctcata aggggaagta ggcaaaggca gaagaaaggg cttttcctct
     2401 tcttcaaagg cggaaagcct gcttcctcgc tttttctgaa atggctttga attaaattct
     2461 tttatcttta cttttaccgc atcatttagc tcagagaatg aaaaaaactt ctcgtttcgg
     2521 agtgccgcaa tgacccacgt ggaaatgacc ccaacggttc cttctacact gggcttatcc
     2581 tttggcgaga ccgggcgcgc aggtatgatt gcagttccgt agtgttcagc catttcctga
     2641 tacaccttgt ttagaacgat ttcgtttcta gtgtgcttaa ttacaccaac cttgaggtta
     2701 tcaggaacta caatgcgagt tgctccacca aagaattggt acatatgaat atgggcattg
     2761 atccaattct ccattttcat agatgggaag gcttcggcat agccataaag gctgcaaggg
     2821 agtgcagcaa caaagacgta tgccggaata atttctcccg ttatttcatc aacaacgact
     2881 aaggtgcttc ctgcccaatc aacttcaaga agctcgcccg gttttctctt aatacgcata
     2941 gtagcctttg tcttacttgc aaaggcgcga taatgctcac aaaactgcgt atattggtat
     3001 ggaattagcc cctcaatgtg gcacttctcg cagtattcgc tccaaagcaa ggtaagggta
     3061 acgccgggtt tagccagttc cttatgaaga tagctgcaat cgggcatctt tcttcctgat
     3121 tcaactgccc tctcagggta tagaatatct ctcacatccg tattcgttat atcggatgga
     3181 agtggccatg ataggccgtt tgtatcagcg agttcatata cttcgctaat ggtcctacgt
     3241 gagctatgga cactggcagc aacttgacgt tggctatatc ctaggctgcg tagtcttaga
     3301 atttcacgat aatttaccat aattatcgcc tccataacag aatttgcaca attgctgtgc
     3361 ataaattcta ttatttccct aaatatggcg gtggtatcca tagcgtgaaa gtggttttat
     3421 tttaccgtga gagtggttcc aatttaccgt tacagtggta ttactagacc gaaatactca
     3481 gtctataccc tgtattgcca taaatacccc aatataaggg cctttacccg ccttatcgcc
     3541 ttataatccg tagggcactt aactacaaag ttgatgaaat agcactacgt catgcttcag
     3601 tagtatttat cactgcaggt catgggttcg gtagaattag acgttagggt atataaattg
     3661 ccgggtgtac tcatataaat gctaagctat tgaggagctg atattttaaa tacctccaaa
     3721 tgaagttgtc tatagatctg taaataaggc cgtagatcga tccccttatc ccttgcccga
     3781 tcatttatga aatacgccag atatcagagt gcgtttcact taatatcttg agtgcaagag
     3841 gatgattcct tctgtgcctc gatatacaga acacagaagg accgtcagat tgtgttcgca
     3901 gccatacacc aaagggtgac aatttgggaa cgtccccttt tgtcacccta gcatcagaag
     3961 gtcatcttct tgtatatttc caagcaatag atcgtctgaa actgcatatt gtttttattc
     4021 ctggcaatgt atctgtctat ggcccgttta ttatttggta tggaatcgat ctcttcgatc
     4081 tcaagcatgc gatccttggc cagacgatcc cggatgtttt gaagggttat agactcgtct
     4141 gcagatatgc tgagtacgaa attatcctga aaatagttgt tttcgaagaa catagcataa
     4201 tttatcaagg atttggtatc tagctcatct atggtcaacc ccaattcctc ctctatcccg
     4261 cgatagacac agagatcaat atttatcttg ctcgtttcat cggctatgga tagggtttcc
     4321 acggtggatg gatatattag acgcttctcc tctgtgtagg aggagtgttt tgaacgcttt
     4381 accatcacaa tttcgtttgt ggatgggagt atcaacagta cattgacccc tatggagttt
     4441 ctaaaatatg aaagtccccc tatttccatc ctgacctcgg gccttcttgc ccttaaacgc
     4501 tttagtatct ctgccatgga gttcattgta aaatagtctg tggaatatgt tacgatattg
     4561 atcccgggtg attcgtatct gtcattggtt cgataaaatt ggtcaaggct tgaaatgcca
     4621 aacatctccc cgttgaaatg tttgccgtct aggtgatttt cgaattgatg gtagatatca
     4681 ttctttgatt ctttaattat cgtatttagt tcttcatggg atatggcata actttttaat
     4741 atatcggtcc aatttatatg tctatccagg aattctaagt cataatcagt aaaatagcca
     4801 aaatgcaata ggaaggtttc cttgcctgca atgatatcaa ccctagcaaa ggatggtatc
     4861 cataggctag ggtagtaata cccaggtccc tatccaaagc ccggataaag tcttcctttt
     4921 tcttgcgtcc gataaggcgt ctgtttaata tcctaagcct ttgccatatg ttctctataa
     4981 acatgcccaa gaagatgaag aggatattaa agatccaatc attttttagt aaattattga
     5041 ttctgtcaat catatattta cccccctctt aagggtcggc cggaaaagga tgcatcagag
     5101 atatctccct ttatcccctg cccgatcatt tataaaatac gccggatatc cgggtgcgtt
     5161 tcacttaacg tattgagtgc aaggggacag ttccttctgt gcctcgatat acagaacaca
     5221 gaaggaccgt ccccctgtgt gtttcagaag gaccgtcccc ctgtgttcgc acagccatac
     5281 atcaaacggt gacaatttgg gaacgtcccc ttttgtcacg gaacgtcccc ttttgtcatg
     5341 cttttattcc ttgccggcag cgacttcaga aagtgccgga ggttcttcct tgcctgatga
     5401 caaagaaaat gttcccttgg caccttggcc catgccggtg tagaaaaagc cctggctctc
     5461 taatgtatcc cttcggtaga tgtttctaaa atcaaagaaa tagggttcct ttagcagttc
     5521 ctttaccctc tttaggttga ggtttctaaa ttggttccat tccgtaatga ttatcagggc
     5581 atcggagcct tccatggcct cgtattcgtt ttggcaatag gttacacgat cctccaggtt
     5641 cttgagcctc cattttgctt ccttgtaggc tatggggtca tagaccctaa agtctgcacc
     5701 ctttttagcc agttcgttta agatggtgat ggagggggct tctctcatat catcggtatt
     5761 gggtttaaag gaaaggccca gtatagaaag ggtccttccc gaaagatccc ctaggacatc
     5821 ctcgaccttt tgggcggcta tcttcttttg tagttcgtta gcttccacgg tcttttctat
     5881 caaggtgaca ggcgcgccat actctttgcc tatctgagcc agagcaaggg tatcctttgg
     5941 gaagcagctg cccccatagc cgggtcccgg gtttaaaaac tttggactga tcctgccgtc
     6001 cctgcccatg gcccttgcca catcctgtac attggccccc accttttcgc aaaggttggc
     6061 cacctcgttt ataaaggtga tcttcatggc caaaaatgca ttggatgaat actttatcat
     6121 ctcggaggtt tcgatattgg tctctacaaa gggggtttcg tttaagtaga gcacgctgta
     6181 tacgttcttc atgatatcca tggccttttt agattctgcc cctatgacca ccctgtcggg
     6241 atgggtaaag tcatggacgg cggaaccctc ccttaaaaat tccgggttgg ataccacatc
     6301 aaaatcatgg gatacccctc tgtctaaaag cccttctgta acccagccct ttaccctttg
     6361 gccggtaccg atgggcaccg tgctcttatt tactattacc ttgtacctgt tcatgtgcct
     6421 tgcaatatct ctggctacgg cttctacgta ttgaagatcg gcgctcccgt catcggccgg
     6481 tggcgtaccc acggcgatga atatgacatc gcactcctct accgcctctt ttatgtccct
     6541 tgtaaacaag agccttttgt agtatacgtt tctctccact atgggcgcaa ggcccggctc
     6601 gtagatgggg attatcccct tttgcagctt gtctatcttt tccctgtcat tatctacaca
     6661 gatgatatta tggccgaaat cagacaggca tgtccccgtc accaggccta cataccctgt
     6721 tcctataact gcgattttgc tcatactaat tcccccataa cccttatcct ctatttatct
     6781 tatttttata atac
//
LOCUS       DASRNX010000030         5896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_483903,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000030 DASRNX010000000
VERSION     DASRNX010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483903"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..617
                     /locus_tag="VFD89_01120"
     CDS             129..617
                     /locus_tag="VFD89_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZJ56826.1"
                     /translation="MSAVILEENKEKFELLQKTINEWKDKEGPLMPVLHSAQEIFGCL
                     SLDVQKFISKELDVPVSDIYGVATFYSQFTLKPKGKYTISVCMGTACYVKGSQAILDE
                     LIKELDLQEGDTSEDGLFTLDATRCLGACGLAPIIMINDDVYGRLEPGEIKGIIDKYR
                     DR"
     gene            620..1171
                     /locus_tag="VFD89_01125"
     CDS             620..1171
                     /locus_tag="VFD89_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ56827.1"
                     /translation="MQDLSLHILDLIQNSVVAGASLIKLTIYEDENKNRLFIDIEDNG
                     KGMDEEAIKRATDPFYTTRSTRKVGLGLPLFKTMAQRCGGDTVIKSVVGKGTRVLSDF
                     QLGHIDRPPFGKLDDTMVTLILCNPEIDFIYSHRVNNEEFKFDTREVRGTIEEIPIEH
                     PEILKWIQSHIKEGLNEINGGVQ"
     gene            1171..1545
                     /locus_tag="VFD89_01130"
     CDS             1171..1545
                     /locus_tag="VFD89_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HZJ56828.1"
                     /translation="MMTLKELEKIRQKTLDSVNLRKDRSGTRVVVGMATCGIAAGARP
                     VLTAFLGEIQNRNLKHITVTQTGCIGVCRLEPMVEVYLPGEDKVTYVNMSPEKVKRVV
                     SEHLVNEQVVQEFTIGAVEKID"
     gene            1567..3363
                     /locus_tag="VFD89_01135"
     CDS             1567..3363
                     /locus_tag="VFD89_01135"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZJ56829.1"
                     /translation="MELYRSHVLVCGGTGCHSSGSADLIEHFDRELRKFGIENEIKVV
                     QTGCFGLCEVGPIVIVYPEGAFYSRVEDEDVTEIVSEHLVKGRIVNHLLYHDSVDEDH
                     EIKSLDEVPFYQKQERVALRNCGVINPENIDEYIAFDGYMALGKVLTEMTPEEVIDLL
                     KESELRGRGGAGFPAGLKWEFTYRAQGDKKFVACNADEGDPGAFMDRSILEGDPHAVI
                     EAMTIAGYTVGADQGYIYVRAEYPIAVQRLTMAISQAREYGLLGKNILGTDFSFDLEL
                     RLGAGAFVCGEETALIASIEGERGEPRPRPPFPAVKGLFGQPTLLNNVETYANIPQII
                     INGPKWLQSIGTEKSKGTKVFALGGKINNTGLVEVPMGTTLREIIYEIGGGIPNGKTF
                     KAAQTGGPSGGCIPVEHIDTPIDYDSLAAIGSMMGSGGLIVMDEDNCMVDIARFFLEF
                     TVEESCGKCPPCRIGTRRMLDLLNKIAEGKGEEEDLDKLEELGEGIKIASLCGLGQSA
                     PNPVLSTIKYFREEYEEHIRDKKCRAGVCQALLQYIILEDKCRSCGLCAKVCPVNAIT
                     GQKKQPYVIDQDPCIKCGACMEKCPFDAIIKR"
     gene            3389..5128
                     /locus_tag="VFD89_01140"
     CDS             3389..5128
                     /locus_tag="VFD89_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HZJ56830.1"
                     /translation="MEMIKVKIDGMEVEVPVGSTALEAARLANIDIPTLCYLKDINEI
                     GACRMCLVEVEGARALQASCVFPVTDGMNIKTNTPKVRTARKLSLELILSNHDRKCLT
                     CVRNQDCELQDLSRRLGVDDIRFEGERYDKPVDSFSPSIERDPNKCILCRRCVAVCRD
                     VQGIGVIGATERGFDTMVEPAFNMSINDVPCINCGQCIIACPVGALREKDETEKVWDA
                     LNNPDLNVVVQTAPAVRVALGEEFGMEMGTRVTGKMVAALRRMGFDRVFDTDFAADLT
                     IMEEGTELLGRLEGGGKLPMITSCSPGWIKYCEHYYPEFLDNLSTCKSPHEMMGAVVK
                     SYYAEKNDWDPAKTFVVSIMPCTAKKFEAKRPELSVSGHPDVDVVLTTRELARMIKEA
                     GMDLTKLPDEDFDPALGDSTGAGVIFGATGGVMEAALRTVHEIVTGEPMENVEVHEVR
                     GIKGVKEAEIKLGDLKVKAAIAHGTANAKALLEQVKSGEKEYHFIEIMGCPGGCVTGG
                     GQPIVKAKTYMEMDPMVVRAKAIYEEDRNLPIRKSHENPSIKKLYEEYLGEPNSKKAH
                     ELLHTHYIKRDVY"
     gene            5460..>5896
                     /locus_tag="VFD89_01145"
     CDS             5460..>5896
                     /locus_tag="VFD89_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ56831.1"
                     /translation="MEKAYGANDIEVLKGLDAVRMRPGMYIGTTGPRGLHHLLWEIVD
                     NGIDEAANGYATRIKVTLAADGSITVEDNGRGIPVDINETAGLPGVEVVFTQLHAGGK
                     FNSENYSYSGGLHGVGAAVVNALSKWLEVEIKRDGKVYRQRFE"
BASE COUNT         1733 a         1118 c         1616 g         1429 t
ORIGIN      
        1 agaatgtttt tgtgttttaa tttgaataaa aaacacggcg gacctaaaca gggatgctcg
       61 catcaaagtg tccccaaggt gcgccgataa taacaattta aaactattat tttggaggag
      121 ggggctaaat gtcagccgta atcttggaag aaaacaaaga gaaatttgaa ttgcttcaaa
      181 agaccatcaa cgaatggaag gataaggaag gacctctaat gccggtgctc cacagcgctc
      241 aggaaatatt tggatgcctg tccctggatg tacaaaagtt tatatccaag gaattggatg
      301 ttcctgtcag tgatatctat ggggttgcaa ccttttattc acagttcacg ctgaaaccca
      361 agggcaaata tacaattagt gtatgcatgg gtacggcttg ctatgtaaag ggttcacagg
      421 ctatattgga cgaattgata aaggaacttg atctgcaaga gggggacacc tccgaggatg
      481 gactattcac cttagatgcc accaggtgtt tgggagcatg cggattagcg cctataatta
      541 tgataaatga cgatgtatac ggaaggctag aacccggaga aattaagggg attattgata
      601 agtataggga tagataacca tgcaggatct gtctttgcat attttagacc ttatacaaaa
      661 ctcagtagtg gcgggtgcca gccttataaa gctgactatt tacgaggatg agaataagaa
      721 tcgactcttc atagatatag aagataacgg caagggaatg gatgaggagg ccataaaaag
      781 agccaccgat ccattttaca ccacccggtc tactagaaag gtgggactgg gattaccgct
      841 atttaaaacg atggcgcaaa gatgcggcgg cgatacggta atcaaatccg ttgtgggtaa
      901 aggtacgagg gtgttgtccg attttcaatt gggacacatc gaccggccgc cctttggaaa
      961 gctggatgat accatggtga ctctgatttt atgcaatccc gagatagatt ttatatacag
     1021 ccatagggta aataatgaag aatttaaatt cgataccagg gaggttcgcg ggactataga
     1081 agaaatcccc atagaacatc ctgaaatatt aaaatggatt caatcccata tcaaggaagg
     1141 actcaatgaa attaatggag gtgtccaata atgatgacct taaaagaatt ggaaaagatc
     1201 agacaaaaga ccttagactc agtcaacctg agaaaggata gatcgggtac cagagtggtg
     1261 gtaggaatgg caacctgcgg aatagccgcc ggtgcaaggc ctgtgcttac ggcatttctg
     1321 ggggagattc aaaacagaaa cctcaaacat ataactgtca cccaaaccgg atgcattggg
     1381 gtatgccgtt tagagcccat ggtagaagta tatctgcccg gtgaggacaa ggtaacgtat
     1441 gtcaatatgt cgcctgagaa ggtaaaaagg gtggtcagcg agcatctggt aaatgaacaa
     1501 gtagtgcaag aatttaccat aggggcagta gaaaagatag attaataggt gaggaggctt
     1561 aaagatatgg aactttatcg ttctcatgtt ttagtctgtg gggggaccgg atgtcattct
     1621 tcgggctcag cagatctgat agaacatttt gatagggaat taagaaagtt tggaatagaa
     1681 aatgaaataa aggtagtaca gaccggttgt tttgggctat gtgaagtagg acctattgta
     1741 atagtatacc ccgagggagc attctacagt agggtcgagg atgaggatgt aactgaaatc
     1801 gtttcggaac acctggtcaa aggtaggata gttaaccatc tcctatacca tgatagtgtg
     1861 gatgaggacc acgaaatcaa atccttggat gaggtgcctt tctaccaaaa gcaggaaagg
     1921 gtagctctga ggaattgcgg tgttataaat ccggaaaaca tcgatgaata tatagcattt
     1981 gacgggtata tggctttggg gaaggtatta acggagatga cccccgaaga ggtcatcgac
     2041 ctcttaaagg aatcggagct taggggacgg ggcggagcag gatttcccgc cggtctaaaa
     2101 tgggaattta cctatagggc ccaaggggat aaaaaatttg tagcctgtaa cgcggacgag
     2161 ggagaccccg gagcatttat ggaccgaagc attttagagg gagaccccca cgcagtgatc
     2221 gaggctatga ccattgcagg atatactgta ggtgcagatc aaggatacat ctacgtaagg
     2281 gcggagtatc ccattgcagt acagaggctc accatggcta taagccaagc ccgggaatat
     2341 ggtctgttgg gcaagaacat cttggggacg gactttagtt tcgatctaga attgagactt
     2401 ggggcaggcg cttttgtgtg cggggaagag actgcactaa ttgcctccat cgagggtgag
     2461 cgcggcgaac caagacctag acccccgttt cctgcggtaa agggtctctt tggtcaaccc
     2521 acccttctta acaatgttga aacttatgcc aatatcccac aaattattat caatggccct
     2581 aagtggctcc aaagcatcgg cactgaaaag agtaagggta ccaaggtctt tgccttgggc
     2641 ggaaagataa ataatacagg acttgtggag gtccccatgg ggacgacttt aagggagatc
     2701 atctacgaga ttgggggggg tatccccaat ggcaaaacct tcaaagccgc ccaaaccggc
     2761 ggaccttccg ggggatgcat ccctgttgag catatagata cccccatcga ttatgattcc
     2821 ttggcagcca ttggttccat gatgggatcg ggtgggctga ttgttatgga cgaagacaat
     2881 tgtatggtgg atattgcgcg attcttcctg gagtttaccg ttgaagaatc ctgtggaaaa
     2941 tgccctccat gtcgtatagg aaccaggaga atgctagacc ttttaaataa gattgccgag
     3001 ggtaaaggcg aagaagagga tttggataag ctggaggagc tgggcgaggg gattaaaata
     3061 gcttcattat gcggccttgg acaatcggcg cccaacccgg tactgagcac tataaaatat
     3121 ttccgtgagg aatatgagga gcatataagg gataaaaaat gcagagccgg ggtctgccag
     3181 gcactcctcc aatatattat tcttgaggat aagtgcagaa gctgtggtct atgtgccaag
     3241 gtttgtcccg ttaacgccat taccggacag aagaaacagc cttacgttat agatcaggat
     3301 ccctgtatca aatgcggggc ttgtatggaa aaatgtcctt ttgatgccat tattaaaagg
     3361 taacccggaa cttaaaagga gtgaaaacat ggaaatgatt aaggttaaaa tcgatggcat
     3421 ggaagtggaa gtacctgtcg gttctacggc tctggaagct gccaggttgg ctaatataga
     3481 tatacctacc ctatgctatc ttaaggatat aaatgagatc ggtgcatgtc gcatgtgttt
     3541 ggtagaagta gagggggcca gggcccttca ggcatcctgt gtatttccgg ttaccgacgg
     3601 aatgaatatc aaaaccaata ctcccaaggt aagaacggcc agaaaactga gccttgagct
     3661 tattctatca aaccatgaca gaaagtgttt gacctgtgta aggaatcagg attgtgaatt
     3721 gcaggatcta tcaagaaggc ttggggttga tgatatacgc tttgagggcg aaaggtacga
     3781 taaacccgta gactcattct cgccctctat tgaaagagac ccaaacaaat gcatcctgtg
     3841 caggcgttgt gtagccgtat gcagagatgt tcagggtata ggcgtaatcg gggcaactga
     3901 gagaggcttt gataccatgg ttgagccggc ctttaatatg agcataaatg atgttccctg
     3961 cataaactgc gggcagtgca tcatagcatg ccctgtaggt gccctgcggg agaaggacga
     4021 aactgaaaag gtatgggatg cccttaacaa tccggatctt aacgtggttg tccagaccgc
     4081 tcctgctgtt cgtgttgccc ttggcgaaga atttggtatg gaaatgggaa ccagagtaac
     4141 gggtaagatg gtagcggccc ttaggcggat gggatttgat cgtgtgtttg ataccgactt
     4201 tgctgcggat ctaaccatca tggaggaggg tacagagctc ctaggaaggc ttgaaggtgg
     4261 cggtaagctg cccatgatta cttcttgcag tcccggttgg ataaaatatt gtgagcatta
     4321 ttaccccgag tttttggaca atttatctac atgcaaatcc ccccatgaga tgatgggggc
     4381 tgtagtaaaa tcctattatg ctgagaaaaa cgattgggat ccggcaaaaa cctttgtagt
     4441 gtccattatg ccttgcactg cgaagaaatt tgaagccaag cgtcctgagc tgagcgtctc
     4501 gggtcatccc gatgtcgatg ttgttctaac taccagagag ctggcaagga tgattaaaga
     4561 ggcgggtatg gatcttacaa agctgcctga cgaggacttt gatcccgccc ttggggattc
     4621 aacaggagcc ggtgttatct tcggtgcaac cggtggcgtt atggaggctg ccctgcgtac
     4681 cgtgcatgag attgtcacgg gggagcccat ggaaaatgtt gaggtacacg aagttcgcgg
     4741 aatcaagggg gtaaaagagg ctgaaattaa gctgggagac ctaaaggtaa aggccgccat
     4801 agcccatggc actgccaatg ccaaggccct gttggagcag gttaaatccg gtgagaagga
     4861 atatcatttc atcgagatca tgggatgccc aggcggatgc gtaacagggg gaggtcagcc
     4921 catagtaaag gctaagacct atatggaaat ggatcctatg gtggtaaggg ccaaggccat
     4981 ctacgaggag gaccgaaatc tgcccattag aaaatcccat gagaatcctt caattaaaaa
     5041 actgtacgaa gagtatctgg gcgagcccaa cagtaagaag gcccatgaac tgcttcacac
     5101 ccactatatc aagagggatg tctactaact gaggggtcat gctttcataa ttatgtacga
     5161 attatgtacg atgaactatt caaatgcggc gctctatggg taccgggcct aataatggat
     5221 ttgggtctag gctatggtac cgtccggagc tatatagtct actagtggat gttttcgatt
     5281 taatcgtaca atagaggtat aaagaaaaag agaatcctaa cccgaggatt ctttttttct
     5341 gtccataggc atgaaaggct tgatcctttg catctttggt tgactttaac aggtgataaa
     5401 cttataatga aaaggaagag aaagaggaac atacgttcat taggcttgga ggtttactga
     5461 tggaaaaagc ttatggtgca aatgatattg aagtattgaa aggtttggat gccgttagga
     5521 tgcgtcccgg gatgtatata ggtacaaccg ggcccagggg gcttcatcat cttctatggg
     5581 agatagtaga caatggaata gacgaggctg ccaacggata tgcaacgcgg attaaagtga
     5641 ccctggctgc ggatgggagc ataacggtgg aggataacgg ccgaggaata cccgtagata
     5701 taaacgaaac cgccgggctc cccggcgtcg aagtggtttt tacccagctc catgcggggg
     5761 gaaagtttaa cagtgaaaat tacagctact ccggcggtct ccatggcgtt ggagccgcag
     5821 tggtcaatgc attgtctaag tggctggagg tagagataaa aagggatggt aaggtctaca
     5881 gacaacgctt tgagag
//
LOCUS       DASRNX010000031        14348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_695504,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000031 DASRNX010000000
VERSION     DASRNX010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14348
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695504"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3085)
                     /gene="addB"
                     /locus_tag="VFD89_01150"
     CDS             complement(<1..3085)
                     /gene="addB"
                     /locus_tag="VFD89_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HZJ56832.1"
                     /translation="MSLRLIYGRSGSGKSHFCMQEIRTILEDQLRGQTKGPLILVVPE
                     QYSMQAERDLVQAAPKGGAIDAQVLSFRRMAYRVFGEVGGFTRRHISPSGKSMLIYRI
                     IEELKDSLNYFDKVAGQSGFVSTISELISELKRYNITPNALEVLHTQIEDEALGEKIK
                     EIGAIYKRFEETVHLRYIDADDDLTLMAEKLPESTLLDGAEIWIDEFSGFTPQEYSVI
                     GEILKKAKRVSVCLCTDYLFDEMPFSSGDVFAPVRNTALRLRELARENQIDIEMPVTF
                     KEEPFHRYKDSQELAHLEGYFFTFPYKRYTMPTKDVEIFTAGNIYTEIEEAARNIQSL
                     CRDGGIRYKDIAVVTRDLTEYEGLISAIFPQYSIPFFIDRKKPIDSHPLVTLVISMMD
                     IFVHNWSYESVFRYLKTGLTGIVREDVDVLENYILANGIRGSQWTRGEDWDYRPDSGF
                     YSGKITGREHSVIDRVNNTRQNILKPLMDFRRNTRGRTPVRDMCSAVYEFLCDLGVPE
                     LIEERIDDLREAGSLNLANEYAQIWNIVMEVLDQTVEAMGEESMGIERFMEVLSIGFS
                     EHKIGLIPPSLDQVLVGSVERSRSHEIKALFILGVNDGVFPAPPGEEGILSDRDRQTL
                     RGMGVELAQDTRTRAFEEQFIIYSTLSTTGGRLFISYPIADHEGRTMRPSIIISRLKK
                     LFPKIRERNNLSETSSQEDTFNMVSAPLPTFNQLISAVRRGTLGHGVDPLWKHVRSWY
                     EGQESWGERVERVVEWMGYSNQAEKVGTKRAAMLYGSPMYSSISRLEQYAACPFSYYL
                     RYGLGAKERRVYALAPPDMGSFMHDCIDVFSSRIKDAGIGWEELDRDWCHGEISSIVD
                     ELLEKKMGWLVNSSDRYRYLIVRLKRIVARAIWIIREHIIRSGFEPIGYEVSFGESED
                     DIFPPIVLELPSGDKIHLVGRIDRVDAHKGKDGTYIRIIDYKSGSRSFRLSDVYYGLQ
                     IQLVTYLGAVLDSGKGILGDKLLPGGMLYFKIDDPLIRLTGEADEEDIERAIMKKL"
     gene            complement(3393..4355)
                     /gene="eda"
                     /locus_tag="VFD89_01155"
     CDS             complement(3393..4355)
                     /gene="eda"
                     /locus_tag="VFD89_01155"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HZJ56833.1"
                     /translation="MDVLGRIGKIGLVPVVVMEKASDAPPVAAALRDGGIPIMEITMR
                     TDAGIDAIKAVKASNPEVLVGAGTVLTLDKCKESVDAGAEFIVSPGFNPKIVDWCLEN
                     DVPITPGCVTPTEIEMALDKGLKVLKYFPAGVYGGIKGCTALQGPYKSMGLKFIPTGG
                     ISLKNLDEYSDKEFIHAIGGGWLSNSKDIAAGNFDAIRETARISIAQLLGFEFAHVGI
                     NTVNDADSKNIAEEFDTVFGFGVKYGNSSNFAGSGIEVMKGAGLGKSGHLAVKTNNVG
                     RAVHYLEGQGFTFDMESAKYKGDAMIAVYLKEEIGGFAVHLLQK"
     gene            complement(4742..4921)
                     /locus_tag="VFD89_01160"
     CDS             complement(4742..4921)
                     /locus_tag="VFD89_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56834.1"
                     /translation="MDVNFVIESPVDKVKSRSHKMIEKSLEDGGYALGTGNSVPEYIP
                     AEKYLAMISAIHDVY"
     gene            complement(4989..5318)
                     /locus_tag="VFD89_01165"
     CDS             complement(4989..5318)
                     /locus_tag="VFD89_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56835.1"
                     /translation="MDYEVIKPEFSRDVADGDEVLKIVSAGEYSYGKWHCDIDNIEDN
                     ATYRFSIKYFSEHVPVEGASINAVFTWIKEDGEWLERGYIDGLEALDDGWKILSKTIS
                     APREAFL"
     gene            complement(5417..6643)
                     /locus_tag="VFD89_01170"
     CDS             complement(5417..6643)
                     /locus_tag="VFD89_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZJ56836.1"
                     /translation="MSQLTSRQRVRLSLNHQEPDRLPIDFGAMRSTGINAIAYNKLTD
                     YLGMQGSQTRVYDIFQQLAEPEEAIMKIMGADVIQLHRLWPSFGIRIDSWKPWKLMDG
                     SDCLVPKDFNPVENAQGDLDIVEGARVVARMPKGGYYFDLMHHPYENVKSMEDINAIP
                     LSEITEEELTYLRDRARELHDKTDYAILGCFGGNILEAGQSDWGYERFFIELALNPDL
                     VHYYLERKTQAYLRDLEKYLNAVGDYIDVIQFGDDLGTQENTQISLQMYSDMIKPYHT
                     RQYQYVRNNFPHVKVFLHSCGAIFDLIPDLIEAGVEVLNPVQLSARNMDPIKLKREFG
                     KHLTFWGGGSNAQTTLTFGSLKDIRDEVEELIGIFSPGGGFVFTQVHNIQANISPEKI
                     MEVYNTANRLGRQIYR"
     gene            complement(6810..8321)
                     /locus_tag="VFD89_01175"
     CDS             complement(6810..8321)
                     /locus_tag="VFD89_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HZJ56837.1"
                     /translation="MPINRTLRIHETDNVVVALTPIKRGDILDIDNKKIEAIEDIGVG
                     HKMSVHDIMIDEDIIKYGVSIGHATMPIKVGSLVHTHNIVTNLSGILDYTYESQFQGL
                     EKPIDDNIHTFSGYLRPDGDVGIRNEIWIINTVGCVNKVAERLVRSAREDFRGRGVDG
                     IHFFAHPYGCSQLGDDHKNTQNILAGLANHPNAGGVLVLGLGCENNNIDEFKKVLKER
                     GPIDTNRIRFLNTQDVEDEHEVGMEILGELVTYAEGFVREEVTISRLRVGLKCGGSDG
                     FSGITGNPLLGAFSDDLVSHGGTTILTEVPEMFGAETILMNRCISEEVYQKCVSMIND
                     FKEYFVRHGQVIYENPSPGNKEGGITTLEEKSLGCTQKGGTGQVVDVVGYGEPVTLPG
                     LNLLDGPGNDIVAVTALAAGGAHMVLFTTGRGTPLGGPVPTVKVSTNSELAMKKKHWI
                     DFDAGVLLDNRSMESVSEEFFEYIIDVASGKTLTRNEERDFREIAIFKDGVTL"
     gene            complement(8668..10188)
                     /locus_tag="VFD89_01180"
     CDS             complement(8668..10188)
                     /locus_tag="VFD89_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HZJ56838.1"
                     /translation="MKRLNRELIDKGSGLFKDVGVGNLEAMPERIVQFGEGNFLRAFV
                     DSMVDGMNRQGLFMGKVVVVQPIPHGLVDVLNEQDGLYTLLLRGIDKGRITEEKRIIT
                     SVSRGINPYEDWKGLLSCAAQPEMRFMVSNTTEAGIAYVEEGMPTDSCPTSFPAKVTA
                     FLYERFKAFSGCPDRGMIILPCELIDRNGDSLKEIVLRYADEWDLGGEFTRWIKAHNH
                     FLNTLVDRIVTGYPKDEIDELNRELGYEDRLLDTGEPFHLWVIEGDERLSKELPLTEA
                     GFNVVWTEDLTPYRTRKVRILNGAHTMTALMAYLYGLDTVRQCIEDDTVLSYMKKGLY
                     DEILPILDFPKDEKQEFADAVLERFNNPFIEHNLLSISLNSVSKWKVRVLPSLIESVD
                     LRDSLPKVLTFSLASLLAFYRGTEIMGGALIGERMGEQYTIQDDGDVLEFFRSLWDEY
                     ESSGNLGLLCTRALGKKGFWDMDLNRLPGMTEKVREYLGQILNTGMANAVRELIGK"
     gene            complement(10202..11488)
                     /locus_tag="VFD89_01185"
     CDS             complement(10202..11488)
                     /locus_tag="VFD89_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HZJ56839.1"
                     /translation="MNGMIRLSITSDDGQGLHEERIRKALTKSLKGHDRKLERVLLIP
                     PDLTRAHSGAGDITGMYYDILKDSCQVDIMPALGTHMAMTHEEIDLFFGDDIPKDSFK
                     VHNWRTDVVKVGQVPGQYISEISGGLVDEPIDVEINKILLDESYDLIISIGQVVPHEV
                     VGMANYSKNIFVGCGGMDIINKSHMLGAVFGMERIMGRDHSPVRKVFDYCEKHFTKGM
                     PLMYVLTVTTTREDNVDIHGLYIGNERSLFEEAVKLSQEKNLEYVDRPLKKVVVYLDE
                     TEFKSTWLGNKSVYRTRMAIEDGGELIVLAPGVRQFGEDQENDRLIRKYGYVGRLKVL
                     ELFNENQDLKENQSVAAHLIHGSSDDRFSITYAPGHLTREEVEGINYNYMSLKEALEK
                     YDPKKLRDGYNTLEDGEEVFYISNPALGLWADRSKF"
     gene            complement(11758..13032)
                     /locus_tag="VFD89_01190"
     CDS             complement(11758..13032)
                     /locus_tag="VFD89_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZJ56840.1"
                     /translation="MVGARLRGQLRETGNILKGFKGNARACIMMEPLWGIPYNLFITY
                     ASVYMIALGSTEKQIGLIASVGLIFQMFFSLVGGHITDKLGRKRTSLVFAIISWTIPT
                     LIWAMAQNFYYFLIAAIINSAVRVVHTSWTCLFVEDTPPEGRVHVFTWTQIAGILSGF
                     VAPVAGILVSRYQLVPAVRGLYFFAFFSMTSMFIIRNYLVHETGQGLIRMKESKNVGL
                     FDSLEDYKRAATQLISTPYTMIVFLLSIFSNIHTVIRTNFFAILLTKKLQFSQGSIAL
                     FPMIQSGIMLLVLLFVMPALGRISLKKPLLGSFASLIISNLLLVVSPHKNYFVLIIST
                     VFMAFGTAVSFPLIESMLANAIDDKERAKTMSILYVILFAVTAPFGYIGGVLASVSEE
                     LPFVMMGIIFVICIFLIFILDRLEKPGDVHDN"
     gene            13274..14341
                     /locus_tag="VFD89_01195"
     CDS             13274..14341
                     /locus_tag="VFD89_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ56841.1"
                     /translation="MKKLNLAIIGQGRSGRDIHGKYFLTDSDRFKVVAVAEALEDRRN
                     RAAEEYGCDTYENYQELFKRSDIDLVVNASFSHMHAHTTIDLLNHGFNVLTEKPCAKT
                     PEEVQAMMDAAEANKRMFAVFQQSRFAPYFEKVKSVMDSGILGRIVQISISFNGFARR
                     WDWQCCQEFVAGSLYNTGPHPMDQALNLLDYYDGTPRVFCKMDRANTFGDAEDYVKVI
                     LTAPDRPLIDVEISSCDAYPTYTYKVQGTRGGLEGNMTHIKWRYYDERVAPKRELIKT
                     PLMDDQGLPMYCGEKLYWAGETWSVEDPGVFDHAVKRFYDNIYDHLLNGTPLVVTPQQ
                     VKQQIHVINECHEQNPLSKFC"
BASE COUNT         3693 a         3785 c         2884 g         3986 t
ORIGIN      
        1 tcagcttttt catgatggcc ctttctatat cctcctcgtc tgcctcaccg gtcaaccgaa
       61 tcaggggatc gtctatctta aaatacagca tcccgccggg caacagctta tcccccaata
      121 ttcccttgcc gctgtccaaa acggccccca agtaggtgac caactgtatt tgaagaccgt
      181 agtagacatc ggataatcta aaggatctgc tgccggattt atagtctatg atccgtatgt
      241 aggttccatc tttcccctta tgggcgtcga ccctgtctat cctgcccacc agatggatct
      301 tatctccaga gggcagctcc aatacgatgg gtggaaatat atcatcctcg gattcaccga
      361 aggacacctc gtatcctatg ggctcaaatc cgctccttat tatgtgctcc cttataatcc
      421 aaatggccct tgctacaatc cgctttaggc ggacgatcag atatctgtag cgatcgctgc
      481 tgtttaccag ccagcccatc tttttttcaa gcagttcatc cacgatggag gagatttccc
      541 catgacacca atccctatca agctcctccc agccaatgcc ggcatccttt atcctggaag
      601 aaaacacatc tatacagtcg tgcataaagc tccccatatc cgggggcgca agtgcataaa
      661 cccttctttc cttggcaccg aggccatacc ttaggtaata ggaaaacggg catgccgcat
      721 actgctccaa ccttgatatg ctggaataca tgggggagcc atagagcatt gcagcccttt
      781 ttgttccaac cttctccgct tggtttgaat agcccatcca ctctactacc ctctctaccc
      841 tctcccccca ggattcctgt ccctcgtacc agcttcttac atgcttccat aagggatcaa
      901 ccccgtgccc cagcgttccc cgtcttacgg cggatatcag ctgattgaag gtgggcaggg
      961 gcgcggagac catattgaat gtatcctcct gactgcttgt ctccgataaa ttattgcgtt
     1021 cccttatctt agggaaaagc ttctttaacc ttgagataat tatggaaggt ctcatggtcc
     1081 tgccttcatg gtcagcaatg ggatagctta taaagagtct tcccccggta gtggaaaggg
     1141 tcgaatagat tataaattgc tcttcaaagg cccgggtcct ggtatcttgg gccaactcca
     1201 cacccatccc tcgaagggtc tgtctgtccc tgtccgataa tattccttcc tcccccgggg
     1261 gcgccggaaa gaccccatcg ttgaccccta agataaacaa ggcctttatt tcgtggctcc
     1321 tggacctctc cacgcttcct accagcacct gatcgaggga gggcggaatc agtcctatct
     1381 tatgttcgct aaagcctatg gacaggacct ccatgaacct ttcgattccc atgctctcct
     1441 cccccatggc ttctaccgtc tgatccagca cttccatgac gatattccag atctgagcat
     1501 actcattggc caggtttaag gagcctgcct cccttaaatc atcgatccgc tcctctataa
     1561 gttccggtac ccctaaatcg cataaaaatt cgtagacggc actgcacatg tcccgcaccg
     1621 gggtacgacc cctggtgttc cttcgaaaat ccataagggg cttcaatata ttttggcggg
     1681 tattgttaac cctatcaatt acggaatgct cccgccccgt tattttcccg gaataaaacc
     1741 ccgaatccgg cctatagtcc cagtcctcgc cccttgtcca ttggctcccc cttatgccgt
     1801 ttgccaggat ataattttct aatacgtcaa catcctctct gactattccc gtcaggcccg
     1861 tctttagata cctgaacacc gactcatagg accaattatg tacaaagata tccatcatgg
     1921 atatcaccaa ggtaactaag gggtgactgt ctatgggctt tttcctgtct atgaaaaagg
     1981 gaatggaata ttgggggaat atggccgata taaggccctc atattccgtt aagtccctgg
     2041 tgaccaccgc aatatcctta taccttatcc ccccatctct gcacagggat tggatatttc
     2101 gggccgcctc ttctatttcc gtatagatgt ttccggcagt aaatatttct acgtccttcg
     2161 tgggcatcgt gtatctttta taggggaagg tgaaaaagta gccctccaag tgtgccagct
     2221 cttggctgtc tttatacctg tggaaaggct cctctttaaa ggtcaccggc atctctatat
     2281 ctatttgatt ctccctagcc aattccctca gcctcagggc cgtatttctc accggtgcaa
     2341 agacgtcacc gcttgaaaaa ggcatctcat cgaatagata atcggtgcag aggcacacgc
     2401 tgacccgttt ggccttcttt agtatttccc ctatgacgct gtattcttgg ggggtaaagc
     2461 ccgaaaactc atcgatccaa atctctgccc catctagcag ggtggattcc gggagctttt
     2521 cagccatcaa ggtaagatca tcatctgcgt ctatgtatct taaatgtacc gtctcttcaa
     2581 accttttata tatggctccg atctccttta tcttttcccc tagtgcctcg tcttcgattt
     2641 gtgtatgcag cacttccagg gcattggggg tgatattata tctttttagc tcggagatga
     2701 gctccgatat ggtgcttacg aacccagatt gccctgctac cttatcgaag taattaaggc
     2761 tgtcctttag ctcttcaatg attctgtata tgagcatgga tttgcccgat gggcttatat
     2821 gccttctggt aaatccgcct acctcaccaa agaccctgta tgccatccgc ctaaaactga
     2881 ggacctgggc atctatggct cccccctttg gtgcagcctg aaccaggtcc ctttctgcct
     2941 gcatggaata ctgctcaggc acaaccagga tcagaggtcc cttggtctgt ccccttaatt
     3001 gatcctccaa tatagttcgg atttcctgca tacaaaagtg gctcttacca ctgccggatc
     3061 tgccgtatat caacctcaaa ctcatgaaac aaaaccccct gctcttcgtg tttcccctac
     3121 tgccaaaatg gttgtcccta taggtatcat cgtctggcct gtaacctaat tttatcacaa
     3181 gctgtcttga aattacaatt atttaaatga aaagggggct gtcagccccc ttacaaaatt
     3241 ttatgtcatt aatctgatgt tatgtgcagt ccggctaata cctcttatag ggagtcaccc
     3301 tgcaaaataa tcccaacaga gtcggcctac taggcctcct acattccaac gcctttagcg
     3361 gattggtgca atcgtccctt tacggtcgat tcctattttt gaagaaggtg aaccgcaaat
     3421 cctccgatct cttccttgag atagacggcg atcatagcgt cgcccttata ctttgcgctt
     3481 tccatgtcaa aggtaaagcc ctggccttct agataatgga cggcgcgccc aacattgttg
     3541 gtctttactg ccagatgtcc gctcttgcca agacctgcgc ccttcataac ttcaatgccg
     3601 cttccggcaa agtttgaact gttgccatat ttgacaccaa agccgaacac cgtatcaaac
     3661 tcctcggcaa tatttttaga atcggcatca ttaactgtgt tgattcccac atgggcaaac
     3721 tcgaatccca agagctgtgc gatggatatc cttgccgtct cacgaatcgc atcaaagtta
     3781 cccgcagcga tatctttaga attgctcagc caacctccgc caatggcgtg tatgaattcc
     3841 ttatcggaat attcatcaag attttttaag ctaattccac cggtagggat aaacttaagg
     3901 cccatggact tgtaggggcc ctgcaaagca gtgcagccct ttatgccacc gtagacgcct
     3961 gccgggaaat acttaagaac cttaaggccc ttatccagcg ccatttcgat ttccgtcggg
     4021 gtaacgcatc cgggggttat gggcacatca ttttctaaac accaatcgac gattttggga
     4081 ttaaaccccg ggctcacgat gaactctgca ccggcgtcaa cggattcctt gcacttatcc
     4141 agcgtaagaa ccgttcctgc ccccactaag acctcgggat ttgatgcctt caccgctttg
     4201 atggcatcga ttccggcgtc tgtgcgcatg gtgatctcca taatgggaat cccaccgtca
     4261 cggagggctg cagcaaccgg cggggcatcc gaggcctttt ccataacgac caccggaact
     4321 aaaccgattt tacctattcg tcctaaaaca tccatgataa atactcctct caataaaaag
     4381 taatttgtat ccgcccttta taacaataag cagcggataa ggactttttg taattagaat
     4441 ggaaaaccac ctttaacttc caccctaata ctatagtaag ggatttcctg cgtaaaatca
     4501 ataacctttg acgaaatcat atgaaaggcc ccccatgccc ttctataaat aaacaatagg
     4561 ttgactatgg ataaacaata cagaaactaa atttactgat atgcagattg caatgactct
     4621 gcacaatttc atagcgatga attttatagt tggcgtcgtt tatcttttaa cttatcggca
     4681 cgaaaacttt aatggatcaa tgttctctta cggtaaactt tatagttggg gttgtgtatc
     4741 actaatacac atcatgaata gctgaaatca tagctaaata cttctctgca ggtatgtatt
     4801 cgggtacact atttcctgtt cccaaagcat agccgccatc ttccaggctc ttttctatca
     4861 tcttatggga tctgctcttt actttatcca ctggggactc tattacaaaa tttacatcca
     4921 tacccccaag gattgcaatc ctgcccttcc atctgtcata ggaatcctct actcttagga
     4981 tgttgtcctc aaaggaatgc ttcccgaggt gctgatatag tcttgcttag aattttccat
     5041 ccgtcatcta gggcttccaa gccgtctata tacccccgtt ccagccattc tccgtcctct
     5101 ttaatccatg taaatacggc gtttatactc gctccttcaa ccggtacatg ttcagagaaa
     5161 tattttatgc tgaatctata ggtggcatta tcttcgatat tatctatatc gcaatgccac
     5221 ttaccatagg agtattcccc cgccgaaact atctttaata cctcatcccc atctgccaca
     5281 tccctggaaa actccggttt gatgacttca taatccacgc tttgggctcc aatatacgtc
     5341 gatcccccta tcgaccccta ttaatttaaa tagctatttg ggtttgattg gctgatccca
     5401 tataagggaa tgtcccttat ctatatatct ggcgccccaa ccgatttgcg gtgttataaa
     5461 cctccatgat tttttcggga gatatatttg cctggatatt atgcacttgg gtaaacacaa
     5521 acccgccccc gggagaaaat atccctataa gttcttctac ctcatctctg atatccttaa
     5581 ggctaccgaa agtcagggtg gtttgggcat tgcttccccc accccaaaat gtcaagtgtt
     5641 taccaaattc ccttttaagt ttaatggggt ccatattgcg tgccgatagc tgaacaggat
     5701 ttaagacttc gacacctgcc tctattagat cggggatcaa gtcaaagatg gccccgcagg
     5761 aatgcaaaaa tacctttacg tgaggaaaat tgtttcttac atattgatac tgtctggtgt
     5821 gatatggttt gatcatatca ctatacatct gtagggatat ctgggtgttc tcttgagtcc
     5881 caaggtcgtc cccgaattgt atgacatcga tataatctcc gacggcattt agatactttt
     5941 ccagatctct taaataggcc tgggttttcc gctcaaggta gtagtgaact agatcggggt
     6001 ttagagccaa ttctataaaa aagcgttcat atccccaatc cgattgaccc gcctctaaga
     6061 tattaccgcc aaagcacccc agaatggcat aatccgtttt atcgtgcagc tccctggccc
     6121 tatcccttag gtaagtaagc tcttcttcgg taatttcgga taggggtatg gcattgatat
     6181 cctccatact tttcacattt tcataggggt ggtgcatgag atcaaagtag taccctcctt
     6241 tgggcatcct ggcaacaacc ctggctcctt caacaatatc caaatcgcct tgggcgttct
     6301 ctaccgggtt aaagtccttg gggaccaaac aatcggatcc gtccatcagt ttccacggct
     6361 tccaactgtc gattcttatc ccaaaagacg gccagagtct gtgcagctgg atgacatctg
     6421 cccccataat cttcattatg gcctcttccg gttctgccag ctgctggaaa atatcgtaga
     6481 cccttgtttg gcttccttgc atcccaaggt agtccgtcag tttgttatag gcgatagcat
     6541 ttatgcccgt ggatctcata gccccaaaat caatgggcaa tctatcaggt tcctggtggt
     6601 taagggacag tcttaccctt tgccttgacg ttaattggct cataattaaa cccccttttt
     6661 atccgccctg cggatgaata attgcatggg aaccttgcac cttatgctgg tttagcatat
     6721 aacggcaatg actactgaac gcttattaat agttcattat ctttattgcg ctgtaaatgg
     6781 tgaaccagtg caatagggac cccctaagct catagcgtta ccccatcctt aaatatagca
     6841 atctcacgaa aatcccgctc ctcatttctg gtcaatgtct tcccggatgc cacatctata
     6901 atgtattcaa agaattcttc cgatacgctc tccatactcc ggttatctaa aagaacaccg
     6961 gcatcaaagt caatccaatg cttcttcttc atagccagtt cgctattggt agaaacctta
     7021 acggtgggta ccggaccacc taggggtgtt ccccttcccg tggtaaatag caccatatga
     7081 gcaccaccgg cagccaatgc cgttaccgcc actatatcgt ttccaggtcc gtccaagagg
     7141 tttagacctg gcagagttac cggttctccg tagcctacca catcaactac ttgcccggtt
     7201 ccgccctttt gagtgcaccc taaggacttc tcttccaagg tggtgattcc cccctccttg
     7261 ttccccgggg aaggattctc gtatataacc tggccgtggc gaacaaagta ttctttaaaa
     7321 tcatttatca tggatacgca tttttggtat acttcttcag atatgcaacg gttcatcaat
     7381 atggtctctg cgccgaacat ctcggggacc tctgtcaata tggtagttcc gccgtgggac
     7441 accaaatcgt cggagaaggc acccaataag gggttgccgg taatcccgga aaatccgtca
     7501 gagcccccgc actttagccc taccctaagc cttgatatgg tgacttcttc cctgacaaag
     7561 ccctcggcgt aggtcacaag ttcccccaaa atctccatcc ccacctcgtg ttcgtcctca
     7621 acgtcctggg tatttaaaaa ccttatccta ttcgtgtcta tggggcctcg ctcttttaat
     7681 acctttttaa actcatcgat attattgttt tcgcatccca atcccagtac tagaacaccg
     7741 ccggcgttgg ggtgatttgc cagacccgcc aaaatgtttt gggtgttttt atgatcgtcc
     7801 cccaactggg agcaaccata ggggtgggca aaaaagtgta tgccgtctac cccacgtcct
     7861 ctaaaatcct ccctggcaga acggaccaac ctttcggcca ccttgtttac acatcctacg
     7921 gtgtttataa tccatatttc attccttatc cctacatccc cgtcgggcct caaataaccg
     7981 ctgaaggtat ggatgttatc atctatgggt ttttcaaggc cttgaaattg ggattcatag
     8041 gtataatcta agattccgga taaatttgtg actatattat gggtatgcac caggctgccc
     8101 acctttatag gcatggtagc atgcccgatg gacaccccat atttgatgat atcctcgtct
     8161 atcattatat catgaacgga catcttatgt cctaccccaa tgtcctctat cgcttcgatt
     8221 tttttattat ctatgtccag tatatcgccc ctttttatgg gcgtcagggc aactacaaca
     8281 ttatcggtct cgtgaatcct taaagtacga ttaattggca ttgtaccctt tccactcccc
     8341 tataatttac tagattaact ctttacagcg atcgattatc tatggataat agactattaa
     8401 aaggcggatg actccattga tctggtttct ggaaattaga aattggaaat tagaaattga
     8461 gggttagaaa ttaaaagccc ggttccacgg agctattgct gagggatgga tttgtggggg
     8521 tagattcccg atgtagtttg gaagacgccg gaataaatca tcttcccatg caaaataagc
     8581 agggtgcctt aagccgattc caccggcagg aggtcggatt ggaagcgata ggcttttcaa
     8641 atggaatgag ccttggcttt tctcaaatca ttttcctata agctccctaa cggcattggc
     8701 catccccgta ttcaatatct gccccagata ttccctaacc ttctcagtca ttccgggtag
     8761 cctattaagg tccatatccc aaaaaccctt tttccctaag gccctggtgc ataacaagcc
     8821 caggttaccg ctgctttcat attcgtccca aaggctccga aaaaactcca acacgtcccc
     8881 atcgtcttgg atggtgtatt gctcccccat cctctctcca ataagggcac cgcccataat
     8941 ctcggttccc ctataaaaag ccaataaaga ggccaaggag aaggtcaaca ccttcggcag
     9001 actgtccctt aaatcgacgc tttctatgag cgaaggcagg accctaacct tccatttgga
     9061 gacggaattt agggagatac taaggaggtt gtgctctata aaggggttat taaatcgttc
     9121 cagtacggcg tctgcaaatt cttgcttttc atccttaggg aaatctaaga taggcaaaat
     9181 ctcatcatat aatccttttt tcatatagga cagtaccgta tcatcttcta tacactgcct
     9241 tacggtgtcc agaccataca ggtatgccat aagtgcggtc atggtgtggg ccccgtttaa
     9301 aatccggacc ttcctcgttc tgtacggcgt taaatcctcc gtccaaacta cattgaaacc
     9361 ggcctcggtt aagggtaatt ccttggaaag tctctcatcc ccctcgatga cccagagatg
     9421 aaagggttcg cctgtgtcaa ggagcctgtc ctcgtagccc aattccctat ttaattcatc
     9481 tatttcatct tttggatagc ctgttactat gcgatctacc aatgtgttta agaagtgatt
     9541 atgcgcttta atccagcggg taaattcccc gcccaaatcc cattcatctg cgtaccttaa
     9601 tacgatctcc tttagggagt ccccgtttct gtctatgagc tcacaaggga ggattatcat
     9661 gcccctatcc ggacagccgc taaaagcctt aaatctttca tataaaaacg ccgttacctt
     9721 cgcgggaaac gaagtagggc agctatctgt tggcattccc tcctctacat atgcgatccc
     9781 cgcttctgtg gtgttggaca ccataaaccg catttctggc tgagcagcac agcttagaag
     9841 tcctttccaa tcctcatagg ggttgatgcc cctgctgacg gatgtaatta tccgtttttc
     9901 ctcagttatt cttcccttgt ctatcccccg aaggaggagg gtgtaaagac cgtcctgttc
     9961 gttaagtaca tccaccagcc catggggtat aggctggact accaccactt tacccataaa
    10021 caggccttgc ctgttcatcc cgtccaccat agagtcgacg aaggctctta aaaagttgcc
    10081 ctcaccaaac tggacaattc tctccggcat agcctcaagg ttacctaccc ccacatcctt
    10141 gaaaagccct gatcccttat cgatcagctc tctattgagc cttttcacac ctaaaacccc
    10201 cttaaaactt gcttctatcg gcccacaacc ccaacgccgg gttgcttata taaaagacct
    10261 cttccccgtc ttctaaagta ttgtatccat cccttaattt cttcggatcg tatttttcca
    10321 aagcctcctt cagggacata taattgtagt tgatgccctc aacttcctcc cgggtcagat
    10381 ggcccggggc gtaggtaatg gaaaacctgt catcggatga accgtggatt aaatgggctg
    10441 caacggattg gttctcctta agatcctgat tctcgttaaa caactccaac acctttaacc
    10501 tgccgacata tccatacttt cgtatcagcc tgtcattttc ctggtcctcg ccgaactgcc
    10561 tgaccccagg ggccagaact atgagctctc cgccgtcctc aatggccatc cgggtcctat
    10621 agacggattt attccccagc caggtactct taaattctgt ttcatctaaa tagaccacga
    10681 ccttttttag gggcctatcc acatactcaa gattcttttc ctggctcaat ttaacggctt
    10741 cttcaaacaa ggatctttca ttgccgatat acagtccatg gatatccaca ttatcttccc
    10801 tggtggttgt caccgtcaat acatacatta ggggcatccc cttagtaaag tgcttctcgc
    10861 aatagtcaaa gacctttcgc acaggggaat gatcccttcc catgatcctt tccatcccaa
    10921 agaccgcacc cagcatgtgg ctcttgttta tgatgtccat accgccgcag cccacaaata
    10981 tattcttgct gtagttggcc atacccacca cctcatgggg caccacctgc ccaatggaga
    11041 tgattagatc gtagctttcg tccagcagta ttttattgat ttccacatcg atgggttcgt
    11101 ccacaagccc ccctgatatt tcggatatat actgtcccgg gacctgaccg actttgacca
    11161 catccgtcct ccaattatgg actttgaagg aatcctttgg gatatcgtcc ccaaagaaca
    11221 gatcaatctc ctcgtgggtc attgccatat gggttcccag cgcgggcata atatccacct
    11281 ggcagctatc cttgagaata tcatagtaca tcccggtaat atccccggcc ccggagtggg
    11341 ccctagtcag atccggtggg atcaatagaa ccctttccag ttttctatca tgtcccttta
    11401 atgattttgt aagggccttt ctaatccttt cttcatgaag gccttggccg tcatctgaag
    11461 taatactgag tctgatcatc ccattcacct caactatatt tatcctttga ttcgtgatct
    11521 ctataccata ttcttacccc tggggtccct gtattaacac gaatatttca tgattggggt
    11581 gtcaaagcca tatcgtaact tagattttgg cctatcgatt atcataattc attgagataa
    11641 agcagattcc agaataaacc atcctcccat tcggaacaaa caaggcctct taagccgatt
    11701 ccactaatag gaggccggat tggtagcgat gggctatgat tgcattcgat actataacta
    11761 attgtcgtga acatcgccgg gcttttcaag cctatccaag ataaaaatca agaaaatgca
    11821 gataacgaat ataatgccca tcataacgaa aggcagctct tcagatacag aggccagcac
    11881 cccgcctata taaccgaagg gtgccgttac ggcaaacaga ataacgtaca ggatggacat
    11941 cgtttttgcc ctttccttgt catcaatggc atttgccaac atagattcaa taaggggaaa
    12001 ggatacggcc gtaccaaagg ccatgaacac agtcgatata atcaaaacaa aatagttctt
    12061 atgaggtgat accaccaata ggagattgct tataattaaa gatgcgaagg aaccaaggag
    12121 cggttttttt aggcttatcc ttcccagcgc gggcattaca aacaataata ccaacagcat
    12181 tattcccgat tgaatcatgg gaaagagggc tatgctcccc tgactgaatt gtagcttttt
    12241 agttaataga atcgcaaaaa agttggttct tataacggta tggatattag agaagatgga
    12301 gagtaaaaaa acaatcatag tgtagggcgt agatattaat tgcgttgcag cccttttgta
    12361 atcttccaaa ctatcaaaaa ggcccacatt tttagactcc ttcattctga tgagcccctg
    12421 cccagtctca tgaacaagat aatttctgat aatgaacatc gaggtcatgg aaaagaaagc
    12481 aaaaaagtaa aggcccctga cggcgggcac cagctgatac ctgcttacaa gaatcccggc
    12541 aacaggcgca acgaaaccgg acaatatccc cgctatctgc gtccaagtaa atacatgaac
    12601 cctgccttcc ggtggtgtat cctctacaaa aaggcaagtc caagaagtgt gcacaaccct
    12661 gacagcgctg tttataatag ctgctattaa gaaatagtaa aaattctgag ccatggccca
    12721 tataagggta ggaatggtcc aactgataat ggcaaatacc aagctggtac gctttctgcc
    12781 cagcttatcc gttatatggc cgccaaccaa tgaaaaaaac atctgaaata ttaggccgac
    12841 agatgcgatc agaccgattt gtttttccgt actccccaag gctatcatat agacagaagc
    12901 ataagtaata aaaagattat acggtatacc ccatagaggt tccatcataa tgcacgccct
    12961 ggcgtttccc ttaaagccct ttaatatgtt tcccgtctcc cgtaattgac cccttaatct
    13021 tgccccaacc ataagctagc cccccaatgt agttcccatt tttggcacaa agttgcacag
    13081 gctacaatta tagcataaaa ggcctatatt ggcgacacac aaaaattgcc cgcagaatca
    13141 caatatcata tcctgaaaat aattgacaat gtcccatagc accatatatc atatggttat
    13201 cacctaaatt ttacaaacaa acccaaggtc atcattctct ttagaaaaat ataccgagga
    13261 ggggaactct tatatgaaaa agctaaacct ggctatcatc gggcagggac gcagcggaag
    13321 ggacatacac ggcaagtatt ttctgacgga ttcggatcgg tttaaagtag tggcagtggc
    13381 agaagccctt gaggatcgca gaaacagggc cgcagaggaa tatgggtgtg atacctatga
    13441 aaactatcag gagctcttta aaagatccga tatcgatctg gtggtcaacg catccttcag
    13501 ccatatgcac gcccatacca ccattgacct tttaaaccat gggtttaatg tcttaacgga
    13561 aaagccctgc gccaagaccc cggaggaggt ccaggctatg atggacgcag cagaggcgaa
    13621 taagcgtatg tttgccgtct tccaacaatc ccgattcgca ccctattttg aaaaggtgaa
    13681 gtccgtaatg gattccggga tcctggggag aatagtgcag ataagcataa gctttaacgg
    13741 cttcgccaga aggtgggact ggcagtgctg ccaagaattc gtcgcaggca gcctttataa
    13801 caccggcccc catcctatgg atcaggctct taatctcctt gattactacg acggtacgcc
    13861 ccgggtgttc tgtaagatgg acagggccaa cacctttggg gatgccgaag actatgtaaa
    13921 ggtcatcctt acggctccgg accgccccct tattgatgta gagatttcct cgtgtgacgc
    13981 ctacccaacg tatacatata aggttcaggg gaccagaggc ggattggagg ggaacatgac
    14041 ccatataaaa tggagatact atgacgaaag ggtggccccc aaaagggaac ttataaagac
    14101 gcccctgatg gacgaccagg gtcttcccat gtactgcggc gaaaaactgt actgggccgg
    14161 ggaaacatgg agcgtcgagg accccggggt atttgaccac gcggtaaaac ggttctatga
    14221 caacatctat gaccacctgc ttaatggaac accccttgta gttacgccac agcaagttaa
    14281 gcagcagatc catgttataa atgaatgcca tgagcaaaac ccactgtcaa aattctgtta
    14341 gttagaaa
//
LOCUS       DASRNX010000032         4494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_58833,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000032 DASRNX010000000
VERSION     DASRNX010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58833"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFD89_01200"
     CDS             <1..280
                     /locus_tag="VFD89_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56842.1"
                     /translation="TYNMRDEVRHHILISDQTLGGRDKYFIIYNPIGTLERYGVHNDV
                     GTDIYKTEGVIIRCAQKCERPLFSPLYYGESLPGLPQKELWKASDVNG"
     gene            273..1487
                     /locus_tag="VFD89_01205"
     CDS             273..1487
                     /locus_tag="VFD89_01205"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZJ56843.1"
                     /translation="MDEILRELKDHIKNQTIINTHSHHLKDDSFQGMNLDAVLRQSYV
                     NWYKVPLESTYRSRENFLDKNRHNSYFVWLQKALQELYEFSEPLSAENWDGVSRRIEE
                     AHRYEGHHMGILREKCQYKKIILDTYWHPGSNNGHPDIFTPTFRVDPLLYGYSKDARD
                     HDGNSPAELYGDIPHDLDEYVSLIKNIIALKKNKGCIALKSAIAYERPLNFKRVSRDR
                     AYGALVKEDADRTLEDIRAYQDYLFVQICKIAAELDLPIQCHTGMGQLTGTNAMALKD
                     VIDSNPETKFVLFHCSFPWMDDVNALLRTYDNVYPDLCWLPILSTSAAIRMLHELIEG
                     GTSDKICWGCDTWTSEESYGALLAFRFVLAKVLRDKVGDGYFTLEDAKYVIDNIMYQN
                     ASKLYGLARQID"
     gene            complement(1620..2006)
                     /locus_tag="VFD89_01210"
     CDS             complement(1620..2006)
                     /locus_tag="VFD89_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56844.1"
                     /translation="MKEEIKKILKMIEEGQITADEGERLIEAMGTDNLGLSEYSAQTP
                     HNNPRFLKVRVAEEGKNKVNVSIPISLVDVALKIGAKIGPKFVPEVEVLQEIDFDELM
                     QAIREGVRGKLVDIDDGGNRVEVFVE"
     gene            complement(2033..2404)
                     /locus_tag="VFD89_01215"
     CDS             complement(2033..2404)
                     /locus_tag="VFD89_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HZJ56845.1"
                     /translation="MSYPAINKCSVCGQELKITCLHCTKCDTQYQGDFHFDKFSYLNT
                     QQKQFIEIFLRCRGNIREVEKELGISYPTVRSRLDDVIRALGYELPQENPQINQKEII
                     DSLSKGEISYDQAMKMMKSKD"
     gene            2825..3661
                     /locus_tag="VFD89_01220"
     CDS             2825..3661
                     /locus_tag="VFD89_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZJ56846.1"
                     /translation="MRLGGGILRSYNNPEEWIKIVKELRYGAVVAPMKHDDPEELIDS
                     YVEEAKKADVVIAEVGAWSNPLSMDDNVRMEAIAHNKNQLNLADRIGARCCVNISGTK
                     GEVWDGAYRENYEPDTYALIVDTVREIIDAVKPKNTFYALEPMPWMYPDSPDSYLKLI
                     KDIDRKEFAVHLDTVNMINSPQRYLFNDEFIKECFKKLGPYIKSCHAKDVIMSNQLTT
                     MIKEVAPGKGTFNYPVFVREVERLNPDMPVMIEHLNTQEKYIEAFEYIEGVAKENGVS
                     LR"
     gene            3798..>4494
                     /locus_tag="VFD89_01225"
     CDS             3798..>4494
                     /locus_tag="VFD89_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZJ56847.1"
                     /translation="MFDDILQLLHQNISHRIWFISDLQQSIPENAARCMQQGIRDFRQ
                     LGLDCQRIIYLGDAVEGAHPGRLENMTKMQIGELDGLDIPVRYVVGNHDFDYFRHSKR
                     PLEGVRLPFYEAMKAIPGWKSTDSIEDFYFIEEFDDYALVYLSDHAAVDGSWYTIHGK
                     IHGNASLYPYDDAYFEELRDKIAGIEKPVFTASHYAFPGGNRPSPLLKRLLPLPNNVR
                     VHFYGHAHIGDRVW"
BASE COUNT         1338 a          879 c         1029 g         1248 t
ORIGIN      
        1 gacgtataat atgagagatg aagtaagaca ccatatatta atatcggatc agacccttgg
       61 tggacgagat aaatacttca tcatatataa tcccattggc actttggagc ggtatggggt
      121 gcataatgat gttgggacgg acatctacaa aacggaaggg gttattatcc ggtgtgccca
      181 aaagtgcgaa agaccgttat tttcacccct atattacgga gaaagcctgc ctggcctgcc
      241 ccagaaagaa ttatggaaag cgagtgatgt taatggatga aattttaagg gaattaaaag
      301 atcatatcaa aaaccagact attatcaata cccattccca tcatcttaag gatgattcct
      361 ttcagggtat gaatcttgat gctgtgctta gacagagcta tgtcaactgg tataaggtac
      421 cattggaaag cacataccgg agcagagaga attttttgga taaaaataga cacaattcat
      481 attttgtatg gcttcaaaag gccttgcagg agctgtacga attcagtgag cccttgtccg
      541 ccgagaactg ggatggggta tcaaggagga tagaggaggc tcataggtat gaggggcatc
      601 atatgggcat cctccgtgaa aaatgtcagt ataagaaaat cattttagat acctactggc
      661 accccggcag caacaatggg catcccgata tatttacgcc taccttcaga gtagacccat
      721 tgctttacgg gtatagtaag gatgctcggg atcatgatgg caatagcccc gcggagctct
      781 atggagatat cccccatgat ctggatgaat atgtatccct tattaaaaac attattgccc
      841 taaagaagaa taaagggtgt attgccttaa aatctgctat agcctatgaa agacccttga
      901 attttaaaag ggtgtctagg gatagggcct atggggcatt agttaaggaa gatgcagata
      961 ggacattaga ggacatcagg gcttatcagg attatctatt tgttcagata tgtaagatcg
     1021 cagccgagtt ggatttgccc attcaatgcc ataccggcat gggacaactt acaggtacaa
     1081 atgctatggc ccttaaggat gttatagaca gtaatcctga aaccaagttc gtattgtttc
     1141 attgtagctt tccgtggatg gatgatgtca acgcactact tcgtacatat gacaatgtat
     1201 atccggattt atgctggctg cctattctct ctacgtccgc cgctattaga atgctccatg
     1261 aactcataga aggcggaacc tcggataaga tctgttgggg atgtgatact tggacatcgg
     1321 aggaaagcta tggtgctttg ctggctttta ggtttgtatt ggcaaaggtc cttagggaca
     1381 aagtagggga cggctatttt acgctagagg acgctaaata tgtgatcgat aatataatgt
     1441 accaaaacgc atcaaagcta tatggattag caaggcagat agattagtgg gctctatctg
     1501 ccttgtttgc ataatcgaaa taatgggagc aataaagcgg caagggttgt cgacgagaga
     1561 agcagcactg aaacctctgg cttctggagc tttctagtat accatttacc ttaattctac
     1621 tattccacga aaacttctac cctattaccg ccgtcatcaa tatccactaa cttaccccgg
     1681 acgccctccc taattgcttg cattagctca tcaaaatcaa tttcctgcaa gacttccact
     1741 tcagggacga atttgggtcc gatcttagcg ccgatcttaa gagctacatc gaccaaggaa
     1801 attggaatac tgacattaac cttgtttttc ccctcctcag cgacccgaac ctttaagaat
     1861 ctggggttat tatggggtgt ttgcgcacta tattcagata aaccaaggtt atctgtgccc
     1921 atagcctcaa ttagcctttc gccctcatcg gctgtgattt gaccttcctc gatcatttta
     1981 aggatctttt tgatctcttc tttcataatt agcacccttc cttttatttg atttaatctt
     2041 tgctcttcat cattttcatg gcttgatcat agctaatctc acccttactc aacgaatcta
     2101 taatctcttt ttggttaatc tggggatttt cttggggtaa ttcataccct aaagcgcgga
     2161 tgacatcgtc tagccttgac cttacggtag gatatgaaat accgagttct ttttctactt
     2221 cacggatgtt gcccctacat cttaaaaaga tctcaataaa ttgtttttgc tgggtgttta
     2281 ggtagctgaa cttatcaaaa tggaagtcac cttggtattg ggtatcgcat ttggtacaat
     2341 ggagacaagt tatctttagt tcctgcccac atactgaaca tttgttaata gcgggataag
     2401 acaagcgcaa tccctccttc atagtttatg gggaattaac ttcttattat aactataacc
     2461 caaatataaa atatgtcaat gttaaaattg aaataattaa tatataaagt ctaatacatt
     2521 aaattattta atattaaaat caattacatg agaaagaaca caagaatacg gctcctctgt
     2581 gcgcggctta gtcatttcct acaataaaca aaatatcatt gagaaaaggg tagcgatgga
     2641 tgtataatag attggaaggt atatagttcg tacataaatg agatagaggt tgtcaatgtg
     2701 ttgtccaagc gtcattgaag gtatgttatg gggaagatca tctgatatcc ggacaaatca
     2761 aggttaagca gcgatttata tttttaaaga gctaatcaaa gattatagag taaggaggat
     2821 ttttatgaga cttggtggcg gtattttaag gagctataac aatcccgagg aatggataaa
     2881 aatcgtaaag gaattaaggt atggtgccgt tgttgcaccg atgaagcacg atgaccccga
     2941 agagcttata gactcatatg tagaggaagc aaaaaaggcg gacgtggtga tagcagaggt
     3001 tggcgcatgg agcaatccct tatccatgga tgataatgtg cgcatggagg ccatagccca
     3061 taataaaaac caattgaatt tagccgatag gatcggtgcc aggtgctgcg tcaacatatc
     3121 cgggacaaag ggagaggtat gggacggtgc ctatagggag aactacgagc cggatacata
     3181 tgcgttgata gttgataccg tgcgggaaat catagatgct gtaaagccaa agaacacctt
     3241 ctatgcccta gagcccatgc cctggatgta tccggattca cccgatagct atctcaagct
     3301 tataaaggat atagaccgaa aggaatttgc cgtgcacctg gatacggtaa acatgatcaa
     3361 cagcccccaa aggtatctat tcaacgacga atttatcaag gaatgtttta aaaagctggg
     3421 accttacata aaaagctgtc atgccaagga tgtaataatg agcaaccaat tgaccactat
     3481 gattaaggaa gtagcccccg gcaagggtac attcaattac ccggtatttg taagggaagt
     3541 ggaaaggctg aaccccgaca tgccggttat gattgagcat ctaaataccc aggaaaaata
     3601 tattgaagcc tttgaatata tagagggcgt agccaaggaa aacggcgtga gcctgcgtta
     3661 aataccaaga aaacactgaa cacagggtga cgacacaagg ggacggttcc tctgtgttgt
     3721 cactatgcat gcgcacatag gaaccgtccc cctgtgtccc tgggtttaca tccatatgct
     3781 tttgaaggag aaaatgcatg tttgacgata tattacaact tttgcaccag aatatttccc
     3841 atagaatatg gtttatttcc gatctgcagc aatccattcc ggaaaacgct gcccgatgta
     3901 tgcaacaggg gattcgggat tttcggcaac tgggattaga ctgccaacga atcatatatc
     3961 taggggatgc tgtagaaggc gctcatcccg gacgtttaga gaatatgacc aaaatgcaaa
     4021 ttggggagct ggatgggctc gatatcccgg ttcggtatgt ggtaggaaat cacgattttg
     4081 attacttcag gcattccaaa aggcccctag aaggggttag gttgcctttt tatgaggcga
     4141 tgaaagccat tcccggttgg aaatctacgg actctataga ggatttttat tttattgagg
     4201 aattcgatga ttatgccttg gtatacctca gcgatcacgc agccgtggac ggcagttggt
     4261 atacaattca cggaaaaatt cacgggaatg cctcccttta cccatatgat gatgcatact
     4321 tcgaagaact tagagataag atcgcgggta tcgaaaaacc ggtattcacg gcgtcccact
     4381 atgcttttcc gggcggaaat cgtccttcgc cgttacttaa gcggctgttg ccattgccca
     4441 ataatgtaag ggttcatttc tatggtcatg cacatattgg ggatagggtg tggg
//
LOCUS       DASRNX010000033        11989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_696078,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000033 DASRNX010000000
VERSION     DASRNX010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11989
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696078"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1960)
                     /locus_tag="VFD89_01230"
     CDS             complement(215..1960)
                     /locus_tag="VFD89_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HZJ56848.1"
                     /translation="MRILLTTLNSKFIHSNLALRYLKDSLNHLPITSITDEFTINDHM
                     EKIVGNIYRHRPDIIGLSCYIWNIEETLMVVESLKEVLPDLIVILGGPEVSFDSVSLM
                     EGSPKIDIIVKGEGEVTFPLLIERLISDKPLDHVEGLTFRKAGAIKENPDREPIRDLD
                     TIPFPYGDDFEDLDNRIIYYETTRGCPFQCQYCLSSVSQGVRYLSLDRVKEDIKTFID
                     AGIPQVKLVDRTFNCNPNRAREIFEMIMEVGGNTNFHFEMCGDLLDAETLDLLKDAPP
                     GLFQFEIGVQSTREETLEIIKRKTDFDRLSERVKTLRGYRNIHLHLDLIAGLPGEDYL
                     SFRNSFNDVYELRPDRLQLGFLKLLKGSGIRINAQDWGYKFLSHTPYEVLENRDISYD
                     QVLVLKDVEELVEKYYNTHRYTNSLEYLGRHFGGDYYALYEDFAGYWRDRGYTELSHS
                     LIRHYEILVEYGLGIDGVDGDYFKDLVRLDYASQGKPSRYPEGIDVVIDEEKKEWVRS
                     FFNNRENISRYLPHLAQYTPSQISRMAHIEFFDCEVAGNKDTSGSDRNPTIILFDYNI
                     SNKLFKKSKLTRLKA"
     gene            complement(1957..2592)
                     /locus_tag="VFD89_01235"
     CDS             complement(1957..2592)
                     /locus_tag="VFD89_01235"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZJ56849.1"
                     /translation="MTIIDISMPIRLDMQVYKNRDEKRPDIKVMRDFDSGSVYESRLS
                     IDMHTGTHIDAPLHMIEGGEDLTVYPIERSVSPCRVLDFTHIKDGITGDDLSDKEILP
                     GEFILLKTQNSLEEDFNPDFIYLERTGAEYLQKKGVTGVGIDSLGIERSQPGHETHKT
                     LLRNRIMILEGLRLKGVAEGAYILIAAPLNIPGVEASPVRAFLMDRGAFNL"
     gene            complement(2579..4078)
                     /gene="zwf"
                     /locus_tag="VFD89_01240"
     CDS             complement(2579..4078)
                     /gene="zwf"
                     /locus_tag="VFD89_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004404007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZJ56850.1"
                     /translation="MTIEALLVVFGGTGDLAHRKLYPAVYNLYLSGLLPENFALVSVG
                     RRDKSVEEFRSEAMESIKEYSRSEVLDTDGLEKVLSGFYYYTKNFYDDQGYERLGEFL
                     SELDGRYGTRGNRIFYLAVAPEHFGPIVERIDAAGLAPNEKGSWQRVVIEKPFGKDLE
                     SASRLNEDITKAFSEDHIYRIDHYLGKEMLQNIMVIRFANTLFEPLWNNKHIDHVQIT
                     SCETIGIENRGGYYEGSGALRDMMQNHMLQLLMLTAMEPPVNLDTESIRDEKVKVLKA
                     LSPMTAEEVARNVVRGQYGGGVDPESGDGVIGYRQEDRVFEDSDTETYVGMKLMVNNI
                     RWAGVPFYLRTGKRLPCKTTEIAIEFSKPPEVLYFKEFQNLQSNLLVIKIQPREGAFL
                     QFNAKKPGARSEIIPVAMDFCQNCTIDAVSAEAYERLLYDVLRGDSTLFTRWDEVEHS
                     WRFIDGIAGAWDKTSPAFPNYSAGGWGPDEARELIERDGRIWRDVCDYY"
     gene            complement(4285..5310)
                     /locus_tag="VFD89_01245"
     CDS             complement(4285..5310)
                     /locus_tag="VFD89_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZJ56851.1"
                     /translation="MNRKKWTLVYIVLNILLIALIGVLDPEIKDVGKIFRDVRLSWIT
                     GGVLLMLIFWLMDGIILSYAVKVISRTKAFWGCLRVSLIGQYYNAVTPFASGGQPAQV
                     YYMGKLGVSGGSASSVLMIKFLIYQVVLSLYCLAAFIWKGITIYNHNPWVFWFSVVGF
                     IINAGAVVLLVSLSFNKGFVEGLVFKTIDILGAIRLIKDTHGMKERLISHVEDFHEGL
                     KFMRGNARAILTLGMMTAIQLICFFSITFFIYRALGLEGEKWINVVSVQSLLYLAVSF
                     VPTPGSMGASETGYIAFFRLFFPGDLIFVSMLLWRAISYYLNILAGVIIILLDSLRQI
                     VSHDVAN"
     gene            5590..5766
                     /locus_tag="VFD89_01250"
     CDS             5590..5766
                     /locus_tag="VFD89_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56852.1"
                     /translation="MKSISVSLLEAALAEREEYLEELKRIEGGLQEQISNLQYEIDEL
                     LIRIYDSYKKGSKG"
     gene            5970..6524
                     /locus_tag="VFD89_01255"
     CDS             5970..6524
                     /locus_tag="VFD89_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZJ56853.1"
                     /translation="MSEQIKQIAMRIKELRVIHDLTQQELAEHLSLDVETYRCYEDGA
                     TDIPVSILYGIANRFNVELTAILTGDEPKLRLYSLVRKGKGIKVNRRKEYGYQSLAYN
                     FVHKKAEPFLVTVKPDSEDTPIAQNSHLGQEFNYVLEGTLKISIDGHELVLEEGDCVF
                     YDSNSKHGMKALNDKPAKFLAVIM"
     gene            6541..8190
                     /locus_tag="VFD89_01260"
     CDS             6541..8190
                     /locus_tag="VFD89_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZJ56854.1"
                     /translation="MLKHFVKDEFNSYEDFWNKFNVEVPDNFNFAYDVADRLAQIKPD
                     KPAIVWYGDNGETKIFSFKDLKYYSDKAANFFMDSGIKKGDPVMLILKRRYEFWFCLL
                     ALHKLGAICIPATHLLTGNDIVYRNNAASVKMIVSVSEDDVINHIEASEEKSPSLVTK
                     VSVGGTYEGWLDFDSGMKNASEVFHRPQGEAGTSNGDISLLYFTSGTTGYPKMVQHNF
                     TYPLGHILTAKYWQYVIDGGLHLTIADTGWAKAMWGKIYGQWIGGSAIFVYDFERFNP
                     HKMLETIEKFKVTTFCAPPTMYRFLIKEDISKYNLESLKYCVVAGEPLNPEVYHQFYN
                     ATGIRLMEGYGQTELTITLATFPWMKPKPGSMGKPSPGYDVDIIDEEGQSCGIGEEGQ
                     IIIRTDKRKPVGMFDGYYRDEAKTHEVWRDGIYYTGDMAWRDEDGYYWFVGRADDVIK
                     SSGYKIGPFEVESALIEHPAVLECAITAVPDEIRGQVVKATVVLAKGYTASDDLKKEL
                     QDHVKRVTAPYKYPRIIDFVKELPKTISGKIRRVEIREQDN"
     gene            complement(8515..9585)
                     /locus_tag="VFD89_01265"
     CDS             complement(8515..9585)
                     /locus_tag="VFD89_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZJ56855.1"
                     /translation="MNIIRSGRTRDLWDNSISEAEISAKKKANILIVEDEPITANLLQ
                     KMLMGQGYRVVGIESTGEKALDKISLQNPDLILMDIQLGGSLDGIEVAQVVHKEFDLP
                     LVYITSHADDDTLGRAKITEPFGYILKPINPRELNMVIEIALYKHGMERKIREDNERT
                     KRILEKAFFDLTKTVCRTLGFRDAYTAGHQERVAKLAAIVGRRMGLGQDDINELYIGG
                     LLHDVGKIGIPTQILNKMPPLTDTELTLIREHPRIGFEILSDAELPWNIRDIVLNHHE
                     KLDGSGYPSGLSGCQISINVRIITVCDVAEAMSTLRPYRAARTKDKVIRELSRGRGIK
                     YDANVVDVVLDLIETGEFNPWK"
     gene            complement(9648..11882)
                     /locus_tag="VFD89_01270"
     CDS             complement(9648..11882)
                     /locus_tag="VFD89_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZJ56856.1"
                     /translation="MFIQMDGRIIEANEAAVKAYGYSREELLSMSIFRLRGGDENALV
                     ENQMAMAGDTGILFEGVHARKDGSSFRVEVSSQGVTIDNKRLLLSVIRDITERSKMMD
                     ELRDAKDEAETANRAKGEFLARMSHEIRTPMNGVLGMTELALSTELNNEQWEYITMAK
                     ESALSLLQIINDILDFSKIEAGRLEMDNSPFALNEIVGKVCDTLALRAHEKNLELMYY
                     IDPDVPLNLVGDGVRLQQVLYNLLGNSVKFTDTGEISLDINMVKELDTSVMLSFEIRD
                     TGIGIPGDKMDRLFQSFSQLEDTHTRKYGGTGLGLSIAKQLVEMMGGRMDVKSRVGEG
                     SIFTFTAYFAKGEQMIADPDSMVDISCLRILVVDDNRANRIILEKILKRRGAYVASAC
                     DGVTAIQLMTEYLEDNIPIDIILLDGHMPGMTGFEVAEHIKNHTGLKDTTIMMLTSME
                     IKDGMTKCKEMGIEAYLIKPIGEEELLDAISKTIHHKHTAHKETDGSIAVFREDQKGR
                     RHGLGRVLIAEDNIINQKLATALLEKRGYSAISVSNGLEAIETIRDGIEFDMILMDVQ
                     MPEMNGLEATRRIREMEQITGNHIPIIAMTAYAMKGDREKCIDAGMDGYVSKPLNPEV
                     LYDILDRFSKPKIPVDLSGLNLTTGHDEKFVKELIRTLIDSYPKNLEEIANAISQRDP
                     KKLEKSAHGMRGAVSNFGAHHAVELLTQLGGLASEGRTEGAMGILDELRNEMDRIRIY
                     LEDG"
BASE COUNT         3201 a         2997 c         2441 g         3350 t
ORIGIN      
        1 tatatgcatt gattttatgt gtaaatatga ttacaattct gacaaatcag gccccaatag
       61 tcttcgacag ccttagaagc agcaatggta ttaatagaat ggatcgatag aataaatcgg
      121 tagaatagaa tatctttaaa aaatggacag tcctatgact gcccattgaa ggaaaataac
      181 cgtctcaatg cttctaaaag cgtgggaatc atccctacgc ttttagtctg gttagcttgc
      241 tcttcttgaa gagcttattt gaaatgttat aatcgaagag tattattgtg ggatttctat
      301 ctgacccgct agtatcctta ttcccggcga cttcgcaatc aaagaactct atatgggcca
      361 ttcgcgaaat ctggctgggg gtgtattgtg ccagatgcgg aagatatcta gatatattct
      421 ccctattatt gaaaaaactt cggacccatt cttttttctc ctcatcaatc actacgtcta
      481 tcccctcagg atatctagaa ggctttcctt gggaggcata atcgagcctt accaaatcct
      541 tgaagtaatc gccgtctacc ccgtctatgc ccagcccata ttcaaccagg atttcataat
      601 ggcgtataag gctatgggac aattcggtat aacccctatc cctccagtat ccggcaaagt
      661 cttcgtacag ggcgtaataa tctcccccaa aatgtcgccc taaatattcg aggctgtttg
      721 tgtagcgatg ggtattgtag tacttctcca ctaattcctc tacatccttt agcaccagca
      781 cctggtcata ggagatatca cggttttcta gtacctcgta gggcgtgtgg gatagaaatt
      841 tataccccca atcctgggca ttgattctta taccggagcc cttcaggagc tttaaaaagc
      901 ccagttgaag cctatccggc ctcagctcgt aaacatcgtt aaaggaattt ctaaaggata
      961 ggtagtcttc cccgggcaga cccgctatca aatccagatg aagatggata ttcctgtatc
     1021 cccggagtgt ttttaccctc tccgacagcc tatcaaaatc cgtcttccgc tttataatct
     1081 ccagggtctc ctcccgggtg gattgaaccc ctatctcaaa ttgaaagagt ccggggggcg
     1141 catcctttaa aaggtcaagg gtttctgcat ccagaaggtc gccacacatt tcaaaatgga
     1201 aattcgtatt cccgcccacc tccattatca tctcaaagat ctccctggct ctattgggat
     1261 tacagttaaa ggtcctgtcc accagcttga cctggggaat gccggcatct ataaaggtct
     1321 ttatgtcctc cttcacccta tcaagggaaa gatacctaac cccctgactg acggaggaca
     1381 gacagtattg acactgaaag gggcaacccc tggtggtctc atagtagatt atccggttat
     1441 ccagatcctc aaaatcatcc ccatatggaa aggggatcgt gtccaaatcc cttatgggtt
     1501 ctctgtccgg attctctttt atcgccccgg cctttctaaa ggttaatccc tccacatggt
     1561 ccaggggttt atcggagata agcctctcaa taagaagggg aaaggtgacc tccccctccc
     1621 ccttcactat aatatcaatt tttgggcttc cctccatcaa ggaaacgctg tcaaaggata
     1681 cctcgggccc ccccaagatg acgattagat ccggcaatac ctcctttagg gattccacta
     1741 ccatcagggt ttcctcaatg ttccaaatat agcaggacag gccgattatg tccggcctat
     1801 gcctgtatat attccccacg attttctcca tatgatcgtt tatagtaaat tcgtcggtaa
     1861 tggaggtgat aggcagatgg ttcagactgt cctttaaata cctaagggca agattgctgt
     1921 gtataaactt agagttcaag gtggttaaca gaattctcat aggttgaatg cgcccctatc
     1981 cattaagaac gcccttacgg gagaagcctc caccccaggg atatttaggg gtgcagcgat
     2041 tagaatatag gcgccctcag caacgccttt taatctcaga ccctctagga tcattatcct
     2101 gtttctcaaa agggttttat gggtctcatg gccgggttgg ctgcgctcta tccccaaact
     2161 gtctatgccc acacctgtaa cgcccttttt ctgtaaatac tccgcccccg tcctttccag
     2221 atatataaaa tccggattaa aatcctcttc aagggaattt tgagtcttta agagtatgaa
     2281 ctccccgggt aaaatctcct tatccgagag atcatctccc gtgatgccgt cctttatatg
     2341 ggtgaaatcc aataccctgc aaggagacac cgacctctct atcggataga ccgtaaggtc
     2401 ttccccaccc tcaatcatat gaagcggggc atcaatatga gtcccggtat gcatatcaat
     2461 agataatcta ctctcataga cacttccgct gtcaaagtct cgcataactt taatgtcagg
     2521 tcgcttttca tccctgttct tatatacctg catatccaaa cgaatcggca tcgatatatc
     2581 aataatagtc acagacatcc ctccatatcc ttccgtccct ttctattagc tccctcgcct
     2641 cgtccgggcc ccagcctcct gcgctgtagt tgggaaaagc cggcgaagtc ttatcccagg
     2701 cccctgctat accgtcgatg aacctccatg aatgctcaac ctcatcccat cttgtgaaga
     2761 gggtggaatc ccctctcaga acgtcataga gtaacctctc gtaggcttcc gccgaaaccg
     2821 catcgatggt acagttctgg caaaagtcca ttgccaccgg gatgatttcg ctgcgggctc
     2881 cgggcttttt tgcgttaaac tgaagaaacg ctccctccct aggttgaatc tttatcacca
     2941 ggaggttgga ttgaaggttc tgaaattctt taaagtataa tacttccggc ggtttagaaa
     3001 attctatggc gatctccgtc gtcttacagg gaagtctctt cccggtcctt agataaaaag
     3061 gtaccccggc ccatcgaata ttgttgacca ttaatttcat gcccacatag gtttccgtat
     3121 ccgagtcctc aaaaaccctg tcttcctgcc ggtatccaat cactccatcg ccgctttccg
     3181 gatccactcc cccgccatac tggcccctga ctacatttct ggctacttcc tccgccgtca
     3241 tgggagataa ggcctttaag accttaacct tttcgtcccg tatggattcg gtatctaaat
     3301 taaccggcgg ctccatagcg gttaacatga gaagctgcag catatggttt tgcatcatat
     3361 cccttaaagc tccggaaccc tcatagtatc cgcccctgtt ctctatccca atagtctcac
     3421 aggacgttat ctgcacatga tctatatgct tgttattcca caggggttca aagagggtgt
     3481 tggcaaaacg tataaccatg atgttttgaa gcatttcctt gcctagatag tgatcaatcc
     3541 tatatatgtg atcttcgcta aaggcctttg tgatatcctc gttgagcctg gaagccgatt
     3601 caaggtcctt gccaaagggc ttttctatga ccaccctctg ccatgaccct ttttcgttgg
     3661 gtgccaggcc ggcggcatcg atcctctcaa ctatgggacc gaagtgttcg ggggcaactg
     3721 ccagataaaa aatcctgtta ccccgagtcc cataccttcc gtccaattca cttaagaatt
     3781 cgcccaacct ttcatatccc tgatcatcat aaaaattctt cgtataataa tagaatcccg
     3841 agagaacctt ttccagtccg tccgtatcta aaacctcact tcgcgaatac tccttgatgg
     3901 attccatggc ctcggatcga aattcctcaa ccgatttatc ccttctgccc acggatacca
     3961 aagcaaagtt ctctggcaat aatcccgaca ggtataaatt ataaacggcg gggtaaagct
     4021 ttctatgggc caggtcaccc gtccccccaa aaactaccag caaagcctct attgtcatcg
     4081 cctcctacag aaagtccaac tcaaaataag ccaagtttac atatttatac ccatttcggc
     4141 cccctgtaaa ccgtaatgca tatacagata aaaattccaa ataatattat acgctaacat
     4201 ttatcatcta tgggtaaatt cagttgaccc attatctatc catatcatat gttccggtcc
     4261 attttttaaa cggtcccaag cacatcaatt ggccacatcg tgggagacta tctgcctcag
     4321 gctgtccaac aatataatga taaccccggc caaaatattt aaatagtagc tgatagctct
     4381 ccacaagagc attgaaacaa agatcaaatc gcccgggaag aagagcctaa aaaaggctat
     4441 atagcccgtt tccgatgccc ccatggaacc cggagtcggt acaaaggata ccgccaggta
     4501 gagaagggat tggactgaca ctacgtttat ccatttttcc ccctcaaggc ctaaggccct
     4561 gtagataaag aaggtaatgc taaaaaagca gattagctgt attgcagtca tcatgcccag
     4621 ggtcaatata gcccttgcgt ttccccgcat gaacttaaga ccctcatgaa aatcctcaac
     4681 atgggagatg agtctctctt tcattccatg ggtatccttt ataagccgaa tagcgcctag
     4741 aatatcgatg gttttgaaga ccagcccctc cacgaagccc ttgttaaagg acaatgagac
     4801 caacaggaca accgcccctg cgtttataat aaacccgaca acggagaacc aaaaaaccca
     4861 gggattatgg ttgtagatgg ttataccctt ccagatgaaa gcggcaagac agtataatga
     4921 gagtaccacc tggtatatga gaaatttgat catcaaaacg gaacttgcgg agccgccgga
     4981 tacccccaac ttacccatat aatatacctg ggcgggctgg cccccgcttg caaaaggcgt
     5041 gaccgcatta tagtattgcc ctataaggga gaccctgaga catccccaaa atgccttggt
     5101 ccggcttatt accttaaccg cgtagcttaa aataatcccg tccataagcc aaaatatgag
     5161 cattaagagg accccgcccg ttatccagct aagcctgaca tctcggaata tcttgcctac
     5221 atccttaatt tcgggatcga gaacgcctat tagtgcaatc agtaatatgt tcaaaacaat
     5281 gtatactagt gtccattttt ttctattcac gattactccc cctcggacga gctagataga
     5341 tattatcact aaagtatcct ataatcaata tacccccggc aaaccccttt gggcccatgt
     5401 tgtgatgcaa ttaccaatgc aattgtttcc gatgttttaa tggagtgcct cgtccggagc
     5461 gccggaccgt cgctgaaatt caaaatagct cataggttta tatcgatacg catagatata
     5521 tagggataga tgtttagcta ttatgattaa gggtataaat agctaacaat aaaaggggga
     5581 cggtcaatta tgaaaagtat aagtgtaagc cttcttgaag ccgcccttgc agagagagaa
     5641 gaatatctcg aagaactaaa gaggattgag ggaggtcttc aggaacagat aagcaatctt
     5701 cagtacgaaa ttgatgagct tctcattaga atctatgatt catacaagaa aggaagtaaa
     5761 ggttaaaggc gatagcgtac ctataaatat ttcaaccttt tgtgggccct gttcttcggg
     5821 atggggccca tgtttattgc cttgattttc gctattcact tctatatact gtaaacatcg
     5881 aaccttcggt cggacaggct attaggctat gcccaaccga cgatctttag ggcattttag
     5941 ccagaactat tatttgaggg ggagtacaga tgtcagaaca gataaaacaa atcgctatga
     6001 gaataaaaga attgcgggta atacatgacc tgacacagca ggaattggct gagcatttaa
     6061 gccttgatgt tgagacatac cgctgttatg aagatggtgc cacggatatt cctgtaagta
     6121 tcctatacgg catcgcaaat cgcttcaatg ttgagcttac cgctattttg accggtgacg
     6181 aacctaaatt aagactatat tccctggtcc gcaagggtaa gggcatcaaa gttaacaggc
     6241 gcaaagaata cggctatcaa agcctggcct acaactttgt gcataaaaaa gcagaacctt
     6301 ttttggtaac cgtcaaaccc gattcggagg atacacccat tgctcaaaat tcccatctgg
     6361 gtcaagagtt taactatgtg cttgagggca cccttaagat cagcatagac ggccatgagt
     6421 tggtcttgga agaaggcgat tgcgttttct atgactccaa cagcaaacat ggaatgaagg
     6481 ctttgaacga caaacctgct aaatttctcg cagtcatcat gtaaaggagg acctgttccc
     6541 atgctaaagc attttgtaaa ggatgaattc aattcttacg aggatttttg gaataagttt
     6601 aatgtagagg taccggataa ctttaacttt gcctatgatg tcgcggatcg tctggcccaa
     6661 attaaaccgg acaagcccgc catagtatgg tacggcgaca atggagaaac caagatcttt
     6721 agctttaaag accttaagta ttacagcgac aaagccgcca atttctttat ggattcgggc
     6781 ataaaaaagg gagatcccgt tatgctcatc ctcaaacgaa gatatgaatt ttggttctgt
     6841 cttttggccc tacataaact gggtgccata tgcatcccag ccacccatct cctgaccggt
     6901 aatgatatag tctatagaaa taacgccgcc tccgtaaaga tgatagtatc cgtcagcgaa
     6961 gatgatgtga taaatcatat tgaagcatct gaggagaaat ccccctccct ggtaaccaag
     7021 gtttcagtag gcggcacata tgagggttgg ctggattttg attcggggat gaaaaacgcc
     7081 agcgaggtat ttcatagacc ccaaggggaa gcgggaacca gtaacgggga tatctcactc
     7141 ttgtacttta cctcggggac taccggttat ccgaagatgg ttcaacacaa cttcacatac
     7201 ccgctgggac atattttaac cgctaagtat tggcaatacg tcatagacgg cggtctccat
     7261 ctaaccattg ccgatacagg atgggccaag gctatgtggg gcaagatcta tggtcaatgg
     7321 atcggcggca gcgccatatt tgtatacgat tttgaaagat ttaatcccca taagatgctg
     7381 gagaccatag agaaatttaa ggtaacaacc ttttgcgctc ctcctaccat gtatagattc
     7441 ctcataaaag aggatatctc caagtacaat ttagaatccc taaaatattg cgtggtagcc
     7501 ggtgagcccc tcaatcccga ggtatatcac caattctaca atgccacggg aatcagactt
     7561 atggaaggtt acggacaaac ggaattgacc atcactttag ctacattccc ctggatgaag
     7621 ccaaagccgg gttccatggg caagccttcc ccgggatacg acgtggatat aatagatgag
     7681 gaaggccaat cctgcggtat aggtgaggaa ggccaaatta tcatccgtac ggataagcga
     7741 aagcctgtcg gtatgttcga cggttactat agggacgagg caaaaaccca tgaggtatgg
     7801 agggacggta tctattatac gggggatatg gcatggcgtg atgaggacgg ttattattgg
     7861 tttgtgggca gagccgatga tgtaatcaag agctccggat ataagatcgg acccttcgag
     7921 gtggagagcg ccctcataga gcatcccgcc gtattggaat gtgccatcac ggcagtcccc
     7981 gatgagattc gcggccaagt ggtaaaggca actgtggttc tggccaaggg ctatacggca
     8041 agtgatgatc ttaaaaagga gcttcaggat catgtgaaaa gggtgactgc accctacaag
     8101 tatccacgga ttatcgattt cgtcaaggag cttccaaaga ccataagcgg aaagatccgc
     8161 agggtggaga ttagagaaca ggataattga gtcccattga accattacat gaaataggtg
     8221 ttcaatagcc gttgctgata tatgtacact tcggaaaaat caatattcgg tataagaaat
     8281 ataagcgagg aactgctcgg tcagttcctc gctttttgta caagcaccac gaatccaata
     8341 ttccgtaaat tacctttatc ccctagactg gcttttctga taacaaatag gctgccttgt
     8401 atcgtcccat aaaaaccttt cgtgtcgggt ttcagcaaaa acctatcccg ccggtatctt
     8461 tccacattgg taaggggtct cctgcctgta aaggtgacct aacacgtcgg atgcttattt
     8521 ccatggatta aattctcctg tttcaatgag atccaaaact acgtcaacaa cgttggcatc
     8581 atattttatg cctctgcccc ggcttaattc ccttataact ttatccttgg tcctggcggc
     8641 tctgtagggc ctgagggtgg acatggcttc tgccacatcg caaacagtaa ttatacgtac
     8701 atttatgctg atctggcaac ccgaaagccc actggggtat ccactcccat ccagcttctc
     8761 atggtgattg agcacaatat cccttatatt ccacggcagt tcggcatcgc ttaatatttc
     8821 aaagccgatg cgcggatgtt ccctgatcag ggttaattcc gtatccgtca acggcggcat
     8881 cttatttagt atttgggtag gtatacctat ctttccgaca tcatgcaata acccgcctat
     8941 atacagttca tttatatcgt cctgtccaag tcccatccgt cgccccacta ttgcagccaa
     9001 tttggcaacc ctctcctgat gaccggcagt atacgcatcc ctaaacccca aggtacggca
     9061 tacggttttg gtcaggtcga agaaggcctt ctcaaggatt cttttggttc tctcattatc
     9121 ctcccttatc ttccgttcca tgccgtgctt gtaaagggca atttctatca ccatattgag
     9181 ttccctagga tttatgggct ttaggatata cccaaagggt tcggtaatct tggctctccc
     9241 cagggtatcg tcgtccgcat gggaagtgat atatacaagg ggtaaatcga attccttgtg
     9301 aacgacttgg gctacctcaa taccgtccaa actcccccct agttgaatgt ccattagaat
     9361 aaggtcagga ttttggaggg agattttatc cagagccttt tcccctgtgg attcgattcc
     9421 gactacccta tacccctgac ccatcagcat cttttgaaga agatttgccg ttattggctc
     9481 gtcttctacg ataaggatgt ttgccttttt tttcgcagat atctcagcct cgcttatgct
     9541 attatcccat aaatctctag tacgtccact tcgtattata ttcatcgtac cctccatgct
     9601 aatgtgctga tattgtttat gttacgggaa agattaattg ctacagatca cccatcttcc
     9661 aggtatattc taattctatc catctcgttg cgtaactcgt ccaatatccc catagcccct
     9721 tctgtcctgc cctcagaagc taaaccccca agctgggtga ggagttccac agcgtgatgg
     9781 gcaccgaaat ttgaaaccgc acccctcata ccatgggcac ttttctccaa cttcttcgga
     9841 tcacgttggc ttattgcgtt ggctatctct tcaaggtttt taggatagct gtctataagt
     9901 gtcctaataa gttccttaac gaatttttcg tcatgcccag tagtcagatt gagccctgac
     9961 agatcgacgg gaattttagg ttttgaaaac ctatccagaa tatcgtagag cacctccgga
    10021 tttaagggtt tggatacata cccatccatt ccggcatcaa tacacttctc cctgtcaccc
    10081 ttcatcgcat atgcggtcat agctatgatg ggtatatgat tgcccgtaat ctgttccatt
    10141 tccctaatcc tgcgggtggc ctccaatccg ttcatctccg gcatctgaac atccattaag
    10201 atcatatcaa attcaatacc gtcccttatg gtctctattg cctcgaggcc attagatacc
    10261 gatattgcgc tataacctct tttttcaagt agggcggtag ctagcttttg atttattata
    10321 ttatcttcgg cgattagcac cctacctagc ccatgccgtc tccccttttg atcttcccta
    10381 aaaacggcga ttgatccatc ggtctcttta tgggctgtat gtttatgatg tatagtttta
    10441 gatatagcat caagtagctc ctcttcccca ataggcttaa ttaggtacgc ctcgataccc
    10501 atttccttgc acttggtcat accatccttt atctccatgg aagtgagcat catgatggtg
    10561 gtgtccttta ggcctgtgtg attttttata tgttctgcca cctcaaatcc agtcatcccg
    10621 ggcatatgcc cgtccaataa aattatatca atgggtatat tatcttctaa atattcggtc
    10681 atcaattgga ttgctgtcac cccgtcacag gctgaggcta cataggcgcc ccttcgcttt
    10741 agtattttct ccaatattat acgattggcc ctattatcat cgactactaa aatccgaagg
    10801 cacgatatgt ctaccatact atcggggtca gcaatcatct gttcaccctt tgcaaaatag
    10861 gcggtaaagg taaagatact gccctcccct acccggcttt tgacatccat tcggccgccc
    10921 atcatttcca ccaactgttt ggctatggac aatccaagcc cagttccccc gtatttacga
    10981 gtatgggtat cttctaattg gctgaaactt tgaaaaagcc tgtccatctt atctcccggt
    11041 atacctattc ccgtgtccct tatttcaaag ctgagcataa cggatgtatc cagctcttta
    11101 accatattta tatccaggct aatctcccct gtatcggtaa attttaccga gttgcccaac
    11161 aagttataca aaacctgttg gagtctgacc ccatctccaa ctaggttcaa gggaacatcc
    11221 ggatcaatgt aatacatgag ttccaaattc ttttcatgtg ccctcagggc caaggtatca
    11281 cataccttac caactatttc attgagagca aagggggaat tatccatttc cagcctgccg
    11341 gcctctattt ttgaaaaatc cagaatatca tttatgattt gtagtaggga caacgccgat
    11401 tccttggcca tggtaatata ttcccattgt tcgttgttca gttctgtaga aagtgccagc
    11461 tcggtcatac ctaaaactcc attcatagga gtcctaatct catgactcat cctcgctaga
    11521 aattctcctt tagcccgatt ggctgtttcc gcctcgtcct tggcatctct aagctcatcc
    11581 atcattttac ttctctcggt aatatcccgg attacgctaa gaagcaacct tttgttatct
    11641 atagttacgc cctgggaact cacttccacc cgaaaagagc tgccgtcttt tcgggcatga
    11701 acgccttcaa aaagaatgcc tgtatccccg gccatcgcca tctggttctc caccagagca
    11761 ttttcatccc caccccttag cctaaatata ctcatggaaa ggagctcctc cctagaatat
    11821 ccgtaggcct ttaccgctgc ttcgtttgcc tctataatcc ttccgtccat ttgtataaac
    11881 attatacgga ttgatccttg cccagtatat ccccttcttt aatcccggtt aaatggccca
    11941 tggcgtgatt ccatgtcaaa acgcggtcgt ggttgtccac gacaaaggt
//
LOCUS       DASRNX010000034         8868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_590447,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000034 DASRNX010000000
VERSION     DASRNX010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590447"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFD89_01275"
     CDS             <1..395
                     /locus_tag="VFD89_01275"
                     /inference="COORDINATES: protein motif:HMM:NF023435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HZJ56857.1"
                     /translation="EGTHYDLNDDGYVIPKAANGWSDSHGGMWSLGNQFKQKLSASED
                     PLKYEQMQALTAESWAHESLGFRFKRDSVAAEFTSINAVITEWERSLNTGVRVDEYDD
                     FLKALEEAGIDKVLEEAKAQYAEWKANN"
     gene            638..2038
                     /locus_tag="VFD89_01280"
     CDS             638..2038
                     /locus_tag="VFD89_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZJ56858.1"
                     /translation="MSYNKSTKGNKNSAGVDMTVGNPTRLIIYFALPLLAGNILQQLY
                     NMVDSMVVGNFAARGTEALAAVGTTFPVIFMITSLFMGVGQGATIKISQYYGANDMGG
                     LKRAVDTIYIFLFAVSIPIALVGVLISRPMLRLMNVPDGILPMATTYMRIIFIGTLAS
                     FGYNINSGILQGLGDSRKPLLFLAISTVINIILDLVFVIVFHWDVAGVAWATILAQLF
                     SFLYSIHYINTRGGHFGISYKDLEFDGEILKESIRIGLPAGIQNLLFSLASMTLQGLI
                     NGYGEKFIAGFNGANKIDAFAFLPLVSFSVAITTYVGQNVGARQMDRVNRGVKSALLM
                     STIISILMAVVLLPSGNLLMRLFTQDPEVIHAGIEYLKRVLPFYTMLGAVFILNGALR
                     GAGEAFVPMVGSLISMWLARVPAAYILAHYFGGNNLYLSYPVGWFIGIIIVVVYYLKG
                     TWKEKAISRLEPKGTA"
     gene            2156..3160
                     /locus_tag="VFD89_01285"
     CDS             2156..3160
                     /locus_tag="VFD89_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZJ56859.1"
                     /translation="MVKIRAFLVFVMTMLFVAGLSGCYLFPKEEEVLAPPLIEPPKIT
                     YETTKAARGIIEKKITGSGTFVSVEQGNMFFKNRGGRLKKVYVKVGDTVEKGELIAEL
                     DSGDLENQIKQQKIHLKKAETRRNQVRTYSEDKYERTMAQYDVDLAKLSYENLQRELN
                     ESKLTADMSGHVVYVDYNVREGDYINAYQPLVRVADPDRLQLQISGSNTSDFKLGMEA
                     DVIIKKKTYPGTVVMTPADLPLDTPDNLKDIVRIDTPDLPDDVKMGDNAQISLILDRR
                     EDVIIVPRNLVRNYMGRKYVLVLEDDLKKERDVEIGLETPTETEIMKGLEEGELIIIR
                     "
     gene            3179..6043
                     /locus_tag="VFD89_01290"
     CDS             3179..6043
                     /locus_tag="VFD89_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZJ56860.1"
                     /translation="MLIMVLRKMINNKWMALCLLIGFILAVAMVSSIPIYTDGVLQRM
                     LTRDLENHQVTTGFFPGRYIASANLYSNYDEDSRLKALNLFDERMTDTFPKEIGLPVR
                     AYSRVLSAEYFSAMPKIQREEKPARRSVSLSTMHELEEHTQLINGRMFSGEKQDGIYE
                     AIVTEHAMQKLDLRLDEEYVLEDFVKRTKEPIKVRIVGIFTVKDVADPYWFQGLSAYN
                     MDFMIDEELFLNDFVNGDLSLATKGQWYYAFDYHKITLDNTPQIIDAHNSQYKWLKER
                     RVEFNMPAISVLEKYYEREKQLMTTLWVVQVPILMMLGFYLLMVSRLAIEQEQNEIAV
                     MKSRGASSLQVFLGYLMESLILGFIALLAGPPLGLFICGFLGSANGFMEFVQRTALPI
                     SLTMKAYLYSVGAVLISIITMLVPAFISSRTTIVEYKRKKTRHTGRSLWEKYFLDILL
                     LAVSGYGLYSYNLRQQTLEITGASGTELAIDPMLFIISTLFILGVGLVLLRIYPYLVR
                     LLFLAGKRIWSPVMYASFVQVGRSGGQDQSLMLFIILTLSIGIFNANAARTLNNNIEE
                     KIRYSIGADLNIMAHWESNEVPAGGPGMGGGPPESQVMESLNKEPVQYREPPFGPFTE
                     LSGADKVTKVFRQNDIAVQTSTRERTKGKTELLGVIPDEFGQTAWFRNDLLKTHWHNY
                     LNLLSMGDPRAALVTRSLNENYEVKKGDSLWISWGDQGYLEVVVYEFIDYWPTFNPIL
                     REGQKDTADLIVAQFDYIQANNALEPYEVWVKKAPGVSSQAIYDEILEKEIKIEKLTD
                     TSQEIIKTKNDPMLQGTNGALTLGFIVTMSVSTIGFLIYWILSIRQRVLQFGIFRAMG
                     LSVIKIIGLLAWEQLLISGTAILIGVVVGGLTSDLFVPLLQMVYSAADQVPPFKVAAS
                     GQDYIKIYTVVAVMLSAAIGFLGMLISKIKIHQAIKLGED"
     gene            6219..8018
                     /locus_tag="VFD89_01295"
     CDS             6219..8018
                     /locus_tag="VFD89_01295"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016717932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HZJ56861.1"
                     /translation="MKTLKPENRYQGTLPKIGIRPAIDGRRRGVRESLEEQTINMARA
                     AAKFLEENLRHANGLPVECVIADTCIGGVAEAARAADKFQREGVGVSITVTPCWCYGS
                     ETMDMDPSTPKAVWGFNGTERPGAVYLAAVLAAHNQKGIPAFGIYGRDVQDAGDTSIP
                     EDVKGELLKFARAGLAVASMKGKSYLSMGGTSMGIAGSMVNPDFFERYLGMRVESVDM
                     SEFIRRFDEGIYDEEEFERALAWTRENCTEGPDNNPIEVQVSRTKKDEVWETVVKMAL
                     IARDLMVGNEKLAELGFGEEALGHNAIAAGFQGQRQWTDHFPNGDFMEAILNSSFDWN
                     GIRQPYLVATENDSLNGVAMLFGNLLTNSAQVFADVRTYWSPESVKRVTGEELTGRAA
                     NGIIHLINSGSASLDGSGRQEIDGEPAMKPYWEITPKEAQDCLDATSWRYANLGYFRG
                     GGYSSQFLSRGNMPVTMTRINLIKGLGPVLQIAEGYTVDLPKEINDVLDKRTDPTWPT
                     TWFVPNLTAKGAFTDVYSVMNNWGANHGVLSYGHIGDLFITLASMLRIPVAMHNVSED
                     RIYRPSTWGAFGTNDPEGADYRACENFGPLYGK"
     gene            8124..8291
                     /locus_tag="VFD89_01300"
     CDS             8124..8291
                     /locus_tag="VFD89_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56862.1"
                     /translation="MSFFKGADDSLLFFFLLLVIIFCNCGLFRDGSELLFFFLLLVVL
                     FMGEDICGCRR"
     gene            8619..>8868
                     /locus_tag="VFD89_01305"
     CDS             8619..>8868
                     /locus_tag="VFD89_01305"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HZJ56863.1"
                     /translation="MKVLLLTVTTGHGHTQAAKVIMECLEKNGAQCRLLDTLEYINPI
                     LGESVDKGYLYSTRYTPHMYGRIYDAIDNRDMGDGKFSI"
BASE COUNT         2451 a         1835 c         2306 g         2276 t
ORIGIN      
        1 ttgaaggcac acattatgac ctgaacgatg atggctatgt aattccaaaa gctgccaacg
       61 gctggtccga ctcccatggc ggtatgtggt cattgggtaa ccaattcaag caaaagctat
      121 ctgcttccga ggatccgttg aagtatgagc agatgcaggc tttgacagct gaatcttggg
      181 cccatgaatc cctaggcttc cgctttaagc gtgacagcgt agctgcagag tttacctcta
      241 ttaacgcagt tatcaccgaa tgggaacgtt ccttgaacac cggcgttcgc gtcgacgaat
      301 atgatgactt cttaaaggct cttgaagaag caggaatcga caaggtccta gaagaagcaa
      361 aagctcaata tgccgaatgg aaagcaaata actaagcata taagctgata acagagcact
      421 aaaaaggccc ccgctaaggc aggggtcttt tttatgcgtt ggagccggat cgtgactcat
      481 agatgggata taaacttata tgctcccaaa acttgttatt ccatattcat tcctttataa
      541 tgtatgatag ccttatgtag gaaaggttca tcggtctatt ctgggaggtg atcaggggat
      601 attttctata tatacacaaa tgcgaaggag ttaggatatg tcatacaata aaagcacaaa
      661 gggtaacaag aattcagccg gggtggatat gaccgttggt aaccctactc ggctgattat
      721 ttattttgcc ctgccccttc tggcaggcaa tatacttcag caattataca atatggtaga
      781 tagcatggtg gtgggaaatt tcgccgccag gggaacggag gccctagcgg ccgtggggac
      841 tacctttccc gtcatattta tgatcacttc attgtttatg ggggttggtc aaggggccac
      901 cataaagata tcacaatatt atggggctaa cgatatgggc ggtctaaagc gcgccgtaga
      961 caccatatat atatttttat ttgcggtatc cattcccatc gcgctggtgg gtgtacttat
     1021 aagccggccc atgctcaggc tgatgaacgt ccccgacgga attttgccta tggcaacaac
     1081 ctatatgaga ataatcttta tagggaccct ggccagcttt ggatataata ttaacagcgg
     1141 gatccttcag ggattggggg atagcagaaa acccctactg ttcctggcta tatccaccgt
     1201 tatcaatatt attctggatc tggtattcgt aatagtcttt cactgggatg ttgcaggagt
     1261 ggcctgggct accatcctgg cgcagctatt ttcattttta tacagcatcc actatatcaa
     1321 caccagaggg ggacattttg ggatatccta taaagacctg gagttcgacg gtgaaatcct
     1381 aaaagagtcc atccggattg gtcttcccgc agggattcag aatttattat tctccctagc
     1441 gtccatgacc ctgcaagggc ttataaacgg ttacggcgaa aaatttattg ccggatttaa
     1501 cggggccaac aagatcgatg cctttgcatt tcttcctcta gtgagctttt ctgtggccat
     1561 aaccacctat gtaggacaaa atgttggagc aaggcaaatg gacagggtca acaggggggt
     1621 aaaatcggca cttttaatgt ccaccatcat atccatattg atggcggtcg ttctgctgcc
     1681 gtctgggaat ttgctcatgc gattgtttac ccaggatccc gaggtaatcc atgccggaat
     1741 cgaatattta aagcgcgtcc taccctttta taccatgttg ggtgcagtat ttatcctaaa
     1801 cggggcattg aggggtgccg gtgaggcctt tgttcctatg gtaggttcgc tgatctcaat
     1861 gtggttggcc agggtgcctg ccgcctatat tcttgcacat tattttggcg gcaataacct
     1921 gtatctcagc tatcccgtag gctggttcat agggataata atagtggtag tctactatct
     1981 gaagggaaca tggaaggaaa aagccatatc acggctggaa ccaaaaggta cggcatgacg
     2041 ataacggctc aagggatgac tttcattatc ctttgctttg tagtataatc gaatttatag
     2101 gcatttaatc catacagaaa caatcttaat tacttttaaa catgggggcg gtaatgtggt
     2161 aaagattagg gcatttctag tgtttgttat gacgatgtta tttgttgctg gcctttccgg
     2221 gtgttatcta ttcccgaagg aagaggaggt actggcccca ccgctgattg aaccacctaa
     2281 aattacgtac gagactacaa aggctgctag gggaattatt gagaagaaga taacaggcag
     2341 cggcactttt gtatccgtcg agcagggaaa tatgttcttt aagaacaggg gcgggcgttt
     2401 aaagaaggtc tatgtaaagg tcggggatac tgtagaaaaa ggggaattga tagcagaact
     2461 ggatagcggt gatctggaaa accaaatcaa acaacaaaaa attcatctca agaaagccga
     2521 gaccagaagg aatcaagtca ggacctattc cgaggataaa tatgagcgga ctatggccca
     2581 atatgacgtg gatctggcaa agctctccta cgaaaactta cagagggaat tgaacgaaag
     2641 caaattgacc gcggacatga gcgggcatgt agtatatgtg gattataacg taagagaagg
     2701 cgattatata aatgcatatc aacccttggt ccgcgtagct gatcctgaca ggctccagct
     2761 tcagataagc gggtctaaca cttcggattt taagctgggt atggaagcgg atgttataat
     2821 aaagaagaag acatatccgg ggacggtagt gatgacccct gccgatctgc cccttgatac
     2881 gccggataat ttgaaggata ttgtacgcat cgacacgccg gatttgcctg acgatgttaa
     2941 gatgggcgat aatgcgcaga ttagccttat attggacagg cgtgaggatg tcatcatcgt
     3001 cccaagaaac ctggtcagaa actatatggg acgaaaatat gtcctggtat tggaagatga
     3061 ccttaaaaag gaacgcgatg tagagatagg gctggaaacc cctacggaga ccgaaataat
     3121 gaagggctta gaagaaggag aactaattat tatcagataa tagaggggga tcttgaccat
     3181 gctcattatg gtattgagga agatgataaa caacaaatgg atggccctct gcctgcttat
     3241 cggtttcatt ttggctgtgg ccatggttag cagtattccc atatataccg acggggttct
     3301 gcagcggatg ttgaccaggg acttggagaa ccatcaggta accacgggat ttttcccggg
     3361 gcgctacatt gcctcggcga atctttattc caattacgat gaggattcca ggcttaaggc
     3421 ccttaacctt tttgacgaaa ggatgaccga taccttccct aaggagatcg gacttcctgt
     3481 gagggcatac tctcgtgtcc tttctgcgga atactttagc gctatgccca aaatccaaag
     3541 ggaagagaaa ccggcaaggc gaagcgtcag tttaagcaca atgcacgaac tggaagagca
     3601 tacacagctg ataaacggac ggatgttttc cggagaaaaa caggacggca tctatgaagc
     3661 catagttacg gaacatgcca tgcagaagtt ggacctgaga ttggacgaag aatatgtgct
     3721 ggaggatttc gtaaagcgaa caaaagagcc tataaaggtc aggatcgtag gaatatttac
     3781 cgtaaaggat gtggctgacc cctattggtt tcaggggtta tcggcatata atatggattt
     3841 tatgatcgac gaggaactct ttctaaatga ctttgttaac ggtgatttgt ccctggctac
     3901 caagggtcag tggtactatg cttttgatta tcacaagata accctggata acacccctca
     3961 gattatagat gcccataata gtcagtacaa atggcttaag gagcgcaggg tggaatttaa
     4021 tatgccggcg atttcggtgc tggagaagta ttatgagagg gaaaaacagc ttatgactac
     4081 cctatgggtg gtccaagttc ccatccttat gatgctgggg ttttatcttt tgatggtatc
     4141 gcgccttgct atagagcagg aacaaaacga gatagcggtt atgaagagca ggggggccag
     4201 cagccttcaa gtctttttag gctacttaat ggagagcctt atactagggt ttattgctct
     4261 cttggcagga ccgcccctag gcctgttcat atgcgggttt ttaggatctg ccaacggatt
     4321 tatggaattt gtacaaagga cggcacttcc catatccctt actatgaagg cctacctgta
     4381 ttccgttggg gccgtgctga tttccattat tacgatgctg gttccggcct ttatatcatc
     4441 caggacaacc atcgtcgaat acaagagaaa gaagaccaga catacgggca gatccctatg
     4501 ggaaaaatac tttttggata tattgctcct ggccgtatcg gggtatgggc tctacagcta
     4561 taatttaaga cagcagaccc ttgagattac cggcgccagc ggtaccgaac tggccatcga
     4621 tcccatgctc ttcatcatat ccaccctgtt tatactgggt gtggggctcg ttttgcttag
     4681 gatatatccg tacttggttc gtctgttatt ccttgccggt aagaggattt ggtcgccggt
     4741 tatgtacgct tcctttgtac aggtgggaag atccggagga caggaccaat cgctgatgct
     4801 gtttattatt ctcaccctat ccataggaat attcaacgcc aacgctgccc ggacattaaa
     4861 caacaatata gaggaaaaga ttagatattc aatcggtgca gatctcaata tcatggccca
     4921 ttgggagagc aatgaagtgc ccgccggggg ccccggcatg ggcggcggtc cgccggaaag
     4981 tcaggttatg gagagcctta acaaagagcc cgttcagtat agggagcctc ccttcggccc
     5041 atttaccgag ctgtcgggtg ccgataaggt taccaaagtt tttaggcaaa atgatatagc
     5101 ggttcagacc tcgactcggg agaggacaaa ggggaagacg gaactcctcg gggttatccc
     5161 cgatgaattc gggcagactg cctggtttag aaacgatctg ttgaagaccc attggcataa
     5221 ttacctaaat ctactttcca tgggcgaccc cagggctgca ctggttacca gaagcctaaa
     5281 tgagaactat gaagtaaaga aaggtgattc cctatggatc agctgggggg accagggata
     5341 tttagaggtt gtagtctatg agtttataga ttattggccg acctttaatc ctatcctcag
     5401 ggaaggacag aaggataccg cggatctcat agtggcccaa ttcgattata tccaagctaa
     5461 taatgctctg gaaccatatg aggtatgggt caaaaaagct cctggcgttt ccagccaggc
     5521 gatctacgat gagatactgg aaaaggaaat taagatagaa aaactgactg acaccagcca
     5581 ggagatcata aagaccaaaa acgatccaat gcttcaggga actaacgggg cattgacctt
     5641 ggggttcatc gtaaccatgt ctgtaagcac catcggtttt ttaatctact ggatattatc
     5701 cataaggcaa agggtactcc aatttggaat attcagggcc atgggacttt ccgtaataaa
     5761 gataatagga ttattggcct gggagcagct ccttatatct gggaccgcca tcttaatagg
     5821 cgtggtggta gggggactga ccagtgatct cttcgtgccg cttttacaga tggtctacag
     5881 tgcggcggat caggtgcctc ccttcaaggt ggctgcgtcg gggcaggatt atattaaaat
     5941 ctataccgtt gtcgcagtaa tgctgtcagc ggctatcggg ttcttgggca tgctcatatc
     6001 caagataaag atacatcagg ctataaagct gggtgaggac tagactttgt atgaaacaat
     6061 tgcattagca attgtaacac acaggtttca acggggtgaa cagatatata ccatacgctt
     6121 actacttgcc ttaattagtg atttcgttta tggaggggcg aatttatcac cccgttataa
     6181 taaataaagg gggacgaatt tatatggctt ctaacgagat gaagacttta aaaccggaga
     6241 acagatacca gggtactctg cccaagatag gcataagacc tgccattgac ggacgccggc
     6301 gcggggttag agaatcccta gaagagcaga ccatcaatat ggcaagagca gcggccaagt
     6361 tcctagaaga gaatttacgc catgccaacg gcttgcccgt ggaatgtgtc atcgcggata
     6421 cctgtatagg cggggtagcg gaagccgcca gggcagccga taagtttcaa agggagggag
     6481 tgggggtctc cataaccgtg accccttgtt ggtgctacgg atccgagacc atggacatgg
     6541 atccaagcac acccaaggcc gtatggggat ttaacgggac cgaaaggccg ggcgctgtct
     6601 atttagccgc cgtactggcc gcccataacc aaaagggaat acccgctttt ggtatctacg
     6661 gcagggacgt tcaggatgcg ggggatacat ctattcctga ggatgtgaag ggggaactcc
     6721 taaagtttgc cagagctgga ttggccgttg cttctatgaa gggaaaatcc tacctgtcca
     6781 tgggcggcac ttccatggga atcgccggat ccatggtgaa tccggatttt tttgaaagat
     6841 acttgggcat gcgggtggag tccgtggaca tgtcggaatt tataagacgc tttgatgagg
     6901 ggatctacga cgaggaagaa tttgaaaggg cccttgcctg gacccgggaa aactgcacag
     6961 aggggccgga taacaaccct atagaggttc aagtatccag gaccaaaaag gatgaggtat
     7021 gggaaacggt ggtgaagatg gccctcatag ccagggatct aatggtgggc aacgaaaagt
     7081 tagcagagct gggatttggc gaagaagcgc tgggacacaa tgctatcgca gccggattcc
     7141 agggccagag gcaatggacg gatcacttcc caaacggcga ctttatggag gctatcctaa
     7201 actcctcctt cgattggaac gggattcgac agccatatct cgtagcaaca gagaacgaca
     7261 gtctaaacgg ggtagcgatg ctctttggaa atctcttgac caattcagcc caggtctttg
     7321 ccgatgtaag gacctattgg agtccggagt ccgtaaaaag ggtaacggga gaagagctga
     7381 caggcagagc cgctaacggg ataatccacc ttataaattc gggttcggca tccttagacg
     7441 gttccggccg ccaggagatt gacggagaac ccgctatgaa gccctactgg gaaataaccc
     7501 ccaaggaggc acaggattgt ctcgacgcta catcatggag atatgcaaac cttggatatt
     7561 tccggggtgg cggatattcc agccaatttt tatccagggg aaatatgcct gtaaccatga
     7621 caagaataaa tctaatcaag ggtttgggac cggtacttca gatagctgaa ggatataccg
     7681 tggatttacc taaggagata aacgatgttc tggataaaag gaccgatccc acctggccga
     7741 ccacctggtt tgttccgaac ctgacggcaa agggggcatt caccgatgtc tattcggtta
     7801 tgaataattg gggcgccaat cacggggtcc tgtcctatgg ccatatcggt gatctgttca
     7861 ttaccttagc gtccatgctc aggattccag tggccatgca taatgtatcg gaggaccgta
     7921 tttataggcc cagcacatgg ggagccttcg gtaccaacga tccggaaggc gctgattaca
     7981 gggcctgcga aaactttggt cccttgtacg gaaaatgatt agggagggcg ttcccattca
     8041 cctttatagg gccggggcat ataatggtag tgaaatacca tagaagtgcc ttggcacagg
     8101 gaaaactata agggggtatg ggtatgtctt tctttaaagg cgctgacgat agcttattat
     8161 tcttcttcct tctcttggta attatctttt gcaactgtgg tctttttagg gacggaagcg
     8221 aactattgtt tttcttcctg ctcctggtcg tactctttat gggagaagat atatgcgggt
     8281 gccgcagata gtagtttcaa ataaaaagcc tcctcttttt aatggggagg ctttttattg
     8341 cctattttgg cccgttggta tagaatgtag gtagctatgg gacagtgaca ttagatcccg
     8401 taaacttgga tagttaaata attaaccggc caatctgggg aataaggact ttaattatca
     8461 agggtgatgc gggaatttcc gtagtatatt ttgtaatttc accccattaa ttcaaatcat
     8521 ctatgggaaa ttggcttgta gaagcaaaaa cattctctta atattttcgg atagggatta
     8581 tattgttaca gaaaccgtac tgttagggag gtatcaaatt gaaagtttta ttattgactg
     8641 taacaaccgg tcatgggcat acacaggcgg caaaagtaat tatggagtgc ctggaaaaaa
     8701 acggggctca gtgtagactc ctggatacct tagagtacat aaacccgata ttgggtgaat
     8761 cggtggataa aggctatctg tattccacca ggtatacccc tcatatgtat ggaaggatat
     8821 acgatgccat agataacagg gacatgggcg acggaaagtt ttctatat
//
LOCUS       DASRNX010000035         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_643910,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000035 DASRNX010000000
VERSION     DASRNX010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643910"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..648)
                     /locus_tag="VFD89_01310"
     CDS             complement(49..648)
                     /locus_tag="VFD89_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56864.1"
                     /translation="MNNIGNIGYINGSGNTAAAKHLFDLAKNRKAPFRSTASSLGYHR
                     DINVRGQGSINRATSVQWYYNLNLKTPTNSNTASGVIDLLKMENIRKVNNLKELELDW
                     NGNGGLPFSNDSIFFFRDIIEHIIKQPNIAPTGRESLLMQYELEDRSFLGFEVFKGKV
                     DKVLIPQRDYNRAVEEEITNDYIVQINKSVESFYGYELH"
     gene            complement(641..1066)
                     /locus_tag="VFD89_01315"
     CDS             complement(641..1066)
                     /locus_tag="VFD89_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56865.1"
                     /translation="MSYVSTFIYCLNTTKEQSEAGESINALGVLTALTPDFIPGSFSF
                     SVIFSVMDIDDRDSHEIKLVFSNCAGNELVNTGVINIPKIEDKNAIDLPKEVSGLIMS
                     LDLRNVTFLKEGYYCTTVWFDGDKLGEYKIYVKGKIRNE"
     gene            complement(1453..>2888)
                     /gene="ftsH"
                     /locus_tag="VFD89_01320"
     CDS             complement(1453..>2888)
                     /gene="ftsH"
                     /locus_tag="VFD89_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZJ56866.1"
                     /translation="FMQQAQGGGGGNRVMSFGKSRAKVHTDEKRRTTFRDVAGADEEK
                     EELKEIVDFLKNPKKYIELGARIPKGVLLVGPPGTGKTLLARAVAGEAGVPFFTISGS
                     DFVEMFVGVGASRVRDLFEQAKKNSPCIVFIDEIDAVGRHRGAGLGGGHDEREQTLNQ
                     LLVEMDGFSDNEGIIILAATNRPDILDPALLRPGRFDRRVLVGVPDVKGREEIIQVHS
                     RGKPLDPEVDIKVLAKRTPGFTGADIENMMNESAILVARRSGKAIEMRDLEEAITRVI
                     AGPEKRSRVITDKDKRLVAYHEAGHAVVAKLLPTADPVHEISIIPRGMAGGYTLTLPE
                     QDKYYVSKSEMLDDITQLLGGRVAEALVLNDISTGASNDIERASNMARKMVTEYGMSE
                     NLGPITYGGKHEEVFLGRDFSTHRNYSENIASAIDNEVRSIIQTAYSKAEQLLNGHMD
                     GLHRVAQALLDREKLDMQEFEELFAGA"
BASE COUNT          729 a          757 c          529 g          873 t
ORIGIN      
        1 gtatctgggt atgatcttcg attgaatcta tataattgtt catcatcatt aatgtaattc
       61 atatccataa aagctctcca cagatttatt gatttgaact atataatcat tcgttatttc
      121 ttcttctaca gccctgttgt aatccctttg ggggattaaa accttgtcta cctttccttt
      181 aaacacttca aatccaagaa agcttctatc ttccaattca tactgcatta acaaactttc
      241 ccttccagtc ggggctatat ttggttgttt gataatatgc tctattatat ctctaaaaaa
      301 gaaaatagaa tcatttgaaa atggcaaccc gccattcccg ttccaatcta attccaattc
      361 tttaagatta ttgaccttcc ttatattttc catctttaac aaatcaatca ctccagacgc
      421 ggtgttgcta ttagttggag tcttcaaatt taagttataa taccattgta cgctggttgc
      481 ccgattaatg ctaccttgtc ccctgacatt gatatccctg tgataaccca gagatgatgc
      541 agtagatcta aaaggggctt tcctattttt agcaagatca aataaatgtt ttgctgccgc
      601 tgtgtttccc gaaccattaa tatatccaat atttccaata ttattcattt cttatcttcc
      661 ctttgacgta tatcttatat tcacccaact tatccccgtc aaaccatacg gtcgtgcaat
      721 aatacccttc ttttaaaaag gtaacatttc gaagatccag gctcataatc aatccgctta
      781 cttccttggg aagatcaatg gcgttcttat cttcaatttt tggaatgttg atgactcctg
      841 tatttacaag ctcatttccc gcacaatttg aaaaaactag ttttatttca tgtgaatccc
      901 tatcatcaat atccatgaca gaaaaaatta cagaaaaaga aaacgatccg gggataaagt
      961 cgggcgtcaa tgcggttagt acacctaacg catttatgga ttcccccgcc tcactttgtt
     1021 cttttgtcgt atttaggcaa tatataaacg tggatacata agacatacat caatcccccc
     1081 atagattttt taccgtttgg taacccgcac tataaaataa tctgatatgc tggacttaag
     1141 cggaaattgg caaagtttcc cccataacaa ctatatagca taattttact ttagtaaacc
     1201 cacaatttat tgtatatcct gttagttgaa tcaccatata tagtaattga gcaaagcatt
     1261 tagccccaat tgaagttctg gattttatac caaaagtcaa aatagcaaaa agggaggtct
     1321 cggatttatg cctccaacct cccatatctg acctttaaaa ggatcagctt tgctgtccct
     1381 taccgcctat taattccgat tatgaatgaa aatcttatat cctaggcgtc cgaactaacg
     1441 ggctaatgat ttctatgccc cggcgaaaag ctcctcgaac tcctgcatat ccagcttctc
     1501 cctgtcaagc agggcctggg ccactctatg aagcccatcc atatgtccgt ttaacagctg
     1561 ttccgccttg gaatacgcgg tctggattat acttctgacc tcgttgtcta tggccgatgc
     1621 tatgttttca ctgtagtttc tatgggtgct gaaatccctt cctaaaaaga cctcttcatg
     1681 cttgccccca taggttatgg gacccaagtt ttcgctcata ccgtactcgg taaccatctt
     1741 cctagccata ttgctggccc tttcgatgtc gttggacgct cccgtgctta tgtcgtttag
     1801 gacaagagcc tcggcgaccc tgcccccaag gagctgggta atatcgtcca gcatctccga
     1861 tttggaaaca tagtacttat cctgttcggg aagggttaag gtataacccc ctgccatccc
     1921 cctggggata atggatatct catggaccgg gtctgcggta ggcaacagct ttgccaccac
     1981 agcatggcct gcctcgtgat aggccaccag cctcttatcc ttgtcggtta taactcggct
     2041 ccgcttttcg ggacctgcta taaccctggt aatggcctcc tcaagatctc gcatttcgat
     2101 ggccttaccg gatcttctgg ccaccagtat ggccgattcg ttcatcatat tctctatatc
     2161 cgcaccggta aatccggggg ttctcttggc aagtaccttt atatcgactt ccggatccag
     2221 gggctttccg cgggaatgga cctgaattat ctcttccctg ccctttacat cgggcacccc
     2281 aaccaatacc cttctgtcga atctgcccgg tcttaagagc gccggatcca atatatccgg
     2341 cctgttggta gcagccagga taatgatccc ttcattatcg gagaaaccgt ccatctcaac
     2401 taagagctgg tttagggtct gctccctttc atcgtgtccg ccgccaaggc ctgcaccccg
     2461 gtgtctgccc acggcatcga tttcgtctat gaagactata caaggtgaat ttttcttggc
     2521 ctgttcgaat agatcccgaa ccctagaagc accgacacct acgaacatct ccacgaagtc
     2581 cgaaccgctg atggtgaaaa agggcactcc cgcctcgcct gcaacggctc tggccaacag
     2641 agttttaccc gtaccgggcg gacctaccag caatacgccc tttggtatac gagcgcccag
     2701 ctctatatat tttttaggat tcttaagaaa atccacgatc tccttgagct cttccttctc
     2761 ctcgtcagcc cccgcaacat cgcggaaggt cgtccgtctc ttctcatccg tatggacctt
     2821 tgcccggctt ttaccgaagg acatgacgcg gttgcctccc ccgccgcctt gggcctgctg
     2881 cataaaga
//
LOCUS       DASRNX010000036         3970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_113068,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000036 DASRNX010000000
VERSION     DASRNX010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113068"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1315
                     /locus_tag="VFD89_01325"
     CDS             92..1315
                     /locus_tag="VFD89_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ56867.1"
                     /translation="MSIKPLKIGLIGSGAISYTYLNTLTSGFSAVEVVGCSDLIPERS
                     KARAELFGIRQMTTEEILADPEIELVLNTTQLWNHHHVTKAALEAGKHVYSEKAMDHT
                     LEGAKENYELAKKKGLLVGAAPDCYMGSAWQTARKLLDDGYIGQPLVCYSMCMRGYSS
                     HERGPMPEGFVRDPMMGARGTTITYDMGGYYINVLLNLFGAVKRVSGYSQVFTGDREY
                     YNTKNPNYGKKIPAEGGENLMLGALEFENGVYGSLVLCSESFNPEVPRVDIYGTEGHM
                     SLPDPNYFGGWGNDVIILQRKGNGSESFKIPFTHGFSDTDPSVMPKSGKGEPCHNSWR
                     GVAVVDMAYAIRRGRPARSSAELALHTVEIIDAIDRSNEDDRVHTMASRPERPAPLKP
                     GYYGNVNVMEASLDS"
     gene            1562..2644
                     /locus_tag="VFD89_01330"
     CDS             1562..2644
                     /locus_tag="VFD89_01330"
                     /inference="COORDINATES: protein motif:HMM:NF044836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HZJ56868.1"
                     /translation="MARKVWEVPLELVHNKIPRYPGGREIDRFRGLKDCQDDGKPEAW
                     VGSTTRAAGIHKDRDLGLSEVILPSEDVILLKDLIDKYPKQLLGEKHFKRYGQELCIL
                     VKLLDAKNQLGLQTHPTREYAKAHFDSDFGKTESWYIIGLREDQEERPYVLLGFKEGV
                     TREIFEGLYDNEDIKSMEEWCHKIHVSPGDMFLVDAGVPHAVGPGCFLVEVQESSDIT
                     VGARKREFPTEAEELAYKNRLLGCYIYDGSSYGENLEKYKIPPVINRTDQGGTEKVLL
                     GRTNTSYFGITESEVRGEFPMENTGTFSIAIVLEGEGEIVYSGGTMDIRKGSEVFLPA
                     GIQDAILKSENNTTLKVVQAFPPEII"
     gene            2886..>3970
                     /locus_tag="VFD89_01335"
     CDS             2886..>3970
                     /locus_tag="VFD89_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ56869.1"
                     /translation="MARVKPVKVGLVGSGAISKSYLSSMIKNFSILDVVGCSDIIPER
                     SQNRAEEFEIEQLTNEEIFNNPEIEIVLNTTYPLSHYEVSKAALLAGKNVHSEKMMAV
                     TMDEARELVSIAKERGLRIGMAPDTFLGGGLQTARKLLDAGFIGEPLTARAKVMRGYH
                     MRGETEGDRLPFIMTPGGGIPYDMGGYYLHALIFLLGGINRVTGFAKTRKDNYVYENP
                     RHPKYKESFDYDTPNILVGALEFERGCYGTLTTISEAFGEIPKVEIYGTEGVLICGDP
                     NTFGGPIYLKHDNGSWKDFPPHEIPLTHGYNDGDYRGIGVADMAWAIRNNRPHRCSAE
                     LGFHTFEVINGIMESCNTGKVYEMTSK"
BASE COUNT         1162 a          821 c         1033 g          954 t
ORIGIN      
        1 gatgtccgat aacgatgcta tcgcaaagat cgtctccgaa agcacagatt ctgtatggcc
       61 taaataatat aacgagagga atgatagtct tatgagtatt aaaccactta agattggatt
      121 gattggaagc ggtgcaatca gttataccta tctaaacacc ttaaccagcg gattttcagc
      181 cgtagaagtt gtcgggtgct ccgacctcat ccccgaaagg agcaaggcac gtgccgaact
      241 gttcggtatc cgccagatga ctacggaaga aattctagcc gatcctgaga ttgagcttgt
      301 gctcaacact acccagttat ggaaccacca ccatgtaacc aaagcggctc ttgaggccgg
      361 gaagcatgtc tactctgaaa aggccatgga ccatacctta gagggcgcaa aagagaacta
      421 tgaactggca aagaaaaagg gtctgttggt cggcgctgcc cctgattgct atatgggttc
      481 tgcctggcag accgccagaa agctattaga tgatggatat atcggccaac ctctggtctg
      541 ctattccatg tgtatgagag gttatagttc ccacgaacgg ggcccaatgc ctgaaggttt
      601 tgtcagggat ccgatgatgg gcgcccgcgg cactaccatc acctatgata tgggcggata
      661 ctatatcaat gtgcttctaa acctatttgg tgcggtaaag cgggtcagcg gctattctca
      721 agtattcacc ggtgatagag aatattataa caccaagaat cctaactatg ggaaaaagat
      781 cccggcagaa ggcggagaga acctcatgct tggtgccttg gaatttgaga atggcgttta
      841 cggttcgtta gtgctctgct ccgaatcctt caaccccgaa gtccccaggg tcgatatcta
      901 cggtaccgaa ggccatatgt ccctgccgga tcctaactac ttcggcggct ggggtaatga
      961 tgtaatcata cttcagagaa agggtaacgg aagcgagagc tttaagattc ccttcaccca
     1021 tggattcagt gataccgatc caagcgttat gccgaagagc ggcaaaggtg agccctgcca
     1081 caacagctgg cgtggggttg ccgtcgtaga catggcatat gccatccgca ggggtcgtcc
     1141 ggcccgaagc agtgccgagc tggcattgca tacagttgaa atcatcgacg ctatcgatag
     1201 aagcaatgag gacgatagag tgcataccat ggcttcccgt ccggagagac cggcaccctt
     1261 aaagcccggt tattacggaa acgtcaacgt catggaggcg tctttggata gctaatagct
     1321 gaaaaaagat atagataggc tgtattcatt atatatttat tgttttaact gtggattagg
     1381 ggagagtcta cgtttgggat aggatacacc gaaggctctc ccgctaatct caattggatg
     1441 caggaaggat tgatactaca taaatgttgt gatataatgt agtgtaagag aaatgcggtc
     1501 ttgcgggaaa aaacaactat actatataag cttgcttcag cattttattt ggagggttgt
     1561 tatggctagg aaggtttggg aagtcccatt ggaattggtg cataacaaga taccaagata
     1621 tccgggcggt agggagatcg ataggtttag gggattaaaa gattgccagg atgatggtaa
     1681 gccggaagca tgggtaggct cgacaacacg tgcagctggg atacataagg acagggacct
     1741 gggtctatca gaggtaattt tgccatcgga ggatgttatc ctgttaaagg acctgataga
     1801 caaatacccc aagcagctat taggggaaaa acactttaag cgatatggac aggaactatg
     1861 catcttagta aaattattgg atgctaaaaa tcaactggga ttacagaccc atcccaccag
     1921 agagtacgcc aaggcccatt ttgattcaga ctttggcaag accgaaagtt ggtatattat
     1981 tggactgagg gaagaccagg aggagaggcc ctatgtcctg ctgggattta aagaaggggt
     2041 caccagggaa atattcgaag gattatacga taatgaggac atcaaatcca tggaagaatg
     2101 gtgtcacaaa atccatgtgt cgccaggtga tatgtttctg gtagatgcag gggtccccca
     2161 tgcagtcggt ccgggatgct ttttagtcga ggtccaagag tcttcagata tcaccgtcgg
     2221 cgcaaggaaa agggaatttc ccactgaggc cgaagaactg gcatataaga ataggctcct
     2281 gggctgttac atatatgacg ggagcagcta cggggaaaac cttgaaaaat ataagatccc
     2341 accagtgata aacagaacgg atcaaggcgg taccgaaaag gtattgcttg gccgaaccaa
     2401 tacgtcgtac tttgggataa ccgaaagtga ggttaggggt gaattcccca tggaaaacac
     2461 cggaaccttt tcaatagcta tagtcttgga aggggaaggc gaaatagtct acagtggggg
     2521 caccatggat attcgaaagg gtagcgaagt gtttttgcct gcgggtatcc aggatgcgat
     2581 actaaagagt gagaacaaca ctacgctaaa ggtggtacaa gcctttccgc cggagatcat
     2641 ctagggaata taaattattt ctatttttca gctgatgaaa tacataaagg ttgataaaat
     2701 acacaagggc tgttggatgt tcatttccat gtccatttgg agaaaaccaa ggcttattct
     2761 attaaaaaca gccatcgtct ccaatcgagg cataaaatct actttctgtt accataggac
     2821 taaaacgaga tttaattgta aaacctataa atacataaat tatttataag gagaggaata
     2881 tcatcatggc aagagtaaaa ccggttaaag taggcctcgt aggttccggg gctatcagca
     2941 aatcatatct tagtagcatg ataaaaaatt tctccatttt agatgtcgta ggttgttccg
     3001 atatcattcc ggagcgcagc caaaaccgcg cagaagaatt tgaaatagaa cagcttacaa
     3061 atgaagagat atttaataac ccagaaatcg agatagtgtt aaacaccaca tatccccttt
     3121 cccactacga agtatccaag gccgctcttt tggcggggaa aaatgtccac agtgaaaaga
     3181 tgatggcggt taccatggac gaagccaggg agcttgtctc catagccaaa gagagaggcc
     3241 tgaggatagg catggcacct gatacatttt tgggcggagg tctgcagaca gcccgtaagc
     3301 ttctcgatgc cggatttata ggtgaaccct taacggccag ggccaaggtt atgcgcgggt
     3361 atcatatgcg tggcgaaacc gagggggatc gcctgccctt tattatgacc ccgggcggcg
     3421 ggatacccta cgatatggga ggttattatc tccatgccct gatctttttg ctcggtggaa
     3481 taaacagggt taccggcttt gcaaagaccc gcaaggataa ctacgtatat gaaaacccga
     3541 ggcaccctaa gtacaaggag agctttgact acgatacacc aaatatactg gtaggcgctc
     3601 ttgaatttga gcggggttgt tatgggaccc taaccactat ttccgaggca tttggcgaga
     3661 ttcccaaagt tgaaatttat gggaccgagg gcgttctcat atgcggggat cccaatacct
     3721 ttggcgggcc tatatatcta aaacatgaca acggctcgtg gaaggatttc ccgccgcatg
     3781 aaatacccct tactcatgga tacaatgacg gggattatcg gggtatcggc gtagcggata
     3841 tggcatgggc tattcgaaat aacaggcccc atcgatgtag cgcagagctg ggcttccata
     3901 cctttgaagt aatcaacggt ataatggaga gctgtaacac agggaaggtt tatgagatga
     3961 catccaagtg
//
LOCUS       DASRNX010000037         3985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_485947,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000037 DASRNX010000000
VERSION     DASRNX010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485947"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VFD89_01340"
     CDS             complement(<1..726)
                     /locus_tag="VFD89_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZJ56870.1"
                     /translation="MILIKNGKVLTMNEKVYEKGDILIKGKVILEIGEDIEVPEGAEV
                     IDAGGMWVMPGIVDAHCHIGMWEDGIGFEGSDGNEATDPATPELRAIDGINPEDNCFR
                     EARENGITSVVTGPGSANVIGGQFAALKTYGRRVDDMVFKEPLALKVAFGENPKWVYD
                     GQKKAPSTRMATAAILRENLVKAQDYREKLEKGKADPDKMPDRDLKMEILVKVLNKEI
                     PLKAHAHRADDILTALRIRNEFDL"
     gene            complement(786..2939)
                     /locus_tag="VFD89_01345"
     CDS             complement(786..2939)
                     /locus_tag="VFD89_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HZJ56871.1"
                     /translation="MQDIVLALAKKLDVREKQVEDTIALLDEGNTVPFISRYRKEVTG
                     GLDDAQLRNLQEELTYLRNMKARREEITRLIDEQGKLDGELTEKINGAASMTELEDIY
                     RPYRPKRRTRATIAREKGLEPLADILLAQELTEGDILEVASSYIDLGKEVTTAEEALK
                     GAMDIVAEVLSDDADLRKAIREYTFDRGLLAVTGDTKEQSVYEMYYEYSEAVKRIVPH
                     RILAVNRGEKEGILSVKIDIDEQGMLSLIQSRVVTNKNSIVFPYIEEIVSDSYKRLIA
                     PSIEREIRNHLTEVAEEQAIKVFGENLNNLLLQPPVKDRIVMGLDPGYRTGNKVAVVD
                     GTGKVLDTGVIYMTLPHHDTGKAKDYLRQLIVKHSVNIIAIGNGTASKETEMVVADLL
                     KEIDDRVYYAIVNEAGASVYSASALGSEEFPEFDAALRSAVSIARRLQDPLAELVKID
                     PKAIGVGQYQHDVNQKRMADTLQGVVENCVNSVGVDLNTASSSLLQYVAGISKTVSKN
                     IVTYRGEKGKFASRKELLKVSKLGPKTFEQCAGFLRIPGGDNILDNTAVHPEAYGATE
                     KLLELRGYSLGDLSQDSLRGLQGATKGWDLKELSATLGLGIPTLTDILEELKKPGRDP
                     REELPQPILRTDVMDINDLKPDMILTGTVRNVVDFGAFVDIGVHQDGLVHISQLADRY
                     VKHPMDVVQVGDIVKVRVLSVDTARGRISLTMRDM"
     gene            3159..3698
                     /locus_tag="VFD89_01350"
     CDS             3159..3698
                     /locus_tag="VFD89_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HZJ56872.1"
                     /translation="MISVTLLGSGGGMPMPGRYLSSMLIGFQSRKILIDCGEGTQVSM
                     RENKTGFKTLDIICITHCHGDHIFGLPGLGRRGIKLTYITDTRPIDTIPAFVSESDLL
                     VCEGTYGDDEDLRKAIENKHMTFGEAAGLAVRGNVKKLWLTHFSPAMDEPELYFKNAT
                     DVFPNAVLGYDGITMDLKF"
     gene            complement(3709..>3985)
                     /locus_tag="VFD89_01355"
     CDS             complement(3709..>3985)
                     /locus_tag="VFD89_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HZJ56873.1"
                     /translation="FNIYYIEDKRIVKSESIPNPGHRPGFLPNFLNNLGVNVIISGGM
                     GGGAAEIFRENGIEVVTGAGGDAKEAAEQYTKGNLEFTGQICDEHNR"
BASE COUNT          891 a         1086 c          847 g         1161 t
ORIGIN      
        1 caaatcgaat tcgttcctga ttctgagtgc agtcaaaata tcatctgccc tgtgggcatg
       61 ggcttttagg ggtatttctt tgtttaatac ctttaccaga atctccatct tcaggtccct
      121 gtccggcatc ttgtcgggat ccgcttttcc cttctccaac ttctccctat aatcctgtgc
      181 cttgaccagg ttctccctga gaatagctgc tgttgccatc cgggtgctgg gtgccttctt
      241 ttgcccgtca tagacccatt tgggattctc cccaaaggca accttcagcg ccaagggctc
      301 cttaaatacc atgtcgtcta cacgcctgcc ataggtttta agggccgcaa actgtccccc
      361 gattacattg gcacttccgg gaccggttac caccgaggta atgccgtttt ctctggcctc
      421 gcgaaagcaa ttgtcctcgg ggtttatccc atctatggcc ctaagttcag gagtagccgg
      481 gtccgtggct tcgtttccgt cggaaccttc aaaccctatc ccatcctccc acatcccgat
      541 atggcagtga gcgtctacta tgcccggcat aacccacata cccccagcgt ctataacctc
      601 tgcgccttcg gggacctcaa tgtcctctcc aatctctagg atcaccttcc cttttatcag
      661 gatgtctccc ttctcgtata ccttctcatt catggtgaga accttaccgt ttttaattaa
      721 aatcatctta ttacccccat ctatgtattt tctaaataac ctcattaatt tagcgaattt
      781 cttaactaca tatccctcat ggtaagggaa atcctgcccc ttgcggtatc tacgcttaaa
      841 accctaacct ttactatatc ccccacctgt actacatcca tgggatgctt tacatatctg
      901 tcagccagct gggagatgtg taccagccca tcctgatgca cccctatatc cacaaaggcc
      961 ccgaagtcca ccacatttcg aacggttcct gttaggatca tatcgggctt taagtcatta
     1021 atgtccatta catcggtcct gagtataggc tgtggcagct cttccctcgg atcccttccc
     1081 ggttttttca gctcctctaa aatgtccgtc agagtcggga tgccaagccc caaggtagcg
     1141 gacagctcct ttaaatccca ccccttcgtc gccccttgaa gcccccttaa agaatcctgg
     1201 gataaatccc ccaatgaata tcccctcagt tctagcaatt tctcggttgc cccataggct
     1261 tcggggtgaa cagcggtgtt atctagtata ttgtccccgc cggggatcct caaaaatcct
     1321 gcacactgct caaaggtttt aggacccaat ttgcttacct ttaaaagctc cttgcgggag
     1381 gcaaacttac ccttctcacc cctgtaggtc acgatgtttt tagatacggt cttgcttatt
     1441 ccggcaacgt attgaagcag ggacgacgag gcggtgttga ggtccacccc aacgctattt
     1501 acgcaatttt ccaccacccc ctgaagggta tccgccatcc tcttttggtt gacatcgtgt
     1561 tggtattggc ctaccccgat ggctttggga tcgattttta ccagctctgc caagggatcc
     1621 tgaagccttc tggcgatgga tactgcgctc cttaatgctg cgtcaaattc aggaaactcc
     1681 tcgctcccaa gggccgatgc ggaatatacc gaggcccctg cctcgttgac aattgcataa
     1741 tagaccctat catctatttc ctttaacagg tctgcaacca ccatttcagt ttccttcgat
     1801 gccgttccgt tacctatagc tattatgttg acactatgct ttactattaa ttgccgcaaa
     1861 tagtccttgg ccttgcccgt atcatggtgg ggcagggtca tatatataac ccctgtatcc
     1921 agcaccttac cggtcccgtc taccacagcc accttgttgc cggttcgata tccggggtca
     1981 aggcccatga ctatccggtc ctttacggga ggctggagga gtaggttgtt taggttttct
     2041 ccgaagacct tgatggcctg ctcttcggca acctccgtca ggtgatttcg gatctccctt
     2101 tcaatggacg gggctatgag ccgcttatag gaatctgaaa ctatctcctc gatataagga
     2161 aatactatgc tgtttttgtt ggtcaccact ctggactgta tcaaactgag cataccctgt
     2221 tcatctatat caatctttac gcttaggatg ccttcctttt cgccacgatt gacggcaaga
     2281 attctgtggg gtactatcct ctttacggcc tcgctatatt cataatacat ctcgtatacg
     2341 gactgctcct tagtatcccc tgtcacagcc aataaaccac gatcgaaggt atattctcgt
     2401 atagcctttc taaggtccgc atcgtcggat aaaacctctg ctacgatatc cattgccccc
     2461 ttcagggctt cctcagcggt ggttacctct tttcccagat ctatgtagga ggaggcaact
     2521 tctaagatat ccccttccgt cagctcttgt gccagcaata tgtctgccaa gggctcaagg
     2581 cctttctctc tggcgatggt ggcccgggtt cgcctttttg gcctgtaggg cctgtagatg
     2641 tcctccagtt ccgtcatgga tgcagcccca tttatcttct ccgtcagttc cccgtcgagt
     2701 ttaccctgct cgtctatgag ccgcgtgatc tcctcccgtc ttgctttcat gttccgaaga
     2761 taagtaagtt cttcttggag gtttcgaagc tgggcatcgt ccagcccccc ggtaacttcc
     2821 tttctatatc tggaaataaa gggaacggta ttgccttcat ctagaagggc tatggtgtct
     2881 tccacctgtt tttcccttac gtccaatttt tttgccaatg ccaaaactat atcctgcata
     2941 agacaactcc tctatattag ctttaaaatt tgattattat atacttggga ggtctttaag
     3001 agtccaaacc aacccgacat ggaaagtatt tgtataattg gtattgtatt taatagcgag
     3061 ttgcatgtca acaccaactg tctattgtat gtgggacttg gctatagtat cctttaggtg
     3121 gatgcattag tattttggct caggggagga aggactaatt gataagcgta accttactgg
     3181 gatccggggg cggtatgcct atgcccggca gatatctttc gtctatgctc ataggttttc
     3241 aatcccgaaa gatactcata gactgcggag aaggaactca ggtatccatg agggagaata
     3301 agaccgggtt taagaccctg gatatcatat gtataaccca ttgtcacggg gatcacatct
     3361 ttggtctgcc ggggctaggg cgaaggggga taaagctaac ctatattacc gatacccggc
     3421 ctattgatac cataccggcc tttgtcagtg aaagcgattt gctcgtatgc gaggggacct
     3481 acggagatga cgaggatctt cgaaaggcta tagagaataa gcatatgacc tttggagaag
     3541 ccgccggatt agccgtaagg ggcaatgtaa aaaagctctg gctcacccac tttagtcccg
     3601 caatggatga accggagcta tattttaaaa atgcaacaga cgttttccca aatgccgtcc
     3661 tagggtatga tggcattacc atggacctaa aattttaacc ctaaggggtc atctgttgtg
     3721 ctcatcgcag atttgaccgg tgaattccag attgcccttg gtgtattgct ctgcagcttc
     3781 ctttgcgtcc cccccggccc ctgttactac ctcaatgccg ttttcacgaa aaatctctgc
     3841 ggctccgccg cccatcccgc cggagataat aacgttcacc ccgagattat ttaggaagtt
     3901 gggcaaaaaa cccggcctgt gccccggatt gggaatcgat tcacttttaa ctatcctctt
     3961 atcctctatg taatagatat taaag
//
LOCUS       DASRNX010000038        31375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_697326,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000038 DASRNX010000000
VERSION     DASRNX010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 31375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31375
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697326"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..128)
                     /locus_tag="VFD89_01360"
     tRNA            complement(52..128)
                     /locus_tag="VFD89_01360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92..94),aa:Arg,seq:tcg)
     gene            complement(134..207)
                     /locus_tag="VFD89_01365"
     tRNA            complement(134..207)
                     /locus_tag="VFD89_01365"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Gly,seq:tcc)
     gene            complement(576..1175)
                     /gene="rdgB"
                     /locus_tag="VFD89_01370"
     CDS             complement(576..1175)
                     /gene="rdgB"
                     /locus_tag="VFD89_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZJ56874.1"
                     /translation="MKRLIIASNNTGKIKEISEIIGSRGFELLSLKDIGFTGEIMEGC
                     HSFKENAIKKALCVMGFAEELTLADDSGLEVDALGGQPGVLSARFSGQDATDESNNKK
                     LLRLLSNIPEGERNAQFRCVMALVHPNGERDIAEGICRGRIADKPKGDNGFGYDPIFI
                     PDGYDKTFAELGDGVKNAISHRSIALEKLRWIMVGKGLI"
     gene            complement(1371..2360)
                     /locus_tag="VFD89_01375"
     CDS             complement(1371..2360)
                     /locus_tag="VFD89_01375"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZJ56875.1"
                     /translation="MFYKKLTLVILFLLIFSIAASGCGIIRKKGNVTPAEEEVPELEG
                     GDTEEEENTRETILFYQDDFGYLVPVMRKIQWEEGIARATLIKLMDTPEQQQAMLEMG
                     LKPLLPANTVINGISINDGLAKVDLNGIAMDYPDAVAENNMVKGVAMTLAEFPAIDRV
                     QLLFDGKIVDTLKYGTHVGDPIKPSEINLELSPESQGNGAEVMVYFHNTSSSRYEYLV
                     PITRITSSPSATLETALDELISGPKLGSNLHFDIPEETKILGVQLDNGITYVNLSKEF
                     EALRDSEHESVVLKAMGLTATQFPEVENLKILVEGVEYKGGTPLSAPAFANEY"
     gene            2691..4457
                     /locus_tag="VFD89_01380"
     CDS             2691..4457
                     /locus_tag="VFD89_01380"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HZJ56876.1"
                     /translation="MALDGVLLNCILTELKDTIIGGRIDKVYQPEKDEVHLGIRCKNQ
                     DRRLLISASPNYPRIHLTGRQKINPITPPVFCMVLRKHLLGGRIIEIEQPDFERILIL
                     SIEARDELGDLSVKRLIVEIMGRHSNIILVDDGELIIDSVKRINKDISRVRRVLPGET
                     YNFPPSQNKHNPLTQDINTLENILNMAFEPKRIAGIILNNFTGISKVTAQEIVYRAMP
                     EDPDDLNPAGGSCAWEKLCVSFITFFEGVKKMDYDPSLLMDETGRPLDIFPFPFYQFP
                     TRLQKRFSSVSVALDSFFETRDSVERAHQRTSHILRVLNTNLNRVQKKHRVLLDEYGE
                     AKNADIYRLFGELITANLYQIPKGAVDVELVNYYDPEGKSVMVPLDKGKTPNQNAQSY
                     YKKYNKAKNALIMINRQLKDARAEIQYLENQLDNLDKCTEDPEIQEIHDELVEEGYIR
                     LRNRKKPQKGSIKSKPYHFVSSDGFDIFVGKNNTQNDRLTLRTAGPNDLWLHVKEIPG
                     SHVVVKSQGQNIPDKTLLEAGVLAAYFSRARDSSNVPVDYCPRKNVRKPSGAKAGMVI
                     YDNYKTMFVTPERGIMDRLKGV"
     gene            complement(4464..4763)
                     /locus_tag="VFD89_01385"
     CDS             complement(4464..4763)
                     /locus_tag="VFD89_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56877.1"
                     /translation="MLDSILEHRVLRDGLYDISLIMNENDYFMVYDNISNENATEIMD
                     NYLVRRQDDGRPDGVQIKHNKNRHIVSINASLHYTENDKTTYTMRPHGYLNNDVE"
     gene            complement(5180..5518)
                     /locus_tag="VFD89_01390"
     CDS             complement(5180..5518)
                     /locus_tag="VFD89_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HZJ56878.1"
                     /translation="MSKYAIFAFQGELMCFQHVLLNVLDLQSKGYESKVIIEGEAVKL
                     VKDLEESGNKLYKRAKEMGLIAAICKACSAKLGVLDYNKNVGIPMGDEMGGHPAMSTY
                     IEQGYEIITL"
     gene            complement(5572..6150)
                     /gene="pyrE"
                     /locus_tag="VFD89_01395"
     CDS             complement(5572..6150)
                     /gene="pyrE"
                     /locus_tag="VFD89_01395"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZJ56879.1"
                     /translation="MTDREEILQIFKKTNALMEGHFQLTSGLHSDKYMQCAKVFQYPE
                     FAEVLARQLALSFEEDGVEIVIGPALGGIILAYEVARSIGVKNIFAERENGIMVLRRG
                     FELPKNTRVLVVEDVVTTGGSVREVMELVASMGSMVVGVGSLVDRSQGLADFGVKFRA
                     LLSMKVQSHAPDSCPLCLLGIPIVKPGSRKIV"
     gene            complement(6251..7171)
                     /locus_tag="VFD89_01400"
     CDS             complement(6251..7171)
                     /locus_tag="VFD89_01400"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HZJ56880.1"
                     /translation="MKELDLNIDIAGVHFKNPIIAASGTYGFGLEYERYMDLNQLGGI
                     AVTGLTLEPRKGNPPPRIAETPSGILNSVGLQNPGIDLFLEKYLPILKEYKTVIIANI
                     NGNTPGEYSELASRLADSSVDMVELNLSCPNVREGGIAFGTDPKTVYAITKEVKQRVR
                     HPLIVKLTPNVTDITQIALAAQEGGGNAISLVNTFLGMAIDAKTRKPILANITGGLSG
                     PAIKPLALRMVWQVSGAVKIPVIGMGGIMSGEDTAEFLLAGATAAAVGTANLVSPNAM
                     MEILDGLKAYMTENGVPRIKELIGSLRYSV"
     gene            complement(7168..7947)
                     /locus_tag="VFD89_01405"
     CDS             complement(7168..7947)
                     /locus_tag="VFD89_01405"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HZJ56881.1"
                     /translation="MLRYEVRVLENYEIANFIYKMTLDAGEIAQRAQPGQFVHIKIPE
                     DASLLLRRPISINGVDLTTGQVDLIYQVVGKGTNILSGVKKDTKLDVLGPLGRGFWIP
                     EGCMKLYIVGGGCGVAPIKFIGQRFGHLDITSFLGFRSRSAAYQIQDFESFSREVFIS
                     TDDGSMGEKGPILHSLRHKLSHERPDIILACGPVPMLKILQSFVAEHDIPCQISLEER
                     MGCGVGGCMVCSCEIGDDGHRDYKKVCADGPVFWSEEVLLG"
     gene            complement(7941..11162)
                     /gene="carB"
                     /locus_tag="VFD89_01410"
     CDS             complement(7941..11162)
                     /gene="carB"
                     /locus_tag="VFD89_01410"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZJ56882.1"
                     /translation="MSLNRDIKKVLVIGSGPIIIGQAAEFDYAGTQACIALREEGIEV
                     VLVNSNPATIMTDPNMASRTYIEPLTASVIKKVIVKERPDSILPTLGGQTALNLAMEL
                     AEDGFLEAQGVRLLGTSMDSINKAEDRESFKRAMMRIGEPVVESSMATDLDMATAFAQ
                     EVGYPVVVRPAYTLGGTGGGMASNREELTDIAKEGIRLSRVGQVLIERSIAGWKEVEF
                     EVVRDGDGNCIIVCHMENIDPVGIHTGDSIVVVPSQTLGKEEYEMLKRASINIINSLE
                     IQGGCNVQYAVDPHSLDYYVIEVNPRVSRSSALASKATGFPIARVATKIALGYMLTEI
                     EGNNHRDNCASYSPDLDYIVVKIPKWPFDKFVKADKTLGTKMKATGEIMAIGHCFEEA
                     LLKAVRSLETGMEGLGLFAESLQSLSHEALNSRLSENTDERLFVVSEAIRRGIPLGYI
                     QKSTRIDNFFLTKIHTILSMERELYAKIPGSLDKESILKAKKLGFSDNTIAKLCGVPT
                     GIIKKMRDRYGIKPSYGIIKPYARRGNRAGEYHYSTYNLELGPSACRNNTIIVLGSGS
                     IRIGQGIEFDYCSVHCIQALEKMGYETVIINNNPETVSTDPHISHRLYFEPLTPEDVI
                     GVLEKEKPLGVIVQYGGQTAIKLAKAVQDAGYDIIGTSLKSIHQAEDREEFNRLLSRL
                     DIPQPKGTTVFTTHEAQSAARALGYPVLVRPSYVLGGQGMEIAYSDDDIAEYMAIINL
                     SRQEHPILIDKYIMGNEIEVDAISDGEDVLIPGIMEHVERAGVHSGDSISVYPPQGLS
                     GGVVEQIVDYTRRLARALGVLGLVNIQFVEHGGRVYVIEVNPRSSRTVPYLSKVTGIP
                     MVKLATEVSLGYPLKKMGYGMDLHKAQGKTTVKVPVFSFEKLPLVDTGLGPEMKSTGE
                     VLGVGKTFSEALYKGMLAAGYNFPKTGSVLLTISDTHKQELIPIASRFFKLGFKIYAT
                     SGTALQLHSNYIPANVLRRIGEREPNIEDYIKEGKINIIVNTPTKGRVQERDGFRLRR
                     MAVEHSIPCMTSLDTANAFLDTIELRMGEDGLEIYPMEEIDKC"
     gene            complement(11153..12127)
                     /gene="pyrF"
                     /locus_tag="VFD89_01415"
     CDS             complement(11153..12127)
                     /gene="pyrF"
                     /locus_tag="VFD89_01415"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZJ56883.1"
                     /translation="MDKLIEKIVQKRNPTVAGLDPRLEYLPEELINKHFKDVIDMRAA
                     ARAILEFNKVILDNIHDIVPAVKLQIAYYEMYGLAGLETFIKTAEYAKSKGLIVIGDV
                     KRNDIGSTAGAYSNAFIGRTPLRDQYTRAFPVDIITVNPYFGTDGIKPFLDDCRRYNR
                     AIFVLVKTSNPSSCEFQDLITVDGSRIYEEVGKRVNEWGSDLVGEHGYSRVGAVVGAT
                     FPHQLVELRETMRGVHFLVPGYGAQGGSVNDVLGGFDNRGLGAIINASRSIMCAYLSE
                     TWRDKFGPQDFGAASRAEVIKMREEILGGLRAKGFDDYRFHQGGDTCL"
     gene            complement(12127..13413)
                     /locus_tag="VFD89_01420"
     CDS             complement(12127..13413)
                     /locus_tag="VFD89_01420"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZJ56884.1"
                     /translation="MLICIHNGNIIDGKGGIFLGKDILIRGSKIIEIGAGLSAGAHKI
                     IDATDYWVLPGLIDAHCHLREPGFEYKEDIVSGTRSAAAGGFTGVACMPNTLPAIDNA
                     AMVQYIKLKAASEGIVRVHPIGAITQQRKGDALVEMGDMKEAGVIALSDDGDPVSNPN
                     TMRLALEYARQFGLLIISHCEDKSLVADGVINEGYISTILGLKGITRAAEEVMVARDV
                     LLSEVVDTPVHIAHVSTKGSVEIIRQGKARGVPVTCETAPHYFSATDGWVEGYDTNTK
                     VNPPLRTDEDVAAIREGLRDGTIDIIATDHAPHHRDEKEMEYDLAEFGISGFETAFSL
                     SYTHLVDTGILTLEELSGKLSTIPAKILGIQGGILDTGLPADITIVNTGMEYEIDRDS
                     FFSKGKNTPFHAKKVKGKVVYTMVDGKIVFNEEVIP"
     gene            complement(13394..14341)
                     /locus_tag="VFD89_01425"
     CDS             complement(13394..14341)
                     /locus_tag="VFD89_01425"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZJ56885.1"
                     /translation="MALKQKDILSLRALSKEEILEILDTAELMKYILKQNNKKTPHLH
                     GKSVVNLFYENSTRTRLSFELASKYMSASSANISTSSSSVKKGEGLIDTVRTIARMGA
                     DVIVMRHPMSGAPHIAAKHVGASIINAGDGMNEHPTQALLDMFTIRESKIDLNGLKVT
                     IIGDIAHSRVARSNIWGLSKMGARVEVAGPSTLLPPELHRTGIRIYNRVEEAIKGADV
                     VIVLRLQKERQQTGLLPSLREYTQIFGLNKERLKLAREDVLIMHPGPINRGIEIASDI
                     ADGLHSRIDEQVTNGVAVRMALLYLITRQRGEQNANLHP"
     gene            complement(14496..15740)
                     /locus_tag="VFD89_01430"
     CDS             complement(14496..15740)
                     /locus_tag="VFD89_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009422719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HZJ56886.1"
                     /translation="MEPITQETRLSPWKIIILGIQHVFAMFGATVLVPALTGLDPSVA
                     LFTAGVGTLIFHLVTKGKVPVFLGSSFAFIAAIAAVVANQGIAYAGGGIIAAGLLYLL
                     LAGLVLIFGVDRIRSFFPPLVTGPMIMVIGLTLSPSIIANNIVGVEGSDVLAMGTLGQ
                     RWIIVLAVIVTMIIVSIFVKGFFKLVPILLGIIVGYIVSILFRFVDFTVIKEASIFAL
                     PPFTLPKFDIKAISLIAPIAIVTFMEHIGDITTNGAVVEKDFFKDPGLHRTLIGDGLA
                     TMFAGLLGGPSNTTYSENTGVLAVTKVYDPFILRVAAVFAIVLGLIGKIGAVLKTIPA
                     PVMGGVSILLFGMIASIGLRTLSDAKIDFTKNRNLIVISLMLVMGLSGVEFTIVQGAT
                     LSGMSLAAIIGVILNKILPENI"
     gene            complement(15875..16417)
                     /gene="pyrR"
                     /locus_tag="VFD89_01435"
     CDS             complement(15875..16417)
                     /gene="pyrR"
                     /locus_tag="VFD89_01435"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HZJ56887.1"
                     /translation="MLEKAQIMDEAGINRALTRIAHEIIERNKGIQGVVLIGIQRRGV
                     PLAYRLAQNIKEYEGVKIPVGILDITLYRDDLSTLAEHPVINSTDIPFDINGKTVVMV
                     DDVLFTGRTARAAMDALIDLGRPTSIQLAILIDRGHRELPIRADYVGRNLPTSRSEIV
                     NVQVVEVDKINKVTIAEITL"
     gene            complement(16604..17479)
                     /locus_tag="VFD89_01440"
     CDS             complement(16604..17479)
                     /locus_tag="VFD89_01440"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZJ56888.1"
                     /translation="MRLDIFLSELIAEESRSSIQRLIKDGMVTVDHRETKPNYKIKTG
                     QNIRVSIPSPVKVAIVPENMDLDIIYQDSDIAVINKPQGMVVHPAPGNYSGTLVNALL
                     YHCDRLSSINGKIRPGIVHRLDKDTSGLILVAKNDYAHRHLAAQIAGRTALRVYSAIV
                     ENNFADEEGTIDAPIKRHPVERKKMTVMPGPGSREAITHFRVMERFGEFTLIEASLET
                     GRTHQIRVHMSHIRHPIVGDPLYGSKKQRFNLPGQALHAKMLGIIHPTTGKFMEFIAS
                     PPEYFVELVARLRSI"
     gene            complement(17538..17999)
                     /gene="lspA"
                     /locus_tag="VFD89_01445"
     CDS             complement(17538..17999)
                     /gene="lspA"
                     /locus_tag="VFD89_01445"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZJ56889.1"
                     /translation="MVQILIIFGIVLLDRISKYLAIVYLKDIGSIEIIKGVFSLTYVE
                     NRGAAFGILQNQRWFFIILTVVVGIGIIYFMFTQSNIESLLRISLSMILAGAIGNLID
                     RIRFGYVVDMLHFTLIDFPVFNVADCFVVAGTILLAFYTLFIADKPEKEVV"
     gene            complement(18140..18817)
                     /locus_tag="VFD89_01450"
     CDS             complement(18140..18817)
                     /locus_tag="VFD89_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HZJ56890.1"
                     /translation="MKDHEINYLRDKLISEREIASQRLRVNREFIGESNIKEDLNELS
                     SYDNHPADHGSELYEKEKEYALETHNISYLKSIEDSLTKIENGEYGVCESCGKDIDFE
                     RLNAHPVAKLCIECQDNPSNSTDSTDLDDDRPIEESVLGYPFGRTFTDDTDNVVYDGE
                     DAWQDVQGYGSSSGPQDISVNRLIDYGNVYYGSEERVGLAEKMDLVDNKDYKKQLPDI
                     DKGDKKT"
     gene            complement(18912..19397)
                     /locus_tag="VFD89_01455"
     CDS             complement(18912..19397)
                     /locus_tag="VFD89_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZJ56891.1"
                     /translation="MSLTPMDIHNKEFSKSFRGYDDEEVDQFLDEIIDEFERLYKENI
                     ELKERLGMLADQISNYKTMENTLKETLVTAQRAASDVLESSQKRSDLIIDEAESKGRK
                     IIQDANDRVILIRREFDEYRKQIQVFKTRFKSLLETQLEVINQEIPTYEGMDGDGLPS
                     G"
     gene            complement(19847..20323)
                     /gene="sepF"
                     /locus_tag="VFD89_01460"
     CDS             complement(19847..20323)
                     /gene="sepF"
                     /locus_tag="VFD89_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HZJ56892.1"
                     /translation="MSNKIFNKLLNIIGLEENILEEVEETVIEDDLNRDNDRLIEPNF
                     KSRNKKGKVVNIHTSTYVKVVVYQPLSFDDTQNIIDNLKSRKPIVVNLDSLEADLAQR
                     VLDFMSGAVYSLDGTIQKVSRGIFVLAPSNVDIVGNIPEELKGKSFFTLSNKKIDD"
     gene            complement(20355..21038)
                     /locus_tag="VFD89_01465"
     CDS             complement(20355..21038)
                     /locus_tag="VFD89_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZJ56893.1"
                     /translation="MTIEEKIQLVRDGIYEAALRVNRKPEDISVIAVSKNVPIYRIDE
                     AAEAGITQFGESRVQEILGKYESVQKNIDWHFIGPLQTNKVKYIIDKVRLIHSLDRTT
                     LAKEINKRAKQNNRPVQVLVQVNLPRENTKSGVYEEDLHPFIESLANYPYIKVKGLMA
                     IGPNTPDKEAIRTCFRRLRFHFDALRESDISHIDMQYLSMGMTNDYMIAVEEGANMVR
                     IGRAIFQDT"
     gene            complement(21341..22546)
                     /locus_tag="VFD89_01470"
     CDS             complement(21341..22546)
                     /locus_tag="VFD89_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZJ56894.1"
                     /translation="MTRGIIIGNRRMKLRFLVFLIITLLGLSYLISLTLKRPLYDVIQ
                     YGEITTWDKCQGIIVRDEKPYFAPAYGKVDFCISDGEMVEENDPIAILFKDNYNKMIM
                     EDLYKIRQKISDYQNKNLIQDLIDTDYDRVQEDIEEILHAIQYGSVSGAISEMGRHEY
                     QLKGLLSERKAILDKRELSNNYLDHLLTQENDMKEILDEWRVDIISPETGFISFKLDG
                     LETQLTPGTVDLLTLEGYMSFLEHQVPDYNNNEIIAGVPLFKVVIPDKWYIISLIPNK
                     NVLYERNDSVKITLPGSADHELMGKVYKTEYLADSVLIALEMTDDVVDILGARNVHLE
                     IGTQIKGLMIPNKAIVKKRDKTGINVLRDGKIQYLEIQVQATDGERSIISRMNETDIL
                     DLNDKILVN"
     gene            complement(22683..23108)
                     /locus_tag="VFD89_01475"
     CDS             complement(22683..23108)
                     /locus_tag="VFD89_01475"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ56895.1"
                     /translation="MRLLFKKQIVGDSPPAVRDLISEMMACIMEWNHLKEEEYYEFRL
                     ILNELISNGIFHGNKGLCNKKVNVAIKEVNPTTLDIFIKDEGQGFDYKRICQGYLGDD
                     ILKLSERGRGLMLVNALCSKIQFDANGSHVKITKSICPR"
     gene            complement(23229..23990)
                     /locus_tag="VFD89_01480"
     CDS             complement(23229..23990)
                     /locus_tag="VFD89_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HZJ56896.1"
                     /translation="MCGQINNTIKTENGETLLRQYSETRDIGLRNELVNHYLYIAEII
                     AKKFVNRGIEYDDLFQVASLALIKALERFDINRGYKFSSFATPTIIGEIKNHFRDKSR
                     IIRLPRREGEMIKKVEDARTYLSNQLGRSPKIEEIGEYLKVTPEEVLQLMESSYLTNT
                     ASLDYYIDDDNETDLMAMIGREEKNYSRVEDRDIIEKIMAGLGDTEKKVLYERFYNER
                     SQKEIAEEIGVSQMYISRMERRLLERFRSYIKRTI"
     gene            complement(23998..24390)
                     /locus_tag="VFD89_01485"
     CDS             complement(23998..24390)
                     /locus_tag="VFD89_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ56897.1"
                     /translation="MSSDLITLAIPAKPEYVMIARLSSAAVATRIGFDVDQIEDIKMA
                     VAEAIIMIINQLTRPEKLSISFILDPKVIGIEIFGEGDWESSNISTHDLGQAQLGKYI
                     LSSMMDSVKFDLDNGTIRAIFMHKDLGV"
     gene            complement(24380..24706)
                     /locus_tag="VFD89_01490"
     CDS             complement(24380..24706)
                     /locus_tag="VFD89_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZJ56898.1"
                     /translation="MILDERFDSDTKMWYISLDGEIDIYNAPEFKEKLHQLIQQNPGD
                     FILDCDSLNYIDSTGLGVLISALRRVKEYNGEIKIRNLKPYIYKIFVITGLDKIFTIE
                     VGNREQ"
     gene            25062..25138
                     /locus_tag="VFD89_01495"
     tRNA            25062..25138
                     /locus_tag="VFD89_01495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25096..25098,aa:Pro,seq:tgg)
     gene            25159..25233
                     /locus_tag="VFD89_01500"
     tRNA            25159..25233
                     /locus_tag="VFD89_01500"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25192..25194,aa:Glu,seq:ttc)
     gene            25241..25317
                     /locus_tag="VFD89_01505"
     tRNA            25241..25317
                     /locus_tag="VFD89_01505"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25275..25277,aa:Ile,seq:cat)
     gene            25338..25414
                     /locus_tag="VFD89_01510"
     tRNA            25338..25414
                     /locus_tag="VFD89_01510"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25372..25374,aa:Asp,seq:gtc)
     gene            25422..25497
                     /locus_tag="VFD89_01515"
     tRNA            25422..25497
                     /locus_tag="VFD89_01515"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25455..25457,aa:Phe,seq:gaa)
     gene            25518..25605
                     /locus_tag="VFD89_01520"
     tRNA            25518..25605
                     /locus_tag="VFD89_01520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25552..25554,aa:Leu,seq:cag)
     gene            complement(25672..26445)
                     /locus_tag="VFD89_01525"
     CDS             complement(25672..26445)
                     /locus_tag="VFD89_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HZJ56899.1"
                     /translation="MHWSYGDFTKAFFKLTQIDLSCYKERQMKRRIDSLISRHNYVHY
                     EDYYRALNADKTMLGNFIDYITINVSEFYRNANQWETLRKDIIPMLLKKNNQLKIWSA
                     ACSTGEEPYSLAMLLQDYLSLNRVKIIATDIDREVLRKAREGVYLEKSLQSLPKSYVK
                     KYFTMEDGLYIIDEKIKKQIEFRQHNLLMDPYPTDCHLIICRNVMIYFTEEAKNQLYP
                     KFRESLEVGGVLFVGSTEHIIFPQKYGFESVKTFFYKKI"
     gene            complement(26656..28128)
                     /gene="rpsA"
                     /locus_tag="VFD89_01530"
     CDS             complement(26656..28128)
                     /gene="rpsA"
                     /locus_tag="VFD89_01530"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZJ56900.1"
                     /translation="MNVFEGNNNEKINDVQNDSQGIDNETPIPEEINEEPIKAQEDNS
                     SAVEIETAESPISESVDENEESPVDVQEQDISQEEEESIEGQESDSAEESEEDSEDDN
                     SEEITTMEGFEETMVTLRSGQLIKGNVLSVNESEVIVNIGYKADGIIPLNELILDGDQ
                     TPLDIFSPGDEVEVEVLKVKDEEGNVILSRKSVERKKAWKAIEQGLETGEEFEGVCTE
                     SVKGGVIARIGEIRAFVPASQLSNRYIEDLSIFVGQTLRLKIIELERRRRRVVASQRV
                     ILEAEEEEKRQKLWASIEEGQRIQGVVKRLTNFGAFVDIGGVDGLIHISDLSWGHVNN
                     PREVLSEEENVEVIVLAADREKERISLGYKQTLPHPWDNVEEKYPAGSVVNGKVVRIT
                     SFGAFIELEPGVDGLVHISQVANRRVQKVEDELSIGQQIDVKVLDANSDSRRISLSIR
                     KLLPDERVKPESTRPKAKEDQFQKEDMTVSLGEFFPEDFN"
     gene            complement(28681..29268)
                     /locus_tag="VFD89_01535"
     CDS             complement(28681..29268)
                     /locus_tag="VFD89_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZJ56901.1"
                     /translation="MFYKIIRGLLRPILFLLYRPKTIGLDNFPKTGKVVLYSNHISML
                     DPILIACMLPRRIYFMAKSELFQNPILRFVLKNVGAFPVKRGTADLAAIKNSLKVLKG
                     GNVFGIFPEGTRGKQGEVKHFSLGAASIAHRSKAKVIPVGVIGRYKLFHPMEVVVGEV
                     LDMDNYFSQKSNTELMELISTEMENSLKSLFNKQG"
     gene            complement(29282..30340)
                     /gene="cmk"
                     /locus_tag="VFD89_01540"
     CDS             complement(29282..30340)
                     /gene="cmk"
                     /locus_tag="VFD89_01540"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZJ56902.1"
                     /translation="MFFYAIRGAITADDNTSPQILDNTEMLLKEIIKRNNIEYSEIVN
                     IIFTGTRDLDSIYPAVAARELGMVDIPLICCQEMHVEGSLEKCIRIMMHIQSPLSRTI
                     EHVYLKKAKSLRPDLSRDNVSKTISDQPFTVAIDGPAGAGKSTIAKALSKKLGIIYLD
                     TGAMYRAIAYKMLNADIGFNNTQGIVSALASTEIEIEFNNGNQGIVLDGIDVSDKIRC
                     PEVSMGASRVAVLPEVRIRLAKIQRDIAKSNSLVMDGRDIGTYVLPDATLKFFLTASL
                     EERARRRWEEMRLRGYEESIEAVKEDIRARDSVDENRSFAPLEVPKDAITIDTTRKSI
                     GEVLAEMLGHTERYCRKG"
     gene            complement(30354..30767)
                     /locus_tag="VFD89_01545"
     CDS             complement(30354..30767)
                     /locus_tag="VFD89_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HZJ56903.1"
                     /translation="MDLLGSREVARIAIQLSLTKNRAEENFHKEEYLKTGIRTVAIDY
                     GGDFITSVNKIIERAVVASKREGVIGSSHAEEGAIAGATREAITQIMPKAMGLSVGGK
                     IAVARYHDHVSVALFLGIGLLHLNEVAVGLGHRAI"
     gene            complement(30841..>31375)
                     /locus_tag="VFD89_01550"
     CDS             complement(30841..>31375)
                     /locus_tag="VFD89_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56904.1"
                     /translation="YIIVPDLFFSTLKGFTDEIMRTRKKHPNIGTIHIITERIETHLS
                     RLGLALAIVLLVLQVYHYYSAFYGAHNPLYSMGSGVGPISRSEYQQVLIHIETAIGEG
                     DEVLIRVNGRIIDSVQNQHPLFVNLLSKDMLEIDAREIRTPGQIVINSEPTQTHPEGY
                     TQKLNIDGELYRLSPIK"
BASE COUNT         8434 a         7514 c         5828 g         9599 t
ORIGIN      
        1 ggtgctctac cgactgagct aatggcccct atcgataata cttcagttta atggcgcgcc
       61 cagagggatt cgaacccccg acccccagat tcgaagtctg taactctatc cagctgagct
      121 atgggcgcat acttggagcg ggtgatgggg atcgaaccca cacggccagc ttggaaggct
      181 ggaattctac cattgaacta cacccgcaaa tcggctaaca attaaaaatt ttaccataaa
      241 atcaatagaa tgtcaatatc ttaattatta ataggttgcc gattcgtacg acagcgaatt
      301 ttagtataaa ccaaatttgc catctcgtca agtccaattt ttaaaattga atatactgca
      361 actttaagtc aatcgctagt gtagcatccg gccctagagg gtacaacagg tgatactttt
      421 gcgttcaata ttatgattaa gatgtcaaaa ctacatttca ctaaaaaata tgctttatat
      481 agcggttgac ataataatca gtacgatata tagtaccata aattggtatt atgacctttt
      541 gacatttgaa tcatattatt agtatcatac agcaattaaa taaggccttt ccccaccata
      601 atccatctta atttttctag cgcgatggag cggtggctaa ttgcattctt tacaccatcc
      661 cccagttctg cgaaagtctt gtcatagccg tctggaataa atattggatc atagccaaaa
      721 ccattatccc ctttgggctt gtccgcaatt cttccacgac aaatcccctc cgcgatgtcc
      781 ctttcgccat taggatgcac aagagccatt acgcatctaa actgggcatt cctttcccct
      841 tccggaatat tacttaaaag cctgagcaat ttcttgttgt tgctttcatc tgtggcgtct
      901 tggcccgaaa atcgtgcaga cagaaccccc ggttgtccgc ctagggcatc tacttccagg
      961 cctgaatcgt cggccagtgt caattcctcg gcgaatccca taacgcacag ggccttcttt
     1021 atagcattct ctttaaagct atgacatcct tccataattt ctcctgtaaa cccaatatcc
     1081 tttaggctaa gcaattcaaa tcctcgtgaa cctataattt cgcttatctc tttgattttc
     1141 ccagtgttat tggatgctat tattaacctc ttcataatgc tccttttatg attaatgata
     1201 cagatcccat ggttctccca gaaagcaata aaggtcttga tcacatacga tacccatatt
     1261 agatcgtaat acgccaaagt tatatcgtat attaagtaca cctgtagtac cttctgggat
     1321 aaaaccatta tacagcattg tttaatacat taaaagtata ttggaaaatt ctaatattcg
     1381 ttggcaaacg ccggtgcgga caaaggtgta ccccccttat attctacccc ttcaactagg
     1441 atttttaggt tttccacctc cggaaactgc gtagcggtga ggcccattgc ctttaatacc
     1501 acggactcat gttcggaatc cctcagagcc tcaaattcct tgcttaaatt gacataggtt
     1561 ataccgttat ctaattggac ccccagtatt ttggtttctt ccgggatatc aaagtgaaga
     1621 ttggatccaa gctttggtcc gcttatcaat tcatccaaag cggtttctaa agtggcggat
     1681 ggactcgatg ttattctggt tattggtaca agatattcat acctgctgga ggatgtattg
     1741 tggaaataca ccataacctc cgcaccattg ccctgggact ccggcgacaa ttccaaattt
     1801 atttcactag gcttaatagg atcacccacg tgggtaccgt actttaaggt atccactatc
     1861 tttccgtcga atagaagctg tacccggtct atagcaggga attctgctag ggtcatggcc
     1921 acccctttaa ccatattgtt ctctgcgacg gcatcggggt aatccatcgc aataccgtta
     1981 aggtctacct ttgctaaacc atcgttaata gaaattccgt ttatcacagt atttgcaggt
     2041 aataagggct ttaagcccat ctccagcatc gcctgttgtt gttcgggtgt atccatgagt
     2101 ttaattaggg tagctctagc aattccctct tcccattgta tttttctcat caccggtacc
     2161 agatatccaa aatcatcttg gtaaaacaaa attgtttccc tggtgttctc ttcctcttca
     2221 gtatctcccc cctcaagttc cgggacttct tcctctgcag gcgtaacgtt gcctttcttt
     2281 cgtattatcc cgcatccgga agccgctatt gaaaaaatta gtagaaacaa tattactaaa
     2341 gtcaactttt tgtaaaacat ggtctccccc ctcaccaata tcttggtatt atttatatta
     2401 aaaattggga tggagtatgt ctataggtct aataaattta tcgaatacgt tgcagtggat
     2461 attgctatat atgttacgga aagatttttt acaaatttag ttaaattatg tggtactctg
     2521 ccaaaacaat gtattttccg taacatatga attggggata ttgtttggtc aattgagcga
     2581 tttgtttttt atttacccat tgtttcttat gcgtttgtta aaatatcggg attgggattt
     2641 gttatccgcc aaaaataatg tataatggtt ctctgtagga gggaaaaaat atggctttgg
     2701 atggcgtttt gttaaactgc attctgacgg aattaaagga tacgataatt ggggggagga
     2761 ttgacaaggt atatcaaccc gaaaaggatg aagtccattt ggggattaga tgtaaaaacc
     2821 aggacagaag actcttaatt tctgcaagtc ccaattatcc cagaattcat cttactggga
     2881 gacaaaaaat caatccaata accccgcctg ttttttgcat ggtattgaga aagcatctat
     2941 taggcggtag aatcatcgaa atagaacaac ccgacttcga aagaatacta attttatcca
     3001 tagaagcccg ggatgaatta ggtgacctat cggtaaagcg gttgatagtc gaaataatgg
     3061 gcaggcacag caatatcatt ttagtggacg atggagaatt aattatagac agtgttaagc
     3121 gtatcaataa agacataagt agggtgaggc gggtcctgcc cggagaaaca tataatttcc
     3181 ccccctccca aaataagcat aatcccctga ctcaggatat aaacaccctg gaaaatattt
     3241 taaacatggc ctttgaaccc aaaagaatcg caggaataat attgaacaac tttaccggga
     3301 tatctaaggt tactgcccag gagatagtat atcgcgctat gcccgaggat cccgatgacc
     3361 ttaatccggc cgggggttct tgcgcctggg aaaaactatg tgtttcattt ataacctttt
     3421 ttgagggagt aaaaaaaatg gattatgatc cctccctgct gatggatgaa accggacggc
     3481 cattggacat atttcctttt ccattttatc aatttccgac gagattacag aaacgcttta
     3541 gctctgtgtc cgttgcattg gattccttct ttgaaacccg tgacagtgta gagagggccc
     3601 atcagcgtac ttctcatatc ttaagggtac tcaacacaaa tttaaatcgg gttcaaaaga
     3661 aacacagagt cttattggat gaatatgggg aggccaagaa tgcggatata tataggctat
     3721 tcggtgaatt gattacagca aatctctacc agatccccaa aggcgccgta gatgttgaat
     3781 tggtaaatta ttatgatcct gaaggtaaat ctgttatggt ccccttggat aaagggaaga
     3841 cacccaacca gaatgcccaa agttattata aaaagtacaa taaagcgaag aacgccttga
     3901 tcatgataaa caggcaactg aaggacgcaa gagcggagat acaatacctg gaaaatcagt
     3961 tggataattt agataaatgt actgaggacc ccgaaatcca agagatacat gatgaactgg
     4021 ttgaagaggg atatatcagg cttcggaaca gaaagaaacc ccaaaaggga tccatcaaat
     4081 ctaagcctta ccactttgtc tcctccgacg gttttgatat ctttgtggga aaaaacaata
     4141 cacagaacga cagattgacc ttaagaacag cgggacccaa tgacctatgg cttcatgtca
     4201 aagaaattcc cggttcacac gtcgtagtaa aatcccaagg tcaaaatatt ccggacaaaa
     4261 cgttgctgga ggcaggtgtt ttagccgcct attttagcag ggccagggat tcgtccaatg
     4321 taccggttga ttattgtccg cgaaagaatg tacgcaaacc cagcggcgca aaagcaggaa
     4381 tggtcatata cgataattat aagactatgt ttgtaacgcc ggaaaggggt ataatggacc
     4441 gattaaaagg cgtatgatgt cccctattcc acatcgttat taagataccc atggggcctc
     4501 atggtatatg ttgtcttatc attttcggtg taatgcaggc tggcatttat agatactata
     4561 tgtcgatttt tattatgctt tatctggacg ccatcgggtc ttccatcgtc ttgacgccga
     4621 actaggtaat tgtccattat ttccgtagca ttttcattgc tgatattatc atagaccatg
     4681 aaataatcat tttcattcat gatgagagaa atatcataga gcccatccct gagcacccga
     4741 tgctctaaaa tgctgtccaa cataattacc acccttttct tggtatagtc ttaaagccta
     4801 aggttacatc gcgggaaact atcgcctcgg ttattgctgc ccgcatgttt cggcggggcg
     4861 ggcaatttta cccacatcct gtttgtacga cacgagcccg gcagttataa aggtaaaagg
     4921 tattcttccg ccccctttta gcatccccat tatattttgc acccaatata aataataata
     4981 cacaaaaaat cgccgtggca attacattta caaattgtaa tcgccgcggc gaaaatttaa
     5041 ttgatgcgct ataccggttc attgtcttac ccaaggcacc ttaagcaatc cccatgggca
     5101 aaagaccgaa ttaaaagcga tagactcttt taaatccttt ggatcaaatg atttaggaat
     5161 ctacccgtcc tcgaagctgc tatagggtga ttatttcata cccttgctct atatatgtag
     5221 acatggccgg atgcccgccc atctcgtcgc ccatgggtat cccgacattc ttgttgtaat
     5281 ccaatactcc aagcttagca gagcatgcct tacatatggc ggcaatcagg cccatctctt
     5341 tagccctttt atatagctta ttacccgatt cttccaaatc cttaaccaat ttaacggctt
     5401 ccccctcaat gatcaccttt gattcgtaac ctttgctctg taaatcaaga acattgagca
     5461 acacgtgttg aaaacacatc agttcacctt gaaatgcgaa aattgcatac ttgctcaaaa
     5521 atatccaccc cttttttgta tttgcttatc gttacccttc atctgcggtt ctcaaacaat
     5581 cttcctgctt ccgggcttta caatgggtat tcccaaaaga cataacgggc aggaatcagg
     5641 tgcgtgggac tggactttca tagataaaag ggcccggaat tttacgccga aatccgccag
     5701 accctgactt ctgtctacaa ggcttccaac cccaaccacc atagagccca tgctagccac
     5761 caattccatc acttctctca ccgaaccccc ggtagttact acgtcctcca ctaccaatac
     5821 ccttgtattt tttgggagct caaaaccacg tctcaagacc ataatgccat tttctctctc
     5881 tgcgaagatg tttttgaccc cgatacttct ggctacctca taggctaaaa taatcccccc
     5941 taaagccggg cctatcacta tttctacacc atcttcctca aaggatagcg ccaattggcg
     6001 ggctaagacc tctgcaaatt ccggatattg aaacaccttt gcacactgca tgtacttgtc
     6061 gctgtgtaac cccgatgtca actgaaaatg tccttccatt aaagcattcg ttttcttaaa
     6121 tatctgtaat atttcttccc tatccgtcat tttccactcc tcctcatggc attattatac
     6181 tctttagatt ccattttgaa ctgcagaaga tgcgacattg cgttaatcgt gaatacagcg
     6241 gtcgcctgtt ctaaacacta taacgaaggc tccctataag ttcctttata cgggggaccc
     6301 cattttctgt catatacgct tttaagccgt ccaatatctc catcattgcg ttgggagata
     6361 ccaaatttgc ggtacccaca gccgctgccg tcgcccctgc caataaaaat tctgcagtgt
     6421 cctcccccga cattataccc cccatcccta ttacgggtat tttgactgcg ccggatacct
     6481 gccagaccat tctgagagcc aagggcttaa tagcggggcc tgagagcccg cccgtaatgt
     6541 tggcgagaat aggctttctg gtcttggcat caatagccat acctaaaaat gtattgacaa
     6601 gcgagattgc gttccctccc ccctcttggg ctgccagggc gatttgggtt atgtctgtta
     6661 catttggggt caatttaacg attaggggat ggcgaacacg ctgtttgacc tctttggtta
     6721 tggcgtaaac cgtcttgggg tccgtaccaa aagctattcc gccttccctt acatttgggc
     6781 atgataggtt taattctacc atatctacgg atgaatccgc taatctgctt gcaagttccg
     6841 agtattctcc cggagtattg ccattgatat tggcgataat aacagttttg tactctttaa
     6901 gaataggtaa gtacttttct agaaataaat caattccggg gttttgcagc ccgacactgt
     6961 tcagtatccc cgagggagtt tcggctattc tggggggcgg gttgcctttc ctgggctcta
     7021 gggttaatcc agtgacggct atgccgccca attgatttaa atccatatac ctttcatatt
     7081 ccaatccaaa gccataggtt cctgatgcgg cgataatcgg attcttaaaa tggacacctg
     7141 cgatatcaat attgagatct aattccttca tccgagtaaa acctcctcac tccaaaacac
     7201 cggcccatca gcgcatacct ttttgtagtc ccgatgccca tcatccccga tctcacagga
     7261 gcataccatg catcccccga caccgcatcc catcctctcc tctagggata tttggcaagg
     7321 gatatcatgc tccgcaacaa aactttgaag gatcttgagc atagggacag gtccgcaagc
     7381 caatatgata tccggtcttt catgggaaag cttatgcctt agcgagtgga gaattggtcc
     7441 tttttcaccc atactgccgt catccgttga gatgaatact tccctactga aactttcaaa
     7501 gtcctgtatt tgatatgccg cagaccggga acggaacccc aagaaggagg taatatccaa
     7561 atgtccaaag cgttgcccga taaattttat aggggccaca ccgcatcccc cacctacgat
     7621 gtataacttc atacaacctt ccgggatcca aaaccctctt cccaatggtc ctaagacatc
     7681 cagctttgta tcttttttta cccctgacag gatattagtt cccttaccca ccacctggta
     7741 tataaggtcg acctgccctg tagtgaggtc cacgccgtta atgctgatag ggcggcgaag
     7801 caataatgag gcgtcttccg gaatcttaat gtgtacaaat tgacccggtt gggcgcgttg
     7861 ggctatttct cctgcatcca aggtcatttt atagatgaaa tttgctattt catagttttc
     7921 cagaaccctc acttcgtatc ttaacattta tctatctcct ccatggggta aatttctaag
     7981 ccatcttcac ccattctcaa ttcgatggtg tctaggaagg cgttggcggt atctaatgaa
     8041 gtcatacagg gaatagaatg ttcaacggcc attctacgca gcctaaatcc atcgcgttcc
     8101 tgaaccctgc ccttagtggg cgtgttgaca atgatgttga ttttaccttc tttaatatag
     8161 tcttcaatat tgggctccct ctcccctatg cgcctaagga cattggcggg tatataatta
     8221 gagtgcaatt ggagggcggt acctgaggtt gcataaattt taaaccccag cttgaaaaaa
     8281 cgggaggcaa tgggtatgag ctcttgcttg tgggtatcgg aaattgttaa gagcacgcta
     8341 cctgtcttgg gaaaattata tcctgccgcc aacattccct tatacaaagc ctctgaaaag
     8401 gttttgccaa ctcctaacac ctcgccggtg gatttcattt caggtccaag gccggtgtct
     8461 acaaggggca atttttcaaa ggaaaagacc ggcactttta ctgtagtttt tccctgtgct
     8521 ttgtggaggt ccatgccgta tcccattttt ttaaggggat agcccaggct tacctctgta
     8581 gccagtttca ccatcggtat tcctgtaact ttacttagat aaggtacagt cctgctggat
     8641 ctgggattca cttcaataac atataccctt ccgccgtgtt ccacaaattg aatattgaca
     8701 agccctaaga cccccaatgc ccttgccaat cttcttgtat aatccactat ctgctcaaca
     8761 actccgccgg aaagaccttg gggcgggtag accgatatac tatcccccga gtgtacccct
     8821 gccctttcaa catgctccat aatgcccggg attagcacat cttcgccatc ggatattgcg
     8881 tccacttcga tttcattgcc cataatgtat ttatcgatga gaatagggtg ctcttgtcta
     8941 gagaggttga taatcgccat gtattcggct atgtcatcat cgctgtaggc aatttccatt
     9001 ccctgacccc ctaacacata agaaggccgc accaaaacgg ggtatcctag ggccctggca
     9061 gccgactggg cttcatgggt ggtgaatacg gtggtcccct taggctgcgg aatatccagt
     9121 cttgaaagca atcggttaaa ttcttcccga tcctctgctt gatggatgga ttttagcgag
     9181 gttcctatta tatcatatcc ggcatcctgt accgccttgg caagttttat agcagtctgt
     9241 cccccgtatt gtactattac tcccaagggc ttttccttct ccaagacacc tatcacatcc
     9301 tccggggtta agggctcaaa atagagcctg tgggatatat gggggtctgt actgaccgtc
     9361 tcgggattat tatttatgat cacggtttca tagcccatct tctcaagggc ttgtatgcaa
     9421 tggacagagc aatagtcaaa ttctattccc tggcctatgc gaatggatcc ggagcctagg
     9481 actataattg tattatttct gcaggcgctc ggtcctaact ctaaattata ggtggagtaa
     9541 tggtactcgc cggcacgatt tccacgtcgg gcatagggtt taattatacc gtaagacggt
     9601 ttgatcccat atctatccct cattttttta ataatccctg tggggacgcc gcagagcttg
     9661 gctattgtgt tatccgagaa ccctaacttc tttgctttca atatggactc tttatccaaa
     9721 ctcccgggta ttttagcgta cagttccctt tccatactca aaatggtatg gatctttgtg
     9781 agaaaaaagt tatcaattct cgtggatttt tgtatatatc ccagcggaat cccccgcctt
     9841 atagcttctg acacaacgaa taaacgctcg tcggtattct ccgaaagcct tgaatttagg
     9901 gcctcatggg ataggctttg caatgattcc gcaaagagac ccagtccttc catgccggtt
     9961 tccaaggatc gcaccgcttt taaaagggcc tcttcaaaac aatgcccgat agccattatt
    10021 tcacccgtcg ccttcatctt tgttcccaaa gttttgtcgg cctttacaaa cttatcaaag
    10081 ggccatttag gaatcttgac aacgatataa tctaaatcgg ggctatagga agcacagtta
    10141 tcacgatggt tattcccctc tatttctgtc aacatatagc ctagggcaat tttagtagcg
    10201 actctggcta tggggaaacc agtggctttg gaagccagag cactggaacg gcttactctg
    10261 ggatttactt cgattacata ataatccaag gaatggggat ctacagcata ctggacattg
    10321 cacccccctt ggatctctaa tgaatttatt atgttaatgg atgctctttt cagcatctca
    10381 tactcttcct ttccaagggt ttgggaggga acaaccacta tactgtcacc cgtgtgaatt
    10441 ccaacggggt caatattttc catatgacat acgataatac aattcccatc cccatctcta
    10501 acgacttcga attcgacttc cttccagccg gcaatggatc tttctatcaa tacctgtcca
    10561 accctactaa gccgaatgcc ctcttttgct atatctgtaa gctcttcccg gttggacgcc
    10621 atcccacccc ctgttccgcc gagggtgtac gcaggccgaa ccaccacagg atatcccacc
    10681 tcttgggcaa aggctgtagc catgtccaag tccgtagcca tgctactctc aacgacaggc
    10741 tcgccgattc gcatcatggc cctcttaaag gattcccgat cttcagcctt atttatagaa
    10801 tccatggatg ttcccagaag acgcaccccc tgtgcttcca aaaagccgtc ctcggctagt
    10861 tccatggcca gattaagagc ggtttgccca cccagtgtcg gcaatatgct gtccggtctt
    10921 tctttgacaa taactttttt tattactgaa gcggttaagg gctctatata tgtgcggctt
    10981 gccatatttg gatccgtcat tatagtcgca gggttgctat ttaccaacac cacctcaatg
    11041 ccttcctcac gcagagcaat acatgcttgg gtacccgcgt agtcgaactc tgcagcctgg
    11101 ccgattatta taggcccgga accaattact aatacctttt taatatccct atttaaagac
    11161 atgtgtcccc tccctgatgg aatctataat cgtcaaaccc ctttgccctt aggcctccaa
    11221 ggatctcctc cctcatctta atgacctcag cccttgaagc cgccccaaaa tcttgcggac
    11281 caaatttatc cctccaggtt tcacttaaat atgcgcacat tatgctcctt gaagcattta
    11341 ttatggcccc gaggcctctg ttatcgaatc ctcccaatac atcattcaca cttcccccct
    11401 gggctccgta accgggcact aaaaaatgga cccccctcat tgtttcccta agctcaacga
    11461 gttgatgggg gaaggttgca cctaccactg cgcctaccct gctatagcca tgctccccca
    11521 ctagatcaga accccactca ttaacccttt tcccaacctc ttcatagatc cttgagccat
    11581 cgacggttat tagatcctgg aattcacaag atgaaggatt tgatgtctta accaagacaa
    11641 atatggctct attatatcgc ctgcaatcgt ctaaaaatgg tttaatgcca tcggtaccga
    11701 aataaggatt gacagtgatg atatctacag gaaatgccct agtatattga tcgcgcaaag
    11761 gggtacgacc aataaaggca tttgaatagg cccctgcggt agaccctata tcattccttt
    11821 ttacgtctcc tatgactatt aatcccttgg attttgcata ctctgcagtt tttataaagg
    11881 tttcaaggcc ggctagtcca tacatctcgt agtatgcgat ttgcagcttt acggcaggaa
    11941 cgatatcatg gatgttatct aatatcacct tgttaaactc caaaattgcc cgggctgcag
    12001 ccctcatatc aataacgtct ttaaaatgct tgttaatcag ttcttccgga agatattcca
    12061 gcctggggtc aagccctgca accgtaggat tcctcttttg tactatcttt tcaataagct
    12121 tatctatcaa gggattacct cctcattgaa aactattttt ccgtctacca tggtataaac
    12181 caccttgccc tttacctttt ttgcgtgaaa gggcgtgttt ttgccctttg agaaaaaact
    12241 gtctctgtca atttcatatt ccatccccgt atttactatg gttatatcag ccggcaggcc
    12301 tgtatccagg ataccgcctt ggatgcccaa tatcttggcg ggtattgtac tgagcttacc
    12361 cgataattcc tccaaggtta atattcccgt atcgaccaaa tgggtgtagg aaagggaaaa
    12421 agccgtttca aatcccgata taccaaattc cgccaaatcg tactccattt ccttttcgtc
    12481 cctatgatgg ggcgcatgat cggttgctat tatatcgatg gtaccatccc taagaccctc
    12541 ccttatagct gccacatcct catccgttct caggggtggg ttgacttttg tattggtgtc
    12601 gtatccctcc acccaaccgt cagtggctga aaaataatgc ggtgcagtct cgcaggttac
    12661 gggaaccccc ctagccttcc cctgccgtat tatttctact gatccttttg tgcttacgtg
    12721 ggctatatga acaggggtat cgaccacttc agataacagc acatccctcg ccaccattac
    12781 ttcctcggcg gcccgggtaa tcccctttaa gcccaatata gtggatatat atccttcatt
    12841 gataacacca tctgcaacca gggatttatc ctcgcagtgt gaaatgataa ggagcccaaa
    12901 ttgtctagca tattccaaag ccagcctcat ggtattaggg ttggacacgg gatccccgtc
    12961 gtcggacaat gcaattactc ctgcctcctt catgtcaccc atctcaacta aggcatcacc
    13021 ttttctttgc tgtgtgatgg ccccaatggg atgcacccgt actatacctt ccgatgcggc
    13081 ttttaacttt atatactgga ccatggctgc attatcaatt gccggtaaag tgttaggcat
    13141 acatgctacg cctgtaaaac cgccggcagc agcactacgg gtgccggaga caatgtcctc
    13201 cttatattca aagccgggtt ctctgaggtg acaatgagca tcaatgagtc ccgggagaac
    13261 ccaataatca gtagcatcaa taattttatg ggcgcccgca gacaaacctg ccccaatttc
    13321 aattatttta cttcccctta tgagtatatc cttccctaga aagattcccc ctttaccgtc
    13381 gatgatgtta ccgttatgga tgcagattag cattttgttc ccccctctgc cgggtaataa
    13441 gatagagtag cgccattcgg accgcaacgc cgttggtaac ctgttcatca atccgagaat
    13501 gcagcccatc ggctatgtcc gaggcaatct ctatacccct gtttatgggg cccggatgca
    13561 tgatgagtac atcctcccgg gccaatttaa gtctttcctt attcaatcca aagatctggg
    13621 tatattctct aagtgatggt aggagtccgg tctgttgacg ctctttttga agcctgagta
    13681 ctataaccac atcggcacct tttatggcct cttctaccct gttataaatc cttatccccg
    13741 tcctatgaag ttccggaggc agcaatgtag aaggcccggc tacctcgacc cttgcgccca
    13801 ttttgctcag tccccaaata ttgcttctag ccaccctgct atgggctata tcgcctataa
    13861 tcgtaacctt cagcccgttt agatctattt ttgattccct tatagtaaac atatccagca
    13921 aggcttgggt aggatgttca ttcataccat caccggcatt tataatggat gcacctacat
    13981 gcttcgctgc aatgtggggt gccccagaca tcggatgacg cattactatg acatccgcgc
    14041 ccatccttgc tatggttctt accgtatcaa ttaggccctc accctttttt acgctgctgg
    14101 atgaggttga aatattggcg gagctggcac tcatatactt gctggccagc tcaaaggata
    14161 gtcgggtcct ggtgctattc tcataaaaga gattaacaac ggatttgcca tgaaggtggg
    14221 gagtcttttt gttgttttgt tttaagatat acttcatgag ctcggcggta tcaagaattt
    14281 ccagaatttc ttctttgctt aaagcccgca ggctcagaat atccttttgt tttaatgcca
    14341 tgccttcacc actttctgca gtatagcata atctttaagg tgggtttatg catcgttttt
    14401 tcccactttg cagctgaaat tatcccttgt atgggaacag caataaagtt ttccttcaaa
    14461 aaggagtagt aagatttcct tactactcct cagacttaaa tattctctgg caagatcttg
    14521 ttcaagataa cccctataat tgcagccagg ctcatgcccg acagggtggc accctgaaca
    14581 atggtaaatt ctacgccgct taggcccata accaacatca atgagataac tattaaattc
    14641 cgatttttgg taaaatctat tttggcatcg gataaggtcc taagacctat gctggcgatc
    14701 attccaaaca atagaatgct gacaccgccc ataacgggag cgggaatggt cttaaggacc
    14761 gcccctatct taccgataag ccctaaaacg atggcaaata ccgccgctac ccttaggatg
    14821 aagggatcat atacctttgt tacagccaaa accccggtat tttcactgta agtagtattg
    14881 gatggtccgc ctaacagtcc cgcgaacatg gttgccaatc cgtcacctat cagtgttcta
    14941 tggagaccgg gatccttaaa gaagtccttc tctactacag ccccattggt tgttatatcc
    15001 ccaatatgct ccataaaggt aactatggct atcggggcta tgagcgatat agctttgata
    15061 tcgaactttg gaagggtaaa tggaggtagt gcaaaaattg aagcctcctt aatcacagtg
    15121 aaatctacaa atctaaaaag gatggatact atataaccta caatgatccc gagaagaata
    15181 ggcaccaact taaagaatcc cttaacgaat atggatacta tgatcatagt aactataacc
    15241 gccaatacta ttatccatcg ttgccccaat gtacccatgg caagcacatc ggatccttcc
    15301 accccaacaa tattattcgc tattatagac gggcttaagg ttaagcctat aaccatgatc
    15361 ataggtcctg taactagggg cggaaagaaa ctgcgtatcc tatccactcc aaatattagt
    15421 accaaacctg cgagcagaag gtagagtaaa cccgctgcga taattccgcc tccggcgtat
    15481 gcaataccct ggtttgcaac aacagcagca atagccgcaa taaaggcgaa gctggagcca
    15541 aggaacaccg gcaccttgcc ttttgtaact aagtggaaaa ttaatgttcc tacccctgcc
    15601 gtaaataatg cgacagatgg gtctaacccc gtcagcgccg gaactagtac agtggctccg
    15661 aacattgcaa acacatgctg aatcccgagg ataataattt tccatgggct aagccttgtt
    15721 tcttgagtaa taggttccat aataattcct ccttcaaaat agtagaaaaa tcctttgatg
    15781 ttatatatca aggcataaaa aaatgccttg tagttaaact gtcaaagcac aatacgaata
    15841 ctgtgatatc ccttgacagc ctcaccggac tgatttaaag ggtaatttca gctatggtaa
    15901 ctttattgat tttgtcaacc tccaccacct gtacattgac tatctcgctt cgggaagtgg
    15961 gaaggttcct gcctacataa tcagcacgga tgggaagctc cctatgcccc ctgtctatca
    16021 agattgccaa ttggatggag gtcggcctgc caaggtctat caaagcatcc atggctgccc
    16081 tggcggtcct tccagtaaag agcacatcgt ccaccattac cacagttttt ccattgatat
    16141 cgaagggaat atcggtgctg ttaataaccg ggtgctctgc cagggtggag agatcgtctc
    16201 tgtatagggt aatatccaag atgcctacgg ggatttttac cccttcatac tcttttatat
    16261 tctgggctag tcggtatgcc agtggcaccc ctctacgttg aatcccaatc aggacaactc
    16321 cttgtatacc tttatttcgt tcgattattt catgggcaat cctggttagc gcccgattaa
    16381 ttccggcctc atccataatt tgggcctttt ctaacaccat catcacctca ttaaaaaacc
    16441 ttaccataag gtaaggcaac attgactgga agccataatc aagcatacta tactcggctt
    16501 ctgatatgtt ataccttacc ggactcacgg gaccagctta aaggcttttg tttttaaaat
    16561 tataacatat cccgatcacc ctgtccatag aaaattttag tttctatata ctccgaagtc
    16621 ttgccaccag ttcaacaaaa tattctgggg gtgatgcaat aaattccata aattttccgg
    16681 ttgtcggatg tattataccc agcattttag catgcagggc ctgacccggt aaattgaacc
    16741 tttgtttttt tgacccgtac aagggatcgc ccactatagg gtgtctgata tgggacatat
    16801 ggaccctaat ttgatgagtc cttcccgttt ccagacttgc ttcgattaag gtgaattcac
    16861 cgaacctttc cattacccga aaatgggtaa tcgcctcacg actcccgggg cccggcataa
    16921 ccgtcatctt cttcctctcg acgggatgtc gttttatggg cgcgtctatt gtcccctcct
    16981 catccgcaaa gttattttct actatagcgc tgtaaaccct cagagccgtc cttccggcta
    17041 tttgagcagc caggtgtcga tgcgcataat cattcttggc taccaggatc aaaccggatg
    17101 tatccttatc cagcctatga acgatacccg gcctaatctt gccgtttatg ctcgataagc
    17161 ggtcacaatg ataaagaaga gcatttacca gggtacccga ataattgccg ggtgcaggat
    17221 ggaccaccat cccttggggc ttattgatta ccgctatatc gctatcttga taaatgatat
    17281 caagatccat gttttccggc actatggcta cttttacagg ggacggaatt gaaaccctta
    17341 tattctgtcc cgtcttaatc ttgtaattgg gcttcgtttc cctatgatcc accgtaacca
    17401 ttccgtcctt tattaacctt tggatggacg acctggattc ttcggcgatt aattcgctta
    17461 aaaaaatatc caatcgcatt ccgttatgta attgttctac attataaatg agaatcttgt
    17521 ttgctttgcc ggacaatcta tactacctct ttctcgggtt tatctgcaat gaaaagggtg
    17581 taaaatgcca gcaatatagt acccgcaacc acaaagcagt ccgccacatt aaaaacaggg
    17641 aaatcgatta aggtaaagtg tagcatatcc accacatatc caaacctaat cctgtcgatt
    17701 agatttccta tggctcctgc aaggatcatt gaaaggctta tcctcagtaa agattctata
    17761 tttgactgag taaacatgaa atatattatt cctatgccta cgacaacggt taatataata
    17821 aaaaaccacc tttgattttg gagtatccca aaggcagcac ctctgttttc tacataggta
    17881 agactaaata cacccttaat tatctctatg cttcctatat cctttaagta gacaattgcc
    17941 agatattttg aaattctgtc caataagacg attccaaaaa ttatcagtat ttgaaccatt
    18001 tacatccccc cactttatga ccccgtccca ttatagcatg gtaacacaat aaattacata
    18061 gtgtcggtcg attttacatg tagccgtcat taagcctcat tctaaaaata aaattccttg
    18121 ggggtgcatc cccagggaat taggtttttt tgtccccttt atcaatgtcg ggcaattgct
    18181 ttttataatc cttattgtcc accaaatcca tcttctcagc caacccaacc ctttcttcgc
    18241 tgccataata gacattccca tagtctataa ggcgatttac gcttatgtcc tggggcccgc
    18301 tggaactgcc gtacccctgg acatcctgcc acgcgtcctc tccatcatag actacattat
    18361 cggtatcatc agtaaatgtc ctcccaaagg gataacccaa gacgctttcc tctattggcc
    18421 gatcatcatc caaatcggtg gaatccgtgc tatttgaggg attatcctga cattcaatac
    18481 agagtttagc tacaggatgc gcgtttaatc tctcaaagtc tatatcttta ccgcaggatt
    18541 cacatacacc gtattcacca ttttcgattt ttgttaatga atcctctatg ctcttcaaat
    18601 agctaatgtt atgcgtttcc aacgcgtatt ccttttcttt ttcatagagt tcactcccat
    18661 gatcagccgg atgattgtca tatgaagata attcgttaag gtcctccttt atattggatt
    18721 ccccaataaa ttcccggttt actctcagcc tttgggacgc tatctccctt tccgatatga
    18781 gcttgtctct caaatagtta atctcatgat ctttcattag attgctcctt caagtttctc
    18841 ttataagagt attccccctt tttatagatt aaaacagatc ctatagaaat gtctggctgt
    18901 taattaatgc tttatccgct gggcagaccg tctccatcca taccctcata tgtcggaatt
    18961 tcttgattta tgacctctaa ttgagtctct agcaggcttt tgaagcgagt tttaaagact
    19021 tggatttgct tcctatattc atcgaattcc ctccgaataa ggattaccct atcgttggcg
    19081 tcctggatta tttttctccc tttgctctct gcctcgtcaa taattaggtc agatcttttc
    19141 tgggaagatt ccaatacatc gctggccgcc ctttgtgcgg taaccagggt ttcttttaaa
    19201 gtattttcca tggtcttgta gttactgatt tggtctgcca gcatacccaa acgttccttc
    19261 aattcgatat tctctttata taatctttca aattcatcga ttatctcgtc caaaaattgg
    19321 tcaacctctt catcgtcata tcctctaaag gatttggaaa attccttgtt atgaatatcc
    19381 atgggagtca atgacattgt aagccctcct ttaatccatt ataatttggt tatatatatc
    19441 tgatgatcaa catcttttca cagcaccatg ggctgggtcc ggttgcatca ctaaggttgc
    19501 aagcctaatt gtatcaattg tttttaaagc aatatattca catcgtacac ttaccttaaa
    19561 agcgagccaa gcaaagtcgt tataacagac aatgtaatgt atgccaacag gggcgaaaag
    19621 tctatgggga gccctgaggt atttagcttg ctggtgaggg cacggaaagg tgatacaata
    19681 ggttcaacaa accgggccaa aaaggtcctc accggatgca tagggtcaag gacccatgat
    19741 agtaatgaat tgaggataat agcccaggat aaaatgttaa gaaaccaaat aacgctgtta
    19801 actaaatatc tgaaaacttg gtcatgccca aacaaattgg cgcctcctaa tcgtctattt
    19861 ttttgttgga tagggtgaaa aaactcttcc cctttaattc ttcgggaata ttccccacaa
    19921 tatcaacatt gctgggagcc aatacaaaga tacccctaga taccttctgt atagtcccgt
    19981 ccagggaata aacggcaccg ctcataaaat ctaaaaccct ttgggccaaa tcagcctcga
    20041 gtgaatccag attgactaca ataggctttc ggctttttag attatcaatg atattttggg
    20101 tatcgtcaaa ggacagaggt tggtatacca caaccttcac ataggtggat gtatgaatat
    20161 tgaccacctt tcccttttta ttccgagatt taaaattggg ttcaatgagc ctgtcattat
    20221 ccctgtttaa atcgtcttct ataacggttt cttcaacctc ctcgagtata ttctcctcca
    20281 gtccgatgat gtttaagagt ttattgaaaa ttttattgga catgcttatc ctccttcaga
    20341 aatctaatgt aaagtcatgt atcctgaaaa atggcccggc caatacgcac catattagcg
    20401 ccctcttcta cagcgatcat ataatcattt gtcattccca tagatagata ttgcatatca
    20461 atatggctta tatcactctc tcggagggca tcaaagtgaa accttaaacg cctgaaacat
    20521 gttcttatag cctccttatc gggggtatta ggccctatag ccatcaaacc tttaaccttt
    20581 atgtatggat agttagccaa cgattcaata aaaggatgca gatcctcctc atagacacct
    20641 gatttggtgt tttccctcgg caaatttacc tgtaccaaga cctgcaccgg tctattattt
    20701 tgttttgccc tcttgttgat ctccttcgca agggttgtcc tatccaagga gtgtattagc
    20761 ctcactttat ctataatata tttaaccttg ttggtttgta aagggccaat aaaatgccaa
    20821 tcaatatttt tttggacact ttcgtactta cccaatattt cctgtactct gctctcaccg
    20881 aattgcgtga ttcctgcctc tgccgcttca tctatcctat agatgggtac atttttactt
    20941 acggcaataa cggatatatc ctcgggcttt ctattgaccc ttaatgctgc ctcatatatc
    21001 ccatccctta ccagttgaat tttttcttca atggtcatct aaaaaatcac ccccattatc
    21061 acaaagcata gtccggcgat gcctcatatg ttacgcataa tactctgttt aaacactaga
    21121 tcaaataaat catctgattt tgcagtgaaa gacctttcta tatatgttac gaaaaagaca
    21181 tttcattata aaatcggcaa aaatatccga ttttacatta aaataatgta tttttcgtaa
    21241 catacgaatt agggataatg ttttacgaca tctgtctacc aatatagaat gtttgtcgaa
    21301 aacatatagt aatgaaagac ccttctaata gagcatacag ctaatttact aatattttat
    21361 cgttaaggtc aagaatatcc gtttcattca tccttgatat tatagaccgt tcaccgtccg
    21421 tggcctgtac ttggatttcc agatactgta tctttccatc tcggagaaca tttatgcccg
    21481 ttttatccct cttttttacg atggccttat ttgggatcat aagacctttg atctgtgtac
    21541 caatctctaa atgaacattt cgggctccca atatatcaac aacatcatca gtcatttcca
    21601 gggctatcaa tacagaatcc gctaaatatt ccgtcttata cactttcccc atgagttcat
    21661 gatcggcaga gcccggcaat gtaattttta ccgaatcatt tcgctcgtat agtacattct
    21721 tattgggtat aagggaaatg atataccact tgtccggtat aacaacctta aaaaggggaa
    21781 caccggctat aatctcatta ttattataat ccgggacctg gtgttcaaga aaagacatat
    21841 atccctcaag ggtaaggaga tccacggtgc caggtgttag ttgggtctcc aagccgtcca
    21901 atttaaaaga aataaacccg gtttcaggtg atataatatc tactctccac tcgtctagga
    21961 tttctttcat atcattttcc tgggttaaca gatgatccag gtaattattt gataattccc
    22021 gcttgtccaa aatggccttt ctttcgctta gaagcccctt aagctgatat tcatgccttc
    22081 ccatttctga tattgctcca ctaacggacc catattgaat agcatgcaga atctcctcta
    22141 tgtcttcctg gactctatca tagtcggtat ctatcaaatc ttgtatcaga ttcttatttt
    22201 gatagtccga tatcttttgt ctgattttgt acaggtcctc cattatcatt ttattatagt
    22261 tatccttaaa aagaatggct ataggatcat tttcttctac catctcaccg tccgaaatac
    22321 aaaagtctac ctttccatag gccggtgcaa agtatggttt ctcatcccta acgatgatgc
    22381 cttggcattt atcccaagtt gtaatctcac catactgaat aacatcatat aggggacgtt
    22441 ttaatgtaag gctgatgagg taggataggc ccagcaaggt aattatcaag aaaacaagga
    22501 acctaagctt cattcttctg ttcccaataa tgatacccct agtcatatcc tacccacctt
    22561 cttaatataa cacttaaata tttctatatg aaaataaaaa atcctcccaa tttccaaata
    22621 aagttcgcag ggaatggatt attgtaaagt actatttgac agattgtaac gaagaattgc
    22681 ttctatcttg ggcaaatgga ttttgtgatc ttgacatgac tgccatttgc atcgaactga
    22741 attttgctgc acaaggcatt gacaagcata agacccctgc ctctttcgga tagttttagg
    22801 atatcatctc caagatagcc ctggcatatt cgtttgtagt caaacccctg accttcatcc
    22861 ttaatgaaaa tatctaaggt agtaggattt acctctttaa tagccacgtt tacctttttg
    22921 ttacaaaggc ctttgttccc atgaaatatt ccattggaaa taagttcatt taagataagc
    22981 cggaattcat aatattcttc ttcttttaaa tggttccact ccataatgca agccatcatt
    23041 tcacttatta aatccctaac agcggggggg ctgtcgccta ctatttgctt tttaaagagc
    23101 agcctcatcg attctacctc catagaccat atagtgtgca aaactcaact ataatatctc
    23161 taccactttt atgttcatgt aaaacagcaa atgcgaaaat aggacgagga tatataagtc
    23221 caattcaatc atatggtcct tttaatatag gatctgaatc gttctaacag ccgtcgctcc
    23281 atccgagaaa tatacatctg cgacactcca atttcctcgg caatttcttt ttggctacgt
    23341 tcattataaa agcgctcata taaaaccttt ttctccgtat cccctaaacc cgccattatc
    23401 ttttcaatga tatccctgtc ttccacccgt gaataatttt tctcctccct cccaatcata
    23461 gccataagat ccgtttcgtt atcatcgtca atatagtaat caagggaagc ggtattggtc
    23521 aagtagctgg attccatcaa ctgtaatacc tcttccggag tgacttttaa atattcacca
    23581 atctcttcta tttttgggct acgccccagt tggttggata ggtatgttcg ggcgtcttca
    23641 accttcttta tcatttcgcc ctctcttctg ggtaagcgta ttatcctgct cttatcacga
    23701 aaatggtttt ttatctcccc aattatggtg ggggtcgcaa agctggaaaa cttataaccc
    23761 ctatttatat cgaacctttc aagggctttt attaacgcca atgaagcaac ttgaaacaga
    23821 tcgtcgtatt cgatccccct atttacaaat ttttttgcaa taatctccgc tatatagaga
    23881 taatgattta ccaattcgtt tctcagtcca atatctcggg tctcggaata ttgccggagc
    23941 aatgtctcac cattttcagt tttaatcgta ttgtttatct gtccacacat tgatcaatta
    24001 gacccccaga tctttatgca taaatatagc tcgtatcgta ccattatcaa gatcaaattt
    24061 tacactatcc atcatagagc tcaggatata tttaccaagc tgggcttgac ctaaatcatg
    24121 ggtcgagata ttgctgcttt cccaatcccc ttctccaaaa atttcgatgc ctatcacctt
    24181 cggatccaga ataaagctaa tcgacaactt ttcgggcctc gtcaattggt ttattatcat
    24241 tattatcgcc tctgcaactg ccatctttat atcttcaatt tgatcgacat caaaacctat
    24301 tctagtggcc acagctgcac tggatagtct ggctatcatg acgtattccg gcttggcggg
    24361 aatggccaaa gttattaaat cactgctcac gatttcccac ctctatagta aatattttat
    24421 ccaaacctgt aattacaaat atcttgtata tatatggttt taaattccta atctttattt
    24481 ccccattgta ttcttttacc cggcgcaaag cgctgattaa tacgcctagc ccggtgctgt
    24541 caatgtagtt taaactgtcg caatccagga taaaatcacc cggattttgt tggatcagtt
    24601 gatgcaactt ttccttaaat tcaggcgcat tataaatatc tatttcccca tctaaggata
    24661 tgtaccacat tttggtatca gaatcgaatc tttcgtccaa tatcatcgta atgctcctct
    24721 catttaatat ttgctaatat tcgcggttgg tataaaaagt aatcaccgtt gatccgcaat
    24781 tcaataacct atatcatata ccaactcgtt ttctggtcaa taatgatatt ataacataaa
    24841 actaaaaaaa atgtttatta atgtatacag ttgactgtat tggggcaata catttaaaag
    24901 ccacaatcat cataaattac tatgttcaaa ctttggagtg acccgccgga taatcccaag
    24961 aaaacaattt ctgatttggg gtataataaa tttgcattat ttcacttgac agtgatatta
    25021 aaatattgta tagtataatt cgtcgcccga aaacaatata tcggggtgta gcgcagtttg
    25081 gaagcgcacg tgctttggga gcatggggcc gggggttcaa atccctccac cccgaccata
    25141 tataaatttt ccttatgtgg ccccttggtc aagcggttaa gacaccgccc tttcacggcg
    25201 gtatcagggg ttcgagtccc ctaggggtca ccatacataa gggcctttag ctcagttggt
    25261 tagagcaacc ggctcataac cggtcggtcc ggggttcgag tccctgaagg cccaccattt
    25321 ttttacaata tatatctggc ccggtagttc agttggttag aatgccagcc tgtcacgctg
    25381 gaggtcgagg gttcgagccc cttccgggtc gccatttttt tgcctcggta gctcagtcgg
    25441 tagagcagca gactgaaaat ctgcgtgtcg gtggttcgat tccgccccga ggcaccatct
    25501 atttttccaa ctattatgcg aaagtggcgg aattggcaga cgcgctagat tcaggttcta
    25561 gtgggcaata cgctcatggg ggttcgactc ccccctttcg caccaattct tttccggtat
    25621 agacattcta agaacatcat aataatcgtc cgccaatata tagataagca tctagatctt
    25681 cttatagaaa aacgttttta cggattcaaa gccatatttt tgaggaaaaa ttatatgctc
    25741 cgtactgcct acaaacagga ctccacccac ttccagagac tccctaaatt tgggatatag
    25801 ttgattcttt gcctcctcag taaaatagat cataacatta cgacatataa ttaaatggca
    25861 gtcagtggga tatggatcca tcaataggtt gtgttgtcta aattcaattt gctttttaat
    25921 tttttcatct attatataaa gcccatcttc catggtaaag tatttcttta cataggattt
    25981 gggcaaagat tgcagacttt tttctagata gactccttcc cttgccttcc ttaggacttc
    26041 cctgtcaata tccgtggcta taatttttac acggtttagg gataaataat cctgtagcag
    26101 catagctaat gaataaggct cttcccccgt agagcaagcg gcactccata tttttagctg
    26161 attattcttt ttcaacaaca tcggaataat atcctttctc aaggtttccc attgattggc
    26221 gtttctataa aactctgaaa cattaatggt tatgtaatca ataaagttcc ctaacatagt
    26281 cttgtccgca ttcaaagccc tataataatc ctcatagtgt acgtagttat gcctgctgat
    26341 caacgaatca attcttcgtt tcatctgacg ttctttgtaa caagatagat cgatctgagt
    26401 caatttaaag aaagctttgg taaaatcccc ataactccag tgcatttgca ttacccctta
    26461 acaagattct gtttgtatag tctatcacga atatagataa aaaaaaagtc gtgaaatgtc
    26521 acgacttcat gtaaaggggg tctcaatgtg tttgtgaggt caattatatt attagcttaa
    26581 taactttttt tatacaaaaa gaaaggcaga atatctgcct actaaaaact tatgataccc
    26641 tgggcataaa caagactaat taaaatcctc cgggaaaaat tcgcccaacg aaaccgtcat
    26701 atcttctttt tgaaattgat cttcttttgc tttcggtctg gtcgattccg gctttaccct
    26761 ctcatcaggt aatagcttcc ttatgctcag gctgatcctt ctgctgtcag aattagcatc
    26821 caagactttt acatcgattt gctgaccgat ggacaactca tcttctacct tctggaccct
    26881 tctgttagct acctgtgaaa tatgaactag cccgtccaca ccgggttcca gttcgataaa
    26941 tgcaccaaag gaagtgatgc gcactacctt cccatttaca actgaacccg caggatattt
    27001 ttcttcaaca ttgtcccagg gatgtggcaa agtctgctta tatcccagtg aaattctctc
    27061 tttctcacga tcagcagcca atactataac ctcgacattc tcctcctcgc tgaggacctc
    27121 cctgggatta tttacatggc cccaggatag atcagagata tggataagac cgtcgacacc
    27181 gccgatatca acaaacgcac cgaaattcgt caaacgcttt actacgccct gaattcgttg
    27241 gccctcttca attgaagccc atagcttctg tcgcttttct tcttcctccg cctctagtat
    27301 aactctttgt gaggctacca cccttctacg ccttctctcc agctcaatta tcttgagccg
    27361 tagcgtctgt cctacaaata tgctaagatc ctcgatatac ctgttggata gttgggaggc
    27421 gggtacaaat gccctaattt ctccaatcct tgctatgact ccgcccttta cggattctgt
    27481 gcatacacct tcaaattctt ctcccgtttc taacccctgt tctatggctt tccatgcctt
    27541 ttttctttca acggatttac gcgacagtat tacattgcct tcctcatcct tcaccttaag
    27601 gacctctacc tccacttcgt cccctgggct aaagatatca agaggagtct ggtcaccatc
    27661 gagtatgagc tcgtttaggg gtattatacc atcggcttta tatccgatat tgacaataac
    27721 ttcggattca ttgacagata agacattgcc tttaattaat tggcctgatc tgagagttac
    27781 cattgtctcc tcaaatcctt ccatagtggt tatttcttcc gaattatcat cttcactatc
    27841 ctcttctgat tcttctgcag aatcagactc ctggccttct atggattctt cttcttcctg
    27901 agaaatatcc tgctcctgta catcaacggg tgattcctcg ttctcatcca cagattcgct
    27961 tattggcgat tcagcggtct caatttcaac cgccgaagaa ttgtcttcct gtgcttttat
    28021 tggttcttca ttaatctctt cagggatcgg tgtttcgttg tcaatgccct ggctatcatt
    28081 ttggacatca ttgatctttt cattgttgtt tccttcgaat acgttcatcc tattaattac
    28141 ctccttgatg acccaatcag atacggagtg cacctggggg tactaccaca ccctatggat
    28201 cttaaacatt gaaatcaata tcattcgctg tttaaacaat aagcgtattt tcacagcatg
    28261 gtttgcgaaa attaaaacta tcttgtgtgg gtttttccac ccataaataa cctacacaat
    28321 cttagcgttt ttctcggtcc tcgcctccct ttggaatgta gtaacacatt aaaccctatc
    28381 ccctatgttc tggtgcaatt atcctcgcag gtgataataa tatcatgccc ataacgatga
    28441 tattcttccg ccgacccggg gcatggttag cgttcgggga tatgaaatga atatacaatc
    28501 actttagccg ttatgttacc atatgcatta ccttcaaggc caaggatcag atacaaaagc
    28561 tatatccaat tcattatcct gctcatataa aaatatatct ctatctatta cttttcgata
    28621 taatagcact aaatccttct tttttgaaaa aataaagaaa aaataataac tttatgggct
    28681 ttatccctgt ttattaaata aggatttcag cgaattctcc atttctgtag atatcaattc
    28741 catcaattcg gtgttagatt tttgagaaaa ataattatcc atatccagaa cttcccctac
    28801 tacaacctcc atgggatgaa aaagcttgta tcttccgatt actcctactg ggatcacctt
    28861 tgcctttgat ctgtgagcaa tggacgcagc accgagtgag aagtgtttaa cctccccttg
    28921 tttaccccgt gtcccctcgg ggaagatccc gaatacatta ccacccttta agaccttaag
    28981 tgaatttttt attgcggcaa gatcagcagt gcccctctta acgggaaagg cacccacatt
    29041 ttttagtaca aatcttagga tgggattttg aaagagttcg gattttgcca taaagtagat
    29101 tctccgtggg agcatacacg ctatgagaat aggatctaac atggatatat ggttggaata
    29161 aaggacgact ttcccagtct tcgggaagtt gtccaatcct atagtttttg ggcggtacaa
    29221 aagaaacagg ataggacgaa gtaagcccct tattatctta taaaacattc atactccccc
    29281 cttacccctt tcggcaatat ctttctgtat gtcctagcat ttcggctaat acctccccta
    29341 tagatttacg cgttgtatct atggttatag cgtccttggg tacttccaag ggcgcaaaac
    29401 tacggttttc atcaacgcta tcccttgccc ttatatcctc tttcaccgct tcgatgcttt
    29461 cctcatatcc ccttaaacgc atttcctccc atcttctccg ggcgcgctct tctaaggaag
    29521 cggttaaaaa aaattttaaa gttgcatccg gtaacacata agtgcctata tccctgccgt
    29581 ccattaccaa tgaatttgat tttgctatat cgcgctgtat cttcgccaat cttattcgta
    29641 cctcgggcaa aacggctact cgagaggcac ccattgatac ctcgggacat cttattttat
    29701 cagatacatc aatcccgtct agcacaatac cttggttgcc attattaaac tcaatttcta
    29761 tctctgttga agcgagggca gaaactatcc cttgggtatt gttaaaacca atatctgcgt
    29821 tgagcatttt gtatgcaatc gctctataca ttgcgcctgt atcaaggtag attataccca
    29881 gcttctttga aagtgccttg gcaattgtgc tcttaccggc ccctgccgga ccatcaatcg
    29941 ccactgtaaa cggttgatcc gatatcgttt tgctaacgtt gtctcggctc agatccggcc
    30001 ttagggattt cgcttttttt aagtagacat gctctatagt tcgtgataga ggtgattgta
    30061 tatgcatcat aatgcgaata catttttcca aactgccttc gacatgcatt tcttggcaac
    30121 aaattaatgg tatatctacc attcccaatt cccgtgctgc tactgcggga taaattgaat
    30181 ccagatccct tgtgcctgta aaaatgatgt tgacgatttc actatattca atattgtttc
    30241 ttttaataat ttcctttagt agcatttcag tgttgtctaa aatctgtggg gatgtgttat
    30301 catctgctgt aatagctccc cttattgcat aaaaaaacaa ttacttttcc ccctcatata
    30361 gctctgtgac caagaccaac agctacttca ttgagatgaa gcaaccctat ccccaagaac
    30421 aaggccacac tcacatggtc atggtatctt gctacggcta ttttacctcc cacactcagt
    30481 cccatggctt tgggcatgat ttgagttatt gcctccctag tagcgccggc tatggcacct
    30541 tcttcagcgt gggaactccc tattacgcct tcccgcttgg aagcaactac tgccctttca
    30601 attatcttat ttactgaggt gatgaaatct ccgccgtaat caatggccac cgttcttata
    30661 cctgttttga gatactcttc tttatggaaa ttctcttcag ccctattctt tgttaaactc
    30721 aactgaattg caattctggc aacctcacga ctccctagca aatccatatc atatctcctt
    30781 tatgataatg tatgtacata atcattataa aacagggctc gggcttgtac aagtagtttt
    30841 ttatttaatt ggggataatc tgtacagttc tccgtctata ttgagttttt gggtatatcc
    30901 ttcgggatgg gtttgagtgg gctccgaatt gatcacaatc tggccgggag ttcggatttc
    30961 cctggcatct atttccaaca tatccttgga taataaatta acgaataggg gatgttgatt
    31021 ttggacggaa tcgattattc tcccatttac cctgattaat acttcgtccc cctcaccaat
    31081 tgctgtctca atatgaatta aaacctgctg gtattcagag cgacttatgg gaccgacacc
    31141 gcttcccata gaatataagg ggttatgggc tccgtaaaag gcactataat agtggtagac
    31201 ctgtaaaacc aataatacaa tagcgagggc taagcccagc cgagacaggt gtgtttctat
    31261 tctttcagta atgatatgaa tggtccctat gtttggatgt ttttttcttg ttctcataat
    31321 ctcatctgta aaacctttca gagtagaaaa aaataaatcg ggaactataa tatat
//
LOCUS       DASRNX010000039         3968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_432797,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000039 DASRNX010000000
VERSION     DASRNX010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432797"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFD89_01555"
     CDS             <1..280
                     /locus_tag="VFD89_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56905.1"
                     /translation="DKITLEIALGKIGDIKDAVKAKTDQLQRYSADQEGSLVLSGLGN
                     LFSEQMENLGDIVQGNVSDKVEISSKIKYTYIDMAVKYQKYIEDITNR"
     gene            294..641
                     /locus_tag="VFD89_01560"
     CDS             294..641
                     /locus_tag="VFD89_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HZJ56906.1"
                     /translation="MKIATIKELLEAELIVGEEFLEHEVVSACGSDLMSDVLAFVKEQ
                     TVLLTGLNNPQVVRTAEMLDVSCIVFVRGKRPAQDIIDMALARDIVLLSTGNTLFEAC
                     GKLYQGGLRGDMK"
     gene            638..1078
                     /locus_tag="VFD89_01565"
     CDS             638..1078
                     /locus_tag="VFD89_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ56907.1"
                     /translation="MKMESNEYIGMVHSYQIESGNFTKAGEAASGIKKLLRQLGVDAK
                     IIRRVSVAAYEAEMNMVIHSTGGVIEIEVNPKGITFKAVDEGPGIEDISRAMEEGFST
                     AKEWARELGFGAGMGLPNIKRCCDDFHIQSQIGQGTCLEARFSL"
     gene            1394..2758
                     /locus_tag="VFD89_01570"
     CDS             1394..2758
                     /locus_tag="VFD89_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HZJ56908.1"
                     /translation="MQEYFHSVTLDEDKCMGCTNCIKRCPTEAIRVRDGKAYIIAERC
                     IDCGECIRVCPYHAKIAVTDSLDSIDKHKYKIALPAPTLYGQFEKLTSIDSVLHGLKA
                     LGFDDVFEVARGADYVTEFTKYQIEHSDPKWPIISSACPAVVRLVQVGFPELINNLAD
                     VQSPMEIAAQIAKDEFSREHGVDEDEIGAFFITPCAAKATSIRNPLGAEKSSVDGAIS
                     ILDIYGPLSLVMNREGILNDSVSLQKSTLKGVGWANSGGEGTSLGIDSYLAVNGIHNV
                     MRVLEEIENGKLSDLDFFEGLACVGGCVGGPLVFENNFVAQNRLKNIISRMEPPAPIT
                     HEELERAIDRYNWILNKEILPKPIMILDEDISSAIKKMGELERIYADLPGLDCGSCGS
                     PSCRTLAEDIVRGYATEFDCVIKLREKVKNLAREVIELSDNLPTGRERRGTGGLTHED
                     KGSD"
     gene            2736..3080
                     /locus_tag="VFD89_01575"
     CDS             2736..3080
                     /locus_tag="VFD89_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56909.1"
                     /translation="MKIKDLIKGMPHTVLVGEEFLNRPIKSGYCGDLLSRVMARGPRN
                     GVWITVQTHVNIIAIASLMEISCIIIPEDISVDPNTIEKADEEEIVIISSPLTSFELS
                     GRLYGMGIGNRE"
     gene            3077..3778
                     /locus_tag="VFD89_01580"
     CDS             3077..3778
                     /locus_tag="VFD89_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZJ56910.1"
                     /translation="MRMAVDLHIHSALSPCAHDHMTPNNIVNMAMLKKLDAISITDHN
                     SCDNVWAVIEAAGSKLIVVPGMEVQTREEVHLLCYFCGLDKLMDFGSLIKRRLPDISN
                     APDLFGNQFIMDERDRIVGVREEMLIASVDLSIDEILDEVSSRDGVVIPAHIDKPSYS
                     VISQLGFIPQELKGGMLEISRGNNGFLAGFPPERFFYSSDSHELGQILERESFLRVWE
                     PSIPGILDALGNPPK"
BASE COUNT         1202 a          727 c         1034 g         1005 t
ORIGIN      
        1 ggataaaatt actttggaga tcgccctggg gaaaataggg gatattaaag acgcagtaaa
       61 ggctaaaacg gatcagttgc aacgatattc cgccgatcag gaaggaagcc tggtattatc
      121 aggtctggga aatctctttt ccgagcagat ggaaaatttg ggggatatag tgcagggaaa
      181 tgtatcggat aaagtagaaa tttcctccaa gataaagtat acttatatcg atatggcggt
      241 taagtatcaa aagtatatag aggatattac caacaggtag ggagagatag tatttgaaga
      301 tagcaaccat aaaggaactg ctggaggcgg aattgattgt cggggaagaa tttttagagc
      361 atgaggtcgt atctgcatgc ggatcggatt tgatgagcga tgtattggca ttcgtcaagg
      421 agcaaacggt cttattgacc ggacttaata acccccaagt ggtccgaact gccgagatgc
      481 tggatgtatc ttgcatcgtg tttgtgaggg gaaaaaggcc ggcacaagat atcatagaca
      541 tggccttggc gcgtgatata gtactgttat ccaccggaaa caccttgttt gaggcatgcg
      601 gtaaactcta tcagggggga ctcagaggag atatgaaatg aagatggaat ccaatgaata
      661 tattggcatg gtccatagct accaaataga atcgggtaat tttaccaaag ccggtgaggc
      721 agccagcggg attaaaaagc tcctaaggca attaggcgtt gatgccaaga taattagaag
      781 ggtctccgtc gccgcatacg aggcggaaat gaatatggtt attcactcaa caggcggggt
      841 aattgaaata gaggtgaacc caaaagggat cacattcaag gccgtggatg aggggccggg
      901 tatagaggat ataagccggg ctatggaaga gggattttcc accgctaaag agtgggccag
      961 ggaattgggc tttggagcgg gcatgggatt gcccaatata aaaaggtgct gcgacgattt
     1021 tcacattcaa tcccagatag gacagggcac ttgcctagaa gctaggttct cattatagga
     1081 tgggcattac cctttcaagg ccttatccgt gactaatcct acgatataat ttgacctata
     1141 aattgctcgt taaaacaccg gaagcaattg tattggcaat tgcaacacac aggtttcaac
     1201 gaggcgggtg atttgtaccc accgcctgtt ttactaatgg gaactcgccg cttatagaag
     1261 caggagacat cctaccgact cgttatattt gtcacatagc aagataatga agtgatacaa
     1321 tacatcaatg tagaaaacag cgacgaatta tgaaaagacg gccctgtcta tgatgtaaag
     1381 gaaggtggtc tatatgcagg agtattttca ctccgtaacc ttggacgaag acaagtgtat
     1441 gggatgcacc aattgtatta aaagatgccc cacggaggca atacgcgtca gggacggtaa
     1501 agcttatatt attgctgaac gttgtataga ttgtggggaa tgcatccggg tctgtcccta
     1561 tcatgccaag attgccgtca ccgattcttt ggattcaata gataaacata agtataagat
     1621 agcactcccc gcacccactt tatatggcca gtttgaaaag cttaccagta tagacagcgt
     1681 attacacggt cttaaggcct tggggtttga tgatgttttc gaagttgcca ggggtgccga
     1741 ttacgttacc gaatttacaa agtatcagat agagcattcc gatcctaaat ggcccattat
     1801 ttcttctgct tgtcccgctg tagtaaggtt ggtgcaagtt ggttttcctg aactgattaa
     1861 caacctggcc gatgtacaat cccccatgga gattgcggca caaatcgcca aggacgaatt
     1921 cagtcgtgaa catggggtgg atgaggatga gataggcgcc ttttttatta ccccatgtgc
     1981 agcaaaggcg accagcatca gaaacccgtt gggggcagag aagtccagcg tggatggggc
     2041 tatatccatt ctggatatat atggtcctct atcattggta atgaacaggg aaggtatatt
     2101 aaatgattcg gtcagcctcc agaaatctac cctaaagggc gtaggctggg caaactcagg
     2161 gggagaagga acctccttgg gtatagacag ctatctggcg gtaaacggga tccataatgt
     2221 tatgagggtg ctggaagaga ttgagaacgg caaactatct gatttggatt tttttgaagg
     2281 cttagcctgt gtaggcgggt gtgtaggcgg ccctttggtt tttgagaaca actttgttgc
     2341 ccaaaacaga cttaaaaata tcatcagcag gatggaaccc cctgcgccta ttacccatga
     2401 ggaattggaa agggccattg atagatataa ttggatacta aataaggaga tattgccaaa
     2461 acccatcatg atattggatg aggatatttc atcggccata aagaagatgg gagaattaga
     2521 gcgtatatat gccgatcttc cgggtttgga ctgcggctct tgcggatcgc ccagttgcag
     2581 aactttggcg gaggatatag taaggggata tgcaactgaa tttgattgcg ttatcaagct
     2641 tagggaaaag gtaaaaaatc tggccagaga agttatagaa ctgtcggata atttgcctac
     2701 gggaagggaa agaagaggga caggaggatt gacacatgaa gataaaggat ctgattaagg
     2761 ggatgcccca taccgtgtta gtgggggaag aatttttgaa caggcccatc aaatccggat
     2821 attgcggtga tctgctgagc agggtaatgg cccgcggccc aagaaatggg gtatggataa
     2881 cggttcaaac ccatgtcaat attattgcga ttgcatccct aatggaaatt tcgtgtatta
     2941 tcatacccga agatatcagt gtggacccaa atacaataga aaaggctgat gaagaggaga
     3001 ttgttataat ttcctcccca ctgacgtcct ttgaattatc cggaaggtta tacgggatgg
     3061 gaataggcaa ccgggaatga gaatggcggt cgacctgcat atacactcgg ccctctcccc
     3121 ctgcgcccat gatcatatga ccccaaataa catcgtaaat atggcgatgc tcaagaaatt
     3181 agatgctata tccattacgg atcataatag ctgcgataat gtatgggccg taatcgaggc
     3241 ggcaggatcc aaattgatag tcgtacccgg tatggaggta cagaccaggg aggaagtcca
     3301 tcttttatgc tatttttgtg ggttggacaa gctgatggat tttggatccc taataaagag
     3361 gcgattgccg gatatatcca atgcacctga tttatttggc aatcaattca ttatggacga
     3421 aagggatagg attgttggtg taagggagga gatgctaata gcatccgtcg acctatccat
     3481 agatgaaata ttggatgagg tctctagcag ggacggtgtg gttatccctg cccatatcga
     3541 taaaccctca tacagtgtca tttcacaatt aggatttata ccccaagagc taaaaggtgg
     3601 aatgctggaa ataagcaggg gtaacaatgg ctttttggcc ggattcccac cggaaagatt
     3661 tttctattct tcagattcac atgaattggg tcaaatattg gaacgggaga gctttctaag
     3721 ggtatgggaa ccgtcaatcc caggcatcct tgatgcacta ggaaatccac caaaatgatt
     3781 atcatttgga catagggcct tgataaaact taaacccgga ctagagtccg gtcaattgat
     3841 aattaaataa cgaaaggttc tgaatattgt atcattctat agcagagggg atgctgaagg
     3901 acaaaattat aaaagaaata aaacacgaga tataatagat aaagtcgaag tatgtaaatc
     3961 gttgaaaa
//
LOCUS       DASRNX010000040         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_697606,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000040 DASRNX010000000
VERSION     DASRNX010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697606"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..222
                     /locus_tag="VFD89_01585"
     CDS             46..222
                     /locus_tag="VFD89_01585"
                     /inference="COORDINATES: protein motif:HMM:NF020945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0E family sporulation regulatory
                     protein-aspartic acid phosphatase"
                     /protein_id="HZJ56911.1"
                     /translation="MTLEELEEELEEKRELLADILRKGDLNLVDGEVLALSRSLDKLI
                     ATYLGMVATNASKP"
     gene            complement(438..944)
                     /locus_tag="VFD89_01590"
     CDS             complement(438..944)
                     /locus_tag="VFD89_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56912.1"
                     /translation="MKDTVIVFVGIGVLILLPLIFFGVRSIAKSGKEKDYRRLLLRLL
                     GTVLACAVAVVLLFNIYSFTLGYQLPLVAEGFLKKEGYAQLKEQGWDMDKFLVYFSEN
                     IYENDDGTKTIYVQFQGEEDSNYSFMNMKMVGNRWQVLSHNIITGDYDDYPEISKRFY
                     LIDGKLPK"
     gene            complement(959..1681)
                     /locus_tag="VFD89_01595"
     CDS             complement(959..1681)
                     /locus_tag="VFD89_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZJ56913.1"
                     /translation="MIEVVNLHKYFGKLEVLKGVNTSIEKQEMVCVIGASGSGKSTFL
                     RCLNLLEEPTDGEIIIDGIPLVEQRKDINHLRQKVGMVFQQFNLFPHMTVMENITLAP
                     IKLKGATKAEARKKAKDLLYKVGLPDKDNEYPNRLSGGQKQRVAIARALAMDPEVMLF
                     DEPTSALDPEMVGEVLNVMKQLAKEGMTMVVVTHEMGFAKEVGDRIMFMDEGIIFEEG
                     PPQDLFNNPKKERTREFLGKIL"
     gene            complement(1674..2357)
                     /locus_tag="VFD89_01600"
     CDS             complement(1674..2357)
                     /locus_tag="VFD89_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZJ56914.1"
                     /translation="MDSIIKSFSDAIYYNLIREQRYMQFIQGLGISLQLTLYAAVLGT
                     LIGLFLALAKLGRIKWLESLATLYVDIIRGTPAVVQLVLIYYAILGSSSLPKIVVASV
                     AFGINSGAYVCELIRAGILAVDRGQMEAARSLGFSYSHSMLYIIIPQAIKNILPALAN
                     EFIVLLKETAIAGYIALDDLTRAGNIVRGRTFDAYTPFIVVALIYLYITTILTLMLNK
                     LEGRLRESD"
     gene            complement(2729..3631)
                     /locus_tag="VFD89_01605"
     CDS             complement(2729..3631)
                     /locus_tag="VFD89_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HZJ56915.1"
                     /translation="MKNINKFFLVLVILLFTISVIGCSAGGKVDDASKGNGTSDSDNS
                     GQENAGDQDTTDGDDSNDGDTSDEVGIIDKIKDNNKLIWGTNAAFAPFEMREGNEVIG
                     IDAEIAQKIADKLGVELVVEDMEFDSLIAALQSGKIDFIAAGFTIKPDRQEKVLFTDT
                     YFKAVQAVMIQDGNPDIKVYEDLEGKKIGVQIDTTGFYEADDIKDAEVIQFNNGIEAV
                     LDLGNGNIDAVIIDNLPAQILVNSNPDLVILDFDDKPFEDEEYAMAVRKEDTELQEVI
                     NEVLKELQDSGEIGELVNKYSIGE"
BASE COUNT          967 a          894 c          704 g         1083 t
ORIGIN      
        1 atatattagt ttatggcata atgctttgtg gggggtgagg ttaatatgac acttgaggaa
       61 ctggaggaag agcttgaaga aaagcgagag cttctggcag atatccttcg caaaggggat
      121 ttgaacttgg tagatggtga ggttctcgct ttaagtagga gcctggataa gcttattgca
      181 acttatctcg gtatggtggc cacaaatgct tctaaaccat aaaggttcgg gataaattcc
      241 cgttccatat atggcaagga cagggatgat tccctgtcct tgccatagca ccataagaaa
      301 atatccttca ctttcgcaaa tgatgggttt tcattaatta aaaagtgatg ggcaaaaatc
      361 ccccgcctcg ttgaaacctg tgtgatgcaa ttgccaatgc aatcgcttcc gatgttttaa
      421 tcttcgcaaa caggctttta cttgggcagt ttaccatcta taagataaaa tctcttgctg
      481 atttcggggt aatcgtcata atccccggtg attatattat gggataggac ttgccatctg
      541 ttcccaacca tcttcatatt cataaaagaa taattgctgt cctcttctcc ttgaaactgg
      601 acgtatatgg tcttggtccc atcatcattt tcgtagatgt tttcactaaa gtatactaag
      661 aatttatcca tgtcccagcc ctgttctttg agctgggcgt atccttcctt tttcaaaaag
      721 ccttcagcca ccaagggtaa ttgatagccg agagtaaagc tatagatatt aaataacagg
      781 acaaccgcta cggcacaagc caggaccgtc cccagtaacc tgagtaaaag acgcctataa
      841 tccttctcct tcccggattt tgctatgctc ctaaccccaa aaaagataag aggcaacaag
      901 attaatacgc ctattccgac aaaaacaata acagtatcct tcatgtatat ctacccccct
      961 acagaatctt acctaagaat tctctggttc tctctttttt tggattattg aacaaatcct
     1021 gcggcggacc ttcctcgaag ataatcccct cgtccataaa cattatcctg tcccccactt
     1081 ccttggcaaa ccccatctca tgggtgacca ccaccatggt cataccctcc ttagccagct
     1141 gcttcatgac gttcaatacc tcgcccacca tctccgggtc cagagcggag gtgggttcgt
     1201 caaaaagcat cacctcgggg tccatggcaa gggcccttgc gattgccacc ctttgttttt
     1261 gcccgccgga gagcctgttt ggatattcgt tatccttatc aggaagaccc accttgtata
     1321 gaaggtcctt ggcctttttt cgggcctcag cctttgtcgc ccctttgagt tttataggag
     1381 caagggttat attctccatg acggtcatat ggggaaacag gttaaattgc tgaaacacca
     1441 taccgacctt ctggcggaga tgattaatat cttttctttg ctcaactaag ggtatcccgt
     1501 caatgattat ttccccgtca gtcggttcct ctaggaggtt caaacagcgc aaaaaggtgc
     1561 ttttcccgga gccgctggct ccaataacgc agaccatctc ctgcttttct atggaggtat
     1621 taaccccttt taaaacttcc agttttccga agtatttatg caaatttact acttcaatca
     1681 ctttcacgca gcctcccctc aagcttattg agcatcaatg tcaatatggt agttatatat
     1741 agatatatga gtgcgaccac tataaagggt gtataggcat caaaggtcct gcccctgact
     1801 atattccctg ccctggtcaa gtcgtctagg gctatatatc ccgcaatggc ggtctccttt
     1861 aacaatacga tgaactcgtt ggccaaggcc ggtaatatat tctttatagc ctggggtata
     1921 ataatataaa gcatggaatg ggaataggaa aaccccaagg atctggcggc ctccatctgt
     1981 cccctgtcca cggctaagat gcccgcccga atgagctcac aaacatacgc cccgctgttg
     2041 attccaaagg caaccgaggc taccactatc tttggaaggc tgctgctgcc caatatggca
     2101 taatagatga gcaccagctg taccaccgcc ggagtgcccc ttattatgtc tacatagagg
     2161 gttgccaggc tttccaacca ttttatcctc cccagcttgg ccagagccaa aaacagaccg
     2221 attaaggtgc ctaaaacagc cgcatacagg gtcaattgga gggatatacc aagcccttgt
     2281 atgaactgca tatagcgctg ttcccttatt agattataat atattgcatc agaaaaagat
     2341 ttaatgatcg aatccattta ctaaaaaccc cttttatatt ccgttaggta taggtaaacg
     2401 gccccaactg tagaatttat catctacaga taaacttagt tgatctattg cttaactata
     2461 atcgatattg cccgttacct atttgataat tgcttttgaa acttaccaat tactttgtgg
     2521 gtagcaacca ccaatccaat tttacaatag aatgtataaa tatgcaatag gtgatatatc
     2581 tcaaaatcat cttcagtccg ggtcaatggc cgacaaatcc ttttatcgcc tttggggtgt
     2641 tgggcagagc aaaagccgat atcgaatagt tccaatatcg gctccatgta tatatcaata
     2701 tattcagctg gcccaaggca ttagcctttt attcacctat ggagtatttg tttaccagtt
     2761 ccccgatttc cccgctgtct tgcagttcct ttaatacctc gttgatgacc tcttgtagtt
     2821 cggtatcctc cttgcgaact gccatggcgt actcctcatc ttcaaagggt ttatcatcaa
     2881 agtccaatat cacgagatcc gggttgctgt taactaggat ttgagctggg aggttatcga
     2941 taatcaccgc atcgatgttt ccgttaccca gatccaggac tgcctctatt ccgttgttga
     3001 attgaattac ctcggcatcc ttaatatcgt ctgcttcgta gaaaccggtg gtgtcgatct
     3061 gtaccccaat ctttttgcct tccaaatcct cgtagacctt tatgtccgga tttccatcct
     3121 gaatcataac cgcctgtacg gccttaaaat aagtatccgt aaacaatacc ttttcctgcc
     3181 tatcgggctt tatggtaaag ccggcagcta taaagtcaat tttgccggat tggagagcgg
     3241 ctataagaga atcgaattcc atgtcctcta ctaccaattc cactcctaat ttatcggcta
     3301 tcttttgggc tatctctgca tcgataccga ttacttcatt tccctcacgc atttcaaagg
     3361 gagcaaaagc ggcgttggta ccccaaatta atttgttatt atccttgatc ttatcaatta
     3421 tgccgacttc atcggaggtg tccccatcgt tagaatcatc cccgtcggta gtatcctgat
     3481 cacctgcatt ttcttgccca ctgttatcac tatcggaagt tccgtttcct tttgaggcat
     3541 catcgacctt acctcctgct gaacatccga taacggaaat ggtaaaaagc aagataacta
     3601 aaaccaaaaa gaacttgtta atatttttca tctctattac tcctccat
//
LOCUS       DASRNX010000041         6823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_697738,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000041 DASRNX010000000
VERSION     DASRNX010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697738"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1332)
                     /locus_tag="VFD89_01610"
     CDS             complement(79..1332)
                     /locus_tag="VFD89_01610"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HZJ56916.1"
                     /translation="MDKKTIAFLSLIISVGILILGFSLYKISGQSNVYDMIYWMLLAG
                     LCQSFLVVFNKNRYVSVVFAIIFAVQLTYGTYYSVIIAAGSQTFFVYKEKPQTYRHIF
                     SVPPYKTLFNVANVSISAFTSGTALEFLSKVYELDITKLAFTLLMLIYTLSYALLNTG
                     ILMALMSLLNRDSFFKLWGSLMSWILPNFAAIAPFGYFVALLYNQALGKIYIALLVGP
                     LLLARHSFQLYQNSKEQYYKTVKALTAAIEAKDPYTEGHSRRVEGYASQIAHELRLDN
                     ATIHSIEVAALLHDIGKIGINDSILNKKEVLNSSEFSQIHHHPIIGVKILEDIELPLK
                     SKDIIRYHHERYDGKGYPKGIGGDQVPIEAYILSAADAYDAMTSNRPYRDAMSPTKAV
                     GILIEGSGTQFHPDVVEAFVKVLKR"
     gene            complement(1586..2212)
                     /locus_tag="VFD89_01615"
     CDS             complement(1586..2212)
                     /locus_tag="VFD89_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56917.1"
                     /translation="MSGLTDYEVLINKIPGVISSRFVANQGGEIIELHVLADIYRSPK
                     QLVRDIQSAMLTTYNLKIDHKIISIAQIEDDLIGPREHRFNLHYVKVLSEGPKSQVEV
                     ILLKDGESFLGMAQGGNSLGSRIRLISEAALNAIQNALDIEYVFILGDITPVYVSDKK
                     AYMVTVSHLFELGEELLCGCCIVTKDESETVVKATLDAINRRIVRYFK"
     gene            2567..4801
                     /locus_tag="VFD89_01620"
     CDS             2567..4801
                     /locus_tag="VFD89_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ56918.1"
                     /translation="MLRMIKEIRPFIITILIIIALLFVQAMAELALPDYMSSIVNVGI
                     QQKGIEDVVPNVIRADSLEKIKAFSREDERNLISRSYKLLDRGDLSAEEYEHYVTKYP
                     ALEKENLYILDAADKGEIERLGSFLGRTILIAWGMDQNMPNIEGDTSPFNDLPQGTDP
                     FETLEAVPQEMLDELRAKAEEWFDQMPDSMITQSAISFISTEYEAIGVNIPKAQSNYI
                     LTIGGIMLLIALLVMATSILVGFLSARVSASLGRNLRDGVFRKVASFSNAEFDDFSTA
                     SLITRSTNDIQQVQMFVVMLLRMVFYAPIIGTGGVIRALNTDTSMAWIVAVGVMAVLI
                     LITVLFFIATPRFKRMQKLVDRVNLVMRESLNGMMVIRAFNTQRLEEDKFDKANKDLT
                     ETNLFVSRLMTIMMPAMMLIMNGIMLLIIWVGAHQIEEGTIQVGDMMAFIQYTMQIIM
                     SFLMISMVSIMLPRASVSVQRISEVLDREIIIKDPLSHVELDGGVKGCIEFMDVYFQY
                     PGAEECVLEGITFVAEPGQTTAFIGSTGSGKSTLINLIPRLYDISRGRILLDGIDIRD
                     MRLKDLREEIGYVPQEGILFTGTIESNIKYGKNAVVGEEEVLKSIEIAQAKEFIDERE
                     GGIQSEISQGGTNVSGGQRQRLSIARALAKAPRVLIFDDSFSALDFRTDAALRKAMSH
                     EIVGRTVLIVAQRINTIMKAEKIVVLDEGKIVGQGTHRELLKTCEVYRQIALSQLSEE
                     ELAI"
     gene            4798..6660
                     /locus_tag="VFD89_01625"
     CDS             4798..6660
                     /locus_tag="VFD89_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ56919.1"
                     /translation="MSREKTTRRSGRIGGGFGGRPGSFEKPKNFKETFSKLLRYLKPY
                     GLQLVVVVIFAVGSAAFSIVGPKILGRATANIFEGLIAKISGVPGGGIDFRYIGNIIL
                     ILIGLYLLSALFSLVQGFMVTGIAQKISYNLRKEISQKVNRLSLKYFDTMTHGEVLSR
                     VTNDVDTVSQSLSQSTSQIITSATTLMGVLIMMISISWEMTVAAVLMLPVSMLLVLVV
                     VKRSQKYFVAQQKNLGYVNGYIEEVYGGHNVMKVFNAEEGAVEEFGKINGELYSSAWR
                     SQFLSGMMMPIMMFIGNLGYVVVTILGGWLAVRKIIDVGDILSFIQYIRSFTQPVNQL
                     AQISNVLQSTVAAAERVFEFLDEKEGEEDSAAAIRLEHVEGRVTFENVKFGYKEDETV
                     IEDFSADIRPGQKIAIVGPTGAGKTTIVKLLMRFYDIDDGKILIDGHDIVRFTRKELR
                     GMFGMVLQDTWLFSGTIMENIRYGRPDATDNEVIQAATAAHAHRFILTQPEGYNMIIN
                     EDASNISQGQKQLLTIARAILSDPKILILDEATSSVDTRTEVLIQRAMENLMKGKTSF
                     IIAHRLSTIRDADIILVMRDGDIIEAGNHEELLSRDGFYSSLYNSQFADTTGVS"
BASE COUNT         2008 a         1305 c         1605 g         1905 t
ORIGIN      
        1 ctcctaaaat aacccaccgc tataccgatt acgatgcttt caatcaacat tgcttttcca
       61 atttccttct ctcagtattt atcttttcag aaccttgaca aaggcttcta caacatcggg
      121 gtgaaactgt gtgccgctac cttcaatcag tatcccgact gctttcgtcg gggacatggc
      181 gtccctgtat ggacggttag aagtcatggc atcatacgcg tctgctgctg aaaggatgta
      241 tgcctctatg gggacttggt ctccgcctat gcctttggga tatccttttc catcatatct
      301 ttcatggtgg tacctaatta tatccttact ctttagggga agttcaatat cctctaatat
      361 ctttacccct attatggggt ggtgatgaat ttgggagaac tcggaactgt tgagtacctc
      421 tttcttattc aggattgaat catttatccc tatcttgcct atgtcatgga gaagcgctgc
      481 aacttcgata gaatgaatgg tggcgttgtc tagcctaagc tcatgggcaa tttggctggc
      541 atatccttct accctgcggg aatggccttc cgtgtaggga tccttcgcct ctatagcggc
      601 cgtgagggcc ttgaccgtct tatagtattg ttctttggaa ttttgatata gttggaagga
      661 atggcgggcc agaagcagcg ggcctaccaa taatgcaata taaattttgc caagggcctg
      721 gttgtagagc agcgcaacaa agtatccaaa aggcgcaatg gcagcaaaat tcggtagaat
      781 ccaagacata aggctgcccc aaagcttgaa aaacgaatcc ctgtttagca gtgacatcaa
      841 tgccatgaga attccagtat ttaaaagggc ataggaaagc gtgtatatca gcataagtag
      901 ggtaaaggct aactttgtaa tgtctaattc atatactttt gatagaaatt ctaatgctgt
      961 tcccgatgta aaagccgata tggagacatt agccacatta aaaagggttt tatatggggg
     1021 aacactgaaa atatgcctgt aggtctgggg tttttccttg tacacaaaaa aggtttggct
     1081 ccccgcggct ataatcacac tataatatgt tccataggtg agctggacag caaaaattat
     1141 cgcgaatacc acgcttacat atcgattctt attaaatacc accaaaaatg attggcaaag
     1201 accggctaaa agcatccaat atatcatatc gtacacattg ctttggcccg atattttgta
     1261 gagactaaac cccaagatga gaataccaac tgatataatt aacgataaaa aggctatggt
     1321 ctttttatcc atactccaac acctttcgaa acaaaaatag gccaaaccca tcagactaat
     1381 accaacctac gctagcgccg ccggcaagta tcagggaaag taatgcagac agtgcaacta
     1441 ataccttaac tgctatcttc ttcatctaga tatagcctcc tgttataaaa tctctgtcat
     1501 cggctagtca gacaggccat atccgctccg ctattaaact agaaaatatt aagaaatctg
     1561 taaatggttt ggcctattaa tggttttatt taaaataccg aacaatcctg cgattgatgg
     1621 catctagagt tgcctttacc actgtctcac tttcatcctt ggtgacgatg caacatccgc
     1681 ataataattc ctcacctaat tcgaacaggt gggatacggt taccatatag gcttttttat
     1741 ctgaaacata aacgggggtt atatccccga gaataaatac gtattcaatg tccaaagcat
     1801 tttgtatagc gtttaaggct gcttcggata ttaaacgtat gcggcttccc agcgaatttc
     1861 cgccctgagc catccccaaa aaggattccc catcttttaa taggattact tcgacttggg
     1921 atttgggtcc ttctgataac acttttacat aatgcagatt aaacctgtgc tctctgggac
     1981 caattagatc atcctctatt tgggcgatac ttatgatctt atggtcaatt tttaaattat
     2041 atgtagtaag catagcggat tgaatatccc taaccagctg ttttggagag cgataaatat
     2101 ccgccaagac gtggagttca atgatttcgc caccctggtt ggcaacgaac cgggaagaaa
     2161 taacaccggg aatcttattt attaaaacct cataatcagt caggccgctc aaaacgatca
     2221 ctcccgttat taggaaatta ttttacctta tttgcttaat tcgccaaata ctttgtgaat
     2281 ccttctttta aataattttt tttggctcat gtcccggtgc cgggatcaga ttattgaggt
     2341 tttcggatca tggctcggat atgcaaacat ttatattttt cagcttatgt tgactgggaa
     2401 aaacaggagt gttaatatga gagggaaaca tttttggata ttacctttta ggggaaaatt
     2461 atatttaagg ggctgttggg caaaatctaa gataatcttc aaatttatta gacgaagacc
     2521 gaagcccatg tcaagcggcg ggcaatcagg aaattggggt ggagaaatgt tacggatgat
     2581 aaaggaaatc aggccattta tcataacaat cttaataata attgcgttac tttttgtaca
     2641 ggctatggca gaattagccc tgccggacta tatgtcgagt atagtcaacg ttggaattca
     2701 gcagaagggt atagaagacg tcgtccctaa tgtgataagg gcggacagtc tggagaagat
     2761 aaaagccttt tcgagggaag atgagagaaa tcttatatcc cgcagctaca aactgctgga
     2821 caggggggat ttatcggccg aggaatatga gcattatgta acgaaatatc cagctctaga
     2881 aaaagaaaac ctttatattt tagatgcagc agataaggga gagattgaaa ggctgggctc
     2941 ttttcttggt aggacgatat taatagcgtg gggaatggat cagaatatgc caaatattga
     3001 aggggatact tcgcccttta atgacttgcc ccaagggacg gatccctttg agacgttgga
     3061 ggctgtgccc caagaaatgt tagacgaatt aagggcgaag gctgaagaat ggtttgatca
     3121 gatgccggat tccatgatta cccagtccgc gataagcttt ataagtaccg aatatgaggc
     3181 cataggggta aatatcccca aggctcaatc gaattatatc ttgaccatag gcggcataat
     3241 gttgctcatc gccctattgg ttatggcaac atctatactt gttggctttc tgtcggctag
     3301 ggtttctgct tcccttggta gaaatttgcg tgatggggta tttagaaagg ttgcatcctt
     3361 ttccaacgcc gagtttgatg atttttcaac tgcttccctt ataaccagga gtaccaacga
     3421 catacaacag gttcaaatgt ttgttgttat gctcttgagg atggtgtttt acgctccaat
     3481 catcggaacg ggtggggtga ttagggctct aaataccgat acttccatgg cttggatagt
     3541 cgcagtgggg gttatggcgg ttttaattct gattacggtt ctttttttca tcgccacacc
     3601 cagatttaag aggatgcaaa agctcgtgga tagggttaat cttgtcatga gggaatccct
     3661 caacgggatg atggtcataa gggcgtttaa tacccaaaga ttagaggaag ataagtttga
     3721 taaggccaat aaggatttga ccgagacaaa cctcttcgta tccaggctta tgacgatcat
     3781 gatgccggct atgatgctga ttatgaatgg gataatgctc ctaatcatct gggtcggtgc
     3841 gcatcaaatc gaagaaggaa ctatacaggt tggggacatg atggccttca tacagtatac
     3901 catgcagatt attatgtcct tccttatgat atccatggta tctataatgt tacccagggc
     3961 ttctgtatcc gtgcagagaa tctccgaggt tttagatagg gaaataatca tcaaagatcc
     4021 tttaagccat gtggaattag acggaggcgt aaaaggatgt atagaattta tggatgtata
     4081 ttttcaatat ccgggtgcgg aagaatgcgt tttagagggc ataacctttg tggcggagcc
     4141 gggccagacc acagctttta ttggtagtac aggcagcgga aagtctacat taatcaacct
     4201 tatcccaagg ctatatgaca tctcaagggg acgaattttg ttggatggga tagatattag
     4261 agatatgagg cttaaggatc taagggagga aataggatat gttccgcaag agggaatcct
     4321 tttcacaggt acaatagaga gcaatataaa atatggcaaa aatgctgtcg ttggcgaaga
     4381 agaggtttta aaatccatag aaattgccca ggcgaaagaa tttattgatg aaagagaggg
     4441 gggtatccaa tctgaaatat cccaaggggg gaccaatgta tccgggggac agaggcaaag
     4501 attgtccatc gcaagggcat tggcaaaggc gccaagggtg ttaatattcg acgacagttt
     4561 ttctgccttg gattttagaa cggatgctgc cctaaggaag gctatgagcc atgaaatagt
     4621 gggcagaacc gtactgatag tggctcaaag gatcaatacc ataatgaagg cagaaaaaat
     4681 agtggtattg gatgaaggga aaatagtagg gcagggaacc cacagagagc tcctaaagac
     4741 ctgtgaggta tatagacaaa ttgccctatc ccagttatcg gaggaggaac ttgccatatg
     4801 agcagagaga agacaaccag aaggtccgga aggataggcg gcgggtttgg cggtagaccg
     4861 ggatcttttg agaaacccaa aaactttaag gaaacctttt ccaagctcct caggtattta
     4921 aaaccctatg gactacagct ggttgtagtg gtaattttcg ctgtaggcag cgcagccttt
     4981 tcaatagtgg ggccgaagat actaggaagg gcaacggcta atatattcga gggactgata
     5041 gcaaagatat ccggtgttcc tgggggagga attgatttta gatacatagg aaatatcatc
     5101 cttattctta ttgggctgta tttactgtct gcgctatttt ctttggttca gggctttatg
     5161 gtcaccggta ttgcccagaa gatatcatat aacttgagga aggaaatatc gcaaaaggta
     5221 aatcgacttt ccctcaaata ctttgatacc atgacccacg gtgaggtatt gtccagggtt
     5281 accaacgacg tggataccgt cagccaatcc ctgagtcaaa gcaccagcca aataataaca
     5341 tcagctacaa cgcttatggg tgtactgatt atgatgatat ccataagttg ggagatgacc
     5401 gtcgcagctg tcttgatgct ccccgtgtcc atgcttttgg tcttggtggt ggttaaaaga
     5461 tctcaaaaat actttgtagc ccagcagaag aaccttgggt atgtcaacgg atatattgaa
     5521 gaggtatacg gcggtcataa tgttatgaag gtattcaatg ccgaagaagg tgccgttgag
     5581 gagttcggta agatcaatgg ggaactttat agctctgcat ggagatccca atttctatct
     5641 ggaatgatga tgccaatcat gatgtttatc ggaaacttag gatatgtggt cgtgaccatt
     5701 ttaggcggat ggctagccgt aagaaagatc atagatgtgg gagatatcct atcctttatt
     5761 caatatataa ggagctttac ccaacctgtt aaccagttgg ctcaaataag caatgtgctg
     5821 cagtccactg tggcagccgc agaacgggtt ttcgaattct tggatgagaa ggaaggcgaa
     5881 gaagatagcg ctgctgctat tagacttgag cacgttgagg gcagggtaac ctttgagaac
     5941 gttaaattcg gatataaaga ggacgaaacc gtcatagaag atttttctgc agatataagg
     6001 cccggacaaa aaatagctat agttggtcct actggagcag gcaagaccac tatcgttaag
     6061 ctccttatgc gcttttacga tattgacgat ggcaaaattt tgatcgacgg ccatgatata
     6121 gtccgcttta ccagaaagga attaaggggt atgtttggta tggtacttca ggatacatgg
     6181 cttttttccg gcaccattat ggagaatatc aggtatggaa gaccggatgc tacggacaat
     6241 gaggtcatac aagccgccac ggctgcacat gctcacaggt ttattcttac tcaacctgaa
     6301 gggtataata tgatcataaa tgaagatgcc agcaatatat cccagggaca aaagcagctt
     6361 ttaaccattg ccagggctat cctctctgat ccaaagatat tgatattaga cgaggcgaca
     6421 tcatcggtgg atacaagaac cgaggtgctt atacaaaggg ctatggaaaa cctcatgaaa
     6481 ggaaaaacat cctttataat cgcccatagg ctttctacta ttagggatgc ggacattatt
     6541 ctggtcatga gggacggaga tattatcgag gccggtaatc atgaggaact ccttagtagg
     6601 gacggatttt attcttcatt gtacaacagc cagtttgcag atacgacagg ggtatcctga
     6661 ctgtgattgg ggctaggaat acactatttt ttatatgtat cgtaaggggg atcaaacctt
     6721 atagggaatt tggtccccgg tgtaggagta acgaatttga aattaaaaat acccccttga
     6781 ctttagcgta gtaaagttat actatagcaa tgtagcaaaa acc
//
LOCUS       DASRNX010000042         5504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_274138,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000042 DASRNX010000000
VERSION     DASRNX010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274138"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1192
                     /gene="flhB"
                     /locus_tag="VFD89_01630"
     CDS             95..1192
                     /gene="flhB"
                     /locus_tag="VFD89_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HZJ56920.1"
                     /translation="MTLVDNMMDLQLFAGDKTEKATLKKRRDARERGQVFRSQEMNSA
                     IILLVSFTTLKFILPTLLEKIQKIYTEFLTDTAPIDQIFTHQGIVKLALDVIMTIGTA
                     IGPLLLISLAAGLAINYGQVGFLFTTKTIAPKFSKINPLEGFKRIFSRRSLVDLAKAI
                     IKLAIIGSIVYTEIKKNVGNVDLWYMGNFPWTVNLIGNNVFSVVIRIGIILFIFAVLD
                     YGYEWWQYETDLMMTKQEVKDEFKLTEGDPQIRSRIRGKQRELGMRRMMQEIPKADVV
                     ITNPTHYAIAISYDPDHDDAPIVIAKGQGHLAIRIKEVAQANGIVITENKPLARALYN
                     STDVGQMIPAELFHGVAEVLAFVYDLKGKTV"
     gene            1221..3245
                     /gene="flhA"
                     /locus_tag="VFD89_01635"
     CDS             1221..3245
                     /gene="flhA"
                     /locus_tag="VFD89_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HZJ56921.1"
                     /translation="MRFADNVIAFIVIIIVLLIILPLNQFVLDIFLTLNLSLSLLMLL
                     ISLYTKSALEFSIFPSLLLITTLYRLALNVSSTRLILIDGSAGKIIETFGRFVVRGNV
                     GVGLIIFLIIVVVQFVVITKGSERVSEVAARFTLDAMPGKQMAIDADLNSGLIDEREA
                     RTRRRDIQREADFYGAMDGASKFVKGDAIVGIIITVINIVGGIIVGFGKMEIQDVIST
                     YILLTVGDGLVSQIPALLISTATGIIVTKAASESSLGQDLLNQITSQPIVLIISGILL
                     CLMGFFPGFPAWLLFPLGGILIYLGFILLRSWGDDGIIEDDDIAQRELEDMRKPENVA
                     SLLSLDPIELEFGYGIIPLADVNQGGDLLDRVVMIRRQMALDLGLIVPVVRLRDNIQL
                     DPNEYVIKIKGTNVAQGQVMADRFLAMDPGLAEGEIEGVDTIEPAFGLPAKWIDEDHR
                     ERAELMGYTVVDPPSIIATHLTEIIKQYGHELLGRQEVQSLLDNLKESNSVLVEEVVP
                     KLLSLGELQKVLANLIRENVSIRDMVTILETLADYGSIVRDPDLLTEYVRQALSRTIT
                     QKFIPDNKGKVLTLDNQLEKEIMDSVQQTEHGSYLSMPPEKTQGVLKNLMDEINKVTS
                     LGMPPIILTSPVVRVYFKKLTEQVMPDLIVLSYNELDSRVEIQSIGVVNG"
     gene            3249..4370
                     /gene="flhF"
                     /locus_tag="VFD89_01640"
     CDS             3249..4370
                     /gene="flhF"
                     /locus_tag="VFD89_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HZJ56922.1"
                     /translation="MIIKRYIGNTTQEAMTQVKSDLGSDAIILNTRRIRKKGIIHWFA
                     KPLIEVTAAIDNDIVAHPKYGEKEIKDTKIAQTDHSYHGVEKKIDAMEEMIKQFVTGF
                     RHRPESGHHDTFGENQAYFDLLINNEVYRKYAVEIMERAMDLSARGGLEFSQCVEQII
                     LDCLGEEVKPIEIVPGQRKVVLFVGPTGVGKTTTLAKLAAGFAIEDGKRVGLITADTY
                     RIAAVDQLRVYAEILGIPMSVIYSPSDMPSALEEHGDRDIVLIDTAGRSIKDENHERE
                     ISEIIEYGNIQEVYLVISGNSSYQGCNDIIRSYEFLEDYNLIFTKIDEITTYGAIINC
                     SIMSRNPLSYVTTGQNVPADIEIANIHKIKDLLIGNKES"
     gene            4367..5260
                     /locus_tag="VFD89_01645"
     CDS             4367..5260
                     /locus_tag="VFD89_01645"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HZJ56923.1"
                     /translation="MKDQAEQLREIVARLQKRDRKENQGNARIITVTSGKGGVGKTSF
                     TANLGICLVQMGYRVIIIDGDFGLANIDLMLGMASRHGLREIILGQMDVEEVILEGPR
                     GIKFISGGSGIKELINLGPQQIDNFLGRIDKLNQMADIILIDTGAGAADNIVKMALAA
                     HEVVLVVTPEPTAITDAYALTKIITSIRRDIDLRLIINRVQNMAEAEDIFGKFTKTAH
                     RFLGVTIGTLGFIRDDLHVPKSIKEQVPYMVGYPNCDASRQVAHIAKKLTECDVIHGE
                     QNQGLVSFFRRFLGLFNFKDG"
     gene            5300..>5504
                     /locus_tag="VFD89_01650"
     CDS             5300..>5504
                     /locus_tag="VFD89_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake domain-containing protein"
                     /protein_id="HZJ56924.1"
                     /translation="MDLKSMIEIGGKIEIILKRDHVEDLRSFSMIQDISNEGDLIVTL
                     PMLEGRPILLNPADRIEIYFFKEE"
BASE COUNT         1711 a          951 c         1357 g         1485 t
ORIGIN      
        1 tggtctttat cccgatatat atcagctcac ttaatggaat attcgacgaa atgtttagcg
       61 atatgggcaa agccttgtgg aggatggtag gtagatgact ttggtggata atatgatgga
      121 tcttcagctt tttgcagggg ataagacgga aaaggcaacc ctgaaaaaga gacgtgacgc
      181 tcgggaaagg ggtcaggtgt ttcgcagcca ggagatgaat tctgctatta tattattggt
      241 gtcatttacc accctgaaat tcatcttacc caccctttta gaaaaaattc aaaaaatata
      301 tacagaattt ctcaccgaca ccgcccctat tgaccagata tttacccatc agggtattgt
      361 taaactggca ctcgatgtaa tcatgaccat aggtacggcc ataggacccc ttcttctgat
      421 atcccttgcg gccggtttgg caataaacta tgggcaagtt gggtttttat tcaccactaa
      481 aaccatcgcc cctaaattca gcaagataaa tcccttggaa ggctttaaga ggatattctc
      541 aagaagatct ttggtggatt tagctaaggc cataataaaa ctggctatta taggtagcat
      601 agtatatact gaaataaaaa agaatgtcgg aaatgtcgat ttatggtata tgggcaattt
      661 tccatggacc gttaatctga tcggaaacaa cgtatttagc gtagtaataa ggattggaat
      721 aatccttttt atctttgctg tgttggacta tggatatgaa tggtggcaat atgagaccga
      781 tttgatgatg accaagcaag aggtaaagga tgaattcaaa ttaaccgagg gggacccgca
      841 gattcgttcc cgaattaggg gaaaacaaag ggaattgggt atgcgcagga tgatgcaaga
      901 gataccaaag gctgatgtag ttatcacaaa tcccacccat tacgctatcg ccatcagcta
      961 tgatcccgat catgatgatg cgccgattgt aatcgccaaa ggtcagggac acttggccat
     1021 taggataaag gaagtcgccc aagcaaacgg gatagtgata accgaaaaca agcccctagc
     1081 aagggcactc tacaattcta ccgatgtggg ccagatgata cctgcggaat tattccatgg
     1141 ggtagcggag gtcctggcgt ttgtatacga ccttaagggc aaaactgttt aacgcttttc
     1201 cagttcaagg ggggtttatg ttgagattcg cagataatgt tatagctttc atagtaatca
     1261 taatcgtatt attgataatt ttacccctta atcaatttgt tttggatatt tttctaaccc
     1321 taaatctgtc attgtccctg cttatgcttc taatcagcct atatactaaa agtgcactgg
     1381 aattttctat ctttccttcc ttattgctaa ttaccacatt atatcggctc gctcttaacg
     1441 tatcctcaac caggttaatt ctaattgacg gatcagcagg gaaaattata gagacctttg
     1501 ggagatttgt cgttagggga aatgttggcg tgggcttaat aatttttctc attatagtgg
     1561 tagtacaatt cgttgtaatt actaaggggt cggaaagggt ttcagaggta gccgccagat
     1621 ttactttgga tgccatgccc ggtaaacaga tggctattga tgcagacttg aattcaggcc
     1681 tcattgatga aagagaggca aggaccagga gacgggacat tcaaagggaa gcagattttt
     1741 atggggccat ggacggtgcc agtaagttcg taaaagggga tgccattgta ggtattataa
     1801 taactgtcat caatatagta ggcggaataa tagtcggttt cggtaagatg gagattcagg
     1861 atgtaatatc tacatatatt ctgctaaccg tcggggacgg attggtaagt cagatacccg
     1921 cattacttat ttcaacagct acaggtatta ttgttacaaa ggctgcttcc gaatccagtc
     1981 tgggacagga tttacttaac caaattacca gccaaccaat tgtactaatc atatccggga
     2041 tactcctatg tttaatgggt ttctttcccg gctttcccgc atggctccta ttccccttgg
     2101 ggggcatact gatatactta ggttttattt tgctgagatc ttggggggac gacgggatta
     2161 ttgaagacga cgatatagca cagcgggaat tggaggatat gcgcaaaccg gaaaatgtgg
     2221 cgagtttgtt atcccttgac cctattgagc tagaattcgg atatgggatt attcccttgg
     2281 cggatgtaaa tcaaggcggg gacctattgg atagggttgt aatgattaga agacagatgg
     2341 ctttggactt ggggcttata gtccctgtag tgcgcttgag ggataacata cagctggatc
     2401 ccaacgagta tgtgataaaa ataaagggta caaatgtagc ccaagggcag gttatggcag
     2461 atcgcttttt ggctatggat ccgggtttag ctgaaggaga aattgaaggt gtagacacta
     2521 ttgaaccggc ctttggatta cccgcaaaat ggatcgatga agatcatcgt gagagggcag
     2581 agcttatggg gtatacggta gtagaccctc cttcgataat agctactcat ctgacggaga
     2641 ttataaaaca atacggccac gaattattgg gacgacaaga ggtacaatcc ctattggaca
     2701 atctaaagga gagcaattca gttttggtgg aagaggttgt tcctaaactg ctatcccttg
     2761 gggagctcca aaaggttttg gcaaatttga taagggagaa cgtgtctata agggatatgg
     2821 ttaccatttt agagacctta gctgattatg ggagcatagt aagggatccg gatttattga
     2881 cggagtatgt cagacaagcg ctttctagga ctataaccca gaagttcata cccgacaata
     2941 aaggtaaagt tctaaccttg gacaatcagc tggaaaaaga gatcatggat agtgtgcagc
     3001 agacagagca tggatcctat ctttctatgc cacctgaaaa aacccaggga gtattaaaaa
     3061 atctgatgga tgagataaat aaagtaacat cgttgggtat gccccctatt attttgacct
     3121 ctccggtggt tagggtttat tttaaaaaat tgaccgaaca ggttatgccc gatctaatag
     3181 tgctatccta taacgaattg gacagcaggg tagaaataca atctataggg gtggtgaacg
     3241 ggtaatctat gataattaaa cgttatatag gcaatacaac ccaggaagca atgacccagg
     3301 taaagtcaga tctgggatcc gatgcgatta tactgaatac caggcgcata aggaaaaaag
     3361 gcattattca ttggtttgca aagccgctga tcgaggttac cgcggcgatt gataacgata
     3421 tagttgccca tccaaaatat ggggaaaagg aaatcaaaga taccaagata gcccaaactg
     3481 atcattccta tcatggggta gaaaaaaaga ttgatgcaat ggaagaaatg attaaacaat
     3541 ttgtgacggg gttcaggcat aggccggaaa gtggacatca tgataccttt ggtgaaaatc
     3601 aagcctactt tgatctacta attaataatg aggtatatag aaaatatgca gttgagataa
     3661 tggaaagggc tatggattta agcgccagag gggggttgga attttcccaa tgcgttgaac
     3721 agataatcct ggattgtctg ggtgaggaag tcaaacccat agaaattgtc cccggacagc
     3781 ggaaagtagt cctttttgtg ggacctacgg gcgttggcaa aactacaaca ttggcaaagt
     3841 tggcagccgg ctttgccata gaggatggga aaagggtagg gttaataacc gcagatacat
     3901 atcgaatcgc agcagtggat caattaaggg tgtatgcgga aattttaggg attccaatga
     3961 gtgtcattta ttctccaagc gacatgccct ccgcccttga agagcatggg gacagggata
     4021 tagtcttaat agatacagcc gggagaagca taaaggatga aaatcatgag agggaaataa
     4081 gtgagatcat agaatatgga aacatccaag aagtctatct ggtgataagc ggtaattcga
     4141 gctatcaggg ttgcaacgat attataagga gttatgaatt ccttgaggat tataacctta
     4201 tatttaccaa aattgatgag ataaccacct atggggctat aataaattgc agcattatgt
     4261 ctcgcaaccc cctttcatac gtaacaactg gtcaaaatgt gccagcggat atagagatag
     4321 ccaatataca taagataaaa gatttgctga taggaaataa ggaatcatga aagaccaggc
     4381 tgaacagctc agggaaatcg ttgccaggct gcagaaaaga gataggaaag agaatcaggg
     4441 taatgccaga attattacgg taaccagcgg taaaggcggg gtaggcaaga ccagttttac
     4501 cgcaaatctg ggtatttgct tggtccaaat ggggtatagg gtaataataa tagatgggga
     4561 ttttggttta gccaatattg atttaatgct gggtatggcc tctaggcatg ggcttaggga
     4621 aattatttta ggtcaaatgg acgttgaaga ggtaatacta gagggacccc gggggataaa
     4681 gtttatttca gggggatctg gcataaagga acttattaat ctaggaccac agcagataga
     4741 caattttctt gggaggattg ataaattaaa tcaaatggca gacataattt taattgatac
     4801 tggggctggt gcggcggata atattgtaaa gatggcttta gcagcccatg aggttgtgct
     4861 ggtagtcacc ccggagccaa ccgccattac tgatgcctac gcactcacaa agataataac
     4921 atcaattagg cgagatattg atctacgtct gataataaac agggttcaga atatggcaga
     4981 ggccgaggac atattcggaa agtttactaa gacagcccac aggtttttag gtgtaaccat
     5041 cgggaccctt ggatttatac gtgatgacct tcatgttcca aagtcgatca aggagcaggt
     5101 tccatatatg gtgggctatc caaactgcga cgcatcaagg caggtggcac atattgccaa
     5161 gaaattaaca gaatgcgacg taatccatgg ggaacaaaat caaggccttg tatccttttt
     5221 taggagattt ctgggtttgt ttaatttcaa agacggatag ggggataagc tatcaaattc
     5281 ttgtgaagtt gggtgaaaaa tggatcttaa aagtatgatc gaaatcgggg gaaaaatcga
     5341 aattatattg aaaagggatc acgtagagga tttgaggagt tttagtatga ttcaagatat
     5401 ttcaaacgaa ggtgatctca tcgttactct tccaatgctt gagggaagac ctatactatt
     5461 aaatccggca gataggatag aaatatattt tttcaaagaa gaag
//
LOCUS       DASRNX010000043         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_751517,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000043 DASRNX010000000
VERSION     DASRNX010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751517"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="VFD89_01655"
     CDS             <1..1343
                     /locus_tag="VFD89_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HZJ56925.1"
                     /translation="DKIDLEIPFGKTVEAGEFVSDIKDATQVTVSFLEEPDFEFIGEQ
                     DVDVVLLDTGKNRTIYTAGLYISKVYKTVAVELGTVRLDISDFLMDDAFKGDFITDIS
                     NINLKEPGTHDIALEVDGDRYLSQLEIIDTTPPRGNTVNRVIWLGEEISPEDFVEKVF
                     DYTDVKVSFDTEPDFNKIGVQKVSVVLEDTSENKTNLIAYLTIRRDTEPPIIEGAEDM
                     TVFVDERVSYRKNIRVTDNRDNDVELIIDSSKVNLKKEGEYEVTYIAVDTAGNTATET
                     ITITVLKKPWDYVDPEELDKLADSVLTSILREGMSDKEKLKAIFTWTKERISYTGSTY
                     KENWMRGAVQGIKKGSGDCFTYYGTARALLTRAGFENLCITRIGDTHYWNLVKYEGNW
                     YHFDACPHNDRYPFDGFLRTDAEVERYSKKRKDKRGYYNFDKSLYPATPDEPLG"
     gene            1551..2000
                     /locus_tag="VFD89_01660"
     CDS             1551..2000
                     /locus_tag="VFD89_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HZJ56926.1"
                     /translation="MDKWDKRFMEMVKVIAGWSSCYQYNRKIGAIIVKNKRILTTGYN
                     GAPSGYPSCVERQECMRRIRGIPSGTRHEYCYAIHAEQNAIVQAAKLGVSIDGATLYC
                     THQPCVICAKLIVNSGITRVVYGEAYPDSLATEILMGVGVSLEKFIE"
     gene            complement(2014..>2475)
                     /locus_tag="VFD89_01665"
     CDS             complement(2014..>2475)
                     /locus_tag="VFD89_01665"
                     /inference="COORDINATES: protein motif:HMM:NF039337.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HZJ56927.1"
                     /translation="LLGKKKDSNNPVSTFLKWLDEHHHSCMICDRIANLLDRYAFTII
                     HLWQKDKDFKGTLADSKGFCLHHFWLMTKIAHETLSMRIFDRWIDFILPIQTETLNQL
                     DADLLWFTQKFDYRNQDKPWGNSRDALPRTLQTLSGKYMEPPAPPNEDNED"
BASE COUNT          731 a          465 c          627 g          652 t
ORIGIN      
        1 ccgacaagat cgatctggaa attccttttg gaaaaacagt cgaggcaggg gaattcgttt
       61 ccgatataaa ggacgctacc caagtgactg tttcttttct ggaggaaccg gatttcgaat
      121 ttataggcga acaggatgta gatgtagtat tgttggatac cggtaaaaac agaaccatat
      181 atacggcggg gctttatata agcaaggtgt ataaaacggt agccgttgaa ttgggtacgg
      241 taaggttgga tatctccgac tttttgatgg atgacgcctt caagggtgac tttataaccg
      301 acatttccaa tatcaatctt aaagagccgg ggacccacga tatcgccttg gaagtcgatg
      361 gagacaggta cctgtcccag ctggagatta ttgataccac cccgcccagg ggcaacacag
      421 taaaccgggt gatatggctg ggtgaggaga tatcccccga ggattttgta gagaaagtat
      481 tcgattacac cgatgtgaaa gtttcctttg atacggagcc ggatttcaat aagatcggag
      541 tgcaaaaggt atctgttgtt ttggaggata ccagcgagaa caagacgaat ttgatcgcat
      601 accttacaat aagaagggat acggagccgc ctattataga aggcgcggag gatatgaccg
      661 tctttgtgga cgaaagggtc tcctatagaa agaatatacg ggttacggat aacagggata
      721 acgatgttga attaatcatt gacagcagta aggttaattt aaaaaaagaa ggggaatacg
      781 aggtaaccta catagctgtc gatacagccg gaaacacagc taccgaaact ataaccatta
      841 ccgtcttgaa gaaaccatgg gattacgttg atccggaaga attagataaa ctggccgaca
      901 gcgttttgac atcgattctg agggagggaa tgtccgataa agaaaagctc aaagccatct
      961 ttacatggac taaagaacga atctcatata ccggcagtac ttacaaggaa aattggatga
     1021 ggggtgctgt ccaaggcatt aagaaagggt ccggagattg ttttacctat tatgggacgg
     1081 ccagggcttt gttgactagg gccgggtttg aaaatttatg tattaccagg ataggcgata
     1141 cgcattactg gaacctagta aaatatgagg gtaattggta tcattttgat gcatgtcccc
     1201 ataacgacag gtatcctttt gacggattcc tacggacgga tgcggaagta gaacgatact
     1261 ctaaaaagag gaaagacaaa aggggctatt ataactttga caaaagcctg taccccgcga
     1321 caccggatga accgttggga taggttagac tgtacgcttt ttggtaatcg acttgcacat
     1381 accttggata ccgcatcatt tcttctttgg cttatatgtt acgaaaaaca cattgattaa
     1441 gacaatatca gaaggtctga ttggtcatgc aatgacagga ctttttcgta acatatagat
     1501 atttttgggg tcttatgtaa tcgattgatt tttataatgg gggatttggc atggataagt
     1561 gggataagag atttatggaa atggtaaagg taatcgctgg gtggtccagc tgctatcaat
     1621 ataacagaaa aatcggcgcc atcatagtga agaataagag gatattaacc actggttata
     1681 acggtgcccc ttcgggatat cccagctgtg tagaacggca ggagtgtatg aggaggataa
     1741 ggggaatccc atcgggaacc aggcatgaat actgttatgc cattcatgct gagcaaaacg
     1801 ccatagttca ggcagcaaaa ttgggggtat ccatagacgg tgctacccta tattgcaccc
     1861 atcaaccctg tgttatctgc gcgaagctga ttgtgaacag cggcatcact cgggttgtat
     1921 acggggaggc ttaccccgac agtctggcta cggaaatact gatgggggtg ggagtcagcc
     1981 tggaaaaatt tattgaatag atggagccgg gtatcaatcc tcattatctt cgttgggtgg
     2041 ggcaggcggt tccatatact tgcccgataa tgtttgaagg gtcctgggga gagcatccct
     2101 ggaattaccc caaggcttgt cctgatttcg atagtcgaat ttctgtgtaa accataatag
     2161 gtccgcatcc aattgattca aggtctctgt ttggatcggt aaaatgaaat cgatccacct
     2221 gtcaaagatc ctcatggaaa gggtttcatg cgctattttc gtcatcagcc aaaaatgatg
     2281 gagacaaaac cccttcgaat ctgccagagt ccctttaaaa tccttatcct tttgccagag
     2341 atgaataatg gtaaatgcat atctgtccaa aaggtttgct atcctatcgc atatcatgca
     2401 gctatggtgg tgttcgtcca accattttaa aaaggtgctt acggggttat ttgaatcctt
     2461 tttcttcccg agcag
//
LOCUS       DASRNX010000044         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_115082,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000044 DASRNX010000000
VERSION     DASRNX010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115082"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1099)
                     /locus_tag="VFD89_01670"
     CDS             complement(59..1099)
                     /locus_tag="VFD89_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56928.1"
                     /translation="MEQFALIGMPDGGHRYIQSHPLLKFLPVKWAMAMIPRVKPRFTE
                     IIYDKENRNELGYILDVPGFFIDKDKHGESNRTQNINNLVGLLLEKGIGVLVFPLWRN
                     YISAEERSFIEESITILDGGLIKLVSLMDTVEKLFVILNAKPNEIEIGVWGADNAVGR
                     IWTRLLAPRINYLVIGGEDKEALVELGSEVFHDTGLSCHISTDPSTCLSNKSMAILSA
                     LPKEWVDPGNTRLIILASRLADSSPYIGENNKDVISIESGWFDLPKAMELKKSLGVWE
                     RMGITEAMLFIRDSSYRNLFTKKALTLENLRIMKGIIEEHGIGFSNMISNESILSYNG
                     FRRLYFGNFLDK"
     gene            complement(1126..2031)
                     /locus_tag="VFD89_01675"
     CDS             complement(1126..2031)
                     /locus_tag="VFD89_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HZJ56929.1"
                     /translation="MKRIVSISIGSSTRDHRVETEILGNKFLIERIGTDGDIKKAIEI
                     VKGLDGKVDVFGMGGIDLYLYGRRNKRYMLRAALPILKAAGNTPIVDGSGLKNTLERR
                     VVRFVNDECNIPFKDKRVLLVCGMDRFGMAETFETLGSKVTYGDLIFALGVKIPINSL
                     RGLHTIAGILMPVVSRMPFKFLYPTGENQNQNIPKYHEYYQNADIIAGDFIYIRKHIP
                     LEMEGKIIITNTVTLDDISFLKERGIKMLITSTPELNGRSFGTNVIEAVFVSLANRGA
                     QEVTPGAYEDLIKRSGFKHRVEMLN"
     gene            complement(2079..3062)
                     /gene="dusB"
                     /locus_tag="VFD89_01680"
     CDS             complement(2079..3062)
                     /gene="dusB"
                     /locus_tag="VFD89_01680"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZJ56930.1"
                     /translation="MIKKDLVIGSLQLPNNILLAPMAGITDLPFRLICKGYGCGMVFT
                     EMVSAKGLYYGSERTEDLLLIHPAEKPIGVQIFGSEPRIMGEMADRISDLDIGLIDIN
                     MGCPAPKIIKGGQGSALMRDPERVRKIVKAVVKSSSKPVTIKIRKGWDDAEVNAVEIA
                     KIACDEGASAITVHGRTREQFYSGKADWEVIKRVREAVHIPVIGNGDVFTPEDAFKMF
                     DYTGCHGIMVARGAQGRPWIFRDIIHYLKTGSRLLEPPATERISIAVRHLKLAVELKG
                     ERLGVIEMRKHLAWYLKGMKNVNTIKNTINSLENSEQIQNTLFEYLEKEYN"
     gene            complement(3136..3675)
                     /locus_tag="VFD89_01685"
     CDS             complement(3136..3675)
                     /locus_tag="VFD89_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56931.1"
                     /translation="MNTLLRLLSYILTRLTVFAIIAILIILAVFIGYDWANVYVITNE
                     GLAQRAETVIKGQDASELIKFYTQDFLNRDPLLARAPYEKFDVNNFDHRVKIKMLWVW
                     PWENQAKVNVEEVITGIDGSKKDEESEDIPIPSWENGEKLIIMEKDGRWKIGNVVMTK
                     PIKVESKEEKDKIKDGVQE"
BASE COUNT          968 a          970 c          642 g         1202 t
ORIGIN      
        1 aaagcggcaa cagtgctttt tcccctaatt tttgaataca ttatagaata gcatccttct
       61 atttgtcaag gaaatttcca aaataaagcc ttcgaaaacc attatatgat aggatgcttt
      121 catttgatat catgtttgaa aacccaattc catgttcctc tattatacct ttcattattc
      181 tgaggttttc cagcgtgagc gctttttttg taaacaggtt ccgatatgag ctgtcccgga
      241 taaaaagcat cgcctccgtt atgcccattc gttcccacac cccaaggctt tttttcagtt
      301 ccattgcttt tggcaagtcg aaccagcccg attctattga tataacatcc ttattgtttt
      361 ctccaatata aggagatgag tcagctagcc gggatgccaa tataattaat ctggtattgc
      421 ccgggtctac ccattcttta ggtagcgcgc ttagaattgc catggatttg ttgctaagac
      481 aagtgcttgg atccgtagat atatgacatg ataagcctgt atcatgaaaa acttcactac
      541 ccagttccac taaggcttcc ttatcttctc cgccgatgac taaatagttg atccgaggtg
      601 ccaggagccg ggtccatata cggcctacag cgttgtccgc tccccaaacg cctatctcaa
      661 tttcattggg cttggcgttt agtataacaa ataatttctc taccgtatcc ataagtgaga
      721 ccagcttgat caagccgccg tccaaaatgg ttatggactc ctctataaaa ctcctttcct
      781 ctgccgaaat gtaattcctc cacaggggaa aaacaagaac acctatcccc ttttccagaa
      841 gcaatccaac tagattgttt atattttggg tccggttcga ttcaccatgt ttgtccttat
      901 caataaaaaa accgggtaca tccaagatgt atcccagctc gtttcgattt tccttatcat
      961 atataatttc agtaaacctt ggctttaccc tggggatcat cgccatagcc cattttacgg
     1021 gtaaaaattt taaaagggga tggctttgta tatatctatg ccccccatcc ggcataccta
     1081 tcagcgcgaa ctgctccatg tattacggcc cctttcacat taatactaat tcagcatctc
     1141 aacccgatgt ttaaacccgc tccttttaat tagatcctca taagcccccg gtgtaacctc
     1201 ctgagccccc ctattggcta agcttacaaa caccgcctca attacattgg taccaaagga
     1261 cctcccgttt agttcggggg tggaagtgat cagcatcttg attccccgtt cctttaaaaa
     1321 gcttatatcg tcaagggtga cagtgtttgt gataattatc ttgccttcca tttcaagtgg
     1381 aatatgcttt cttatatata tgaaatcccc ggctataata tcagcattct ggtaatattc
     1441 atgatacttt ggtatgtttt gattctggtt ttcgccggtg ggataaagga atttaaatgg
     1501 cattctgctt actacaggca ttaggatacc ggctatggta tgcaggcccc gcaaactatt
     1561 tatgggaatt ttgacaccca aagcgaatat taaatctccg taggtgacct tgctgcccaa
     1621 ggtttcaaag gtctccgcca ttccaaatct atccattccg catacaagaa gcaccctctt
     1681 atctttaaag gggatattgc attcatcgtt gacaaacctc acaacccttc tttccaaggt
     1741 gtttttgagg cctgatccgt ccacaatcgg cgtattgccg gcggctttaa gaatgggtaa
     1801 ggcggctctt agcatatatc gcttgttcct tcgcccatag agatatagat cgatgccccc
     1861 cataccaaat acatccacct taccatccag gccctttact atctcaatag ccttttttat
     1921 atcgccatca gtacctattc gctctattaa aaacttgttt ccgagaattt cagtttcgac
     1981 cctatgatcc ctggttgacg acccaatact tatgcttaca attcttttca taagtacccc
     2041 ctttacatat caggtacaac agtgacacac ttcctgcctt aattatactc cttttccaag
     2101 tattcgaaaa gggtgttttg tatctgttcg ctgttttcca aggaatttat tgtattttta
     2161 atggtattta catttttcat gccctttaaa taccacgcca ggtgctttct catctctatt
     2221 acccccagcc tttccccctt aagctccact gccagcttta ggtgcctgac tgcaatactt
     2281 atcctttccg ttgcgggtgg ttctaacaat ctgctgcctg tctttagata atgaataatg
     2341 tcccggaata tccaaggccg cccctgggcc cccctagcaa ccattatccc atggcatccc
     2401 gtatagtcga acattttaaa ggcatcctca ggggtaaaaa cgtccccatt tcctatgacg
     2461 ggtatatgga ctgcttctct aactcgtttt attacctccc aatctgcctt ccctgaatag
     2521 aattgttccc gtgtcctgcc gtgtactgta atggccgatg ccccctcatc gcaggctatc
     2581 ttagctatct cgaccgcatt tacctctgca tcatcccaac cttttcgaat ctttattgta
     2641 accggcttgg atgagctttt tactacggct ttgactattt tacgaaccct ttccggatcc
     2701 ctcataaggg ctgatccttg ccctcctttt attatcttcg gcgccgggca ccccatattt
     2761 atatcaataa gccctatgtc cagatctgat attctatctg ccatttcccc cattatccgg
     2821 ggttctgagc caaagatttg gacacctatg ggcttttcag ccggatgtat caacaataga
     2881 tcctcggtcc tttcgctgcc atagtataga cctttggcgc tcaccatctc agtaaagacc
     2941 ataccgcatc cgtatccctt gcatatcaat cggaaaggta aatccgttat tcccgccatg
     3001 ggagccaata atatattgtt aggcaactgc aaactaccta taactaaatc cttctttatc
     3061 atgtaatgtc cctttcccga gtgaaatagt tttaaaaggg gggatttaat agaaatagcc
     3121 gcattgctct gcggcttatt cttgtacccc gtccttaatt ttatccttct cttctttgct
     3181 ttccaccttg atgggcttag tcataactac attgccaatc ttccatctcc catccttttc
     3241 catgatgatc agcttttccc cgttctccca agaaggaata ggaatatctt cggattcttc
     3301 atcttttttg ctgccatcaa taccggtaat tacctcttca acattgacct tagcctgatt
     3361 ctcccatggc catacccata gcatcttgat ctttacccgg tggtcaaaat tattgacatc
     3421 aaatttttca tatggtgccc tagctaataa cggatctcta ttgagaaaat cctgtgtgta
     3481 aaatttgata agttcagagg catcttgacc cttaattaca gtttctgcac gctgggccaa
     3541 accttcattg gtaatcacgt agacattagc ccaatcatag ccgataaaga cagccaaaat
     3601 gattaatata gctataattg cgaacacagt caacctagtt aaaatatatg ataaaagacg
     3661 caacaatgta ttcaacaccg gacccccgtt ctttgctaca ctccactttg tcgccattat
     3721 ccatatgttt tacgacaaac actccatatt gatataggtg tcgtaaaaca ttacccttaa
     3781 tg
//
LOCUS       DASRNX010000045         4575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_593495,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000045 DASRNX010000000
VERSION     DASRNX010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593495"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..402)
                     /locus_tag="VFD89_01690"
     CDS             complement(13..402)
                     /locus_tag="VFD89_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56932.1"
                     /translation="MKIVVISMINRLLSGAIIKYLRERGELMPIRVMDCSKKNEPYIT
                     CKSHEANILLMEVMKISPFTLEERLKTAAKMKKSLPHCKIVVLCDENADPDMAEKVKE
                     AKKMGFIDSFFYSSVSGEYLAAMLDAL"
     gene            complement(527..1333)
                     /locus_tag="VFD89_01695"
     CDS             complement(527..1333)
                     /locus_tag="VFD89_01695"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZJ56933.1"
                     /translation="MGKMMKNAINFFIAIIIILMVVIPVFADEELEFITDNAYLLTED
                     EYWELNSRARDISEKYECDISIITVEDMGSGDVYGYADSIRKTYGMGYGDGESILLLF
                     VSMEYRDYALIAHGYGNTAFTDYGKDMILDKHVLPLMGKEKYYKAFSKYLSIAEEYLK
                     MSRNGAPFDIKTDPNYGKLSLPIKAGVTFLIPLIIAAIICSLWKSKMKTAVLATTASN
                     YITADGLVLTNSQDHFLYRTESRRRIENKSTSSGGTTRDRSGVSGRSGKF"
     gene            complement(1333..2433)
                     /locus_tag="VFD89_01700"
     CDS             complement(1333..2433)
                     /locus_tag="VFD89_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56934.1"
                     /translation="MATQITNYQCPNCTGPIHFVGDTGLLKCDYCGTEYQVEIIEQIY
                     ADKAEAAASLGTETRWDMTMAGGNWNEEEAKRLRAYSCPSCGAELIMDDTTAATSCPY
                     CNNPTIVPGQFTGQLKPDYVIPFKLDKEAAVTSLKKYYKGKRFLPKSFSGGNHIEEIK
                     GVYVPFWLFDGKADASMRFRGTKVRTHRSGDVEVTITEHFRVLREGNIAFEKIPVDGS
                     SKMPDEHMDAVEPFNYGELKTFSIAYLPGFMADKYDMDANTCSERANSRIKKSAEDVM
                     ASTTMGYSTLVPEYSQIDLEQGEVKYALMPVWMLSTKWNDKTFLFAMNGQTGKLIGDL
                     PVDRGLYWSWFAGISLPLMAILGYFMFLGGIF"
     gene            complement(2459..2983)
                     /locus_tag="VFD89_01705"
     CDS             complement(2459..2983)
                     /locus_tag="VFD89_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56935.1"
                     /translation="MMSSFSTGINKNRKVRFNKIPTSLRELKYLPEAALKDPYHTAAL
                     LIPALCLWPTNRNESIDMINFLRGPGPLSTYEVQFISERLRENEYIPMSYLEGANAEN
                     GYRANEPFTVVVSAVPSSFTEKGYAMLYLKSGGADSMRPVKLREKPSTGQWFLTDQML
                     LAQIRKPISEDPWA"
     gene            complement(3199..3609)
                     /locus_tag="VFD89_01710"
     CDS             complement(3199..3609)
                     /locus_tag="VFD89_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56936.1"
                     /translation="MARESNKFLKVTGILMIIGGSFGVIFGIIAVIGVGAAAALLGSE
                     AKMGLLVLSSVLVLIGGIISLVAGIIGVKNAAIPEKAQTCIVFGILAAAVSVGGNLLK
                     VAGGGKFNFLGILTGLVLPVLYLIGAYQNKSKIA"
     gene            complement(3731..4498)
                     /locus_tag="VFD89_01715"
     CDS             complement(3731..4498)
                     /locus_tag="VFD89_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016149104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HZJ56937.1"
                     /translation="MAFFGRKNCDICGSKIGMLGNRKLEDGNLCKDCAKHLSPFFSDR
                     RRSSVADIKAQMDYREANKTQVGAFNVTRTLGVGTKILLDEDEAKFIVSSSRRWQEEN
                     PDVLDYSQVTGCDLEINESQTELKRKDRQGNEVSYNPPRRVYHYDFWMTIHVNHPYFS
                     EIRFKLNSSAIDIEPPQGRIFIQGGADVGRRSAEYQQCEATAEDIKETFTKVREGVRK
                     DIATANAPKTAQTCSFCGATTIPDMQGRCEFCGGAIS"
BASE COUNT         1233 a         1137 c          810 g         1395 t
ORIGIN      
        1 ttgttcaaaa ccttaaagag catccaacat cgcagccaga tactccccgc tgacggaact
       61 ataaaaaaaa ctatcgataa accccatctt tttggcctcc tttaccttct ccgccatatc
      121 ggggtctgcg ttttcgtcgc agagcacgac tatcttacaa tggggcagtg atttcttcat
      181 tttcgcagct gtttttagcc tctcttccaa ggtaaaaggt gaaattttca tgacttccat
      241 caaaagaatg ttggcctcat gggatttgca ggtaatatag ggttcatttt ttttgctgca
      301 atccattact cggatgggca tcagctcacc acgctccctc aaatatttga tgatagcacc
      361 agagagcagg cgatttatca tactaatcac aacaattttc aatccatcct acctcctcaa
      421 attccccata ccatctctta attatcctat caagggtcct attcatagat tattccaaaa
      481 tcggagatgc ccgttagcat acctcccatt ttaaagatta tatctgtcaa aatttgcccg
      541 agcgccctga aactcctgat ctatctcttg tagtgccgcc ggatgaagtt gatttattct
      601 ctatcctcct tcggctttca gtacgataga gaaaatggtc ttggctattc gttagcacta
      661 atccgtccgc agtaatataa ttgcttgcgg ttgttgccaa aactgcagtt ttcattttgc
      721 ttttccacaa actacagatt atagccgcta taattaaagg tatcagaaat gtaacccctg
      781 ctttgatcgg caatgaaagt tttccgtaat tggggtcggt cttaatatca aaaggagccc
      841 cgttccttga cattttcaga tattcctctg caatgcttag atatttggaa aaagctttat
      901 agtacttctc tttgcccatc agggggagca catgcttatc cagtatcatg tccttaccat
      961 aatcggtaaa ggctgtattg ccatatccat gtgctattaa ggcataatcc ctatattcca
     1021 tgctcacaaa taaaagtaag atactttccc catcgccata tcccatccca tatgtctttc
     1081 tgatggagtc tgcataacca tagacgtccc cggatcccat atcctctaca gtgataatgg
     1141 atatatcgca ttcgtacttt tctgatatat ctcgggccct actattgagt tcccaatact
     1201 catcttcagt tagcagatag gcgttatctg taataaattc aagctcctca tctgcaaaga
     1261 caggtattac aaccatcaaa ataatgataa ttgcaataaa gaaattaata gcattcttca
     1321 tcattttgcc cattagaata taccccctaa gaacataaaa tagcctagta tagccataag
     1381 aggtagtgat atcccagcga accatgacca gtacagacct ctatcgacgg gtaaatctcc
     1441 gatgagcttc cctgtctgtc cgttcatagc aaacaagaag gtcttatcgt tccatttggt
     1501 tgatagcatc cacaccggca tcagggcata tttaacttcc ccttgttcca agtctatttg
     1561 cgaatattcc ggcaccagcg tgctgtagcc catagttgta gatgccatga catcttccgc
     1621 gctctttttt attctgctat ttgccctttc cgagcaagta ttggcatcca tatcgtattt
     1681 atctgccata aatcccggca agtatgcaat ggaaaatgtc ttcaattctc catagttaaa
     1741 aggttccact gcatccatat gttcatccgg cattttactg gatccgtcca cgggaatctt
     1801 ttcaaaggca atattaccct cccttagtac gcgaaaatgc tctgtaatag taacttccac
     1861 atccccagat ctatgggtgc gtacttttgt tcctcgaaag cgcatactgg catcggcttt
     1921 accatcaaac aaccaaaacg ggacatagac acccttgatt tcttctatat ggttccctcc
     1981 ggaaaaagat ttcggcaaga atctcttgcc cttataatac tttttaaggg atgtcacagc
     2041 ggcttcttta tccagcttaa atggaataac ataatccggt ttgagctgcc cggtaaattg
     2101 accgggtact atggttggat tgttgcaata cggacaggaa gttgcggcgg ttgtatcatc
     2161 cataataagc tctgcaccac aagacggaca ggaataagcc ctcaaccgtt ttgcttcctc
     2221 ttcattccag ttgccgccag ccatagtcat atcccatcgc gtttctgtgc ctaaggatgc
     2281 cgcagcttct gctttatctg catatatctg ttctattatc tccacctgat actctgttcc
     2341 acaataatca cattttagta gaccggtatc ccctacaaaa tgtatgggtc ccgtacaatt
     2401 gggacattga tagtttgtga tttgggttgc caatttaata ccccctcaaa atcttcctct
     2461 aggcccatgg gtcctccgag atcggctttc ttatctgtgc aaggagcatc tgatccgtca
     2521 gaaaccattg gcctgttgac ggtttttccc gtagcttaac cgggcgcata ctgtctgccc
     2581 ctccggattt aaggtaaagc atggcatatc ctttttcagt aaagctggaa ggaacagcag
     2641 aaaccaccac agtaaagggt tcattagccc tatatccatt ttcagcgttt gccccctcaa
     2701 gataagacat tggtatgtat tcattttccc gcagtctttc tgatatgaat tggacttcat
     2761 atgttgataa gggtccgggg cccctcaaaa aattaatcat atcaatagat tcatttctgt
     2821 ttgttggcca taagcataat gccgggataa gaagagcagc agtatgatac ggatctttta
     2881 gggccgcctc cggcaagtac tttaattccc gtaacgatgt ggggatttta ttaaatctaa
     2941 ccttcctgtt cttattgatt cccgtactaa atgaagacat cattttaccc tcccaaacca
     3001 attaagaaat tatataaata tatttatgat ttcaccaaag tgatactatc cgctggtata
     3061 ttccattttt gttatgggct aagtcggtga gcacctccat tccgggtaat cccagttcaa
     3121 acggaactat ttagcggtca actattttat ggttcgggca ccagaacatt gaggctctga
     3181 tgcccgccaa gtcttgcatt aagctatttt acttttgttt tgataagccc caattaaata
     3241 caataccggc aatacaagcc cggttagtat acctaaaaag ttaaatttcc cgccaccggc
     3301 aacctttaaa agatttccgc caaccgatac cgctgctgct agaataccga aaacaatgca
     3361 tgtttgagcc ttttcaggaa ttgctgcatt ctttactccg ataatccccg cgactaggct
     3421 gataatccca ccgatcaata ctagaactga cgatagtact aatagcccca tcttcgcctc
     3481 actgcctaga agagcagcag cggcgcctac accaataacc gcaataattc caaaaataac
     3541 accaaagctc ccaccaataa tcattagaat accggttacc tttaagaatt tgttgctttc
     3601 tcttgccatc tccattcctc cgtatctttt attttttgat tcccacctgt taggcgattt
     3661 ttacagcagg tagctaccat acccatatct tatgggctta acaaaggctg taatatccct
     3721 agggcggtaa tcatgatata gcgccgccac aaaattcgca gcgaccctgc atatccggta
     3781 tcgtcgttgc accacaaaac gaacaggtct gggctgtttt cggagcattg gctgtagcta
     3841 tatcctttct cacaccctcc ctgacctttg taaacgtttc ttttatatcc tctgcggtgg
     3901 cttcacattg ttggtattca gcacttctac gccctacatc cgctccccct tgtataaata
     3961 tacgcccctg gggaggctct atatctatgg cgctgctatt taatttaaaa cgaatctcgg
     4021 aaaaataagg atgattcacg tgaattgtca tccagaaatc gtaatgataa accctgcgcg
     4081 gaggattata gcttacctcg ttaccttggc gatcctttcg cttaagctct gtctggcttt
     4141 catttatctc taagtcgcac cctgtaacct gggaataatc cagtacatca gggttttctt
     4201 cctgccacct acgggaggag gacactataa actttgcttc atcttcatcc aataatatct
     4261 tagttcctac gcctaaagtg cgagtgacat taaaggcccc cacctgggtt ttattcgctt
     4321 cccgataatc catttgtgct ttaatatctg ctacgctgct gcgcctccga tcgctaaaaa
     4381 agggggataa atgcttggca caatccttac aaaggttacc gtcctccaac ttacgatttc
     4441 ccagcattcc gatctttgaa ccgcatatat cacaattttt cctaccgaaa aatgccatga
     4501 gaaaacctcc tagatacaaa atttaagttc ggttaactat atgttttacg acaaatattc
     4561 tacattattg tatat
//
LOCUS       DASRNX010000046        12252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_9324,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000046 DASRNX010000000
VERSION     DASRNX010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12252
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9324"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1197
                     /locus_tag="VFD89_01720"
     CDS             154..1197
                     /locus_tag="VFD89_01720"
                     /inference="COORDINATES: protein motif:HMM:NF025846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZJ56938.1"
                     /translation="MLYYAIYLKSKFLYISDVEIGNYRYPMIFISDGGQELLDSFSIT
                     EEGKLDGLRKESIEIGAAATAMDSDSMILDRFLKNIVKIMTWLKAHTGENITEERIRN
                     VFDIDDQQWDQCAMFFDTLLLGLAKEEWYHPGNTNRIIDRFHKGIRGFLANVPEVQAA
                     AFRAHYEIEDMFYHSQEELIEYYSLQPEDLQESLKNIIKRFFYKKWKRRSWIVKDITN
                     ILFAEEDVYPTDDVDEDGNEDDDDVKVKTAEIDTYTQTLMLYKQGYSISEIAQMRDLK
                     ESTIMGHFAKLIPEHDLNPYEFVDKERVEVIIEVARRVGDESFRAIKGNLPEDYEYGE
                     ISIALALKDDMGL"
     misc_binding    1420..1684
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2036..4567
                     /locus_tag="VFD89_01725"
     CDS             2036..4567
                     /locus_tag="VFD89_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZJ56939.1"
                     /translation="MKLAFSTLGCPDFSWPEIYSMASDFGFDGIEIRGLGKDIFAVEA
                     QPFTEAQLSKTIKKLSELHLEIPCLSSGCCLRFTERAEENHEEIVQYILLASKLKTPF
                     IRILADLEPHPTGEVDDKVVLAALSRLVPIAEENGVTLLVETNGVYADTARLCNLLNE
                     ISSDAVAALWDINHPYHFAGETAEETVQNLGAYIKYVHTKDSVMEDGIVKYRMMGEGE
                     LPMDEIVMALRSINYEGYLSLEWVKRWSSDLAEAGVVFPNYAAFMGQYMERESLKSYL
                     YDNNAGTGKYIWEKDTLIDLTFPQVLDRIVDEFPDQYAFRYTTMDYTRTYEEFRDDVD
                     AFARSLISIGVKHGDKVSIWATNVPEWFIAFWATTKIGAILVTVNTSYKIYEMEYLLR
                     QSDTHTLIMTDGYKDSDYVAIIKELCPELEGAKAGKPLHIRRLPFLRNIITIDSKQKG
                     CLTWEEALGLGEKVGLEEVYRRALSLNRHDVCNMQYTSGTTGFPKGVMLTHYNVINNG
                     KNIGDNMDLSTADRMMIQVPMFHCFGMVLAMTAAMTHGTTMAPLPYFSPRPALKCITQ
                     EKITCFHGVPTMFIALLDHEDFDLADFSHMRTGIMAGSPCPIKVMEEVVDKMNMTEIT
                     IVYGQTEASPGCTQSRVDDSIELRVSTVGRALPSIECKIIDPKTGEDLPDNVDGEFVA
                     RGYNIMKGYYKMPEATAAVIDKDGWLHTGDLARRDENGYYKITGRIKDMIIRGGENIY
                     PKEIEDFIYTHPKVRDVQVIGVPDEQYGEEIMAFVILKDDEELTEDELKNFVRSSMAK
                     HKTPRYIDFVEEFPMNAAGKIMKYKMREQAAERLNLKEKNHIVVV"
     gene            5018..6397
                     /locus_tag="VFD89_01730"
     CDS             5018..6397
                     /locus_tag="VFD89_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HZJ56940.1"
                     /translation="MANKGVRDYRPKLHFTTRKGWINDPNGLVYHDGIYHLFYQHYPE
                     DTKWGPMHWGHAISRELINWEHLPIALAPDELGTIFSGSAVYDRENTSGLAPLGKPPI
                     VAMFTHDGETQQQSIAYSTDGVNFTKYPGNPVIENPGIKDFRDPKVFYNPIWDSWSCV
                     VVAGDRVHFYASKNLIHWTKTGEFGPEGNLCPGIWECPDIFPLRAPNGEEVWVLAVSM
                     TIPLEKGGPKTQYFLGTFDGDTFICTKPFDDIEWIDPGFDNYAAMSYYGDELKDRIIV
                     GWGANWQYAADTPTNEYRGMMTLARKLSLVDTPKGLRLSSTPYAATGEIMTPATEIET
                     EMDIDSEVFGLRIEGEGEFAVTLSNDNGERLVFGVNGDDEIFMDRSMAGDNTFSDIFA
                     SDLFSKVIKKRLYTGTITMEAIFDVSVLEVFADKGTFACAQLVYPKKPYNKICVMGTG
                     VSTHLLRVR"
     gene            6570..7202
                     /locus_tag="VFD89_01735"
     CDS             6570..7202
                     /locus_tag="VFD89_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZJ56941.1"
                     /translation="MRHEAEQACNGTKIMEQFEGRQGNMGIIEKAIIFATEKHYGAVR
                     KGNGIPYIVHPVEALAIAASMTCDPEILAATVLHDVVEDTAVTVDQIEGEFGKRVASL
                     VAWASEDKMPHIPPEKSWKIRKDATIKGLESASLDKKIIILADKLSNMRSLHQGIIRS
                     GDEIWERFNQKDKGMHEWYYRAILERLSDLSESIAYQEFSSLIDRVFKRQ"
     gene            complement(7279..8451)
                     /locus_tag="VFD89_01740"
     CDS             complement(7279..8451)
                     /locus_tag="VFD89_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZJ56942.1"
                     /translation="MENQAQSYRIPLYYLVTSLFWFSLYAFVPNLPIYAESLGASNRL
                     IGLIISSYGFVQMVLRLPLGIYSDKINARKRFILLGILISVISSLGMGFFKTPWSLLV
                     FRGLSGAAAAAWVPFTVLFSSYFTDQQSTKAMGYINAFNNLGQIAALLLGGLVISIFN
                     SAVAPFYLAAAVGALALLLGTGIVENKTEHKPIKLTELLAVGKDARLLIASGLAILSQ
                     INAFATVYGFTPLAAKALGANQGQISLLTTLSTFPGIFAAALSGSNLVVRFGKRRTLT
                     LGFIVMALSAALIPFINSINLLYLSQIAGGFARGAVFSLLMSISIQSVSNDKKATAMG
                     FFQAIYGLGMFVGPIMVGFISDFLNLSWGFWITGLIGIMGAFMTYCLPSLEYRDNN"
     gene            8851..10245
                     /locus_tag="VFD89_01745"
                     /pseudo
     CDS             8851..10245
                     /locus_tag="VFD89_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            10357..10878
                     /locus_tag="VFD89_01750"
     CDS             10357..10878
                     /locus_tag="VFD89_01750"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZJ56943.1"
                     /translation="MLTRKTNPISEESARIYSIMIPDRSDLRQIKEYVDFNFVYDEVK
                     DYYDPYIGRPSEDPVRLIKAELLKIKYNLAGDDELLAEISDRASFRDFLDIDLEEGLW
                     DRTTLVKFRQKVGPKVINKLLANVIELCAEHNMIGGKHEVIDGTNTKARARILGTRDD
                     IYIEKPDDDSKKN"
     gene            10998..11786
                     /locus_tag="VFD89_01755"
     CDS             10998..11786
                     /locus_tag="VFD89_01755"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZJ56944.1"
                     /translation="MVPVGQRISQGDPDARFYKRKNGEKSKLGYQNAFATDIKEGIIT
                     RVITIPGSDNMADRIKPLIEDKVVPELTLDGEFSTGELVSLAQDKGLIYNVPKRVIGE
                     RGVYPKSAFQYDYDLDAYICPENNILSRSSQSGENTIYRGSAKTCHNCPVLGECTKSK
                     SKVRAISRDKYEQAWEVHDEYIQSDQYQFAKVIRGILAEGKFFEANILYGLTIARYVG
                     LILMHAQAQMTAVVINLKRFLKVIKNRKDKANSIPDKSKAPLTA"
BASE COUNT         3872 a         2335 c         2887 g         3158 t
ORIGIN      
        1 gttttttgaa acttcaactg accagttatt catttgatga tacattactt atcctataga
       61 tatataacac caaaccggaa acctaatcat gtttttttat tttcaattga ctgatctgaa
      121 agcgggtttt attaccggaa ttatactgac ataatgcttt attatgccat ttacctaaaa
      181 agtaaattcc tctatatatc cgacgtagag attggcaatt accgttatcc catgatcttt
      241 ataagcgatg ggggccaaga actcctggat tccttttcca tcaccgagga aggaaagctg
      301 gatgggcttc gaaaagaatc tatagagatt ggggcagcgg cgacagccat ggattcggat
      361 tctatgatat tggatagatt tttgaagaac atagttaaaa taatgacctg gctaaaggcg
      421 cataccggag aaaatattac agaggaacga ataagaaacg tctttgatat cgacgatcaa
      481 cagtgggatc aatgtgccat gttctttgac accctgttac tgggactggc aaaagaagaa
      541 tggtatcatc ctgggaatac caataggata attgacagat tccacaaggg aatccgtggt
      601 tttctagcaa atgtaccgga ggtacaggca gctgctttta gggcgcatta cgaaattgag
      661 gatatgtttt atcattccca agaggaactt attgagtatt atagcctgca acccgaggat
      721 ttacaagaaa gcctcaagaa tatcattaag aggtttttct ataagaaatg gaagagaaga
      781 agttggatag taaaggatat cacaaacata ttatttgccg aggaggacgt ctatcccaca
      841 gatgatgtgg atgaggatgg gaatgaggac gatgacgatg tcaaagtcaa aacggcggaa
      901 atagacacct atacccagac cttaatgttg tataaacagg gttatagcat tagcgagata
      961 gcccagatga gggacttaaa agaatcaacc ataatgggcc atttcgccaa attaattccg
     1021 gagcatgatc taaaccccta tgaattcgtg gataaggaaa gagtagaggt gattatcgag
     1081 gtcgcaaggc gggtagggga tgaatcgttt agggctataa aaggaaacct gcccgaggat
     1141 tatgaatatg gcgaaataag tattgcactt gccctaaaag atgatatggg tttgtgacaa
     1201 atagagacaa aaggggacgt acatagatta ccacactaac tgaccccttc ataagatagc
     1261 atggcatggc aactacagat atacaatagg tcaactaaac attttatagt tgcagctatt
     1321 tatctatatt atccacggtt atgcatatat atatgcacat cataggaaaa taccccttga
     1381 aaagacggcc aaaatttgat agacttacta aaaatactac aaaaagccat gatagggaaa
     1441 aagcattgta atccactttc aggaagctgt tgattgctgt gaaacagtag gggttacaaa
     1501 ggcgagctca ccctggagca gtttagccga aagtaagggt tctttttggt cccttattag
     1561 taagcagaac cggatttgtc cgccgttata cggatgtgta ggtgccttat ttggctctac
     1621 cattgagtgg gtatataaat accaatcgga gtggtaccac ggaagtaaag ctttcgtctc
     1681 taacatattg tttaggggtg ggggcttttt ttattcgcag aatttaaggg gataattaca
     1741 cgcctaaagt tcgagaaacc taaatcaggt attataaaac aaggtatcaa taaaaaatac
     1801 atgctatgca acgacggttc cgtatcatta cccagacgca ataaaacata tacggatagc
     1861 ggaggttaca atggtccaaa tggatcactg tcatagtagg atcccttgtt taataaaaac
     1921 tgtgaaacct tattaccggc gttatcgcat attaccggcg ttatcaccta attcattaaa
     1981 cagcgtatta tttgaaattt tcataatatc gtaatcaaaa ctaggaggaa agaccatgaa
     2041 attagcattt tcaaccctgg gttgtccaga tttttcttgg ccggaaatct attccatggc
     2101 cagtgacttt ggatttgacg gcatagaaat cagagggctg ggtaaggata tctttgctgt
     2161 cgaggcacaa ccttttacgg aagcacaact ttctaaaacc ataaaaaagc tgtcagaatt
     2221 acaccttgag attccctgtc tatcatcggg atgttgcctg aggtttaccg agagggcgga
     2281 agaaaaccat gaggaaatag tacaatatat tctcttagcc tccaaactaa agactccatt
     2341 catccgtatt cttgcggacc tggaacccca tcccaccggt gaagtagacg ataaggtagt
     2401 tttagctgcc ctaagtcgcc ttgtacctat agcagaggag aatggtgtaa ccctgctggt
     2461 tgagaccaac ggtgtttatg ccgacaccgc ccgcctttgc aacctgctca atgaaatatc
     2521 cagcgatgca gtggcagccc tatgggatat aaatcatcct taccactttg ccggggaaac
     2581 tgcggaagag actgtacaaa acttgggcgc ctatattaaa tacgtacata ccaaggattc
     2641 ggtaatggaa gatggcatcg tgaagtatcg gatgatgggc gaaggggaac tgcccatgga
     2701 tgagattgtt atggccctgc gttctataaa ctacgagggt tatctatctt tagaatgggt
     2761 aaaacgctgg tcttcagatc tagccgaagc cggggttgtc tttcccaact atgcggcttt
     2821 tatgggccaa tatatggaaa gggaatcttt aaagagctat ctatatgata acaacgccgg
     2881 gaccgggaaa tatatctggg agaaggatac cttaattgat ctaacctttc cccaggtatt
     2941 ggaccgaatc gtggacgaat ttcccgatca atacgcattt cgctatacga ctatggacta
     3001 taccagaact tatgaggagt ttagagacga tgtggacgcc tttgctcgct cactcatttc
     3061 aataggcgtt aagcatgggg ataaggtatc gatttgggcg accaacgtac cggaatggtt
     3121 catcgcattc tgggctacaa ccaagatcgg cgccattttg gtcaccgtca acacctccta
     3181 caaaatttat gagatggaat atctcttaag gcaatcggac acccacacct tgattatgac
     3241 cgatgggtac aaggattccg actatgtcgc aattatcaag gaactctgcc ccgaactgga
     3301 gggggcgaag gcgggaaaac ccctgcatat aaggaggctt ccctttctca gaaatattat
     3361 aaccatagac tccaagcaaa agggttgtct aacatgggaa gaggctctgg gcctgggcga
     3421 aaaagtgggc ttagaagagg tctacagaag ggccctatcc ttaaaccgcc atgatgtttg
     3481 caatatgcag tatacctccg ggactacggg ctttcccaaa ggggtaatgc ttacccatta
     3541 taatgtgata aataacggca aaaacatcgg cgacaatatg gatctttcaa ctgcagaccg
     3601 aatgatgatc caagtgccca tgtttcattg ctttgggatg gttctggcga tgacagcggc
     3661 tatgacccac gggacgacta tggcgcccct gccttatttt tccccgaggc cagcgttaaa
     3721 gtgcattacc caagagaaga tcacctgttt ccatggtgtt cccaccatgt ttattgccct
     3781 gttggatcat gaggattttg atttagcgga cttttctcat atgcgaaccg ggataatggc
     3841 cggaagtcct tgcccgatta aggtcatgga ggaagttgta gataagatga atatgaccga
     3901 gatcaccatc gtctatgggc agaccgaggc ttcccccggc tgcacccaaa gccgagtgga
     3961 cgatagcatt gaacttcggg tcagcaccgt agggcgggct cttcccagta tcgaatgtaa
     4021 aattattgac ccgaaaacag gagaggattt gcctgataat gtggacggcg aattcgtagc
     4081 caggggatac aatataatga aggggtatta taagatgccg gaggctaccg ctgctgttat
     4141 agataaggat ggctggcttc ataccggtga tttggctcgc agggatgaaa acggctacta
     4201 taagattacc gggcgcatca aggatatgat catccgcggg ggagaaaata tttacccaaa
     4261 agagatcgag gactttatct atacccatcc taaggtaagg gatgttcaag taatcggggt
     4321 ccccgacgag cagtatggag aagagatcat ggcctttgta attttaaaag acgatgagga
     4381 actaacggaa gacgagctaa agaattttgt tcgctcttct atggccaaac ataagacccc
     4441 ccgctatatc gattttgtgg aggagtttcc aatgaatgcc gccggcaaga ttatgaagta
     4501 taagatgcgc gagcaggccg ctgaaagact aaatctgaag gaaaagaatc atattgtagt
     4561 ggtttgattg ggggatcctg gaaatatatc atacgataca ttaggtcata gtattgataa
     4621 tttatttttg gggttttatc tgactaccca tttggcgata ctaaagagca tactttttta
     4681 ttggatatgt aaaaggggaa ctgtaattaa acgttgcggt tccccctttt agtgagaagc
     4741 atacagctat tgggaggatt tagaagatag catgtagaat aatgtattat taatcaaatc
     4801 ccttaggggt taatggggga agcacggatt agaggttaaa taggagagta tagggacagg
     4861 aattatggtt gttttgtaag gggtcaatat gaaaaacaat aaaaagcaat agttatcgta
     4921 gttactttgt gaaggaccgg aatgggaaat agcgtttatt gtggtcactt tgcgaaaaag
     4981 gcaacatcga aaataacggt tttggaggga tattaagatg gctaataagg gtgtacgcga
     5041 ttacagaccc aagcttcatt ttactactcg aaaaggttgg atcaacgatc ctaacgggtt
     5101 ggtgtatcat gacgggatct atcatttatt ctaccaacac tatccggaag atacaaagtg
     5161 ggggcccatg cactggggcc atgcaataag cagggagctt ataaattggg agcatttacc
     5221 catcgccctg gcaccggacg agcttgggac tatattctcc gggagcgcag tttatgacag
     5281 ggaaaatact tccggccttg cacctttggg taaaccacct atagtcgcca tgtttaccca
     5341 tgatggtgaa acccagcaac agagcatagc ctatagtacg gacggggtga attttactaa
     5401 atacccgggt aacccagtta tagaaaatcc gggtataaag gattttagag atcctaaggt
     5461 cttttacaat ccgatatggg attcatggag ctgtgtagtg gtagcggggg atcgggttca
     5521 tttttacgca tcgaaaaact taatccactg gaccaagacg ggggagtttg gaccggaagg
     5581 gaatctatgc cccggcatat gggaatgtcc ggacatcttt cccttaagag caccaaacgg
     5641 ggaagaagta tgggttttgg cggtgagtat gactattcct ctagaaaagg gcggtccaaa
     5701 gactcaatat ttccttggca cctttgatgg ggacaccttt atctgcacca agccttttga
     5761 tgacatagaa tggatagatc cgggatttga taactatgca gccatgtcct attatggtga
     5821 tgagcttaag gataggatta ttgttggatg gggagctaac tggcagtatg ctgcggatac
     5881 gccgaccaac gaatatcgtg gtatgatgac cctggccaga aaactttccc ttgttgatac
     5941 tccaaaggga ctgagattat cgtccacacc atatgccgca acgggggaaa taatgacccc
     6001 cgctacagaa atagaaacag agatggatat agatagcgaa gtctttgggc tgcggataga
     6061 aggggaaggt gaatttgccg ttacgctttc aaatgataac ggcgaaagac ttgttttcgg
     6121 tgtcaacggg gacgatgaaa tttttatgga tagaagcatg gcgggggata ataccttcag
     6181 tgatatcttc gcctctgatc ttttcagcaa agtgataaaa aagaggctat atacagggac
     6241 gattactatg gaagccattt tcgatgtctc agtactagaa gtctttgcag ataagggaac
     6301 cttcgcttgc gcacaattgg tctaccccaa aaaaccctat aacaaaattt gtgtcatggg
     6361 gacgggggta tcgacacatt tattgagggt tcgataaaga tatgccatgg ggacgggggt
     6421 atcgacacat tcattggaaa cctgataaga gtgccgggtc aacgatacaa ttatcaggaa
     6481 cacaacgcaa gaagaaggct ggcacatgaa gttgggacag tacgtgaagt tgggatggcg
     6541 catgaagaag gacgacgtat gaaattggga tgaggcatga agcggaacag gcatgcaacg
     6601 ggaccaagat aatggagcag tttgagggga gacaagggaa tatggggatt attgaaaaag
     6661 caattatatt cgctacggaa aaacattacg gtgcagtaag aaaaggaaac ggcatccctt
     6721 atatagttca ccccgtggaa gccctagcaa tagcggcgag catgacctgc gatccggaaa
     6781 ttctggcagc aacggttttg cacgatgtgg tggaagatac agccgttact gtcgaccaga
     6841 tagaggggga atttgggaaa cgggtggcta gcttggtggc ctgggccagc gaggacaaga
     6901 tgccccatat tcctcccgaa aagtcctgga agataagaaa ggatgcgaca attaaaggcc
     6961 tagaatctgc atctcttgat aagaagatca tcatactggc tgataaatta tcaaatatgc
     7021 ggagccttca ccagggtata atccgcagcg gtgatgaaat ctgggaaaga tttaatcaaa
     7081 aggataaagg aatgcacgag tggtattata gggccatttt agaaaggctc agtgacctgt
     7141 cggaaagtat cgcttatcag gaattctctt ctctaattga cagggtgttt aaaagacagt
     7201 aatgcctgtg atgcaatcat aggttctaat cattggacta ttattgcatg gtcttaaatt
     7261 ctgaaagcaa gcaaccgcct aattgttatc cctatattct aaacttggaa ggcaataagt
     7321 catgaaagcg cccattatgc cgataagccc ggtgatccaa aatccccagg atagattcaa
     7381 aaagtcgctg ataaacccga ccataattgg gcctacaaac ataccaaggc catatatggc
     7441 ctgaaagaat cccatagcgg tggctttctt atcgttactg acggattgga tgctgatact
     7501 catcaataat gaaaagacag cacctctagc aaatcccccg gctatttggg acaggtatag
     7561 cagattaata ctattgataa agggtattag tgccgccgac aatgccataa caataaaacc
     7621 cagcgtgagc gtcctacgtt tcccaaatct gacgaccaaa ttcgaaccgc ttagggcggc
     7681 tgcaaatatt ccggggaatg tcgaaagggt tgtcaacagg gatatctgcc cttggttagc
     7741 tcccagggcc tttgctgcca gaggagtaaa tccgtataca gttgcaaagg cattaatctg
     7801 tgataatatc gctaatcccg aagctattag aagccgggcg tcctttccca cagccaaaag
     7861 ttccgttaat ttgataggtt tgtgttctgt tttattttcg actatgccgg ttcctaacaa
     7921 taaagccagg gctcctactg cagctgctaa gtaaaatggg gcaacggcgc tgttaaagat
     7981 gcttatcacc aaaccgccca gaaggagcgc tgcaatttgt cccagattgt tgaaagcgtt
     8041 aatgtaaccc atagccttcg tggattgttg atcagtaaaa tagcttgaaa ataggacggt
     8101 aaagggaacc caggcagcag ctgccgcacc gcttagccct cggaaaacca gtagtgacca
     8161 tggggtcttg aaaaagccca taccaaggct gcttattaca gagattaaga tgcccaataa
     8221 aatgaatctt ttacgtgcgt ttattttgtc cgagtatatg ccaaggggca atctaaggac
     8281 catctgtaca aagccatagg aacttataat aagtccgatt agcctattgg aagccccaag
     8341 actctctgca tagataggca ggttagggac aaaggcataa agggagaacc aaaaaaggct
     8401 ggttactaaa taataaagtg ggattctgta agattgagct tgattttcca tggtgtcctc
     8461 caaaatattg ataattaggt aagttgcaga aatatatcgc atgatatctc cggtcaaaat
     8521 gtagatagtt cagcttttgt tgaaacttca tattaccggt tatttatttg gtgatattaa
     8581 gacaaatttc tactacatca gtataatata tattggatta taacgccata gacatttacg
     8641 aaacgggggt ctcaacttga ctttattacc ggtggattcc atgattcggg ttcatgcctc
     8701 ttcagcgggc tattgttatt cggaatacga aatatttgag attgatgttt tacaacgcaa
     8761 tatatagtaa gggcggaaat aagatgaatg gagggaggat gcatgaaaca caaaagaatg
     8821 aaaggaaaca agggaatggg ggataagagt atattagaag aaggcttata tgagagggtg
     8881 atagataaag aactgggaag tagcctagat aagcttcggg gaatgatata caaagatgca
     8941 cctattgata gcgaggaagc atcaataata ctgtcaaaat acatacagga gatagtcgag
     9001 aagggccttg acaatatcaa agagaagaag ggcaaagaaa agcggagggg tttaaggggc
     9061 cagatcaatt taataaacaa aattattgat ataattgaag gggaaaccgg tgaatcctac
     9121 ttttccaata tgaaaataga cagcagaagt aagcagcttt tagctctata caatatggaa
     9181 aacagtatat ttgcgttaga taagaaaaag gaaataccac gccctgagac atctattgcc
     9241 caaagctcct tatttaccgg ggctattcat gaaccccaaa tgtacaatga attcaaaaaa
     9301 gagatctttt cctgcgataa aatcgatatg ttggtttcct ttataaaatg gagtggccta
     9361 cgtcttatta gggatgagct tttgtcattt acccaaaagg ggggggaatt gcgtgttata
     9421 accactacat atatgggagc aaccgacgca agagccatag aggagctcag agcactacct
     9481 aataccgaaa tcagagtatc ctacgatact aagagcacga gactccatgc taaagcttat
     9541 atctttcata ggaagacagg ctacaccact gcctatgtgg gctcctcaaa cctttccaat
     9601 gcggctatat ccagtggatt ggagtggaat ataaaggtaa cgaaaaaaga tctcccggaa
     9661 acaataaaaa agatagaggc aacttttgat agctattgga actctgcgga ctttgtagag
     9721 tactcaaagg gggattcatc aagactacta agagccctaa acgcagaaaa aaatattaca
     9781 ggggacaatc agcacttttt ccaattcaat attgagccct acccctttca acaggagatc
     9841 ttggataggc tacaagcaga gagggagatc agaggatatt ataaaaacct ggtcgttgcg
     9901 gcgacaggta cgggaaagac agtggtttcg gcactggatt ataaaaggtt tcgcagagag
     9961 caggggaata gcccgaccag attattattt gttgcccatc gcaaggaaat attggaacag
    10021 agcatattat gctttagagg ggtgctcaaa gaccctaact tcggtgagct ttttgtgggt
    10081 agctatgagc ctctaggcat tgagcacctt tttatttcaa tacaaacttt aaattccaga
    10141 gaaatgatag ccaaaacttc tcctgatttt tatgattata tcgttgttga cgaatttcac
    10201 cacgctgctg catcttcata taaagaactg ttatcctatt acaggaggat gtgaagaacc
    10261 cctaagtttc tggctaataa aggtccatcc cccaacattt acatctcatg atcctgatat
    10321 aggtatgcta tagtcataat agaaaggatg atcgttatgt taactagaaa aacaaaccct
    10381 atatcagaag aatcagctag aatatatagt attatgatac ccgatcgctc tgatctacgt
    10441 caaataaaag aatatgtaga tttcaacttc gtatatgatg aagttaaaga ttattatgac
    10501 ccctatattg gtcgtcccag cgaagaccca gtccggctta tcaaagcgga actactaaag
    10561 ataaagtata accttgccgg tgatgatgaa ctcctcgccg aaattagcga tagagccagc
    10621 tttcgggatt ttctcgatat tgatctggaa gaaggtctct gggatcgaac tactttggtt
    10681 aaattcaggc aaaaggtcgg tcctaaggtt atcaataagc tattagctaa tgtaatagaa
    10741 ctctgcgccg aacataatat gataggcggt aagcatgagg ttatcgatgg tactaataca
    10801 aaagccagag ccagaatcct tggaacccgg gatgatatct acattgagaa acccgatgat
    10861 gactcaaaaa aaaactaaaa aacccagaaa ctgatgagta tagagtcaat gttaatcagc
    10921 aggatagctt catcaacctt gagagaacct cttatccaaa gggtgacgct gcaagagaag
    10981 aaagccgtaa acgggagatg gtgcccgttg gtcaaagaat aagccaaggc gatccggatg
    11041 cccggtttta caaacggaaa aacggtgaaa agtcaaagct tggttaccag aatgcctttg
    11101 caacggatat taaagaaggt atcatcacta gggtgattac aatcccgggt tctgataata
    11161 tggctgaccg gataaaacca cttattgaag ataaagttgt tcctgagcta accctagatg
    11221 gcgagttttc aacaggtgaa cttgtttccc ttgcccaaga taaggggtta atctacaatg
    11281 tacccaaaag ggtaattgga gaacgtgggg tataccccaa atcagctttc caatatgatt
    11341 acgatctcga tgcttatata tgccctgaaa acaatatatt aagccgaagc agtcaatcag
    11401 gggaaaacac catttacaga ggttcggcca agacttgcca taactgcccg gtgcttggtg
    11461 agtgtactaa atccaagagc aaagtccgtg ctattagcag ggataagtat gagcaggcat
    11521 gggaagtcca tgatgaatat atccaaagtg accaatatca atttgccaag gtaataaggg
    11581 gcattctagc tgaaggaaag ttttttgaag caaatatact ctatggccta acaattgccc
    11641 gttatgtagg attaatactg atgcatgctc aagcccaaat gacggcagtt gtaattaatc
    11701 taaaacggtt tctaaaagta ataaagaaca ggaaggataa ggcaaattcc attccagaca
    11761 aatccaaggc tccccttacg gcttgaaggg gtaaaaggtg gaaatagagg gtaaacttgc
    11821 atcgtacatt aaccataatg ttgaattaga ggtaatgaaa acctatacat aacccactct
    11881 atatacataa tatgggtgta tatccacgaa catacatggg tatcatttca ttggtggttt
    11941 atgctaatag attttcacat cctcctgtat gtgtattata tatctatgat aattacattg
    12001 tcaatataaa atgagggcac atgattaccc acccagtagt aatcatatac ccccactgac
    12061 aaaataagta ctgatgtagc gccatatgcg gattaattca gcgccttgat taagagccta
    12121 taggacgctt ccatggcatc ttcaatatcc acttcatcgg taaaattcct ttgatgaaag
    12181 tattgcgaga atccaataag agcactggaa atagccatat ttcggtaata ggagttttcc
    12241 acctggtcaa tg
//
LOCUS       DASRNX010000047         7410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_487566,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000047 DASRNX010000000
VERSION     DASRNX010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487566"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1306
                     /locus_tag="VFD89_01760"
     CDS             149..1306
                     /locus_tag="VFD89_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZJ56945.1"
                     /translation="MKRLLHVTLLVLLVTALFVGCNGPAAGNDNEIKIGVNYELSGGV
                     ATYGQSSVDGIELAIKQVNEAGGVDGKKIVLVKYDNKSEPQEATTLANKLIDQDKVVA
                     ILGPATSGSFMSQIPVAEKSKVPVASGSATADEVTVDGSGVKEYAFRICFNDSYQGTG
                     MANYALNNLSKQKAVIIMDNSSDYGKGLAKNFEETFTDGGGTIVTKESFVKGDTDFNA
                     ILTSVKGEDFDVIFLSGYYEEAGLIIKQARNLGIDVPVLGADGFDSPELLELAGADAL
                     NDIYFSNHYSSLDKDPLVAEFIEDFKAEYDREPDAFNALGYDLAKFVVDGIERAGKVD
                     REALKDALAATSDFAGVTGSFSIDENHNPVKAIVVIGLKDGEQHTSEKVSQ"
     gene            1859..2743
                     /locus_tag="VFD89_01765"
     CDS             1859..2743
                     /locus_tag="VFD89_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZJ56946.1"
                     /translation="MEKLLQQIVNGITLGSIYALIALGYTMVYGIIKLINFAHCDIYM
                     IGAYVGYFCMTFFKLGLLPSLLIAMLVCTLLGVLIERIAYRPLRNAARISVLITAIGV
                     SLFLEYGTMFFVKANVRTYPEVTGLLAQRFKIGNIIISMQQVILISITVILMALLQFI
                     VKRTRLGKAMRAVSMDRDAAQLMGIDVNSTVSFTFALGSALAGAAGILVGIYYNTINP
                     LMGVVPGLKAFIAAVFGGIGIIPGAMIGGYFIGAVEVMVAGYGNSMYRDAVVFAILIL
                     ILIIKPSGLLGKNTREKV"
     gene            2747..3703
                     /locus_tag="VFD89_01770"
     CDS             2747..3703
                     /locus_tag="VFD89_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZJ56947.1"
                     /translation="MRKFIALALTYAIVQVLIMTNVISGYFQATLVTVCINIILAVSL
                     NLITGFTGQFSLGHAGFMSVGAYVCALVTMRISTIPGFILGVISGAIAAAILGGLVGL
                     PTLRLRGDYLAIATLGMSEIVRIIFLNMEITNGAAGLNGIPQFINWTWLFIFTAGTVL
                     FLANFVKSAHGRACISIREDEIAAEAMGIDTIRYKVIAFIIGAFCAGIAGAVYSSYFY
                     FLKPDLFGFLKSVDVLVIVVLGGLGSMSGSILAAILLALISTYLQSFPEIRMVLYSLI
                     LVIIMIFRPQGLMGSKEISLSVFNKLGRNKLWNITKSRKGGL"
     gene            3707..4492
                     /locus_tag="VFD89_01775"
     CDS             3707..4492
                     /locus_tag="VFD89_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ56948.1"
                     /translation="MPILSVEKVTKSFGGLAAVSNVSLDLVEGELVGLIGPNGAGKTT
                     LFNLLTGIYMPDTGTISLSVDNERIPLKGLRPHSICQKGLARTFQNIRLFKDLTVLEN
                     VLIAMHQNIKYGLLTSFLHLPAYKREEGKLVEESIELLRIFDLDKKKDEFAKNLPYGE
                     QRHLEIIRALATNPKLLLLDEPAAGMNPAETANLMDVIGWIREKFDLTILLIEHDMTL
                     VMNICERIYVLDYGMVIASGTPEEVRTNKRVIEAYLGEDVGNA"
     gene            4485..5189
                     /locus_tag="VFD89_01780"
     CDS             4485..5189
                     /locus_tag="VFD89_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ56949.1"
                     /translation="MLEIKNMDVHYGMIHALKDVSFEVNQGEVVVLIGANGAGKTTTL
                     RTISGLQRPTGGQITLKGTDITNVSAQQRVKMGISHVPEGRRVFPGMTVLENLELGAY
                     LRRDKAGISEDLDMVYERFPILRDRRRQSAGTLSGGEQQMLAIGRALMSRPEILFLDE
                     PSMGLAPILVQEIFDIIRNINNTGTTILLVEQNANMALQVADRAYVMETGSIVLSGSS
                     SELMSSDEIKRAYLGG"
     gene            5194..5850
                     /locus_tag="VFD89_01785"
     CDS             5194..5850
                     /locus_tag="VFD89_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HZJ56950.1"
                     /translation="MFVRKKMTANPFTISPDKTIPDAQELMTRYDIKRLPVLKNGKLV
                     GVVSKEDILRASPSKATTFSVGEINYLLAKTKISRIMTKDPIIISSNALLEEAATLMR
                     DYGVSFLPVVDDGKLVGIITESDIFDAFIELLGFREKGTRFTIEATDAPGIMSKLTRI
                     MGDFGANISNVAVYRGNAGKSTVVIGINSSNTADMEKTMAEQGFKVLYKLQIKEDGDK
                     "
     gene            complement(5991..7358)
                     /locus_tag="VFD89_01790"
     CDS             complement(5991..7358)
                     /locus_tag="VFD89_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZJ56951.1"
                     /translation="MANNKYSQITPEITNLTKLCYRNSNIDPYLYEKYDVKRGLRDLT
                     GKGVVTGLTEISDIKSSYTDEEGRSIPARGELYYRGIDVGEIVNGSLEKNHFCYEETA
                     YLLLFGQLPTREELEEFIELLAFYRTLPTNFVRDIILKAPSGNMMNTLARSVLTLYAY
                     DEKGDDVSIPNVLRQSLQLIALFPCLSVWGYQAYSHYHGGKSLIIHTPKPELSTAENI
                     LRMLRPDKQYTPLEARILDVALVLHAEHGGGNNSTFTTHVVSSSGTDTYSSVAASLGS
                     LKGPKHGGANIKVVQMFEDIKKSVSDWSDEDEIRRYLTKILDKEAFDKSGLIYGMGHA
                     VYSLSDPRADILKDLVKDLSDEKGLTSEYDLYSSVERIAADLIGQKRRIYKGVSANVD
                     FYSGFVYSMLDLPLELYTPIFAVARIAGWSAHRLEELINSSKIIRPSYINVKDKGAYL
                     PIDER"
BASE COUNT         2008 a         1604 c         1840 g         1958 t
ORIGIN      
        1 gtattcaaaa atgcggtgga gcataacggt aattatataa aaaaaggtga ttgaataatt
       61 ataagggtgg gtatataata atgaataaag gcgctttagc atgctacagc aaagggcttt
      121 tgttttatat gtaggaggag gattatagat gaaaagacta ttacacgtta cattattggt
      181 gttgttagtt acggcacttt tcgtaggctg caacggaccg gcagccggaa acgataacga
      241 aatcaaaatc ggagtcaatt acgagctttc aggtggagta gcaacctatg gacaaagctc
      301 cgtggacggc atcgaattgg ccataaagca ggtaaacgag gcaggcgggg tagacggaaa
      361 gaaaatcgtc ctagtaaaat acgataataa atcggaaccg caagaggcta ctaccctggc
      421 taacaagctg atagaccagg ataaggtcgt tgccatctta gggcctgcaa catccgggtc
      481 ctttatgtcc cagatacccg tagcggaaaa gagcaaggtg ccggtagcat cgggttctgc
      541 cactgctgac gaggttaccg tagacggttc aggggttaaa gagtacgctt tccgcatatg
      601 ctttaacgac tcctatcagg gtaccggtat ggcaaactat gcccttaaca acctttcaaa
      661 gcagaaagcc gtaattataa tggataactc cagcgattac ggtaagggac tggctaaaaa
      721 ctttgaagag acctttactg acggcggggg tactattgtt accaaggaat catttgttaa
      781 aggggataca gactttaacg ccatcttgac cagcgtcaag ggtgaggact ttgacgtcat
      841 cttcctttcc ggatactacg aggaagcggg actcatcata aagcaggctc gcaatctggg
      901 gattgatgtg ccagtcctag gagccgacgg gtttgattct ccggaacttt tagaattggc
      961 cggggccgac gccttaaacg atatctattt ttcaaaccat tattcctcat tggataaaga
     1021 ccccttggtt gcagagttta tcgaggattt taaggctgaa tatgacagag agcccgatgc
     1081 ttttaatgcc ctgggttacg atttggccaa attcgtagta gacggtattg aaagggccgg
     1141 aaaggtggac agagaagcct taaaggacgc attggccgca acttctgatt ttgcgggcgt
     1201 taccggttca ttcagcatcg acgagaacca taatccggta aaggccattg tggttatagg
     1261 tcttaaagat ggggagcagc ataccagcga aaaggtatca caataaccaa tatgtgtatt
     1321 acaagcgtgc ataaagagat atcttaagaa cagttaagcg gggagatact tttccccgct
     1381 tatttacgta atgaagtcat ccgcttttca atagatcatt acctatgaat aagcaatacg
     1441 gaagcatatc ggtaatggct attgaacgct gcttccgaca ttgatttgga gaaaactaag
     1501 gcttattcca ttcgaaaagc ctatcgcttc caaaccggcg tcctgccggt ggaagcggcc
     1561 taaggcgtcc tgccttatgc tacggctttt ccgcactcca taagccaagt tttctaatcc
     1621 aaatcaatga agtcatccgc ttttcaatag atcattaccc atatatgtta tgaaaatacc
     1681 ttggtttaaa ccctaggcgc gctctgatag gtcatgcaat aacaggcctc tttcgcggcg
     1741 tatctatgat ctggaaagaa ttattattaa tctattgtgg aaattagccc aaaaacaagc
     1801 tatacttaaa aggtagactt aatatttcaa actttttggg ggaaagggag gaaagggttt
     1861 ggaaaaatta ttgcaacaga tagttaacgg cataaccctg ggcagtatat atgccctgat
     1921 agcccttggt tataccatgg tatacggcat aattaagcta attaattttg cacattgtga
     1981 tatatatatg ataggggcct atgtagggta tttttgtatg acctttttta agctggggct
     2041 tctgccttct ttgctcattg ccatgttagt atgcaccctg ctgggtgttc ttatagaaag
     2101 gattgcctat aggcctctca gaaatgcggc gagaatttcg gtgctcatta cagcaatagg
     2161 ggtatcccta tttttagaat atggcaccat gttctttgtc aaggccaacg taaggaccta
     2221 tccggaagta actggcctat tggctcagcg atttaagata ggaaatatca ttatctctat
     2281 gcagcaggta atcctcatca gcattaccgt tatattgatg gctcttctgc agttcatagt
     2341 caagaggacc agactcggca aggccatgcg tgcggtctcc atggacagag acgcagccca
     2401 gctcatggga atagatgtaa attctacggt atcctttacc tttgccttag gttcggcact
     2461 ggccggggcc gcgggcatat tggttgggat ctactacaac acgataaatc ccctgatggg
     2521 ggttgttccc ggacttaaag cattcatagc cgcagttttt gggggcatcg gcatcattcc
     2581 cggggcgatg atcggcggct acttcatagg cgcagtagag gtcatggtgg ccgggtatgg
     2641 aaattccatg tacagagatg ccgtggtgtt tgccatactg atactcatcc tcataattaa
     2701 gccctccggg ttgttgggga aaaacacaag ggagaaggtg taagttatga gaaagttcat
     2761 agcccttgct ctcacctacg ccatcgttca agttttgatt atgaccaacg tcatcagcgg
     2821 atattttcag gcgactctgg ttaccgtctg catcaacatc atattggctg tcagtcttaa
     2881 ccttattacc gggttcacgg gacaattctc tttagggcac gccggcttta tgtccgtagg
     2941 ggcgtacgta tgtgccctcg taaccatgag aatatcaacc ataccgggct ttattttagg
     3001 tgtaatttca ggggccatcg cagccgccat cttgggcggg ttggtaggat tgcctaccct
     3061 aaggctccgg ggggattatc tggccattgc aacccttggt atgtcggaga tagttcgaat
     3121 aatatttctg aatatggaaa ttaccaacgg cgcggcggga ctcaacggca ttccacaatt
     3181 tattaactgg acctggctgt ttatctttac ggcggggacc gttctgtttc ttgcaaactt
     3241 cgttaaatcc gcccatggcc gtgcatgcat ctccataagg gaagatgaga ttgccgccga
     3301 agccatgggg atagatacaa ttaggtataa ggtgatcgcc tttatcatag gcgccttttg
     3361 tgcaggtata gcgggggctg tttattcgtc ctatttctac tttctaaaac ccgacttgtt
     3421 cggattttta aaatccgtgg acgtgctagt tattgtagta ctaggagggc ttggtagcat
     3481 gtcgggttcc attctggccg ctattttact ggcccttata tccacctatc tgcagtcatt
     3541 cccggaaata cgcatggtac tgtattccct tatattggtg atcattatga tctttaggcc
     3601 ccaagggctt atgggatcta aggaaatttc cctgtccgta ttcaataagc tgggcaggaa
     3661 taaattatgg aacatcacaa aatcccggaa aggggggctc taggatatgc ctattttgag
     3721 tgtggagaag gtaacaaagt ccttcggcgg attggctgcc gtatccaacg tcagcttaga
     3781 tctcgtagaa ggcgagctag tgggactcat aggccccaac ggcgcaggga agaccacgct
     3841 gtttaacctg ttgacgggaa tatacatgcc ggataccggc accatcagcc tgtccgttga
     3901 taacgaaagg atccccttaa agggattgag accccatagt atttgccaaa aggggctggc
     3961 cagaacattt cagaacatcc gtctgttcaa ggacttgacg gttttagaga atgtgcttat
     4021 cgctatgcat caaaacatta aatatgggct gttgaccagc tttctgcacc tcccggccta
     4081 taagagagaa gaggggaagc tggtagagga aagtatagaa ctccttagga ttttcgattt
     4141 ggataagaaa aaggatgagt ttgcgaaaaa cctgccctac ggtgaacaac ggcacttaga
     4201 gattattagg gcccttgcga caaacccaaa gctgctcttg ctggatgaac ccgcggcggg
     4261 aatgaacccc gcggaaaccg ccaatctcat ggatgtaata ggttggataa gggaaaagtt
     4321 tgatctgaca attcttctta tagaacacga tatgaccctg gtcatgaata tctgcgagcg
     4381 catctatgtt ttagactatg gcatggtaat cgccagcggc acaccggagg aagtcagaac
     4441 caataagagg gtaatagagg cttatctggg ggaggatgtc ggcaatgctt gaaattaaaa
     4501 atatggatgt gcattatgga atgattcacg ccttaaagga tgtttccttt gaggtaaacc
     4561 agggcgaggt cgtagtttta attggtgcca acggagcggg gaagactacc accctgcgca
     4621 ccatatcggg actccaaaga ccgactgggg gtcagataac gctaaagggg acggatatta
     4681 ccaacgtctc agcacagcaa agggtaaaga tgggcatttc ccatgtacct gaaggccgca
     4741 gggtttttcc gggtatgacg gtgctggaga acttagagct gggtgcatat cttcgccgtg
     4801 ataaggcggg aatatcagag gatcttgata tggtatatga gcgcttcccg atccttcggg
     4861 acaggagaag gcaatctgcc ggaaccctgt caggcgggga gcagcagatg ctggccatag
     4921 gccgagccct tatgagccga cctgagatat tgtttttaga cgaaccgtcc atggggctgg
     4981 cacccatatt ggtccaagag atattcgata taatccggaa tataaataat acaggaacca
     5041 caatacttct ggtagagcaa aatgcgaata tggcgcttca ggtagcggac agggcctatg
     5101 tcatggagac cggttctatt gtcctgtcgg gaagcagctc ggaattgatg tccagtgatg
     5161 aaattaaaag agcctattta gggggttgat tgaatgtttg ttaggaaaaa aatgacggca
     5221 aatcctttca ctatctctcc ggacaagacc atacccgacg cccaggaatt gatgacgaga
     5281 tatgatatta aacgccttcc cgtgctgaaa aacggcaagt tggtgggcgt ggtttcaaaa
     5341 gaggatatcc tgagggcatc gccatcgaag gcgaccacct tcagtgtggg tgagatcaac
     5401 tatctcctgg ccaagaccaa gataagccgt attatgacca aggacccgat aatcatatcc
     5461 tccaacgccc tcttagaaga ggcagccacc ctgatgcggg attatggagt cagttttttg
     5521 cccgtagtgg acgatggtaa gctggtaggt ataataacgg agagcgatat ctttgatgcc
     5581 tttattgaac ttctggggtt tcgtgaaaag ggaaccaggt ttaccattga agcgacggat
     5641 gccccgggga ttatgtctaa attgaccaga atcatggggg actttggagc caatatttcc
     5701 aatgtggcag tttacagggg aaatgcgggc aagagcaccg tggttatcgg aatcaattca
     5761 tcaaacaccg cagatatgga aaagactatg gcggagcagg gctttaaggt tctatacaag
     5821 ctgcaaatca aagaggacgg tgacaaataa ggcataaatg cttctgaaat attagacacc
     5881 aaacattaaa tatccaatac taaacatcaa acaccaagta ccaaatatca aataatgcca
     5941 ttggaataaa ttttaaaggc ggtcatagac cgcctgcata atatttttac ttatctttca
     6001 tcgatgggaa ggtatgcccc cttatccttt acgttaatgt aggagggcct tatgatctta
     6061 ctgctgttta tcagctcctc cagcctgtgg gcgctccagc ctgcgatacg ggcaacggca
     6121 aagattgggg tatatagttc caggggcaga tccagcatgc tgtagacaaa accactgtaa
     6181 aaatccacat ttgcacttac acccttatat atcctgcgct tctggcctat gagatccgca
     6241 gctatgcgct ctacgctgga atacagatca tattccgagg ttaaaccctt ctcatcgctg
     6301 agatccttga ccaagtcctt taggatatcg gccctgggat ccgaaaggga atagacagca
     6361 tggcccatac catatataag gccggatttg tcaaaggcct ccttatccaa gattttagtc
     6421 agatacctgc gaatttcatc ctcgtcggac caatccgata cggatttttt tatatcctca
     6481 aacatctgca cgacctttat gttagctccc ccatgcttgg gtcccttcaa agatcctaat
     6541 gaagccgcta ccgaagagta ggtatcggta ccggaagagg atacgacatg ggttgtaaag
     6601 gtagagttat ttccaccgcc gtgctctgca tggagcacca aagccacatc caggatgcga
     6661 gcctcaaggg gagtgtactg tttgtcgggc cgcagcatcc tgagtatatt ctctgcagta
     6721 gacagctcgg gttttggcgt atgtattata agactctttc cgccgtggta gtggctgtat
     6781 gcctgatatc cccagaccga caggcagggg aagagtgcga tcaattggag ggattgcctc
     6841 agtacattgg gtatggaaac atcgtccccc ttttcatcat atgcgtaaag ggtcaacaca
     6901 ctacgagcca gggtgttcat catattcccg ctgggcgcct tcaatataat atccctcaca
     6961 aagttcgtgg ggagggtcct gtaaaaagcc agtaattcaa taaattcctc caactcttcg
     7021 cgggtgggca gctggccaaa caggagaaga taagccgtct cctcgtagca aaaatgattc
     7081 ttttcaaggg atccgtttac tatttccccc acatcgattc cgcgatagta gagttcgccc
     7141 ctcgcgggaa tgctcctccc ctcctcatct gtatagctgg attttatatc gctgatttcc
     7201 gtgagccccg ttacgactcc cttcccggtc aggtccctca ggccccgctt gacgtcatat
     7261 ttttcatata gataagggtc aatattgcta tttctgtagc ataattttgt taaattcgtt
     7321 atttccggtg taatttgtga atacttgtta ttagccatag gcagtctcct ttctttttga
     7381 aggtggaacc ggttgtttaa tctgtaaatc
//
LOCUS       DASRNX010000048        18981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_328475,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000048 DASRNX010000000
VERSION     DASRNX010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 18981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18981
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328475"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..2838)
                     /gene="acnA"
                     /locus_tag="VFD89_01795"
     CDS             complement(121..2838)
                     /gene="acnA"
                     /locus_tag="VFD89_01795"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HZJ56952.1"
                     /translation="MNNKPGMNKRKLSAGGRDYTYYDITNLEQFGIDDTSRLPYSLKI
                     LLESALRNYDDKLVTTEHIRKIASWTGKGNGDEIPFIPSRIILQDFTGVPVVVDLAAM
                     RTAMHEMGADADRINPTIPVDLVIDHSVVVDSYGSNESLEYNVKKELERNRERYELLK
                     WAQGSFANFRVFPPSIGIVHQVNLELLAGVVATRSYGNEEAIFPDTLVGTDSHTPMIN
                     GIGVLGWGVGGIEAEACMLGQPLYFLLPDIVGFKLTGRLSKEVTATDLVLTITDLLRR
                     EGVIGKFVEFFGEGTGNISVEDRATVANMCPEYGATVAYFPVDHKTLKYLRSTGREES
                     QISMVEEYYKTQGMFRTDDSPTPIFTKVLELDLSTVEPSLAGPKRPQDRINLRDMKKS
                     FHSIITAPLEEGGYAVEKEDMDKSVIINYRDGETETLTNGSVVISAITSCTNTSNPYV
                     MMGAALLAKKAVEMGIKKPRHVKSSFGPGSLVVTEYLEDARLLPYLEKLGYNVVGYGC
                     TTCIGNSGPLTEEVSAAIDKNDMVVAAVLSGNRNFEGRIHPQTKMNYLASPPLVIAYG
                     LAGTVNIDLRTEPLSYNDKNQPVYLRDIWPSDEEIESMIEAHVKPSMYREKYENIFHT
                     NELWNSLQVTDGDIYGWSDTSTYIKKPTFFDNMGIRPDKVRDIVGANVLALLGDSVTT
                     DHISPAGSIPEASDAGNHLTSQNVKPDHLNSYGSRRGTHEVMMRGTFANIRIRNRMVP
                     EVEGGFTEHIPTGEVMSIYKAAMRYMKTDTPLVVIAGKEYGTGSSRDWAAKGTALLGV
                     KAVIAQGFERIHRSNLIGMGVLPLQFPEGTSADSLGITGTESFDIIGVTGDLYPNRRL
                     EVRAHHQDGSILTFEVIARIDSRIEAEYYINGGILNLILRSFWESPDRQ"
     gene            3345..4478
                     /gene="mnmA"
                     /locus_tag="VFD89_01800"
     CDS             3345..4478
                     /gene="mnmA"
                     /locus_tag="VFD89_01800"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZJ56953.1"
                     /translation="MKKKVILGMSGGVDSSVAGLLLKEAGYEVIGLFMKNWEEKDENG
                     ECMAALDAEDARRVADQLDIPYYTVNFEKEYWDRVFTYFLKEYERGRTPNPDVMCNQE
                     IKFNAFLDYALKLEGDYIAMGHYAQVEERDGGYYLLRGKDKNKDQSYFLSRIDQKALS
                     KTIFPIGHLEKDEVRRLADEHGFHVADKKDSTGICFIGERNFGQFLDKFLLAEEGDII
                     DVDGGKIGRHSGLIHYTLGQRKGIGIGGIGTGEPWFVVHKDLKNNILYVAQGRNNPAL
                     YSTSLIGESPAWILGHPPNMPINCTAKFRYRQKDIPVTANILEDGNLHIVYDYPVKAV
                     TPGQVAVLYQGEVCLGSCIIGDTEPLDGGNNHRSITPTKNLSP"
     gene            4595..5062
                     /locus_tag="VFD89_01805"
     CDS             4595..5062
                     /locus_tag="VFD89_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZJ56954.1"
                     /translation="MVRCPDDFIGRWFSVLHRSSIGFMTNELKRYGIGSGQIMFLLEL
                     YHCDGINQEELSSFLNIDKANTARALGKLEQEGYLVKKADREDKRAYKIYLTEKALEI
                     KPKIMGVMADWENHLLKELSPEERSFLRKTMKKVGHNCAGNSRCRFCSSPCGE"
     gene            5298..6056
                     /gene="sufC"
                     /locus_tag="VFD89_01810"
     CDS             5298..6056
                     /gene="sufC"
                     /locus_tag="VFD89_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HZJ56955.1"
                     /translation="MERALLSVEDLHVAIGEKLILKGMNLRIDKGEVHVIMGPNGAGK
                     STLAHILMGNPRYTLVRGNMAFEGQNINSLKTDERARLGMFLSFQYPEEISGITLENF
                     LRSSKGSITGKNPSVLSFYKGLKEKMSGLGMGEEYAKRNLNEGFSGGEKKKSEILQML
                     VLEPKLAILDETDSGLDIDAVRIVASGIREYMDGEHSAIIITHHRAILDYVKPDFVHI
                     LLNGRIIESGGAELIEIIENKGFDHLRESAKSGV"
     gene            6059..7474
                     /gene="sufB"
                     /locus_tag="VFD89_01815"
     CDS             6059..7474
                     /gene="sufB"
                     /locus_tag="VFD89_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HZJ56956.1"
                     /translation="MVELERQDNYIDDINRELYDIRDEFSYSYKADKGLTPEIIREIS
                     GKKNEPKWMLDFRLKSLQIYNELDVPPWSPDISDLDIDNIVTYVKPKTDMKISWDDIP
                     DEIKDTFVRLGIPQAEHESLAGVGAQYDSEVVYHSVRQYLLDQGVIYTDMESAVRDYG
                     DIIKKHFMTCVPPRDHKFAALHGAVWSGGSFVYVPKGVKVDIPLQSYFRSNAPGAGQF
                     EHTLIIVDEGAFLHFIEGCSAPKYSVNNLHAGCVELFVREGARLRYSTIENWSKNMYN
                     LNTKRCIVEKNGSIEWISGSFGSRVSMLYPMSVLKGEGASSEFTGVTFAGNGQYLDTG
                     AKVIHGAPYTTSTINAKSIAKDGGVAVYRGIVKIAPRAHHTKSTISCDSLMMDNNSRS
                     DTIPVLDIRNDEVDIGHEAKIGRISDEAIFYLMTRGLSESDARAMIVRGFVEPIAKEL
                     PLEYAVEMNNLIRLELEGSIG"
     gene            7494..8609
                     /gene="sufD"
                     /locus_tag="VFD89_01820"
     CDS             7494..8609
                     /gene="sufD"
                     /locus_tag="VFD89_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HZJ56957.1"
                     /translation="MIKLNELNTLPVPTFRWLRVNELAIDDEIDFEARPYGKDFLQMA
                     PQGELIQRQMPYNGLTDRPIMKDLMDSAGFGVSEDLVGFADTFHNSGMYIEIPRGIKV
                     TEPIMVGYKPDSDNPTVIDNNLIIAHEHSEVTIVMDIASSANASAVHNGVTRVVAKEG
                     AMVTVIKIQRMNDSSFHFDSNLALVGSGARVDYIVAELGSKKAAVNYTCRLSEGAQSH
                     INTIYLGNGDRHLDLSFLMEHQGRRSTSSIEARGALRDMASKTFKGTIDFKKGADKSE
                     GSESEHVLLFDRTVKSISVPLLLCSEDDVKGQHAISSGKVDQDKLFYLMSRGFSRGEA
                     MVLLAEASFSDVIDKIYPKSLEDLIRAEISGRLLHGN"
     gene            8599..9834
                     /locus_tag="VFD89_01825"
     CDS             8599..9834
                     /locus_tag="VFD89_01825"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HZJ56958.1"
                     /translation="MAIEIDRIRRDFKTLGQNVNGKPLVYLDNAATTHKPKSVIDAIG
                     DYYRLSNGNPHRGVHDLGVKSTMLYENARDAIGKFINAKSSSEIIFTKNATESLNLVA
                     YSYGMNYIGEGEEIVLCISEHHSNIVPWQMVAKAKKATLKYLYLGDDYRLKADEIHSK
                     ITEKTRLVSIAKMSNVLGTVYPIDEIVDCAHKMGAVVVVDGAQSLPHYREDVQESDAD
                     FFVFSGHKMFGPMGIGVLYGKEELLNKMPPFLSGGDMIEYVEEQDTSFSDLPNKFEAG
                     TPNVAGALGLATAIEYLDKIGFGEIEEYEEQLTDYALTRLSGVPYIEIYGPADMVDRG
                     PVISFSIEGCHPHDIASIVDLDGIAIRAGHHCAQPLMNYLGAPATSRASLCFYNTKEE
                     IDILIQSLKGLRKWFGYGS"
     gene            9824..10270
                     /locus_tag="VFD89_01830"
     CDS             9824..10270
                     /locus_tag="VFD89_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HZJ56959.1"
                     /translation="MDLNSIYTEIIMEHSQRSHNRRHLENPDWCERGHNPSCGDDITL
                     EVKIAGDKIEEAAFFGSGCAISQASTSIMIDLINGKSVKEANRLAEIFLAMIKKTITD
                     DGELEILEDALALKNISHMPARVKCAVLAWRTLKEAIDERFPGDAD"
     gene            10422..11327
                     /locus_tag="VFD89_01835"
     CDS             10422..11327
                     /locus_tag="VFD89_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZJ56960.1"
                     /translation="MSISWYISGIITIVLWGTADLFYKRGTDPGDRYSHLKIVVMVGL
                     VMGTQAMFELNKMGWRFDFTNIIKYIPVSSMYILSMTIGYVGLRYLEVSINSPVSNTS
                     GAIAGVLAFIVLGKTMNIWQLTAVAMITYGLIAISITQRKLAEEEMGDSEVEANKKYK
                     LGAFALTFPILYAIVDALGTFLDDVYLSKLMSPEEALVSYELTFLFTGILALLYLVII
                     KKEKFMIHREKDKVLAALFETAGQFFYVYALDTNSVVVAPMIASYSIVSVILGRIFLK
                     EKLSYRQYIIIAMVMGGIFLLGLFE"
     gene            complement(11403..11783)
                     /locus_tag="VFD89_01840"
     CDS             complement(11403..11783)
                     /locus_tag="VFD89_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56961.1"
                     /translation="MEVKENIMIPLGYGKFVRSDKIVSLEPIEDDRGPGRRTIVHIED
                     VGSPLIASRTENSILGNMVEIPREELEAAAALELLMDIKDDIMQIGPMLRKSIKKEAQ
                     FDIDNIEKKINEILRHEIEYGDIH"
     gene            complement(11826..12059)
                     /locus_tag="VFD89_01845"
     CDS             complement(11826..12059)
                     /locus_tag="VFD89_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HZJ56962.1"
                     /translation="MIKFATRVDARKYPPREKHDTIFAAFEGLQPGQKMELTNDHDPK
                     PLYYQMMAEYTDQFNWEYLEEGPEVWRVAIGKN"
     gene            12505..12927
                     /gene="msrB"
                     /locus_tag="VFD89_01850"
     CDS             12505..12927
                     /gene="msrB"
                     /locus_tag="VFD89_01850"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007260561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZJ56963.1"
                     /translation="MDDNKKWKEVLSEDAYHILRERGTERPFSGKYNDHYEKGEYHCA
                     ACGNKLFDSETKFDAGCGWPSFYKPSDGDKVGEKRDLSHGMIRTEVICNKCGSHLGHV
                     FPDGPKPTGQRYCINSLALNFKEGKSRGPEAEENGNCQ"
     gene            13041..14255
                     /locus_tag="VFD89_01855"
     CDS             13041..14255
                     /locus_tag="VFD89_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008751866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZJ56964.1"
                     /translation="MKLQGTYNSALVFTDNLEDGAREQIIELLNQDFIAESKVRIMPD
                     VHQGMGCVIGFTADMGDKVIPNIVGVDIGCGMLTIELGNVDIDLPTLDGIINRGIPSG
                     RATHNKRKFRFDRLDDLHCFRDLRNTKRIERSVGTLGGGNHFIELGEDESGSKWLVIH
                     SGSRNLGKQVAEIYQRLAVDLCSGKEEYFIKKEEIITEYKSAGKRKEIKGALDRLAKE
                     YAGLKPRYPKALCYLTGEYRKKYLHDMGICQDYASINREKMAEIILNDLFGKGLKDFN
                     HFETIHNYINFKDNIIRKGAIAAYENERVLIPINMRDGSIIGVGRGNPEWNYSAPHGA
                     GRLMSRTVARRAITMEEFEGSMAGIYSTSINRSTLDEAPLAYKPMEEILENIRETVDV
                     IGIIKPIYNYKA"
     gene            14297..15355
                     /locus_tag="VFD89_01860"
     CDS             14297..15355
                     /locus_tag="VFD89_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HZJ56965.1"
                     /translation="MHSFMNDYSEGAHPRVLELLAKSNYEQNRGYGEDVHSERASGYI
                     RKAIKRDDVDIHFIPGGTMTNLLVISGLLRPHQCVISADTGHINVHETGAIEAAGHKV
                     IAMPSVGGKLSPKSIQAALDYHIGEHMVQPKMVYISNSTELGTVYSKAELIAIHDICL
                     RNDLILFLDGARIGSALTAKANDLSLEDIATLTDVFYIGGTKNGALLGEALVICRDSL
                     KEDFRYLIKQRGGLMAKGFVIGIQFEALFQDGLFLQLAAHANKMAEIVARALEGCGHS
                     FYARPCTNQLFPIMPNHIVEKLSKEFEFSIQEKINEEEAVIRLVTSWATAQESVDRFT
                     ELLSANRAGSKASVLENL"
     gene            15597..16370
                     /locus_tag="VFD89_01865"
     CDS             15597..16370
                     /locus_tag="VFD89_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HZJ56966.1"
                     /translation="MSEFSRTKLLIGTEGINKLRHSRVAVFGIGGVGSFTIEALIRGG
                     VGNITIVDNDEVCPTNINRQLIATNKTIGRPKVEVMGDRILEINPKVKVMMHRTFYTG
                     DNAWEFDLSQYDYIVDAIDTVSSKLVLIENAKACNTPIISCMGAGNKLDPIGFEVADI
                     YKTSVCPLARVMRYELRKRGIDALKVVYSKEPPIRPAKHPAGIPLDIADPSPTKSQER
                     TGKRHVPGSISFVPPVAGLIIAGEVIRDILGIDSQAKFQ"
     gene            complement(16507..17721)
                     /locus_tag="VFD89_01870"
     CDS             complement(16507..17721)
                     /locus_tag="VFD89_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZJ56967.1"
                     /translation="MRLEGKVVIAQGGGPTAVINQSLVGAVLESRKFPQITKVYGSIN
                     GVSGIVNEDFVDLTQETTHNLEEVAITPSSGLFSTREKPDEAYCSEILKVFKAHDVRY
                     FFYIGGNDSSDTVRIVNEHAKQTNYDFRAIHIPKTIDNDLMVNDHTPGYGSAARFVAQ
                     AFMGINLDNRALPGVHIAVIMGRHAGFLTAAASIAQKYPDDGPHLIYVPERAFRVDNF
                     LRDVKNIYDKHGRCIIAVSEGIQDEHGEPIVTKLVEREEDDHGNVQLSGTGALGDLLS
                     QYIKDKLNIQRVRSDTFGYLQRSFMGCVSDVDQLEAREVGEKAAQFAIWHDADGSITI
                     HRTGNYAVDYRLTDLSEVAAKTKLMPDEFINSDGNHVTEAFKNYAVPLLGTNLPSASM
                     IRAPRVPKIIKK"
     gene            18180..>18981
                     /locus_tag="VFD89_01875"
     CDS             18180..>18981
                     /locus_tag="VFD89_01875"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZJ56968.1"
                     /translation="MKRIGFIGVGVMGKSMVLNLMKKGYEVSIYSRTKNKALEVIEKG
                     AVWCDSIRDCVADAEAVITMVGFPKDVEEVYFGDNGILDHARPGSYIIDMTTTSPKLS
                     KRIYQKALEKGVYALDAPVSGGDVGAQKGTLSIMVGGDEEAFGKCHPIFEAMGTNIIY
                     EGPEGAGQHTKMANQIAITGVIASVCEAITYARTVGLDVQKMLDSISAGAAGSWQMSN
                     MAPRIIKDDLDPGFFIKHFIKDMEIAMEEGRDVDLDLEILDNVLKMYKR"
BASE COUNT         5433 a         3805 c         4697 g         5046 t
ORIGIN      
        1 ttaatatact ataatataaa agggaaatat gcaagacagc cggggcttaa acagctctgg
       61 aaaggcccct attggcagga ttagatccct aggttaatgt gtcgaaaaac ctccggggtt
      121 ttattggcgg tcgggggact cccaaaagct tcgcaaaatc aaatttaaga tgccgccgtt
      181 tatataatat tcggcctcga tgcggctgtc tatcctcgcg attacttcaa aggtaaggat
      241 actgccgtct tggtggtggg cgcgcacttc aagcctcctg tttgggtaaa ggtctcctgt
      301 aacgcctata atatcaaaac tttccgtccc tgttattcca agggaatcag cactggttcc
      361 ttcgggaaac tgtaggggta aaacacccat tccgataaga ttgctcctgt gaatcctttc
      421 aaaaccctgg gctataactg ccttaacgcc taacagggca gtccccttgg ccgcccaatc
      481 ccttgagctg cccgtgccgt attctttgcc ggctataacc acgagggggg tatcggtttt
      541 catatatctc atagctgctt tatatatgga catgacctca cccgtaggaa tatgctctgt
      601 aaagcctccc tcaacttcgg gaaccattct gtttcttatc ctgatattgg caaaggtgcc
      661 cctcatcatc acttcatggg ttcccctgcg ggaaccgtag gaatttaaat ggtccggttt
      721 aacattctgg gaggtcagat ggtttcctgc atcagaggcc tcagggatac tcccggcagg
      781 tgagatgtga tcggttgtga cactgtcgcc caataatgcc aatacattag cccctacaat
      841 atccctcacc ttatcgggcc ggatacccat attgtcaaag aaggtaggct tttttatata
      901 tgttgacgta tcgctccacc catagatatc cccgtcggtt acctgtaggc tgttccacag
      961 ctcatttgta tggaagatat tttcgtattt ttcgcggtac atggagggct ttacatgggc
     1021 ctcgatcatg gattcaattt cctcgtcgct tggccatata tccctcaaat atacgggttg
     1081 gtttttgtcg ttataggaga ggggctcagt tcgcagatct atattaactg tgccggccag
     1141 cccataagcg ataactaagg ggggagaagc cagataattc atctttgttt ggggatggat
     1201 acggccttcg aagttgcgat taccgctgag cactgctgcc acgaccatat cgttcttatc
     1261 tatagccgcg cttacctcct ctgttagggg gccgctgtta cctatacagg tggtacatcc
     1321 gtaccccaca acattatagc ccagcttttc cagatagggg agaagtcggg cgtcctctag
     1381 atattctgtg acaaccaatg aaccagggcc aaagctgctc ttcacatgcc tgggcttttt
     1441 aatgcccatc tctacggcct ttttcgccag tagggccgct cccatcataa catagggatt
     1501 cgatgtattt gtgcaactgg taatggctga aattaccacg gacccgttcg taagggtttc
     1561 cgtctccccg tctctatagt taataataac tgatttgtcc atatcctctt tttcaacggc
     1621 gtacccccct tcctccaggg gtgcggttat tatgctatga aaggattttt tcatatccct
     1681 aagatttatc cggtcttggg gtcgtttcgg tccggcaaga cttggttcta ccgtggaaag
     1741 gtcaagctcc agcactttgg tgaatatggg cgtcggagaa tcatccgttc taaacatccc
     1801 ttgagtcttg tagtactctt ccaccatgga tatctgggat tcctcccggc cggtagacct
     1861 taagtatttt agtgttttat gatccacagg gaagtaggcg acggtggccc cgtattcagg
     1921 gcacatatta gcgacagtag ccctatcctc tacgctgata tttcccgtac cttcaccaaa
     1981 gaattccaca aatttgccaa taaccccttc ccttcttaag agatcggtta tggtaaggac
     2041 taggtccgta gcggtaacct cttttgaaag cctacctgtg agcttgaaac cgactatatc
     2101 cggcaataaa aaatagaggg gttgccccag catacacgcc tcggcttcaa tacccccgac
     2161 gccccatccc aataccccta ttccgttgat cattggggtg tgagagtctg ttcccacaag
     2221 ggtatctgga aatatggctt cctcgtttcc ataggatctt gtcgcgacta ccccggctaa
     2281 caactcgagg tttacctgat gcacaatccc gattgaaggg ggaaacaccc gaaagtttgc
     2341 gaaggagccc tgggcccatt ttagaagctc atacctctcc ctgttccttt ctaattcctt
     2401 tttcacgtta tattccaagg attcattgct tccgtaggaa tcaacaacga cggagtggtc
     2461 aataacaaga tcaaccggga ttgtggggtt gatcctgtca gcatctgcgc ccatttcatg
     2521 catggcggtc ctcatggccg ccaaatccac aacaacagga acccccgtga aatcctggag
     2581 gatgatcctg gacgggataa agggaatctc gtccccgtta cccttaccag tccaggatgc
     2641 tatttttctt atgtgctctg tggtcaccag tttgtcgtca taatttctca aggcggattc
     2701 caagagaatt tttagggagt agggcaaccg ggaggtatcg tctataccaa attgttcaag
     2761 gtttgtgata tcatagtatg tatagtccct accgcctgcc gacagcttcc tcttgttcat
     2821 accgggctta ttgttcattt aatcacctca taaagattta aatcacaaaa ttaaatccca
     2881 gagatagtaa tacccaacgg attggattct atcgcttaag gtatcctttg tatgcctctg
     2941 gataccttat acttaaacat tttcgtggat actaaacaag gatagaaccc gcaaaatgtg
     3001 ccctgttatt taaaattgcg gtataggata ccccggagat tctttgtcac ctaacgttaa
     3061 aacccggcag tgtgtattgc gtttccttta ttgttgtagt aaagggtgat ttttgctata
     3121 atgtcttaaa cagacggtgg gtattaatct tccgccccgt tggaacctgt gtgatgcaat
     3181 tgctttcgat gttttaataa atatggttta attgggaaat atggtttatt tgagttaaga
     3241 ggatatatcg gagagtccga aaaatatttt gcgaagtccg gggatatgaa atactatggt
     3301 tggattccaa atccgtagga tcttggaagg aaaaggatgg atctatgaag aaaaaggtaa
     3361 tactgggtat gagtggcggg gtagattctt cggttgccgg tctgttgtta aaggaggccg
     3421 gatatgaagt tataggactc tttatgaaaa attgggaaga aaaggacgaa aatggtgaat
     3481 gcatggcagc tttggatgct gaggatgcca ggcgggtagc cgatcaattg gatatcccgt
     3541 attataccgt aaattttgaa aaggaatatt gggatagggt attcacctac tttttgaaag
     3601 agtatgaaag aggaagaacg cccaatcccg atgtaatgtg taatcaagaa attaagttta
     3661 acgcattttt agactatgca ttaaaattag agggcgatta tatagccatg ggccattatg
     3721 cgcaggtgga ggaaagggat gggggttact atctcctgag ggggaaggat aagaataagg
     3781 accagagtta ttttttatcg agaattgatc agaaggccct ctctaagacc atattcccca
     3841 tcggacatct ggaaaaggac gaggtcagaa ggttggctga tgaacatggt tttcatgtag
     3901 ccgataaaaa ggattccacc gggatatgct ttataggcga aagaaatttt gggcaattcc
     3961 tggataagtt tttgttggcc gaagaagggg atataatcga tgtggacggg ggaaagatag
     4021 gcaggcatag cggccttatc cactataccc taggacaaag aaaaggtatc ggaataggcg
     4081 gaatagggac gggggaacct tggtttgtag tccataagga ccttaaaaac aacatattat
     4141 atgttgccca gggaagaaat aacccggcac tttattctac ttctttaata ggagaatccc
     4201 cggcctggat actgggtcat ccccccaata tgccaattaa ctgtacggca aagtttagat
     4261 accggcaaaa ggatataccc gtcaccgcta atatattgga ggacggtaac ctccatattg
     4321 tttacgatta tccagtgaag gcagtgactc ccgggcaagt ggcggtatta tatcaggggg
     4381 aagtatgctt gggaagctgc ataatcgggg atacagagcc cttagatggg ggaaataatc
     4441 atagatcaat aactccaact aaaaatttat caccatgaag ttgcatagac ccgttacaac
     4501 tatcatgctg ataaatttag tttccgggat gtttatctgt gattttttga gactggtaaa
     4561 atagtagata atgtttaacc gggaggtgta agaggtggta agatgtccgg atgattttat
     4621 tgggagatgg ttttcagttt tgcaccggtc ctccataggc tttatgacta atgaacttaa
     4681 aaggtatggc atcggtagcg gacagataat gttcttgttg gaactgtatc actgtgacgg
     4741 tataaaccag gaagaactgt catccttctt gaatatagat aaggctaata cggctcgggc
     4801 gttaggtaaa ttggaacagg agggttacct cgttaagaag gcggataggg aggataaaag
     4861 ggcctacaag atctatctga ctgaaaaagc cctggagatc aaacctaaga ttatgggggt
     4921 catggctgat tgggaaaatc atctattgaa ggaattatcc ccggaggaga gaagctttct
     4981 aaggaagact atgaaaaagg tcggccataa ttgcgcaggc aatagtcggt gtcgattttg
     5041 ttcgtcgcca tgcggggaat aaattttttt tgatattata gttgcgtacg caactataat
     5101 atgttagtct attggataga taaacaactt caactaaaaa tttatcacca tgaaattgca
     5161 tagacccgtt acaactatca tggcaataaa tttagttttc gtaacagata aatcaaggaa
     5221 aatgttttgc gatatttgga aaataacgta agaagtttat cgtaaaacat atagatgacg
     5281 gtttggagga gttaagtttg gaaagagcat tattatccgt tgaggatcta catgtggcaa
     5341 taggagagaa gctgatactt aagggaatga atttacgaat tgataaggga gaggttcatg
     5401 tgatcatggg ccccaatgga gcgggaaagt ctacactggc acatatactc atgggaaacc
     5461 ccagatatac ccttgtaagg ggtaatatgg catttgaagg acagaatatc aattccttaa
     5521 agacggatga aagggcaagg cttgggatgt ttctttcctt ccaatatccc gaagagatat
     5581 ccggtataac ccttgaaaat ttcctaagat catccaaggg atccataacc ggtaaaaacc
     5641 cctctgttct ttcgttttac aaggggctta aggagaagat gtccgggctg gggatggggg
     5701 aggaatacgc aaagcgtaat ctaaacgaag gattttccgg cggtgagaag aagaagagtg
     5761 aaatacttca gatgctggtc ctcgaaccca aattggcgat actggatgaa acggattcgg
     5821 ggctggatat cgacgcagtt cggatcgttg cctccggaat aagggaatat atggatgggg
     5881 agcattccgc catcatcatt acccatcata gggctatatt ggattatgta aagcccgact
     5941 ttgtacacat cttgctcaat gggaggatta tcgaaagcgg tggggcggag ttgattgaaa
     6001 tcattgaaaa caagggtttt gaccatttaa gagaatctgc aaaatcgggg gtatgataat
     6061 ggtagaactt gaaagacaag ataactacat cgatgatata aatcgggaat tatacgatat
     6121 aagggatgaa tttagttata gctataaggc ggataagggc ctgacccctg aaatcattcg
     6181 agaaatatcc gggaagaaga acgagcctaa atggatgctg gactttcgat taaaatcgct
     6241 acagatatac aatgaactgg atgtaccgcc ctggagcccg gatatttccg atttagacat
     6301 agacaacatt gtaacctacg taaagccaaa gacggatatg aagattagct gggatgatat
     6361 cccagatgag ataaaggata cctttgttag acttgggatc ccccaggcgg agcatgaatc
     6421 ccttgcgggg gtaggggccc aatatgattc cgaggttgtc tatcatagcg ttcgacagta
     6481 tttactggat caaggggtca tctataccga tatggagagt gccgtaaggg attatggtga
     6541 tatcataaag aagcatttta tgacctgtgt accaccgagg gatcataagt ttgccgcact
     6601 ccacggtgca gtatggtccg gggggtcctt cgtatatgtt ccaaaaggtg taaaagtgga
     6661 tatacctctg caatcatact ttagatccaa cgccccgggg gctgggcagt ttgagcatac
     6721 attgataata gtcgatgagg gtgcattttt acacttcata gaagggtgct ctgcacccaa
     6781 atattcggtc aacaacctcc atgcaggatg tgtggaactt tttgttaggg agggagcaag
     6841 gcttcggtat agcacaatag agaactggtc aaagaatatg tataatttga ataccaaacg
     6901 ttgtatcgtg gaaaaaaacg ggtctatcga gtggatttca ggatcctttg gttccagggt
     6961 atccatgctc taccctatga gcgtcctgaa gggggaaggg gccagttccg agttcacggg
     7021 ggtgaccttt gccggaaacg gccaatatct ggatacaggg gccaaggtga tccacggggc
     7081 tccctataca acttcgacaa tcaacgcgaa atccattgcc aaggacgggg gcgtggctgt
     7141 ctacaggggt attgtaaaaa tagctcccag ggcccaccat acaaaatcca ccatatcttg
     7201 tgattcatta atgatggata ataattcgag atccgataca atacctgtct tagatatacg
     7261 aaacgatgag gtggatatag ggcacgaagc caagatagga aggatcagtg atgaagccat
     7321 tttctacctg atgactaggg gccttagcga atccgatgcc agggccatga tagtgcgcgg
     7381 gtttgttgag cccattgcaa aggaattgcc cttagaatat gcggtggaaa tgaataattt
     7441 gatccgattg gaattagagg ggtctatcgg ttgaaggggg aatcgcaata attatgatta
     7501 agttgaatga attaaacaca ttacctgtgc ccaccttcag gtggctcagg gtaaatgaat
     7561 tggctattga tgatgaaatc gattttgaag caagacccta tggcaaagac ttccttcaga
     7621 tggcccccca aggggaactg atacaaaggc aaatgcccta taatggatta acggataggc
     7681 cgatcatgaa ggatttaatg gattctgcag gttttggcgt ttccgaggat cttgtgggat
     7741 ttgcagatac attccataac tccggtatgt atatagagat accccggggt ataaaggtga
     7801 ccgagcctat tatggtcgga tataaaccgg acagcgacaa tccaacggtt atcgataata
     7861 acctcataat agcgcatgag cacagcgaag tcacaatcgt tatggatata gcctcctccg
     7921 ccaatgcatc ggccgtgcac aacggcgtta caagggttgt ggccaaagag ggtgccatgg
     7981 taacggttat aaagatccag aggatgaatg attcttcgtt ccattttgat tctaacctgg
     8041 ctcttgtagg cagcggcgct agggtggatt atatagtagc ggaacttggc agcaaaaagg
     8101 cggcagtcaa ctatacatgt agactcagcg aaggggccca atcccatatc aataccattt
     8161 accttgggaa cggtgacagg catttggatt taagcttttt aatggagcat caggggcgaa
     8221 ggagtacaag cagcatagag gcaagagggg cccttaggga tatggcatct aagacattta
     8281 aggggactat agactttaaa aaaggtgcgg ataagtccga aggtagcgaa tcggagcatg
     8341 tactactatt tgacaggacg gtaaaatcca tttctgtgcc cttgctatta tgttccgagg
     8401 atgatgtcaa gggacagcac gccataagta gcgggaaagt ggatcaggac aaattgtttt
     8461 atttgatgag tcggggtttt agcaggggcg aagctatggt tttgttggcg gaggcatcat
     8521 tttcggatgt aattgataaa atttacccaa aaagcttgga agatctgata agagccgaaa
     8581 tatccgggag gttgctccat ggcaattgaa atagacagga tcagaaggga ttttaaaacc
     8641 ctaggtcaaa acgtcaatgg aaaacccctc gtatacctag acaatgcggc gaccacccat
     8701 aaacccaagt ccgttataga tgctataggg gattattata gattatccaa cggcaatcct
     8761 catagagggg tgcatgatct gggggtcaaa tccaccatgc tctatgaaaa tgcaagggat
     8821 gcgattggga aatttataaa tgcaaaatcc tccagtgaaa taatatttac caaaaatgcc
     8881 accgaatcat taaatcttgt ggcatattcc tatgggatga actatatagg tgaaggcgaa
     8941 gagattgtgc tttgcatttc tgaacaccat agtaatatag tgccatggca aatggttgca
     9001 aaggccaaaa aggcgaccct aaagtacctg tacttgggtg atgattacag attaaaagcg
     9061 gatgagattc acagcaagat cactgaaaaa acaaggcttg tgtcaatcgc aaagatgtcg
     9121 aatgtcttgg gaactgtcta cccgatagat gagattgtgg actgcgccca taaaatgggt
     9181 gctgtggtcg tagtcgacgg agcacaaagc ctcccccatt atagggagga tgtccaagaa
     9241 tccgatgcag actttttcgt attttctgga cataagatgt ttggacccat gggcatcggc
     9301 gtcctatatg gcaaggagga gttgctcaac aaaatgccgc cctttctctc aggtggggat
     9361 atgatagagt atgtagaaga acaggataca agcttttcgg acctgcccaa taaatttgaa
     9421 gcggggaccc ccaacgtggc aggcgccctg ggtttggcca ctgctattga atatctggat
     9481 aaaataggct ttggggaaat agaagagtat gaagagcaac taacggatta tgccctaact
     9541 aggctctctg gggttcccta tattgaaatc tacgggcctg cggatatggt ggataggggc
     9601 ccggttattt cctttagcat cgaaggttgt catccccatg atatagccag tatagtggac
     9661 ttggacggta tcgctataag ggccgggcac cactgcgccc aacctttgat gaactattta
     9721 ggggcccccg ctacatcaag ggccagcctt tgcttctata atacaaaaga agagattgat
     9781 atattaattc aaagccttaa gggcttaagg aagtggtttg gttatggatc ttaattcaat
     9841 ttatactgaa attataatgg aacacagtca gcgcagccat aacaggagac atttggaaaa
     9901 tcccgactgg tgtgaaaggg ggcataaccc aagctgcggg gatgatatta ccctagaggt
     9961 taagattgcc ggagataaga tcgaagaggc cgcatttttt ggaagcggat gcgctatatc
    10021 ccaggcctcg acctctatta tgatagactt aatcaacggt aaatctgtaa aagaggctaa
    10081 taggttggca gagatctttc tagccatgat aaagaagaca attaccgacg atggggaatt
    10141 ggagatccta gaggacgcct tggcccttaa gaatatatcc catatgccgg ccagggtaaa
    10201 gtgtgcagtc ctggcatggc ggaccttgaa ggaagccatt gatgaaaggt tcccgggaga
    10261 tgcggattag acaaggcaca aggcctccaa acccgccggg aaagcgccgg tgccatgggt
    10321 cggttaacaa aaaaagcaga cagattcccc tttttgttct ataatggact ggtgtgcgaa
    10381 aaattagcgt tgcagatcaa ggagaatata ggggggaaga tatgtctatt tcatggtata
    10441 tatccggaat aataacaata gtgctttggg gaaccgccga tctcttctat aagaggggca
    10501 ccgatccggg ggataggtat agtcacctaa agatagtagt gatggtggga ctggttatgg
    10561 gaacacaggc catgtttgag ctaaataaga tgggttggcg atttgatttt acaaacatca
    10621 taaaatatat cccggtgtcg tccatgtaca tcctatccat gaccatcggc tatgtagggc
    10681 tcagatatct tgaagtatcc attaattcgc cggttagcaa tacctcaggt gctatagccg
    10741 gggtactggc cttcattgtc ctagggaaga ctatgaacat ctggcagctg acggcggtag
    10801 ctatgatcac ctacggcctt attgcaatct caatcaccca gagaaagctg gccgaggaag
    10861 agatgggaga tagtgaagtt gaggccaata aaaagtataa gttaggggcg tttgctttaa
    10921 cctttcccat actatatgcg attgtggatg cgctgggtac cttccttgac gatgtctatc
    10981 ttagtaaact aatgagtccc gaagaggcat tggtatccta tgagctgacc ttccttttta
    11041 ccgggatttt ggctttactc tatttggtga ttataaagaa ggagaagttt atgattcata
    11101 gggagaagga taaggtcttg gctgctctgt tcgaaactgc gggtcaattc ttctatgtct
    11161 atgccttgga tacaaactcc gttgtcgtag cgcctatgat cgcctcctac agcatcgtct
    11221 ccgttattct aggcagaatt ttcctcaagg aaaagctatc gtataggcaa tacataataa
    11281 tagctatggt catgggaggt atttttttac tagggttatt cgagtagagc atgtggtagt
    11341 acaattcatg tttgaagaca atatccccac aacatcatag agcaatccca ttgctgggaa
    11401 tatcaatgga tatctccgta ttctatttca tgcctcagta tttcatttat ctttttttct
    11461 atattgtcta tgtcaaactg tgcctctttc ttgatgctct tacgcagcat cggacctatc
    11521 tgcattatat cgtctttaat atccatgagt agttccaaag ctgctgcagc ctccagttct
    11581 tccctgggga tttccaccat atttcctagg attgaatttt cggttctcga ggctataagg
    11641 ggcgacccca catcctcaat atggactatg gtacgccttc cggggcctct gtcatcttct
    11701 atgggttcaa ggctgacgat tttatccgag cgtacaaact tcccgtatcc caaggggatc
    11761 ataatatttt cctttacttc catactcctc acctccatat gggcctaaac taaatgttca
    11821 tagaattagt ttttaccaat ggctactcgc catacctcag gaccttcctc caaatactcc
    11881 caattaaact ggtcggtata ttccgccatc atctggtaat ataggggttt tggatcgtgg
    11941 tcgttagtta attccatctt ttgacccggc tgtaatcctt caaacgccgc aaaaatggtg
    12001 tcatgctttt ccctaggggg atattttcgg gcatctaccc gggtagcaaa ctttatcatg
    12061 caatcagctc ctacattgtc tttattttcg ttaaacatcg gaaacaattg cattggcaat
    12121 tgcaccacac aggtttcaac gaggcgggtg atttataccc atcgcctgtt ttactaatgg
    12181 gtactcgccg cttataggag cgggggacat cttgtcgact cgttatatcc tgatacccgc
    12241 tattctttac agcccggcgg gggcagcact tgatcctttt agttagtatt ttcaattgtg
    12301 ttttatctat gctgtctgtg gggtattata tctatagggg tgaataatta ttctataggc
    12361 tagtgagcta taaccttccg aattaactgt tcaaggggta gaccaattaa caagactttt
    12421 ctatgaataa tatgaaatct caaggatgag aggaatatat agaaacaatc aaagattata
    12481 gaggaaggga gtgaagatac ggaaatggac gacaacaaaa agtggaaaga agtgctatcc
    12541 gaagatgcat atcacatatt gagggaaagg gggacggaga ggcccttttc tggtaaatat
    12601 aacgaccact atgagaaggg tgaataccat tgtgccgcct gcggcaataa actattcgat
    12661 tccgaaacta aatttgatgc tggatgtggc tggccaagct tttacaaacc ttcagacggg
    12721 gataaggttg gcgaaaaaag ggatttgagt catggtatga tccgaacgga ggtgatatgc
    12781 aataaatgtg ggagtcatct gggacatgtt tttcccgatg gccctaaacc caccgggcaa
    12841 agatattgca tcaattcctt agcattgaat tttaaggagg ggaaatcccg gggtcctgag
    12901 gctgaagaaa atggaaattg tcagtaaaag aagatcaata caagaaggga tagaatccca
    12961 taaataaaag atttgtgagg gaggggaatt gttaccgggg agcatggtat ttagcagagc
    13021 ttttcggtag ctttgaatag atgaaattac agggaacata caatagtgca ttggtgttta
    13081 cggataatct agaggatggg gcaagggagc agataattga attgctaaac caggatttta
    13141 tagccgagtc taaggtaagg ataatgccgg acgttcacca aggtatgggc tgcgttatcg
    13201 gattcaccgc tgatatgggg gacaaggtga ttcccaatat cgttggggtt gatatcggat
    13261 gcggtatgct caccatagaa cttgggaatg tggatatcga tttaccaact ttagatggta
    13321 taatcaatag gggaataccc tcggggaggg cgacccataa caaaagaaaa tttagattcg
    13381 acaggctaga tgatcttcat tgctttaggg atttgaggaa tacaaagaga atagaaagaa
    13441 gcgttgggac cttaggggga ggaaaccatt ttatcgaact gggcgaggat gaaagcggaa
    13501 gcaaatggct ggtaatccat tcgggaagca ggaacctagg gaagcaagtg gcggagatct
    13561 accagcggtt ggcagtggac ctttgcagcg gcaaagagga atattttatt aaaaaggaag
    13621 agataatcac cgaatataaa tcggcaggca agaggaagga aataaaaggc gctttggatc
    13681 gtcttgcaaa agaatatgcc ggccttaagc ccagataccc aaaagcctta tgttacttga
    13741 ccggggaata cagaaaaaaa tatctacacg atatggggat ctgccaggat tatgcgagta
    13801 ttaataggga aaagatggct gagataatac ttaacgattt attcggtaag ggccttaagg
    13861 actttaacca cttcgagaca atccataatt atattaactt taaggataac atcatccgga
    13921 agggagcaat tgcggcctat gagaatgaaa gggtcctaat acccattaat atgagggacg
    13981 gctccataat tggggtaggc aggggtaatc ctgagtggaa ctattcagct ccccacggcg
    14041 caggaaggct catgagcaga acagttgcca gaagggccat aaccatggag gaatttgaag
    14101 gaagtatggc ggggatctat tccacgtcca taaacaggtc caccctagat gaggccccac
    14161 tcgcatacaa accaatggaa gaaattttgg aaaatatccg agaaacagta gatgttatag
    14221 gcattattaa accgatttat aactataagg cataggcata gaggtactac ggagcaaggg
    14281 atttggagga tgtgtgatgc acagttttat gaacgattac agcgaaggag cccaccctag
    14341 ggtgctggaa ttgctggcta aatcaaatta tgagcagaat aggggatacg gtgaggatgt
    14401 ccacagcgaa agagccagtg gatatataag aaaggcgatt aagagggatg atgtggatat
    14461 acattttatt cccggaggca ccatgaccaa tctattggtc atctccggcc tattgaggcc
    14521 ccatcaatgt gttatatccg ccgataccgg gcatatcaat gtccacgaga cgggggctat
    14581 tgaagctgcg gggcataaag taatagcaat gccgtctgtg gggggcaagc tcagtcctaa
    14641 atcaattcag gcggctttgg attatcatat aggcgaacat atggtgcagc caaagatggt
    14701 gtatatatcc aattccacgg agctggggac tgtttattcc aaggctgaac tgattgccat
    14761 acatgatatc tgcctgcgaa acgatttaat tctttttctg gacggcgctc gaatcggttc
    14821 ggccctgacg gcaaaggcta acgacttatc cctagaagat atcgcgacgt tgacggacgt
    14881 cttctatatt gggggcacaa agaacggtgc cctcctaggt gaggccctag taatctgcag
    14941 ggatagttta aaggaagact ttcgatattt aataaagcaa aggggaggcc taatggcaaa
    15001 aggctttgtt atagggatcc aatttgaggc attgtttcaa gacgggttgt tccttcagct
    15061 ggctgcccat gccaacaaga tggcagaaat agtggcaagg gcactggaag ggtgcggcca
    15121 ttcgttctat gcgaggccgt gtaccaatca actatttccc attatgccaa atcatatcgt
    15181 tgaaaagctg tcgaaagaat ttgaattctc catccaagag aaaatcaacg aagaagaagc
    15241 tgtgatccgc ttggtcacat cctgggcaac cgcccaagaa agcgtagacc gctttacaga
    15301 gcttttatcc gccaataggg ccggttctaa agcctccgtt ttagaaaatc tataatctcc
    15361 ccatgcagtt tatggttcta gaagggggtc acatggggag catcttctat ccgtatcatc
    15421 tctacatagt ttccatcgcc cattagtgtc tttgggttga aaggattagt aaagcagagt
    15481 ttttatttcc tggacacatg cattataatg agtataatat attgtgcaat tgtcgcagcg
    15541 atcgctgcga tgctctgtaa tgggttattt gatagaatca tttgggggag aacgagatga
    15601 gcgaattttc caggactaaa ctcttgattg gaacagaggg catcaataag ttaagacact
    15661 ccagggtagc ggtatttggt ataggtgggg tcgggtcctt tactatagag gcgttgatca
    15721 ggggtggggt gggcaatatc acaatagtcg ataacgacga ggtatgtccc acaaatatta
    15781 accggcaact gatagctacc aataaaacca tagggagacc caaggtcgag gtcatgggtg
    15841 acagaatttt ggagataaac ccaaaggtca aggtgatgat gcaccggacc ttttatactg
    15901 gcgacaatgc atgggaattt gacctgtctc aatatgatta cattgtggat gccattgaca
    15961 cagtgtcatc caaactggtc ctaattgaaa atgccaaggc ctgtaatact ccaattatta
    16021 gctgcatggg ggcaggcaac aaattggatc ccattgggtt tgaggtagcc gatatataca
    16081 agaccagcgt atgtccactg gccagggtca tgcgatacga attgagaaaa cgaggcatcg
    16141 atgcgttaaa ggtggtctac tctaaagagc ccccaatcag gcctgccaaa catcctgcgg
    16201 gtatcccatt ggatatagcg gatccctccc cgacaaaatc acaggagcga accggtaaaa
    16261 ggcatgtacc tgggagcatc tccttcgtac cccccgtggc agggcttatc atagccggcg
    16321 aggtgattag ggatatattg gggattgact cccaggccaa attccaatag gaacaggcca
    16381 tctaaagcgg ggaatcgtag cggcatatat tatatatgaa gtaccttgct ggttcatatt
    16441 atggatgaat tgcaggcatt attaataaat ctataaaaaa aggcgggaaa ctattcccgc
    16501 cacattctac tttttgatta tcttcggtac tctaggggcc cttatcatgc tggctgaggg
    16561 caagttggtc cctaatagcg gaactgcata gtttttaaag gcttcagtga catgatttcc
    16621 atcgctgttg ataaattcat cgggcattaa cttggtctta gcagctactt cgctaaggtc
    16681 ggtgagccta taatccacag cgtaattgcc tgtcctatga atggttatgg acccatcggc
    16741 atcgtgccat attgcgaact gggcagcctt ttcaccaact tcacgcgctt ccaattggtc
    16801 gacatcggat acacatccca taaaggatct ctgtaggtat ccaaaggtgt cggatcttac
    16861 ccgctggata ttcagtttat cctttatata ttgagagagc aggtcaccta aagccccggt
    16921 accagatagc tggacattac cgtggtcatc ctcttcacgc tctaccaatt tggtgactat
    16981 gggttcgcca tgttcatctt gaatcccttc agaaaccgct ataatgcacc ttccatgctt
    17041 gtcatagata ttctttacgt cccttaggaa gttatcgact ctaaaggccc tttcgggaac
    17101 atatattaaa tgcggtccat catccggata tttttgtgca atggaagctg cagccgtcaa
    17161 gaaaccggcg tggcgtccca taataacggc tatatggact ccaggcaaag ccctgttatc
    17221 cagatttata cccataaacg cctgggccac gaatcttgcg gcggaaccat atcccggtgt
    17281 atggtcattg accatcaaat cgttatctat ggtttttgga atgtgaatag ccctaaagtc
    17341 ataatttgtc tgcttggcat gctcattgac gattcttacg gtgtcagatg agtcattacc
    17401 tcctatgtag aaaaagtacc tgacatcatg ggccttaaat acctttaaga tctcactgca
    17461 ataggcttca tcgggtttct ccctggttga aaacaaccct gaggaggggg ttatagccac
    17521 ttcttctaaa ttgtgggtag tctcttgggt taagtcaaca aaatcctcgt taactatgcc
    17581 ggaaacaccg ttgatcgatc catacacctt tgtaatctgc ggaaactttc ttgattcaag
    17641 tacggccccg actagggact gattaattac cgcggtcggg ccaccgcctt gagctataac
    17701 aactttacct tccagtctca ttcgttactt ctctccttat tctaaagatt ttccaataca
    17761 cctatattgt atcagattta taatataggg caaagaatat tttgtcaaac tattgacagg
    17821 tatggggcaa ataatgtaaa taaaaagatg cgattaaaac atcggaagca attgcattgg
    17881 caattgcaac acacaggttt caacgaggcg agggatttat acccaccgcc tgttttacta
    17941 atcggaactc gccgcttata gaagcgggag acatcttgcc gactcgttat agtcatgaag
    18001 aatatttacc attatattat accgaatttg gtgttgcgat aaactaaaaa taagagatgc
    18061 ctgataactt accttaagaa aacattccct ttagaccaag tggagtttgc cataggggaa
    18121 agcttttggt aagataaaat tgtgaagacc agaaaaatag atagaattgg ggtgtactta
    18181 tgaagagaat tggatttata ggtgttggag tcatgggaaa gtccatggtg cttaacctaa
    18241 tgaaaaaagg ttacgaggtg tctatctatt cccgtacaaa gaacaaggcg ttggaggtta
    18301 tagaaaaggg agctgtatgg tgcgattcca tcagggattg tgtcgctgac gcagaagccg
    18361 ttattacaat ggttgggttc ccaaaagatg tggaagaggt atattttggg gataacggta
    18421 ttctggacca tgcaaggccc ggcagctata ttatagatat gacaacaacc agccctaaac
    18481 tcagcaaaag gatataccag aaggcactgg aaaagggagt atacgctttg gatgcccccg
    18541 tttccggcgg ggatgtaggc gcccaaaagg gaaccctatc cataatggta gggggcgatg
    18601 aagaagcctt cggaaaatgc catccaatct ttgaagctat gggaacaaac atcatctacg
    18661 aggggcccga aggggcgggt cagcatacca agatggccaa tcaaatagcc attacagggg
    18721 ttatagccag cgtgtgtgag gccattacct atgcgaggac cgtcgggttg gatgtccaga
    18781 aaatgctgga tagcataagc gctggggctg ccggaagctg gcagatgtcg aatatggcgc
    18841 ctcgaataat aaaggatgac ctggacccag gatttttcat caaacacttt ataaaggata
    18901 tggaaattgc catggaagag ggtcgggacg tagatctgga tcttgaaatt ctagacaatg
    18961 tgctaaagat gtacaaaaga c
//
LOCUS       DASRNX010000049         7921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_434142,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000049 DASRNX010000000
VERSION     DASRNX010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434142"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VFD89_01880"
     CDS             <1..1081
                     /locus_tag="VFD89_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ56969.1"
                     /translation="VIKMELSLKPDFDKTQERIDAFWNCEIVDRPPVSIILPKENPKP
                     LPDKKYKNHRERWLDIEFRAELIDRELENNIYLADGVPTVFPNMGPEIFSAWCGCPYQ
                     FGETTTWSEPCILDWGTDAQGAAFDPEHPLFKVMVEFTELLIERGKDKFIVGLTDFHP
                     GGDHIAALRDPASLAMDMIENLDFVVQKLKESYKDYYHVYDIFYNMLRDAGMPITSWT
                     PLFYEGRYYIPSNDFSCMISKKMFDEVFVPGIIEECNFYDRSIYHLDGPGALRHLDSI
                     LAIENLNAVQWICGAGNEGYAKWIDVYQRIQDGGKGLQLMITLDELPLVFETLRPEGV
                     WFSNIYGIDDIVTAERVVKRIEAWK"
     gene            1158..2309
                     /locus_tag="VFD89_01885"
     CDS             1158..2309
                     /locus_tag="VFD89_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HZJ56970.1"
                     /translation="MKILHIADLHIGKRVNGFSMIEDQEHILDEILRITREEEPGAVL
                     MAGDIYDKSQPSAEAVELLDEFLTRLTSLGQPVFIISGNHDSPERLSFGSRIMQSNGL
                     HIAGVFDGSLEKMTLEDEHGIINIYMLPFIKPAMVRPFFEEPIESYEEAVKTVISASS
                     VNADERNILVAHQFVISGMKQPQQSDSEAISVGGLDHVDASVFDDFDYVALGHLHGPQ
                     PMGQDWIRYAGSPLKYSFSEARHTKSMTMVELGGKGEISIEQIPLAPLRDLRQIKGPI
                     KELISVGSEDAEGAMDYIHAILTDEEEIYDAIGQLRQVYPNLMALDLENSRTQQVYDS
                     EVLAASDSAEKDPLDLFSEFYQIQNNNDLTREEIRVMKEIFEKAGGVGI"
     gene            2306..5449
                     /locus_tag="VFD89_01890"
     CDS             2306..5449
                     /locus_tag="VFD89_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HZJ56971.1"
                     /translation="MKPIKLVMSAFCSYPDRVELDMTAFKGRGLFLITGDTGSGKTTI
                     FDAMAFALFGEASGSTRGVETLRSDFAKPDTETYVEFTFLHRDKQYTVTRNPRYERPK
                     RSGQGLTTENSNATLELPSGDIVTGLNNVTAKIESILSINCRQFKQIAMIAQGEFLQL
                     LLSSTKDRGEIFRRVFNTDLYQTAQRLLKEGEREAKKRCERVELSILQYISEISASES
                     ERGRELSLKMGTANIHTAGDIYIGLQGLILEDTIKRNDLKKQSDELEQRLAGQIARIT
                     EGQYINQSFEDLKAVVKREKVLTESLKRHNREKKDLEMAEKALYTISPLEIAYRRERK
                     VREGLIENITKLTQIIEGQEKTLGLASESYNIERKKEPDRESLTSAIDSLKKMVPAYE
                     NAQTLEKDLRALSARHKGLTEELEKLKLQKDGLMDNKKALGTELEQLGDIEIRTSNCQ
                     QDLRQLETVLAQLVELQNYLAQYNKLEVESHKLKKQFQKAQDKYTIVNKDYLEKETAF
                     FREQAGIMAAGLKEGEPCPVCGSRAHPNKAIPASDAPSEAELKRLKEKTDLARGDMEE
                     ASNKSATKLTEIKLAKEQLLKASKVHFLKLDEEPWEGVLESLIESELEQNNEKKKGLD
                     IKYLKLKDEVTRKSEAAKQLKLLEESLQNCEEQMNKVEEERNNVNTDLASKEGELKNL
                     KSSLEYENRQRAEMTIKEWEVRLADLKAAFDKAEQAYHKAKNELNNSQTLLKDHRERL
                     NETEETKKQAQIAYTEGIIRSGFKDESDYQEALRTEPEIRELRGSIDEYIREVRSVDQ
                     DIRRLREETKDKEEQDLEGLKAIKKNLEEAKSKVGESAQGLVTRLEANKSILKALEKA
                     IPKAISYQKEYLLLGNLSRTANGELSGKQKLAFEQYVQASYFNRILIESNKRLRLMTN
                     NRFELLRKEDATDLRSRGLEIDVLDHYTGRVRSVKSLSGGESFKASLSLALGLSDVIQ
                     SHAGGVEIDTLFIDEGFGALDAESLEQAIQTLAGLAEGNRLVGIISHVSELKERIDRQ
                     ILIKKTPKGSALSITGA"
     gene            complement(5571..7148)
                     /locus_tag="VFD89_01895"
     CDS             complement(5571..7148)
                     /locus_tag="VFD89_01895"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZJ56972.1"
                     /translation="MSLHKLFQSVITMSLMGSVVAGIIMLIKALLRDKSNAAFHYYIW
                     FLLMVRLIIPYAPETSFSIFNLVPQVFENIERYTRPQFDVGHNLNLSSNADVEQPTQR
                     EDDSGQILHEGPGDKSDTIHNRNDYFKAQSWINVVKGIWATGVLAIILYNLGIYSILL
                     YKINNMPECVEDNMAEILERLRKKTKTKRLPRIVYSPQATAPSLFGYFKPKILISHQL
                     ANGLSPEELEYVFLHEMVHLRRGDILINWIMVTIQALHWFNPVVWYAFRKMRSDCEIA
                     CDAHVLAYLEPENRKDYGNTIISLLAILSKPYWNPGTVGLATNKSELKRRIIMIRKFN
                     TIPFRWTIIAAATIILIGGIGLTNGLTRAKGLGKPPLSTKDKDDVALIRENQMKENPD
                     SAVENVESSKMDEVHEVVDTDNSFPQKPQVSVEIDMEIAERDQKQVDEGHSPWQLSPF
                     AVAQTFVGIQISPDGIQGDFPVDTEDMKIAYETNEVTIIEISGDKTPIAKVYLKRLVK
                     SNDDGIWSVVGYDPVKK"
     gene            complement(7177..7539)
                     /locus_tag="VFD89_01900"
     CDS             complement(7177..7539)
                     /locus_tag="VFD89_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HZJ56973.1"
                     /translation="MRELPKISDAEWQVMKVLWKQSPLNASQVINELQNYTTWNPKTI
                     HTLLGRLVDKGALNVDKGVTPYAYYPRVSQEECMREETKFFLEKIFDGSMKRLLVSFV
                     ESEELSREEIEELRRILD"
BASE COUNT         2361 a         1664 c         1887 g         2009 t
ORIGIN      
        1 ggttataaag atggaattat cccttaagcc ggattttgac aagacccaag aaagaatcga
       61 tgcattttgg aattgtgaga tagtggacag gcctcctgtc agcattatcc ttcccaaaga
      121 aaatcccaaa cctctgccgg acaagaaata taaaaaccat agggaaagat ggctggatat
      181 agagtttagg gccgaattaa tagatcggga actggaaaac aatatctacc ttgcggacgg
      241 ggtgcccacg gttttcccca atatgggtcc tgaaatcttt agtgcgtggt gcggctgtcc
      301 ataccaattc ggcgagacga ctacatggag cgaaccctgc atcttagatt gggggacaga
      361 cgcccaaggt gcagcctttg acccagagca tcccctattt aaggttatgg tggaattcac
      421 agagctgttg atagagaggg gtaaggacaa attcattgtg gggctcacgg attttcatcc
      481 cggcggcgat catatagcgg cccttaggga tcccgctagc cttgctatgg acatgattga
      541 gaatttggac tttgtggtcc aaaagctaaa agaatcctac aaggactatt accatgttta
      601 tgatatattc tataatatgc taagggatgc gggcatgccc ataacctctt ggacacccct
      661 attttatgaa ggacgatact acataccatc caatgacttt tcctgcatga tcagcaaaaa
      721 aatgtttgat gaggtctttg tgccgggaat aattgaggag tgtaattttt acgacaggtc
      781 catctaccat ctggacggcc ccggtgccct ccggcactta gattcaattt tggctattga
      841 aaatctaaat gccgtgcagt ggatttgcgg cgcaggtaac gagggctacg caaaatggat
      901 cgatgtatat caaagaatcc aggatggggg taagggcctg cagctaatga taactctgga
      961 tgaattgccc ctggtctttg aaaccctgag acccgaaggg gtctggtttt cgaatatcta
     1021 tggtatagat gatatagtca cagccgaaag ggtagtaaag cgtatcgaag cgtggaaata
     1081 agaacacagc ggaaccgttc ccttgtgttg ggggaaccgt cgccctgtgc tgtgtgttgt
     1141 ttgagagggt tgatagaatg aaaattttac atattgcaga tttacatatt ggaaagcgcg
     1201 tcaacggatt cagcatgata gaggaccaag agcatatatt ggatgagatt ttaaggataa
     1261 cacgggagga ggaacccgga gctgttctga tggccggaga tatctacgat aagagccaac
     1321 cctccgccga ggctgtggaa ttgttggatg agtttctgac gagactgact tccctggggc
     1381 aacccgtgtt tataatcagc ggcaaccatg attcccccga gcggctgagc tttgggagca
     1441 gaataatgca aagcaacgga ctccatattg cgggggtatt tgacgggtcc cttgagaaga
     1501 tgaccttaga ggacgagcat ggcatcatca acatatatat gctgcccttt ataaagcctg
     1561 ccatggtcag gccgtttttt gaagaaccca tagaatccta cgaagaggca gtgaagacgg
     1621 tgatttcggc gtcttcagtc aatgcggacg agagaaacat ccttgtagcc catcagtttg
     1681 taataagcgg aatgaagcag ccccagcagt cggactcaga ggccatatcc gttgggggat
     1741 tggaccatgt ggacgcatcc gtcttcgatg attttgacta tgtggccttg gggcacctcc
     1801 acggtcccca gcccatggga caggattgga ttcgatacgc cggttcaccc ctaaaatact
     1861 ccttttcgga ggctcgccat accaaatcta tgaccatggt ggagctgggc ggtaaggggg
     1921 agatttcaat agagcaaatc cccctcgctc ccctaaggga cctccgacaa atcaagggac
     1981 ctatcaagga gcttatcagt gtcggatcag aggatgcaga gggtgccatg gattatatcc
     2041 acgccatttt aaccgacgag gaagagatct atgatgccat cggacaatta aggcaggtct
     2101 atcccaatct aatggcctta gaccttgaaa atagccgtac ccagcaggta tatgattccg
     2161 aagtccttgc tgccagcgat tccgcagaaa aggacccctt ggatctgttt tctgaattct
     2221 accagataca gaacaacaac gatctaactc gggaggaaat acgggttatg aaggaaattt
     2281 ttgagaaggc ggggggtgtc gggatatgaa accaataaaa ttggttatga gcgccttttg
     2341 ttcctatccg gatagagttg aattggatat gaccgcattc aaaggcaggg gtctattcct
     2401 cattacaggc gatacaggat ctgggaagac caccatcttt gatgccatgg cttttgccct
     2461 attcggggag gccagcgggt ctactcgggg ggttgaaacc ctgcgcagcg actttgcaaa
     2521 gcccgacacc gaaacctatg tggaatttac ctttttgcat agggataagc aatacactgt
     2581 aacccgaaac ccccgctacg aaaggccaaa gaggagcggg caaggcttaa ccaccgaaaa
     2641 ctccaacgct accttggagc ttccaagcgg ggatattgtt accggcctca ataatgttac
     2701 ggcaaaaatt gaaagcatac tgagcatcaa ttgccgtcag tttaagcaaa ttgccatgat
     2761 cgcccagggc gaatttttgc agctattgct atccagtacc aaggatcggg gtgaaatttt
     2821 tagacgggta tttaataccg acctctacca gacagctcag agactattaa aggaggggga
     2881 gcgggaagcc aagaagcgct gcgagagggt tgagctgagc atcctccaat atatttccga
     2941 gatttccgct tctgaaagcg aaagaggccg agaattatcc cttaagatgg gcacagccaa
     3001 tatacacaca gcaggggata tttacattgg gctacagggc cttattttag aagacacgat
     3061 caaaagaaac gatctaaaga agcagtcgga tgagctggag caaaggcttg cagggcagat
     3121 cgccaggata acggagggcc agtacataaa tcaatccttt gaggatttaa aggctgtagt
     3181 gaagagagaa aaagttttaa ccgagagcct aaaaaggcat aatagagaaa agaaagattt
     3241 ggagatggcc gaaaaagccc tatacaccat ctccccttta gagatcgcat ataggcggga
     3301 aaggaaggtc agagaggggc ttattgagaa tatcacaaag ctgacccaga ttatcgaagg
     3361 tcaagaaaag actcttgggc tagctagtga aagttataat atagaaagaa aaaaagagcc
     3421 ggatcgggaa agcctcacct cggccataga cagccttaaa aagatggtgc cggcctatga
     3481 aaatgctcag acgctggaaa aggatctaag ggctctatcg gctaggcaca agggtctaac
     3541 ggaagaattg gaaaagctga aactgcaaaa ggatgggctt atggataata aaaaggcgtt
     3601 gggtacggag ctggaacagc tgggggatat agagataagg acatccaatt gtcagcaaga
     3661 cctcagacag ctggaaactg ttttagccca attagtcgag cttcaaaatt atttggccca
     3721 atataacaaa ctagaagttg aaagccacaa actaaaaaag cagtttcaaa aggcacagga
     3781 taaatataca attgttaata aggactacct ggaaaaggag acagccttct tccgggagca
     3841 ggcggggatt atggcggctg gccttaagga aggggagcct tgtcctgtat gcggctctag
     3901 ggcacatccc aataaagcca taccggcgtc agatgccccc agcgaagcgg aacttaaaag
     3961 gctcaaggag aaaacagacc tagcccgagg agatatggaa gaggccagta acaaatcggc
     4021 aacgaagctg acggagataa aactggccaa ggaacagcta ttaaaggctt caaaggtgca
     4081 ttttttaaaa ctggacgaag aaccctggga aggggtccta gaatccctga tagaatcgga
     4141 actggagcaa aataacgaga agaagaaagg gttggatatt aaatacctca agctaaagga
     4201 tgaggttacc cgtaaatcag aggcagcaaa acagcttaag cttttggagg aatccctcca
     4261 aaactgtgaa gaacagatga ataaggtgga agaggaaaga aataatgtca atacagactt
     4321 ggcctctaaa gagggggagc ttaagaattt aaaatcttca ctggaatatg aaaatcgtca
     4381 gcgggcggag atgaccataa aggaatggga ggtccggctg gccgatctga aggccgcctt
     4441 tgataaagct gaacaggcct accacaaggc taaaaacgag ttgaacaaca gccaaaccct
     4501 attaaaagat catagagaaa ggctaaacga gactgaggag accaagaaac aagcccagat
     4561 agcgtatact gaaggtataa tcaggtccgg ttttaaggat gaatcggact atcaggaagc
     4621 actgaggacg gagccagaaa ttagggagct tagaggatcc atcgacgagt atataagaga
     4681 ggttcggtcc gtagaccagg atataagaag gcttcgggag gaaacaaagg ataaagagga
     4741 acaggatcta gagggtctaa aggccataaa gaagaacttg gaggaggcaa aaagtaaggt
     4801 gggagaatct gcccagggtc tggtgacccg gcttgaagcc aataaatcca ttctaaaggc
     4861 tctggagaag gctatcccca aggcaatcag ctatcagaag gaatatcttc tattgggtaa
     4921 cctttcaagg acagccaatg gggagctttc cgggaaacaa aagctggctt ttgagcaata
     4981 tgtacaagcc tcctacttta accgaatact catcgaatcc aataaaaggc tgcggctcat
     5041 gaccaacaat agatttgagc ttctcagaaa agaggatgcg acagatctta ggtctagggg
     5101 acttgaaatt gatgtgctgg atcattacac cggtagggtg cgctcggtaa aatccctatc
     5161 cggcggggag tcctttaagg catctttatc cttggccctg gggctgtcgg atgtgatcca
     5221 aagtcatgcc gggggcgtgg agatcgatac cctgtttatt gatgaaggct tcggggcttt
     5281 ggacgccgag tccttagagc aggcaataca aacccttgca ggattggcgg aaggaaatcg
     5341 tctggtggga atcatctccc atgtaagcga gttaaaggag aggatcgacc gccagatctt
     5401 gattaaaaag accccaaagg gcagtgccct tagtattacc ggtgcctaga acataagggg
     5461 aacacagggg gacggttccg ctgtgttgta cgattaagtc gcacaaacac gcagaaaccg
     5521 tcccctgtgt tcgttccatg ttgttaagtt ttatagtttt ttaccactaa ttattttttc
     5581 accggatcgt acccaacaac gctccatatt ccatcatcat tcgatttaac cagtcttttt
     5641 aggtatacct ttgctatggg tgttttatca ccgctgatct ctatgatagt cacctcgtta
     5701 gtctcatagg caattttcat atcctcggtg tccacgggaa aatcaccttg gataccgtct
     5761 ggtgatattt gaatccctac aaaggtttga gctacggcaa aggggcttaa ctgccatggc
     5821 gaatgcccct cgtccacctg tttctgatcc ctttccgcta tctccatatc tatttcaacg
     5881 gatacctgag gtttttgtgg gaagctatta tccgtatcta ctacttcgtg gacttcatcc
     5941 attttagagg attccacatt ttcaacggcg ctatcagggt tttccttcat ttgattttcc
     6001 cggattaaag ctacgtcatc cttatctttc gttgataggg gaggtttacc caaaccttta
     6061 gccctggtta aaccattggt caaaccgatt ccgccaatta aaatgattgt cgcagctgcg
     6121 attattgtcc atctaaaggg tatagtatta aactttctga tcatgattat tctcctctta
     6181 agctctgatt tattcgttgc caacccgaca gttccgggat tccaataggg ttttgataaa
     6241 attgctaata aactaataat agtatttcca taatccttac gattttccgg ttcaagatat
     6301 gccaatacat gggcatcgca agcaatttca cagtcacttc gcatctttcg gaaggcatac
     6361 cacactactg gattaaacca atgcaatgcc tgtatcgtta ccataatcca atttattaat
     6421 atatcccctc gtcttaagtg caccatctcg tgaagaaata catattcaag ctcttcagga
     6481 gacagtccat tcgccagttg atgtgatatt aggatcttcg gcttaaaata tccgaagagg
     6541 gaaggtgctg ttgcctgagg actgtataca atccgaggta gcctttttgt ctttgtcttt
     6601 tttcttaagc gttccagaat ctccgccatg ttgtcctcta cgcattcagg catattatta
     6661 atcttgtaaa gcaggataga gtatattcca agattataga ggattatcgc aagtacacct
     6721 gtagcccaaa taccttttac aacgtttatc caagattgtg ctttgaaata gtcgtttcga
     6781 ttatggatag tatcggattt atcccccggg ccttcatgta atatttgccc gctatcatct
     6841 tctctttggg tgggttgctc tacatcggca ttgcttgata aattaaggtt atggcctaca
     6901 tcaaactgag gtcttgtata tctctctata ttttcaaaaa cctgtggaac caaattaaat
     6961 atgctaaagg atgtttcagg agcgtagggg ataattagtc taaccataag taagaaccag
     7021 atataataat gaaatgcggc attggattta tcccttagca aggcttttat aagcattata
     7081 atcccagcca ctacacttcc cataagggac atagtaatta ctgattgaaa taatttatgt
     7141 aggctcaaac cttgtcacct ccttaaaatt cttggtctaa tcgagtatgc gtcttagttc
     7201 ttcaatctct tcacgtgaca gctcttcact ttcaacgaag ctgactaata gccttttcat
     7261 agaaccatca aagatttttt ccaaaaagaa cttggtttct tccctcatac attcttcttg
     7321 agatacccgt ggatagtatg catatggcgt gacaccctta tcaacattca gagccccttt
     7381 gtcaacaagg cgaccaagta aggtatgaat ggtcttgggg ttccaagtgg tatagttttg
     7441 caattcgttg ataacttgac tggcatttaa gggggattgc ttccataaaa ccttcataac
     7501 ttgccattct gcatcagaaa ttttgggtaa ctccctcatc ctcaccacct ccttcctaca
     7561 actgtagtct acgaacacat attacacctg tagtcccgaa atgtcaatcc ctattttcta
     7621 ataattccat ataaaattca taaaagcatc atagctaaac tacctataca atagatattg
     7681 aaacgatttt ttaaacacca tatgctttac gataaacact ctacattaag attcatacaa
     7741 cgtaaaatat tttcctataa aatgtggatt ttattataaa agatagttcg tgtagcagaa
     7801 tcatatttac acatgtatcc gcaacggttg actatttatt tttaaaggga ggtttcctac
     7861 aaatgatcaa agttagtgtg ctgggcgcca ccggatatgc agggatagag cttataaggc
     7921 t
//
LOCUS       DASRNX010000050         6463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_61910,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000050 DASRNX010000000
VERSION     DASRNX010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61910"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VFD89_01905"
     CDS             complement(<1..1052)
                     /locus_tag="VFD89_01905"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HZJ56974.1"
                     /translation="MRKIGIKHAKPGMKLARSIINATGHVLIGRGVVLTHRYIDRLQD
                     KGVYHLYIEDDISEGIEIEDIVSDRTRNYAQVVIKDVMDDIVWQNKLNRVPEITKIVN
                     NIVDELLDNDDILVNIKDIRMIDDYTFGHCTNVCILSIITGIELGYNELRLRDLGVGA
                     LLHDIGKMRIPKHILQKPGQLTSEEYEQIKQHTVFGFEILRNCDDIRKPAANAALGHH
                     ERFDGSGYPRGLTGKDTHEFAKVIAIADVYDAMTSDRVYRTGMEPNEAARYLTSMGDT
                     LFDNELVNTFMKSIAIYPVGSSVILNTGEKGLVSKTHSDHPNKPTVRIIYNPQGDKYN
                     SFKEVNLAMNPELTIMR"
     gene            complement(1229..1513)
                     /locus_tag="VFD89_01910"
     CDS             complement(1229..1513)
                     /locus_tag="VFD89_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZJ56975.1"
                     /translation="MKSTKVRIMHDAGLNARPAALFVQVSSKYNSRIWVKVGTKKVNA
                     KSIMGIMSLSVVSDDEITIIAQGEDAQEAINSLTELVDSNFSHMPVIEKA"
     gene            complement(1510..2475)
                     /gene="whiA"
                     /locus_tag="VFD89_01915"
     CDS             complement(1510..2475)
                     /gene="whiA"
                     /locus_tag="VFD89_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HZJ56976.1"
                     /translation="MSFSSRVKNELCHIGDQPPCCSLAELAALIQICGSIHVSGRQGV
                     SLRVNTENPGVARRIFLTLKRLYNIQAEIAVRKKRRLRKNNSYYLVLSKNQQAMRVLM
                     DTGIVRKRDGGDLWINRTIDQTIVRKRCCKRAYIRGAFLGGGSVSDPEKIYHLEIVTH
                     NEVYADSLASLINEFGLSARTIGRKNNFVVYLKDGGHIVNLLNIIGAHAALLDLENIR
                     IYKDMRNNINRIVNCETANLGKTINAAVRQIENIQYLRDHYGLSKLTPPLREIAELRS
                     NNPDASLKELGQMLDPPIGKSGVNHRLRKLDQMADNIKNKRGRTS"
     gene            complement(2524..3381)
                     /gene="rapZ"
                     /locus_tag="VFD89_01920"
     CDS             complement(2524..3381)
                     /gene="rapZ"
                     /locus_tag="VFD89_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003360288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZJ56977.1"
                     /translation="MKFLIVTGLSGAGKTMAIRYLEDMGFFCVDNLPPKLILRFVELC
                     QESEGQIDKVAIVVDIRGRGFFDDLLGCLSDMKEEGHRYEILFLEASDNVLIKRYKES
                     RRAHPLVREGRIISGISKEREVLERIKGISDHIIDTSNLYPKQLKEALVELFHDQHDN
                     DGLVISVISFGFKHGILLDADLVFDVRFLPNPFYVDELKMHTGQKPEVRDYVFKFAET
                     KEFMDRTIDMLEFLIPYYVKEGKFQLVVGIGCTGGMHRSVAIGDALYEDLQEKGYRAS
                     VEHRDISDA"
     gene            complement(3407..4147)
                     /locus_tag="VFD89_01925"
     CDS             complement(3407..4147)
                     /locus_tag="VFD89_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZJ56978.1"
                     /translation="MMYNITADYHTHTRYSHGKGTIEDNVMAARDKGLKRIAITDHGF
                     GHIGFGLKYRDIAEMREKIQELNEKYPDIDILLGIEANLTGLDGTIDIPNAYIDKFDI
                     ILMGFHKAVMPLSFHDAKELFIKNALSMAFPIDKERLRQQNTRAIVNAIRRYPIKIIT
                     HPGAKIDIDTKMLAKHAVERGVFLEINASHGFMTVEYVKIAMKEGASFVINSDAHHPT
                     RVGDFRKGIEIASSAGLGPEQIMNAEVD"
     gene            complement(4144..5064)
                     /gene="murB"
                     /locus_tag="VFD89_01930"
     CDS             complement(4144..5064)
                     /gene="murB"
                     /locus_tag="VFD89_01930"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZJ56979.1"
                     /translation="MDMSMEIAYKRLNEYIPHQRLLVNEPMKNHTSFKIGGAADILVI
                     PSENREVVHVLRACNDLSVPVFVMGNGSNLLVRDGGIRGVVIKLSDNFSDINIEGNSV
                     KAQSGVLLSTLSESATENSLRGLEFACGIPGTLGGAVAMNAGAYGGQMEDIVVSVATV
                     DRQGNYHAFQPQDMNFGYRTSSIQTNGHIILDVTMELEHGDLQESLELVDDLTRRRAE
                     KQPLSYPSAGSTFKRPVGYYAGKLIQDSGLKGLRVGDAQISDLHAGFIINLGNATAKN
                     VIDLICKVQKIILDKYGVEMIPEVRIVGEE"
     gene            complement(5506..>6463)
                     /locus_tag="VFD89_01935"
     CDS             complement(5506..>6463)
                     /locus_tag="VFD89_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZJ56980.1"
                     /translation="YSYKAIKLPKYLPKDGFTTLIIGFVRLKGNQLTIPYSNLFKKDH
                     KRITINIPPVLAGKKIKEIRIIPKAKARFFEIQYIYEAEESQRELDKNRALAIDFGIN
                     NLATCVTSTGQSFIIDGRRLKSINQWYNKENSRLQGIKDLQGYEFQTNRQKALVRNRN
                     NKVNDYMSKAARKIINYCLEHNIGNLICGFSETFQRNSNIGKANNQTFVNIPFGRLRE
                     KLEYLSELYGITFLKQEESYTSKASFFDKDEIPVYNDDNPHEYKFSGKRIKRGLYRTK
                     SGYTFNADVNGALNILSKSSVVSLTGLYSRGELDTPVRIKIA"
BASE COUNT         1661 a         1580 c         1167 g         2055 t
ORIGIN      
        1 cgcattatgg tcaattccgg attcattgct aggttgactt ctttaaatga attgtactta
       61 tccccttggg gattatatat tatcctaaca gtcggcttat ttggatgatc gctgtgggtt
      121 ttagatacca atcctttttc gcctgtattc aatataacgc tactcccgac ggggtatatg
      181 gcaatgcttt tcataaaggt gtttaccagt tcattatcaa acaatgtatc gcccatactc
      241 gtcaaatatc ttgccgcctc gttaggctcc atacccgtac gatataccct atcggaagtc
      301 atggcatcat aaacatcagc aattgctatc accttcgcaa attcatgggt gtccttccct
      361 gtgagacccc tagggtaacc gctcccatcg aacctttcat gatgccccaa cgcggcgttg
      421 gctgcaggtt ttctgatatc gtcacaattt ctcaatattt caaacccaaa gaccgtatgc
      481 tgttttatct gctcgtattc ttcactcgtt aactgtccag gcttttgtaa tatatgctta
      541 ggtatcctca tctttccaat atcatggagc aaggctccta cccctaaatc cctcaggcgc
      601 agctcgttat atccaagctc aattcccgtt atgatagata ggatacacac gtttgtacaa
      661 tgaccaaatg tataatcgtc aatcatccta atatccttaa tatttaccag aatatcgtcg
      721 ttatccagaa gctcatctac gatattattt acgatcttgg ttatctcagg cacccgattc
      781 aatttgttct gccatacgat atcatccata acgtctttaa tgaccacctg agcataattt
      841 ctggttctgt cggatacaat atcttcgatt tctattcctt ctgatatatc gtcctcaata
      901 tacaggtgat aaactccttt atcttgaagc ctgtctatat accggtgtgt gaggactacg
      961 ccccgtccaa ttaatacgtg tccagttgca ttgattatcg atcgagccag cttcataccc
     1021 ggcttagcat gtttaatccc aatcttccgc attgctccct ccaactgcgt gaagtacagc
     1081 aggccaagat ctccactgta tttaactgtt caaaatcaaa aatagcggcc ataaatactt
     1141 cagatacttt acatgaaata ttatacaata ttcaacaaaa ttcagcaata cggcgtaggg
     1201 acgctttata taggactaaa gaactgtttt acgctttttc aataacaggc atatggctaa
     1261 aattggaatc caccagttcg gttaaagagt taattgcttc ctgggcatct tccccttggg
     1321 caattatcgt aatttcatcg tcggatacaa cgctcagaga cattatgccc ataatgctct
     1381 tagcgtttac tttctttgta ccgaccttta cccaaatcct ggagttgtac ttgctagaca
     1441 cctgaacaaa cagtgccgcc ggtcgggcat tgagccctgc atcatgcatt atcctcactt
     1501 ttgtactttt caactggtcc tccccctttt gttctttatg ttatccgcca tttgatccaa
     1561 ttttctcaag cgatgattga cgccggattt accgatgggg gggtccaaca tttgacctaa
     1621 ttcctttagg ctggcatctg gattattaga cctcagttca gctatctccc taaggggcgg
     1681 agtcagcttg gacaggccat aatgatctct tagatactga atattctcta tctgtcttac
     1741 tgccgcattt atcgtcttgc ccaaattggc agtctcacaa ttgactatcc tgtttatatt
     1801 attgcgcata tccttatata tcctgatgtt ttccagatcc aatagcgccg catgggcacc
     1861 gattatattt aataagttga caatatgccc cccatctttc aaatacacca caaagttatt
     1921 ctttctccct atggtccttg cactgagacc aaattcgttt atgaggcttg caaggctatc
     1981 agcatatact tcattatgag ttacgatctc caaatgataa attttttccg gatcgctgac
     2041 cgaaccgccg ccaagaaaag cccctcttat atatgccctc ttgcagcacc tttttctgac
     2101 aatggtttga tctatagtac gattaatcca taggtcccca ccgtcccgct tcctgactat
     2161 tcccgtatcc atcaagaccc tcatggcctg ctggttcttt gacagcacca aataatagct
     2221 gttgtttttc ctcagcctcc gtttttttct tactgcgatc tcggcttgaa tattgtaaag
     2281 tcttttaagg gttaaaaaga ttcgcctggc aacgcccgga ttttctgtat ttacacgcag
     2341 ggataccccc tgtcttccac taacgtggat actaccgcaa atctgtatta gggcggctaa
     2401 ttcggcgaga ctgcaacaag ggggctgatc ccctatatgg caaagctcgt tcttgactct
     2461 ggatgaaaag gacatctatt tcactcctta aaaattgggc gatgtaatac cttgtgatag
     2521 ccattaagca tctgaaatat ccctatgctc aacagaagcc cgataaccct tctcctgcag
     2581 gtcctcatag agtgcgtccc ctatggctac ggatctatgc atacccccgg tgcaaccaat
     2641 ccctacaacc agttgaaatt tgccttcttt tacatagtag gggatcaaga actccaacat
     2701 atctatcgtt ctatccatga attctttggt ttccgcaaac ttaaagacat aatcccttac
     2761 ctcgggcttt tgacctgtgt gcatctttag ttcgtctacg tagaagggat tgggcaggaa
     2821 ccgtacatca aaaaccaagt cggcatccag caatattccg tgtttgaacc caaaggatat
     2881 gacggatatt acgaggccgt cattgtcatg ctgatcatgg aagagttcga ccaaggcttc
     2941 cttcagttgt tttgggtaaa ggttgctggt atctattata tgatctgaaa tccctttaat
     3001 cctctctagg acctccctct ctttgcttat accggagata attctcccct ccctcaccag
     3061 gggatgggcc ctcctgctct ccttatatct ctttatcaga acattatctg aggcctcgag
     3121 aaaaagtatc tcataacgat gtccttcttc cttcatatcc gaaagacagc ctaaaaggtc
     3181 gtcaaaaaat ccccttcccc gaatatccac tacgatggct accttatcta tctgtccctc
     3241 agattcctga cataattcta caaatcttag aatcagctta ggcggtaggt tatctacaca
     3301 gaaaaagccc atatcctcta aatatcgaat agccatggtt tttcctgcac ctgataagcc
     3361 ggttacaatc aagaatttca tatccatacc ctcttcctca gtaaagttaa tcaacctctg
     3421 cgttcattat ctgttccggt cccaggcctg ctgatgaggc aatttcaata ccctttcgaa
     3481 aatccccaac ccttgtagga tgatgggcat cgctgttaat aacgaaactg gccccttcct
     3541 tcatagctat tttaacatat tccacagtca taaatccatg gctggcgttg atctccaaga
     3601 acacgcccct ttctaccgca tgcttggcca acattttagt atcgatatct atctttgccc
     3661 cgggatgggt gatgatttta atcggatatc tgcgtatggc gtttacaata gcccgggtgt
     3721 tctgttgtct caatcgttcc ttgtcgatag gaaaagccat ggataaggca ttctttataa
     3781 ataattcttt tgcatcgtga aatgagagag gcatcacggc cttgtgaaac cccattaaaa
     3841 ttatatcaaa tttgtctata tatgcattcg gtatgtctat ggtgccatct aaacccgtta
     3901 agttagcttc aattcccagc aggatatcta tatcgggata cttttcattt agttcttgta
     3961 tcttttccct catctcggct atatccctat actttaaccc aaaacctatg tgcccaaacc
     4021 catggtcggt aatggctatt cgctttaatc ctttatcccg agcagccatt acattatcct
     4081 ctatggtgcc tttaccatgg ctatatcgcg tgtgggtatg ataatccgca gtgatattat
     4141 acatcattcc tccccgacta tccttacctc ggggatcatt tcaaccccgt atttgtccaa
     4201 aataatcttc tgtaccttgc agataagatc aatgacattt tttgcggtgg cgtttcccag
     4261 gtttataata aaaccggcat gcaggtccga gatttgggca tcccccaccc ggagtccctt
     4321 taatccggaa tcttgaatta gtttgcccgc ataatagcct accgggcgct taaaggtgct
     4381 ccctgcgctg ggatatgaaa ggggctgttt ttctgccctt cttctggtta aatcatctac
     4441 aagctccaaa gattcctgta ggtccccatg ctccaactcc atagtcacat ccaaaattat
     4501 atggccattg gtctgtatgg agctggtacg atacccaaaa ttcatatcct gcggctgaaa
     4561 ggcatgataa ttcccctgcc tatccaccgt ggctaccgaa actactatat cctccatctg
     4621 accgccgtag gcccctgcat tcatggctac agccccgccc aaagtcccgg gtatgccgca
     4681 tgcaaattcc aggcctctca ggctgttttc tgtagcactc tcagacaggg ttgataacag
     4741 caccccggat tgtgctttaa ccgagttacc ttcgatgttg atatcgctga agttgtccga
     4801 cagctttatt accactcccc ttattccccc gtccctaacc aaaaggttag agccgttgcc
     4861 catcacaaat accgggacag aaagatcatt gcacgccctc agtacatgga cgacctcccg
     4921 gttctccgat gggataacca gaatatccgc ggcgccccct atcttaaagg atgtatggtt
     4981 tttcatcggc tcatttacca gcaagcgctg gtgtggtata tattcgttca atctcttata
     5041 ggctatttcc attgacatat ctattccact ccctattata ggaattaatc ttcgctctcg
     5101 cttcctcctt tagtttacat taaatcctag cttcattcaa tagctttccc aacctatgga
     5161 atctaaagga cagcctttat tattttactc atctctttaa tttctatata gtatagtctt
     5221 aaaaccaaaa gtgttcgata aagcccgtat aatgatgtaa aaagaaaaat gagttgagcc
     5281 taagccctac ctcggttaag atataggtac taccaaacat caagctaagg tgtagtgata
     5341 attatatgga taaaatgcga aagatcatag ttgatttcta agtagaaaca tcaatgattc
     5401 gtccatggga gatctaccta aaaggggaat aatgaattct gaacctccca tgactaaagt
     5461 cacggggttc tagagttcaa atgaactcat ttacgcctct gtagcctaag ctattttaat
     5521 gcgtacaggc gtgtccagtt cgcctctact gtatagccct gttaaggaca caacgctact
     5581 tttacttaaa atgtttaatg ctccgtttac atcagcattg aaggtatatc ccgatttagt
     5641 cctgtatagg ccgcgcttga tacgtttccc ggagaattta tattcatggg gattatcatc
     5701 attgtatact ggtatctcgt ccttatcaaa gaagctggct ttagaggtat agctttcttc
     5761 ttgtttcagg aaggtaattc cgtacaactc tgatagatat tcaagctttt cccgcagcct
     5821 gccaaaggga atatttacaa aggtttggtt attggcctta cctatattac tgtttctttg
     5881 gaaggtttca ctaaagccgc agattaggtt gcctatatta tgctctaaac aatagttaat
     5941 gatttttctg gctgcctttg acatataatc attaacttta ttgttcctgt ttcgaacaag
     6001 cgccttttgg cggttagtct gaaattcata accttgtaaa tccttaatac cctgtaatct
     6061 ggagttttct ttgttgtacc attggtttat ggattttagc cgtctgccgt ctataatgaa
     6121 actctgacca gtagaagtaa cacaggttgc cagattattt atcccaaagt ctatcgccag
     6181 tgctctattt ttgtctaatt ccctttggga ttcttctgct tcgtaaatat attgaatctc
     6241 aaagaacctg gcctttgctt tcgggattat ccgtatctct ttaatctttt ttcccgccaa
     6301 tacaggggga atgttaatag ttattctttt gtggtccttc ttaaacaggt tagagtatgg
     6361 tattgtcagt tgattgcctt ttagtcttac aaaaccaatg atcagcgtag taaagccatc
     6421 tttcggaaga tatttgggaa gctttattgc cttgtacgag tag
//
LOCUS       DASRNX010000051         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_381415,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000051 DASRNX010000000
VERSION     DASRNX010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381415"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1817
                     /locus_tag="VFD89_01940"
     CDS             231..1817
                     /locus_tag="VFD89_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HZJ56981.1"
                     /translation="MEAGARIRKNIWGKIFLIFILTVLIVAIGGTVTYGYQILNTDTF
                     YEGVEVDGIPLGAMTRDEALNVIRAHNQPSLDKMQITLTHGDKRWEYDYRDINAEINI
                     EEIVDEAFLVGRQGTIAERLKEIYHVNGEPKRYKTTLTYDVSQLQGKVKAISNEINEE
                     PVDATIAFNPDNKEKFTFTSDQAGIGMLVDKTMEALTARVDAGDFSPFSIATEDLLPQ
                     YTLEEVKTWTSRIGYYSTPLNNNSNRNHNIKLSSKAYYNVRLDPGETYSLNETTGPRG
                     AAQGYKSAPVIKDGNRFEDEPGGGNCQTSTTLYGAALRADMEIVERAPHSIKSTYTPV
                     GTDATVSYPYADFKFKNNKDTPIFITRYMSGGKLHVEIYGKQSEEFDEIKVVSWQTGT
                     TDVPEYKIVKDATMYEGEEKIEYQSRAGIKAVSYKVYYKNGKEIKRVKAANSSYRRIQ
                     GLKRVGTMKKAQEEPADKPKDKPEDKPKDKPKDKKTEDEKPKDPEPKEPNTGEPKPKD
                     PKPEKPKKEDPKPEPSDGEE"
BASE COUNT          763 a          486 c          532 g          561 t
ORIGIN      
        1 cagccataaa tacaagatag tattttacgc ccgaaatagg gccttttttt tgcccgaatt
       61 ccggccaatt gttacgatag agttaactgg aattaaataa atattaactt agttacattt
      121 ttacttcacg gcatttaatt cttcagattt tgctggaaat tctatttact tttttgggcg
      181 cataacctat gataaacagg taaatgaaag tgtcgatgga gggaatacat atggaggctg
      241 gagcaagaat tagaaagaat atatggggta agatttttct catatttatt ctgacggttc
      301 tcattgtagc tattgggggt accgttacat acggctacca aatcttaaac accgatacct
      361 tttatgaagg tgtcgaagtg gatggcatcc ctttaggggc aatgaccagg gacgaggcct
      421 taaatgtgat aagggcccat aaccaaccaa gcttagataa gatgcagata accctcaccc
      481 atggggataa gagatgggag tatgactata gggatatcaa cgccgagata aacatagaag
      541 agattgtaga cgaagcgttt ttggttggca ggcaaggaac cattgcagaa aggctcaagg
      601 agatctatca cgtcaacggt gaaccaaaaa ggtataagac tacccttacc tatgatgtgt
      661 cacaactcca gggtaaggta aaggctattt ccaatgaaat aaacgaggaa cccgtggatg
      721 caaccattgc atttaatccc gacaataagg aaaagttcac ctttacttcg gatcaagcgg
      781 gcataggtat gctggtggat aagaccatgg aagccctaac agcacgggta gatgcgggag
      841 atttttcgcc ctttagcatt gcaacggagg atttactacc ccaatatacg ttggaggagg
      901 ttaaaacctg gacctccaga attgggtatt attccactcc ccttaacaat aattcaaacc
      961 gaaaccacaa tataaagctt tcatccaaag cctactataa tgtacggttg gatcccggtg
     1021 agacctattc cctaaatgag accaccggtc ccagaggggc agcccaagga tacaaatcgg
     1081 caccggtaat taaggatggc aacaggttcg aagatgaacc cgggggcggg aactgccaaa
     1141 cctccacaac cctatacggc gcggctctaa gggccgatat ggagatcgtg gagagggctc
     1201 cccattccat aaagtctacc tatacgcctg taggaaccga tgccaccgtg agctatccgt
     1261 atgcggactt taagtttaag aataacaagg ataccccgat ctttataacc agatatatgt
     1321 ccggcgggaa actccatgtc gaaatatatg ggaaacagtc cgaggagttc gatgaaatta
     1381 aggttgtcag ctggcaaacc ggcacaaccg atgtacccga atataagatc gtcaaggatg
     1441 ccaccatgta cgagggggag gaaaagatcg aataccaatc ccgtgccgga ataaaggccg
     1501 tatcatacaa ggtctattat aagaacggca aggaaataaa gagggttaag gcggcgaatt
     1561 ccagctacag gaggattcag gggcttaaaa gggttggtac tatgaaaaag gcccaggaag
     1621 agcctgctga taagcccaag gataagccgg aagataagcc taaggataaa cccaaggata
     1681 aaaaaaccga agacgaaaag ccgaaagatc cagagccgaa agaacccaat acaggggaac
     1741 caaaaccaaa ggatccaaaa ccggaaaagc caaagaaaga ggatccaaaa ccggagcctt
     1801 ctgacggtga agaataatat gcaaaactaa ttccatatag aaatatgtcg ttttggccca
     1861 taacaaagaa aacagttttt gaatgtttta tatgggtgga tggtttattc tggcattttc
     1921 caaactacat cgggaagctg ctttataggt cccatctttg ggggattatt gacggcactt
     1981 ccctaagggg tctttctttc gggccgttgg aatctgcctc cacaaatcca tcctccccca
     2041 gtaacagcta caatgggcta ggcttctaat ttccaatcac gggctttcaa aaatcaaata
     2101 aatgggatca cccatccata aatattggta ccaacatatg cttaattggc acaatgtgct
     2161 gaccctgacc ccgcaacaaa gacggggggg ttaattttgt cctttaactt tgcatgcatc
     2221 tattctataa tgttatccgt agggtgaagg aaactaagtc tacagataaa caactccgac
     2281 tataaaattt ataatataca gataaatcta gttttcctat tgtttatcta tacatggcat
     2341 ac
//
LOCUS       DASRNX010000052        40932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_646882,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000052 DASRNX010000000
VERSION     DASRNX010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 40932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40932
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646882"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..875)
                     /locus_tag="VFD89_01945"
     CDS             complement(102..875)
                     /locus_tag="VFD89_01945"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZJ56982.1"
                     /translation="MILVIDIGNTNVVLGVYDGQDLVTSWRLTTDRDKTGDELGILVL
                     DLFRYSRISEDDISGIIISSVVPTLMDALELMCKKYFDIEPLVIGPGIKTGMNIRIDD
                     PKEVGADRIVNAIAAYETYRSPLVIIDFGTATTFCAVSAQGEYLGGCISTGIKISTEA
                     LFQYAAKLPRIELIKPTNIIGKNTVESMQAGIVYGYIGQVDYIVRRMKRELNAPNVKV
                     IATGGLATLIARDSETVDEIDENLTLRGLKIIYERNQSR"
     gene            complement(949..1551)
                     /locus_tag="VFD89_01950"
     CDS             complement(949..1551)
                     /locus_tag="VFD89_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HZJ56983.1"
                     /translation="MKITTRKLAVIGMLGGISIMLEFGGVGIIQLPFLPVQVTIMHIP
                     TIIGAIVEGPVVGLFIGLIFGGFSMYRAFTQPSVLSVAFMNPLVSILPRLAIALTSYY
                     SFKGIKNLLRGKGQRGIALSTMVASAVGTLTNTIGVLGIAYLLYKDVFAKMLNVEQSM
                     VGKAVIYGIAIPNGVPEAIAAVLIITPIVQALRKFYKGDF"
     gene            complement(1702..2988)
                     /locus_tag="VFD89_01955"
     CDS             complement(1702..2988)
                     /locus_tag="VFD89_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZJ56984.1"
                     /translation="MVEGKLRGQLRETGNILKGFKGNARVCIMTEPLWGIPYNLFITY
                     ASVYMAALGCTEKEIGLIASVGLMFQMFFSLVSGHITDKLGRKRTSLIFDLISWSVPT
                     FIWAIAQNFYYFLAAAIINSAVRVVHTSWTCLFVEDTSSEDRVHVFTWMQVAGILSGF
                     VAPVAGFFVSKYRLVPAVRGLYFFAFISMTFMFILRHFLAHETAQGLIRMKESENGGF
                     FDSLKDYKRAASQLLAAPYTMIAFLLSIFTNIHTVIRTNFFAILLTKKLEFSQGSIAL
                     FPMIQSGIMLLVLLFVMPILGKTNIKKPLLGSFISLIISNLLLVVSPNRSYTVLIIST
                     IFMAFGTAISFPLVESMLANAINDNERAKTMSILHVILFAVTAPFGYIGGILASMSEE
                     LPFVMMGGIFLICIFLIFILDRFKKPLEHPQEEPEL"
     gene            complement(3141..4925)
                     /locus_tag="VFD89_01960"
     CDS             complement(3141..4925)
                     /locus_tag="VFD89_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003412015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ56985.1"
                     /translation="MIKNRLIDDEKLEKPFNREQFVRLLRYMKPFTKQIIVTLILMIV
                     AAVSGLLGPYFLQIAIDDYIEVADFNGLTMITLLFLGSNLINMVCMKQRVYIMSSVGQ
                     RVLYRIRQDLFEHIQGLSFTFYDTRPAGKILVRIINDVNSLGDLLTNGIINVLTDFFT
                     LIIAIFIMAYINLRLTLVTLSTVPLLMILVGVLKRRIRRRWQEVRKKNSNMNAYLHEN
                     LAGMRVTQAFVREPETRQLYNELSGDIFTTWMGAIKLNNLFWPGIELIGALGVILVYI
                     YGIGSLDSNTVSVGVLVAFIGYVWRIWQPINNLANFYNSLLMAMASTERIFELLDIGP
                     DIKDEDDAYPIPEIKGEVRFDNLEFYYDSDKPVLKDVDFAVKPGETIALVGPTGAGKS
                     TIVNLISRFYDPIDGKVLIDGHDIKGVTLNSLRKQMGVMLQDSFIFSGTIMDNIRYGK
                     LDATDEEVMDAARVVNAHGFITEMEKGYHTEVKERGSRLSQGQKQLISFARALLADPR
                     ILILDEATSSIDTQTEVLVQEGLARLLKGRTSFVIAHRLSTIRNADRIMIVDEGTILE
                     SGNHDELIKQKGIYYNLYKAQYKFLNAV"
     gene            complement(4927..6678)
                     /locus_tag="VFD89_01965"
     CDS             complement(4927..6678)
                     /locus_tag="VFD89_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ56986.1"
                     /translation="MHIIKRILGYLGRYRGKIITAISLLILSIIFDMVSPYIYKLMVD
                     EVIRGGNRELFTTLLIAILVVAAARGSMQYLRGYFLEGVSQSCLYDLRNEMFTHIQGQ
                     SFTYFDDNRVGELMSRMTGDLEGIRSFIAGGIPILLENLIYFIGTSIVLFSMNIRLAL
                     VSLAVSPLVAWLAMRFDSRIRPVFSEIREQQAVLNTATQENITGVRVVKAFAREDHEI
                     QKFEKENRLNLDKNVKSAHIWAKYFPVMDFVSGICSVAVLWYGGRMVANGVISLGTLV
                     AFNSYLWMLIMPMRMLGWVINVMEQAVTSGQRVFDILDTGSIITEKDNPYEPEKVRGH
                     VVFEDISFHYKDQQALFDISLDAPAGKTVAIMGTTGAGKTSIINALNRFYDVSRGRIL
                     VDGVDVRDWSLNILRENIGIVMQDVFLFSATIEENIMYGNPNATRDEVIRAAEIADAH
                     DFIMEMPQGYETIVGERGTGLSGGQKQRIAIARAVIKNPKILIMDDCTSAVDMETEFK
                     IQQALKGIMEGRTTFIVAHRISSVKDADEIILLDEGRIIERGAHHHLLNKKGEYYNIY
                     SQQYKDFEDFEEDRRVI"
     gene            complement(6891..7823)
                     /locus_tag="VFD89_01970"
     CDS             complement(6891..7823)
                     /locus_tag="VFD89_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZJ56987.1"
                     /translation="MDMYNGFITDVSGIEVGHAQDIDAGTGCTAILCRQGAVAGVDVR
                     GAAPGTRETDLFRPSNLVQEVHAIMLSGGSAFGLAAADGAMKYLEEHGIGLDTGAARV
                     PIVGGAVLYDLAYRDSKIRPDFHMGYQACKVASGELPEQGSIGAGTGATVGKVLGMAS
                     AMKGGIGTSSITLPGGVVVGAIVAVNAFGDIFDLKNGEIIAGARDPKTGDFVNTWDYL
                     LSGGDKLAGFAGNTTIGVVATNAKLSKDQANRIASISHNGLALTIRPVHTMVDGDTMF
                     ALSTGEKAGDILAISCAAVEVTARAIVNAIRFSI"
     gene            complement(7810..8799)
                     /locus_tag="VFD89_01975"
     CDS             complement(7810..8799)
                     /locus_tag="VFD89_01975"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZJ56988.1"
                     /translation="MRNKVLKALLDNKDSFISGERISSSLGITRAAIWKHIKTLQHEG
                     FVIDTKPRLGYRLVKIPDILDETILLESIDTGTMGRNIEVHDTINSTNNRARELALAG
                     APEGTLIIAETQTVGRGRLGRNWISPKGRGVWMSLILRPDIPPEKAPGITTVIAVAIR
                     RALNQLTGLDFGIKWPNDIIMDGKKVCGILTEMHGDMDRIYYVAVGIGINVNLGKSDL
                     PAELIQTATSLNVGMGENMDRRSIIARAMKEIEDTYSRYIEDGDFDAILKECKMHSLT
                     LNRSVTVIGRDGEFEGYAIDFDADGSLLVKMQDGRVRKVISGDVSVRGRDGYV"
     gene            complement(8911..9858)
                     /locus_tag="VFD89_01980"
     CDS             complement(8911..9858)
                     /locus_tag="VFD89_01980"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HZJ56989.1"
                     /translation="MNRKKRGVTLVMAVTVILVAGLVLSTAMPVQAATYPVLRLGSRG
                     QAVSRLQQALKNGGYFTYPKITDYYGTITRDAVTRFQRDYGLYVDGIAGQKTQTALYG
                     GNTGTGYTGSLYFGMYNERVRDLQNALKGLGYFRGTATAYYGHVTENAVIAFQIDYRL
                     KIDGIAGPQTQGVLFGGKPAGNSTADRNALTAKQRDDIFWLARIIHAESQGESYTGKV
                     AVGNVIMNRVSSNQFPNTIYNVIFEYYYNIPQFSPVEDGTIYNNPSAECIKAAEEAYW
                     GSKPVGSALYFFNPRKAAGSWIVNNRQYITSIGSHAFYR"
     gene            complement(10049..10978)
                     /gene="pfkB"
                     /locus_tag="VFD89_01985"
     CDS             complement(10049..10978)
                     /gene="pfkB"
                     /locus_tag="VFD89_01985"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HZJ56990.1"
                     /translation="MITTVTLNPSIDKTITIEGFTYGGMNRIIGSRIDPSGKGINVAI
                     AYSQLGGQALCTGINYRENGNLIEQRLDRDDIAHDFVMADGEVRINHKVYDRSTKAVT
                     ELNESGHMVTDEVLAELKNKLVAHCLRSDILVLSGSAPKGVPTGIYKDLLGSVSDLSV
                     KTILDAEGDLLLHGLKGSPDLIKPNLFELETALKIKITSHGDIVKAAQVFLDKGVKVI
                     GVSLGAEGAVIVNESHAYYAPALKVDVKGTTGAGDSVIAGFCLAIKEGLDLKEMLRYG
                     VAAATASVIREGTLMCTRRDFESLLPKVQVNRL"
     gene            complement(11039..13120)
                     /gene="fusA"
                     /locus_tag="VFD89_01990"
     CDS             complement(11039..13120)
                     /gene="fusA"
                     /locus_tag="VFD89_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZJ56991.1"
                     /translation="MKNYETKEIRNIAIVGHGSEGKTTLTEAMLFNAGGTDRFGRVED
                     GTTTTDYDPEEIKRKISISSALAPLEWNNHKVNVIDVPGYFDFVGEMLGALRVVDSAV
                     LVVGAVSGLMVGTEKAWDYCESHVIPRMVFINQMDRENANFPKVMDQLKDKYGTSIAP
                     FQIPIMEGGEFKGFVDALRMNAKIFDGKELKDAPIPDSMMEEVEPIRTMLVEAAVETS
                     EELMEKYFEGEEITTEELTSAIRHGVLIGDIVPILCGSAVNNTGVKVLLDEVVNYLPS
                     PCDRPSFKGTNPRTEEVMRREMKVTEPFSALVFKTVVDPFVGKLSIFKVMSGELNSGA
                     TVYNASKDTNEKTTALYTLKGKKQNQVTKITAGDIGAFAKLQQTETGDTLCDATSPVV
                     YKGIEFPEPAISMAIVSEKEGEEDKVFGGLNRMVEEDPTFEIVTNPETNQTLISGVGE
                     MQLDVISSRLKAKFGVGALYKDPKVAYRETIRKPVKAEGRHKKQSGGHGQFGHVWIEF
                     EPLTDADTQFEFVDKIVGGVVPRQYIPAVEKGLQESLPNGVLAGYPMVGLRATLYDGS
                     YHSVDSSEMAFKMASYLAFRKLEQANPVLLEPIMKAEVTVNDEYMGDIIGDLNRRRGR
                     ILGMTPIAGGLQCIEAEVPQAEMFKYATDLRSMTQARGSFKLTFIRYEEVPGNISTKV
                     IEQAKMEREQQ"
     gene            13771..14877
                     /gene="aroB"
                     /locus_tag="VFD89_01995"
     CDS             13771..14877
                     /gene="aroB"
                     /locus_tag="VFD89_01995"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZJ56992.1"
                     /translation="MKPININLDLPINPYSIHIEKSWGGLEGALKPFIGHKTMLITDE
                     NVNNLFARDIASILSFMGCQVNVAVVKPGEASKSLEIAETLYTRALEANLDRSSSIIA
                     LGGGVVGDLAGFVAATYMRGINYVQIPTTLLAQVDSSVGGKVAVNHPLAKNIIGNFYQ
                     PKSVYINIDTLKTLPERQFSTGMAELIKYGFIWDNGFLKWLQSNIHDIMNRQEEGLAR
                     AVGCSCEIKAHIVSRDEREKGLRAILNFGHTVGHGLESLTNYDLYTHGEAVSLGMVYE
                     SSIACRMGLVDKSMVDLLIRLLDKANLPTEIHGVDTEALIKAMAHDKKNQGDSIVFVL
                     PIGIGKVDVFNNIDSGLIREVFKISPKDFLAEPW"
     gene            15234..16328
                     /gene="hisC"
                     /locus_tag="VFD89_02000"
     CDS             15234..16328
                     /gene="hisC"
                     /locus_tag="VFD89_02000"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZJ56993.1"
                     /translation="MVKQRNAINNLNPYIPGKPISEVQRELGLDRVIKLASNENPLGN
                     SPAASKAVKDWAQNMAIYPDGNSADLKAALSEKLGVGPEQLLFGSGTDQILEIIAQTY
                     INPGDNAIMGDPSFPRYETVVRVMDGEIIKIPLTGEHRLDLEGFLSKVDSRTKIIWLC
                     NPNNPTGTMITAKEQRDFIERIPGDVLVVLDEAYYEYAFGNDYPDSIGLLDEFKNLLI
                     LRTFSKAYGLAGLRVGYAISNSDTIGYLNRVRGPFNTNAAAQTAAVAALGDKDFIDKS
                     IANNVRGKEFLYSSFEGMGLDYIPTYANFLMVRVNLPSAHVFGELLKKGVIIRSGDIF
                     GMDDWIRVTVGTEEENNLFIKALGEVLAGK"
     gene            16694..17713
                     /gene="aroF"
                     /locus_tag="VFD89_02005"
     CDS             16694..17713
                     /gene="aroF"
                     /locus_tag="VFD89_02005"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZJ56994.1"
                     /translation="MVIVMKANATNEQIDGVEGFLTNLNLGVHISTGSERTIIGVIGD
                     KRILIDTSLEIMPGVDRLVPIMDPYKLVGKTFRPESTVVNIKGVEIGGTSLIMMAGPC
                     AVESEEQLMETATFLKKHGVQFMRGGAYKPRTSPYSFQGLGEEGLKLLARVREETGLL
                     IISEVVSNDSLEKALQYVDVLQIGARNMQNFDLLQHVGKSKMPVVLKRGLSSSIEEWL
                     NAAEYILSEGNYQVILCERGIRTFETATRNTLDLSSVPLIKEISHLPIIVDPSHGTGK
                     WSLVTPMARAGIAAGADGLIVEVHPNPQLALSDGNQSLNFESFSALMDEIRKIAPILK
                     RELPQ"
     gene            17710..18819
                     /locus_tag="VFD89_02010"
     CDS             17710..18819
                     /locus_tag="VFD89_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HZJ56995.1"
                     /translation="MTFHRICIVGLGLIGGSLAKAIKRSNPQSFITAVDSDQKSLDDA
                     FLDKVVDKVTADLYEAVEGAELIFICTPVHTISPQLERIAPVLDKDTIITDVGSTKQE
                     IMKAAGKIEAINQDGAVFIGGHPMAGTQHSGYEASLPHLFENAYYLLTPLDSTPAWAV
                     ESLKKLLLSIGALPMVMAAKDHDKIIGAISHLPHVVAAALVNTVAKLEDPSLLYMKLA
                     AGGFRDITRIASSNPEMWKSICMDNKEELVAHITGINRQLDTFTRMLLGDEETEVEDF
                     FASARAYRDNLPARQAHSLLSYYDLYVDVEDQPGVIGDVATILGKHLINIKNLRIINS
                     REGEPGSLIISLSDSTSLERARKVLGENGYDTFTK"
     gene            18942..20234
                     /gene="aroA"
                     /locus_tag="VFD89_02015"
     CDS             18942..20234
                     /gene="aroA"
                     /locus_tag="VFD89_02015"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZJ56996.1"
                     /translation="MDIVLSCGNRLRGTIHIPGDKSISHRGIIFGALARGTTRLGNFL
                     MADDCINTINIFRQMGIGIDIKDHSTVIVQGKGLYGLSKPDNLLDAGNSGTTARLLMG
                     LLAGQNFDSTLTGDDSLRSRPMDRVIIPLRQMGAQIEGIKGSNFLPITVSGKGATLKA
                     MDYTMPVASAQVKSSIILASLYAHKSSYIHQPAISRNHTEIMLGTFGGRIHTQGKTVT
                     TFPGAMLYGQDLVIPGDISSAAYFITAALLVPDSEIVLKNVGINPTRSGILDVYRAMG
                     ASIDVEEIHTGGQEPSANIIVKSSQLKGTVIEGSLIPRLIDELPIIALAATQAEGTTV
                     IKDAAELRVKESDRIDSTVAILKEFGADAEATPDGMIIRGPAPLSGTGLRPGSDHRAV
                     MMAAIAGLIAQGSTTIRDANWVNISYPGFFAQIEALRC"
     gene            complement(20297..20965)
                     /locus_tag="VFD89_02020"
     CDS             complement(20297..20965)
                     /locus_tag="VFD89_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HZJ56997.1"
                     /translation="MERFVPEIRGTLRRHMIEVPDSIRKATGIKIFGKRIKSLAFTTD
                     VAVIKNINADAIIAVYPFTPQPAITKAITMAADVPVFCGVGGGVTTGKRVVNLALDAE
                     FEGAIGVVVNAPTDNETIMAVRATIDIPIVVTVISEKTDIQSRLEAGASILNVSGAGK
                     TIDIVKRIRDEFPEVPIIATGGPNDETIIRTVEAGANAITYTPPTPGELFKISMEKYR
                     KELE"
     gene            complement(21424..22194)
                     /locus_tag="VFD89_02025"
     CDS             complement(21424..22194)
                     /locus_tag="VFD89_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZJ56998.1"
                     /translation="MRIGICRPIEEIKAVEGLGFDYIEPTVTGIVQMSSIEYKEALKK
                     IKDAGIGCETFNVLFPGDIRLIGPEADLCKIRDYLKDAFERIAGLGAQIVVFGSGGAR
                     RVPSDWVFEDGWKGLVEVAGLIGELALPYNITVAVEPLNKKETNIINSLAQGIKFVDE
                     VRHPNVKLLADFYHMRMEDEPMGVIGETTADILVHTHIAQGQTRTFPLDESEDIYNDF
                     FTNLGDIGYGGRVSIEAGTKDINKEGPASLALLKRLTS"
     gene            complement(22196..23356)
                     /locus_tag="VFD89_02030"
     CDS             complement(22196..23356)
                     /locus_tag="VFD89_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010272774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ56999.1"
                     /translation="MDKVRIGIVGIGNMGSAHAMYLSQNKVKNAELTALCDINPKRLE
                     WARDNIGNGVELFENSDSMYSSGLVDGVIIATPHYDHPSLVIEAFNNNLHVLLEKPAG
                     VYTKQVREMNDAANKSDRIFSIMYNQRTNPLYQKMKDLVESGELGELKRTNWIITNWY
                     RAQSYYDSGGWRATWAGEGGGVLLNQCPHNLDLWQWICGMPEKVQAFISYGKYHDIEV
                     DDDVTAYVEYPNGATGVFITSTGDAPGTNRLEITGDRGKLIAEDGKLTFWRLRVSERQ
                     FNREYKGGFGEPECWKCEIPISGEETAHQGITQDWVNAIVKGTPLLSPGIEGINGLTI
                     SNAMHLSSWTDGWVSIPLDEDLFYQKLKEKIQGSTFQKGTAGDKALDVEGTY"
     gene            complement(23523..24296)
                     /locus_tag="VFD89_02035"
     CDS             complement(23523..24296)
                     /locus_tag="VFD89_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019911137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZJ57000.1"
                     /translation="MQKPKIAAQLYTIRDFTKTPGELDKSMERIKDMGYNAVQVSGIG
                     PMEHQQVKDIMDRHGLTICATHIGFDSMKENLDDVINQHRLWNCKYVGVGSMPGEYQS
                     SKEGYRAFAKEASEIGKKLADNGLQFIYHNHHFEFIKFDGVLGMDILLNESDPAAFGF
                     EIDTYWVQAGGCNPVDWINKVGNRMQVIHYKDMAISKDVKQIMAEVGEGNLNWPDIID
                     ASIKNKIEWAAVEQDICQRDPFESLEISLKNLQKLGLDS"
     gene            complement(24317..25342)
                     /locus_tag="VFD89_02040"
     CDS             complement(24317..25342)
                     /locus_tag="VFD89_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ57001.1"
                     /translation="MEGFGVAIIGCGAIHRVHADAIMNNGMGKILWFVDLKEDRAKAS
                     AKEYGGGWCTDYREALKDPGVNIVHICTPHYLHSQMAIDAVNAGKHVLVEKPAAINLE
                     QAKAMAKAARDNNKYITINFQNRFNPTSIKAKEIIRSGEMGGVRGVKGLVTWFRDADY
                     YMESGWRGSFQTEGGGVLINQSIHTLDLMRWLVGEVESIQGNVSTRTLKDVIEVEDTA
                     EATLYFKNGARGIFYATNCYTDNSAVEVEIHMDKGMLRIYDNELIRVEDGKSEKLIDE
                     NSETSPYKSYWGKSHDTLIRHFYKNIMNGAGKAIIPVEEGMWTLQILDGIYKSSSTGN
                     RVMIDDM"
     gene            complement(25492..25659)
                     /locus_tag="VFD89_02045"
     CDS             complement(25492..25659)
                     /locus_tag="VFD89_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57002.1"
                     /translation="MDHGRREWHDYIKLLEDIPGDHYALIEFVKDDRVDQYLEDAKTL
                     RAWLGGRDFVH"
     gene            complement(25688..27703)
                     /locus_tag="VFD89_02050"
     CDS             complement(25688..27703)
                     /locus_tag="VFD89_02050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZJ57003.1"
                     /translation="MKFFRLRSIALILVVLTLVMSSCVGENGILPVRVDEIDSGNHAE
                     KIEDYKTDQKGEADQGEADLIPREVLFADPVRTSPSISPDGTRLAYRLYEDGYDTILV
                     RDLEQDRRDIVVQQPSTPGNYRFFWAGDNKHILILQDSLGDENYHLYASNIETGKIKD
                     LTPYDDIQAMVIEASSKRPDEVLIALNKDDPANHHVYRVGISSGSIELLERNPGNIQG
                     WMTDDKGDVRGALASNPDGGSDILVRDNRDNSWRTLISWDYESSNNSAPLGFTKDGGS
                     VLILDSRESNAGRLIRVDIETGENTVLIEDPDYDVNEVLIDDETYEIQGASFIKQRKE
                     WVFFNADTEKAFETISKLNDGDVSFVSGSEDDRVWLLAFTSDDEPLSYYIYDSEKDEG
                     NHLFDELPDLNDYTLAKTEPISYKSRDGLTIHGYITFPLGSEREDLPLILNVHGGPWS
                     RDIWGFDPEIQWMANRGYVVLQVNFRGSSGYGKDFLNAGNKEWGARMHDDLIDGVNWA
                     IDKGYADPKRIGIYGGSYGGYAALVGASFTPDVFACAVDLFGPSNLVTLLGSIPPYWT
                     PILDNYYRRVGNLKTEKEFLESRSPLFKVDQIKIPVLIAQGGNDPRVKQSESDQIVRA
                     MEEHGLDVRYLLFPNAGHGFSSPDDRNTFYTAAEKFLAEHLGGRWQE"
     gene            complement(27743..30424)
                     /locus_tag="VFD89_02055"
     CDS             complement(27743..30424)
                     /locus_tag="VFD89_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZJ57004.1"
                     /translation="MLRFIIVLLLILSLAGFSIKKYSDKTTLIFAVDLSNSTREVRGS
                     MADFMRRSMENKPAKYQTGIITFGKDTFVEQPLSFDMIFHGLETIPDPNYTDIGSGLQ
                     MASALMPDDTQKRVLLLTDGNENMGEAIQRAKSLSHQGIRIDGVFFDTEPGAEVQISS
                     LDVPTKLYEGEAYDITITIDSTINTDGNIRLYANRSLVGEQGIEILKGENRFIFKDTA
                     LEGGIKTYEAELDVKRDSIKQNNRIVTYTEVQGIPILALVEGQEGDGRELAGILESAG
                     MNYKIYDPSSLPKELEELRKFQGTILCNVSADDLGEEKINILDVYAKSLGRGILFTGG
                     DNSYALGGYMDTQLAEMLPVDLDLNHKAEVPDLGLVLVIDKSGSMADGQYGISKMDMA
                     KEAAIRSTESLRTMDYVGVVAFDSEALWVVNTQHPEDLDGIHEKILSIPAMGGTNIYP
                     GLSLAYESLRGLNTKLKHVILMTDGQSMPGDYDGMLDKMKKEGITLSAVAVGQDADQF
                     LLEELAKSGGGRYYFTDEFADIPKIFTKETRMASQSYIQNRTFFPTITEHSPIISGFK
                     EGFPPLDGYITTLSKNTATMALSSDTMDPILAEWQYGLGRVVAWTSDIRGAWTKNWLS
                     WDQTEEFWLGVISRILPFGDDSQGFIETSLHGNQGQISVKLDHNMKEDLDGEAIIIGP
                     KGKETIVPLEVTRPGKYEGNFGITMPGVYIVNTRHSKGGKIVNTLEAGLAVNYSPEYD
                     LRQSGSIDFLKEIVAQTGGRIIDNPEEIFREDPEPVWTQTGIWPWLLPIALLLFVLDI
                     AMRRLNIADLIGKHFKLAGDKAAKDQGHGGRIVRGENQKARGQRATEPGIEKERRSPK
                     TAGKNVHEGTSKDPEDEAPGFTTQLLKARKGDKRKRL"
     gene            complement(30534..32363)
                     /locus_tag="VFD89_02060"
     CDS             complement(30534..32363)
                     /locus_tag="VFD89_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZJ57005.1"
                     /translation="MKFLNPWGFLFFISIPGIIILYLLKQQNQEMMVSSTFLWERTMQ
                     NLQASVPWQRLRANILLILQLMALVLFSLGISQPFLYSREEKDDYIIILDSSASMQAT
                     DIKPSRFQRAKSDIEKLIDGMLPHQRMTLVEASDRARILANQVSDKATLKAKLGQIKE
                     TNGFANMVEAFEFVRALAKEEPNSGIYLYSDQYHKAGDNEDNPYNTIIYNDNGQNRAV
                     QSVTYSYDSNTPIILSAITNYGSDSMVSVECLADGRLIDVKEVSLSEGETKNIYWDNI
                     PTKAQRIEIHILDEDDLMADNRAWAILSPPSVNRVLLVTTRNTFLEKAIGLRGDLELD
                     KTTLDGARDIGGYDLYVYDGYTPPELPEDGSVILFNPVGTGDILNIGDIFNPGAIRLN
                     SRTSYRDLVNYITPEDFHIAEARILEKPPWAEMVLEDEKQRPIMMVGQLENQRIAIFP
                     FEIHKSDLPLKSDFPILMQNLLGWMLPQIVDYDAERFAGDEVEINPFPHAQKVTVENP
                     YGLTMDVGPPFPIPPFSETQEVGVYKVRQRIDEKDIEGYFTVHIPTLKESDLRLIDQP
                     STETKIQTDDRLRLSTGKSVWHYFIWAVLILILAEWWVYQRGY"
     gene            complement(32360..33244)
                     /locus_tag="VFD89_02065"
     CDS             complement(32360..33244)
                     /locus_tag="VFD89_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZJ57006.1"
                     /translation="MKKTPFDQDFLNKLNRLSLASKMAVKGSTGGQRKSNLKGDSVEF
                     SDFKEYTPGDDFRRIDWNAYARFERLFIKLFMEEQQSQITIILDCSGSMDFGDPNKGF
                     LAKQLAGIFCYLGLNTLDRVGLLCINDKVEDRLPYFTGKQGFWKALKFIEEIPFGGRT
                     SLNTAIKMCQSIGSRGGTTIILSDLFSQDGYEEGIKYLQYQKQEISLIHILSPEELDP
                     PWDGNIRLVDKEDQNYRDINVTQQVMRAYRDTLDGFLRDVREFCYNRGVHYVPINSGT
                     GIEGIVFDNLVKMGVISS"
     gene            complement(33262..34245)
                     /locus_tag="VFD89_02070"
     CDS             complement(33262..34245)
                     /locus_tag="VFD89_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZJ57007.1"
                     /translation="MEITQDTIKSFARQVEAIEKEIGREMIGQKEIIRQTLMTIIAGG
                     NALLEGVPGLGKTRLVRTIGNVLDLEFSRIQFTPDLMPADVVGTNIIARDRDGNSRFE
                     FQPGPIFGNIVLADEINRATPKTQSALLEAMQEHTVTVGRDTRKLPQPYFVLATQNPL
                     EQEGTYPLPEAQLDRFFFKLMVKFPSLDELDGIVNLTTMGEEKKADKVAQDETILQLR
                     SIAQQVPIAKGVQNYALRLVLATQPEGENASTAAQKYVRYGSSPRGAQAIISAARIRA
                     LMEGRYNVSFDDIRYVAYPALRHRIILNFEGIAEGRNPDNVLEEIIGELGE"
     gene            complement(34442..36127)
                     /locus_tag="VFD89_02075"
     CDS             complement(34442..36127)
                     /locus_tag="VFD89_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57008.1"
                     /translation="MSRGVDSKIHRAFGPIRRRLTAQHLIHGVIWGSIAAIGQGILWM
                     GVSFLIPIIHIKSIMAYTGGIVMAITFLVSWVTRPSISKMTKKADKLGFEERITTAYE
                     LSHRDDQFAKAQRHDTLNRLKAFDPKNIDITTSKKSSLIAMGLLMLLMSGFFIPNPQD
                     GIIKKRLHAEKVIEEQLKRLEDESNKELAMEAGLTPKEREEIKKLLARLTDKLKKTRD
                     HREALKEISKTQEEIIELAKETKRQEMADLGEGLSRQEALKSLGEQIKEMDTEGIKEE
                     MGRLQELAKNGGMDKDAMDAVKDALEQMADKLGKGKIQDRLMELAGDIGKNLDKDTMD
                     LAEELGVLEDLLMDLAGGPLSETQDLMYALQDMKNKISKAGSVDIDKSQLAQSNGPDN
                     QEGDSGNSNADQGNSQGNIEEPGQGQGSQGGQQGQGSQSGQQGQGSQSGQQGQGNQSG
                     QGQGGQGGSDSNSAGGGQGAGIGTAHPEYERILDPKRLGDGGEVSQVKGNINEQGSGQ
                     QIETGQGLGDFDGFVPYDEVFGEYKNQAMRNIERMSIPPNMREWIKSYFTALE"
     gene            complement(36159..37133)
                     /locus_tag="VFD89_02080"
     CDS             complement(36159..37133)
                     /locus_tag="VFD89_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZJ57009.1"
                     /translation="MKKWLYNPVMVSELKIKMRGWRTAAAVTVYLGIMLLISYLFFNL
                     TFDNTYGYGNTFDVNNSIGLQIYTTLAILQFALIVLITPAQTAGSISGEREKQTLDLL
                     LCTKMSPTGIVMGKLISSMSFIFLLIISSIPIFSLALLFGGVTPGDIVVLFAFYMITA
                     FAIGSIGMFCSVLFKKTVTSTVISYLTIFVLGILTAIIGATMLQSYYYNSSSVSGMTL
                     DSYTPFVFYLNPVTGLADILARQSGSGGNLGVAGVLSMMFGMGYYGTSNPNTGGMING
                     WSWSQWSLWIRNAVIMLSAAGTLLTISAWKIKPVRRGFRKKHLANISK"
     gene            complement(37114..38046)
                     /locus_tag="VFD89_02085"
     CDS             complement(37114..38046)
                     /locus_tag="VFD89_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016201088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ57010.1"
                     /translation="MLVIKDLVKRYGKFTAVDQVNLKIESGSIFGFVGPNGAGKTTTI
                     KIIATLMQPTSGTVEVEGMDVVREPLKVRERIGYMPDFFGVYDNLKVHEYMDFYGSSY
                     GMPYKERQKAAGELLELVDLGHKKDSYVDTLSRGMKQRLCLARSLIHNPELLVLDEPA
                     SGLDPRARIEIKGILKELKGMNKTILISSHILSELAEMCDTIGIIENGKMVIDGSVED
                     IMRRISGRTIIRLKFMDRLEEAINLLKEQPLVEEIIQEGNSLEVTYSGEDQDLWKLLR
                     ILVNQELPILSFGKADGNLEQIFMEVTKGEEMAL"
     gene            complement(38046..40505)
                     /locus_tag="VFD89_02090"
     CDS             complement(38046..40505)
                     /locus_tag="VFD89_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57011.1"
                     /translation="MNNRGIILVCILMIVLTIIPMGILAEGEEDQSQREDIIIEAKIG
                     FDNHYRVGSWTPIVIEIENLGQDIHGDLEVLVNQDQGTAITYSRAAVIAKNSKKRFML
                     YAQINTTQRKFKIDLKQKNKTLKSLELKSLKPMTLNKYLMGVITDDKPALGYWWRTLG
                     GDSVFANHETVLINKDEIPHRPEVMENFAMIVINNADTAVFTQEQQQVLGQWVNNGGI
                     LIIGTGSNASKTLSGLKPTIVDGQIGNTIKSEPIEGIGNMSLDLAGLSLQDSKTLMED
                     GGQAIIWGLNSGRGNIFIAGFDLGLEPFTSWEGNKTFWERVLTNNLKPSVIATLKGPD
                     LSSSGYQPYGYGYNYGIREALSSIKALDLPPFTNLLIILLGYLLVVGPINYIILKKLD
                     KREWMWFTIPGFVILFSIGIYGIGYSQKGSEVIINTISTISARENTATIDTYVGVFIP
                     KRGDYNISIDGDVLMTPYGEGDMGYAYGSSFPSSHGGIIKAKVSQGNPSSITLYDSNV
                     WTMRTLMYRGFKENLGDIKGDLYYKDGKVKGTIINNTSFLIEDSAIYTLAGDFEKIGN
                     LIPGVEVEVNLSVSSPSTQSGYGHNDLYMMIDSLFPYPNSGSRQPSDEERSRWAKRNF
                     VEGLIMKYDGNDLLHNDFINLLGFCDYDLEEKITVVGSGPVTPQNTGVVLSGFDIVYE
                     RDGQVGIPPGYVKGILKKELSDMIHSDDSIFYLDRGKGVFEFDMTPYMDLELTKIEVV
                     ARPYGDPIGLSPLDAGEGDYMDEDDSITYHKARGAIIISPDSISRFIDDEGKMLIKIE
                     GRPREYISFEAPTLIIEGRKR"
BASE COUNT        10524 a        10252 c         8251 g        11905 t
ORIGIN      
        1 catcataata acatgaaatg gatattttgt aaattttaca gtttcatggc tttagtccgg
       61 atcccacgcc attatttaat ccgggggatc ctcggctatg cctaacgact ctgatttctt
      121 tcgtatatta tcttaaggcc ccttagtgtc aggttttcat cgatttcatc tactgtttct
      181 gaatcccggg ctattaaggt agctaatccc ccggtagcta taacctttac gttcggggca
      241 tttaattctc ttttcatacg acggactata taatcgacct gcccgatgta accatagaca
      301 atgcctgcct gcatactctc aactgtgttc ttgcctatta tattggttgg cttaatcagt
      361 tcgattctag gcagctttgc tgcatattga aataaagcct cagtggatat cttaattccc
      421 gtagaaatgc atcctccaag atactccccc tgggctgaaa ctgcacaaaa tgtagtcgct
      481 gtgccaaagt caataatcac caaggggctc ctgtaggttt cataagctgc aatggcattg
      541 actatcctat ctgcgcccac ctctttaggg tcatcaattc gaatattcat tccggtttta
      601 attcccggtc ctattaccag aggttcaata tcaaaatact ttttgcacat caattccaaa
      661 gcatccatta aggtagggac caccgaagat atgataatcc cgcttatatc atcctcagat
      721 atcctgctat atctaaacag gtccagcacg agaataccta attcgtcgcc tgtcttatcc
      781 ctatccgtcg tcagtctcca acttgttacc aaatcctgcc catcatacac ccccaggact
      841 acgttggtat tgcctatatc gattactaag atcatggcct atagatttcc ctccctgcat
      901 aagtatgggc agggaaacct ccctgcccgc agcagcttat caaatcgctt aaaaatcgcc
      961 cttataaaac tttctcaatg cctgaactat tggtgttata atcagcacgg cagcgattgc
     1021 ttctggaacc ccgttgggta tcgcaatgcc gtatatcaca gctttaccta ccatcgattg
     1081 ttcaacattc aacatcttcg caaaaacgtc cttgtataat aggtaggcta tgcccagtac
     1141 cccaatggtg tttgttaaag ttccaacggc agaggctacc atagtactta gggctattcc
     1201 cctttgacct tttcccctca aaaggttttt tatgccctta aaggaataat agctggttaa
     1261 tgcgatagct agcctaggca gtattgaaac cagggggttc ataaatgcta ccgacaagac
     1321 gctcggctgg gtaaaggccc tatacatact gaatccgcca aaaattaagc ctataaacag
     1381 ccctactaca gggccctcga ctatggcacc tatgatggtg ggaatgtgca taatagtgac
     1441 ttgcaccggc agaaagggaa gctgaattat cccaaccccg ccaaattcca gcattataga
     1501 tatgccacct aacattccaa taactgctaa tttacgtgtc gtgattttca attgacatac
     1561 ctccgttcaa gttcctaatg cggatacctg acagcatcaa cgtattagat tttaattcta
     1621 tagtccaact ccttcgggat gtcagctata gatttatggt aattatcata tcaaaaccgt
     1681 gccggaaatt caagggattt tttataattc cggctcctct tgcgggtgct caaggggttt
     1741 tttaaaccta tccaagataa atattagaaa tatacagatc aaaaagattc cgcccatcat
     1801 aacaaagggc agttcttcgg acatagaagc tagtatgccc cctatgtacc caaagggcgc
     1861 cgttacagca aaaagaataa catgcaggat ggacattgtt tttgctctct cgttgtcatt
     1921 gatggcatta gccagcatgg attctaccag gggaaaggat atggccgtcc cgaatgccat
     1981 aaatatagta gaaataatta acacggtata gctcctattg ggggaaacaa ccaggagtag
     2041 gttgcttata atcagagata taaaggagcc taggaggggt ttttttatgt tggtttttcc
     2101 caatatgggc attacgaata gcagaactaa aagcattatc cccgattgta tcatagggaa
     2161 aagggctata ctcccttggc tgaactccag ctttttagtc agtagtatag caaaaaagtt
     2221 ggtccttata acggtgtgga tattggtaaa aatggataat aggaatgcaa tcatggtata
     2281 gggcgcagcc aataactgcg aagcagccct tttataatcc ttaaggctat caaagaatcc
     2341 cccattttcg gactccttca tcctgatgag cccctgggca gtctcgtggg ccagaaaatg
     2401 tctaagaata aacataaagg tcatggagat gaaggcaaaa aaataaagcc ctctcacagc
     2461 gggtacaagc cggtatttgg acacaaaaaa accggcaaca ggtgcaacaa atccagataa
     2521 tatccctgcc acctgcatcc aggtaaatac gtggacccta tcctccgatg atgtatcctc
     2581 tacaaacagg caggtccagg aggtatgcac taccctaaca gcactgttaa ttatggcagc
     2641 agcgagaaaa taatagaagt tctgggctat ggcccaaata aaggtaggga cgctccaact
     2701 gataagatca aatatcagac tggtacgctt tctgccgagt ttgtccgtta tatgaccgct
     2761 aaccaaggag aaaaacattt gaaacatcag gcccacagaa gcaataaggc ctatttcctt
     2821 ctcggtacat cccaatgccg ccatatatac ggaagcataa gtaataaata gattataggg
     2881 tataccccat aggggttccg tcataatgca gaccctagcg tttcccttaa agccctttaa
     2941 aatgtttccg gtctcccgta attgacccct taactttccc tcaaccatac atccaacccc
     3001 caaaatgtca tataatcacc gatgcaaaat acaccggtta caattatagc ataaaaggct
     3061 tatgctggcg atatgtaaac ttggcctttg gaaataattc cggagccata tttgatggca
     3121 ccgggatcgt aaaagctagg ctataccgca ttcaaaaact tgtactgggc cttgtataga
     3181 ttatagtaga tccccttttg cttaatcagt tcgtcatggt ttcccgattc taatatggta
     3241 ccctcatcca cgatcataat gcgatccgca tttcttatgg tggatagcct atgggcaatg
     3301 acaaaggatg ttcttccctt caacagccgg gctaacccct cctgcacgag gacctcggtc
     3361 tgagtatcga tgctggatgt ggcttcgtcg agaatcagta tcctaggatc tgccaataaa
     3421 gccctggcaa aagagatcag ttgtttttgg ccttgggaga gtctagatcc ccgctccttt
     3481 acttccgtgt gatagccctt ttccatttcg gtgatgaaac catgggcatt tacgacccgg
     3541 gcagcatcca taacctcctc atcagtggca tccagcttcc cgtatctgat gttatccata
     3601 attgtgcctg agaagataaa gctgtcctgg agcatcacgc ccatctgttt tctaagggaa
     3661 tttaaggtga caccctttat atcatgccca tctataagca cttttccgtc aatgggatcg
     3721 tagaaacggc ttatgaggtt tactatggtg cttttccccg cccctgtggg gcctaccaag
     3781 gctatagttt cgccgggctt tactgcaaaa tcaacatctt ttagaaccgg tttatcggaa
     3841 tcataataaa attccagatt atcaaatctg acctcgcctt taatttcggg aatgggataa
     3901 gcgtcatctt catcctttat atcaggccct atatccaaca gctcaaatat cctttcggta
     3961 gaggccatag ccatcaggag ggagttgtag aaattggcca ggttgttgat gggctgccat
     4021 atcctccaga cgtatcctat gaatgcaacc agtactccga cggagacggt attactgtct
     4081 aatgaaccta taccatatat atataccaga attacaccca atgctccaat caattctatg
     4141 cccggccaaa aaaggttatt aagctttatg gcacccatcc aggtggtaaa aatatccccg
     4201 ctcaattcgt tgtataattg cctggtttca ggctccctta cgaaggcttg tgtgaccctc
     4261 atccccgcta ggttttcatg aagataggcg ttcatattgg aattcttttt tcggacttcc
     4321 tgccatctac gccggatccg tctttttaat acgcctacca ggatcatcaa taagggtact
     4381 gtgctcaatg taaccaaggt caatctgagg tttatataag ccataatgaa tatggctatt
     4441 atcaaggtaa aaaaatctgt caatacattg attatcccgt ttgttaaaag atcccccaga
     4501 gaatttacat cgtttattat gcgcacgagt atctttcccg caggcctcgt gtcataaaag
     4561 gtaaaggaca gaccctgaat atgctcaaaa agatcctgtc ttattctata tagaaccctc
     4621 tgccccacag aggacattat atagaccctt tgcttcatac ataccatatt gatgaggttg
     4681 ctccctaaaa ataacagagt gatcatggtc agtccgttga agtccgcaac ttcaatataa
     4741 tcatcaatgg caatctgcaa aaagtacggt cccaataatc cgcttacagc agcaactatc
     4801 atgagtataa gggtgacaat tatctgtttg gtaaagggtt tcatatacct tagcaacctt
     4861 acaaactgtt ccctgttaaa cggcttttcg agtttttcat cgtcgattaa tctgttcttg
     4921 atcatcctat atcacccgcc tatcctcttc aaaatcctcg aaatccttat attgctggga
     4981 atatatgttg taatattcgc cctttttgtt taagagatgg tgatgcgcac ccctttcgat
     5041 tatgcgaccc tcatcgagta atatgatttc gtcagcatct tttacggagg atatcctatg
     5101 ggcgactata aacgtggtcc ttccttccat aatgcccttg agggcttgct ggatcttaaa
     5161 ttcagtttcc atatccacag ccgatgtgca atcatccatg ataagaattt tagggttctt
     5221 aattacagcc ctggcaatag caatcctttg cttttggccc ccggataaac cggtaccccg
     5281 ctctccgacg attgtctcat agccttgggg catctccatt ataaagtcat gggcatccgc
     5341 aatttcggcc gctctgataa cctcgtccct tgtggcattt gggtttccat acatgatgtt
     5401 ctcttcgatg gtagctgaaa agaggaagac atcctgcatg actatgccta tgttctccct
     5461 gaggatattg aggctccaat ccctcacatc aaccccatcc actaaaatcc ttccacggga
     5521 aacgtcataa aaacggttta aggcgttaat tatagaagtt ttaccggcac ctgtggtccc
     5581 cataatagcg actgtctttc ccgccggcgc atccaggctg atatcaaaga gtgcctgttg
     5641 atccttgtaa tgaaaggata tatcctcgaa tacaacatgg ccccggacct tttcaggctc
     5701 atatgggtta tccttctcgg ttataatgga gcccgtatca aggatatcaa agaccctctg
     5761 tcccgaggta accgcctgct ccataacgtt aataacccag cccagcattc tcataggcat
     5821 tatgagcatc cacagatagc tgttaaaagc caccaaggta cctagggaaa taaccccatt
     5881 ggctaccatc cgtccgccgt accataatac ggcaaccgaa cagatccccg agacaaaatc
     5941 catcacagga aagtatttgg cccagatatg ggcagacttg acgttcttat caaggttgag
     6001 cctgttttct ttctcaaact tttggatctc atgatcttct ctagcaaagg ccttaactac
     6061 cctgacgccc gttatattct cctgggtcgc agtgtttaaa accgcctgct gttctctgat
     6121 ctcactaaag acaggacgaa tgcgactatc aaatctcatg gcaagccatg ccacaagagg
     6181 cgataccgcc aggctcacca aagccagcct aatattcatg gaaaacaaca ctatagatgt
     6241 tcctataaaa taaatgaggt tttcgagaag aatgggtatt cctccggcaa taaacgatct
     6301 tatcccttcc agatcgcctg tcattcttga cataagctca cctacacgat tatcatcgaa
     6361 ataggtaaat gattggcctt gtatatgggt aaacatttca tttctgaggt catataagca
     6421 gctttgggac acaccctcta aaaaatagcc ccgaagatat tgcattgatc ccctagcggc
     6481 tgcaactacg agaatagcaa ttaaaagtgt tgtgaatagt tcgcggttcc ccccacggat
     6541 gacctcgtca accataagtt tatagatgta tggagaaacc atgtcgaaaa taatggataa
     6601 aattaaaaga gatatggcgg ttataatctt tcccctatat cggcccaaat agcccaatat
     6661 ccgtttgata atatgcaaaa aataaacccc ctctttgcct tttcccggat tctacaatat
     6721 attaattcac ttttctacat tggtcaacga acaaaaccag gtgttatcta ttgtattcac
     6781 ccttgtaatc cacggaaggt aaacccttcc gatacacctc caaagtttag caaattggtg
     6841 ggattaccta tgtattcctg ccaagggata taaagaagta tctttatctg ctatatggag
     6901 aatcgtatgg cattgacaat ggcccgagct gtcacctcca ccgcggcaca actgatagcg
     6961 aggatatcac ccgctttttc acctgtggac agcgcaaaca tcgtatcccc gtccaccatt
     7021 gtatgtaccg gccttatggt aagggccaat ccgttatggg agattgaagc tattctattg
     7081 gcctgatcct ttgacagctt tgcgttggtt gctacaacac caatggtggt attgcctgca
     7141 aatccggcga gtttatcgcc accggacagc agatagtccc atgtatttac aaaatcaccg
     7201 gttttcggat ccctggcccc ggcgattatt tcgccgtttt ttagatcaaa aatatcccca
     7261 aaagcgttta cggcgacaat ggccccaacc accacgcctc cgggcaaagt aatgctggag
     7321 gttccgatac cgcccttcat ggcggaagcc atccccaata ccttcccgac cgtagcgccg
     7381 gtccccgcac cgatgctgcc ttgttctggc aattctcccg aagcaacttt acaggcctga
     7441 tatcccatat ggaaatcggg tcttatttta gaatccctgt aggcaaggtc atataatacc
     7501 gcacctccta ctatgggaac ccgagccgca cctgtatcga gccctatgcc atgctcttct
     7561 aaatacttca ttgccccgtc tgctgcagcc agcccgaaag cgcttccccc ggagagcata
     7621 atagcatgaa cctcctgaac taaattgctg ggcctaaaaa ggtctgtttc tctggtaccc
     7681 ggggccgctc ccctcacatc gacgcctgca acagcacctt gcctacacag tatcgctgtg
     7741 catcccgtcc cggcgtctat atcctgcgca tgtcctacct ctattccgct gacatccgta
     7801 ataaagccgt tatacatatc catctctccc cctgacagaa acatcgcccg atatgacctt
     7861 tcgaactctg ccgtcttgca ttttcaccaa taaagaaccg tctgcatcga aatctatggc
     7921 atagccttca aattccccat ccctaccgat caccgttacg gacctgttta gggttaggga
     7981 atgcattttg cactccttaa gaatggcatc aaaatccccg tcctcaatat atcggctgta
     8041 ggtatcctct atctccttca tggcccgtgc tattattgac cttctgtcca tattctcgcc
     8101 catccccacg ttcagtgatg tcgcagtctg aatgagctcc gcgggaaggt cggatttccc
     8161 tagattgacg ttgattccta taccaaccgc tacataatag atcctgtcca tatccccgtg
     8221 catttccgtt agaataccgc ataccttttt cccgtccata attatatcgt tgggccactt
     8281 aatcccgaaa tctaaaccgg tcaattgatt taaagccctt cttatagcta cagctataac
     8341 ggtggtaatc ccgggggcct tttctggcgg aatatccggc cttaatataa gggacatcca
     8401 tactcccctg ccctttgggg atatccagtt tctgcccaaa cgccccctgc ctaccgtttg
     8461 ggtttccgca attattagtg ttccctcggg cgcacctgca agggccagct ccctcgccct
     8521 gttgttagtg gagtttattg tgtcatgcac ctctatattt ctacccatgg tgccggtatc
     8581 gatggattct aagaggatag tttcatccag gatgtcgggt attttcacca gcctatatcc
     8641 caatctgggt ttggtatcta ttacaaaacc ctcatgctgt agggtcttta tatgcttcca
     8701 tatggccgcc ctggtgatcc caagggaaga acttattctc tccccggata tgaaactgtc
     8761 tttgttatcc aacaacgcct ttaatacctt gttgcgcata ataccctctc cctgatcttt
     8821 ggctgtccat ttcccatcta ttatatccat ttcggaatct attgtatagg cataaaggaa
     8881 aaaccccgca ccttatagtg cggggttttt ttaccgatag aaggcatggc tgcctatact
     8941 ggtaatatat tgtctattgt tcactatcca gctgccggcg gcctttcgtg gattaaagaa
     9001 gtataaggcg ctgccaacgg gcttacttcc ccaatatgcc tcttccgccg ctttaataca
     9061 ttcggcgctg ggattgttat atattgttcc gtcctctacg gggctgaact ggggtatatt
     9121 ataataatat tcgaatatga cattataaat tgtattcgga aattggttcg aacttaccct
     9181 gttcattatg acattcccca ctgctacctt ccccgtatat gattcgccct gggattcggc
     9241 atggataatc ctagccagcc aaaatatatc gtctctctgc ttggcagtta aggcattcct
     9301 gtcagcagtg ctgttaccgg cgggctttcc gccgaataga acaccctggg tctgtggacc
     9361 tgctatccca tctatcttga gcctatagtc tatttggaaa gcgattaccg cattttctgt
     9421 aacatggccg tagtatgcag tcgctgtacc cctgaaatat cctaggcctt taagtgcatt
     9481 ttgaagatcc cttacccttt cgttatacat tccaaagtac aaggagcccg tgtagccggt
     9541 accggtattt cctccgtata gggctgtctg agtcttttga ccggcgatgc cgtcgacgta
     9601 gagcccgtaa tccctttgaa atcttgttac cgcatccctg gttatggtcc cgtagtaatc
     9661 ggtaatcttg ggatatgtaa aatatccccc gttcttaagc gcttgttgca accgtgaaac
     9721 cgcctgtccc ctacttccga gcctgagtac aggataagtc gcagcctgaa ccggcattgc
     9781 cgtgcttaaa actaaacctg caaccaatat cacggttact gccataacca atgtaactcc
     9841 ccttttcttt ctgttcacag aaatccccct ctatatgcat tatgttaagt taccataatc
     9901 cattataagg gggggtagtt tctcggtcaa acccctgggt ggctgtgttt ctgttttatt
     9961 acaatattgt gacatgaatg ttaactatta ttaatttttc cttactgttt catggttata
    10021 ccaattttat ccataactat accccatatc atagtctatt aacctggact ttgggcagta
    10081 gactttcaaa atcccttctc gtacacatca gggtcccttc cctaatgacc gatgctgttg
    10141 ctgcggctac accgtatctg agcatctcct tcaaatccag accctctttt atagcaagac
    10201 aaaatccggc tataacggaa tctcccgcac cggtagtccc ttttacatcg accttaagtg
    10261 ccggtgcata atacgcatgg ctttcattta ctataacggc gccctccgcg cctaacgaaa
    10321 cccctattac ctttacgcct ttatccagga aaacctgtgc cgccttaacg atatccccat
    10381 gggaagtaat cttaatcttc aatgctgttt ccagttcaaa taagttaggc ttgattaaat
    10441 ctgggctccc ctttaaacca tgtaacaata gatcaccctc ggcatctaat atggtcttaa
    10501 ccgaaagatc ggataccgat cccaataaat ccttatatat accggtgggg acccctttgg
    10561 gggcgctgcc gctcaatact agaatatcgc tacggagaca atgcgccaca agcttattct
    10621 taagttctgc tagtacttca tcggtcacca tatgtccgct ttcgttcagc tcggtaaccg
    10681 ctttggtaga tctgtcatag accttatggt ttatccttac ttccccgtct gccattacaa
    10741 aatcatgggc tatgtcatcc ctatctaatc gctgctctat cagatttcca ttttccctgt
    10801 agttgatgcc tgtacataga gcttggccgc ccagctgact ataggcaata gccacgttta
    10861 ttccctttcc cgaaggatca atccgactcc cgattattcg gttcattcct ccgtatgtaa
    10921 aaccctctat ggttatcgtc ttgtctatgg atggattcaa agttacagta gtaatcatac
    10981 cgatcccctt cttacaattt aaaaagccgg gaaccccaag gcgcccggct cttatctctt
    11041 attgctgctc tctttccatt ttagcctgtt cgattacttt tgtgctgata ttccccggta
    11101 cctcctcgta ccgaataaag gtcagcttaa agctcccccg ggcctgggtc attgagcgaa
    11161 gatcagtggc atatttaaac atttcagctt ggggaacctc cgcttcgatg cattggagtc
    11221 ctcccgctat tggtgtcatg ccaagaattc tgcctctacg gcggtttaag tcgccaataa
    11281 tgtcgcccat atattcatca ttgactgtta cctcagcctt cataatgggc tctaatagta
    11341 ccggattggc ttgttccagc tttctaaagg cgagatatga tgccatctta aaggccattt
    11401 cggaagagtc cacgctgtga tacgatccgt cgtataatgt tgctcttaat cctaccatgg
    11461 ggtagccggc caatacgcca tttggcaaag attcctgtag acctttttca acagcaggaa
    11521 tgtattgtct ggggactaca ccgccaacta tcttatcaac aaattcaaac tgtgtatccg
    11581 catctgtcaa gggttcgaat tcgatccata catgaccgaa ctgtccatgg ccgccgcttt
    11641 gttttttgtg tctcccctct gccttgacgg gttttcttat ggtttccctg taggcgacct
    11701 tgggatcttt atacaacgct ccgacaccga acttagcctt caatctgctg gagataacat
    11761 ccaattgcat ctcccctact ccgctgatta aggtttggtt ggtctccggg ttggttacta
    11821 tctcaaaggt cggatcttct tctaccatcc tgttaaggcc tccgaagacc ttgtcctctt
    11881 ccccttcttt ctctgacact atagccatag aaattgcggg ctccggaaat tctatgccct
    11941 tgtatactac aggcgaagtt gcatcacata gggtgtcccc ggtctcggtc tgctgcaatt
    12001 tagcaaaggc cccgatatca ccggcggtaa ttttggttac ctgattttgt ttttttccct
    12061 ttaaagtata tagggcagta gtcttttcgt tggtatcctt gcttgcattg tatacggtag
    12121 caccgctgtt cagctcaccg gacatcactt taaatatgga taacttaccc acaaagggat
    12181 cgacaacggt tttaaatacc aaggctgaaa agggttctgt gaccttcatc tcacgcctca
    12241 tcacttcttc agttctagga ttagtcccct tgaaagaggg cctgtcacat ggtgacggca
    12301 gataatttac cacctcgtcc aacaatacct taaccccggt attattaacg gcagaaccgc
    12361 atagtatggg aactatatct ccgatcagga caccgtggcg aatcgcgctt gttagctctt
    12421 cggtggtgat ctcttcacct tcgaaatatt tttccatcaa ttcttcgctg gtttccaccg
    12481 ccgcctcaac caacatggta cgaataggct ctacctcttc catcatcgaa tcaggaatag
    12541 gggcatcctt caactcttta ccgtcaaata tcttagcgtt catcctcagg gcatcgacga
    12601 agcctttgaa ttctccgcct tccattatgg gaatctggaa aggggcgatg gatgttccgt
    12661 acttatcctt tagctggtcc attacctttg gaaagttagc attttcgcgg tccatttgat
    12721 tgataaatac cattctggga atgacatgac tttcacaata atcccatgcc ttttctgtgc
    12781 ctaccattag accggataca gccccaacca caagcacggc actgtcaact accctcaagg
    12841 cccccaacat ttccccgacg aagtcgaaat atcccggaac atcaataaca ttaactttat
    12901 gattattcca ttccaatgga gccagtgcgg agctgatgga aattttcctt tttatttctt
    12961 cgggatcata atcggtagtg gtagtcccat cctcaacgcg accaaagcgg tctgtaccac
    13021 ccgcgttaaa tagcatggct tcggtcaaag tagtttttcc ttcactgccg tgacccacaa
    13081 tggcaatatt gcgaatttct tttgtctcgt aatttttcat gcataatccc cctctttaat
    13141 aatattgcta tatccctaat cccaggaaaa tcggaaatca atatatggca tccggcagat
    13201 aatccctgct cataagccag ttagaaagcc caaaaaacat attgttcacc aggctaggat
    13261 aaaatcctaa cagactaagt tttttgatac tccggctaac caattgcttt ttttgatagc
    13321 gcctttaaag taaaaggaca aattagagct ctcaatgaac agctgatatt gaaataagtg
    13381 tttaccggga tagttaacca taaaatccat cggccgcgta taatgaaata acaaaactgc
    13441 cagtttggtt acaattgcct attggtttga tcatataatt tatatgtacc aatagaaatc
    13501 agaatttatc tttaagagca ccggaaacaa ttacttcagt tacgttatat tgtaacataa
    13561 attttaattt tgcgcactaa agattttaga ttaaggtgac agcatggccg gttgtacaac
    13621 ataaaaatgt tatggaaatt ttatggtatc tcattcacct tcgtattcct tgacttttat
    13681 gaatattgag tatagaatca actaaaaatc atcctttgtt tccgccatgt tctttgattc
    13741 ttgcagtact ttttaggaga tagatgccct atgaaaccca taaatataaa cttagattta
    13801 ccaattaatc cgtattctat acatattgag aagtcctggg ggggccttga aggggcgcta
    13861 aaacccttta tcgggcacaa gaccatgcta ataaccgatg agaacgtaaa taatctgttt
    13921 gcccgcgata tagcctcgat tcttagtttc atgggatgcc aggttaatgt ggctgttgtc
    13981 aaacccggcg aggcaagtaa gagcctagaa atcgcagaaa cgctgtatac ccgggccttg
    14041 gaggcgaacc ttgacagatc gtcttctatt atcgccttag gtgggggcgt ggtgggcgat
    14101 cttgcaggtt ttgttgcggc aacctatatg aggggtataa actatgtaca gatcccgacc
    14161 accctgctgg cccaagtaga tagcagcgta ggcggaaagg tggcagtaaa ccatccctta
    14221 gcaaagaata tcataggtaa cttttatcaa ccaaagtctg tctatatcaa catagacacc
    14281 ctgaagaccc tacccgagag acaattttcc acgggtatgg ctgaattgat aaagtacgga
    14341 tttatatggg ataacggatt tttaaaatgg ctccaatcca atatacacga tataatgaac
    14401 aggcaggaag aggggcttgc ccgtgctgtc ggatgctcct gcgagataaa agcccatata
    14461 gtaagccggg atgaaaggga aaagggcctt agggcaatcc tcaactttgg gcataccgtc
    14521 ggccatggtc tggaatcatt aacaaactat gacctatata cccacggtga ggctgtctcc
    14581 ttgggtatgg tatacgaatc ctcaatagcc tgtcgaatgg gcctggtgga taaatcaatg
    14641 gtcgatcttc tcattcggct cctcgacaaa gctaacctcc caacggaaat tcatggtgtt
    14701 gataccgagg cgcttataaa agccatggcc catgacaaga agaaccaagg tgattccatc
    14761 gtttttgtac tccccatagg aattggcaaa gtggatgttt tcaataatat tgacagcgga
    14821 ttaatccgcg aggtatttaa gatatcgcct aaggattttt tggctgaacc ttggtgagta
    14881 tagcccttat aacagagtgt aattcttttg tacagcacgt ttggcagtgt gttttttaaa
    14941 aattctccaa atgaattctg tataaattcg tctgttgtgt tggttacaga taatcatgct
    15001 ggtaatggct atttaacgcc aagtgccaat ggagtcatcc gcttttcaat agcccattac
    15061 gcagatgttt tacgatatgc atcttacatt acgtgtcaga tatcgagaaa cattttcctt
    15121 ggcttatatg ttgcgaaaac tacattgatc ttacattgat ctattgatag gcctttttcg
    15181 taacatatat agataacgaa gcagcataac gcattaaatt aggaggaact catatggtaa
    15241 aacaaagaaa tgccatcaat aaccttaacc cttatattcc cggaaagccc ataagcgagg
    15301 tccaaaggga actggggctc gacagagtaa ttaagttggc gtccaatgaa aatcccctgg
    15361 gaaattcccc tgccgcttcc aaagcggtaa aggattgggc ccagaatatg gctatctatc
    15421 ccgatgggaa tagtgcagat ctaaaagcag ctctatctga aaagttggga gtaggcccgg
    15481 aacagcttct gtttgggtca ggaaccgatc aaattctgga gataatcgca caaacctata
    15541 taaatccggg agataacgcc atcatggggg atccgtcctt tcccagatat gaaaccgtcg
    15601 taagggtgat ggatggggag atcataaaaa tacccttgac cggggaacac cgattggatc
    15661 tggagggatt cttaagtaaa gtagattcaa ggaccaagat aatctggctg tgcaacccca
    15721 ataatcctac agggactatg atcaccgcca aagagcaaag ggattttatt gaaaggatac
    15781 ccggcgatgt gctggtagtg ctcgatgagg cctattacga gtacgcattt ggcaacgatt
    15841 atcccgatag tatagggctc ctggatgagt tcaaaaactt gttgatacta aggaccttct
    15901 caaaggccta cggtctcgcc ggtctgagag tcggatatgc aatatccaac agtgacacca
    15961 ttggatacct taaccgggtc aggggtccct ttaatactaa tgcggcggcc caaacagctg
    16021 ctgtagctgc cctcggggat aaggatttca ttgacaagag cattgcgaat aatgtcaggg
    16081 gtaaagagtt cctatactcc tcctttgaag ggatgggcct ggattacatc cccacctatg
    16141 ctaacttttt gatggtgaga gttaacctgc caagcgccca tgtttttggg gaactcctga
    16201 aaaagggtgt cattataagg tccggtgata tcttcggtat ggatgattgg ataagggtca
    16261 cagtcggtac cgaggaggag aacaaccttt tcataaaagc cctaggggag gttctagctg
    16321 gtaagtaaag cccgtcagtg agaccgcccg aaataatgtg ggaaatataa ggattttatg
    16381 gggccgctta aaacaatgta gggaatacaa agggtgaatg gggtccactc aaggcagtgc
    16441 agggaataca aaggcttatt aggcctactc agtgcaatga agggagcacc aagggtgaat
    16501 gggtccataa gcgttgcgaa gactaaggtt catagaaact aacggatcca tccaagataa
    16561 tgtagggaat atggaagtat ggtaatacgg tagactatat atgttgggtg cggcgtctga
    16621 ataattattc aggtatctgc cgcggaacat atagaagcaa tgtaaaataa ttttagaagc
    16681 gaggaatggt agtatggtaa tcgtaatgaa ggctaacgca acgaatgaac agatagatgg
    16741 cgttgaaggt tttttaacaa acctcaactt aggagtgcat atctctaccg gttcggaaag
    16801 aaccatcatc ggggtaatag gggataaacg aatcctgata gatacctccc ttgagataat
    16861 gccgggggtg gacaggctgg ttcctattat ggacccctac aaattggttg gcaagacctt
    16921 tagacccgaa tctactgtcg taaatattaa gggagtcgag ataggcggca cctccttgat
    16981 tatgatggca ggcccctgcg ccgtggaaag tgaagaacag ctcatggaaa ccgctacttt
    17041 cttaaagaaa catggggttc aattcatgcg cggtggtgct tacaagcctc ggacctcccc
    17101 ctattccttt cagggtttgg gagaagaagg tcttaaactg ctggcccggg tccgagagga
    17161 gacgggatta ttgatcatct cggaagtggt aagcaatgac tccttggaaa aggcgcttca
    17221 gtacgtagat gtactccaga ttggagccag gaatatgcaa aattttgatc tgttgcagca
    17281 tgtgggaaaa tccaagatgc cggtggtcct caagaggggt ctgtcctcca gtattgaaga
    17341 gtggttaaat gcggcggagt acatcctaag tgaaggcaat tatcaggtga tactctgcga
    17401 acggggtatc agaaccttcg agaccgctac ccgaaacacc cttgacctca gcagtgtccc
    17461 ccttataaag gagatcagtc atcttcccat aatagtggat cccagccatg gtacaggcaa
    17521 gtggagtctg gttactccaa tggcccgtgc cggcatcgcc gccggagccg atgggttgat
    17581 cgttgaggtg catccaaacc ctcaactagc cctctctgac ggtaatcaat cccttaactt
    17641 tgaaagtttt tcggcgttga tggacgagat ccgcaagatt gcccccatct taaagcggga
    17701 gctcccgcaa tgaccttcca taggatatgt atcgttggtt tgggacttat tggggggtca
    17761 ttggcgaagg ctatcaagag gagcaatccc caatccttta tcacagctgt ggattccgac
    17821 caaaaaagcc tggatgatgc attccttgat aaagtagttg ataaggtgac cgctgattta
    17881 tatgaggctg ttgaaggtgc ggaattgatc tttatctgta ctcccgttca taccatatcc
    17941 ccccaacttg aaaggattgc tccagtattg gataaggata caataataac cgatgtggga
    18001 agtaccaaac aagaaatcat gaaagctgcc gggaaaatcg aggctataaa tcaggacggg
    18061 gctgttttta taggcgggca tcccatggca ggtacccagc attcgggcta tgaggcatcc
    18121 cttccccatc tctttgaaaa cgcctactat ctccttaccc cccttgattc tacaccggct
    18181 tgggctgtgg aatcattgaa gaagcttttg ttgtccatag gtgcgttgcc catggttatg
    18241 gccgcaaagg atcatgataa aatcatcggc gccataagcc atctccccca cgttgtagct
    18301 gctgctttgg taaacacagt agcaaagttg gaggaccctt ccctgttata tatgaaactg
    18361 gccgccggcg gatttaggga tataactaga atcgcctctt cgaatcccga gatgtggaag
    18421 agtatatgta tggataataa agaggagctg gtggcacata ttaccggtat aaacaggcag
    18481 ttggatacct ttacccggat gctattgggg gatgaggaaa ccgaagtcga ggattttttt
    18541 gcctctgcaa gggcttaccg cgacaatctg ccggccagac aggcccatag ccttctgtcc
    18601 tactatgatc tttatgtgga tgtagaggac cagcctgggg ttatcggcga tgttgctaca
    18661 attttgggga aacatctaat caatataaag aatttgcgga tcatcaatag ccgagaaggc
    18721 gagcccggat cccttattat ttccctgtcc gattcaacct ccttggaaag ggcacgaaag
    18781 gtccttgggg aaaatggtta tgataccttt accaaatagc cttaaacgga tttcttgccc
    18841 cctatagggt ggcagcggcg caataataac atgtttacac cggggactaa gtgtttttgg
    18901 aatatatcat ttgaaataca tcatgctggg gggaatcgaa gttggacata gtgctttctt
    18961 gtggaaatcg gttaaggggc acaattcata ttcccgggga taaatccata tcccatcggg
    19021 gtattatctt tggcgccctg gcccggggta ctacacgact tgggaacttt cttatggctg
    19081 atgactgcat aaacacgatc aatatattca gacagatggg catagggata gatataaaag
    19141 atcatagcac tgtcatagta cagggaaaag gtctgtacgg actttctaag cccgataacc
    19201 tgttagatgc gggcaattca gggaccacgg ccaggcttct catgggtctt ctggcaggac
    19261 agaattttga ttccacccta accggggacg attctttaag gagcagaccc atggataggg
    19321 taataattcc cctgaggcaa atgggcgcac agattgaggg gatcaaaggc agtaattttc
    19381 ttcccatcac cgtcagcggt aagggagcca ccctaaaggc tatggattat accatgcctg
    19441 tggccagcgc acaagtcaaa tcctccataa ttcttgcatc cctctatgcc cataagagct
    19501 cgtatattca ccagcctgca atttcccgga accatacgga aataatgctt gggacctttg
    19561 gggggagaat ccatacccaa ggaaagaccg tcactacctt tcctggggcg atgctttatg
    19621 ggcaggattt ggttataccc ggggacatct cttcggccgc ctattttatt acggccgccc
    19681 ttttagtgcc cgattcggag atagtcttaa aaaacgtcgg gataaatcct acccgctccg
    19741 gtatactcga tgtgtaccgg gctatgggtg catcaataga cgtggaggaa attcataccg
    19801 gaggacaaga accctcagcg aatattatcg ttaaaagctc ccaattaaaa ggcaccgtaa
    19861 tcgaaggttc gcttatcccg aggttgattg acgagctgcc tataatagcc ttggcggcta
    19921 ctcaagccga gggaaccacc gttataaagg atgcggcgga attaagggtc aaggaatccg
    19981 acagaatcga ttcgaccgtt gccattctga aggaatttgg cgccgatgca gaagctaccc
    20041 cggatggaat gatcattcgc gggcctgcac ccctatctgg gaccgggctc aggccgggct
    20101 cggatcatag ggcggttatg atggcggcaa tagccggact aatagcccaa ggttccacaa
    20161 ccatccggga tgcaaattgg gttaatatat cctacccggg cttctttgcc cagatagaag
    20221 cgctccgatg ctagacatcg aggcgctttg ttcatgtacg atatgctttt tggcattcca
    20281 tatatctgac cgatcattac tccagctctt tgcgatactt ttccatggag atcttaaaca
    20341 gttccccggg ggttggcggc gtgtaggtga tggcatttgc ccccgcttca acagtcctaa
    20401 tgatagtttc atcattgggt cctcccgttg cgatgatagg cacttcggga aattcgtccc
    20461 ttatcctttt gactatgtct atggtcttac cggcccctga aacgtttaga atactagccc
    20521 ccgcctctag gcgagattga atgtccgttt tctcggagat gacggttact actatgggta
    20581 tatcgatggt ggcacgcacc gccattatgg tttcgttgtc ggtaggagca tttaccacta
    20641 cgcctatggc cccctcaaac tctgcgtcca gggcgaggtt tactaccctc ttccctgtag
    20701 tcacccctcc ccctacaccg caaaacaccg gaacatcggc ggccatggtt atggctttgg
    20761 ttatggccgg ctggggcgta aatggatata cggctattat agcatccgca ttgatattct
    20821 ttatcacggc tacatcggta gtaaatgcca gtgacttgat tctcttaccg aatattttta
    20881 tgcccgttgc cttccggata ctgtccggca cctctatcat atgtcttctt aaggtccccc
    20941 ttatctcagg tacaaatctt tccatcttgt ccctccctca agctttatta taacgataat
    21001 ttaacccatt acagggtcta aagtgcccat ggtgattgct taacattcgc cctagcgcca
    21061 atccatatag agcaagcctt atttagcata aaaaatcggt cccattcccc ttaccctatt
    21121 ttaacatact accaaaggct ttagaaaccc cctctatgga taaaaagacc cccgttatca
    21181 actaacgaag gccaagcagt tgtaatatat ttgtgcaacc atcagggtac tttgcgattg
    21241 ttgcgcatcc gtaccaaata tccaccataa tacgaaggct ataacgatga cagctatgac
    21301 ggcaaccaac cacttcaacg tattcttacg gaaataaaaa atttgcatcc acacaccccc
    21361 acggcacaat tttcatacac tggcattata taaatatgcc cgaaggtgtg ttattattac
    21421 tgcctaggag gtcaatcttt tcagcagcgc taaggatgca ggtccttctt tatttatgtc
    21481 ctttgtaccg gcttcaatgc tgactctgcc cccatagcca atatcaccta agttggtaaa
    21541 aaaatcgttg tatatatcct cggattcatc aaggggaaag gtcctggttt gcccctgggc
    21601 tatatgtgtg tgaaccaaaa tatcggctgt ggtttcccca ataaccccca tgggctcgtc
    21661 ttccatcctc atatgataaa agtccgccaa caattttacg ttggggtgcc gaacctcatc
    21721 aacaaactta attccctgag ccagggagtt tataatattt gtttccttct tatttagtgg
    21781 ttccaccgct accgtaatgt tatatggaag ggccaattca cctatcaacc cggcaacctc
    21841 aacaagaccc ttccatccgt cctcaaaaac ccaatccgaa ggaaccctac gggccccgcc
    21901 gctgccgaag acgactattt gggctccgag tcccgctatt ctttcaaagg catcctttag
    21961 ataatcgcgt attttacaca gatcagcttc aggaccgata agtcttatat cacccggaaa
    22021 cagcacatta aatgtctcgc atccgatccc ggcatctttt attttcttaa gcgcctcctt
    22081 gtattctatg ctactcatct ggacgatgcc cgtaaccgtc ggttcaatat agtcgaaccc
    22141 aagcccttca accgctttga tttcttcaat aggtctgcat attcctattc tcatactaat
    22201 aggtcccttc tacgtccaga gccttatccc ctgcggttcc cttttgaaat gtggagccct
    22261 ggatcttttc cttcagtttt tggtagaaga gatcttcatc caagggaatg cttacccagc
    22321 cgtcagtcca tgaagatagg tgcatagcat tggatatggt caaaccgtta ataccctcga
    22381 tgcctgggga taaaagcgga gtccccttta caatagcgtt tacccagtcc tgggtaattc
    22441 cctgatgggc tgtctcttcc cccgaaatgg gtatctcgca tttccagcac tccggttcac
    22501 caaacccgcc tttgtattct ctgttgaatt gcctttctga aaccctcagc ctccagaacg
    22561 ttagcttacc gtcttccgct atcaatttac cccgatcccc ggtaatttca agcctattgg
    22621 ttccgggagc atcccctgtg gatgtgatga aaacccctgt ggcgccgttg ggatattcaa
    22681 cgtatgccgt cacatcgtcg tctacttcta tatcgtggta tttaccgtag cttatgaagg
    22741 cctgaacctt ctccggcatt ccgcatatcc attgccagag atccaaatta tggggacatt
    22801 ggttaagcaa aaccccaccg ccttcacctg cccaagtagc tctccagccc ccggagtcat
    22861 aatagctctg ggccctatac caattggtaa ttatccagtt ggttctcttt agttcgccca
    22921 gttccccgga ttctaccaga tccttcatct tttgatacag ggggtttgtg cgctggttgt
    22981 acataatgct aaatatccta tcgctcttgt ttgccgcatc gttcatctca cggacctgtt
    23041 tggtgtatac ccccgccggt ttctccagca aaacgtgtag gttgttattg aatgcctcta
    23101 ttactaggga cggatgatcg taatgcggag tcgcaataat aaccccatcg actaaccccg
    23161 atgaatacat gctgtccgag ttttcgaaca actctactcc gtttccgata ttatccctag
    23221 cccactccag ccttttggga tttatatcgc atagcgccgt taattctgcg tttttaacct
    23281 tgttttgaga caggtacatt gcatgggcgc tgcccatgtt cccgatacct actataccta
    23341 ttcttacctt gtccatatta gtcgtccctt cccttcatcc acatttttaa gaaagcataa
    23401 cgtttgcaat atattatttt gaccggtttt gatcataagt atcgtttata atcgctatcc
    23461 agatcaaagg gttccatagt taacgccgga tgtccgggtt aactagcagc acaacggatc
    23521 aattacgaat ccaggcccag tttctgtaga ttcttaagac taatctccag actctcgaaa
    23581 ggatccctct ggcagatatc ctgttccacc gctgcccatt ctatcttgtt ctttatgctg
    23641 gcatctatga tgtccggcca attaagattg ccttccccga cctctgccat tatctgctta
    23701 acatccttgc ttatggccat atccttgtaa tggattacct gcatcctgtt tccgacctta
    23761 ttgatccaat caactggatt gcatccgccg gcctgaaccc aataggtatc gatttcaaac
    23821 ccgaaggctg cggggtcgga ttcgtttagc aggatatcca tgcccaatac cccgtcaaac
    23881 ttaataaatt caaaatgatg gttatggtag ataaactgta atccattatc agccagtttc
    23941 tttccgatct ccgatgcctc cttggcaaat gcccgatatc cttccttact ggattggtat
    24001 tcgccgggca tacttcctac cccaacatat ttgcagttcc atagtctatg ctggtttata
    24061 acatcgtcta agttttcctt catgctgtca aagccgatat gagtagcaca aatggttaag
    24121 ccgtggcggt ccataatatc ctttacctgc tgatgctcca tgggccctat ccctgaaacc
    24181 tgcactgcgt tgtagcccat gtccttaatc ctttccatgc tcttgtccag ctcgcccggc
    24241 gttttagtaa aatctcgtat agtgtagagc tgcgctgcta ttttaggttt ttgcatagta
    24301 taacactcct tctttattac atatcatcta tcattactct gtttcccgtt gaagatgatt
    24361 tgtagattcc gtccaatatc tgcagggtcc acatgccctc ctccacggga ataatggctt
    24421 tccctgcacc gttcataata tttttgtaga aatgccttat aagggtatca tgactcttcc
    24481 cccagtagga tttatagggg gatgtttccg agttctcgtc gatgagtttt tccgatttgc
    24541 catcctccac ccttataagc tcgttatcgt atatcctcag cattccttta tccatatgta
    24601 tttcaacctc tacggccgaa ttatcggtat agcagttagt ggcataaaag atacccctgg
    24661 caccgttttt aaagtacaag gtggcctccg cggtatcctc tacctctatt acatccttta
    24721 aggttctggt ggatacattt ccttgtatgg attccacctc acccaccagc cagcgcatga
    24781 ggtccaatgt atgtatagat tggtttatga gcactccccc gccttcggtt tggaagctgc
    24841 ctctccagcc gctttccata tagtaatccg catcccgaaa ccaggtgacc aatcctttta
    24901 ctcccctaac tccacccatc tccccgctcc taatgatctc ttttgcttta atagaggtgg
    24961 ggttaaacct gttttgaaag ttaatggtta tgtatttgtt gttatccctg gcggccttcg
    25021 ccatcgcctt ggcctgctct aagtttatgg cagcgggttt ttcaaccagg acatgcttcc
    25081 ccgcgtttac tgcatcaatg gccatttggc tatgtaagta atggggcgta catatatgta
    25141 ctatattgac ccccggatct ttcagggcct cccggtagtc ggtgcaccag cccccgccat
    25201 attccttagc cgaagccttc gccctatctt ctttaagatc cacaaaccat agaattttgc
    25261 ccataccgtt attcataata gcatctgcat gaaccctgtg gatcgctcca catcctataa
    25321 tagctacccc aaaaccttcc atatccattc ctctttcctc gggatccccg attaatatac
    25381 taatgtctta agcattgcct ccactataat aactcggcat gtctaagaaa aatgaaccgg
    25441 tataccaggt taagttaacc tataataatt atagcatcga ccgtacatta gtcaatgtac
    25501 aaaatctctg cccccgagcc atgccctaag ggttttcgca tcctctaaat actgatctac
    25561 tctgtcgtct ttcacaaatt cgattaatgc atagtgatcc cccggaatat cttcaagtaa
    25621 ttttatatag tcatgccatt cccgacgtcc gtgatccaaa ggtaaccggt tcccgtatat
    25681 ccgggcccta ttcctgccaa cgtcctccaa ggtgctctgc cagaaacttt tcagctgcag
    25741 tgtagaatgt atttctgtca tccggtgacg aaaagccatg ccccgcattg ggaaacagta
    25801 gataccttac atccaaaccg tgttcttcca tagccctaac aatctgatcg gactccgatt
    25861 gttttaccct ggggtcattg ccgccttggg caataagaac cggtatcttt atttgatcaa
    25921 ccttaaaaag aggtgatctg gattctagaa attccttttc ggtttttaaa ttccccaccc
    25981 tcctataata attatctagg ataggcgtcc aataaggggg tatggatcct aaaagtgtaa
    26041 ctaagttact ggggccgaag agatcgacag cgcaagcaaa tacatcaggt gtaaaggatg
    26101 cgccgacaag ggcagcatat cccccataag aacccccgta tatgcctatc ctttttggat
    26161 cggcataccc tttatctatg gcccagttga ccccgtcgat taaatcgtca tgcatcctgg
    26221 ctccccattc cttgttaccc gcatttaaga agtccttacc ataccccgaa gaccccctaa
    26281 agtttacctg taagactaca taaccgcgat ttgccatcca ttggatctcc ggatcaaatc
    26341 cccatatatc cctggaccag gggccgccat gaacgttgag tatcaagggc agatcctccc
    26401 tttctgatcc aagagggaaa gttatatacc cgtggatagt aagcccatcc ctggatttat
    26461 aggatatcgg ttcagtctta gccaaggtat agtcatttag atccggtaat tcatcgaata
    26521 ggtggttgcc ttcatccttt tcgctatcat atatatagta ggatagtggc tcatcatcac
    26581 tggtaaaggc cagcaaccaa accctatcat cctcgctgcc gcttacaaag gagacatccc
    26641 catcgtttaa cttggagata gtttcgaatg ctttttcggt atcggcgtta aaaaacaccc
    26701 attcctttct ctgctttata aaggaagccc cttggatctc ataggtttcg tcgtcgatta
    26761 atacctcatt tacgtcgtag tcgggatctt ctattagtac cgtattttca ccggtttcaa
    26821 tgtcgaccct tattaacctc cccgcattgg attctcttga atccaggatc aatacgctac
    26881 cgccatcttt tgtaaagcct aagggtgcgc tattattgga tgattcataa tcccaagaaa
    26941 taagggttct ccagctattg tctctgttat cccgcaccaa aatgtctgat cccccatcgg
    27001 gatttgatgc taaagcaccc ctgacatcac ctttgtcatc tgtcatccag ccttgtatgt
    27061 tcccggggtt tctctctagc aattctattg agccactgct tatccctacc ctatagacat
    27121 ggtggttagc ggggtcatcc ttattaaggg cgatcaatac ttcatcgggt cgcttgctgc
    27181 ttgcttctat gaccatagcc tgtatatcat catagggtgt taaatccttt atcttacccg
    27241 tctcaatatt ggatgcgtat aggtggtagt tttcatcccc caaggaatct tgcaggatta
    27301 aaatatgctt gttgtcacct gcccaaaaga acctataatt tccgggggta ctgggctgtt
    27361 ggactactat gtccctgcga tcctgttcca gatctcttac taatattgta tcgtatccat
    27421 cttcatacag gcggtatgca agcctagtac cgtcaggaga tatgctgggc gaggtcctca
    27481 cgggatctgc aaagagaacc tccctgggta tcaggtctgc ttctccttga tcagcctcgc
    27541 ccttttgatc cgttttataa tcttctattt tttctgcgtg gttaccggag tctatttcat
    27601 cgactctaac gggtaatatg ccattttcac cgacgcagga cgacattacc aaagtcaaaa
    27661 caaccagaat taatgctatg gatcgcagcc taaaaaattt catggggcgt ctcctctcgg
    27721 atctatattg cttaaatcat tgctacaacc tctttcgctt atccccctta cgagccttta
    27781 gcagctgggt tgtaaagccc ggggcctcat cttcagggtc cttcgacgtt ccctcatgga
    27841 cgtttttacc agccgttttt ggggatcttc tctccttttc tatccccggc tcagtagccc
    27901 tttgacctct tgccttttga ttttctcccc tgactatcct tcccccatga ccctggtctt
    27961 ttgctgcctt atcccccgcg agtttaaaat gttttcctat tagatccgct atattaagcc
    28021 ttctcatggc tatatctaaa acaaataaaa gtaaggctat ggggagcagc catggccaga
    28081 tccccgtttg ggtccatacc ggctctgggt cctccctgaa aatctcttcc ggattatcta
    28141 ttatccttcc cccagtctgt gcgacgatct cctttaaaaa atctatggaa ccggactgcc
    28201 tgagatcata ttcgggggaa tagttaaccg ccaaacccgc ctctaaagta tttactattt
    28261 taccgccttt actatgccgg gtattcacta tatatacccc gggcattgtt ataccaaaat
    28321 taccctcgta ctttcccggc cgggttactt caaggggcac tatggtttcc ttaccttttg
    28381 gaccaataat tatagcctcc ccatccaagt cttctttcat attgtgatcc agcttcaccg
    28441 atatttgtcc ctgattacca tgcaacgatg tctctatgaa cccttgggaa tcatcaccaa
    28501 agggtagaat cctggatatc actcccagcc agaattcctc cgtttgatcc caactgagcc
    28561 aattctttgt ccatgccccc cggatatcgc tggtccaggc taccaccctt ccaagcccat
    28621 attgccactc ggccaatata gggtccatgg tatcgcttga tagagccatg gtggctgtat
    28681 tctttgaaag cgtagtgata tatccgtcaa ggggcggaaa accctcttta aagccgctta
    28741 taatcgggga atgttcggtt atagtaggga aaaatgtacg attttgtata tagctctggc
    28801 tggccatacg ggtttccttg gtaaatatct tggggatatc ggcgaattcg tcagtaaaat
    28861 aataccgccc acccccgctt ttcgccaatt cttccaatag gaattgatcc gcatcctggc
    28921 ctacggccac ggcagaaagg gttatcccct cttttttcat tttatccaac atcccgtcat
    28981 aatctccggg catggattgg ccgtcggtca tcaatataac atgctttagc tttgtattga
    29041 gtcctcttaa ggattcgtaa gccagggata acccgggata tatattggtc ccgcccatag
    29101 cgggtatgga gagaattttc tcatggatcc catccagatc ctcggggtgc tgagtattta
    29161 ctacccaaag cgcctcgcta tcgaaggcta ccactcctac atagtccatg gttcttaggc
    29221 tttctgtaga tcttatagcc gcttcctttg ccatatccat cttggaaata ccgtattgcc
    29281 cgtcggccat actcccggat ttatctataa ccaacaccag cccaagatcc gggacctcgg
    29341 ccttatggtt caaatcgaga tccaccggca acatctcagc cagttgggta tccatatatc
    29401 cgccaagagc atagctgtta tccccacctg taaataggat tccccgccca aggctttttg
    29461 catagacgtc aaggatattt attttttcct cgccaagatc atcggcggaa acgttacata
    29521 gtatagtccc ctggaacttt cttaactcct ccaattcctt tgggagagaa gatggatcat
    29581 aaatcttgta gttcatcccc gcagattcta aaattcctgc aagctccctt ccatcgccct
    29641 cctgtccttc taccaaggcc aaaattggaa tcccttgcac ctcagtgtat gtgactatcc
    29701 tgttgttttg ctttatacta tctcgcttta catccaattc cgcctcatag gtcttaatcc
    29761 cgccctctag agcggtatcc ttgaagataa atcggttttc accctttaag atctctatcc
    29821 cttgttctcc aaccaggctg cggtttgcat atagcctaat atttccatcg gtgtttatcg
    29881 tactgtctat ggtaatagtt atgtcatagg cctcgccttc gtagagcttc gtcggtacgt
    29941 ctaaagaaga gatctgtact tcagcgccgg gttccgtatc aaaaaagacc ccatctatgc
    30001 ggatcccttg gtgggaaagg cttttagccc tttggatggc ttcgcccatg ttttcgttgc
    30061 cgtcggtcaa aagcagcaca cgcttttggg tatcatcggg catcaaggcg ctggccatct
    30121 gaagtccgga acctatatcc gtataattgg gatccgggat ggtttcaagt ccatggaaga
    30181 tcatatcaaa gcttaggggc tgctctacaa aggtgtcctt tccgaaggta ataatccccg
    30241 tttgatattt agccggctta ttctccatgg atctgcgcat aaaatcggcc atggatcccc
    30301 ttacttccct cgtgctgttc gatagatcca ccgcaaagat aagagttgtt ttgtccgagt
    30361 atttttttat actgaatccc gccaaggata aaatcaataa caaaactatt atgaacctta
    30421 acacaagaga atatttttcc cccgtccttt ttatcctgct atttttgacg tagccccaaa
    30481 gaataaaaac gcatgatggg acagccagca gcaagatcca cggatttcca aagctaatac
    30541 ccacgctgat acacccacca ttctgcaagg atcaaaatca ataccgccca gataaaataa
    30601 tgccaaacgc ttttacccgt agaaagcctt aatctgtcat ccgtctgtat ttttgtttca
    30661 gtgctcggct ggtcaatgag ccttaaatcc gattctttta aagtcggtat gtgcacagta
    30721 aaatatcctt ctatatcctt ttcgtctatt ctttgtctaa ccttgtacac acccacttct
    30781 tgggtttcgc taaaaggcgg gataggaaac ggcgggccta catccatggt taatccgtag
    30841 gggttctcta ccgttacctt ttgggcatgg ggaaatgggt tgatctccac ctcgtccccc
    30901 gcgaatcttt cggcatcgta gtcaactatc tgggggagca tccaccccaa tagattctgc
    30961 ataaggattg gaaaatcaga ctttaggggc agatcacttt tatgaatctc aaagggaaag
    31021 attgcaatcc tttggttctc caattgccca accatcatta tagggcgctg cttttcatcc
    31081 tccaacacca tttcggccca ggggggcttt tctaatatcc tggcctcggc tatgtgaaaa
    31141 tcctcagggg taatatagtt taccaaatcc ctgtaagagg ttctgctatt tagcctgata
    31201 gcccccggat taaatatatc acctatattt aaaatatcac cggtacccac cgggttaaag
    31261 agtattacgc tgccatcttc aggtaattcc ggcggcgtgt acccatcata gacatatagg
    31321 tcgtatccgc caatatcccg ggccccatca agggtggtct tatcaagttc caggtcccct
    31381 ctaagaccta tggccttctc cagaaatgta tttctggtag taaccagcaa aaccctattg
    31441 acagaaggcg gacttagtat agcccaggcc ctgttatccg ccataagatc atcttcatcc
    31501 aagatatgaa tctctatcct ctgggctttt gtgggtatat tatcccaata gatgttttta
    31561 gtctcaccct cagataagct gacctcttta acatctatca atctgccgtc tgccaagcat
    31621 tccactgaaa ccatagaatc ggatccatag tttgtaatgg cggatagaat tataggcgta
    31681 tttgaatcat agctgtaggt tacggattgt actgctctgt tttgaccgtt atcgttatag
    31741 attatggtat tgtaagggtt atcctcatta tcccctgcct tatgatattg atcggaatag
    31801 agatatatgc cagaatttgg ttcttccttg gcgagggccc tgacgaactc aaaggcttcc
    31861 accatgttgg cgaatccgtt ggtctctttg atctggccca gttttgcctt aagggtagcc
    31921 ttatcggaga cttgatttgc gagaattctt gcccgatccg atgcctcaac tagggtcatg
    31981 cgctggtggg gtaacattcc gtctataagt ttttctatgt cacttttggc cctttgaaac
    32041 cggctgggct ttatatctgt ggcctgcata ctggcggatg aatcaaggat tatgatataa
    32101 tcgtcttttt cttctctgct atataaaaag ggctgggata tacccaatga aaaaaggacc
    32161 aaggccataa gctgaagaat caaaaggata tttgccctga gtctttgcca tggcacactg
    32221 gcctgaagat tttgcatggt cctctcccaa agaaatgtgc tggataccat catctcttgg
    32281 ttctgctgct ttaataggta taatattatt atgccgggga tggatatgaa aaataaaaat
    32341 ccccaaggat ttaaaaactt cacgaactga ttacccccat cttgaccaag ttatcaaaaa
    32401 caatgccctc tataccggta ccgctgttga taggaacata gtgcacaccc ctattataac
    32461 aaaattctct gacatcccgt aaaaaaccgt caagtgtatc cctataggcc ctcataacct
    32521 gctgcgtaac attaatatcc ctatagtttt ggtcttcctt gtcaactagc cttatattgc
    32581 cgtcccatgg cgggtctaat tcttcaggtg agaggatatg gattaagctg atctcctgct
    32641 tttgatattg cagatacttt atcccctctt cataaccgtc ttgcgaaaac agatccgata
    32701 ggattatggt cgtgccccca cggctgccta tggattgaca catctttata gcagtattta
    32761 aggacgttct gccgccaaaa gggatctcct caataaattt aagtgctttc caaaaacctt
    32821 gttttcccgt gaagtatggg agccggtctt ctaccttgtc attgatacac aataagccca
    32881 cccgatccaa ggtattaagg cccagataac aaaatattcc cgccagctgc ttggccaaaa
    32941 accccttgtt tggatcccca aagtccattg atccgctaca gtctaagatg atagtgatct
    33001 gagattgctg ttcttccata aagagcttga taaaaagccg ttcgaatctg gcataggcat
    33061 tccagtcaat ccttctaaaa tcatcgccgg gagtatactc cttaaaatca gaaaactcta
    33121 cggagtctcc cttgaggttg gattttcttt gcccgcctgt actgccctta accgccattt
    33181 tacttgccaa ggacaatcta ttgagtttgt ttaaaaagtc ttgatcaaag ggggttttct
    33241 tcatttttgt tcatcccctt tctattcgcc aagttccccg ataatttcct ccagtacatt
    33301 atccggattt ctaccctctg ctatgccttc aaaatttaag attatcctat gcctcaaggc
    33361 aggatacgcc acatacctaa tgtcatcaaa ggatacatta tacctaccct ccattagggc
    33421 tcttattcgg gctgcgctta taatggcctg agcaccccta gggcttgaac catagcgcac
    33481 atatttttgt gcggcggtcg atgcgttttc gccctcgggt tgggtagcta acaccagcct
    33541 cagggcatag ttttgtaccc cttttgctat agggacctgt tgggctatgc ttctaagctg
    33601 taatatagtc tcatcttggg ctaccttatc cgcctttttc tcttcaccca tggtggtgag
    33661 atttactata ccgtctaatt catctagtga aggaaacttg accatcagct taaagaaaaa
    33721 cctgtccagc tgggcttccg ggaggggata ggtgccctct tgctctaatg ggttttgggt
    33781 agccaacaca aaatacggtt gtggaagttt tctggtatcc cggcctaccg ttaccgtatg
    33841 ctcctgcatg gcctccaata atgcgctctg ggtcttgggg gtagcacggt ttatctcatc
    33901 ggccaatacg atatttccaa atatgggacc cggctgaaat tcaaatctac tgttaccgtc
    33961 tctatccctg gctataatat tggttcccac cacatcagcg ggcatcaaat ccggcgtaaa
    34021 ttgtatccgg ctaaattcca agtccaatac gttacctata gttctgacga gacgggtttt
    34081 accaaggccg ggcactcctt cgaggagggc atttccgccc gctattatgg tcataagggt
    34141 ttgcctaata atctcttttt ggcctatcat ctcccggccg atttccttct ctatcgcctc
    34201 aacctgccgg gcaaaggact ttattgtatc ctgagttatt tccattgtat tctcccccca
    34261 aattatgttt acgaagaccc tgatatcaat ctattgcgct agttctttac caagataatg
    34321 agctattgaa aagcggatga cttcattgat tgggcttaga aaacttggct tatggaatgc
    34381 agaaaagccg tagcttaagg caggacgccg gattggaagc gataggcttt tcaagcggat
    34441 attattccag ggcggtaaaa taggacttta tccattctct catattgggc ggtatactca
    34501 tccgttcaat atttcgcatc gcctgattct tgtattcacc aaatacctca tcgtaaggta
    34561 caaagccgtc aaagtccccc aatccttggc ctgtttctat ctgttgcccg gagccctgtt
    34621 catttatatt gcctttaacc tgacttacct ctccgccgtc ccccagcctc tttggatcca
    34681 atattcgctc atattccgga tgggcagtgc ctatacccgc cccctggcca cccccggctg
    34741 aattgctatc tgaaccgccc tgaccacctt gaccctgtcc gctttgatta ccctgtcctt
    34801 gctgtccgct ttggctgccc tgtccttgct gtccgctttg gctgccctgg ccttgctgtc
    34861 cgccttggct tccttgtccc tgacccggtt cttctatgtt gccctgacta ttcccctggt
    34921 ctgcatttga attaccacta tcgccttctt gattatccgg tccatttgat tgagcgagct
    34981 gggatttatc gatatcaaca ctgcccgcct ttgatatctt atttttcata tcctgcaagg
    35041 catacatgag gtcctgtgtt tcgcttaatg ggccccctgc caaatccata agtaagtctt
    35101 ccaaaacccc caattcctcg gccaaatcca tagtatcttt atccaggttt tttcctatat
    35161 ccccagctaa ttccataagc cggtcttgga ttttaccctt tccgagttta tcggccatct
    35221 gctccagcgc atctttaacg gcatccattg cgtctttatc cataccgcca tttttagcca
    35281 attcctgaag gcgccccatt tcttccttta taccctcggt gtccatctcc tttatttgct
    35341 cgcctaagga ttttaaggcc tcctgcctcg acagcccctc tcccagatcc gccatctcct
    35401 gccttttggt ttccttggcc agctctataa tctcttcctg agttttagat atctctttta
    35461 aggcctcgcg gtggtcccta gtctttttta gtttatcggt tagccgagcc agaagctttt
    35521 taatctcttc ccgttcctta ggggttaatc cggcttccat ggccaattct ttgttgcttt
    35581 catcctctaa tcttttaagc tgctcttcta tgactttttc ggcgtgtaac cttttcttta
    35641 ttatcccatc ctggggattg ggaataaaaa aaccagacat caataacatt agaaggccca
    35701 tcgctataag gctgctcttc tttgatgtag taatgtcgat gtttttagga tcgaaggcct
    35761 ttagcctgtt taaggtatca tgcctttggg cctttgcgaa ttggtcatcc ctatggctta
    35821 attcataggc tgtagtaatt cgttcctcaa accccaactt gtctgctttt ttagtcatct
    35881 tagaaataga aggtcttgta acccatgaga cgagaaatgt tatggccata acaatcccac
    35941 ctgtataggc catgatactc ttaatatgaa taatggggat aaggaaggac acccccatcc
    36001 acaggatacc ttgcccaatg gcagctattg atccccagat aacaccgtga attaaatgct
    36061 gggccgtaag ccgccttcta ataggcccaa aggccctatg gattttagaa tcaacacctc
    36121 tagacattgg tactcttccc cccttttgag aacatgagtt actttgatat attcgccaaa
    36181 tgctttttcc taaaacccct gcgaaccggt tttatcttcc atgcgctaat agtcaacaag
    36241 gtccctgccg cagacagcat gataacagca tttctaatcc ataggctcca ttggctccat
    36301 gaccatccat ttatcatgcc ccctgtattg gggtttgacg tgccataata ccccatcccg
    36361 aacatcattg agagtacgcc tgccaccccc aggttccccc cgcttcccga ttgcctggcc
    36421 agtatgtccg ccagtcccgt tacaggattt aaatagaata cgaaaggggt ataagaatct
    36481 aaggtcattc cgctgacgct gctactatta tagtagtagg attggagcat agtcgcccca
    36541 attatcgcag ttaatatgcc caaaacaaag atagtcagat atgatattac ggtagaagta
    36601 acggtctttt taaacagtac agagcaaaac atgcctatgc tcccaatggc aaaggcggta
    36661 atcatataga aagcgaacaa cacgacaata tccccgggag ttaccccgcc aaaaagcagc
    36721 gctaaactga atatgggaat cgaagatatt ataaggagaa atataaagct catagaggag
    36781 attagctttc ccatgacgat gccggtgggc gacatcttgg tacataacaa cagatctaaa
    36841 gtctgttttt cacgttcgcc gcttatactt cccgccgtct gggcgggagt aatcagcact
    36901 ataagggcga actggagtat cgccaaggta gtatatatct gaagtccgat agaattatta
    36961 acatcaaagg tatttccgta gccataggta ttatcaaagg tcaagttgaa aaagagatag
    37021 ctgatcaaca gcattattcc aagataaacc gtcaccgccg cggcagtccg ccatcccctc
    37081 atctttattt ttaactcact taccataacg ggattataaa gccatttctt cacccttggt
    37141 cacctccata aatatctgtt ccaaattacc gtctgccttg ccaaaggaaa gaataggtag
    37201 ctcttgattg actaaaattc tcaacagctt ccagagatct tgatcctccc cgctgtaggt
    37261 tacttctagg gagtttcctt cttgaattat ttcctcgact aagggctgct ccttgagtag
    37321 gttaatcgcc tcttccaatc tatccataaa cttcaaacgt attatagtcc ttcccgaaat
    37381 tcttctcatg atatcttcta cactgccgtc tataaccatt ttcccgtttt ctataattcc
    37441 tatggtgtcg cacatttctg ccagttccga aaggatgtgg gagcttataa gtatggtctt
    37501 gttcattccc tttagctcct ttaatatccc ctttatctca attctggccc tagggtcaag
    37561 gcctgatgcc ggttcatcca acaccaggag ttcgggattg tggattaggc tcctagccag
    37621 gcataacctc tgcttcattc cacgagagag ggtatccaca tagctatctt ttttatgacc
    37681 taagtctact aattccaaca gctcgccggc tgccttctgc ctttccttat agggcatccc
    37741 ataactactt ccataaaagt ccatgtattc atggacttta aggttatcat aaaccccaaa
    37801 gaagtccggc atatagccga tcctttccct tacctttagg ggttctctta ctacatccat
    37861 accctctact tcaacagtcc cggaggtggg ctgcatcaag gttgctatta tctttatggt
    37921 agtggtcttt ccggcaccgt tagggcctac aaacccaaat atgctgccgg attcaatctt
    37981 taagttgacc tggtctaccg ccgtaaattt accatatctt tttaccaaat ccttaataac
    38041 cagcattaac gcttcctccc ctcaatgatt agggtagggg cttcaaagga gatgtactcc
    38101 ctcggtcttc cctctatttt aatgagcatt ttcccttcgt cgtctataaa tctggatatg
    38161 gagtcggggc ttataataat agcccccctg gccttatggt aggtaatgct atcgtcttca
    38221 tccatataat ccccctcccc tgcgtcgagg ggagatagac cgatgggatc gccatagggt
    38281 cttgcaacca cctctatctt ggttaattcc aagtccatat agggagtcat atcaaattcg
    38341 aagactcctt taccccggtc caaataaaaa atcgaatcgt cggaatgaat catatcggat
    38401 aattcctttt taagaatccc ctttacataa cccggaggta tgccgacttg gccatctctt
    38461 tcatagacga tatcaaaacc ggacaaaacc acaccggtat tttgtggggt aaccggtcct
    38521 gaaccaacta ccgtaatctt ttcttccaaa tcataatcgc aaaatcccaa caggttgata
    38581 aagtcgttat gcaataaatc gttgccgtca tatttcatta tgagcccctc gacaaagttt
    38641 cgctttgccc acctactccg ttcctcatcg ctgggctgcc tgcttcccga attggggtat
    38701 ggaaataggc tgtcgatcat catatacagg tcgttatgcc cgtaaccaga ttgcgtgctt
    38761 gggctggaaa ctgatagatt gacttccacc tcaacgccgg gaattaggtt tcctatcttt
    38821 tcaaaatctc ccgctaaggt atatatagcc gaatcctcta taaggaagga agtgttatta
    38881 ataattgtac ccttaacctt gccatcctta taatacagat ccccttttat atcgcccaag
    38941 ttctctttga agcccctgta cataagggta cgcatagtcc atacattgga atcataaaga
    39001 gtaatggagg agggattacc ctgggaaacc ttggctttta taattccccc atggctgctc
    39061 ggaaagctag aaccgtatgc atagcccata tcaccttccc catagggtgt catgaggaca
    39121 tccccatcta tactgatgtt ataatcccct cttttaggta tgaaaactcc tacataggta
    39181 tctattgtgg cggtattctc ccttgcgctg atggtggaaa tagtgtttat tatgacctcg
    39241 ctaccctttt gtgaatagcc aatcccgtat ataccaatcg aaaacaatat cacaaaaccc
    39301 gggatggtaa accacatcca ttccctttta tctaattttt ttaaaatgat atagttaata
    39361 ggtcctacca caaggagata tcccaaaaga ataattaaga gattggtaaa ggggggtaga
    39421 tccagggctt taatggaact caacgcctcc ctgataccgt agttataccc atatccataa
    39481 ggttgataac cgctagacga caaatcagga cccttgagcg tcgctataac gctaggtttt
    39541 aggttattcg tgagcaccct ctcccaaaat gttttattgc cttcccagct tgtaaaaggc
    39601 tctaatccta aatcaaatcc cgcaataaag atattgcctc tacccgagtt caatccccat
    39661 attattgcct gtccaccatc ctccataagt gtcttgctgt cttggagaga aagccctgct
    39721 aaatccaagc tcatattccc tattccctca ataggttctg atttaatagt gttgccaatc
    39781 tggccatcga caatagtcgg cttaaggcct gataaggttt tagaagcatt agaaccggtc
    39841 ccgataatga ggataccccc attatttacc cactggccca gcacttgctg ctgctcctgt
    39901 gtgaataccg cggtatcggc gttatttatt actatcatag caaagttttc catgacttca
    39961 gggcgatggg gtatctcgtc tttgtttatt agaaccgttt catggtttgc aaatacgctg
    40021 tcgccaccta aagtgcgcca ccagtatcca agtgccggct tatcatcggt tatcacgccc
    40081 ataaggtatt tatttaaggt cataggtttt aaagacttta attccaagga ttttaaggtt
    40141 ttattctttt gctttagatc aattttaaac tttctctggg ttgtattgat ctgtgcatac
    40201 agcataaaac gttttttcga attcttagct attaccgcag ctcttgaata ggttatggcc
    40261 gtaccttgat cctgattgac taatacctcc agatccccat ggatatcctg acctaaattt
    40321 tctatttcaa taacgatggg ggtccatgaa cctacccggt aatgattatc aaatccgatt
    40381 ttagcctcta taattatatc ttccctttgg gactggtctt cttcaccttc tgccaatatt
    40441 cccataggga ttatcgttaa aacaatcatt aagatacaaa caagaattat tcctctgtta
    40501 ttcacgtccc atacccacct tctacgtata gtataccaga ctccgtctat ctgacattag
    40561 atagcataac acattattca atacccatat catatcatct ttcgagtatg gtaaaaagaa
    40621 aaaacaaatg acaagttctt aacttttcct taatattccg attatagagg gggcttccag
    40681 aaatctatca ttcaatattt caatctatag cacgaaggat ttaagtcttt ataaccttaa
    40741 ttgaccagtt gatggtttta ttgcatcaag gttttatccc cgaccctcaa agggaaaact
    40801 gcgattaggg tatgaatatt ataggtgcaa ttttggctta attatttgga ttttgcggag
    40861 ctatacaaaa atagtttcaa aatttctact ataaataaag gcgctatgga taatgcccct
    40921 actattaacc ac
//
LOCUS       DASRNX010000053        23102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_275992,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000053 DASRNX010000000
VERSION     DASRNX010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 23102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23102
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275992"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFD89_02095"
     CDS             <1..225
                     /locus_tag="VFD89_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HZJ57012.1"
                     /translation="RQQEVKILPTNSNEKVVAAARDALNNMKFEIANEIGINLKPGYN
                     GDLTSRQVGHIGGQMTRRMVAMAQQQMSGQ"
     gene            394..945
                     /gene="rsmD"
                     /locus_tag="VFD89_02100"
     CDS             394..945
                     /gene="rsmD"
                     /locus_tag="VFD89_02100"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZJ57013.1"
                     /translation="MRIIAGQSKGMSLMAVKGRNTRPTSEKVKESIFNIIQDRILDSV
                     VLDLFAGTGNLGFEALSRGAKKAFFIDRDIAAVGTIKMNCRNLGYEKQSEIFRSDALV
                     SLTGIAKRDILFDIIFADPPYHYGCEDTLLSAIDEFGILCKYGIVIIEHDAKSPLPDR
                     VSNLYCSDRRKYGGTGISFYRKG"
     gene            953..1432
                     /gene="coaD"
                     /locus_tag="VFD89_02105"
     CDS             953..1432
                     /gene="coaD"
                     /locus_tag="VFD89_02105"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZJ57014.1"
                     /translation="MKIGVYPGSFDPVTEGHLDVIRRSAKIFDKVVVAIARNYSKKPL
                     FTAEERMGLIQRSLNDISNVEVDSFEGLLMDYVHSKNAQVIIKGLRAISDFEYEFQMA
                     LMNRKLDSRVETIFMMTSYKYSFLSSSMVKEVASLGGCITGLVPDHIIPDIFRKLNQ"
     gene            1452..1898
                     /locus_tag="VFD89_02110"
     CDS             1452..1898
                     /locus_tag="VFD89_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZJ57015.1"
                     /translation="MNILSLLDVLEDELDRGTSIPFGNRVLINKGKCLEIIKDIRLNM
                     PEEIKQAEWIKMERQKILVDAQKESEAIIRDAEGQIESLIEENEITQRAYQQAREIME
                     SCQDNAKEIRLGTREYADNLLEEVESYLIEQLDVLKRNRQELDSMK"
     gene            complement(1928..3103)
                     /gene="ylbJ"
                     /locus_tag="VFD89_02115"
     CDS             complement(1928..3103)
                     /gene="ylbJ"
                     /locus_tag="VFD89_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HZJ57016.1"
                     /translation="MFAFASLVIIILVIAYPKQSLSASLKGLDTWLNIVLPALLPFFI
                     VSEIMIELGVVDLLSTLLNPIMRPIFNCPGHSSFVWIMSITSGYPTGPKLIASLYKEG
                     SITKDEGQRMLAFCNNSGPLFMIGAVGIGILNNPEAGKVIAVSHLLGSLILGILFRFY
                     GKGNLEKGRAYTPNRSGESVFKHHAKADIQDNKPFGRILGESVQNCMEAQLIIGGFII
                     LFSVIINLLIQNGLTRGPGYHLGGWDSIYPGFLQLVKPIIAGTLEITTGCQLLGETFL
                     PFNSKLIAASLIIGWGGFSIHSQAISFLANSDFSTGIYLLSKLLHGFFSAIITYTILA
                     FYRIETVATFSSPVYLFNTTVRSSFIASIYMLGSAVFILIATFLLVVIIRSIGSKGR"
     gene            3198..4001
                     /locus_tag="VFD89_02120"
     CDS             3198..4001
                     /locus_tag="VFD89_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZJ57017.1"
                     /translation="MKDDPKIGLALGAGASRGLAHIGVLQALEEHGVIPDLIAGSSIG
                     AIIGALYAAGISPKMLEGISENLDLKPYYDVGIPRLGFIKGEKIEELIRLLTKGKTFD
                     QLRIPLSITAVDLKTNQGVILNEGLVYKAVRASISIPGIFVPVFHGDQVLVDGGLLER
                     LPTKVVRQMGADIIIGVDVGFRGQHDDATNILGIIFQSFEVMELAIVNTGFHEDDIHI
                     YPDLADINPLSFERVGDCIEEGRRVTLEAIDEIKERIRSFKTKPLQGPG"
     gene            complement(3976..5241)
                     /locus_tag="VFD89_02125"
     CDS             complement(3976..5241)
                     /locus_tag="VFD89_02125"
                     /inference="COORDINATES: protein motif:HMM:NF017452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HZJ57018.1"
                     /translation="MNKVLGIIAEYNPFHNGHLYHLQKAIDSVEPDFTIAVMSGNFTQ
                     RGEPAIIDKWARTQMALENGIHLVLELPALYASQTAEIFASGAVQLLHKTGLVTHIAF
                     GSEHNNLYELDLAAGILVDEPAEFQTLLRSNLQKGLSFPLARMRAMQEYFSDTNLAET
                     LLPEVIDNIFSGSNSILALEYLKALKTLKSPMTPVIIPRVGASYNNPDLEGLFPSATA
                     IRGAIHSGMGWESIAAALPKSSLNILQKAVNMGRGPISIKSLAQLLLGLLRRSDTEEI
                     ADWMDVEEGLENRIKKYALECGSIEDFLNQVKTRRYTYTRLQRILIHGLLGIKTDDIS
                     YFEKSGGPRYLRILGFNTGSTSLLADLKKASKLPIISRASNYYRFRDKDLNRMFQYDI
                     LAGDLYALAFSDQDNRHGSQEFTRGLVMV"
     gene            5752..6741
                     /gene="pta"
                     /locus_tag="VFD89_02130"
     CDS             5752..6741
                     /gene="pta"
                     /locus_tag="VFD89_02130"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HZJ57019.1"
                     /translation="MGVISDIIRKAKACKKTIVLPESDDLRMLEAGQRVVSNGIADIV
                     LLGNEEAIKEKAGSIDLCGITIIDPLNSVNADTYARELTELRKHRGMTLDEAKETIKD
                     PLYFGCMMIKLGDADGMVAGAVNSTPDVWRPVLQIIKTRPGISIASSCFLMEVPDCQY
                     GENGVFIYADCGLNPDPTAEGLAAIALSAAESARILADMEPRVAMLSFSTMGSASHES
                     VEKVSKATRLAREMAPDLLIDGELQVDAALDSIIGQQKSPGSPVAGRANVLVFPDLGA
                     GNIAYKLTERLAKATALGPLSQGLAKPVNDLSRGCTVDDIVNVVAITALEAQN"
     gene            6758..7960
                     /locus_tag="VFD89_02135"
     CDS             6758..7960
                     /locus_tag="VFD89_02135"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HZJ57020.1"
                     /translation="MKILVINAGSSSLKYQLINMKDESVLAKGNIERIGMAEPLLKHT
                     PQGRDPIEIHGDFKDHVAAIKKVIETLVHDKYGVIEGMSEIIAVGHRVVHGGESFSSS
                     VVIDDKVMKAIEANVELAPLHNPANIMGINACRNVMPDTPMVAVFDTAFHQTMPQKAF
                     MYAIPYNTYEEYGVRRYGFHGTSHKYVSLKAAELLKRPIDSLKIITCHLGNGSSIAAV
                     DCGKSIDTSMGFTPLEGLPMGTRSGSIDPAILSYLMDKRQMTCEEVNGLLNKESGMLG
                     LSGVSSDFRDLHKAAAEGNMRAQLALDVFNYEVKKYIGAYAVAMGGVDCIVFTAGVGE
                     NTPKVRTRACQGLEFMGVKIDEERNQLALGRLDKDGDISTEDSTVKVLVVPTNEELMI
                     ARETLEII"
     gene            8166..8687
                     /locus_tag="VFD89_02140"
     CDS             8166..8687
                     /locus_tag="VFD89_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HZJ57021.1"
                     /translation="MVIDISDILMEVGSSKDFDGEISIEDIVYQGENIRFDKPLRIEG
                     TVVNADEFIMLSGHLYGYATLQCGVCTEPYDCIADYDLQVNLKPLPDEEDPDIYVYVN
                     DLIHLDDIIIREFILRLPTQRRCSEECRGLCPFCGVNLNREECHCTDEDHQIIDSRLE
                     KLKDFYVNRDREV"
     gene            8693..8872
                     /gene="rpmF"
                     /locus_tag="VFD89_02145"
     CDS             8693..8872
                     /gene="rpmF"
                     /locus_tag="VFD89_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZJ57022.1"
                     /translation="MAVPKRRTSKARRNKRRANWKVSLPGMTECPQCGTIKLSHRVCR
                     ECGFYNKKQVISNED"
     gene            9127..9720
                     /gene="fapR"
                     /locus_tag="VFD89_02150"
     CDS             9127..9720
                     /gene="fapR"
                     /locus_tag="VFD89_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HZJ57023.1"
                     /translation="MKGVLPKKERQRQLIEDLNLDPFMTDEELSELFNVSIQTIRLDR
                     LELGIPELRERIKNVAERNYSKLRSIEGKEIIGELVDVELGKSGISILNTTDDMVFQR
                     SRVVRGHYIFAQAESLALAVIDARVALTGVANIKYKIPIYAGDKLVAKAEVVRRRGNK
                     HFVWVKIRVRQEEAFRGKFILVSIGPDGTDNESGGNE"
     gene            9720..10724
                     /gene="plsX"
                     /locus_tag="VFD89_02155"
     CDS             9720..10724
                     /gene="plsX"
                     /locus_tag="VFD89_02155"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HZJ57024.1"
                     /translation="MKIIVDAMGGDHAPAEVLKGCVGAASEYQVRLTLVGREDIIKQQ
                     LQALGAPLESFEIVNASEVIEPDETPVTAIRRKKDSSMVKAFEILRRNPEGVFISAGS
                     TGALMAGGLLRVGRIKGINRPALAPLIPTKGKPFLLIDAGANAECKPENLVQFAIMGS
                     VYMEQVLNRRNPTVGLVNIGSEEGKGNDLTKETYIRLKEQGNINFFGNIEARDIPAGI
                     VDIVVCDGFTGNIILKLTEGLATNLFSIMKEEFTKGFIPKIGALLLKPSLRGLKDSMD
                     YAEHGGAPLLGINGGIIKAHGSSDARAIKNAIRQGKRFIEQKVLDNIKSKIIGDNEEV
                     "
     gene            10726..11721
                     /locus_tag="VFD89_02160"
     CDS             10726..11721
                     /locus_tag="VFD89_02160"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZJ57025.1"
                     /translation="MGTLFRGGIIGTGSYVPENIVTNKDLEKIVDTTDEWIVTRTGIR
                     ERRISDTNMATSDMAKIAATRAIENSGLTPGDIDLVIVATVTPDNNVPSTACIVQHGI
                     GAINAAALDMNAACSGFIYGLVTANQFIATGMYKNILLIGAESLSKFTDWDDRNTCIL
                     FGDGAGAAVIGRVDEGYGILSQYLGADGSGGDLLRIEAGGSRKPASLETIEQKLHYFY
                     MDGSEVFKFAVRIMASASTEVVRLAGLTNDDVDLLVPHQANIRIIEAARKRLRLDKER
                     VFVNLDRLGNMSAASIPVALDEAARSGFTKKGDNTLIVGFGGGLTWASAMMKWAY"
     gene            11732..12667
                     /gene="fabD"
                     /locus_tag="VFD89_02165"
     CDS             11732..12667
                     /gene="fabD"
                     /locus_tag="VFD89_02165"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZJ57026.1"
                     /translation="MDSLAFVFPGQGAQYVGMGKELYDNFHSVKDLFHKAGDILDVDI
                     TDLCFNGPETELLKTENTQPAILLVSMAAMTVLREHGYSPKMAAGLSLGEYGALTAAG
                     ALTLEDALPLVRMRGQFMQQAVPLGVGAMAAIIGLAAEKVEECCRLARDEGEVNPANY
                     NCPGQIVVSGHKPAVEKVCALAEDLGAKRALVLPVSAPFHSVLLKPAGQRLEQELEKV
                     RIESPVIPVVSNVHASVESRPEGIRENLVLQVSSPVKWEESVRYMMDQGIDTFVEVGP
                     GRALNGFLKKISRDIRGYNIEDMASLEKTLTGLGA"
     gene            12670..13413
                     /gene="fabG"
                     /locus_tag="VFD89_02170"
     CDS             12670..13413
                     /gene="fabG"
                     /locus_tag="VFD89_02170"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HZJ57027.1"
                     /translation="MDLSGKVALVTGASRGIGRAIAIRLAQLGASVGINYSSNADAAE
                     KVVRIIEEGGGRAIAVSGDVSDSDSVAKVIARIDEEWGTLDILVNNAGITRDGLVMRM
                     KEEDWDRVMDINLKGIFNCTKAVSRIMMKKRSGKIINISSVIGIAGNAGQSNYAAAKA
                     GIIGFSKSVAKELASRNVQVNVVAPGFIDTDMTRVLSGEIRDGILSNIPLGRLGSPMD
                     VADLVAFLASDCSSYITGQVINVDGGMIM"
     gene            13509..13736
                     /gene="acpP"
                     /locus_tag="VFD89_02175"
     CDS             13509..13736
                     /gene="acpP"
                     /locus_tag="VFD89_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZJ57028.1"
                     /translation="MSFDKIKEIIVEQLGVEADEVTMEASFIDDLDADSLDIVELIMA
                     LEEEFDMEIPDEEAEKISTVGDVINYIKSHS"
     gene            14000..15241
                     /gene="fabF"
                     /locus_tag="VFD89_02180"
     CDS             14000..15241
                     /gene="fabF"
                     /locus_tag="VFD89_02180"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZJ57029.1"
                     /translation="MPKRVVITGLGAITPLGNDVDTFWDSLKQGKNGIGKIDRFDVEN
                     FSAKMAGLVKDFDPLSYIDRKEARRMDRFTQLAVAASCAAVEDAALDSKNIDAHRFGI
                     ILGTGIGGLGTLEDQANVLFTKGPNRVSPFFIPMMIGNMAAGQISIAVGAKGINTTVV
                     TACASSTNAIGEAFKAIRFGDADIILTGGCEATITPLALAGFCSMKALSTRNDDPQAA
                     SRPFDAHRDGFVMGEGAGILILESYDHAVDRGANILAEIVGYGSTADAYHITAPAPGG
                     EGGARAMKDAIRYAGIEPSVIDYINAHGTSTPYNDKFETAAIKTIFGDHAYRLKVSST
                     KSMTGHLLGAAGGVEAIAVVKTLEEQYIAPTINYTTPDPECDLDYVPNIGLRADVEYA
                     MSNSLGFGGQNASIVLKRFAE"
     gene            15398..16099
                     /gene="rnc"
                     /locus_tag="VFD89_02185"
     CDS             15398..16099
                     /gene="rnc"
                     /locus_tag="VFD89_02185"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZJ57030.1"
                     /translation="MRKIPNQNIGELEREIGYKFQNPKLLLNALTHSSYANEKRGKYP
                     DNERLEFLGDAVLGMVIADYLYKLFKKHKEGDLTKIRASIVSEEPLGEIARDIGLGQF
                     LFLGRGEANTGGRDRDSILADAVEAIIGAVYLEGGIKGAEELIIGLFTPLMERSFQGK
                     GFQDYKTDLQEELQKSDGGEISYRVVKESGPDHDKEFVIEVRYGRRVIGIGKGRSKKE
                     AEQRAAEEALGKIHR"
     gene            16202..19843
                     /gene="smc"
                     /locus_tag="VFD89_02190"
     CDS             16202..19843
                     /gene="smc"
                     /locus_tag="VFD89_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZJ57031.1"
                     /translation="MHCSRNCLVGHFYDDAQDKRCILLLLKRLEMYGFKSFADRIQIE
                     FDKGITAIVGPNGSGKSNVADAVRWVLGEQSAKSLRGSKMEDIIFSGTQERRPLGFAE
                     VSLTLDNLHGAIPVDFAEVTITRRVFRSGESEYYINRSPCRLRDITELFMDTGVGKEG
                     YSIIGQGRIEGILSTQPENRRQIFEEAAGIVKYKSRKQESERKLASVRENIIRIHDVL
                     GEISHQLGPLEEQSKAAREYLDLRDKLKFYDLNRFIMEYDSHREKVDQIKNNVALLAQ
                     DISNHRTDIRNQETKNQELNGRLTGVDEQIQNLAHERYEKSNEIERLTGKVEVIKERI
                     SQLDREKSRLEKEIAEEGHTISKKEEARVAALRDLEKEKEKQETVLLKSTELNIELEK
                     IQRELASSHLESQQKKSEQMEIWNTLSQAKNSITKYEALKSNLEEQRLDSDVRVEELI
                     EDRTALRRRHMENERLVDELLEQEKIKKQERIILEQKKACLKQELEVMDDRLQLQKQQ
                     LEGHKSRLKLLVDMEKTYDGFQKTVRNILVACRNNSVLEKRVCGVIAELIDVPREYEV
                     AIETALGASLQHIVTHDEQDAKYMIELLRQKKWGRATFLPISSIQPRFLNERERGALS
                     LEGCLGRGTDLVAYEPKYADILSNLLGRIIIMKTLDQAVDIAKRFSYSFRIVTLEGDI
                     INAGGSMTGGSLHRRSAGIIGRGREISDLKARITSLVKDMKSTALTRETHLVEYNKLA
                     DALRIMSEEIHELEIAKATGSESLEGTRNQIEIMDKDIQRLSANNNNMAQEIERLQES
                     IGEQENLIEGLETQIERIQATVLNTDSTIEDINRRKGQVDEGITKVRLEMAGLEHEIG
                     HLGDKIHQLDGEISLHTQSLDAKSKVVDDNSDTAIVCAREINGYIEKIGRLSREVEKD
                     GERLTQKQSHKETLAQEISGIERRIKELAKIVDEIQERRHGMDIQLSRTETELENIQN
                     NIWQEYEVTYANALGYRDNQLGPARVKREIHEIKQRIDRLGDVNVKAIEEYKRIKERF
                     DFLTRQKDDLTEAKDNLLSVISDITSTMEQRFIKEFAIINDHFNGVFRRLFGGGKAGL
                     VLEDPDDVLASGIEIIAQPPGKKLQNINLLSGGEKSLTAIAILFAILEHKPAPFCVLD
                     EIEAALDESNVQNFGKFLKEFCERTQFVLITHRKGTMENSDVLYGVAMEEKGVSRMIS
                     VRLEDEIAS"
     gene            19885..20811
                     /gene="ftsY"
                     /locus_tag="VFD89_02195"
     CDS             19885..20811
                     /gene="ftsY"
                     /locus_tag="VFD89_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZJ57032.1"
                     /translation="MNKENDKKTGFFSRLIKGLDKARQGFTTRVDELIKYYREIDDGF
                     FDELEEILITADVGVNTTVEIVDELRDLVKEKKIGDAERIRNLLKEKIAAILTGTEEF
                     EVTPPAVCLVVGVNGVGKTTTIGKLSGLYKRQGRSVLMAAGDTFRAAATDQLQIWGDR
                     AGVQVVSQDEGSDPAAVIFDAVQAARSRHIDVLLCDTAGRLHSKKNLMNELGKINRII
                     DREYPEAQKEVFLVLDATTGQNAITQARLFQEVVGITGIILTKLDGTAKGGIIVAIRS
                     ELDIPIRFIGVGEQIDDLQPFVPQDFVDALFG"
     gene            21163..21549
                     /locus_tag="VFD89_02200"
     CDS             21163..21549
                     /locus_tag="VFD89_02200"
                     /inference="COORDINATES: protein motif:HMM:NF016205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HZJ57033.1"
                     /translation="MEKFTHLGMLFEIYGGLLTHRQRDVMDLYYNHDLSLAEIAEQLS
                     ISRQGVHDLVKRSGLSLENAENNIGFLKFRGGIFHGINDIERDLDRLLSCLGQGAEGE
                     GIEKEPHELKRKLSLIKDKIMRIGDL"
     gene            21598..22929
                     /gene="ffh"
                     /locus_tag="VFD89_02205"
     CDS             21598..22929
                     /gene="ffh"
                     /locus_tag="VFD89_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZJ57034.1"
                     /translation="MAFEGLSSKLQSTFKKLTGKGKLSEKDVKDAMREVRLALLEADV
                     NFKVVRDLVKRIGDRAVGQEIMRSLTPGQQVIKIVNEELTDLMGGTYTRIATADKPPT
                     IIMMVGLQGAGKTTTAGKLGGYFKKEGKNPLLVACDIYRPAAIKQLQVVGDQQSIPVF
                     EMGKDDPVRIASKAVAHAKDQGYDIVIVDTAGRLHINEELMNELSNIQEAVKPQEILL
                     VVDAMTGQDAVNVAQDFDERLEIDGVVLTKLDGDSRGGAALSVRAVTGKPIKFAGMGE
                     KLTDLELFHPDRMASRILGMGDVLSLIEKAQANIDTEKALELERKLRTQQFTFDDFLE
                     QLQQLKNMGPLSDIVGMIPGMNPGKLGGVNLDDRQLVRTEAIIQSMTKEERFNPGIIN
                     ASRRKRIARGSGTNIQDINKLLKSFTEFRKMMKRMSGMEKSMKRGKFKFPF"
BASE COUNT         7049 a         4186 c         6080 g         5787 t
ORIGIN      
        1 cgacaacagg aggtgaagat attgcccaca aatagtaatg aaaaagtagt tgcagcagct
       61 agggatgcac ttaacaacat gaaatttgag attgccaatg aaataggtat taacttgaaa
      121 ccagggtata acggggattt aacttcaaga caggtagggc atataggcgg ccagatgaca
      181 aggagaatgg tcgcgatggc tcagcaacag atgagcggtc aataataaat attgataata
      241 attaaaagga acgggtagat tatccgttcc ttttaatgtt gggtccatta gttgtccaac
      301 atttttctat aatacttgtt acacattttt atctatgata aaatgataac taaccttggg
      361 ggtattagca tgtaataatt agggggaaat tttttgcgca taatagccgg acaatcaaag
      421 ggcatgtcac ttatggctgt gaaagggaga aacacaaggc ctacctcgga aaaggtgaag
      481 gaatctatat tcaacattat acaggatagg atcctggata gtgtggtgtt ggaccttttt
      541 gccggtaccg ggaatttggg ttttgaagcc ctcagcaggg gtgcaaaaaa agcgttcttt
      601 atcgatagag atatagcagc cgtgggaacc attaaaatga attgtcggaa tctaggttat
      661 gagaaacagt ccgagatatt tagaagtgat gctttagtat cattgactgg tatagccaaa
      721 cgtgatattc tttttgatat aatctttgca gatccgccgt atcattacgg gtgtgaggac
      781 accttattat ccgctataga cgaatttgga attctatgca aatatggtat tgttatcata
      841 gaacatgatg ctaaatcccc attacctgat agggtctcca atctctactg ctctgacaga
      901 agaaaatatg gtgggaccgg aatcagtttt tataggaaag gatgaggcat acatgaaaat
      961 aggtgtgtat cccggcagtt ttgatccggt gactgaggga cacttggacg ttattagacg
     1021 gtcagccaag atatttgata aggttgtagt ggcgattgcc agaaattata gcaaaaaacc
     1081 tctctttacg gcagaggaaa ggatgggact gatccaaaga tcccttaatg atatctccaa
     1141 tgtagaggtt gacagcttcg aaggcttgtt aatggactac gtacatagca aaaatgctca
     1201 ggtaatcata aagggattgc gggctatatc ggattttgaa tatgaattcc agatggcgct
     1261 gatgaacaga aagctggatt ctagagtaga aacaattttt atgatgacca gttataaata
     1321 ttctttcttg agttccagca tggtcaaaga ggtcgctagc ctaggcggat gtataaccgg
     1381 tttagtcccg gaccatataa tacctgatat ttttcggaaa ttaaatcaat aaaaagatgg
     1441 ggggattatc catgaatatc cttagtttgt tggatgtgtt agaggatgaa ttggataggg
     1501 gaacaagcat accctttggg aatagggtac ttataaataa ggggaaatgc ctggaaatta
     1561 taaaggatat tagactgaat atgccggagg agatcaagca ggctgaatgg atcaagatgg
     1621 aaaggcagaa gatccttgta gatgcccaga aagaatccga ggcaattata cgggacgccg
     1681 aagggcagat agagtctttg atagaagaaa atgaaattac ccaaagggcc taccaacagg
     1741 cgcgagagat catggaaagc tgccaggata acgccaaaga aattcgttta gggaccaggg
     1801 aatacgcaga taacctgctg gaagaggtag aaagctatct gattgagcag ctggatgttc
     1861 tcaaaagaaa tcgtcaggaa ttggactcca tgaaataagg tatctgtttg atgaccggcc
     1921 gggtatatta tcggccctta cttccaatgg atcttataat aaccactaat agaaatgtgg
     1981 ctatgagtat aaacacggct gatcccagca tatatataga cgcaataaaa ctactgcgta
     2041 ccgtcgtatt gaaaagatac acggggctgc tgaaggttgc aacggtttcg attctataaa
     2101 acgccagtat ggtgtatgta ataatggctg aaaaaaagcc gtggagcagt tttgataaaa
     2161 gatagattcc cgtggaaaaa tcgctgtttg ccaaaaagct gatagcttgg ctatggatag
     2221 agaatccgcc ccagcctatt ataagcgatg cggctatcaa cttgctgtta aaagggagga
     2281 aggtttcccc taataattgg caacccgtgg tgatctccaa ggtcccggct attataggtt
     2341 ttactaattg gaggaaaccc gggtatatag aatcccaacc gcccaaatga tagcccggtc
     2401 ctcgggttaa tccattttgt atgagcaaat ttataattac tgaaaacaat attataaatc
     2461 cgcctatgat aagttgcgct tccatgcaat tttgaaccga ttcacccaga attcttccga
     2521 agggcttgtt gtcttggata tcggcctttg cgtgatgctt aaaaacggat tccccgcttc
     2581 ggttcggagt atatgccctt cctttctcaa ggtttccttt gccataaaat cggaatagga
     2641 tgcctaggat tagagacccc agcaagtgac tgacagcgat gaccttccca gcctccggat
     2701 tattcaaaat ccctatcccc actgccccga tcataaacaa cggaccggaa ttgttacaga
     2761 aagcaagcat acgttgtccc tcgtctttcg taattgaccc ttccttatat aaggaggcta
     2821 tgagtttggg tccggtggga tatccggagg taatgctcat tatccataca aaggaactgt
     2881 ggcctgggca attaaaaatc ggtctcatta tagggtttaa gagtgtagat aacagatcga
     2941 caactcccag ttctatcatg atctctgata caataaaaaa gggaagcaag gcgggcaata
     3001 ctatatttag ccatgtatca agccctttca aagaagcaga aagtgattgt tttggatagg
     3061 ctattacgag tataataata acaagggatg cgaaggcaaa cattgacttt tgaaataatt
     3121 tatattttga agacaagttt gattatcccc cctaaacgct tgatactagt ctattatatt
     3181 tggggagggg gttaattatg aaggatgacc caaaaattgg tttggcttta ggtgcgggtg
     3241 cctccagggg cctggcccat attggagttt tgcaggcgtt ggaagaacat ggtgtcatcc
     3301 cggatctgat agccgggagc agtatcggcg ctataatcgg ggccctttat gccgcgggta
     3361 tttctccaaa gatgttggag ggtatatcag agaacttgga tttaaagcca tattatgatg
     3421 taggtatacc gagattgggc tttattaaag gcgaaaaaat tgaggagctc attagactcc
     3481 ttactaaagg aaagaccttt gatcagctta ggatacccct tagtataacc gctgtggatt
     3541 taaagactaa ccaaggggtg atattaaacg aggggttggt ctataaggca gtcagggcga
     3601 gcatctccat ccccggtatc tttgtacctg tattccacgg tgatcaggtc ctagtagacg
     3661 ggggtttatt ggagcgcctg ccgaccaagg tggtaagaca gatgggagcc gacattatta
     3721 taggggtgga tgtggggttt agggggcagc acgatgatgc caccaacatc ctaggcataa
     3781 tatttcaatc ttttgaagta atggaattgg ccatagtaaa caccggcttt catgaggatg
     3841 atatacatat ctatcccgat ctggccgata taaatccatt atcctttgag agggtagggg
     3901 attgtatcga ggagggaaga agggtaaccc ttgaggcaat agacgagatt aaggaaagaa
     3961 tacgttcatt taagactaaa ccattacaag gccccgggtg aattcttggc tgccatggcg
     4021 gttgtcttga tctgaaaacg ccaaggcata aagatccccc gcaagaatat catattggaa
     4081 catcctattc agatccttgt cccgaaatct gtaatagtta gaagccctag agataatagg
     4141 caatttggat gcttttttaa gatcagccaa aagggaagta gatccggtat taaagccgag
     4201 gatgcgcaga tagcggggac caccgctctt ttcaaaatag gagatatcat ccgtttttat
     4261 tcctaaaagg ccatgaatca gtatgcgctg cagcctggta taggtgtagc gccttgtctt
     4321 gacctgattg agaaaatcct ctatgctgcc acactccaaa gcatattttt ttatgcgatt
     4381 ttccagccct tcctccacgt ccatccaatc ggctatttct tcggtgtctg atcgtctcag
     4441 caatcccaac agcagttggg ctagggactt aatggatatg gggccgcggc ccatattaac
     4501 ggctttctgt aatatgttaa gggaagattt gggcagtgcg gcagcgatgg attcccaacc
     4561 cataccggag tgtatagcgc cgcgtattgc agttgcgctg ggaaacaatc cttccagatc
     4621 cgggttgtta tatgaggcgc ctaccctggg gattataacg ggcgtcatgg gactttttag
     4681 tgtcttaagg gcctttagat actccagagc aaggatagaa ttggaaccac tgaaaatatt
     4741 gtcaattacc tctggtaata aggtctctgc aaggttggta tccgaaaaat attcttgcat
     4801 ggccctcatg cgcgcaaggg gaaaggaaag gcctttttgc agattcgacc ttaaaagtgt
     4861 ttgaaattct gcgggttcat caaccaatat ccccgcagct agatccaatt catataggtt
     4921 attatgttcg ctgccaaagg ctatatgggt aaccaagccg gtcttatgca gcaattgtac
     4981 tgcgccggaa gcgaagatct ccgctgtttg ggaggcatac agggcaggaa gttctaaaac
     5041 caaatggatc ccattttcta gggccatttg tgtgcgggcc cacttatcaa taatggcggg
     5101 ttcacctcgc tgggtgaagt tacctgacat tacggcaatt gtaaaatcgg gttctacgga
     5161 atctatggct ttttggagat gatacagatg tccgttatgg aaggggttat attcggctat
     5221 tatgcctaat actttattca aggctatcat tccttcataa taattggggg ttcttatttt
     5281 tgcaggtaca caaaagtgat ccgcttttaa atcagcctct agagtttgat cccgggaatg
     5341 tcccattagg aatggaatag gttccgactt tcctatggat ggtgacccta aagagacggg
     5401 gcaccgggac tataatttct gagcctgttg aatataataa ttatatatat tggaggcgtt
     5461 ttattcaagg cagtaatata gataaacaat ggattaatta aatttatcgg catgatagtt
     5521 gcaatgggtc tgtgcaatat tacggagata aattttatag ttgaagttga ttagttgttt
     5581 atctgtagaa tgcattacag ataaaggtat tattgagatc tcgaatttaa tcatatatca
     5641 tataattgaa attgattaga ggattttggc gtttgatgta gaatagatag atggttggat
     5701 accacttggt attcaggcga gtatcaaaaa ataagacaga ggagtttttt aatgggcgta
     5761 ataagtgata ttataaggaa ggcaaaagct tgtaaaaaaa ctatagtatt acctgagagt
     5821 gacgatctgc ggatgttaga agccggtcaa agggttgtaa gcaatgggat agccgatata
     5881 gtattgttgg gtaacgaaga ggctatcaag gaaaaagcag gtagcattga tttatgtggg
     5941 attactatta tagatcctct aaattctgta aatgccgaca cttatgctag ggagttgacg
     6001 gaacttagaa agcatagggg catgaccttg gatgaggcga aggagactat aaaggatccc
     6061 ctttattttg ggtgcatgat gatcaaactg ggagatgccg atggtatggt agccggtgct
     6121 gtaaatagta ctcccgacgt atggaggccg gtactacaga ttattaagac tcgtcccggt
     6181 atttctatag catcaagttg ttttcttatg gaagttcctg attgccaata tggcgaaaac
     6241 ggtgtgttta tctacgcaga ctgcgggctc aatccagatc ccacagccga gggactggca
     6301 gcaatcgctc tcagcgcagc agagtcggca aggattctgg ccgatatgga gccaagggta
     6361 gctatgttgt ccttttcaac gatgggcagt gcaagtcacg aatccgtaga aaaagtttca
     6421 aaggcaacca ggctggccag ggaaatggcc cctgacctat tgatcgacgg tgaacttcag
     6481 gtggatgccg ccttggattc tatcatcggg caacaaaaat ccccgggaag tcccgtggca
     6541 ggaagagcca acgtgttggt ttttccagat ttaggggccg ggaatatagc ctataagctc
     6601 actgagaggt tggcaaaggc tacggcgctg ggacctctaa gccagggatt ggcaaaaccg
     6661 gtcaacgact tatccagggg atgcacagtt gatgatatag tcaatgtagt tgccattacg
     6721 gctttagagg cacagaatta agggaggaga gttcaatatg aagatactag ttattaatgc
     6781 cggtagttcg tcgctcaaat accagcttat aaatatgaag gatgaaagcg ttttagccaa
     6841 gggcaatata gaaagaattg gtatggcaga gcccctgctt aagcataccc cccagggcag
     6901 ggatcccatt gaaattcatg gagactttaa agaccatgta gcggctataa aaaaagtgat
     6961 cgagaccctg gtccatgata aatatggtgt gatagagggt atgagcgaaa taatcgccgt
     7021 cgggcatagg gttgttcatg ggggcgagag tttttcaagt tcggtggtca ttgacgataa
     7081 agtaatgaag gccatagagg caaatgtgga gctggctccc cttcataacc ctgctaacat
     7141 aatggggatc aacgcctgca ggaatgtaat gccggatacg ccaatggtgg ctgtttttga
     7201 taccgcattt caccagacca tgccccaaaa ggcttttatg tacgctattc cctacaatac
     7261 ttatgaagaa tatggggtaa ggcgctacgg ctttcatggg acttcccata aatatgtatc
     7321 cctcaaggca gcggaattgc taaaaaggcc catagactcc ttaaagataa tcacttgcca
     7381 tttggggaat ggatccagta tcgcggcagt tgactgcggg aaatccatcg ataccagtat
     7441 gggttttact cccttagaag gacttcccat gggcaccaga agcgggagca tagatcctgc
     7501 tatcctcagc tatctcatgg acaaaagaca aatgacctgt gaagaagtaa acgggctctt
     7561 aaataaagaa tcaggcatgc tgggattgtc aggggtcagc agcgacttta gagatctgca
     7621 taaggctgcg gcggaaggga atatgagggc gcagttggct ttagacgttt ttaactatga
     7681 agtaaagaaa tatattggag cctacgcagt agctatgggc ggagtggatt gtatagtatt
     7741 tactgcggga gtaggggaga atactccaaa ggtaagaaca agagcctgtc aaggattaga
     7801 attcatgggc gtaaagatag acgaagaacg gaatcaactg gcactgggta ggctggacaa
     7861 ggatggagat attagtacgg aggactccac tgttaaggtt ttggtagtac ctaccaatga
     7921 agagctgatg atagccaggg aaaccttgga aatcatctaa tgattattgc ccagaaagta
     7981 ctttagttga accccgtatg ctattagctg aatttaatga actataccgc ctttgataat
     8041 ttcttcattt caaggcgtaa ggcatcccaa tagtgggcgt cccaatagag gatatattcc
     8101 ttgacaaaat taggggcaat ctatataatt aatccgtccg tctacgaaat aatggagtga
     8161 tagatatggt tatagatatt tcggacatct tgatggaagt aggctcatcc aaggattttg
     8221 atggggaaat aagtatcgag gatatcgttt atcaaggcga gaatatccgc tttgacaaac
     8281 ccctccgcat agagggtacc gtagtaaatg ctgatgaatt cattatgcta agcggccacc
     8341 tatatggcta tgcaacccta caatgtggtg tatgtactga accatatgat tgcatcgcag
     8401 attatgactt gcaagtaaac ctgaagcccc tgcccgatga agaggatccg gatatttatg
     8461 tttatgtaaa cgatcttatc catttagatg atatcattat tcgtgaattt attctcagat
     8521 tgccgacgca gagacggtgc tctgaagaat gtagagggct ttgtccattt tgtggtgtca
     8581 atttaaacag agaagaatgt cactgtacgg atgaagacca tcaaatcata gatagtaggc
     8641 tagagaaatt aaaagacttt tatgtaaaca gggacaggga ggtgtaacag ccatggcggt
     8701 gccaaagaga agaacatcta aagctagaag gaataagcgt agggctaatt ggaaggttag
     8761 cttgcctgga atgacagaat gtccacaatg tgggaccatt aaactttccc atagggtgtg
     8821 cagggaatgc ggattctaca acaaaaagca agtaatttcc aatgaggatt aatggcttaa
     8881 tactttgttg cgttatctat ggaaaaacaa taaagcatcg attaaaaact accttatggg
     8941 attagggatc agaccccatc cacctcgggt agttttttgt ttttcaaaaa ggtattgcat
     9001 aatagtatta aatattatat actaccatta gtaccagctc ctagtataat gttaaaatat
     9061 cagaagccac cgcctgagat atttatgggt gataaacaga atatacaaaa aggtgattaa
     9121 tagatgataa aaggtgtttt gcctaaaaaa gagcgccagc gacaattgat tgaagatttg
     9181 aacctggacc cctttatgac cgatgaggaa ttgagcgagc tctttaacgt cagcatacag
     9241 actatcagac tggatagact ggaactcggt ataccggaac tgagggagcg gataaaaaat
     9301 gttgcggagc gcaactacag caagcttaga tccattgagg gcaaggaaat aattggggaa
     9361 ttggttgacg tcgaactggg taagagtggc atatccattc taaataccac cgacgatatg
     9421 gtatttcaac gctccagggt agtaagggga cattacatat ttgcccaggc ggagtccttg
     9481 gccttggctg taattgacgc tcgggtagcc ctgacagggg tagccaatat caagtataaa
     9541 atacccatct atgccgggga taagctggtg gcaaaagccg aagttgttcg gcgacgaggt
     9601 aataagcatt ttgtttgggt aaagattagg gtgaggcagg aagaagcctt taggggaaaa
     9661 ttcatcttgg tttccatagg tccggacggg acagataatg aatcgggagg caacgaataa
     9721 tgaagataat agtagatgct atggggggag atcatgctcc cgctgaagta ctcaagggtt
     9781 gtgtaggtgc tgccagcgaa taccaggttc gtctgaccct agtcggccgg gaggatataa
     9841 taaaacaaca gttgcaggct ctgggggcac ctttggaatc ttttgaaata gtcaatgcta
     9901 gtgaagtcat agaacctgat gaaaccccgg tgacggctat acgccgtaaa aaagattctt
     9961 ctatggtaaa ggcctttgag atcctaaggc ggaaccctga gggggtattt atatcagccg
    10021 gcagcacagg tgcgctgatg gctgggggcc tgttaagggt aggccggata aagggtataa
    10081 acagacccgc cctggcgcct cttattccca caaaaggaaa gccgttcctt ttaatagatg
    10141 ctggagccaa cgcagaatgc aagcctgaaa atttggttca gtttgctata atgggttcgg
    10201 tatatatgga acaagtttta aacagaagaa atcccacagt gggcctagtg aatataggaa
    10261 gcgaggaagg caagggcaat gatctgacca aagaaaccta catacgctta aaggagcagg
    10321 ggaatattaa tttttttggg aatatagaag ctagggatat cccggccgga atagtggata
    10381 ttgtcgtatg cgacggcttt accggcaata tcattttaaa gctcacggaa ggcctagcaa
    10441 ccaacttatt tagcataatg aaagaagaat ttacaaaggg atttattccg aaaatagggg
    10501 ctcttttatt aaaacccagc ctaagggggt taaaggattc tatggactat gcagaacatg
    10561 gaggcgcccc actgcttggg ataaatgggg ggattataaa ggcccatggc agttccgatg
    10621 caagggcgat taaaaatgcc ataagacagg gtaaacggtt cattgagcaa aaggtcttgg
    10681 ataatattaa aagcaagatc ataggtgaca acgaggaggt atagcatggg gacactattt
    10741 cgtgggggta tcatcggcac gggtagctat gttccggaaa acatagttac caataaggat
    10801 ttggaaaaga ttgtagacac tactgatgaa tggattgtga cgcgcaccgg cattagagag
    10861 aggcgcatat ccgatacaaa tatggcaaca tcggatatgg ctaagatcgc agcaacaaga
    10921 gcgatagaaa attcagggtt gaccccaggg gatatcgacc ttgtaatagt agcaacggtg
    10981 acccccgaca acaatgtacc ttctactgcc tgtattgtcc agcatggtat cggagcgata
    11041 aacgccgctg ctcttgatat gaacgcggct tgttctgggt ttatttacgg actggtaacg
    11101 gcaaaccagt ttatagctac gggtatgtat aaaaacattc ttcttattgg tgcggagagc
    11161 ctcagcaaat ttactgattg ggatgacaga aatacctgta tcctatttgg ggacggtgca
    11221 ggtgcggcgg taatagggag ggttgatgag ggatacggca tattgtccca atatctgggt
    11281 gcagacggaa gcggcggcga tctgctgagg atagaggcgg gcggatcgcg taagccggca
    11341 tcgctggaga ccatagaaca aaaactccac tacttctaca tggacgggag tgaagttttt
    11401 aagtttgcgg ttcgtattat ggcctctgca tccacggagg ttgtgaggct ggcagggctt
    11461 accaatgacg acgttgacct tttagtaccg caccaggcca acatcagaat tattgaagca
    11521 gccaggaaga ggttgaggct ggacaaagaa agggtcttcg taaacttgga ccgattgggc
    11581 aatatgtctg ccgcttcaat acctgtggcc ttggacgaag cggctaggtc cggttttaca
    11641 aaaaaaggcg acaatactct catagtgggc tttggcgggg gattgacctg ggcatcggcg
    11701 atgatgaaat gggcatatta aggagacgga tatggatagc ttagcatttg tttttcccgg
    11761 tcaaggggca caatatgtgg gtatgggcaa ggagctgtat gataacttcc atagcgtgaa
    11821 ggatttgttt cacaaggccg gggatatcct agatgtggat attacagatc tttgctttaa
    11881 tgggccggaa acggaattat tgaaaacgga aaatacccag cctgcgatac tcctggtaag
    11941 catggctgct atgacggtac ttagggaaca cggatacagc ccgaaaatgg cggcgggtct
    12001 gagcctgggc gaatacggag ccctgacagc tgcaggggca ttaactctcg aagacgccct
    12061 ccctttggta cgaatgaggg gacagttcat gcagcaggca gtcccattgg gtgtaggggc
    12121 catggctgca ataataggcc tggcggctga aaaggttgag gaatgttgca gactggcccg
    12181 ggatgaggga gaggtcaatc cggcaaatta taattgtccg ggtcaaatag tggtatccgg
    12241 gcataagccg gctgtagaaa aggtctgcgc cctggcagag gatttggggg caaaaagggc
    12301 tttggtgctc cctgtaagcg caccttttca ttctgtcctt ctcaaaccgg cagggcaaag
    12361 gctggagcag gagcttgaaa aagtgcgaat agaatcacct gtaatccctg tagtatccaa
    12421 tgttcatgcc tcagtcgaaa gccgacccga agggatacgg gaaaaccttg tgcttcaagt
    12481 gagcagtcct gtaaaatggg aagagagcgt caggtacatg atggatcaag gtatcgatac
    12541 ctttgttgaa gtaggtccgg gaagagcact caacggattt ttgaagaaga tatctcgaga
    12601 cattcgggga tataatatag aggatatggc atcgcttgaa aaaactttga cgggattggg
    12661 ggcataaaca tggatctatc aggaaaggtc gcccttgtta caggggcttc cagagggatt
    12721 ggcagggcca tagccataag gcttgcccaa ttgggtgcct cggtgggcat aaactactca
    12781 tcaaatgcag acgctgccga aaaagtagtc agaatcatag aggaaggcgg cggcagagcc
    12841 attgccgtat caggggatgt ctccgattct gattcggtgg ccaaagttat agcccggatt
    12901 gacgaagagt ggggaacatt ggatattctg gtgaacaatg cgggtatcac ccgggacgga
    12961 cttgtgatga ggatgaagga agaggactgg gaccgggtta tggatatcaa tctgaaggga
    13021 atctttaatt gtaccaaagc ggtctccagg ataatgatga aaaaacgcag cggcaagata
    13081 ataaacatct catcggttat agggatagcc ggcaatgccg ggcaatcaaa ctatgctgcc
    13141 gctaaggcgg gtataatagg attttcaaag tctgtggcca aagagttggc ctcgcgcaac
    13201 gtacaggtca atgtggtggc cccgggattt atcgataccg atatgaccag ggtgttgtcc
    13261 ggagaaataa gagacggcat tttatccaat atacccctcg ggaggcttgg aagcccgatg
    13321 gatgtggctg atctggtagc ctttttggca tcagattgca gttcatacat tacaggacaa
    13381 gtaataaatg ttgacggtgg tatgataatg taatatcata cgatatatta ttcagctttc
    13441 tgatatttgg aaggcaatgc agcttttcct tgtagtaagg ccgaaagcac tgaagggagg
    13501 tgtaatgcat gagcttcgat aagataaagg aaattattgt tgagcaactg ggagtagagg
    13561 cagatgaggt taccatggag gcatcattta ttgatgattt agatgcggac tcattggata
    13621 ttgttgagct tataatggct ttggaagaag agtttgatat ggaaatccct gatgaggaag
    13681 ccgaaaaaat ttcaaccgta ggcgatgtca tcaattacat taagagccat tcataaggat
    13741 tgaatcatgg ccgggagcct tctgttttgg gaggctccaa atcttctgtc ggcttgctat
    13801 atagcttggt tttggatagt ttcatatatg acctaccggc atttgaataa aataattatg
    13861 tagcagggat ttagaatagt tcgtattacc ggggctatgc cttggggtac ccgacagcgt
    13921 acattttggt attttcttat tgaaattata ttaatacatt ttgattttta atgcgataga
    13981 ctgcatgagg gaggtttcta tgccaaaaag agtggtaatt acaggattag gggctataac
    14041 ccctctggga aacgatgtgg acaccttttg ggattcatta aagcagggta agaatggaat
    14101 cgggaagatc gacaggtttg atgttgaaaa cttttccgcc aagatggcgg ggttggtaaa
    14161 ggatttcgac ccactatctt atatcgatcg caaggaagcc agaaggatgg acaggttcac
    14221 ccagcttgct gttgcagcat cttgtgccgc tgtggaagat gccgcactgg attctaaaaa
    14281 catagacgct catcgctttg ggataatact aggcaccggt atcggcggtc ttggaacctt
    14341 ggaagaccag gctaatgtcc tctttaccaa agggccaaat agggtgagtc cgttttttat
    14401 acccatgatg atcggcaaca tggcagcggg ccaaatctcc atagccgttg gtgctaaggg
    14461 tataaacacc acggtggtta ccgcttgcgc ctcctcgact aatgccattg gagaggcctt
    14521 taaagccatt agattcggtg atgccgatat tattttaaca ggtgggtgtg aggccacaat
    14581 tacccctctg gctttagcgg ggttttgttc aatgaaggcc ctatctacaa gaaacgatga
    14641 tccacaggcg gcatctagac cctttgatgc ccacagggac ggatttgtca tgggggaagg
    14701 ggccggtata ctcatcttgg aaagctatga ccatgccgta gacagagggg ccaatatcct
    14761 ggcggaaatc gtaggatatg gttcgactgc ggacgcctat catatcaccg caccggcacc
    14821 cgggggcgag gggggtgcaa gggctatgaa agacgccatc cgctatgccg ggattgaacc
    14881 ttctgtgata gactatatca atgctcacgg aacttcaact ccctataacg acaagttcga
    14941 gaccgctgct atcaagacca tatttgggga ccacgcctac aggctaaagg taagctcaac
    15001 caaatccatg actgggcatc tgctgggggc ggcagggggg gttgaggcca ttgcggtggt
    15061 aaagacactt gaggagcaat atatagcgcc caccataaac tatactaccc ccgatccgga
    15121 atgcgattta gattatgtgc ccaatatagg gctcagggct gatgtagagt acgctatgtc
    15181 caattccttg ggttttggag ggcaaaatgc ttccattgtt ctaaagcgct ttgccgaata
    15241 aaaagttgga tttttaaaag ggcccccatg ttaaagggtc cttttttgat acccacaaaa
    15301 atgccgaaac aatcataacc tgtatcctct tgcgctacag gataaaaaaa cctataatag
    15361 tcataagaaa aatgggcaga gatagaggag aatattaatg aggaaaattc cgaatcaaaa
    15421 cataggggaa ttggaaagag agattggata caaatttcaa aaccccaaac tcttgctaaa
    15481 cgctctaacc catagctcat acgccaacga aaagcggggt aaataccctg ataatgagcg
    15541 cttagaattt ttaggagacg cagttttagg catggtaata gcggattacc tatataaact
    15601 ctttaaaaaa cataaggaag gcgatctcac caagataagg gcaagcatag tatcggagga
    15661 accactaggt gagattgccc gggatatcgg cttgggacaa tttctattct tgggaagagg
    15721 tgaagccaat accgggggca gggaccggga ttctatatta gccgatgccg tggaagcgat
    15781 tataggggcg gtatacctag aaggcggtat taagggggca gaggagctta taatagggct
    15841 atttaccccc ttgatggaaa ggagctttca gggtaaggga tttcaagatt acaagaccga
    15901 tctgcaagaa gaactacaga agagcgacgg tggagaaata agctacagag tagtgaagga
    15961 aagcgggccg gatcatgaca aggaatttgt tatagaggtg cggtacggga gacgggttat
    16021 tgggataggc aagggaagga gtaagaagga ggcggaacag cgagcagccg aggaagcttt
    16081 agggaaaata cataggtgag gggatatcca taaatggaat atgttcccat ggggggtagc
    16141 agttacaggc attttatgct agaattgatt agcgtgaccg gacgagtaag gaagtcttaa
    16201 aatgcattgt tcaaggaatt gcctagtcgg tcatttctat gatgatgccc aagataagag
    16261 gtgtatatta ttgttattaa aaaggctcga gatgtatggt tttaagtcct tcgcagatag
    16321 aattcagatt gaatttgaca agggtattac agctatagta ggccctaatg ggagcggcaa
    16381 aagcaatgta gccgatgcag tgagatgggt gttgggagaa cagagcgcca aatccctgag
    16441 gggcagcaag atggaggata taatcttttc cggcacccaa gaacgaagac ctttaggttt
    16501 tgccgaggta tccctaacct tggataatct acatggggct attcctgtgg actttgctga
    16561 ggtaaccatc acaagaaggg tttttaggtc cggtgagagc gaatactaca taaaccggtc
    16621 gccttgcagg ctaagggata ttacggaatt gttcatggac actggtgtgg gcaaggaagg
    16681 atactcaatt attggacagg ggcgaattga agggatactc agcacccaac ctgagaatag
    16741 aaggcagata ttcgaagaag ccgcgggtat cgtaaaatat aagagcagaa aacaggaatc
    16801 tgagagaaag ctcgcaagtg tcagggagaa cataattaga atccatgatg tcctagggga
    16861 gattagtcat caattaggac cattggaaga gcagagtaag gcggcgaggg agtatctgga
    16921 tcttcgggat aaattgaaat tctatgattt aaacaggttt atcatggaat atgatagcca
    16981 tagggaaaag gtagatcaga taaagaataa tgtcgcactt ttggctcagg atatatccaa
    17041 tcaccggaca gacatccgaa atcaagagac aaagaatcag gaattgaacg gcaggctaac
    17101 cggggtagat gaacaaattc agaatcttgc ccatgaaaga tatgaaaagt ccaacgaaat
    17161 tgaaagatta acgggtaagg tagaggttat taaggagagg atcagccagc tagataggga
    17221 aaaatctagg ttggagaagg agatagccga ggaagggcat acaatttcaa aaaaagaaga
    17281 ggcacgtgtc gccgccctaa gggatttgga gaaggaaaaa gaaaaacaag agacagtttt
    17341 gctaaaatct acggaactca atatagaatt ggaaaaaata cagagggaat tagcttccag
    17401 ccacctggaa tcacagcaaa aaaagagcga acagatggag atctggaaca ccctatccca
    17461 ggcaaagaat tctataacca agtatgaggc gttaaaaagc aacttggagg agcagagatt
    17521 ggattcggac gtgcgggtgg aagagttgat agaagatagg accgctctca gacgaaggca
    17581 catggaaaat gaaaggttag tcgatgaact cctcgaacag gaaaagataa aaaaacagga
    17641 aaggattatt cttgaacaga agaaagcatg cctaaaacag gaacttgaag taatggatga
    17701 tagacttcaa ctgcagaaac agcagctgga aggacataaa tccagactaa aattgttggt
    17761 ggatatggaa aagacctatg acgggttcca aaaaactgtc aggaatatat tagtggcatg
    17821 cagaaataat tcggtattgg aaaaaagggt ttgcggtgta atagcggagt taatcgatgt
    17881 cccaagagaa tatgaggtgg ccatagagac cgccctaggg gcaagtctgc agcatatagt
    17941 tacccatgat gaacaggatg ctaagtacat gattgagctt ttgaggcaaa aaaaatgggg
    18001 gagggcgact tttcttccaa tctcatcaat acaaccaagg tttctcaatg aacgggagcg
    18061 gggggcatta tctctcgagg gttgccttgg acggggaacc gaccttgtag cgtacgaacc
    18121 taaatatgca gatattctta gcaacttgct aggcagaata attattatga aaaccctgga
    18181 tcaggccgta gatatcgcta aaagattttc atactccttt agaatagtaa cactggaagg
    18241 ggatattatc aatgctgggg gctcgatgac cggcggcagt ctacacagaa gaagtgcagg
    18301 cattattggt cggggcaggg agataagcga tcttaaagcc cgcattacat cattggtaaa
    18361 ggatatgaaa tctaccgctt tgacgaggga aacacacctg gtggaatata acaagttggc
    18421 agatgcctta aggattatgt cagaggagat ccatgaactt gaaattgcta aggcaactgg
    18481 cagtgaaagc ctggagggga ctcgtaatca aatcgagata atggacaagg atatccaaag
    18541 gctgagtgca aacaataata acatggccca agagatagag aggttacaag aatccatcgg
    18601 ggagcaagaa aaccttattg agggattgga aacccaaatt gaaaggatac aggcaacagt
    18661 tctcaatact gattcgacta tagaggatat aaacaggcgt aaagggcaag tcgatgaagg
    18721 gataaccaag gtcagacttg agatggccgg gttagaacat gaaatcgggc atttggggga
    18781 taaaatccat caattagatg gggaaataag cctccacact caatcccttg atgcaaagtc
    18841 aaaggtcgta gacgacaatt ccgatactgc catagtatgt gcacgtgaga ttaacggata
    18901 catagaaaaa ataggaagac tcagcaggga agtagagaaa gacggtgaga ggctaaccca
    18961 gaaacaatcc cataaggaga ctttagcgca agagataagt ggcatagaaa ggcgaataaa
    19021 ggagttggct aagatcgtcg atgaaatcca ggaacgaagg catggcatgg atatccaatt
    19081 atccagaaca gagactgaac ttgaaaatat ccagaacaat atctggcaag agtatgaagt
    19141 cacctatgcc aacgccttgg gatacaggga caatcagttg gggcccgccc gggttaaaag
    19201 ggaaattcat gagattaagc agagaattga caggcttgga gatgtaaatg taaaggctat
    19261 agaagaatac aagcgaataa aagagcgttt tgatttcctt acccgccaaa aggatgatct
    19321 taccgaagcg aaggataatt tattgagcgt aataagcgac attacatcaa ctatggagca
    19381 aaggtttata aaggaatttg ccattatcaa cgaccacttt aacggtgttt ttaggcgttt
    19441 gtttggcggg ggcaaggccg gtcttgtatt ggaggacccg gatgatgtat tggcatcagg
    19501 aattgaaata attgctcaac ctccgggtaa gaaactccaa aatatcaatc tcttgtcagg
    19561 cggcgaaaaa tctttaacgg caattgcaat cctttttgca atactagagc ataaacctgc
    19621 acctttttgt gtactggatg aaatagaagc cgcgctggac gaaagcaatg tccaaaactt
    19681 tgggaaattc ttaaaagaat tttgtgaaag aacccaattt gtattgataa cccacaggaa
    19741 ggggactatg gagaacagcg atgtcctata tggggttgcc atggaagaaa aaggggtctc
    19801 cagaatgata tccgttaggt tggaagatga aattgcgagt taggacaaaa agcagatgga
    19861 aaagggaggc aacataaaat cgggatgaat aaggaaaatg ataagaaaac aggcttcttt
    19921 tcaaggttaa taaagggttt ggataaggct aggcagggat ttaccaccag ggtggatgag
    19981 cttatcaagt attatcgtga aatagacgat ggcttttttg atgaactgga ggagatcctt
    20041 ataactgcgg atgtaggcgt taatacaacg gtggaaatcg tagacgagtt acgggatctg
    20101 gtcaaagaaa aaaagatcgg agatgccgaa aggataagaa atctgctaaa ggaaaagatt
    20161 gctgccatct tgaccggcac ggaagagttc gaagtaaccc ctcccgcggt ctgtctggta
    20221 gtaggtgtta acggggtcgg caagaccact accatcggaa agctgtccgg tctttataaa
    20281 aggcaaggca gaagcgtttt gatggctgca ggcgatacct ttagggcagc ggctacggat
    20341 cagttgcaaa tttgggggga cagggccgga gtacaggtag tttcccaaga tgagggttcc
    20401 gatccggccg ctgtgatctt tgatgctgtt caagcggctc gttccagaca tatcgatgtc
    20461 ctattgtgtg ataccgcagg caggcttcat agcaaaaaga acctgatgaa cgaattgggt
    20521 aaaatcaatc gaatcatcga tagggaatac ccggaggcgc aaaaagaggt atttttggtg
    20581 ttggatgcga caaccggtca aaacgccata acacaggcta gactttttca agaggtagta
    20641 ggcattaccg gaatcattct gacgaagctg gacggtacgg ctaagggcgg cattatcgtg
    20701 gctataagaa gcgaattgga tatccccatt cggttcatag gagtagggga gcagatagat
    20761 gaccttcaac ccttcgtccc ccaagacttt gtggacgcac tgtttggcta attgcatatt
    20821 ttgggtaggc cccgcagcag gtttactcgt tcttgccttg gagtctagat gattggaata
    20881 ctattcttta agttaagatt tacgcactct ataattttag attattgttg acaggtgagc
    20941 tggcatatac tataatcatt acctgtaaag ggatactact ttacagaagc ggatccactt
    21001 tctatatgtt ttacgataaa ctttctgcat tggtataaag atatcgtaaa acattatcct
    21061 taattcgtat gtggcggaaa acattatttt aacgtaaaat tagataattt aaccaatttt
    21121 acaacgaaag gtctttttcg caacatatat aggagtgtgg gaatggaaaa gtttactcat
    21181 ttagggatgt tgtttgagat ttatggcggg ctgttaacac ataggcaaag ggatgtgatg
    21241 gatttatatt ataatcatga cctctccctt gccgaaatag ccgagcagtt aagtattagc
    21301 cggcagggtg tacatgatct tgtaaagagg tcgggtctat cccttgaaaa tgcggaaaat
    21361 aacattggat ttttaaagtt ccggggcggg attttccatg ggataaacga tatcgaaagg
    21421 gatctagaca ggttgctcag ttgtttgggt caaggggctg aaggcgaagg gatcgaaaaa
    21481 gaaccccatg agctaaaaag aaaattaagc cttattaaag ataagattat gcgaataggg
    21541 gatttatagg agtccagttt tgaaatggtg acggctataa tctgaggagg tttatgaatg
    21601 gcatttgaag gattgtcgag taaattgcaa tcgacattta aaaaattgac gggtaagggt
    21661 aaactgtccg aaaaagatgt aaaagatgcc atgcgggaag tgagattagc acttttagaa
    21721 gccgatgtca acttcaaagt agtgagagat ttggttaaaa ggattgggga ccgggccgta
    21781 ggccaggaga taatgaggag cctgaccccg ggacaacagg ttattaagat tgtcaatgag
    21841 gaactaactg atttaatggg cgggacctat acaagaatag caactgccga taagccgcct
    21901 actataataa tgatggttgg cctgcagggg gccggaaaaa ccacaacagc tggtaagctg
    21961 ggtgggtatt tcaagaagga agggaagaac ccccttcttg ttgcatgcga tatatatcgc
    22021 cctgctgcaa ttaagcagtt acaggtagta ggggatcagc agtcgatccc ggtgtttgag
    22081 atgggtaagg atgaccctgt tcgcattgcg tcaaaagctg ttgcccacgc aaaggatcag
    22141 gggtatgata ttgtaattgt tgatacggcc gggcggctcc atataaatga ggaattgatg
    22201 aacgaattgt ccaatatcca ggaggctgtc aagccacaag aaattttact ggttgtcgat
    22261 gccatgaccg ggcaggatgc cgtcaacgtt gcccaagact tcgacgagag gttggaaata
    22321 gatggggttg tgcttaccaa actggacgga gattccagag gtggggcggc cctctcggta
    22381 agggctgtta ccggtaaacc tatcaagttt gccggcatgg gtgagaagct gaccgatttg
    22441 gaattatttc atcccgacag aatggcttcc agaatccttg gcatgggtga tgttttaagc
    22501 ctgattgaaa aagcccaggc aaatatcgat accgagaagg ctttggaact tgaaagaaag
    22561 cttaggacac agcaatttac tttcgacgat tttctggagc agcttcagca actcaaaaac
    22621 atgggcccct taagcgatat agtcggcatg attcccggaa tgaaccccgg caagctgggc
    22681 ggtgttaatt tagacgacag gcaactagta agaaccgaag ccattatcca gtctatgacc
    22741 aaggaagaaa gatttaaccc cggcattata aatgccagca gaaggaaaag aattgccagg
    22801 ggcagcggaa ccaatataca ggatataaac aaactgttaa aatcctttac agaatttcga
    22861 aagatgatga agaggatgtc cgggatggag aaatccatga agagggggaa gtttaagttt
    22921 ccgttctaaa ttgcagccgg gattgttaat tgtattttac ggttaaggat gccggcaaaa
    22981 atcatgggct tgcaggttaa agaaaattac cctttttcgg tgcagttgtt ccgatgcttt
    23041 gtttcagttg attatagaga tatggactgc tgcccgatta atagggttaa tatattgatt
    23101 at
//
LOCUS       DASRNX010000054         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_329116,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000054 DASRNX010000000
VERSION     DASRNX010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329116"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFD89_02210"
     CDS             <1..720
                     /locus_tag="VFD89_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ57035.1"
                     /translation="TDFEAAMENVGGIIENPDLYKRLTGRQNLEYFASMYKNVNKENI
                     DNIVDMVKMSGRINDKVKTYSLGMSQRVGIAQALLHNPRLLVLDEPTNGLDPLGIKEL
                     RDLLKYLAKEAGVGVFISSHLLSEMELMCDRIYIIDEGVIIGEKTIHEEEERVRDDIY
                     EYTFLTDDRDKTLEYLNSLQANCRVENGNVIVIMKRSEMPNIIKNLVYRDIGIYAASE
                     KHRTLEQDFISITTGTKTQIR"
     gene            752..2569
                     /locus_tag="VFD89_02215"
     CDS             752..2569
                     /locus_tag="VFD89_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZJ57036.1"
                     /translation="MLFLRQVRFEIKNIIRSKFILIIGILILAICIATPIISLIGQRS
                     SKGGGMYYGYDMGMGGMGMVRSVAYDMPWYPGPGGYEEEPIIVDGITIKSDNPFFWNV
                     KSLIDERGYMENDKGNFSDPKVLDLILSTMDVEQEYFVYLAEHITDHRDYRTEISWVG
                     RESIYDKFVYEHTNLPKEQLYEAMSYRMGYDPEMFDEKFINITPEQRLAAIDEIDEQL
                     NLLYKVVETGDFPLFIDLRIKQEMKSIEDTKERIAVQEKIIIDDPSQEEWVSQYIVDM
                     NNEIDLIETNNIPTLEYRLENNIIPGEDIWQNTALSNIEMNKRNLMYNKILTEEEFNR
                     ERYLVDEYKTYRRYESIMQEERDGYNNQIIIAERSLDAGKPDMNFEPWGARSRTFSFL
                     SYSSFVAMFAILIGGWLMASEFQQGTIRLLMIRPKTRTKILMAKFLSAFIIFLGLYAL
                     GSLLNMITNGACFGFKDFLYPNFTISGEISFFAYYLPKFVVCIMPAIFTFSLSFLLSV
                     LIRNIAVAIIIPIALFIGSTILMQILVYGNTIAKWLAFTPIPYMDFTNFFSQHSIVNN
                     AIQKGIPLGMGYGILMLLGLSTVFVILSIFTFKRRDITN"
BASE COUNT          840 a          523 c          651 g          709 t
ORIGIN      
        1 acggattttg aggctgccat ggaaaatgtg ggggggatca tagaaaatcc ggacctatat
       61 aagaggctta cgggcaggca aaacttggag tactttgcat ccatgtacaa aaatgtgaac
      121 aaggagaaca tcgataatat agtcgacatg gtaaagatgt ccggcagaat taatgacaag
      181 gttaagacat attccctggg tatgagccag cgggtgggaa tagcccaggc actgctgcat
      241 aatcctcgcc tcttggtact ggatgagccc accaacggac tagacccctt gggtattaaa
      301 gagctgaggg atctcttaaa ataccttgca aaagaggcag gggtgggagt atttatatca
      361 agccatctgc tatccgagat ggagcttatg tgcgacagga tttacataat agacgaaggg
      421 gtaattatcg gggaaaagac catccatgag gaagaggaaa gggtcaggga cgatatatac
      481 gaatatacct tcttaacaga tgatagggac aagaccttgg aatatctaaa tagcctgcaa
      541 gcgaattgta gggtggaaaa cggcaacgtc atcgttatca tgaagcggag tgaaatgcca
      601 aacatcataa aaaatcttgt gtatcgggat ataggcatat atgctgccag tgaaaagcat
      661 agaacccttg agcaggattt catctccatc actaccggaa cgaaaaccca gatcagataa
      721 tttccaattt aatttaaagg gggatatgtt gatgcttttt ttaagacaag tcagatttga
      781 aatcaaaaat ataataagat ccaagtttat attgattatc ggtattttaa tactggccat
      841 atgtatagcc acgcctataa tcagcctaat cggtcaaaga tccagcaagg gaggggggat
      901 gtactatggc tatgacatgg gtatgggggg catgggaatg gttaggtccg ttgcctacga
      961 tatgccttgg tatccgggtc ccggcggata cgaggaggag cccataatcg tggatggcat
     1021 aaccatcaaa tcagataatc ccttcttttg gaatgtaaag agccttattg atgagcgggg
     1081 gtatatggaa aacgataagg gcaatttttc cgaccctaag gtattagacc taatactctc
     1141 caccatggat gtcgaacaag agtattttgt atacttggca gagcatataa cggaccatag
     1201 ggattaccgc actgaaatat cttgggtcgg cagggagagt atctatgata aattcgtata
     1261 tgagcatacc aacctcccca aagagcagct ctatgaggcc atgtcctata ggatgggcta
     1321 tgatcctgag atgtttgatg agaagtttat aaatatcact cccgaacaaa ggctggcagc
     1381 catagacgag attgacgagc agctgaacct actttataaa gtagtggaaa ccggcgattt
     1441 tcccttgttc attgacctgc gtatcaagca ggaaatgaag agtatagagg atactaaaga
     1501 gagaatagcc gtacaggaaa agatcataat cgacgatccg tcccaggagg aatgggtaag
     1561 ccagtatata gtggatatga acaatgaaat tgatctcatt gagaccaaca acattcccac
     1621 cttagaatac agactcgaaa ataatatcat tccgggtgag gacatttggc aaaacactgc
     1681 cctttccaat atagagatga acaaacgaaa tcttatgtac aacaaaattc tgacggaaga
     1741 agaatttaac agggaaagat atctggtcga tgaatataag acgtacaggc gatatgagag
     1801 catcatgcag gaagaacggg acggttacaa caaccagata ataattgcag agcgttcatt
     1861 ggatgcaggc aaaccggata tgaatttcga gccttggggc gcaaggagca ggacctttag
     1921 ctttttaagc tattcatctt ttgtagccat gtttgccatt cttatagggg gctggttaat
     1981 ggccagcgaa tttcaacagg ggaccatcag gctgttgatg atccgcccga aaacccgtac
     2041 taagatattg atggccaagt ttctatccgc ttttattatc ttcttgggcc tgtatgcctt
     2101 ggggagcctt ctcaatatga tcaccaacgg tgcctgcttt gggtttaagg attttctata
     2161 ccccaacttc accatatccg gggagataag cttctttgcc tattatctgc cgaagtttgt
     2221 agtctgcatt atgccggcaa tctttacatt ctccctatcg ttcttgttgt cggtactcat
     2281 cagaaatatt gcagttgcca tcatcatccc catagcgtta tttatcggct ctactatatt
     2341 gatgcagatt ttggtctatg gtaacaccat agccaagtgg cttgccttta caccgattcc
     2401 ctatatggac tttactaatt tcttttccca gcattccata gtaaataatg ccatccaaaa
     2461 gggtatcccc ttgggcatgg ggtatggtat actgatgtta ctgggtctgt ctactgtatt
     2521 tgttatactt tctatattca ctttcaagag aagggatata actaactaga gagggtacgg
     2581 ggtaatttca atggatgcaa agaataaatt tttagaatcc tataagaaga aaacagagga
     2641 aaagggcggc gaaggccaaa ttaacgatcc acctgcagac atagccggcg gagataatac
     2701 gagcgtaccg tccggggatg agg
//
LOCUS       DASRNX010000055        17753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_62630,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000055 DASRNX010000000
VERSION     DASRNX010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17753
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62630"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     misc_binding    193..472
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            728..1945
                     /gene="tyrS"
                     /locus_tag="VFD89_02220"
     CDS             728..1945
                     /gene="tyrS"
                     /locus_tag="VFD89_02220"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZJ57037.1"
                     /translation="MVAIDEQIKIISKGADEIIDIGDLKAKLTKSQKEGKPLTVKLGL
                     DPTAPDIHLGHAVVLRKIKQLQDLGHRAIIIIGDFTGMIGDPSGRSKTRPALTKEEVL
                     KNARTYEEQIFKVIDRDKTEVRFNSEWLAPLNFSDVIVLASKHTVARMLEREDFKKRF
                     EGHESIGIHEFFYPLMQGYDSVAIKADIELGGTDQRFNLLMGRTIQRDFSQESQIALM
                     MPLLEGTDGVEKMSKSLGNYIGIYEEANEMYGKVMSIPDELMIKYFELATDIHPDEIS
                     VIKAGLEDGSYHPRDAKMRLAREITALYHSEDAAQKAQEQFKAIFQRGGMPDEIPTIE
                     IPEDIISQEGVDMARLIVAAGFSPSTSQARRLINQGAVRINGEKRDELKSTSLKDQDV
                     VQVGKRNFIKVIM"
     gene            2063..2749
                     /locus_tag="VFD89_02225"
     CDS             2063..2749
                     /locus_tag="VFD89_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HZJ57038.1"
                     /translation="MFKELKEEVWKANMDLPKYGLITFTWGNVSGIDREKGIIVIKPS
                     GVPYSELEPRHMVVLDMDGNKVEGDLRPSSDTPTHLVLYNSFMEIGGVVHTHSPWATS
                     FAQAGRGIMALGTTHGDYFYGEIPCTRKMTAEEVQGEYEVETGNVIVETFIGVSPMDI
                     PGVLVNMHGPFTWGKDASEAVHNAVVMEEVAKMAYRTHTLNDNIGPMDKTLLDKHFLR
                     KHGKDAYYGQ"
     regulatory      2838..3004
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            3337..4674
                     /gene="mgtE"
                     /locus_tag="VFD89_02230"
     CDS             3337..4674
                     /gene="mgtE"
                     /locus_tag="VFD89_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZJ57039.1"
                     /translation="MTEEFILGLIHQGRLAEARTWITQKNVVDIASWLGDLDENNVLV
                     VFRLLPKDLAADVFSYVSNEQQGFIIESITDREIKNIIDELFLDDTVDLIEEMPANVV
                     KKVLKNTGEERRQLINQFLKYPENSAGSLMTIEFVDLKKEMSIDQALSYIRRVGLDRE
                     TINICYVMDENRKLEGIIPIRKLILSNPSETIKNIMETNIISVHTLEDQEEIADIFKK
                     YDLIAVPVVDNEGRLVGIITIDDIVDIIEQENTEDFQKMAAMHPSDEEYLKTGVMALA
                     RHRLPWLLILMISATFTGGIIQRFEDVLQSVIILNSYIPMLMDTGGNSGSQSATLIIR
                     GLALGEIGLNDSLKVVWKELRVSILVGIALALTNFARILILGQASVWIAATVSVSLIV
                     TVVLSKVIGGALPILAKKMRMDPAIMAGPLITTIVDALALMAYFSMASWILGI"
     gene            4815..6488
                     /gene="araB"
                     /locus_tag="VFD89_02235"
     CDS             4815..6488
                     /gene="araB"
                     /locus_tag="VFD89_02235"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HZJ57040.1"
                     /translation="MNKKYAIGVDFGTQSGRAVLVEIGTGAEIATAIKEYPHGVMDEY
                     LPDGVTRLEPDFALKHPQDYLDVLGETIPAVLEESGISARDVIGVGIDFTACTMLPID
                     ENGTPLCMYDEYKTNPHAYIKLWKHHAAQDEANKLNEIATERGEDFLARYGGKISSEW
                     LIPKIWQILDEAPEIYERSHRFIEAADWIIMQLTGQEKRNSCTAGYKGLWHKKKGYPE
                     SEFFKALDPRLENVVDEKLSRDIYPIGSKAGEITEKAARLTGLNPGTAVAVANVDAHV
                     SMPAVGIVDEGKMLMIMGTSTCHLILGKEEKMVPGMCGVVEDGIMPGYFGYEAGQSCV
                     GDHFDWFINNCVPGDYYKEADDKGINIHDLLSDKAGELAVGESGLVALDWWNGNRSVL
                     VDVDLTGLIIGCTLLTKPEEIYRALIEATAYGTRMIIDTFEESGVPIHELFAAGGIAD
                     KNPFLMQVYSDVTNREIRISASAQAPALGSAMFGAVAAGRDRGGYDSIVDAASEMARI
                     KDVVYRPIPTNVEAYNKLYEEYKVLHDYFGRGANDIMKRLKNLRRDTTN"
     gene            6717..7808
                     /locus_tag="VFD89_02240"
     CDS             6717..7808
                     /locus_tag="VFD89_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HZJ57041.1"
                     /translation="MKEKFNGLGMNMGNLWRLSDARTRSISPENFNGAKGKGGMATLE
                     EGAARKAARELGQGWKVNPYIIIEPGETFTIADIEGPGAIQHIWMTPTGSWRNTIMRI
                     YWDGLEHPSVECPVGDFFACGWGEYAQVSSLAVCVNPGSAFNCYWEMPFKKNAKITME
                     NRDSEPMVLYYQIDYTLTEVSEDVGYFHAQFRRTNPLPYKDVYTILDHVEGKGQYVGT
                     YMAWGVNNSGWWGEGEIKFYMDGDEEFPTICGTGTEDYFCGSYNFENKNTKQYQEYTT
                     PYAGLPQVIRPDGVYRSQTRFGMYRWHISDPIRFESDLRVTIQALGWRKDGRYLPLKD
                     DIASVAYWYQTLPGRKFPQLPDRDYLEII"
     gene            8174..10240
                     /locus_tag="VFD89_02245"
     CDS             8174..10240
                     /locus_tag="VFD89_02245"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZJ57042.1"
                     /translation="MKLGLGIDTGGTYTDGVIYDFNSSKVIKGAKSITIKEDLKLGIM
                     DVLDQMPKDLLNRVGMVSLSTTLATNACVEDKGSRARLILMGCDQDVVNKNGAQYGLP
                     PIDDIIFIDGGHDSRGEVVAEPDWDKLIKEVERTRGGVDAFAIVQMWGIRNPEFEQKA
                     KTLIKESTGLSVVCGHELSSQLNFLKRGATALLNARLIPLVDEFIDAVKVALEERGIA
                     APLVIVCGDGSIMSEEFVRDRPVETLLSGPAASVIGGVNLSGKQNCVVVDMGGTTSDL
                     AIIKDGRPLLAQEGAKVGSFNTAVKSVDIRTMGMGGDSLISFDSGDSLTIGPRRAAPL
                     SWLAWKWPDVLGQISLLHEENRIHTRSLCQFFYKVWDIPGDSDFTPEERAIVEALSDS
                     PLSIERLAEAVDSSIYTIKLHRLEELGIIMRSGLTPTDIMHITGDFTGWEKNSAEMGA
                     KILAMRLGMNLDELIHEVNTMIKSQLYLSIAKELMGRGNPGISPAIPHETMTPEMEKL
                     ILLGFKDPRKKSSPYISLELATGFSLVGIGAPIHVYLPDVAASLDTQCIIGEHAPIAN
                     AVGAITGSISAEESVIIRPRYIMTGVELYDCFSSGEKRQFNNYEKAKEWALAEARTLA
                     SDLVKAKGAAYWDISVELCENNTEINAYSPGILGEKDQEQSEITREILLETTVTARGI
                     GGLKWA"
     gene            10231..12108
                     /locus_tag="VFD89_02250"
     CDS             10231..12108
                     /locus_tag="VFD89_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HZJ57043.1"
                     /translation="MGIGKKGGVMMDVNKCTITVIHQGNKRTVTCPKGSNLMGVFHRK
                     NIHVEAPCGGKGICGKCKVMVLKGPVSEIGHGESRFLSNNERARGYRLACMTKVFGDI
                     DVVIDNHLDGAQIMSTGMEYSVDISPRIDKKFVQLDLPTVDDQRDDLKRLADSIGLRA
                     PGIRIGLIRKLPDLLRDSDYKVTAAYDNNYLINVEKGDTTASMYGVSIDIGTTTVVCY
                     LMDLKTGRLVDTISELNVQRIYGGDVISRIQHSMENEDGLARLRWGIVDQITGLINEL
                     ADRNHIIMDHIYNVVIAGNTIMGHLFMGIDPQHIASTPFTPALTQQAVYPAHELGLKL
                     GVSARIFMLPHISGYVGADVTAGILASGIDKEDDLSLIIDIGTNGEIVLGSSKKMVCC
                     STAAGPAFEGANIRHGMGGIRGAINTIRLNDGRLKYTTIGDEAPVGICGSGIVDALAF
                     LLDTGLVDETGRLLDEDEIESETGKSLAHRFTEIDGQGAFVLVDAKSSSTGEPIVITQ
                     KDIREIQLAKAAISAGIKVLIHRMGKGMGDVTRLYLAGGFGSYIDKGNAAKIGLIPRE
                     LQNRIIAIGNGAGTGAMLSLLSAEQFKRADLIGNMAEYVELSSTPEFQDEYVNGMYFT
                     T"
     gene            12287..13600
                     /locus_tag="VFD89_02255"
     CDS             12287..13600
                     /locus_tag="VFD89_02255"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HZJ57044.1"
                     /translation="MAFWRSRGEIGDDVGLDMDLAIEAIGHYNRVAGVGISIIDTRGK
                     PLYNIGYTGLCCKFCSGVRDILDTEDDYCDRVHLYGGYQSERFGGRYIFFCPLGLVHW
                     ASPVSSEGMVRGYLLCGPALMVAPEEFLIEDLLLKNRIDKEHLGRLKENLEHIPLIPP
                     DRVTSLSELLFMVASHLSDAEYFQYLSQAEFHNHQSNISEYIHSIKAVAGSQGDGADY
                     PFEKEKELLSCIVAGDKKGSQTILNRILGHIFFSSGGDFETIKARILELIVLLSRAAV
                     EGGADVEEIFGLNFKYLSTIHSFKTVEELTYWLSKIMIRFTDCVFNLKNIKHVDVIYK
                     AINYIKQNYMKKITLEEVASHVYLSPSYFSKIFKDDMKCNFNTYVNRVRIERSKQLLL
                     QDTINLADISNMVGYEDQSYFSKVFKRVVGITPGRYRLGRGKLKF"
     gene            13612..14250
                     /locus_tag="VFD89_02260"
     CDS             13612..14250
                     /locus_tag="VFD89_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HZJ57045.1"
                     /translation="MALLEEISKYLQQGRAKNVKELVQKAVDEDLEPREILNEGLLDG
                     MGIIGEKFKNNEVYVPEVLIAARAMYAGMEILKPILTETGVESIGKVVIGTVKGDLHD
                     IGKNLVKMMMEGRGLEVIDLGIDVSPERFVEAAKENDAQIIACSALLTTTMTEMKNVV
                     EAAKESGIRDQVTIMVGGAPVTDNFCKSIGADIYTADAASAAEEAARVCKSA"
     gene            14622..16772
                     /locus_tag="VFD89_02265"
     CDS             14622..16772
                     /locus_tag="VFD89_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HZJ57046.1"
                     /translation="MEYLNGVVERITYYNEDSGYTVIRLRSRGYYDLVTVVGNMASVH
                     VGAIVSLRGSWKVDSKYGKQFIAEFYEEKLPASVAGIEKYLGSGLIKGIGPINAKRIV
                     KAFGKDTIDVIEEEPDRLMEVPGIGPKRIDMIKKAWQEQREVKNIMLFLQSHGVSTSH
                     GAKIYKTYGNESLNVVKENPFRLADDIWGIGFRTADTIAQKLGFDPQSMQRCRSGILY
                     ILNQLSNEGHCFATRDQLIMETKETLDLDDAKIQESIDIMVGDGSLIYDQEALYLPPF
                     FHSEVGVARRIREIASTPASLDIVGIDEIIRGIEDRGRIKYDQIQQEAIKRSASSKLM
                     VLTGGPGTGKTFTILGIIRVFERIGAKIYLAAPTGRAAKRMEEATGREAKTIHRMLEY
                     KPPQGYMRNVENNPLECDVLIIDETSMVDIILMYNLLKAVRDETIVILVGDVDQLPSV
                     GAGNVLSDIIASGEVDVVKLTRIFRQAQNSAIITNAHRINQGIFPMLKGGKNRDFFFI
                     RREDPEEIAGEIRRLCRVRLPKYYKVDPIDDIQVLCPMQRGETGAVNMNRILQEALNN
                     NSHFIRYGGATYKVGDKIMQIRNNYDKNVFNGDIGRIFSIDLEDKIVNIDFDGSIVEY
                     DSSELDEIMLAYAVTVHKSQGSEYKIVVAPFTTQHFMMLQRNLLYTCITRARNIFIMV
                     GTEKAVGIAVGNNRIVKRNTMLDKRLKDPRLDRR"
     gene            complement(16795..17184)
                     /locus_tag="VFD89_02270"
     CDS             complement(16795..17184)
                     /locus_tag="VFD89_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZJ57047.1"
                     /translation="MAIDKEMSSGDYGKTVIANEVVAVIAGLATAEIPGVASMSSGLV
                     DDITSTLGMKSSKRGVKVELIDEIATIGISIVVNYGVRIPEVAENIRSAVIEAVQGMT
                     GLTVDEVNIYVQGVYLEKDAREPVMEV"
BASE COUNT         5272 a         3451 c         4624 g         4406 t
ORIGIN      
        1 catcctatat gcttcttata aaggcgctga actaatctat agttggttgc actatataaa
       61 tgcatcttta ttatgagggc agatagttac tgaaaagcat acaaataatt ttcttgattt
      121 taacggggga aaaaatcaag gatttattga cattcccagg atttatagta tataatacag
      181 taaatttcac atataaaagc gatgatgaga agaagtagaa gtgcttaaaa accttaaaga
      241 gagttcccgg taggtgagag gggcaaggaa agcgcacttt gaatacatct ttgagctgtg
      301 caccgaaagg gcctaaggac cttagtaggc tgtcaacggg atttgcgccc gttatcgtgc
      361 aaaggtatac aagccatagc tttgatgttg atggactgta cctaatgagg tcgatgccgt
      421 gaggcgtgga tgaacttggg tggtaacgcg agatagaact ctcgtccttt ggattatacg
      481 aagggcgggg gttttttgat atttagggga aaactaggcg cgttacagga gcatttacag
      541 cattgtatta aagcgactac atgcttacga tatgtatttt aaattgatgc gttatgctgg
      601 gccattacct tactatgcga tgaaggtaat gagctattcc taagagtatg acttcattag
      661 ctgtgaaatt acgagcctat tatgtaacat atggagataa aaaatagata aaaacggagg
      721 aataaaaatg gtagccatcg acgaacagat caaaatcata tccaagggag cggatgagat
      781 aatcgatata ggggatttaa aagccaagct tacgaaatcc caaaaggagg gaaagccact
      841 tacagttaag ctgggccttg atcctaccgc ccccgatatt cacctaggtc acgccgtggt
      901 actgagaaag atcaaacaac ttcaagactt aggtcatagg gctataatta tcataggaga
      961 ttttaccgga atgatagggg acccctccgg caggtccaag accaggccgg cccttacaaa
     1021 ggaagaggtc ttaaagaacg ctcgcaccta cgaggagcag atatttaagg tcatcgacag
     1081 ggacaagacc gaggttcgct tcaacagcga atggctggca ccccttaact tttccgatgt
     1141 catagtttta gcctctaagc ataccgttgc caggatgctg gagcgggagg actttaaaaa
     1201 acgttttgag ggtcatgaga gcataggcat tcatgagttt ttctatccct tgatgcaggg
     1261 atatgattcc gtagcaataa aggcggatat tgaacttgga ggcaccgatc agaggtttaa
     1321 tctacttatg ggaagaacca ttcaaaggga ttttagccag gagagtcaaa ttgcccttat
     1381 gatgccgcta ttggagggta ccgacggggt agaaaagatg agtaagagcc tagggaacta
     1441 catcggtatc tacgaagagg ccaatgagat gtacggtaag gtcatgtcca tacccgatga
     1501 gttgatgatc aaatactttg agctggctac ggacattcat cccgatgaaa taagcgttat
     1561 aaaagcagga ctggaagatg gctcatatca cccaagggat gccaagatga gactggccag
     1621 ggagatcact gccctatatc atagcgagga tgcagcccaa aaagcccagg aacaattcaa
     1681 ggctatcttc caaagaggtg gaatgcctga cgaaattcca acaattgaaa tccccgaaga
     1741 tatcatatct caagagggcg tggatatggc ccgcttaatc gttgcggccg gattttcacc
     1801 cagcaccagc caggccagga ggcttatcaa tcaaggcgcc gtaaggataa acggggagaa
     1861 gagagacgaa ctaaaaagca catccttaaa ggatcaggat gtggtacagg taggtaaaag
     1921 aaactttatt aaggttatta tgtaggtaat actgacgggg atatgggctg gatgcctcaa
     1981 aacgtaatca gcatatttta gggctaatcg gcacaaagat tactgataca ggaaattatt
     2041 gacgcatggg ggggatgatc cattgttcaa agaattaaag gaagaagtat ggaaggcaaa
     2101 tatggacctg cccaagtacg ggcttataac atttacttgg ggcaatgtca gcggtatcga
     2161 tagggagaag ggaattattg ttataaagcc cagcggtgtt ccctattcgg agctggagcc
     2221 ccggcacatg gtggtattgg atatggatgg gaataaggta gaaggagacc tcagaccttc
     2281 ctccgatacc ccgacccatc tggttcttta taacagtttt atggaaatag gaggggttgt
     2341 tcatacccat tctccttggg ctacaagctt tgcccaggcg ggcaggggga taatggctct
     2401 tgggactacc catggggatt atttttatgg cgagattcct tgcaccagga agatgaccgc
     2461 agaggaggta cagggagaat acgaagtgga gaccggaaac gtcatagttg agacctttat
     2521 cggagtaagt cccatggata taccgggtgt tttggtcaat atgcatggcc cctttacctg
     2581 gggtaaggat gccagcgaag cggtccataa cgccgttgta atggaggagg tggccaagat
     2641 ggcctacaga acccatacct taaatgataa cattggcccc atggataaga cactgcttga
     2701 taagcatttt ttaagaaagc atggtaaaga tgcgtactat ggacagtaga gggcattcaa
     2761 ttatattccc ttaatccatc ggtggttgaa tttatgggaa gcatggtata taatgtattc
     2821 aggtacaaat tcaaataaca aattttcgtt aggtgaggct actatacaga aacacgccac
     2881 tatccagaaa cgtcgagaga cgccaacggg tcaacaggct ttatcggagc aaggtatagc
     2941 ctaatgtggc tggtttatat aacctaagct gtatagtgct aaaactcaac gagtggaagc
     3001 gtcggtagat atctttgggt ctttcgcttg ttgagggacc catttttatt accaaaaaga
     3061 acggaatctc tatggttggt tgcttctatt gtaaggcatt ttaaaggggc ggaaaaattt
     3121 ctcccatttc tataagtggt ggactccgac tagttaaata ggcagctgat acaaataccc
     3181 catagggatt ttgttgaata ttataaaaag gatggtgacg gaaagtggag cctgggcctt
     3241 tgcctagttt atgccaatgg gaaaaagtta ataatatgga cagggtctcg gccaccagat
     3301 tgtaaatggc agagccctgt tgaggagggt tatgccatga cagaagaatt tattctaggc
     3361 ctcattcacc aagggaggct agccgaagcc aggacctgga ttacccaaaa aaacgttgta
     3421 gacatcgcaa gctggttggg ggatttggat gagaacaatg ttttggtggt atttagatta
     3481 ttgcccaagg atctggctgc tgatgtattt tcttatgtat ccaatgagca acagggattt
     3541 atcatcgaat ccataacgga cagggaaatt aaaaacatta tagacgaact atttttagac
     3601 gatacagtgg atctaattga ggagatgcct gccaatgtag tcaagaaggt gctcaaaaac
     3661 accggcgagg aacggagaca gcttataaac caattcttaa aatatccgga aaattcagca
     3721 ggcagcctga tgaccatcga atttgtggac ttaaaaaagg aaatgagtat agatcaggcc
     3781 ctatcctata taaggagggt cggccttgat agggaaacca taaacatttg ctatgtaatg
     3841 gacgagaaca gaaagctaga gggaatcatt cccattagaa agctcatatt aagtaatcct
     3901 agcgagacca taaagaacat aatggagacc aatattattt cggtgcatac cctagaagac
     3961 caggaagaga tagcagatat atttaaaaaa tatgatctga tagcggtccc ggtggtggat
     4021 aatgagggca ggctggtggg tatcattacc atcgatgata ttgtcgacat tatagagcag
     4081 gaaaataccg aggactttca aaagatggcg gcgatgcatc cgtccgatga ggaataccta
     4141 aagaccggcg ttatggcgct ggccagacat cggttgcctt ggctactgat attgatgata
     4201 tctgctacct ttaccggggg tataatccaa agattcgagg atgttttgca atcagtaatt
     4261 atactcaatt catacatccc catgctaatg gatacgggag gcaattccgg ttcccaatcg
     4321 gccaccctta ttattcgtgg cttggccttg ggggagattg gccttaacga ttccctaaag
     4381 gttgtatgga aagaattgag ggtaagcatt ttggtgggaa tagcgctggc tctcacaaac
     4441 tttgccagga tccttatact ggggcaggcg tcggtttgga ttgctgcaac tgtatccgtc
     4501 agcttgattg ttactgttgt tttgtccaag gttataggtg gagcgcttcc catattggca
     4561 aagaagatgc gcatggatcc ggcaataatg gcgggacccc ttattaccac catagtagat
     4621 gctctggccc tgatggccta cttttccatg gcgtcttgga tattaggcat ttaaaagcat
     4681 aggaaaatcc taaaactata cgggggtggt ttctatgcca cccccgaaaa cgaattattc
     4741 actatttcaa ccgtataaca aaatcatgga caggatctta aaagaattat ttttagtaaa
     4801 aggaggccga tgggatgaac aagaaatatg cgattggggt ggactttgga acccagtcag
     4861 gaagggccgt attggtagag atagggacgg gtgcggagat agcaacggcc atcaaggaat
     4921 acccccatgg ggtgatggac gagtacctgc cggacggcgt tacccgcctg gaaccggact
     4981 ttgcattaaa gcatccccag gactacttgg atgtcttggg cgaaaccata cctgccgtct
     5041 tggaggaatc gggcatttct gcccgcgatg taatcggggt aggaatcgac tttacggcgt
     5101 gcaccatgct tcccatagac gagaacggaa cacctctttg tatgtacgac gaatataaga
     5161 ccaatcccca tgcctatata aagctgtgga agcatcacgc cgcccaggat gaggccaata
     5221 agttaaatga aattgctacg gagcgggggg aggacttctt ggccagatat gggggtaaaa
     5281 tatcctcgga gtggcttatt ccgaagatct ggcagatatt agatgaggct ccggagatat
     5341 atgaaagatc ccataggttt atagaagctg ccgattggat aatcatgcag ctcaccggtc
     5401 aagagaagcg gaacagctgc acagcaggct ataaaggcct ctggcataag aagaagggct
     5461 atcctgagag cgaatttttt aaggctctgg atcccagact ggaaaacgtg gtagatgaga
     5521 agctcagcag ggacatatac cccataggat cgaaggccgg cgaaataacg gaaaaggcgg
     5581 cccggcttac cggattaaac cccggaacgg ctgtagccgt ggccaatgtg gacgcccatg
     5641 tatccatgcc cgcggtggga attgtcgatg aggggaaaat gctcatgatc atgggaacat
     5701 ctacatgcca tttaatactt ggcaaggaag aaaagatggt tcccgggatg tgcggggtgg
     5761 tagaggatgg tataatgccc ggttatttcg gatacgaagc aggccaatcc tgcgttgggg
     5821 atcattttga ctggtttata aacaattgtg ttcccgggga ttattataag gaagctgatg
     5881 acaagggtat caatatccac gatctattaa gtgataaagc aggagaatta gcggttgggg
     5941 agagcgggct tgtagccctt gattggtgga acggaaaccg atcggtactg gtggatgttg
     6001 atcttacggg acttattatt ggctgtaccc tgcttaccaa gcccgaggaa atatacaggg
     6061 ccctaattga agcaacggcc tacgggactc ggatgatcat agataccttt gaggagagcg
     6121 gcgttcccat ccacgagctg tttgctgcag gcggaatagc agacaagaac ccctttctta
     6181 tgcaggtata ttctgatgtg accaacaggg agattaggat ttccgcctcg gctcaggctc
     6241 cggccttagg ctctgctatg tttggcgccg tagctgccgg cagggatagg ggcggttatg
     6301 attccatagt tgatgccgca tcggagatgg ccaggataaa ggatgtggta tacaggccca
     6361 tccctaccaa cgtagaggca tataacaagc tatatgaaga atataaggtt ctccatgatt
     6421 acttcggaag gggcgcaaac gatataatga aacggcttaa aaatctccga agggatacga
     6481 ccaattaatc gattcaatta gtcgggatat cattgcctat aaaaaaggtg ggggtatccc
     6541 ttcatatatc aggtagtaag atgatgatat atagataacc aatataggga ccaaatctat
     6601 cagtatgaaa attgcaatct ttctatacaa tttcatggtt ataaatttac agttggaatt
     6661 ggttatctat agcgcagagg gtcaagatga ataaggattg ggagggatca gttattatga
     6721 aggagaaatt taacgggtta ggaatgaaca tggggaatct ttggcggctg tcagatgcca
     6781 ggaccagatc cataagtcct gaaaacttca acggcgccaa gggaaagggc ggtatggcga
     6841 ccctggagga gggggccgcc cgcaaggcgg ccagggaatt gggacagggg tggaaggtaa
     6901 acccatatat catcatcgag ccgggggaaa cctttaccat cgcagatatc gaaggcccgg
     6961 gtgctatcca gcatatatgg atgacaccga cgggaagctg gagaaatacc ataatgcgga
     7021 tatattggga tggtttagaa cacccatcgg tggaatgccc tgtcggcgat tttttcgctt
     7081 gtgggtgggg cgagtatgcc caggtttcct cacttgcagt atgcgttaac cctggtagcg
     7141 cctttaattg ctattgggag atgcctttta aaaaaaatgc caagattacc atggagaatc
     7201 gtgatagtga gcctatggtc ctctactatc agatcgatta taccctgaca gaggtgtcgg
     7261 aggatgtcgg ttattttcat gcccagttta gaagaaccaa tcctcttccg tataaggatg
     7321 tatataccat actggaccat gtcgaaggta agggtcaata tgtcggaaca tatatggctt
     7381 ggggggttaa taacagcgga tggtggggcg agggagaaat aaagttctat atggatgggg
     7441 atgaggaatt ccctaccata tgcgggacag ggaccgagga ttatttctgt gggtcctata
     7501 actttgaaaa taagaataca aaacaatatc aagagtatac cacgccctat gcgggattgc
     7561 cccaggtaat aaggcccgat ggggtataca ggtcccagac caggttcggt atgtacaggt
     7621 ggcatataag cgatcctatc aggttcgaat cggatcttag ggtgactatc caggccctag
     7681 gctggcgcaa ggacggaaga tacctgccgc tcaaagacga tattgcctca gtagcctatt
     7741 ggtatcagac cctgcccggc aggaaatttc cacagcttcc ggatcgtgat tatttggaga
     7801 tcatctaata aacgagcgtt gcggataaaa acttgaggta agtaaagata gctgtccaga
     7861 aagaattctg tggcagctat ccttgtgagc aaattcataa ataacaataa actaatgtgg
     7921 tgtgctaatt catcaactca ctgtgtgatg aaggtaatga actactgaaa agcggacgac
     7981 ttcattgatt tggctcctgg aaattagaat ttggatatta gaagttagaa gcccgatacg
     8041 ataggctaaa taattacatt gacaattgcc cctcaatttt atatggtttt attgaatgtt
     8101 tttgtcgaat caaggtctaa acgaaaacag aacttgattc ctgtaatagt aacacattta
     8161 taggggtgac agcgtgaaat taggattggg aatagatacc gggggcacct ataccgacgg
     8221 tgtaatctat gattttaaca gtagcaaggt gataaagggc gccaaatcca ttactataaa
     8281 agaggattta aaattgggca ttatggatgt tttagatcag atgccgaagg atctgctaaa
     8341 tagggtgggt atggtgtctc tgtccactac ccttgcaacc aacgcctgtg tagaggataa
     8401 gggcagcagg gctagactca tcttaatggg ttgtgaccag gatgtggtga ataagaatgg
     8461 ggcccaatac ggtttgcccc ctatagatga cattatattt atagacggcg gtcatgactc
     8521 taggggtgag gttgtagcag agcccgattg ggataagctt atcaaagagg tagaaagaac
     8581 taggggagga gtcgatgcct ttgccatagt gcagatgtgg ggcattcgaa acccggaatt
     8641 tgaacagaag gccaagaccc ttataaagga gtctacgggg ctgtcggtgg tatgcggcca
     8701 tgagctatcg agccagctaa acttcttaaa gcgaggggct accgccctgt taaacgccag
     8761 attgattccc ttggttgatg aatttataga tgcggtaaag gtggccttag aagaaagggg
     8821 catagccgct ccgctggtca tagtatgtgg ggatggaagc ataatgtccg aagaatttgt
     8881 aagggacaga ccggtggaaa ccctgctttc cggtcccgcc gccagcgtta ttggcggggt
     8941 taacctaagc ggaaagcaaa attgtgtagt cgttgatatg ggcggtacca ccagcgatct
     9001 agccataatt aaagacggaa gacccctcct tgcccaagag ggggcaaagg tggggagctt
     9061 caataccgcc gtaaagtccg tggatatcag aaccatgggg atgggtgggg atagccttat
     9121 aagttttgat tctggagata gcctgactat aggacccagg agggctgcgc ctctgtcctg
     9181 gctggcatgg aaatggcccg atgtattggg tcagatcagc cttttgcacg aagaaaacag
     9241 aatccacact aggtccttat gccaattttt ctacaaggta tgggatatac ctggggatag
     9301 cgatttcacc ccggaggaaa gggccatagt agaggccctc agtgacagtc ccttaagcat
     9361 agagaggctt gccgaggctg tggactcttc catatacact ataaagcttc ataggctgga
     9421 ggagcttggc ataatcatga gaagcgggct gactcccacg gacatcatgc atattacagg
     9481 ggatttcacc ggctgggaaa agaattccgc cgagatgggt gccaaaattt tggctatgcg
     9541 tctgggaatg aacttggatg aactcatcca cgaggtcaat accatgataa agtcccaatt
     9601 atatctaagt atagctaagg agcttatggg aaggggtaat ccggggattt caccggcaat
     9661 tccccatgaa accatgaccc ctgaaatgga gaagctaatc cttttaggtt ttaaggatcc
     9721 ccgaaagaag agtagcccat atatatcatt ggaactagcc acaggatttt cattggtagg
     9781 gataggggcg cctattcacg tatatctgcc ggatgtggca gcctcactcg atacccaatg
     9841 catcattggg gaacatgccc caatagccaa tgccgtagga gccatcaccg ggagtatctc
     9901 tgccgaagag agcgtaataa taagacccag atatattatg acaggtgtgg aactctacga
     9961 ttgtttctct tcgggggaga aaaggcaatt taataattat gaaaaggcca aggaatgggc
    10021 ccttgcggag gctaggactt tggcatcgga tcttgtcaaa gcaaaggggg ctgcctattg
    10081 ggatatatcc gtagagcttt gcgaaaacaa tacggagatc aatgcttatt cacccggcat
    10141 cctaggggag aaggaccagg aacagtcgga aataactagg gagatattat tggagaccac
    10201 agtgacagcc agaggcatag gcggtttaaa atgggcatag ggaagaaagg cggggtaatg
    10261 atggatgtga ataagtgcac cataaccgtt atacatcagg gaaacaaaag gactgtgacc
    10321 tgtcctaaag gcagcaacct tatgggggta tttcacagga aaaacatcca tgtcgaagcg
    10381 ccctgtgggg gtaagggcat ctgcggaaaa tgcaaggtca tggtattaaa aggcccagtg
    10441 tcagagattg gccatgggga gtccaggttt ttatccaata atgaaagagc ccgggggtac
    10501 cgtttggcat gtatgaccaa agtattcggg gatatcgatg tagttataga caatcattta
    10561 gacggtgccc agattatgtc cacgggaatg gaatattctg tggatatctc acctcggatt
    10621 gataaaaagt ttgtccagtt ggatttacct actgtggatg atcagaggga tgatctaaaa
    10681 aggcttgcag attctatagg tctgcgggca ccgggtattc gaatcgggct tataaggaaa
    10741 ttacccgacc ttttaaggga ttcagattat aaggtgacgg ctgcctatga taataattat
    10801 ttgataaatg tggaaaaggg ggataccaca gcctctatgt atggtgtgtc catagatatt
    10861 gggactacca ccgtggtttg ctatctcatg gatcttaaaa ccgggcgctt ggtggataca
    10921 atttcggagc tcaacgtaca aaggatctat gggggggatg ttatctctag aatccagcat
    10981 tctatggaga atgaagacgg gttggccagg ctgagatggg ggattgtgga ccaaatcacg
    11041 gggctaatca atgaactagc ggataggaat catattatta tggatcatat ctacaatgtg
    11101 gttatagcgg gaaataccat tatggggcat ctttttatgg gaattgatcc ccagcatatc
    11161 gcatctaccc catttacacc ggctcttact cagcaggctg tctaccctgc ccatgaactg
    11221 ggtctgaaac tgggtgtatc cgctaggata tttatgctcc cccatatatc aggatatgta
    11281 ggtgcggacg tgacggcggg aattttggca tctggtattg ataaggaaga tgatttaagc
    11341 ctcatcatag atatagggac caacggggaa atcgtattag ggagcagcaa aaaaatggta
    11401 tgctgttcca ccgccgcagg accggcattt gaaggagcca atatccgcca tggtatgggc
    11461 ggaatcaggg gggctataaa cacaattagg cttaacgatg gccggctaaa gtacaccacc
    11521 attggcgatg aggcccctgt aggtatctgc ggttccggga tagttgatgc tctggcattt
    11581 ttgttggata caggtcttgt ggatgaaacc ggcaggttgc tggatgagga tgagatagaa
    11641 tctgaaaccg gaaaaagtct tgcccataga tttacagaga tagacgggca gggggccttt
    11701 gtattagtag acgctaaatc cagctctacg ggagagccca tagtcataac ccaaaaggat
    11761 ataagggaga tccagctggc taaggccgct atttcagcgg ggataaaggt gcttatacat
    11821 aggatgggca aagggatggg ggatgtaaca aggctctatc tggcgggggg atttggaagc
    11881 tatatagata agggcaatgc ggctaaaata ggccttattc caagggagtt acaaaacaga
    11941 attatcgcca taggcaatgg ggccggaacc ggggctatgc tgtcgctgtt atccgccgaa
    12001 caatttaagc gtgccgatct tatcgggaat atggcggagt atgtggaact gtcttccaca
    12061 ccggaatttc aggatgaata tgtaaacggc atgtacttca ccacctaagg tttatttcta
    12121 tggaaaaagt gatatagtac aaaaaatatt gaaattttcc aatacccggt gaagataagc
    12181 gtggtatcat ggaaccaata ctgcccgcat cgcctattta ctaacatcga tgaatccaat
    12241 ataatagggt tgtctgataa ttaaaatacg cagagagtgt gataccatgg ctttttggag
    12301 gagcaggggg gaaatagggg atgatgtggg gctcgatatg gatcttgcca tagaggccat
    12361 agggcactat aaccgagttg caggggtagg aataagtatt atagacacca ggggaaaacc
    12421 cctctacaat atcggctata ccgggctatg ctgcaaattt tgtagtggtg ttcgggatat
    12481 tttagatacg gaggacgatt actgcgatag ggttcacctg tatggcgggt atcaatccga
    12541 gagatttggg ggccgatata tatttttctg tcccttggga ttggtgcatt gggcttcgcc
    12601 cgtctcgtcc gaggggatgg ttaggggata cttattgtgc ggacctgctc ttatggtggc
    12661 accggaagaa tttttaatag aggatctcct gctcaaaaac cgtattgata aggagcactt
    12721 gggaaggcta aaagagaatt tagagcatat cccgttgata cccccggata gggttaccag
    12781 cctgtcggaa ctattattta tggtggcctc ccatttatcc gatgcagaat actttcagta
    12841 ccttagtcag gcagaatttc ataaccacca atccaatatc tcagaataca tacattccat
    12901 aaaggctgtg gccggtagtc agggagatgg agcagattac cccttcgaaa aggagaagga
    12961 acttttatcc tgcatagtcg ccggagataa aaaaggttcc cagactattc taaacaggat
    13021 tttagggcat atattctttt ccagcggcgg tgattttgaa accataaagg ctaggatatt
    13081 ggaacttata gtccttctgt ccagggcggc ggtagaaggc ggcgccgacg tagaggagat
    13141 atttggactc aattttaaat acctgagtac catccatagc tttaaaaccg tagaagagct
    13201 gacctattgg ctgtctaaaa taatgataag atttaccgat tgcgtattca atttgaaaaa
    13261 tattaagcat gtagacgtaa tatataaggc cattaactat ataaagcaga actatatgaa
    13321 aaagattacc ctggaggagg tggcctcaca cgtatacttg agcccatctt acttcagtaa
    13381 gatattcaaa gacgatatga aatgcaattt taatacctac gtaaaccggg ttagaatcga
    13441 acgaagcaaa caactattat tacaggatac aataaacctg gcggatattt cgaatatggt
    13501 cggttatgag gaccagagct atttttcaaa ggtttttaaa agggttgtcg gtattacccc
    13561 cggcagatat agacttggca ggggaaaatt aaaattttag gaggctgaat aatggctttg
    13621 ttagaagaga tctctaagta tttgcagcag ggaagggcga aaaacgtgaa ggaactggtt
    13681 caaaaggccg ttgatgagga cttggaaccg agggaaattc taaatgaagg gctattggat
    13741 ggcatgggca ttatcggtga aaagttcaaa aacaacgaag tctatgtacc ggaagttctc
    13801 atcgccgcca gagcgatgta tgccggaatg gaaatcttaa agcctatcct tacggagacc
    13861 ggcgtagaat ccataggtaa ggtggtaatc ggtacggtaa agggcgacct ccacgatata
    13921 ggcaaaaacc ttgtcaagat gatgatggag gggaggggcc tggaggttat agatctgggc
    13981 attgatgtgt cccctgaaag gtttgttgag gcggccaagg aaaatgatgc tcaaatcatt
    14041 gcatgctcgg cacttttaac cacaaccatg actgagatga aaaatgtggt agaggcggcc
    14101 aaggaatccg gtattcgaga ccaggtcacc atcatggtag gcggggctcc cgttacggat
    14161 aatttctgca agagcatagg tgcagatatc tataccgctg acgctgcatc agcggcggaa
    14221 gaggcggcaa gggtatgtaa aagcgcataa aaagaggata atcagccttt gatgctgatt
    14281 taatccgtat tggtaatggc tgccgaacgc taattttgtc actggccatc aacagcccat
    14341 tacctttgta aggcagtaaa actataaacc ggtataatgc gataaaacat ctacattgga
    14401 ttacggattc aaaagattca attatgagcg attcaatcat ataggaaaga ttcaattata
    14461 caggggtgga cgattatgtc cgcccctaag tttatcccgg gaaagctatt tgttatcccc
    14521 catccggctg gggaaggcta tcaccaatcc ccaagttgtg gaagatgtgg tacaataatt
    14581 gtaggagata attttcaata tcatctactg gggaggaaac catggagtat ctaaacggtg
    14641 ttgtagagag gattacctac tataacgaag attccggata cacggtaata aggcttagga
    14701 gcagagggta ttatgacctt gtgactgtcg ttgggaatat ggcgtctgtc catgtggggg
    14761 ccatcgtatc cctcaggggt agctggaagg ttgacagtaa atatgggaaa cagtttatag
    14821 cggaattcta cgaagaaaaa cttcccgcca gcgtggcagg catcgaaaaa tacttaggca
    14881 gcggccttat taagggcata gggcccatca acgccaaaag aatcgttaag gcttttggca
    14941 aggataccat agacgtcata gaagaagagc ccgacaggct tatggaggtg ccgggcatag
    15001 ggcctaagag gatagacatg ataaagaagg catggcagga gcaaagggag gtcaaaaata
    15061 tcatgttgtt tctccaatcc catggggtat ccacctccca cggtgcaaag atctataaaa
    15121 cctatggaaa tgaaagcctg aacgttgtca aggaaaaccc cttcagattg gccgatgata
    15181 tatggggtat cggttttaga actgcggata ccattgcgca aaaactgggc tttgacccac
    15241 aatccatgca gagatgccgc tcgggaatac tctatatcct aaaccagctt tccaacgaag
    15301 gccattgctt tgccacccgg gatcagctga taatggagac caaggagact ctggatctcg
    15361 atgatgccaa gatacaagaa tccatagaca taatggtggg cgacggatcc ctaatctatg
    15421 accaggaagc cctgtatctt ccccccttct tccacagcga ggtaggggta gcaaggcgga
    15481 ttagagagat tgcctccacc ccggcatccc tcgatatagt gggaatagac gaaattattc
    15541 ggggcataga ggacaggggc aggatcaaat acgaccaaat tcagcaagag gcaataaaaa
    15601 gatcagcatc ttctaaatta atggttctca ccggcggacc gggaaccggg aagaccttca
    15661 ccattttagg cataataagg gtttttgaaa ggataggggc caagatatat ttggcggcgc
    15721 ccaccggcag ggctgccaag aggatggaag aggccacggg aagggaagca aagaccatcc
    15781 ataggatgct ggaatataag ccgccccaag gctatatgcg aaacgtggaa aacaacccct
    15841 tagaatgtga tgtccttatc attgatgaga cctctatggt agacataatc cttatgtaca
    15901 acctccttaa ggccgtaagg gacgaaacca tcgtgattct ggtaggggac gtagatcagc
    15961 tgccctcggt aggagccgga aatgtgctgt cggatataat agcctccggc gaggtggatg
    16021 tagtgaagct gacaaggata ttcagacaag cccaaaatag tgccataatc accaatgccc
    16081 ataggataaa ccagggaatc ttccccatgt taaagggggg taagaatcgg gattttttct
    16141 ttatcagaag agaagatccg gaagagattg caggcgaaat ccgcagattg tgccgtgtga
    16201 gactgccaaa atattataag gtagacccca tagatgatat ccaggtccta tgccctatgc
    16261 agagggggga gacaggggcc gttaacatga accggattct tcaggaggcc ttaaataata
    16321 atagtcactt tattaggtac gggggcgcta cctataaggt gggggacaag ataatgcaga
    16381 ttagaaacaa ttatgataag aatgttttta acggggatat tggtaggatc tttagcatcg
    16441 acctagaaga caaaatcgtt aatatagact ttgacggaag tatagtcgaa tacgatagta
    16501 gcgaattgga cgagatcatg ctggcttatg ccgttacggt ccataaaagc cagggaagtg
    16561 aatacaaaat agttgttgcc ccattcacca cccaacattt tatgatgcta cagcgaaacc
    16621 tgttatatac ctgcataacc cgggcacgaa atattttcat aatggtgggc accgaaaaag
    16681 ccgtgggcat tgcagtgggc aataacagga ttgtaaagag aaacaccatg ctggacaaaa
    16741 gattaaaaga cccccggctt gatagaagat aagggggtct cttatgattt gagtttaaac
    16801 ttccattacc ggttctctcg catccttttc aaggtaaaca ccctgaacat agatatttac
    16861 ttcgtcgacg gtcagccccg tcattccctg aacagcttcg attacggcgg agcgtatatt
    16921 ctctgccacc tctgggatcc tgacaccata gttaacaaca atgctaatac cgatggtggc
    16981 tatctcgtct attaactcga cctttacgcc ccttttggag cttttcatcc ctaaagttga
    17041 cgtaatatca tctaccaaac cgctggacat agaagccacc ccaggaattt cagcagttgc
    17101 caatcctgcg atcacagcca ccacttcatt ggctatgacg gtctttccat aatcaccgct
    17161 actcatttcc ttgtcaattg ccatttctaa cacctctcct ctttcaatgg tgaaaaactt
    17221 tcggaacagt ggacggattt tatatgtaca tgggtatctg tacactactt atattataca
    17281 ccattggggg cattggttaa tcgtattttt ttaaaaaacc agtataatcc atgataatat
    17341 atcccttggt cccgccgccc cacaacactt cccgcttggt atttaaagcg tcgcccttat
    17401 aatagataaa caactccagc tataaaattt atcatcatga aattgcacaa gaccattgca
    17461 gttatcatgt cgataaattt agtttccgta ttgtttatct gtaattgcca ggagtgccat
    17521 aaaaaaacac ttgaaaataa cccagagaca tagtaaaata gtacatgctg atttccattt
    17581 ttattctgtt agattgcaaa tcatcaacaa caacagagat attagttttt tatgaataat
    17641 attattggga gttggcataa taattatagt taaatcaaat aaaattagtt aaatagtttg
    17701 ccgtgctaga tggggaggta gcggtgccct ataacctgca aaccgctata gca
//
LOCUS       DASRNX010000056         9426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_276800,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000056 DASRNX010000000
VERSION     DASRNX010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276800"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..2438)
                     /locus_tag="VFD89_02275"
     CDS             complement(66..2438)
                     /locus_tag="VFD89_02275"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZJ57048.1"
                     /translation="MLKHLKERNIQFGIICIICLSVLAGQLAYLTVEKGDELYAKSLV
                     KKRVELKLKGTRGNIVDRYGIPVAVNRQIYTVQLDRQLLPSNHGDLNNIILQMLEIIY
                     KNGDKDTLLNLMPIRLDPDTGKLRYTWDDEEKDIRDKRYDSWIRDIGIQDRLSPEEML
                     EYLRKERYEIDDEIADETAIHIISIRLNIHMRRFTQYEPIPVAEGIGPETVVQLETFA
                     PEMPGVQTLIGSGRYYPMDESFSQIVGYLGSISKDQIEDYKEQGYDISSDKIGQVGVE
                     SYGEEWLTGNKGDRLGKLTAEKDSFGKVIRVLDETSPQNGHDVVLTIDSQLQNSVGNI
                     LKEEIEKMREGLPPYEERNIAPLAEEGAAVVLDVNTGEVLSLVSYPSFDPNSSGKDGK
                     QFSLAYQGTFMPGSVFKMLIGIAGLMEGAITLNERIYDKVYYTKYDTKRPPSCNRKSG
                     HGWESYTDALKHSCNYFFYEVADRVGVEAITKWAELFGLDGMTGIEILSPDRDKNAIP
                     SEETKIRAHRPDMRRDITRIMKRYGYFDNELSEEQRDQVEQLIDFPLSKDRSREAGLE
                     EVRKLSEMIEAMGYTEKVNQAADEIRRDVLSYYKRWTPSDTIRAGIGQGYVDISPLAM
                     ARYIATIANGGKVLETHIVKKVVDSEGKTVKETQPTLIRELEVNQEYLEATLKGMWMV
                     ANDPNPTRGENDGGGTAAEYFKDLAPEITVAGKTGTAQTVSNDPNKNNAWFVSVTPYE
                     NPEIAVVVAIPNGRTAGNAAPVARRIIEEYYRLNGQRQENTLPTMNQVQP"
     gene            complement(2644..3159)
                     /gene="mreD"
                     /locus_tag="VFD89_02280"
     CDS             complement(2644..3159)
                     /gene="mreD"
                     /locus_tag="VFD89_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZJ57049.1"
                     /translation="MREMVILAILLLNLTVQSSIFPFVEILHVKPDSLLILTVSFALL
                     AGNPTGALVGLFGGLIQDILFGNNMGLHALQYMTVGYLVGILYGKLYVDKLFMPLLAV
                     MTSNMIKGIIMLGYNFFNQSGIPPGKAFIQIIVPETVYTLLLTPLIFYGIAGLYRNKF
                     MRKKWRFRKNI"
     gene            complement(3209..4042)
                     /gene="mreC"
                     /locus_tag="VFD89_02285"
     CDS             complement(3209..4042)
                     /gene="mreC"
                     /locus_tag="VFD89_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZJ57050.1"
                     /translation="MPRLFKNAPLIIVILAVIGLIVLMVYTEVGKERATMVENALGQA
                     LVPLQKGMYSASQFVYNLFSDIGERRNMKKDYEDLQARVADMEKELHMLEELERQNER
                     LKKLTLFLDENREMAVTGATVIAKNPGNWFNNITIDKGSKHGVELNMAVVTDQGLVGR
                     IIEVAEGWSKVRTIVDGKSAVSGIVQRNRDNGLLKGNNSLGSEDGLCRMIYLPEDSNV
                     TPGDKILTSGLGEIFPKGIYIGEVEKVIKDKRELYKTAIVRPGVDFQRLEEVIVVAND
                     S"
     gene            complement(4055..5086)
                     /locus_tag="VFD89_02290"
     CDS             complement(4055..5086)
                     /locus_tag="VFD89_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZJ57051.1"
                     /translation="MGFLNIFTKDMGIDLGTANTLVHVRGKGVIIREPSVVAIRSDTK
                     RVLAIGEEAKKMIGRTPGNIVAIRPMKEGVIADFDVTQEMLKYFIRKAIKRVFPLHPR
                     VVVCVPSGVTEVEKRAVEEAAKQAGAREAYLVEEPMAAAIGAGLPVQEPTGSMVVDIG
                     GGTSEVAIISLGGIVTSKSLRMGGNRMDDAIISYIKKEYNLMIGERTAEDIKISIGSA
                     APMTKEETIEVRGRDLITGLPKTLKITSTEIMDALREPVNSIVEAIKLTLEKTPPELA
                     ADIMDKGIMLTGGGALLNGLDRLVRQETGMPVQIAEDAIDCVALGAGRVVEEIETLKK
                     VALSSKSVG"
     gene            complement(5230..5811)
                     /locus_tag="VFD89_02295"
     CDS             complement(5230..5811)
                     /locus_tag="VFD89_02295"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017814075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZJ57052.1"
                     /translation="MKIILASASPRRKQLLEQMGLIFDIKTSDKKEQMPYGLAPEEIP
                     MELACNKAMDVASCVYQPAIIIGADTVVVKDKILGKPENEEEARQMLLKLQGKTHEVI
                     TGLAIIDTRTGKVNKDYEKTSVEMAPLTLSEIEYYVQTGEPMDKAGGYGIQGLAGIFI
                     SRIMGCYFNVMGLPIHRLWNMLKEYGINPLGNI"
     gene            complement(5816..6079)
                     /locus_tag="VFD89_02300"
     CDS             complement(5816..6079)
                     /locus_tag="VFD89_02300"
                     /inference="COORDINATES: protein motif:HMM:NF025572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HZJ57053.1"
                     /translation="MRRGRTKGPLMLFLFLLAGVIVGSIAGNIIANYSDAKIFNESFT
                     IGTQGMPAILDFNVIKLAFGLSLTVNFGTMLGVLLGIFLYYRY"
     gene            complement(6187..7821)
                     /locus_tag="VFD89_02305"
     CDS             complement(6187..7821)
                     /locus_tag="VFD89_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HZJ57054.1"
                     /translation="MNSSMIIMLAGGLGLFIYGMKMMGDGLEKAAGSRLKRLLEILTE
                     NRLMGLLVGAIVTAVIQSSSATTVMVVGFVNAGIMTLGQAVGVIMGANIGTTMTAFLI
                     SLNLTDIAPIIVFVGVGLVFFSKRKFVKSIGEILVGFGILFIGMGFMSDSMVPLRTNA
                     GFQRLLVSMSHPILGIIAGTIMTVVLQSSSAAVGILQALATQGLIGLNSAIFILFGQN
                     IGTCITAILASIGTNTNAKRAAFIHLSIKVIGTSIFMVAMALGVPFVDFILKVSPANS
                     PMSQVANAHIGFNVITAAILFPFGNILVNLSKNLVHGKKDEPDEKGLIYLDKRILETP
                     PIAIAQILKEVNRMADMAVGNVRLAMDAFFRREQAIIDGVYKKEEIINFLNQEITHYL
                     VMCNGLGLPESDLHLISGLYHVVNDIERIGDHAENLTEYAEYSIENKLSYSEIAEEEL
                     GEMYEKVMEMLNNTVHALRERDHELAELVMKEEDVIDTLKDRLRKSHIGRLNAQKCSA
                     AAGVIFLDIVTNFERIADHATNVAQSVLDEGRSRNY"
     gene            7996..9201
                     /locus_tag="VFD89_02310"
     CDS             7996..9201
                     /locus_tag="VFD89_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HZJ57055.1"
                     /translation="MAQFNITITEELLHGLFLSNGKDEAFSKLLEEIFNQVLLAQSSE
                     QIGAEPYERTDDRKAYRNGYRDRPMTTRVGTLTLRVPRHRNGLFSTELFARYQRSEQA
                     LVLAMMEMVVNGVSTRKVENITEELCGKKFSKSTVSELCKGLDPIVEAFKTRPLKAHY
                     PFVQVDAIYLKVRENGRVASKGLLVAVAINENGYREIIGFNVADSETEDSWGTFFHSL
                     KERGLKDVRLVTSDNHKGLVNAVKKHFQGASWQRCQTHFSKNVIDSTPKSLQPEIKED
                     LRKLYDAVDIENARKYRDKIYTKYEAKAPKAVAVLDEGFDDVTAVLSLPLYYRKRLRT
                     TNGVERLNQEIRRRERVIRIFPNEASVIRLMGALLMEQDEKWQTGRKYLDMALYYREQ
                     DETANQEVA"
BASE COUNT         2470 a         2379 c         1833 g         2744 t
ORIGIN      
        1 attatattcg tcaaaattct ttaaaaccct ctatgtttcc ccaatattat ttgtttatgg
       61 aaaagtcaag gctgcacctg gttcatcgtc ggcaaggtat tttcttgtct ttggccgttt
      121 aatcgataat attcctcgat aatcctacgg gctaccggtg cggcattccc cgcagttctg
      181 ccgttgggta tcgccactac caccgcaatc tcgggattct cgtagggtgt cacagataca
      241 aaccaggcat tgtttttgtt gggatcattg ctcacagtct gggcagtacc cgtctttcct
      301 gctactgtaa tctccggggc taaatccttg aagtactcgg ctgccgttcc tccgccatca
      361 ttctcgcccc tggtgggatt aggatcattg gctaccatcc acataccctt tagggtggcc
      421 tctaaatatt cctggtttac ctccaattcc cttattaggg tcggctgggt ttccttaaca
      481 gtcttacctt ccgaatccac taccttcttc actatatggg tttctaagac cttcccaccg
      541 ttggcgattg tcgctatata ccttgccatg gccaatgggc ttatatccac atagccctgt
      601 cctatcccgg cccttatggt gtcagaaggt gtccatctct tgtaatagga tagcacgtcc
      661 cttctgatct cgtccgccgc ttgattgacc ttttctgtgt atcccatggc ctctatcatc
      721 tctgacagct tcctaacttc ctccaaaccc gcctctctgg aacggtcctt ggacagggga
      781 aaatctatca gctgttccac ctgatccctt tgctcctcgg aaagctcgtt atcaaagtac
      841 ccataccttt tcatgattct ggtaatatcc cttctcatgt ccggcctatg ggccctaatc
      901 ttggtttcct cgctgggaat ggcgttttta tccctgtccg ggcttaaaat ctctatccct
      961 gtcatgccat caagcccaaa cagctctgcc catttggtta tagcctcgac gcctacacga
     1021 tcggccacct catagaagaa atagttgcat gaatgcttta gggcatctgt atacgattcc
     1081 caaccatgac cggatttgcg attacagctg ggcggccttt ttgtgtcgta tttggtatag
     1141 taaaccttat catagatcct ctcgtttaag gtgatagcgc cctccatcaa ccccgctatg
     1201 ccgattagca ttttaaatac ggatccgggc ataaatgtgc cttgataagc cagggaaaat
     1261 tgctttccgt ccttaccgga gctgttgggg tcaaaggatg ggtagcttac cagggataat
     1321 acctcccccg tatttacatc caatacgacg gcggcgcctt cttccgccag tggggcaatg
     1381 tttctctctt cgtacggggg caacccttcc ctcatcttct ctatttcctc ttttagaata
     1441 ttgcccacgg aattttgaag ctggctatct atggtcagta ccacatcatg gccattttgc
     1501 ggtgaggttt cgtcgaggac ccgaataacc tttccgaatg aatccttctc cgccgtcaat
     1561 ttccccagcc tatcgccttt atttcctgtg agccattcct ccccgtagga ttccacacca
     1621 acttggccta tcttatcgct ggagatatca tatccttgct ccttgtagtc ctcaatttga
     1681 tccttgctga tgctgccgag gtaacccact atttgggaaa aggattcgtc catgggataa
     1741 tagcgcccgc tgcctatgag ggtttgaacc ccgggcattt cgggtgcaaa ggtttcaagc
     1801 tgtacaacgg tttccggacc gataccctca gctaccggta tcggctcata ttgggtaaac
     1861 cgtctcatat gaatattgag cctgatggat ataatatgga tggctgtttc atcggcaatt
     1921 tcatcgtcta tttcatagcg ctcttttctg aggtattcta acatttcctc cggcgacaac
     1981 cgatcctgga tccctatatc cctaatccaa ctgtcgtatc gcttatcccg tatatccttt
     2041 tcttcatcgt cccatgtata tcgaagctta ccggtatcag gatctaacct tatcggcatt
     2101 agattcaaca atgtgtcctt atccccattt ttatagatga tctctaacat ctgtaatatt
     2161 atattattaa gatccccatg gttcgacggc agcaattgcc tgtccaattg taccgtataa
     2221 atctggcgat tcacagccac gggaatgccg tacctgtcta caatattgcc ccttgtgccc
     2281 ttaagcttca gttccactct cttcttaacg agggactttg catacagctc gtcccctttt
     2341 tctacagtaa gatatgccag ttgccctgct aatacggaca ggcatataat acagattata
     2401 ccgaattgta tatttcgttc ctttaggtgt tttagcatga tttctccctt actccgtcaa
     2461 gataagtaac ccgatttgcc tgccaatgca ggcttactga tgatattgta ccatgtttta
     2521 ataccataaa gaagtggatt aaaatactaa ataaaaaaga atagatgtcc tctattctta
     2581 agcaatctgt tacactggtt aaatatatcg gaaatggcta ttgaactaat acaatacgtt
     2641 aaattatata ttcttacgaa aacgccattt ctttctcata aacttatttc gataaagccc
     2701 ggcaatccca taaaaaatca acggtgttaa aagtaaagtg tacacggttt ccgggacaat
     2761 gatctgtata aatgccttgc ccggtggtat accggactga ttaaagaagt tatagccaag
     2821 catgattatc ccttttatca tattggatgt cattaccgcc agtaggggca taaacaattt
     2881 gtccacatac agttttccgt ataatatacc caccaaatac cccactgtca tatactggag
     2941 ggcatgaagc cccatattat tgccgaaaag aatatcctgt atcagccccc caaacaatcc
     3001 tacaagggca cctgtggggt tgccggccaa cagagcaaag gaaaccgtta ggatcagaag
     3061 ggaatcgggc tttacatgga ggatttcaac gaagggaaag atggaagatt gaaccgtcaa
     3121 gttcagcagc aatatagcca atattaccat ttccctcatg caaaaccacc ccacaacaat
     3181 ttaaaatgaa tgcttcccca aagccgatct atgaatcatt ggctacaact attacttcct
     3241 ctaatctctg aaaatctacc ccaggcctga cgatggctgt cttgtagagt tcccttttat
     3301 cctttatgac cttttctact tcgcctatat atatgccctt gggaaatatc tcccccaatc
     3361 cggaggtaag aatcttatct ccgggcgtaa cgttagaatc ctccggaagg tatatcatcc
     3421 tgcacaggcc atcctctgac cccaagctat tgttgccctt tagcaaaccg ttatccctat
     3481 ttctttggac aataccgctc acggcgctct ttccgtctac gatagtacgc acttttgacc
     3541 agccttctgc tacttcaatt atcctgccta ccagcccctg gtcggtcacc acagccatat
     3601 taagctctac cccatgcttt gatcccttat cgatggttat attgttaaac caatttccgg
     3661 ggtttttagc gatgacggtg gcgcccgtaa ctgccatttc tctgttctca tctaaaaaca
     3721 atgtcagttt ttttagccgt tcattttgcc tttccaattc ttccagcata tggagttcct
     3781 tctccatatc cgctaccctg gcttgcagat cttcataatc ttttttcatg tttcgtctct
     3841 ccccaatatc ggagaagaga ttatatacaa attgggaagc tgaatacatc cctttttgca
     3901 gggggaccag cgcctgtccc aaagcatttt caaccattgt ggccctttcc ttaccgactt
     3961 ctgtatagac catcaacacg attagcccga tcacggcaag gattactatt atcaaaggag
     4021 cattcttaaa caatcgaggc actcgttata ccccctatcc gacactcttt gaggataggg
     4081 ctaccttctt caatgtttct atctcttcaa cgacacggcc ggcacctaag gctacacaat
     4141 ctatggcatc ttcggcgatt tggacgggca tacccgtttc ctgtctaacc aacctgtcca
     4201 gcccgtttaa cagagcccct ccgcctgtta acattatccc cttatccatt atatcggctg
     4261 ccaattccgg cggcgtcttt tctaaagtga gtttgatcgc ctcaacgata ctatttacag
     4321 gctccctcag agcgtccata atctccgtgg acgtgatctt taaagtttta ggcagaccgg
     4381 taatcaggtc acggccccga acctctatgg tttcttcttt tgtcattggc gccgccgatc
     4441 ctatggatat tttaatatcc tcagcggttc gctcccctat catcaaatta tattcctttt
     4501 tgatatagga tataatggca tcatccatcc tatttccgcc cattcttagg gatttgctgg
     4561 tgacaattcc acccagggaa ataatcgcca cttcactagt tcctccgcct atatcaacga
     4621 ccatgctgcc ggtgggctct tgtacgggca atcccgcccc aatggcggcg gccatgggtt
     4681 cttccaccag atatgcttca cgggcacctg cctgcttggc agcttcttcg actgcccgct
     4741 tttctacctc ggttacccca gagggaacgc agacgactac ccttgggtgt aacggaaaaa
     4801 ccctttttat cgccttgcgg ataaaatact tgagcatctc ctgagttaca tcaaaatcgg
     4861 cgatgacacc ttccttcatg ggtcgaatcg ctacgatatt tcccggagtt cttccgatca
     4921 tcttcttggc ttcctcgcct atggccaaaa cccttttagt atcgctgcga atggccacta
     4981 ccgacggctc ccttattatt acgccttttc cccgaacgtg caccaatgta ttggctgtac
     5041 ctaaatctat ccccatatcc ttagtaaata tatttaaaaa tcccatagtc aaattatgct
     5101 ccttcctatt tatcccttta gctgtcagag gcttaggatc ggtatcctaa aaggtgtacc
     5161 caagaaataa ttatagcctt cctgtcttaa ttatggccaa tattcggcta tttgataacc
     5221 atactgaaat tatatgttac caagcgggtt tatcccatat tccttgagca tattccatag
     5281 acggtgtatg ggcagcccca taacattgaa ataacaaccc attatcctcg aaataaaaat
     5341 accggccaac ccttggatgc cgtaacctcc cgccttatcc attggttccc cggtttgaac
     5401 gtaatattcg atctcagaaa gtgttaacgg agccatctct actgatgttt tttcgtagtc
     5461 tttattaact ttccccgtcc gtgtgtcaat tattgcaagc ccggtaatca cctcatgggt
     5521 tttaccctgt aatttgagga gcatttggcg ggcttcctct tcgttctcgg gctttcccag
     5581 gattttatcc ttcactacaa cggtatcggc acctattatt atagccggtt gatatacgca
     5641 tgatgcaaca tccatagcct tattacaggc taattccatg ggtatctctt ccggagccaa
     5701 accatagggc atttgttctt ttttatcgct ggtctttata tcaaatatta agcccatctg
     5761 ctctagtaat tgctttcttc tgggagaagc cgaagccaaa ataatcttca tgatattaat
     5821 atctataata cagaaagatg ccaagcaaaa caccaagcat ggttccgaag tttacggtca
     5881 aggatagccc aaaagctaat ttaattacat taaaatccaa tatagccggc ataccctggg
     5941 tccctatggt aaagctttca ttaaaaatct tggcgtctga ataatttgca ataatattac
     6001 ccgctatgct gcctacaata acccctgcaa gtaaaaatag gaataacatt aatggtccct
     6061 tggttctccc ccgtcgcaag aagagtcccc ctttcaatgt caagaaaaat aaaactatta
     6121 taagcataat agttatcatt aaaagtataa catttttatg gggaaataca agttatttta
     6181 cgaaaattaa taatttctcg acctgccctc gtccaatacc gattgggcga cattggtcgc
     6241 atggtctgct attctctcga agttagtaac tatatcaaga aatatgacgc cggcggccgc
     6301 ggaacatttt tgggcgttta atctgcctat atgacttttc ctgagcctgt cctttaaggt
     6361 atcaattaca tcctcttcct tcatcaccaa ctctgccaat tcgtggtcgc gctctctcag
     6421 tgcatgaacg gtgttattta gcatctccat aaccttttca tacatctcac ccaattcttc
     6481 ctcggctatc tcagaatagc tgagcttgtt ttctatactg tattctgcat actcggtcag
     6541 gttttcggcg tgatcaccga tcctctcgat atcatttacc acgtgatata agccactgat
     6601 gaggtgtagg tcgctttcag gtaatcccag accattacac ataacaaggt agtgggttat
     6661 ttcctggttc aaaaagttta taatctcttc tttcttatat accccatcaa ttatagcctg
     6721 ttcccttcgg aagaaagcat ccatggcaag acgtacattt cccacggcca tatcagccat
     6781 cctgttgacc tcttttagga tttgcgcgat tgcaatagga ggtgtttcca gtatacgctt
     6841 gtccaaatag atgagtccct tttcatccgg ctcatctttt tttccatgta ccagattctt
     6901 cgacaaatta accaatatat taccaaatgg gaacaaaata gcagccgtta tcacgttaaa
     6961 gcctatatgg gcgttagcaa cctggctcat gggactgttt gcaggagaaa ccttcaatat
     7021 gaaatccacg aagggtaccc ccagcgccat agccaccatg aagatggatg tcccaataac
     7081 tttaatggaa agatggataa acgctgctcg tttcgcgttg gtgtttgtac caatactggc
     7141 cagtatagca gttatacacg tgccaatatt ctggccaaaa aggatgaata tcgcgctgtt
     7201 tagcccaatt aaaccctggg tagccaatgc ctggagtatt ccaacagcag cacttgaact
     7261 ttgcaaaact acggtcatga ttgtccccgc tataatgcct aagataggat gggacatgct
     7321 aaccaataaa cgctggaagc ccgcattggt tctgaggggt accatggaat cggacataaa
     7381 ccccattcca ataaataaga tgccgaaccc gactaggatc tcaccgatac tctttacgaa
     7441 ctttcgtttt gagaagaaca caagacctac accaacaaat acaattattg gcgctatatc
     7501 ggtcaggttt aaagaaatta ggaatgcggt catagtagtt ccgatgtttg cacccataat
     7561 aacccctacc gcttgaccca gggtcattat acccgcattg acaaagccta ctaccatcac
     7621 cgtcgttgcg ctggaacttt gaataactgc tgtaacaatt gccccaacca ataaccccat
     7681 caacctgttt tcggtgagga tttctaaaag tctttttagc ctgctacctg cggccttttc
     7741 caagccgtcg cccatcatct tcattccata aatgaatagg cccaagccgc ctgctaacat
     7801 tattatcatg ctagaattca taatacctcc ttattaagta aaactaatat ttgaacaaag
     7861 gcccaataat ggtttatctc accttgctaa agattaagtc cgtataatgg tgtaaataga
     7921 aaaatgagtt gagccaaggc tctacctcgg ttaaaatata agtactaccc aataccaaac
     7981 cgaggaggaa aaaccatggc tcagttcaat attacaataa ccgaagaact tttgcacgga
     8041 ctttttttat caaatggaaa agacgaggcc ttttccaaac tactggaaga aatatttaac
     8101 caagtgttgc tggcacagtc atcagaacaa ataggggcag aaccctatga aagaactgat
     8161 gatagaaaag cctaccgtaa tggctacagg gataggccta tgacaaccag ggtaggtacc
     8221 cttaccctaa gggttcccag gcatagaaac ggcctattca gcacagagtt atttgccagg
     8281 tatcaacgaa gcgaacaggc tctggtgctg gcaatgatgg agatggtggt aaacggcgta
     8341 tccactagaa aagtagaaaa tattacagaa gaactctgtg gcaagaaatt ctccaaatcc
     8401 accgtttcag agctttgcaa gggtctggat cccatagtgg aagcctttaa aaccaggccc
     8461 ctgaaggccc attatccctt tgtacaggta gatgccattt accttaaggt tcgagaaaat
     8521 ggccgtgtcg cgtccaaggg actccttgtg gcagtagcta tcaatgaaaa cggctatagg
     8581 gaaatcatag ggtttaatgt tgccgatagc gaaactgagg atagttgggg taccttcttt
     8641 cattccctca aagaacgggg gcttaaagat gtcaggctgg ttacctctga taaccacaaa
     8701 gggcttgtaa atgcagtcaa gaagcatttt cagggggcca gctggcaacg ctgtcagact
     8761 catttttcta agaatgtaat tgatagcacg ccaaaatcct tacagcctga gatcaaggag
     8821 gatttaagaa agctttatga tgccgtagat attgaaaatg cccgtaaata cagggataaa
     8881 atatatacca aatacgaggc aaaagcgcca aaagctgtag ctgttctgga tgagggtttc
     8941 gatgatgtca ccgcggtttt atccttgccg ctttactacc gtaaacgcct gcgtaccacc
     9001 aatggagtag aaaggctaaa ccaggagatc agaaggcgtg agcgtgtcat taggatattt
     9061 cccaatgaag cttctgtcat acgtttgatg ggggcgctac ttatggaaca agatgaaaag
     9121 tggcaaacag ggcgtaagta ccttgatatg gctctgtact accgggagca agatgaaact
     9181 gccaatcagg aggtagccta ataaagagcg catttattcc acggtcctat tgacacgggg
     9241 gcccctgtcc cctctggaca cccctttatg gcggaattag ccctaggggg cggttacttc
     9301 cgccccgaga ttataccaga gggggctccc ccgtatcaat aggctttcgc ggcataaaag
     9361 cttacgtttt tcaaggtgcg aaattccgtt tttttaaaaa agcagccgga atattttact
     9421 accgag
//
LOCUS       DASRNX010000057         4789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_383094,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000057 DASRNX010000000
VERSION     DASRNX010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383094"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1795
                     /locus_tag="VFD89_02315"
     CDS             164..1795
                     /locus_tag="VFD89_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015847052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HZJ57056.1"
                     /translation="MRNLKGIMALLLVMLLTLSILAGCGQKPTKETEKTPDKVVDDQD
                     KDKDKDDEAEEDGQDQDGDVAIDTSKFVEVVWYHLGDPPTNDQLDLAAAEWNKILEDK
                     VNAHLKINFIEWADYLTKYNLMLATKEVLDMINTASDWLDMWPNAAKGAFMDITDLLP
                     KYAPQTWSEVPEENWEQCKFDGKIITIPEDQYTQWVNHGFYYRGDWAEEFGITEPIRH
                     WDTLGEYLQKVKDVKGIIPYDVVGSKANEILDYWMGAYTDAICLEVPGNIFYSKTYDD
                     LYTVYSPIFDDTMVEMAKKMKEWGDAGYWRNDVLNYTGDTREALKAGQTGLDGHHTQT
                     YAGLRVDMDEKQPGSDLQMFPMHLQGGKNLVEMSITHGAQSIAASSENPERALMVYDL
                     MRNDETLYRLLNFGREGVNYEIVDGKRTRPADFDETEHGFYSNYWGGRMDIFEMALPS
                     DRVYDKIGDLYAIYDQYKKPYPYGGFVFDKTSIETELSALNDVKTRHQGPIAFGKAGD
                     PEAAVEKFREEMKSAGYDTVLEEVQKQIDEWKASR"
     gene            1913..4312
                     /locus_tag="VFD89_02320"
     CDS             1913..4312
                     /locus_tag="VFD89_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018930621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HZJ57057.1"
                     /translation="MEMTDIRTILNINPGWKFHLGDEPGAWQKGFDDGGWRTVDLPHD
                     WAVEHPFDRGYSSGAGYLPGGVGLYRKDFHLGEEWEDKRIYVKFDGVYNNSMVWCNGY
                     YLGKRPSGYTEFIYDITDFVCFGDTKNVLTVRVDHKHVADSRWFTGSGIYRKVTLIIK
                     DKVCIDNYGVFVSTKSIDDSGAELQIQTTIINTADQDEDIVVNYSVKDRDGNQVASSK
                     AGYGVLAHNSIADKRVILVKDPVLWSTGSPYLYDFITDIIRDGEVIDRKVIKIGIREI
                     EFHPRRGFFLNGQNMKIKGVCIHHDAGCLGAAVREKVWERRLKILKETGCNAIRMSHN
                     PHMPELYGLCDSMGFLIIDEAFDEWEGVKNKWAKGHNVYPPAHYGYYEDFPEWHERDL
                     TTMVLRGRNHPSIILWSIGNEIDYPNDPYGHPLFQTVTGNNDANKPAIERIYDENKPN
                     AERLVPIAKRLVSIVKRYDLTRPVTAALALPELSNITGLADCLDVVGYNYKEHLYEED
                     HKKWPDRVLIGSENGKELSQWLAVRNNDFISGQFLWTGIDFLGETRGWPSHGSEAGLV
                     DVAGFKKPGYYFRKSLWSDMPMVKLFAGYAHELLEHRRYMLYNKHLKSSWNFVEDSEV
                     EVICLTNCHKTELFLNGRSLGIKKVADHDGAYLTWKIPFEKGELKAVSTDARGRVYED
                     ILKTTGAAAGISLTPTDNSIAADGLDMAHVILGIIDSDGNPVDNAHDLIEVSLEGPGR
                     ILGLENGNLEDTMPYGLPYRRVHNGKLLIYIASTPEKGIIKVRAGARGLKSCQISIIS
                     Q"
     gene            4486..>4789
                     /locus_tag="VFD89_02325"
     CDS             4486..>4789
                     /locus_tag="VFD89_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZJ57058.1"
                     /translation="MKKNVTIYTIAGEAGVSVATVSRFLTGKANVKESNREKIERIIK
                     KYNFRPNAIARNLSNQETKTLGFIMPDVTAPFYGTVFLEAERVALEMGYSILLCNTM"
BASE COUNT         1435 a          914 c         1269 g         1171 t
ORIGIN      
        1 aacagggcta tacagtagag gcgaactgga cacgcctgta cgcatcaaaa tagcttaggc
       61 tacagaggcg taaatgagtt catttgaact ctagaacccc gtggctttag tcatgggagg
      121 ttcagaaatg gttgccaaaa acaatattgg ggaggtatgt gtgttgagaa atctaaaagg
      181 tattatggca ttgctgctcg ttatgctact gacattgtca atactcgcag gatgtggtca
      241 aaagccaacc aaggaaacgg aaaaaacgcc ggacaaggta gtagatgatc aggataagga
      301 taaggacaaa gatgatgagg ccgaggagga tggccaggat caagatgggg atgtagctat
      361 tgatacatcc aagttcgtcg aagtggtttg gtatcatctg ggggatccgc ccacaaacga
      421 tcagttggat ttggcggcgg cggaatggaa caagatctta gaggacaagg taaacgcgca
      481 ccttaagata aacttcatcg agtgggcgga ttacctgacg aagtataatt tgatgctagc
      541 gacaaaggaa gtactggaca tgatcaatac cgcatcggat tggttggaca tgtggcctaa
      601 cgccgcaaag ggcgcattta tggatattac cgatctgttg cccaaatatg ccccacagac
      661 gtggtcagaa gttccggaag agaattggga acaatgcaaa tttgatggca agattattac
      721 gatccccgag gaccaatata cccagtgggt aaaccatgga ttctattatc gcggtgattg
      781 ggctgaggaa tttggcatta ccgaacccat ccgccattgg gataccctgg gggaatacct
      841 gcagaaggta aaggatgtta aagggatcat cccctacgat gtagtgggaa gtaaggccaa
      901 tgaaatcctt gattactgga tgggcgcata tactgacgcg atttgtttgg aggttccggg
      961 aaatatattc tattccaaaa cctacgatga tctgtacacg gtatattccc ctatatttga
     1021 tgacactatg gtagaaatgg ctaaaaagat gaaggaatgg ggagatgccg gctattggag
     1081 aaatgatgta ttaaattaca ccggcgatac ccgcgaggcc ctcaaggcag gacaaaccgg
     1141 gttggacgga catcacactc aaacatatgc cggccttagg gtggatatgg atgaaaaaca
     1201 gcccggctct gacctgcaga tgttcccgat gcacctacag ggtgggaaga atctggtgga
     1261 aatgtctata acccatggtg ctcaatccat tgctgcatct tcagaaaatc cggagagggc
     1321 attgatggta tatgacctta tgagaaatga cgagaccctc tataggttgc ttaactttgg
     1381 gagagaggga gtaaactacg agatcgtaga cggcaaacgc accaggcctg cagattttga
     1441 tgaaactgaa cacggtttct attcaaacta ttggggcggg cgtatggata tatttgaaat
     1501 ggcactcccc tcagacaggg tatatgataa gataggtgat ttgtacgcta tatacgatca
     1561 gtacaagaaa ccatatcctt atggcgggtt cgtatttgac aagacctcca tagagaccga
     1621 gctttctgca ttaaacgatg ttaaaacaag gcatcagggt ccaatagcat ttggtaaggc
     1681 gggagatcct gaagctgccg ttgaaaagtt cagggaggaa atgaagagtg cgggctacga
     1741 tacagtcctg gaagaggttc aaaaacaaat tgatgaatgg aaggcatcac gataatcact
     1801 gtaaatagac tgaccacata ggcttttggg gctgccttcc cggcggccct tttttacgga
     1861 atacgattac tcggatatat taaagaaggc taaattgttt aggggggaaa ccatggaaat
     1921 gaccgatatt agaacaattt taaatataaa tccgggctgg aaatttcatt taggtgatga
     1981 gccgggggca tggcagaagg gtttcgatga cgggggatgg cgcaccgttg atctgcccca
     2041 tgattgggcc gtcgagcatc cctttgatag aggatattcc agtggagcag gttacctacc
     2101 cgggggggtt ggtctctata gaaaggattt tcatctaggc gaagaatggg aagacaagag
     2161 aatctatgtg aagtttgacg gtgtatacaa caacagtatg gtttggtgta atggctatta
     2221 tctgggaaaa aggccctccg gttatacgga gtttatatac gatataaccg acttcgtctg
     2281 ttttggggat acaaaaaatg tcctaaccgt cagggttgat cataaacatg ttgcagattc
     2341 ccggtggttt acaggatcgg ggatatacag gaaggtaact ctaataataa aggataaagt
     2401 atgtatcgat aactacggcg tctttgtctc cactaaaagt atcgatgata gcggggcaga
     2461 attgcagata caaactacca taataaatac tgcagaccaa gatgaagata ttgtagtaaa
     2521 ttattctgta aaggaccgcg atgggaatca agtcgcctca tcaaaggcgg ggtacggggt
     2581 cttggcacac aatagcattg cggataagcg ggtcatctta gttaaggatc ccgtgctttg
     2641 gtctacaggg tctccctatc tatacgattt tataacagat ataataaggg acggagaagt
     2701 tatagatcgg aaagtgataa agatagggat aagggagata gaattccatc caaggcgggg
     2761 cttcttttta aacggccaaa atatgaagat aaaaggcgtg tgtattcatc atgatgccgg
     2821 atgtctgggc gccgcggtta gggaaaaagt atgggaaagg cggcttaaga ttttaaaaga
     2881 gacgggctgt aacgccatac gtatgagcca taatccccac atgccggaac tatatggtct
     2941 gtgcgactct atgggcttct tgatcattga cgaggccttc gacgaatggg agggggtaaa
     3001 aaataagtgg gcgaagggac ataatgtata tccgcctgcc cattacggct attacgagga
     3061 ctttccggaa tggcatgaaa gggatctcac aacgatggtg ttaaggggga gaaatcatcc
     3121 ttccataatt ctctggagta taggaaacga gatagattat ccgaacgatc cgtatggtca
     3181 cccgctcttt caaaccgtaa ccgggaacaa cgatgccaac aaacccgcca tagaacgcat
     3241 atacgatgaa aataagccca atgccgaaag gctggtgccc attgccaaaa ggctggtatc
     3301 catagttaag agatatgatt taacaagacc ggttaccgca gccctggccc tcccggagct
     3361 ttccaatatc acgggtcttg cggattgtct ggatgttgtc ggatacaatt ataaggaaca
     3421 cctctacgag gaggatcata aaaaatggcc cgatagggtg ctcattggaa gcgagaatgg
     3481 caaggaactc agtcagtggc tcgctgttag gaataatgac tttatttcag gacagtttct
     3541 gtggaccggc atcgactttt tgggcgagac caggggatgg ccttcccatg gctccgaagc
     3601 ggggctcgta gatgttgccg gctttaagaa gcccggctat tattttcgaa aaagcctatg
     3661 gtcggatatg cccatggtta aactattcgc aggttatgcc catgagttac ttgaacacag
     3721 aagatatatg ctatataata agcacttgaa atcctcttgg aattttgtag aggacagcga
     3781 ggtagaggtc atttgtctaa ccaattgcca taaaactgaa ctattcctaa acggaagatc
     3841 cttagggata aagaaggtcg ccgatcatga tggggcttat ctgacttgga agataccctt
     3901 tgaaaagggc gaattaaagg ctgtctccac agatgcccgg ggcagggtat atgaggatat
     3961 cctaaaaacc actggggcag cggcaggaat aagcctgacc cctacggata atagtattgc
     4021 agccgacggc ttggatatgg cccatgtgat attggggatt atcgattccg atggaaaccc
     4081 tgttgataac gcccatgact taattgaggt atccctcgaa ggaccgggtc ggatcttggg
     4141 gctggaaaac ggtaatcttg aagatacgat gccctatggg ttgccatata ggagggtaca
     4201 taacggaaag ctgttgatct atatagcatc gacacctgag aagggcataa tcaaggtcag
     4261 ggctggggca aggggattaa aatcctgtca aataagtatc ataagccaat agtaaatggg
     4321 ttatggttgt tcaataaggc cgcttttgtg ggattattag cggcctttcc cttagggact
     4381 ttttgaaacc ccgggtcctt gatacgaagc ccttttgccc agataatttg gactaatgca
     4441 gtgaaaccaa aggttaaaac aagtttataa aacgggtggt catccatgaa aaagaacgta
     4501 actatttata ccatagccgg agaggccgga gtctctgtgg ctaccgtttc cagatttttg
     4561 accggcaaag ccaatgtcaa agagagcaac cgagagaaga tagaaagaat tattaaaaag
     4621 tataatttta gaccaaatgc cattgcgcgc aatctctcca atcaagagac caagaccttg
     4681 ggctttataa tgcccgatgt tactgcaccc ttttatggta ccgttttttt ggaggccgaa
     4741 agggttgcat tagagatggg ctatagtata ttgctatgca atactatga
//
LOCUS       DASRNX010000058         8210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_489827,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000058 DASRNX010000000
VERSION     DASRNX010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489827"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..704
                     /locus_tag="VFD89_02330"
     CDS             207..704
                     /locus_tag="VFD89_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57059.1"
                     /translation="MDTRIRPGLKIHIKSRPTHSHLKLIVLFVLILLMAVNVLGCKKS
                     APVDGENVTDSGGDVSNSPDVDESKGKDMLPQLERPLLESGDDETGQGPMLDPFSGSL
                     TLTGILTSKFTKDNIAIIEYGNTSYIVNQDDIIAEYWKVGSIKNTGVLLKHDDVELVL
                     EFSGK"
     gene            736..2238
                     /locus_tag="VFD89_02335"
     CDS             736..2238
                     /locus_tag="VFD89_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZJ57060.1"
                     /translation="MRFSKRLLYKTICLAILTSVLATTGIPPSHRQGISYGFDTDNRD
                     AYTEYIVKRGDNLSDIAKEFETDIPTLIRDNNIKNKNHIYVGQKLKIKDSVTDDKDMV
                     DTEADTDDEAASLEQDSHNSSGYESRDEDLISLDMRDADIRDVISSIAYYLDTYAILL
                     EEPIRVNFQVENTKPYKALELLLQTTGLSYIEDDSLLIIGKADKLQEEFYGQMALTRF
                     NLKHISSESLKPLIGELGIPLEIVTIDKNPKAIWVQGPPQSLSKVKELITMLDKAENA
                     TEFSRLTSVKLKNTTTDKLASVIDDLDMPVKVITLPNIMGTLWLQGTKEGVENAKSVI
                     SALDIVETSSDQYTLFLFKLRNIAAKDAAERLELFGYEGVKTIAFHYPEFGKDLLILC
                     PAGMKTRVTTALEGLDLRGLGDNPTHIMLPVYSASGPNAWDVLEARRSLLIMLMDELK
                     DDRDLIDVSDDILTKDGEEYRVLLVKSTVEVINKVREMVSLIDSPNTDAP"
     gene            2286..3392
                     /locus_tag="VFD89_02340"
     CDS             2286..3392
                     /locus_tag="VFD89_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HZJ57061.1"
                     /translation="METEKDRLFGGTGKYSLDLDALFKITVERHASDLHLLCGAPPHI
                     RVDGDLIRLDMPIITPDSLMNLLYPILNNRQIESLEKDLELDFAYSLPGISRFRGNVM
                     FQRGTLGAAFRVVPYNIPSIDSLGLPKGVRDLCSLSQGLVLVTGPTGSGKSTTLAAML
                     DYIGNNRRLNILTLEDPIEFLHKHGNSIISQREVGSDTHSFSTALRHALRHDPDVILI
                     GEMRDLDSISIALTAAETGHLVLSTLHTQNAPLTINRIVDVFNADRRSYIRQQLSNSL
                     QAIVSQQLIPRLDGKGRTVSVEFMLNTPAVRNMIREGKEHQLYSVLQTGHALGMQTMD
                     QALERLYNAGKISSEKALSYCIDRKEMERMLSFG"
     gene            3436..4647
                     /locus_tag="VFD89_02345"
     CDS             3436..4647
                     /locus_tag="VFD89_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZJ57062.1"
                     /translation="MAAFNYEVVDTAGNVLNGVIAAENKELATQKLKEKGYLIIQIRS
                     QGNRGIRSLSFASRKKVSNGDITIFSRQLATMLNAGIPITRALFTLSEQASNPTLKKA
                     LETIAANVESGMSISNAFGEFPSIFDQMYLGMIRAGEVGGTLGDTLIRLSDQMQKDKL
                     LKDSIKSATIYPIAVVSFSILMLIAMLIFLVPVFQGFFPEDLDIPLATQLIISLSESL
                     RGFWFLWIGLIASIILLIYFYTKTSAGAIMIERIRLRIPAFGPLYHRTVIARFARTFS
                     TLFANGIPVIRALESSGRATGSRLMDSAIGNAIYEIQEGKNIGETFSQEKFFPPIVSQ
                     MVSVGEETGSLPHLLDKIAEFYEDEVTTLSKTITSMIEPVLLIFVGLVVGGMLVALYI
                     PIFTAITQSAA"
     gene            4690..5670
                     /gene="pilM"
                     /locus_tag="VFD89_02350"
     CDS             4690..5670
                     /gene="pilM"
                     /locus_tag="VFD89_02350"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HZJ57063.1"
                     /translation="MNIFSNRSLGVEFDDYEIRTVELYKRAGGIHLSGYHRISLTPDI
                     VKDGKVANPDKMGNYLKRLWTDNRLSKENIVLGLSNQDILIRKITLPMVSKEKMDLLV
                     ANNAQDYIPTPLENAVLDYMILGHSEEDGVEKADVLIVAAASAMVESYYQCFSNLGYG
                     IKDIKVSSLSMADTIDIAGLEDNVVLVDISNNQGNLVIYSGGRPRLARQLRINLVEST
                     GLIPGEIQDHMELMDIVLSTWVQILYNEIRASIQYFQAQEDVQTIDRILLSGCGSRIV
                     EVDKELEKVSGISVQRLDPTEKIRASKTKHLGIQVTDFATCIGLALAGLE"
     gene            5727..6275
                     /locus_tag="VFD89_02355"
     CDS             5727..6275
                     /locus_tag="VFD89_02355"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HZJ57064.1"
                     /translation="MRRYKFYLATFILLLMGVIIILPIVGIYSIKVNTRLHAIKNRNK
                     AIEAYIQENNIPLKALENINRLETLVEKAMDKTQDWGQYIMILGESRPNEVIIDIIKT
                     GLEEDETVLTIDGRAQSQGAVTAYIAELEKSDALESIKCNYVRQIASQGGYSAEFQLI
                     IYMGQVEQFMLDRGGAYEENTN"
     gene            6256..6798
                     /gene="pilO"
                     /locus_tag="VFD89_02360"
     CDS             6256..6798
                     /gene="pilO"
                     /locus_tag="VFD89_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HZJ57065.1"
                     /translation="MKRIQISPRDLVLASVFAVGLLVFIFLAINQWTQFTLAIGDLRL
                     EEERSKTLAMDKEYLIRLGEKEDALLEQMISLEEMMPAEAGTSKLINYLNELLVKGNF
                     HALQLDTHEFIKEDGYYAIPIKIVFEGEYEKIILFLKELRFGSRPFKITELYMDRNDS
                     LPSKVHCEILAHGFVDSKEK"
     gene            6993..>8210
                     /locus_tag="VFD89_02365"
     CDS             6993..>8210
                     /locus_tag="VFD89_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZJ57066.1"
                     /translation="MSNINKKKYLGDYLIEAGIINQNQLNEALDYQKKTLNQGNVGLL
                     GQTLVELGYCTSEDIMLALAMKDGVPFMVLDQDKIDEEAINLLTHDIMERYMAIPVGF
                     DNNRLLVAMLHPTDVIAIDDLRILTGYDIQPIIIPDFQLFGALEQSKSGILGKITFDF
                     KDNETLKSLQDANGMQLYEAIEIEDPSMDEGFEVTDDVNTRPAVQLANTIFNQAVESR
                     ASDVHIEPYEKKTRIRFRIDGVLHEIMNPPKRIHPSLISRIKVMANMDIAERRIPQDG
                     RITLILDSKTVDVRVASLPTAYGEKLTLRLLNRNEKLITIPELGFPDSQLDIFYRIMD
                     LPYGFILVTGPTGSGKSTTLYAALSRLNSVDKNIITIEDPIERRIDGINQTQVNVRAG
                     MTFASGLRSFLRND"
BASE COUNT         2504 a         1581 c         1915 g         2210 t
ORIGIN      
        1 cctatgcata tgggggtatg gacttatagt tttttaatcc ctacagaata gtatgttttg
       61 caccatgcct accgcagaaa atatattata tcccgccatc tggggaattg ggtttccaat
      121 acaagatatg tgacttgtat attttgaaca taagttaatg catagctgct ttcagatttt
      181 tttaggaaag gacgtatgtt tgccatatgg atacaagaat aagaccgggg ttaaagatac
      241 atataaaatc tcgtccaact cactcccatc ttaaattgat tgttttattt gtattaattt
      301 tacttatggc tgtaaatgtc ttaggctgta aaaaatcggc tcccgtcgat ggggaaaatg
      361 ttacagatag tgggggagat gtttcaaata gtccggatgt agacgaatct aagggaaaag
      421 atatgcttcc ccaattagaa agaccccttt tagaatctgg ggatgatgaa acaggacaag
      481 gccccatgct tgaccctttt tcgggttcgc ttacgctgac gggaatcctt acctcaaaat
      541 ttactaagga caatattgcg attatagagt atggaaatac atcctatatc gttaatcagg
      601 atgatataat agctgaatat tggaaagtag gcagtattaa aaacaccggt gtcttattaa
      661 agcatgatga tgtggaactg gtattagagt tctccggtaa ataattctta tatttttaag
      721 acgggggttt tggatgtgag attttctaaa agacttttgt acaagactat atgcctggct
      781 attttgacca gtgtgttggc cacaaccggt atacctccaa gccatagaca gggtatttct
      841 tacggttttg atacggataa ccgggatgca tatactgaat acatagtaaa gcgtggggat
      901 aacctatccg atattgccaa ggaatttgaa acagatatcc caacattgat aagggataat
      961 aatataaaga acaaaaatca tatctatgtg ggtcaaaaac tcaagataaa agatagcgtt
     1021 acagatgata aggatatggt ggacaccgaa gctgataccg acgatgaagc ggcttctttg
     1081 gaacaggatt cccataactc atctggatat gaaagcaggg acgaggattt aatctccctg
     1141 gatatgaggg atgcggatat cagggatgtg atatcatcaa tcgcttatta tttagatacc
     1201 tatgccatct tactggaaga gccgatccgg gttaatttcc aggttgagaa tacaaaaccc
     1261 tataaggcgt tggagcttct gcttcagacc acaggcctta gctatataga ggacgatagt
     1321 ctgttgatca tcggaaaggc cgacaagctt caagaggaat tctacggaca aatggctctc
     1381 acgcgtttta atcttaagca tatatcctcc gaaagcctta aacccctgat tggagagttg
     1441 ggaatccccc tagaaattgt gaccatagac aagaacccca aggctatttg ggtacagggc
     1501 cctccgcaat ccctgtccaa ggtgaaagag ttaattacca tgcttgacaa agcggaaaat
     1561 gccaccgagt tttcaagact gaccagtgtc aagctaaaga atactaccac ggataagctg
     1621 gcatcggtta tcgatgattt ggatatgccg gttaaggtaa tcacgctacc caatatcatg
     1681 ggtaccctct ggctccaggg gactaaggag ggtgtggaaa atgcgaaatc tgtaatttcg
     1741 gccttggata tcgtggaaac gtcctcggat cagtataccc tgttcttatt taagctacgc
     1801 aacatagccg ctaaggatgc agcagaaagg cttgagctct ttggttacga aggggtaaag
     1861 accatcgcct ttcattatcc ggaatttggt aaggatcttt taatcctctg tcccgcaggg
     1921 atgaagacca gggtgacaac ggctctggaa ggactggatc ttaggggact gggggataat
     1981 cccacccata taatgttgcc agtgtactct gcctccggcc ctaatgcgtg ggatgtccta
     2041 gaggctagga ggagcctcct tataatgctt atggatgagc tgaaggacga tagggatctt
     2101 attgatgttt cggatgatat tttgactaag gatggcgagg agtacagggt tttactggta
     2161 aagtctacag ttgaagtcat aaataaggtt agggaaatgg tttcgcttat tgattcgcct
     2221 aatactgatg caccttaagg aaattttata ttctgcgaca ccggagcgca taaggaggac
     2281 aagatttgga gactgaaaaa gacagactgt ttggcggcac agggaaatat tctttggatt
     2341 tagatgccct ctttaagata acggtggaaa ggcatgcatc ggaccttcat cttctctgtg
     2401 gcgcacctcc ccatataagg gtagacggtg atttgatacg cttggatatg cctattatta
     2461 cgcctgattc actaatgaat ctactatatc ccatattaaa caatcgccag attgaaagcc
     2521 tggaaaagga tctagaattg gatttcgcct attcgctgcc ggggatatcc agattcagag
     2581 ggaatgtgat gtttcagcgc ggtacccttg gcgccgcatt tagggtagta ccctacaata
     2641 taccgtccat agattccttg gggttaccca agggggttag ggacctttgt tcactgtccc
     2701 agggcctggt attggtcaca ggaccgacgg ggagcggaaa gtcaaccacc ttagcggcga
     2761 tgctggatta tataggcaac aatagaaggc tcaatatctt gactttggaa gaccccatag
     2821 agtttttaca caagcatggc aactccataa tctcccaaag ggaggtaggg agtgataccc
     2881 actcattttc tacggcacta cgccatgcgt tacgccatga tccggatgta atcctcatag
     2941 gggagatgag ggacttagat agtatatcta tcgcattgac tgcggcggaa acagggcatc
     3001 ttgtattatc taccttacat acccaaaatg cccccctcac cataaatcga atcgttgatg
     3061 ttttcaatgc agatagaagg tcctatatca gacagcaact atctaactcg ctgcaggcta
     3121 tcgtatcaca acaattgatc cctagattgg acggtaaagg aaggactgtt tcggtagaat
     3181 ttatgttaaa taccccggcc gtccgcaata tgatcaggga gggcaaggaa catcagctat
     3241 acagcgtatt acaaacaggc catgccctag gaatgcagac catggatcag gccttggaaa
     3301 gactttacaa cgcaggtaag atttccagtg aaaaagcatt gagctattgt atagacagaa
     3361 aagaaatgga acgaatgctt tcatttggtt agattattat cttttatata tttagaactg
     3421 gaaggaggta atcctttggc agcctttaat tacgaagtgg tagatactgc cgggaatgta
     3481 ctaaacggag taatagccgc agagaacaaa gaattggcaa cccaaaaact aaaggagaag
     3541 ggttacctta tcattcagat aaggagccag ggaaatcggg gaatcagaag cctatccttt
     3601 gcaagcagaa aaaaggtgag caatggggat ataacgatat tcagccgtca attggccaca
     3661 atgctcaatg caggcatacc tataacccgt gctttgttta ccctgagcga gcaggccagt
     3721 aatcccacac ttaagaaagc cttggaaaca atcgccgcga acgtagaaag cggtatgagc
     3781 ataagcaatg cctttggtga gtttccttcc atcttcgatc aaatgtatct tggtatgatt
     3841 agggccgggg aggtaggcgg aactttagga gatacactga taaggctttc tgaccagatg
     3901 caaaaggata aactcctaaa ggacagcata aaatcggcaa ccatataccc aatcgcggtt
     3961 gtatcctttt caatacttat gttaatagct atgcttatat ttctagtgcc ggtatttcag
     4021 gggtttttcc cggaagattt ggatataccc cttgcaacac agttaatcat aagcctgtca
     4081 gaatctctga gagggttttg gttcctttgg attggactta tagcatcgat tattttgctt
     4141 atatactttt ataccaagac ctccgccggc gctataatga ttgaaagaat aagattaagg
     4201 attcctgcat ttggaccctt atatcataga accgttatcg ccagatttgc caggaccttc
     4261 tcgaccctat ttgccaacgg aatacccgta attcgggctt tggaatcttc cggaagggcc
     4321 accggcagca gattaatgga ttcggccata gggaatgcaa tatatgagat acaagagggt
     4381 aaaaacatag gagaaacctt tagccaagaa aagtttttcc cgcctattgt ctcgcagatg
     4441 gtcagtgttg gcgaggaaac gggttccctt cctcatctgc tggataagat agctgaattc
     4501 tatgaagatg aagtaactac tctgagcaaa accataacct ctatgataga gcctgtactt
     4561 ctaatatttg tcggcttggt tgtagggggg atgttagtcg ccctttacat accaatattt
     4621 acggcgatta cacaatctgc cgcttaaggg ccttgtttct tggtaaaaaa ggtggtgaat
     4681 ataagcagta tgaatatttt ttcaaataga tcgttgggtg tcgaattcga tgattatgaa
     4741 atcagaacgg tagaattgta taaaagagcc ggcggtatcc atctctccgg atatcatcgg
     4801 atttcattaa cacctgatat tgtgaaggat ggtaaggtag cgaaccccga taaaatgggc
     4861 aattacttaa aaaggttgtg gactgataac agacttagca aagaaaacat tgtcttaggt
     4921 ctatctaatc aggatatact cattagaaaa attactttac ccatggtctc aaaggaaaag
     4981 atggaccttt tggtggctaa taatgcccag gactatatac caactcccct agagaatgcc
     5041 gtactggatt atatgatcct aggccatagc gaagaagacg gcgtagaaaa ggcagatgtc
     5101 ttgattgttg cagcagcatc ggctatggtt gaaagctatt atcagtgctt tagcaattta
     5161 ggttatggca tcaaggatat taaggtatcc tccctaagta tggccgatac aattgatatc
     5221 gcgggtctag aggacaatgt agtattagtg gatatttcca ataaccaagg aaaccttgta
     5281 atctattcgg gaggaagacc aaggcttgca agacagctgc ggataaacct agtcgaatcc
     5341 acagggctga ttccgggcga aatccaggat catatggagc ttatggatat cgtattatcg
     5401 acatgggtcc agattctata caacgaaata agagcatcga tacaatattt tcaagcgcag
     5461 gaggatgttc aaaccatcga cagaatattg ttgagcggtt gcggcagtcg tatcgtagag
     5521 gtggacaagg agctggagaa ggtttccgga atttcggtac agcgtttgga tcccactgag
     5581 aagataaggg cctcaaagac taaacatctt gggatacagg ttactgattt tgctacctgt
     5641 ataggcttag cccttgccgg tttggagtga taagattgaa cacaattagt ttattgccac
     5701 ctaaaattag actggagcaa aagaagatgc gcagatataa gttttatctg gcaactttta
     5761 ttctcctgct catgggtgtc atcatcatat tacctattgt gggtatatac tcaattaagg
     5821 ttaacaccag gctccatgct atcaaaaatc gcaataaagc cattgaggca tacatccagg
     5881 aaaataatat tccgctaaaa gcactagaaa acatcaacag acttgaaacc cttgtagaaa
     5941 aggctatgga caaaacccaa gattggggtc aatacatcat gatactaggt gaaagtagac
     6001 caaatgaagt aataatagat atcatcaaga cgggcctgga ggaagatgaa acggttctaa
     6061 cgatagacgg ccgtgcccag agccaggggg ctgtaaccgc ctatatagcg gagctggaaa
     6121 aatcagatgc cctagagagc atcaaatgta attatgtaag gcaaattgca tctcaagggg
     6181 gatattctgc agaatttcaa ttgatcatct atatggggca agtagaacaa tttatgctgg
     6241 atcggggagg ggcctatgaa gagaatacaa attagtccca gggatttggt gctggcgtcc
     6301 gtctttgctg tgggactttt ggtgttcatt tttctggcaa taaatcaatg gactcaattt
     6361 acgttggcca ttggtgatct aaggttggaa gaggagagat ctaaaaccct ggctatggac
     6421 aaggaatatc tcattcggtt aggagaaaaa gaagatgccc tgctggagca gatgatatcc
     6481 ctggaagaaa tgatgcccgc ggaagctgga acctcaaaac tgatcaacta cctcaatgaa
     6541 ttattagtga agggaaattt tcacgccctt cagctggata cccacgaatt tattaaggaa
     6601 gacggatatt atgctattcc tataaaaatc gtattcgaag gtgaatatga aaaaattata
     6661 ctattcctaa aagaacttag atttggcagt aggcccttta agataactga actctatatg
     6721 gatagaaacg atagcctgcc ttccaaggtt cattgcgaga ttttggccca tggttttgta
     6781 gacagcaagg aaaagtaatt ctgcgctatc tgaacggtta ctacatgttt tacgacaaat
     6841 attctacttt aatatacaag tgtcgtaaac attatccaaa attcatatgt tacgaaagat
     6901 acattgtttc atcgcaaaat cggatagttt agctgggttt acgatgaaaa gtctttatcg
     6961 aaacatatat gttagctcat ggaaggtgga acatgtcgaa tattaacaag aaaaaatatc
     7021 taggtgacta cttaattgag gctggtatca taaatcagaa tcagctgaac gaagccctgg
     7081 actaccaaaa gaaaactcta aatcagggaa atgtggggtt gttaggtcag accctggtag
     7141 agcttggcta ttgtaccagt gaggatatca tgttggccct tgctatgaag gatggagttc
     7201 cctttatggt attggatcag gacaagatag atgaagaggc cattaacctt ctaacccatg
     7261 atattatgga gcgctatatg gctatccctg ttgggtttga taataatagg ctcttggtgg
     7321 cgatgttaca tcccaccgat gtcattgcga tagatgacct gcgcatcctg acagggtatg
     7381 atatacagcc catcatcatt ccggattttc aattatttgg tgccttggag caatctaaat
     7441 ccgggatttt aggaaagatc acctttgatt ttaaagataa cgaaacccta aaatcgttac
     7501 aagatgccaa tggtatgcaa ctatatgaag cgattgagat tgaagatcct tcaatggacg
     7561 agggttttga agtcacagac gatgtaaaca ccagacctgc ggtacagctg gctaacacca
     7621 tatttaacca ggctgtagag tcaagggcta gtgatgtaca tatagaacca tacgagaaaa
     7681 agactaggat tcggtttaga atcgatgggg tgttgcatga gattatgaat cccccgaaaa
     7741 gaatccatcc ttcccttata tccaggataa aggtaatggc caatatggat atagcggagc
     7801 gcagaattcc ccaagacggg agaataactc tgattttaga ttccaagacc gttgatgtca
     7861 gggtagcttc gctccctacc gcttatgggg aaaagttgac cctaaggctt cttaatcgca
     7921 atgaaaagct tatcaccatc cctgagctgg gctttcccga tagccagttg gatatattct
     7981 acaggataat ggatcttccc tatgggttta tcctagtaac gggccccacc ggtagcggca
     8041 agagcactac cctctacgca gctctgtcta ggcttaattc ggtggataag aacatcataa
     8101 ccatagagga ccctatagaa agacgaatag acggcattaa ccaaacccag gtaaatgtaa
     8161 gagcagggat gacctttgca tcgggactcc gttcgttttt gcgcaacgat
//
LOCUS       DASRNX010000059         9652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_170904,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000059 DASRNX010000000
VERSION     DASRNX010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170904"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFD89_02370"
     CDS             complement(<1..398)
                     /locus_tag="VFD89_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57067.1"
                     /translation="MGSYREDYFKAMKFEYPGKIPTEVSLLPATWAKYEESLEDIVLR
                     YPAIFRGYEKGSYDYNAKPETYREGEHVDVWGCVWSNIHEGMEAIVTHHPVPTRQSVH
                     LLKLPEKDAAIPHGFMYLRLADLRGFEELM"
     gene            complement(617..1216)
                     /locus_tag="VFD89_02375"
     CDS             complement(617..1216)
                     /locus_tag="VFD89_02375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZJ57068.1"
                     /translation="MLFDTIAPIYALFFNYQVRYYRKVLDRMKDWIDVDEHNKILDFG
                     CGTGALSYALQERGLEVVGVDTSAGMLRRAKNKLQDTGVKLIKIEPGEDLPFDDDSFD
                     LAISSFVIHGLKPEERRKVYGELSRVSKADIVFHDYNRNRSIITDIAEYLERGDYFNF
                     IKVAEGEMKEFFDSVRIVDVDKRAAWYIIKKARDGCDKG"
     gene            complement(1782..3974)
                     /locus_tag="VFD89_02380"
     CDS             complement(1782..3974)
                     /locus_tag="VFD89_02380"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZJ57069.1"
                     /translation="MVSITVYLVGLGAILSLIFAYYNFQKGTKMEHGTPVMEDIADAI
                     REGADAFLRREFAISIPIIVGISVLFGVIFTPWAGMAFLIGAAMSSFAGLVGMKAAVI
                     YNERVANEARLGVERKDPSALGKALNVAFRGGSVMGLSVGGFAMLGLLVVYLVVGIML
                     EYGNLENLVETTSWIGLKFVLFPQVLACYSLGCSSIAVFNRLGGGIYTKGADMGADLV
                     GKTELGLPEDDPRNPAVIADCVGDNVGDVNGNGSDLLESTIGSIVASSVLPTAIHTAA
                     INAKTPISTELITRMMQFPFAFVGVGLLACILGIAYVLIRKPSNKLSRELNISLWSSA
                     TIIAIATLVLSYYFFAGFDYTGVDFRFGWISPWLSAITGIIAGILLGIIAEIYTSEQY
                     KETKNVAHLAKEGPALVIIGGTSTGWRSCLPACVTLGGAIIASSYFSGLYGSAISAVG
                     MLSFVVATVTVDTYGPISDNAGGIAEMAQLDKEVRNITDSLDSQGNTTAAIGKGFAIG
                     SGVLAALAMMMSFASGGPDGGELNILNYLSLGGAFFGVGLMHWLSGVMLNSVCRNAEQ
                     MVEEVRRQMRENPGILTGEMKPDYNICIDICTKSAIAEMKKPVLVSLLAPIVGGFIFG
                     PMFVGGFLLGTILDSCVMATSTANSGGTWDNAKKYIKSLEAELVEQFGEKGFDEIHNA
                     SIIGDTVGDGYKDVVGPNQDIMIKMMATVSVIMLPVMNAFNIISMLIK"
     gene            complement(4118..5500)
                     /locus_tag="VFD89_02385"
     CDS             complement(4118..5500)
                     /locus_tag="VFD89_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZJ57070.1"
                     /translation="MENELINNENPLGTEPVGGLLQKYAIPSIVAMLVGALYNIVDQF
                     FIGQKIGELGNAATNVAFPLSTACVAIALLLGVGGAAAFNLTMGEGDKEKAVYYIGNA
                     TVMLFILGLALSIITQIFLTPMLKFFGSPDNVLGYARVYTRITSLGFPFLIVSNGGGH
                     LIRADGSPSYAMFANLLGAVINTVLDPIFIFVFNMDMAGAALATVIGQIFSFLMAARY
                     FRNFKSVHIQKHHFVPVWKYIKRITTLGAAPSFNQVAMMIVQIIMNKSLTHYGAMSVY
                     GKSIPLAAAGIINKVAMIFFSVIIGISQGMQPIASFNYGARKFDRVKGVYKLAIRSGF
                     VISTVAFLSFQVIPRQIISIFGSGSKEYFDFAVSYFRIYMLFTFINCVQPVSSNLFTA
                     IGKPKKGIFLSLTRQIIFLLPFILILPLLMGIDGIMYAGPIADLAAAIVSGIMVIVEF
                     RNMGKISLAV"
     gene            complement(6031..7434)
                     /locus_tag="VFD89_02390"
     CDS             complement(6031..7434)
                     /locus_tag="VFD89_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZJ57071.1"
                     /translation="MKTTKPNSLTEGSISKALFRLAIPIMGTSFVQMAYNMTDMIWVG
                     KIGSRAVASVGTAGFYTWLAMAFILIPKIGAEVGVAQSVGRKDMKEARTYIRHSIQMS
                     VVLALFYAMILLIFRRPLIGFFNLGEKDIVDDAIIYLSIVSSGMVFFCINPVFSAVFN
                     GYGESKIPFIVNSLGLVINMVLDPMMILGIGPFPKLGVAGAALATILAQAIVTITFII
                     TARRKSVLFSELSLFGAPDMAHIREITKLGFPVALQSGLFTSFSMMIARIIARWGPIP
                     IAVEKVGSQIEAISWMTSGGFQTAMSAFVGQNYGAKKWDRVSKGYTVGMIIISGIGLI
                     ATTLLVFFGRPLFTVFIKEEETIVQGVAYLKILGLSQLFMCIESTTAGAFNGLGKTVP
                     PSVVGITLNGLRIPIALILSSTTLGLNGVWWAISITSVLKGTILMTWYIIMQKRNPKT
                     KDLGEGDLALKQIEESI"
     gene            complement(7538..8572)
                     /locus_tag="VFD89_02395"
     CDS             complement(7538..8572)
                     /locus_tag="VFD89_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HZJ57072.1"
                     /translation="MPFTIVRNDITKMNVDAIVNAANTQLLMGGGVCGAIFEGAGADK
                     LQKACRALSPIKTGEAVITLGFDLPAKYIIHTAGPVYKDGKQGEGDLLRASYINSLRL
                     AAREDMESIAFPLISSGVYGYPKAEAFQIAAKAIKGFVEYHDMDVYLVVFDRESLDIS
                     RELMGEIEAFIDENYVEDYRDLRLQRRLLDIEGKPTDVAESRPLMLEEYFVTEKGIEP
                     LHRKIEDVIGDLDEPFAATLFRMIDHKGFTDVEVYKKANLDRRLFSKMRSNAGYMPSK
                     RTAIALAIALELTLEETHDLLRRAGYTLSRSQKFDVIIEYFIIQGSYDIFKINEILFS
                     YDQPLLGGVG"
     gene            complement(8590..8916)
                     /locus_tag="VFD89_02400"
     CDS             complement(8590..8916)
                     /locus_tag="VFD89_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HZJ57073.1"
                     /translation="MGISEYQDKAMRTKNQFTSIEGQILNASLGLAGEVGECVDHIKK
                     GIFHGHDLDKDYIKKELGDIAWYIALMSDALGLSMEDVYKENIMKLEKRYPNGFDKGH
                     SLNRDE"
     gene            complement(8935..9414)
                     /locus_tag="VFD89_02405"
                     /pseudo
     CDS             complement(8935..9414)
                     /locus_tag="VFD89_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009795348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
BASE COUNT         2715 a         2429 c         1867 g         2641 t
ORIGIN      
        1 atcataagct cttcaaaacc ccggagatct gccaacctca gatacatgaa tccgtgggga
       61 attgcggcgt ctttttcagg cagctttaag aggtgtactg attgtctggt gggaacgggg
      121 tgatgggtta caatggcctc cataccctca tggatgttac tccatacaca tccccaaaca
      181 tccacgtgct ccccctcccg gtaggtttcg ggtttggcgt tataatcata gctgcccttt
      241 tcgtatcctc tgaaaatagc agggtatctt aatacgatat cttcaaggga ctcctcgtat
      301 ttggcccatg ttgcgggcaa tagggatacc tctgtgggaa ttttacctgg atattcaaat
      361 ttcatggcct taaaataatc ttccctataa gaccccataa ccgccctccc cctattatgt
      421 tcctaaaccc tctatagttt tagtgcttta cataaataat acaaaactaa atttatcatc
      481 atgaagctgc ataatctcat agcgattctc attccgataa gttttacagt cggaaccgct
      541 tatctgtaat acacaacaat tataccatac cggaggggag agattcaatc aaaaccggaa
      601 ttatgctgat tgccaattaa cccttatcac acccatccct tgcttttttg attatgtacc
      661 atgcagccct tttgtctaca tcaactatcc ttacgctatc aaagaactcc ttcatctccc
      721 cttcggcaac cttgataaag ttaaagtaat cccctctttc caggtactcg gctatatcgg
      781 ttattatgga cctattccta ttataatcat gaaaaacgat gtcggctttg gataccctgg
      841 acagttcccc atataccttc cttctctctt ccggcttcag cccgtgtatt acaaaggatg
      901 agattgccag atcaaagcta tcgtcgtcaa agggcaaatc ctcgccgggc tctattttta
      961 ttaattttac cccggtatcc tgcaatttgt ttttcgctct gcggagcata ccggcagatg
     1021 tgtccacacc aaccacctct aagccccgct cttgcagggc ataggatagc gccccggtcc
     1081 cgcaaccgaa atccagtatt ttattatgct catctacatc gatccaatct ttcattctgt
     1141 ccaagacttt tctgtaatat ctaacttggt aattgaagaa aagcgcgtat atgggagcaa
     1201 tagtgtcaaa tagcatgggc cacctccgcc atatctttta tcatattgtg cacatcatcc
     1261 atatatatac ccttatgggg cctctagaaa cttcccacgg tccattttaa attcccccca
     1321 gcctgggcca gggaggttgc catggcctgt ccggagtagt aagataattg tatgaaggga
     1381 ttcataccag aaataaatca gacttaaata tattcatctt caatagctat ttcctaatcg
     1441 cctcaaaaca ttacacatcg tatatgaacc ccaacttcag agttattaat tcatgttaaa
     1501 taaatatggc tgcaagcgct cctccaagga gttgttgcag ccatactatt tattacatat
     1561 actattatat atagataacc aatacggaaa ctaagtttac cagcatcata attgcattga
     1621 ttttgtgcaa tttcataata ataaatttta tagttgaaac tggttatcta tacatccgta
     1681 accgcataca caaaacaaag tatcaaaggc gattacatca ggtatctttc aacgatgcag
     1741 gggatcatta tccaccatat cgttgggttt tcacccaaat cctatttaat caacatggaa
     1801 attatattaa aggcgttcat aaccggcagc ataataactg aaaccgtagc catcatcttg
     1861 atcatgatgt cttggttagg accgacaacg tccttgtatc cgtcaccaac tgtatcgccg
     1921 atgatgctgg cgttatgaat ttcatcaaag cccttttcac caaattgctc cactaattct
     1981 gcttccagag attttatata ttttttagca ttatcccatg taccgccgct gtttgcagtt
     2041 gaagtcgcca ttacacagct gtcaagtata gtaccaagca ggaatccacc aacaaacata
     2101 ggcccaaaga taaagcctcc tacgattggt gccagcaagg atactagtac aggctttttc
     2161 atctctgcga tagcactctt tgtacatata tcgatacaaa tattgtaatc aggcttcatt
     2221 tcaccggtca agatacccgg gttttctcgc atctgcctac gtacctcttc aaccatctgc
     2281 tccgcgttac ggcatacgga gttgagcatt acacccgaga gccagtgcat aaggcctacg
     2341 ccaaagaacg cgccccctag ggacaggtag tttaagatat taagttcgcc gccatcaggt
     2401 ccgccactgg caaaggacat catcatagcc aatgccgcaa gaacaccgga accgattgca
     2461 aaacccttac caatagccgc agtggtgtta ccctgagagt ctaggctgtc ggtgatgttt
     2521 ctaacttcct tatccaattg agccatctct gcaataccac cagcattatc tgaaatagga
     2581 ccgtaggtat caacggtaac ggtggccaca acgaatgaga gcataccgac tgccgagata
     2641 gccgaaccgt agagccctga aaaatagctt gaagcaataa ttgctccgcc caaagtcaca
     2701 catgcaggca ggcagcttct ccagccggtg ctggttccgc cgataataac cagtgcggga
     2761 ccttccttgg ccagatgggc aacatttttt gtctctttgt attgttcact cgtatatatt
     2821 tccgcaataa tacccagtaa gatacccgcg atgattcccg ttatagcact cagccaaggg
     2881 gatatccatc cgaaacggaa atcaacaccc gtatagtcaa aaccggcaaa gaaataatag
     2941 ctcagtacaa gggtagcgat tgcaatgatg gtcgccgagc tccataggga tatattaagt
     3001 tccctgctga gcttattgct gggctttctg ataagaacat aggcaatgcc caagatacag
     3061 gcgagtaaac cgactcccac aaaagcgaat gggaattgca tcatgcgtgt gattaattca
     3121 gtgctgatag gagtcttagc attaattgct gcggtgtgaa tagcagtcgg caatacggag
     3181 gatgccacaa tggagccaat ggtactttcc aacaagtccg aaccattacc gtttacgtca
     3241 ccaacgttgt caccaacaca atccgctata actgcggggt ttcgtgggtc gtcttcaggc
     3301 aaccccagct cagtcttacc taccaaatcc gcgcccatat ccgcaccctt ggtgtatata
     3361 ccgccaccaa gtcggttaaa cacggcgatt gaagagcaac ccagagaata acacgctaaa
     3421 acctggggaa agagtacaaa tttaaggcca atccaactag tagtttcgac aagattctcc
     3481 aaattgccat actcaagcat tataccaaca actaagtaca ccacaagaag tcccagcata
     3541 gcaaatccgc caacggataa acccattacc gatccacccc ggaaagcaac gttcagcgcc
     3601 ttccccaagg ctgatggatc ctttctttct acgcctagac gggcttcgtt agcgacgcgc
     3661 tcgttgtata tgaccgcagc tttcatacca accaaaccgg caaaggagct cattgctgca
     3721 cctattaaaa atgccatccc ggcccagggc gtaaatatga caccgaataa taccgatatc
     3781 ccaacgatga ttggaatact aatggcaaat tctcttctca agaaagcatc cgcgccttct
     3841 ctaatcgcat ccgcaatgtc ctccattacg ggtgttccat gctccatttt cgttcctttt
     3901 tggaagttat aataggcaaa gatcagagag agaatagccc ccaaacccac aagataaacc
     3961 gtaattgata ccattgaacc acgtcctcct agatcgacaa tatttttacc cacaagaaat
     4021 attatatcaa tgatatagat aaaaggcaac tatatatgta aatggtatat atgtctaata
     4081 tacgccgcag aatagagtat cggaccgatt ggacaggcta taccgccagt gaaatttttc
     4141 ccatattccg aaactccact atcaccataa taccagaaac gatggcggca gcaaggtccg
     4201 ctatgggccc tgcgtacatg atcccatcaa tgcccatcag gagcggcaaa ataagtataa
     4261 aaggtaaaag gaagatgatc tgtctggtta gggatagaaa gatacccttc ttcggtttac
     4321 ctatggcggt gaataggttg gaggatacgg gttgtacaca atttataaag gtaaatagca
     4381 tatatatacg aaagtaggat acagcaaagt caaaatattc tttggagccg gacccgaata
     4441 tggatattat ctgtctggga ataacctgga aggataaaaa tgcaacagtt gagataacaa
     4501 agccggaacg aatagccagc ttgtagacac cctttaccct atcaaatttc ctggctccat
     4561 agttaaagct ggcgatgggt tgcatccctt gagaaatccc gatgataacg gaaaagaata
     4621 tcatagctac cttatttatg attccggcgg cagccaaggg gatggattta ccgtagacgg
     4681 acatcgcacc ataatgggta agggacttgt tcatgattat ctggacaatc atcatggcca
     4741 cctggttgaa agacggagcc gctcccaaag tggtaatccg ctttatatat ttccagaccg
     4801 gtacgaagtg atgcttttgg atatgtaccg atttaaagtt tcgaaaatac ctagcagcca
     4861 tcaaaaatga gaatatttga ccgattaccg tggccaaggc agccccggcc atatccatat
     4921 taaatacaaa tataaagata ggatctaaga cggtgttaat tacagcgccc aataggttgg
     4981 caaacatggc ataactgggg cttccgtcag cccttatcag atgtccgcct ccatttgaaa
     5041 caatgagaaa gggaaagccc aaagaggtga tccgggtata gaccctggca tagcccagca
     5101 cattatccgg tgaaccgaaa aactttagca tcggggttaa aaatatctgt gtgataatgc
     5161 tcagcgccag cccaaggata aataacatca ccgtggcgtt tcctatatag taaacagctt
     5221 tttccttatc cccctcgccc attgtaaggt tgaaggcggc agcaccgccg acccccagca
     5281 acagtgctat agctacgcag gctgtgctca ggggaaaggc tacattggta gcggcgtttc
     5341 ccagctctcc gatcttttgg ccgataaaga attggtccac gatattatag agcgccccaa
     5401 caagcatagc tactatactg ggtatcgcat atttttgaag cagtccccct acaggttccg
     5461 ttccaagggg attttcgttg tttataagtt cattttccat agctatctcc attcattcca
     5521 ctattgtatg ctttcgttat gggtgcacat tctactcaag tagttaggga tatgtacccg
     5581 attcgattat atgtgtggta aaattatctt tcattagaat aacctgtcgt gctatatatg
     5641 ttacgaaaga gacctttcat cgtaacatcg gttaaattat ccgattttac gttaaaataa
     5701 tgtgtttttc gtaacatacg aattaaggat aatgttttac gacacctgtg taccaatgta
     5761 gaatgtttgt cgtaaaacat atagttaaac gtgtcggcga tgactatcct atgctctttc
     5821 cgacattgat ttggagaaaa caaaattcat ctgcagatga taaattttat atagataaac
     5881 aactccaact atagaatcta tcaccatgaa attgcacaga accgttgtaa ttatcatgtc
     5941 gaacaattta gttgacctat ctttatctgt gatcagagtt gtttatctgc agcatagcat
     6001 attgggctag tttagcagaa tcggcgatta tcatatggac tcctctattt gctttaatgc
     6061 cagatctccc tcacccaaat ccttggtctt ggggtttctt ttctgcatga taatatacca
     6121 ggtcattaaa attgtgccct ttaatacgct ggtgatgctg atcgcccacc atacgccgtt
     6181 taaccccaat gttgtggaag atagaatgag cgcaatgggt atcctcagcc cgtttaatgt
     6241 tatacccacc accgatgggg gtacggtttt cccaagaccg ttaaaagcgc cagccgtcgt
     6301 agactctatg cacataaata gctgagacag acccaatatc ttaaggtagg ctaccccctg
     6361 tactattgtt tcctcttcct ttataaacac cgtgaacaag ggtctgccaa aaaacaccaa
     6421 aagggtggta gcaatcaatc cgataccgga tataattatc atccccaccg tgtatccctt
     6481 ggaaacccta tcccactttt tggcaccata gttttgtccc acgaaggcgc tcatagcggt
     6541 ttgaaatccc ccgctggtca tccaggatat cgcctctatc tgtgacccca ccttctcaac
     6601 ggcaatgggt ataggtcccc accttgctat aatgcgagct atcatcatgg aaaagctggt
     6661 aaatagtccg ctttgaagtg cgacaggaaa tcccagcttt gtaatctccc ttatatgagc
     6721 catatcgggg gccccaaaaa gtgacagctc cgaaaatagc accgattttc gtctggcagt
     6781 aattataaag gttatggtta caatcgcctg ggccagtatc gtagccagtg cggcccccgc
     6841 tacccccaat tttggaaacg gaccaatccc tagaatcatc atgggatcta aaaccatatt
     6901 gataacaagc cccaaggaat ttactataaa ggggatttta ctctccccat agccgttgaa
     6961 gacagccgaa aagacgggat taatacagaa aaataccatc ccggacgaaa cgatgctcaa
     7021 ataaattatg gcatcatcta caatgtcctt ttctcccaga ttaaagaatc cgataagggg
     7081 tcttctgaag attaggagta tcattgcata aaaaagtgca aggacaacgg acatctgaat
     7141 gctgtgcctt atatatgtcc tggcctcttt catatccttc ctgccgacgg attgggcaac
     7201 ccctacctct gcccctattt tgggtatcaa aataaaggcc atagccaacc aagtataaaa
     7261 accggcagtc cccaccgagg caactgccct tgatcctatt ttgcccaccc agatcatatc
     7321 cgtcatatta tatgccattt gaacgaagga agtccccatg atggggatag cgagccgaaa
     7381 gagagccttt gatatgcttc cctctgtcag gctgttgggt tttgtcgttt tcatgtctgc
     7441 ctcccgccgg tataagtccc cctgtattgt ttaccttgac atagtagcag aaagcagcct
     7501 aaaaatcaat gcagggatga aatgccaaaa agccccttca tcctaccccg cccaataagg
     7561 gctggtcata gctgaagagg atctcgttga tcttgaatat atcgtagctt ccttgaatta
     7621 taaaatactc tataattaca tcaaattttt ggcttctaga cagggtataa cccgcccttc
     7681 tcaacagatc atgggtctct tccagggtga gctccaaggc aatggccagg gctatggctg
     7741 tcctcttgct gggcatatag cctgcgttac ttcgcatctt tgaaaacagc cttctgtcca
     7801 aattggcctt cttgtagacc tccacatcag taaagccctt atgatcgatc attcgaaaca
     7861 aggtcgcagc aaagggctcg tccaaatccc ctataacgtc ctctattttt ctatgcagcg
     7921 gttctatacc cttttctgtt acgaaatatt cctctaacat aagcggccgg gattctgcta
     7981 catctgtcgg tttcccttca atatccaaca gccttcgctg taatctcaga tccctataat
     8041 cttcaacata attttcatca ataaaggcct cgatttcacc catgagctcc cgacttatat
     8101 ccaaggactc cctgtcaaac accaccaagt atacatccat atcatggtac tctacaaatc
     8161 ccttgatggc tttagcggcg atctgaaatg cctccgcttt agggtagcca taaaccccgc
     8221 ttgagatcag gggaaaagct atgctttcca tatcttcccg cgcagcaagt cttagagagt
     8281 ttatatagct ggcccttaat aggtccccct cgccctgctt cccatcttta taaacagggc
     8341 ctgccgtgtg gataatatac ttggcaggca gatcaaaacc cagcgtaatt accgcctccc
     8401 cggtcttaat cggtgaaagg gcccggcatg ccttttgcag cttatcagcg cctgcgcctt
     8461 cgaatatagc accgcatacc ccgccgccca tcaacagctg agtgttggcg gcgttcacta
     8521 tcgcatccac attcatcttt gttatatcat ttcggacaat ggtaaatggc ataagacccc
     8581 acctccctcc tattcatccc ggtttagact atgcccctta tcaaaaccgt tcgggtatct
     8641 tttttccagc ttcatgatgt tctccttata tacgtcctcc atactaagcc ccaacgcatc
     8701 ggacatgaga gcgatatacc aggcgatatc ccccagttcc ttttttatat agtccttgtc
     8761 caagtcatgc ccatggaaga tccccttctt tatgtgatct acacattcgc ctacctcccc
     8821 ggcaagcccc aaggaggcat ttagaatctg accttctatc gatgtaaatt gattctttgt
     8881 tcgcatggcc ttgtcttggt attcggatat gcccatgctg tcttctggcc atattaaatg
     8941 ttcgttgaga atttctataa agtcctgtag accctttgca tcctcctcat caaaccgggc
     9001 ctttacgggg ctgtcgatat caagcacccc aatgagcctg ccttcctcta ttatgggaag
     9061 cactatctcc gattgggagg cggcatcgca tgcgatatgg cccggaaact catgtacatc
     9121 cttaactatc tgtatctttc cggtttctgc cgcagtaccg cataccccct tgcccatctc
     9181 aatgcgaacg cacgccggtt taccctgaaa gggtcccaac accaattctc cgtccttata
     9241 gagataaaaa ccggcccaat taatgttatc catggttaga tataataagg aagaagcgtt
     9301 ggacatcccc gccagccaat ccggctcccc ttccagtaat ccttccagat agccgtttaa
     9361 ttccctataa aattccgttt tatcttcccc ggatattgct tctatttcaa acatgatgat
     9421 attccccctg ccgtttttta aatgccgggc tttcccatat tatctcacgt ttttgtttgc
     9481 gtgacaagaa aggatgatgg gcccatacaa tgccccgatg tatccatcga ttccgatatt
     9541 cgttcatcaa tgactaattg tcttcattga acgatcatac agcagaccga tgcaacagat
     9601 tatcttataa aatttaaagg aattcagaac gatttgtaga atagtatata ta
//
LOCUS       DASRNX010000060         5209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_490019,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000060 DASRNX010000000
VERSION     DASRNX010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490019"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFD89_02410"
     CDS             <1..363
                     /locus_tag="VFD89_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HZJ57074.1"
                     /translation="FYDIKLMDPVLHRKYTGKTNELILNNLKALCADRKIAEKILIRI
                     PCIPNVNDSPEQIVEIANFVLNLGIQRIELMPYNEMAPSKYEWVDRTYPLGDVKGRSK
                     DYYRDLNRLVAEMGIRVI"
     gene            408..2822
                     /locus_tag="VFD89_02415"
     CDS             408..2822
                     /locus_tag="VFD89_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HZJ57075.1"
                     /translation="MESILEHGERQAQEMESLREMTPRIKTHYNTIKERMEQRQLAWG
                     GDLSVLDEETWGYRTNIRRAMAFERVMNEMPIAIEDYDLIAGRSLLNGQIVRCNLPIF
                     LKPGELGECNIHISHKCPDYETLLSQGMIGILTRLEEQEKTLPSIQDPDEREEKKAFI
                     EGVRIEAEAIVKMANRYSELAVSKAEAETDDKRKAEWLEMARICKKVPLYPAESFREA
                     IQSIWFVNYTFSHTMTYIPIGRIDYLLYPYFLMDFENGRIRVEEAQELVDSFCLQVND
                     RAQLHPKNYLYTDQTSIPGSPRQSVMLYNNGFVKKGDGDASDAINHWGQNILISGLNP
                     DGSDSTSILTYLFLNAHEKISMTSPVLTVRMHKNSPKPLLDRVAQVLKTGGGMPFINN
                     DDVIIDAYEKLGVPREDACNYANSNCWETLIQGMSNQEMIRGINFLYLLELALNGGKP
                     FVNKANPMVMVDYSKVDPMSLSPYVGPSNPVVDGVDTGDASQITDFEKLMDLWRIQMD
                     YMLKHSMDHVTRIIFSEGTHGKLGNNPLLSALTRDCIENIKDLTHMGARYNLWHILAE
                     AVSNAADAAMAIKRFVFEEKLFTLPQLVEILKNDWAGEETLRNKFLAGAPKWGNGDAE
                     VDNIAAEMTDYFLERVKYHAKSYPQIIFSPSIATFSWVISIGKRIGASADGRNAKELI
                     AANLSPAPGRDTAGPTAAINSYMKIDTSTMAAGAPIDLRVSMNGLEGEEGTKRISALV
                     ETFLELGGNMMTLTLTSAEELRQAIEHPENYSGLRVRMGGWSAYFTLLSKESQKLHLH
                     RVEHNL"
     gene            3008..3967
                     /locus_tag="VFD89_02420"
     CDS             3008..3967
                     /locus_tag="VFD89_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZJ57076.1"
                     /translation="MMLIDMPLEELREYRGINPRPHDFDEYWGSALKEMRALDPKVEM
                     VPAKFQTPHAECFHLYFTGVGGARIHAKYLRPKGVTKPHPAVLMFHGYTGNSGSWSDK
                     LAYVGNGFAVAALDCRGQGGLSEDIGGVKGNTFRGHIIRGLDDSPKRLLFREIFLDTA
                     QLAKIVMDMPEVDEDRVGAMGGSQGGGLTLACASLEPKIKRVAPVYPFLSDYKRVWEM
                     DLAKGAYQEITDYFRLFDPTHAREEEVFTTLGYIDVQHLTERIEGEVMMAVGLMDTIC
                     PPSSQFAAYNKIISPKETIIYPDFGHEGLPGFDDMTFQFMMGL"
     gene            4367..>5209
                     /locus_tag="VFD89_02425"
     CDS             4367..>5209
                     /locus_tag="VFD89_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HZJ57077.1"
                     /translation="MSKRMTTRICILALVTLMLLGQVFTFTSHSNAASSTVTQTLRQG
                     SRGSQVKILQQKLNSLGFNAGDVDGSFGPKTRAAVLAFQKAYELARDGVVGPATRAKL
                     NPGKPKDSKPQDPKPADPKPETSPAKDIPQVTQTLKRGSKGTQVKYLQQRLNALGFNV
                     GEVDGSFGPKTLAGVHAFQKANGLAVDGSVGPATRAKLNAAIKPPKPEEPKPEKPKPE
                     EPKPEKPKPKEPKPEKPKPETPPAKEIPPVTQTLRRGDKGTQVKNLQEKLNYLGFDVG
                     EVDGS"
BASE COUNT         1577 a         1091 c         1289 g         1252 t
ORIGIN      
        1 ttttatgata taaagctgat ggatccggtt cttcaccgaa aatacacggg aaagaccaat
       61 gaactaatcc ttaacaactt gaaagcctta tgtgcggata ggaagatagc agaaaaaatc
      121 ctaatccgca tcccatgtat acccaatgtt aacgattctc cggagcaaat cgttgaaata
      181 gcgaactttg tcctaaatct tgggattcaa agaattgagc tgatgcccta taacgagatg
      241 gccccctcca aatacgaatg ggtggacagg acatacccct taggggatgt gaagggccgt
      301 agcaaggact attatcggga cttaaacaga ttggtcgccg aaatgggcat aagagtaatt
      361 taatcaaatc aatatatata ggtaaaaagt gagggggcta caaaacaatg gaaagcattc
      421 tagaacatgg cgaaagacag gcgcaggaaa tggaatccct acgggagatg acaccgagga
      481 tcaagaccca ttacaacacc ataaaggaaa gaatggaaca aagacaatta gcttggggag
      541 gcgatctttc ggtcctggat gaagaaacct ggggctatag gacaaatatt cgaagggcta
      601 tggcatttga aagggttatg aatgaaatgc ccattgccat agaagactac gacctcatcg
      661 caggcaggag tcttttaaac ggacagatag ttcgctgcaa ccttccaatc tttttaaagc
      721 ccggtgaact gggggagtgt aatatccaca tatcccataa atgccctgat tatgagacct
      781 tgctaagcca ggggatgatc ggtatattaa cgaggcttga ggaacaggaa aaaaccctgc
      841 ctagcattca agacccggat gagcgcgaag aaaagaaggc ctttattgaa ggcgttagga
      901 tagaggccga agcgatagtt aaaatggcaa atcgctacag tgaactggcc gtttccaaag
      961 cagaagctga gacggatgac aaacgaaaag cggaatggct ggaaatggca cgtatctgca
     1021 aaaaggttcc cctatacccg gcggaatcct ttagggaggc catccaatcc atatggtttg
     1081 taaactatac tttttcccac accatgacct atattcccat aggccgaatt gattatctcc
     1141 tctatccgta ctttcttatg gattttgaaa acgggaggat aagggttgag gaggctcaag
     1201 aattagtcga ttccttttgt cttcaggtaa atgacagggc gcaacttcat ccaaaaaatt
     1261 atctgtatac cgatcagact tcaatccccg gctccccaag acagagcgtc atgctctata
     1321 ataacggttt tgtgaaaaaa ggggacggcg atgcctcgga tgccatcaac cattggggcc
     1381 aaaacatttt aatcagcggc ttaaaccccg acggaagcga tagcaccagt attttgacct
     1441 accttttttt aaacgcccat gaaaagatct ccatgacaag tccggtattg acagtacgca
     1501 tgcataaaaa cagtccgaaa cccttacttg atcgtgtcgc ccaggttcta aagaccggcg
     1561 gggggatgcc ctttataaac aacgatgatg tgattataga tgcctatgaa aagcttgggg
     1621 tgcccaggga ggatgcatgc aattacgcaa actccaattg ctgggagaca ctgattcagg
     1681 ggatgtccaa tcaggagatg atccggggaa taaactttct ttatctgctg gaactggctc
     1741 taaacggagg aaaacccttt gtcaacaagg ccaatcccat ggtaatggta gattacagca
     1801 aggtggatcc tatgagcctt tcaccctatg tagggccgtc caatcccgta gtcgacggtg
     1861 ttgatacggg ggatgcatcc caaattaccg actttgaaaa gctaatggat ctgtggcgca
     1921 tacagatgga ttatatgtta aaacattcga tggaccatgt tactaggatt atctttagcg
     1981 aaggcaccca tggtaaactt gggaataacc ctctgctctc agccctcaca agggactgca
     2041 ttgaaaacat taaggatcta acccatatgg gcgcccgtta taatctatgg catattttag
     2101 ctgaggcggt atccaatgca gcggatgcgg ctatggctat caagaggttt gtctttgaag
     2161 agaaactttt tactttaccg caactggttg aaatattaaa aaatgactgg gccggggaag
     2221 agacgctacg taataaattc ctggccggtg ccccaaaatg gggtaacggg gacgcggaag
     2281 tagataatat cgccgcggag atgacagatt atttcttaga gcgggttaag tatcacgcaa
     2341 aatcatatcc ccagataata ttttcgccca gcatagccac cttttcatgg gttatatcca
     2401 tagggaaacg gatcggggcc tcggctgatg gcagaaatgc caaggaactg atcgcagcca
     2461 atctatcccc agcgcccggt cgtgataccg cagggcctac agcggctatt aattcttaca
     2521 tgaaaatcga tacctctacc atggccgcgg gggcacctat tgatcttagg gtgagtatga
     2581 atggtttaga aggtgaggag ggcactaagc ggatatccgc tctggtagag acatttctgg
     2641 aattgggcgg aaacatgatg accctaaccc taaccagcgc tgaagagcta aggcaggcca
     2701 tagaacatcc cgaaaactat agcgggctga gggtgaggat gggcggctgg tcggcatatt
     2761 ttaccctttt aagcaaggaa tcccaaaagc ttcatttgca tagggtagag cacaatctgt
     2821 agataagcgg tacggaagct aaacttattt gtagataata aattttacag ttggagttgt
     2881 ttatctatgg tccgactgtt tgagtcttct tgtcttcagc aagtcggtta ctgttgatag
     2941 caacctatga ttggggtgcc aaagctacat ttttctaaca agatacacca cataagggag
     3001 gacatttatt atgctcatag acatgccatt agaggaacta agagaatacc ggggtattaa
     3061 tccccgcccg cacgactttg acgagtattg gggaagtgcc ctcaaagaga tgagagccct
     3121 agatccgaag gtggaaatgg tgcccgcaaa gtttcaaacc cctcatgcag agtgtttcca
     3181 tctatatttt acaggggttg gcggtgccag gatacatgct aaatatctac gtcctaaagg
     3241 cgtcacaaag ccccatccgg ctgttttgat gtttcatgga tataccggca acagcggttc
     3301 ttggtctgac aagctggcct atgtaggaaa cggtttcgcc gtagcggctc ttgactgcag
     3361 gggacaagga ggcctgtccg aagatatcgg cggcgtgaag ggcaatacct tccgcggaca
     3421 tataataagg ggtcttgatg acagccctaa aaggcttttg ttcagggaaa tatttttgga
     3481 tacagcccag ctggccaaaa tcgtaatgga tatgccggaa gtcgatgaag accgtgtggg
     3541 cgctatgggc ggatcccaag gcgggggatt gaccctggcc tgcgcatccc tagagcccaa
     3601 aataaaaagg gttgcacctg tatacccatt tttatctgat tacaaaaggg tatgggagat
     3661 ggaccttgcc aagggcgcct atcaggagat cacggattat tttcggcttt ttgatcctac
     3721 ccatgccagg gaagaagagg tctttactac tttggggtac atagatgtgc agcatttgac
     3781 agagcgaata gaaggagaag taatgatggc cgtagggcta atggatacga tttgcccacc
     3841 ttccagccaa tttgcagctt ataacaagat catatcacca aaagagacca taatatatcc
     3901 ggactttgga cacgaggggt tgcccggatt tgatgatatg accttccagt ttatgatggg
     3961 gctgtaggac aataattatc cggaaagcgc cgaagattgt tgaacttgct ttgcgggtga
     4021 tgaccatagg cttgaccttt tgtgctatat atgttacgaa aaagaccttt caatgtaaaa
     4081 tcggcaaaac tatcctattt tacgttaaaa taatgtattt ttcgtaacat acgaattaag
     4141 gatagcgttt tacgactcct atgtaccaat gtagaatgtt gtcgtaaaac gtataggatc
     4201 attatttatg taattagcaa cccctgttga aaaaggggtt tttttctgga tataacttga
     4261 tttttatgac gaaatatgct ttaattatgt aaagtgagtt acaattctac aaaatatttt
     4321 aatggaattg taataaagcc taaacaattt aaaagggggt gaagaagtgt caaagagaat
     4381 gacaacaaga atttgtattc tcgcattggt aactttgatg ctcttggggc aagtttttac
     4441 atttaccagc cattcaaacg ctgcatcctc aactgttact caaaccttaa gacaagggag
     4501 cagaggcagt caagtaaaga tccttcaaca aaagctcaat tccctaggtt ttaatgcagg
     4561 cgatgtggac ggttcctttg ggccgaaaac tagggcggca gtgctggctt ttcaaaaagc
     4621 ctatgagtta gctagggatg gggtggtagg ccccgctaca agagcgaagc taaatccggg
     4681 gaagcccaag gattctaagc cacaagatcc taagccggca gatccaaaac cggagacatc
     4741 acctgcaaag gacataccgc aggttaccca aacccttaag aggggcagta agggaactca
     4801 ggtaaagtat ttgcaacaaa ggcttaacgc cttaggcttt aatgtaggtg aggtagacgg
     4861 ttcctttggg ccaaagactt tagcaggcgt tcatgccttt caaaaggcta acggacttgc
     4921 cgtagacgga tccgtaggtc cagctacaag agcgaaatta aacgcggcga taaagccgcc
     4981 taagccggaa gaaccaaaac cggagaagcc caagccggaa gaaccgaaac cagaaaagcc
     5041 aaaaccaaaa gagccaaaac cagagaaacc aaagcctgag acaccgcctg caaaggagat
     5101 accgcctgtt actcaaaccc tccgaagggg ggacaagggt acccaggtga agaacctgca
     5161 ggagaagctg aactatttag gttttgatgt tggtgaagtc gacggatcc
//
LOCUS       DASRNX010000061         6964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_436263,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000061 DASRNX010000000
VERSION     DASRNX010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436263"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VFD89_02430"
     CDS             <1..1024
                     /locus_tag="VFD89_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZJ57078.1"
                     /translation="SKWADALSIEDLKLPGYETMRARRFLDWIGPAIIRHGDGAPLRQ
                     ILDPDGTDPDGIIAHESKWEFRLYNKMDAIGIEILEDSITEPKDLLWEIGEEDEISIR
                     DEVINRLGWEYPHAQASRLPVKVSVTELKRHLDAEFSEEPTAMTVAMPALIKRPSFLE
                     EEAKGLTSAEWGSLLHFVLQHLDFKSATSKDAIREQIENMVEVQLITHEQARVVDILR
                     IERFLHSPLGIRMLKAEKIFREVPFTIELPSTRLYPELSGSICGDDGIILQGIMDCYF
                     EEPEGIILVDYKTDYVAPTGGLEMIRDRYKAQIDYYTYALEEITDKKVIGKYIYLFWN
                     GQTLEF"
     gene            1171..2025
                     /locus_tag="VFD89_02435"
     CDS             1171..2025
                     /locus_tag="VFD89_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZJ57079.1"
                     /translation="MSRFSIGVMVDSFRLPLEEGIAKSKEVGATGIQIYATSGEMAPE
                     NLSPARRKEILDLIKSHGLVVSALCGDLGGHGFAIAEDNPEKIRKSKMILDLARDLET
                     KVVTTHIGVVPEDPSHPRYKILQDACEELGEYGDELGAYFAIETGPETAIVLKDFLDS
                     LKSSGVRVNYDPANFVMVTGDDPVKGVYTLKDYIVHTHAKDGIMRQKSDPEKLYTFFA
                     EGGIEDMRLEDYFLETPLGEGHVDFPKWLKALEDVGYSGFLTIEREVGDSPEKDIREA
                     VGFLRGLI"
     gene            complement(2196..2936)
                     /locus_tag="VFD89_02440"
     CDS             complement(2196..2936)
                     /locus_tag="VFD89_02440"
                     /inference="COORDINATES: protein motif:HMM:NF039337.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HZJ57080.1"
                     /translation="MKQQIHTFPLWDRLKSRSECPFCEVLEETETSYISGMFRDMTTD
                     KEFIQHLRGNSFCNNHFTCLMKYRDKFGLALLLNQLVTYAMADLKSISIQNGPPLDKR
                     GPFQKLMEKFVSPAPPKTYHEPEDNKCALCTYLLERESDYTGTLIQLWGDDLHFRALY
                     HDSQGFCLKHFYSTIEMAPSILEGSDLDNFLDTSFYVQFQSMKQLNEDLKWFIKKFNY
                     EYSGEPWKNSRDCLARSIFKIRKKDPIG"
     gene            3836..4822
                     /locus_tag="VFD89_02445"
     CDS             3836..4822
                     /locus_tag="VFD89_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZJ57081.1"
                     /translation="MNPEARKAESPISDPRVYPGSGKANTSFWKKVRKNKTLLLMTSP
                     VILFFLIFSYLPMPGLYLAFIKYNYGEGILKSPFIGLENFRFLAQSGQLATITINTVL
                     YNIAFILLGNFLQILVAVLLNEVASTRFKKATQTIMFLPYFISAVIVGLLVYNILNYD
                     YGFLNVILKNLNLEPVSAYSTPAAWPFIIILTNLWQGVGYGSIVYFAQITSIDSEMVE
                     AASIDGVNAFQKIRYIILPWLKPTFVILLLFSLGGVLKGNFGLFYNLVGNNSLLLPTT
                     DIIETFVFRALMINFNFSQSSAVSLYQSIFGFALVLFCNWLVRRIEPDYSLF"
     gene            4839..5738
                     /locus_tag="VFD89_02450"
     CDS             4839..5738
                     /locus_tag="VFD89_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZJ57082.1"
                     /translation="MTRKAIRLDASSYFVKGFAYIFISLLTISCLIPFLLMIGTSFTN
                     ENAIREFGFNLWPRQFDLLAYRLVFENPDLVIGSYIVTILLTVCGTSIGLFLITMTGY
                     ALQRPDFEFRNQISFYIYFTTLFSGGLVPFYLLIVKILGLKNNYLAVFLPGLMSPWLI
                     ILMKNFTKSIPFAITESAKIDGANDFKIFASIILPMSKPALATVGLFLALSYWNEWYN
                     SMLFLEANMKYRPLQLFLYNVVNKAEYIRNSAASSNIPPIDVPQETMKMATAIVAIGP
                     IMLVYPFIQKYFIKGITIGAVKG"
     gene            6050..6247
                     /locus_tag="VFD89_02455"
     CDS             6050..6247
                     /locus_tag="VFD89_02455"
                     /inference="COORDINATES: protein motif:HMM:NF025693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4391 domain-containing protein"
                     /protein_id="HZJ57083.1"
                     /translation="MPIDDNITGARARELSAKIEDIEQEIISLRSKLKKETQFNRKME
                     LNIQIKKLEQSKNKLLGGDKK"
     gene            6244..>6964
                     /locus_tag="VFD89_02460"
     CDS             6244..>6964
                     /locus_tag="VFD89_02460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZJ57084.1"
                     /translation="MMETTLTGNTPDIAKENIKKLKTIFPEVFTEGKVDFEKLQQVLG
                     EYVEDSNERYNFTWNGKGRALRLSQTPSLGTLRPCKEESKDWDTTQNLYIEGDNLEVL
                     KLLQKSYYGKVKMIYIDPPYNTGKDFVYPDDFHDSLENYKKITGQVDSEGNRLSTNTE
                     ASGRYHTDWLNMMYPRLRLARNLLKDDGVIFISIDDNEVDNLKKICNEVFGEDNFIGS
                     IIWNSTKSVTNTALISVSHTYN"
BASE COUNT         2066 a         1344 c         1567 g         1987 t
ORIGIN      
        1 tagcaaatgg gcagatgcct tgtctattga ggaccttaaa cttcccggat acgaaaccat
       61 gagggcaagg cggtttttag actggatagg gcctgcaatc atcagacatg gtgatggagc
      121 acctttaagg caaattcttg acccagatgg taccgacccg gacgggatta tagcccatga
      181 atccaagtgg gaattcaggc tttataacaa gatggatgcc ataggcatag aaattttaga
      241 agacagcatc acggaaccta aggacctcct atgggagata ggcgaagagg atgagatatc
      301 cataagggac gaggttataa acagattagg gtgggagtat ccccatgccc aggccagccg
      361 gctgcccgtg aaggtatcgg tgacggagct aaagaggcac ttggatgcag agttttcgga
      421 agaacctacc gccatgaccg tggcgatgcc cgcccttata aagaggccat cctttttaga
      481 ggaagaggcc aaaggcctta catccgcaga atggggcagc ctgctccact tcgtcttgca
      541 gcacttagat tttaaaagtg cgacaagcaa ggatgctata agagagcaga tagaaaacat
      601 ggtagaggtt cagcttatca cccatgagca agcccgggta gtggatatcc tcaggataga
      661 gaggtttttg cattccccct taggcatcag aatgctaaag gcggagaaaa tctttaggga
      721 ggttcccttc accattgagc tgcccagtac caggctctac cccgagctat caggttcgat
      781 ctgcggggat gacgggatta tcctccaagg tattatggat tgctattttg aggaacccga
      841 agggattatc ctcgtggatt ataagacgga ttatgttgca ccaacggggg gtcttgaaat
      901 gatccgggat aggtacaagg cccagatcga ttactatacc tatgccctgg aagaaatcac
      961 ggacaaaaag gtcataggaa agtatatata cctcttttgg aatggacaga cgttggagtt
     1021 ctaaaggaag cgttgggggc gattcttatt gctttcaagg gaagtgatag gaatgattct
     1081 taccgctgtc aagctcttgt ttcttaacct gtagctataa ttttctaacc tgtgccttaa
     1141 actttcaaaa acaaataagg agttgatgga atgtcgaggt ttagtattgg tgttatggtg
     1201 gattctttca ggttaccctt ggaggagggt atagcaaagt ccaaagaggt gggcgctacc
     1261 gggattcaga tctatgccac atccggtgag atggcaccgg agaatctttc gcctgcaagg
     1321 aggaaggaaa tcttagatct cattaaatcc catggtttgg tagtctctgc cctatgcggg
     1381 gatttgggcg gacacgggtt tgccatagca gaggataacc ccgaaaaaat aagaaagtcc
     1441 aagatgatct tggatcttgc cagggaccta gagacaaagg ttgtaactac gcatatcggt
     1501 gttgtacccg aggatcccag ccatcccagg tataagatat tacaggatgc atgcgaggaa
     1561 ttaggtgagt acggggacga attgggggca tacttcgcta ttgaaacggg gccggaaaca
     1621 gctattgtcc ttaaagactt cctcgattca ttaaagagca gcggtgtaag ggttaactac
     1681 gatcctgcca acttcgtcat ggtaacaggg gatgatcccg tgaagggcgt atatacccta
     1741 aaggactaca tcgtacatac ccacgccaag gacggcatta tgagacagaa gagcgacccg
     1801 gaaaaactct atacattctt tgcagagggc ggtatcgagg atatgcggct ggaggattac
     1861 tttttggaga cacctctcgg tgaagggcat gttgatttcc ccaaatggtt aaaggcgctg
     1921 gaggacgtag ggtatagcgg ctttctgacc attgaaaggg aagtggggga tagcccggaa
     1981 aaggacatca gggaggccgt aggcttttta aggggattaa tatagttttt acagtagctt
     2041 attcgtaaag tcccatacac aaaggatatt tcaaactgaa ttagtctcaa cctttcccaa
     2101 tcggcggtga aatggatatt caatggctgc tgctatgcat aatattgatt agcgaaatat
     2161 gcttacccgc taattacaaa cttcagagaa agatcctagc ctatgggatc tttctttcta
     2221 atcttgaata tgctcctggc taagcaatct ctggaattct tccatggttc acctgaatat
     2281 tcgtaattaa acttcttgat aaaccatttt aaatcctcat ttaattgttt catactttga
     2341 aactgtacat agaagctggt atccaagaaa ttgtcaaggt cactgccctc taaaatactt
     2401 ggagccattt cgatggtgga atagaagtgc ttgagacaga atccctggct gtcatgatat
     2461 aaggccctaa aatgtaggtc atctccccat agctgtatca aggttcctgt ataatccgat
     2521 tccctctcta ataggtaggt acacagcgca catttgttat cttccggctc gtggtaggtt
     2581 ttaggcgggg caggggatac gaacttttcc attagctttt gaaaagggcc tctcttgtca
     2641 aggggaggac cattttgtat gcttatggac tttaaatcag ccatggcata ggtgaccaac
     2701 tggtttagca acagggccag accaaattta tccctatact tcatcaaaca agtaaaatgg
     2761 ttgttgcaaa aggagttgcc tcgcagatgc tgaatgaatt ccttatcggt tgtcatatcg
     2821 cgaaacatgc cggaaatata ggacgtttcg gtttcttcca gcacctcaca aaaggggcat
     2881 tcggaccttg attttaacct atcccacagg ggaaaggtat ggatctgctg cttcatcggg
     2941 atcctcctaa aatttctttg ttccatttca ttttttgcga ctttccacat attatacata
     3001 gacctaaagg gtacagcgcg caccatcatc acattgatct agacagtagg gcgtgcgaca
     3061 tcgttaattt ctgaatatgg attgatatat ttctgaatat ggtgttgaca ttaacggaat
     3121 tgggatatat aattaaaatg aaaccggtta cacagatatt atattacgca tctttggctt
     3181 cttattttgc ccatacaaca tagaatataa cgagacgtaa agatgtcccg cgaccatata
     3241 ttaagtggac acccattagt ccagacagat ggaggacaga aatcccccga tctcgttggg
     3301 acttatgtat cgaaatcact aatggagttt caaaagcaca ttatctcata tgttggacta
     3361 caatgcttat aattcagggt tttgaaattt caactggccg gttatttatc tatatgtttt
     3421 acgacaaaca ttctacatta gtatacgggt gtcgtaaaac attatcctca attcatatgt
     3481 tacgaaaaac acattgcttt aacgcaaaat cagataactt aactgatttt gtattgaaag
     3541 atctttttcg taacatatct agttaaccct acggtaattg acctagacat cccaatgata
     3601 caacctactt atatatttca cccatttgag gctactaagc tatcacataa tgcactttga
     3661 aacgattaag ctgttacctg ttacaccttg aaatgactaa gccatcacct gatacccata
     3721 aagcggagtt acatttataa ttgacggcgt ttgtgtgggg ctaaaaccgt tgctgttaac
     3781 ttattgggtc gggattatcc aatggatgat cgtattccga gaaaggggga tttatatgaa
     3841 tcctgaagct cgcaaagctg aaagtccaat cagtgatccc agggtatatc ctgggagtgg
     3901 gaaagccaat acatcatttt ggaagaaggt taggaagaat aagactctgc tattgatgac
     3961 atcacctgta atattatttt ttcttatatt cagttattta cctatgccgg ggctatattt
     4021 ggcttttata aaatataatt atggggaagg aattctcaaa agccccttta ttggcctgga
     4081 gaattttagg tttcttgccc aatccgggca gctggcaacg attacaatta atacagtcct
     4141 atacaatata gcctttatac tcctggggaa ttttctgcag atccttgtag cggtactgct
     4201 aaatgaagtt gccagcacca gattcaaaaa ggccacccaa accattatgt tcttacccta
     4261 ttttatatcg gccgtaattg tggggttact ggtctataat attttgaatt atgactatgg
     4321 ttttttgaat gtaatactaa agaacctgaa cctggagcct gtatccgcct actctactcc
     4381 tgcggcatgg ccctttatta taatactgac aaacctttgg cagggtgtgg gatatggttc
     4441 tatagtatac ttcgcacaga taacgagcat agattcggag atggttgaag cggcatctat
     4501 agatggtgtg aatgcatttc aaaagatacg ctatattatt ttgccttggc tcaaaccaac
     4561 ctttgttata ctgctcctgt tttccttggg cggagtatta aaggggaatt tcggtctctt
     4621 ctataacctt gtgggaaaca attcattgct attgcctaca acggacatca ttgagacctt
     4681 tgtgtttcgc gcgcttatga tcaattttaa tttttcacag agttccgccg taagtctgta
     4741 tcaatccata tttggatttg ccttggttct tttctgtaat tggctggtaa gaaggattga
     4801 acccgattat tcattattct aggcggggag gaagagatat gaccagaaaa gctataaggt
     4861 tggatgcgtc gtcttacttt gtaaagggct tcgcttacat atttatatcg ttattaacca
     4921 tttcatgcct gattccattt ttattgatga taggaacatc atttactaac gaaaatgcta
     4981 tcagggaatt tggatttaat ctatggccaa gacaatttga tctgcttgca tataggctgg
     5041 tctttgaaaa ccccgacctg gttatagggt cctatatagt aaccatatta ttgacggtat
     5101 gcggtactag cattggctta tttcttataa ctatgaccgg atatgcccta caaaggcctg
     5161 attttgaatt tagaaatcag atatcctttt atatctattt tacaaccttg ttttccggtg
     5221 gattggttcc cttctatctt ttgattgtga agatattggg gcttaagaac aattatctgg
     5281 cggtgtttct tcccggtctt atgtcccctt ggcttataat acttatgaaa aactttacta
     5341 agtccatccc ctttgctata actgaatccg ccaagataga tggggccaac gattttaaaa
     5401 tctttgcaag cataatactg ccgatgtcaa aaccagcgtt agccactgta gggttgtttc
     5461 tcgccctgtc ctattggaat gaatggtaca actctatgct atttctcgaa gccaatatga
     5521 agtataggcc ccttcaatta tttctatata atgtcgtaaa caaagctgag tatataagaa
     5581 attcagccgc cagttccaac attccaccaa tcgatgtgcc ccaggaaact atgaagatgg
     5641 ctacggctat agtagccatt ggacctatta tgctggttta tccgtttatc caaaaatatt
     5701 ttatcaaagg gataaccata ggtgcggtta agggctgata taaaccatag ggacacttta
     5761 tagtatttgc cttccggtgc ggggtattgc tcataaaaca tatggataag accccttcat
     5821 ataagggttt tattagactt cccaagaaca ctcgtgactt tagtcgtgag atgaattggg
     5881 gaatcccata aaagtagtgt tgtgtcctta acagggctat acagtagagg cgaactggac
     5941 acgcctgtac gcatcaaaat agcttaggct acagaggcgt aaatgagttc atttgaactc
     6001 tagaaccccg tggctttagt catgggaggt tcagaaccta tcagttatta tgcccataga
     6061 tgataatata acgggggcca gggcaaggga gctttctgca aagatagaag atattgaaca
     6121 agagattata agcttaagat ccaaactaaa aaaggaaacc cagtttaatc gaaaaatgga
     6181 gctaaatata caaataaaga agttggagca aagtaaaaat aaattacttg ggggagataa
     6241 gaaatgatgg agacaacact tacgggcaat actcctgata tagcgaaaga aaatatcaaa
     6301 aaactaaaga ccatattccc ggaggttttt accgagggaa aggtggactt tgaaaagctg
     6361 cagcaggtat taggcgaata tgtagaagat agtaacgaac gttacaactt cacatggaac
     6421 ggcaagggac gggccttgcg tctatcccag acaccgtctc tgggtaccct aagaccatgc
     6481 aaagaagaaa gtaaggattg ggatacaacc cagaatcttt acatagaggg ggataaccta
     6541 gaagtattaa agcttttgca aaagagctat tatggcaagg ttaagatgat atacatagac
     6601 ccaccctata acactggtaa ggattttgta tatcccgacg acttccatga tagccttgaa
     6661 aactataaaa aaatcacagg gcaagtggat agcgagggaa acaggcttag tacaaataca
     6721 gaagccagtg gccgttatca taccgattgg cttaatatga tgtatccaag attaaggctt
     6781 gctaggaatt tacttaaaga tgatggggta atctttatta gcatagatga taatgaggtt
     6841 gataatttaa agaagatttg taacgaggta tttggagagg ataattttat tggaagcatt
     6901 atctggaact cgacaaaatc tgtaacaaat accgccttaa tatctgtttc tcatacgtat
     6961 aatc
//
LOCUS       DASRNX010000062         6352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_596403,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000062 DASRNX010000000
VERSION     DASRNX010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596403"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1574)
                     /locus_tag="VFD89_02465"
     CDS             complement(207..1574)
                     /locus_tag="VFD89_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HZJ57085.1"
                     /translation="MSNYYDVLGIDQKASQIKIKKAYFGLVRKYPPDHYPDKFMEIRE
                     AYEALSNEETRREYDDTIILSPILKAEFEYSRKAFREGDLKTAIKGFEAVVKREPDSF
                     LAQDLLGQSYLANGNSVKAIRMFEKLTRKFPDNASFMGHLAESYLDRGWHNRARDAYE
                     KAVKLDEDNIAFWLGWSETYRIADEFEGQRHILYKALDKAKEGHWDSIDIYMHIILND
                     IFRGDMEDTEEHLAQLTDLVEDRVDIRDVIGVSLAELSDKLFSTGWPEMAIIIADHAL
                     QLLPEDNETEDLRKKINLLINTHNIMDEFTSDEDIHEDVKHLVAMKAIPDYLEDAGIP
                     GDVLCLMVQALIASELQIYLPSVHIIKERYPKIYEMEKDFFESLKNPRKRKSLRRRAD
                     KAYREYGDLLESFKGDDGFEDSFDNDYDFFEVQQPFVREHPKVGRNDPCPCGSGKKYK
                     RCCGR"
     gene            complement(1649..2239)
                     /locus_tag="VFD89_02470"
     CDS             complement(1649..2239)
                     /locus_tag="VFD89_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZJ57086.1"
                     /translation="MMKELTTHYFMRGMSLALEDRITLAVRKLETAIAMDSGNWEALN
                     VLGLCYYKMGEFSKSKVVWQESTKEYGQDNPAWDYLDTLKSQKFDEFCEKFNDALGLA
                     QQGNYKKAIKVLDGVRKQGFCSVSLLNLLGLCFYGNGKYGKAITLWKSVLTLDKDNPR
                     ANDYITKTMDKHPRKVYMAILMQSIKGIFYPHRDGI"
     gene            complement(2239..3966)
                     /locus_tag="VFD89_02475"
     CDS             complement(2239..3966)
                     /locus_tag="VFD89_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZJ57087.1"
                     /translation="MSKIIGIDLGTSTSEVAIYENGHTIVIPNLQGKKITPSVVGLSE
                     DGKILIGQDARDQMLVRPKETAIEVKRQMGKNIGISLGRKEYTPQQISSYIIGYLKEC
                     AEDYLGQSIDRAVITVPAYFTDEQRRATVEAGKLAGFKVERIINEPTAAALAYGIENM
                     ENNQFVLVYDLGGGTLDVTVLEMFEGVLEVKASSGNNELGGKDFDERLMDLLLEDFKS
                     QYQIDISTDLHSMARIKREAEVCKVELSRKESYLIQLPFIAEKDGQPLGLEKTITRSG
                     FEELIRDLIESSAQPIERALDDAGLEPDDLNLVFMVGGSTRIPLVQSFLEKNLKRTPV
                     SPLDPDLAVVMGAAIQGAILNDELSDEEDILITDVCPYTMGVEVMSFLGETPVDDIYD
                     PIIQRNLTIPVVKEKTYSTAGDGQTRVDIMVYQGEYKKASLNNFLGKFVLDGIPPGPA
                     FEEKINVRFSYDVNGILQVSGTIVSTNKEAGITIETTGVEMEEEAALDNWKEAPRARR
                     YRGIIRRGEKLLEELNDSEFGDIIILASLDTAIIELKKALLKEEDDGILEELEEDLND
                     IIYYIEDED"
     gene            complement(4239..4841)
                     /gene="grpE"
                     /locus_tag="VFD89_02480"
     CDS             complement(4239..4841)
                     /gene="grpE"
                     /locus_tag="VFD89_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZJ57088.1"
                     /translation="MINVKDEIRRYEPLDMDASFCDTDMELKTALNGFSKVLKRIDKE
                     QYRYSHLLDEILDVLDEIEEDKGAVRTLEGRQNEAKKELNSLLKAIIGIADSLEDIFI
                     YAMKYGDISFREQMSMQWKRIGNLLNQYGITRLEGIGSPFVPTLYVAKEVEEKQENPH
                     GHILEVLRSGYLYRGKLLRKAEVIVNKLVEQAANTNSSPQ"
     gene            complement(5112..5939)
                     /locus_tag="VFD89_02485"
     CDS             complement(5112..5939)
                     /locus_tag="VFD89_02485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZJ57089.1"
                     /translation="MRIDCHIHTTYGDVDREAFLSRLNQAGIDGGIVLSHGPSTLGGS
                     QISPQERIENVLEWTEGHKWLFPFYFIDPTGEKAMDEVELAASMGIMGFKIICNTHYP
                     SDPRAVPVYQQIANKGLPILFHSGILYDGKNASGNYNKPTEFEVLLSVDNLRFALAHV
                     SWPWTDECIAVYGKFNNSLKTGGDRAATAEMFVDITPGTPFIYRKSMLERLLLTGYDI
                     ENNVIWGVDSNVNNYGRDYALQISEFDDNVFNELQISEEIQNKIYFENVLRFIGALK"
BASE COUNT         1699 a         1525 c         1051 g         2077 t
ORIGIN      
        1 ctgttattac aaaccaaaac tctgtccgtt ctattatata tacccaatct tagcagctta
       61 aatctactaa aaatttaact tataaacagg gggacggggt tgatgacacc cactaacaca
      121 aggggacgta acaagaggag acggttcccc tatatttgcc ttatcttata gagtgtctcc
      181 aaccccgtcc ccttgacatc ctggcattat ctgccgcaac accttttata tttctttcca
      241 ctaccacaag ggcatggatc gtttcgacct acttttggat gttcccgtac aaaaggttgt
      301 tgtacttcga aaaagtcata atcgttatca aaactatcct caaagccgtc atcgcccttg
      361 aacgattcaa gtaagtcgcc atattctcta taagccttat ccgccctcct acgaagactc
      421 tttcttttgc ggggattttt cagcgactcg aagaaatcct tttccatttc gtatatttta
      481 ggatagcgct cttttattat atgaacagat gggagataga tttgcagttc actggctata
      541 agggcttgta ccatcagaca taaaacatca cccggtattc ctgcgtcttc taaataatca
      601 ggaatcgctt tcatagctac caaatgctta acatcctcat ggatatcctc atcactcgta
      661 aactcgtcca taatattgtg tgtattaatt aatagattaa ttttctttct gagatcctct
      721 gtttcattat cctctggaag caattggagc gcatgatccg ctatgataat agccatctcc
      781 ggccaccccg tactaaataa cttatcggac agctccgcca aactcacacc tataacatcc
      841 cgtatatcca ccctatcctc aacaagatcc gtgagctggg ccagatgctc ctccgtatct
      901 tccatatcgc ccctgaatat atcgtttagt ataatatgca tatagatatc tatgctatcc
      961 caatggccct ctttagcttt atcaagagcc ttgtataata tatgcctttg cccttcaaat
     1021 tcatcagcaa ttctatatgt ctctgaccat ccaagccaaa acgcaatatt atcctcgtct
     1081 agtttaacag ccttttcata cgcatcacgg gcccggttat gccatcccct gtccaaatac
     1141 gattctgcca aatgccccat aaacgacgca ttgtcaggga attttcgggt tagcttttca
     1201 aacattctga tagcctttac agagttaccg tttgccaaat aggactgtcc caatagatcc
     1261 tgcgctaaaa atgagtcagg ctctctcttt acgactgcct caaatccttt gatggcagtc
     1321 ttcaaatcac cttccctgaa cgctttgcgg ctatattcaa actcagcttt aaggataggg
     1381 gatagaataa ttgtatcgtc atattctctt cttgtttctt cgtttgataa ggcttcataa
     1441 gcctcccgga tctccataaa tttatcagga taatgatccg gaggatattt tctaaccaag
     1501 ccaaaatatg cttttttaat ctttatctgt gaggcttttt ggtctatgcc taaaacatcg
     1561 taataattac tcatatgttt tctcctttag gtaccagtaa ataaataatg gatcagccca
     1621 ggttattggt gacacccata ggattctttt agataccatc tctatgggga tagaatatgc
     1681 cctttatact ttgcattaat attgccatat aaactttacg gggatgctta tccatagtct
     1741 tggttatata gtcattagct cggggattat ccttatcaag agttaatacg ctcttccata
     1801 aggttatagc tttaccgtac tttccatttc cataaaaaca caatcccaaa agattcagta
     1861 aagaaacgga acaaaaaccc tgttttctta ccccatctaa aacctttatg gcctttttgt
     1921 aatttccctg ctgggctaat cccaaggcat cattaaattt ttcgcaaaat tcgtcaaatt
     1981 tctgagattt aagggtatcc aagtaatccc aggcagggtt gtcctgtccg tactctttgg
     2041 tgctttcttg ccaaacaacc ttggattttg aaaattcgcc catcttatag tagcaaagtc
     2101 ctaagacgtt taaagcctcc cagtttccgg aatccatagc tatggctgtt tccagcttcc
     2161 tcacagcaag tgttatccta tcttccaaag ccaaggacat ccccctcata aaatagtggg
     2221 tcgttaattc tttcatcatt agtcttcgtc ctcaatatag tatattatat cgtttaaatc
     2281 ctcctccaac tcttccaata tgccatcgtc ttcttccttg agcaatgcct ttttaagctc
     2341 tattatggcg gtatccaaac tggccaggat tataatatca ccaaactcag aatcgtttaa
     2401 ttcttccaac aatttttcgc cccgtcttat aattccccta tacctacgtg ccctgggtgc
     2461 ttccttccag ttatctaaag ccgcctcttc ttccatctct accccagttg tctcaatggt
     2521 tatgccggcc tctttgttag tgcttacaat tgtaccgctt acctgcaata ttccatttac
     2581 atcatatgaa aagcgtacat ttatcttttc ttcaaaggcc ggccccggtg ggataccatc
     2641 caatacgaac ttccctagaa agttgttgag agatgccttt ttgtattcac cctgatagac
     2701 catgatatct actctcgttt gaccatcccc agcagtacta taggttttct ccttaactac
     2761 cggaatggta agatttcttt ggattattgg gtcataaata tcatccaccg gggtttcacc
     2821 tagaaagctc ataacctcaa ctcccatggt gtaggggcac acatcggtaa tgagaatgtc
     2881 ctcctcgtcg gataactcat catttaagat tgctccttga atagccgcac ccattaccac
     2941 cgctaaatcc ggatctaggg gagaaaccgg agtcctttta agattttttt ccaggaagct
     3001 ttgcactagg ggaatacgtg tagaaccgcc taccataaag acaagattaa ggtcatcagg
     3061 ctctagcccg gcatcatcta aggccctttc tatgggctgg gcagaggact ctattaaatc
     3121 tcgaattagc tcttcaaaac cacttcgggt tatagttttt tcgagcccca ggggttgtcc
     3181 atccttctca gcaataaagg gcagttgaat aaggtaactt tccttgcgac tgagttcaac
     3241 cttacagacc tcagcttctc gctttattct agccatgctg tgtaaatctg ttgatatatc
     3301 tatttggtat tggcttttaa aatcctctaa taacagatcc ataagccttt catcgaaatc
     3361 tttgccgccc aattcattgt ttccgctact ggcctttact tccaaaacac cttcgaacat
     3421 ttcaagaacc gtaacatcca aagtgcctcc ccctaaatcg tataccaaca caaattgatt
     3481 attctccatg ttttcgatcc cgtaggccaa agccgccgct gtcggttcgt ttatgattcg
     3541 ttctacctta aagccggcca gtttaccggc ctccaccgtt gccctgcgct gttcatcggt
     3601 aaaatatgcc ggaacggtga tcactgcccg atctatggat tgacctaaat aatcctccgc
     3661 acattctttt aggtatccta ttatatagga cgaaatctgc tgaggcgtat actctttgcg
     3721 acctagggat attccaatat ttttacccat ctgccttttg acctctattg cggtttcttt
     3781 cggtcttacc agcatttgat ctcgagcatc ctgtcctatc aaaatcttac cgtcttccga
     3841 taaacccacc accgagggtg taattttttt cccttgtaga ttcggaataa ctatggtatg
     3901 cccattttcg tatatggcca cttctgaggt agatgttcca agatcaatgc ctattatttt
     3961 gctcataatc tgatttttca tctccattcc cgtcaataca tcacttaatt cagccgcaac
     4021 aattaaagaa attataaaaa atacattata cgatacttta gatatgttac gaattaggga
     4081 taatgtttta cgacacctgt gtaccaatgt agaatgtttg tcgtaaaaca tacagatcat
     4141 aatgttcata atccatcttt tttgaaagtt caactgaccc actgaccagt atttatttaa
     4201 tgatacctaa agcatctgat ataattgcac tagtggtttc attgaggcga ggaattggta
     4261 ttcgccgctt gctcaactaa tttattaaca ataacttcag cctttcttag taattttccc
     4321 ctataaaggt atccgcttct taagacctcc aatatatgac catggggatt ttcctgtttt
     4381 tcttccacct ctttagcaac atagagagtt gggacaaacg gagaacctat tccttctagt
     4441 cgagtgatac cgtattggtt tagaaggttt ccaatccttt tccactgcat agacatctgc
     4501 tccctgaagg atatatcgcc atatttcatc gcatatataa atatatcctc caaagaatcg
     4561 gctataccta ttatggcttt taagagggag tttagttctt tttttgcttc gttttgcctc
     4621 ccctccagag tccttacggc gcctttatcc tcttcgattt catccaacac atccaaaatt
     4681 tcatccaata gatgggaata tctatattgc tccttatcta ttcttttcaa cactttacta
     4741 aaaccattaa gggctgtttt taattccata tcggtgtcac aaaatgatgc atccatatct
     4801 aagggttcat atcttcttat ctcatctttt acgttgatca tatttacctc actcttcctg
     4861 taagcttaac acgagtctaa cttaatataa gtcatattct agcattacct attagaatag
     4921 gcaagaacac aaggggacac ggatagtgtc aacttacttt agggaaaagg gtggataatc
     4981 taaattatga tggaacattt attacgcctg ctcacaattt gatccggtct taacatatga
     5041 tatcttactt catcccccat attattttcc tttcccggtt tggtttttac acgcggttta
     5101 agacttgaat gctattttaa agcgcctata aagcgtagca cattctcaaa atatatttta
     5161 ttttgtattt cttcagagat ttgaagttca ttaaatacat tatcatcaaa ttcggagatt
     5221 tgtagtgcat agtccctacc ataattgttg acattggaat ctacacccca aatcacatta
     5281 ttttcgatgt catatccggt tagaagcaac ctctccaaca tggattttct gtagatgaac
     5341 ggggtgccgg gggtaatatc tacaaacatt tcggccgtag cagccctatc cccgccggtc
     5401 tttagggaat tgttaaattt accgtataca gctatacatt cgtccgtcca cggccatgat
     5461 acatgggcca gggcaaacct taagttatca acggatagaa gaacttcaaa ttcggtgggc
     5521 ttattgtaat tgcccgacgc atttttcccg tcatacagga tgccggagtg gaagaggatc
     5581 ggcaagcctt tattggctat ctgttggtac acgggcactg ctctgggatc gctggggtaa
     5641 tgggtattgc aaatgatctt aaagcccatt attcccatag aagcggccag ttccacctcg
     5701 tccatggctt tttcaccggt agggtcgatg aagtaaaatg gaaacagcca tttatgccct
     5761 tctgtccatt ccagcacatt ttctattctt tcctgcgggg atatttgtga ccccccaagg
     5821 gtagacggac catgggacaa gacaataccc ccatcaattc cggcctggtt caaccttgat
     5881 aagaaagcct cccgatctac atcaccatat gtcgtatgaa tatgacaatc aattctcatt
     5941 ttatcaccct tcccacaatt tttgattttt atgttgtttt ttatgttttt atgctcgccg
     6001 aattttagaa ggcatcaatc gcaccttttc cagcgggatt tttatgattt caatgttaac
     6061 cattcgctct tttacccctt tgttccccga tatccccgca ttagtccaat cagggatcga
     6121 tttgaatgcg tttatttcat gcaatcagca tcaatctctt tagtgccggt ataaattcag
     6181 cctcccccct gattgttcag cagcctcatt tgctttatcc ccaattaccg gtaaatcatc
     6241 atatttttat attattctct tatggataaa caactccaac tataaaattt atcatatata
     6301 gataaattta gtttccatat tgtttatcta tatatggtcc acccccttag ag
//
LOCUS       DASRNX010000063         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_226405,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000063 DASRNX010000000
VERSION     DASRNX010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226405"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFD89_02490"
     CDS             complement(<1..876)
                     /locus_tag="VFD89_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZJ57090.1"
                     /translation="MVRYFYRRFKEEQTSVDISAILDKQREYFSSGETRNPNFRLGKL
                     DRLKRAIEENEGDILRALQKDLNKAPFEAYETELGIVLDELRYIRKRLLPWIKPIRVR
                     TPLVHFPSSSFRIPEPYGIVLIMSPWNYPFQLTLAPLIGAIAAGNCSIIKPSNYSPAT
                     SQLISRIIRENFEDRYIAVLEGGREVNQTLLNHRFDKIFFTGSTAVGRVVMESAARFL
                     TPVTLELGGKSPCIVHRDCDIGLAARRIAWGKLLNAGQTCVAPDYLLVHGTIRHSLIK
                     AIKGYIIKFYGEDPIS"
     gene            complement(870..1886)
                     /locus_tag="VFD89_02495"
     CDS             complement(870..1886)
                     /locus_tag="VFD89_02495"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HZJ57091.1"
                     /translation="MQNQIIDPGNLLKADGSLEQKGYSTRSVLYYNRENIKAAPWRVK
                     EWDFYQINNDEYCIQMTIGHISYIGSVNVVAIDFKKGSRTCIETRPILPFNRLKMPRD
                     AEKGDLVYKSKNFQMAFSLSDGRRRLYAKIDDPKQPSVNLDIALEQPDKSSIVMATPF
                     DEDPHAFYYNHKINCMPASGIARIGGKEYRFDPEESFGLLDWGRGVWPFRHEWFWGSG
                     SGWIDEKRFGFNIGYGFGNTSAATENMIFYDGVCHKIDEVKFNIPEDSYSKPWTFTSN
                     DGRFEMDFVPVFDNYTEDKILFVNKHCHQVFGKFTGRATLDDGQVIEVKDIMAFAEHA
                     VNQW"
     gene            complement(1899..>3241)
                     /locus_tag="VFD89_02500"
     CDS             complement(1899..>3241)
                     /locus_tag="VFD89_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZJ57092.1"
                     /translation="VIVESAIYEVKIYRTIKRWDVFMENNRSISRKNWILIWIVGMAG
                     QICWNVENSWFNTFVYAKIAPDPSIIAWMVGVSATATTFATFLIGTWSDRIGRRKPFI
                     AIGYILWGLFTIAFGSAQFLPKNPILLVTVFIVATDAIMSFFGSMGNDAGFNIWTTDI
                     SNPQNRGKLGGALATMPVFATIFGAVVSGIIIDAVDFFPFFILMGAVVMLTGLLSIFT
                     LDDSPRLKPKRDEKGYWHQFISVFQIKTIMQNKELFWVLMIMVVYFIGFNVYFPYITI
                     YFVNYLGFDYTATGMIQGIGLVVASLFTIPAAKLIDRGKSPLVILIAVAVNFVGLFVV
                     TLSSKIPVILIGVLSVGIGYILTLQTLTAWNKNLYPETQRGQFEGIKQIFFVAIPMLV
                     GPWIATFIIKGYGVNGVPNESLFWFSAIINCFTLLPLVPAYKLHKARLQSINNK"
BASE COUNT          916 a          767 c          621 g          937 t
ORIGIN      
        1 gcttatagga tcctcaccat aaaatttaat tatatatcct tttatcgctt tgatgaggct
       61 atgccttata gtcccatgga ctaacaaata atccggagcc acacaggtct gccctgcatt
      121 taatagcttt ccccacgcaa tcctccgagc tgcgaggcca atatcacaat cccggtggac
      181 tatacaagga cttttgcctc ccagctccag agttaccggc gttaaaaacc ttgctgccga
      241 ttccataact acccttccta cagccgtgct accggtaaag aatatcttgt caaaccgatg
      301 attgagcaag gtttggttga cctctctgcc gccttccaga acggcaatat atctatcttc
      361 aaagttttct cttattatcc tggagataag ctgcgaagtt gctggagaat agtttgaagg
      421 cttaataata gaacaattac ccgcagcaat agcccctata aggggcgcca gggtcaattg
      481 aaaaggataa ttccatggcg acattatcag gactattcca taaggttcag gtattctgaa
      541 gctggatgag ggaaaatgaa ccagtggcgt tcttaccctt ataggtttta tccatggtaa
      601 aagacgcttt ctaatatacc ttaattcatc caagactatc cccaattcag tttcgtaggc
      661 ttcaaaaggg gctttattta aatccttttg cagggcccgc aatatatcac cttcattttc
      721 ttctattgct ctttttaggc ggtccagttt gccaagccta aaattgggat tccgagtctc
      781 gccactacta aaatattccc tctgtttatc caaaatcgca cttatatcca cggatgtttg
      841 ctcctcctta aaacgtctat aaaaatatct caccattggt taactgcatg ctctgcaaag
      901 gccattatat ccttaacctc tattacctgt ccgtcgtcca gcgtcgctct accggtaaat
      961 ttcccaaata cctgatgaca atgcttgttt acaaacagga tcttgtcttc ggtatagtta
     1021 tcaaaaacgg gtacaaagtc catttcaaat ctaccgtcgt tactcgtaaa tgtccagggt
     1081 ttactgtagc tatcttcggg tatattgaac ttcacttcat caatcttatg gcacactccg
     1141 tcatagaaga tcatgttttc ggtggccgct gaagtattgc caaacccgta gcctatgtta
     1201 aatccaaatc gtttttcatc gatccagcca ctcccgcttc cccagaacca ttcgtgccta
     1261 aaaggccaga ctccgcggcc ccaatcgaga aggccaaagg attcttcggg atcaaaccta
     1321 tattcctttc ctccaatacg ggcgattccc gatgcaggca tacaatttat tttatggtta
     1381 tagtaaaacg catggggatc ctcatcaaag ggagtcgcca taacaatgga ggatttatcg
     1441 ggctgttcca gggctatatc cagatttacc gacggttgtt ttggatcgtc aatcttagcg
     1501 tataatctac gccttccgtc agacagggaa aaggccattt gaaaattctt actcttatag
     1561 acaagatccc ctttttccgc atccctgggc atcttaagtc ggttaaaggg taggataggt
     1621 ctcgtctcta tacaggttcg gctgcccttt ttaaaatcaa ttgccactac gtttacagaa
     1681 cctatgtagg atatgtggcc aatggtcatc tgaatacaat attcatcatt gttaatttga
     1741 tagaagtccc attccttaac ccgccaaggt gccgccttta tgttctcgcg attgtagtac
     1801 aaaacgctcc gggttgaata gcctttttgc tctaaggatc catcggcttt tagcaaatta
     1861 ccgggatcta ttatctgatt ttgcatggat cttacctcct atttattatt tatggattgg
     1921 agacgggctt tatgtagctt atatgccggt actaaaggca acaaggtaaa gcagttgatg
     1981 atagcagaga accagaaaag cgattcgttg ggaacaccgt taaccccata cccctttatg
     2041 atgaatgtag ctatccaagg tcctacgagc atgggtattg cgacaaagaa tatctgcttt
     2101 atcccctcaa attgtccacg ctgggtctcg ggatagaggt ttttgttcca tgctgtaagg
     2161 gtttgcaatg taagaatata tccaatccct acgctcagca ctccaataag gatgacgggt
     2221 attttgctgg ataaagtaac gacaaataac cctacaaagt tgactgccac agcgatcaga
     2281 atcaccaagg gcgacttgcc cctatctatc agcttggctg cgggtattgt aaagagggat
     2341 gcaacaacaa gaccgatccc ttgaatcatc ccggtagccg tataatcaaa acctagatag
     2401 tttacaaaat agatggtgat gtagggaaaa tagacattaa agccgataaa atagacaacc
     2461 attatcataa ggacccagaa caattctttg ttttgcataa tagtttttat ctgaaatacc
     2521 gaaataaatt gatgccaata ccccttctca tcccgttttg gcttaagtct ggggctatcg
     2581 tccagggtaa agatcgatag aagaccagtt aacattacta ctgcccccat caatatgaag
     2641 aaggggaaaa aatcaacggc atctattata atgcccgata ccacggcacc aaatatggtt
     2701 gcgaataccg gcattgtggc taaggcaccg cccaatttcc ctctgttttg tggattggaa
     2761 atatcagtag tccaaatatt gaatcctgca tcatttccca tggatccaaa aaaactcata
     2821 attgcgtcgg tagccactat aaataccgtc actaagagga ttggattctt gggcaaaaat
     2881 tgcgcagacc caaaggcaat tgtaaatagc ccccatagga tatagcctat ggcgataaac
     2941 ggctttcgtc tgcctatcct atcgctccaa gtaccgatta agaatgtagc gaaggtcgta
     3001 gcagtagcgc taacccctac catccatgcg attattgagg gatccggtgc gatcttggca
     3061 tagacaaagg tattaaacca agagttttca acattccaac agatttgccc tgccattccc
     3121 acgatccata tcagtatcca attctttctg cttatgctcc tgttattttc cataaacaca
     3181 tcccaccttt taattgtcct atatatttta acttcatata tggccgattc tactataacc
     3241 g
//
LOCUS       DASRNX010000064         3014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_119052,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000064 DASRNX010000000
VERSION     DASRNX010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119052"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2552)
                     /locus_tag="VFD89_02505"
     CDS             complement(<1..2552)
                     /locus_tag="VFD89_02505"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HZJ57093.1"
                     /translation="MNNLIWHTKSLEEVEKVLQTSLDQGLSEQESARRLEKYGSNELE
                     HKGRKSIWGMLVEQFKDFMVIILIIAAAVSGILGETTDAIIILIIVGLNAILGVVQEN
                     KAEESLEALKNMAAPHAKVKRGGSPDVIDAANLVPGDVVVLEAGDFVPADIRLIETSN
                     VKIEEAALTGESVPVDKGNAPLPGDDIPLGDRVNMAYSGSIVTYGRGVGIVVETGMST
                     QMGRIAEMIQSEEGVRTPLQKRLESLGKILGIAALGICGLIFVVGVLYGKDIFDMFFT
                     SVSLAVAAIPEGLPAIVTIVLAIGVQRMAKRNAIIRKLPAIETLGSATVICSDKTGTL
                     TQNKMTVKQLYYNHMDQDIVDGSQNDNHHLELLILAGVLCNDTQIKETEEGLDTIGDP
                     TETALVDLGLNYGLDKRSLDQDIKRVYELPFDSERKLMTTVHGVDNGLRVFTKGGTDE
                     LLNKCNKILLNGNAEALTQQHIADIQRANEAMASKALRVLAMAYKDIDEIPNQEDGGQ
                     LESDLTLIGLMGMIDPPREEAKEAVKLCKRAGIKPIMITGDHKITAVAIAKELGILGE
                     RDEAITGAEVEAMRDEELKEKVKHISVYARVAPEHKVRIVKAWQEWGQIVAMTGDGVN
                     DAPALKRADIGAAMGIVGTDVAKEAADMVLTDDNFSTIVSAVEEGRIIFSNILKSIQF
                     LLSCNVGEILLLFIATMLNWREPLLPIHILWVNLVTDSLPALALGVDPAEADIMNRRP
                     RDPKRSIFDSGMIIRIVYQGTMVGLLALIAFVIGQRIDLITGRTMTFAVLALSQLIHT
                     FNVRSNTKSLFTIGFLTNIKLLWAIAFSALLQISVIVLPPLSNIFKVRALDG"
     gene            complement(2577..>3014)
                     /gene="dcd"
                     /locus_tag="VFD89_02510"
     CDS             complement(2577..>3014)
                     /gene="dcd"
                     /locus_tag="VFD89_02510"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZJ57094.1"
                     /translation="SYNLRLHNELLVYKNHVLDMKEENKAEKLIIPKEGLTLEPGRLY
                     LGRTVEHTRTERLVPMLEGRSSIGRLGLFIHITAGFGDVGFSGFWTLEIFCIQPIKVY
                     PGVEVCQIYYHSILGDYEEYKSGKYQNNQGIQPSLLFKDFDKR"
BASE COUNT          778 a          775 c          551 g          910 t
ORIGIN      
        1 cttccatcca aagcccttac tttaaatata tttgataaag ggggtaaaac gataacagag
       61 atctgtagca atgctgaaaa tgcaattgcc cataggagtt ttatgttggt taaaaaacca
      121 attgtaaata aggatttggt attggatctc acattaaagg tatgtattag ctgacttagc
      181 gccagaacag caaaggtcat ggtgcgtccg gttatgagat caatcctctg tccaatgaca
      241 aaggctatca aagccaataa tcccaccatg gttccttggt acacgattct aatgatcatc
      301 cccgaatcaa agatgcttct tttaggatcc ctgggcctac gattcattat atccgcttct
      361 gcaggatcta cccctaaggc taatgcgggc aggctatcgg tgaccaagtt gacccatagg
      421 atatgtatag gaagtaaggg ctctctccag ttaagcatgg tggcgataaa caaaagtaat
      481 atttccccaa cgttacagga taaaagaaat tgtatggact ttagaatgtt tgaaaatata
      541 attcggccct cttcaactgc ggatactatt gtactaaagt tgtcgtcggt aagcaccata
      601 tcagccgctt ctttggcaac atcggttccc acaatgccca tagccgcacc gatatccgct
      661 ctcttaagtg cgggagcatc gttgacccca tccccggtca tggctactat ttggccccat
      721 tcctgccatg ccttgactat tcttacctta tgttccgggg caacccgggc atagaccgat
      781 atatgcttga ccttctcttt aagctcttca tccctcattg cctcaacttc cgctccggta
      841 atggcctcgt ccctttcccc aaggattcct aattccttgg caatagcaac agccgttatc
      901 ttatgatccc cggtaatcat tataggctta atgcccgccc gcttacatag cttgactgct
      961 tctttggcct cttcccttgg ggggtcaatc attcccataa gacctattaa agtaagatca
     1021 ctctccaact gtcccccatc ctcttggttg ggtatttcgt ctatatcctt atatgccatc
     1081 gctaaaaccc taagggcttt agaagccatg gcttcatttg ctctttggat atcggctata
     1141 tgttgctgag ttaaagcctc tgcattccca tttagtaaaa tcttattgca tttattcagt
     1201 aactcatcgg taccgccttt ggtaaatacg cgcaatccat tgtctacacc atgcaccgtc
     1261 gtcataagtt ttcgttcgga atcgaagggt aattcatata ctctttttat gtcttgatcc
     1321 agcgatcgct tatctaatcc atagttaagt cctaaatcca ccaaagctgt ctcagtagga
     1381 tctcctatgg tatccaagcc ttcctctgtc tccttgattt gggtatcatt gcacaaaaca
     1441 ccggccaaga taagtagttc aaggtgatga ttatcatttt gactgccgtc cactatatcc
     1501 tgatccatat gattataata taactgcttt acagtcattt tattttgagt caaggtgccg
     1561 gtcttatcgg aacaaataac tgtggcactg cccaaggttt ctatagcggg cagctttcgt
     1621 ataatggcat ttcgtttggc catccgctgt acaccgatag ccaatactat ggttacaatg
     1681 gcgggtaatc cctcgggtat ggcggctact gccaaactca ccgaagtaaa gaacatatca
     1741 aatatatcct tgccgtagag gactcccact acaaaaatta gcccgcagat acccagcgct
     1801 gctatgccta aaatcttgcc taaggactct aaacgtttct gaagcggagt ccttacgccc
     1861 tcctcagatt gtatcatctc ggcgattctc cccatctggg tggacatgcc ggtttctaca
     1921 actattccta ctcccctgcc atatgtaacg atactgccgg aataagccat gttaactcta
     1981 tcgccaaggg gaatgtcgtc ccctggtaaa ggtgcattgc ccttatcaac gggaactgat
     2041 tcgcccgtca atgcagcctc ttctattttc acattggagg tttctatcaa cctaatatca
     2101 gccggtacaa aatcccccgc ttccagtact actacatccc caggaaccag gtttgcggca
     2161 tcaataacat cggggctacc gcctcttttt acctttgcgt gaggtgcagc catattcttg
     2221 agggcttcca aggactcttc tgccttgttt tcctgtacca ctcccaatat tgcgtttaaa
     2281 cctacaataa ttaggattat aattgcatcg gttgtctcac ctagtattcc ggataccgcc
     2341 gctgcaatta taagaattat taccataaaa tccttaaatt gttcaaccaa catgccccat
     2401 atggatttac gtcctttatg ctccagttca ttggaaccgt atttttcaag gcgcctagcc
     2461 gattcctgtt ctgatagtcc ctggtccaag gaagtttgta gtaccttctc cacctcttcc
     2521 aaggattttg tatgccaaat caaattattc atatcctcta ttctcttttc tcctatctat
     2581 ctcttatcaa aatccttaaa tagtagactg ggctgtatcc cctgattgtt ctgatatttg
     2641 ccgctcttat actcctcgta atctcccaga atagagtgat aataaatctg gcagacttca
     2701 accccaggat atacctttat aggctggata caaaagattt ccagagtcca aaacccgctg
     2761 aacccgacgt caccaaaccc ggccgttata tggataaaaa gacccagcct gcctatggaa
     2821 gacctaccct ccagcatggg aaccaacctt tctgtccttg tgtgttcaac ggtcctacca
     2881 aggtatagtc ttcccggttc cagtgttaaa ccttccttgg gaatgatcaa tttctcggcc
     2941 ttattttctt ctttcatatc taaaacatga ttcttataga ctaacaactc attatgtaac
     3001 ctcaaattgt agct
//
LOCUS       DASRNX010000065        33816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_278879,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000065 DASRNX010000000
VERSION     DASRNX010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 33816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33816
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278879"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /locus_tag="VFD89_02515"
     CDS             <1..1839
                     /locus_tag="VFD89_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZJ57095.1"
                     /translation="SGYVDNEYPKQQLIDYHVRRVIGGCAMNIVEIAAVHPTTKSPTI
                     LGIYDDKFIPGLTELAKAIKDAGGIACIQIWHGGRQTSGKPFGGQPVAPSVVTSAFIG
                     EEPRALTLEEVKEMISSYGDAAVRAKKAGFDAVEIHGAHGYLIDQFLNPYTNHRTDQY
                     GGSLENRARFGCEVIRDVRSKVGNDFPLILRMSAIEHVEGGIELEEAIEAAKLYVKSG
                     LDALDVSQGCYDALAYTVPPYYYPQKLNAYNASQIKKHTGVPMIVAGRITHPELAEEV
                     LQDGMADFIGLGRPLLADPDFIKKTTEGNTDDIVRCIACNQGCVGRMFNGLGNSCIFN
                     PATGHEREVIVKPASQKKKVLVIGGGPAGLEAARIAKIRGHEVILLEKDVALGGQFIE
                     AGHAPHKDIFSKAAIHMGYRAFKAGVDIRVYTAANRERIREINPDVVIVATGADPIIP
                     NIPGIHGPNVFEARKVIVSDQLIRADHVAIIGGGLIGLEAMEILSYQGKKVEIVEMTG
                     EIGKDLEMYIKPYVFGVIDEMSVVVHKNTKCIEINDESITVEKEGKQHRIPCGAVVIA
                     VGSKSNTSVADMAEELGYEYYIIGDAKEPSKLLPAIWGGNEIARSI"
     gene            complement(1909..2299)
                     /locus_tag="VFD89_02520"
                     /pseudo
     CDS             complement(1909..2299)
                     /locus_tag="VFD89_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590045.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2347..2432)
                     /locus_tag="VFD89_02525"
     tRNA            complement(2347..2432)
                     /locus_tag="VFD89_02525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2396..2398),aa:Leu,seq:gag)
     gene            complement(2900..4519)
                     /locus_tag="VFD89_02530"
     CDS             complement(2900..4519)
                     /locus_tag="VFD89_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZJ57096.1"
                     /translation="MSNILPIPQDEIQEDPKEEIGSSPQPTLPEAGGQLRIPVSLPES
                     INPLLTKSRDFINLSGLIYEGLFSYDKDLNLVPMLAESWEVTDGGKLWRFQLRRGVSW
                     HHGNELTAKDVKFTFDMLFNLAQELEGQDPGSVYARRLFDDRSVARMEFVINNPYAVD
                     LVLNEPAGRGLLEAMTFPILPADEGEFDFDPDEIESLNGTGLYKVDSGSLEIGEQVKL
                     IRNDDWWGISKPYIDSIIVKVYEDNVQSLEAFKKGQVDLVDTHVIYGKSYGTEGQAQS
                     YPYLTQHFNYIGINHNFPGILRDSDIRQAIAYGIDRKDIIPRVYSGNAQAVDAPIPPD
                     AWYYDSDLRMYDHQPQKARQLLAEAGWVDSYDDISDDDDEETLHPTFTINVHMDNILH
                     KEAVNLIAKQLEEVGIEVIIRLLPWEEYIIALEEGDFEAVFVEYYSDMAIDLRRLFYS
                     SDIPDGFNNYAGYSNEELDDLLDRIAQTDDPSEFKDAYREVQEILVDELPILSLYYRT
                     SSLIANERVHGIRAPRELMVFRDISEWYLNK"
     gene            complement(4604..5164)
                     /locus_tag="VFD89_02535"
     CDS             complement(4604..5164)
                     /locus_tag="VFD89_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZJ57097.1"
                     /translation="MKKILYWVVLLFLIGIVFIYWFLKSPIMQKRIYPLKYEEEILEF
                     SRQYDLDPHLVMGIIWVESKFVPEATSNKDAKGLMQIIPNTGKWIADQIGMDEYDEES
                     LYDPETNIRFGCWYFAYLLGLFDGDVELALASYNGGMGNVKKWLKDDRYSDDGRHLKS
                     IPFKETRDYLERVDDASKQYKKLYEI"
     gene            complement(5187..5819)
                     /gene="coaE"
                     /locus_tag="VFD89_02540"
     CDS             complement(5187..5819)
                     /gene="coaE"
                     /locus_tag="VFD89_02540"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZJ57098.1"
                     /translation="MEGKLEAIMRIIGLTGGIAAGKSRVSKILSKLGAKVIDADIIAR
                     EIVAKESPAWKKIRDEFGLGYFQADGELNRRKLGELVFSHRHALQRLNHIVHPAIRNN
                     IIDRLDELKIQNYTGIVVIDAALLLEVGWDDIADEVWVVDAPLEMRITRLMDRDGLSR
                     DGALKRMDSQMSQEDKNAKADRIILNDSDMDNLRSQVDRAWNQTLKEIYR"
     gene            complement(5876..8464)
                     /gene="polA"
                     /locus_tag="VFD89_02545"
     CDS             complement(5876..8464)
                     /gene="polA"
                     /locus_tag="VFD89_02545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZJ57099.1"
                     /translation="MDKKKMIIIDGNSLMYRAFYGIRALTTSKGVFTNAVFGFTNMLL
                     NLIEQYHPDYIGVAFDLKGPTFRHEIYEDYKANRQKTPEELLPQFPLLKTLLKAMNIA
                     VYEMEGFEADDILGTFSRLAQNQDFHAYLVTGDRDAMQLVSHDATVLLNRRGTTDMHI
                     FDPKEVEKVYGLRPDQMIDFKALMGDSSDNIPGVPGVGEKTALKLLSQYPTLDLVLDN
                     VDQISGKRLKENLTLYREQAFLSKELATIITDVPIDFNLEDCCYDMPKSAELKGILEE
                     LEFTSIINKLDLDDQGPSLEVLERVKETIEVKTLDGLQARVAKGLNKGRIAFIFDDDA
                     ITFSWDKRQVYYVKLFRDMLDGGLDYYEVLKALEPILKAREIGKIVHDGKKLLLEASK
                     LNIELNNLSFDTFIAAYLLDPTHTRYDVKYLLHEYLGIEVDIVDAADLLLLAHDLKKR
                     LEEVGMDDLYNEVEHPLIQVLADMELAGFKVNGEILRELDVEFTAILEGLTKSIYEIA
                     GQEFNINSPKQLGEILFEKLGLPIVKRTKTGYSTNIEVLEQLKDQHEIIGEIMEYRQV
                     MKLKSTYVDGLLNVIDPKDGRIHSSFNQTITATGRISSTEPNLQNIPVRMEMGRRIRR
                     VFVPSDEDHVLLDADYSQIELRVLAHISKDPTFIHGFENNQDIHRRTAAEVLGIPLEE
                     VTSEQRNDAKAVNFGIVYGISDFGLARNLGISRNKARLYIESYLKRHPGIKEYMDNVV
                     EKGKEQGYIKTLMGRRRNLPELSSRNYNIRSFGERIAMNTPIQGTAADIIKKAMNDVY
                     YELKSRGLRSKLVLQVHDELIIDAYRPELEEVKEILRDKMENAMELSVPLIVDIGVGS
                     SWYDVK"
     gene            8727..9557
                     /locus_tag="VFD89_02550"
     CDS             8727..9557
                     /locus_tag="VFD89_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZJ57100.1"
                     /translation="MRKSLVAFILTLTLAFSLLSTPAMAYYGRDWNQGQHVNNYINSG
                     SGYRSIPFFRQDTNGMLLRFNNWYVNSFNRVYQSGSGGNIQAPQPETRPEAPAPAPEA
                     KPEQPAPAPEYNPGPTQQPSQPTPAPQPEARPEQPVPAPQPAPTPQGIGGQQGQMLDM
                     VNQERIKAGLKPLAWDSDLANVAQVKAKDMSDNNYFDHNSPTYGSPFEMMKKFGITYR
                     AAGENLAGSGGVDRAHVGLMNSEGHRKNILNPNFTHLGIGVEKSTRYGYVYVQMFVGR
                     "
     gene            complement(9753..10529)
                     /locus_tag="VFD89_02555"
     CDS             complement(9753..10529)
                     /locus_tag="VFD89_02555"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZJ57101.1"
                     /translation="MDNKKLIYLLVGTLLVIGAVAITGIITAGRVAGSEVNLSTDRVD
                     DRARDNTLTVTGTGKVSIKPDVAYLEVGVRTVDKDAKIAQDENKDIMTKIVGKLASLN
                     IEDKDIQTSAYNIWPRYNYNGNREILEGYEVENMLRVTVRKIDSVGEVLDAVSREGAN
                     RSYGISFGILDTAAVYEEALEKAMDKAKAKAEVMAKKAGVILEKPLAVYEGNTPNEIY
                     PRGMTGINFVEDEMDSNGGVPIASGELEIEAHITIIYKVR"
     gene            complement(10522..11799)
                     /locus_tag="VFD89_02560"
     CDS             complement(10522..11799)
                     /locus_tag="VFD89_02560"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HZJ57102.1"
                     /translation="MGCKKIRYELSLYIDRRLVGRDLNKVEAHLNRCPDCREYYEDVM
                     KIKNSCKELGTVEVPPGFHSRLSEKLYKKGENEMLGRKHRVPIFIGLASVLVVIAIGI
                     TMVNTLGLGWMKSQSPAYDLAQSQSNRQPEAPQADGYVESEEDYMEMPSMEQGEMDQK
                     SDVGWGSDDSDISYDMGKEHISNAQRMIIKAAYLGIETLEFDVFTRNLEGKVKLMGGY
                     IESSNIQGISKETMGSFRPRQANYEIRIPNKKFEQFNNEVGELGNLITKEISGEDVTG
                     QYLDTQARVKSLTIQEERLLSILERAEILQDIIELESELSRVRYEIENYTGTLKGLEK
                     MVNFSRVNVDVYEVKEIKPTEPEPETLGERIAHAFKDSLKGMGRFLENMTIFLAGALP
                     FFVLLIPLGWIIWLLGAWLKSRRIRKGRSDRDG"
     gene            complement(11883..12827)
                     /locus_tag="VFD89_02565"
     CDS             complement(11883..12827)
                     /locus_tag="VFD89_02565"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZJ57103.1"
                     /translation="MDLITHGVVGLALGSLTGVPGIINNPLAIGSLIGSVIPDGDIIY
                     QLKGDYTYLKHHRGPSHSIAMGTIISLMISGILRGLFHDFSFWSIFAWTFGGYMLHIG
                     LDVFNSYGANILWPLNRKKIESGLLLIFDPALLISAGIISFSKGAHMGRIVGSIVFFA
                     LYLIFRWLLKRRVYKNLIRYFGNEADQEIVVMPSMLRLFKWDFILYKSAEVITGRVNT
                     LRGDIEVRDRLRRSSSGLESLVMDTNIGRFFKDFSPHYHVDWIKDGKNHRAIITDLRY
                     YVKGSYLHHATALFNSQFEPIYSAFQPYNKQREIKVPV"
     gene            13391..13477
                     /locus_tag="VFD89_02570"
     tRNA            13391..13477
                     /locus_tag="VFD89_02570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13425..13427,aa:Leu,seq:caa)
     gene            complement(13533..14255)
                     /locus_tag="VFD89_02575"
     CDS             complement(13533..14255)
                     /locus_tag="VFD89_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57104.1"
                     /translation="MSFYSQQNQGNERCPSLIGFDGLKRIKERVCIQVNKVYDSCLQQ
                     EVLENVRVNLTHVKGSGFDPPLTLVSCRSTSVTGKLVGTVIQRLEDRPNFARVRTKVE
                     IPIEVVFEDSRGKQGVGRAVICIPKDVIMFVPDESVIPFQLESVVNAICVSGMFIPGD
                     AFRFDLDVCITIILKIIAKVELLIPAFGFCEIPPCEEFASNVCDEFFRLPLFPPQLED
                     MPIDPKPCPRPRLLGQEDEVEE"
     gene            14480..16429
                     /locus_tag="VFD89_02580"
     CDS             14480..16429
                     /locus_tag="VFD89_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57105.1"
                     /translation="MDGIINFESTSFLQDREGTIWFFYLSDNQRIGYAVNIQGDWSAH
                     EHIDSQPIKGYSATLDSKGIIRLIAYTATHQLIYYEFVGGKWNSQVIERIYSRYQDIP
                     YYSIRSSVLGIHILYYINHSLSRSGETLIHYYLQDGRWNGGRLWKFVSDQMTMVRDLY
                     IDSHNNLQLLFTQRWRQKHHLYHCVYDHATLSWMDPVNIHSAVSSYDYRLFVGSSDDP
                     HILWHTEMEGNRHIHHLFKSGPPQDSPSWQEALIHETPHSIDTPVLMEDDNGLYCIWK
                     EDGLIYKRLSHDLGRTWSTPQVATDSMNYDASLLDFAYLDNGIPRSLSLWGQGYPAMR
                     LAVADDKIVIDRVKMQDSPKPPQPSPDLDQGKRDSLAIADIKRTIASIRRKNSGLSED
                     IGNLSVQVDRLNALVYTLQEQIQINDRSLFNIHAQIKQLDFQIKQLQLRSRQVSPRVY
                     SADDSRYRPLPITLDQDGKILVESQHLPADVSADVEEDEGMEPLEENESKLIHKEDSN
                     PLNNNQTYAESKSSPKVKPPQTGDSTGGPKKDPPMGGGNTPKETKPKVHGDGPVVSES
                     TGDSISMEDSISTEDRKSMGDRKSTKESTSPRAIVTVEAVVSPEDDDPPETDSHPEDK
                     SNRDIERISLGNTTILINPENPEDF"
     gene            complement(16398..17006)
                     /gene="yihA"
                     /locus_tag="VFD89_02585"
     CDS             complement(16398..17006)
                     /gene="yihA"
                     /locus_tag="VFD89_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HZJ57106.1"
                     /translation="MEIVKAEFITSAVESHQYPTDHLPYIALAGKSNVGKSSVINTIT
                     RRSKLAKTSGQPGKTRLVNFYRINETFYLVDLPGYGFARVSRKEKERWGYMIETFLSA
                     SPNLLGIIQLIDIRHNPTDDDITMLNWIGHYDMPLIIVATKADKLGKTRIKPQIDKLR
                     NKLGLSADGPMIPFSSSTGQGREVLLKSLDSLFKNPLDFQGL"
     gene            complement(16987..19344)
                     /gene="lon"
                     /locus_tag="VFD89_02590"
     CDS             complement(16987..19344)
                     /gene="lon"
                     /locus_tag="VFD89_02590"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZJ57107.1"
                     /translation="MSKKDIKTRQMPLLPLRGLTVFPYMVLHFDVGRQKSISALEDAM
                     VNNQDIFLVTQKEPEVEEPDKDSLFTVGTVSKIKQLLKLPGDTIRVLVEGINRGRIVE
                     FLDNDKFFEVLIEEQPDQEDSDPLEEEALMRTVMDAFEEFIKLSNKISPDTLVSVNTV
                     DDIGQFADIVAANVLVKNSNKQEILEAFRPMERLQILHKFLENEIQILRIEKKINLRV
                     KKQIDKVQREYYLREQMKAIQKELGESDSISSDVEDYNKRIEKASLPEEVHEKAVSEL
                     ERFSRTPLTSPESSIIRTYLDWVLDLPWNHETKDNHNLKSAARILDQDHYGLDKVKER
                     VMEYLAVLQLTQNMKGPILCFVGPPGVGKTSIAKSIARALGRNFVRMSLGGVRDEAEI
                     RGHRRTYVGSIPGTIISSIKQAGSKNPVFILDEIDKMSGDFRGDPASAMLEVLDPEQN
                     FTFRDHYLELAYDLSKVMFLTTANTLDTIPRPLLDRMEVIRIAGYTEEEKTNIALKYL
                     IPKQLEEHGLKRGNIIFPEQTIRSIINHYTREAGVRDLERQIAAICRKGVRFLLEAGR
                     KRIRITPKNLHEYLGIPRFSFNKANQKDEVGLVTGLAWTAVGGDTLYIEVTPMVGTGK
                     LVLTGQLGDVMKESARAGLSYIRSKAKDFDIDPDFYNNTDIHIHIPEGAIPKDGPSAG
                     ITIATAIISALTETPVRRDIAMTGEITLRGRVLPIGGLKEKILAAHRAGIAKVIIPDE
                     NTKDLEEIPDNIKEEIEIVAVQNMDEILEHALSGREGDRDGDSKG"
     gene            complement(19762..21447)
                     /gene="lonB"
                     /locus_tag="VFD89_02595"
     CDS             complement(19762..21447)
                     /gene="lonB"
                     /locus_tag="VFD89_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HZJ57108.1"
                     /translation="MGNILAVINLFFAVVIGLYFLNLLKGQQGNKSAVERESRKELER
                     LQRLREISLSEPLSEKTRPNVFNDIVGQEEGIKALKAALCGPNPQHILIYGPPGVGKT
                     CAARIVLDEAKKNPMSPFRKDAKFIEMDATSVRFDERSIADPLIGSVHDPIYQGAGPL
                     GVAGIPQPKPGAVTKAHGGVLFLDEIGELHPIQMNKLLKVLEDRKVFLESAYYNSEDS
                     NIPRHIHEIFKRGLPADFRLVGATTRNPEEISPAIRSRCLEIYFRGLTQEENMIIARN
                     AADKAGFHMGEDSVELIGKYASNGRDTVNIIQLAVGLALTENRNHILVEDVEWVINSG
                     HYSPRPEKKVLDSPQVGYVNGLAVYGANLGTLIEVEASALPSIKGGGIVTITGIVDEE
                     EIGSGVKKSRRKSTAKSSVDNVMTVIRKFLDVDPRDYDIHLNFPGGIPIDGPSAGISI
                     VIAIYSAITGLPIDNNSAMTGEISIRGEVKPVGGINAKLHAAVGAGVKKVIIPKDNWN
                     EQHSAYGIEIIPVRRIEEVIEQVVCKGREEQNLEPMIQNAGLLSASGRRHYTT"
     gene            complement(21804..23090)
                     /gene="clpX"
                     /locus_tag="VFD89_02600"
     CDS             complement(21804..23090)
                     /gene="clpX"
                     /locus_tag="VFD89_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZJ57109.1"
                     /translation="MARYDDKKQLKCSFCSKTQDQVRRLVAGPGVYICDECIELCQEI
                     IEEEFDDASDLDIRDIPKPIEIREILDQYVIDQEAAKKTLSVAVYNHYKRINSDVAKD
                     DVELYKSNIVMLGPTGSGKTLLAQTLARILNVPFAVADATSLTEAGYVGEDVENILLK
                     LIQAADYDVERAEKGIIYIDEIDKVARKSENPSITRDVSGEGVQQALLKILEGTVASV
                     PPQGGRKHPHQEFIQIDTTNILFICGGAFDGIDKIIQNRIGKKSMGFGAEIRSNVNYD
                     VGETLEKILPEDLLRYGLIPEFVGRIPVVVTLNSLDEGALVQILKEPKNALVKQYQKL
                     LEMDGVALEFEDEALRLIAKRAVERKTGARGLRAILERIMLNVMFDIPSRDDVEKCII
                     TKETIINNSDPILILSEGQRSRKGMKKTKDKRESVS"
     gene            complement(23337..23921)
                     /gene="clpP"
                     /locus_tag="VFD89_02605"
     CDS             complement(23337..23921)
                     /gene="clpP"
                     /locus_tag="VFD89_02605"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZJ57110.1"
                     /translation="MNLVPIVVEQTSRGERSYDIYSRLLEDRIIFLGGPISDLTANLV
                     VAQLLFLEAKDPDKDVLIYINSPGGSVTAGMAIFDTMQYLKCDVSTICVGMAASMGAF
                     LLAAGTKGKRLALPNSEIMIHQPLGGAQGQASDIAIQAEQILKLKKKINEILAERTGK
                     PLDRIEKDTDRDFFMSAEDAKSYGIIDDVIVSRK"
     gene            complement(23970..25304)
                     /gene="tig"
                     /locus_tag="VFD89_02610"
     CDS             complement(23970..25304)
                     /gene="tig"
                     /locus_tag="VFD89_02610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZJ57111.1"
                     /translation="MKANVERLENSKVKLEIEVDAKQFDEAMQKSYLKNRGRISIPGF
                     RKGKAPRQIIERYYGEGIFYEDALAETLPTIYDEAVEEHELFPVDQPDFDVVQIGDGQ
                     DLIFTAEVAVKPEVVLGQYKGIEINKVEYNVTDQDIDDQLERSREQNARWITVEDRSV
                     EEGDRITLDYKGYVDGEAFEGGTAENQTLEIGSGQFIPGFEEQLVGMDAGSEGEVKVT
                     FPEEYHSEDLKGKDAIFEVKVHEIKEKELPELDDEFIKDISEFDTVEEYRADLKERMD
                     EDAARRGKSEMENLLLRKIVENMEADVPEVMIEREIDTSIREMDTRMRYQGMDLNSYL
                     GMIGSSMDNFRSDMSDEANHRVRLQLALEQINKEEAIEATDEDVEKEYNTIAEEYKLD
                     LDEVKKRYEGQEDGLRHSLSIQKTIDFLMEQAVIVEAPEEDAKPEEQQTQEG"
     gene            25673..26446
                     /gene="fabI"
                     /locus_tag="VFD89_02615"
     CDS             25673..26446
                     /gene="fabI"
                     /locus_tag="VFD89_02615"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HZJ57112.1"
                     /translation="MNTLLKDKTILIMGVANKWSIAWGIAEACIDAGANIIFTYQGER
                     NLSSLQKLTANLPNASLYKCDVTVDQDITDLFHDIGNDFGTVHGIVHSIAFAKKEELE
                     GAYYNTSREGYHLAQDISAYSLVAVSRHARPLMQDGGSILTLTYLGGERAVQNYNVMG
                     VAKSALESSVRYLAADLGPENIRVNSISAGPIKTLAAQGVKGFGSILKVYEEKVPLRK
                     TVTQREVGDTAVFLLSQMSRGITGENIHVDAGYHAMGLL"
     gene            complement(26542..26826)
                     /locus_tag="VFD89_02620"
     CDS             complement(26542..26826)
                     /locus_tag="VFD89_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HZJ57113.1"
                     /translation="MDLKLFFLSFWLLFVAELGDKTQLAVFTLVTQHKQPWPIFLGAS
                     LGLTVVTLLGAFMGGFVCKYIPAAILKMAAGIMFLVMGFFILKECLGELF"
     gene            complement(26858..28006)
                     /locus_tag="VFD89_02625"
     CDS             complement(26858..28006)
                     /locus_tag="VFD89_02625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZJ57114.1"
                     /translation="MESDSRPRVAELIRRAEGGMKEHYMALTEGLIGNGMDVIALCSL
                     SRGDRKRLSKWGATVVPFVIRGRISPIRDILAILRLARLLRRYRVDILHCHGFRAGLI
                     GRIAALFVRCPCIYTIHNFFPKSLGKLGTMGIAWAEKRLSGITKNIITVSGALKKYTA
                     KGLAIDPDKIKIIYNGIDPPNYIGDDHDIRQMWGIEPEEKLVGTVARLIPAKGLDVLI
                     DAVPPVLEEFPDTKFMIVGDGPDRSNLMDKAAHLNCSKNIIFAGHSEYIWYYYEAFDI
                     FVLPSLSEGLGISILEAMAMGKPVIATNVGGIREIIDHNRNGYLIPPGDSGELADALL
                     FYLENEQRTYEYAKVGRAEILTKFRLEDMIRETTQTIYEVIGRQYSRC"
     gene            complement(27966..30284)
                     /locus_tag="VFD89_02630"
     CDS             complement(27966..30284)
                     /locus_tag="VFD89_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57115.1"
                     /translation="MKRIHTYPILKITFCFLLMLPILAIPNNSILCGHRPEALRQRDH
                     KIEDRGRVILILLDRVSWQDIHDADTPNLDRLMGIGAAGMMTTNTGGSLSQNNAYLTL
                     GSGARVTGLAGSQVAASYGYPYRGEKIENLMHQITGYRMDEGAIANPSIAKLHRNNEN
                     RPYRVKVGALGMALREAGVGVAVLGNCDNYRVAEGEVGGKHFLVSMMMDERGIVPHGD
                     ISQSLLTDDPHWPMGIRTDYDGMLKAFNEFSGESHVIAIQLGDTSRAEDFRHQSMDSR
                     VEYHKRRALEAGDKFIGGILEYADLQNDYMMVLTPVGPAKEIGNNNRLTPIILAGRGI
                     QGGWLSSGSTHRRGIVTNLDVGASILNFFDLMPLTGQGGAPIYSLPGNTGKDELVRFN
                     SGLVDIYNQRPFLIKGYVYTLIPILGLAAAVLLFKRKYLGHIKPMLIYIMVMPFVYLV
                     LPMLQKPQLYRTALIAISLSVIITGILCRWIKSTMDKVLIISGLTLGALMLDQWLGMQ
                     LIKASPLGYDVIAGARFYGMGNEYMGVMVGAACTTIAAVYQRFINRKRVAVVFMLITG
                     SLALFTLASPGLGANVGGTMAMFAALSSLALIIRNRKIRIHSVISMAAIMIVLLAGIF
                     FYDSLRVVDSQSHMGQTANAVKENGLGELLDIFHRKISMNIRLFRTTIWTRVLFTSLG
                     VMVLLLYRPVGIFRDMYRVHRIFLRGLMGAIIGAVAALIFNDSGIVSAATAMIFVAPP
                     FVLLIGDEVQRKVMNGVWEDGIRFKAQGRRTD"
     gene            complement(30576..31511)
                     /locus_tag="VFD89_02635"
     CDS             complement(30576..31511)
                     /locus_tag="VFD89_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZJ57116.1"
                     /translation="MKEKEGSIFTNSYVLMALALISCFLWGSAFPSVKSGYKLFSIGP
                     DATYEKIVFAGYRFFLSSMMIFGFCLVTGRSLKLKRAHVRKVVSLGVMQTSIQYVFFY
                     IGLSNTSGTLGSILSASSTFFTVLLAHFFFSQDKLTHKRIAGAVLGFIGVITANMTGT
                     GLGTDFTLTGEGFIIISSLVGAFAGFYTKKISKDISPFAISGYQLFFGSLLLISFGWL
                     GGGRSITFTPMGILLLLYLGFISAAAFTLWSILLRHNGVGRVSIYKFTIPLFGTLLSY
                     IILKERFLGANIIAAAILVSLGILLINKGESITHD"
     gene            complement(31619..32593)
                     /locus_tag="VFD89_02640"
     CDS             complement(31619..32593)
                     /locus_tag="VFD89_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57117.1"
                     /translation="MMKHKGFIGMLFVAMCIFALAGCQLARADAGEEPTGDRLIGVFV
                     TDEYLDLFDFEGYLNDNFGGISDGGIITEDQSRRYEGRLYAVLKELELVDEETGETST
                     TKEYVFEGVNGASYFAATITEEDGNTSYTTTGSDETISDGHTGIFIGDDEEKTTLEGI
                     IYIAATPGMISRYINPVYQGADGRVYAVSGSGMTMDGAENEGGAFSQTIEETTTITEN
                     GESKKHSTSVKISLETMFSPVKIAILEMDENGSVLSHREYSPGQVPDRLAPNKQTEYI
                     IVETHKQDSSGNALITRELIGREETNFTTFFRRGDDICVKRWTDLLWE"
     gene            complement(32580..32810)
                     /locus_tag="VFD89_02645"
     CDS             complement(32580..32810)
                     /locus_tag="VFD89_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZJ57118.1"
                     /translation="MAIKVHLEEVLKDRGMTSKELSALVGITEANLSILRSGKAKGVR
                     FNTINKICWHLKCDVGDILKFDGNLEDEDDEA"
     gene            complement(32820..33788)
                     /locus_tag="VFD89_02650"
     CDS             complement(32820..33788)
                     /locus_tag="VFD89_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57119.1"
                     /translation="MNRRIYSLILATEAAVCLIFAAMGSLTHELFPAVVSFPFAQLGS
                     GLRILSLSGQVGNVAAILFYIAFCIAPCAALLIHKRKRDFYKEDILLLVFSALLFVIM
                     YLLINPGLINQDSGSNGGILFAKGVFGVTVYSVIGGYAILRALRLFFFSDTKKLHKYM
                     IGLLVLVNALFVFIIFGPGANNLINSFKALRVGNLGNERLLGMDYVFLVLQFLVDNIP
                     LMLNILIVNRGIALIGELAENRYSVASVSEAENLSIICRVSLVITVLGTIAFNILQLI
                     FSKFLHNINSQVQIPLFSIVFVLAVLLFSRITAENKVLKEDSDSII"
BASE COUNT         8942 a         8419 c         6726 g         9729 t
ORIGIN      
        1 tccggttatg ttgataatga ataccctaaa cagcaattga tagattatca tgttcggaga
       61 gtaataggcg gatgcgctat gaatattgta gagattgcag ccgttcatcc aacaacgaaa
      121 tcccccacga ttttagggat atatgatgat aagtttatac cgggccttac cgaattggca
      181 aaagcaatta aggatgccgg aggtattgcc tgcattcaga tttggcacgg gggcagacag
      241 acatcaggta agccctttgg cgggcaaccc gtagctccgt ccgtagttac cagtgcattt
      301 attggggaag agcccagagc cttgaccctt gaggaagtta aggagatgat ctcgagctac
      361 ggcgatgctg cggtaagagc aaagaaagcc ggcttcgacg ctgttgaaat tcatggtgca
      421 catggctatc taatagatca attcctcaac ccctacacca accatagaac cgatcaatac
      481 ggaggcagcc tcgaaaacag ggctcgtttc ggttgtgagg taattaggga tgtccgttca
      541 aaggtaggta acgatttccc gcttattctg cgaatgagcg cgatcgaaca tgtagaaggc
      601 ggtatagagc tggaggaggc tatcgaggcg gcaaagctat atgtaaaatc cgggcttgat
      661 gcgttggatg tttcgcaggg ttgctacgat gcattggcgt ataccgttcc gccttactat
      721 tacccacaaa agctaaatgc ctataacgct tctcaaataa aaaagcatac aggtgttcct
      781 atgatcgtgg ccggaagaat tacccatccg gagcttgccg aggaagtgct tcaagacggt
      841 atggcggact ttatcggcct gggaagaccg ctgctggcag acccggattt tattaaaaag
      901 accacggagg gaaacaccga tgatattgta cgctgtattg cctgtaacca gggttgtgtc
      961 gggagaatgt ttaatggcct tgggaacagt tgcatattca accccgccac cggccacgag
     1021 agggaagtaa tagtaaagcc cgcctcgcaa aagaaaaagg tactggttat agggggaggt
     1081 cctgcgggat tagaagcagc taggatagca aagatccgag gtcatgaagt tattctttta
     1141 gagaaagatg ttgccttggg cggacaattt atcgaagccg ggcatgcacc ccataaggac
     1201 attttctcca aagcggcaat ccatatgggg taccgagcat ttaaagcagg tgtcgatata
     1261 agggtctaca ctgccgcaaa tagggaaaga atcagggaaa ttaaccctga tgtagtcatt
     1321 gtggctaccg gggccgatcc cattataccg aatatcccgg gaatccatgg ccccaacgtg
     1381 tttgaggcaa ggaaggttat agtttcagac caattgatca gggcagatca tgtagctatt
     1441 ataggcggag ggcttatcgg gctcgaagct atggaaatcc tcagctacca aggcaagaag
     1501 gtggaaatag tggagatgac gggcgagatt ggcaaagacc ttgagatgta tataaagcct
     1561 tatgtcttcg gtgttataga tgaaatgtcc gtggtggtcc ataagaatac gaaatgtatt
     1621 gaaattaatg atgaatccat cactgtagaa aaggaaggaa aacaacacag gattccttgt
     1681 ggtgcggttg tcattgccgt gggctctaag agtaacacat ccgttgcgga tatggcagag
     1741 gaactaggct acgagtatta tataattggc gatgccaagg aaccatcaaa actcttgcct
     1801 gccatatggg gaggaaacga aatcgccaga agcatctagg ctaatgcttt tttgttaacc
     1861 tgttgtgcta gctcaatatg tcggtaacga actagcacaa caggttaact aataaatcgc
     1921 taccggcccg tggggtccgc ggataatttc tatttccgta tcaaaaatat cggttaaaat
     1981 ttcttgatcc attacttctt ctaccggtcc aaataccgca atttttccgt ccttcatagc
     2041 acagatccta tcggaatatt gcgccgcaaa gtttatatca tgcatcacgg ttaaaatcgt
     2101 tcttccaaac tcattggcaa cgtgtctcaa gtgcttcatc atctgtacag atcgggcaac
     2161 atcaaggttg ttgaatgcct catccagaag caggtattcg gtctcttggc ttaaagccat
     2221 agccacatat gccctttgcc tttgtccccc cgaggagctc atctaaatat ctgttttcca
     2281 gatcggtgag gctcaagtag aaagagaaaa gccatgtatg gattttccca tacatggctt
     2341 ttaaaatggt gcgggtgatg ggacttgaac ccatacgtcg gttagacata cggccctcaa
     2401 ccgtacctgt ctgccaattc cagcacaccc gcaaatattg attttgatat caccaaatag
     2461 ataattgatg agcttgggtt tcaaaaaatg aaattatcaa tattatagac taaaacgcca
     2521 tctattataa catcggaagc aattgcattg gcaattgcaa cacacaggtt tcaacgaggc
     2581 gggtgattta tacccaccgc ctgttttact aatcggagct tactcgccgc ttataaaagc
     2641 gggagacatc ttaccgactc gttatatttt tgaaactccc tcgctgcatc tattttagag
     2701 taacaagtta tattataata tcccatgggg ctcctgtcaa gggcgaaaat ttttatcccc
     2761 cgggcaccag cttgcagtat tgtgcggact ggaaacttgt gaaattagct attttaaaac
     2821 cccaccaaac cgcacattga agcttctgag tatttgacaa gtttttccct ctgctatcaa
     2881 gatccccgta ggcttcgggt tatttattta agtaccattc gcttatatcc cgaaaaacca
     2941 tgagctccct aggggcccta attccgtgaa ccctttcgtt agcaataagg gatgatgtcc
     3001 tatagtacaa gctgaggatc ggcagctcgt caaccaaaat ttcttgcact tccctatatg
     3061 catctttgaa ttctgacgga tcatcggtct gggctattcg gtctaataaa tcatccaact
     3121 cttcgttact atatccggca taattattaa atccatccgg aatatccgat gaataaaaca
     3181 aacgtctgag atcaatggcc atatcggaat agtattctac gaataccgcc tcaaagtctc
     3241 cttcctcgag ggctataata tactcctccc aaggtaagag tctgattata acttctatcc
     3301 ccacttcctc caattgtttt gcaattagat ttacagcctc cttatgcaag atattgtcca
     3361 tatgaacatt aatggtaaat gtggggtgca gggtctcctc atcatcgtca tccgaaatgt
     3421 catcataaga atccacccat ccggcctcgg caaggagttg tcgcgctttt tgtggttgat
     3481 ggtcatacat ccttaaatca gaatcataat accaggcatc cgggggtatg ggggcatcaa
     3541 ccgcttgggc attaccggaa tataccctgg ggataatgtc ctttctgtct attccatagg
     3601 ctattgcttg tctgatatcg ctatctctca gtatcccggg aaaattatga ttgataccga
     3661 tgtagttgaa atgctgggtc agataagggt atgattgggc ctgaccctcc gtcccgtagg
     3721 attttccgta tattacatgg gtatccacca ggtccacttg ccccttctta aaagcctcca
     3781 aagattgtac attatcctca tacaccttaa caataatgct atcaatataa ggctttgata
     3841 taccccacca atcatcattt cttattaact taacctgttc accaatttcc agactgccgg
     3901 agtctacttt atatagaccc gtcccgttga gggattctat ttcatccgga tcgaaatcaa
     3961 actccccctc atcggcaggc agtataggaa aagtcatggc ttccaacagc cccctccctg
     4021 ccggctcatt tagcaccaaa tccactgcat aaggattatt tatgacaaat tccatgcgtg
     4081 cgacgctcct gtcgtcaaat aatcttcttg catagacgga ccctggatct tgtccttcca
     4141 actcctgcgc cagattaaac aacatatcaa aggtgaattt tacgtccttg gctgttagtt
     4201 cattcccatg atgccagcta acccccctgc ggagttggaa ccgccataat ttccctccat
     4261 cggtcacttc ccatgattcg gctaacatcg gaaccaagtt caggtcctta tcatacgaaa
     4321 acaggccttc gtagataagc cccgagagat taatgaaatc ccttgatttg gttaaaagcg
     4381 gattaatact ctcgggcaat gaaacaggaa ttctcagttg accccctgcc tccggtaacg
     4441 taggctgagg ggacgagcct atttcctctt tgggatcttc ttggatttca tcctgcggta
     4501 tcggcaaaat gtttgacata ctgcacccca ttatagaaaa tagcatggag aatgtaacta
     4561 agctgtataa gattttgcgt atcattgggg catttccttc ccgttaaatt tcatatagtt
     4621 ttttatattg tttagaggca tcatctaccc tctcaagata atccctggtc tccttaaaag
     4681 gtatactctt aaggtgcctt ccgtcatcgc tgtatctgtc atctttaagc catttcttaa
     4741 cgttgcccat cccgccgtta taggatgcca aggccaactc cacatcgccg tcaaacaacc
     4801 ccaacaaata ggcaaaatac cagcagccga agcgaatatt ggtctccgga tcatagaggc
     4861 tctcctcgtc atattcatcc atacctatct gatctgcgat ccacttacct gtgttaggaa
     4921 tgatttgcat aaggcctttg gcgtccttat tggatgtagc ctcgggaaca aacttgctct
     4981 ctacccatat aatccccatg accaaatgtg gatccagatc atattgccgg gaaaactcca
     5041 atatctcttc ctcatacttt aacggataga ttcttttttg cattatggga gattttaaaa
     5101 accagtatat aaaaacaatt cctattaaaa acagtagcac aacccaatat aaaattttct
     5161 tcatggtgtt ccccgctatc ccttcactac ctatatattt ctttcagagt ctggttccag
     5221 gctctatcca cctgactgcg caggttatcc atatcggagt cattaagaat aatcctgtcc
     5281 gccttagcgt tcttatcctc ttggctcatc tggctgtcca ttcgttttaa cgccccatcc
     5341 ctagataggc cgtcccgatc cataagcctt gttattctca tctccaaggg tgcatcgacc
     5401 acccatactt catccgctat atcatcccat cccacttcaa gcaatagagc tgcatcaatt
     5461 actacgattc ccgtgtagtt ctggattttt agttcgtcaa gcctgtctat tatattgttt
     5521 cttatggcag gatggacgat atgattcagt ctttgcagtg catgcctatg ggaaaacacc
     5581 aattccccca actttcttcg gtttagctcc ccgtctgcct gaaaatatcc cagcccgaat
     5641 tcgtccctta tctttttcca tgccgggctc tcctttgcta ctatttccct ggctataata
     5701 tcggcgtcga tgaccttagc cccaagcttg gataaaattt ttgacacccg agatttcccg
     5761 gcggcaattc cacccgttaa tccaataatt ctcattatag cctccaattt gccctccatc
     5821 tgcaccttct gtaaatttat aaaatgttta cttgggaaat taaagcccgg taggtctatt
     5881 ttacatcgta ccagctggac cctaccccaa tatccacaat taatggaacg gacagctcca
     5941 tagcattttc catcttgtcc ctcagtatct cctttacctc ttccaattcg ggtctgtaag
     6001 catcgataat caattcgtca tggacctgga gtaccagctt tgatctcaga cctcttgact
     6061 ttagttcata ataaacatca ttcatggcct ttttgataat atccgctgca gtcccctgga
     6121 tcggagtgtt catggcaatc ctttccccga aggatcgaat attatagttt ctagaagata
     6181 actccggaag gttgcgtcgt cttcccataa gggttttaat atagccctgt tccttgccct
     6241 tctctaccac attatccata tactccttta taccgggatg cctctttaaa tagctctcta
     6301 tatataggcg agccttattc ctcgatattc ctagatttct ggccagacca aagtcgctta
     6361 ttccgtaaac tattccaaag ttcactgcct ttgcatcatt tcgctgttcc gatgttactt
     6421 cctctagggg tattcccagt acctcagccg ccgtcctgcg atgaatatcc tgattgtttt
     6481 caaacccatg gatgaaggta ggatcctttg atatatgggc taatacccgc agctctatct
     6541 gggaatagtc tgcgtctaat aggacatgat cctcatcgct gggcacaaaa acccttctta
     6601 tcctccgacc catctccatg cgtacgggaa tgttttgaag gttgggctcg gtactgctaa
     6661 ttctgcccgt agccgttata gtctgattaa agctagagtg gattctgccg tccttagggt
     6721 caatcacatt caagagtcca tctacatagg tggattttag tttcattacc tgcctatatt
     6781 ccattatctc tccgataatc tcatgttgat cctttagctg ctccaaaact tctatattgg
     6841 tggagtagcc ggtttttgtc ctcttaacga tgggcaatcc cagcttttca aaaagtatct
     6901 cacccaattg tttaggcgaa ttgatattga attcctgacc cgctatttca tatatactct
     6961 tggtcagccc ctccaagatg gccgtaaatt ctacatccaa ttccctgagg atttcaccgt
     7021 taactttgaa acccgctaac tccatatccg ccagtacctg aatcaatgga tgctcgacct
     7081 cattatagag atcatccata cccacctctt ccagcctctt ctttagatca tgggccaata
     7141 ataacagatc tgctgcgtct acaatatcaa cttctattcc aaggtactcg tggagcaggt
     7201 atttcacatc atatctggtg tgggtaggat ccaacagata ggccgctata aaggtatcaa
     7261 aggatagatt attcaattct atattgagct ttgaagcctc taataacagt ttcttcccgt
     7321 catgaacaat ctttcctatc tctcgggcct tgaggatggg ctctagggcc ttcaatactt
     7381 cgtaatagtc cagccctcca tctaacatat ccctaaatag tttaacgtag tagacctgtc
     7441 tcttgtccca ggagaaggta atggcatcat catcaaatat gaaggcgatc ctccccttat
     7501 taagcccctt ggctacccga gcttgcagcc catccaaggt cttaacctct atagtctcct
     7561 ttacccgctc taaaacttct agtgatggcc cctggtcatc taaatccaat ttattaataa
     7621 tactggtaaa ttccagctcc tccagtatcc cttttaattc cgcagatttg ggcatatcat
     7681 aacagcagtc ctccaaatta aaatcaatgg gcacatccgt tataatggtt gctaattctt
     7741 tgctgaggaa tgcctgttcc ctgtataggg tcaggttctc ctttaatctc tttccggaga
     7801 tctgatcaac gttatccaaa actaagtcta aggttggata ttggctaaga agcttgaggg
     7861 cagtcttctc acctaccccg ggaaccccgg gtatattgtc tgagctatcc cccatgaggg
     7921 ccttaaaatc gatcatctgg tcaggcctta acccgtagac cttttctacc tccttgggat
     7981 cgaagatatg catatcagtg gtgcccctac gatttagcaa aactgtggca tcatgggata
     8041 ccaactgcat agcatctctg tccccggtaa ccagatatgc atgaaaatcc tggttttgtg
     8101 ccaatctgga aaaggtccct aaaatatcgt ctgcctcgaa gccttccatt tcatatacgg
     8161 ctatgttcat ggcttttagc aaagtcttca ataatggaaa ctgaggcagc aattcctcag
     8221 gggtcttttg gcgattcgcc ttatagtcct catatatctc gtgcctaaag gtaggtccct
     8281 ttaaatcaaa ggccacccct atatagtcgg gatgatattg ttctattaag ttgagcagca
     8341 tattggtaaa gccaaacact gcattggtaa acaccccttt actggtggtt aaagcccgaa
     8401 taccataaaa ggccctatac atcaaactgt taccgtctat gattatcatt ttctttttat
     8461 ccacagatca aattcctctc atcataagca ttatccataa cttacctatc gctttcctat
     8521 gcccgcctgc ttgaaaaaca agcccgaggc tagacaccat caactttaca ctatgattct
     8581 atcatatcac gtcttcaaat ccaatcacag tgttttacca aattttgaac agtgcatctt
     8641 tgttacaaat gtgttaagat gtattacaaa tgatatacaa gttattacta caatgtttaa
     8701 caaattactt ttcaggaggt aaatttatga ggaaatcatt agtagctttt atattaactt
     8761 taaccctggc tttcagcctg ttatcaacac ctgctatggc atattacggc agggattgga
     8821 accaaggtca gcatgttaac aattacatca attcgggtag cgggtacagg tcgattccct
     8881 tctttcgtca agatacaaac ggaatgctac ttaggtttaa caattggtat gtgaactcct
     8941 ttaacagagt atatcaatcg ggatccggcg ggaacattca agcgccgcag ccggaaacaa
     9001 gacccgaagc acccgctccg gcacctgaag caaaaccgga acagccggcg cctgctcctg
     9061 agtacaaccc ggggcctacg caacagccgt cacaacccac cccggcgcca caacccgaag
     9121 caaggcccga gcagcctgtc cctgcaccac agccagctcc tacaccacaa ggcataggcg
     9181 gacaacaagg tcaaatgctg gatatggtaa accaggagag aataaaggcc ggtttaaagc
     9241 ccctagcatg ggactctgac ttggccaatg tagcacaggt taaagctaag gatatgagcg
     9301 acaataacta ctttgatcat aactcaccta cctacggaag tccttttgag atgatgaaaa
     9361 agtttggcat aacctacaga gcagcagggg aaaacctggc aggatctggc ggcgtggaca
     9421 gagcccatgt cgggcttatg aattccgaag gtcatagaaa gaatatccta aaccccaact
     9481 ttacacattt ggggatcggg gttgaaaaga gcacccgcta tggctacgta tatgtccaga
     9541 tgtttgtagg caggtaatag gacgaatagc acccgagata gaagattatt taagcttatt
     9601 taggcttatt caaaacagat agactccttg ttaaaggcga tgatactcgg tatcatcgcc
     9661 ttttgttcgt gatccaaaaa ggatattcaa ttacaaaacg atcagacaat ccggccttta
     9721 tatttatcaa tcccaaattg caaggattaa tcttaccgca ccttatatat tatggttata
     9781 tgggcctcta tttccagctc accggaagct atgggaaccc caccgttact gtccatctcg
     9841 tcttccacaa aatttattcc agtcataccc ctgggataaa tctcattggg tgtatttccc
     9901 tcatagacag ccaatggctt ttctaagata acgcccgcct tttttgccat tacttccgcc
     9961 ttggccttcg ccttatccat cgccttttcc agtgcctctt cgtagacggc agcggtgtcc
    10021 agtatcccga agcttattcc ataggaccta ttggcccctt ccctggagac tgcatccaac
    10081 acctccccta cagaatcaat tttacggacg gtaaccctaa gcatattttc cacttcataa
    10141 ccctctaaaa tctccctgtt gccattatag ttgtacctag gccatatatt ataagctgat
    10201 gtctgaatgt ccttatcttc gatgttcagc gaagccagtt ttcccacgat cttggtcatt
    10261 atatccttgt tctcatcctg tgctattttg gcatccttat ctactgtcct gacccctact
    10321 tccaagtagg caacatcggg ctttatgctg acctttcccg tccctgtcac tgttagggta
    10381 ttatcccttg ccctgtcatc aaccctgtcg gtgcttaagt tcacctctga tccggcgact
    10441 ctcccggcag taattatgcc ggttattgct accgcaccta tcacaagcag ggtacccacc
    10501 agtaaataga taagtttttt attatccatc acggtcactc cttccctttc tgatccgcct
    10561 actttttaac catgccccca gcagccatat tatccagcca aggggaatca ataaaacgaa
    10621 aaacggcaag gcgcctgcca ggaatatagt catattctcc aagaatcttc ccatcccctt
    10681 taatgaatcc ttaaaggcat gggctatccg ttcccctagg gtctcgggct ccggttcagt
    10741 aggctttatc tccttgacct catacacatc tacattgacc cgtgagaaat tgaccatttt
    10801 ctcaagccct tttaaggtgc ccgtatagtt ttcaatctcg taccttaccc ttgagagttc
    10861 gctctccaat tctataatat cctgcaggat ctccgccctt tcgagtatgc tgagaagcct
    10921 ctcttcctgg attgtaaggg atttgaccct ggcctgggta tccagatact gccctgttac
    10981 atcctcgccg ctgatttcct tggttataag attgcccagc tcccctacct cattattgaa
    11041 ttgttcaaac tttttgttgg ggattctgat ctcataatta gcttgtctgg gacgaaaact
    11101 acccatggtt tccttggata tcccctggat attggaggat tcaatatatc cccccatgag
    11161 ctttaccttt ccttcgagat ttctggtaaa tacatcaaat tccagggttt ctatgcccag
    11221 ataagcggct ttgataatca ttcgttgggc attacttata tgttctttgc ccatatcata
    11281 ggatatatca ctgtcgtcac tcccccatcc tacatcgctc ttttggtcca tttccccctg
    11341 ctccatactg ggcatttcca tatagtcttc ttcgctttca acatatccgt ctgcctgtgg
    11401 ggcctccggt tgcctatttg attggctttg ggctaggtca taggctgggc tttgagattt
    11461 catccatccc aatcccaaag tgtttaccat ggtaatccct attgctataa ccaccaatac
    11521 cgaagccaat ccaatgaata ttggcaccct gtgtttcctt cctaacattt cattctcccc
    11581 ctttttatat aacttttcgc tcagtctgct atgaaaaccc ggaggtactt ctacggtgcc
    11641 cagctcctta caagagttct tgattttcat aacgtcctcg tagtattctc tgcaatcagg
    11701 gcaccgatta aggtgggcct ctactttatt aagatcccgg ccaaccagcc ggcggtctat
    11761 atacagtgaa agttcatatc tgattttctt gcagcccatc cttattcgcc tcctgttttt
    11821 gcttcctata tctattgacg ctatatatgt taaatagttg caaaaaaaat tcctgtctgc
    11881 gatcagacag gaacctttat ttccctttgc ttgttataag gctgaaacgc cgaatatata
    11941 ggttcaaatt gggagttaaa gagcgcagta gcgtggtgta gatagcttcc cttaacataa
    12001 tatcttagat cagtgataat agctctatga tttttcccgt cctttatcca gtccacatga
    12061 tagtggggag aaaagtcttt aaaaaatcgg cctatattgg tatccatcac caaactttcg
    12121 agccccgaac tgctccgcct taagcgatcc ctcacctcga tatcgcccct taaggtattg
    12181 acccgtcctg ttatgacctc tgcactcttg tacaggataa agtcccattt aaagagcctc
    12241 agcatagagg gcatgacaac tatctcctga tccgcttcat tcccaaaata tcgaatcaaa
    12301 tttttgtata cccgtctctt gagtaaccat ctaaatatga gatacagggc aaagaacact
    12361 atactgccca ctattcgtcc catatgcgcc cccttggaaa atgatattat tccggccgaa
    12421 atcagcaggg cgggatcaaa gatcaacaac aaacccgact caattttctt acgatttaag
    12481 ggccatagga tatttgcgcc gtaagaatta aaaacatcca atcctatatg aagcatatag
    12541 cctccaaagg tccaggcaaa gatactccaa aaagaaaaat catgaaataa tcccctgagg
    12601 atcccggaaa tcatcaatga tattatcgtt cccatggcta tagaatggga cggtcccctg
    12661 tggtgcttta aataggtgta gtcccccttc aactggtaga ttatatcgcc gtcgggtatt
    12721 acagagccta tgagactgcc tatagccaat gggttattta ttatgccggg caccccggtt
    12781 aagctgccaa gcgccaggcc tacaacccca tgtgttatca aatccatgtt atcacctttc
    12841 atttgcttta cgataaacat actatattaa tgcactgtac cggttaaaca cccaaatgaa
    12901 ccggcatcac agattacatt atgcttctgg catcataaaa catcattact tatatgctgc
    12961 ttatatctat atgctgcgaa aaatgcgtcg atccaaggca ggatcaaagg gctcaatcga
    13021 ccttgaaccg ctagatatgt tacgaaaaag atctttcaat acaaaatcag ttaagttatc
    13081 tgattttgtg ttaaagcaat gtatttttcg taacatatga attgaggata atgttttacg
    13141 acacccctat gctaatgtag aatgtttgtc gtaaaatata gacccccatg gaacgtacat
    13201 agtttacata atatattttt acagacatga tacgattata acatggactt ataggcgtag
    13261 gcaacaacaa tatgttttac gacaaacatt acctatattg gtacacaggt ctgctgtctc
    13321 aaaaaagttg ttgcatttct tcacaggtta tgctaatata tttttactgc gtcccacggt
    13381 acggatattt gcccgagtga tggaattggc agacgtgcgg gattcaaaat cccgtggtag
    13441 cgataccgtg tcggttcgag cccgaccttg ggcaccaaaa taaaaatcct ataggctcaa
    13501 tgagcttata ggattttttt ctgtctccgg ttctactctt ccacttcatc ctcctgccct
    13561 aacaatctgg gtctgggaca tggttttgga tctatcggca tatcctccag ctgtggcgga
    13621 aataaaggta gtctgaagaa ttcgtcgcat acgttggatg cgaattcttc acaaggagga
    13681 atctcgcaga aaccgaatgc gggaattaac agttcgacct tcgcaataat cttgagtatg
    13741 atggttatgc atacatccag atcaaatcta aatgcatcgc cgggtataaa catcccagaa
    13801 acacagatgg cgttgacaac gctttccagt tggaatggga ttactgattc atccggcaca
    13861 aacattatta catccttagg tatacatata acggccctac cgaccccctg ttttcccctt
    13921 gaatcctcaa acacaacttc gatgggtatt tctactttag tcctgactct ggcaaagttc
    13981 ggtctgtctt ccagcctttg gattacggtt cctaccagct tacccgtgac cgaagtgctc
    14041 ctgcagctga ccagggtaag gggcggatca aacccggaac cctttacatg ggtaagattc
    14101 accctaacat tttctagaac ttcctgttgc aggcaagaat catagacctt gttgacttga
    14161 atacaaaccc gttccttaat cctttttaac ccatcgaaac caattaacga aggacaacgt
    14221 tcattcccct gattttgctg agaataaaaa gacatcttat aaccccctca aataagtggt
    14281 ctcatatata agagcatgtg cgctccttat attgcatagt atgtatggga agcagctttt
    14341 gtgaataagg aagtttctaa aatgtttttg caatttgtta tagataaatt tagttgacct
    14401 attgtttatc tacagataat caactacaac tataaaaggg gaaccattcc tacaatggat
    14461 ttttggggag gttacagtca tggatgggat tattaacttt gaatcgacta gttttctaca
    14521 ggatcgggaa gggaccatat ggttcttcta tttgtccgat aatcaaagaa ttgggtatgc
    14581 tgtcaatatt cagggcgatt ggtctgccca tgaacatatc gattctcagc ccataaaggg
    14641 ttattcggcg accctagaca gcaaaggcat tatccgattg attgcctata ccgctactca
    14701 tcaattaatc tattacgaat tcgtgggggg taaatggaat agccaggtga tagagcgaat
    14761 ctattccaga tatcaggata tcccctatta ttctatacga tcttcggtat tgggcattca
    14821 tattctgtat tacataaatc attccctcag cagaagcggt gaaaccctga tccattacta
    14881 tctgcaagat gggagatgga acggcggaag gctctggaaa tttgtatcgg accagatgac
    14941 tatggtccgg gatctatata tcgattccca taacaacctc cagcttctat tcacccaaag
    15001 atggagacaa aaacaccatc tttatcactg tgtatacgac catgccacct tatcctggat
    15061 ggatcctgtc aatatccatt ccgcggtctc tagctatgac tacaggttgt ttgtaggttc
    15121 gtcagatgat ccccacatcc tctggcatac agaaatggag ggaaaccgac atatacacca
    15181 cctgtttaaa tcggggccac cacaagatag cccctcatgg caggaagcat tgattcatga
    15241 gaccccacac agcattgata caccggtgct catggaagac gataatgggc tgtattgtat
    15301 ctggaaggag gatggtctaa tatacaaaag gctttcacat gatcttggga gaacctggtc
    15361 tacccctcaa gtggccacag acagcatgaa ttatgacgcc agcctccttg attttgcata
    15421 cttagacaac ggtataccca gatccctaag cctatggggg cagggctacc cggctatgag
    15481 acttgcagta gccgacgata agatagtgat agatagggta aagatgcaag attcaccaaa
    15541 acctccgcaa ccctcaccgg atcttgacca gggaaagagg gattcattgg ctattgctga
    15601 tattaaaaga acaatcgctt ccataaggag aaaaaacagc ggtctttcgg aggacattgg
    15661 gaatctgtct gtccaggtgg atcgtctaaa tgcattggtc tacacccttc aggagcagat
    15721 acagataaat gaccgctcac ttttcaatat ccatgcacag attaagcagt tagactttca
    15781 gataaaacag cttcagctta ggtctcgtca ggtaagcccc agggtgtata gtgcggacga
    15841 tagccgatat agacctttgc ccattacctt ggatcaggat ggaaaaatcc ttgtagaatc
    15901 tcagcatcta ccggcagatg tatcggcgga tgtagaggag gacgaaggaa tggagccgtt
    15961 ggaggaaaat gaaagcaaat taattcataa agaggattct aaccccctaa ataacaatca
    16021 gacttacgcg gaatcgaaat catctccaaa ggtaaaaccg ccgcaaacag gtgattcaac
    16081 cggggggccg aaaaaggatc cccctatggg gggtgggaac accccaaagg aaaccaaacc
    16141 caaagtccat ggtgatggtc ccgtagtcag cgaaagtacg ggagatagca taagtatgga
    16201 agatagcata agtacggagg ataggaaaag tatgggggat aggaaaagca ccaaggaaag
    16261 caccagccca agggctatag tcaccgtcga ggccgtagta agtcccgaag atgacgatcc
    16321 cccggaaacc gatagccatc cggaagataa aagcaacagg gatattgaaa ggatatccct
    16381 aggcaatacc actatactta taaaccctga aaatccagag gatttttaaa aagagaatcc
    16441 agggatttta aaaggacttc cctcccctgc ccggtactcg aggaaaatgg aatcattgga
    16501 ccatctgccg aaagtcccaa tttgttccgc aacttgtcaa tttgcggttt gattctggtt
    16561 tttcccagtt tatcggcctt tgtagccacg atgattagtg gcatatcata gtgacctatc
    16621 caattgagca tagtaatatc atcatcggtg gggttatgtc ttatatctat gagttggatt
    16681 attcccaaga gatttgggga ggcagagaga aacgtctcaa tcatatatcc ccatctctcc
    16741 ttttcttttc gggacaccct ggcaaaccca taacccggaa gatccaccag atagaaagtc
    16801 tcattaatcc tataaaaatt taccaaccgg gtcttccccg gctgaccgct ggtcttggcc
    16861 agcttagacc gcctagtaat tgtgtttata acggaagact tgcccacatt agattttccc
    16921 gccaaagcga tgtagggcag atgatccgtc gggtattgat gcgattctac ggcactggtt
    16981 ataaattcag cctttactat ctccatctct atccccttcc ctccctgata gcgcatgttc
    17041 taggatctca tccatatttt gaaccgctac tatctctatt tcttccttaa tgttatccgg
    17101 tatctcctct aagtccttgg tgttctcatc gggaattata actttggcaa tccccgccct
    17161 atgtgctgcc aagatttttt ctttgagccc ccctatgggc aataccctgc cccttagggt
    17221 aatctcgcct gtcatcgcta tgtcccgcct taccggcgtt tccgttaggg cggaaataat
    17281 ggcggtggct atggtaatac ccgccgacgg gccgtctttg ggaatagctc cttcgggtat
    17341 gtggatatgg atgtccgtat tattataaaa atccggatct atatcaaaat ctttggcttt
    17401 ggatctaata taactgagac ctgccctggc agactccttc attacatctc ccaattgccc
    17461 cgtcaaaacc agctttccgg tccctaccat gggagttacc tctatataga gggtatcccc
    17521 gcccacagcc gtccatgcga gcccggtaac caaaccaacc tcgtccttct ggtttgcctt
    17581 attaaagcta aagcgtggga tccccagata ttcatgaagg ttttttggcg taattcgaat
    17641 tcgctttctg ccggcctcca ataggaacct tacccctttt ctgcagatgg cagctatctg
    17701 tctttccagg tcccttaccc cagcttctct ggtatagtga ttgataatgc ttcggatggt
    17761 ttgttcagga aatatgatgt tccctctctt taatccgtgc tcctccagct gctttggtat
    17821 tagatatttt aatgcaatat ttgttttctc ctcctctgtg taccctgcga tgcgtattac
    17881 ctccatcctg tccaacagcg ggcgaggtat cgtatctaag gtattggcgg tggttaaaaa
    17941 cataaccttt gacagatcat aggccagttc cagataatga tccctaaagg taaagttttg
    18001 ttccggatct aatacttcca gcatggcaga cgccggatct cccctaaaat cgccgctcat
    18061 cttatctatc tcgtccaata taaagaccgg attttttgat cccgcctgtt taatggagga
    18121 tattatagtg cccggtatag aacctacgta ggtacgccta tgtcccctga tttcggcttc
    18181 gtcccggacg ccgcccaatg acatgcgcac aaagtttcgg ccaagagccc tggcaatgga
    18241 ttttgcgata gaggtcttcc ccacccccgg cggccctaca aaacagagta tggggccttt
    18301 catattctga gtaagctgca ggacagccaa atactccatt accctctctt ttaccttgtc
    18361 cagtccataa tgatcctgat ccaaaatgcg cgccgcagac tttaggttat ggttatcctt
    18421 ggtctcgtga ttccatggca aatccaatac ccagtccaaa taggtacgga taatggaact
    18481 ttccggcgag gttaaaggtg tcctcgaaaa acgctccaat tcacttacgg ccttctcatg
    18541 gacctcctca ggtagtgaag ccttttctat cctcttgtta tagtcctcaa cgtcagagga
    18601 tatactgtca ctttccccta attctttttg gatagccttc atctgctccc taagatagta
    18661 ttccctttgg actttatcga tctgtttttt aaccctgagg ttgatcttct tttcgatcct
    18721 cagtatttga atctcgtttt ccaaaaattt atgcaatatc tgaagccttt ccatgggcct
    18781 gaaggcttca aggatctcct gtttattact gtttttcact aacacattgg cagctactat
    18841 atcggcaaat tggcctatat cgtctactgt attaaccgat accagagtat ccggggatat
    18901 cttattgctc agcttgataa attcttcaaa ggcatccatt acggttctca tcaatgcctc
    18961 ttcttctaag ggatcgctat cctcctggtc cggttgttcc tctataagta cctcaaagaa
    19021 cttatcgttg tctaaaaact ctacaatcct gcccctgttt ataccctcaa ccaatactct
    19081 gatggtatct cccggcagtt taagtagctg ttttatctta gataccgtcc ctacggtaaa
    19141 taggctatcc ttgtcaggct cttcgacctc tggttccttt tgagttacta aaaatatatc
    19201 ctgattattt accattgcat cttccaatgc tgagatggat ttttgtctgc ctacatcaaa
    19261 atgtaggacc atatatggaa aaaccgttag accccgaagg ggtaataggg gcatttgtct
    19321 ggtttttatg tctttctttg acatctttca ttgctccttt ctatatgctt cacgacaaac
    19381 attctacatt aatatgccgg tgtcgtaaaa catcatgctc aattcatatg ttgcgaagaa
    19441 tatattgtcc ccacacaaga tcagatgatt taactgatct tgtaatgaaa ggtcttttcg
    19501 taacatatgg agataacagt ctatatggga tgaaccaaaa cgcacttagc atgaatccgg
    19561 aaaactatcg ggtatcatca aataaacatt gtgtagtcaa agtccaaaaa gttgaattat
    19621 ccataccgtg gatcggcatc gtataatata ttttttgaaa cttcctcgat gaggccatta
    19681 tatactcaac aataaatcgg tgcaaggcaa aaaaatgccg gcagtgtgcc cggcatttaa
    19741 atatcaatta gaaaagccaa gttaagttgt ataatgcctt cttccggagg cgctgaggag
    19801 acccgcattc tgtatcatgg gttctaaatt ttgctcctcc cgccctttgc acaccacctg
    19861 ttctatgacc tcttctattc ttctaaccgg tattatctct ataccgtatg ccgagtgctg
    19921 ttcattccaa ttgtccttag ggatgattac cttcttgaca ccggccccta ccgccgcatg
    19981 cagctttgca ttgatacctc ccaccggctt aacctctccc cgaatggata tctcaccagt
    20041 catggccgaa ttgttgtcga tgggaagacc cgttatggcc gaataaatag cgattactat
    20101 gcttattccc gctgacggcc cgtcaatggg aatccccccg ggaaaattca aatgtatgtc
    20161 ataatccctt ggatccacgt ccaggaactt ccttatcacg gtcattacgt tatctactga
    20221 actcttagca gtactcttac gcctgctttt tttgactcca gaaccgattt cttcctcgtc
    20281 gacaattccg gtgatggtga caataccgcc gcctttaatg cttggcaggg ccgatgcctc
    20341 cacctcgatt aaggtcccta ggttagcgcc atataccgcc agaccgttaa catatcctac
    20401 ttgaggagaa tccagcacct ttttttcggg tcggggcgaa taatgtccgc tgtttatcac
    20461 ccattcaaca tcctccacca gtatatgatt ccggttttct gtcagggcca agcccacggc
    20521 taattgaatt atatttacgg tatcgcgtcc gttggatgca tatttaccta ttagctccac
    20581 actgtcctca cccatatgga acccggcctt atcagcggca tttcgggcta ttatcatatt
    20641 ctcttcctgg gttaagcccc taaaatagat ttcaaggcac ctggacctga tggccgggga
    20701 tatctcctcg ggatttctgg tcgtagctcc gaccaatcta aaatctgcgg gtaatcccct
    20761 tttaaagatt tcatgtatgt gccttggaat attgctgtct tccgagttat aatacgcgct
    20821 ttctaaaaaa actttgcggt cttccaatac cttaaggagc ttattcattt ggattggatg
    20881 taattcgcct atttcatcaa gaaatagcac gccgccatgg gctttggtga cagctccggg
    20941 ctttggctgc ggtatgccgg ctacccccaa cgggccggct ccctggtaga tcgggtcatg
    21001 caccgaacca attagcggat ctgcgatgct ccgctcatca aatcggacgg aagtggcatc
    21061 catctctata aattttgcat cctttcgaaa cggtgacatg ggattcttct ttgcctcgtc
    21121 taagacgatg cgagcggcac atgttttgcc tacaccaggc ggcccgtata tcaatatatg
    21181 ctggggatta ggtccgcaca gcgccgcctt taaggcttta ataccctctt cctgtccgac
    21241 tatatcgtta aagacgttgg gccttgtctt ttcggacagg ggttcactga gggagatctc
    21301 cctgagcctt tggagtctct ccagctcttt tcgcgattcc ctttctacgg ccgacttgtt
    21361 tccctgttgc ccctttaata gattaagaaa atacagacct atcacaacag caaagaatag
    21421 gtttataaca gccaatatat ttcccagcaa aacactgcct ccttacaaaa gtataacctt
    21481 tagatccgcc gaaaacatgc aaattctcgc tatgacagcg gtggcgaccc tgccgcaatg
    21541 gctctatttt accgcataaa gaaagcgttt atacatggaa gccatttata gcaggcaaag
    21601 caattgctac ggcaattgca tcaggctggt ttaagcaggg cgggaatata tgcgcacccc
    21661 ctgttgtaca aatcatagcc caatacttat gggagtgaga aacatattac cgccccgtaa
    21721 tattatggtg taaataagtg gtttttatcc cataaggctg ccgggaagta ttcccataaa
    21781 aaaggagccg tactaggctc cctttaagat actgattctc tcttatcctt tgtctttttc
    21841 ataccctttc ggcttctctg gccttcggat agaattagta taggatcgct attgttaatt
    21901 atggtttcct tggtaattat gcatttctcg acatcatccc tggaggggat atcgaacata
    21961 acattcagca taatcctttc aagaattgct ctaagacccc ttgcgcccgt cttcctctcg
    22021 acggctcttt ttgcgataag cctaagtgct tcatcctcaa attccagtgc gaccccatcc
    22081 atctccaaca gcttctgata ttgttttacc aatgcgttct tgggctcctt taggatttgg
    22141 accagcgccc cctcgtccag ggaattcagc gtaacaacca cggggatacg acccacaaac
    22201 tccggtatca atccatatcg gagcaggtct tccggcaaaa tcttttccaa agtctcgcct
    22261 acgtcgtagt ttacgttgct acgtatctcg gccccgaagc ccattgattt tttgcctatc
    22321 ctgttttgta ttatcttgtc tataccgtca aaggctccgc cacaaataaa gagaatattg
    22381 gtagtatcaa tctgaatgaa ttcctgatga gggtgctttc tccccccctg gggaggtaca
    22441 ctggcaacag tcccttccag aatctttaga agagcctgtt ggaccccttc cccggaaacg
    22501 tcccttgtta tggagggatt ttcagatttt ctggctacct tatcaatctc gtctatatag
    22561 ataatgccct tttctgccct ttcaacatcg tagtcagccg cttgtataag ctttaatagg
    22621 atattctcca catcctcacc gacatatcct gcctctgtga gggatgtcgc atcggcaact
    22681 gcaaaaggta catttaaaat tcttgccaag gtctgggcta ggagagtctt gccggagccg
    22741 gtgggtccaa gcattacaat attgctcttg tagagctcaa catcatcctt tgccacgtca
    22801 ctgtttatcc tcttgtagtg attgtatacc gccaccgaca aggtcttttt agcagcttcc
    22861 tggtcaatca cgtattgatc aaggatttcc ctgatctcta tgggttttgg gatgtccctg
    22921 atatccagat cagaggcgtc atcgaattct tcctctatta tttcttgaca cagctcaata
    22981 cattcatcgc atatatagac cccggggccg gctaccaaac gtctgacctg atcttgggtc
    23041 ttgctgcaaa atgaacactt gagttgcttt ttgtcatcat atctagccat actttcacct
    23101 cttctttaac tatatgtttc gcattaaata ttgtgtcaga ttatttcctt atcgcgaacc
    23161 ataccgttct ttaatagagt gttctgtatc agtaatgtgt gtatgcggac aacttaaagt
    23221 cgcccttgcc aatctatgcc aatctatggc ctttccttac atttcggatg ttaaaaatca
    23281 tcccgtctcc gccataatat tatcatatcg gaggattaaa agctaacctc atacatttat
    23341 ttacgggaga caattacatc gtcaataatt ccatagctct tagcgtcctc agctgacatg
    23401 aaaaagtcac gatccgtatc cttttcaatc ctatccaagg gcttgcctgt tctttcggct
    23461 aagatttcat ttatcttttt ctttagcttc agaatctgtt cggcctggat cgcaatatca
    23521 gaggcttggc cctgtgcgcc gcctagaggc tggtggatca tgatctcact gttaggaagc
    23581 gccaaacgtt tgcccttggt tcctgcagcc agtaaaaaag cgcccatgga tgcggccatc
    23641 cctacacaga tggtagacac atcgcatttt aggtactgca tagtatcaaa tatcgccatc
    23701 ccggcagtga ctgatccccc cggactgttg atatagatga gtacatcctt atccggatcc
    23761 tttgcctcta aaaacaataa ttgggcaaca actaaattgg ccgtcaaatc gcttatagga
    23821 ccacctaaaa atattattcg atcctcaagc agcctggaat atatgtcata agacctctca
    23881 ccacgactgg tttgttcaac aacaataggc actaaattca ttttgccgtt ccctccttat
    23941 atatttcggc ggtttaggta ttggtattcc taaccttcct gtgtctgttg ctcctcgggt
    24001 tttgcatctt cttccggggc ttcaacaatt accgcttgct ccataagaaa atctatggtt
    24061 ttttgtatgg ataggctgtg tctcagccca tcttcttggc cctcgtacct tttctttacc
    24121 tcatcgaggt ccaatttata ttcttcggca atggtgttgt attccttttc cacatcctcg
    24181 tcagttgcct ctattgcctc ttccttattt atctgttcaa gagccagctg aagcctgacc
    24241 ctgtggttcg cctcgtcact catatcggat ctaaagttat ccatagacga gcctatcatg
    24301 cccagatagc tatttaaatc catgccctga tacctcatcc tcgtatccat ttctctgatg
    24361 gatgtatcta tttccctctc aatcatgacc tcgggtacat ccgcttccat attttcaact
    24421 atcttcctaa ggaggaggtt ttccatctcg cttttccctc tgcgtgccgc gtcctcgtcc
    24481 atgcgctctt ttaaatctgc cctatattct tccacggtat caaactcact gatgtccttg
    24541 atgaattcat catctaattc gggcaactct ttttctttta tttcatggac tttaacctcg
    24601 aaaatagcat ccttgccttt taaatcttcg gaatggtatt cctcagggaa agtgacctta
    24661 acttcacctt cactgccggc atccatacca accaactgct cttcaaatcc gggtataaat
    24721 tgccccgaac ctatctctag ggtttggttt tccgcagtgc ccccctcaaa ggcttcaccg
    24781 tcaacatagc ctttataatc aagggttata cgatcgccct cttcaacgga tctgtcttca
    24841 acggttatcc atcttgcatt ctgttcccgg gacctctcaa gctgatcgtc tatatcctgg
    24901 tctgtaacat tatactcaac cttgttaatt tctattccct tatactggcc cagaacaacc
    24961 tcaggtttta ccgctacttc tgcagtaaat ataaggtcct gaccgtcgcc gatctgcaca
    25021 acatcaaagt ccggctgatc cactgggaac agctcatgct cctctacagc ctcatcatat
    25081 atagtaggta aggtttctgc taaggcgtcc tcatagaata ttccttcgcc ataatagcgt
    25141 tcaattatct ggcggggtgc ctttcccttc ctaaacccgg ggatggatat gcgtccccta
    25201 ttttttaaat atgacttttg cattgcctca tcaaactgct ttgcgtctac ctctatttcc
    25261 agtttcacct tgctattttc gagtctttct acattcgctt tcaccaaaaa gtcctcctta
    25321 gttcgtatat accttaagat atcgggagta attgttctaa caattgtata tacagatttc
    25381 aacgaggtgg ggatttttat ccgccgcctg ttttagctaa tcggaatcca ccacttgaag
    25441 aaacagtaat tgtctttccg tcccgttata atctttaata ttataacaca tcattcatgg
    25501 ttgtccaaca ttattatagt gtaatttgct aatcatcgcg taatttacta atattccttt
    25561 gacaatgtct gagagtctcc attataattt atataaccgc ccggaggata aaataacaca
    25621 atcgattcct tgagtgggta atatccaatt taagatgaaa ggaatggcgc aattgaatac
    25681 tttattgaag gataaaacga tcctcatcat gggggtagct aacaaatgga gcattgcatg
    25741 gggtatcgca gaggcctgca tcgatgcagg ggccaatata atttttacat accaggggga
    25801 gagaaaccta tccagccttc aaaagcttac ggcaaatcta cccaatgctt ctttatataa
    25861 gtgcgatgtg acggtggatc aagatattac agacctattc catgatattg gcaacgattt
    25921 tgggactgtc cacggtatag tacatagtat agcttttgca aagaaggaag agttagaggg
    25981 ggcttactac aacaccagcc gggaaggata ccatctggcc caggacatta gcgcctactc
    26041 cctggtggct gtcagcaggc atgccaggcc tcttatgcag gatgggggca gcatccttac
    26101 cttaacctat ttgggaggag agagggccgt ccaaaattat aatgtcatgg gagtggccaa
    26161 atccgcctta gaatcgtcag tcagatacct ggcggctgat ctgggcccgg aaaatattcg
    26221 ggttaattcc atctcagcag gccctattaa gaccttggca gcccaaggcg tcaaagggtt
    26281 tgggtctatc ttaaaggtct atgaggaaaa ggtacccctt cgcaaaaccg ttacccaaag
    26341 ggaagtgggg gatacagccg tctttttgct aagccaaatg tctcgcggca taacggggga
    26401 aaatatccac gtcgatgcgg gttatcatgc catgggtctt ctataacttg tgtgttgcaa
    26461 ttgcttccga tgttataacg ggtcggcaag atgtctcccg cttctataag cgatgagttc
    26521 ctgttattta tttaatgaca cctagaacaa ttcccccaag cattccttga gtatgaaaaa
    26581 gcccattact aaaaacatta ttccggctgc catctttaaa atggcggccg gtatatattt
    26641 gcagacaaaa ccgcccataa aagcacccaa cagggtcact actgtcagcc ctaaagatgc
    26701 acccagaaaa attggccatg gctgcttatg ctgggttaca agcgtaaata ccgccaactg
    26761 agtcttatcc cccagctccg ctacaaacaa caaccaaaat gataggaaaa ataattttaa
    26821 atccattacc ctcacccttt ttcatcgtcc ttggaatcta acagcgtgaa tactgcctac
    26881 ctataacttc atatatcgtt tgggttgttt cccttatcat gtcctctaga cgaaacttag
    26941 tcagaatttc cgcccttcct acctttgcat attcatatgt ccgttgttca ttttccagat
    27001 agaacaatag agcatccgcc aattccccac tatctccggg tggaatcaag tacccgtttc
    27061 gattatgatc aataatttcc cttataccgc caacatttgt tgcgataacc ggcttgccca
    27121 tagccatagc ctccaggatg gagataccca atccttcgga tagggacggg agcacaaaaa
    27181 tatcaaaggc ttcgtaatag taccatatat attcactatg tcccgcaaat ataatgtttt
    27241 tgctgcaatt taaatgggcc gccttatcca ttaggtttga tctatccggg ccgtccccca
    27301 cgatcataaa ctttgtatcg gggaactcct ccaagaccgg cggtaccgca tctattagca
    27361 catctaggcc cttggccgga atcagccttg ctacagttcc caccagtttc tcttccggct
    27421 cgattcccca catctgcctg atatcatgat catcacctat ataattgggg ggatcgatcc
    27481 cgttatagat gatctttatc ttatcgggat cgatggccag ccccttggcg gtatattttt
    27541 ttagggcgcc ggatactgtt attatatttt ttgttattcc ggacaacctt ttttctgccc
    27601 aagcgattcc cattgttccc aatttcccta agcttttggg gaagaagtta tgtatagtgt
    27661 aaatacatgg acaccttaca aacagggctg caatccgtcc tatgagtcct gctctaaatc
    27721 catggcagtg gaggatatca accctatatc ttcttagaag tctggccagc cttaaaatgg
    27781 ccaatatatc ccttatagga ctgattctgc cgcgtatcac aaaggggaca accgttgccc
    27841 cccactttga cagtctcttt ctatcccctc tagacaggct gcaaagggct attacatcca
    27901 tgccgtttcc tattaacccc tctgtaagag ccatgtagtg ctccttcatt cccccctctg
    27961 cccttctaat cagttcggcg accctgggcc ttgaatctga ttccatcttc ccatactccg
    28021 ttcatgactt ttctttggac ctcatcccct atgagtaaca cgaagggcgg cgcaacaaat
    28081 atcatagccg ttgccgcgga tactatcccg gaatcgttga atataagggc tgctacggcc
    28141 ccaattatgg cgcccatcaa tccccttaaa aatatcctgt gcactctata catatcccta
    28201 aaaatcccca caggtcgata gaggagtaaa accataaccc ccagggatgt aaaaagtacc
    28261 cttgtccata tggtggttct aaagagcctg atattcatgg atatcttcct atgaaatatg
    28321 tccagcaatt ccccgagacc gttttctttt acggcgtttg ccgtctggcc catgtggctc
    28381 tgactgtcca caaccctgag ggaatcataa aagaaaattc ccgccagcaa tactatcatt
    28441 atggcagcca tggatattac gctgtgaatt cggatctttc tgttccggat tatcaaagcc
    28501 aaggaggaca gggcggcaaa cattgccatt gtgcccccga cattggcccc taacccaggt
    28561 gacgccaagg taaataatgc taatgatccg gtgatcagca tgaatacaac ggcaaccctt
    28621 ttgcgattta taaacctctg atacaccgct gcaatagtcg tacaagctgc cccgaccata
    28681 acccccatat actcgtttcc catgccatag aacctggccc cggctattac gtcgtaccct
    28741 aagggggacg ccttaatgag ctgcataccc aaccactggt caagcattaa agctccgagg
    28801 gtcaaaccgg agattatcag aaccttatcc atggtagatt taatccatct gcataatatg
    28861 ccggttatta ttacggacag ggatatggct atcagggcgg ttctgtaaag ctgcggcttt
    28921 tggagcatcg gcaaaaccag gtatacaaaa ggcatgacca tgatgtagat tagcatgggc
    28981 tttatatgcc ccaggtattt tctcttaaaa agaagaacag ccgccgccaa acccagaatg
    29041 ggtatgaggg tatatacata tccctttatc aagaagggcc tttggttgta gatgtctaca
    29101 agcccgctat taaatcggac caattcatcc ttccctgtat tacccggcag agaatagatg
    29161 ggggccccgc cttgacccgt caagggcatt aaatcaaaaa agttcaatat actggccccg
    29221 acatctaaat tggttactat tcctcttctg tgggtagaac ccgatgagag ccagccccct
    29281 tggatgcccc gccctgccag gattattggg gtcagcctat tgttatttcc tatttcctta
    29341 gccggaccca ccggtgtaag gaccatcata tagtcgtttt gaagatccgc gtattctaaa
    29401 atcccgccga taaatttatc ccccgcttcc aaggcccttc ttttgtgata ttcaaccctg
    29461 gaatccatgg actggtgacg aaagtcctca gctctggatg tatcccctag ctgaattgct
    29521 atgacatggg attcgccgct aaactcatta aaggccttta gcataccgtc atagtcggtc
    29581 ctaatcccca tgggccaatg aggatcatct gttaaaagag attgacttat gtccccatgg
    29641 ggtactattc ccctctcgtc catcatcatg gaaacaagaa aatgcttgcc gccgacctct
    29701 ccttccgcca ccctatagtt gtcacaattg cccagaaccg caactccaac accagcctcc
    29761 cttagggcca tgcccagggc gccaaccttt accctatatg gtctgttttc attgtttcta
    29821 tgcagctttg ctatggacgg gttggctatc gccccttcat ccatcctata tcctgtgatc
    29881 tgatgcatta aattctcgat cttctccccc ctatatgggt atccatagga tgctgccacc
    29941 tgggaacccg ccaacccggt aacccgggct ccggaaccta gggttaaata ggcattattt
    30001 tgggaaaggc tcccaccggt attggtggtc atcattccgg cagccccaat gcccattagt
    30061 ctgtccaagt ttggggtgtc ggcatcatgg atatcctgcc agctgacacg gtctaaaaga
    30121 atgaggatga ctcttcccct gtcctctatt ttatggtccc tttgtcttaa ggcttccggc
    30181 ctgtgaccgc acaggataga attgttgggg atagccaata tagggagcat caacagaaaa
    30241 caaaatgtta tctttaggat gggataagta tggattcttt tcaatgtgga cctccgctta
    30301 aaatagatat ttatgtcttt tgtctaacat tattccccac ttccccctcc gcaacccaaa
    30361 aatatgccaa aatgaaaggg atgtttattt taaatataca aaacacaagg gacgattcct
    30421 taaaataagc aaagttaagg aaattattct ttaaaatagg caaaactaaa ggacggctct
    30481 ttttgccacc aagcaaaaag caccgtcccc tacgctttgt ctagatttga acgccatact
    30541 gacatttcat aatttcgtta tagatacccg cgactttagt catgggttat tgactcccct
    30601 ttgttgatta ggagtatccc cagggatact aaaattgcag ctgcgataat atttgctccc
    30661 aagaacctct cctttaggat tatataggat aacagagtgc caaacagggg tatggtaaac
    30721 ttatatatac tgaccctgcc gacgccattg tgcctaagaa ggatggacca tagggtaaag
    30781 gccgccgcgg atataaaccc taggtataat aaaagaagga tacccatggg cgtaaatgta
    30841 atgcttcttc ccccacccag ccatccgaag gatatcaata acagggaacc gaaaaacaac
    30901 tggtatccgg atatggcaaa gggagatata tccttggata tcttttttgt atagaacccc
    30961 gcaaaggcgc ctacaaggct tgagattatt ataaagcctt cgcctgtcag tgtaaaatcc
    31021 gtcccaaggc cggttcctgt catgttggcc gtgatgacgc ctataaagcc taggacagcc
    31081 ccggcaatcc tcttatgggt cagcttgtcc tgggagaaga aaaagtgcgc caacaaaacg
    31141 gtaaaaaaag tgctgctggc cgatagtatg gaacctaagg tgccgctggt atttgaaagt
    31201 cctatgtaga aaaacacata ttggatgctg gtctgcataa cccccaggga cacgacctta
    31261 cggacatggg ccctcttcag cttcaaggac ctgcccgtaa ccaaacaaaa cccaaatatc
    31321 atcatagagg acaggaaaaa cctatacccc gcaaagacaa tcttctcata ggtagcgtcg
    31381 ggccctatgg aaaacagctt atagcccgat ttaaccgatg gaaatgcgct cccccatagg
    31441 aaacagctta ttaaggccaa agccataagt acgtacgaat ttgtaaatat tgatccttct
    31501 ttttctttca attgaatccc cccgatattg aggtatatat acccaacccc aatggatata
    31561 taactaaatt ttgcttattc tatcataccc gttttcgggg tgtctaattg gccttgggct
    31621 attcccaaag aagatcggtc caccttttta cgcagatatc gtccccgcgg cggaagaagg
    31681 tggtaaaatt cgtttcctcc ctgcctatga gttcccgggt tatcagcgca ttacctgagc
    31741 tgtcttgttt atgggtttct actattatat attcggtttg tttgtttggg gctaatctat
    31801 ccggtacttg gcctggggaa tattctctat gggataaaac agatccgttt tcatccatct
    31861 ccaaaatagc aattttcaca ggagaaaaca tcgtctctag ggaaatcttg accgaagtac
    31921 tgtgcttttt gctctcaccg ttttcggtga tggttgtggt ttcctctatg gtctgtgaga
    31981 aagctccacc ctcattttcg gccccatcca tggtcatccc ggaaccgctt acggcataga
    32041 ccctaccgtc tgcgccctgg tagaccggat taatatagcg gcttatcatg cccggagtgg
    32101 cggcaatata gatgatcccc tctagggttg ttttctcctc gtcgtctcca ataaaaatac
    32161 cggtatgtcc gtcgctgatt gtctcatcag agccggtggt agtatagctt gtattaccgt
    32221 cctcttccgt aatagtcgcc gcaaagtatg atgccccgtt cacaccttca aatacatatt
    32281 cctttgtggt gcttgtttca ccggtttctt cgtcaactaa ctccagctcc tttaaaaccg
    32341 cgtataatcg gccttcatac cttcgggatt gatcttcagt tattatcccg ccatcggaaa
    32401 tcccgccaaa gttgtcatta aggtatccct caaagtcaaa caagtcgagg tattcgtctg
    32461 ttacaaaaac gcctatgagc ctgtcccccg tcggctcttc cccggcgtct gcccgggcaa
    32521 gttggcatcc ggccagagcg aatatgcaca tcgcaacgaa cagcatccca ataaacccct
    32581 tatgcttcat catcctcatc ctccaaatta ccgtcaaatt ttaagatatc gcccacatca
    32641 catttaagat gccagcagat cttgtttatg gtattaaacc gaaccccttt ggccttaccg
    32701 ctgcggagta tggaaagatt ggcttcggta attcccacaa gggcgctcaa ttccttagag
    32761 gtcatccccc ggtcctttag aacttcctca agatgaacct ttatcgccat ttcttcacct
    32821 catataatac tgtcgctgtc ttctttaagc actttatttt ctgcggtaat tcgagaaaat
    32881 aacaacaccg ccaatacaaa aacgattgaa aacaatggaa tttgcacctg gctattgatg
    32941 ttatgaagga attttgaaaa aatcaattgc aggatattaa acgctattgt gccgagcacc
    33001 gtaattacca gagaaaccct gcagattatt gagaggtttt ccgcttcaga aaccgaagct
    33061 acggagtatc ggttttctgc cagttcccca attaaggcga tccctctgtt gactattaag
    33121 atattcagca taaggggtat attatctacc aaaaactgca gaaccaagaa cacatagtcc
    33181 atgcctaaaa ggcgctcgtt ccccagattg cccaccctta aggctttaaa ggaatttatc
    33241 aaattgtttg cgcccggacc aaatataata aaaacaaata atgcattaac gagaaccagt
    33301 aaccctatca tatacttatg cagctttttt gtatcgctga aaaagaataa cctcagcgcg
    33361 cgcagtatag catagccgcc tataacggaa tagacggtga ctccaaacac gcctttcgca
    33421 aataaaatgc cgccgttgct gccagagtcc tgattaatca agccgggatt gataagaaga
    33481 tacatgataa caaagagtaa ggccgagaag accaagagca gaatatcctc tttataaaag
    33541 tcccttttcc ttttatgtat aagaagcgca gcgcagggcg caatacagaa tgcaatataa
    33601 aaaaggattg ccgctacgtt gcctacttga ccggataggg ataggattcg cagccccgat
    33661 ccaagctgcg caaaaggaaa tgacactacg gcgggaaaaa gctcatgggt taaggagccc
    33721 atagcagcga atatcaagca taccgcagct tctgtagcca agataaggga atatattctc
    33781 cggttcattt caacaccccc attattgttt tacgat
//
LOCUS       DASRNX010000066         5608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_597505,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000066 DASRNX010000000
VERSION     DASRNX010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597505"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            642..2249
                     /gene="cimA"
                     /locus_tag="VFD89_02655"
     CDS             642..2249
                     /gene="cimA"
                     /locus_tag="VFD89_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HZJ57120.1"
                     /translation="MVGNLIKNKVFIYDTTLRDGAQAEGISFTVKDKLRIARRLDSLG
                     VDYIEAGNPGSNPKDMEFFDRIKKRPLSHAKLAAFGSTRRPGIKAEDDVNVRALLTAD
                     TEVVAIFGKSWDFHVTDIIRTTLEENLNMIRDTVSFLKTEGKEVVFDAEHFFDGYKAN
                     REYALKTLEAAEGAGADSLALCDTNGGCFPSEITEITKDVVERLNLSIGIHCHNDGGM
                     ADANSIVAVEAGANQVQGTFNGYGERCGNANLSVIIPNLQLKRGKVCIKEEGMKELSA
                     ACRYISEIANLPFNERQPYVGRSAFAHKGGMHIDGVQKNQHSFEHIVPELVGNERRIL
                     MSEVSGRSTILRGIQRVVPQVSKESEETILIMDKLKQLEHEGYQFEGAESSFELMVRR
                     VLGKSKIFFDVKDFRVLCEEHWQNDYSASAIIKLKVDGNEEIIAAEGDGPVNALDTAL
                     RKALEIFYPQLKRMRLTDYKVRVVDTTKGTAAKVRVHIESTDGKRVWGTVGLSSNIIE
                     ASWNALIDSIEYFLYLNGNDENETKNI"
     gene            2264..3526
                     /gene="leuC"
                     /locus_tag="VFD89_02660"
     CDS             2264..3526
                     /gene="leuC"
                     /locus_tag="VFD89_02660"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZJ57121.1"
                     /translation="MGMTMTQKILAAHAGLDKVVAGQLIMADLDLVLGNDVTSPVAIN
                     EFNKIGAEGVFDKTKIALVPDHFTPNKDIKSAEQCKLIREFAFDKGIENYFEIGEMGI
                     EHALIPEKGLVVGGDVVIGADSHTCTYGALGAFSTGVGSTDMAAGMANGKAWFKVPEA
                     IKIILKGNLPEWVSGKDVILHIIGMIGVDGALYKSMEYTGPGVSSLSMDDRFTIANMA
                     IEAGAKNGIFEIDEKTIAYMKEHSTKEYTTYKADEDAKYVKTIEIDLSTLRPTVAFPH
                     LPDNTRTIDEVGDVPIHQVVIGSCTNGRIGDLRIAAEILKGRKVKKGIRTIIFPATQK
                     IYLKAMEEGLLRIFVEAGAAVSTPTCGPCLGGHMGILASGERAVSTTNRNFVGRMGHV
                     ESEVYLASPAVAAASAITGKISSPEEVM"
     gene            3529..4014
                     /gene="leuD"
                     /locus_tag="VFD89_02665"
     CDS             3529..4014
                     /gene="leuD"
                     /locus_tag="VFD89_02665"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZJ57122.1"
                     /translation="MKGKVIKYGDNVDTDVIIPARYLNTSDIEELAKHCMEDLDKDFV
                     NKVEPGDIMVAGKNFGSGSSREHAPAVIKASGISCVIAETFARIFFRNAINIGLPIME
                     CKEASEDIEDGDVVLIDVYSGEITNETKGKSYKGTPFPGFIQNIIKAEGLVNYIKEEG
                     K"
     gene            4017..5081
                     /gene="leuB"
                     /locus_tag="VFD89_02670"
     CDS             4017..5081
                     /gene="leuB"
                     /locus_tag="VFD89_02670"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZJ57123.1"
                     /translation="MKIALIPGDGIGPDIIDQAVKVLDAISRKYDVKFSFEEVLMGGV
                     AIDKTGVPLPEETMDICKNSDAVLLGAVGGDEWDNLPGHLRPEAGLLGLRKGLGAFAN
                     LRPAILFPQLKNASTLKPEVLGDGLDIMVIRELTGGLYFGERGRERKGTEERAWDTLP
                     YTYSEIERISRVGFELAMKRGKKLMSVDKANILESSRLWREVITEIAKDYEEVTLSHM
                     YVDNAAMQLIRNPKQFDVIVTENLFGDILSDEASMLTGSLGMLPSASLGEGTQGIYEP
                     IHGSAPQIAGQDKANPIATIMSVAMMLRYSFNMEEGALAIERAVSKVLDKGYRTGDIM
                     EDGKELVGTDKMGDLIVAEI"
     gene            5136..5450
                     /locus_tag="VFD89_02675"
     CDS             5136..5450
                     /locus_tag="VFD89_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZJ57124.1"
                     /translation="MNKKIYNKLIRDNIPDIIEASGKRCTVETVEGEELVKLLNEKLQ
                     EEVDEYIASNDIEELADIVEVIRGILHNRGVTVEELEGIRFKKKEERGGFSGGIKLLE
                     VY"
BASE COUNT         1700 a         1013 c         1480 g         1415 t
ORIGIN      
        1 cctttaaaaa gattgtttcc aattgacaca catactctct cctttcattt taatttccgg
       61 tgttagtttg ctcttttaaa ccggattaat acatgataga actatcttaa taatctcatt
      121 tcatgcacag tgaatacagg ctgtgtgaaa aggcaccttt tttacttttt gtcattgcta
      181 tgaaaggtag gggtggacga ccctgtccac ccgcgggcag acagagtcgt ctgcccctac
      241 aaaaacaatg ttttcattta tcgtggtgtg gctagtacat gtaaattgct atgaaatgta
      301 ggggtggacg accctgtcca cccgcgggca gacagagtcg tctgccccta caaaaaacaa
      361 tgttttcatt tatcgtggtg tggctagtac atgtaaattc tgagtgtagc gaagaatctc
      421 gtttttagac cacttgggct ccttcacttc gctcgggatg acaattttcg cacagtctga
      481 atagcctgta gtattatctt tcaatatcct gttaatatga tagtcccgac ttttttcccg
      541 ttttcagtat ttttatcttg caataaataa aataatgggt taaagtaagg gctatataaa
      601 tgtaacagat aaaacgttat ataaaataaa gggggagtga tttggttggg aatttaatta
      661 agaataaggt gtttatttac gatacgactt tgagggacgg tgcccaagcc gagggcatat
      721 cctttacggt gaaagataag ctgaggatag ccagaaggct ggactcccta ggggtggatt
      781 atatagaagc ggggaatccg ggatccaatc caaaggacat ggagttcttt gacaggataa
      841 agaagaggcc tctgagccat gcgaaattgg cggcctttgg cagcacccgg agaccaggaa
      901 taaaggcgga ggacgatgtc aatgtgaggg ccctccttac ggccgatacc gaggttgtag
      961 ctatattcgg gaagagctgg gattttcacg ttacggatat tataaggacc actttggagg
     1021 aaaacctcaa tatgattcgg gatacagtat cctttttaaa gactgagggt aaagaggtag
     1081 tttttgatgc ggagcatttt ttcgacggct acaaggccaa cagggaatac gctctgaaga
     1141 ccttggaagc ggctgaaggg gcgggagccg attccctagc tctttgtgat accaacggag
     1201 gttgttttcc ttctgaaata acggagataa ctaaggatgt ggtggagaga ctcaatctat
     1261 ccataggcat ccattgtcat aatgacggag gtatggcgga tgcgaattcc atcgtagccg
     1321 tggaggcagg ggcgaaccaa gtacagggaa cctttaacgg ctacggagag cgctgcggca
     1381 acgccaatct gtccgtaatc atacccaatc ttcaattaaa gcggggaaag gtctgtataa
     1441 aggaagaagg gatgaaagaa ttaagtgcgg cttgtcgtta tataagcgag atagcaaatc
     1501 tgcccttcaa tgaaaggcag ccctatgtgg gcaggagcgc ctttgcccat aagggcggga
     1561 tgcatataga cggggtccaa aagaatcaac attcctttga acatattgta ccggaacttg
     1621 tggggaacga gcggagaata cttatgtccg aagtttcagg caggagcaca atattaaggg
     1681 ggattcagag agttgtgcca caggtttcca aagaatcgga agaaaccatc ttaattatgg
     1741 acaaattaaa acaattggag catgagggct accaattcga gggtgcggag agctcttttg
     1801 agctgatggt aagacgggtt ctcggaaaat ctaaaatctt cttcgacgta aaggacttca
     1861 gggttttatg cgaggagcat tggcaaaatg attacagtgc gtcggctatc ataaagctga
     1921 aggtagacgg aaacgaagag atcattgcgg cggaagggga tggtcctgta aatgccttgg
     1981 acacagccct tcgtaaggcg ctggagatat tttatccgca gctgaaaagg atgcgtttaa
     2041 cggattataa agtaagggta gtggatacta caaaaggcac ggctgcaaag gtaagggttc
     2101 acatcgaatc caccgacgga aaaagggttt ggggcacggt aggtctttcc agtaatatca
     2161 ttgaagccag ctggaatgcc ttgatagatt ctatagaata ttttttatac ttgaacggca
     2221 acgatgaaaa tgaaactaaa aacatatgag gggagtgaag gttatgggaa tgaccatgac
     2281 acagaagatt ttagcagccc atgccggtct tgacaaggtt gtggcggggc agcttataat
     2341 ggcagatttg gatttagttt tgggcaacga tgtaaccagt cccgtagcta taaatgaatt
     2401 taacaagata ggagcagaag gggtctttga caaaacaaag atagcccttg taccggacca
     2461 ttttactccc aataaggata taaaatctgc ggagcaatgt aagttaataa gagaatttgc
     2521 ttttgataaa gggattgaaa actactttga gataggcgag atgggtattg agcatgcctt
     2581 aattccggag aaaggcttgg tagtcggcgg cgatgtggta ataggtgccg attcccatac
     2641 ctgcacctac ggggctttag gggccttttc caccggggta ggtagtacgg acatggcagc
     2701 cggaatggcc aacggcaaag cttggtttaa agtgcctgag gccatcaaaa taatcctgaa
     2761 agggaatctc cctgaatggg tcagcggaaa ggatgttata ctgcacatta taggcatgat
     2821 aggggtggac ggcgctctct ataaatccat ggagtatacg ggacccggag tttcatctct
     2881 atccatggac gacagattta ccatagccaa tatggccatc gaagccgggg caaaaaatgg
     2941 gatatttgaa atagatgaaa aaaccattgc ttatatgaag gaacattcca ctaaagaata
     3001 caccacctat aaggctgatg aggatgcgaa gtatgtaaaa accatcgaaa tagatttatc
     3061 tactttaagg cctacggtag cttttcctca tcttcctgat aataccagaa ctatagatga
     3121 ggtaggggat gtgcctatcc atcaggtggt aataggttct tgcaccaacg gaaggattgg
     3181 ggatctgaga atcgctgctg aaattttaaa gggcaggaag gtaaagaagg gtatcagaac
     3241 catcatcttt cccgctactc aaaagattta ccttaaggct atggaggaag gtcttttaag
     3301 gatatttgtc gaggccggtg ctgctgtcag tactcctacc tgcgggcctt gtttgggcgg
     3361 tcatatgggg atacttgcat cgggagaaag ggctgtttct acaaccaaca gaaattttgt
     3421 aggcagaatg gggcacgttg agagtgaagt gtatttggca agtcctgccg ttgcagcagc
     3481 ttcagccata acgggcaaaa tttcgtcccc tgaggaggtt atgtagacat gaagggtaaa
     3541 gttataaaat atggagataa tgtggatact gatgtcatca ttcccgccag atatctgaac
     3601 accagtgata tagaagaact ggcaaagcat tgcatggaag atttagacaa ggattttgta
     3661 aacaaggtag agcccggaga tataatggta gcgggcaaaa actttggcag cggttcttcc
     3721 agagagcatg ctccggcggt aatcaaagcc tccgggataa gctgtgtcat cgcagaaact
     3781 tttgccagaa ttttttttag aaatgctata aatatcgggc ttcctattat ggagtgtaaa
     3841 gaagcctctg aagatataga agatggggat gttgttttaa tagatgttta cagtggagaa
     3901 ataactaatg agactaaggg aaaaagctac aaaggaacgc ctttcccggg atttattcag
     3961 aatataataa aggcggaagg ccttgtaaac tatataaaag aagaggggaa atagacatga
     4021 agatagcctt gattcccgga gatggaatcg gacctgatat aatagaccaa gccgtgaagg
     4081 ttctggatgc catttcccga aagtatgacg ttaaattcag ttttgaagag gttttgatgg
     4141 gcggggtagc catagataaa accggggtac cccttcctga agagaccatg gatatatgca
     4201 agaacagcga tgctgtgtta ctgggtgccg tgggcggcga tgaatgggat aacctgccgg
     4261 gccatttgag gccggaagcc ggacttttag gacttcgaaa gggcttagga gcctttgcca
     4321 atcttcgtcc cgccatattg tttccccagc taaagaatgc ttccaccttg aagccggaag
     4381 ttttggggga tggccttgat attatggtta tccgcgaact gacgggaggc ctttactttg
     4441 gagaaagggg cagagaaaga aaaggcaccg aggaaagggc atgggatact ttgccctata
     4501 cctattctga aatcgagagg atatcgagag tgggtttcga actggccatg aaacggggaa
     4561 aaaagcttat gtccgtagat aaggccaata ttctagagag ctctaggcta tggcgtgagg
     4621 ttatcaccga aatcgccaag gattacgaag aagtcaccct aagccatatg tacgttgata
     4681 atgcagctat gcagttaatc agaaatccaa aacaattcga tgtaatagtt acggaaaatc
     4741 tatttgggga tatcctgagt gatgaagcct ccatgttaac aggttccttg ggtatgttgc
     4801 cgtctgccag tttaggcgag ggaacccaag gaatttatga gcctatacac ggctcggcac
     4861 cgcaaattgc aggacaggac aaagccaacc ccatagccac tatcatgagt gttgccatga
     4921 tgttaaggta tagttttaat atggaggaag gggccttggc tatcgaaaga gctgtttcca
     4981 aagttttaga caaaggctac agaacaggag atattatgga agacggcaaa gagcttgttg
     5041 gaactgataa gatgggggat ttgatagttg ctgaaattta aaaaatgcac cggggacggt
     5101 tcctgttgca ttcaacctca aagtaggtga tataaatgaa caagaaaatc tataacaaat
     5161 tgataaggga caatatcccc gatattatag aagctagtgg taaaagatgt acagttgaaa
     5221 ccgtggaagg ggaagagttg gtaaaattat taaacgaaaa attgcaggaa gaagtcgacg
     5281 aatatattgc gagcaacgac atagaagaac ttgccgatat agtagaagtc atccgcggta
     5341 ttttacacaa cagaggagta accgttgaag aattagaagg cataagattt aagaaaaagg
     5401 aagaacgggg cggattttcc ggggggatta aattgttgga agtgtattag gattaacacg
     5461 gggacggcag actgtgtgaa aaagcacttt tcttacttat tgtcatcctg agacgtacag
     5521 aataaaccct cccgcgaagg atccccaaag gatgagatcc ttcgtgagca ccgtttatac
     5581 tgtccgtctc aggacgactt catcggat
//
LOCUS       DASRNX010000067        20543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_13389,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000067 DASRNX010000000
VERSION     DASRNX010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 20543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20543
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13389"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..681)
                     /locus_tag="VFD89_02680"
     CDS             complement(187..681)
                     /locus_tag="VFD89_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016818390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZJ57125.1"
                     /translation="MLSSPPKVCDVLTKNSYWLVTGLFNLDDCIAFMTCQSGKIFADV
                     LERELAPHKATRSQWIAMHYIYNYRNLTQRELADKMSVSQPTVVRFLQILESKGYLKR
                     SGANEDKRVKQLELTEAGVKVYLDILPAVKKFKTDTVAGISQKDLDILKNTLDTMIKN
                     TLGE"
     gene            complement(722..1162)
                     /locus_tag="VFD89_02685"
     CDS             complement(722..1162)
                     /locus_tag="VFD89_02685"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016818391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZJ57126.1"
                     /translation="MLTTFKATAKNLPDGMQVETQARGFKIIMDEPKDLGGTDIAMNP
                     VEALLCALGACQAIVARAFAAAHNFTFEEFHVELEGDLDPDGFLGKADVRNGFQEIRF
                     KMHFKTDESQEKAEAFAEFIEKTCPVGDCLENGVKLVPAGVVRY"
     gene            complement(1501..2571)
                     /locus_tag="VFD89_02690"
     CDS             complement(1501..2571)
                     /locus_tag="VFD89_02690"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HZJ57127.1"
                     /translation="MAEKVDLRDKFLGCIAGVHIGSSMGATVEGWPYQRIEETYGTVD
                     KLRPYEHYNNGWIREAGTTEDGVERQKLIITAIIEKKNRVNAEDVRKIWLRDIKPESA
                     GMVSEPFEAILLAMAKTGIPARDLGRYCDYAGLNSFARACHPIGLINAGDVLGAADDV
                     LEVGQLYQTSNSRGLKWAMVTGVAIAEATKPNATVDSVIGAVFDICDPDLVVKELDKQ
                     LKNTAHCKDFRELREAFDSEYSGHGMTYAFAFANEVVTKGLCIFRMVKGNTKDAIIAG
                     VNLGRDTDCLAAVAAGISGSLTGSSSIPQEWMDQVDSATKLNIYTNSQRTLQEHADGL
                     YKAYIARYRKMNALAKKMGISI"
     gene            complement(2779..3681)
                     /locus_tag="VFD89_02695"
     CDS             complement(2779..3681)
                     /locus_tag="VFD89_02695"
                     /inference="COORDINATES: protein motif:HMM:NF037850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HZJ57128.1"
                     /translation="MISGTESFPIEAYENWEAVKKYPHVIGDFVWTAWDYLGESGIGH
                     TSYEGEARGLKKFPWHVANCGDLDICGRKRPQSHYRDFLWNDRNKPYIGVWHPKDWGK
                     EEKISPWGWPDVLESWSFPGYDGSKVKVVVYAAGDQVELYLNGELLDKKSCGAKTRYI
                     QEFEIQYIPGKLEAVAYADGLEVSRASLCTVGRPQKIIFRSENHRDNEDNMAFIEVEI
                     ADEHNRLVPYAEDEIEINVRGGKLLAMGSGDPKAVQDYTEAIGSAWRGRAMLVVQRDD
                     DSELVEIRAMGKGLDDGVMTISYR"
     gene            complement(3641..4411)
                     /locus_tag="VFD89_02700"
     CDS             complement(3641..4411)
                     /locus_tag="VFD89_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57129.1"
                     /translation="MGGVFIKILDFSKDWYFAYAENEDWVNQYGGHANEPVDLPHDFK
                     IGLKRCDDPFSGPSEGFYPGGIGYYKKVFNIAGDMLSQRALVYFDGVYRMCEVRLNRD
                     LIVTHKGGYTGFFADLTGRLREGENILQVKVNASLPNASRWYTGAGIYRKVTMLTGSI
                     PSIEPWGIHVETLTDTLGTASADIKIKTKIYNPEKRACNIVHKIFDRDAALIATKEDI
                     GVKDKSAVTVKVPDPKPWSADFPRAGDFRYRIISYRGL"
     gene            4732..7233
                     /locus_tag="VFD89_02705"
     CDS             4732..7233
                     /locus_tag="VFD89_02705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZJ57130.1"
                     /translation="MELTFCGGAGEVGASCLLLNIDGKNLLLDSGIRMGDAKDSLPDF
                     RIIQEKGGLDAILISHAHLDHTGSLPIISREYPNSVIYMTHATKDLVRVLLYDSLKIM
                     GYSEGEIPIYAESHVQDMLNRIRCFSPEYTIDLFEDSKVRATFYHAGHIAGAGSIYIQ
                     GEEGSFFYSGDFSMANQHTVGGAAIPTLRPDVAVFESTYGDKLHANRQIEEERLVATV
                     QKVIERQGKILIPAFALGRAQEVILILKRAMNRGRLPKFNVYVDGMVREICRMYRLNP
                     NYLRPSLAKRVWRDGEIFFDDWIQSVDDREMRDKIVSSDEPCCIISSSGMLTGGPSQW
                     YAEKIISDSKNFIAITGYQDEEAPGRQLLNLLDDTPEDAFLSIGDRKLPIKCELGKYN
                     LSAHADRGEIIGLAHNISPKEIFLVHGEGQAMGELGRHMQGDVRGRVYIAANGESYDI
                     NIGKARRQRRFASIPTLGKEGLPQGDDFITLWEHIIDNRGDRSSYTSEELIRIWADKS
                     SSEEDTRDVMNAISQSLYFEQDYRRPFLFRPVSQDSINEQLQEMEGPMEVNQMLGLVD
                     EYFPPQTGLYRRGARFDEKIVLLYFKFPLIAQREFKDKFQEFESITGWKVQINEEYNL
                     EAAEDLLHTLMPTQIDWSGKLSYYRLEGVFKTAIEGDIDLGKVCEDFEARTGLPLIIE
                     DGKGSLPLEPVSATHGQMEQNKAFQIIDEAFAGKPVRVYKKGLKRDGDTPYIELAFIT
                     PEIGEKWQHLIEELETQTWWNIRINPSPNQNEVLRLARRLLEGEGLLLRRNPSFIQID
                     QAVRVTPAEPWDPEVLEEIKDVFAGETGYTLVVSK"
     gene            complement(7293..7958)
                     /locus_tag="VFD89_02710"
     CDS             complement(7293..7958)
                     /locus_tag="VFD89_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HZJ57131.1"
                     /translation="MKSELTELVLILDKSGSMSGLESDTIGGYNSMLQKQREVEGECR
                     ITTVLFDNNYELLHDRIDIRAVSPITEKEYFVGGTTALLDAIGRTVHKIISVQKSTAK
                     EYQAEKVIFVIITDGMENASREYSAGKVKALIERQEEKYGWEFIFLGANIDAVETAGH
                     FGIRANRAQNFHADSAGVGLTFSVMSDVVANYRESNSVSEDWKDEIEKDYKGRGGKNP
                     ESR"
     gene            8294..8869
                     /locus_tag="VFD89_02715"
     CDS             8294..8869
                     /locus_tag="VFD89_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZJ57132.1"
                     /translation="MGAEEAKIIKLCKGNKREGYNLLYEKYEAYIYSLCFRYTRSKED
                     SLDLLQDVYLKIYQGMPRFKEGRNLLPWIRKITINTCLNFVTRRRDNALSLSMQIDEN
                     ENTLEDIIPSHTSVVDEFLSIRAKKILKSAILGLPKDMKMAIILRHMEAMSYREIAKA
                     MSIPIGTVKTLLFRGRKILKGQLMAQGVWEE"
     gene            8871..9305
                     /locus_tag="VFD89_02720"
     CDS             8871..9305
                     /locus_tag="VFD89_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57133.1"
                     /translation="MICCLDKDILQEKLDGSLGHVEDMLLSEHLRCCKSCSREYSQMS
                     SVFDGIDAIKGPIDIPGGLFLIRNQVLNRIPELESSSTYSLGEFIRIQKTAVKSSGKF
                     LEYVPGTKYVEAGIKKAPKIFYRAFSFAMIRGIKFATGRSRT"
     gene            9410..9964
                     /locus_tag="VFD89_02725"
     CDS             9410..9964
                     /locus_tag="VFD89_02725"
                     /inference="COORDINATES: protein motif:HMM:NF022615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HZJ57134.1"
                     /translation="MVIIKAQIFGPLKWICLTFKMRDMTEKKLGVRFLGIPIKIKDNK
                     KDAPIGSKVKRNNKGKKTRKKGRKPAEGIPAGIVNLIVRALRHIKPRRLEVKGRYGFN
                     DPYHTGIACSLINTIIPHKDKYNVDLIPVFDEEVIEGKFLIEGYIVPVYLFWLFIKVF
                     VLSRLKKAFNKSFRGRFKRSVPAQ"
     gene            9992..10375
                     /locus_tag="VFD89_02730"
     CDS             9992..10375
                     /locus_tag="VFD89_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HZJ57135.1"
                     /translation="MSVNVNVGENIDLLFQNLEDFIKNKTIFGEPMQIGDATLIPVVD
                     VSFGLGSGGGDGSDNKGNGGVGGGAGIGAKASPTAIIVMKDNNVEVLPIRKSSGLEKL
                     LEMVPDLVDRVRAEMPTKNKDEDKE"
     gene            complement(10535..10990)
                     /locus_tag="VFD89_02735"
     CDS             complement(10535..10990)
                     /locus_tag="VFD89_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HZJ57136.1"
                     /translation="MGSLGDLVRIREEIRKKEEGHEKEGKVRIIVGMGTCGIMAGARK
                     TMKAFLADTINRNLDVEVTQAGCIGLCELEPIVEVILPGQPSVIYGKVSGDRAREIVY
                     RHLVNGSVIEEWVIGKRQIVWDRERAGGSFRRGTIPSTTNTIHNRRMIW"
     gene            11440..12207
                     /locus_tag="VFD89_02740"
     CDS             11440..12207
                     /locus_tag="VFD89_02740"
                     /inference="COORDINATES: protein motif:HMM:NF017655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HZJ57137.1"
                     /translation="MDSPHTTYARPGEMMSGTFLKIIAIMAMLTDHIAWAFVPTHSLL
                     GQLMHVVGRLTAPIMCFFIAEGYHHTRNVKRYALRLGLFALISHAPYIYFESGRPPIY
                     YENGIKIIYGTSVIYTLLLGLILLMIWNDERIKERVKHLLAILIVVASAPGDWSFFAV
                     LWIWAFGVHHGNIKKQLQSFSLIAILPVTFSIILFSQGVWWGQLFQLGVYLAIPFIYL
                     YNGKLGYKKGWVWIKWVFYAFYPLHLLVLGLLKYIGR"
     gene            complement(12392..12931)
                     /locus_tag="VFD89_02745"
     CDS             complement(12392..12931)
                     /locus_tag="VFD89_02745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZJ57138.1"
                     /translation="MAGCAQKDVKYPTVTMKIKGRGDIVIELYPEKAPETVANFVSLV
                     EDGFYDGLTFHRIIPGFMIQGGDPEGTGTGGPGYSIKGEFADNGFEDNDLKHLRGVIS
                     MARSQHPDSAGSQFFIMHDDSPHLDGAYAAFGKAIKGMDVVDAIAKTPTDMRDYPTEA
                     QVIKKATVQLPPDYEKEDE"
     gene            13168..14829
                     /locus_tag="VFD89_02750"
     CDS             13168..14829
                     /locus_tag="VFD89_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZJ57139.1"
                     /translation="MIVGLDMGGTHIDGVIIENGTIINTVKKPRADGDIFGSIWGALE
                     GLLLDQDTTKIERINLSTTVSTNAIVEDKTTPVGMVIQTGPGLSGDFLACGSENVFIS
                     GCIDHRGKVVERLDSKEIDRAKELFIREGLSACAVVTKFSTRNFEHEVQIKRLLGDGF
                     DHITMGHTLSGMLNFPRRVFSAYLNAAIHNTFNRFSHSIKKAMIEREINAPLFILKAD
                     GGTTVLAEAENRPIETILSGPAASFMGLNALLKTQEDALLLDIGGTTTDIFFLADGVP
                     LFEPMGIEINSYKTLTRAIYSVSMGLGGDSSVRVENREIMIGPRREGLPYALGGPCPT
                     PTDAMIALGLVEFGDRKKATAAMKSLGQILGLSVEETARDILDIMADMIKSKVDELLD
                     KINDKPVYTVHELLHGRKLRPRLINLIGGPSKYLSPVIEKAFGLPCHYPKDYAIANAI
                     GAAMAKMTQDITLVADTERRLLTVGEFGVRERIGRGFTLKDARKRAIGLLEDRAVAQG
                     ANKGEIETEIIEENSFNMVDGFYTVGKNIRVKAQIKPGLAGDLKL"
     gene            14871..16181
                     /locus_tag="VFD89_02755"
     CDS             14871..16181
                     /locus_tag="VFD89_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZJ57140.1"
                     /translation="MIKAKNSLGLVFFPAFDWAISPTHPEREERLLYTRDQLFEEGIS
                     DIEGIHFYNPIIAHERDIQRVHFCVPDVASRLTDSHLIAAGGAIKAMDAVMKGDVDKA
                     FAMVRPPGHHAQRVVYGDRGFCVINVEAVMLERLRQDYGDLKVAIIDTDCHHGDGTQD
                     IYWNDKNTLFISFHQDGRTLYPGSGFLGEFGGPAAYGYNINIPLPTGTGDEGILYVLE
                     NVVLPILEDYKPDIIINSAGQDNHYTDPLTNMNFTAGGYARLNSKLNPDIAVLEGGYS
                     IEGALPYVNLGIILAMAGLDYSGVREPDYDEEALKQTEGTTEYIKELSHLVYSRWQNR
                     ERLWAEDIKGKDIVELERQVYYDTDGILENQTQTFISCRKCSGVNTIDSSSDGGYHIY
                     AITIPRDACAECIHKGHKIYKEASPGDYTNIYLQDRVNDTCFSK"
     gene            complement(16187..17053)
                     /locus_tag="VFD89_02760"
     CDS             complement(16187..17053)
                     /locus_tag="VFD89_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZJ57141.1"
                     /translation="MGIKDNDKFSSIVTSDPASWGLGTSSSLFSSLDEAVFKECMDAG
                     IENMEVCLFGDWTQEPDEKVSKACADIKRKADNAGINIWSIHLPFGREWDISEPDSDK
                     RKSAIDGQVRLLELAAVMEPKKAVIHGSFEPISDNERDARISACRDSLWAIGEAAATL
                     GIELAIECLPRTCLGNSGEEMAELIRGNDKVGVCCDTNHMFKESPGNFIRRIGSRIVT
                     LHISDFDGIDERHWMPGEGIVDWNDVIAALTEVGYVGPFTFELSHKNTDETYTPEKIA
                     KCWEGLLRAYRA"
     gene            17211..17960
                     /locus_tag="VFD89_02765"
     CDS             17211..17960
                     /locus_tag="VFD89_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZJ57142.1"
                     /translation="MDLKIKEVADLVGISVRTLHHYDQIGLLTPTKRTESGYRLYSGS
                     DLEVLQQMLFFRELDFPLKKIKEIIESPSFDRREALLLHKKMLLEKRNRIDKILATVD
                     KTIRYMKGEIQMSNKEKFTGFDFGDNPYEKEARRRWGDDAVEKSNAKIYGMSGAEREA
                     MGDKMNAIYRRLAALRDTPPESDDAQTAIKEWHDFLNQNTGHHYSLDAFKGLGQMYVG
                     DERFTNNIDKFGESLAEFMCEAMAVYAEKNR"
     gene            18228..19370
                     /locus_tag="VFD89_02770"
     CDS             18228..19370
                     /locus_tag="VFD89_02770"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HZJ57143.1"
                     /translation="MDNSLRSIKYIDLSQDKAGQYPVDKEENQYLGHPDSILLDDGTI
                     LVFYPKGHGKGPIVMKKSADGGKTWGQRLPTPKSWADSQETPTVYQVIKPDGTKRLEL
                     ISGVPWNEGGFKTSYSEDDGQSWTEFEHHFPGLHTIVAHASLTRLKDDGGNWDNRWMG
                     LFHDHEFNNWMSILSFDDDGQGRWSSPQRLLEEHDEIEKHAQMCEIEVIRSPDGRQLA
                     LLARAQRKITNAIVAFSDDEGQTWSRPREVPWPLMGERHKAQYDPVSGRLLITFREIM
                     RKERNNGDEKWIAGDWCAWVGTYDDLYKGRMGEYYIRLIQDFTGDCGYAGNIALPDGT
                     FVLTSYGHWDKDYHNPYVMTVRLKLSQLDERFSHLKFNNPYHNPLY"
     gene            complement(19339..20304)
                     /locus_tag="VFD89_02775"
     CDS             complement(19339..20304)
                     /locus_tag="VFD89_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZJ57144.1"
                     /translation="MKLLTTFVPGKEDITKMEKLGACVTVFSSEEELVQSHKVEEADA
                     IIANHRTLQIDFLDKCKKLKWIHVPHVGIERLPMEYLKERNIVVTNARGTAGVPISEN
                     IMTMMLMLARKSLPSFKNQLAREWKSPGSVFNLYNKTVGLLGTGDVGSETAKRAKAFG
                     MKAIGYNTTGEPLEYFDKVYSADGLNEFLAQCDFIVCTLPPTPRTENLIDEEQFKCMK
                     PTVYIINISRAIIFNEEILYSYLKDKKIAGAALDVFMDEFRLGYLPKESPFWDLDNLI
                     ITPHMAGAGDQWNYFSTNIIMHNVKCFCEGRTNDMINIRDYDKGY"
BASE COUNT         5611 a         4778 c         4536 g         5618 t
ORIGIN      
        1 aactcccata agttattatt aatttcaatg tttcgcacta atacaagaag ccacggaacc
       61 tgattccctg gctttcttat gcttggttac atattataat accctacaca ccttacaaat
      121 tcaatgcctg attaggttaa agtagtatat agcaccataa ccaatggtct gttaacggta
      181 agctaattat tcccctaagg tattcttaat catggtatcc agggtatttt ttaaaatatc
      241 aagatccttt tggctgatac cggctacagt atcggttttg aattttttaa ccgcaggcag
      301 gatgtctaag tataccttaa cccctgcttc cgtcaattcc aattgtttca cccttttatc
      361 ctcattcgct ccggaacgct ttaaatagcc cttgctctct aggatctgaa ggaatcttac
      421 caccgtgggc tggcttacag acatcttatc ggccaattcc cgctgggtta agttcctata
      481 attatatata taatgcatag ctatccactg ggaccttgtg gccttatggg gtgcaagctc
      541 cctttccagt acatctgcaa aaatctttcc gctttgacat gtcataaacg caatacaatc
      601 atctaagtta aacaatccgg tcaccaacca atatgaattc tttgttaaaa cgtcacatac
      661 cttggggggg ctgcttagca accccccatt gtttacagtt attagtatct tttatttaca
      721 actagtatct taccactccg gccggtacca atttaacccc attttcaaga caatccccta
      781 cagggcaggt cttttcgatg aactccgcaa aggcctccgc cttttcttgg ctttcatcgg
      841 ttttaaaatg cattttaaaa cgaatttcct ggaatccgtt tcttacatca gccttaccta
      901 aaaatccatc gggatctaaa tccccctcca gctctacatg aaattcttca aatgtaaagt
      961 tatgggcggc agcaaaggct cttgctacga ttgcctggca tgcacctaat gcacacaaca
     1021 aagcttcaac ggggttcata gcgatatcgg ttcccccaag atccttgggc tcgtccataa
     1081 taatcttaaa tcctcttgcc tgcgtctcaa cctgcattcc atccggtaag ttcttggcag
     1141 ttgctttaaa agttgttaac attacagcat ctcctttcaa acatggtgtt ttttgttacg
     1201 ccttgttaat actttaacat gttgttttgc gcaagtcaat agttagctaa gcaattagaa
     1261 tggatattaa acgttgatcg gggataaata ttacaataaa ttattttata ataaaaatat
     1321 gatataaagg ccccatacat acaaaagata gaaaaacaag aagacatcca gaattgatct
     1381 aaaaagagca gtccatatgg actgcccttt ttgattccct ttatcctttt caaaaccatg
     1441 accatactga ttagataagg gcattctccg cctttgggct ggatagcgag gtttaattcc
     1501 tcaaattgat attcccatct tcttagctaa tgcattcatt tttctatatc tcgctatata
     1561 tgctttatat agaccatcag catgctcttg tagcgttctt tgtgaatttg tataaatatt
     1621 caattttgta gcactatcta cttgatccat ccactcttgt ggtatagaag atgaaccggt
     1681 aagcgaacca gagatacctg ctgcaactgc tgcaagacaa tctgtatccc ttcccaaatt
     1741 gacacctgct attattgcat cctttgtgtt tccttttacc attctaaaaa tacacagccc
     1801 ttttgttact acttcattag caaatgcaaa tgcataagtc atcccatgcc cactatattc
     1861 tgagtcaaag gcttctctca gttccctaaa atccttgcaa tgggctgtat tttttaactg
     1921 tttgtctagt tctttaacta ctagatcagg atcacaaata tcaaacactg ctccaattac
     1981 gctatctaca gtagcgttag gttttgtcgc ttcagctatg gccactccgg taaccatcgc
     2041 ccatttcaac cctctactgt tgcttgtttg atatagctgt ccaacttcca aaacatcatc
     2101 ggctgcacct aaaacatctc ctgcgttaat tagaccaata ggatggcatg ctcttgcgaa
     2161 actatttaat ccagcatagt cacaatacct tcctaaatcc cttgcaggta taccagtctt
     2221 agccatggca agtaggatag cttcaaaggg ttcagaaacc atacccgctg attctggctt
     2281 tatatcccta agccaaatct ttcgtacatc ttcggcattg actctgttct tcttctctat
     2341 tatggccgta ataatcagtt tctgtctttc cacaccatct tccgtagtgc cagcctctct
     2401 tatccatcca ttattatagt gctcgtaggg acggagttta tctacagttc catatgtctc
     2461 ttcaatcctt tggtatggcc atccctctac ggtagcaccc atagatgacc ctatatgtac
     2521 cccggctata catcccaaaa acttatccct tagatctact ttttctgcca ccattatacc
     2581 cccatttaca ctaattttca ccactttacc ttatccattt aaggtactat gtcttgctgg
     2641 tattatacta tattttgcca tcattgtgta gacaagctat tttaatacca cggagaattc
     2701 acgcacccgc ttctttccag atcctacact cgtaatattt ttacaaaatg ctttgcagta
     2761 ttactaaaac cttggctcct acctatatga tatggtcatt accccgtcat ccaatccttt
     2821 tcccattgcc cttatttcta caagctcgct atcatcatct ctttgtacca ctaacatagc
     2881 acgtccgcgc caagcactgc ctatggcttc cgtatagtct tgcacagcct ttggatcacc
     2941 ggaacccata gccaaaagct ttccgccccg tacatttatt tctatctcgt cctctgcgta
     3001 agggaccaat ctattgtgct catcagctat ctctacctct ataaaagcca tattatcctc
     3061 attatctcta tggttttcgg acctaaagat tattttttgg ggcctaccta cggtacaaag
     3121 ggaggctctg ctaacttcta acccatccgc gtaagctaca gcttccagct tccccggtat
     3181 atattgaatt tcaaattctt gtatatacct agtttttgcg ccgcagctct ttttatccag
     3241 caattcaccg tttagataca gctcaacctg atccccagct gcatatacaa ccaccttaac
     3301 cttagagcca tcatacccgg gaaaagacca gctctccagt acgtccggcc agccccatgg
     3361 ggatattttt tcttcctttc cccagtcctt tggatgccaa acccctatgt agggcttatt
     3421 ccgatcattc cataaaaaat ccctatagtg tgattgaggg cgtttcctac cgcatatatc
     3481 aagatcgccg cagttagcta catgccacgg gaacttctta agtcccctgg cctccccctc
     3541 gtaggaagta tggccgattc ccgattcccc taaataatcc caagctgtcc agacaaaatc
     3601 gccgattaca tggggatatt tcttaacggc ctcccaattt tcataagcct ctataggaaa
     3661 tgattcggta ccggaaatca cccgctcggg gaaaatcagc gctccatggt ttgggatccg
     3721 gaaccttaac cgttacagca gatttatctt tcacccctat gtcttccttc gtagctatta
     3781 aggcagcatc ccggtcaaaa attttatgta ctatgttaca ggcccgcttc tccggattat
     3841 aaattttagt tttaatctta atatcagcgc tagctgttcc caaagtatcc gttaacgttt
     3901 caacatgaat gccccaaggt tcaatggatg gtatggaacc cgttaacatg gtgacctttc
     3961 gatatatccc ggcgccggta taccaccggg atgcattggg caaagatgcg tttaccttaa
     4021 cctgtaaaat attttcccct tccctcagcc gccccgtaag atcggcaaag aatcccgtat
     4081 atccaccctt gtgggttact ataaggtccc gatttaagcg cacttcacac atacgataga
     4141 cgccgtcgaa atagacgagg gctctttggg acagcatatc cccggctata ttaaaaacct
     4201 ttttataata gccgataccc ccggggtaga atccttcgct ggggcctgaa aagggatcat
     4261 cacaccgctt taacccgatt ttaaaatcat ggggcagatc aacgggttca ttggcatggc
     4321 ccccatattg gtttacccaa tcctcgttct cggcataagc aaaataccaa tcctttgaaa
     4381 agtctagaat cttaataaaa acccctccca tcaattatta acaattggtc ttagtcttta
     4441 caggtattat ctccacgagg ccatcttcat tcatgctgcg gttgatccct ccctcgatgc
     4501 tgtttcctac agagggagct gcagcttatg gtgaattgga tactctgagt gaaaccgtac
     4561 tctaatttta tatcaaaata cctaaaataa aaagcagata aagcaaaaga tcatcaatat
     4621 aacatgaaac tatggctctt ttttgaagca tacatatgat ataattgggg ttagactcag
     4681 gttaacaagc gatacataat ctggggatat gaaaggaagg atgcaaagca aatggaactt
     4741 actttttgtg gcggagcggg agaagttgga gccagctgtc tgcttttaaa tattgacggg
     4801 aagaacctcc tcttagattc tggaatcagg atgggcgatg ccaaggattc attgccggat
     4861 tttcggatta tccaggaaaa ggggggtctg gatgccatac taataagcca tgcgcatttg
     4921 gatcataccg gatccctacc cataatcagc agggaatacc caaacagcgt tatctatatg
     4981 acccatgcaa ccaaggattt ggtgcgggtt ctcctctatg acagcttaaa gatcatgggc
     5041 tacagcgaag gcgagattcc catctacgcc gaatcccatg ttcaggatat gctaaaccgt
     5101 atcagatgct tttccccgga atatacaatt gatctttttg aagattccaa ggttagggct
     5161 acattctacc acgcaggcca tatcgccggg gccggctcca tctacattca gggtgaggaa
     5221 ggaagcttct tctactccgg tgatttctct atggccaacc agcacacggt cggcggtgcg
     5281 gctattccaa ccctaaggcc cgatgtggct gtttttgaat ccacttacgg ggacaagctc
     5341 catgccaaca ggcaaataga agaggagcgg ctggtagcta ccgttcaaaa ggtaatagag
     5401 cgccaaggca agattttaat accggccttc gctctgggga gagcccaaga ggtcatattg
     5461 atccttaaaa gggctatgaa tcggggcaga ctccctaagt ttaatgtata tgtagacgga
     5521 atggtccgtg agatatgcag gatgtatcgc ctaaatccca actatttaag gccttcacta
     5581 gctaaaaggg tatggcggga cggcgagatc ttcttcgatg attggataca atctgtcgat
     5641 gatagggaga tgcgggataa aatcgtttca tcggatgagc cgtgctgtat catctccagc
     5701 tctgggatgc tgacgggagg tcccagccag tggtacgccg agaaaataat atccgacagc
     5761 aagaacttta tcgccatcac aggataccaa gatgaggagg cgccgggaag acagctcctt
     5821 aatctgctgg atgatacccc ggaagatgcc tttttaagta tcggggatcg caaactcccc
     5881 attaaatgtg aattgggcaa gtataaccta tccgcccatg ccgacagagg ggagatcata
     5941 ggcctggccc acaatataag ccccaaggaa atctttctgg tccatggtga ggggcaggcc
     6001 atgggagagc tgggcaggca tatgcaaggc gacgtgcggg gcagggtata tatagccgct
     6061 aacggcgaaa gctacgatat caatatcgga aaggcccgca ggcagcgccg gtttgcttca
     6121 ataccgaccc ttggtaaaga aggattgcca cagggggatg attttataac cctgtgggaa
     6181 cacataatag ataaccgtgg cgataggtct tcctatacca gcgaagagct aatccgcata
     6241 tgggcggata aaagcagttc ggaagaggat acaagggatg ttatgaacgc catcagccaa
     6301 tcgctgtact ttgaacagga ttatcgccgc ccctttttat tccggcccgt atcccaggat
     6361 agcataaatg aacagctaca ggagatggaa ggccctatgg aagtaaatca aatgctgggc
     6421 ctggtggatg aatacttccc cccgcaaacg gggctttata gaaggggagc aaggtttgac
     6481 gaaaagatag tactcctcta tttcaaattc ccccttatcg cccaaaggga gtttaaagac
     6541 aagtttcagg agtttgaatc cattacgggt tggaaggtgc agatcaacga ggagtacaac
     6601 ctcgaagccg ctgaggatct attgcatacc ctaatgccta cacaaataga ttggtcaggt
     6661 aagctctcct actataggtt ggaaggggtc tttaaaactg ccatcgaggg ggatatagat
     6721 ctgggtaagg tatgcgagga ttttgaggcg cgaaccggac tccccctcat catagaagat
     6781 ggaaagggtt ccctacctct agagccggta tcggcaaccc atggtcagat ggagcaaaac
     6841 aaggctttcc agatcatcga tgaggccttc gcagggaaac ccgtacgcgt atataagaaa
     6901 gggttaaagc gggatgggga taccccatac atcgaattgg cctttataac cccagagatc
     6961 ggcgagaagt ggcagcatct catagaggaa ttggaaacac aaacctggtg gaatatacgg
     7021 ataaatccca gccccaacca gaatgaagtg cttaggctag caagacgtct cctggaagga
     7081 gaaggcctcc tcctcaggag aaatcccagc tttattcaga tagatcaagc tgtaagggtc
     7141 actccggccg aaccttggga tcccgaggtc cttgaggaga taaaggacgt cttcgccggg
     7201 gaaacggggt ataccttggt tgtttcgaaa tagaggtaga aagcaaattc cctttgtcgg
     7261 ctcgcatctt tctgtattgt tgcccaccgt ttttaacgag actcgggatt tttgccgccc
     7321 cgccctttgt aatccttttc gatctcgtcc ttccaatcct ccgatactga attggactcc
     7381 cgatagtttg ccactacatc gctcatcacg gaaaaggtca aaccaacgcc ggcgctgtct
     7441 gcatggaaat tttgtgctcg attggccctt atgccgaaat gcccggccgt ttctactgcg
     7501 tcgatgttgg ccccaagaaa gataaactcc cagccatact tttcttcctg cctttcaatt
     7561 agggccttta ccttccccgc cgaatactca cggctggcgt tttccatacc gtcggtaata
     7621 atcacaaata tcaccttttc agcctgatat tcttttgcgg tgcttttctg aacgctgatt
     7681 atcttatgga cggtcctgcc tatggcatcc aaaagggctg tagtcccacc tacaaaatat
     7741 tccttttcgg tgatggggct gacggccctg atgtctatgc ggtcgtgtaa tagctcataa
     7801 ttattgtcaa agagtaccgt ggtgatacgg cattcccctt ccacttccct ctgcttttgg
     7861 agcattgaat tataaccgcc aatggtatcg ctttccagtc cgctcataga cccgctctta
     7921 tccaatataa gtaccaattc tgttaattcg cttttcatga tacgtaccct ccttaaggtt
     7981 tttgtgcctt aagaataaca cctatgaggg tctgcagggt cgctttggaa gcgacaatcg
     8041 gccggagatt attttttccc attacagaat cattcgcatc ttctagtttt gtgttagagc
     8101 aatgcatctt tcgctgaata tgaattaagg ataatgtttt tcgatgtttg aacaacaata
     8161 tagaatgttt acagtagaca tatagaagaa aatcctcgaa agggtataaa accgaaagtt
     8221 attctatatt acttgaaact tattttgctt ttctgtgtat ctattattgt agggccggca
     8281 aggggtggta cggttgggag ctgaagaggc gaaaatcata aagctttgca agggcaataa
     8341 aagagaaggc tataatttgt tgtacgaaaa atacgaagcc tatatctact ccctttgctt
     8401 taggtatacc cgttccaagg aggactccct ggacctacta caggatgtat atcttaaaat
     8461 atatcaggga atgcccaggt ttaaggaagg gcgaaacctg cttccttgga tccgaaaaat
     8521 aacgatcaac acttgcctaa actttgtgac cagaagaagg gataacgccc tatctttgag
     8581 catgcaaata gacgagaacg aaaataccct ggaagatatc ataccgtccc ataccagcgt
     8641 agtagacgag ttcttatcaa taagggccaa aaaaatacta aagagcgcca tcttgggact
     8701 accgaaggac atgaagatgg caataatttt aagacatatg gaggctatga gctacaggga
     8761 aatagcaaag gctatgtcga ttccgattgg tacggtcaag acccttctct ttcggggtcg
     8821 gaaaatatta aaagggcagt taatggccca aggggtctgg gaggagtaaa atgatctgtt
     8881 gtcttgataa ggatattctg caggaaaagt tggatggctc tttaggccat gttgaggata
     8941 tgctcctaag tgaacattta aggtgttgca aatcgtgcag cagggaatac agtcaaatgt
     9001 cgtcagtttt tgatgggatt gatgccataa aaggtccgat tgatataccc ggcggccttt
     9061 tccttatcag aaaccaggtg ctaaacagga tccccgaact cgaaagcagc agtacctata
     9121 gcctgggtga atttattcgt attcaaaaaa cagccgttaa gagttcaggc aagtttttag
     9181 aatatgttcc cggtacaaaa tatgtagaag cgggcataaa gaaggcccca aagatcttct
     9241 atagggcgtt tagttttgct atgatccggg gaataaaatt tgcgacaggc aggtcaagaa
     9301 catgataata ttgcagattt ttatctattt gttgctattt ttaatatcta ttctaatact
     9361 cctgctcgcg atgcccatat attataccct ctcgggtatc aaagaggata tggtgattat
     9421 aaaggcccaa attttcggac cgctaaagtg gatatgctta accttcaaaa tgcgggatat
     9481 gacggagaag aaactgggtg taagattcct tggtataccc ataaaaatca aggacaataa
     9541 aaaggatgcc ccgatcggaa gtaaagtaaa acggaataac aaaggcaaga aaacccgaaa
     9601 aaagggccga aaacccgccg aagggattcc ggcaggaata gtcaatctaa tcgtccgggc
     9661 cctgagacat ataaaacccc gacggcttga ggtaaagggc agatatggct ttaacgaccc
     9721 ctaccatacc ggaattgcct gcagtctgat taataccatc atcccccata aggacaaata
     9781 taatgtggat ctgatcccgg tgttcgatga ggaggtaata gagggcaaat ttttaataga
     9841 aggatacatc gtacccgtgt acttattctg gctattcatt aaggtgtttg tgttgtccag
     9901 gcttaagaag gcgtttaata agtcttttag ggggcgattt aaaagatctg tccctgccca
     9961 ataaataaat agaatgaaag gaagtatgat tatgtcagtc aatgtgaatg taggagagaa
    10021 tatagacttg ttatttcaga acctggagga tttcattaaa aataagacca tatttggtga
    10081 acccatgcaa ataggtgatg ccaccttaat ccccgtagtg gacgtctcat ttggacttgg
    10141 ctccggcggc ggagacggca gcgataataa gggaaacggc ggagtaggcg gtggggctgg
    10201 aatcggtgcc aaggcctccc ctaccgcaat cattgtcatg aaggacaaca atgtggaggt
    10261 gctgcctata agaaaaagtt cgggtcttga aaaactcctg gaaatggttc cggacctggt
    10321 tgatagggta agagccgaga tgccgacaaa gaataaagac gaggataagg aataaagaat
    10381 tagctaaacg cccctgtttg ctatggcagg gcgtttaata tccattgaaa tcccttaaat
    10441 ccgtcgcaac tattttatca caccagtttt tgcccataat aaatgcccgg caggctctag
    10501 agtcctactg ggcatttgtt ttggccccag tcttttacca tatcattctc ctgttgtgta
    10561 ttgtgtttgt agtcgaggga attgtcccgc ggcgaaaaga cccgccggct ctctccctat
    10621 cccatactat ttgccgtttt cctataaccc attcttcaat aacagatccg ttaaccaaat
    10681 gcctgtatac tatttccctt gccctgtccc ccgaaacctt cccgtagatc accgaaggtt
    10741 ggcccgggag gattacctct acgatgggct ctaactcgca gagaccaatg caccccgctt
    10801 gggttacttc aacgtcaaga ttcctgttta tagtgtccgc cagaaaagcc ttcattgttt
    10861 ttctggctcc cgccattatc ccacaggtcc ccataccgac aataattctg accttgcctt
    10921 ccttttcatg tccctcttcc tttttcctaa tctcttcacg gattctgact aaatccccga
    10981 gggatcccat ttaaactacc cccatttcat ttttagctga aaattgatta aacgatatta
    11041 atttgatcaa gtaatattaa ccggattctg atttgaattg taatcctact atcattatat
    11101 cgcctcggac cttttagtgt caacgcctat tgactgattt gtctaactat ttgataagct
    11161 tagggcatcc tggccctcac gggaacattt cccttgtaaa acatggcagg tcggggctat
    11221 aataatatag acctgtcctg taatcttggg ctgaaattta ctgaagccca tgattcaaga
    11281 ccacggttga agatcacgat tcaagactgc ggttaaagat catgattcaa gactgtgatt
    11341 gaaaataacg ggtctgactt tttacattga acttgatcca ttatattccc cataaacgac
    11401 gttttaataa gatcatatgg cccccaggag gtgatgatta tggattcccc ccatactaca
    11461 tatgcaaggc cgggcgagat gatgtccggt actttcttaa aaatcatcgc gattatggct
    11521 atgctgaccg accatatagc atgggccttt gtacccaccc atagcttatt gggtcagttg
    11581 atgcacgttg taggtaggct tacggcacct atcatgtgct tttttatagc cgagggctac
    11641 caccatacgc gaaatgtaaa aagatatgcc cttcgccttg gccttttcgc ccttatttcc
    11701 catgcaccct atatctattt cgaatcgggt aggcctccta tctattatga aaatggtata
    11761 aaaataatct atggaaccag cgttatttat acccttcttt tagggcttat tctccttatg
    11821 atctggaatg atgaaaggat taaagagcgt gtgaagcacc tcctggcgat tttgattgtc
    11881 gtagcctccg ccccggggga ctggtcattt tttgccgtcc tatggatatg ggcttttggt
    11941 gtacaccacg ggaatataaa aaagcagctt caaagcttca gcctcatagc catactgcct
    12001 gtcaccttca gcatcatctt attttcccaa ggcgtttggt ggggccaact gttccagctt
    12061 ggagtatacc tggcgatacc ctttatctat ctctataacg gaaagctggg gtacaaaaaa
    12121 ggatgggtct ggattaaatg ggttttctat gcattctatc cccttcatct tcttgtactt
    12181 ggtctcttaa aatacatagg gcgttaatgg aggtttctat ccctttaacg cccgtatatg
    12241 tgcttcggca tgcataagga tacatactct aggactatgt attctagata tgttacgaat
    12301 aggacctatc aataggtcaa tagtatcgat tctatgtact atcttggccc tgaataaccg
    12361 tcataatgcc ttctactggt tatcgtcttc cctattcatc ttccttttca tagtccgggg
    12421 gaagctgtac cgtagccttt ttaatgactt gggcctctgt gggataatcc ctcatatccg
    12481 tgggcgtctt agctatggca tccaccacat ccattccctt gattgcctta ccaaaggccg
    12541 catatgcacc gtctaaatgc ggggaatcat cgtgcattat aaaaaattgg ctccctgcag
    12601 aatcgggatg ctgggacctg gccattgata taactcccct tagatgttta aggtcattat
    12661 cttcgaatcc attgtccgca aattccccct taatgctata tccaggaccg ccggtccccg
    12721 ttccttcagg gtccccccct tggatcatga acccggggat gatgcgatga aaggtaaggc
    12781 cgtcataaaa cccgtcctct accagggata caaaattggc taccgtctcc ggtgcttttt
    12841 ccggataaag ctctattact atgtcccccc tccccttgat cttcatggtc accgttgggt
    12901 attttacatc tttctgggcg catcctgcca tgctcccgat tcctaaagcc aggatcaggg
    12961 tgagcaatat gggatgaacc ttaaacagta tctccataac ctttctcctt cctacgcgac
    13021 tttgtgttta gtctctcttt aatcggatgt tttataacaa tagtcatcat tatacatcat
    13081 agggcccgcc tgtgtaaatt gatttcactt cgtgctatgg tatataatag cagtatagca
    13141 aggttttttg aatgaggggg tataaatttg attgtaggcc tggatatggg cggaacccat
    13201 atagatgggg tcataattga aaatggaacc ataataaata ccgttaaaaa gcccagagcg
    13261 gatggggata tattcggatc catttggggt gcgctggaag ggctcctatt agatcaggac
    13321 acgactaaaa tagaaagaat taacctaagt actaccgtat ccaccaacgc catcgttgag
    13381 gataagacta cccctgtagg catggtcatc cagaccgggc cggggctttc cggtgacttt
    13441 ttagcttgcg gcagcgaaaa tgtctttatc tcaggctgca tagaccatag gggcaaggtc
    13501 gtcgagagat tggactccaa ggaaattgac agagccaaag aattatttat cagagaagga
    13561 cttagtgcct gcgcggttgt gaccaagttc agtaccagga atttcgagca tgaggtccaa
    13621 attaaaaggc ttctggggga tgggtttgac cacattacca tgggacatac cctgtccggt
    13681 atgctgaatt tcccccgtag ggtctttagt gcctatctaa acgccgccat ccacaacacc
    13741 tttaatcggt tttcccatag catcaaaaag gctatgatag aaagggaaat taacgcccca
    13801 ttatttatac taaaggccga cgggggtacc accgttttag ccgaggcgga gaacaggccc
    13861 atcgagacca ttttatccgg tcctgccgcc agctttatgg ggcttaacgc tctgcttaag
    13921 acccaggagg atgcgctgct tttagacatc gggggtacga ctacggatat cttctttctg
    13981 gcggacggcg tccccctatt cgaacctatg ggcattgaga tcaattccta caagacccta
    14041 acgagggcca tctacagtgt ctccatggga ctcgggggag acagcagcgt tcgtgtagaa
    14101 aaccgggaga tcatgatagg tccaagacgg gaaggactac cttatgctct ggggggacct
    14161 tgccctacgc ccaccgatgc catgatcgcc ctgggtttgg tggaatttgg cgatagaaaa
    14221 aaggcgacag ccgctatgaa atccctagga caaatcctgg ggttatccgt ggaagaaacc
    14281 gccagggata ttctggatat catggccgac atgattaaat ccaaggtaga cgagctgtta
    14341 gataagatca atgataaacc tgtctacacc gtacacgaac tgctccatgg caggaagcta
    14401 agacctaggc ttataaacct tatcggcggc ccctccaagt acctgagccc tgtaatcgaa
    14461 aaagcgttcg gtctgccctg ccattatccc aaggattacg ctatagccaa tgccattggg
    14521 gcagctatgg cgaagatgac ccaggacata acccttgttg cggatactga gaggcgcctg
    14581 cttaccgtcg gagagttcgg tgtcagggaa aggataggaa ggggctttac cctgaaggat
    14641 gcccggaagc gggctattgg gctcctggag gacagggctg tcgcccaagg cgccaataaa
    14701 ggggaaatag aaacggaaat cattgaagag aacagcttta atatggtgga cggcttttac
    14761 acggtgggga aaaacatccg agtaaaggcc cagataaagc cgggccttgc aggggatcta
    14821 aaactttaaa agctcccaag aacctaaaat agcgataaag gatgatatgg ttgattaaag
    14881 cgaaaaatag tttagggctg gtcttcttcc ccgccttcga ttgggctata agccctaccc
    14941 atccggaacg ggaagaaagg ttactgtata ccagggatca gctgttcgaa gaggggattt
    15001 cagacattga ggggatccat ttttataatc ccatcatcgc ccatgaaagg gatatccaaa
    15061 gggtacactt ttgtgttccc gatgttgcct caaggttaac ggattcacac ctcatcgcag
    15121 ccggtggcgc catcaaggcc atggatgctg taatgaaggg ggacgtggat aaggcctttg
    15181 ccatggttcg tcctccggga catcatgccc aaagggttgt ctatggcgac aggggttttt
    15241 gtgttattaa tgtcgaggcg gtgatgttgg agcgactaag gcaggattat ggggatttga
    15301 aggtcgccat aatagacacg gattgccatc acggggacgg gacccaggat atatactgga
    15361 acgataaaaa taccctgttc atatcctttc accaagacgg caggaccctc taccccggat
    15421 ccggattttt aggtgaattt ggcggaccag ccgcctatgg ctacaatata aatatccctc
    15481 tacccacggg aaccggggac gaagggatat tgtatgtgct ggaaaacgtc gttcttccca
    15541 tccttgaaga ttacaagccg gatatcatca taaattctgc gggacaggat aatcattata
    15601 ccgatcctct aaccaacatg aacttcactg ccgggggcta tgccaggctc aattctaagc
    15661 tgaacccgga tatcgcagta ttagagggcg ggtattccat tgaaggggca ctgccctacg
    15721 taaacctggg tataatactg gctatggccg gccttgacta ttccggcgtt agggagccgg
    15781 attacgacga ggaagccctc aaacagaccg aggggaccac agagtatata aaggagctgt
    15841 cccaccttgt atacagccgc tggcagaatc gggaaagact atgggccgaa gatataaagg
    15901 gcaaggatat tgtggaactt gaaaggcagg tctactacga taccgacggc atacttgaaa
    15961 accagaccca gacctttata tcttgcagaa aatgttccgg agtcaacacc atagattcta
    16021 gcagcgatgg cggatatcat atctatgcca taaccatacc aagggacgcc tgcgccgagt
    16081 gtatccacaa gggccacaag atctataagg aagcatcccc gggagattat acgaatatct
    16141 atttgcagga cagggtaaac gatacttgtt tttcaaagta gggcttttat gccctgtatg
    16201 ccctcaaaag tccttcccaa catttggcga ttttttcggg ggtatatgtt tcatcggtgt
    16261 ttttatggga gagttcaaag gtaaatgggc ctacgtagcc cacttccgtc aaggcggcaa
    16321 ttacatcatt ccagtccacg atgccctctc ccggcatcca atggcgctca tcgatcccgt
    16381 caaaatcgga aatatgcagc gtcacgatcc gggatcctat ccttcttata aaattccccg
    16441 gcgactcttt aaacatgtgg ttggtatcac agcagacacc tactttatcg tttccccgta
    16501 tcaattctgc catctcttcg ccggaattcc ccaggcatgt tctgggtagg cattctatgg
    16561 caagttctat ccccaaggtt gcggccgcct cccctatagc ccaaagggaa tccctgcatg
    16621 ccgatattcg ggcatcccgt tcgttatctg aaatgggctc gaagctcccg tggataaccg
    16681 ccttttttgg ttccattact gcggccagtt ccagcagccg gacctgtccg tctatagcag
    16741 attttctttt atcgctatcc ggctcggata tatcccactc ccggccaaag ggcaaatgta
    16801 ttgaccaaat atttatgccg gcattatccg ccttgcgctt tatatcggcg caagccttgg
    16861 ataccttttc gtcgggttct tgtgtccagt ccccgaagag gcatacctcc atgttttcta
    16921 tacctgcgtc catacactct ttaaaaacag cttcatcaag ggaagaaaac aacgatgatg
    16981 atgtccccaa accccatgat gcggggtcac tggtcacgat actgctgaat ttgtcattat
    17041 ccttaatacc cataacgatc tataccccct taattatggg ccggggcgtc acaccctacg
    17101 gccctgatta aatatatcat agcgattatt accatataat acaaggccaa aatactgttg
    17161 acattgacgt tgggttatac tttacaatag ttatcacagg gggtgatgat atggatctta
    17221 agataaagga agtcgctgat ttggtaggga tcagtgtgcg cacgctgcac cactacgacc
    17281 aaatcgggtt gctgaccccg acaaagagga ccgaatccgg ttatcgcctt tattcgggca
    17341 gcgatcttga ggtgctccaa cagatgctgt tttttaggga gctggacttc ccacttaaaa
    17401 agataaagga gattattgag agtccatcct ttgatcgacg ggaggcgctc ctcctccata
    17461 aaaagatgct ccttgaaaag cgcaatcgca ttgataaaat acttgcgact gtcgacaaga
    17521 caataaggta tatgaaaggg gaaattcaga tgtccaacaa agaaaaattt acagggttcg
    17581 attttgggga taacccatat gaaaaggaag cccgcaggcg ttggggcgat gatgccgttg
    17641 aaaaatccaa tgctaaaatt tatggtatgt ccggagccga aagggaggcc atgggcgata
    17701 agatgaacgc catctatcga aggttggcgg cgcttaggga tactccgccg gaatcggacg
    17761 atgcgcaaac agccataaaa gaatggcacg actttttaaa tcaaaatacc ggacaccatt
    17821 attctttgga tgcattcaaa gggctgggcc agatgtatgt aggggatgaa cgcttcacca
    17881 ataatatcga taaatttggt gagagtttgg cagaattcat gtgcgaagct atggctgtct
    17941 atgcggagaa gaatagataa tagtatcgtg ttatattagt ggtatattat cagtagggat
    18001 ctaaaaacct tatagtccgg gggaggttgt taaacagcta tctgttggag gatcttaaaa
    18061 cttcgcagtt taggggttta gttaaatatg agattcgttg gcccatatgc catctatagc
    18121 cccaatctat cccattattt ttatgtggaa ggtatgaagc gcggataatg atttcaatat
    18181 aggaagtaca aagcgtgaac gaaaatttaa tggaggaggt ataaagcatg gacaatagtc
    18241 ttagatctat aaaatacata gatctctcac aggataaagc tggacaatac ccggtagaca
    18301 aagaggagaa tcaatacctg ggccatccgg attctatact cttagacgac ggaaccatcc
    18361 ttgtattcta tcccaaaggt catggcaaag gtcctattgt catgaagaag agcgcagacg
    18421 gaggaaaaac atggggtcaa aggttaccca ccccaaagag ctgggccgat agccaagaga
    18481 cccccaccgt ctatcaggta ataaagcctg acgggactaa gaggcttgaa ttgataagcg
    18541 gcgtgccctg gaatgaagga ggatttaaga cctcttattc agaggatgac ggacagagtt
    18601 ggaccgagtt tgagcaccac ttccccggac ttcataccat agtagcccat gccagcctca
    18661 caagattgaa agatgatggt ggaaattggg ataataggtg gatgggtctt tttcacgatc
    18721 atgaattcaa taattggatg agcatcttaa gtttcgacga tgatgggcaa gggcgatgga
    18781 gcagtcccca gcggttgctg gaagaacatg acgaaataga aaagcatgca cagatgtgcg
    18841 aaatagaggt aataagatca cctgatgggc gacagttggc cctactggca agggcacagc
    18901 gcaagataac caacgccata gtggcattct ccgacgatga gggccaaaca tggagccggc
    18961 ccagagaagt cccctggccg cttatgggag agaggcataa agcccaatat gaccccgtat
    19021 ccggtaggct gctaataacc tttcgtgaga tcatgagaaa ggaaagaaac aatggggatg
    19081 aaaaatggat cgccggtgat tggtgtgcct gggtgggtac ctatgatgat ctatacaagg
    19141 gcaggatggg agaatattat atcaggctca tccaggattt tacaggggat tgcgggtatg
    19201 ccgggaatat agcgctgccc gacggcacat ttgttctcac gtcctatgga cattgggata
    19261 aagattacca caacccctat gtaatgacgg tcagattgaa gttatcccag ctggatgaaa
    19321 ggttctccca cctcaaattt aataaccctt atcataatcc cttatattga tcatatcatt
    19381 cgtcctgcct tcgcagaagc atttcacgtt atgcattatg atattggtgg aaaagtaatt
    19441 ccactggtcc ccggcgcccg ccatatgggg cgtaataata agattatcca aatcccagaa
    19501 ggggctctcc ttgggcaaat atcccagcct aaactcatcc ataaatacgt ccaaggcagc
    19561 accggctatt tttttatcct ttagatagct gtataggatt tcttcgttga aaattatcgc
    19621 ccttgatata ttgattatgt ataccgtggg tttcatacac ttaaattgct cttcatcaat
    19681 taaattttcg gtcctgggtg tcggcggaag tgtacacaca ataaaatcgc attgggctaa
    19741 aaattcgttt aatccgtcgg ccgaatagac cttatcgaaa tattctaacg gttcgccggt
    19801 tgtattatac ccgattgctt tcataccaaa cgccttggct cttttcgcag tttcgctccc
    19861 cacatctccg gtacccaaaa gccctacggt tttattatag aggttaaata cgctgccggg
    19921 gcttttccat tcacgggcaa gctggttttt aaatgagggc aggctttttc tggcaagcat
    19981 caacatcatg gtcatgatat tctccgatat gggtacaccg gctgtcccac gggcgttggt
    20041 cactacgata tttctttctt ttagatattc catagggagc ctctcaatac ccacatgggg
    20101 aacgtgaatc catttcagct ttttacactt atctagaaaa tctatctgca gggtcctgtg
    20161 gttagcgata atggcgtccg cttcttccac cttatgggat tgaaccaatt cttcctcgga
    20221 agaaaatacg gttacgcatg cgccgagttt ttccattttg gttatatcct ctttaccggg
    20281 tacaaatgtc gtcaataatt tcatatctaa ctgtcccctt cctcttcttc ctttgtagta
    20341 tgtcgccgtc tcgggggtat ttcccaatcc cttgaatctg ttttaatcaa aaggcccccc
    20401 taacaatacc agcccaaaga ttatgatatc tgttgctggg gaataggtcg agtttgttat
    20461 tactttttaa tgtgacacaa tgtcatgggc aaaatcaacc tatctgctta tagcatagtg
    20521 aagcaggggg cttaagtcaa gga
//
LOCUS       DASRNX010000068         4803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_702563,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000068 DASRNX010000000
VERSION     DASRNX010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702563"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFD89_02780"
     CDS             <1..616
                     /locus_tag="VFD89_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZJ57145.1"
                     /translation="YSGEIRILGMSPRKAHGIIGYVPQFTRFHRDFPISVEETILMGR
                     LSGKTPIFHKYGADDRDTAHSLMEKLEIYPLRNRQIGQLSGGQLQRALIARALTTKPR
                     ILMLDEPTASLDTEAKSHIYSILEELNSDMTIIMVTHDMGAISAHVESLACLNRELFY
                     HGRSELSEDIINGIYGCPVDIIAHGVIPHRILKEHHESGGNGHD"
     gene            609..1424
                     /locus_tag="VFD89_02785"
     CDS             609..1424
                     /locus_tag="VFD89_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZJ57146.1"
                     /translation="MINSLIQYKFLQNAVISAILASVACGIIGSIIIEKRLVMMSGGI
                     AHTAFGGIGLGFFLGIEPIVGAIFVTLCAAMGVVAIKRKTRMNTDIPIGIFWAAGMSL
                     GILFIALSPGYPPDMNSYLFGDILMVSRGNIYIMLSLDLLIVFTVAALFHPLKAYLFD
                     EEFSTVIGIKTAFIEVLLFALIALTVVVVIHVIGIILIMALLTAPPAIARLFTHDLKR
                     LMLYSILLGAFFTLSGLWISYALNIPSGAAIALFISLVYGAVSALRALTQKKR"
     gene            complement(1459..2253)
                     /locus_tag="VFD89_02790"
     CDS             complement(1459..2253)
                     /locus_tag="VFD89_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZJ57147.1"
                     /translation="MKHQFTIGTFNIRYGLADDGINIWDNRKELVIDSIHDKLPDIIG
                     FQEALPFVKEYLDKNLSDYIIVGTGRDKDLGGEDNCIAIRRETFELIALETFWLSPEP
                     YVFGSQFENNDGLPRICTMVTLRSKINNRIIRVFNTHLDYRFSDVRLKQLKVLNEFIK
                     QYDDRMPLPNFLIGDLNSVPDSKEIEYVLEDFAVKMVDISTSDQIKSDTTFHGFFNGL
                     DYVGKIDFIFATPDINCIKSYIDDTEREGVYLSDHYPVYAVIEMES"
     gene            complement(2419..3363)
                     /locus_tag="VFD89_02795"
     CDS             complement(2419..3363)
                     /locus_tag="VFD89_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZJ57148.1"
                     /translation="MDVVGIDTPCVDLLANIPKIPEPNKGTRIQDYSWQGGGKVSSAM
                     VASARLGAQCGMIGVVDGDAYGKFCLDDFKDHGIDVSRMIVDRDGETPFSIVLADDHT
                     HGRSIIYYPGTNRPLEVSDLDKDYIQSASYLHLAHDTPVTKQAAIWAREKGRTVVYDA
                     DGYRPGIEDMIPLIDVFIASEFFYEAMFKDGDYEANCRKVQDMGPDIVVFTLGDKGCV
                     GVQGNEFFMEEGFSVEVMDTTGAGDVYHGAFIAGLLQGWDARQTARFSNAVSAIKCTR
                     MGGRAGIPTFDTTMEFLETGVIDYSEIDKRVARYRKSI"
     gene            complement(3603..4391)
                     /locus_tag="VFD89_02800"
     CDS             complement(3603..4391)
                     /locus_tag="VFD89_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZJ57149.1"
                     /translation="MKKGVQTFTVRDFMGSREEVESSLRKIKEIGYDSVQTWTPSFMT
                     PEEHKALLDDIGLETCSSGGDYEQMLENPEAIEKAIEQAKIYGVEYIGVGTLPREMRE
                     SESGYRQYAENINKIASQLKKENLKLLYHNHALEFFSLGGGLMGMDIILSDTDPDGLH
                     FTLDTHWLTAGGVNVVDWIYKVKGRMGIVHFKDYAIVGGAVKIEDVHKNFAEVGEGNI
                     DWPNVVKACKDIGVEFVIVEQDICPGDPFDSLKISFDNMVKFGV"
BASE COUNT         1272 a         1214 c          930 g         1387 t
ORIGIN      
        1 ttactcgggg gagattcgca tattgggaat gagcccgcga aaggcacatg gtattatcgg
       61 atatgtaccc caatttacca ggtttcatcg tgattttccc ataagcgtgg aggagaccat
      121 tttgatgggc aggttatcgg gtaagacccc gatttttcat aagtacggag cggacgatag
      181 ggatacggcc cattccctta tggaaaagct ggagatttac cccttaagaa acagacaaat
      241 cggccaatta tccggaggcc agctccaaag agccttgatt gcgagggcct tgacaacgaa
      301 gcctaggatc ctaatgctgg acgagcctac tgccagcctt gataccgaag caaagtccca
      361 tatatattct atattggagg agctgaattc ggatatgacc atcatcatgg taacccatga
      421 tatgggggcc atatccgccc atgtagaatc tctggcctgc cttaacaggg aactattcta
      481 tcatggccgc tccgaactgt cagaagatat tataaacggc atctacggat gtccggtgga
      541 tattattgcc catggggtaa ttccccacag gatattaaaa gaacaccatg aaagcggggg
      601 aaacggacat gattaattca cttatacaat acaagtttct gcaaaatgcg gtaatcagcg
      661 ccatattggc cagcgttgcc tgcggaatta tcggatccat cataatagag aaacgattgg
      721 tcatgatgag tggcggcatt gctcataccg ccttcggcgg gatcgggctt ggattcttct
      781 tagggatcga gcccatagtc ggagctatat tcgttaccct gtgcgcggcc atgggcgttg
      841 tcgccataaa gagaaagacc aggatgaata ccgatatccc cataggcatt ttttgggctg
      901 caggcatgag cctcggtatc ctatttatcg cactcagccc cggatatccc ccggatatga
      961 attcctacct cttcggggat atcctgatgg tctccagagg taacatctac atcatgttgt
     1021 ccttggacct tcttatagtc tttaccgtag ccgcattgtt tcacccctta aaggcctatc
     1081 ttttcgatga agaattctcc acggtcatcg gcataaagac cgcttttata gaggtattat
     1141 tgtttgccct gatagcactg acggtagtgg tggttataca cgtcataggc ataatcctaa
     1201 taatggccct tttgacggcc ccgcctgcca ttgccagact gtttacccat gatctgaaga
     1261 ggcttatgct ctattccata ttgctgggtg ccttctttac cctgtccggg ctttggatat
     1321 catatgcact caatatccct tccggcgcgg ctatagccct gtttatcagc ctggtctacg
     1381 gtgctgtttc cgccctgagg gccttgacac agaaaaagag gtaggacggt attaatcacc
     1441 tactcccatc gcttcccctt atgattccat ctctatgacg gcatacaccg ggtaatggtc
     1501 cgataaatag acgccttctc tttctgtatc gtctatgtag gatttaatgc agtttatatc
     1561 cggtgtagca aagataaagt caattttccc cacataatcc aaaccgttaa aaaacccatg
     1621 gaatgtagta tcgctcttta tttgatccga agtggatata tccaccattt taacggcaaa
     1681 atcctcgagg acatactcta tttccttgct gtccggaaca gaatttaagt ccccaataag
     1741 aaagttgggt aggggcatcc tatcatcata ttgctttata aactcgttca gcactttaag
     1801 ctgcttaaga cggacatcgg aaaacctata gtcaagatgg gtgttaaaaa cccgtattat
     1861 tcggttattt atctttgacc ttaaggttac catcgtacaa atccggggca aaccgtcgtt
     1921 attttcaaac tgggatccaa atacataggg ctcaggggac agccaaaagg tttccagagc
     1981 tatcaattcg aaggtttccc gccttattgc aatacaatta tcctctccgc ccagatcctt
     2041 atcacgaccg gtacccacta taatataatc ggacaagttc ttgtccaaat attcctttac
     2101 aaagggcagg gcctcttgaa agccgattat gtccggcaat ttatcatgga tgctgtctat
     2161 tacgagttcc tttctgttgt cccatatatt aatcccgtca tcagccagcc cgtatcttat
     2221 attaaatgta cctatggtaa attggtgttt catcttggca tcccctatct caatcattat
     2281 gaatattttc ccgccaggca tatatgatta gctacaaatc cctgggagac cccaaatgaa
     2341 aattcggggt cttcttttat catgtatccg tctgtatgct ttttctatac ctttttatat
     2401 gtttttttct acacccatct atatgctttt tctgtacctt gccacccttt tatcgatttc
     2461 actataatca ataacgcccg tttcaaggaa ctccatggta gtgtcgaagg tgggaatccc
     2521 cgcccttccg cccatcctgg tgcattttat tgcggagacg gcatttgaaa aacgtgcggt
     2581 ctgcctggca tcccagccct ggagaagccc ggctatgaag gccccatgat atacatcgcc
     2641 tgcgcccgtt gtatccatta cctcgacact aaatccctct tccatgaaaa attcattacc
     2701 ctgtacgccg acacatcctt tatcgcctag ggtgaagact actatatcag gccccatgtc
     2761 ctgtaccttc ctgcaattgg cctcatagtc cccgtctttg aacatggcct catagaagaa
     2821 ttcggaggct atgaagacgt cgatcaaggg tatcatatcc tcgatacccg gtcggtagcc
     2881 gtccgcatcg tacactaccg tcctgccctt ttccctggcc catatagcgg cttgcttagt
     2941 caccggtgta tcgtgggcca agtgaagata gctggcggat tgtatataat ccttgtccaa
     3001 atcgctgact tctaaaggac ggttagttcc cggataatag ataatactcc ttccatgggt
     3061 atgatcgtca gccagcacta tagaaaacgg cgtctccccg tccctatcca caatcatcct
     3121 agatacatca ataccatgat ccttaaaatc atctaaacag aacttcccat aagcatcccc
     3181 gtcaacgacg cctatcatac cgcactgcgc gcccagcctg gccgaggcca ccatagcaga
     3241 agatacctta ccgccgccct gccagctata atcctgtatc ctggtaccct tgtttggttc
     3301 aggaatttta ggaatattgg ctaagagatc aacacatggt gtatcaattc ctacgacatc
     3361 catccacact tccacctttc gtaataaatg ataagtccgc tctttatata atcaaccaag
     3421 cccatatccc aaccggtatg attataggct tggttgatta agaaagctgt gatttcaatt
     3481 atccactatt cttgggtatc tttaaagatc acccataaaa gaataatttc aaaaaatcta
     3541 gtcatcgata ttgttgtcta atacagcgaa agatatattc tcaaaatatc tttattgatt
     3601 tcttaaaccc caaatttcac catattgtca aaacttattt tcagactgtc aaagggatcc
     3661 ccggggcata tatcctgctc cacgattacg aattctacac caatatcctt acatgctttg
     3721 acaacgttag gccaatcgat atttccttcg cctacttctg cgaagttctt atgtacatcc
     3781 tcgatcttaa cagcgccgcc gactatagca taatccttaa aatgaacaat tcccatacgt
     3841 cctttgacct tatagatcca gtcaaccaca ttaacaccac cggcagtcaa ccaatgagta
     3901 tccaatgtga aatggagtcc atcgggatct gtatcgctaa ggattatatc catccccatt
     3961 aaaccgccgc ctaaagagaa gaattccaag gcatgattgt gatataagag ctttagattc
     4021 tccttcttaa gctgagatgc aatcttattt atgttttcgg catattgcct gtaaccgctc
     4081 tcgctctcgc gcatttccct gggcagggtt cccaccccga tatattcaac gccataaatc
     4141 ttagcttgtt caatggcctt ttcaatagct tcggggtttt caagcatttg ttcataatct
     4201 ccgccggatg agcaggtctc caagcctata tcatccaata atgctttatg ttcttcaggg
     4261 gtcataaagc tgggtgtcca agtctgcacg gaatcgtatc ctatctcctt gatctttcga
     4321 agtgatgact ccacctcttc acggctaccc ataaaatctc tgactgtaaa agtttgcacg
     4381 ccttttttca taacgtactt ctccaccttt ctttattatc aatcatctgt aaaatcagat
     4441 tagtccacta tatttagacg ggcattaaat aaaacaacaa agtacaatac ctgtgtttat
     4501 tatataagag ctgaatacaa cagtcaatcc gacataggtt ccattcagat ccatcccagc
     4561 ccgacgaact caatatattc ttatgcttct ttaatttata gatcgtaaca aagtctatcc
     4621 atatatgttc aattagtatc caggcaagcc tctgccgcag tcccactcat aaagccggag
     4681 ggcaagggag ccggacgttc acacttggat gtcatctcat aaaccttccc tgtgttacag
     4741 ctctccatta taccgttgat tacttcaaag gtatggaagc ccagctctgc gctacatcga
     4801 tgg
//
LOCUS       DASRNX010000069         7455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_66264,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000069 DASRNX010000000
VERSION     DASRNX010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66264"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFD89_02805"
     CDS             complement(<1..459)
                     /locus_tag="VFD89_02805"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HZJ57150.1"
                     /translation="MRPRDKTIRSVFIVTVFSIAGKLTGFFREAVIASYYGAGGITDA
                     YFVAYSLPSILFSIIGASMGLVFVPIYTQQLKKNPEKSHALASNIINMAFLVGLIFAG
                     LGSAFSRQIIAVIAPRLSGYNMVMAARLARIMFPAFIFISISYIVTGVLQS"
     gene            complement(463..1302)
                     /locus_tag="VFD89_02810"
     CDS             complement(463..1302)
                     /locus_tag="VFD89_02810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZJ57151.1"
                     /translation="MLFLIKTIIKYLLLPLGIIHAVLFRSRDEIIILMYHRVDDGVKK
                     ELAVTRDSFNWHMGYLYRKRYSVISMEKAYSMISNNGIVGRHIVISFDDGYRDFYKNA
                     YPILHRYKYPSIMYLCPGYIESPKKFWWDLDETDAELMEWKEIYELDGDGLVEFGSHT
                     VEHRDMNLVSPAELEREIVESKTMLEKGLQKPVRHFAYPRGIYSKAGEQLLRKHYDTA
                     VLISNGERIVKKGIKQTHALKRIPVLRSDGRYLFIARIKGWLAMEELTRKYFLTRHHR
                     REA"
     gene            complement(1307..3019)
                     /locus_tag="VFD89_02815"
     CDS             complement(1307..3019)
                     /locus_tag="VFD89_02815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZJ57152.1"
                     /translation="MLRSWDKIKPYKAMWDSILRENNTDIVFMTFEWLYLWWKYHGEG
                     KRLFFLVAKDDNNILGFCPLMEVPARGYKEIRFIGGNELSYMGFPIKSGFKCAVLGSI
                     LDYLIGLKGNHMFNIHGIFKDADEYNIFKDYLDKTRRKYLKSSLECYFIGLHNRDYNS
                     YIAGRFGRNSIRTMERKEKRLARLGELSFKPFTERDGDIERIFDIHEKRWQRKVGHSK
                     FSKGRTREFFKELALSDKMPFETSVDIIDVDGTPISFIYGFYYNGRYTFYRIGHDDNF
                     AIFSPGELVLRKKLKDCFEKGYRVFDFGVGYEPYKAAWTDEKADMIGFVFPGDGVLVN
                     LIFNIQRLVRNLRQFLKRDPRIYNFVKYKLGKFKMLLYSGNAKKTFRRGIGRLKNRVV
                     EIVAHIYSSYDYYIVYRPLISPDKADCGIYTVEEASIRKLGLLTEKLGMEPRDVHRRF
                     LDENRCILVWDKKGIYSFWIDTHSIKIPALNFNWNMEGGSTFIYQHFTGRMRSRISHG
                     LLNSVLSYLNGLGYSRCYLVTGGRSDYLMNLTKDVGFSKFKRLRLKKRFGRMSYNKFE
                     DITE"
     gene            complement(3127..4497)
                     /locus_tag="VFD89_02820"
     CDS             complement(3127..4497)
                     /locus_tag="VFD89_02820"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZJ57153.1"
                     /translation="MKGYTGETRLIDKKILAVCTIAALIAGLSPIITGVGLVYISVLI
                     GICAFGALLVFRYHWAVILNILILSFVKFEPAPADLVFILLFAVGVMSHRLESHRLNP
                     TRPMILFPILYILISTLQLFISHEMMLGIRYHLITMYLIAYSYYMFLYVSRDNIKNII
                     RAYIISGTISALLGLAGYLGVLPSLLMYDRYRAMALFKDPNVFGPFLVPVVVMLIEDS
                     WKKQLFDHPSWIRIIHIAINLLGIIVSFSRGAWVNTCACLLIYFVLNIKKIHLPAAEL
                     KRLVLYALLVLVIVSVVWRFIIPHEYKQFLLSRINFQTYDKDRFATHRKGIALASINI
                     FGFGPGQFEYNVLRYSGAEFSAHNLYIRLAMENGLIGLLLFIGSLAFIIYELGIHHLQ
                     GRTYIVMTPASLVAILMGIMVNGIVIDTIHWRHFWFFIGIGMAAITMNHKHNPDSTSV
                     GPPSIK"
     gene            complement(4460..5620)
                     /locus_tag="VFD89_02825"
     CDS             complement(4460..5620)
                     /locus_tag="VFD89_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZJ57154.1"
                     /translation="MGVDKPVNLLMGITLSEMGGAQKVVYDLISSLPRDFYNMTLVTY
                     PGGELIDWVKDLGSVRGADVKIIGIPEIRREISPINDLVTLIKLYKIMKGGKYDIAHF
                     HSSKMGILGRLAAHLAGVPKIYFTVHGWGINEYQPKGLQRLFGWAEKLASKRCSMCIC
                     VSKYHRDLGLKMGWLSREKSTIIYNGIDEAPTTLGKLRNELNIHGDVAIIGTVMRLRE
                     PKQPLYTIQVFNEILSMGHRAKLVIVGDGPLHDSCVEAIESLGISDHVFMLGTRADAR
                     QLINDMDIVTLFSKWEGLPIVIIEALFAGKSVVCSGVGGVSEIIDHGIDGLILEGFDI
                     KNGADQLSVLLQDKGLRAKIGKAAKQKAMGKFMKDRMVEDYEGVYRGNQAHR"
     gene            complement(5633..6601)
                     /locus_tag="VFD89_02830"
     CDS             complement(5633..6601)
                     /locus_tag="VFD89_02830"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HZJ57155.1"
                     /translation="MLISLTISSLILPIIILAARKFGITDKPDERKRHKQPKPLLGGL
                     GIFISFVITMLACVKHTIQIQHLIIYSGLMVLIGTIDDIKDVKAIYKLGIEIICATII
                     ALLGFRINLSHFLGLEGSGVITIDVVVSVIWIVGVTNAVNITDGLDGLAGGISFIAVV
                     AFSIIFMIFDYSLLNRVSFTLMGGILGFLIYNFYPSKLFMGDGGSLFMGFLLSLLSIM
                     YINADGSSASIFVPVVILAVPIFETWSSMLRRLLKGHSMMKADTDHLHDRLTEKGYSP
                     VITVSIIYFWAILAGILGIVIAVGNLPILALSIMVLMIAAAIKIYI"
     gene            complement(6616..7176)
                     /locus_tag="VFD89_02835"
     CDS             complement(6616..7176)
                     /locus_tag="VFD89_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57156.1"
                     /translation="MKTMIIQGLYKRTIMISLLIFILAICISFLVSNYVIEKTYESMG
                     ILLMYNGSPDEENRSLKDAKTYRAIATGTQMMNEITSELDIQYTVRDMRNKIIVSYED
                     NTDLLYIKTEDGDPEMAFKITKAMMDKLQNRVRELFGNSHLKVVEEPYIPFEPSGPNI
                     VLNMVIAGIFAIFLAVLVNVWNQKCR"
     gene            complement(7375..7449)
                     /locus_tag="VFD89_02840"
     tRNA            complement(7375..7449)
                     /locus_tag="VFD89_02840"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7415..7417),aa:Asn,seq:gtt)
BASE COUNT         2153 a         1799 c         1320 g         2183 t
ORIGIN      
        1 gctctgcaac actccagtaa ctatatagga aatactaata aatatgaagg ctggaaacat
       61 gatcctagca agtctagcgg ccataaccat attatatccc gataacctgg gggcaataac
      121 ggctattatc tgccttgaaa aagcggaccc cagccccgca aatatcaagc ccaccaaaaa
      181 agccatgttg attatattgc tggccagggc atggcttttc tcggggtttt tcttcaattg
      241 ctgcgtatat ataggtacaa agaccagacc catactggcc ccgattatgg agaacaatat
      301 agagggcagg ctgtatgcaa caaaataggc atccgttata ccacctgccc cgtaatatga
      361 ggctattaca gcctccctaa aaaaacctgt taactttcct gcgatggaga aaaccgttac
      421 aataaaaaca gaccttattg tcttatccct tgggcgcatg ggttaggcct ccctcctatg
      481 atgacgggta agaaagtact ttcttgttaa ctcctccatc gccaaccagc cttttatcct
      541 ggcaatgaac agataccttc cgtcgctcct caatacgggt atcctcttta aagcatgagt
      601 ttgttttatg ccttttttga ctattctctc cccattggat atgaggaccg ctgtgtcata
      661 atgttttctt aacaattgct ctccggcttt gctatagatc cccctagggt aagcaaaatg
      721 cctgacaggc ttttggagcc ccttttctaa cattgttttg gattcgacta tttccctttc
      781 caattcggcc ggagacacca ggttcatatc cctatgctct actgtatggc tcccaaattc
      841 cacaagacca tccccgtcca attcgtagat ttccttccac tccatcagct cagcatcagt
      901 ttcatcaagg tcccaccaaa atttctttgg gctctctata tatccgggac acaggtacat
      961 tatggagggg tatttgtacc tatggagtat tgggtatgcg tttttgtaga aatccctgta
     1021 tccatcatca aagcttataa caatatgtct ccctactatg ccattgtttg atatcataga
     1081 atatgctttt tccatggaaa tgacgctgta ccttttccta tacagatagc ccatatgcca
     1141 attaaagcta tctctggtca cagccaattc tttttttact ccatcatcca ccctatggta
     1201 catcaaaatt attatctcgt ctctggacct aaatagaacg gcgtgtataa tgcccaaggg
     1261 aagtaatagg tatttaatta tcgtctttat taaaaacaac ataggcttac tccgttatat
     1321 cttcaaattt gttgtaagac atcctcccaa accgcttttt aagtcttaag cgtttaaact
     1381 tactaaaacc tacatccttt gtaaggttca tgagataatc gctcctgcct cctgtcacca
     1441 aatagcaacg actataccct aatccattta aataggacaa gactgaattt aataacccgt
     1501 ggctgattct gctcctcatt cttccagtaa aatgctggta tataaaggtc gaaccccctt
     1561 ccatattcca attaaaatta agagcaggga tcttaatgct gtgggtatca atccaaaaac
     1621 tatatatgcc ttttttatcc cataccagaa tgcatctgtt ttcatctaaa aaccgcctat
     1681 gaacatcccg gggttccatc cccaattttt ctgtcagtag gcccaatttc cttatggatg
     1741 cctcctctac tgtatatatc ccgcagtccg ccttatcggg gcttataagc ggtctgtata
     1801 cgatgtaata atcataacta ctataaatat gtgcaacgat ctccacgacc ctgttcttca
     1861 atcgcccaat gccccgtcta aatgtctttt ttgcgttgcc ggaatagagc aacatcttga
     1921 atttacccaa tttgtatttt acgaaattat agatccgggg atcccttttt agaaattgtc
     1981 ttaaatttct cactaatctt tggatgttaa atattaagtt taccagtacg ccatcacctg
     2041 ggaatacaaa gccgatcata tcggctttct catctgtcca cgctgccttg tagggctcat
     2101 accctacacc aaaatcgaac accctataac ctttctcaaa acaatccttc agcttcttcc
     2161 ttaacaccag ctcacctgga ctaaatatcg caaaattatc atcatgtcct attcgataga
     2221 aggtatatct tccgttataa taaaagccat agataaagga tatgggagtt ccgtctacat
     2281 caatgatgtc cacgctagtt tcgaacggca ttttatcaga tagcgctaat tccttaaaaa
     2341 attcccttgt cctgcccttt gaaaatttgc tgtgcccgac ctttctttgc cacctttttt
     2401 catggatatc gaatatcctt tctatatccc catccctttc ggtaaagggt ttaaaggata
     2461 gctctcccag ccttgccaac cttttttctt ttcgctccat ggtacgtata ctgttgcgcc
     2521 cgaaccggcc ggctatatag ctgttataat ccctgttatg caaacctata aaatagcatt
     2581 ccaggctgga ttttaaatac ttccttcgcg tcttatccag atagtcttta aatatgttgt
     2641 attcgtcggc atctttaaat atcccatgga tattgaacat atgattgcct ttgagaccga
     2701 taaggtaatc caggatgcta cccaagactg cacatttgaa tccgcttttt atgggaaatc
     2761 ccatatagct gagttcattc cccccaataa accttatctc tttgtatccc ctagcaggca
     2821 cctccatcaa cggacaaaaa cccaatatat tattgtcatc cttagccaca aggaagaata
     2881 gtctttttcc ttccccatga tacttccacc acagataaag ccattcaaag gtcataaaaa
     2941 ctatatcagt attgttttct cttaagatac tgtcccacat cgctttgtac ggttttatct
     3001 tatcccagct cctgagcact tctatttcca gttttgctca cctccaacac accgatatct
     3061 aaaaaaaatg aactttagta attgttacgt ccgaacccca acatagcctc aggtataaat
     3121 ccaccactat ttgatagatg ggggtccaac gcttgtggaa tccggattat gcttatgatt
     3181 cattgtaatg gcggccatac caatacctat aaaaaaccaa aaatgtctcc aatgtatcgt
     3241 atctatgacg ataccattta ccatgattcc catcaaaata gcaaccagag aagccggcgt
     3301 cattacaata tacgttcttc cctgcaaatg gtgtataccc agttcgtata tgataaaggc
     3361 aagggagcca ataaataaga gaaggccgat tagcccattt tccatggcaa gtctgatata
     3421 gaggttatgt gcagaaaatt ctgccccgga atatcgtagc acattatatt cgaattgacc
     3481 cggtccgaat ccaaagatat ttatagaggc tagggcaatg ccctttctat gggttgcaaa
     3541 tcggtcctta tcatatgttt gaaagtttat cctgctaagt aaaaattgtt tgtattcatg
     3601 ggggattata aaacgccaaa ccacagatac aatcaccaat accagaaggg cgtagaggac
     3661 aagtctttta agttccgcag cggggagatg aatctttttt atattcaata caaaatatat
     3721 taacagacac gcacaggtat tcacccaggc cccccttgaa aaggagacga taatccctaa
     3781 cagatttata gctatatgaa taatgcggat ccaagacggg tgatcaaaga gttgtttttt
     3841 ccaagagtcc tcgattaaca tgacaacaac aggaaccaaa aagggtccaa aaacgttagg
     3901 gtccttaaaa agggccattg ccctgtacct gtcatacatc agcagcgaag gcaatacccc
     3961 cagataaccc gccaggccca acagggcgga gattgtgccc gagataatat atgcccttat
     4021 tatatttttg atattgtctc gggagacata caaaaacata taataactat aggctatcag
     4081 atacatagtg ataaggtgat accgtatacc cagcatcatt tcatgggaaa taaacagctg
     4141 taatgtactt atcaatatat agagtatggg aaataagatc atcggccttg tagggttcag
     4201 tcggtgagat tccaaacgat gggacataac tccgaccgca aaaagaagaa tgaacaccag
     4261 gtcagcgggt gccggttcga attttacaaa agataagatc agtatattca gtatcacagc
     4321 ccagtggtac ctgaagacca gcagggcacc gaaagcgcag atacctatta agacagatat
     4381 gtataccaga cccacacccg ttatgatggg ggataggccg gcgatcaggg cagctattgt
     4441 acataccgcc aaaatctttt tatcgatgag cctggtttcc cctgtatacc ccttcataat
     4501 cctctaccat cctgtctttc atgaattttc ccattgcctt ttgtttagcc gccttaccta
     4561 ttttcgcccg caaaccctta tcctggagca gaacagacag ttgatcggct ccatttttta
     4621 tatcgaagcc ctccaaaatc aggccgtcta tcccatgatc tataatttcc gataccccac
     4681 cgacaccgct gcagactaca gatttaccgg cgaacaaggc ttctatgata actataggca
     4741 acccttccca ctttgaaaac agtgttacaa tatccatatc gtttattagc tgccttgcgt
     4801 ctgcccgcgt ccctaacata aaaacgtgat cgctgattcc aaggctttct atggcctcga
     4861 cacatgaatc atgtaagggc ccatccccca caatgaccag tttggcccta tgccccatac
     4921 ttaaaatctc attaaacacc tgtatagtgt ataggggttg cttaggttcc cttaggcgca
     4981 taacagttcc gattatggcc acatccccat ggatattgag ttcatttcgt agtttaccaa
     5041 gcgtagtagg ggcctcgtct ataccgttat agataatagt ggatttttct cggctcaacc
     5101 accccatttt aagtcccaaa tccctatgat attttgaaac acatatgcac atggagcatc
     5161 ttttacttgc cagcttttca gcccacccaa ataatctttg gagccccttt ggttgatact
     5221 cgttgatgcc ccaaccgtgt acggtgaaat atattttagg gacaccggcc aaatgtgcag
     5281 ccagtcttcc caatatgccc atcttggagc tgtggaaatg ggctatatca tatttgccac
     5341 ccttcatgat cttgtatagc tttataagag tcaccagatc attgataggg gagatttccc
     5401 gcctaatttc gggaattcca attatcttta cgtctgcacc tctcaccgat cccaaatcct
     5461 taacccaatc aatgagttca ccccccggat aggtcaccag ggtcatatta tagaaatccc
     5521 ttggcagaga tgatataagg tcatatacta ccttttgggc tcctcccatc tccgaaaggg
     5581 ttatacccat taacaaattc acaggtttgt ctacacccat acctactccc ccctaaatat
     5641 aaatcttaat ggctgccgct atcatcagta ccataataga tagagccagg atcggtagat
     5701 tacccaccgc tatcactatg cctaaaatac cggcaagaat agcccaaaaa tatattatcg
     5761 ataccgtaat aacgggagaa tatcccttct ctgttaatct gtcgtgcagg tgatcggtgt
     5821 ccgccttcat catgctatgc cccttaagaa gccttctcaa catgctgctc cacgtttcga
     5881 atataggtac agccagtatt accaccggaa caaatattga ggcgctgctc ccgtctgcat
     5941 ttatatacat aattgacaat agtgataata aaaagcccat aaacaggctt ccgccgtcgc
     6001 ccataaagag tttggacgga taaaaattat atattaaaaa cccgaggatg ccgcccataa
     6061 gggtgaagga tacgcgattt aataggctat aatcaaagat cataaatatt atggagaaag
     6121 ccacaacggc aatgaatgaa atgccgcccg ccaacccatc caagccatcg gttatattta
     6181 ccgcgtttgt aacgccgaca atccatatga cgcttaccac cacatcaata gtaattacac
     6241 cagagccttc caaaccgagg aaatggctga gatttattct aaatcctaaa agggctatta
     6301 tggtggcaca gattatctct attcccaatt tataaatcgc tttgacatct ttaatgtcgt
     6361 ctatggtgcc aatcagcacc atcaatccgg agtatattat caggtgttga atttgtatgg
     6421 tgtgttttac acaggccagc atagttatta caaatgagat aaaaataccc agtcccccca
     6481 agaggggttt gggctgctta tgacgttttc tttcatccgg tttgtccgtt ataccgaatt
     6541 tccgtgccgc caaaataata atgggcagaa tcaatgatga gatagttaaa gaaattagca
     6601 tggataacac tgtaatcacc tacacttttg attccagaca ttaaccagga cggcaagaaa
     6661 tatagcgaat atacctgcaa tcaccatgtt taacactata tttggaccgg aaggttcgaa
     6721 ggggatgtat ggctcttcca ctaccttgag gtggctattt ccaaacaatt cccggaccct
     6781 gttttgcaac ttatccatca tggctttagt tatcttaaag gccatttccg gatccccatc
     6841 ctccgttttt atatataata aatcggtatt gtcttcatat gatacaatta ttttattgcg
     6901 catgtccctt actgtgtatt gtatgtccag ctcggatgtg atctcattca tcatttgggt
     6961 accggtcgct atagccctgt aggtctttgc atccttcagg gatctgtttt cttcatctgg
     7021 actgccatta tacattaaca gaatacccat agattcatac gtcttttcaa taacataatt
     7081 gcttactaaa aaactgatac atatagccaa gataaatatc aaaagggata tcattattgt
     7141 tcttttatag agaccctgta tgatcatcgt tttcattatt atcaccgccg catgtattta
     7201 tttgtagtag gctcagctat acatccatct tatgaaatgg acagcgcccg tgctacaggc
     7261 gtagaaaaat gatttgaggt cttatgacca gttgaatcac ggctgctata ttttctcggc
     7321 ggaataaaat aaaagcccct ttagcgattt gctaaagagg cctttatgta caattggctc
     7381 cccaggctgg attcgaacca gcaacccctc ggttaacagc cgagtgctcc accgttgagc
     7441 tactgaggaa tatta
//
LOCUS       DASRNX010000070         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_598534,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000070 DASRNX010000000
VERSION     DASRNX010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598534"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /gene="radC"
                     /locus_tag="VFD89_02845"
     CDS             <1..553
                     /gene="radC"
                     /locus_tag="VFD89_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZJ57157.1"
                     /translation="NDLAHRMIKEFGSLAGLFEADAKDIARRCKVSENTAVLVSLIPS
                     LSKRYFLAKWGDKPIINSSSKAGEYAVSLLVGQVYEVFYVVCLDTQNRVNYADMVHEG
                     TINEAAIYPRLIVETALRHQASSVILAHNHPGGSVNPSRADISVTKKIITALDSISIK
                     VLDHIIVAGDRYTSLAERGLLSN"
     gene            736..1209
                     /gene="msrA"
                     /locus_tag="VFD89_02850"
     CDS             736..1209
                     /gene="msrA"
                     /locus_tag="VFD89_02850"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZJ57158.1"
                     /translation="MKEIVLAGGCFWGVEEFMSRIPGVVDTKVGYANGTHAHPSYEDV
                     CTGATGHTEACYVKYNEDTIDLRELLDRFWSVIDPTIKDRQGNDIGSQYRTGIYYIDE
                     EDQDTITDSLNREQIKYTRPIVTEIEPLSCFYDAEEYHQRYLKKNPNGYCHIKLD"
     gene            1235..1537
                     /locus_tag="VFD89_02855"
     CDS             1235..1537
                     /locus_tag="VFD89_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57159.1"
                     /translation="MRTGCLGFLLSALLIIIIIVLLSGCVPGDGRNTLERPAGFFWGI
                     WHGWVAPISLILGILERDIRVYEVFNNGWTYDFGFYIAIISGFGGISLFRKKKGKK"
     gene            1551..2252
                     /locus_tag="VFD89_02860"
     CDS             1551..2252
                     /locus_tag="VFD89_02860"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013371575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZJ57160.1"
                     /translation="MLLNKVHYENIDIIYEDNHLLVAEKPVNMLSQQDISGDKDILTK
                     LKEYIKVKYNKPGNVYLGLVHRLDRPVGGVMVFAKTSKAASRLSKQVRDRGFGKRYMA
                     VVRGIPQIKSKEGLTHYLIKDRRRNMVATTQSGADGAQKAVLDYEVIGQASDMALVHI
                     DLHTGRSHQIRVQLAALGHPLYGDQRYGRGINKPGQQIALWATEISLVHPTLREAMTF
                     SSSPPHQYPWDMFGY"
BASE COUNT          713 a          465 c          625 g          577 t
ORIGIN      
        1 caatgactta gcccatagga tgattaaaga gtttggatca ctggcggggc tatttgaggc
       61 tgatgccaag gatatagcca gaagatgcaa ggtgtcggaa aataccgccg tcctggtttc
      121 cctaatcccg tccctgtcaa agaggtactt cttagctaaa tggggggata agcccataat
      181 aaacagctct tcaaaggcgg gggaatatgc cgtatcctta ttggtggggc aagtttatga
      241 ggtcttttat gtagtatgtt tggatacgca aaacagggta aattacgcgg atatggtcca
      301 tgaaggcacc atcaacgagg cggccatcta tccaaggctt atagtggaaa cagcccttag
      361 acaccaggcc agcagcgtta tactcgccca caatcatccg ggcgggagcg tcaatccctc
      421 acgggccgat atcagcgtta ccaaaaagat tattaccgcc ctcgatagta tttccataaa
      481 ggttttagac catattatag ttgcaggaga cagatatacc agtctggccg aaagaggcct
      541 tctgtcaaat tgattttgaa ttagggtgta tcaaaggccc ttaagtgcta aactatatac
      601 agcagcttgg tgcagataaa caatatggaa cctaaattta tgggcatgat aattacattg
      661 ggcctatgga atctcaaggt gatagatctt ataacatgag ttgtttatct gctgataagt
      721 cagggaggta ggattatgaa ggaaatagtg ctggcagggg gatgtttttg gggagtagag
      781 gagtttatgt cacggattcc gggggtagtg gataccaagg ttgggtacgc caacggaacc
      841 catgcccacc ccagctatga ggatgtgtgt acaggggcta ccggtcatac ggaggcatgt
      901 tacgtaaagt ataacgaaga tacaatagac cttagggaat tgctggacag attttggagc
      961 gtaattgacc ccaccataaa ggataggcag ggaaatgata tcggaagcca gtaccgcacc
     1021 ggtatctatt atatagacga agaggatcaa gacaccataa cagacagtct taacagggaa
     1081 cagataaagt atacaagacc catagttacg gagatagaac ctttgtcatg cttttatgat
     1141 gcggaggaat atcaccagag gtacctgaaa aagaacccca acggctattg tcatataaag
     1201 ctggactgat ggggattagg ggtgagagga tatgatgagg acgggatgcc ttggatttct
     1261 actttcggca cttcttataa taattatcat cgtattatta tctggatgtg tgcccgggga
     1321 cggacgaaat accctagaaa ggccggcagg attcttctgg ggaatttggc acggttgggt
     1381 ggccccaata tcattgatat tggggatatt ggagagggat atcagggtct acgaggtgtt
     1441 taacaacgga tggacctatg attttggatt ctatatcgcc atcataagcg gttttggcgg
     1501 tatatcgctg ttcagaaaga aaaaagggaa aaaataatca ttggtgaggt gtgttactta
     1561 ataaagtgca ttacgaaaac atagatatta tatacgagga taaccatctt ttagtcgccg
     1621 aaaaaccggt caatatgttg tcccaacagg atatatccgg ggacaaggat atattgacca
     1681 aattgaagga atatataaag gtgaagtaca acaagcccgg aaatgtgtat ctgggtttgg
     1741 tgcatagatt ggaccgtcct gtaggcggtg taatggtatt tgccaagacc tccaaggccg
     1801 cttccaggct ttctaagcag gtacgggaca ggggatttgg caaaaggtat atggcggtgg
     1861 tccgcgggat accccaaatt aaatcaaagg agggcctaac ccattatctt ataaaggaca
     1921 gacgaaggaa tatggtggcc acgacccaaa gcggcgctga tggggcacaa aaggcagtat
     1981 tggactatga ggtcataggc caggcatccg atatggccct ggttcatata gaccttcata
     2041 ccgggcgctc ccatcagata cgggtgcaat tagctgccct agggcacccc ctgtatgggg
     2101 atcagcggta tggaaggggc attaataagc cgggtcaaca gattgccctt tgggctacag
     2161 aaataagcct ggtgcatcct accctaaggg aggccatgac cttttccagt tcgcccccgc
     2221 accaatatcc ctgggatatg ttcggatatt agttaaataa gaaccgataa tagggcatca
     2281 taaaacatca gaaagcattg gggaacattg gggatatcac aaaaggccca tggaatctca
     2341 taaagcactt gaaacacctg aaacatttga aaccccatac
//
LOCUS       DASRNX010000071        15656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_651237,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000071 DASRNX010000000
VERSION     DASRNX010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15656
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651237"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..635
                     /locus_tag="VFD89_02865"
     CDS             219..635
                     /locus_tag="VFD89_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZJ57161.1"
                     /translation="MVVLIALFAMICWGIAPIFGKLGLSDLDPLVGLAYRTYLSTALL
                     TGWMIRDNTFSKMAGVSLKSIIFLGIEGILATLVGDLAYYAAIKYGEISIVTLIMSCS
                     PIISIIMAILIFDEQLTPVKFVGAGLIVIGLTLIVR"
     gene            complement(663..2138)
                     /locus_tag="VFD89_02870"
     CDS             complement(663..2138)
                     /locus_tag="VFD89_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZJ57162.1"
                     /translation="MGRKWNLDQTSAPLFEAIRRHIESGVVPFHVPGHKGGRGIIEEF
                     KEYVGEQVFRMDLNGMDDLDNICNPLGAIKESEDLMAELFGAGDSFFLVNGTSQGVQA
                     MILYACRPGEKIIVPRNVHKSVIGGLILSGAIPIYIQPEFHARLGIALGVEPQSLRAC
                     INRHPDARAVFINNPTYYGVASDIKAIIGIAHENDMLVLADEAHGAHLPFHPDLPPSA
                     ISQGGDLSAISLHKTGGSMTQSSVLLSNNKNINPAAMKTVINLMQTTSASYILMASLD
                     LARKHLALRGDHMMERAVSLAKYARQGVNKIDGLYAFGTELVGHPGIFGFDETKLCIN
                     VRELGMSGYEMESFMRKQNKIQMEMSDFHNTLAVISLGDDRESVDRLIDGLGDIARNR
                     KGGGFDAPMVIPPALEMVVSPREAFYLQKKTVPLESSAGEVSGEMVMAYPPGIPALSL
                     GELITKDIIEYIKMMKKSKYQLQGGEDPFINNINVLSRERV"
     gene            2375..3712
                     /locus_tag="VFD89_02875"
     CDS             2375..3712
                     /locus_tag="VFD89_02875"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZJ57163.1"
                     /translation="MLEGRNRYGCIVFKGGEWKLKDIVIENLDKNTNLYVIPDKKFKT
                     IYISYCFHRDLDENYTYNALVPAVLKRGCEGYENQKEIGKHLEEQYGALFDVGVQKRG
                     ERQILRFTMDVINEDYVGKDGLLAQAFYFLNRIITKPVLEDSMFREDYVDQEKENLKN
                     RILAMINDKMQYSMERCIQEMCQGEKYARYALGSVDELSSLDPEKLYEVYRDIITGSP
                     LDIFVVGDVDPGKVSALIKNSLDLERSHIKLIPETIIKKIGIKQREVVERMDVSQAKL
                     NIGFRTNVPVNQDEYFPLVLYAGILGGGPHSKLFINVREKSSLAYYAFARLEKYKGLM
                     LIGSGIDQKDYEQALEIIMEQIADIEKGRISDMEFTASVNAITTSLRAAKDEQGQLVD
                     YYLGNAITGARHTLEDFIREIQKVKPEAVVEVSKKVKADTVYLLRPNEGDVEN"
     gene            3709..5001
                     /locus_tag="VFD89_02880"
     CDS             3709..5001
                     /locus_tag="VFD89_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZJ57164.1"
                     /translation="MKKVIENKLLREKLVHSRHRSGLDVYIMLKPGYSRQFAIYATQY
                     GSNDSQFVPRGRRDSITVPDGIAHFLEHKLFEEEEGNVFAEYSKLGAQPNAFTNFNMT
                     AYHFTSTGNFHECLDVLLGFVGRPYFTDENVEKEKGIIAQEIRMYEDNPYWGVYFNLL
                     RGLYHDFPVRKDIAGTVESIYQIDKETLYECYKTFYHPSNMALIVAGDVDPDRVIRQV
                     DELFADGPTGNGAGEIKRIFPEEAKGVNKNRVEEKLAVARPIFALGFKDEEDVQDGDR
                     LLDKLIANDIILDMLMGTSSDIYDELYEEGLIDSSFSSSYTGERHYGYALMTGQTKDP
                     EKVVAAIFDEIEKRKSQPWEQEHFTRIKKKKMGEYIRLFNSLDSTSIQFVSMLFKDVR
                     LLDYLERLEAITYNEIKKEFNELYSKDSSCLSSIMPSA"
     gene            5055..5840
                     /gene="lgt"
                     /locus_tag="VFD89_02885"
     CDS             5055..5840
                     /gene="lgt"
                     /locus_tag="VFD89_02885"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZJ57165.1"
                     /translation="MSGVAFYLFGKPIFWYGIIIALGVLIGIYLAMRYAKALGYDQEM
                     VVDFCLLAIPMGIIGARLYYVVFSWEYYSRNPLDAFKIWEGGLAIYGVVIGGITSAYI
                     FGRKRKINFWDLCDIIAPSLILGQALGRWGNFFNQEAYGYAINNPAWQWFPAAVYINS
                     TGQWHMATFFYESMWNFLVFFFLLFYKKRRKIPGEMILLYLILYSLGRVFIEGLRTDS
                     LYWGQFRVSQLLSGALIIIGIAAFFIRRKRASNDDEGIREEGD"
     gene            6097..6483
                     /locus_tag="VFD89_02890"
     CDS             6097..6483
                     /locus_tag="VFD89_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZJ57166.1"
                     /translation="MADDNVRRDEDRQQEGKGRGWLGIIIRFIVAAVVLMVTAVVTPG
                     FRAMGFGTALLAALIIAALDYVVQKLFRIDASPFGRGLSGFILSAVIIYLSQFLVPGM
                     QINLFGAVIAALIIGVVDALIPAHIF"
     gene            6826..7437
                     /locus_tag="VFD89_02895"
     CDS             6826..7437
                     /locus_tag="VFD89_02895"
                     /inference="COORDINATES: protein motif:HMM:NF020450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HZJ57167.1"
                     /translation="MTIESFMVRDDIEELIEEIKAYDSIRLDEIPEYRLFISQIEEFF
                     DKKLGRGMDDDEERKTISKTMIQNYIKDGLIMPPEGKSYNRSHIILLALIYNLKPILT
                     IRDIKKLLLPILEIADDESQTNKIESIYNTYFDLKESHLDEFVQNLECDMNAMDEILD
                     KECNGEWIRLSKILLVLMLITQANIRKRLAERMIELYFNTEES"
     gene            7678..8004
                     /locus_tag="VFD89_02900"
     CDS             7678..8004
                     /locus_tag="VFD89_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57168.1"
                     /translation="MKKRSMGPKGDGRQRAKNHKKNYLHGNYIYVTDHAITRYRQRIK
                     KINHENAKYSIIENVKRSRLIALTKYGGREIRENRGVLYVCELQGNCLYVITILIGDV
                     DLRFVV"
     gene            8259..9695
                     /locus_tag="VFD89_02905"
     CDS             8259..9695
                     /locus_tag="VFD89_02905"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HZJ57169.1"
                     /translation="MRNLTMLTDLYQLTMMYAYDKAGKADEVVAFDMFFRKPTGDSAY
                     AVAAGLEQAVEYIENLNFSDDDIAYLRDLGLFDESFLNRLEHISFTGDIDAIPEGTVV
                     FPNVPLIQVRAPIFEAQFIETALLNIVNHQTLIATKASRVVQAAQGDSVLEFGLRRAQ
                     GPDAGIYGARAAIIGGCDATSNVLAGKKFGVPIQGTHAHSWIMSFPTELDAFRAYAHA
                     FPNSCLLLVDTYDTLKIGVPNAITVFKELREKGFEPLGIRLDSGDLAYLSKEARRMLN
                     DAGFPNAKIVASSDLDEDVIWDLKAQGAAVDVWGVGTAMITSKNCPALGGVYKLVAEE
                     IDGELVPKIKISENAFKITNPGYKKVSRLYDKETHKAIADLIMLDHEIIDETKPLTIF
                     DPVNTWKRMTLTNFKARELLVPIFRGGKRVYELPPLMDIQKYAKKEMGTLWAEYKRLT
                     KPHVFKVDLSDELYNLKNQLLEEYSFKQ"
     gene            10003..10890
                     /locus_tag="VFD89_02910"
     CDS             10003..10890
                     /locus_tag="VFD89_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZJ57170.1"
                     /translation="MRFGLALSGGGIRGVAHIGVLRALEEEGMMPSWIAGTSAGSIVA
                     GLYAYGYSPRELEEIAIDLQPKYIDPNFLGMALGLGQWVLGKEPCVDGVIKGKAIERY
                     LKKLTEGIKIADIKMPLAITAVDINKADSVIFLNRRVGILPDDDTVYMHDMLLYKAIR
                     ASIAIPVVFKPIILEGRRLVDGGVTNNLPIGILRQMGAERIIGVDLGYNGQLRRDVNN
                     IIEIGSQALDIMAYQITSFKSQGADYILRPGIYDIAIGDSKKLIECIDRGYNSTKENI
                     KMIKKAIISSPSTSVFYRI"
     gene            11131..11628
                     /locus_tag="VFD89_02915"
     CDS             11131..11628
                     /locus_tag="VFD89_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZJ57171.1"
                     /translation="MKKKFRVSLFGYCKKQVNMYMLNLRKEYEDELSKRKDRMWEVNA
                     ENRELKAKLLEYQESLSRYNEQELYISKAMVKAEENAQAILKESHHKIGIEKFKMNQE
                     KEKWKTREKEIMNQLLDFQNEAYALMESFQSEISYLTSKELMSSELEVDDSRTDINKD
                     LLNIS"
     gene            11917..13341
                     /locus_tag="VFD89_02920"
     CDS             11917..13341
                     /locus_tag="VFD89_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZJ57172.1"
                     /translation="MAKPNILFIMSDDHASQAISSYSGRINQTPNIDRIANEGTRFDN
                     CFCTNSICAPSRAVILTGKYNHLNGVMTLFDDFDGRQDHVAKLLQDTGYQTSIIGKWH
                     LGHGGNSDPTGFDYWCVLPGQGEYHNPVFYDMGEEIKVEGYATDIITDMSIDWMDKRD
                     KDQPFFLMCHHKAPHRPWEPSEKYMDLYEDIDIPEPDTLFDDYDTRSDAASLAKMRIS
                     HHLTKTDVKQDPPEGLEGVELTRWYYQRYIKDYLRCIASIDDSVGRLLDYLDEEGIAD
                     NTIVIYTSDQGFFLGEHGWYDKRFMYEESLRMPFLVRYPKAIKPGTINEDMIVNTDFA
                     PTFLDYAGLDVPKDMQGYSLRPIFEGNTPNHWRQSMYYRYWMAQDDHCVAAHYGIRTH
                     KYKLICYYAPGFHYNGEYPKELEGVEWPQWELFDLERDGDELNNVYDDPEYSGVVEEL
                     KEKLYSLQMELKDDKDARYEKREI"
     gene            13871..14467
                     /locus_tag="VFD89_02925"
     CDS             13871..14467
                     /locus_tag="VFD89_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZJ57173.1"
                     /translation="MDKRVNMKLLTDIFITFFKIGSFTFGGGYAMIPLIEREVITNKG
                     WIEDEEEIIDVFAVSESIPGAIAINSSTFVGYKIAGRKGAIAAMFGVVLPSFIIITLI
                     AAFFSKFQDNEVVKATFMGIRSAVVALILMAAINVGKKAIKDKFSAAISATTVILVLV
                     ADIHAIFAIIGGVLAGLIIYFTSPKRLAHIKGKGGEDN"
     gene            14464..15048
                     /locus_tag="VFD89_02930"
     CDS             14464..15048
                     /locus_tag="VFD89_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZJ57174.1"
                     /translation="MIYLRLLLTFVKIGFFSFGGGYAMIPMIEREIEGHGWLNALEFF
                     DIVAIAEMTPGPIAVNCATFVGYKTAGFLGGFAATLGVAIPSLVLILIISRYFFKFQD
                     HPLNAAVFYGIRPVIAGLIATAAVFVSETAIFKGKLSINLISRLLRSPLDVLNLGSIF
                     IFVISFIILKKTKFHPMLVISLSGALGILFFYVL"
     gene            15131..>15656
                     /locus_tag="VFD89_02935"
     CDS             15131..>15656
                     /locus_tag="VFD89_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZJ57175.1"
                     /translation="MPPVSLLIKPASSNCNLRCKYCFYHSIAENRDVKSYGIMDLDTL
                     ETLVKKTLEFADVTCTFAFQGGEPTLAGLEFFEKLIEFQKKYNKKDVQINNAIQTNGM
                     VIDERWAKYLHDNNFLVGLSLDGTKDVHDMGRVDPRNRGTFNNVMKTAGLFNKYNVEY
                     NMLFVVSSGVARHIT"
BASE COUNT         4688 a         2946 c         3710 g         4312 t
ORIGIN      
        1 ttatctacca tgttttaagg gaattgaatc aaaagaattt tgatacccta aaaagaggta
       61 tcgatcatca agctcaatgg ctttatttta ccacatgggt ggagatattc aaaatcataa
      121 tatgctgttg tccctgcata agaattagat atcattaaaa catcggaagc gattgtccga
      181 tggtacctat gtgtcacttt gaaggggggc gcattggctt ggttgtttta attgcgctgt
      241 ttgccatgat ctgctgggga atagccccga tattcggtaa actaggtctt tccgacttag
      301 atccgttggt aggccttgca tacagaacgt acctgtccac ggcattatta acgggttgga
      361 tgatcagaga caataccttc tcgaagatgg caggcgtatc tttaaaatcc attatatttt
      421 taggtataga ggggatattg gccaccctag ttggggacct ggcgtattat gcagctatta
      481 aatatggtga aatttccata gttacactaa tcatgtcctg ttcccccata ataagcatta
      541 taatggctat attgatattt gatgagcagc taaccccggt aaaatttgtc ggtgccggtc
      601 ttatagtcat aggcttgacc ctcatagtga gatgaggaca aaagcgtagg ggattctcgc
      661 atctacaccc tctcacggct taaaacattt atattattta taaaggggtc ttcgccaccc
      721 tgaagttgat acttggattt tttcatcatt tttatatatt ctattatatc tttggttata
      781 agctcaccta ggcttaaagc cggtattccg ggtgggtatg ccataaccat ttccccgctg
      841 acttctccgg cggaactctc taggggtacg gtcttcttct gtaaataaaa ggcctcccgg
      901 ggtgaaacca ccatctccaa agccggcggg ataaccatag gcgcatcaaa accgccccct
      961 ttccgattcc tcgcgatgtc cccaagcccg tctataagac gatctacaga ttctctatcg
     1021 tcacccaggc ttattactgc caaggtatta tggaaatcgg acatttccat ctgaattttg
     1081 ttttgctttc tcatgaacga ttccatttca tatcctgaca tccccagttc acggacgttt
     1141 atgcatagct tggtctcatc aaagccaaag atccccggat gacccacgag ctccgtacca
     1201 aaggcgtata gaccatcaat cttgttgact ccttgccttg catattttgc tagggatact
     1261 gccctctcca tcatatgatc gccccttaag gccaaatgct tacgggccaa atccagggac
     1321 gccataagaa tataggaggc gctggtggtc tgcatgagat tgataacagt tttcatcgca
     1381 gcagggttga tattcttatt gttagataag agcaccgagc tttgggtcat cgagccgccg
     1441 gttttatgga gacttatggc gctcaagtcc cctccttggg atatagccga cggaggcaga
     1501 tccgggtgaa agggcagatg agctccatgg gcctcgtctg caaggaccaa catgtcgttt
     1561 tcatgggcga tgcctattat agctttgata tcagaggcta caccgtagta ggtaggatta
     1621 tttataaata ccgccctagc atcgggatga cgattgatac aggccctcag gctctggggc
     1681 tcaacaccta aagcgatccc cagcctggca tgaaattcgg gctgtatgta tatgggtatg
     1741 gcgccgctca gtataaggcc ccctataacg gatttatgca cgtttcttgg gacgattatt
     1801 ttctcacctg gccggcaggc atatagtatc atggcttgca ccccctggct agttccattg
     1861 accaggaaga aggaatcccc cgctccgaaa agctcggcca ttaaatcctc ggattccttt
     1921 attgccccta ggggattaca tatattgtca agatcatcca taccatttaa atccatacga
     1981 aatacttgtt caccgacgta ttccttaaac tcctctatga tcccccttcc ccctttgtgg
     2041 cccggaacat ggaagggaac taccccgctt tctatatgtc ttcttatggc ctcaaataat
     2101 ggtgcacttg tctggtctaa attccatttt ctgcccatga aacgaagtcc cccttactct
     2161 accaatcaat cacagtatat gcgctgtagt atagtttgtg attaaggcct acggggagca
     2221 caagaattgg atagaaaact attttcataa caataatata gataaacaat tccaactaca
     2281 gaatttatca tgattaaatt gcgtagaccc gtcggagcta ttatgatgtt agatttagtt
     2341 ttgtattgtt tatctgtaga ttagaaggac ttttatgctg gaagggagaa atagatatgg
     2401 atgtatcgta tttaagggag gcgaatggaa attgaaggat attgtaattg agaatctgga
     2461 taagaacacg aatctatatg ttattccaga caagaaattt aaaactatct atataagcta
     2521 ttgtttccat agggacttgg atgaaaatta tacctacaat gccttggtac ccgccgtatt
     2581 aaagcgtggg tgtgagggat acgagaacca gaaagagata ggaaagcacc tggaagaaca
     2641 atacggtgcc ttgtttgatg tgggggtcca gaaacggggc gagcgccaga tattgaggtt
     2701 cactatggat gtaatcaacg aagattacgt aggaaaggat ggcctattgg cccaggcctt
     2761 ttatttccta aacaggataa tcacaaaacc cgttttagaa gactccatgt ttagggaaga
     2821 ttacgtcgat caagagaaag agaatctgaa aaacaggatc ctagctatga tcaacgataa
     2881 gatgcagtac agtatggaac gctgtattca ggagatgtgc cagggtgaaa aatatgcaag
     2941 gtatgccctg ggaagtgttg atgaacttag ctccttggat ccggaaaaat tgtatgaggt
     3001 ttatagggac attataactg gcagcccctt ggatattttt gttgttggtg atgtggaccc
     3061 cggcaaggtt tctgccctta ttaagaacag tttagacctt gaaagatcgc atattaaact
     3121 cattccggag actataataa aaaaaatcgg gataaagcaa agggaagtcg tagagaggat
     3181 ggatgtatcc caagcaaagc tgaacatcgg atttaggacc aatgttcccg ttaaccagga
     3241 tgaatacttc ccactggtgc tatatgccgg aattttgggc gggggacccc attcgaagct
     3301 ctttattaat gtccgagaaa agagtagcct tgcctactat gcctttgcta ggctggaaaa
     3361 gtataaaggt ttgatgctca taggctcggg tatagaccaa aaggattatg agcaggcgct
     3421 agagatcatt atggaacaga tagcagacat tgaaaagggt aggataagcg atatggagtt
     3481 tactgcctcc gttaatgcaa taactacctc tttaagggcc gcaaaggatg aacagggaca
     3541 actggtggac tattatctgg gtaacgctat tacgggggcc aggcatacat tggaggattt
     3601 tatccgagaa atacaaaaag taaaaccgga ggctgtcgtt gaggtatcta aaaaggtaaa
     3661 ggcagatacg gtctacctcc taaggcccaa tgaaggagat gttgagaatt gaaaaaggtt
     3721 attgaaaaca aactgttgag agagaaactg gtacatagca ggcataggtc cgggcttgac
     3781 gtatatataa tgttaaaacc cggatatagt cggcagtttg ccatatatgc aacccaatat
     3841 ggctccaatg acagtcaatt tgttccccgt gggcggcggg attccattac ggttccggac
     3901 gggattgcac attttttaga gcataagctg tttgaggaag aggaaggcaa cgtatttgcg
     3961 gaatattcta aactgggtgc ccaacccaac gcctttacga atttcaatat gactgcatat
     4021 cattttactt ccacgggaaa cttccatgaa tgcctagacg tactccttgg ttttgtagga
     4081 agaccctatt ttaccgacga gaacgtcgag aaggagaagg ggataatagc gcaggagata
     4141 cggatgtacg aggacaatcc ctattggggg gtgtatttta acctgctaag gggtctgtat
     4201 catgattttc cggtaagaaa ggatattgcg ggaaccgtag aatccatata tcaaattgac
     4261 aaggaaaccc tttatgaatg ctataagacc ttctaccatc cgtccaatat ggcattgatt
     4321 gtggccgggg atgtagaccc cgacagggtt atccgtcagg tagatgaact ctttgctgat
     4381 gggcccaccg ggaacggtgc gggagagatt aaaaggatat tccccgaaga ggccaagggg
     4441 gtcaacaaga atagggtaga agaaaaactg gcagtagcca ggcccatatt tgccctgggc
     4501 tttaaggatg aagaggacgt tcaagacggt gacaggctgt tggataaatt gatagccaat
     4561 gatatcatcc tggatatgct aatgggtacg agttccgata tctatgacga actctacgag
     4621 gagggactca ttgattcctc atttagtagc agctatacag gcgagagaca ctatgggtat
     4681 gccttaatga cggggcagac caaggatccg gaaaaggtag ttgctgccat atttgatgag
     4741 atagaaaaaa gaaaatcaca accttgggag caggagcatt ttaccaggat caaaaagaaa
     4801 aagatgggcg aatatataag gttgttcaat tccctggatt ctaccagcat acagttcgta
     4861 tccatgttat ttaaggatgt tcgcctattg gattatcttg aaagattgga agccataact
     4921 tacaatgaga taaaaaaaga attcaatgag ctctacagca aagacagtag ctgcctttcc
     4981 tcgataatgc cgtctgcata ggatgtttta gcttaccggt agcctgtaat catattatac
     5041 gagggggaat ataaatgagc ggtgtagcgt tttatctatt tgggaaacca attttctggt
     5101 atgggataat tattgctctg ggggtattga taggtattta tttggccatg aggtatgcaa
     5161 aggcattggg atacgatcag gaaatggtag tggatttctg cttattggct atacccatgg
     5221 ggattatagg tgcacgcctc tattatgtgg tgttttcatg ggaatattac agcaggaacc
     5281 ccttagatgc gtttaaaatc tgggaagggg gcctggcgat ctacggagtt gttatcgggg
     5341 gaataacaag tgcctatatc tttggccgaa aaagaaagat caacttttgg gacctgtgcg
     5401 atatcatagc gcccagtctg atattgggac aggccttggg aaggtggggc aacttcttta
     5461 atcaagaagc ctacgggtat gccataaata accccgcatg gcaatggttc cccgctgctg
     5521 tatacatcaa cagcacaggg cagtggcata tggcaacatt tttttatgaa tccatgtgga
     5581 actttctggt cttcttcttt ctgttattct ataaaaagag gaggaagata ccgggagaga
     5641 tgatcctact gtaccttata ctatactctt tgggccgggt atttatcgag gggctgcgaa
     5701 ccgatagtct gtactgggga caattcagag tctcccaact attgagtggg gcactgatca
     5761 taataggcat tgcagcattt ttcataagaa gaaaaagagc atctaatgac gacgagggga
     5821 tacgggaaga gggggattaa cccctcttct ttaagttttg ggccgatttt ttaaaataat
     5881 tcattaaaag attatatttg ggaaaaatat atccagtgga tactctagga tgcgtatagg
     5941 atttaattgt gaattttcgg gaaaccatgg ctaatacggt tttggaagaa attccattaa
     6001 ttaaattccc gattttggtt caatatatga gtataccgat taatgtgata ctagttattt
     6061 gaaggcaatg agacaagaag gaaaggagca tataaaatgg ctgatgataa tgtacgtaga
     6121 gacgaagatc gccagcaaga aggcaaaggc aggggttggc tgggtataat tattcgattt
     6181 atagtagcag ccgtggtgct aatggtgact gcggtcgtta ctccgggatt tagagcaatg
     6241 ggattcggga ctgcgttgct ggctgcgttg ataatagcgg cacttgatta tgtggttcaa
     6301 aaactgttca ggattgatgc atcacctttc ggaaggggat tatcagggtt tattctgtcg
     6361 gcggtaataa tttatttaag tcagttttta gtcccgggta tgcagataaa cctattcggt
     6421 gcagtaatag ccgcgttgat cattggggta gttgatgcct taatccctgc ccacatattc
     6481 tgactagact ttagctgggg cggaggactt ccccattggc tataccagta attaatgttc
     6541 ggacataagc ctcaggcgtg ttttaaggaa ttattatgag ccctccttat agcacgccct
     6601 ttgttttgga ttttccgcta tactattgtt ttggatttcc tgctatactt tttttgggtg
     6661 tatcatttat ttaaaaaata atattccaat cacttggatt ctaggggcta aacaattcct
     6721 cggattcgga ttatgccccc gcatttttcg gttgcttgaa aaaaagccgg tgatactgta
     6781 taattggggc gacaagaacc tattgtatga taaagaggtg caagcatgac cattgaatcc
     6841 tttatggtta gagatgacat tgaagaatta atcgaggaaa taaaagcata tgatagtatc
     6901 cgcctggatg agataccgga ataccgatta ttcatttctc agattgaaga gttcttcgat
     6961 aaaaaactgg gcaggggtat ggacgatgac gaagaacgga agaccatctc taagaccatg
     7021 atccagaatt atattaaaga tgggcttatc atgcctccgg aaggcaaatc gtataaccgt
     7081 agccatataa tattattggc attaatatat aatttaaagc ctatactcac cattagggac
     7141 ataaaaaagc tcctgttgcc gattctggag atagcggatg atgagagcca aacgaacaaa
     7201 attgaaagta tttacaatac atattttgat ctaaaagaat cccatctgga tgaatttgtt
     7261 caaaatctag agtgtgatat gaacgccatg gatgaaattc tggataagga atgtaatggg
     7321 gagtggataa gactctctaa aattctgctg gttttaatgt tgataaccca agcaaatata
     7381 aggaagagat tggccgaacg catgatagaa ctatatttta acaccgaaga aagttaaccc
     7441 ccgcggacta ccgttgaatt ccatatgccc caagaacccc cctattaatt aaaattgcga
     7501 acccatagat ccttaaatga tgattccatt gtccctttta tgaaattcgc cgggatgctc
     7561 gtataaagtt tttgaaccat tgaaaaacag ttatgaatac tatataatag agttaatata
     7621 tcggcgtacc ctatgggcaa aaactcccaa cggggattag cataaggggc aggttggatg
     7681 aaaaaaagga gcatgggacc aaagggagac ggtaggcaga gagcgaagaa tcataagaaa
     7741 aactatctac atgggaacta catatacgta acggatcatg caataactcg ttatcggcag
     7801 aggataaaaa agatcaacca tgaaaatgct aagtattcca ttatagagaa tgtgaagagg
     7861 agcagactca tagccctaac caaatacggt gggagagaaa ttcgggaaaa ccgaggggtg
     7921 ctctacgtat gcgaacttca gggaaactgt ctgtacgtaa taaccatcct aatcggtgat
     7981 gtagacctaa ggtttgtagt ataaagtgat aaattagaga aaaataggga caatactagg
     8041 tctaaacctg atttgtccct attttttgtg tccttttatg aaaatctatg gcattatccc
     8101 gtattaggta gtataataat tatcgataaa caatacgcaa acttaattta tctgcatgac
     8161 aatttcagtg attttatgga atttcatgat gataaatttt atagttggag ttgtttatcg
     8221 acaacaatac gcaaataaat ttactagagg cgataaacgt gagaaattta acaatgctga
     8281 cagatcttta tcaattgacc atgatgtatg cctacgacaa ggcgggcaaa gccgatgagg
     8341 ttgtggcctt tgatatgttt tttagaaaac cgacaggcga tagtgcctat gccgttgctg
     8401 caggcttgga acaggctgta gaatacatag aaaatcttaa tttttctgac gacgacatag
     8461 cgtatcttcg ggatttagga ctgtttgatg agagcttttt aaacagactg gaacatataa
     8521 gctttaccgg tgatatcgat gctataccgg agggaaccgt agtatttcca aatgtgccct
     8581 taattcaggt tagagcacca atatttgagg cacagtttat agaaactgcc ctcttaaata
     8641 ttgtaaatca ccagacttta attgccacaa aggccagccg agttgtccag gctgcccaag
     8701 gcgatagcgt attggaattt ggattaaggc gtgcacaggg accggatgcg ggtatatacg
     8761 gtgccagagc cgccataatt gggggttgtg atgccacctc caatgtattg gccggtaaaa
     8821 aattcggtgt ccccatccaa ggtacccacg ctcatagctg gataatgagc ttcccgacgg
     8881 aattggatgc ctttagagca tatgcccatg cgtttcccaa tagctgcctt ctgctggttg
     8941 acacctacga cacgttaaaa atcggtgtac ccaatgctat aacggtgttt aaggaattga
     9001 gggaaaaggg gtttgaaccg ctggggatcc gcctagattc gggggatttg gcctatttaa
     9061 gcaaggaagc taggagaatg ctcaacgatg cgggttttcc caatgctaaa atagtagcct
     9121 ccagtgacct ggacgaggat gtcatatggg atctgaaagc ccagggtgcc gctgttgatg
     9181 tttggggcgt agggactgca atgataacca gtaaaaattg tccagcctta ggtggggtat
     9241 ataagctggt ggccgaggag atagacggtg aactggttcc aaaaattaag atctctgaga
     9301 acgcctttaa gataaccaac cccgggtata aaaaggtctc ccgtctatat gataaggaaa
     9361 cgcacaaggc aatcgctgac ttaataatgc tggatcatga aattatagat gagaccaagc
     9421 ccttgactat tttcgatcct gtaaatacat ggaagcgaat gaccctaaca aactttaaag
     9481 ctagggagct attagtacct atatttaggg gaggaaagcg ggtttatgaa ctgccgcctc
     9541 ttatggacat ccagaagtac gcaaagaaag agatgggcac cttgtgggca gagtataaac
     9601 gcctgacaaa accccatgta tttaaggttg atctatccga tgaactatat aacctaaaga
     9661 atcagctttt agaggaatac tcttttaaac aataattagt tcgagacccc ctgcagattt
     9721 agcgcggggg gttttaattt taacggactc ttatcactcc ccatttaaaa ccgtcataac
     9781 cgataatcac ctcctgatat cgtcgcacat actatgcata catagacaca tggttaaagc
     9841 tttaaatcac ctaaaggaaa ccactttttg catttataat atacgaccct taggaggcgg
     9901 aatccatgtt cccctctatt tcatcaaatt ttaatgaatc ggcaagaaat cccttgcttc
     9961 tataagtggc tagttcccat tagtaaaaca ggcggtggta aaatgaggtt tggtctggca
    10021 ttatcaggtg gcggcattag gggcgttgcc catataggcg ttttgagggc cctagaagaa
    10081 gagggcatga tgccctcttg gatagccggt accagcgccg gtagtatagt agcggggctc
    10141 tatgcttatg gttattctcc cagggagttg gaggagatag ccattgatct tcagcctaaa
    10201 tatatagatc cgaacttttt gggtatggct ttaggattag gtcaatgggt cctaggcaag
    10261 gagccttgtg ttgacggtgt gatcaagggt aaggctatag agaggtattt aaaaaagttg
    10321 acagagggca ttaaaatagc ggatataaaa atgccgctgg ctataacggc cgtagacata
    10381 aacaaggctg actccgttat ttttctcaac aggcgggtag gcatcttgcc ggatgatgac
    10441 actgtttata tgcatgatat gctcctctac aaggcaatca gggccagcat cgctattccg
    10501 gtggttttca agcccattat tctagagggg aggcgtctcg tcgatggggg tgtaaccaat
    10561 aatcttccta tcggcatcct ccgacagatg ggggcagaaa ggataatagg cgtggattta
    10621 gggtataacg gtcagctgcg aagggatgtt aacaatataa tcgagattgg cagccaggct
    10681 ctggatatca tggcgtatca gattacttcc tttaaaagcc agggtgctga ttatatatta
    10741 agacctggaa tttatgatat tgccatcggg gattccaaaa aactaattga atgtattgat
    10801 aggggataca atagcaccaa agagaatatt aaaatgataa aaaaagcaat tattagtagc
    10861 ccttcgacat cggtattcta cagaatctag gcttacacca atgatatact atacacgggt
    10921 gtatagccac atggatatcc tttataaagt gaatccgact aactccatat ccaaacgtag
    10981 gatcctgctg aatcgattag gcttccaatt tcacggttag ggttcagttg tatcttttct
    11041 tgggtgagtt caatatatgc ccgtaatacg gtctgaattg agtatggaag taagcgcata
    11101 ttactaattc cgggaggtgc tggtataccg atgaagaaaa agtttagagt atccttattt
    11161 ggatactgta aaaagcaagt aaacatgtac atgctaaacc taaggaagga atacgaagac
    11221 gagctctcca agcgaaagga tagaatgtgg gaagtgaacg cggagaatag ggagctgaaa
    11281 gccaagttat tggagtacca ggagagttta tctagatata atgagcaaga attatacatt
    11341 tccaaagcta tggtaaaagc cgaagaaaat gcacaggcca ttttgaagga gagccatcat
    11401 aagataggta tcgaaaagtt taagatgaac caggagaaag aaaaatggaa gactagggaa
    11461 aaggaaatca tgaatcaact tctagatttt caaaatgagg cctatgccct gatggaatcc
    11521 tttcaatcgg agattagcta ccttacatca aaggaattga tgtcaagtga actggaagtc
    11581 gatgatagcc ggacggatat aaacaaggat ctgttaaata ttagttagac atagcattcg
    11641 gtgaaacata gtatatagag gctagaccca tgggcagatg ccagggtcgg gcctttttta
    11701 ttggctattt gtccaaagca tatgaaatga ttcccattta ttatccttaa tccatatgtt
    11761 acgaacaata cattatttta caatgaaagg tcctcttcgc agcatatcta tgttaagata
    11821 ataatataag aggtatggct ttgccaactg gcaggtatag ggcgagacca actaatttat
    11881 ccttgatgga aattatcaaa aacataggag gaaaatatgg caaaaccaaa tattctattt
    11941 attatgtccg acgatcatgc ttctcaggcg attagtagct attcggggcg aataaaccag
    12001 accccaaata ttgacaggat cgccaatgaa gggacccggt ttgataattg tttttgtaca
    12061 aactccatat gcgcccccag tagggcagta atcttaacgg gtaaatacaa ccatttaaat
    12121 ggggtgatga cccttttcga tgactttgac ggaaggcaag atcatgtagc aaagctacta
    12181 caagataccg gctatcagac ttcgattata ggcaaatggc acttaggtca cgggggtaac
    12241 agcgatccca caggatttga ttattggtgt gtgttaccgg gccagggtga atatcacaac
    12301 cccgttttct acgatatggg cgaggagata aaggtagaag gctatgccac ggacattatt
    12361 acggatatgt ctatagattg gatggacaag cgggacaagg atcagccctt ctttttaatg
    12421 tgtcatcaca aggcacccca tcgcccctgg gagccttcgg aaaagtatat ggacctatac
    12481 gaagacatcg atataccgga acccgatacc ctgtttgatg actatgatac aagaagcgat
    12541 gctgctagcc tggccaagat gaggatcagc catcacctaa ctaagaccga tgtaaaacaa
    12601 gatccccctg agggtttaga gggcgtggaa ttgaccagat ggtactatca aagatacata
    12661 aaggactatt taaggtgcat tgcctctatc gacgatagcg tcggaaggct tttagactat
    12721 ttggacgagg aaggaatagc ggataatacc atagtgatct atacctcgga tcaaggcttt
    12781 ttcttgggag agcatggatg gtatgacaaa cgatttatgt atgaagaatc cctaaggatg
    12841 ccatttctcg tacgctaccc aaaagcaata aagcctggca ccataaacga ggatatgata
    12901 gttaataccg actttgcgcc cacattttta gactatgcag ggctagatgt accgaaggat
    12961 atgcagggct atagccttcg tcccatattt gaagggaata ccccaaacca ttggagacaa
    13021 tccatgtact ataggtattg gatggcccaa gacgatcatt gtgttgcagc acattatgga
    13081 atccgtaccc ataaatataa gcttatatgc tactatgctc ccgggtttca ttataatggg
    13141 gaatatccaa aagagcttga gggggtagaa tggccccaat gggagctgtt cgatcttgaa
    13201 agggatgggg atgaactcaa taacgtatac gatgaccctg aatattccgg tgtagtggag
    13261 gagttaaagg agaagctcta tagcctccag atggaattaa aggatgacaa ggacgccagg
    13321 tatgagaaga gggaaatata acggggcagt ggacatatac tccccacacc gttgaaactc
    13381 tgtgttgtaa ttgctgaagc agttgcttcc cacgttataa tatttactat ggggatagag
    13441 caaaacatag cagaaatagt atgaagcagg ggccgcataa gatgagcccc tgctttgttt
    13501 aggccacagg ttctatattt ctgggttcct tgcaaaatgg acaaaaagtg atacaatatt
    13561 aaacgtgcta ataggggatg cggctatatg acgagtcggc aaaatatccc ctgcttctat
    13621 aaacggcgag ttccgattag taaagcaggc ggtgggtata aatcacccgc ctcgttgaaa
    13681 cctgtgtgat gcaatcgcca atgcaattgc ttccgatgtt ttaagtcgtg gggattcaac
    13741 tatatcgcca tttggtgatg gtatatacca cctttccatt taattttaat ttttttgcta
    13801 aaacagcggt ttttgtagca gcataagata ttttcataat atgtaagcag ggcatcggag
    13861 ggaaattata ttggacaaga gggtaaatat gaaattattg acggatatat tcataacttt
    13921 ttttaagata ggatcattta cctttggcgg gggttatgcc atgatacctc taatagaaag
    13981 ggaagtgata actaacaagg gctggatcga agatgaagag gagataatcg atgtctttgc
    14041 ggtttcagaa tccatacccg gtgccatcgc cataaattct tcaacctttg tgggatataa
    14101 gatagcggga agaaagggcg ccatagctgc catgtttggg gtagtgttgc cttcatttat
    14161 tattattaca cttattgcag cctttttctc caagttccag gataacgaag ttgtaaaggc
    14221 gacttttatg ggtataaggt cggcagtagt ggcccttatc ctaatggctg ctatcaatgt
    14281 aggcaaaaaa gccataaagg acaagttctc tgccgccata tcggcgacaa ctgtaattct
    14341 ggtactggtg gcagatatac atgccatatt cgctattata ggtggtgttt tggcgggatt
    14401 aatcatatac tttacttccc ctaaaagatt ggcacatatc aaaggaaaag ggggagagga
    14461 taattgatat atttaagact cctcctaacg tttgtaaaga taggattttt tagcttcggc
    14521 gggggttatg caatgattcc aatgatagaa agggagatag aggggcatgg ctggttaaat
    14581 gctttggagt tttttgatat cgtggccata gccgagatga ccccggggcc catagcagta
    14641 aattgcgcta cctttgttgg ttataagact gcaggatttt taggaggatt tgccgccacc
    14701 cttggggtgg ccataccctc actggtcctt atactcataa tttccagata ctttttcaaa
    14761 tttcaagacc atcccctaaa cgctgccgta ttctatggga tcaggcccgt aatagccggg
    14821 ctcattgcaa cagcggcggt ttttgtgtca gagaccgcga tttttaaagg gaaactctca
    14881 ataaatctta tttcccggct ccttcgcagt ccgctggatg tcttaaattt gggaagtata
    14941 tttatatttg ttatttcatt tatcattctg aagaaaacca agttccatcc catgttggtc
    15001 atttccctgt ccggggcttt gggaatactg tttttttatg tactttaacc tgttgcgcta
    15061 gttagcatat tggtaatggc tattgaatgc tctaaacatt aataataaaa cgatgtaaag
    15121 gcgggataaa atgccaccgg taagccttct tataaaacct gcatccagca attgcaatct
    15181 tagatgcaag tattgctttt atcattccat agccgagaat agggatgtta aatcctatgg
    15241 gattatggac ttagacactt tagagaccct ggtcaaaaaa actctggagt ttgcagatgt
    15301 cacttgtacc tttgcattcc aaggtgggga gcccacttta gcggggctgg agttttttga
    15361 aaaactgata gagtttcaaa agaaatataa caaaaaggat gtccagatca ataatgctat
    15421 acagaccaac ggcatggtca tcgatgaaag atgggcaaaa tacctgcacg ataacaattt
    15481 tttagtggga ttgtctttgg atggaaccaa ggacgtacat gatatgggca gggtagaccc
    15541 cagaaatagg ggtaccttta acaatgtcat gaaaaccgct ggtctgttca acaaatacaa
    15601 tgtggaatat aatatgctat ttgtagtaag ctccggcgta gctaggcata taacta
//
LOCUS       DASRNX010000072         4763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_14406,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000072 DASRNX010000000
VERSION     DASRNX010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14406"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1426
                     /locus_tag="VFD89_02940"
     CDS             35..1426
                     /locus_tag="VFD89_02940"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HZJ57176.1"
                     /translation="MAKEVSMDKIVALCKGRGYIFPGSEIYGGLANTWDYGPLGIELK
                     NNVKSAWWKKFIQESPYNVGMDAAILMNPKTWEVSGHVAGFADPLMDCRECKARFRAD
                     NLVEDFMKEQGDDNPQTDGWDVEKLEDFVRNNSIPCPQCGKHNFTDIRYFNLMFKTFQ
                     GVTEDSQSELFLRPETAQGIFVNFRNVLRSSRKKIPFGIGQIGKSFRNEITPGNFIFR
                     TREFEQAELEFFCKPGEDMEWFRYWKDFCKDWLLKLGVPEDKLRTRDHSQEELSHYSK
                     ATTDFEYLFPFGWGELWGVANRTDFDLKQHGEHSGENFTYIDPVTNERYIPYCVEPSV
                     GVDRMVLTFLCAAYCEEELEGGDTRVVLRLHPYLAPYKAAVLPLSKKLKDQALEVYSS
                     LSKKFSVDYDESSSIGRRYRRQDEIGTPYCITIDFDTLEDNCVTIRDRDTMDQIRLPI
                     DEVEKYLEEKVSF"
     gene            complement(1454..2005)
                     /gene="loaP"
                     /locus_tag="VFD89_02945"
     CDS             complement(1454..2005)
                     /gene="loaP"
                     /locus_tag="VFD89_02945"
                     /inference="COORDINATES: protein motif:HMM:NF033641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator LoaP"
                     /protein_id="HZJ57177.1"
                     /translation="MNWYAIFVETGREAEVQKFIELLFPDEEIRTLVPKRKLIERRQG
                     KTYESIKNLIPGYVLTHTKMSAELYYGLRALPSVYRILKDDCEPIPIRKDEVDMILGL
                     TQGGEVIELSDIYKEGNHIKVLSGPLMGMEGIIEKFDHRKKRVKVCLEFLGEYKRVDL
                     GANMIRPQEFGKTNKNVKGRKPQ"
     gene            2414..3955
                     /gene="spoVB"
                     /locus_tag="VFD89_02950"
     CDS             2414..3955
                     /gene="spoVB"
                     /locus_tag="VFD89_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein B"
                     /protein_id="HZJ57178.1"
                     /translation="MDKNTIIKGTFILTLSNILIRLLGFVYRVLLSRIIGPEGMGVLQ
                     LVMPILYTSLALISAGIPIAVSRLVAQKRAVGDYHGERNILFTSLGLVISLSLFVCMF
                     LIPNVNYIAKDLLMEPRAYGPLLALYPAIIMMSISSVFKGYFYGVKNFYPPALSELVE
                     EIITMVLVLYLIEKVKHLEVSLQVTAVAIAIVLGELSSLLFLSHSCHRYRNTIPLSRN
                     RIRSPSPVGDILKISGPVTLIRLVSSLSTSVGSILIPQRLVKSGLAHSQAMSLLGILN
                     GMVVPLIFIPFTFVSPLAVVIIPNLSEDLARKNWERIRSKISKAIFITIVAVLPFGAI
                     MAALAHPIGILLYNQKGVGSILELMAYFSGINALSHTLSGILNGLGKQNKSAMFSIIG
                     ETIEVMSIYFLVALPDLRIYGYIIGFLFSGLVVLLMQFVTVYKTAKLSIDWSRWFLKP
                     VLASLLTISLSRLAYTWLYSGTRNLAPSFFLSLLVGILTYMLSLYITGIFSAIKSLLL
                     PARRA"
     gene            complement(3964..4533)
                     /locus_tag="VFD89_02955"
     CDS             complement(3964..4533)
                     /locus_tag="VFD89_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57179.1"
                     /translation="MDRNYTIYILLTYSGTLPSKFIRMYTREPYSHVSIALDSDLREL
                     YSFGRLKPSNPIIGGFVRENILYGTFGRFPETQCALYSLTINSYQYYRLKAELNKFLM
                     SGKRYGYNFLGLLGVMVGVPIPRTYKYFCSQFVATLLTNSGIDFFQKHPALVSPMDFR
                     MSSKLTLIYEGKLRAYGSLTPYGDVLSGV"
BASE COUNT         1277 a         1100 c         1012 g         1374 t
ORIGIN      
        1 cttttttatt ttaaattttt aggaggaatg agaattggca aaggaagtca gcatggacaa
       61 aatcgttgcc ctgtgcaagg gccggggtta tatcttcccc ggatcggaga tttacggcgg
      121 gctagccaac acctgggatt atgggcccct gggtatagaa ttgaagaata atgtgaaaag
      181 cgcatggtgg aaaaagttta tccaggagag cccctataac gtgggcatgg atgcagctat
      241 ccttatgaac cctaagacct gggaagtgtc tggccatgtc gccggttttg ccgatccgtt
      301 aatggattgc agggaatgta aagccaggtt ccgagcagat aatctggtgg aagattttat
      361 gaaggagcag ggcgatgata atccccaaac ggacggatgg gatgttgaaa aattggaaga
      421 tttcgttcga aataacagca taccatgccc ccaatgcggc aagcataact ttaccgatat
      481 aagatatttt aatcttatgt tcaagacctt tcaaggggtt accgaagatt cccaatccga
      541 gctgttttta aggcctgaga cggcccaggg tatcttcgtc aactttagga acgtgcttcg
      601 ttcatcccgc aaaaaaatac ctttcggtat aggacaaatt ggaaaatcct ttagaaatga
      661 aatcaccccg ggaaacttca tcttcaggac cagggaattt gaacaagcag agctggaatt
      721 cttttgtaaa ccgggagagg atatggagtg gttccgttac tggaaggatt tttgtaagga
      781 ctggctcctt aagctgggcg ttccggaaga caagctccga actagggatc atagccagga
      841 ggaattatcc cattatagca aggcgaccac ggattttgaa tatctattcc cctttgggtg
      901 gggcgagctt tggggtgtag ccaatagaac ggattttgat ttaaagcagc acggtgaaca
      961 ctccggagaa aacttcacat atatagaccc tgttaccaac gagcgctata ttccctattg
     1021 tgtagagcct tcggtgggcg tagacaggat ggtcctcacc ttcctatgcg ccgcgtactg
     1081 cgaggaggaa ttggaaggcg gagatactag ggtagtattg agattacacc catatctcgc
     1141 cccttataag gccgctgtgt tgccgttgtc aaagaagctc aaggatcagg ccctggaagt
     1201 atacagcagc ctaagcaaga aattctcggt tgactatgat gaatccagca gcataggcag
     1261 gagatatcgt cgccaggacg agattgggac cccttattgt ataactatcg actttgatac
     1321 cctagaggac aattgtgtta ctattcgtga tcgggatact atggatcaga tcaggttacc
     1381 catagatgag gtagaaaaat acctggaaga aaaggtttca ttttaatgtg aagatgcaaa
     1441 ggccggcaag ccattattga ggcttccggc cttttacatt cttattcgtt tttccaaatt
     1501 cctgcggtct gatcatattt gcgcctaagt ccaccctttt atactcaccc aaaaactcca
     1561 gacatacctt gacccttttt ttcctatggt caaatttttc gattatccct tccatgccca
     1621 tgagcggacc gcttaggacc ttgatatggt tcccttcctt gtagatatcc gataattcaa
     1681 tgacttctcc gccttgggtc agaccgagta tcatatccac ttcatccttc cttatgggaa
     1741 taggttcgca atcgtccttt aaaatcctgt acactgaggg aagagctctc agtccataat
     1801 atagctcggc gctcatcttt gtgtgagtca aaacataccc gggtatcaag tttttgatgc
     1861 tctcataggt ctttccctgt ctgcgctcta tgagcttcct tttgggaacc agggtacgaa
     1921 tctcttcgtc cggaaacagg agctctatga acttttgcac ctctgcctcg cgtccggtct
     1981 caacaaatat tgcataccaa ttcatgtatg acctccaaat atatgtttta tgatccatct
     2041 tatatcttgt tttagttatc gcaaaatgtc cccatgcatt tacatttgct gccgatgttg
     2101 taacgagtcg gcatgatgtc tcccgcttct ataagcggcg agttcccatt agtaaaacaa
     2161 ggcggtgggt acaaatcacc cgccccaatg cgatggatta gcgtgtagta caaataatct
     2221 ggtgccatta tctaccgccc ctatgattag aagctgtaaa tcccattgta atacactata
     2281 tgtccaaaga caaactaata atggtcatgt ccagctgggc tccgcccccc gttaccggct
     2341 ccccggctta tctatggact tctattttcc catatccatg aatactatat tcacatacag
     2401 aaggggtggt tttgtggata aaaatactat tataaagggt accttcatcc tcaccctgtc
     2461 aaatatatta atacggctgc tgggatttgt gtatcgcgta ttgctgagca gaataatagg
     2521 gcccgagggt atgggcgtat tacagctggt aatgcctatc ctatatacct ccttggcgtt
     2581 gatatcggcg ggcattccca tagctgtatc ccggctggta gcccaaaaaa gggccgtagg
     2641 ggattatcat ggcgagcgca atattctctt caccagcctt ggattggtaa tatccctatc
     2701 cttatttgtc tgtatgtttt taattcccaa tgttaattac attgcaaagg atcttttaat
     2761 ggaaccgcgg gcatatggac cgctacttgc cctatatccc gcaataataa tgatgtccat
     2821 cagctccgtc ttcaagggat acttctatgg agtcaaaaac ttttatcccc ccgcattgtc
     2881 ggaattagtg gaggagataa tcaccatggt gttggtgtta tatctcattg aaaaggtcaa
     2941 acatttagag gtttccctcc aggtaactgc cgtagccata gctatcgttt taggggaatt
     3001 gtcatccctg ctgtttttaa gccatagctg ccatagatat cgcaatacca taccgctatc
     3061 aagaaatagg attaggtccc cttcccccgt gggggatata ctaaaaatca gcggtcccgt
     3121 aaccttaatt cgcttggttt cttccctgag cacatcggtg ggctccattt taattcccca
     3181 aaggttggtt aaatccggtc tcgcccacag ccaggccatg agccttttgg gcattttaaa
     3241 tggaatggtg gtccccctca tatttattcc ctttacattt gttagccccc tggcagttgt
     3301 cattatccca aatctatctg aggatttggc acgtaagaac tgggagagga tacgctctaa
     3361 aatatctaaa gccatattca taaccatcgt cgctgttttg cccttcggtg ccatcatggc
     3421 ggcccttgcc caccccatag gcattttact atataaccaa aaaggggtcg gaagcatact
     3481 ggaactcatg gcatatttct ccggaatcaa tgcgctgagc cataccttaa gcggtatctt
     3541 aaatgggctg ggaaaacaaa ataaatccgc catgttctca attataggag agaccataga
     3601 ggtaatgagc atttatttcc tggtagccct ccctgatttg cgaatatacg gatatattat
     3661 cgggtttcta ttctccggcc ttgtagtcct attgatgcaa tttgttaccg tatataaaac
     3721 tgccaagctg tcaattgact ggtcgagatg gtttttaaag cccgtgcttg catcacttct
     3781 taccatctct ttatctaggc ttgcctatac atggctctac agcggcacaa gaaaccttgc
     3841 cccgtccttc ttcctatccc tacttgtggg tatccttacc tatatgctaa gcctttacat
     3901 aaccggcatt ttttcagcta taaagagcct gcttttgccc gcacgacgtg cgtaacaccc
     3961 gccctagacc cccgatagga catccccata gggtgttagg gatccatacg ctctcagctt
     4021 tccctcatat atcaatgtta acttgctgct catcctaaaa tccattggag agactagggc
     4081 gggatgcttt tgaaaaaagt ctattccgct atttgtcaaa agggtggcaa caaactggga
     4141 acaaaaatat ttataagtcc ttgggatggg tacccccacc attacgccta gcaggccgag
     4201 aaaattatac ccgtatcttt tcccgctcat caaaaattta tttagctcag ccttcaacct
     4261 gtagtattga tagctgttta tagtcaatga atacagggca cactgggtct ccggaaatct
     4321 cccgaaggtc ccatacaaga tgttttccct gacgaatcct ccaataatgg ggtttgacgg
     4381 tttcaacctc ccaaagctat acaactccct tagatccgaa tccaatgcaa tagatacatg
     4441 ggaatagggt tccctagtat acatccttat aaacttagag ggtaacgtac cgctatatgt
     4501 cagcaatata tatattgtat aatttctatc catgtcgatc ccctttaaat aatttaacac
     4561 gtcattccag ttaagcatgt cgataatggc tattgaacta gcacaacgcg ttaactgata
     4621 taccccaatc aataaagttg ataaggtgcc ctaatatatg aattttatat actaggccac
     4681 cgaaattata tagataggca accccaacta caaaatttat tacctatgga taaattttgt
     4741 tttcgtcttg tctatctata acc
//
LOCUS       DASRNX010000073         5229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_228665,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000073 DASRNX010000000
VERSION     DASRNX010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228665"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VFD89_02960"
     CDS             complement(<1..636)
                     /locus_tag="VFD89_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ57180.1"
                     /translation="MEWRPKQKPKEVRIAFIGTGIQQRSHMRSYENIPGVKYVAACDI
                     NEDKLNEFCDDYNIEHRYLDYREMLKRDDIDAVDVTVHNNLHLPLVVEVLRSGKHCYC
                     EKPMAGSFADAVTMYRASEDYGKMLHIQLGRIYDTATVAAKEYVDAGKLGKIYHARSY
                     GYRRRGRPYVDGYAEKEFDQAWIAQHGALFDMGVYHISQLLYLLGLPKLERV"
     gene            complement(815..2071)
                     /locus_tag="VFD89_02965"
     CDS             complement(815..2071)
                     /locus_tag="VFD89_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZJ57181.1"
                     /translation="MIQNNFNDVPAEEKTSEKRSEFQFYKYAALNFVFYITISLGGYV
                     TIFLQSIGFDAQQVGIITALNSGVGAFSSPIWGMLGDKIRSVRKVINIILVISTVLFA
                     FIPMSKGINIGGISFLFILIPITMFFKMPIMSLVDNWVLENSVHERLNYGALRAFGAL
                     SFAIASLALGYILPITGVEFTFYASAVVTIPALLMIIYFKTARDDVASKGHLTLKEMQ
                     ISKLFKNYYLIAYIIFTAFQRISFHCSMIFLPFLVTAVDGNIAQMGIVMAIRAFMEIP
                     MMLLLKPLRRRLPLYHIIFISSGFFIIECIMYSFANSFAMVAAISVLHGIGNGLMLPT
                     SISYVFSLAPDNLKATSQTLLGSTNSIAGVLGGILGGVLITIFGVKQFYLVIGLMMLV
                     TLALYLLSFVFGEKVLGKSRPGLSID"
     gene            complement(2382..3227)
                     /locus_tag="VFD89_02970"
     CDS             complement(2382..3227)
                     /locus_tag="VFD89_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZJ57182.1"
                     /translation="MSNSAKGINSLPPGWGENQQAVTETKLPLATQLFPVADMLSKDL
                     RGTLKLVKEAGYDGVEFAGGLKYAAQDVRYALDETGLQIVGWHTPWDYLSPDNIYSTI
                     TYNKVLGNQFIIVPWMPDETLLTRESCLKFAAELTWVSDVLGMYGMVTGYHNHATEFR
                     PTDDTDELPWDIIAQNTPSNVIMQNDIGNGMGGGGDMIDMLRKYPGRGVTIHMKPYSS
                     EGRDTFFDDPNCTIDWDEYFEIARKEAGVRWYIVEYVNNVRFPNDPIAGLAASAKWFR
                     GRNGR"
     gene            complement(3484..4761)
                     /locus_tag="VFD89_02975"
     CDS             complement(3484..4761)
                     /locus_tag="VFD89_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HZJ57183.1"
                     /translation="MNRYMCILLCAVVVMGVLIGCSSNPLANPSRVGGIKDSGEPDIQ
                     DTNLVYDSEKISMDMIEGTTEFAFDIFRQLNREDMDGNIFISPLSISTVLSMTYQGAG
                     GETQESMAGVLGYSDIDINILNESYKNLLRLLMDQEKGVRLNIGNSIWIREGKPIEGD
                     FLAVNKDIFKARIETLDFSNDRSAEKINKWVSHATDGKIEKIIEPPIAPETIMYLINA
                     VYFKGDWTDKFDKNKTFESKFYAGDGSPRDTMMMTREGLIQYGQGEDYTAVRLPYGNE
                     TMAMYCVLPKGESPINDFVEGLNSEKWRAIRESTAEMDGVMLNLPRFKMEYGIKSLKE
                     SLTALGMGNAFTYDANLSGIGQELFISDVLHRAVVEVNEEGSEAAAVTAVDLKEEAAA
                     QPHEFIADRPFFFAIIDDKTGLILFMGKVYDLS"
BASE COUNT         1472 a         1191 c         1095 g         1471 t
ORIGIN      
        1 taccctttca agcttcggca ggcccaagag gtagagaagc tgtgagatat ggtaaacgcc
       61 catatcaaac aatgcaccgt gctgggctat ccatgcctga tcaaattctt tttccgcata
      121 tccgtcgaca tatggacggc cacggcgacg gtagccatag gagcgggcgt gatagatttt
      181 gcccagctta ccggcgtcta cgtattcctt tgcggcaacg gttgcagtat cgtagatgcg
      241 cccaagctgg atgtgtagca tcttgccgta atcttcgctg gccctataca tagtgacggc
      301 atcggcaaag gatcctgcca tgggtttttc gcagtagcag tgtttcccgg agcgcagtac
      361 ttcaacgacc aagggaaggt gtaaattgtt atgaaccgta acgtccacgg catcgatgtc
      421 atccctcttt agcatctcac ggtaatctaa atagcggtgc tcgatattat agtcgtcgca
      481 gaattcattg agcttatcct cattgatatc gcatgcggcg acatacttta caccgggaat
      541 attctcgtag ctcctcatat gggaacgctg ctgaatacca gtacctataa aggcgattcg
      601 aacttctttt ggtttctgtt taggtctcca ttccattttc tttaccccct aagatatttt
      661 ttatttaaaa ccggcatggg ccggattttc gcaagtatcc aaatctatag gacactgaat
      721 aaaacatcga tagccttggg atacacgctc cagcgggtag taaccagccg gtattgttct
      781 ctgccatacg tccattgatc gaatagacgt ttttttaatc aatactcaaa ccgggccgac
      841 tttttccaag gaccttctcc ccaaaaacga agctaagcag atataatgca agggtgacta
      901 acatcataag accgattacc aaataaaact gctttacacc gaatatagtt atgagaaccc
      961 caccaagaat tccccccaat accccggcaa tagagttcgt agatccaaga agtgtttggg
     1021 atgtagcctt caggttatca ggggcaaggg aaaagacata gctgatactg gtaggcagca
     1081 taagcccatt gccgattccg tgaagcactg atatcgcggc gaccatggca aagctgttgg
     1141 cgaagctgta cataatgcac tcgattataa aaaaaccgga tgatataaaa ataatatgat
     1201 acagcggaag tcttcgcctg aggggcttta acaataacat catgggtatt tccataaagg
     1261 cccttatagc catgacaatg cccatctggg cgatattccc atctacggcg gtcacaagaa
     1321 agggaagaaa tatcattgaa cagtggaaag aaatcctctg aaacgcagta aaaataatgt
     1381 aagcgatcag ataatagttt ttgaacagct tgcttatctg catttcttta agggtaagat
     1441 gtcccttact cgcgacatca tccctggcgg ttttaaagta gatgatcatt aaaagtgccg
     1501 ggatggtaac gaccgcactt gcataaaagg tgaattctac cccggttatg ggcaggatgt
     1561 atccgagggc caggctggca atcgcaaaac tgagggcgcc aaaagcgcgt agggcaccat
     1621 agttgagcct ttcatgaacg gaattttcaa gtacccaatt atctaccagg gacattatgg
     1681 gcatcttaaa aaacattgtt atgggtatga gaatgaataa aaaggatatt cccccaatat
     1741 ttatcccttt tgacatgggt ataaaagcga acaaaacagt gctaatgacg aggataatgt
     1801 tgattacctt tcttacagaa cgtatcttat cacccaacat accccaaatg ggagaggaaa
     1861 aagccccgac tccggaattt aacgctgtaa taattcccac ctgctgcgca tcaaatccaa
     1921 tggattgaag aaatatcgta acgtagccgc cgagtgaaat tgtgatatag aagacaaaat
     1981 tcaatgcggc atatttatag aactggaatt ccgatctctt ttcacttgtt ttctcttctg
     2041 caggcacatc gttaaaatta ttttggatca cctatttagc tccttatcct taatatattg
     2101 atggcaatag gaattgcttt gaatatcatc aaataaataa ccgatcaatt ggagtctcaa
     2161 agaggctgga ctatcctttg aagcttcaaa ggggctgaat tgccctttga ggtttcagag
     2221 tggctgaatt atccttgctg tgttaccgag aataggggta tgggatttgg ctgggagtat
     2281 actatggtat atctccaacc aaatcctaca ttaaactgca tgcgtccctg tattaaattg
     2341 taagtacctc tatactgaat tgtaagtgtg ctttttaaat actatcttcc gttacgaccc
     2401 ctaaaccatt ttgccgatgc agcaaggccg gcgatgggat cattggggaa ccggacgtta
     2461 ttgacgtatt ctacgatgta ccagcggacg cctgcttcct tgcgggcgat ctcaaaatat
     2521 tcgtcccaat cgatggtgca gttgggatca tcaaagaagg tgtcccggcc ttcggatgaa
     2581 tagggtttca tatgtatggt taccccacgg cccggatatt ttctcagcat gtcgatcata
     2641 tccccgccgc cacccattcc atttccgata tcattttgca tgatgacgtt ggatggggtg
     2701 ttttgggcga tgatgtccca gggcagctcg tcggtatcgt ctgtaggtct aaactccgtg
     2761 gcatgattat gataaccagt gaccatccca tacatgccca gcacatcgga aacccaggtc
     2821 aattccgcag cgaattttag gcagctctcc ctggttaaaa gggtctcgtc cggcatccaa
     2881 ggaacgataa taaattgatt tcccaatacc ttgttatagg tgatggtgga atagatattg
     2941 tccggggaca gataatccca cggcgtatgc caacccacga tctgcaatcc ggtctcgtca
     3001 agggcgtagc gaacatcctg ggcggcgtac tttagcccgc cggcaaactc cactccgtca
     3061 tacccggctt ctttaaccag ctttagggta ccccgaagat cctttgaaag catatccgcc
     3121 acgggaaaaa gctgcgtggc aaggggcaat tttgtttccg ttaccgcctg ttgattttct
     3181 ccccatcccg ggggcaaact gttaatacct ttggctgagt tgctcatatg ctcatcctcc
     3241 tatatgattt ttagaacaag aacacaaggg gacggtcctt ctgtgttctt gtgtcgtccc
     3301 ctgtgtttac catgtatacg atagcttccg ttgccttaat tatacacaac atgtcctgaa
     3361 taaacaatgt gttgagcaaa caaaaatata cgcttttctt tcatttacac cggtgtgatc
     3421 tatagcgctt catcgatgtc ggtctgtcag gctatggcaa ataatcgact ctatagttat
     3481 ggattaactc aaatcatata ctttccccat aaacaagata agtccggttt tatcatcaat
     3541 gatagcaaag aagaatggcc ggtccgcaat gaattcgtgt ggctgagcag cagcttcctc
     3601 ttttaaatct accgccgtga cggctgcagc ctcgctgcct tcttcgttga cctcgactac
     3661 ggctctgtga aggacatcgc tgataaaaag ctcctgacct atacctgaga ggttggcatc
     3721 gtaggtaaag gcgttgccca tgcccagggc agtgaggctt tccttcaggc ttttaattcc
     3781 gtattccatt ttaaagcggg gcagattaag cataactccg tccatttcgg ctgtactttc
     3841 ccttattgcc ctccactttt cgctgttcaa accttctaca aaatcgttga tgggggattc
     3901 accctttggc agcacacaat acattgccat agtttcgttt ccgtagggca atctcacggc
     3961 tgtataatct tcgccttgtc catattggat cagcccctcc cgggtcatca tcatagtatc
     4021 tcggggacta ccatcacctg catagaattt ggattcaaag gttttatttt tatcgaattt
     4081 gtcggtccag tcccctttga agtaaacggc attaataagg tacataatag tctccggtgc
     4141 aatgggcggt tcaataatct tttctatttt accgtctgtg gcatgggaaa cccatttatt
     4201 gattttttct gcagaccgat cattagaaaa atccaacgtt tctatccggg ctttgaaaat
     4261 atccttgttg acagcaagaa aatccccttc aatgggtttt ccttccctga tccaaataga
     4321 atttcctatg tttaacctta ctcccttctc ctgatccata aggagacgca ataggttctt
     4381 ataactttca tttaggatat ttatatctat atcggagtac ccaaggactc cggccatgga
     4441 ctcttgtgtt tctccgccgg ccccctgata ggtcatggaa agcactgtgg agatactcaa
     4501 aggggatata aatatattcc catccatatc ttctcggttc agttgtctga agatatcgaa
     4561 agcaaattcc gtggttccct cgatcatatc catactgatc ttttctgaat catataccaa
     4621 attagtatct tggatgtcgg gttcgccact gtctttgatg ccgcccaccc gggaagggtt
     4681 ggcgagggga ttggacgagc agccgatcaa tacacccata accactaccg cacagagcag
     4741 aatacacata tacctattca taaatatacc tcctaaaaca ttcttatgat tatcaccctc
     4801 aaagtaagtc ctgtttgaat cagtcatttg ttctaatagc cggggcagaa ctaaaatttt
     4861 taaggcaata atccttccta ttaatacaag acgtatatat tagggatttg ttccaggaag
     4921 attggctgat atacccagat ccggaaaagc ggaaatatag ataaacagcc ccggctataa
     4981 aaccttaaat aaaggtctaa aatatttcaa aataagcatt gacaaatatt taacaagggt
     5041 ttataataac attaccaaga cggattaagt agtttggcaa ataaataaga atatagtggg
     5101 aatatgggtt aatagattaa aataatttta aacagctata tataaccgac aaagactgag
     5161 accagagata tttttttaat aaacatagtt aaataattaa caatactgca acaaaccaca
     5221 tactattac
//
LOCUS       DASRNX010000074        27168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_544813,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000074 DASRNX010000000
VERSION     DASRNX010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 27168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27168
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544813"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(779..2437)
                     /gene="ilvD"
                     /locus_tag="VFD89_02980"
     CDS             complement(779..2437)
                     /gene="ilvD"
                     /locus_tag="VFD89_02980"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZJ57184.1"
                     /translation="MISDRVKKGYEKAPHRSLFKAMGYTNEELALPLIGVVNSQNEVV
                     PGHIHLDTIADAVKNGIRMAGGTPIEFPSIGVCDGIAMNHVGMNYSLASRELIADSIE
                     AMTMAHGFDGLVLIPNCDKIVPAMLMAAARLNIPCIVISGGPMLAGKKRGKSLDLNSV
                     FEGVGAVSAGVMTEEELYEIEEDACRSCGSCAGMFTANSMNCLTEALGMGLPGNGTVP
                     AVYAQRIRLAKRAGMKILELVEKNICPRDIMTESAFQNALTVDMALGCSTNSVLHLTA
                     IAHEAGVKMNLDIINEVSMRTPNLCKLSPAGEHRIEDLYAAGGVQAVIKELSKEDLMD
                     LDLMTVTGHKMGANIEDAQVLDYDVIRPIEDPHSKTGGIAVLRGNLAPDGAVVKRSAV
                     APEMMVHEGPARVFDSEQDAFAAILGRKIQKGDVIVIRYEGPKGGPGMKEMLSPTSAI
                     AGMGLDKDVALLTDGRFSGATRGASIGHISPEAMEGGPIAAIHEGDIISINIPGGLLE
                     VRLSAEDLKSRLEQWNAPEPKIKNGYLARYARMVSSADKGAILT"
     gene            complement(2592..3476)
                     /locus_tag="VFD89_02985"
     CDS             complement(2592..3476)
                     /locus_tag="VFD89_02985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZJ57185.1"
                     /translation="MNTVRYRVGSRRRYRINGKRFYPLLALFIILVILVILNRVQEQG
                     FYTKYRIAEFDSEHLGVYFDGGDAQGVPWYYLLAIDKAEEIPKEEVSPGRSEQIALHL
                     RGIASPNELSKKLKDYNNDGKFIGRVKNEIKCLEYIIGVYDDKVFPISGHDYHYEDGY
                     GDGRSYGGERRHEGIDLMCDEGTPLLAVCDGVIEKKGWLELGGWRIGIRGNDGVYYYY
                     AHLSRYEDKLEKGHRVNKGQIIGYAGDTGYGKEGTTGQFAPHLHFGMYERDGWTGSGE
                     KAVNPYPFLRAWERSGEK"
     gene            complement(3640..4704)
                     /gene="leuB"
                     /locus_tag="VFD89_02990"
     CDS             complement(3640..4704)
                     /gene="leuB"
                     /locus_tag="VFD89_02990"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZJ57186.1"
                     /translation="MRIALIPGDGIGPEIIAQGLKALDAVSRKYDVEFTFVETLMGGA
                     AIDKTGVPLPEETVKVCRDSDAVLLGAVGGEKWDSLPGHLRPEAGLLGIRKALGVFAN
                     LRPAILFPQLKEASSLKAEVLGDGLDIMVVRELTGGLYFGERGRAGQGDSAKAWDTME
                     YSVSEIERIARVAFELAERRNKKLMSVDKANVLESSRLWREVVGAIASDYPEIELSHM
                     YVDNAAMQLIRNPKQFDVIVTENMFGDILSDEASMLTGSLGMLPSASLGDKNPGIYEP
                     IHGSAPDIAGRDKANPIATIMSVAMMLRYSGDVEEGARDIERAVSSVLDMGYRTGDIM
                     QPGMKEIGTDLMGDLIAREI"
     gene            complement(4707..5192)
                     /gene="leuD"
                     /locus_tag="VFD89_02995"
     CDS             complement(4707..5192)
                     /gene="leuD"
                     /locus_tag="VFD89_02995"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZJ57187.1"
                     /translation="MKGTVIKYGDNVDTDVIIPARYLNISDMKELAKHCMEDLDKDFV
                     GKVKPGDIMVAGKNFGSGSSREHAPGVIKASGISCVIAETFARIFFRNAINIGLPIME
                     SPEAAKDIEDGDQVSIDVYSGVITNITKNRTYQGTPFPGFMQDIMRAGGLINYVKEKG
                     E"
     gene            complement(5189..6457)
                     /gene="leuC"
                     /locus_tag="VFD89_03000"
     CDS             complement(5189..6457)
                     /gene="leuC"
                     /locus_tag="VFD89_03000"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZJ57188.1"
                     /translation="MGMTMTQKILAAHAGLDRVEAGQLIMANLDLVLGNDITSPVAIK
                     EFNKIGVGEVFDKSKVAIVPDHFTPNKDIKSAEQAKFIREFAGDKEIENYFEIGEMGI
                     EHALIPEKGLVVGGDVVIGADSHTCTYGALGAFSTGVGSTDMAAGMADGRAWFKVPEA
                     IKIVLTGSLPKWVSGKDVILHIIGMIGVDGALYESMEYTGPGVASLSMDDRFTMANMA
                     IEAGAKNGIFEIDDKTIAYMKEHSAKEYTVYKADEDAEYTRVIEVDLSALRPTVAFPH
                     LPDNTRTIDEAGDVPIHQVVIGSCTNGRIGDLRVAAKILEGRRVKKGVRTIIFPATQK
                     IYLQAMQEGLLEIFVEAGAAVSTPTCGPCLGGHMGVLAAGERAVATTNRNFVGRMGHP
                     DSEVYLASPAIAAASAITGKISSPEEVVES"
     gene            complement(6502..8109)
                     /gene="cimA"
                     /locus_tag="VFD89_03005"
     CDS             complement(6502..8109)
                     /gene="cimA"
                     /locus_tag="VFD89_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HZJ57189.1"
                     /translation="MNKEKVFIYDTTLRDGAQAEGISFTVNDKLRIARRLDSLGVDYI
                     EAGNPGSNPKDLEFFQRMKKRPLKHARLAAFGSTRRPGIKAQDDANVKALLTADTPAV
                     AIFGKSWDFHVTEIIRTSLKENLNMIYDTVSFLKSHGKEVIFDAEHFYDGYKANSEYA
                     MGALEAAAKAGADWLVLCDTNGGCFPLEITEITSEVLKKFTIPIGIHCHNDGGMAEAN
                     SIMAVEQGVVQVQGTFNGYGERCGNANLSVIIPNLQLKRDKTCIKDEGLKELSSACRY
                     ISELANLPYNERQPYVGNSAFAHKGGMHIDGVMKNQHSFEHIAPELVGNERRILMSEV
                     SGRSTILKRIQDVAPWVNKDSEETILVMDRLKQLEHDGYQFEGAESSFELVVKRALGK
                     AKTFFEVKDFRVLCEEHWQNDYSASAIVKVKVDGSEEIIAAEGDGPVNALDKALRKAL
                     EIFYPQLKRMRLTDYKVRVLDTTKATAAKVRVHIESTDGRKVWGTVGLSTNIIEASWD
                     ALVDSIEYFLYLNGNAEGEDIGNGYGQ"
     gene            complement(8128..9648)
                     /locus_tag="VFD89_03010"
     CDS             complement(8128..9648)
                     /locus_tag="VFD89_03010"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZJ57190.1"
                     /translation="MTKRVYIFDTTLRDGEQSPGISLNVHEKVEIAAQLERLGVDAIE
                     AGFPVASKGDFEAVKAIAQSIRKPIIVGLARAVKGDIERAWEALKYAARPRIHTFIAT
                     SDIHMEYKLKMKPEEVLKQVKDAVTFAKSLCEDVEFSAEDGSRSRPEFLYRVIETAIG
                     AGATTINIPDTVGYGTPQEFGELIASIMENVKGIEDVVLSVHCHNDLGMGVSNSLAAI
                     ENGATQVECTINGLGERAGNAALEEIVMALKTRKGFYGCETGIETEELYRTSRLVSHL
                     TGVQIQPNKAIIGANAFAHESGIHQHGMLSNRETYEIMTPESVGFRQSEMVLGKHSGR
                     HAFEEKLGDMGYTHLDTEKINEAFSKFKDLADKKKIVTDRDIEALLSEKVIQVPAVYE
                     LEYFHISSGNSTISTSTVRLKVDDGLIQEAACGDGPIDATFRAIDRAAGIEVNLKDYY
                     LRAVTSGKDALGEVTVKVSRNGDIFVGRGISTDVIEASAKGYVNAINKMLYETEME"
     gene            complement(9668..10663)
                     /gene="ilvC"
                     /locus_tag="VFD89_03015"
     CDS             complement(9668..10663)
                     /gene="ilvC"
                     /locus_tag="VFD89_03015"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZJ57191.1"
                     /translation="MAIMYYDQDANMELLAGKRVAVIGYGSQGHAHALNLKESGVDVR
                     VGLYKGSRSWEEAEKAGLRVLEAGEAAGEADYIMILTPDETQAGIYKESIEPNLKEGD
                     GLIFAHGFNIHFGQIIPPDNVDVFMVAPKGPGHTVRSQYQEGKGVPCLVAVHQDYSGR
                     AKDFALAYAKGIGGTRAGVLETTFKEETETDLFGEQAVLCGGISELIKAGFETLVEAG
                     YQPESAYFECLHEMKLIVDLMNQGGLSFMRYSISNTAEYGDYSVGRRIITEDTREEMK
                     NVLGEIQDGTFAKNWILENQANQVSLKATRKKEQDHIIERVGKELRKMMPWIEEK"
     gene            complement(10644..11180)
                     /gene="ilvN"
                     /locus_tag="VFD89_03020"
     CDS             complement(10644..11180)
                     /gene="ilvN"
                     /locus_tag="VFD89_03020"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZJ57192.1"
                     /translation="MMKHILGVLVENHPGVLSKVSGLFSRRGFNIYSLAVGMTEDATV
                     SRITIVVEGDSYIVEQVTKQLNKLIDVIKVSDIGNDSVTRELALIKVKANPKTRHEIV
                     EIINIFRAQVVDITKNHLTVEITGDSEKITALEDMLKDYGIEEMVRTGAIAINRGKRM
                     LKYGDKNKGGHENGNNVL"
     gene            complement(11149..12816)
                     /gene="ilvB"
                     /locus_tag="VFD89_03025"
     CDS             complement(11149..12816)
                     /gene="ilvB"
                     /locus_tag="VFD89_03025"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZJ57193.1"
                     /translation="MQMTGAEAVVRALELEGVNIIFGYPGGAVTHIYDAFSRSKIHHV
                     LVRNEQAAAHGASGYARATGKVGVCISTSGPGATNMITGIATAYMDSIPIVAITGQVP
                     LSMVGRDVFQEADITGATEPFTKYSYLVKNPEDLPRIFKEAFYIASTGRPGPVLIDIP
                     KDIAMAPLDFEYPDEVNLRGYKPTYRGHPGQVKRMVKAIEESRRPLICVGGGAVSSDA
                     TAEIMELAERIKAPVASTLMGIGAFPGDHRLSLGFLGQHGIYAANRAVEEADLLILLG
                     ARMGDRATGKTQEFAQRAKVIHVDIDPAEIGKNIGVNIPIVGDIKTTLGELLKKDIDR
                     HDWGWLSTVRAWKQKHEGVGDCLKGLNPMEVIKKLSGMVPENTVLVTDVGQHQIWAGR
                     YYKTRKPRGFLTSGGLGTMGYGLPAALGAKLGRRDSEVVLIIGDGGLQMNLAELATLV
                     QEGAGVKIMILNNGYLGMVREIQEEIHEARYYQTRMEGNPDFIKLAQAYGIKGFRVES
                     QERIEWGIRKLLNWDGPIIGEFVIDPTANVIPILGGKDDEAHIRGIG"
     misc_binding    complement(12871..13119)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(13392..13589)
                     /locus_tag="VFD89_03030"
     CDS             complement(13392..13589)
                     /locus_tag="VFD89_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HZJ57194.1"
                     /translation="MTRQFYVGDIIETKKKHPCGNSQWEVTRVGADMKMKCLRCGRIV
                     MLDRESFEKRVKRVIEQKGQE"
     gene            complement(13618..14463)
                     /locus_tag="VFD89_03035"
     CDS             complement(13618..14463)
                     /locus_tag="VFD89_03035"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZJ57195.1"
                     /translation="MLDDIIAWLNQWTVDHYLAIGYRILRVILIWAAAVFFIRLGRFL
                     INGFFESRAKGRFHVNKKKNDTLRALVQSILRYVIYFIGFLMIMDEIGIETTSIIATA
                     GIGGLAIGFGAQSLVRDVITGFFIIFEDQYSVGDFIKIEDITGTVEEIGLRITKIRGF
                     KGDINIIPNGQIFMVTNYSRGNSAVIVDVTIAYEADIDRAIAVIDKVIEKYAAANTDI
                     VEIPRAVGIVEMDSIGLTLRVVGRTLPMKHWGVERDLRKAIKVALEENKIEIPYPRLV
                     VINRD"
     gene            complement(14530..15255)
                     /locus_tag="VFD89_03040"
     CDS             complement(14530..15255)
                     /locus_tag="VFD89_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZJ57196.1"
                     /translation="MSKPLIGITCDYDMEKKISQVYIGYYQAILQSGGLPFLIPRIDE
                     GDIQEVLGLLGGVVFTGGGDVDPAYFGEPPNPRLGTINPYRDSLEIPLCREAMGRDIP
                     VLAICRGIQLMNIAMGGSIYQDLESQWDLGPLQKHTQLAPEWYGSHAVELTKGSRLAG
                     LLKSGRLNTNSFHHQAIKDPADCFIVTARCDDGVVEGIESPFHMFAVGVQWHPERMWE
                     KDGRMLCLFKGMVEASKNRSLGR"
     gene            complement(15361..16212)
                     /locus_tag="VFD89_03045"
     CDS             complement(15361..16212)
                     /locus_tag="VFD89_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase"
                     /protein_id="HZJ57197.1"
                     /translation="MSIINKQVTHEIFGKGNVVKYDDAYIKIKFASGDKKFVFPDAFE
                     SYLTLVDKRAANLVNKKLEEQEKERREEALRLKEQEALERKKRLLLEQEQRLRDRKIH
                     PSSQSVFWCRPEEQEKIFAEWEVFAGAIKSGERKGEPRRYARMTQNSACLITRRDTDM
                     KEEDRRITGIFMADRGFDGRKCEDGYIPAHPNYRIHLSEAESKRMLFWNYYSDERYPQ
                     RMIWNSGRQRYFKNVLTAQILRDIVSLRKEQKDEESGQHFLEYFCRHNLIDIEDLPEP
                     SGTLMRG"
     gene            complement(16411..17742)
                     /gene="glnA"
                     /locus_tag="VFD89_03050"
     CDS             complement(16411..17742)
                     /gene="glnA"
                     /locus_tag="VFD89_03050"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HZJ57198.1"
                     /translation="MSKYTREDIKRICKEEKVKFIRLQFTDIFGTMKNVAITVQQLDK
                     ALNNQMMFDGSSIEGFVRIEESDMYLYPDPDSFVIFPWSGGEEKAARLICDVYNADGT
                     PFEGCPRNTLKRALKEAGDMGYRMCVGPEAEFFLFHIDEEGKPTTNTHDNAGYFDLGP
                     VDLGESARQEMCLTLEQMGFEIEASHHELAPGQHEIDFKYDDALATADNVMTFKMVVR
                     IIAQRHGLHATFMPKPVYGIAGSGMHLNQSLFSGEDNSFFDPNGEMELSDAAMYYIGG
                     LMKHAPAITAITNPLVNSYKRLVSGYEAPVYIAWSGQNRSPLIRIPANRGIGTRVELR
                     SPDPACNPYLALAVALKAGLEGIKNRVLPPDPVNCNIYDMTEQERLDGHIPRLPSNLS
                     NAIRALKEDEVIQQALGQHIYSRYIEAKKIEWDEYKIQVTPWELKRYLSKF"
     gene            complement(18003..19736)
                     /locus_tag="VFD89_03055"
     CDS             complement(18003..19736)
                     /locus_tag="VFD89_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HZJ57199.1"
                     /translation="MTKIPRPEYPRPQFVRENSWINLNGEWDFSFEEDIFDRKITVPY
                     AYQTKLSGINIQDFHDIVWYRKNFDLPKQTEGQRIILHFGAVDYESDVWVNGQHAVNH
                     IGGHIGFSVEITDLIRDGKNEIILRAKDDTLDLEMPRGKQYWKERSEGIFYTRTTGIW
                     QTVWIEAVPHSYLKRVWMTPDLDDSALDIEYEIEGSIRDKLLKAIIGYKGIKIAEYGL
                     QEVRNRGIFRAAIDRQTLLEAGLHEDMQWTPENPRLFDINFYICEDDKVIDEVSSYFG
                     MRKVSIVDGKFMLNNRPYYQKLVLDQGYWGDSLLTAPDDGDFVKDIHLAKEMGFNGAR
                     KHQKIEDPRYFYWADKLGFLVWGEMAANYVYSRRAVKRLTHEWMGEIFRDYNHPSIVA
                     WTPINESWGVPNILNKRDEQSLSAALVYLTKSLDQSRPVISNDGWEHTCTDLLTIHDY
                     CPDKGVLMDRYSTLESILDCMPAGKRMFAQGWEYQGQPILLTEFGGISYRTGDWEGWG
                     YSNASSGEDYAKRYYEVVSAVLESRLIQGFCYTQLTDIEQEINGLLTYDRKPKIDIDI
                     IRQINEGKLKL"
     gene            complement(19792..20952)
                     /gene="nagA"
                     /locus_tag="VFD89_03060"
     CDS             complement(19792..20952)
                     /gene="nagA"
                     /locus_tag="VFD89_03060"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HZJ57200.1"
                     /translation="MIILKNGRYLSAEGKFVKGDIHIEDGKIKSINEQDGSLDSGSVG
                     TGAHIYDLEDKRVIPGLIDMHTHGAIGYDVSIATPEEICQLTRYYSDRGITSFFPTTI
                     TAARGDIKSALRNIKAASKMDCGGASIEGVHIEGPFINAAKKGAHDPNWITTPKKSDY
                     DEYREIMGDLRIHLTVAPEIEGGLEFIDYVVKNDATVGIGHTDTDYNRALEGIRAGAS
                     IFTHLFNAMVGIHHRNPGTVGAALTGDAFVEIIGDGMHIHPAVVNIAINAKGPKGVVL
                     VTDSMHAAGLDWGEYDFGGFKILIEGGVAKHEDGTLAGSIISLMDAVKNAMEFSGVSL
                     EDAVAMATINPARVLGLDDKLGSIELGKRGDLVVLDEDLKAHMVFCRGERVK"
     gene            complement(20988..22394)
                     /locus_tag="VFD89_03065"
     CDS             complement(20988..22394)
                     /locus_tag="VFD89_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ57201.1"
                     /translation="MKLGMRTKLIIAFVLVVIGPIANTFIMMAMAITKLNHDPEIVKF
                     NSLNKNFQRVISNVEENFKIIDDYDAFDGILRPILEEFDGSVRVMDATGDLLYDSRGS
                     KDGLMDDSLGADAGLFSLTRYSYSTEIENGDEIAGKIIIDYDINLMPTQIARKVSYHF
                     RLSYILGLGSIFLFISLFTRIISRSVLVPLNELNSATRSIAKGDLDFVIKYKRNNELG
                     RFCQAFDMMRDKLKASIDQRIASERRQKEMIGVISHDLRTPISSIKGYVEALQDGMAH
                     DEEQFQRYLKVIHDKTHRLDALIDDLFQFSQMELGNLKMNLEIMDSSVMLEGIINSVS
                     VDWRGGTTETVIKRPFPSVQIRVDAKRIAQVMDNIMQNAIKYGGDFGVIEVMAAVVDD
                     HLVISVSDNGIGIAVGDLPHIFELFYRGEKSRSRNYGGTGLGLTICKNIVEQHGGKIW
                     AESVLGEGTTISFTLPMV"
     gene            complement(22391..23098)
                     /locus_tag="VFD89_03070"
     CDS             complement(22391..23098)
                     /locus_tag="VFD89_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006636542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZJ57202.1"
                     /translation="MCRRILVVDDEKEISELVRDYLEAAGYQVILAFDGHGALRCWKD
                     HRPHMAILDIMLPMIDGMEVCKRIRMESNIPILMLSAKKSEEDKIIGLDLGADDYITK
                     PFSPKELVARVNAHFRRISQIAKHGNAQKLEDPLIFKDLVIDFKGHGLRVGDKEILLS
                     AKELEVLKFFTLNPNKVLTREEIYDYVWGYQEYGDINTVAVHIRRLREKMEEDPSKPR
                     FIQTVWGVGYKFAGEQP"
     gene            complement(23234..24568)
                     /locus_tag="VFD89_03075"
     CDS             complement(23234..24568)
                     /locus_tag="VFD89_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HZJ57203.1"
                     /translation="MIKGRIGTLKSIMTKWLQGLLELITFFPILLTVGILLIPKEIWI
                     WMVDLSAIYLIGLILGGYLLKKPRYIHFFVGILINSLSAFLLFGNLYAMILFFLSGMV
                     IFDRGVRFRGTKWGDMFPNMALWIGLTLYLLGGSIYSLTPALKPYFKLLAWTGFVYTI
                     IVLFVINTEQLKTASQPVDGTVPIFSSTVLRNNRILILLSIALVMFISYFNDLKDGCV
                     WLARKVGQGFLAIILFLPNLLGTPGPGMGGLPGQGPMEIIPDIEATEPSMFAVVLEKA
                     LMFLAAAAFLVLLWFALRVVFRLLKKAYRRLLEFLKGFVLFQESGGFIDEKERLIGLK
                     DIGRDYRNKFQDWLKGILEKEPKWDELQNNHQRIRFLYRNLLLRCLSGGYTHKNYLTP
                     RETAADILDWDEKESPEIADLSQAYDETRYGEKKIEDSRVRGLAEKFLRKQR"
     gene            complement(24561..25667)
                     /locus_tag="VFD89_03080"
     CDS             complement(24561..25667)
                     /locus_tag="VFD89_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZJ57204.1"
                     /translation="MSIQWIIFVTLIVVSVQAWITDRFMLRGIGYNRYFNVNSAFEGQ
                     EIEMVERISNRKLLPVPWLRIESKISENLEFQRQFNLDIKHQQFHKSLFSLMPYTAIN
                     RRHQIRCRKRGCYRLNSAALTCGDLFGFRDVSRVFELDAELMVYPRLMRLDEIPFLSR
                     SWMGDVVVKRWIVDDPFMYSGVREYSAGDPLNDINWKATARTGRLQVHKKDYTADPRI
                     MVYLNMDISEDMWGPITEPQRIEKGVSYAASIVRHAISQGIDTGFGSNGYTIDEPGLP
                     IRVFPRNGEEHLLFLFETMAKLVIDRGVTFHTFLEEDIKERSSGTFSGSIDYLFITSF
                     VTERMKSQIKQLEDMGNSVEILRLRDPEGGVGND"
     gene            complement(25664..26638)
                     /locus_tag="VFD89_03085"
     CDS             complement(25664..26638)
                     /locus_tag="VFD89_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010278382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZJ57205.1"
                     /translation="MELDKIRSMAQRIKDNIKKVIVGKDEVIDQLLIALISSGHVLLE
                     DVPGTGKTLLAKSLSKSLDCTFKRVQFTPDLLPSDLSGINFYNQKTGEFEFRPGPIFT
                     NILLGDEINRATPRTQSSLLESMEEKQVTVDGETRSLERPFMVLATQNPVETQGTFPL
                     PEAQLDRFLLKVDMGYPSTDEGVEILKRFKERDPFIDIGPVAQSDEIIAAQHSYARVF
                     VSDDILRYIVRIVEVTRQHGDIMLGVSPRGSQALLKAAQVYAILQGRDYVSPDDIKKI
                     AKPVLGHRIILRNVLGMREGQLDDIIHQIFKAVPVPSEKIPSKDEAVL"
BASE COUNT         6439 a         7590 c         5288 g         7851 t
ORIGIN      
        1 tctgaacctc ccatgactaa agtcacgggg ttctagagtt cataagaact catttacgtt
       61 catagcagct aagctacttt gatacgtaca ggcgtgtcca gttcgcctct accgtatagc
      121 cctgttaagg gcacaacgct acttttatag gattctccaa ttcatctcac gacttaagtc
      181 acgagtgttc ttgaggagcc taataaatca ttcttatcta aagtcatgca tcaccaaata
      241 ataaacggtc aacggatatt tcaaaaggat aaattcatgc tccccttttc gggcagccgg
      301 acaacacgta ctttgaaatt ccactgctga atgtttcacg ataaatagcc catatcgcat
      361 tttacataac gcaaaacatt ctccttggtc tacatattac agaaaacaca ttaaccccgt
      421 acaaaatagc tgaatttaac cgatttcaca atggcgggcc gcctccgtaa catatataat
      481 gtatgatgta taattgtaaa ttatatatgt atactatcat aatgtcaatt gaatttccgc
      541 tatttatgaa ctttactcca tgtaagtttc ttccacaaaa ggcttatggg cgcaaacctt
      601 gcaatatttt gtctaccctt gtagattcct tgacaccggc tgtattgctc tgttgatgat
      661 ggagtttctc gccataaaaa tcccgggttc cccacccgac ggttggacac ctgatcagac
      721 ggaaaacccg gaacaatcta tatattattc tgttgatatc tccacccggt acagcgcatc
      781 aggttaatat agccccttta tccgcagagc ttaccattct tgcatatctg gccaagtaac
      841 cattctttat ctttggctcg ggtgcattcc actgctccaa tctggattta aggtcttcgg
      901 cgctgagtct gacttccaat aaaccccctg ggatgttgat gctgattata tcaccctcgt
      961 gtatggcagc tataggcccg ccttccatgg cttcggggga tatatgacct atggatgcgc
     1021 cccgagtggc accagagaat cttccgtcgg tcaaaagcgc cacatcctta tccaaaccca
     1081 ttcccgctat ggcggaagta ggcgagagca tctccttcat acccggcccg cccttgggtc
     1141 cctcgtagcg gattactata acatcaccct tttggatctt cctgcccaag atagcggcaa
     1201 aggcatcctg ttccgaatcg aaaaccctgg cagggccctc gtgtaccatc atttccggtg
     1261 ccacggcaga tcgctttacc accgccccgt caggtgccaa atttcccctc aatacggcta
     1321 tgcccccagt cttgctatgg ggatcctcta tgggacggat cacatcataa tccaaaacct
     1381 gcgcatcttc tatattggct cccatcttat ggcccgtcac cgtcatcaga tccagatcca
     1441 tgaggtcctc ttttgaaagt tcctttatca cagcctgaac gccgcctgcg gcatatagat
     1501 cctctatgcg atgctccccg gcaggactca gcttgcatag attcggggtt ctcatactta
     1561 cttcgttgat gatatccagg ttcatcttaa cccccgcctc atgggctatg gccgttaaat
     1621 gcagcactga attggtagag catccaagtg ccatgtccac cgtaagggca ttctgaaatg
     1681 cactttccgt catgatatcc ctggggcaga tattcttttc taccagctct aaaatcttca
     1741 ttcccgccct cttagcaagc cttatgcgct gggcatatac cgccgggaca gttccgttac
     1801 ccggcaaccc catccccaat gcctcggtca gacagttcat ggaattggcg gtaaacatac
     1861 cggcgcagga tccgcagctg cggcaggcat cctcttctat ttcatatagc tcttcctccg
     1921 tcatgacccc ggcactgaca gcgcctactc cctcaaatac cgagtttagg tccaggctct
     1981 tcccccgttt ctttcctgcc agcatgggtc ccccgcttat gactatgcag ggtatattca
     2041 gcctggcagc tgccatgagc atggcgggca ctatcttatc gcagttgggt atgagcacca
     2101 atccgtcaaa accatgggcc attgtcatag cctcaatgct gtctgcaatg agttcacgag
     2161 atgccaaaga ataattcatg cccacatggt tcatggctat accgtcgcac accccgatgg
     2221 aagggaactc tatgggggta ccccccgcca tccttattcc gtttttaacc gcatcggcaa
     2281 tagtatctaa atgtatatgc cccggaacta cctcgttctg ggaattaact acccctataa
     2341 ggggcagcgc caactcctca tttgtatatc ccatggcctt aaacagggac ctatggggag
     2401 ccttttcata gccttttttt accctatcgc ttatcataag ttacctccct tatggatacc
     2461 acagcaaaaa atccatatat gtcctatcca attcagtcca ttctattcca ataggttatg
     2521 tccttcagct ttcccttagg aacatggaga atcttgtttt tatcgtttta atactttatg
     2581 cttccccgca tttatttttc ccctgatctt tcccatgccc ttaaaaaggg atatggattg
     2641 acagcctttt ctccggaacc ggtccagccg tcccgttcat acatgccaaa atgtaaatgc
     2701 ggagcgaact gcccggtagt gccttccttc ccataaccgg tatccccggc atagcctatg
     2761 atctgacctt tattaaccct atgtcccttc tccagtttat cctcgtatcg ggacaggtgg
     2821 gcataatagt aataaactcc gtcattaccc cttattccta tgcgccagcc ccccagttcc
     2881 agccagcctt ttttctctat cacaccgtcg cataccgcca ggaggggggt tccttcatcg
     2941 cacatcagat ctattccttc gtggcggcgc tccccgccat agcttcttcc gtcgccataa
     3001 ccgtcttcat agtgataatc atggccggat atggggaaga ccttatcatc atagacaccg
     3061 attatatatt ccaggcattt tatctcattc tttaccctac ctatgaactt cccatcgtta
     3121 ttatagtcct tgagcttctt tgacagttca ttcggagagg cgatgccccg caggtgaagg
     3181 gcaatttgtt ccgatctacc cgggcttacc tcctctttgg gtatctcctc agccttatct
     3241 atggctaaca ggtaatacca gggcacacct tgagcatccc ccccatcgaa gtagaccccc
     3301 agatgttcgc tgtcaaattc cgcgatccta tatttagtat aaaagccctg ctcctgaacc
     3361 ctgttgagga tcactaaaat tactaatata ataaacagag ccaaaagggg ataaaatctt
     3421 ttcccgttta ttctatatct tcttcgcgat cccaccctat acctgacggt attcataatt
     3481 gtcaccaccc cggatactat atatggcttt aacgctacag gcctccagcc tatagtacat
     3541 agtctattat ctttcggatg gatttacaac ggtatagggt taaattaacc tattatactg
     3601 cccataatat cgttgtagga taggggtaaa cccgtaaagt tatatctccc gggcaataag
     3661 gtcccccatc aaatcggtac cgatctcctt catcccgggc tgcatgatat ccccggtcct
     3721 atatcccata tccagtaccg acgagacggc tctttctata tccctagccc cctcttccac
     3781 atccccgcta tacctaagca tcatagcgac gctcatgatg gtggctatgg gattcgcctt
     3841 atctcggccc gcgatatccg gagccgaacc gtggattggc tcatagatac cgggattttt
     3901 atccccgaga cttgcagaag gcagcatacc caaagagccg gtaagcatgg atgcttcatc
     3961 gcttaaaata tccccaaaca tgttttccgt gactattaca tcgaattgct tcgggtttcg
     4021 gataagctgc atggcggcgt tatccacgta catatggctc agctctattt ccggataatc
     4081 cgaggcaatc gcccccacaa cctctctcca cagcctggag ctctccagaa cattggcctt
     4141 atctacagac ataagctttt tattccgtct ttctgccagc tcaaatgcca ccctggctat
     4201 cctttctatc tcagatacac tatattccat ggtatcccaa gcttttgcgg aatcaccctg
     4261 tcctgcccgt cccctttcgc caaagtacag gccgccggta agctcgcgaa ccaccatgat
     4321 atccagcccg tctcccagta cttccgcctt aagggaggaa gcctccttta gctggggaaa
     4381 taatatggca ggccggaggt tggcaaatac ccctaaagcc ttcctgatgc caaggagccc
     4441 ggcctcggga cggaggtgtc cggggaggct atcccacttc tctcccccca ctgcacctag
     4501 gagcactgca tcgctgtccc tgcacacctt tacggtttcc tcaggaaggg gcaccccggt
     4561 tttgtctata gccgcccccc ccatgagagt ttccacaaag gtaaattcaa cgtcatattt
     4621 acgggagacc gcatccaacg ccttaagccc ctgcgctatg atctccggac caattccgtc
     4681 ccccggaatt aaggcaattc tcatggctat tccccctttt ccttcacata gttaataagc
     4741 ccgccggccc tcattatatc ctgcataaac cccggaaagg gggtcccttg ataggtccta
     4801 ttcttagtga tattggttat aacaccgcta tagacatcga tggatacctg atccccgtcc
     4861 tctatgtcct tggccgcctc agggctttcc attatcggga gtccgatgtt gatggcgttt
     4921 cggaaaaata tccttgcaaa ggtctcggct atgacgcagg agataccaga ggcctttatc
     4981 accccggggg cgtgctccct ggaggatccg ctaccaaaat tcttacccgc caccataata
     5041 tccccgggct ttaccttacc tacaaaatcc ttatccaaat cctccatgca atgcttagcc
     5101 aattccttca tgtcgctgat attgaggtat ctggcaggga tgattacatc ggtatccaca
     5161 ttatccccgt actttattac agttcctttc atgattcaac tacctcctcc ggggaagaga
     5221 tcttcccggt tatcgctgat gccgccgcta tcgcagggct cgctagatag acttcactgt
     5281 ccggatgtcc cattctgcct acaaagtttc tgttggtagt agccacagcc cgttcccctg
     5341 cagccagcac tcccatatgc ccgccgagac aaggcccgca ggtgggggtg cttacagcgg
     5401 ccccggcctc tacgaatatt tcaagcagac cctcttgcat agcctgcaga taaatctttt
     5461 gggtggctgg gaagattatt gttcttacac ccttcttgac cctccgtccc tcaagtatct
     5521 tggccgctac cctcaaatct cctatcctgc cgttggtgca ggatcctatt accacctgat
     5581 ggatgggtac atcccccgct tcatcgatgg ttcgggtgtt atccggcaga tgggggaacg
     5641 ctacggtggg ccttagggct gatagatcca cctctataac cctggtatac tcggcatcct
     5701 cgtcggcctt atagaccgta tattccttgg cggaatgctc cttcatatag gctatggtct
     5761 tatcatcgat ctcaaagatg ccgtttttcg ctccggcttc gattgccata ttagccatgg
     5821 taaacctatc gtccatggat aggctggcga cccccggtcc tgtatactcc atggactcgt
     5881 agagggcgcc gtctacccct atcatgccga ttatatggag aattacatcc tttccgctga
     5941 cccatttagg caggcttccc gtaaggacta tctttatggc ctccggaacc ttgaaccagg
     6001 cccggccatc ggccataccg gcagccatat cggtgctgcc tactccggtg gaaaatgcgc
     6061 ccaatgcccc gtaggtacag gtatgggaat cagccccgat taccacgtcg ccgcctacca
     6121 ccagcccctt ttctgggatg agggcatgct caatccccat ctcgcctatc tcgaaatagt
     6181 tttctatttc cttgtcccca gcaaattcgc ggatgaattt ggcctgctcc gccgacttta
     6241 tatccttgtt gggggtaaag tggtccggca ctattgcgac tttgctcttg tcgaacactt
     6301 cgcctacccc aatcttatta aactccttga tagctacagg gcttgttata tcgtttccca
     6361 gcaccaaatc caagttggcc atgatgagct gaccagcctc taccctgtca aggcctgcat
     6421 gggcggccag tatcttttgt gtcatggtca ttcccatagt ccccactcct tttcattaat
     6481 aatataaaaa cgtcgggtta gttattgccc ataaccatta ccgatgtcct cgccttcggc
     6541 atttccgttt agataaagaa agtactctat agaatccacc agtgcatccc agctggcctc
     6601 tattatattg gtagacaaac ccaccgtacc ccatacctta cgtccatccg tggattctat
     6661 atggacccta accttggcag ccgtggcttt ggtggtatcc agcacccgga ccttataatc
     6721 cgtcaggcgc atcctcttaa gctggggata aaatatttcc aaggctttac gaagggcctt
     6781 atccaaggca ttgaccgggc catcaccctc agctgctata atctcttcgc tgccgtctac
     6841 ctttaccttc acgatggccg aggcgctata atcgttttgc caatgctcct cgcacagcac
     6901 cctaaaatcc ttcacctcga aaaaggtttt ggccttcccg agggcccgtt ttactaccaa
     6961 ttcaaaagag ctttccgccc cttcgaactg atagccgtca tgctccaatt gcttcagcct
     7021 atccataact aggatggttt cctctgagtc tttatttacc cagggcgcta cgtcctgaat
     7081 cctttttaag atggtgctcc ttccggaaac ctcggacatc aggatcctcc gctcgttccc
     7141 cacaagttcc ggcgctatat gttcaaagga atgttggttt ttcatgaccc cgtctatgtg
     7201 cataccgccc ttgtgagcga aagcgctgtt ccccacatag ggttgtcttt cgttataggg
     7261 gaggtttgcc agttcactta tatagcggca ggcggaactg agctccttga gtccctcgtc
     7321 ctttatacag gttttatccc tctttagctg gaggttgggt atgataacgg agaggttggc
     7381 attaccgcag cgctccccat atccattgaa ggtcccctgg acctgcacta caccctgctc
     7441 caccgccatg atggaattcg cttccgccat accaccatca ttatggcaat ggataccgat
     7501 gggtatggtg aattttttta ggacctcact tgttatttca gtgatctcca agggaaagca
     7561 tcccccgttg gtatcgcaga gcaccaacca gtccgcccct gccttcgccg ccgcttccaa
     7621 tgcccccatg gcatactcac tattggcctt atatccgtca taaaagtgct cggcatcgaa
     7681 gatcacctct ttaccatggc ttttgaggaa ggaaacggta tcatatatca tattaagatt
     7741 ctcttttaga gatgtcctga taatctccgt gacatgaaaa tcccaactct ttccgaatat
     7801 cgccacggct ggtgtgtccg ccgttaacag ggccttgaca ttggcatcgt cttgggcctt
     7861 tatacccggt cttcgggtgc tcccgaaagc cgcaagtctg gcatgcttta ggggcctttt
     7921 tttcatcctt tggaaaaatt ctaaatcctt ggggttcgag cccgggttcc ccgcctcaat
     7981 ataatccacc cccaggctgt caagccttct ggcaatccga agcttatcat ttacggtaaa
     8041 ggatattccc tccgcctgtg ccccgtccct cagggtagta tcatagatga aaaccttttc
     8101 tttattcatt aaattcccaa ttattattca ctccatttca gtttcataaa gcatcttgtt
     8161 gatggcattt acgtatccct tggcgctggc ctcaataaca tcggtgctga tcccccggcc
     8221 cacaaagata tcaccgtttc tgctcacctt tacggttacc tccccaagag catccttacc
     8281 gctggtaacg gctctcagat aatagtcctt aagattcacc tcgatacctg cggctctgtc
     8341 aatggccctg aaggtagcgt ctatggggcc gtcgccgcaa gccgcctcct ggatgagccc
     8401 gtcatccacc ttaagcctca ccgtagaggt ggatatggtg gagtttccgc tggatatatg
     8461 gaagtattcg agttcatata cggcgggtac ctgtatgacc ttttcgctga ggagagcctc
     8521 aatatcccgg tccgtaacga tttttttctt atccgctagg tccttgaact tgctgaaggc
     8581 ctcgttgatc ttttcggtat ctaggtgggt ataacccatg tccccaagct tttcttcaaa
     8641 ggcgtggcgc cccgagtgtt taccgagcac catctcgctc tgcctaaatc cgacggattc
     8701 cggggtcata atctcatagg tctccctgtt gctgagcatg ccgtgctgat ggattccgga
     8761 ttcatgggca aaagcattgg ctcctatgat tgccttgttg ggctgtatct gaacgcctgt
     8821 gagatggctc accaagcgac tagtcctata gagttcctcg gtttcgatcc cggtttcaca
     8881 tccataaaaa cccttccgtg tcttaagggc cataacgatc tcctccaagg cggcatttcc
     8941 ggctctctcg cccaagccat ttatagtgca ttccacttgg gtggccccat tctcaatggc
     9001 tgccagggaa ttggaaaccc ccattcccaa atcattatga caatgaacgc tgagtacgac
     9061 atcctctata cccttaacgt tttccataat ggatgcgatg agttccccaa attcttgggg
     9121 cgttccatat cctacggtat ccgggatgtt tatagtggtt gcaccggccc ctatagccgt
     9181 ttctattacc ctgtataaaa actccggtcg ggaccttgaa ccgtcttctg cagagaattc
     9241 cacatcctcg caaagggact tggcgaaggt taccgcatct ttcacctgct taagtacctc
     9301 ttccggtttc atcttcagct tatattccat atggatatcc gaggtggcta taaaggtatg
     9361 aatccgcggg cgagccgcat actttaaagc ctcccatgcc ctttcgatgt cccccttcac
     9421 cgcccgagcc aaccccacta taatgggctt tctgatgctt tgagctatgg cctttaccgc
     9481 ttcaaagtcc cccttagatg ctacgggaaa acccgcctct atagcatcca cccctaagcg
     9541 ctccagttga gccgctatct ccactttctc atgaacgtta aggctgatac ccggtgattg
     9601 ctcaccatcc ctcaaagtgg tatcgaatat atagaccctt ttggtcattg atctctcccc
     9661 ctaatcttta tttctcttca atccagggca tcatctttct gagctctttt ccaacccttt
     9721 cgatgatatg gtcctgttcc ttctttctgg tagcctttaa ggagacttga ttggcttggt
     9781 tctccaggat ccagttttta gcgaaggttc cgtcctgaat ttcccctaaa acatttttca
     9841 tctcctcacg ggtgtcttcg gtgatgatcc gtcttcctac ggaataatcc ccgtattccg
     9901 ctgtattgct tatggaatac ctcataaagg acaggccccc ctgattcatg aggtccacta
     9961 tcagcttcat ttcatggaga cattcaaaat aggcgctttc aggctgatat cccgcctcta
    10021 ccaaggtttc gaatccggct tttataagct ctgagatacc gccgcacagg accgcctgct
    10081 ctccgaacag gtccgtctcc gtctcctctt tgaaggtggt ctcaagaacc ccggccctgg
    10141 ttcccccgat acccttggcg taggccaggg caaagtcctt ggctctgccg gagtaatcct
    10201 gatgcacggc tacgaggcag gggactcctt ttccttcctg gtattggctg cgcacggtgt
    10261 gccccggtcc tttaggggcc accataaata cgtccacgtt atcgggcggt atgatctgtc
    10321 cgaagtggat attgaagccg tgggcaaaga taagtccgtc accctctttt agattaggtt
    10381 caatgctttc cttgtatatt ccggcctggg tctcgtccgg ggtgagtatc attatatagt
    10441 ccgcttcacc cgccgcctca cccgcctcaa gtaccctaag cccggccttt tccgcctctt
    10501 cccaggatct gctccccttg tataatccta ccctgacatc gaccccgctc tcctttaggt
    10561 ttagggcgtg ggcatggccc tggcttccat agcctattac ggctaccctt ttgcctgcca
    10621 ataattccat attcgcatct tgatcatagt acattattgc cattttcatg tcctccttta
    10681 ttcttatccc catattttaa catccttttg cctcggttta tggcgatagc tccggtgcgc
    10741 accatctcct ctatgccata gtcctttagc atgtcctcca gggcagtaat tttttcgctg
    10801 tccccggtta tctccacggt gagatggttt tttgtaatat ccactacctg ggccctaaat
    10861 atgttaatta tttcgacgat ctcatgccta gtcttaggat tagccttgac cttaatcaga
    10921 gccagttccc tagtcaccga atcgtttcca atatcggata cctttataac atcgattaac
    10981 ttgttaagct gtttggttac ctgctctaca atataggagt ctccctctac gacgatggta
    11041 atcctggaga cggtagcatc ctccgtcatg cccacagcca ggctgtagat gttaaatccc
    11101 cttctgctga aaagtccgga aaccttagat aaaacccccg ggtggttttc aaccaatacc
    11161 cctaatatgt gcttcatcat ctttccctcc taagatggga attacattag ccgtgggatc
    11221 tataacaaat tctccgataa taggtccgtc ccaattgagc agctttctaa ttccccattc
    11281 gattctttct tggctttcta ccctaaatcc cttaatcccg taggcctgag ccagttttat
    11341 aaaatccggg ttaccttcca tacgggtttg gtagtatctt gcttcatgga tttcctcctg
    11401 gatttccctg accataccca agtaaccgtt gttgagtatc attatcttga ccccggctcc
    11461 ctcctgcacc aatgtcgcca gttccgccaa gttcatctgt aggcccccgt cacctatgat
    11521 aaggaccact tcactgtccc tacgcccaag cttggccccc agggccgccg gcagcccgta
    11581 tcccatggta cccagtcctc cggaagtaag aaaacccctg ggctttctgg tcttatagta
    11641 tcttcccgcc catatctggt gctgccctac gtccgttacc aatacggtgt tttccgggac
    11701 catacccgag agctttttaa ttacttccat agggttgagt cccttcaggc aatcccctac
    11761 accctcatgt ttttgtttcc atgccctgac tgtggatagc cagccccaat catgtctgtc
    11821 aatatccttt tttagcagct ctcccaaggt ggtctttata tcccccacga tgggaatatt
    11881 tacgcctata ttcttgccta tctccgccgg gtcgatatct acatggataa cctttgccct
    11941 ttgggcgaac tcttgggtct tacccgtagc cctatccccc atcctggcgc ccagcaaaat
    12001 cagaagatcc gcttcttcca ccgccctatt agctgcataa atgccgtgtt ggcccaagaa
    12061 tcctaaggat agacggtgat cccccggaaa ggcccctatg cccatcagag tggatgctac
    12121 cggcgcctta attctctccg ccagctccat gatctcggca gttgcatcgc tggaaacagc
    12181 gccgccgccc acacatataa ggggcctacg gctctcctcg atagccttga ccatcctctt
    12241 tacctgtccg ggatgtcccc tgtaggtcgg cttatatccc cttaaattta cttcgtcggg
    12301 gtattcaaaa tccaaagggg ccatggctat atccttgggt atatcgatta ggaccgggcc
    12361 gggccgtccc gtggaggcta tgtaaaaggc ctccttgaaa attcgtggaa ggtcctccgg
    12421 attttttacc aggtagctat actttgtaaa gggctccgta gcccctgtta tgtccgcttc
    12481 ctgaaacaca tccctgccta ccatagacag cggcacctgc ccggtaatag ccactattgg
    12541 aatggaatcc atatacgccg tggcaatccc cgttatcata ttggtggccc ctggacctga
    12601 ggtggatata cacaccccca cctttcccgt agccctggca taaccgctgg ccccatgggc
    12661 tgccgcctgc tcgtttcgaa ccagtacgtg atgaattttg gatctgctga acgcatcata
    12721 gatatgggtt acggctccac cggggtatcc gaaaattata ttcacccctt ccaactccaa
    12781 ggccctaact accgcttctg cacctgtcat ttgcatatgt cttgcacctc ctaccatact
    12841 acgaaataaa aaaaccttcg cccctacatt tgcagggacg aagggttacc ttcgcgttac
    12901 cacccggctt tatctgtacc tcacggcaca ggccttcaca ggttctatca aaccccggcc
    12961 tgtaacgtga cctcacgtca aaccctaatc gagatccaat ctctttcaag tctgcttctc
    13021 aggagtgagc attcaatcca attgcccgcc ggttcgcact aaccaccggc tcactgaaga
    13081 tggccttagg ggatcttcta tctccgtcat agaatttatt taataatatc caagatgtcg
    13141 tcgtcgagtc tataaaaaaa gtataagaat gcccgacaac agacctcttc ttcagaagaa
    13201 acctgccgcc gggtcttttt tacccacggt gtaatgctaa atgttagcat cttgtatcgc
    13261 tcggtcacag tcccgtaagg tacggcggaa ccctagatac acttccttct ggctactccg
    13321 atttttagat ttgctaatta tcatagcatc cccagaaact cttgtcaata gccttttttt
    13381 ctttttagca gttattcctg tcctttttgc tcaataaccc tttttaccct cttttcaaag
    13441 ctctccctat ccaacatgac aatgcgcccg caccttaagc acttcatctt catatctgca
    13501 cccacccggg tgacctccca ttgactgttt ccacagggat gctttttctt tgtctctata
    13561 atgtccccta cataaaactg tcttgtcaat actatgcctc ctcccattag cccttcccta
    13621 atccctattg attactacca gcctgggata tggaatctcg attttgtttt cctccaaggc
    13681 gacttttata gcctttctga gatcccgctc taccccccaa tgcttcatag gcaacgttct
    13741 acccaccacc ctgagggtaa gccctatgct atccatctct acgataccga cagcccttgg
    13801 gatctccacg atatccgtgt ttgccgcagc gtacttctct atcactttat ctataaccgc
    13861 gatagcccta tcaatatcgg cctcatatgc tatggtcacg tccactatca ccgcagaatt
    13921 ccccctggaa tagtttgtga ccataaatat ttgtccattt gggataatgt ttatatcccc
    13981 cttaaaccct ctaatcttgg taatcctgag ccctatttcc tccacggtcc cggttatatc
    14041 ctcgatcttt ataaaatccc ctacgctgta ttgatcttca aatattataa aaaaaccggt
    14101 tataacatct ctgaccagac tctgagcgcc aaatccgata gccaagcccc ctatgcccgc
    14161 agtcgctata atggatgtgg tctcaatgcc tatttcgtcc atgatcatca aaaacccgat
    14221 gaagtaaatt acgtatctca agatactctg gaccaacgcc ctgagggtgt cattcttctt
    14281 tttattaaca tggaatctgc ccttggcccg gctctcaaag aacccgttta ttaagaatct
    14341 tccgagcctt ataaaaaaaa cagctgccgc ccatatgagg attaccctaa gtatcctata
    14401 gcctatggct aagtaatggt cgacagtcca ttggtttaac caagcgatga tatcatctag
    14461 cataccttta gaacactccc tcccgctaca tatccgttcc cgagacgaca ggtattctgc
    14521 tccaagaccc tagcgtccaa gactcctatt ctttgacgcc tctaccatcc ccttgaacag
    14581 gcacagcatt ctcccatcct tttcccacat cctctcagga tgccattgta cacccacggc
    14641 gaacatatga aaaggcgatt ctattccttc aactacaccg tcatcgcacc ttgccgttac
    14701 gataaaacaa tctgccggat cctttatagc ttggtgatgg aaactattgg tgttaagcct
    14761 tccactcttg aggagccccg ccagcctcga gcccttggtt agctccactg catgacttcc
    14821 gtaccattca ggagcgagct gggtatgctt ttggagaggt ccaaggtccc attggctttc
    14881 caaatcctga tatatgcttc cccccatggc gatattcata agctgtatcc cacggcaaat
    14941 tgccaatacc ggtatgtcac ggcccatagc ttccctacac aacgggatct ccagactgtc
    15001 cctgtagggg tttatagtcc caagcctcgg atttgggggt tcaccaaaat atgcgggatc
    15061 tacgtctccc ccacctgtaa atacaacccc gcccaataga ccaaggacct cctgtatgtc
    15121 gccctcatcg atccttggaa tcaaaaatgg caacccgccg gattggagta tagcctgata
    15181 gtagccgata taaacttggg agatcttctt ttccatatca taatcgcagg ttatcccaat
    15241 aaggggtttt gacataccca tccccttctt tctatatgat ctcacataca atcccaaaaa
    15301 ttggcgggta aaagtcttac agccgattcg cctagaaata ccttttcttt agaatccgtg
    15361 ttagccccgc attaaggtac cgctgggttc tggcaagtct tctatgtcta ttagattgtg
    15421 tctgcaaaaa tattcaagga agtgctgacc gctttcctca tctttttgtt ctttcctcaa
    15481 tgaaacgata tctcgcagaa tctgtgccgt caatacgttc ttaaagtatc tctgtcttcc
    15541 cgaattccat atcatcctct ggggatacct ttcatctgaa tagtaattcc aaaaaagcat
    15601 tcttttagat tccgcctctg acagatgaat tctgtaattt ggatgggcgg gaatgtatcc
    15661 gtcttcacat tttctgccat cgaaacctct atccgccata aatatgcccg tgatacgcct
    15721 atcctcttcc ttcatatcag tatctctgcg cgttatgagg catgcgctat tttgagtcat
    15781 cctggcatat cgcctgggct ctccctttct ttccccgctc tttatcgctc cggcaaagac
    15841 ctcccactct gcaaatatct tctcttgctc ttcaggcctg caccagaaaa cggattggga
    15901 gctgggatga attttccgat cccgtaggcg ttgctcctgt tcgaggagaa gtcgtttttt
    15961 tcgttccaga gcctcttgtt ctttcagcct taacgcttct tcccttcgtt ccttttcctg
    16021 ctcttccagc ttcttattta ccaaattagc cgctctcttg tctaccaggg ttagatatga
    16081 ttcaaacgca tctgggaaaa caaatttttt gtcccccgat gcaaacttta tcttaatata
    16141 tgcatcatca tatttaacca catttccttt accgaatatc tcatgggtta cctgtttatt
    16201 gattatgctc atcgattaat tcccctctgc tttagtatat agtccgtgtc atctcctgta
    16261 gaacagtaaa atctgatgtc tcgtctaaac agggactatt accattatac cagcaatccc
    16321 gggtttattc aaaattgtct gaaaaagtaa atcaaaaagc agccccaagc gccaataatg
    16381 cgggcacttg caggctgctc catccttcaa tcaaaacttg ctgagatatc ttttcagttc
    16441 ccaaggggtt acctggatct tatattcatc ccattctatc tttttagcct ctatataccg
    16501 gctgtagata tgctgaccca aggcctgttg gatgacctca tcttccttta aggcccgaat
    16561 agcattggac agattgctgg gcaagcgtgg gatatgtccg tccagccttt cctgctctgt
    16621 catatcatag atattacagt tgacgggatc cgggggtagt accctgtttt ttatcccctc
    16681 aagccctgcc tttagtgcga cagccaacgc cagatagggg ttgcatgcgg gatcggggct
    16741 tctcaattcc acccttgtac ctatgccccg atttgccgga atccggataa ggggacttct
    16801 gttctggcct gaccaagcga tgtagaccgg tgcctcatat cctgatacca gccttttata
    16861 ggaattgacc aaggggttgg ttatagctgt aatagcgggg gcgtgtttca taagcccacc
    16921 tatatagtac atggccgcat cgcttaattc catctccccg ttgggatcaa agaatgagtt
    16981 atcttcgccg ctaaataggg attggttaag atgcattcca gacccggcta tcccgtatac
    17041 cggctttggc ataaaggtgg catggagtcc gtggcgttgg gcgattatcc tcaccaccat
    17101 tttaaaggtc attacattat ctgcagtagc cagggcatcg tcgtatttaa agtctatctc
    17161 atgctgtccc ggggctagtt catggtgcga ggcttcaatc tcaaatccca tctgctccaa
    17221 ggtcaaacac atctcctggc gggcgctctc ccccaaatcc acgggtccca aatcgaaata
    17281 tcccgcatta tcgtgggtgt tggtggtagg tttgccttcc tcgtctatat gaaacagaaa
    17341 gaattccgct tccgggccta cgcacatcct ataccccata tcccccgctt cctttaaggc
    17401 cctttttagg gtgtttctgg ggcaaccctc gaagggtgtt ccatccgcat tgtacacgtc
    17461 acagatgagc ctggccgcct tttcttcccc accgctccag ggaaatatta caaaagaatc
    17521 gggatcaggg tatagataca tgtccgattc ctcgatccta acaaatccct ctatagaaga
    17581 accgtcaaac atcatttgat tatttaaggc tttatccaat tgttggactg taatagccac
    17641 attcttcata gttccaaaga tatccgtaaa ttggagtcgg ataaacttca ccttctcctc
    17701 tttgcatatc ctctttatat cttccctagt atatttagac ataaaacaat cctcccttta
    17761 ttatggataa ccctcaattt tctattatgt tcatcaaatc aaaataccgt tttcataaat
    17821 gcatcaaata aaacctacaa ggttttatca caataatcgc atttattata ccataagtta
    17881 tcggggttgg tccaaaaatt aaattaaagg gcgataggcg gactgaatcc ccatcgccct
    17941 ttattgaata tttcaaagga gatgatccgc caataaggca gaccgctaat tatcacaacg
    18001 ggctagagtt ttaatttccc ttcatttatt tgcctaatta tatcaatatc aatcttgggt
    18061 ttacggtcgt aggtcaaaag cccgttgatc tcctgctcta tatccgtcaa ttgggtatag
    18121 caaaacccct gtatgagccg ggattccagc acagcggaaa ctacctcata atacctctta
    18181 gcataatctt ccccggaact agcattggaa tagccccagc cttcccaatc tccggtccta
    18241 taggatatgc ccccaaactc agttagcagg ataggttgcc cttggtattc ccagccctgg
    18301 gcaaacatgc gcttacccgc cggcatacaa tcgagaatgc tctctaaggt gctatacctg
    18361 tccatgagga cccctttatc cgggcaatag tcatggattg tcaagaggtc ggtacaggta
    18421 tgctcccatc cgtcattgga tataacaggc ctgctttgat ctagggactt ggtcaagtac
    18481 accagagctg cgctgaggct ttgctcatcc cttttgttca gtatattcgg tactccccat
    18541 gactcgttga tgggggtcca ggcgactata gagggatgat tatagtccct gaatatttcc
    18601 cccatccatt cgtgggtgag gcgctttacg gcacggcggg aatagacata gttagctgcc
    18661 atctctcccc ataccaaaaa gcccagctta tcagcccaat aaaaatatct aggatcctct
    18721 atcttttggt gcttccttgc accgttaaag cccatttcct tagccagatg aatatcctta
    18781 acaaaatccc cgtcgtccgg tgcggttaag agggaatccc cccaatagcc ctgatccagg
    18841 accaacttct ggtaatacgg cctattattt agcatgaatt taccgtcaac tatggacacc
    18901 ttgcgcatcc caaaatagga actcacttca tctatgacct tatcatcttc gcaaatatag
    18961 aaattaatat caaacaatct ggggttttcc ggggtccatt gcatgtcctc atggagacct
    19021 gcctcaagta aggtctgccg gtctatagca gctctaaaga tccccctgtt cctcacttct
    19081 tgcaggccat attcggctat ctttatgccc ttgtagccta taatggcttt taataattta
    19141 tccctgatgg atccttctat ttcatactcg atatccaagg cgctgtcatc cagatcaggg
    19201 gtcatccata ccctctttaa gtagctatgg ggtaccgcct caatccatac cgtttgccat
    19261 atacccgtgg tcctggtata aaatattccc tcggacctct ctttccaata ttgctttccc
    19321 ctaggcatct ccagatccag tgtatcgtct ttggccctta ggataatttc atttttcccg
    19381 tccctgataa ggtctgttat ctccacacta aatccgatgt gcccgccgat atggttaacg
    19441 gcatgttgac cgttgaccca gacatcactt tcgtaatcaa cagcgccaaa atgaaggata
    19501 atcctttgac cctccgtttg ttttggaagg tcaaagttct tcctgtacca gactatatcg
    19561 tgaaaatcct gtatatttat accggacaat tttgtctgat aggcgtaggg caccgttatc
    19621 ttcctatcaa agatatcttc ttcgaaggaa aaatcccatt ccccgtttag attgatccaa
    19681 ctattttccc ttacaaattg aggtcttgga tattcaggcc tgggaatctt agtcatgttt
    19741 atcacccctg gttttaataa ttgctagaca tatctcaaca gacaataatg attattttac
    19801 ccgctcaccc ctacaaaaca ccatatgcgc ctttaaatcc tcgtctaaca ccaccaaatc
    19861 tcccctcttg ccaagctcaa ttgagcccag cttatcgtca agccccagca ccctggcagg
    19921 atttatggtc gccatggcca cagcatcctc tagggaaaca ccgctaaatt ccatagcgtt
    19981 ctttaccgca tccataaggc tgattatgct ccctgccaat gttccgtctt cgtgtttggc
    20041 cacccccccc tctatcagga tcttaaaccc gccgaaatca tattcccccc aatcgaggcc
    20101 agcagcatgc atagaatccg taaccaggac caccccttta ggccccttcg cgttgattgc
    20161 gatattcacc accgccggat gaatatgcat tccatcgcct atgatctcaa cgaaagcatc
    20221 cccagtcagg gcggcgccta ctgtaccagg gttacgatgg tgtattccta ccattgcgtt
    20281 aaataggtgg gtgaatatgg atgcacctgc cctaatcccc tccaaagccc tattataatc
    20341 ggtatcggta tgcccgattc ctaccgttgc gtcatttttt actacataat ctataaactc
    20401 caggccacct tctatttcgg gagcaacggt caaatggatt ctcagatccc ccattatttc
    20461 gcggtattca tcatagtcag attttttcgg tgtagtaatc caattcgggt catgggcccc
    20521 ttttttcgcc gcattaataa aagggccttc aatgtgcacc ccctcgattg aagcaccacc
    20581 gcaatccatc ttagatgctg ccttgatatt gcgcagggca gactttatat cccctcttgc
    20641 cgcggttata gtagtcggaa aaaaggaggt tatccccctg tcggaatagt atctggtaag
    20701 ctggcagatt tcctcgggag tagcgatgct gacatcatag cctattgccc cgtgggtgtg
    20761 catatcgatg agcccaggaa taaccctttt atcttccaag tcatatatgt gggctcctgt
    20821 ccctacagaa cccgaatcta aagacccatc ctgctcgttg atggatttta ttttcccatc
    20881 ttcgatatgg atatccccct ttacaaactt cccttctgca gatagatatc tcccgttttt
    20941 cagaataatc atgttttccc tccatccata atttatattt ccttctatta caccattgga
    21001 agggtaaagg atatagtggt accctcccct aaaacgctct ctgcccaaat cttaccgcca
    21061 tgttgttcaa caatattttt gcatattgta aggcccaatc cggttcctcc gtaatttctt
    21121 gaccgggatt tttctcctct gtagaatagt tcgaagatat ggggaaggtc tcccacagct
    21181 attcctatgc cgttatcgga gacggatata accagatggt cgtcgaccac agctgccatc
    21241 acctctatta ctccaaaatc tcccccatac ttgatggcat tttgcattat attgtccata
    21301 acctgggcga ttctttttgc atctacccta atctgcaccg acggaaaggg cctttttatc
    21361 actgtctccg tggtcccacc tcgccaatcc acagagacgg aatttattat cccctccagc
    21421 atgacactgc tgtccattat ctctaagttc atcttcaaat tccccaattc catctgggag
    21481 aattgaaaaa gatcgtcaat cagcgcatcc aacctatggg tcttatcatg tatcaccttg
    21541 agataccttt ggaattgctc ctcatcgtgg gccataccgt cctgcagggc ctcgacgtat
    21601 ccttttattg atgagatagg agtacgcaga tcatgggaga ttaccccgat catctctttc
    21661 tgccgtcttt ccgaagcaat ccgctggtct atggatgcct tcagcttgtc acgcatcata
    21721 tcaaaggcct gacagaatcg gcctaattca ttgtttctct tgtattttat aacgaaatcc
    21781 aggtcccctt tggctatact tctggtggcg ctattcaatt cattcaacgg cactaaaacg
    21841 cttctggata taatccgggt aaacaacgat atgaatagaa atatagatcc caagcccagt
    21901 atatagctca atctaaaatg atatgaaacc tttcgggcta tttgggtggg catcaggtta
    21961 atatcataat cgattataat ttttccggct atttcatcgc cgttttctat ttcggtggaa
    22021 taagaatacc tcgtcaggct aaacaaaccg gcatccgccc ctaaagaatc atccattagg
    22081 ccgtcttttg atcccctgga atcatagagg agatctcccg tagcgtccat taccctgaca
    22141 ctaccgtcga attcctctaa aatgggcctt aaaatcccat caaaagcatc gtaatcatct
    22201 attattttga aattctcctc cacattactt ataacccttt ggaagttttt attcaggcta
    22261 ttgaacttga caatctccgg atcatggttc aattttgtta tagccatggc catcataata
    22321 aaggtatttg caatcggtcc tatgaccacc aacacaaaag ctattataag tttggtgcgc
    22381 atccccagct tcacggttgt tcacctgcga atttgtagcc taccccccat acggtttgaa
    22441 tgaacctggg cttcgaggga tcttcctcca tcttttccct caacctcctt atatggacag
    22501 ctacggtatt tatatcgcca tattcctggt atccccatac atagtcatag atttcctccc
    22561 tggtcaggac cttattgggg ttcaaggtaa agaactttag aacctccaat tccttggcag
    22621 acaagaggat ctccttatcc ccaactctta ggccatgccc cttaaaatct attacaagat
    22681 ccttaaatat taatgggtcc tccaatttct gtgcattccc atgcttggcg atttgggata
    22741 tccgtctgaa atgggcattg accctggcca ccaattcctt agggctaaag ggctttgtga
    22801 tataatcatc ggcgcccaag tcaagaccga ttatcttatc ctcctcgctt ttcttagcgc
    22861 tcagcatcag aatgggtata ttagattcca ttcgtatcct tttgcagact tccattccat
    22921 ctatcatggg gagcataatg tctaagatag ccatatgggg cctgtgatct ttccagcatc
    22981 tgagggctcc atggccgtcg aatgccagga ttacctgata tcctgccgcc tctaagtaat
    23041 ccctgaccag ctcgcttatc tccttttcat cgtctacaac taaaatcctc ctgcacatat
    23101 atctccgaga ccccctctgt ctttacttat acccgtcccc gtctgtagac taacccaatg
    23161 tttttccaat ttctgcatca tatatgacca cgggcgtctt ccgcccacct gatccattgg
    23221 gtgatgtttt atgttatctt tgttttctta ggaacttttc ggcaagaccg cggaccctac
    23281 tgtcctctat ctttttttct ccatacctgg tctcgtcata tgcctgggat agatccgcta
    23341 tttcggggct ttccttctcg tcccaatcca ggatatccgc agccgtttct ctgggggtta
    23401 agtagttttt atgggtgtac cctccggaca aacatcttaa cagcagattt ctatataaaa
    23461 accttatccg ctggtgattg ttttgaagtt cgtcccattt tggctccttt tccaagatac
    23521 ccttgagcca atcctgaaat ttattccggt aatccctgcc aatatcctta agtcctatga
    23581 gtctttcctt ttcatcgata aatccgccgc tttcctgaaa caatacaaac cccttgagaa
    23641 attccaacag tcttctgtag gctttcttca aaagcctaaa cacaaccctg agcgcaaacc
    23701 atagcaatac caaaaaagca gcggcggcta gaaacattaa tgccttttcc aatactacag
    23761 cgaacatcga aggctctgtc gcctcgatat ccgggatgat ctccatagga ccctgtcccg
    23821 gtaacccgcc catgccgggg cccggggtac caagcaggtt tggtaaaaac aaaataatag
    23881 ctaagaagcc ctgtcctacc tttcgggcca accacacgca gccatccttc aaatcattga
    23941 aatagcttat gaacattacc agggcaattg acagcagaat cagaatccta ttatttctaa
    24001 ggacagttga agaaaagatt ggaacagtcc cgtcaacggg ctgggatgct gtctttaatt
    24061 gctcggtgtt gattacaaac agcacaatta tggtgtatac aaacccggtc catgccaata
    24121 gtttaaaata tggttttaat gctggagtca aactatatat agaacccccc aatagataaa
    24181 gcgtcaagcc tatccataag gccatattgg ggaacatatc gccccatttg gtgcctcgaa
    24241 accgtacgcc ccgatcaaag atgaccatcc cggacaaaaa gaacaatatc atggcataga
    24301 gatttccgaa caataaaaat gccgacagtg aatttattaa tatccccaca aaaaaatgaa
    24361 tgtatctcgg ctttttcaag aggtaccccc cgagaataag ccctatgaga taaatggcag
    24421 ataaatcaac catccaaatc cagatttcct ttggaattaa caaaatccca acggtcaaca
    24481 atatcgggaa aaaggtgatg agctccaata acccctggag ccattttgtc attatgcttt
    24541 ttagcgtccc tattctaccc ttaatcattg cctacccctc cctcaggatc cctgagccgc
    24601 aatatttcta cggaattgcc catatcctct aattgtttta tctgactttt catacgctct
    24661 gttacgaagg aagttatgaa caaatagtca atagatcctg aaaatgtccc cgatgaccgc
    24721 tctttaatgt cctcctctaa aaaggtgtga aaggtgacgc ccctatctat tacgagtttg
    24781 gccatagtct caaacaagaa caataaatgt tcctccccat ttctcgggaa aacccttata
    24841 ggtagcccag gctcgtcaat ggtatatccg ttgctgccaa atcccgtatc tatcccctgg
    24901 gatatggcat gcctaacgat ggatgccgca taagagactc ccttctctat gcgttggggt
    24961 tcggtaatgg gcccccacat atcctcgcta atatccatat tcaagtagac cataatcctt
    25021 ggatctgcgg tgtaatcctt cttatgaacc tggagcctgc ctgtacgggc tgtagccttc
    25081 cagttgatat cgtttagggg atctcctgca gagtattccc gaaccccgga atacataaag
    25141 ggatcgtcca ctatccacct ctttaccact acatctccca tccagcttcg tgatagaaag
    25201 ggtatctcat caaggcgcat cagccttgga tataccataa gctcggcgtc caattcaaat
    25261 acccttgaaa catcgcgaaa gccgaacaga tccccacagg ttaatgcggc tgaattcaac
    25321 ctatagcatc ccctttttct gcatctaatt tgatgtcttc gattgatggc ggtatagggc
    25381 atgaggctga acaggctttt atgaaattgc tggtgcttaa tatctaagtt aaattgtctt
    25441 tgaaactcaa ggttttcact gatcttagat tcgatcctca gccagggtac aggaagcaac
    25501 tttctgttac tgatcctttc aaccatctca atttcctggc cctcaaaagc gctgttaaca
    25561 ttaaaatatc tattgtatcc tatccccctt agcataaacc tatcggttat ccatgcctgg
    25621 accgaaacca ctataagggt gacaaatatt atccattgta tgctcataat accgcctcgt
    25681 ccttagacgg gatcttttcg cttggtacgg gtacggcttt aaagatttgg tggattatat
    25741 cgtccaactg tccttccctc atccccaaga cattgcgcaa tattatcctg tgccctaata
    25801 cgggctttgc gatcttctta atatcgtccg gggacacata atcccttccc tgcaaaattg
    25861 catagacctg ggctgccttt agtagtgcct ggcttcccct tgggctgacc ccaagcatga
    25921 tatcaccgtg ttgtcttgtt acctcaacta tcctaacgat atatcgcaat atatcatcac
    25981 ttacaaatac ccgggcatag ctatgctggg cggcgatgat ttcatcactt tgggctacgg
    26041 gaccgatatc tataaaggga tccctttcct tgaatctttt cagtatctcc acaccctcat
    26101 cggtactggg gtaacccata tctactttta aaagaaacct atccaattgc gcctcgggta
    26161 ggggaaaggt cccctgggtc tctacggggt tttgagtcgc aagcaccatg aagggacgct
    26221 ctaaagatct ggtttcccca tccaccgtca cctgtttctc ttccatggat tctaaaaggc
    26281 tggattgggt tcttggtgtg gccctgttta tttcatcacc gaggagtatg ttggtaaaaa
    26341 tcggaccggg ccgaaattcg aattccccgg ttttttgatt ataaaagttt atcccgctta
    26401 aatcactggg aagtagatcc ggggtaaact gtaccctttt aaaggtacaa tctagggatt
    26461 tggaaagaga tttagcaaga agggtcttgc ctgttcccgg tacatcctcc aataatacat
    26521 gtcccgaaga gatgagggca atcaagagtt gatcaattac ttcgtcttta cccacgataa
    26581 cctttttgat gttatccttt atacgctgcg ccatgctcct aattttatca agttccattt
    26641 gattacctct ttctgcaaaa ttctcatatg actgtcactt gctatatatg ttctgaacct
    26701 cccatgacta aagtcacggg gttctagagt tcataagaac tcatttacgt tcatagcagc
    26761 taagccactt tgatacgtac aggcgtgtcc agttcgcctc taccgtatag ccctgttaag
    26821 ggcacaacgc tacttttatg ggattctcca attcatctca cgacttaagt cacgagtgtt
    26881 cttgaggagc ctaataaatc tcgggcatta tccgagccct gatcgaactt gttttataga
    26941 tagctattgt taaatatgtt ctttgccttg gaacattccc aaggttccgg atattacgcc
    27001 ggtcattctg tcccatctaa ggacaaagga tatgtatccc attccattat acactcaccg
    27061 ctattctttc tcaatgatat ttactcattg ttgtaaatga ataaggtgtg cacagcggaa
    27121 ccgtccccct gtgtgcatcc ccctgtgttt cttcttatga tttatggg
//
LOCUS       DASRNX010000075         4272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_173959,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000075 DASRNX010000000
VERSION     DASRNX010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173959"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            390..1244
                     /locus_tag="VFD89_03090"
     CDS             390..1244
                     /locus_tag="VFD89_03090"
                     /inference="COORDINATES: protein motif:HMM:NF025469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4277 domain-containing protein"
                     /protein_id="HZJ57206.1"
                     /translation="MVDPNIVETYTIGKEGFIVPLCESLGVPEIINKTLGSVNGRPPD
                     IPYGVDAMMMMVNMCDDHMPLSRLHEYYQYRDLEGLFHHPIKLEQINDDRFGGFLDLL
                     HGAGCKKIFSQIAGKAITLYGIQVTNINFDTTSKVMWGLYETYEGTEGVIKMDFGHSK
                     DRREDKKQIKFGIGCANGLVVDATVLSGNKYDKTYNTDTISALDDTLHNLSVDKGNFY
                     YIADSALFSKENLDLSTEKGIRLITRIPDNVLIAKESMEEAVDILDTDEKNAAGVVAL
                     QKVKITKY"
     gene            1364..1765
                     /locus_tag="VFD89_03095"
     CDS             1364..1765
                     /locus_tag="VFD89_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57207.1"
                     /translation="MLREYKTQDSVEKKFQQLKSPQFVNSIFLESPDRIEAFSYLMLI
                     CILMLSLAEYVVRRGLMQDDDYIIGPRKTKMNRPTQRVIYDVFYAVMIRVICYPDRPW
                     ERSYARPLNESIKKILRYLKIPDDTFIKGGS"
     gene            complement(1966..4053)
                     /locus_tag="VFD89_03100"
     CDS             complement(1966..4053)
                     /locus_tag="VFD89_03100"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HZJ57208.1"
                     /translation="MRTNTPNEYVFGQGQSSLTVFAEREGTPYLRNLVGMECGNYYRG
                     AVDEDLLDEIFYLNTNRGYFTPRNSNLIRSEFSEDEIFFEWSIPCGNLSLTSTWTFHK
                     ASGVWRRQDSLHNAGNEGIYILSSGSRFIFSPGAYELYSQGSRWCGENQGVWQDINHA
                     TLVLSSKGGRTNQGNSPFVMLRDNENGRSISFHIIPRGDWLIRISSHVSTNDRPPYII
                     AELGHSDANLNLRLKPGQNIRLPDILFYGTDHSQDWYDAGKFHSYLLENSPQNRKGIT
                     PIIYNTWFDQFEFLDVERLRMQLAGAKEIGCEVFAVDAGWYGAGEAPWAQQVGDWREK
                     RAGAFYGEMRGFAEEVREAGLGFGIWMEPERISKEAPIFKESPKLFARGEDGFYYPKL
                     WEDEAYAYTKDEIRRLIEDYDLAWIKIDYNFSFGVDDTGSNFLLYYSAWNDLFDELRQ
                     EYPRVFFEGCASGGMRFELNSMERFDCHFLSDSVDPIDVLRISQGALLKYLPGNIGKW
                     AVLRGYSNTIPKYGLPIEKAPHSIVAPSGSGWDNCYVANVDFILRVAMCGVLGVSGDL
                     TTLPEEILERIKWNISIYKKWRDLIRNSIAYMLEPPRLQHDRGGWVAIQLMDRSGDEG
                     LLFVYRLNDGCDTKTYRLKALNGDDLYIIYDADNPEAILESSKGAALMGVGTKFSVDT
                     KNSAKVYIIKKTR"
BASE COUNT         1208 a          988 c          818 g         1258 t
ORIGIN      
        1 gtaatgcaat ctttaatttg tatcacgaac attcgctcat cttcgcttac atctccaaca
       61 cgctttttgc taaggttcct aatctcattc atacaaatat ccctcccttc gattaaattc
      121 gtgctacctt ggcatataaa agagctgcta ttgccttgaa caataacagc ttactaatta
      181 ataggacgag cttggaggag cgggaatagc tatatcatag agtgagagta gctaattttt
      241 aatgaatatt cagctttcta tttaaatccc atactccgct gaactaaaat tggtaaatct
      301 gtattttaca tgcaattaat tgccaaaagg gttttcaaga tttatgtcga atacctcctt
      361 aaagtgaaaa gttttgggag gaaaacgcta tggtggatcc aaacattgtt gagacctata
      421 cgatagggaa agagggcttt atcgtgccct tatgtgaatc tttaggagta cctgaaataa
      481 tcaataaaac acttggctca gttaatggcc gccccccgga tatcccttat ggtgtcgatg
      541 cgatgatgat gatggttaat atgtgcgatg atcatatgcc tttatcaagg ctacacgaat
      601 attaccagta cagagatcta gagggtctgt tccatcatcc gataaaacta gagcaaatta
      661 acgatgaccg ctttggtggt ttccttgacc ttctccacgg agctggctgt aagaagatat
      721 tttcacaaat tgcgggcaaa gccattacac tgtatggcat acaggttaca aacatcaatt
      781 ttgacaccac ttccaaggtc atgtggggcc tgtatgaaac ctatgaaggt acagaagggg
      841 tcataaaaat ggactttggc catagcaaag atagaaggga agataaaaaa caaattaaat
      901 ttggtattgg ctgtgccaat ggtctggtag tagatgcaac tgttctttct gggaacaaat
      961 atgacaaaac ctacaacaca gataccatta gcgccttaga tgataccctt cataatttaa
     1021 gtgtagacaa aggtaacttt tactacattg cagacagcgc cctattttcc aaggagaatt
     1081 tggatctatc cacggaaaaa ggtataaggc tcataacccg tatccccgat aatgttctta
     1141 tcgccaaaga aagtatggaa gaagcagtag atatccttga taccgacgag aagaacgccg
     1201 caggcgtggt cgccctgcaa aaggtgaaga taacaaaata ctaggatacc gctattttgt
     1261 agagcttagc cctgttctaa atcaagaaaa aacaacgaaa gccattaata aggcttgctg
     1321 tttcgtacta tgttccaatg atcttgggat gactggcgag gcattgctta gggaatacaa
     1381 aacccaagat agcgtagaaa agaagtttca gcaattgaaa tcaccgcaat ttgtcaattc
     1441 aatcttcctt gaatcgcctg atcgtataga ggcattttct tacctgatgc tcatctgcat
     1501 acttatgctg tcactggcgg agtacgttgt aaggcgcgga ttaatgcagg acgatgacta
     1561 cattattggg cccagaaaga ccaagatgaa tcgtcctaca cagcgtgtca tatatgatgt
     1621 tttctacgct gttatgattc gggttatatg ttacccggat agaccttggg aaaggagtta
     1681 tgcaaggccc cttaatgaat caattaaaaa gattctaagg tacctaaaaa tccccgatga
     1741 cacctttatc aaggggggta gttagcgttt ttaaagacga tattttaaat cttaaatttc
     1801 caaggtacat tcagcggagt atggggtaca tgctgagatg tgcgaggact ggggatgtcg
     1861 gttagacaat acaaattaca acccacacga aagaaagcca ccgggatact tttcttcggt
     1921 ggcttttcta taactgccaa cagtttttta tatagttcgg ccagcctatc gggtcttttt
     1981 aatgatatag actttcgcac tattctttgt atcaacgcta aatttcgtac cgactcccat
     2041 caatgcggcc ccttttgatg actccagtat agcttcgggg ttatcggcat catatatgat
     2101 atagaggtca tctccattaa gtgcctttag cctgtatgtt ttagtgtcgc acccatcatt
     2161 caatctatag acgaataaca gcccctcatc cccgctcctg tccataagct gaatagccac
     2221 ccaacctcct ctatcatgct gcaaccttgg aggctctagc atataggcta tagaatttct
     2281 tattaagtcc ctccattttt tatatatgga tatattccat tttattctct ccagtatttc
     2341 ttcgggcaaa gtggtcagat ccccgcttac ccctaaaact ccgcacattg ccacccttaa
     2401 tataaaatcc acattagcga cataacaatt gtcccacccg gaacctgagg gagcaacaat
     2461 ggaatgaggt gccttttcaa taggcaatcc atatttgggg atggtattac tatagcctct
     2521 gagtaccgcc catttgccaa tattccccgg cagatattta agcaaagccc cttgggatat
     2581 tcgcaagaca tcgatgggat ctacgctatc gcttagaaaa tggcaatcaa atctttccat
     2641 gctgtttaac tcgaatctca tacctccgct ggcacaacct tcgaagaaca cccggggata
     2701 ctcttgtctt aactcatcaa atagatcatt ccatgcgcta tagtacagga gaaaattgga
     2761 acctgtatca tcaacaccga aactaaaatt atagtctatt tttatccacg ctagatcata
     2821 atcctctatg agcctgcgga tctcgtcctt tgtatatgcg taggcttcgt cttcccatag
     2881 ctttggataa taaaaaccat cctccccccg ggcaaatagc ttggggcttt ccttgaatat
     2941 aggggcctcc ttgcttatcc tttccggctc catccaaatt ccaaaaccca ggcctgcctc
     3001 ccttacttcc tcagcgaacc ctctcatttc cccataaaaa gctcctgctc gtttttctcg
     3061 ccaatcacca acctgctgcg cccagggggc ttcacctgct ccataccatc cagcatccac
     3121 agcaaaaact tcgcatccta tttctttagc tccggctaac tgcattctga gcctttccac
     3181 atcaaggaat tcaaattgat caaaccatgt attgtatatt atgggcgtaa tacccttcct
     3241 attttgaggg ctgttttcca aaaggtagct atgaaatttg ccggcatcat accagtcttg
     3301 gctatgatcc gtcccataga ataatatatc cggaagcctg atattttggc ccggctttag
     3361 tcttagatta agattggcat cgctatgccc cagctcggcg attatatatg gggggcgatc
     3421 attggtggat acatgggacg aaattcttat taaccaatcc cctctaggaa ttatatgaaa
     3481 tgatatggac ctaccgtttt cgttatctct cagcattaca aaggggctat tgccctggtt
     3541 tgtcctcccc cctttactgg atagaaccag ggtggcatgg ttaatatcct gccatactcc
     3601 ctgattttca ccgcaccacc tgctgccttg gctatatagt tcgtacgcac ctggtgagaa
     3661 tataaacctt gatccagagc ttaagatata tataccttca ttccctgcgt tatgtaagga
     3721 atcctgtcta cgccagacac cgctagcctt atgaaaagtc caggtgcttg ttagggataa
     3781 attaccgcag ggtatactcc attcaaagaa gatttcatct tcgctaaatt ctgatcgtat
     3841 taggtttgaa tttcttgggg taaaataccc cctattcgtg tttagataga aaatctcatc
     3901 taacagatcc tcgtccacgg ctcctctgta gtagttccca cactccatgc ccactaagtt
     3961 tctgagatat ggggttcctt ccctttcagc aaataccgta aggctgctct gaccttgtcc
     4021 aaaaacgtat tcattcggtg tatttgttct cattgcaatc tcccctaatc caaataatcc
     4081 gctacaataa gttgatatcc tttgctcttt ttataatact ccaatcacta aatccttgta
     4141 gtgacaatgg ctcccttcga ggatggacat acccacctgg cctgtcaaat aaggagattt
     4201 tgtatctgta tagacaatca attcttgatc tccttcggta atattaattt cgggaccctt
     4261 tactcggacc tt
//
LOCUS       DASRNX010000076         4707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_174018,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000076 DASRNX010000000
VERSION     DASRNX010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174018"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..721)
                     /gene="bfr"
                     /locus_tag="VFD89_03105"
     CDS             complement(254..721)
                     /gene="bfr"
                     /locus_tag="VFD89_03105"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HZJ57209.1"
                     /translation="MKSDERLIETLNSLLADELAAINQYIVHAEMCEDWGYGKLHEKL
                     EKRAIDEMKHAEKLIGRILFLGGNPVVTSLSNINIGKEVPAQVENDRVSELAAIKAYN
                     RAITLAGELEDHATRDILIQILNDEDRHMDEIEELQDQIEHMSLPIFLTTQVG"
     gene            943..1302
                     /locus_tag="VFD89_03110"
     CDS             943..1302
                     /locus_tag="VFD89_03110"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZJ57210.1"
                     /translation="MFYVIFEVVVNQEHMDEYLSLAAGLRESLMKNKGFIRAERFSSI
                     VDNRKLLSLSVWESEEAISDWRNRAEHRLGQRQGRDSFFESYTITVTSPVRTYTDTDR
                     EEAPKDSNEAFGSETYK"
     gene            1499..2428
                     /locus_tag="VFD89_03115"
     CDS             1499..2428
                     /locus_tag="VFD89_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HZJ57211.1"
                     /translation="MKIEKITIVGMGALGVMYGDFLTEKLGKEHVGFVANRERINKYK
                     KEGVFCNGRRCDFTVVDEETQGEPTDLLIFAVKATGLEEAIHTVRNQISENTIIISVL
                     NGISSEDVIGREYGMDKIIHCTVQGMDTTKIGNKVKYMQFGNICLGLDEENEDKKDKL
                     EAVLDLFRKIEMPYVLEEDIMRRIWGKFMLNVGLNQSVMIYEGNYGTVQKPGRARDLM
                     QDAMQEVITLANMENININQKDFDDYIELVDNMNPSGMPSMRQDGLQKRKSEVELFSG
                     TVIRLADKHNIDVPVNREIYETVKKVEDGYNAI"
     gene            complement(2538..3710)
                     /locus_tag="VFD89_03120"
     CDS             complement(2538..3710)
                     /locus_tag="VFD89_03120"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HZJ57212.1"
                     /translation="MGFNFDEVIDRRNTNSIKYDFMRERGKAEDLIPLWVADMDFKVP
                     DEVTMALARAVEHGIFGYSDSRADYFNAVHRWHKDRFNYDIKEEWLVKSPGIVFALAT
                     AIKAFTEKGDGVIIQQPVYYPFSGMILNNERRVVNNPLIYKDGKYYMDFDDFEEKVIR
                     ENVKLFILCSPHNPIGRVWTEEELVRLGDICLRHNVIVVSDEIHADFIYGNNKHSIFS
                     GIRPEYLNNTVLCTSPSKTFNMPGLQVSNIFIANKKLRLKFSNEIRKTGYSHLNTLGL
                     VACTAAYEHGAPWLEALKDYLWENVRFVGDFLSSQIPRVKLVEPEGTYLLWLDFSALG
                     LGDDELEELIEKKAGLWLNPGRAFGTGGQGFQRLNIACPRQVLDRALAQLKQAIDS"
     gene            4045..4242
                     /locus_tag="VFD89_03125"
     CDS             4045..4242
                     /locus_tag="VFD89_03125"
                     /inference="COORDINATES: protein motif:HMM:NF020940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HZJ57213.1"
                     /translation="MLFATVRELQETVFSVMIIRLFGTAEELNAVQAFIADAGVSVER
                     ISLSELQEVNTYDTKISGHYA"
     gene            4211..>4707
                     /locus_tag="VFD89_03130"
     CDS             4211..>4707
                     /locus_tag="VFD89_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZJ57214.1"
                     /translation="MILKYLGITPEQMALAAGQTLYMLFWGLLLGSLLGIPLGILLAL
                     TRNNGVLENRFVYGSLNYLVNALRSIPFIILLVAIMPFTKAIIGTRIGTKAALVPLIF
                     YIVPFLTRLVEGSLLEVGEGIIETAVSMGATPLQIIVHFLIPEALGSLVLSLTTGAIG
                     LLGATA"
BASE COUNT         1357 a          972 c         1064 g         1314 t
ORIGIN      
        1 aaggtccgag taaagggtcc cgaaattaat attaccgaag gagatcaaga attgattgtc
       61 tatacagata caaaatctcc ttatttgaca ggccaggtgg gtatgtccat cctcgaaggg
      121 agccattgtc actacaagga tctagtgatt ggaagataat aaaaagacta gaagaattgt
      181 tgatagctat agaaaagcca ccgaaggaaa tatcccggtg gctttctttc gtgtgggttg
      241 taatttgtat tatctaaccg acttgggtag ttaggaaaat cggaagggac atatgctcaa
      301 tttggtcctg cagctcttca atttcgtcca tatggcgatc ctcatcgtta agtatttgta
      361 tcagtatatc ccttgtggca tgatcctcta gttcacctgc taatgtgatt gccctattat
      421 atgccttgat agctgccagt tctgagacac gatcattctc tacctgggcc gggacttcct
      481 tgccaatatt gatattgctc aagctggtta caacggggtt gcctcccaga aacaaaatcc
      541 taccaataag tttctctgcg tgcttcattt cgtcaattgc ccgtttttcg agtttttcgt
      601 ggagttttcc atatccccag tcctcacaca tctcagcatg tacaatatat tgattgatgg
      661 ctgccagctc atcggctaac aaagaattta gcgtttctat taacctctca tcgcttttca
      721 tgaatagtat ccccctttac tttgtatata taattggttt actatattat accattgtat
      781 tgcatcccaa tcaacaaacc aatcagaaaa atgataattt agtcggttgt ccatgggtaa
      841 ttgtgaaaca aaactacttt ttttgcaaat ataaagtaca taattcggtt attattgata
      901 taattgttct gatagtatat cggtgaaagg gagattaaaa gtatgtttta tgttattttt
      961 gaagtggtag ttaaccagga gcatatggac gagtacctga gcctcgcagc cgggcttagg
     1021 gagagcctta tgaagaataa gggctttata agggcagaac gattttcaag catcgtggac
     1081 aatagaaagc tacttagcct gtccgtatgg gaaagtgaag aggccataag tgactggaga
     1141 aaccgggcgg aacatcgctt gggccaaagg cagggaaggg attcattctt cgaaagctat
     1201 acaataaccg ttacatcacc ggttcgcact tataccgata cggataggga agaggcgccc
     1261 aaggattcca acgaagcttt tggttcagag acctacaaat gatcttacca aacgctcgta
     1321 aaagactttg attggtaaaa taatattagg gtaacaatta tcgtagctga aggacctaaa
     1381 tccaatgatt caaaaaataa attcagagta tagagcctaa agaccagtcg aaaatatgaa
     1441 aagcaatgca ctaatttagg tatcagataa ggcaccagaa aaaggaggac taccatctat
     1501 gaaaattgaa aagataacaa tcgtaggaat gggagcatta ggggtaatgt atggggattt
     1561 tttgaccgag aaacttggga aagaacatgt ggggtttgta gccaataggg aaaggattaa
     1621 caaatataaa aaagaaggcg tattctgtaa cgggcgaagg tgtgatttta cggtagttga
     1681 cgaagagacg cagggggaac ccacggattt acttatcttt gcggtgaaag ccacggggct
     1741 ggaggaggca attcataccg tacgaaatca aatatcggaa aacaccatta tcatatctgt
     1801 tctaaacggc atctccagcg aggatgtaat aggcagggaa tatggaatgg ataagataat
     1861 ccattgcacc gttcaaggta tggatacgac aaagatcgga aataaggtta agtatatgca
     1921 attcgggaat atctgtctgg ggctagatga agaaaatgaa gataagaagg acaagttaga
     1981 agcagtattg gatttattcc gtaagatcga aatgccttat gttctggaag aggatataat
     2041 gcgcaggatt tggggtaagt tcatgctcaa cgtcggattg aatcaatcgg tcatgattta
     2101 tgaggggaat tatgggacag ttcaaaaacc aggcagagcg cgggatctga tgcaagatgc
     2161 catgcaggaa gtaataaccc tggctaatat ggagaatatc aacataaacc aaaaggattt
     2221 tgatgactat attgagctgg tagataatat gaatcccagt gggatgccgt ccatgagaca
     2281 ggatggcttg caaaaaagaa aatcagaagt cgagttattt tcaggtaccg tcattagatt
     2341 ggcagacaag cacaatatag atgtgccggt taacagggaa atctatgaga ccgtcaaaaa
     2401 agtagaagat gggtataatg ctatatagaa acccagggaa gcccccatcc attcaggaat
     2461 gggggtttcc tataaaccga tattaggggt acattaaaac gtcggaagca agcgtacaca
     2521 ttttaatgaa gcgggcgtta tgaatctatc gcctgcttta attgcgctaa ggcccggtcc
     2581 aatacctggc gaggacacgc aatattaagc ctttgaaacc cctggccccc tgtcccgaag
     2641 gccctgccgg ggttcagcca cagccctgcc tttttttcta tcagttcttc caattcatca
     2701 tcacctaacc ctagggccga aaaatccagc cacaagaggt aggtaccctc gggttccacc
     2761 agtttaaccc ggggtatctg gctgctgaga aaatccccta caaaccttac gttttcccat
     2821 aggtagtctt ttaacgcctc cagccacggg gcaccatgtt cgtaggcagc tgtgcaggcc
     2881 acaaggccca gggtgtttaa atggctatac ccagtttttc ttatttcatt tgagaactta
     2941 agcctcagtt ttttatttgc tatgaaaata ttggatacct gtagccccgg catattgaag
     3001 gtcttgctgg gtgaggtgca tagcacggta ttattaagat attctggcct gatgccggaa
     3061 aagatgctgt gcttattatt tccgtatata aaatctgcgt ggatttcatc agagacaact
     3121 attacattat gccttaagca gatatcccca aggcgtacca gctcctcctc ggtccatacc
     3181 ctgcctattg gattatgagg gctgcaaagt ataaatagct tgacattttc tcttatgacc
     3241 ttctcttcaa aatcgtcaaa gtccatataa tatttgccat ccttgtatat aaggggatta
     3301 ttaacgaccc ttctttcatt atttaggatc attcctgaaa aggggtaata cactggctgc
     3361 tggatgatga ccccgtcccc tttttccgta aaagctttta tagcggtagc cagggcaaag
     3421 actattcccg gtgatttaac cagccattcc tctttaatat cgtaattaaa ccggtcctta
     3481 tgccacctat gtactgcatt aaaataatcc gctctgctgt cggagtagcc aaagatgccg
     3541 tgttccacgg cccttgccaa tgccatggtg acttcatcag ggaccttaaa atccatatca
     3601 gcgacccaca aggggatcag atcctctgcc tttcccctct ctctcataaa gtcatatttt
     3661 atagagttgg tatttcttct gtcgatgacc tcgtcaaaat taaagcccac tataatccct
     3721 ccgtttccag aatgtcggtt agccgaatct catatcatgg gacaggccct gctatgacac
     3781 cggataggac tccctgtcat tcgattatta atatgtatac taaagcaacc ccaaatgatt
     3841 gtcaatgtga tttatggcga ttgagatgta cccatggctc caatatgact atagggcagc
     3901 gccgcaattg ccagtgcgat tgcttccaat gttataactg aggctcgtgt tataaccggg
     3961 gcccggaaaa tacatctatg atttaatatt tcacggttat tctttctatt aattatttta
     4021 gtccacaccc acccagaaaa cagcatttta tttgctacgg ttcgggaatt gcaggaaacg
     4081 gtgtttagcg taatgattat aagattattt ggcacagcag aggagctcaa tgccgtacag
     4141 gcctttattg ccgatgcggg ggtttccgtt gaaaggatta gtctgtcaga gctacaggag
     4201 gtgaatacat atgatactaa aatatctggg cattacgcct gagcaaatgg ccctggccgc
     4261 agggcaaacc ctttacatgc tattttgggg actgctcttg ggatctttac tgggaatacc
     4321 ccttggcatc ctgttagctt tgacccgaaa taacggggtc ctggaaaacc gatttgtgta
     4381 cggcagcttg aattatttgg tcaacgcctt gcgttccatc ccctttatta tcctgctggt
     4441 agctataatg ccctttacaa aggcgatcat aggcacccgt atagggacca aggcagccct
     4501 ggtgcccctg atcttttata tcgtgccttt tttaacccgt ttggtggagg gatcactact
     4561 ggaagtaggg gagggaatta ttgagactgc ggtatccatg ggggcgactc cgctgcagat
     4621 aatcgttcat tttttaatcc ccgaggcctt gggctctcta gtactatccc ttactacagg
     4681 cgccataggc ctcttgggtg caacggc
//
LOCUS       DASRNX010000077         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_333635,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000077 DASRNX010000000
VERSION     DASRNX010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333635"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            397..1437
                     /gene="argC"
                     /locus_tag="VFD89_03135"
     CDS             397..1437
                     /gene="argC"
                     /locus_tag="VFD89_03135"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZJ57215.1"
                     /translation="MINVSVLGVTGYAGIELIRLLSSHPEVKICNMTSHSYAGKTLGE
                     VYPHFMNSYDIPLTKVEIEKITKDVDLVFTCLPHGASSEIIPDLYKKGVKIIDLSGDF
                     RYISPNIFEKWYGYEHPNPLLLNKSAYGLPELHKEEIRGNRLIGNPGCYPTGSILPLA
                     PLIHHDLIHNNSIVIDAKSGATGAGRASSVELNFSETHENVKAYKVAAHRHTSEIEQE
                     LGLLAGGDVTLSFTPHLLPIKRGILSTIYAGLKGSLSFHDVYEIYRDFYDDAPFVYLY
                     PEGKLPEVKFVNGSNGCHIGFALDARTNRIILVSAIDNLIKGAGGQAIQNMNIMFDLD
                     EDLGLPKIGWYL"
     gene            1553..2425
                     /gene="argB"
                     /locus_tag="VFD89_03140"
     CDS             1553..2425
                     /gene="argB"
                     /locus_tag="VFD89_03140"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZJ57216.1"
                     /translation="MNNLIHKANILVEALPYIQKLSGKTVVIKYGGNAMVHEEIIKTI
                     MQDITLLKYVGVNPVVVHGGGPKINETLDIMNIKSEFHKGLRVTTEDAIDVVEMVLAG
                     SINKDIVSQLNSLGGKAIGLCGKDGNLIEVEKKVSIDSVDLGYVGEIKKINSKIVEIL
                     ARDEYIPVIAPIGTDNRGQSYNINADTAAGEIAAALKAEKLIFLTDIDGIYLDPKDDE
                     SLISVISVPEIYELMDKGIITGGMIPKVLGCIKGIEQGVNRTHILNGTIPHPILLEIF
                     THKGIGTMVTKGGF"
BASE COUNT          735 a          562 c          570 g          668 t
ORIGIN      
        1 gccttttttc gacagtctat ggccttcatt ctatatgatc acctgtgtag tggcatgttt
       61 ttagattatg atatcccccc tttattttcc ttcccttatt tccccaatga ttcatatcag
      121 ttcccccaaa ccctattttg attgttttgt tttcccgctg ccccaaaaaa ttgtttcata
      181 ttctcgctgt cccctaaaaa tagaggatgg ataaggcgaa tcaattaata cggaaaccca
      241 aaattcataa ctacaaattg gcccttgcat ttatattcgc cctgttgtat aattattagc
      301 aattggctat atttatactc ctattctagg ggatagaaag cctttttcgc aacgtgtata
      361 gtatttatcc gcatttttaa agggggcttt tacaaaatga tcaatgtaag cgtactgggc
      421 gtcaccggat atgcagggat agagcttata aggctcctct cctctcaccc agaagttaaa
      481 atatgcaata tgacctcgca tagttatgca ggtaagacct tgggggaggt gtatccccat
      541 tttatgaact cctatgatat tcctcttaca aaggttgaga tagagaaaat aacgaaggac
      601 gtggatttag tcttcacatg tcttccccac ggagcatctt ccgagataat tccggattta
      661 tataaaaaag gggttaagat cattgacctc agcggggatt ttcgctacat ctccccaaat
      721 atctttgaaa aatggtatgg atatgagcac cctaatcccc tgcttttaaa taaatccgct
      781 tatggccttc ccgagctcca caaggaggag ataaggggca ataggctcat aggcaatccc
      841 ggttgctatc ctaccggttc catactgccc ctagccccgt tgatacacca tgacctcatc
      901 cataataact ccatagttat cgatgccaag tcgggtgcca ccggtgcggg gcgggcaagt
      961 tccgtggaac ttaatttttc tgaaacccat gaaaacgtca aagcctacaa ggtggccgcc
     1021 catagacata cctccgaaat agagcaggag ttggggcttc tggcaggggg agatgtaacc
     1081 ctttccttta ccccccacct tttgcccata aagcgtggga tattgagcac aatctatgct
     1141 ggccttaagg gttctctgtc ctttcatgat gtatacgaga tatacaggga tttctacgat
     1201 gatgcgccct ttgtatacct ataccccgag ggtaagctgc cggaagtaaa gtttgtaaac
     1261 ggctctaacg ggtgtcatat tggctttgct ttagatgcaa ggaccaatag gatcatcctg
     1321 gtttcggcca tcgacaacct tataaagggt gccggcggcc aggcaataca gaatatgaat
     1381 attatgtttg acctggatga ggatttaggc ctccccaaaa tcggctggta cctgtaaaag
     1441 ataccccttg caaggtttta atccgattaa aaccggacaa ctaagcccat gaaaagcccc
     1501 gcttccggag aggtattgtg aaaccgaatc aatttttgaa atgaggactg ttatgaataa
     1561 tcttatacac aaagcaaaca tacttgttga ggcccttccc tatatacaaa agctcagcgg
     1621 taagaccgtg gtcataaagt atggcggaaa tgccatggtc cacgaggaaa taataaagac
     1681 catcatgcag gatataaccc ttttaaaata cgtaggcgtc aatcccgtag tggttcacgg
     1741 cggcggtcca aagataaatg agaccctgga tataatgaac ataaaatcgg aatttcataa
     1801 gggccttagg gtcactaccg aggatgccat agacgtggtg gagatggtct tggcaggttc
     1861 tataaacaag gatatagtct cccagctcaa cagcctaggc ggtaaggcaa tcgggctctg
     1921 cggtaaggac gggaacctca tcgaagtgga aaaaaaggtt tcaatagaca gcgtggactt
     1981 ggggtatgta ggtgagatta aaaaaatcaa ctccaagata gttgagatac tggcccggga
     2041 cgaatatatc cccgtcatcg cccccatcgg gaccgataac aggggccaaa gctataacat
     2101 aaatgcggat acggccgccg gggagatagc cgccgcccta aaggcggaaa agcttatctt
     2161 ccttacggat attgacggta tatacttaga cccaaaagat gatgaatcct taatctcggt
     2221 tatctctgta ccggaaatat atgaacttat ggacaaggga atcatcacgg gaggcatgat
     2281 acccaaggtg ttaggctgta taaagggtat cgagcagggc gttaatagga cccatatact
     2341 aaacggcacc atcccccatc ccatactgct ggagatattc acccacaagg ggataggaac
     2401 tatggttaca aaaggaggct tttaaggtgg attatatgga ttataaggac gtacaagagc
     2461 tggatgaaaa atattatttc aatacctacg ggatgagact gcccgtatcc tttgaatatg
     2521 gcgaaggctg taccc
//
LOCUS       DASRNX010000078         3695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_386630,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000078 DASRNX010000000
VERSION     DASRNX010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386630"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1581
                     /locus_tag="VFD89_03145"
     CDS             280..1581
                     /locus_tag="VFD89_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZJ57217.1"
                     /translation="MRNVAFERNLEVRHEVDVLVVGGGPSGVAAAVSASRQGAKVFLA
                     EATSCLGGMGTSGLVPAFMQFTDGENFLAGGIGEEILNKMWEYGGKIEGSEYSIKVEA
                     LKRVYDDIVTEAGVNFALHTHMVAVDADDGRVNFVVFAAKSGMFVVKAKVYIDGTGDG
                     DLCAWAGAEYEKGDENGEMMAGTLCSIWSNINWDKVKRPDNRRLNEAIEDNIFTYEDR
                     HLSGMWRTGKTLGGGNIGHTYDVDATDERSLTKSLIWGRKSLTEFERYYREYLDGFEG
                     IELAATGALLGIRESRRIIGDYVMVLDDFMNKSDFHDEIGRYSYPVDIHPSDTSEEGF
                     KKFEKEFYNLRYKDGESYGIPYRILTPKSLENVLVAGRCVSCDRYIQSSIRVMPGCYI
                     TGQAAGVAAAIAAEQETDTRDIDVRLLQSRLKELGAFLPNF"
     gene            1735..2082
                     /locus_tag="VFD89_03150"
     CDS             1735..2082
                     /locus_tag="VFD89_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HZJ57218.1"
                     /translation="MKRYMVLFLCALLAFGIIAGCGDSKGKEQFSFIATVIEKGESYI
                     LVEPDEGSSELNSADKISVSIGDATIIDDEGKDITIQDIDEGDKVKIHYDGQIAESYP
                     AQIHRCHRIVLVK"
     gene            2107..2760
                     /locus_tag="VFD89_03155"
     CDS             2107..2760
                     /locus_tag="VFD89_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HZJ57219.1"
                     /translation="MCGRFLLDTEIREIIRTYKIQKNRNTDYKSGEIFPSTNAPIVFE
                     DGERTIASAKWGFPYGFKKGIVINARSETIMQRPMFKNSFYTARCVIPANLYYEWKDE
                     GGKRKVRHGIGVHDMDLISLGGIYKVSLDENGLMEQMTFVIITAKADTAIRSIHNRMP
                     LIIGDRDIEPWLDKGADIKYIEDILKPKMDYSFSIRRFEDKSAEDRDNEDHQQLKMF"
     gene            complement(2852..3541)
                     /locus_tag="VFD89_03160"
     CDS             complement(2852..3541)
                     /locus_tag="VFD89_03160"
                     /inference="COORDINATES: protein motif:HMM:NF021120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhcN/YlaJ family sporulation lipoprotein"
                     /protein_id="HZJ57220.1"
                     /translation="MKNRIRVLLSVLLLISTIALFGCTTNRRPITPAPGQTRTVPGPT
                     RVTPPTTPAPGIQRPVPAPTRVTPRTTNPNDPGTVTPGTNGIMTPGGPGTMTPGTPRT
                     TTPGPTTPGTVRRPSTTNTAGDRAKAIARDVAKEKNIQSASCVVTGDTALVGLQFEKQ
                     YKGKVTDAIKKSVDRRVKKLESTIKKVVVTADPDLLSRIKTMAKDIEGGKPLSGFTNE
                     IEEIVRRINPF"
BASE COUNT         1063 a          664 c          935 g         1033 t
ORIGIN      
        1 taattattac atgatgtatg tcaagaggat ttacaatcaa agtaaatccc ttcagtttta
       61 ttgaattatg ctggaaagta tagtaaaatt ggatatacaa taaagacaag tcacttatct
      121 tcacttaggt acgacatcta attaaaagac ccggagttat ccaaacaaaa gttcataaaa
      181 atgttggacc ataaagacat tgctattcaa gtaacaaaac aactacataa aacgatccta
      241 tgacaaattc atactaaaat accatggggg tgcttaaata tgagaaatgt tgctttcgaa
      301 aggaatctag aagtacggca cgaggtagac gttttggttg ttggaggcgg cccatcgggg
      361 gtggctgctg cagtctctgc gtccagacag ggagctaaag tttttttagc agaggctaca
      421 tcctgcctag ggggtatggg caccagcgga ttagtccctg cctttatgca gtttactgat
      481 ggtgaaaact ttttagcggg cggtataggt gaggaaatcc taaataaaat gtgggagtac
      541 ggcgggaaga tagaagggtc ggaatattcc attaaggtag aggcattaaa gagggtatac
      601 gatgatattg taactgaagc gggtgttaat tttgccctgc atacacatat ggtagccgtt
      661 gatgctgatg atggacgtgt taactttgtt gtttttgcag ctaaaagcgg aatgtttgtt
      721 gtaaaagcaa aggtatatat tgacggtacc ggagacggcg atctatgcgc ttgggccggg
      781 gcagaatatg aaaagggtga tgagaacggg gagatgatgg caggtaccct ttgtagcatt
      841 tggtctaata tcaactggga taaagtcaaa aggcccgata atagaagact aaatgaagcg
      901 atagaagaca acatatttac gtatgaggat cgtcacttgt ccggtatgtg gcgtacgggt
      961 aagactctag gcggcggcaa tattggtcat acatatgatg tggatgctac ggacgaacgc
     1021 tctctaacga aatctttgat atggggtaga aaatcattga cggaatttga aagatactac
     1081 agggaatatt tagacggttt cgaaggcata gaattggcag ccactggggc cttattaggt
     1141 atccgggaga gcaggcgtat tataggggat tatgtcatgg tcctagatga ttttatgaat
     1201 aaatccgatt tccatgatga gataggtaga tattcctatc cggtagatat ccatccttca
     1261 gatacctcag aagaaggttt taaaaaattt gagaaggaat tctataacct gaggtataag
     1321 gacggggaaa gctatggaat tccctacagg attttaacac ctaagagcct agaaaatgta
     1381 ctggtagcag gacgatgcgt aagctgtgat cgatatatcc aatcctctat acgagttatg
     1441 ccgggatgct atattacagg ccaggcggca ggtgttgcgg cggctatagc agcagagcag
     1501 gaaacggata ctagagacat tgatgtaagg ttattacaat cccgccttaa ggaattaggc
     1561 gcattcttgc caaactttta atccaattta ctgagccgtg gatatcttta actaataggt
     1621 agcggtatgt ttaagtatgg gacagctaat aattgcctat gtttcgctaa tcgaattatc
     1681 gaatgcaaca aatggcctta tcctccgtct gatagggtag ggaggattta tactatgaaa
     1741 agatatatgg ttttatttct atgcgccctg ttagcctttg gcatcatagc agggtgcggt
     1801 gattctaagg gaaaggaaca gttctcattt atcgctacag ttatagaaaa gggcgaatcc
     1861 tatatattgg ttgaacctga cgaaggttct tctgaattaa actccgcaga taagatatca
     1921 gtatccatcg gggatgcgac cataatagat gatgagggta aagatataac catccaggat
     1981 atagacgaag gggataaggt taagatccac tatgacgggc agatagccga aagctatccg
     2041 gcgcagatcc ataggtgtca tagaattgtt ttggtaaaat agatttatta gaaggggggc
     2101 atttatatgt gcggaaggtt tctgctggat acggagataa gggagataat taggacatat
     2161 aagatacaga aaaatagaaa tacggattac aagtccggag agatattccc ttctacaaat
     2221 gcccccatcg tatttgaaga tggggaaaga accatcgcct cggccaaatg gggatttccc
     2281 tatgggttta aaaagggtat agtaataaat gcccgttcag agaccattat gcaaagacct
     2341 atgtttaaaa actccttcta taccgctaga tgcgttatcc ctgctaacct atattacgaa
     2401 tggaaggatg aaggcggaaa gaggaaggta aggcatggga taggtgttca cgatatggac
     2461 cttatttccc ttgggggaat ttataaggta tccctggatg aaaacggctt aatggagcag
     2521 atgacttttg ttataatcac cgcaaaagct gatacagcta taaggagtat acataaccgt
     2581 atgcccctta taatagggga tagggatatt gaaccatggc tggataaggg tgccgatata
     2641 aagtatattg aggatattct aaagcctaaa atggattata gcttctccat cagaaggttt
     2701 gaagataaat ccgctgaaga tagggacaat gaggatcacc agcaattgaa gatgttttga
     2761 tgatattaga atttatagga ttaaaaaggt gagcaacgag ggtttgtccc tgtgctcacc
     2821 tttttatgct tcggctgatg cttttgatgc cctagaaggg gttgatcctt ctgactattt
     2881 cttctatctc gttggtgaat cccgataagg gtttgccgcc ttctatatcc ttggccatgg
     2941 tcttaatcct gctcagaagg tcggggtctg cggtaacaac aacctttttg atggtggatt
     3001 ccaatttctt aacccgcctg tccactgatt ttttgatggc atccgttacc ttacccttat
     3061 actgtttttc aaactgcaat cccaccaatg ccgtatcgcc tgtcacaaca cagcttgccg
     3121 attggatgtt cttctccttg gctacatccc tggctatggc cttagccctg tcgcctgcgg
     3181 tgttggttgt tgagggccgt cgtaccgttc caggggtcgt cggtccgggg gttgtcgttc
     3241 ggggtgtccc cggcgtcatg gttcccggtc ctccgggggt catgattccg ttagtgcccg
     3301 gagtgacggt tcccggatcg ttagggttgg tggtgcgggg cgttaccctg gtgggagccg
     3361 ggaccggtct ttggataccg ggtgcggggg tcgtaggcgg cgttaccctg gtcgggcccg
     3421 ggaccgtcct ggtctgcccg ggtgccgggg taatggggcg cctattagta gtacaaccga
     3481 aaagtgcaat ggtgctgatt aataataaga cgcttaataa tacccgtatt ctgtttttca
     3541 aattacacta cctccttgct acaatttgat aaaaataata tagtttctgg cccctgtata
     3601 gaaaaaactg tacaccgcac tttaaatgcc taaaaaatta taggaatttt ttaagactta
     3661 atgcttttat agtttctaca taagttaata aatat
//
LOCUS       DASRNX010000079         7847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_493400,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000079 DASRNX010000000
VERSION     DASRNX010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493400"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..2279)
                     /locus_tag="VFD89_03165"
     CDS             complement(117..2279)
                     /locus_tag="VFD89_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZJ57221.1"
                     /translation="MRNKAEHNTKRDLSKNKRATQSNGVKRSKIHSIRLKLVASFLSL
                     ILAIIILGIASHGLASRKIEDITQNFTVQTMDQTKRYLDLIFDEVDGISTQFFFDAPI
                     QDYYLQYFLLKKDGSIDHKDEIMRTDIQKSLNSTVSAYEFINSIIILTDEKTSFTTCN
                     TPFDEVDWSQIETMDWYIQAMENQGKGSWIGDHSEIDVLFSSSGHRDYAFSYVREFKH
                     VESSSKLGILIVDIDKRAVENLLSGIQIGKSGEMHLITPSGDDLTPGIQQTTDEDQAE
                     EQLTFTQQPIFDQILESDKESDHIYGEYMGSEHLVMYNKVEGTGFILVSLIPKSELLE
                     ETRQIQQYTVVMVLGAGLFALFLGLYISTNMGRTINRLVDVASLAAEGDLTLEPKSRR
                     KDELGILTSSIRMMMASTRQLIERATLISQRVLDTSSTVASTSEEVSESSNDITRAIQ
                     EISQGATEQAMEAEKGVITMEQLASTINLVMDDARTIGDVSKDTMGLTQQGLSSINNL
                     NEKSTETAEITKNIIGSIQELNQHSLSINKIIKVIDGIADQTNLLALNAAIEAARAGD
                     MGSGFAVVANEVKKLAEQSIKSTNEIAAIIKTTQERTAVTVQYAKTAGDIVETQNEAV
                     RAAVSVFDRISSSMDTLSHLINEILIRITEVDSNKNQAIESMQNISAVSEEAAASVQE
                     VTASTEEQLAGIEELTAFAQELNDVASQLNGAINRFQV"
     gene            2822..3511
                     /locus_tag="VFD89_03170"
     CDS             2822..3511
                     /locus_tag="VFD89_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZJ57222.1"
                     /translation="MSNQGVRVMVVDDDPNIGQLIQLYLEKEGYETEYYADGISGLEG
                     FRKNPPHLIILDIMLPKMDGWDVCKSIRRTSQIPIIMLTAKGETFDKVLGLELGADDY
                     MVKPFDPKELVARVKAVLRRSKGPKDDEELISYPNLTINLTDYTLTFMGKDIECPPKE
                     LELLYFLASNPNKVFTREQLLEQVWGYDFYGDSRTVDVHVKRLREKLALDESSWGIKT
                     VWGVGYKFEVK"
     gene            3511..4938
                     /locus_tag="VFD89_03175"
     CDS             3511..4938
                     /locus_tag="VFD89_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ57223.1"
                     /translation="MFRSLFSRLMVTYFIIMLVTLITLGLFLSGALQKHTLKSTEGEL
                     IREARELNRHYELYDKGWVSREYLQLISDGITRYDKTSIWVVRVVGDIGLLQFQYTPQ
                     GINEEIEVTPFSKDEIINVLKGNMIKNIGKFGERFSVPVLTIGVPLELDQKIKGAIFF
                     HTPVEEIDLILYRLYGDIWKAVIFSATLSVVLLYWISRRISKPLSHMNEVSRQIAGGN
                     YDRRVKVSNRDETGQLAISFNAMADSLERLEDMRRSFVANVSHELRSPLTSIRGYIQG
                     ILDETFNPREQKKYLGIALEETQRLNKLINELLDLSQIESGQFPLNITGFDINEQIRL
                     LLIAQEDRINAMEMDIRVDFQEDRCLVNADIDRIKQVIINLLDNAIKFNRRGGVLLIK
                     TWKTGSKVFVKIQDQGPGIPKDEVKSIWDRFYQVEKSRSGTERGTGLGLSIVKKIVDE
                     HHQGIWVHSKEGQGTAFIFSLEAGS"
     gene            5052..6251
                     /locus_tag="VFD89_03180"
     CDS             5052..6251
                     /locus_tag="VFD89_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZJ57224.1"
                     /translation="MNDFYGFEEGSHPRRGGFWKYTAVALVFLLIGALGMYYIPGIGK
                     TIRDTGGQDIGITLHDKAVPEKDIDEPESNPDPIPALGAKGDLFIKSDNPVIEIAEKV
                     GPAIVGITNKSIITQTDPFWGGTTEREREGYGSGIIISEDGYIVTNNHVIDNASELVV
                     ILKGGKEVKATLIGSDSITDVAVIKIDEPDLTVAKIGDSEKVRQGELAIAIGNPLGHT
                     LAGTVTVGVISAVDREINFGQHQFTMIQTDAAINPGNSGGALVNSNGEIIGMNTLKTY
                     AEGLGFAIPTNVFVPLAEELIKSGSIQRPGIGVIIREITDELRETYGYPKGVGIIEIA
                     PNGAADTAGLLPKDIIIGVEDSTIEDIEELRKGINKHKVGDIIEFTIWRNGKTFIAPI
                     KLQQLQG"
     gene            complement(6314..>7847)
                     /locus_tag="VFD89_03185"
     CDS             complement(6314..>7847)
                     /locus_tag="VFD89_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57225.1"
                     /translation="YYCGHRLKKDDKSILGVRGDGADIHNQTTKPEEPTKPVEHEITI
                     EEPCTEAPANDKHNHDYSNLFGDNDLFRASDAFTGGSMPEIDLGTQIPLRRYQKDKRQ
                     AGSGRLLKVVFPVLTLILGLLAVFYWMSQTGRRFPGTNNLPQTIAVSTSVEQITKDDE
                     KAYRIVFNTVNGKEISFLNDIKAVENGRAEFIIDEAGLYGYSPQLDEEGSYQVYLDAT
                     VSAPNLPETMERVSIALSEPYNYAPFTLLMPSSSETEFQGDSSKVSFRIQPDSNVLIN
                     GEDFSETVSKDGRFEMEFHLPPKQDELVLDIRVSTPGYLDNIQQIILRRAHLDVTLSI
                     NETSPILSDEQWVKITGVIHPEASLETDVEIFEEPDIDSSTGAFSAYIKADRPGYTPC
                     TLTAKLGDKESSIEIIVDRKTTVDAYTSTAWKPDYIKLQQDDQLNNGRHFVFSGNVVE
                     IIESGHKNVFTVSEPQSDRLYHVEFWGSFDFNPGEEIRVFGNRWGNKEGLPRFLAKYI
                     YR"
BASE COUNT         2163 a         1597 c         1851 g         2236 t
ORIGIN      
        1 tattaatgat gcccattatg gtaatattgg gtattctgga ttaccggcag tcggcatccc
       61 cctgacatca aaccatcaaa aatacaatag ctataaagat ccatcccgtg gtgcttttat
      121 acctgaaacc tgttgatggc accatttaat tggctcgcta catcgttgag ctcctgtgcg
      181 aatgctgtta actcttcaat accggccaat tgctcctcag tggatgcagt cacttcttgg
      241 accgacgctg cggcctcttc cgatactgcg gaaatgtttt gcatggactc tatcgcctgg
      301 tttttattag aatctacttc ggtaattcta ataaggattt catttataag gtgagaaagg
      361 gtatccatgg atgatgatat cctatcaaaa accgataccg cagccctaac ggcctcattt
      421 tgagtttcga caatatcgcc ggcggtcttt gcgtattgta cggtgacagc tgtccgttcc
      481 tgagtagttt ttattatcgc cgctatttcg ttggtggatt taatggattg ttcagctaat
      541 tttttgacct catttgctac tacggcaaac cccgatccca tatcaccggc gcgggcagcc
      601 tctatggcgg catttagcgc caagagattg gtctgatcgg ctattccatc tataaccttt
      661 attattttat taatggagag cgaatgctga tttaattcct gtatagaccc aataatattt
      721 ttagtgatct ccgccgtttc ggtggatttc tcatttaggt tattaattga ggatagcccc
      781 tgttgagtaa gccccatggt atccttagag acatcgccga tggtccgggc atcatccatt
      841 acaagattga tagtagaagc taactgttcc atagtgatta cacccttctc cgcttccatg
      901 gcctgttccg tagcgccctg ggaaatctcc tgaatagccc ttgtgatatc attggaagat
      961 tcagatactt cctcggaggt gctcgctacg gtagaagagg tgtcaagtac cctttgcgag
     1021 attaaagtag ccctttcgat tagctgtctt gtgcttgcca tcatcatcct aatgcttgag
     1081 gtaaggatcc ccagttcatc tttccgcctg gacttaggct cgagggttaa atccccctct
     1141 gcggcaaggc tggctacgtc aaccaaacgg ttgatggtac gccccatgtt ggttgagata
     1201 tataaaccga gaaatagggc gaaaaggcct gcgccgagga ccattacaac ggtgtattgc
     1261 tgaatctgcc gggtttcctc taataattcc gatttcggaa taagggagac caggataaaa
     1321 ccggttcctt ctaccttatt gtacattacc aggtgctcgg aacccatgta ttccccatat
     1381 atatgatcgc tttccttatc actttctaga atttgatcaa aaataggttg ttgggtaaag
     1441 gttaattgct cttcggcctg atcctcatcg gtggtctgtt ggattcccgg ggttaaatcg
     1501 tctccgctag gtgttatcaa gtgcatctca ccggattttc ctatctgtat gcctgagagg
     1561 aggttttcaa cggctctttt atcgatatct acaataagga tccccaattt ggaggagctc
     1621 tccacgtgct tgaattctcg tacatatgaa aaggcataat ccctatgacc acttgaggaa
     1681 aacaatacat caatttcgct gtgatcccca atccatgagc ctttcccctg gttctccata
     1741 gcctggatat accagtccat ggtttctatc tgggaccaat ctacctcatc aaatggggta
     1801 ttacatgtgg taaaggatgt cttctcgtcg gtcagtatga taatactatt gataaattca
     1861 taggccgata ccgtgctgtt gagagacttt tggatatccg tcctcatgat ttcatcctta
     1921 tgatctatgg agccatcctt ttttaggaga aaatactgca gataataatc ctgtattggg
     1981 gcatcgaaga aaaactgggt ggatatgcca tcgacctcat caaaaattag gtccagatac
     2041 cttttagttt gatccatggt ttggacagtg aaattttggg ttatatcttc tatctttctt
     2101 gaagccagcc catgggatgc aatacccaat attataatag ccagaatcaa tgataaaaac
     2161 gaggcaacca gcttaagcct aatgctatgt atttttgatc gctttacacc gttggattgg
     2221 gttgcccttt tatttttaga aaggtcacgc tttgtattat gttccgcttt attccgcatt
     2281 gagagtcctc ccatttctta agatatggtt gatatccaga tgacaagacg aatcaaggtt
     2341 gtatacaata ccaaatttga acgggatatt aaagctttgg atattgtgta cggttccgca
     2401 tcatgatgtc tctggggtga aactgggcta caatggaaaa cgcaataaaa tgattacttt
     2461 aaaacatcgg aagcaattgc attggcaatt gcaacacagg tttcaacgag gcgagtgatt
     2521 tatacccacc gcttgtttta ctaatcggga ttcgccactt atagaagcgg gagacatctt
     2581 tcttcctcgt tataatctat aattctacaa aaatccaatt aaccctcttt tttatatgtg
     2641 gatttcgcaa aatttgcagg gatgatatag ccgcccgcaa tctcattcct catcataggg
     2701 gcatctgcgg ccaacctcta ttttcaattg attttgaatg ggcggctttg gcttttcaaa
     2761 acacgctttt gacgatataa ttaatcttag atagccatat ttgtgaaagg gattgataaa
     2821 tatgagtaac caaggtgtga gggttatggt tgtggacgac gatcctaata tcggccagtt
     2881 aatacaactg tatcttgaaa aggaaggtta tgaaactgaa tattatgcgg atgggatatc
     2941 gggactggaa gggtttagaa agaatccacc ccaccttata atactggata taatgttgcc
     3001 taagatggat ggttgggatg tatgcaagag catccgaagg acaagtcaga tccccatcat
     3061 aatgcttact gctaaagggg aaacctttga taaggttctg ggacttgaac taggagcgga
     3121 tgattatatg gtaaaaccct ttgatcctaa agagttagtg gccagggtta aggccgtact
     3181 cagaagatcc aagggcccaa aggatgacga agaattgata tcgtatccta accttaccat
     3241 caatctcacc gattacaccc taacatttat gggaaaggat attgaatgtc ccccaaagga
     3301 actggagctg ttatatttct tggcatcaaa ccccaacaag gtcttcacca gggaacaatt
     3361 attggagcag gtatggggtt acgattttta tggggattcc agaaccgttg atgtgcatgt
     3421 aaaaaggctg agggagaaat tggctttgga tgagagcagt tgggggataa aaacagtatg
     3481 gggtgtaggc tataaatttg aggtgaaatg atgtttcgat ctttattttc acgcctgatg
     3541 gtcacctatt tcataattat gcttgtgact ttaataacct tgggactgtt cttatcaggt
     3601 gctttacaaa aacacaccct aaaatctacc gagggagaat taattcgtga ggccagggag
     3661 cttaaccggc actatgaatt atatgataag gggtgggttt ctcgggaata cctgcagctt
     3721 ataagtgacg ggataactag gtatgataag actagcattt gggtggtaag ggttgtcggg
     3781 gatataggtc tcctacaatt tcaatataca ccccagggaa taaatgagga aattgaggta
     3841 accccttttt ccaaggacga gataatcaac gttttaaagg gcaatatgat taaaaatata
     3901 ggaaagttcg gggagcgctt ttcggtacct gtattaacca tcggggtgcc gcttgaactg
     3961 gatcagaaga ttaagggggc catattcttc catacccccg ttgaggaaat cgacctgata
     4021 ctatatcggc tgtatgggga tatctggaag gctgttattt tttctgcaac cctgtccgtc
     4081 gtgctcttgt attggatttc caggaggata tccaaaccct tgagtcatat gaatgaagtc
     4141 tccagacaaa ttgccggcgg taattatgat cggagggtta aggtgtccaa tagggatgaa
     4201 accgggcagc tggccatcag ttttaatgcc atggctgatt cccttgagag gctggaggat
     4261 atgagaagga gctttgtagc aaatgtatcc catgagctta ggtcccccct aacttccatc
     4321 agggggtata ttcaggggat attggacgag accttcaacc caagggagca gaaaaagtat
     4381 ctgggtatag ctctggagga gacccagcgg cttaacaagc ttataaatga actgttggat
     4441 ctgtcacaga tagaatcggg acaatttcca ttaaatatta cgggatttga tatcaacgag
     4501 cagataagac tccttttgat agcccaggag gataggatta atgccatgga aatggatatc
     4561 agagtagatt ttcaggagga taggtgcttg gtaaacgccg atatagaccg tatcaagcag
     4621 gtgattatta atctccttga caatgcgata aaattcaatc gacgtggggg tgtactcctt
     4681 atcaagacct ggaagaccgg aagcaaggtc tttgtaaaga tccaagatca ggggccaggg
     4741 atacccaagg acgaagtcaa gagtatatgg gacaggttct atcaagtgga aaaatcccgc
     4801 agcggtacgg agcgagggac cgggctgggt ttatccatag taaagaagat agtggatgag
     4861 catcaccagg ggatctgggt gcacagtaag gagggacagg gaactgcttt tatattctca
     4921 ctggaggctg gttcctaagc accggacgga tagctatgat aaatagcatt tagaaattgt
     4981 ttacacacag ttcatattct cttcaaaaaa caagtttata atatatgcat aaagagtcgg
     5041 aggtgaagtt tatgaatgat ttttatggat ttgaagaagg gtctcatccg agaaggggtg
     5101 gattttggaa gtatacggcg gtggcgttag tatttttgtt aataggagcc cttggcatgt
     5161 actatatacc cggtatcggc aaaaccataa gggatacagg aggccaggat atcggcatta
     5221 ccttacatga taaagctgtg ccggaaaagg atatagacga accggaatca aacccggatc
     5281 ccataccggc cctgggggca aaaggggatc tgttcattaa atcggataac ccggtgatag
     5341 aaatagccga aaaggtgggt cctgctattg tagggataac caataaaagt ataataacac
     5401 agacggatcc cttctggggc ggaaccaccg aaagggagag ggaaggttat ggctccggaa
     5461 taatcataag tgaagatgga tatatagtga caaataacca tgttattgac aatgcaagcg
     5521 aattggtcgt tattctaaag gggggcaagg aagtaaaggc aaccctaatc ggaagcgatt
     5581 caatcaccga tgttgcggta atcaaaatcg acgagccgga tcttaccgtg gcaaagatcg
     5641 gggattccga aaaggttcga caaggtgaac tggctatagc catcggtaat cccttgggac
     5701 atacattggc agggaccgtt acggttgggg tcattagtgc cgttgatagg gagataaact
     5761 ttggacagca tcaatttact atgattcaga ccgatgcggc tatcaatccg ggcaacagcg
     5821 gcggggccct tgtaaattct aacggtgaga ttataggtat gaacactttg aagacatatg
     5881 cggaggggct gggatttgcc ataccaacca atgtgttcgt ccccttagcc gaggaattga
     5941 taaagagcgg aagcattcag cggccgggga taggagtaat tatcagagag ataacggatg
     6001 agttgaggga gacctatggt tatcctaagg gagtgggcat aatagagatt gcacctaacg
     6061 gtgcagccga tacggcgggc ctgttgccca aggatataat aataggcgta gaggatagta
     6121 ccatagagga tattgaggaa ctgagaaagg gtatcaacaa acataaggtc ggagatatta
     6181 tagaattcac catatggcgt aatggaaaga ccttcatagc cccaatcaag ctccaacaat
     6241 tgcagggata aaaagccagt taatatctgg ctttttttct caatactcag cggggatatt
     6301 caataatccc tagtcagcgg tatatgtatt ttgctaaaaa tcgggggagg ccttccttgt
     6361 tgccccatcg atttccgaaa accctaatct cttcgccggg attaaaatcg aaactacccc
     6421 aaaattccac gtgatagagt cggtccgact ggggttcgct tacagtgaag acgtttttat
     6481 ggcctgactc tattatctcg actacattac cgctgaacac aaaatgcctc ccgttgttga
     6541 gctgatcatc ttgctgcagc tttatataat ccggtttcca agctgttgaa gtgtacgcgt
     6601 cgactgtggt ttttctgtcc actataattt ctatggagga ttctttatcc cccagtttcg
     6661 cggttaatgt acagggggta taccccgggc gatcagcttt tatataggct gagaaagcgc
     6721 cggtactgct gtctatatcc ggttcctcga atatttcgac atcagtttcc aggctcgcct
     6781 caggatggat aacgccggtt attttcaccc attgttcatc ggatagtatc ggcgaggttt
     6841 cgttgatact gagggtgaca tctaaatgtg cccttctaag gatgatttgt tgaatattat
     6901 ccaaatatcc cggggtagag acccggatat ccaacaccag ttcatcctgt ttggggggaa
     6961 gatgaaattc catttcaaac ctgccatcct ttgacacggt ttcagaaaaa tcttctccat
     7021 tgattaagac atttgaatcg ggttgaatcc taaatgaaac cttcgagcta tccccttgaa
     7081 attctgtctc tgaagaggat ggcatcaata atgtaaaggg ggcataatta tacggttcgg
     7141 agagggctat agataccctt tccatggtct ctggcaggtt aggagccgat acagttgcat
     7201 ccagatagac ctggtaactt ccctcctcat ctagttgggg actatagcca taaagccctg
     7261 cttcatcaat tatgaactct gcacgtccat tttctacggc cttaatgtcg tttaaaaaac
     7321 taatttcctt accattgaca gtattaaaga ctattctgta cgccttttca tcgtctttgg
     7381 ttatctgttc taccgatgta gacacagcaa tggtctgggg aagattatta gtccctggga
     7441 atctccgacc agtctggctc atccagtaga atacagccag aagtcctaat atcagggtta
     7501 agacggggaa gactactttc aagagccttc cagacccggc ttgtcgctta tctttttgat
     7561 atctccggag gggtatctgt gtgccaaggt ctatttccgg catactgcct cccgtaaatg
     7621 catcactggc tcgaaacagg tcattatctc caaataaatt gctgtaatca tggttgtgtt
     7681 tatcatttgc cggggcttca gtgcaaggtt cctctatggt aatttcatgt tccacaggct
     7741 ttgtcggttc ttcgggcttt gtagtctgat tatgaatatc tgccccgtca cccctaaccc
     7801 caagaatgga tttatcatct ttttttagcc tatggccgca ataataa
//
LOCUS       DASRNX010000080        15926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_439144,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000080 DASRNX010000000
VERSION     DASRNX010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15926
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439144"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /gene="tuf"
                     /locus_tag="VFD89_03190"
     CDS             <1..134
                     /gene="tuf"
                     /locus_tag="VFD89_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805135.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZJ57226.1"
                     /translation="MPGDNIDMEVELITPIAIEEGLRFAIREGGRTVGAGVVASVAK"
     gene            360..509
                     /gene="rpmG"
                     /locus_tag="VFD89_03195"
     CDS             360..509
                     /gene="rpmG"
                     /locus_tag="VFD89_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZJ57227.1"
                     /translation="MRVKITLACTDCKQRNYNTMKNRRNNADRLEMRKYCKFCKGHTM
                     HRETR"
     gene            582..812
                     /gene="secE"
                     /locus_tag="VFD89_03200"
     CDS             582..812
                     /gene="secE"
                     /locus_tag="VFD89_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZJ57228.1"
                     /translation="MSKGKSTARAAAKTRKPRKGIKKFFREVISEVKKVSWPNLKELT
                     NYTIIVVVLILIFAAAIGIIDFGLGKLFTLIS"
     gene            856..1386
                     /gene="nusG"
                     /locus_tag="VFD89_03205"
     CDS             856..1386
                     /gene="nusG"
                     /locus_tag="VFD89_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZJ57229.1"
                     /translation="MSENEKDQGQWYVVHTYSGYENKVKANLEKMVENRGLEDLIFEV
                     TVPMEEQVEVKDGKRKSVMRKMFPGYVIVKMIMTDESWYVIRNTRGVTGFVGPGSKPI
                     PLTDDEIRKLGVEDVPVELKAEVGDNIRVISGPLENFIGVIEEIHPEKQKVRVSVSMF
                     GRDTPIDLEYHQIQRI"
     gene            1438..1860
                     /gene="rplK"
                     /locus_tag="VFD89_03210"
     CDS             1438..1860
                     /gene="rplK"
                     /locus_tag="VFD89_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZJ57230.1"
                     /translation="MAKKVTGLIKLQIPAGKATPAPPVGPALGQHGVNIMGFCKEFNE
                     KTASQAGLTIPVVISVYQDRSFTFITKTPPAAVLLKKAAGIELASGQPNLEKVATVTR
                     EQVKEIAELKMPDLNAASVEAAMSMVEGTARSMGIVVK"
     gene            2180..2869
                     /gene="rplA"
                     /locus_tag="VFD89_03215"
     CDS             2180..2869
                     /gene="rplA"
                     /locus_tag="VFD89_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZJ57231.1"
                     /translation="MRRGKKYQDSLELVDRTKLYDTQESIELLKQTAKAKFDETIEAC
                     VRLGVNPRHADQQVRGAVVLPHGTGRSVRVLVFAKGDKAREAEGAGADFVGAEDLAEK
                     IQNENWLDFDICVATPDMMSVVGRLGRVLGPRGLMPNPKSGTVTMDVEKAVQDIKAGK
                     VEYRVDRMGIIHVPIGKVSFDTEKISENFSVLMDAIVKAKPASSKGTYLRSVVLSSTM
                     GPGIKVNPSLF"
     gene            3099..3620
                     /gene="rplJ"
                     /locus_tag="VFD89_03220"
     CDS             3099..3620
                     /gene="rplJ"
                     /locus_tag="VFD89_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZJ57232.1"
                     /translation="MSMRKQKGLVVQELKEKFTKSSSAILVDYKGLNVQEVTELRGNF
                     REAGVDYKVYKNTLTEIAAKEMGLEGLIPYLEGPTAIAFSEEDPVAPAKILTEAIRKY
                     RKIEFKGGVVDGKVIDIDGVKALAELPSREELIAKLLGSINAPIANLVGVLSGPVRAL
                     AFALNAIKEQKEA"
     gene            3667..4041
                     /gene="rplL"
                     /locus_tag="VFD89_03225"
     CDS             3667..4041
                     /gene="rplL"
                     /locus_tag="VFD89_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZJ57233.1"
                     /translation="MDKNQIMEAIKGMTVLELAELVKELEEEFGVSAAAPMAVAAGPA
                     AGPAAAEEEEQTEFDAVLAEIGAQKIKVIKVVREITGLGLKEAKDVVDSAPKAVKEGV
                     SKEEAEEIKAKLEEVGAKIEIK"
     gene            4544..8242
                     /gene="rpoB"
                     /locus_tag="VFD89_03230"
     CDS             4544..8242
                     /gene="rpoB"
                     /locus_tag="VFD89_03230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZJ57234.1"
                     /translation="MVHPVKMGKKTRMSYSRIDEVLEMPNLIEVQKRSFWWFLKEGLR
                     EVFNDISPISDYTENLVLEFVDYQLGDNPKYSVEECKERDVTFSAPLKVQVRLINRET
                     GEVKEQEVFMGDFPLMTDQGTFIINGAERVIVSQLVRSPGIYYSDIIDKTGKNLYSAT
                     VIPNRGAWLEFETDSNEILYVRVDRTRKLPITVLLRALGIGTDAQIKAIMGEDERLLK
                     TLDKDNTTSHEEGLLEIYKRLRPGEPPTIESARQLFNLLFFDDKRYDLARFGRYKFNK
                     KLALASRIAGRKSADDIINTETGEILAQKGERISYEKAWEIQNMGINQVVLDLDEDKT
                     AHILGNNFVDAKYHLDLDLEEIGINEHVHYPTLREILEQDLDEKELKDALVENINRLI
                     PKHVIVDDIAAAINYIIYLLHSVGHVDDIDHLGNRRLRSVGELLQNQFRIGLSRMERV
                     VKERMTIQDVDIITPQALINIRPVTASIKEFFGSSQLSQFMDQTNPLAELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLIGSLSTYARINEYGFIEAP
                     YRRVDKEKNIVTDEIIYLTADEEDEFTIAQGNEPLDDNGHFVDERVMARVKDDIYEVD
                     RNNVEFMDVSPKQVVSVATAMIPFLENDDANRALMGANMQRQGVPLVRTEAPIVGTGM
                     EYKAARDSGVVLIAVEDGTVIRLSATSIHIRNHKGEVTDYKLHKFKRSNQGTCINQKP
                     IVEIGEEVKKGQIIADGASTDNGEIALGKNVLMGFMTWEGYNFEDAILISEKLVKDDV
                     YTSIHIEEYESDARDTKLGPEEITRDIPNTGEEALSDLDERGIIRIGAEVRANDILVG
                     KVTPKGETELTAEERLLRAIFGEKAREVRDTSLRVPHGEGGIVVDVKVFTRENGDELP
                     PGVNELVRVYVAQKRKISVGDKMAGRHGNKGVISRILPEEDMPFLPDGTPLEIVLNPL
                     GVPSRMNIGQVLEAHLGMAAKALGWEIVTPVFDGANEVDIMDILEQAGLQRNGKVTLY
                     DGRTGEPFDSEITVGYMYILKLAHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFG
                     EMEVWALEAYGAAHTLQEILTVKSDDVVGRVKTYEAIVKGENIPEPGVPESFKVLIKE
                     LQSLALDVKVLSEESREIEILEDYSDEYDELDVNIEGRETTEEEDGEEDLEDFDLDSI
                     IDIPDLSDTLFNDLDELTDTDDDDDDIE"
     gene            8260..11826
                     /gene="rpoC"
                     /locus_tag="VFD89_03235"
     CDS             8260..11826
                     /gene="rpoC"
                     /locus_tag="VFD89_03235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZJ57235.1"
                     /translation="MFELNNFDSIQIGLASPDKIREWSRGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPQKDWECHCGKYKRVRYKGIICDRCGVEVTRSKVRRERMGHIELAASVS
                     HIWYFKGIPSRMGLILDISPRSLEKVLYFAAYIVIDPGETSLLKLQLLTDREYRENYD
                     KFGNEFKAGMGAGAIKEILEGLDLDKMSRELKEELKTATGQKRIRVVRRLEVVEAFRK
                     SGNRPEWIILDVIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKMYQCGLPKEMALELFKPFVMKRLVEKGLAHNIKSAKRMVEK
                     VKAEVWDVLEEAITEHPVLLNRAPTLHRLGIQAFEPVLVEGRAIKLHPLVCSAYNADF
                     DGDQMAVHVPLSVEAQSEARFLMLSTNNILKPSDGRPVVSPTQDMVIGIYYLTSLKED
                     SKGKGKLFISPDEVIMAYGAGVIDLHAPIKMRVEREIDGKKEARLIDVTAGRVIFNEA
                     IPQDLGFRDRTNPDELFELEVNEVVDKKVIGRIVDRCYRKYGTSGTSIVLDKLKDLGF
                     HYSTIGAVTVSVSDIVIPEEKQGLLEKADQLVLGVDRQYRRGLISDTERYERVINTWN
                     KTIDDVTDALMGSLDHFNPIYMMANSGARGSINQIRQLAGMRGLMANPTGKIIELPIR
                     ANFREGLTVLEFFISTHGARKGLADTALRTADSGYLTRRLVDVSQDVIVREGDCFAKT
                     GQKPQGILVDEIKDADEVIEPFIDRITGRYVAEDINHPETGEIIAMANERIDHDLAEE
                     IVRAGITKANIRSVLTCRTEHGVCAKCYGSNMASGEPVNIGEAVGIIAAQSIGEPGTQ
                     LTMRTFHTGGIAGADITQGLPRVEELFEARKPKGLAVISEISGIVKINETKKKREVSI
                     MGADGDTKNYTIPYGSRISVVEDQQVEAGDELTEGSINPHDILKIRGVKGVQSYLLHE
                     VQKVYRMQGVDINDKHIEVIVRQMLKKVRIEDAGDTDMLPGGLIDIFEFERENDKVVE
                     EGGQPAFAKRVLLGITKASLATESFLSAASFQETTRVLTEASIKGKSDPLVGLKENVI
                     IGKLIPAGTGMSRYKDIEVYTEEEPEEIIPVDIEEEEPVLQA"
     gene            12448..12702
                     /locus_tag="VFD89_03240"
     CDS             12448..12702
                     /locus_tag="VFD89_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HZJ57236.1"
                     /translation="MLEGLREASNKTVGMKQTLKAVQGGLALKVFLAGDIDDYISQKI
                     RHECQRHDIDIIMVDSMKELGEACGISVGAATAAILEVLD"
     gene            12742..13116
                     /gene="rpsL"
                     /locus_tag="VFD89_03245"
     CDS             12742..13116
                     /gene="rpsL"
                     /locus_tag="VFD89_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZJ57237.1"
                     /translation="MPTISQLIRKGRKSIGHKSDAPALKGSPQKRGVCTAVSTRTPKK
                     PNSALRKLARVRLSNGMEVTAYIPGIGHNLQEHSVVLIRGGRVPDLPGVRYHIVRGAL
                     DTAGVTDRQQARSKYGAKKPRK"
     gene            13154..13624
                     /gene="rpsG"
                     /locus_tag="VFD89_03250"
     CDS             13154..13624
                     /gene="rpsG"
                     /locus_tag="VFD89_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZJ57238.1"
                     /translation="MPRKGHVPKRDVLPDPLYGSKVVTKLINQVMLDGKRGIAQRICY
                     GAFDIIRDKTGNEPIEVFEQAMNNIMPVLEVKARRVGGATYQVPMEVRTERRQTLGLR
                     WLVSFARNRGERTMIDRLASEIMDAANNMGGAVRRKEDTHKMAEANRAFAHYRW"
     gene            13710..15776
                     /gene="fusA"
                     /locus_tag="VFD89_03255"
     CDS             13710..15776
                     /gene="fusA"
                     /locus_tag="VFD89_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZJ57239.1"
                     /translation="MPRQFSLENTRNIGIMAHIDAGKTTTSERILFYTGRVHKMGETH
                     EGSATMDWMAQEQERGITITSAATTAQWKGYRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAIFCAKGGVEPQSETVWRQADKYRVPRIAYINKMDIIGADYFNVVEMMKERLGANAV
                     PLQIPIGKEDYFKGIIDLVERRAIYYTDDLGTHSEETDIPEDMADLVQEYRDILIEAV
                     AENDEDLMMKYLEGEELTPDEIKAGIRKATVSVDMIPVLCGSSYKNKGVQPLLDAVIE
                     YMPSPLEVAAIKGTIPGTDEETERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTL
                     KSGSYVYNSTKGKRERIGRILLMHANHREEVDEVYSGDIAAAIGLRDTTTGDTLCLEV
                     EPVILESMEFPDPVIQVAIEPKTKVGQDKMGVALQKLAEEDPTFRTYTDGETGQTIIA
                     GMGELHLEIIVDRLLREFKVEANVGKPQVAYRETIRKAVKSEGRFVRQSGGRGQYGHV
                     WLEVEPLEAGSGYEFVNKIVGGVVPREYVAPVDQGIQDAMASGILGGYPVVDIKATLY
                     DGSYHEVDSSEMAFKIAGSIAFKDAMRKANPVLMEPTMKVEVTVPEEYMGDVMGDINS
                     RRGRIEGMEAKSGVQVITSYVPLSEMFGYATDLRSKSQGRGVYTMHLSHYEEVPKSIA
                     EKIVAK"
     gene            15816..>15926
                     /locus_tag="VFD89_03260"
     CDS             15816..>15926
                     /locus_tag="VFD89_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZJ57240.1"
                     /translation="MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITMVLS"
BASE COUNT         4776 a         3135 c         4294 g         3721 t
ORIGIN      
        1 taatgcccgg ggacaacatc gacatggaag tggaacttat cacccccatc gccattgaag
       61 agggcctaag gttcgctatc cgtgaaggcg gcaggaccgt gggtgccggc gttgtagcca
      121 gcgttgcaaa gtaaatattg acagagtggg acgacctata atcgtccaaa tgctattttg
      181 atcagacgac ctaagagact ggccatatct gccagtctct tatttgaatg tcgtcaaaaa
      241 ggcgtccggt cagacggtgc ttcaaaaacc tgccgcaatg gacgggcaga aatttgttga
      301 caccaatgac catatatgat aaattaatta ggtatttaca cgtaaggtag gtgttctgtg
      361 tgagagtaaa gataacattg gcttgtacgg attgtaagca gcgcaactat aacaccatga
      421 agaataggcg aaacaatgct gaccgtctcg aaatgaggaa atattgcaag ttctgcaagg
      481 gtcatacaat gcatagggaa acccgataga agatatccat ccattgggaa gacttggtat
      541 aacatcgatc aatatggctt agaataagga tgtgaatcat tatgtcaaag ggcaaatcca
      601 cagccagggc agcagcaaaa acaaggaagc caagaaaagg aataaaaaag tttttccgag
      661 aggtcatatc cgaggtaaaa aaggtatctt ggccaaatct taaggaactt acaaattata
      721 ctattatcgt agtggttttg attctgatct ttgctgccgc tattgggatc atagattttg
      781 gactggggaa actgtttaca ctgatttctt gattaaggag gtaggggtca ggaagaaaag
      841 cttggctctc aggttatgtc tgaaaatgaa aaggaccagg ggcaatggta cgtggtccac
      901 acttattccg gatatgagaa taaggtaaag gctaatctcg aaaagatggt ggagaaccga
      961 ggcctagaag atctgatctt tgaggtcaca gttcccatgg aagaacaggt cgaggtcaaa
     1021 gatggcaaga gaaaatctgt catgcggaag atgtttccgg gttatgtaat agtaaagatg
     1081 atcatgacgg atgaatcctg gtatgtaatt cgtaatactc ggggtgttac cggttttgta
     1141 ggaccgggtt ctaagcccat cccccttacc gatgacgaga taagaaaact gggtgtggaa
     1201 gatgttccag tagagctaaa ggctgaagtt ggtgataaca tccgggttat atcaggaccc
     1261 ttggaaaact ttatcggagt tatcgaagag atacatcccg aaaaacaaaa ggtacgggta
     1321 tctgtatcca tgtttggaag agacacccca atcgatttgg agtatcatca gatacagaga
     1381 atttgatgga agacatcatt gtttatccat aactacacta cttgaggagg tgctaatatg
     1441 gcaaagaagg ttaccggact tataaaactt cagatcccgg cagggaaggc aactcccgct
     1501 ccacccgtag gcccggcttt gggtcaacac ggcgttaaca taatggggtt ttgtaaggaa
     1561 tttaatgaaa aaactgcaag tcaagcgggt cttaccattc ccgtagttat ttcagtttat
     1621 caagatcgct cttttacttt tattacaaag actcccccgg ctgctgtcct gcttaagaag
     1681 gctgccggta ttgaattggc ttcaggtcaa cccaatttag agaaggttgc taccgtgacc
     1741 cgggaacagg tcaaagaaat tgctgagctt aagatgcctg atttgaatgc cgccagcgta
     1801 gaagccgcaa tgagcatggt tgaaggaacc gccaggagca tgggaattgt tgtgaaatag
     1861 ggtattcaca ataaggttta aaactacatc atccaatata ttagggcaca acgttgataa
     1921 ttcaattctt ttaaaacctc aactggccgg ttatttattt gatgatatat atgttacgaa
     1981 aaagaccttt caatgtaaaa ccggtaaaat tatccgattt tacgttaaaa taatgtattt
     2041 tccgtaacat acgagttaag gataatgttt tacgacacct atataccaat gtaaaatgtt
     2101 tgtcgtaaaa catatagcta attaattatt atgtgggaga aaggtaatac tttcgcttgt
     2161 accacaagga ggcaataaaa tgagaagagg taaaaagtat caagatagcc ttgaactggt
     2221 ggatcgtaca aaattatatg atacccagga aagcattgaa ttgcttaaac agaccgctaa
     2281 ggctaaattt gatgaaacta tagaagcttg cgttagatta ggcgtaaacc ccaggcatgc
     2341 ggatcagcaa gtcaggggag ctgttgtgct tccccatggg acaggtcgat ccgttagggt
     2401 tttggtgttt gcaaagggag acaaagccag ggaggccgaa ggggcaggtg ctgattttgt
     2461 cggtgcagag gatctggctg aaaaaattca gaatgaaaac tggttggatt ttgatatctg
     2521 tgttgcaact cctgatatga tgagtgtagt aggtcgcctg ggacgggttc taggtcccag
     2581 aggattgatg cccaatccca aatccggtac ggtaaccatg gatgtagaaa aagcggttca
     2641 ggatatcaag gccggtaagg ttgagtaccg ggttgataga atggggatca tacatgtccc
     2701 tattggtaag gtttcttttg acacggaaaa gataagcgag aacttttcgg tgcttatgga
     2761 tgccattgta aaggcaaaac ccgcttcatc taagggtacc tatctaagaa gcgtagtgct
     2821 ctccagtacc atgggtccgg gaattaaagt aaatccaagt ttattttagg gtttttagat
     2881 tgacaaccac aaaattctat agtagacttt taatgaatta aatagcttat acctttcttg
     2941 ccgtagacag caggcactgc aattactttt attgcggttt aaaccatgag cccgccgagg
     3001 tggaagagca aacaggataa ctgccctctt gccatctggt tttatgccga acggagagag
     3061 ggtattttaa tgtgattcaa gaggaggtgt gaccggctat gtctatgaga aaacaaaaag
     3121 gcctagttgt acaggagctc aaggagaaat ttacaaaatc cagcagtgct attttagtgg
     3181 attataaggg gttaaatgta caggaggtaa ccgagcttag aggaaatttc cgcgaggccg
     3241 gtgtcgacta taaggtatac aagaacacgt taaccgagat tgcggctaaa gagatgggtt
     3301 tagaggggct aataccttat cttgaagggc ctacagcaat tgcttttagt gaggaagacc
     3361 ccgtcgcccc tgccaagatc ctgacagagg ccattagaaa atataggaaa attgagttta
     3421 agggcggagt tgtagacggt aaggtaatcg atattgacgg agttaaggca ttagcagaac
     3481 ttccatctag ggaggagctt attgctaagc tattgggcag cataaatgct cccattgcca
     3541 accttgttgg ggtgctcagc ggtcctgttc gggcgctggc ctttgctctt aatgcgatta
     3601 aagaacaaaa agaggcttaa agattttaaa ttaaacaaat tatagtaaaa acggaggttt
     3661 ataaagatgg ataaaaatca aattatggaa gcaattaagg gcatgacagt cctagaattg
     3721 gctgaactgg taaaggaact tgaagaggaa tttggagtca gcgcggctgc acctatggct
     3781 gttgctgcag ggcctgctgc aggacccgct gccgctgaag aagaagagca aacagaattt
     3841 gatgctgttc tggccgaaat cggcgcccaa aagataaagg ttatcaaggt tgttagagag
     3901 atcaccggtc ttggtctaaa ggaagctaaa gacgtagtcg acagcgctcc aaaggctgtt
     3961 aaggaaggcg tcagcaaaga agaggcagaa gagataaagg ccaagcttga agaagtcgga
     4021 gcaaagatcg aaattaaata agcttaccaa agacatttta gaatgaagga ctattactag
     4081 tccttttttc tttatgttca ttccccagaa tcatctaccc atacaaaaaa caaggacgcc
     4141 ttaagcagat tccactacaa ttttcatacc gataaattta gtttaagtga ttgggatatt
     4201 atttttcaaa tttagattat acacccaaaa taaaaaacat gcttgacagg aaatatgtgt
     4261 tatggtatca tattaaattg cattaatgta tgtatttctg cctgtttgtg gtaaaaatat
     4321 atcggaaata atgataaagg tttattgttt aatcatgggg aataaagact atatattatg
     4381 gcaataataa acaataatgc tccttggcaa ttttgcccaa tgggtcttgg gtcaaggggc
     4441 ttttaatatt cagatattat caatgagata accgaccagt taatgtttta aaacatttga
     4501 attatcagca atggttataa attcttaagg ggtgagttgt tgcatggtac atccggttaa
     4561 gatgggaaaa aagactcgta tgagttattc cagaattgac gaggttctgg agatgcccaa
     4621 cctcatagag gtacagaagc gatcattttg gtggtttctg aaagaaggtc taagagaggt
     4681 gtttaacgac atttctccta tctccgacta tacggaaaac ttggtgttag aatttgtgga
     4741 ctatcagctg ggtgataatc ccaaatattc cgtagaagag tgcaaagaaa gggacgttac
     4801 attttcggca ccgctaaagg ttcaggtcag gctcatcaac agggagaccg gggaggtcaa
     4861 ggagcaggaa gtcttcatgg gagatttccc gttgatgact gatcaaggaa cttttattat
     4921 aaacggagct gagcgtgtaa tcgtcagtca gttggtaaga tcccccggca tatattattc
     4981 agatataata gataagaccg ggaagaatct atattcggct acagttattc ccaacagggg
     5041 tgcttggctt gaatttgaaa ccgactccaa tgagatcctg tatgtgaggg ttgatcgcac
     5101 ccgaaaactg cccataaccg tccttctaag ggcgttgggt atcggtacag atgcccaaat
     5161 caaggctatc atgggagagg acgaaagatt acttaaaacc ttggataagg ataataccac
     5221 ttcccatgaa gagggattgt tggagatcta taagcgcctc aggcccgggg aacccccgac
     5281 catagagagc gccaggcagc tcttcaactt gttgttcttt gatgacaagc ggtatgattt
     5341 ggcccgtttt ggaagatata aattcaataa aaagcttgcc ttggcatcca gaatagcagg
     5401 gcgaaagagt gcagatgata ttatcaacac tgagacaggg gaaatcttgg cccaaaaggg
     5461 tgagagaatc agctacgaaa aggcctggga aatacaaaat atgggtataa atcaggtggt
     5521 gcttgattta gacgaggata agactgctca cattttgggt aacaactttg ttgatgccaa
     5581 gtatcattta gatctcgatc tcgaagagat tgggattaat gagcatgtgc attatcccac
     5641 attaagggaa attttagaac aagaccttga cgagaaggaa ctgaaggatg cgctggtgga
     5701 gaatatcaac aggctgatac ccaagcatgt cattgtagac gacatcgcag cggctatcaa
     5761 ttacatcatc taccttctgc acagcgtggg gcatgttgac gacatagacc acctaggcaa
     5821 tagaagactt cgttccgtag gggaactcct tcaaaatcag ttccggatag gtctttccag
     5881 gatggagagg gtggttaagg agaggatgac catccaggat gtggatatca tcactcctca
     5941 ggctctcatc aatatcaggc cggtaactgc ctctatcaag gagtttttcg gcagcagtca
     6001 attatcccaa tttatggatc agaccaaccc gttggctgaa ctcacccata aacgccgtct
     6061 cagcgcgttg gggcccggcg gtttaagccg tgagagagcg ggctttgagg ttcgggacgt
     6121 tcatcactcc cactacgggc gcatgtgtcc catagagact ccggaggggc ccaatatcgg
     6181 gcttataggt tccctgagta cctatgccag aataaacgag tacgggttta tcgaggctcc
     6241 ctataggaga gtagataaag aaaagaatat tgtaaccgat gagataatct atcttaccgc
     6301 cgatgaagag gacgaattta ctatagccca gggtaacgaa cccttagatg ataacggtca
     6361 ctttgtagac gaaagggtta tggccagggt caaagatgat atctatgagg tggacaggaa
     6421 caacgttgaa tttatggatg tgtcccccaa acaggtcgtc tcggtggcca ccgctatgat
     6481 ccccttcttg gaaaatgacg atgccaacag agccctgatg ggcgccaata tgcagcgtca
     6541 gggggttccc cttgtaagaa cagaggcccc catcgtaggt acaggtatgg aatataaggc
     6601 cgcaagggat tccggggtgg ttctcatagc cgtggaagac ggaacggtca tcaggctctc
     6661 ggctacatcc atccatatca gaaaccataa gggtgaagtc acggactaca agctccataa
     6721 gtttaagaga tccaaccagg gtacctgtat caatcaaaaa cccatagttg aaattgggga
     6781 agaggtaaaa aaaggccaaa tcattgccga cggagcctcc acggataatg gggagatagc
     6841 ccttggcaaa aacgtcctta tgggatttat gacctgggaa ggctataatt tcgaggatgc
     6901 tatcctcata agtgaaaaat tggttaaaga cgacgtttat acgtctatcc atatagaaga
     6961 atatgaatcc gatgccagag acaccaagct gggccccgag gagataacca gggatatccc
     7021 caacaccggg gaggaagccc taagcgatct tgatgagagg ggaattattc ggattggggc
     7081 cgaagtaagg gccaacgata tattggtagg aaaggtaacc cccaaggggg aaaccgagct
     7141 caccgctgag gagagactcc ttagggcaat ttttggtgag aaggcacggg aagtcaggga
     7201 tacctccctt agggttcccc atggtgaggg cggaatagtt gtagatgtca aggtctttac
     7261 cagggaaaac ggtgacgaac tgcccccagg ggtaaatgaa ctggttaggg tctatgttgc
     7321 ccaaaagaga aagatctccg taggggataa gatggcgggg cggcacggga acaagggagt
     7381 tatttcaagg attcttcccg aggaggatat gcccttcctg cccgacggaa caccccttga
     7441 gatagtgcta aacccattgg gtgtgccctc ccgtatgaat ataggacagg tgctggaagc
     7501 tcacttgggt atggctgcga aagccctggg gtgggagatc gtcaccccgg tattcgatgg
     7561 cgccaacgaa gtggatatta tggacatctt ggaacaggcc ggacttcaaa gaaacgggaa
     7621 ggtaaccctg tacgacggtc gcaccggcga acccttcgac agcgaaatta ccgtagggta
     7681 tatgtatatt ttgaagctgg cacacttagt ggatgataag attcatgcaa ggtctacggg
     7741 accgtattca ttggtaaccc agcagcccct gggcggtaag gcccagttcg gcggtcagcg
     7801 gtttggcgag atggaggtat gggctctgga agcctatggt gccgctcata ctctgcaaga
     7861 gatattgacc gttaagtccg atgacgtggt gggcagggta aagacctacg aggccatagt
     7921 caagggcgag aatataccgg aacccggggt gccggaatcc ttcaaggtgc ttataaaaga
     7981 gctccagagc ctggccttgg atgtaaaggt gctctcggag gagagccggg agatagagat
     8041 tctggaagat tatagcgatg aatacgacga gctggacgtc aacatcgagg gacgcgagac
     8101 gacagaggaa gaagacgggg aagaggatct ggaggacttt gatttagatt ccattataga
     8161 cattccggac ctgagcgata ccctctttaa cgatctggat gagttgacgg atacagacga
     8221 cgacgatgac gatatagaat aggaagggag agagagtcct tgtttgaatt gaataacttt
     8281 gattcaatcc aaattggttt agcctcgccg gacaagatca gggaatggtc caggggtgag
     8341 gtaaaaaaac ctgagaccat taactacagg accttgaaac cagaaaagga tggacttttt
     8401 tgtgagaaga tatttggccc ccaaaaggat tgggaatgcc attgcggtaa atataaaagg
     8461 gtaagataca agggtataat ctgtgacaga tgcggtgtag aggtaacccg ttcaaaggtc
     8521 cgcagagaga ggatgggcca tatagaactg gctgcgtcgg tttcccatat ctggtacttt
     8581 aaggggattc ccagcaggat ggggcttatt ttagacatat ctccaaggtc tttggaaaag
     8641 gtcctgtatt ttgccgccta tatagtcata gatcccgggg aaacttctct cctaaagctc
     8701 cagcttttaa ccgataggga gtatcgcgag aattatgaca agtttggcaa cgaatttaag
     8761 gcgggaatgg gcgccggagc cataaaggag atcctcgagg gccttgatct tgataagatg
     8821 tcaagggagc tgaaggagga acttaagacc gccacaggcc aaaagcgcat aagggtggtt
     8881 aggcgactgg aagtagtaga agccttccga aaatccggta acaggccgga atggataatc
     8941 ttagacgtaa ttccggtaat cccgccggaa ttaaggccta tggtgcagct ggacggcgga
     9001 cggtttgcta cctccgacct caatgaccta tacagaaggg ttataaacag aaacaaccgc
     9061 ctaaagaggc tgttggatct ggctgctccg gatatcattg tcagaaacga gaaacggatg
     9121 cttcaagagg ccgttgacgc ccttatagat aatggcagga ggggcaggcc cgtaacaggt
     9181 cccggaaaca gacccctaaa atccctcagc gatatgctaa agggtaagca gggtaggttt
     9241 aggcaaaacc ttctagggaa aagggtcgac tattcaggac gctccgtaat cgtggtgggc
     9301 ccagaactaa agatgtacca atgcggactc cccaaggaga tggctctgga actctttaag
     9361 ccctttgtga tgaagagact ggtagaaaag gggcttgccc ataatataaa gagcgccaag
     9421 aggatggtag agaaggtaaa ggccgaggta tgggatgtac ttgaggaagc aattacggaa
     9481 catccagtcc ttttaaaccg cgctcctaca ctccacaggc tgggaataca agcctttgag
     9541 cctgtattgg tggaaggccg ggcaataaag ctccatccat tggtatgtag cgcctacaat
     9601 gcggactttg acggtgatca gatggccgtc catgtgcccc tgtccgtgga agcacaatcg
     9661 gaggcaaggt ttttgatgct gtctaccaac aacatcctaa agccttcaga cggccgaccc
     9721 gtagtgagtc ctacccagga tatggttata gggatatact atttaaccag cctgaaggaa
     9781 gattcgaagg gcaagggtaa gttatttatt tcccccgacg aggttataat ggcctatgga
     9841 gccggggtca ttgatcttca cgcccccatc aagatgaggg tggaaaggga gatcgacggg
     9901 aaaaaagagg cgaggctaat agacgtaacg gcgggaaggg ttatctttaa tgaggctatc
     9961 ccccaggatt tggggttcag ggacagaacg aaccccgatg agttatttga gttggaagta
    10021 aacgaagtgg tggacaaaaa ggttatcggt aggattgtag acaggtgtta cagaaagtat
    10081 ggaacttccg gaaccagcat cgtactggat aagctcaagg acttagggtt ccactattct
    10141 accataggtg ccgtcaccgt cagcgtttcg gacatagtga ttcccgaaga aaagcagggc
    10201 cttttagaga aagcagacca attggtcctg ggcgtagaca gacaatacag aaggggtctt
    10261 atctcggata ccgagcgtta tgaaagggtt ataaatacct ggaacaagac catagatgat
    10321 gtgacagatg ccctaatggg cagtttagat cactttaacc ccatatatat gatggcaaat
    10381 tccggtgcca ggggcagcat caaccagata cgacagttgg cgggaatgcg tgggcttatg
    10441 gccaacccca ccggaaagat catcgagctt cccatcaggg cgaactttcg cgaggggctt
    10501 acggtgctgg agttctttat ttccacccac ggcgcacgta aaggtctggc ggatacggcc
    10561 ctcaggactg cggattccgg ctaccttacc cgaaggctgg ttgatgtcag ccaggatgta
    10621 atagtaaggg aaggggattg cttcgcaaag acagggcaaa aaccccaggg aatcttggta
    10681 gacgagataa aggatgccga tgaagttatc gagccattca tagacagaat aacgggcaga
    10741 tatgtggccg aggatataaa tcaccccgag accggggaaa ttatagccat ggccaacgag
    10801 aggatcgacc atgacttagc cgaggagata gtcagggcgg gtatcactaa ggcgaatatt
    10861 cgttccgttc ttacttgcag gacggagcac ggcgtatgcg ccaagtgcta cggcagcaat
    10921 atggcttccg gcgaaccggt gaacatcggc gaagccgtgg gtattatcgc agcccaatcc
    10981 atcggcgaac ccgggaccca gctcaccatg agaaccttcc atacaggcgg aatagccggg
    11041 gcagatatta cccagggtct tcccagggta gaggagctgt ttgaggcccg aaagcctaag
    11101 ggattggcgg ttatttcgga aatatccggt atcgttaaga tcaacgaaac caagaaaaaa
    11161 agggaagtgt ccatcatggg cgcggatggg gataccaaga actataccat accctatggt
    11221 tcccgtataa gcgtggtaga ggaccagcag gttgaggccg gagatgagct tacggaagga
    11281 tccataaacc cccatgacat cctaaagatc agaggggtca agggagttca aagctatctc
    11341 ctccatgagg ttcaaaaggt ttacaggatg cagggcgttg atataaacga taagcatatt
    11401 gaagttatcg taaggcaaat gcttaaaaag gtaagaatcg aggatgcggg ggacaccgat
    11461 atgttgccgg gtggactcat agacattttc gagtttgaaa gagagaacga caaggtagta
    11521 gaggaaggcg gccaaccggc cttcgccaag agagtgctcc tgggtataac caaggcctcc
    11581 cttgccacgg aatccttcct gtctgcggcc tcgttccagg agacgaccag ggttcttacc
    11641 gaggcttcca taaagggtaa atccgatccc ttggtaggcc ttaaggaaaa cgtgattata
    11701 gggaagctga tccccgcagg gacgggcatg agcagataca aagatataga ggtttatacc
    11761 gaagaagaac ccgaggaaat aattcccgtg gatatagaag aggaagaacc ggttttacag
    11821 gcttaggaag ttggaggttg gaggttagaa gctagaagcc cgatctcatg gggctgttgc
    11881 tagggaggat ggatttgtgg aggcggattc caacagcccg aatggaagac tcctaagggg
    11941 ttagaaattg gaggttgaac cctacagtga tgttactaca ggagtaatga actattgaaa
    12001 agtggatgac ttcattgatt tggcaaagaa aacttggctt atggaatgca gaaaagccgt
    12061 agtttaaggc aggacgcctt aaaccgattc caccggcagg acgccggatt ggaagcgata
    12121 ggcttttcaa atggaataag ccttagtttt ctccaaatct atgttggaag gagcgttcaa
    12181 tagtcattac gtggtattgc gttcaccagt cattacgtaa gcactgggtt caataaccat
    12241 tacggaagta ccggatttga cagcgttact tacatgtgcc gacagatgcg gcgccttaat
    12301 taaaacccct aacctccccg gccggctcgg ctttattccg gcaatgcttt aaataataat
    12361 tattgacatt ggatttgtac aatgatagaa tgtttgagtg cgcaatttga cgggcaccct
    12421 gaattagata tttaggagtt gaaaaacatg ttagagggcc tgagggaggc ttcaaacaag
    12481 actgtgggta tgaagcaaac ccttaaagca gttcaagggg gattggcttt aaaagtcttt
    12541 ctagccgggg atatcgacga ctatatatcc cagaagataa ggcatgagtg tcaaaggcat
    12601 gatatcgaca tcattatggt agattccatg aaggagctgg gtgaagcctg cggtataagt
    12661 gttggcgctg caacagccgc catccttgaa gttttagatt agagcagctt taaacctatt
    12721 attttgaaag gaggtggcat aatgcctacg attagtcagt taataagaaa aggcagaaaa
    12781 agcatcggac acaaatccga cgcacccgca cttaagggta gtccccaaaa gaggggggtt
    12841 tgtaccgctg tcagtacccg tacacctaaa aagcccaact ctgctcttag aaagcttgca
    12901 agggtaaggc tgtccaacgg aatggaagtc acagcctata taccgggaat cggacataat
    12961 ctacaggagc actctgtggt gcttattcgc ggagggaggg ttccggacct acccggggta
    13021 cgttatcata tagtacgcgg cgctttagat acggccggag ttaccgaccg tcagcaagcc
    13081 agatccaaat atggtgccaa gaagcccaga aagtaatcat tttaagtagt cctgtacaga
    13141 aggagggaag tatatgccaa gaaaaggtca tgtgcccaaa agagatgtgt tgcccgatcc
    13201 attatatggc agtaaagttg taaccaagct gataaaccag gttatgctgg acggtaaaag
    13261 agggatagct caacgaattt gttacggggc cttcgatatt atcagagaca aaacaggtaa
    13321 cgagcctatt gaggtttttg aacaggctat gaacaatata atgccggtgc ttgaggtaaa
    13381 agcccgcagg gtgggtggtg ctacctatca ggtgcccatg gaagttagaa cagaaagaag
    13441 acaaaccttg ggccttcgct ggctggtaag ttttgcccgc aaccgcggag agagaaccat
    13501 gatagataga ctggcatctg agattatgga tgcagctaat aacatgggtg gtgcagtgag
    13561 aaggaaagaa gatacccata agatggcaga agctaacaga gcctttgctc attatcgttg
    13621 gtaacctata aagccactgg ccaattatta acctgggact aaaaagtccc attattttag
    13681 tgaatcgtca actgaaagga ggcaaagaga tgcctaggca attttcgctg gaaaacacca
    13741 gaaacatagg aattatggca cacattgatg ctggtaagac aacaacgtca gagcggattt
    13801 tgttctacac cggaagggtt cataagatgg gcgaaaccca tgagggttcg gctactatgg
    13861 actggatggc ccaagagcag gaacggggta taaccatcac ctcagccgct acaaccgcac
    13921 aatggaaggg ataccgtatc aacattatag atacgcccgg gcatgtggac tttaccgtgg
    13981 aagttgaacg gtcgctgcgc gtattggacg gctcggtggc aatattttgt gcaaaaggcg
    14041 gtgtagagcc ccaatcggag accgtatggc gtcaagcgga taagtatcga gtgcccagaa
    14101 ttgcttatat aaataagatg gacataatag gtgcagatta ttttaatgta gtcgaaatga
    14161 tgaaggaacg ccttggggca aacgctgttc cattacagat ccccatcggc aaggaagact
    14221 attttaaggg cataatcgat cttgtagaga gaagggctat ttactacacg gacgacttag
    14281 gcacccatag cgaagaaacc gatataccgg aggatatggc ggacctggta caagaataca
    14341 gggatatctt aattgaggcc gtagccgaaa acgatgagga tctcatgatg aaataccttg
    14401 agggtgagga attgacccct gatgagatca aggcggggat cagaaaggca acggtttccg
    14461 tagatatgat tccggtattg tgcggttcgt cctataagaa taagggcgta cagccgctat
    14521 tggatgcggt tatagagtat atgccgtcac ctttggaggt ggcagcaatt aaaggtacga
    14581 taccggggac ggacgaggaa acagaaaggc attcctccga cgaagagccc tttagcgcgt
    14641 tggcctttaa gatcatgacg gatccctttg tcgggaagct cgcttttttc cgggtatact
    14701 ccggtacatt aaaatcgggc tcttatgttt ataactccac aaaggggaag cgagagagaa
    14761 tcggaagaat cctacttatg cacgcgaacc atcgcgagga agtcgatgaa gtatattcgg
    14821 gagatattgc ggccgccata ggccttaggg atacaacgac aggagatacc ctatgccttg
    14881 aggtcgaacc ggttatttta gagtccatgg aattccccga ccccgtaatc caagtggcta
    14941 tagagcctaa aaccaaggtc ggccaggata agatgggggt agcgcttcaa aagctggcag
    15001 aggaagaccc aacgtttagg acctatacag acggggaaac agggcagact atcatcgccg
    15061 gcatgggtga gcttcatctg gagatcatag tagacaggtt gctcagagaa tttaaggtag
    15121 aggccaatgt gggaaaaccc caggttgcct acagagaaac aatacgcaag gcggtcaagt
    15181 ccgaaggacg atttgttcgt cagtcaggag gaaggggtca atacggccat gtatggctgg
    15241 aggtagaacc cttagaggcc ggaagcggtt acgagtttgt aaacaagatc gtcggcggag
    15301 tggtcccgag agaatatgtt gcgcctgtag atcaggggat acaagatgcc atggccagcg
    15361 gcattttggg cggatatccc gtagtggata ttaaggcaac attgtatgac ggatcctacc
    15421 atgaggtgga ctcatcggaa atggcgttta aaatagcggg ttctatagcg tttaaggatg
    15481 ctatgagaaa ggcaaacccg gtccttatgg agcccaccat gaaggtagag gttacggtgc
    15541 ccgaagaata tatgggcgac gtaatggggg atattaattc caggcgcggc agaatcgaag
    15601 gcatggaagc caaatccgga gtacaggtga taacatcata tgtaccctta tcggagatgt
    15661 ttggctatgc tacagacctt cggtccaaga gccagggaag aggcgtttac accatgcatc
    15721 tatcccatta tgaggaagtc ccaaagagta tagccgaaaa gattgtagct aaatagagat
    15781 cctaagagca cattaattaa ggaggaagaa ggaaaatggc gaaagcaaaa tttgaaagaa
    15841 caaagcccca tgtaaatata ggaaccatag ggcacgttga ccatggcaag accaccttga
    15901 ctgcggccat taccatggta ttgtcc
//
LOCUS       DASRNX010000081        17190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_174278,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000081 DASRNX010000000
VERSION     DASRNX010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17190
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174278"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VFD89_03265"
     CDS             complement(<1..814)
                     /locus_tag="VFD89_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZJ57241.1"
                     /translation="MDNYYSRLTDFYGNTSPRELIHKYGSPLYVYNESILRQRCREMA
                     SLVSYPRFTVNYSCKANTNLELLKIIHQEGIHADAISPGEVHVLQKAGYRPEEIFYIS
                     NNISREEMRTVTDMGIIISIDSLSQLEQLGEVNPGGNVAIRLNPGLGVGHHKKVITAG
                     DDTKFGIDLELIPEVKDIANRYDLKIVGLNQHLGSLFMKGDIFVEGAKLLLAAAKGFH
                     DLDFIDIGGGFGIPYRKQDNEPRLELNEFGDNLSAVLSDWAKEYGKQVEFIIE"
     gene            1281..2810
                     /locus_tag="VFD89_03270"
     CDS             1281..2810
                     /locus_tag="VFD89_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HZJ57242.1"
                     /translation="MLAKVKSAGIFGVEGYMIDVEVDISNGLPVFDIVGLPDLSIRES
                     KERVRAAIKNSGFEFPIKRITVNMAPADTKKEGPAFDLAIAIGILTATGQIPVMTKPD
                     PVFLGELSLDGTLKPISGVLPMLLSIREENEAVVLPYENAEEASNVSEVAVYPYKTLA
                     ELVECFRGKAGLTEYQKTCSHDAGFKPSFTDDFFDVKGQDAAKRALEVAAAGGHNIIL
                     MGPPGSGKTMLAKRLPTILPDLAYNEALEITKVYSVAGMLKEKEGIIGSRPFMSPHHT
                     ISSSALVGGGRIPAPGQISLAHHGVLFLDELLEFKKDVLEVLRQPLEDGEITISRAQG
                     SALFPCKFMLVASLNPCPCGFLGDSGHECTCTPNQIRRYLARISGPLLDRFDIHIEVP
                     SLSFQKISGDEKAESSSDIRERVNRAREIQVNRYKKDGIYYNSQLKPRQIRTFCKTDE
                     LGNTLLERAFVSMRLSARAYDRILKVSRTIADLEGSDNVEAGHIAEAIQYRSLDRQYW
                     R"
     gene            2869..3969
                     /gene="dprA"
                     /locus_tag="VFD89_03275"
     CDS             2869..3969
                     /gene="dprA"
                     /locus_tag="VFD89_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZJ57243.1"
                     /translation="MKQIDERIYWIWLSQIPGVGAKRFHFLVEHFSGAHGVWAAPKGE
                     LLKLIPHLGPSVVNEIANQRQNRILDKIYESSNSSDYRIVTLLDGEYPGLLKEIFDPP
                     PILYCKGLPIDDNNPALAIVGSRRSTGYGRQMSERFGFQLAEAGVTVVSGLARGIDAM
                     SHYGALKAGGKTIGVLGCGIDRVYPPENRKLFQTMEEKGTIITEYPPGTSPLPGNFPA
                     RNRVISGMADGVLVIEAGLRSGALITVDFALEQGRDVYALPGNINVPQTVGTNKILKE
                     GAKLITDIRDILEDLGISLADPGEAPGSVQLDFFESQVYNALQEGERHLEELARDTKM
                     EIHKLNAVLTMLEVKGIVKQMPGKIFIKSWDA"
     gene            3989..6076
                     /gene="topA"
                     /locus_tag="VFD89_03280"
     CDS             3989..6076
                     /gene="topA"
                     /locus_tag="VFD89_03280"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZJ57244.1"
                     /translation="MAQNLVIVESPAKAKTIKKFLGRNYKVEASMGHVRDLPKSQLGV
                     DVENDFEPKYITIRGKGEILSKLRKEAKTAKKILLATDPDREGEAISWHLAHILKIEA
                     DSPCRIEFNEITGTAVKEAVKKPRPIDKDLVDAQQARRILDRIVGYQISPLLWRKIRK
                     GLSAGRVQSVATRIICDREREIQGFVPEEYWSLIGTFYKDGDKQVFEANFYGKNGKKL
                     ALNEQKQVDKIMQDLKGADFAVQEVKRGNKRRNPSPPFTTSTLQQEASRKLRFTAQKT
                     MLLAQQLYEGIDIKGEGSVGVVTYIRTDSTRISEGAQTEALEYILSKYGKKFRPDKPN
                     QYRGRKGAQDAHEAIRPTSILRDPDSIKDSLKRDQYRLYKLIYQRFLASQMTPAIFET
                     MTINIKGRDYIFRANGSRKVFQGYSIIYEEGRDDGKEEKDIDLPDLKAGEKVILEKWT
                     PNQHFTQPPPRFTEASLVRALEDMGIGRPSTYAPTIYTITSRGYVEREKRTLIPTELG
                     FIVNDLMTDYFADIVDYQFTADMEERLDGVEEGERVWKDVIRDFYPSFKSLLDHADGD
                     IPKIEIKDEESDVICEKCGANMVIKTGRYGKFLACPNFPECRNTKPIVEEIDVPCPKC
                     GSNVVVRKTKKGRKFYGCSKYPECDFVSWDLPVKEPCPECGSYMVEKTQRGGTKTVIC
                     SNDKCKYKEEKSK"
     gene            6076..7383
                     /gene="trmFO"
                     /locus_tag="VFD89_03285"
     CDS             6076..7383
                     /gene="trmFO"
                     /locus_tag="VFD89_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HZJ57245.1"
                     /translation="MELKVTIIGGGLAGCEAAWQLASMGIKVELYEMRPQVMTPAHTT
                     SNLAELVCSNSLRSDRLENAVGLLKEEMRRLDSLIIKAADLNRVPAGGALAVDRDSFS
                     GYITDAIKGHPNIVVHYQEVELIPESGYIIIATGPLSSANMAGAISQLIKEDYLYFFD
                     AAAPIVTLDSLDESKVFRASRYGRGDDYLNCPMDREEYTKFWNELINAEVVELKDFEK
                     RIFEGCMPVEVMGKRGFDTLRFGPLKPVGLKDPRTGKEPYAVVQLRQDNHKASLYNLV
                     GFQTNLKFPEQRRVFSLIPGLENAEFVRYGVMHRNTFINSPKLLDSFYRLREDHRIYF
                     AGQITGVEGYVESASSGLIAGYHLARTLLGKQVAAFSGTTAIGALSRYISNPAIGDFQ
                     PMNINFGIIDPLGQRIRNKQQKNKSIAQRALKAIEEYINTHTT"
     gene            7484..8014
                     /gene="hslV"
                     /locus_tag="VFD89_03290"
     CDS             7484..8014
                     /gene="hslV"
                     /locus_tag="VFD89_03290"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HZJ57246.1"
                     /translation="MLKGTTIVAVRKDDRAAIGGDGQVTFGQNTIMKHGARKVRRLYH
                     DRVLVGFAGSVADAFSLSERFEAKLEQYSGNLERAAVELAKEWRADKILRKLEAMLIA
                     IDDRTLLIISGTGEVIEPDDQVAAIGSGGSFAMAAAKALMENTKLEPKEIVRKSLEIA
                     SSICVYTNDNIYIEEI"
     gene            8027..9424
                     /gene="hslU"
                     /locus_tag="VFD89_03295"
     CDS             8027..9424
                     /gene="hslU"
                     /locus_tag="VFD89_03295"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HZJ57247.1"
                     /translation="MDNLTPKQIVSQLDRYIIGQNEAKKSVAVALRNRFRRSLIEEDL
                     REDITPKNIIMMGPTGVGKTEIARRLAKLVEAPLVKVEATKFTEVGYVGRDVESMIRD
                     LVEAAIRMTKNSKTESIKEKAELAAENRILDILLPTKKRKQPNPLGLLFQQDESHEDS
                     DEAPVDEQSTQSMAGTREKFRQRLRLGTMEDMQVEIEVEDSKYSGIGFIPGMGNEDML
                     IQIQDVFGSIMPRKTKKRKTTIREARRIFEQEEAQKLVDMDEVIEEAVEAAEQHGIIF
                     IDEIDKIAGKEMSSGPDVSREGVQRDILPIVEGTTVMTKYGPIKTDHILFIAAGAFHV
                     SKVSDLIPELQGRFPIRVELGSLTQSNFEQILTQPENALLKQQKELMKVENINLSFSH
                     EAIAEIARISFILNQSSENIGARRLHTVMEKLMEDISFNAEDLSGQDIRIDADYVHKA
                     LKDLVHEENLHKFIL"
     gene            9614..10393
                     /gene="codY"
                     /locus_tag="VFD89_03300"
     CDS             9614..10393
                     /gene="codY"
                     /locus_tag="VFD89_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HZJ57248.1"
                     /translation="MSESLLSKTRRLNKIIQRSGSDPVRFSDLARVLSEILHSNIFIA
                     SRRGRILGYALADEECFKSWGDDISEMSSFTQQCNSQFLKFNETLANINLEKGCHIVP
                     YAPGDDPYKFTSIVPISCGGDRLGTLIIERMAEEYCDDDLVLAEYSATIIGMEIINAK
                     AVEIEKEAREKTVVQMAVRTLSYSELEAIEHILNELNGNEGLLVASKIADRVGITRSV
                     IVNALRKLESASVIESRSLGMKGTHIRVLNDKLMDEIERNR"
     gene            10416..10850
                     /locus_tag="VFD89_03305"
     CDS             10416..10850
                     /locus_tag="VFD89_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZJ57249.1"
                     /translation="MHDGMIALKMALRKNGYKLTSQRKATLETILENQGKHLSTEEIF
                     VEVKKTNPRIGLATVYRTLLLLEELGILIKHNFEDGRNRYELGHPEQDHDHHHLICLK
                     CGEVSEVKEDLLDALEEIIDKNYGFKVANHKVKFYGYCKECT"
     gene            11094..13523
                     /locus_tag="VFD89_03310"
     CDS             11094..13523
                     /locus_tag="VFD89_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZJ57250.1"
                     /translation="MKGIIMAGGAGSRLRPLTCDLPKPMVPVMNRPVMQYSIELFRKY
                     GINDIGVTLQYLPEAIQDYFGDGGRDPINLHYFIEEKPLGTAGSVKNAQEILDDTFIV
                     LSGDALTNIDLEKAADFHRQNQSMATLVLKKVEIPLEYGVVMTHGDGRIARFLEKPGW
                     GEVFSDTVNTGIYILEPQVLEYFETGVKFDFSKDLFPRLLEDRKPMFGYITEEYWCDV
                     GDTNSYLQSHFDIMNGDAGIKPDAKELEDGVWVEEGVYIHPGASIAAPCYIGRNTTIG
                     EGVSLHPFTVIGENNHIGEHSSIKRSVLWNHNSLGRNVEARGAIVCDNTRVGNNCSIF
                     EGSVVGKNTHLHERVVLKPDAKIWPCKTIEQGMTIDENIIWGTRLKRTLFGKDGIRGC
                     FNIEIDAGFVNRLGTAYGTELKEDSRICISSNGHEAAIMLKHSLMAGVLTSGLSIFDI
                     GSTITPVTRYAVRHLGLDGGIHIRCEDDDPNRVHIGLIDNNGANLPDSMERKIENIFI
                     SGDFSVKSPNNIKKVSIINDIEMFYIRHLINSIDAEAIKQRKYRILLSAKSKSLASLF
                     MAMTRDLGCRVEVLKGQIMDAESIKGYDLAIGFDDDGERLEILDEGGEAIKEGTMVAL
                     ISLVCFKQDKDVGVVIPNTAPSAIEKMAKNYNGSVIRSKTKRRAMMEEVIKAGDSEKR
                     KVMERFILYFDGLATVMGIMDLMAREDKKLSDLVEDLPGIYMAIRDIECPWTAKGKVM
                     RSLIDEANRGDSDVELYEGVKITEDKGWTLILPDSDEPLVKIYSEGFSEEYAEELSDF
                     YEKRINGIKEQ"
     gene            13757..15424
                     /locus_tag="VFD89_03315"
     CDS             13757..15424
                     /locus_tag="VFD89_03315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZJ57251.1"
                     /translation="MTSKNNNTLKLIPLGGVNEIGKNMTLLEYGDEIIVIDCGLSFPE
                     EEMLGIDLVIPDISYLVKNKEKVKAIVVTHGHEDHIGALPYVLKEINVPVYGTRLTLG
                     LIENKFKEHSLDKVKLKCVKAGDRVRLGNFMVDIIRTSHSIADAVALAVHTPVGVIVH
                     TGDFKIDYTPIDGQVVDFAKFAKLGEKGVLALLADSTNVERPGYTLSERVVGATFQNI
                     FGTAKGRIIVATFASNIHRIQQIIDAAVKYNRKICVSGRSMVNVMSVAMELGYIKVPG
                     RTLIEIDEIQNYPDEKIVIITTGSQGEPMSALSRMASSDHRKIEIIPGDMVIVSANPI
                     PGNEKLISKVINQLFLRGADVIYEALADIHVSGHACQEELKLIHTLVKPKFFIPVHGE
                     YRHLKQHANLAESLGMARENIKVLDIGNVVEFTPDSCKVTGSVSAGGILVDGLGIGDV
                     GNIVLRDRRHLSQDGLMVIVVTISKVSGLSVAGPDIISRGFVYVRESEQLMEDAKEVI
                     RGILEDCEENQITDWGTMKYKIKAGLRSFLYEKTKRNPMILPIIMEI"
     gene            15783..16919
                     /gene="mltG"
                     /locus_tag="VFD89_03320"
     CDS             15783..16919
                     /gene="mltG"
                     /locus_tag="VFD89_03320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZJ57252.1"
                     /translation="MSQKGTVFLKRILRQVLIFLLSILIVASTVYFGINTAYSRYIKP
                     VDAGNSSLVDIEIPRGSSLSKISEILYENNLIRNKMVFKLYVDISNKTSRLKAGKYKL
                     SRDMDLDRIMDELLTGNAAIDTIKITIIEGWDIRRIARYLIEEKEYGFSEKEFIDAAK
                     IENFTDFVFLQDIPEERKTGEVGIAPLEGYLFPDTYLVYEDASPEDIMRKMLTQFEKV
                     YNESVMERAQELEMDMDEVVTLASVIQREARITEEFPMISAVFHNRLKKDMPLGADST
                     IQFLVNEDRWAFSSEELKIDSPYNTYENQGLPIGPISSPGRLALTSALFPYEEFMNSK
                     KPYLYFVLKDPKTGEHVFNTDYNQHMKDKKEYEASWKDIGNQED"
     gene            complement(17011..>17190)
                     /locus_tag="VFD89_03325"
     CDS             complement(17011..>17190)
                     /locus_tag="VFD89_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57253.1"
                     /translation="ASAPYAKATLSFSRLPAGANSSGVLKDITFSPYNNKTSRQDVSR
                     LYKRRVHISKNRRWV"
BASE COUNT         5223 a         3265 c         4407 g         4295 t
ORIGIN      
        1 gctcgattat aaattctacc tgtttgccgt attccttcgc ccaatcgctt agaaccgcgg
       61 aaagattgtc cccaaattca tttaattcaa gtcgcggttc attgtcctgc tttcggtagg
      121 gtatgccaaa tccgccgcct atgtctataa aatccaaatc gtggaatcct ttggcggcag
      181 ccagcaatag ttttgcacct tcaacaaaga tatccccctt cataaacagg gatcccaaat
      241 gctgattaag acccacaatt tttaggtcat atctgttggc tatatccttt acttcgggga
      301 ttagttccag gtctatacca aacttggtat catcgccggc agttattacc ttcttgtgat
      361 ggcctacgcc gaggccgggg tttaaccgaa tggctacatt gccgcccgga ttcacttcgc
      421 cgagctgttc caattgagat aaggaatcta tgctgataat aatgcccatg tctgtaaccg
      481 tcctcatctc ttctctggaa atgttgttgc ttatataaaa gatttcttca ggtctatacc
      541 ccgccttttg taatacatga acctcgccgg gagaaatggc gtctgcatgg attccctcct
      601 ggtgtatgat cttaaggagc tctaaattgg tgttggcctt gcaggagtaa ttgacagtaa
      661 agcggggata ggaaaccaat gaggccattt ccctgcatct ttgtctcagg attgactcgt
      721 tatacacata cagggggctt ccatatttat gaataagctc cctaggcgat gtgttgccgt
      781 agaaatcagt taatctggaa taataattgt ccatagtata tgcctcctat ctagcaatgg
      841 ggatagggtt gataccccat aataaccaaa ataatttcgt catattcgta gtaaagtcta
      901 ggatgaaact tgggattgat gttcgattat gccggatttt aggtagaggc cccagtccgc
      961 accgctatat ggagcgggag gggaaaccga ccttaggagc agctaatccg tggcaccatt
     1021 gtagtacatg aatcatgcga atgcttttcg atatttcgga cctgatgaat atgtttatcg
     1081 ccaagcaaac tgccagctat ataatagtag tcttaactat ccacataata cgtaaaaatg
     1141 cttttcttgt caagaaagac aggctttttc atcggagagc aggaaatccg gaataattgt
     1201 agaatatgtg ataatgtagg tgttagccgg gctttgatca caggttttag caagaaatta
     1261 agccttaagg aggcgacgga atgctggcta aggtaaaaag tgccgggata ttcggtgtag
     1321 agggatacat gatcgatgta gaagtggata tatccaatgg attgcccgta ttcgatatag
     1381 ttggtttgcc ggacttatcc atccgcgaat caaaggagag ggtgcgtgct gccattaaaa
     1441 atagtgggtt tgagtttccc atcaaaagga tcacagtaaa tatggcccct gcagacacca
     1501 agaaggaagg gcctgcattt gacttagcca tagcaattgg aatccttaca gcaaccggcc
     1561 agatccccgt tatgactaag cccgatccag tattcttggg tgaattgtcc cttgatggca
     1621 cacttaagcc cataagcggc gtgcttccca tgcttttgtc cattagggaa gagaacgaag
     1681 ccgttgtact gccctatgaa aatgccgagg aagccagtaa tgtatctgaa gtagcagtat
     1741 atccctacaa gacattggcc gaattagtgg aatgctttag gggaaaggct ggtcttacgg
     1801 aataccaaaa gacatgttcc cacgatgccg gatttaagcc atcatttaca gatgattttt
     1861 tcgatgtcaa gggccaagat gccgcaaaga gggctttgga agtcgccgcc gcggggggcc
     1921 acaatattat tcttatggga ccaccgggtt ccggtaaaac catgttagcg aagcgattgc
     1981 ccaccatcct tccagatcta gcctataacg aggctcttga gataacaaag gtctatagtg
     2041 tagcgggcat gttaaaggaa aaggaaggga ttataggttc tcgccctttt atgtccccac
     2101 atcatacgat ttcgagttct gcccttgtag gcggaggcag aatacccgcg ccgggacaga
     2161 ttagcctagc ccaccatggt gtcctattct tggatgaact tcttgaattt aaaaaggatg
     2221 tcttggaagt tttgagacaa cccctggaag atggggagat tacaatttcc agggctcagg
     2281 gttccgccct tttcccctgt aaatttatgt tggtagccag ccttaatcct tgtccatgcg
     2341 gatttctagg tgactctggc cacgagtgca cctgtacgcc caatcagatt cgcagatacc
     2401 ttgccaggat atccgggccg ctccttgaca gatttgacat acatattgag gtgcccagtt
     2461 tatcctttca aaagataagt ggagatgaaa aagccgaatc atcatctgat attcgcgaga
     2521 gggttaaccg agcgagggaa atccaggtca accgctataa aaaagacggg atatactaca
     2581 attcacaatt aaagcctagg cagatcagaa cattttgcaa gacggacgaa ttgggaaata
     2641 cccttctgga aagggctttt gtctcaatga gattgagtgc cagggcgtat gatagaattc
     2701 ttaaagtttc aaggactata gcagaccttg aaggatcgga caatgtagag gccggccata
     2761 tagcagaggc tatacaatac aggtcattgg acaggcagta ttggcgctaa gcgctatggt
     2821 taattattgc taataatata cgtatgcttt aaagcagggg ggatatactt gaaacaaatt
     2881 gacgaaagaa tatattggat atggttatcg cagatccccg gtgtcggggc taaacggttt
     2941 cattttctgg tggaacactt ttccggggcc catggggtat gggctgcacc aaaaggggag
     3001 ctgttaaagc taatccccca tctaggccct agtgttgtca atgaaattgc aaatcaaaga
     3061 cagaacagga tcttggataa gatctatgag agttccaatt cttcggatta taggattgtc
     3121 accctgctgg atggggaata tcccggttta ttaaaggaaa tatttgatcc accccctatt
     3181 ctctactgca aaggcttgcc aattgatgac aataaccctg cgttggccat cgtaggttcc
     3241 agaaggtcca cgggctatgg aaggcaaatg tcagaaagat tcggattcca gttagctgag
     3301 gcaggtgtta ccgtagtgag cggcctagcc agaggcatag atgccatgtc ccattacggg
     3361 gctttaaagg ctggcggaaa gaccataggg gtattaggtt gcggtataga cagggtttat
     3421 ccgccggaga accgtaaact atttcagacc atggaagaga aaggcacgat aataaccgaa
     3481 tacccgcccg ggaccagtcc attaccaggg aacttccctg ccagaaaccg tgttatcagc
     3541 ggaatggccg atggagtact ggtgattgag gcggggctga ggagcggtgc acttatcacc
     3601 gttgattttg ctttggagca gggtagggat gtttacgcct tgccgggaaa tataaacgtg
     3661 ccccaaacgg tgggtacgaa caagatccta aaggaaggcg caaaattgat aaccgacatt
     3721 cgggatatac tggaggattt gggaataagc cttgcagatc cgggggaagc gccgggatcc
     3781 gtccagcttg atttttttga atcccaggtg tataatgcac tccaagaagg ggagcggcat
     3841 ttggaggagt tagcaagaga cacaaaaatg gagatacaca aattgaatgc ggttttgaca
     3901 atgctcgagg ttaaggggat agtaaaacaa atgccaggca aaatatttat taaaagctgg
     3961 gatgcttaat accaatggag gttatagaat ggcacaaaat ctggtaatcg ttgaatcacc
     4021 agcaaaggcg aaaaccatca aaaagttttt agggaggaat tataaggtcg aggcatctat
     4081 ggggcatgtt agggacctgc ccaagagcca attgggcgtg gatgttgaaa acgactttga
     4141 accaaaatat atcacaataa ggggcaaagg ggaaatcctc agcaagttga ggaaggaggc
     4201 aaagacagcg aaaaaaatac tcctggccac agaccctgac agggaaggcg aggccatatc
     4261 atggcatctg gcccacatcc tgaagataga ggccgacagc ccctgcagga ttgaatttaa
     4321 tgagataacc ggtacggccg ttaaggaggc tgtcaaaaag cctagaccca tagataagga
     4381 tctggtggat gcccaacagg cgcgtaggat tttagatcgt atagtgggat atcagataag
     4441 tccgctttta tggagaaaga tacgtaaggg attaagtgca ggccgggtac aatccgtggc
     4501 aactaggata atctgtgata gggaaaggga gattcagggc tttgttcctg aagagtattg
     4561 gtcccttata ggcacctttt ataaagatgg ggataagcag gtgtttgagg ccaattttta
     4621 tgggaagaac ggcaaaaaac tggcgctcaa tgagcaaaaa caagttgata aaataatgca
     4681 ggacctaaaa ggcgccgatt ttgctgtcca agaggtcaaa aggggtaata aaagacgaaa
     4741 cccatcccca ccctttacta ccagtactct gcagcaggag gcatccagaa agttgaggtt
     4801 tacagctcaa aaaacaatgc tgttggccca acagctctat gagggtatcg atataaaggg
     4861 ggagggctct gtaggcgtcg tcacctatat aaggacggac tctaccagaa tatcggaagg
     4921 tgcccaaaca gaggcgctag aatatatact atcaaaatac gggaagaaat ttaggccgga
     4981 taaacccaac cagtacaggg gtagaaaagg tgcccaggat gctcacgaag caataaggcc
     5041 aactagcata ctgagggatc ctgattccat aaaggattct ttaaaaagag atcaatatcg
     5101 tctctacaaa ttaatctatc aaagattttt ggccagtcag atgacaccgg ctatctttga
     5161 aaccatgacc attaatatta agggaaggga ctatatcttt agggccaacg gatcacgcaa
     5221 ggtctttcag ggttattcca ttatctatga ggaaggccgt gacgatggta aggaggagaa
     5281 ggatatagat ttgcccgatc taaaggcggg ggaaaaggtg attctggaaa aatggacacc
     5341 aaatcaacat ttcacccagc cacctccccg ctttactgaa gcatcactgg tgcgggccct
     5401 agaggatatg ggaataggca ggcccagcac atatgcgccc accatatata caatcacatc
     5461 caggggatat gttgaaaggg aaaaaaggac gttgataccc accgaattgg ggtttatagt
     5521 caatgacttg atgacggatt atttcgctga tatagtggat taccaattta cggcggatat
     5581 ggaggagcgt cttgatggtg tcgaggaggg ggaaagggtt tggaaggatg taataaggga
     5641 tttttatcct tcctttaaat cactattgga tcatgcggat ggggatatcc ccaagataga
     5701 aataaaagat gaagaatccg acgtcatatg cgaaaaatgc ggtgcgaata tggtcataaa
     5761 gacaggacga tacggtaaat tcttggcatg tcccaacttc cccgaatgca ggaatacgaa
     5821 gcccatagtg gaagagattg atgttccctg tcctaagtgt ggttccaatg ttgtggtaag
     5881 gaagacaaaa aagggacgta aattttatgg atgcagcaaa tatcccgaat gtgactttgt
     5941 gtcctgggac ctgccggtaa aagagccgtg cccagaatgt ggctcctata tggtggaaaa
     6001 aacccaaagg ggagggacaa aaaccgtcat atgttctaat gataagtgta agtataaaga
     6061 ggaaaagagt aaatgatgga attaaaggtt acaattatag gcggcggttt ggccggatgt
     6121 gaagccgcat ggcaactggc cagtatgggc attaaggtgg agctttatga gatgaggcct
     6181 caagtgatga cccccgccca taccacctcg aatctcgcag aactggtctg cagtaattcc
     6241 cttagatccg ataggctgga aaatgccgtg ggattattaa aggaagagat gcgcaggctc
     6301 gattccctaa tcataaaggc tgcggatctt aaccgcgttc cggcaggcgg cgcattggct
     6361 gtggacaggg actccttttc aggatatatt accgatgcca taaagggaca tcccaatatc
     6421 gttgttcact atcaggaagt agagctgata ccggaatccg gatatataat tatagctaca
     6481 ggccccctgt catcggccaa catggcgggg gctatttcac agctgattaa agaggattac
     6541 ctatacttct ttgatgctgc ggcgcctatt gtaaccctgg attctttgga tgagtctaag
     6601 gtttttaggg cttctcgcta tggcaggggt gacgattacc ttaactgtcc tatggacaga
     6661 gaagagtata caaaattttg gaatgagctt ataaacgccg aagttgtaga attgaaggat
     6721 tttgaaaagc ggatatttga ggggtgtatg cccgtggaag tgatgggcaa aaggggtttc
     6781 gacacgctca ggttcgggcc cttaaagcct gttggtttga aggatccccg taccgggaag
     6841 gaaccctatg ccgtggtaca gttgaggcaa gacaatcata aggcttcatt atataacctg
     6901 gttggtttcc agacgaatct taagtttccc gagcaaaggc gggttttctc cttgatcccc
     6961 gggcttgaaa atgcagaatt cgttcgctat ggggttatgc atagaaatac ctttattaat
     7021 tcccctaagc tcctagactc cttttatcgg ttaagggagg atcataggat atactttgca
     7081 ggtcagataa ccggagtcga gggatatgtg gaatcggcat ccagcggcct tatagctgga
     7141 tatcacctgg caaggacctt gctcggcaaa caggttgccg cattttcggg tactactgct
     7201 atcggtgcct tatcccgata catttccaac cccgccattg gggacttcca gccaatgaac
     7261 atcaattttg ggatcattga ccccctgggc cagagaatcc ggaataaaca gcagaagaac
     7321 aaatctatag cccaaagggc cttaaaagca atagaggaat atattaatac ccatacgacc
     7381 tagtttccca tataagccct attaaaaagg ttgaattttg cgatattgta tgatactatt
     7441 tgaatgttta gtcaattaga gcattagggg ggtaagactg catatgttaa aagggacgac
     7501 catagtggct gtaaggaagg atgatagggc agcaattggc ggtgacggac aggttacctt
     7561 cggccaaaac accattatga aacatggagc cagaaaggta agaaggcttt atcatgatag
     7621 ggtattggtt ggatttgccg gtagcgtagc cgatgcattt agtcttagcg aacgcttcga
     7681 agcaaagctg gagcagtatt ccggaaattt agagcgagcc gccgtggagt tggcaaagga
     7741 atggcgcgcg gataagattt tacgaaaatt agaggcgatg cttatcgcca ttgatgatag
     7801 aacactcctg attatttcgg gaacaggtga ggttatcgaa cccgatgatc aggttgccgc
     7861 aattggatca gggggatcct ttgcaatggc agcggctaag gcacttatgg aaaacacgaa
     7921 gctggaaccc aaagaaattg taagaaaatc tctggaaatt gcctcttcca tatgcgttta
     7981 tactaacgac aacatctata ttgaagaaat ctaggaggtg gcgctattgg ataatttgac
     8041 cccgaagcag atagtttcac aattggatcg ctatattata ggacaaaatg aggcaaagaa
     8101 atcggtagca gtcgccctac gaaaccgttt taggcgcagc ctcatcgagg aggatttaag
     8161 agaggatatt accccgaaaa atataataat gatgggccct accggcgtag ggaagacgga
     8221 gattgcaagg cggctggcaa aattagttga ggctccccta gtcaaagtgg aggcaacgaa
     8281 atttaccgag gtaggctatg tgggtcggga tgtggaatcc atgataaggg atttggtgga
     8341 ggccgccatt cgcatgacca aaaacagcaa aacggaatcc attaaagaaa aggctgagct
     8401 ggctgctgaa aataggattt tagatatctt gctgcctaca aaaaagcgaa aacaaccaaa
     8461 tcctttaggg cttttgtttc aacaagacga aagccatgaa gactccgatg aagcccccgt
     8521 cgatgaacaa tccacccaat ccatggccgg aacccgggaa aagtttcgcc agagactaag
     8581 gttagggacc atggaggata tgcaggtgga gattgaagta gaggactcaa agtattccgg
     8641 gataggtttc attccgggca tgggaaatga ggacatgctc atacagattc aagatgtatt
     8701 tgggagcata atgccccgca agaccaagaa aagaaagact accatccggg aagccaggag
     8761 aatatttgaa caggaagagg cccaaaaatt ggtggacatg gacgaggtca tagaggaggc
     8821 tgtagaggcg gcagagcagc atggaattat atttatagat gaaatagata agattgctgg
     8881 aaaggaaatg agcagtggac ctgatgtttc cagagaaggg gtacaaaggg acattttacc
     8941 aatagtggag ggcactacgg ttatgactaa atacggtcca attaagacgg atcatatact
     9001 ctttattgcc gccggggctt tccacgtctc caaggtatct gatctaatac cggaactcca
     9061 aggcagattt cccataaggg tggaattagg gagcctaacc caatccaact ttgaacaaat
     9121 actgacccag ccggaaaatg ccctattaaa gcagcagaaa gagctgatga aggtggaaaa
     9181 cataaatctt tcattttctc atgaggccat tgctgagata gctaggattt cctttatttt
     9241 aaaccaaagc agcgagaaca taggcgcgcg aaggctgcat accgtaatgg aaaagctgat
     9301 ggaggatata tcgtttaatg ccgaggatct atcgggacag gacataagaa ttgatgctga
     9361 ttatgtgcat aaagccctca aggacctggt gcatgaggag aatctccata agtttattct
     9421 ttaagggggt ttcaaaaaaa cacataattc ataatttaaa gctggaattg tttatctacg
     9481 gctaagcaat acgaaaaata aatttatctg cagcaggcga actttatgac tagggttttt
     9541 atccacattt tacgtcacct aacccggcat ttatatatag ccatacaagg aatcaatcaa
     9601 ggagtgaatt agattgtcag aaagcttgtt gagcaaaacc agaagattaa ataagattat
     9661 acaaagatcg ggttcagatc ccgttcgctt ttccgatctc gcaagggtat tgagtgagat
     9721 tttacatagc aatatattta ttgccagccg caggggcaga atattgggat atgccctggc
     9781 ggatgaagaa tgcttcaaaa gctggggaga cgatatcagc gaaatgtcca gctttaccca
     9841 gcagtgtaac agtcaattcc ttaaattcaa tgagaccttg gccaatataa acctagaaaa
     9901 gggttgtcat atagtcccat atgctccggg agatgatccc tataagttta catccatcgt
     9961 tcccataagc tgtggggggg atcgccttgg aaccctgatc attgaaagaa tggcagaaga
    10021 atactgtgat gacgatctgg ttttggctga atacagtgct accattatag gaatggaaat
    10081 tataaatgca aaggccgtgg aaatagagaa agaagcacgg gaaaagaccg tggtgcagat
    10141 ggcggttaga accctgtcat attctgaatt ggaggcaatt gaacacatcc tcaatgagct
    10201 caatgggaac gagggtttat tggttgcaag taagattgca gaccgggtcg gcattactcg
    10261 ctccgtaata gtgaacgctc ttagaaaatt ggagagcgcc agcgttatag aatcccgttc
    10321 tctagggatg aagggtaccc atataagggt gctcaacgat aaattaatgg atgaaataga
    10381 aaggaaccga taaaacttaa aaaagaagtg attgtatgca tgatggaatg atcgcattga
    10441 agatggcatt aaggaaaaac ggttataagc ttacatcaca gaggaaggca acactggaaa
    10501 caatcttaga aaaccaaggt aagcacttga gcacggaaga aatatttgta gaggttaaaa
    10561 aaaccaaccc ccggattgga ttggcgaccg tatataggac tttgctttta ttggaggagc
    10621 tgggaatcct tataaagcac aacttcgagg acggtcgaaa tcgatatgag ctgggccatc
    10681 ccgaacaaga tcatgatcat catcatttga tttgtcttaa atgcggtgaa gttagcgagg
    10741 taaaagagga tctattggat gctttggaag aaattataga caagaattat ggttttaagg
    10801 ttgccaatca taaggttaag ttctatggct attgtaaaga atgtacttaa agccctgcgc
    10861 ttttaaaaat agcagggttt ttttatcccc atgtcatagc ggattgttcg gatattaccc
    10921 atggaaccat tgaccggctt atgtcccaag gagaagggaa cgtagtccgt acatccctgc
    10981 cccggggaca atgtgtttta ataacacccc aattggtata agttagtata aggatacaca
    11041 taataatttg gcaaagatca tattgcaaaa tactagcaaa agggaagata agaatgaaag
    11101 gtattattat ggcgggtggg gccgggtcca gacttcgtcc gcttacttgt gatttgccaa
    11161 aacctatggt gccggtaatg aacagaccgg ttatgcaata tagtatcgaa ctttttagaa
    11221 agtacgggat aaacgatatc ggtgtcaccc ttcaatatct gccggaagcg atacaggatt
    11281 attttgggga tgggggacgt gatcccataa acctccacta ttttatcgag gaaaagcccc
    11341 tggggacagc gggcagcgtc aagaatgcgc aggagatttt agacgatacc tttatagtcc
    11401 tgagcggcga cgcactaacc aatatcgatc ttgaaaaggc tgcagacttt catagacaaa
    11461 accaatccat ggccactcta gtcctcaaaa aagtagagat tccactagaa tacggggtag
    11521 tgatgaccca tggggatggg agaatagcca gatttttgga aaaacccggc tggggggagg
    11581 tctttagcga tacggtaaat accggtatat atatattgga accccaggtc ctggaatact
    11641 ttgaaacagg tgtaaagttt gatttcagca aggatctttt ccccagattg ctggaggata
    11701 gaaagcccat gtttggctac attaccgaag aatattggtg cgatgtagga gacaccaact
    11761 cctatctgca atcccatttc gacattatga acggggatgc ggggatcaag cccgatgcca
    11821 aagagctgga agatggtgta tgggtagagg agggggtcta tatacacccc ggcgccagca
    11881 ttgcggcccc ctgctatata ggtcggaaca cgacaatagg ggaaggggtc agtctgcacc
    11941 cctttactgt tatcggtgaa aataatcata tcggggaaca ctccagcatt aaaaggagcg
    12001 tattatggaa ccataatagc ttgggaagga atgtggaggc ccggggggct attgtctgtg
    12061 ataatacccg ggtgggtaac aattgcagca tctttgaggg aagcgttgtg gggaagaaca
    12121 cccatctaca tgagagggtc gtactaaaac cggatgcgaa gatatggccc tgtaagacca
    12181 tcgagcaggg catgactata gatgaaaata taatatgggg aacccgactc aagagaaccc
    12241 tttttgggaa ggacggtatc aggggctgtt tcaatattga aatagatgcc gggtttgtaa
    12301 atcgcttagg cacagcctat ggaacggaac tcaaagagga cagcaggatc tgcattagca
    12361 gcaacggaca tgaagcggct ataatgctaa agcatagtct aatggcaggt gtgcttacct
    12421 cggggctgag catcttcgat atagggagta caataacgcc tgtgaccagg tatgccgttc
    12481 gacatctggg gttggatggg gggattcata ttcgatgtga ggacgacgat ccaaataggg
    12541 tccatatagg tctgatagat aataacggag ccaatttacc ggattctatg gaacgaaaga
    12601 tcgagaatat atttataagc ggtgatttct ccgtaaagtc gccgaacaac atcaagaaag
    12661 ttagtatcat aaatgatata gagatgtttt atatcaggca tttgatcaat tccatcgatg
    12721 ctgaggcaat aaagcaaagg aaatatagaa tactattgag cgcaaaaagc aaatctttgg
    12781 cttcgctgtt tatggccatg acaagggact tgggctgtcg agtcgaagtg ctcaagggac
    12841 aaataatgga tgccgaatcg atcaaggggt atgatctggc cataggattc gatgatgatg
    12901 gggaaaggct ggagatcctt gacgaagggg gagaggccat taaagagggt accatggtgg
    12961 ctttaatcag ccttgtatgt tttaagcagg acaaggatgt gggagtggtg attcccaaca
    13021 cagctccaag tgctatcgaa aaaatggcaa agaactacaa tggcagcgta ataaggagta
    13081 aaaccaagag acgggctatg atggaagagg ttattaaggc cggagatagc gaaaagcgaa
    13141 aggtcatgga gcgttttatc ctgtacttcg acggattggc taccgtaatg ggaattatgg
    13201 atttaatggc cagggaagac aaaaagcttt cagatttggt ggaggatcta cccgggatct
    13261 atatggccat aagggatatc gaatgccctt ggacggcaaa gggtaaggta atgaggagcc
    13321 tgatagacga agcaaacagg ggagattctg atgtagagtt gtacgaaggg gtaaaaatta
    13381 ccgaagataa ggggtggacc ctaatcttgc cggattctga cgaacccttg gtcaagatct
    13441 atagcgaggg gttttcagaa gagtatgcag aggaattaag tgacttttat gagaagagaa
    13501 ttaacggtat aaaggaacaa tagggtccta ctgtaatcat gctaatcctt ttctgggatt
    13561 agcttttttt aagctattat cataaaaaag gatcattcgg gactccatag agccggaatt
    13621 ttcagcgtca taaccgtatt tgttgtagaa tgtgttttta tggtgatcat aacagttgca
    13681 ctgtttatat atatcaagta taatgggaac gttggatttg tgcggaaatc taataaaagg
    13741 aggaggaaat tttttagtga catcaaaaaa taataatacg ttaaagctaa ttcccttggg
    13801 gggtgtcaat gagatcggca aaaacatgac actcctagaa tacggggacg agatcatcgt
    13861 tatcgattgc ggtctcagct tcccggagga agaaatgctg ggaatagatt tggtgatccc
    13921 agacatatcc tatttagtga agaacaagga aaaggttaaa gccattgtag ttactcacgg
    13981 acacgaagat catatcgggg cgctgccgta tgtccttaag gaaatcaatg ttcctgtata
    14041 cggaaccaga ttgaccctgg gtttgattga aaataaattt aaagagcatt ccctggacaa
    14101 ggtaaaactc aagtgcgtaa aagcggggga tagggtaagg ctcggaaact ttatggtgga
    14161 tattatcagg accagccaca gcattgcaga tgccgttgcc ttagctgtgc atacacctgt
    14221 gggcgttata gtccatacag gggattttaa aatagattat acccctatag atggacaggt
    14281 cgtagacttt gccaagtttg caaaattggg cgaaaagggt gtgcttgcgc tgttggcgga
    14341 cagcaccaac gttgagcggc cgggatatac cctatccgaa agagtagtcg gagccacgtt
    14401 tcaaaacatc ttcggcaccg ccaaaggaag aataatagtg gctaccttcg cctcaaatat
    14461 acaccgaatt caacaaataa ttgatgcggc tgtaaagtat aacaggaaga tatgcgtttc
    14521 aggaagaagc atggtaaatg taatgtcagt cgctatggaa ttgggttata ttaaggtgcc
    14581 cggaaggaca cttattgaaa tagacgagat tcaaaactat cccgacgaaa aaattgtaat
    14641 aataactacc ggcagccagg gagagcctat gtccgcattg tcccgtatgg cctcgtcgga
    14701 tcacagaaaa atagaaatta tacctgggga tatggtaatt gtttccgcaa atccaatacc
    14761 cgggaacgaa aaacttattt caaaggtaat caaccagctg tttttgaggg gcgcagatgt
    14821 tatatatgaa gccctggcag acatccatgt ttcgggacat gcatgccagg aagaattgaa
    14881 actcatccac accctggtaa aaccgaaatt ttttattccc gttcatgggg aatatcgcca
    14941 tttaaagcaa catgccaacc tggccgaatc cttggggatg gcgagggaaa atatcaaagt
    15001 attagatatt ggcaacgtag tcgagtttac ccctgattca tgtaaggtaa ccggatcggt
    15061 atccgcaggc gggattttgg tggacggctt ggggataggg gatgtaggta atatagtcct
    15121 tcgggataga aggcatctat ctcaggatgg cttaatggtg atcgtagtta caatttccaa
    15181 agtttccggc ttatccgttg caggaccgga cattatatcc agaggatttg tatatgttag
    15241 agaatccgag cagttgatgg aggatgcaaa agaggttatc cgcgggattc tggaggattg
    15301 tgaagagaat caaattacgg attggggaac catgaaatat aagattaagg cgggactaag
    15361 gtcttttctc tatgagaaaa cgaagcgtaa tcctatgata ctgcccatta ttatggaaat
    15421 ataagagatg tactgcgatt gctaatgcat ttgatgttga tgttataacg gggcggatga
    15481 atgtctcccg tttctatagg cggcgagttt cgaccagtat tagacagtag cccggcgcat
    15541 cccattactt tgacttgtca aaccggatgg gcgcatatat aataggatta gattttgatt
    15601 atacatttta tggctagaac catatatgag tatccatacg tcataaaagg ttaaatttaa
    15661 ttcatacact actgaaatat gttgttttaa tgcggagtca aatcatggga atgtattcgt
    15721 tataaaaagt ttttataaca aatacagggg gcatagtgta agctaatagg tggaggatgg
    15781 atatgtcaca gaaagggaca gttttcttaa agaggatctt gagacaagta ttgatatttc
    15841 ttcttagcat cttaatagtt gcttctaccg tatacttcgg gataaatact gcctatagca
    15901 gatatattaa accggtggat gccggcaact ctagcctggt agacattgag atcccaaggg
    15961 gatcttcgct gagtaaaata tcggagattc tctatgagaa taatctcatc cgcaacaaga
    16021 tggtcttcaa gttatatgtg gacatttcca ataagacctc gagactcaag gccggaaagt
    16081 ataaactttc cagggatatg gatttggacc ggataatgga cgaactgctt accggcaatg
    16141 cggctattga tacgataaaa ataactataa tagagggttg ggatatccgc aggatagcca
    16201 gatatttgat tgaggagaag gaatatggat tttcggaaaa ggaatttata gacgccgcca
    16261 agatagaaaa ctttacggat tttgttttcc tacaggatat acccgaggag aggaagacgg
    16321 gtgaggttgg gattgcacca ctggagggtt atctttttcc ggacacatat ttggtttatg
    16381 aagatgccag ccccgaggat ataatgagaa agatgttgac ccaatttgaa aaggtctata
    16441 acgaatcggt gatggaaaga gcccaagaac ttgaaatgga tatggatgag gtagtaacct
    16501 tggcatcggt tattcagcgt gaagcccgta taactgagga attccctatg atatcagcgg
    16561 tgtttcataa tagattaaaa aaggatatgc ctctgggggc cgactcaacc atccagtttt
    16621 tggtcaacga agatcgttgg gctttcagca gcgaggaatt gaagatagat tcaccataca
    16681 acacctatga gaaccaaggg ctgcctattg gccctatatc ttcacccggc cgtctggctt
    16741 tgacatcggc gttgtttccc tacgaggagt ttatgaactc gaaaaagcct tatctatatt
    16801 ttgtgttaaa ggatcctaag acgggggaac acgtttttaa taccgattac aaccaacata
    16861 tgaaggataa aaaggaatat gaggccagtt ggaaggacat cgggaaccaa gaagattaac
    16921 gagatacaaa agataagggg gccacgaaat tgtggcctct tataacatcg gaagcaattg
    16981 catcacgcag gtttcaacga ggcgggggct ttatacccat cgcctgtttt tactaatgtg
    17041 aactcgccgc ttatagaggc gggagacatc ttgccgactc gttttattat tataggggga
    17101 aaaggttatg tcttttagaa caccggaact attggcgcct gcaggcaatc tggagaagct
    17161 gagagttgcc tttgcatacg gggcggacgc
//
LOCUS       DASRNX010000082         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_122069,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000082 DASRNX010000000
VERSION     DASRNX010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122069"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1504
                     /locus_tag="VFD89_03330"
     CDS             281..1504
                     /locus_tag="VFD89_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZJ57254.1"
                     /translation="MGENEKNVMSVDLLKVARMVSISVDLLEYGVYHHGERVAYIAYR
                     LFREMFPDKDALPTVLAGLLHDIGIDTGELKKEARNFIVEDYIISKHCEEGEMLLDKV
                     DLLKGLKPFIRHHHTRYGDIEGTKGEAVPIEAQIICLADRVEVLIDQNQYILSQKGRI
                     KGKIKDFSKDLFNPEVVRAFLRLSDTESFWLGIDNQYMLAKLWSEGFDGCSTAAEKDI
                     RQLAELCAAIVDRKSSFTHIHSKRVASIARRLGEKMYMGQRDLFILEIAGELHDIGKL
                     SIPLSILHKSGGLTEEEYRIIRQHTYHTYYLIDRLDIMDKVRDWAAFHHEKLDGSGYP
                     FHLKGSELDLGARILALADMTTALSEDRPYRKKFSGEEIMDILWKQVRNNKVDGDVVR
                     ALEKNFDYIVFTDKQ"
     gene            1609..>2895
                     /locus_tag="VFD89_03335"
     CDS             1609..>2895
                     /locus_tag="VFD89_03335"
                     /inference="COORDINATES: protein motif:HMM:NF037180.4"
                     /note="catalyzes the formation of
                     2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from
                     N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and
                     L-glutamine in purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HZJ57255.1"
                     /translation="MPNEVRRIFLEKKDQYNIEANNLYRDLKRNLGVDELDSLGIAYG
                     YDIMGLSQGEYERVCDIIFRPTHAESVYHEQLPKDAGDEVLAIQYLPGQFDQRAHAMA
                     RSIQAITLGELPLVRATRMVVLKGQLDEVDYTRIINYIVNPVESRLVSLEKPKSLKED
                     YEIPQEVDIIHGFMDASHEGLEELMEELRLAMDMDDLMFCQGYFRDVEKRDPTITEMR
                     MIDTYWSDHCRHTTFLTRLEDVEFEAGFASEWVRYVFGAYKDSRKYVYGGKDRDICLM
                     DIATIVMKEMKKRGMLEDLDESEEINACSIAVTANIDGHDEEWLVMFKNETHNHPTEI
                     EPFGGASTCLGGAIRDPLSGRSYVYQAMRITGSGDPRTHIDKTLEGKLPQIKITEGAA
                     AGYSSYGNQIGVPAGQVVEIYDPGYVAKRLELGAVVA"
BASE COUNT          862 a          548 c          760 g          725 t
ORIGIN      
        1 gaatgtttgt cgtaaaacat atagatttga agaattatat tccaatcact aaatttctag
       61 gtccaagaac ctgtaaactt gctacggggc ctgctcaaac tcgctgcgct caaacaatga
      121 ttcggcctat cctccgctgc gcgacaggtt ctaagccttt acaccatgtg atttccatat
      181 aattcttcaa ttaatgagat aagcccgaaa actatggttt attagaattt gattaaaaga
      241 agtgatgata ttgctaagaa atccgaccag ggggactatc ttgggagaga acgaaaaaaa
      301 tgtcatgtcg gtagaccttt taaaagtggc gcggatggtg tcgatatccg ttgacctatt
      361 ggaatatgga gtctatcacc acggcgaacg ggttgcctat atagcctatc ggctgtttag
      421 ggagatgttt ccggataaag atgcgctacc tacagtgttg gccggcttac ttcacgatat
      481 aggcatagac accggtgaat taaaaaagga agcacggaac tttattgtgg aagattatat
      541 aatatccaag cactgcgaag aaggagagat gctcctggat aaggtggatc ttttgaaggg
      601 gcttaagcca tttataagac accatcatac aaggtatggg gatattgaag gaaccaaggg
      661 cgaagcggtc cccatagaag cccaaataat atgcctggcg gatagggtag aggtattgat
      721 agatcaaaat caatatatcc tttcccagaa gggtaggatt aagggaaaaa taaaggattt
      781 ttctaaagat ctgttcaacc ccgaagtagt ccgtgccttt ttgaggctgt ccgatacgga
      841 atcattttgg ttgggtattg acaaccaata catgttagcc aagctgtggt ccgaaggttt
      901 tgacggttgc agcactgcgg ctgaaaagga tatacggcag ctggcggagc tgtgcgccgc
      961 catagtggac agaaagagct cctttactca tatccattct aaaagggtag cctccatagc
     1021 cagaaggttg ggggaaaaga tgtatatggg gcagagggat ttgttcattt tggagattgc
     1081 cggggagctt cacgatatag gaaaactgtc tattccccta tccattctcc ataaatccgg
     1141 tggactgact gaggaggagt atcggattat aagacagcac acttatcata cctattattt
     1201 aatcgatcga ttggatataa tggacaaggt acgggactgg gcggcttttc atcatgagaa
     1261 gctggacggt agcgggtatc ccttccattt aaagggcagt gaactggatc ttggtgccag
     1321 gattctggcg ttggccgata tgaccaccgc tttatcagag gataggccct acagaaagaa
     1381 gttttccgga gaagaaataa tggacatcct atggaagcag gttaggaata ataaggtcga
     1441 cggggatgta gtaagggccc tggaaaagaa ttttgattac atagtattta cagataaaca
     1501 atagttaaac agttcaacga aatttatcta cagatgataa attatatagt tgaagttgtt
     1561 tatctataat aaaatttagg cctatggccg aattttagga ggatcattat gcctaatgaa
     1621 gtaaggcgaa tttttttaga aaagaaggac caatacaata tagaagcgaa caacttgtac
     1681 cgggatctta agaggaacct aggggtagat gaactggatt cccttggcat agcatacggc
     1741 tatgacatca tgggcttatc ccaaggggag tatgaacggg tctgtgatat tatctttagg
     1801 cccacccatg cagagtcggt ttatcatgaa caattgccaa aggatgcggg agatgaggtc
     1861 ttggctattc aatatctccc ggggcagttc gatcaaaggg cccatgccat ggcgagatct
     1921 attcaggcta taaccttagg agagcttccc ctagtcagag ctaccagaat ggtggtatta
     1981 aaggggcaac tggatgaggt ggactatacc agaatcatca attatatagt aaacccggtg
     2041 gaatcaaggc ttgtatcctt ggaaaagcct aaatccctga aggaagatta cgaaatcccc
     2101 caagaggtgg atataatcca tgggtttatg gacgcttccc acgaagggct ggaagaatta
     2161 atggaagagc tgaggcttgc catggacatg gatgatctca tgttctgcca gggctacttt
     2221 agggatgtgg aaaagaggga tcccaccata accgagatgc gtatgataga cacctattgg
     2281 tccgatcatt gtaggcatac cacctttttg accagattag aggatgtgga atttgaggcg
     2341 gggtttgcca gtgaatgggt ccgatacgta tttggggctt ataaggactc ccggaagtat
     2401 gtatacggtg gtaaagacag ggacatctgt cttatggaca ttgcaaccat agtcatgaag
     2461 gagatgaaaa aacgtgggat gttggaggat ctggacgaat ccgaagagat caatgcatgc
     2521 agtatagccg tcaccgccaa cattgatggc catgatgagg aatggcttgt aatgttcaaa
     2581 aatgaaaccc ataaccaccc cacagagata gaaccctttg ggggtgcatc cacctgtctg
     2641 ggaggcgcca tcagagatcc actgtccgga aggtcctatg tataccaggc catgagaata
     2701 accgggagtg gggacccgag aacccatatt gataagacct tggaaggcaa gctcccgcaa
     2761 ataaagataa ccgagggcgc agccgcgggc tacagctcct atgggaatca gataggtgta
     2821 cctgcgggtc aggtggtaga gatctatgac ccaggatacg tggctaagcg tctggagctt
     2881 ggcgctgttg tagct
//
LOCUS       DASRNX010000083         4657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_439915,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000083 DASRNX010000000
VERSION     DASRNX010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439915"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFD89_03340"
     CDS             <1..229
                     /locus_tag="VFD89_03340"
                     /inference="COORDINATES: protein motif:HMM:NF022597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HZJ57256.1"
                     /translation="IRRMEMTKVLVLDFLDSASKRFSLKIPEPREDLESEEVRVAMAD
                     LIDKDPFVRTLVSIERAYIVTTTEEMLVFGA"
     gene            692..2098
                     /gene="ltrA"
                     /locus_tag="VFD89_03345"
     CDS             692..2098
                     /gene="ltrA"
                     /locus_tag="VFD89_03345"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZJ57257.1"
                     /translation="MNSKDMQRPQTTQSRGYPLKEGKETRGSMEVRSMPSALEDGRNN
                     VQRYTSNMLDMIVERGNMRKAYKRVVSNKGSHGIDGMKVDELLPFLNENWIAIKEQLL
                     GGKYKPEPVRRVEIPKPDGGIRLLGIPTVLDRLIQQAIAQVLGGVYNHTFSNSSFGFR
                     PRLGAKDAVVAAQDHMNDGYTWVVDIDLEKFFDRVNHDILMSKLESRIGDKRVLKLIR
                     RYLESGVLINGLKVSTEEGTPQGGPLSPLLANIMLDELDKELENRGHRFCRYADDCNI
                     YVKSRSAGNRVMASISNFIEIKLSLKVNESKSAVDRPWKRKFLGFSFYCSKDGIKIRI
                     HDKSIKRFREKVREITNRNKGISMEYRIYRLNQITTGWVNYFGLADAKGRMQSLDAWI
                     RRRLRACYWKQWKKIRTKHDNLVRLGTEDSKAWEFANTRKSYWRTSNSPILATTLTIK
                     YFDNIGYKRLSERYLMVH"
     gene            2497..4461
                     /locus_tag="VFD89_03350"
     CDS             2497..4461
                     /locus_tag="VFD89_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZJ57258.1"
                     /translation="MATAEKLNFERVGQVKKYIIIIVSVIILSMIGYGVYSYYQIANP
                     KVLFENPPKRVDTPNADIKEEDKPASVEVDPLLEDPFEFSERVNFLFLGLDASVERYE
                     TMDSFRTDTIMLISADFDENKVDIISIPRDSYVEIPGRERREKINAAFTRGGGLKGQG
                     FDKTIETVSHFLGGIPIHYYIGIDMNVVKDVVDIMGGVDYDVDLRVKIGERIVEKGFQ
                     HLNGQQVLDYSRTRNTGKGDIDRIDRQQKIVLAIFKELKSTKQLLKLPQYYTTVMERV
                     HSNMDIKQIAGLALFAVNMEFENIQRHTIPGGFLNMDKISYWGIHERQKGKLIKEIFG
                     VDIKIDPKNDIKYIKKELAEKEKAFKGAVAQGKEIVEKANRLIASHEKIIKDEETHRI
                     KSYISQVDHAVESGDIDTIKKVTADFKEHTIKLQSTLSQRGDAIKNAETLLTETSGKI
                     KKNQGFLEKEERDGLDKGMRDLKTAINSRDTKGINESAKALRDLSGRIFTDCQNRKEI
                     KEIKDKEQKDKEQREKEQRDKEAKDKEAAERAKAMENANAIADKTKNKMEEYGNYMKD
                     GEKAGLDKRLANLNKIIGDKNSDAKTINSASQDLKEYGDKVFSDCKQRKEDADKEDKN
                     TKGDKEDKEDKEDKKTKENQENEKDTEKNM"
BASE COUNT         1635 a          766 c         1160 g         1096 t
ORIGIN      
        1 gataaggagg atggagatga cgaaagtttt ggttttagat tttttagata gtgcatctaa
       61 aagattttca ctaaagattc ccgagccaag ggaagacctt gaatccgagg aagttcgggt
      121 tgctatggca gacttaatcg ataaggatcc ctttgtcagg acccttgtaa gcatcgaaag
      181 ggcatacatt gttactacta ctgaagaaat gcttgtcttt ggcgcataaa agaatcgtac
      241 ctatggggag gcccagcctc cccaattatt atgtgtgccc ggcatgggcg gtaacttggc
      301 ggtgagagtc cgctatgggc ttggtagtgg gaaccattag ctgaaggcaa gggtgtccat
      361 cgtgaggtgg aatctgaagg aagccctagg caaagtcccg gtctgatgca caaaaaccag
      421 atacgaggct gtttagaacg gatgagtttg cgaaacaaaa caaagtccaa cactacccga
      481 attctaatca gtatatttgg cagatatatg ggatgaaggt tatcgctctt acccggggag
      541 gtctcaggga tatgccatgg aaatgaattt tgaaatagca acccatacag tgatgtatgg
      601 ctgaaccctg agaagtcagc agaagccata gtacctacct agtcatgaat tggtagggaa
      661 gggcagaaca ttaggaggtt ttggaaatct tatgaactcg aaggatatgc aaagaccgca
      721 gacaacccaa tcaaggggct atccattgaa ggaagggaag gaaacccggg gatcaatgga
      781 agtgcgtagt atgccatcgg cgttggaaga tggaaggaac aatgtacaaa ggtacaccag
      841 taatatgctt gatatgatag ttgaaagagg aaatatgagg aaagcataca agcgtgtagt
      901 ctccaataag ggtagccatg gaatcgatgg aatgaaggta gatgaacttt taccttttct
      961 aaacgaaaac tggatagcca ttaaggaaca attactggga gggaagtata agccagagcc
     1021 agttagaagg gtagaaattc caaagccaga cggtggaata agattacttg gaatacccac
     1081 ggtgctggac agacttatac aacaagccat agcccaggtg cttggtgggg tatataatca
     1141 tacattttca aatagtagtt ttggttttag accacgcctt ggagcaaaag atgcagtagt
     1201 agccgcacaa gaccatatga atgatggata tacgtgggtg gttgacattg accttgagaa
     1261 attctttgac agggtaaatc atgatatttt aatgtctaaa ttagaaagtc ggatagggga
     1321 caaaagggtt ttaaaactta tccgaagata tttagaatca ggggtgctga taaacggact
     1381 aaaggtaagc acagaagaag gcactcccca aggcgggccc ttaagcccgt tattggcgaa
     1441 tatcatgcta gacgaattgg ataaagaact agaaaacagg gggcataggt tctgccgcta
     1501 cgcggatgat tgcaacattt atgttaagag caggtcggct ggaaacaggg taatggcaag
     1561 tataagtaac ttcattgaaa ttaagctgag tttaaaagta aatgaatcta aaagcgcagt
     1621 ggatagacca tggaagagga agttcttagg tttctccttc tattgcagta aggatggaat
     1681 taagataaga attcatgata aatccatcaa aaggttcagg gaaaaggtaa gggaaatcac
     1741 taaccggaat aagggaataa gcatggaata cagaatctat agacttaacc aaataactac
     1801 tggctgggtc aattatttcg gtttagctga tgccaaaggt agaatgcaat ccctagatgc
     1861 atggattagg cgtagactaa gggcatgtta ttggaaacaa tggaagaaaa tcagaaccaa
     1921 gcacgataat ttggttcggt tgggaaccga agatagcaag gcctgggagt tcgctaatac
     1981 aagaaagagc tattggagaa cctccaacag ccctattctt gctaccaccc ttactattaa
     2041 atactttgat aacattggct ataagcgttt atctgaacgc tatcttatgg tacattaatt
     2101 tctaatgaac cgccgtatac cgaacggtac gtacggtggt gtgggaggtc ggtagatgag
     2161 ataatcatct acctcctacc cgataaagat attctattct accgatttat tctatcgatc
     2221 cattctatta ataccattgc tgcttctaag gctgtcgaag actattgggg cctgatttgt
     2281 cagaattgta atcatattta cacataaaat caatgcatat atatggttag aatagtataa
     2341 tacctgtagg ggctaaaaga tgctcctcta aattgctttg ctgttaagtc ccgttaaaat
     2401 gctttgatat atattcggat gactaaaaga tggttaattt ggaattatta atgataaggg
     2461 tttgtagcgg caagcatcta taggtagtag gcgtttatgg caactgcaga aaagctgaat
     2521 tttgaaaggg tgggacaagt gaaaaagtat ataattatca tcgtttcggt gattattctg
     2581 tccatgatag gctatggggt ctatagctac taccagattg ccaaccccaa agtattattt
     2641 gaaaacccgc ctaaaagggt ggatactcca aacgccgata ttaaagagga agacaaaccg
     2701 gccagtgtgg aagtagaccc tttgttggag gacccatttg agttcagtga aagggttaat
     2761 tttcttttct tggggctcga tgccagtgta gagcgctacg aaaccatgga ttcctttcgc
     2821 accgatacca ttatgcttat atccgcagac ttcgatgaaa acaaggtgga tatcatatcc
     2881 atacccagag atagttatgt agagataccg gggcgagaaa ggcgtgaaaa aatcaacgcc
     2941 gcctttacac ggggaggtgg cctcaaagga caaggttttg ataagaccat agaaaccgtc
     3001 agccattttc tcggcggtat ccccatacac tactatattg ggattgatat gaacgtggtt
     3061 aaggatgtag tagatattat gggcggcgta gactacgatg tggacctgag ggtaaagata
     3121 ggcgagagaa tcgttgagaa gggttttcag catttaaacg gccaacaggt attggattat
     3181 tccaggaccc gaaatacagg caaaggtgat atagacagga tagatcgaca acaaaaaata
     3241 gtgttagcta tattcaaaga gctaaaatcc acaaagcagc tattgaagtt gccccaatac
     3301 tacacgactg taatggaaag ggtccatagc aatatggata tcaagcagat tgccggcctg
     3361 gccctttttg cggtcaatat ggaatttgag aatattcaac gccacaccat ccccgggggc
     3421 tttttaaaca tggataagat aagctattgg ggaatccatg aacggcaaaa agggaaattg
     3481 ataaaagaga tctttggggt agacattaaa atcgatccaa aaaatgatat caaatatatt
     3541 aagaaggagc ttgcagaaaa ggaaaaggca tttaagggag ccgtggccca gggcaaagag
     3601 atagtggaaa aggcaaatcg gctcattgca agccatgaaa agataataaa ggacgaggaa
     3661 actcatagga taaaatccta tatctcccag gtggatcatg cggtagagag cggagacatt
     3721 gatactataa agaaagtgac cgctgatttt aaagagcata ccataaagct acaatccacc
     3781 ctgagtcagc gcggcgacgc tataaagaat gcagaaacct tgctaacgga aacttccgga
     3841 aagatcaaaa agaaccaggg atttcttgaa aaagaagaaa gggatggctt ggacaagggg
     3901 atgagggatt taaagacagc gataaattcc agggatacaa aaggtatcaa cgaatcggcg
     3961 aaagccttga gggatcttag cggaaggata tttacggact gccaaaatag aaaagagata
     4021 aaggagataa aggacaagga acagaaagac aaagaacaga gggaaaaaga acaaagagat
     4081 aaagaagcga aggataagga ggcagccgag agagcgaagg ctatggagaa tgccaatgcc
     4141 atagcagata agaccaaaaa caagatggaa gagtatggga attatatgaa ggatggggag
     4201 aaggccgggc tggataaaag gctggccaac ctcaacaaga taataggtga taaaaatagc
     4261 gatgccaaga ccataaatag cgcatcgcaa gatttaaagg aatatgggga taaggtattc
     4321 agcgattgca aacaacgaaa agaagacgca gataaagaag ataaaaacac taaaggggac
     4381 aaggaagata aagaagataa agaggacaaa aaaactaagg agaatcaaga gaatgaaaaa
     4441 gatacagaaa aaaatatgta aaacaaagga tgtagataaa gaagacgaat gaagcagatg
     4501 agtttggttt taatcgcttc aaagggcagc cgcagagctg ccttttttat agggtcattc
     4561 ttaaaattaa tattcaatta catcaaatta attattgaca aacaataatt tccggaatat
     4621 actattaata taattattgt aaaacaataa tgggggt
//
LOCUS       DASRNX010000084         6177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_705014,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000084 DASRNX010000000
VERSION     DASRNX010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705014"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VFD89_03355"
     CDS             complement(<1..691)
                     /locus_tag="VFD89_03355"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZJ57259.1"
                     /translation="MKKIQDIIGLPLISIDGAMELGTIKDVLIDPASSKVKYLLVMDD
                     KWYLGARLVSFGDILSIGIDAVTIQGERSCRRFSEVEEAVRLAQKEVRVNHSRVFTEK
                     GQYMGSVKECYIALADGVISKCHLEGVRGPEIIEHPKIVSFGPNILVFQEGKQAGKET
                     IPSVIKAPAKNSVRMFEEKQRQFLIGRKGTRTVLDGSGNILLEEGQTLTGEILDKVKD
                     KNKIMELTINTR"
     gene            complement(783..1415)
                     /locus_tag="VFD89_03360"
     CDS             complement(783..1415)
                     /locus_tag="VFD89_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HZJ57260.1"
                     /translation="MQKSSRISEAVIRRLPKYYRYLKDMEKGGMQRISSKELSKRMGL
                     TASQIRQDFNCFGGFGQQGYGYHVSDLCSEIRKILGLHKKYNVIIVGAGNLGQALSNY
                     SGFAREGFNITAMFDANPRLIGMSIRGVQIYDIGTMGDFIINNDIRVGVICAPKDQAQ
                     SIADQMVTSGIKSIWNFAPIDVKAPDDIAVENVHLSDSLYTLTYKMNDID"
     gene            1907..3820
                     /locus_tag="VFD89_03365"
     CDS             1907..3820
                     /locus_tag="VFD89_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZJ57261.1"
                     /translation="MIVLACDKVTKSFGVNLILNQITFTVNDGSRLGVVGGNGAGKTT
                     LFNIIAGDLSVDSGEIHKHRETTMAYLPQNTVLMSHRTLWDELLGVFEALIEQEDTLR
                     RMEEKMAHYSSMDETEYSRLLSEYSRLQESFDSRGGYSYESYIRGVLIGLGFSQHEFN
                     TPIAHLSGGQKTRVALAKVLLEKPNLLLLDEPTNHLDLSAMQWLEEYLLSYPGTIMVI
                     SHDRYFLDAVCDNILEIENRAGRLYGGNYSNYIIQKSQWNAQREKEYIAQQKEISRQE
                     GIIERYRSFNREKSIRAAESRQKRLDKMERLEKIHDMDRIDFSFGIDRQSGRDVLSVE
                     SLSKAFGDKSLFEDISFDIRLGDRVALIGPNGCGKSTLLKIILDEMPPSSGIVKKGVG
                     VEIGYYDQELNSLNPQNMVIEELWDDFPQMRETEIRNALAARLFKGDSVYKSVASLSG
                     GEKGHLILTKLVLSNNNFLLLDEPTNHLDMASREELEQALTEYPGTILMVSHDRYFLN
                     KIADRVIEMESGDITEYLGNYDDYVNKKGALDALTGRNEKIPDITRTASREERRRERM
                     ERERAKEEKERLENLEVRIHQLEDEIKDLEVMMADPGLYDDVNKMLEVQQRYELLKDE
                     LEGVYSKWMNLAK"
     gene            complement(4176..6101)
                     /locus_tag="VFD89_03370"
     CDS             complement(4176..6101)
                     /locus_tag="VFD89_03370"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HZJ57262.1"
                     /translation="MGKNIVQKIIESHLVSGEMVPGQEISIKIDQTLTQDSTGTMAYL
                     QFEAMGVPRVKTKKSVAYIDHNTLQAGFENADDHKYIQTIASKHGIYFSRPGNGICHQ
                     VHLERFSRPGWTLLGSDSHTPTGGGAGMLAIGAGGLDVAVAMAGGAYYLTMPKVCRVV
                     LKGKLRPWVAAKDIILEVLRVLSVKGGVGKVMEYAGEGVSTLTVPERATITNMGAELG
                     ATTSIFPSDEETRKFFIAQGREGEWTELKADEDAQYDEEIEIDLSALEPLTAKPHSPD
                     DVDTVENVGKIKVDQVAIGSCTNSSYLDLMKVAEILRGKTVHPDVSLVIAPGSKQVYT
                     MLAKNGALADMIDAGARILESACGPCIGMGQAPASDAVSVRSFNRNFYGRSGTPSAKV
                     YLTSPEVAAITAITGYLTDPRSIGEYRDIEMPAEFVINDNSIISPAEDGDEVEVVRGP
                     NIKPFPINTPLAGSEGGVVLLKMEDNITTDHIMPSNAKLLPYRSNIPHLSDYCLVPVD
                     PDFPKRAKEAGGGFLIAGHNYGQGSSREHAALVPLYLGIKGVIAKSFARIHMSNLINS
                     GILPLTFAREEDYEAIDEGDELVIEDAMEKVKSGDRLTVRNVTKGKDIEVVLSLSHRL
                     KDIILAGGMLNYTKQNA"
BASE COUNT         1603 a         1508 c         1317 g         1749 t
ORIGIN      
        1 atcttgtatt tatggtcaac tccattatct tgttcttatc ctttacctta tccaatatct
       61 caccggtcaa tgtctggcct tcttcaagaa gaatgtttcc cgatccgtcc aacacggtcc
      121 tggttccctt cctccctatt aaaaactgcc tttgtttttc ttcaaacatc cttacgctat
      181 ttttagcggg ggcctttata acgctgggga tcgtctcttt tcccgcctgc ttaccttctt
      241 gaaacaccag gatattcggg ccaaaggata caattttagg gtgctctatt atttccgggc
      301 cgcggacccc ctcaagatga catttactga taaccccatc cgccaaggct atatagcatt
      361 ccttgactga tcccatatat tgtccttttt cggtaaatac cctggaatgg ttaaccctga
      421 cttccttttg ggccagcctg accgcttcct caacttcaga aaatctcctg caacttcttt
      481 caccttgaat cgtaactgca tcgataccaa tggatagtat atcgccgaag gaaacgagtc
      541 ttgctccaag gtaccattta tcgtccataa ccagaaggta ctttactttg ctgctggccg
      601 gatcgattaa aacatccttt atagtaccca gctccatagc cccatcaata ctgattaagg
      661 gtaaccctat aatatcctgt atttttttca tcttaacccc accgcctaaa tatttcgctg
      721 tcctgcttgc tttgccccat acacaaaata tcgaaaccta ttacttcaat aaatgcattt
      781 tatcaatcga tatcattcat cttatacgtc agggtataca agctgtcgct taggtgtaca
      841 ttttctaccg caatatcatc cggagccttt acatcaatgg gcgcaaagtt ccaaatactc
      901 tttataccgc tggtgaccat ctgatccgct attgattggg cctgatcctt tggtgcgcat
      961 attacgccca cccttatatc attgttgatt ataaagtctc ccattgttcc gatatcatag
     1021 atctgtaccc ccctgatgga cataccaatc aggcgggggt ttgcatcaaa catggctgtt
     1081 atgttaaatc cttcccttgc aaatcctgag tagttggaca aagcctgtcc cagattccct
     1141 gcaccaacaa ttatgacatt gtatttctta tgaagcccca agatcttcct tatctcactg
     1201 cacaaatcgc ttacatgata cccgtatccc tgctgcccga acccgccaaa acaattaaaa
     1261 tcttggcgga tttgggaggc cgtaaggccc atcctcttac tgagttcctt cgatgaaatc
     1321 ctctgcattc cgcccttttc catatccttt aagtatcgat aatacttggg caaccgtcgg
     1381 ataaccgctt ccgaaattct gctacttttt tgcatgaaca caccctccct ggattacgct
     1441 catattctag gattgctaat agtctgtcct acattttttc ataaatatat caaggagccc
     1501 gactaatgac tttcactttt tatgattttc tgctatcatt aaaactatgc cggatgcatt
     1561 agaatgcgat ttaataacat cggacttatt tggcataaag ctatttctgt gattaaagtg
     1621 ttatcgaaaa tgcattccca cggatgagtc cttttcgtaa catatctgat tatatcacaa
     1681 aaagtattta tttagaccgt ttatatttga ttcgccgcaa aaactatggc taaatcggat
     1741 tatgcagcgt aacggcgttt aatttagtgt gggctttcgc cccaagcgct actatagaag
     1801 taaccggtgg gttattgtta taataaatat accgccggca gaataatcgt atattgctta
     1861 ataatttgtt cattataatt tgttcattat aatcgggagg tcaaagatga tagttctagc
     1921 ctgtgataag gtaaccaaat ccttcggcgt taatttaatt ttaaaccaaa taacttttac
     1981 tgtcaatgat ggatctcgcc tgggggttgt agggggcaac ggtgccggaa agaccaccct
     2041 attcaatata attgccgggg atttatcggt agacagcggc gaaatccaca aacacaggga
     2101 gaccaccatg gcatacttac cccagaatac ggtgcttatg tcccacagaa ccctttggga
     2161 cgagcttttg ggggtatttg aagccctaat agaacaggaa gacaccctca ggcggatgga
     2221 agaaaagatg gcccattaca gttccatgga cgaaacggaa tattccaggc tcttaagtga
     2281 atattccagg ctgcaggaat cctttgatag tcggggcggc tacagttacg agagctatat
     2341 aaggggtgtt ctaataggcc ttggattctc ccagcatgaa tttaataccc ccatcgcaca
     2401 ccttagcggc ggccaaaaga ccagggtagc cctcgctaag gtgcttcttg aaaagcccaa
     2461 tctcctgctc ctggacgagc ccaccaacca cctggaccta agtgccatgc aatggctgga
     2521 ggaatacctt ctatcctatc ccggaactat aatggtcatc tcccatgaca gatatttttt
     2581 agatgcggtc tgtgataata tactggagat agaaaataga gccggtcgtc tatacggcgg
     2641 aaactattcc aattatatca tccaaaaatc tcagtggaat gctcaacggg aaaaggagta
     2701 tatagcgcag caaaaggaga tttcccgcca agaggggatt attgaaaggt accgttcctt
     2761 caatagggaa aaaagcatca gagctgccga aagccggcag aagagactgg ataagatgga
     2821 aaggctggaa aagattcatg atatggatcg tatagacttt tcctttggta tagacaggca
     2881 gagcgggcgg gacgtgttat cggtagagtc tttatccaag gcctttggtg ataaatcttt
     2941 atttgaagat atctcctttg atatccgcct tggagataga gtggccctta tcggccctaa
     3001 cggatgcgga aaatccaccc tacttaagat aatcctggat gaaatgcccc cgtcctcggg
     3061 tattgtgaag aagggcgtgg gggttgaaat cggatattat gaccaggagc taaatagcct
     3121 caacccgcaa aatatggtca tagaagaatt gtgggacgat ttcccccaaa tgagggaaac
     3181 cgagatacga aatgccttgg cggccaggct ctttaagggc gattctgtgt ataaatccgt
     3241 tgcctccctc agcggcggtg aaaaagggca tcttatcctg accaagctgg tcctatccaa
     3301 taataatttt cttctgttgg atgaacccac caaccacctg gacatggcat cgcgggaaga
     3361 actggaacaa gccctgacag aataccccgg tactattctt atggtatctc atgaccgata
     3421 ctttttaaac aagatagccg atcgggtcat cgaaatggaa tccggggaca tcacagaata
     3481 cctaggaaat tatgatgact atgtaaacaa aaaaggagcc ctagatgccc tgacaggacg
     3541 taatgaaaaa atccccgata ttacaaggac cgccagccgc gaggaacgcc gcagggagcg
     3601 catggaaagg gaaagagcca aggaagaaaa ggagcgtctt gagaaccttg aggttcggat
     3661 acatcagttg gaagatgaga taaaggatct ggaagtgatg atggcggatc cgggcctata
     3721 tgacgatgtt aataagatgc tggaggttca gcaaaggtat gagctgctaa aggatgagct
     3781 ggagggggtt tattctaagt ggatgaacct agccaagtga tgccgattat ttatggataa
     3841 acaacagatc aactaaattt atctgtaagt gataagtttt atagttggag ttgtttatct
     3901 atatctgatg attattaagc aaaaattaat tagagggagc ggattctgac cgctccctct
     3961 aatttaattc gttgtgcatt tagacatata taggtgatca ctattgaacg ctatttcatc
     4021 gctgatttag ggaaactaag gcttttccca tttaaaaacc ttatcgtttc caatcaggcg
     4081 tcttgcttga ttgcatagga ggatggttca ttccggcatc ttctaaaacc ccaatcagcg
     4141 aattcatacg cctttcttat tttttctagg ccatcttatg cattctgctt tgtataatta
     4201 agcatcccgc cggccaggat gatatccttc agcctgtggg atagggatag gacaacttcg
     4261 atatccttgc ccttggttac atttcttacc gtgagcctgt caccggattt aaccttctcc
     4321 atagcatcct ctatgactag ctcgtcgccc tcgtctatcg cctcgtagtc ctcttctcgg
     4381 gcaaaggtca agggaagtat cccggaattt ataaggtttg acatatgaat ccttgcaaag
     4441 gatttggcta taaccccttt tatgcccaga tataggggta caagggccgc atgctccctg
     4501 ctggaaccct ggccgtagtt atggcccgct atcaaaaagc cgcctccggc ttccttggcc
     4561 ctctttggaa aatccggatc cacaggtact aggcaataat ccgacagatg cggaatgttg
     4621 gatctataag gaagcagctt tgcgtttgaa ggcataatat gatcagtggt aatattgtcc
     4681 tccatcttta ggagtacgac ccccccctcg ctgccggcca ggggcgtatt gatggggaag
     4741 ggctttatgt tgggtcccct taccacctct acctcatccc cgtcctccgc aggcgatatt
     4801 atggagttat cgtttatgac aaattctgca ggcatctcta tatccctata ttccccgatg
     4861 cttctgggat ccgtcagata ccccgtaatt gccgttatag ctgctacctc cgggcttgta
     4921 aggtatacct tggcgctggg agttccgctt cgtccataga agttacggtt aaagctcctt
     4981 acggataccg catcggatgc aggggcctgt cccatgccta tacaagggcc gcaggcgctc
     5041 tctaatatcc tggctccggc gtctatcata tccgccaatg ccccgttctt agccagcatg
     5101 gtatagacct gcttagagcc gggtgcaata actaaactaa cgtccgggtg cacggttttt
     5161 cctcttaata tttcggcaac cttcataaga tcaaggtaag aggagttggt acagctgcct
     5221 atagctacct ggtccacctt aatcttgccc acgttttcga ctgtatctac atcatcaggg
     5281 ctatggggtt tggccgtcag tggctccaaa gcgctcaggt caatttcgat ctcttcatcg
     5341 tattgagcat cctcatccgc ctttaattcc gtccattccc cttcccttcc ctgtgctatg
     5401 aaaaacttcc tagtttcctc atcgctgggg aatatggatg tggtagcccc cagctcggct
     5461 cccatgtttg taatggttgc cctttccgga actgtcaggg tggatacccc ttctcctgca
     5521 tattccatga ccttacccac cccgccctta acggacagga cccttaggac ctctaaaatt
     5581 atgtcttttg cggcaaccca gggccttaat ttgcccttta ataccacgcg gcaaaccttg
     5641 ggcatggtaa gatagtatgc gcctcctgcc atggccacgg ccacgtccaa tccccccgcg
     5701 cctatggcca gcattccggc accaccaccg gttggagtgt ggctgtcgga acctaaaagg
     5761 gtccagccgg gccttgaaaa cctctcaagg tgcacctggt gacatatgcc gtttccgggc
     5821 cttgagaaat atatcccatg tttggatgcg atggtctgaa tatacttatg atcatcagca
     5881 ttttcaaacc cggcctgaag ggtattgtga tcgatatatg caacggactt cttggtcttt
     5941 acccttggta cccccatggc ctcgaactgc agataagcca tggtcccggt ggaatcttgg
     6001 gttagggttt gatctatttt tatggatatt tcctgccccg gtaccatctc acccgatacc
     6061 agatggcttt ctattatttt ttgaactatg ttcttcccca tattaaacct cctacattcc
     6121 tactatcaac agcccattgt ccatacctga tagaaagata atgaaccgat acagtag
//
LOCUS       DASRNX010000085         4916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_546407,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000085 DASRNX010000000
VERSION     DASRNX010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546407"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..995
                     /gene="era"
                     /locus_tag="VFD89_03375"
     CDS             99..995
                     /gene="era"
                     /locus_tag="VFD89_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019242631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZJ57263.1"
                     /translation="MDKGFRSGFVAVIGRSNVGKSTLLNTIIGQKVAIVSDKPQTTRN
                     QIHCVLTREDYQMIFIDTPGIHRARNKLGEYMVKTAVNTLNEVDLVLLVLDIANGIGI
                     GDKKIMEMLRGIKSPVVVALNKADRLTPEEVDKVMEEFKSNFQFHNYLPISALYGTNL
                     DQLESRILDYLEEGPKYYPDDMVTDQPERVIIAELIREKALDLLREEIPHGIGVEITS
                     INERQDQELMDIYATIFVEKKSHKGMVIGKRGQMLKEIGQRARKDIEGLLGIQVYLEL
                     WIKITEDWRNSPRALRDLGYNR"
     gene            1354..2124
                     /gene="recO"
                     /locus_tag="VFD89_03380"
     CDS             1354..2124
                     /gene="recO"
                     /locus_tag="VFD89_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZJ57264.1"
                     /translation="MKEITRHLPTKGVVLRYTNFKEADRMLTLFSPELGKISVLARGC
                     RKAKSRFLAATELFCFGEYTLYRRGDFHIMTQASILDSFYEIRNDVDKLIYASYVLDL
                     TEEVVSPGQGDTHLFYLLLQTLSYLCYSDLDSEGITRVFEIKLLDHIGYRPVLDRCIV
                     CESDKGPYHFDVEKGGLICSNCYNKNRIGNNIQMGTIKTLEHILGMDMNRINILKIPH
                     TIREELDTILRAYIEERIDKKLKTRQFIDEFKGKNRKN"
     gene            2252..4879
                     /gene="ppdK"
                     /locus_tag="VFD89_03385"
     CDS             2252..4879
                     /gene="ppdK"
                     /locus_tag="VFD89_03385"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZJ57265.1"
                     /translation="MSHKYVYLFSEGDATMRNLLGGKGANLSEMINLGLPVPQGFTVT
                     TEACTKYYEDDRVIAQEIVDQIHDAMLELEKIAAKRFGDLDNPFLVSVRSGARASMPG
                     MMDTVLNLGLNDDAVEGLAKLTGNVRFAYDSYRRFIQMFSDVVMEIDKSNFDEIMDKI
                     KEENGIAEDSELTAENFKEIVKGFKELYRSIRGEDFPQDPKAQLLASVEAVFRSWNNP
                     RAIVYRRMNDIPGDWGTAVNIQAMVFGNMGEDCGTGVAFTRNPATGENKLYGEYLINA
                     QGEDVVAGIRTPEPIDKLEEVMPEIYQEFADIAERLEKHYKDMQDMEFTIEKGKLYML
                     QTRSGKRTAAAALKVAVDLVDEGLITKKEALLQVDPRQLDALLHPTFEPKALNAATPI
                     ARALPASPGAATGHIYFTAEAAAEAAKAGQKVILVRQETSPEDIEGMYAAEGILTSRG
                     GMTSHAAVVARGIGTCCVAGCGDALINEAAKNMIIGDKTYQEGDFISLDGSTGSVYGE
                     ELPTEEPTLTGDFSTLMEWADEFRTLDIRTNADTPRDAQQALDFGAQGIGLCRTEHMF
                     FEEDRIPAIREMIVARSEDERRKALAKLLPLQKSDFKGIYEVMEDRAVTIRLLDPPLH
                     EFLPHADSDIRALATDMGLTFEELKSTIEGLQEVNPMLGHRGLRLAVTYPEIAEMQTR
                     AIMEAAVEVRQEKGFNILPEIMIPLVGEVKELKYVKDVVVRTADAVLEEKGVKVDYEV
                     GTMIEVPRATLLADEIAREADFFSFGTNDLTQMTFGFSRDDAAKFLDDYYEKQIFESD
                     PFARIDQKGVGKLMKMAVELGRAGRSDIKLGICGEHGGDPSSIEFCHSMGLDYVSCSP
                     YRVPIARLAAAQAAIKDNK"
BASE COUNT         1487 a          961 c         1250 g         1218 t
ORIGIN      
        1 gctgcgaacc aggttctaag tctttacacg atgtgaactc caaatagaaa aaacgcttca
       61 attgttttta atatatgcta aacttaaagg atgataaaat ggataaggga ttcagatctg
      121 gttttgttgc ggtaataggg cggtccaatg tgggtaagtc cactctgctt aacaccataa
      181 tcgggcaaaa ggttgccatc gtttcggaca aaccccagac cacaagaaat caaatacatt
      241 gcgttttgac ccgtgaggac tatcagatga ttttcatcga tacacccggt atacaccggg
      301 cgaggaataa actgggcgaa tatatggtga aaaccgccgt aaacaccttg aatgaggtag
      361 atttggtatt attggtattg gatatagcca atggtatcgg gatcggtgac aagaagataa
      421 tggagatgct ccggggtatt aaatcccctg tagtagttgc cctcaacaag gcggaccgac
      481 tgactccgga agaagtggat aaagtgatgg aagagtttaa atcgaatttc caatttcata
      541 attatctgcc gatatccgca ctttatggaa caaatcttga tcagctggag agtaggattt
      601 tagactactt agaagagggg cctaaatact atccagatga tatggtcacc gaccagcctg
      661 agcgggtgat aatagccgag ctcatcaggg agaaggcttt ggatttatta agggaggaga
      721 ttccccacgg catcggagtg gagataacca gcattaatga gcgtcaagat caggaattaa
      781 tggatatcta cgctaccata tttgtggaaa agaaatccca taagggtatg gttataggca
      841 aaaggggaca gatgcttaaa gagataggtc aaagggcgcg gaaggatatc gagggcctat
      901 tgggcattca agtatacctg gagttatgga taaagatcac ggaggattgg agaaacagcc
      961 ccagagccct tagggattta ggttataacc gataaagtga aatcatccac ttttcaatag
     1021 tccattacct ttttcacata gcaagacaat gaatcgaagc gatacgtaaa tttaaaaatg
     1081 tcgtttatta tcgatttcgg attattttac atcgaatata gtagcttctt gagggaaagg
     1141 ctaatataaa atcaacaagg aactctatgc cctgcagggt gcataacgaa ttgtttatcc
     1201 accgggctac acctatttgc tctatatatg ttgaaaatac aggggagggt tataaggtga
     1261 aggaattgat ctggggtttt tttacaaaga cgggttctgt ttcgacttat ctgctatata
     1321 aagatatgga taacgatggg gtgagggaac aaaatgaagg agattacgag acacctgccc
     1381 accaaagggg tggtgctacg ctacaccaac tttaaggagg cagaccgaat gctcacgctg
     1441 ttttcgccgg agctgggcaa gatatctgta ttagccaggg gttgcagaaa agcaaaaagc
     1501 cgctttcttg ccgctacgga gttattctgc tttggggaat ataccctata tcgcagggga
     1561 gatttccata taatgaccca agcgagcatt cttgactctt tctacgaaat tcgaaatgat
     1621 gtggacaagc tcatctatgc cagttatgtt ctggatttaa cggaagaggt ggtcagtccc
     1681 ggacaggggg atacccacct cttttatctg cttttgcaaa ctctgtccta cttatgctat
     1741 tcagatctgg attctgaagg gataacccgt gtttttgaaa taaagctttt agatcatatc
     1801 ggctatcgtc ctgtattgga cagatgtata gtttgtgagt ccgacaaagg cccgtatcat
     1861 tttgatgttg aaaagggcgg cttgatatgc tccaattgtt ataacaagaa tagaattgga
     1921 aacaatattc aaatggggac aattaaaacc ttggagcaca ttttgggtat ggacatgaac
     1981 aggataaaca ttctaaagat tccgcacacc ataagggaag agctggatac tatcttaaga
     2041 gcatatattg aggagcgaat cgataaaaaa ctaaagacac ggcaatttat agacgaattc
     2101 aagggcaaaa acaggaaaaa ctaaaaagat gtagaatatt attgaccagg ggcttaaaat
     2161 tgtataagat aatagggcgt aaaaataaga caataggcgt gaaggataag ccctattttc
     2221 atattaaata catattaagg aggacggatg tatgtcacat aagtacgtat acctttttag
     2281 cgaaggcgat gccaccatga gaaatctttt aggtggcaag ggggcaaact tatcggaaat
     2341 gataaatctt ggattgcctg tgccccaagg attcactgtt accactgagg catgcacgaa
     2401 atactacgag gatgaccgcg ttattgcgca ggaaatcgtg gaccaaatcc atgatgcgat
     2461 gttggagctg gagaaaatag ctgccaaaag attcggtgat cttgataatc ccttcctcgt
     2521 atcggtgagg tccggggcaa gagcgtcgat gcctggcatg atggacacgg tgttaaattt
     2581 ggggttgaac gatgatgccg tagaaggatt ggcaaaatta acgggtaatg tacggtttgc
     2641 ctatgatagc tatcgaaggt tcattcagat gttttccgat gttgtaatgg agatcgataa
     2701 atccaatttt gatgaaataa tggacaagat caaggaagag aacggaatag ccgaagattc
     2761 cgaattgacc gcggaaaatt ttaaggaaat tgtaaaggga ttcaaagaac tatataggtc
     2821 tatcaggggt gaagattttc cccaggaccc caaggcccaa ttgttagcat ccgttgaggc
     2881 ggttttccgc tcttggaata atcccagggc tatagtctac agaaggatga acgatatacc
     2941 cggagattgg ggcaccgctg ttaacattca ggctatggta tttggaaata tgggtgagga
     3001 ttgcggcacc ggcgtagcat tcaccagaaa tccggctaca ggcgagaaca aactctacgg
     3061 cgaatatctg ataaatgccc aaggcgagga cgtagttgcc ggtatcagaa cccccgaacc
     3121 catagataaa ctggaagagg taatgccgga aatttatcag gaatttgctg acattgccga
     3181 aaggttagaa aaacactaca aggatatgca ggatatggaa tttaccatag aaaagggtaa
     3241 actctatatg ctccagacca ggagcggtaa aagaaccgca gctgctgcgc taaaagtagc
     3301 agtagacttg gtggatgaag gccttataac aaagaaggaa gccttgttac aggttgaccc
     3361 tagacaattg gatgccttat tacatccaac ctttgaaccc aaggctctta atgcagctac
     3421 ccctatcgct agggctctcc ctgcttctcc gggtgctgct accggccata tatactttac
     3481 ggcagaagcc gccgcagagg ctgcaaaggc cggacaaaag gttatcctgg tacgtcagga
     3541 gacttcaccg gaggatatag aaggcatgta tgccgctgaa ggtatattaa cttcccgtgg
     3601 cggtatgaca tcccatgctg cagtcgttgc cagaggtatt gggacatgct gcgtagcagg
     3661 gtgcggtgat gctttgatca atgaggccgc caagaatatg ataataggcg ataaaaccta
     3721 ccaagagggc gactttatct ccttagacgg gtccactgga agtgtctacg gtgaagaact
     3781 gcccaccgag gaacctactc ttacggggga tttttctact ctaatggagt gggccgatga
     3841 atttagaact ctcgatattc gaaccaacgc tgataccccc agggatgccc agcaggcttt
     3901 ggattttggg gcccagggta ttggattgtg tcgtacagag catatgttct ttgaagagga
     3961 cagaattccc gccatccgcg agatgatcgt ggccagaagc gaggacgaga gaagaaaagc
     4021 tctggcaaag ctgctgcccc tacaaaagtc cgattttaag ggcatttatg aagttatgga
     4081 agacagggct gtaacaatac gcctcttaga tccgcctctt catgagtttt taccccatgc
     4141 cgattcggat ataagggctt tggctacaga tatgggtcta acctttgagg agctaaaatc
     4201 caccatcgaa ggactccagg aagtaaaccc catgctggga caccggggcc tgcggttggc
     4261 agttacctat ccggaaatcg ccgagatgca gactagggcc atcatggaag ccgccgtcga
     4321 ggtaaggcaa gaaaaaggat tcaatattct tccggaaatt atgataccct tggttggcga
     4381 agtcaaggaa ctaaagtacg ttaaggatgt ggtagtaagg actgccgatg ctgtattgga
     4441 agaaaagggt gttaaggtcg actacgaagt gggaaccatg attgaggtcc ccagagcaac
     4501 cctcttggca gacgagattg caagggaagc tgatttcttc tccttcggta ccaacgattt
     4561 aacccagatg acctttggct tctccaggga tgacgcagcc aagttcctag acgattatta
     4621 cgagaagcag atcttcgaaa gcgatccctt tgcccgcata gaccaaaagg gcgtaggcaa
     4681 gcttatgaag atggcagttg aattaggaag ggcaggccgc tcagatatca aacttggaat
     4741 ctgcggagag cacggtgggg accccagttc aatcgagttc tgccatagta tgggccttga
     4801 ttatgtttcc tgctccccct atcgcgtacc cattgcacga ttggcagcag cccaggcagc
     4861 aattaaagac aacaagtaat atgatgagat gttcacataa tttaaggcgt tttaaa
//
LOCUS       DASRNX010000086         5016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_705186,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000086 DASRNX010000000
VERSION     DASRNX010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705186"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..912)
                     /locus_tag="VFD89_03390"
     CDS             complement(274..912)
                     /locus_tag="VFD89_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HZJ57266.1"
                     /translation="MTIWGIFISAFLIGFSGAMMPGPMLGVTIEGSLKRGYSAGPLVV
                     LGHGILEFALVFAMAFGLKDFFSNPIIAGLIGLFGGGFLAWMGYDMIKACLKKTVSLE
                     DQGNGGMGQRNLVLAGIVVSATNPYFILWWASTGMESIRQAYVLGVLGVLVFFFGHIL
                     SDFTWYVAISTAFSRGKKLMNDTVYRWVILCLGAFILIFSIKFIIGGWQMLF"
     gene            complement(1245..3905)
                     /locus_tag="VFD89_03395"
     CDS             complement(1245..3905)
                     /locus_tag="VFD89_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 78 protein"
                     /protein_id="HZJ57267.1"
                     /translation="MLKTIGLRCEYLDNPIGMGETNPRFGWILDSDNRNVVQESYRLQ
                     VSMGEDFTDPIWDTGIIDSSESVHVEYAGPKLESSARYFYRVKVSDNHKNESPWSDAA
                     FFETGILDASEWKAAFISPEGEDEGASSKGKLLRREFNLDGEIEFARVYATSLGMYEL
                     YINGAQVGDGLLTPGWTDYRERLQYQTYDVTELLKAGDNAIGASLGIGWYKGDLAGWV
                     GRRNVYGSQMGLLLQLLVRYTDGREAIVVTDDSWKASESPVIYSELYHGETYDARREQ
                     QGWDLPRFDDADWSNVWVMDKDISTVVPQDGVMIRKREVLKPISMFKTPKDELVIDFG
                     QNMSGWVKFSAVGKAGDRVVLKHAEVLDAEGNFYTENLRAAKSRIEYILKEEGTETYE
                     PHFTFQGFRYVMIEEYPGDVDLEDFEAVVIHSDIERTGDFSCSDELINKLHHNILWGL
                     KGNFVDIPTDCPQRDERLGWTGDAQVFMKAACYLKDVRVFFKKWFRDIRAGQLGDGGI
                     PYVIPDVLTDTTEEGLVGENHSATGWGDAAVICPWTLYVTYGDKRILEEQYDSMKAWI
                     EYIRSRAQDRVLWNTGFHFGDWVALDAKEGSYFGATPNDLTATAYYAYSTRILVRAAG
                     ILGKDKDAEEYSRLHEEIVKAFRNEFFTPNGRLAAPTQTSHILSLMFGLVPKEHMDRT
                     IEGLIKLLDENDGHLVTGFLGTPYFCHVLSRNGKLKEAYDLLLKEDYPSWLYQVKMGA
                     TTIWEHWDGIKPDGTMWSPDMNSFNHYAYGAIADWLYGVVAGLDTDPENPGFKRIIMK
                     PQPGGGLTFAKAEYRSVYGKASIKWAIENGNMRVDVSVPHNTTAHLVLPDADAADVVG
                     EGADFIPCSGGCEAEIGSGTYSFSYPYVGG"
     gene            complement(4134..>5016)
                     /locus_tag="VFD89_03400"
     CDS             complement(4134..>5016)
                     /locus_tag="VFD89_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HZJ57268.1"
                     /translation="NNYSEEGAPWHIRLDFPGPHLPCRPSEDFARMYKAEDIPPWASF
                     KEDFHNKPYIQKQQLYSWDIEDYTWEDWRDTVALYYATITEIDHAIGKVLDRLEELGQ
                     AENTIVIYTADHGDMCGGHRMIDKHYILYDDVVKVPLVIRWPGVIPPSSVRDEFICHF
                     LDIPPTILEVLGQSIPDSYSGRSMLNLLKGQKAEGWRTEAVSTYNGQQFGLYTQRMIR
                     NNSWKYIWNTTDIDELYDLEKDPDELYNLMGQEKYMPIVTELRKRLYEILLEEGDGLV
                     KTHWMRNQLINNKKIDI"
BASE COUNT         1292 a         1350 c          990 g         1384 t
ORIGIN      
        1 gataaataac tccaaccata aaatttatct tcataaaatt gcatagaatc gctgcaatta
       61 tcatgccgat aaatttaatt gatctattgt ctatctacac atccttttgc cttatgtccc
      121 acattgggtc cgtttttgaa aatatatccg caaacgttga taattcaact ttttgaaact
      181 ccgactgatc ggttatttgt ttagtgatac ccgaattgct taatgtgatg gggccgcata
      241 tgtaatgcag ccccccagag tacagggtga ttactaaaac agcatctgcc acccgcctat
      301 tataaactta attgaaaata tcagtataaa agcgcccaga cacaggataa cccacctgta
      361 aacggtatcg ttcatcagtt ttttacctct ggaaaatgcc gtggatattg caacatacca
      421 tgtaaaatcc gataggatat gcccgaagaa aaagaccaga acacccaata cgcccaaaac
      481 gtaagcttgg cgtatggact ccatccccgt agaagcccac catagaataa aataggggtt
      541 tgttgcgctt actactatgc ctgctaggac cagatttctt tggcccatac ccccatttcc
      601 ttgatcctct agggaaaccg tcttttttag gcaggccttt atcatatcgt agcccatcca
      661 tgcaagaaaa cccccgccaa agagccctat cagacccgct atgatggggt ttgaaaagaa
      721 atcctttagg ccaaaggcca tggcaaaaac aagggcgaat tccaggattc cgtgccctaa
      781 gaccaccaag ggacccgccg agtatcccct cttaaggctc ccttcgatgg taacccccag
      841 catgggaccc ggcatcatgg ctccggaaaa acctattaaa aatgcgctga taaaaatccc
      901 ccaaatggtc aactgcctct accccctgtt gataatcaat tcaaaagtct tggaatctaa
      961 tttgttatac tatatatgtt acgaataaga cctatcaata gatcaatgta gttttcgtaa
     1021 catataaatc aaggaaaatg ttttacgata tctgaaacat aatgtaagca gtttatcgta
     1081 aaacatatag ttaatttaat gcgttgtgct agttcatcaa ctcactgtgt aataaagata
     1141 atgaactgtt gaaaagcgga tgtagcgttc aatagtcatt accgacatgt ttaactagca
     1201 caacaggtta acttaataca gctttgcgct cattaaccta tacttcatcc ccccacatag
     1261 ggatagctaa agctgtaggt cccggagcca atctccgctt cgcatccacc ggagcaggga
     1321 atgaaatcag ccccctcgcc gactacatcc gctgcatcgg catcgggtaa aaccagatga
     1381 gcagtagtat tatggggtac cgatacgtca accctcatgt ttccgttctc tatggcccat
     1441 ttgatcgaag ccttcccata tacgctcctg tattcagcct tagcaaaggt caatccccca
     1501 ccgggctggg gtttcatgat gatgcgctta aacccgggat tttcagggtc tgtatcaaga
     1561 cctgcaacta ccccatagag ccaatcggct atagcaccgt aggcatagtg gttaaaggag
     1621 ttcatatccg ggctccacat ggttccgtcg ggtttgatgc catcccaatg ctcccatatg
     1681 gtggtggcac ccatctttac ctgatacaac caggacgggt aatcctcctt tagtaagaga
     1741 tcgtaggcct ctttgagttt cccgttcctg ctcagcacat ggcaaaaata cggtgtaccc
     1801 agaaaacccg ttaccaaatg tccgtcattt tcgtccaata attttataag cccctctatg
     1861 gtgcgatcca tatgctcttt cggtaccaat ccaaacatca gagacaggat gtgagaggtc
     1921 tgagtagggg cagccagcct tccgttgggg gtaaagaatt cattcctaaa ggccttaact
     1981 atttcctcat gaagccttga atattcctct gcatccttgt ccttccctag tatcccggcc
     2041 gcccttacca aaatcctcgt tgaataggca taataggccg ttgccgtgag atcgttggga
     2101 gtagccccaa aatagctgcc ctctttggca tctaaggcca cccaatctcc aaaatggaat
     2161 cccgtattcc aaaggacccg atcctgtgcc ctactcctaa tgtattctat ccaggccttc
     2221 atgctatcat attgttcctc caggatccgc ttatcgccat aggttacata aagggtccag
     2281 gggcagataa ctgccgcatc gccccacccc gtggcggaat ggttttcgcc taccagtcct
     2341 tcctcggtgg tatccgtcaa gacatcgggg ataacatagg gtatcccccc gtctcccagc
     2401 tgcccagccc ttatatccct aaaccacttt ttaaagaaaa cccttacatc ctttaggtaa
     2461 caggcggcct tcataaatac ctgggcgtcc ccggtccagc cgagcctttc gtccctttgg
     2521 ggacaatccg tgggtatgtc cacaaaatta cctttaagcc cccataggat attatggtgg
     2581 agcttgttaa taagctcgtc ggaacaggaa aaatcccccg tcctttctat atccgagtgg
     2641 attaccacgg cttcaaagtc ttctagatcc acatccccgg gatattcttc gatcattaca
     2701 tacctaaacc cctgaaaggt aaaatggggc tcataggttt ctgtgccctc ttcctttagg
     2761 atatactcta tcctgctctt tgcggcgcgg agattttccg tgtagaagtt gccttcggca
     2821 tctaagacct cggcatgctt taacactacc ctgtcgccgg ccttgcccac tgcactgaac
     2881 tttacccagc cggacatatt ctgtccgaag tctatgacca gctcatcctt gggggttttg
     2941 aacatggata taggttttag aacctcccgc ttacgaatca taaccccatc ctgagggacc
     3001 accgtcgata tatccttgtc catcacccat acattagacc aatccgcatc atcaaacctg
     3061 ggcagatccc aaccctgttg ctctcgtctt gcatcgtagg tctcgccgtg atatagctct
     3121 gaatatatta cggggctttc cgaggccttc cagctatcat cggtgactac tatagcttca
     3181 cgtccgtcgg tatacctaac caacagctga agcaagagtc ccatttggct tccgtataca
     3241 ttccggcgac cgacccatcc cgccagatcc cccttatacc agcctatacc aagggatgcg
     3301 cctatggcgt tatcgcctgc ctttagaagc tctgtaacat cgtaggtttg gtattggagc
     3361 ctctccctat agtccgtcca gcccggtgtc aagagcccat cccctacttg ggcaccgttt
     3421 atgtagagtt catacattcc aagggaggtg gcatagaccc tggcaaattc aatctcgcca
     3481 tctaaattaa attctctcct caaaagtttt cctttggacg aagccccctc gtcctcccct
     3541 tccggactta taaatgccgc cttccactca gaggcatcca ggataccggt ttcaaagaac
     3601 gctgcatcgc tccagggact ttcattttta tggttatcgg acaccttcac cctataaaaa
     3661 tatctggcag aggattccaa tttaggccct gcatattcaa cgtggacgga ttctgacgag
     3721 tctattatgc ctgtgtccca aataggatct gtaaagtcct cgcccatact cacctgtagg
     3781 cggtagctct cctggacaac atttctattg tccgaatcca atatccagcc aaaccgggga
     3841 ttcgtctcac ccatccctat gggattgtcc aaatattcgc atctcagccc tatagttttt
     3901 agcaatatta ccactcctta tcctcgtaat catgatcgca acacatcaag gggacgcggt
     3961 tgcagatcgg gtatctgtcg gcaatagcta ttggacgcct attgcaactc ttaccaatag
     4021 cccattatcc ttagcgtcgg gtaagataat gagccggcgc aacaatttaa gaaaactttg
     4081 caaatcatat gccccatacc gtacaacaaa cttcattatc catatcaatt ttatcatata
     4141 tcaatctttt tattgtttat tagctggttt ctcatccaat gggtttttac caacccgtcc
     4201 ccctcctcca gcaaaatctc atatagtctt ttcctaagct ccgttactat gggcatatat
     4261 ttttcctgtc ccattagatt atagagctcg tcaggatcct tttcaaggtc atataactcg
     4321 tctatgtccg tggtattcca gatatacttc cagctattat ttcgtatcat gcgctgggta
     4381 tacaggccaa attgctgacc gttatacgtt gataccgcct cggttcgcca accctctgcc
     4441 ttttgtccct ttagcaggtt aagcatggat cttcccgaat aggaatccgg aatactttgt
     4501 cctaaaacct ccaatatggt aggtggaata tctaaaaagt gacatatgaa ttcgtccctt
     4561 acggaagacg gcgggattac cccgggccat cggataacca aggggacttt gaccacgtca
     4621 tcgtatagga tatagtgctt atctatcatc ctatggcccc cgcacatatc cccatggtct
     4681 gcggtatata tgactatggt gttctcggct tggcctaatt cttccaacct atcgagcact
     4741 ttgcctatgg catggtctat ctcggttatg gtggcataat aaagggctac cgtatcccgc
     4801 caatcctccc atgtatagtc ctctatatcc cagctgtaga gctgttgctt ttggatgtag
     4861 ggcttattat gaaagtcctc tttaaagctg gcccatggcg gtatgtcctc tgccttatac
     4921 atccgagcga agtcctcaga cggcctgcag ggaaggtgag gtccgggaaa gtccaggcga
     4981 atatgccacg gtgctccttc ttcggaataa ttattg
//
LOCUS       DASRNX010000087        10718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_387642,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000087 DASRNX010000000
VERSION     DASRNX010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387642"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..715
                     /locus_tag="VFD89_03405"
     CDS             134..715
                     /locus_tag="VFD89_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HZJ57269.1"
                     /translation="MEKIIPTREKAMELLKEYNQNDSLIKHALSVEAVMVHFARLFGE
                     DIEKWGIIGLIHDLDYERYPDEHCTKTKEILTERGWPEDYIHAVISHGWGICSDVEPT
                     EKMEKVLYTIDELTGLIAATALMRPSKSILDLTTKSVRKKWKQKSFAAGVDRGLIEKG
                     VQMLEMELGEVIEETIKGMQKAAEAIGLKGNLG"
     gene            874..1266
                     /locus_tag="VFD89_03410"
     CDS             874..1266
                     /locus_tag="VFD89_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57270.1"
                     /translation="MDREQREIKGIDSKDELKLANERRIDELLDISNRYVRTQRHLEE
                     HSDISDPDNLRHAHKIQEEREDRMENLKNIIAYGKHEEVDETENLERNLEFADGYLEN
                     NASRMDELTLDKTREKQAHRKEQLEFLD"
     gene            1393..2385
                     /locus_tag="VFD89_03415"
     CDS             1393..2385
                     /locus_tag="VFD89_03415"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017826060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HZJ57271.1"
                     /translation="MNQKIRIGITGYGNLGKGVEAAVRYNPDMELKAIFTRRDPVDIM
                     AETTRANPVHISEVESYIGKIDVMILCGGSATDLPQQGPYLAGMFNTVDSYDNHGRIP
                     EYFAKMDNVSKEASKVSMISVGWDPGIFSVNRLIQEAILPRGNTYTFWGRGVSQGHSD
                     AVRRVPGVQNAVQYTIPIEEALDRVRGGENPALSAGERHLRQCYVVAEKGADKGKIER
                     QIKTMPNYFEDYDTEVIFVDEEELRADHAGMPHGGIVIRSGETGHHYNHNQIVEFSLN
                     LGSNPEFTGSILTTYARAVHKMALEGQYGAKTAFDVPPAYLSIKSNEQLRKELL"
     gene            2773..3720
                     /gene="hflK"
                     /locus_tag="VFD89_03420"
     CDS             2773..3720
                     /gene="hflK"
                     /locus_tag="VFD89_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006837809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HZJ57272.1"
                     /translation="MYLKGIHKRGIIIGAIILVVIILLLSSFYTVDQTDYAVLMTFGR
                     PSAEIITPGLKYKFPYPIQDVKKLSKETFSLTLGYEDLNGEEIYDEVESKMITGDENI
                     ILADMEVQWRIVDPIAFLYNTEDPIEVLYVATSSSLRGVIGSALVDDVLTDGRTQITN
                     DIRDNLVKLVDRYGLGISIVNVNLQDVDLPTLEVSEAFKAVTDAREERVTKINNAEKY
                     KNEKMNEVMGERDALISDAEAVKTSRIEKAKGDVAKFNAVYSEYINSREVTKQRLVIE
                     TLQQILPKTKIYIVDDASGTVKYLPLETIEREAVQHDQQ"
     gene            3707..4591
                     /locus_tag="VFD89_03425"
     CDS             3707..4591
                     /locus_tag="VFD89_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HZJ57273.1"
                     /translation="MTSNKKIVFLTVTVIAIVILVAIVLSNLYIIHPNEYKVVKEFGR
                     IIEVADTPGLRMKVPFIQDVTTLPKQILLYDVPPAEINTLDKKRIMVDYYSMWQITDP
                     EKMLETLRTINGAEARLGDIIYSNIRTELGKLEYGEIINHTIKSRGDADIIVKDRVND
                     ILKANSNGIEVVDIQMKRADLPTSNEESVYKRMISERESKAQEYLSQGDAEATRIQAT
                     TNKDVSETLARADAKAKEIIAQGEKEAAKIYNESYGKDTEFFDLYTTLESYRTTINDE
                     TVIMFPADSPYIKYIFGK"
     gene            4809..6200
                     /gene="asnS"
                     /locus_tag="VFD89_03430"
     CDS             4809..6200
                     /gene="asnS"
                     /locus_tag="VFD89_03430"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HZJ57274.1"
                     /translation="MSMTLVRNIYGDPGSYKDQIVTLSGWVRTVRASKSFGFIELNDG
                     TFFKNIQVVYEESLENFKEISRFNVGSAIQVTGELVLTPGAKQPFEVKAQKVDLMGSS
                     TPDYPLQKKRHSLEFMREIAHLRPRSNTFSAVFRVRSLASYAIHKFFNDRDFVYVHTP
                     IITGSDAEGAGEMFRLTTLDLKDLPLHESGGVDYSHDFFGKEVSLTVSGQLEGEAYAH
                     AFRNIYTFGPTFRAENSNTARHAAEFWMLEPEIAFADLEDNMALAEEMLKYIISYVLE
                     HAPEEMGFFNRFIEKGLLDRLNMIVNSNFGRVTYSEAIDILKKSGVEFDYPVEWGCDL
                     QTEHERYLSETVFEKPVFVTDYPKDIKAFYMRLNDDGKTVAAMDLLVPGVGEIIGGSQ
                     REERLDVLESRIAEMGMDKEDYWWYLELRKYGGTKHAGYGLGFERAIMYMTGMGNIRD
                     VIPFPRTVRSAEF"
     gene            6496..7404
                     /locus_tag="VFD89_03435"
     CDS             6496..7404
                     /locus_tag="VFD89_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HZJ57275.1"
                     /translation="MGNNPVIEQVMVLFLIMIVGMVAAKRKIINDEVRKRLSELLLYI
                     TSPFLVVLSFNFRYSSDMLINAGIVFALSFGIHGLTAILGKYLFIKYPVDKQRVLRFI
                     IVFSNCGFMGFPVLEGLYGRMGVFYGSIYNVVFHIFVWTYGVMLFSADRESASIHKAF
                     LNPGIFSVAIGMIVFLFSLDLPKPIIQTLDMVGSMTAPLSMLIIGALLADTDLKKIFI
                     GWEVYYGSLLRLIIIPLITIAILKPIGIDRDLLRICVVLTAMPAAANTVIFAERYGAN
                     SLLASRCVALSTILSIITIPLIMLLT"
     misc_binding    7654..7882
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8013..>10718
                     /gene="ileS"
                     /locus_tag="VFD89_03440"
     CDS             8013..>10718
                     /gene="ileS"
                     /locus_tag="VFD89_03440"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZJ57276.1"
                     /translation="MELFRSLSNLPVNENEKRIAEKWEEIDILQRSIDSREGKESYLF
                     YEGPPTANGNPGIHHVISRTIKDTVCRYKTMTGYQVKRKAGWDTHGLPVEIEVEKQLG
                     LKNKQEIEEYGIDKFNKKCRESVFAYEKQWREMTRRMGYEIDLDNPYITLENDYIETV
                     WWILDKFFKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEIKTDTVYVKFKVKGADE
                     YFLVWTTTPWTLASNVALTVNPDETYLKIKAGEEVYYVEKNLADQVVGEDYQVIEEIK
                     GRDLEYIEYEQIMPFVYVPEGKKAFFVTLADYVTVEDGTGIVHTAPAFGEDDYNTGQR
                     YNLPVLQPVDEDGIFVETPWEGDFVMDADPAIIRWLAEEGKLFRKQRMEHNYPHCWRC
                     QTPLLYYAKPSWYIEMTRLKDKLIENNNSVEWYPDYVGEKRFGNWLENLNDWAISRSR
                     YWGTPLNIWRCDCGHDESIGSRKELVERAIEDIDEGIELHRPYVDDVHIKCSKCGGTM
                     TRVKDVIDCWFDSGSMPFAQHHYPFKNKENFQELFPADFICEGIDQTRGWFYSLLAIS
                     TFVMGVSPYKRVLVNDLILDKEGKKMSKSRGNTVDPFDLFEEYGADALRWYLLFVSPV
                     WTPTRFDIDGLKEVVSKFFVTIRNVYNFFTLYANTDKLNPRNFFVDYEARAELDRWIL
                     SKYNNLKKAIEEDMDAYDLTRAVRRIQDFANEDLSNWYIRRSRRRFWESELTEDKKAV
                     YSTTYEVLVGLSQMIAPFAPFISEEIYTNLTGELSVHLSDFPKVNLELIDERVEKRMD
                     LIRSLVTLGRSARESARIKVRQPLKEIFIDGKYKSLISDLIPLIKEELNIKEVVFAEN
                     LNEYMDFTIKPNFRVLGPVLGPKIRAFQKALSTLDTAVVAPMLDEG"
BASE COUNT         3145 a         2106 c         2788 g         2679 t
ORIGIN      
        1 gtcccaaccg ctatagtaag taattttggt ggtttggcat tgctttaacc cgttttgccg
       61 gcgtattatc tgtatatatt taaccggcat aacggacggc ttaaagcatt ctaaaggaaa
      121 aggagttttt gctatggaaa aaatcatacc gaccagggaa aaggcaatgg agctgttgaa
      181 ggaatacaac caaaacgaca gcctgataaa acacgctctt tcggtggaag cggtgatggt
      241 gcattttgcc aggctttttg gtgaagacat agaaaaatgg gggattatag gtctgatcca
      301 tgaccttgat tatgaaaggt atcctgacga acattgcaca aaaaccaaag agatattgac
      361 ggagaggggc tggcccgaag actacatcca cgcggtaata agccacggat ggggcatttg
      421 ctccgatgtg gagcctacag agaaaatgga gaaggtctta tacaccatag atgagctgac
      481 aggtctgatt gcagctacag cccttatgcg cccaagcaag agcatattgg atttgacgac
      541 aaaatctgtg agaaagaaat ggaaacagaa gagcttcgcg gcaggagtcg acaggggatt
      601 aatagaaaag ggcgtgcaga tgctggaaat ggagctcggt gaggttatcg aagagaccat
      661 aaaggggatg cagaaagccg ctgaggcgat tgggctaaag ggcaatttgg gttgaagatc
      721 ccccgaaaat tttgtgccag atattaggtg gtcctacaaa agtatccccc ggctcggtat
      781 atttagcatg tccttcatgg gatttttcgc ttaaatataa atcgaattat agggaaaaat
      841 aatatcgatt gtatgattga agggaggaat tctgtggacc gggaacagag ggagataaag
      901 ggaatagaca gtaaggatga actaaagctg gccaatgagc ggcggataga tgagttgtta
      961 gatattagca atcgctatgt acgcacccag aggcacctgg aagagcattc tgatatttcc
     1021 gaccctgata accttaggca cgcccataag attcaagaag aacgggaaga caggatggag
     1081 aacctaaaga atataatcgc ttacgggaag cacgaagaag tcgatgaaac ggagaatctg
     1141 gagaggaatt tagaatttgc cgacggatat cttgaaaata atgcttcgcg tatggacgaa
     1201 ttaacgctgg acaagaccag ggaaaaacaa gcccatagga aggaacagct ggaattcttg
     1261 gactaaatag ctcccgcccc atgggcgggg gtctattgtc ctaataatag ccgttacaca
     1321 tatatatccg actggtacaa tgcgttaatt cattttatga catcaaacgc caaaatttgg
     1381 ggggaaaaca atatgaatca aaagattaga attgggatca cgggctatgg caatctgggg
     1441 aagggcgtgg aggcagcggt gaggtataac ccggatatgg aactgaaagc gatttttaca
     1501 aggcgtgatc cagtggatat tatggcagaa accacccgtg ccaatccggt acatatctca
     1561 gaggtggaat cctacatagg aaagattgat gtaatgatac tctgcggcgg ttcggcaacg
     1621 gacctgcccc aacagggtcc atatcttgcc gggatgttca acaccgtgga cagctatgac
     1681 aaccacggga ggatcccgga atatttcgct aaaatggata atgtctccaa ggaggcatct
     1741 aaggtcagta tgatttcggt gggctgggat cctgggatct tttctgtgaa ccggttaatt
     1801 caagaggcca tcctgccaag gggcaacacc tatacctttt ggggcagggg agtaagccag
     1861 ggccattccg atgcggtaag gcgggtaccc ggggtacaga atgcagttca gtataccatt
     1921 cccatagaag aggcactgga tagggtcagg ggaggggaaa accccgcgct atccgctggg
     1981 gagagacacc tcagacaatg ctacgtggtg gccgagaaag gggcggataa ggggaaaata
     2041 gaacggcaga taaagaccat gcccaattat tttgaagact atgatacgga agtcatattt
     2101 gtagacgagg aagaactaag agccgaccat gcaggaatgc cccatggagg tatagtcatc
     2161 cgtagcgggg aaaccggcca tcattacaat cataaccaga tcgtcgagtt ttcattgaat
     2221 ttagggagca atcccgaatt taccggtagc atattgacga cttatgccag agcagtacac
     2281 aaaatggctt tggaaggcca atacggggct aagacagcct ttgatgtccc accggcctat
     2341 ctgtctataa aatccaacga acaattgaga aaagaattgt tatgatttga cctatcaata
     2401 tgccgattca catcagacta caaggctcgt atatgaaata atgagtatat aatatattgt
     2461 aaaaagtcat agctgtgata tgatagcatc atattcagcc cggaccccca gtttatttaa
     2521 atcgggagtc cggggaaagc ctatgtagaa cattggcgat ttgcgtaccg agcatcaaat
     2581 tgaatcttac actatatgtt gtgcgacaaa tatttttcat gggtatacgc gtgtcgcaaa
     2641 acatcattct tatttcatat gttacgaaaa atacattgtt ttggcacaaa attagatgat
     2701 ttagctgatt ttgtaaagaa agatcttctt cgcgacatat aggttcaatt catatatgaa
     2761 ggggtggttt gtgtgtattt aaaaggtatc cataaaaggg gtataattat aggtgcaatc
     2821 atattggtgg ttatcattct attattatcc tccttttata ccgtagatca gactgactat
     2881 gcagtgctga tgacgtttgg aagacccagc gccgagatta taacaccggg actcaagtat
     2941 aaattccctt acccaattca agacgtaaaa aagctgtcaa aggaaacatt ctcattgacc
     3001 ttaggttatg aggatctaaa cggcgaagaa atctatgacg aagtggaatc caagatgata
     3061 acaggggacg aaaatatcat cttagcggat atggaagtac agtggagaat agtggacccc
     3121 atcgccttcc tttataatac ggaagatccc atagaggttc tctatgttgc cacctcatcg
     3181 tccctgaggg gcgttatagg cagcgctttg gtagatgatg tattgaccga cggtcgtact
     3241 cagataacca acgacataag ggataatctt gtaaaactgg tggaccgata tgggcttgga
     3301 atatccatag tgaatgtgaa cctccaggat gtggatcttc ctaccctcga ggtaagcgag
     3361 gcctttaagg ccgtaaccga tgccagggag gagagggtca ccaagataaa caatgcggaa
     3421 aagtataaga acgagaagat gaatgaggtt atgggcgagc gggatgcctt aataagcgat
     3481 gccgaggccg taaagacctc ccgtatagaa aaggccaagg gggatgtggc aaagtttaat
     3541 gccgtttatt cagaatatat caatagccga gaggtaacaa aacagagatt ggttattgaa
     3601 accctgcagc agatcctgcc aaagacaaag atctatatag tcgatgatgc ttcaggcacg
     3661 gtaaaatatc ttccgttgga gaccatagaa agggaggcgg ttcaacatga ccagcaataa
     3721 aaaaatagtt tttctgaccg ttaccgtaat agcaatcgtt atcctggttg ccatcgtcct
     3781 cagtaacctg tatattattc atcctaatga atataaggta gtaaaggaat ttggtaggat
     3841 aattgaggtg gcagacaccc cggggctccg tatgaaggtt ccctttatcc aggatgtcac
     3901 taccctcccc aaacagattc tgttatacga tgttccgccg gcggagatca acactttaga
     3961 taaaaagcga atcatggtgg actactatag catgtggcag attaccgatc ccgaaaagat
     4021 gctggagacc ttaaggacca taaatggggc ggaggcaagg cttggggaca tcatatattc
     4081 taatatcagg acggaacttg gaaaattgga gtatggcgag atcatcaacc ataccatcaa
     4141 atccaggggt gatgcggata tcatagtgaa ggatagggta aatgacattc taaaggctaa
     4201 ttccaacggt atagaagtgg tggatataca gatgaaacgg gcagacctgc cgaccagcaa
     4261 cgaggaatcc gtctacaaaa ggatgatctc cgaaagggag agtaaagccc aagaatacct
     4321 gtcccaaggc gatgcggaag cgaccaggat acaggccact accaataagg atgtgtcgga
     4381 gaccctggcc agagccgatg ccaaggcaaa ggagataatc gcacaggggg agaaggaagc
     4441 cgctaaaatt tacaatgaat cctacgggaa ggatacggag ttttttgatc tgtacactac
     4501 tttagaatcc tataggacaa ctataaatga cgaaaccgtt atcatgtttc ctgcggattc
     4561 gccatatata aagtatatat tcgggaaata gactgttttg cctgcggggt agctggcatc
     4621 ataatttggc gcatgatttt gcccggaggt tttgtcctcg gacgattgtt ttgcatgtaa
     4681 ccaataatcg aggatgcgca tcttattttg cgtactctgt gaattgggct gatataggcg
     4741 gagcagccgg ggattgatgc gcccggatca tatacttgca gatgtatcat tattagggag
     4801 gaagtcgaat gtcaatgaca ttggtaagga atatctatgg ggatccaggg agttataagg
     4861 accaaatcgt tactttatcc ggctgggtca ggacggtaag agcctctaag agctttggct
     4921 ttattgaact caacgatggt acctttttta aaaacattca ggtcgtatat gaagaaagct
     4981 tggaaaactt taaagagata tccagattta acgtaggttc cgctatccaa gtgacgggcg
     5041 agctggtctt aacccccggg gccaagcagc cctttgaagt gaaggcacag aaggttgatt
     5101 taatgggaag ttccacgccc gattatcccc ttcagaaaaa gcgacattct ctggaattta
     5161 tgagggagat tgcgcaccta cgccccagga gcaatacctt ttccgctgta tttagggtgc
     5221 gatccttggc atcatatgct atccataagt tctttaatga cagggatttt gtctatgtcc
     5281 acacgcccat cattaccggc agcgatgcag agggtgcagg cgagatgttc cggctgacaa
     5341 cccttgatct taaagacctg ccccttcatg aatcaggcgg ggtggattac tcccatgatt
     5401 tttttgggaa ggaagtcagc ctgacggtaa gcgggcagct ggaaggggag gcctatgccc
     5461 acgccttcag aaatatctac accttcgggc ctacgtttag agcggagaat tccaatacgg
     5521 ctcggcatgc cgcagaattt tggatgctgg agccggagat tgcctttgct gaccttgaag
     5581 ataatatggc cctggcagag gagatgctaa agtatataat ttcttatgta ttagagcatg
     5641 cccctgagga gatggggttc tttaataggt ttatcgaaaa gggattgctg gacaggctga
     5701 acatgatagt aaattcaaac tttggtaggg ttacctacag cgaggccata gatatattaa
     5761 agaagtccgg ggtagagttt gactatcccg ttgagtgggg ttgtgacctt cagacggaac
     5821 atgagagata tctatcggag acggtttttg aaaagccggt cttcgttacg gattatccca
     5881 aggatataaa ggcattttac atgcggctca acgacgacgg aaagaccgtg gctgctatgg
     5941 atctgcttgt gcccggggtt ggcgaaatca tcggcggcag ccaaagggaa gaacgcttgg
     6001 atgtcttgga aagtcgcata gccgaaatgg gcatggataa ggaagactat tggtggtatc
     6061 tggagcttag aaagtatggc ggaacaaagc acgccggcta tggtctgggg tttgagcggg
     6121 ctatcatgta catgaccggc atgggcaaca tccgagatgt catccctttc cccaggaccg
     6181 taagatccgc cgaattttag tggatgaacg aacctataat tcacactata acggcaatca
     6241 aacctgtggt ataatggcta ttggatatat ttttcgacat aaactaacgc attgtgctgt
     6301 ctcattacct tgccgcatga caaaggcagt gagatagtga taagcggacg attttatttg
     6361 atgtggcccc gaatcacaat atcggaggca gcgtgtaaca gccggtgccg gcatatattc
     6421 agctggcgcg actaatgaaa ttaaggtaaa acaatggccc attatgatta aggggcctaa
     6481 tttgggggat aaaaattggg gaacaatccg gtgattgaac aggtaatggt actattttta
     6541 ataatgatag tgggtatggt agcagctaag cgcaagataa tcaacgatga ggtcagaaaa
     6601 agattatcgg aattgctcct atacatcact tcaccctttt tggtggtgct gtcctttaat
     6661 ttcaggtatt catcggacat gcttatcaat gccggtatag tctttgccct gtcctttggg
     6721 atacacggtt taaccgctat cttaggcaaa tacctcttca tcaaataccc tgtcgacaaa
     6781 caaagggttt taaggttcat aattgtattt tccaattgcg gatttatggg gttcccggtt
     6841 ctggaagggc tctatggaag gatgggggtc ttttatgggt ctatttataa tgttgtcttt
     6901 catatcttcg tttggaccta tggggtgatg ctctttagcg ccgacaggga atcggcttcc
     6961 atacacaaag cctttttaaa ccccgggatt ttttcggttg caataggcat gatcgttttc
     7021 ttattttcat tggatctgcc aaagcccatc atccagacat tggatatggt ggggagcatg
     7081 acggcgccgc tgtccatgct tatcataggg gccctgttgg cggatacgga tttgaagaaa
     7141 atatttatcg gatgggaagt gtattatggc tccttattga ggcttataat aattcccttg
     7201 ataaccatcg cgatcctaaa gcccataggc atagacaggg atcttcttag gatatgtgtc
     7261 gtattgactg ccatgcccgc cgctgctaac accgtgatct ttgccgaaag atatggggcc
     7321 aattccctgc tggcctcccg ctgtgtggcc ctgtccacta tcctgtccat tattaccata
     7381 ccccttataa tgttgttgac ttgatagggt attatgcaaa ggtataattg ggtaaatagc
     7441 tactagaata aattttaaaa aaggttaaac ctgcggtatt gtgacacaat atatcgaata
     7501 atgtatttta tgggaccccc gaggttttgg taaaaatcgg ccttgacaaa accatggttt
     7561 ttgcggtaat atgaagcata atgggcgttt atgaaaactt taacgattgt gctatagttg
     7621 tcattacaat cggcttcgac attttaatag attacaaaga ctatgaacgg gacagtagta
     7681 aggttatttt gttttagaga gacggccata cggtgaaagg ccgccacaaa aatctttacg
     7741 aagatcgccc gggagttcga tacctgaagg ccttccgacg gatggcatat agggtatcgc
     7801 gcgagatgcg ttatacaaaa acgagcgata ggcagattta tgcctataag ctgggtggta
     7861 acacggcaaa gtcgtccctg agtttttgac tcggggattt ttgtttatta cagataaacg
     7921 ataggtcaac gggatttagt tgcagatgat gacttttatg gttgaagttg tttatcaata
     7981 tagagattga ggcttagaaa ggaagttaag cgatggaatt atttagatcc ctatccaatc
     8041 ttcccgtcaa tgagaatgaa aagaggattg cggagaagtg ggaggaaatc gatatactac
     8101 agaggagtat agacagccgg gagggaaagg aatcatactt attctatgaa ggtcctccca
     8161 cggctaacgg gaaccccggc atccaccatg tcatttccag gactatcaag gatacggtat
     8221 gcagatacaa gaccatgacc ggatatcagg ttaagagaaa ggccggatgg gatacccatg
     8281 gactgcccgt agagattgag gtagaaaagc agttggggct aaagaataag caggaaatag
     8341 aggaatacgg tatagacaag ttcaataaaa aatgtcggga atccgtattt gcctatgaaa
     8401 agcaatggcg tgagatgacc aggaggatgg gctatgagat cgacctggat aatccctaca
     8461 tcaccttgga aaacgactat atagaaaccg tctggtggat actggataag ttctttaaag
     8521 agggatatat ctatgaagga cataagatcc tgccctattg cccccgctgt ggtacggggc
     8581 tggcatccca tgaggttgcc cagggctaca aggaaataaa gaccgatacg gtttatgtta
     8641 agtttaaggt aaagggtgca gacgaatact tcctggtatg gaccactacc ccctggaccc
     8701 tggcatccaa cgttgctctg accgtgaatc ccgatgagac ctacctgaag atcaaggcag
     8761 gtgaagaggt ctattatgta gaaaagaacc ttgccgatca ggtggtggga gaggattatc
     8821 aggttataga ggagataaag ggcagggatt tggagtatat tgaatatgaa cagataatgc
     8881 cctttgtcta cgttccggag gggaagaagg cctttttcgt aactctggca gattacgtaa
     8941 ccgtggagga cggtacggga atagtccata ccgcaccggc cttcggtgag gatgactaca
     9001 ataccggtca aaggtataac ctgccggtgc tccagcccgt ggacgaggac ggtatttttg
     9061 tagagacccc ttgggagggg gacttcgtca tggatgcgga ccctgctatt atcaggtggc
     9121 tggcggagga aggcaagcta tttagaaaac agaggatgga gcataattac cctcactgct
     9181 ggagatgtca gactccgctt ctgtactatg ccaagcccag ctggtatatt gaaatgacca
     9241 ggctcaagga taagctcatc gaaaataata atagcgtaga atggtacccg gattatgtgg
     9301 gagagaagag gttcgggaat tggctggaaa acctcaacga ttgggcaata tccaggagca
     9361 ggtattgggg aaccccatta aacatctgga ggtgcgattg cggccacgat gagagcatcg
     9421 gctcccgaaa ggaactggta gagcgggcca tagaggatat agacgagggc attgaacttc
     9481 ataggcccta tgtagacgat gttcatataa agtgttcaaa gtgcgggggc accatgacca
     9541 gggtcaagga tgtcatcgac tgctggtttg acagcgggtc catgcccttt gcccagcatc
     9601 attatccatt taagaataag gagaactttc aggagctctt ccctgcagac tttatatgcg
     9661 agggaataga ccagaccaga ggctggtttt attcgctcct tgccatctcg accttcgtta
     9721 tgggggtatc cccctacaag agggtgctgg ttaacgatct gattttggat aaggaaggca
     9781 agaagatgtc taaatccagg ggaaataccg tggatccctt cgatctattc gaagaatacg
     9841 gtgccgatgc cctgcgctgg tatctcctgt ttgtttcacc tgtatggacc cctacccgct
     9901 ttgatataga tgggcttaag gaagtggtca gcaagttctt tgtaaccata aggaacgtat
     9961 acaacttctt taccctctac gccaataccg ataagctaaa tcccaggaac tttttcgtgg
    10021 attacgaggc cagagccgaa ctggataggt ggatcctgtc caagtacaac aaccttaaaa
    10081 aggctataga agaggatatg gatgcctatg acctcaccag ggctgtgagg aggatacagg
    10141 attttgccaa cgaggacctg tccaactggt atatccgccg atccaggaga agattctggg
    10201 aatccgaatt aacggaggat aagaaggcgg tatatagcac cacctatgag gtactggtag
    10261 ggctttctca gatgatagcc ccctttgctc cctttatatc cgaggagata tacaccaatt
    10321 taaccgggga actttcggtt catcttagcg attttccaaa ggtgaattta gagctcattg
    10381 acgagagggt agagaagcga atggacctta taaggagtct ggttaccctt ggacgttctg
    10441 ccagagaatc tgcccggatc aaggtacgcc aacccctaaa agagatcttc atagacggta
    10501 aatacaaatc tttaatttcg gacttgatac ctttgatcaa ggaagagctg aacatcaaag
    10561 aggtagtgtt tgccgaaaac ctaaacgaat atatggactt taccataaag cccaacttca
    10621 gggtcctagg accggtcctt gggcctaaga tcagagcgtt tcaaaaggcc ttgtccacgc
    10681 tggatactgc agttgttgct ccaatgctgg atgagggt
//
LOCUS       DASRNX010000088        15719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_387943,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000088 DASRNX010000000
VERSION     DASRNX010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15719
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387943"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(340..618)
                     /locus_tag="VFD89_03445"
     CDS             complement(340..618)
                     /locus_tag="VFD89_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HZJ57277.1"
                     /translation="MYQDKTLVCKDCGQDFTFTAGEQEFYAEKGFQNEPARCRDCRDA
                     RKANRRSNSGRREMHDAVCAECGAHTQVPFVPRNDRPIYCSDCYQNHR"
     gene            complement(1117..1452)
                     /locus_tag="VFD89_03450"
     CDS             complement(1117..1452)
                     /locus_tag="VFD89_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZJ57278.1"
                     /translation="MPDYKGSLKDINKSMMELGKLLPDEMNGFMNLHNAALAKGAMDP
                     KTKELIALGISICIRCEPCIVSHVDSLIKMGVTKEEIKETVAVAIFMGGGPSVAYGGK
                     VLEAFEQLS"
     gene            complement(1541..1807)
                     /locus_tag="VFD89_03455"
     CDS             complement(1541..1807)
                     /locus_tag="VFD89_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HZJ57279.1"
                     /translation="MEQRDIKKAVVLSYDENDQAPAIVAKGEGYVAQRIIDIAASGGI
                     PLVRSPEQVDALSRVEIGEYIPPELYQAVAEIYAFLMRVDVDIS"
     gene            complement(1821..2717)
                     /locus_tag="VFD89_03460"
     CDS             complement(1821..2717)
                     /locus_tag="VFD89_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57280.1"
                     /translation="MDIYSRIMTRLYTLAKTDTVGIPDPKIGDGMEEVARQDPPLGAG
                     TESGDHPMKSILGKLGLKDTPENRALIMLIPLYGLSFNEESARFILGKGHWSSFLYEH
                     LLERILEYGNIEGARAVEESFPDIHTLPEFLKNLRSNMNKIWGHIIEQSESLAEGIKS
                     ESLKTLLGDIYSNILLQLDNSHPFYNVQIPVRIRDRIYSCQVWCGRERDVIYLEFMLP
                     NLNKIGCLMEEWRGPLKVALYTEGKYHKVIKNGFPHLKSVLNGLGYRCKSLEVYDIAR
                     FSGLADFLSDFKEVYRMIDLSI"
     gene            3181..3492
                     /locus_tag="VFD89_03465"
     CDS             3181..3492
                     /locus_tag="VFD89_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57281.1"
                     /translation="MNINRPLECPECSGTHLEIKKEATYLYTYKVDTPLTEGWSKEKE
                     ALPFLFDNRELVGTNDYIQCAECGAKYPCDLEKGDIKIHFTIMQKAIRSDFQEYPEYL
                     G"
     gene            complement(3499..8376)
                     /locus_tag="VFD89_03470"
     CDS             complement(3499..8376)
                     /locus_tag="VFD89_03470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HZJ57282.1"
                     /translation="MRIVTFADIEVDINSGRILDIGSIKSNGDTFHCGSVDEFAKFLC
                     GSQYICGHNILNHDLKYIEDVITQKEFDDLRYIDTLYLSPLLFPARPYHALLKNDKLQ
                     SDDVNNPLNDSIKARDLFFDEVTAYNHTDKGFRDILYHLLKDEKEFSGFFHFIKCRAN
                     GSDIENRDDIGDKTNPGNKIDIKGAIKKKFHPHICENADLKGMISAHPIELAYCLALI
                     NANNRYSITPPWVLKSFPRVENVMYMLRGRPCLEGCTYCHKALDIHNALKTYFGYDSY
                     REYGGEPLQEKAVKAAVDNKSLLAVFPTGGGKSIAFQIPALISGDSTKGLTVVISPLQ
                     SLMKDQVDNLEEVGITEAVTINGLLDPIERAKSFERVEKGSASILYISPESLRSRTIE
                     RLLLGRNIVRFVIDEAHCFSAWGQDFRVDYMYIGDFIKSIQEKKNIDGGIPVSCFTAT
                     AKQNVIDDIQKYFKDKLSLELNLYTAGTSRDNLRYMVFNKEGEEEKYNAVRDLIEGKK
                     CPTIIYVSRTRRASMLAQRLSQDGYPAKPYHGKMDKGEKTQNQDAFIKGEVDIMVATS
                     AFGMGVDKKDVGMVIHYEISDSLESYIQEAGRAGRDENMSADCYVLFNDEDLSKHFIL
                     LNQTKLSIKEIQQVWKAIKDVTGFRSKVSQSALEIARKAGWDDSVVDIETRVTAAIAA
                     LEDAGYLKRGQNAPRIFANSILTKNAREAIERIQTSERFMEDQKVSAVRIIRRLFSSK
                     SSKHQGDEAESRVDYISDHLGLPKGEVIQIINLLREEGILADAKDLTAFIRGRQGENH
                     SRKILRSYWQIERFLLSCVEDGEQTLHIKDLNQRAEESGCEGVTPGKIKTVLNFWAIK
                     NWIKKQTHGYSRNHIAIMPLHERNDLEDRLAKRYELSDFILGYLYEKSKTEPMGDPNG
                     QEDVLVEFSVLGIKEGFENRQTLIKAQVSSKDIEDALYYLSKIDALKIEGGFMVIYKG
                     LTIERIERDNRKRYKVQDYEKLKQFYDSKVQQIHIVGEYARKMIHDYKSALEFVDDYF
                     QLNYSSFLRKYFKGNRQKEITKNITPAKFRQLFGDLSPTQLKIINDSESKYIVVAAGP
                     GSGKTRILVHKLASLLLMEDVKHEQLLMVTFSRAAATEFKKRLLGLIGNAANFVEIKT
                     FHSYCFDLLGRVGSLEMSGQVIKEATDRIRNGDVEPGRITKTVLVIDEAQDMDADEFS
                     LIKALMEYNDNMRVIAVGDDDQNIFEFRGSSSKYLWELANKKGAAVYELVENYRSRAN
                     IVDFSNQFVQKISHRMKTTQIIPVKDYNGDIKIVRHRSRNLVTPLVNDLISGEITGTV
                     CVLTKTNEEAAQVTGLLLMNGIKARLIQSNDDFSLYNVLEVRYFLDHINSFGNIYTIS
                     DDLWEDAKAHLAERYSQSTNYGLCMNIIRDFELTNTGNKYISDLEVFIRESRMEDFLD
                     ESMETILVSTIHKAKGREFDNVFLVLDRFACERDESRRQLYVAMTRAKCNLTVHYNGE
                     YLDFIRAADLNVVDDLRIYTSPPQLAVQLGFKDVWLDFFQWRQHLISDLSSGDTLIVD
                     RDCCCNDNGQAVIRFSKGFVKRLETMKKYGYVPRTGRARFIVYWKKENTEREIEVILP
                     EIHFERIEGVQEANL"
     gene            8668..10245
                     /locus_tag="VFD89_03475"
     CDS             8668..10245
                     /locus_tag="VFD89_03475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HZJ57283.1"
                     /translation="MDFKFLIKKDKAYITSLSIFLVLMWLLSGCYSGEGADILNPVDE
                     SGPPYSGAVINPPTHHCESVDIDMEKEPEVVTVTITATGDCTLGINSKMVYPESFDAY
                     YDDYGPSYFFQNVRDILGEDDFTIVNLEGPLTTSNDEQTKIHNHKGKPEYVDILREGS
                     VEAVSLSNNHTFDYGISGYEDTVNALDQAGITWAGDDSYGLFEAKGVKIGFVSVDEHY
                     NGPLVEVRLKAGVEELRRQAADIAIACIHWGRAEDENTSRVDDYQVELGKKCIDWGYD
                     LIIGNHPHALQGIDRYRGKYITYCLGNFCYGGNKNLKDGDSGIFRQVFTLVDGELQTD
                     DNVQFIPCSSSSVADVNDYRPTIASGAEGHRIITKMNAYSAQYGVVFDLGGCITTKSL
                     SDGDRSDAYYSIFLESIREDALGDDIEYVAIDISSLDLSDSSYLMGLFEIWAKESDVK
                     VFLGTKEQIVEAGYHNYAGAGTGRVFIFSSPKWSNLQVTCSLSRLVTMLNYMRDDYTA
                     AYANEDWVAQKGGYIQK"
     gene            complement(10279..11154)
                     /locus_tag="VFD89_03480"
     CDS             complement(10279..11154)
                     /locus_tag="VFD89_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZJ57284.1"
                     /translation="MNYVMVIACIFIFMFQTISFKEFNKSHMKNIASYFLFNFIYFGM
                     VVLILLALGGFKGLKPYTLWLGISFGTLFVGTILCYMKAMGMGPLSFTALFFAFGLVV
                     PILYGLFFWNESISPLQLLGLLLLLITFYIGNESTDEDGEKKWGGKWLLFCLIAFVGN
                     GGLMVISKGHQVTMEGQETIEFLVIGFGTAAVISSLLFMWNFFRNKQDVSHFKSWSLA
                     WVSIVAGVSTAIGNWLALILSTRIPSVVQFPSINGGTVFLSTLASSLIFKEKLGKKAI
                     IGLIIGLLALVLLSL"
     gene            complement(11384..12295)
                     /locus_tag="VFD89_03485"
     CDS             complement(11384..12295)
                     /locus_tag="VFD89_03485"
                     /inference="COORDINATES: protein motif:HMM:NF036726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HZJ57285.1"
                     /translation="MKEITEYLSSFMEKIDFPKEAIDALLDVHDSLASNEAHNKGMDS
                     IIHRYYYDAGHKEIDEILNELTHLGQKMDINPYTMHLMFLLYCSRILKEKYKAAGVSL
                     DIFWNSMLDLGYKLRECRDIHGVWGTFVAGWLSDYFVMERFGLGRMQYEGISFSRDRY
                     IKNGYAVEKGDRVYNMHIPSAGPLTREKRMDSYKRAYDFFKDELNGGPIVLVCHSWLL
                     YPDNERFYPKGSNIIDFMHDFEIVYSVDEDNFSEAWRVFGRDSDKDIDKWPTDTSLQR
                     AYVDRLKAGNKTGAGFGIIIFDGEKIL"
     gene            12950..14206
                     /locus_tag="VFD89_03490"
     CDS             12950..14206
                     /locus_tag="VFD89_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZJ57286.1"
                     /translation="MGSKKRILDAINHRLPDRIPVDFGGSAVTGMHVTCVAALRDFYG
                     LEKRPVKINEPYQMLGLIEDDLQQIIGIDMVPLGQKNNMFGFPNDNWKEWRLRNGLEV
                     LVPGDFNITEDEAGNTYIYPEGDLTAPPSGKMPKGGYYFDSIIRQPEIIEEELDPNDN
                     LEEFKPLNEDDISYLVSEAKRTAKTGLGVLANFGGTALGDIALVPGPFMKHPKGIRDV
                     EEWYVSTVIRKDYIHKVFDKQIDIALQSLKLINERIGDLVDIAYICGTDFGTQTSTFC
                     SADTYRELYMPYYKRVNDWIHDNTDWKTFKHCCGAIEPFLSLFIESGFDIINPVQCSA
                     AGMKPEQLKKKYGKDIVFWGGGVDTQWTLPFGTPEEVRKQVLNRCEVFSKDGGFVFGA
                     IHNVQANTPVENIAAMIDAVHEFNSI"
     gene            14484..14750
                     /locus_tag="VFD89_03495"
     CDS             14484..14750
                     /locus_tag="VFD89_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57287.1"
                     /translation="MTNRTPLKKELLIGEAPLLRIIEIFDIKKIVAVGNKADESLTRL
                     GIDHEKVRHPAQGGKNEFVRGIRAIKKGLMSDNPMSRHTRFVTT"
     gene            complement(14740..15447)
                     /locus_tag="VFD89_03500"
     CDS             complement(14740..15447)
                     /locus_tag="VFD89_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HZJ57288.1"
                     /translation="MGIGTLVNTGAIILGGLLGITLKNAFSERIQNTLIKAIGICVLF
                     IGIGGALEEMLSYEGGKLVSGGSMMIIFSFVLGSLLGEGVNLERRIEQFGAWLRAKTR
                     NEKDTKFMDGFVSTSLIVCVGAMAVVGAIQDGLSGDYSLLMAKSVLDFIIVMIMAASI
                     GKGCIFAAIPVALLQGTITILAGFIEPLMTVRAMSNLSLTASMLIFCVGVNLVWDKKI
                     RVANMLPTILFALIFRW"
BASE COUNT         4326 a         3639 c         3196 g         4558 t
ORIGIN      
        1 gtatttatgc atacggttaa atgtttatta ctatatggaa aaatgctccg caaattatta
       61 cattatttcc cacgttttac caccccatta atctacacct gtggatatgt ggatgtctta
      121 cttgtggaca ttgtggataa ccctgtgaat aactcatgtt ttgggcattt ccccatgtgg
      181 ataactctgt ttattattgt cgggtccaaa aaacagaata ccttatgggc caaaattcga
      241 caattcgtat acccactaaa aaaccccgaa gcatgcttcg gggttttgtg accaattcaa
      301 tagtttaatg aggatgaaga acttgttcag cctatgggct tacctgtggt tctggtagca
      361 gtcgctgcaa tagattggtc tgtcatttct gggaacaaag gggacctggg tatgggcgcc
      421 gcattctgca catacagcat catgcatttc ccttctgcca ctgttgcttc tccgattagc
      481 ttttctagcg tcacggcagt ccctgcagcg agccggctcg ttctggaagc ctttttctgc
      541 ataaaattcc tgttcccctg cggtaaaggt aaagtcttgt ccgcagtctt tgcacactaa
      601 ggttttgtct tggtacatgg acataatctc ctcgctataa gtgaatttcc tagtttaaac
      661 ccagctgacc tggtctttgc ccccacactc gctgaccgga aggttcgctc aaggtgcatg
      721 cctcccacta ctacccctct ctatgtcaaa gcaaggtatt taacctgctt ggccataggt
      781 cagacttaca tctaagccgc accatgctca ccagtctttg accggattac gtggatcgtt
      841 ttgcctgcga ctggcatcga ttttcaacca cagacccaag ttcaaacata ggtatattat
      901 atatcccgcc atagcaaaaa gcaaccattt tattgaaatt atttattttt tgtgctcgtg
      961 ttgcccaata tgataaggtg catacccatt ccatgaccga ttcagagact atcccaattg
     1021 tccgccttga tcatagataa acaactccaa ctataagatt tatcatcttc atatagatca
     1081 atggacctat tgtttatcta taattggtgc cgatgatcag ctcaattgct cgaatgcctc
     1141 caatacttta ccaccataag cgaccgatgg accgcccccc atgaatatgg ccacggctac
     1201 ggtttcctta atctcctctt ttgttacccc catctttatc aggctgtcaa cgtggcttac
     1261 gatacagggt tcgcatctga tgcaaatgga aatccccaaa gctataagct ccttggtctt
     1321 aggatccatg gcccccttag ctaaggccgc attgtgaaga ttcataaaac cattcatctc
     1381 gtcaggcaat agcttgccca attccatcat acttttgtta atgtctttta aactcccctt
     1441 ataatcaggc atgattttgg ctcccttctt aaaatattta atctatctac gctaaaaacc
     1501 cctgatcatt atacagggta cgcacctaaa ctaaaattca ttaggatata tccacgtcga
     1561 ccctcattaa aaaagcatat atttctgcca cagcctgata gagctccggg gggatatatt
     1621 caccgatctc tacccgggat agggcatcaa cctgctccgg tgatcttacc aggggaatcc
     1681 ccccagatgc agcgatgtct ataatccttt gtgctacata gccttcaccc ttagctacta
     1741 ttgcaggagc ctgatcattt tcgtcataac ttaaaaccac agcctttttt atgtcccttt
     1801 gctccaagat tgccctccta tcatatactc aaatcaatca tcctgtatac ttccttgaaa
     1861 tctgaaagaa aatccgccaa tccgctgaac cgggcgatat cataaacctc cagggattta
     1921 cacctatatc ccagtccgtt aagcaccgat tttaagtggg gaaacccatt tttaataacc
     1981 ttgtgatatt tcccctcagt atataaggcg actttaaggg gacccctcca ttcttccatt
     2041 aagcagccga tcttgtttag gtttggtagc ataaattcta aatagatgac atccctttcc
     2101 ctgccgcacc agacctggca ggaataaatt ctgtccctga ttctaaccgg aatctgaaca
     2161 ttataaaagg gatgactatt gtccagttga aggagaatat tgctatatat atcaccgagt
     2221 aacgtcttca gactctcact tttaatgcct tctgccaggg actccgattg ctcaattata
     2281 tggccccaaa ttttattcat gttggatcga aggtttttga gaaattcggg gagggtgtga
     2341 atatcgggaa aggattcctc taccgccctt gccccctcta tgttgccgta ctctaaaatt
     2401 ctttctaata gatgctcata cagaaaggat gaccaatgcc cttttcccag gataaatctg
     2461 gcgctttcct cgttgaatga aagaccatat aggggtatta gcattattaa ggcacggttt
     2521 tccggcgtgt cctttaaccc taatttcccc aatatgcttt tcatcggatg atcccctgat
     2581 tctgtacctg cacccaaagg cgggtcttgg cgcgcaactt cctccatccc atccccaatc
     2641 tttgggtccg ggatgccgac ggtatccgtt ttggccaatg tatacagcct tgtcataatc
     2701 ctgctatata tatccatatc acaaacccat tctagttttc ctgtatattg tctgataccc
     2761 cagactatat acagataatc aatagattat ctaaatttat cggtataata attgcgaaaa
     2821 ttctgtgcaa tttcacgggg ctaaacttta tagttgaaat tgcttatcta caggtgctct
     2881 atcttgatca atataggaaa atacatctat cccacaactt aactataagt tgcatcttga
     2941 atataccacg ttttaccgat tggaccaagt acccttttgg ttatgatacg gaaaacctgt
     3001 ccgcgagaaa ctgtttaatt ccgtttttca ggaaagtccc gacagggatg atgctataga
     3061 aaaggaccat gcatacccta tcaattgttg ggggaaaata ctgactatag tggtgcgatg
     3121 atgattcagt tatgatatcc gtttcattaa atgaatttaa gcggagggaa aataaagacc
     3181 ttgaatatta atagaccatt ggaatgtccg gagtgtagcg gaacccattt agaaattaaa
     3241 aaggaagcaa cctatctata cacctacaag gtcgatactc cgctaacaga ggggtggagc
     3301 aaagaaaagg aggcattacc attcctattc gataacaggg agcttgtcgg tactaatgac
     3361 tacatccagt gcgcagaatg cggcgctaaa tacccctgtg atttggaaaa gggtgatatc
     3421 aagatccatt ttaccatcat gcaaaaggca atcagatccg attttcaaga ataccccgaa
     3481 tatttgggat aaaagtggct atagattggc ctcctgtacg ccctcaattc tttcaaaatg
     3541 gatctcaggc agaataacct ctatctcccg ctcggtgttt tcttttttcc aatagactat
     3601 aaatcttgct cggccggtcc ttgggacata gccatacttc ttcatagttt caagcctttt
     3661 aacaaaaccc ttggagaacc ttatgactgc ctgcccgtta tcgttacagc aacaatccct
     3721 gtcaactata agtgtatctc cgctgcttag atcggatatc aggtgttgcc tccattgaaa
     3781 gaaatccagc catacgtcct taaaccctaa ctgcaccgcc aattggggag gcgatgtgta
     3841 gatcctaaga tcgtcaacga cattaaggtc cgccgccctt ataaaatcaa gatattcccc
     3901 gttataatgg acggttaagt tgcattttgc cctggtcatg gcaacgtaga gctggcgtct
     3961 ggactcatcc ctttcacagg cgaatcggtc cagtaccaaa aaaacattat caaattccct
     4021 tcccttggct ttatggatgg tagaaactaa tattgtctcc atgctttcat cgagaaaatc
     4081 ctccatcctt gattcacgga tgaagacctc caaatccgat atatacttgt tcccggtgtt
     4141 ggtgagttca aaatccctga ttatattcat acacagacca tagtttgtgc tttggctgta
     4201 cctttcggcc aaatgggctt tggcatcctc ccacaaatca tcgctgatgg tatagatatt
     4261 tccgaatgaa tttatgtgat ctaagaagta gcgcacttcc aaaacgttat aaaggctgaa
     4321 atcatcgttt gattggatta gcctcgcctt aattccattc attaaaagca gcccggtgac
     4381 ctgggcggcc tcttcattgg tctttgtcag aacacagact gttcccgtaa tttccccgga
     4441 tattaggtca ttaaccaacg gtgtaaccag attccggctt ctgtggcgca ctatttttat
     4501 atcgccgtta taatccttga cgggaattat ctgagttgtt ttcatgcggt gggagatttt
     4561 ctgtacaaat tggttggaaa aatcgactat attggccctg ctcctatagt tttccaccag
     4621 ttcatataca gcggcaccct ttttattggc cagttcccat aggtattttg agctggagcc
     4681 acgaaattca aagatattct gatcgtcgtc ccctacagcg attacccgca tattatcgtt
     4741 gtattccatc aacgccttaa ttagactgaa ttcatcggca tccatatcct gggcctcgtc
     4801 gattaccaaa acagtttttg taatccttcc gggttcaaca tccccgtttc tgatcctgtc
     4861 tgttgcctcc tttattacct gtcccgacat ctccaggctt cctacccttc caagtagatc
     4921 aaagcaatag gaatggaagg tctttatctc cacaaaattg gccgcattgc cgatgagccc
     4981 tagaagcctt ttcttgaatt cagtagctgc tgcccttgag aaggtgacca taagtaattg
     5041 ctcatgctta acgtcctcca taagaaggag tgaggccagc ttatgaacca atattctggt
     5101 cttcccgctt ccgggtcccg ccgccacaac gatatatttt gattcactgt cattaataat
     5161 cttcaattgg gttggggaaa gatcaccgaa aagctgtcga aacttggcag gggtgatatt
     5221 ctttgtgatc tccttttggc ggtttccctt aaaatacttt ctcaaaaaag aactgtaatt
     5281 gagctggaaa taatcgtcta cgaattccag cgccgactta tagtcatgga tcatctttct
     5341 ggcatattcg cctacaatgt gtatctgctg caccttacta tcatagaatt gcttcaattt
     5401 ttcataatcc tggaccttat aacgctttct gttatccctt tcaatcctct ctatggtgag
     5461 ccccttatag atgaccataa aaccgccttc gattttcagg gcatctatct tagacagata
     5521 atacagggca tcttctatat ccttggatga aacctgggcc ttaataaggg tttgcctgtt
     5581 ttcgaaccct tccttaatgc ccagtaccga aaattctact aagacatcct cctggccatt
     5641 gggatctccc atgggctctg ttttgctttt ttcatatagg taccccagta taaagtccga
     5701 tagctcatag cgctttgcca gcctatcctc taaatcattc ctttcatgaa ggggcataat
     5761 cgctatatga ttcctggagt atccatgggt ctgctttttt atccaatttt taatggccca
     5821 gaagtttagt acagttttta tcttaccggg ggtcacgcct tcgcatccgc tctcctccgc
     5881 ccgctggtta aggtccttta tgtgaagggt ctgctcccca tcctctacgc aggacaacaa
     5941 aaatctttct atttgccaat aggatcttag gatttttctt gaatggtttt cgccctgacg
     6001 cccccttata aaggccgtaa gatccttggc atctgccaaa attccctctt ccctaagaag
     6061 gtttataatc tgtattactt cccccttggg gagccctaaa tggtccgata tatagtcaac
     6121 cctggattcg gcctcatcgc cttggtgttt actgctcttg ctggagaaaa gtcttctgat
     6181 tatcctaacc gccgatacct tttgatcctc catgaacctt tcagatgtct gaatcctttc
     6241 gatggcctca cgggcattct tcgttaggat gctattagca aatatcctgg gcgcattttg
     6301 cccccgtttt aggtagcccg catcctccaa ggccgcaata gccgccgtta ccctggtctc
     6361 aatatccacc acgctatcat cccagcctgc cttacgagca atctccagtg cagactgaga
     6421 taccttagat cgaaaccccg ttacatcctt gatagccttc catacctgct ggatctcttt
     6481 aatgctcagc ttcgtttgat ttaggaggat aaagtgtttg ctcagatcct catcattaaa
     6541 cagcacatag caatctgcag acatattttc gtccctgcct gccctacccg cttcttggat
     6601 atagctctct aaggaatctg aaatctcata gtggataacc atccccacat cctttttatc
     6661 cacgcccatt ccaaaagcgg aagttgccac catgatatca acctcacctt ttataaaggc
     6721 gtcctggttc tgggttttct cccccttgtc catctttccg tggtacggct tggcgggata
     6781 cccgtcttgg gatagtctct gggccagcat agacgccctt cttgtcctgg acacatatat
     6841 gatggtggga cactttttcc cttctatcag gtccctcacg gcgttgtatt tctcttcttc
     6901 cccttcttta ttaaagacca tatagcgaag attatccctg gatgtgcctg cagtgtataa
     6961 attaagctca agggacagct tatccttgaa gtatttttga atgtcgtcaa tcacattctg
     7021 cttggcagta gccgtaaagc aggatacggg gattccccca tcaatattct tcttttcctg
     7081 tatggacttt atgaagtcgc ctatatacat ataatcaact ctgaaatcct gaccccaggc
     7141 cgaaaagcaa tgagcctcat ctatgacgaa ccgcacaata tttctcccca ggagcaatcg
     7201 ctctatggtt ctggacctta aagattccgg tgagatgtat aggatggaag ccgatccctt
     7261 ctccaccctt tcaaaggatt ttgccctttc tatgggatct aataggccgt ttatggtaac
     7321 cgcctcggta atgcccacct cttctagatt atcgacctga tccttcatca aagattgcag
     7381 cggggagata accaccgtca gaccctttgt gctgtcccca cttatgaggg cgggaatctg
     7441 aaacgcaatg gactttcctc ccccagtagg aaatacagcc agtagggatt tattatctac
     7501 ggcggctttg acggccttct cctgcaaggg ttctcccccg tattccctat aagaatcata
     7561 cccaaaatag gttttaaggg cgttgtgtat atcgagggcc ttatggcagt acgtacagcc
     7621 ttcaaggcat ggcctgcccc gtagcatata cataacattt tctacccggg gaaagctttt
     7681 gagcacccat ggcggggtga tggaatatct attatttgca tttataaggg ccagacaata
     7741 ggccagttcg ataggatgtg cggatatcat gcccttaagg tcggcgtttt cacagatatg
     7801 ggggtgaaac ttttttttaa tcgccccttt tatgtctatt ttattcccag gattcgtctt
     7861 atccccgata tcatccctgt tttcaatgtc cgatccatta gccctgcact taataaaatg
     7921 gaagaatccc gaaaattctt tttcatcctt taagagatga tataggatgt ccctaaagcc
     7981 cttatctgta tggttataag ctgtgacctc gtcaaagaac aaatctctgg cctttatgga
     8041 atcgttgagt gggttgttta catcgtctga ttggagctta tcattcttta agagggcatg
     8101 gtatggcctg gcgggaaata agagggggga aagatacagg gtgtctatat atcttaaatc
     8161 atcgaattcc ttctgggtta tgacatcttc tatatacttt aaatcgtggt ttagaatgtt
     8221 atggccacag atatattgtg acccacacaa aaactttgca aattcgtcga cggatccaca
     8281 atgaaaagta tccccgttgg acttgatgct gcctatgtca agaattcttc cactatttat
     8341 gtcgacttca atgtctgcaa aagtgacaat cctcatattt ttcacctccc ggcgaatagc
     8401 gtagattacc tccaagaccc tagcgtttac gaccttatct taggttaaat tatagcattt
     8461 tgctttgttt tatgcaaata caagggcttt gatggggtgc ataatctaaa taaagtggca
     8521 gggcattcca tttgggcata acttgtgcta gaattatagt gagatttgca ttatcctggg
     8581 cctttgtgca ggttgaatgt atcagtaatg gctattgtac actgctaccg acgttctaat
     8641 atggctttac gggatgggcg tgaaaatatg gattttaagt ttttaataaa aaaggataaa
     8701 gcgtatatca catctctgtc aatatttttg gttttgatgt ggctgctttc aggatgttac
     8761 tctggtgaag gagccgatat attaaatcca gtggacgaat cgggaccccc ttactctggt
     8821 gccgtcataa atcctccaac ccatcactgc gaaagtgtcg atattgacat ggaaaaagag
     8881 ccggaggttg tgactgtgac aatcacggca accggggatt gtaccctggg catcaactca
     8941 aaaatggtct atccggagtc ctttgatgcc tattatgatg attatgggcc atcttatttc
     9001 tttcaaaatg ttagggacat cttgggggaa gacgatttta ccatagtaaa tttagaaggc
     9061 ccacttacca cctccaacga tgagcaaaca aagatacata accacaaagg gaaaccagaa
     9121 tatgttgaca tattaaggga aggctccgtg gaggcggtat ccctttcaaa caaccatacc
     9181 ttcgattatg gcatatcggg atatgaagat actgtaaatg ccctggatca ggcgggaatc
     9241 acttgggccg gggatgattc ttatggatta ttcgaggcca agggtgtaaa aatcgggttc
     9301 gtatccgtgg atgaacacta taacggcccc ttagttgagg tccgattaaa ggccggtgtc
     9361 gaggagcttc ggagacaagc cgccgatata gccattgcat gcatccattg ggggcgtgca
     9421 gaggacgaga acacctcccg tgtagacgat tatcaggtgg aactgggtaa aaagtgcatc
     9481 gactggggtt atgatcttat aatcggcaac catccccatg ccctacaggg tatagacagg
     9541 tacaggggaa aatatattac ctattgtctc gggaacttct gttacggtgg gaacaaaaat
     9601 ctaaaggacg gggacagtgg catattcagg caggtcttta cccttgtaga cggagaactg
     9661 caaacagatg ataatgtaca atttattccc tgcagttcat catctgtagc tgatgtaaac
     9721 gactaccgcc cgacaatagc ctccggtgcc gaagggcacc ggattatcac aaagatgaac
     9781 gcctattccg cacaatacgg tgtggtattt gatttgggcg gatgcataac aaccaagagc
     9841 ctgtccgacg gggacaggtc agacgcctat tattccatct ttcttgagtc catcagggag
     9901 gatgccttgg gcgatgatat agaatatgtt gccattgata tatcctcgtt ggatctatca
     9961 gactcctcct acctcatggg cttatttgaa atatgggcaa aggaatcgga tgttaaagtc
    10021 tttctaggca ctaaggagca gatagttgag gccggatacc acaactatgc gggagccggg
    10081 acaggcaggg tgtttatttt cagctcacca aaatggtcaa atctccaggt gacatgcagc
    10141 ctcagcaggc tggtgacaat gctcaactac atgagggacg attataccgc cgcctacgcc
    10201 aatgaggact gggtagcgca aaaggggggt tatatccaaa aataatttat tacagatcaa
    10261 cgatttcgac cataaaggtt acaggctgag caacaccaac gctaacaggc ctataatcaa
    10321 tccgattata gcttttttgc ccagcttttc tttgaagatt aggctggaag cgagggtgga
    10381 caaaaaaacc gttcccccat taatggaggg aaattgtact acgctgggga tcctggtaga
    10441 caggataagc gctaaccagt tgcctatggc cgtgctcacc ccggcaacga tgcttaccca
    10501 cgccaatgac caactcttaa aatgtgagac gtcctgtttg ttacgaaaga aattccacat
    10561 aaaaagaagc gaggagataa cagcggcggt accgaagcct atcactagaa attcgatggt
    10621 ttcctgccct tccatggtga cctgatgacc cttgctgata accataaggc ccccattccc
    10681 cacaaaggct ataagacaaa aaagaagcca ttttccaccc cattttttct ctccgtcttc
    10741 gtcagtggat tcgtttccta tataaaaggt tataaggagc aataaaagcc ccaacagttg
    10801 tagggggctg atgctctcgt tccaaaaaaa cagaccgtac aagatgggta ctaccagacc
    10861 gaaggcaaaa aatagcgcgg taaaggataa agggcccata cccatagcct tcatataaca
    10921 gaggatagtc ccgacgaaaa gggtaccaaa ggatatgccg agccataggg tatatggttt
    10981 taggccttta aagccgccca gtgccaaaag gatcaagacc accataccaa agtagataaa
    11041 gttgaataaa aaatagctgg cgatgttttt catatggctc ttgttaaatt ccttaaagct
    11101 gatggtctgg aacataaaga taaatataca agctataacc attacataat tcatcacaca
    11161 aatcccccca aaattaatat gtcaaaatgt ggccttgcca atccggcaaa aatatacata
    11221 gatacaatgc ataaaagcct tcattactgg ttcaactacg ccttctggtt ttgtgtaaaa
    11281 ataaatcaag caaatcagca taatggataa aaataagctt ttcgatatcg ggatactaat
    11341 gaagagtgtt taccgcacaa atataggtat accccgcgta catctaaaga attttttctc
    11401 cgtcaaaaat tatgatcccg aatccggccc cggtcttgtt accggcctta agccgatcaa
    11461 cataggccct ttgcagggat gtatcggtag gccatttatc gatatcctta tcgctgtctc
    11521 tgccaaaaac cctccatgcc tcggagaaat tgtcctcatc cactgaatat actatttcaa
    11581 aatcgtgcat aaaatcgatt atgtttgaac cctttggata aaacctttcg ttgtcgggat
    11641 agagaagcca tgaatggcaa accagcacaa tgggtcctcc attcagttca tccttaaaaa
    11701 aatcataggc ccttttgtaa gaatccattc gcttttcccg ggtcagaggt ccagccgagg
    11761 ggatatgcat gttatatacc ctgtcgccct tttctaccgc gtatccattt tttatgtatc
    11821 tatcacggga gaagctaatg ccctcgtatt gcatcctccc gaggccaaat ctttccatta
    11881 cgaaataatc cgaaagccaa ccggcaacaa aggtacccca aacaccatgg atatcacggc
    11941 attctctgag cttgtagccc agatccaaca tcgagttcca aaatatgtcc aaggaaaccc
    12001 ctgcggcctt atatttttct ttaagtattc ttgaacaata taataaaaac attaagtgca
    12061 ttgtgtatgg atttatatcc atcttctggc ccaagtgggt tagttcgttc aaaatttcgt
    12121 ctatttcttt gtgtccggca tcatagtagt acctgtgtat aatggaatcc atccctttgt
    12181 tatgggcttc atttgatgca aggctatcat gaacatccaa taaagcatca atggcctctt
    12241 ttgggaaatc aatcttttcc ataaaggacg ataaatactc tgttatctct ttcatatttt
    12301 catatccccc tttaactgtt tatttcaaga taaatctata ttgtgttctg gatattgtcg
    12361 gataattaaa tggaagatag catataaggc atataatgcg ggatccattt gtaatttata
    12421 cgatataacc ccacaaaatg agatgataac ccctttatta tgcaaatact tttactggcc
    12481 taatttgcta atgatactat acataattct tggatgtttt acaagtagta tatctggacg
    12541 aggggatatc ccccgtttgc atagggctct ttcaagcaaa taactgatac ggggttaacg
    12601 gaacattctt ctggaaattg taacggtaaa ataggggttt gacacctatt tatggttgct
    12661 ctataattaa atcagcccgt tgtattagct cattttagta tcggtgtttg tcaagaaagt
    12721 tgtcgtataa atttgatcta gatataagtt tccttttggc ataattccat tacggggttt
    12781 gctcgttaca gataaacaat aggtaaatta aatttatcaa tagatgaaaa gttttatagt
    12841 tggagttgtt gatctatcgt gatactatac ataatccaca tctcggtaat ggattgttaa
    12901 aagatgggtg actccattta atgaacaaaa taacgttggg ggtgtcggta tgggatctaa
    12961 gaaaaggata ttggacgcaa taaatcatag actaccagat agaattcccg tggattttgg
    13021 cggttctgct gtaacgggca tgcatgtcac ctgtgtggcc gccctgaggg atttctacgg
    13081 actggaaaaa agacccgtca agataaacga accctatcag atgttgggcc taatagagga
    13141 cgatctacag cagatcatcg gaattgatat ggtcccactg ggacaaaaga ataacatgtt
    13201 cggatttcct aacgataatt ggaaggaatg gcgcctccgt aacggacttg aggtattggt
    13261 gcccggggac tttaatataa ccgaagatga agccggcaat acctatattt accccgaagg
    13321 ggaccttacc gctccgccca gcggcaaaat gccaaaaggc ggctattatt tcgattctat
    13381 tattcgtcaa ccggagatta tagaagagga attagacccc aatgataacc tggaagagtt
    13441 taagccgtta aacgaagatg atatttcata ccttgtctcc gaggcaaaaa gaaccgccaa
    13501 aacaggtctg ggagttctgg ctaactttgg gggtactgcc cttggggata ttgctcttgt
    13561 accgggcccc tttatgaaac acccaaaggg cataagagac gttgaggaat ggtatgtatc
    13621 cacggtcatc cgaaaggact atatacacaa agtatttgat aagcaaatag atatagccct
    13681 tcaaagcttg aagcttatca atgaaagaat cggtgatttg gttgatatag cctatatctg
    13741 cggaacggac tttgggaccc agaccagcac cttctgctct gccgacacct acagggaact
    13801 ctacatgcca tactataaaa gggtaaatga ttggatacac gacaatacag attggaagac
    13861 ctttaaacac tgctgcgggg ctattgagcc cttcctaagt ctctttattg aatccggctt
    13921 tgacataatt aaccctgtcc aatgttcggc tgcaggcatg aaaccggaac aattaaagaa
    13981 gaaatacggt aaagacatag ttttctgggg cggcggcgta gatacccagt ggacgcttcc
    14041 cttcggtact cccgaggaag tccgaaaaca ggtcttaaat agatgcgaag tgttttccaa
    14101 agacggtgga tttgtgttcg gtgccataca caatgtccag gctaataccc cggtggaaaa
    14161 tatagctgct atgatagatg cagttcacga atttaattca atataatcgt ccgcttttca
    14221 acagttcatt acctttgtta ggcgacagta taacgagtcg gcaagatgtc ttccgcttct
    14281 ataagcggcg agttcccatt agtaaaatag gcggtgggta taaatcaccc gcctcgttga
    14341 aacctgtgtg ttgcaattgc ttctgatgtt ttaacgcatt agcatattat aaggcttatt
    14401 caatttgaaa agcctatggt ttccaatccg gcctcctgtc ggcggaatcg gcttaaggcg
    14461 tcttgtttgt tgagggacaa ggcatgacaa acagaacccc actaaaaaag gaacttctga
    14521 taggcgaggc tcccctgctt aggattatcg aaatatttga tataaagaaa atagttgcgg
    14581 tgggcaataa ggcggacgaa agccttacga ggctcggaat cgaccatgag aaggtgagac
    14641 atccggccca gggggggaag aatgaatttg tcaggggaat aagagccatc aaaaaagggt
    14701 tgatgtccga taatcccatg tcccgtcata cacgctttgt caccacctga atataagtgc
    14761 aaaaagtatc gttggcaaca tattcgccac ccttattttt ttgtcccaaa ccagatttac
    14821 gccaacacaa aaaatcagca tcgacgctgt cagggaaaga ttggacatag ccctcactgt
    14881 cattagaggc tcaataaacc ctgccaaaat cgttatggta ccctgcaata gcgcaaccgg
    14941 gatggccgca aagatacacc ccttgcctat ggaagctgcc atgatcatga ctattataaa
    15001 atccagaact gattttgcca taagcagtga ataatcgccc gaaagtccat cctgtatagc
    15061 gcctacgacc gccattgctc caacacagac tatgagggat gtggatacaa aaccgtccat
    15121 aaactttgta tccttttcgt ttctcgtctt cgctctcagc caggcaccga attgttctat
    15181 tctcctctcc aaattcactc cctcacccag aagggagccc agaacgaagc tgaatataat
    15241 catcatgctt ccgccgctta ccagttttcc gccctcgtag gatagcatct cttccagtgc
    15301 tccacctatc cctatgaata acacacatat tccgatggct tttataaggg tgttttggat
    15361 tctttcggag aaggcattct taagcgtgat ccccaagagc ccgcctagga taatggcccc
    15421 ggtattgact aatgttccta ttcccatatt ctaatgtatt cctttccgtg gatgtattga
    15481 tgtgtataat gcattagaac aattttatga tactttcggg cagtttgcaa ttgtatattt
    15541 tcttttcaga aagaataagc cccagttttt caaaaacaat atcagaaata tagaagtttc
    15601 aaataataca ttattaataa cctctgttat tgtagtatta tttttttttg aaacaccaac
    15661 tgcccagtta ctatcgcgag gtaactaggc gttcaatagc cattgccgga atgcttaac
//
LOCUS       DASRNX010000089         3959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_69065,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000089 DASRNX010000000
VERSION     DASRNX010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69065"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFD89_03505"
     CDS             <1..209
                     /locus_tag="VFD89_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57289.1"
                     /translation="HKFKTVRRNKIIVEKILFPGYNTPGGYSIAEGICFINYGENYIL
                     MRPSLPRYRCLEEGLYNGSPPTFV"
     gene            254..2728
                     /locus_tag="VFD89_03510"
     CDS             254..2728
                     /locus_tag="VFD89_03510"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZJ57290.1"
                     /translation="MANKKEVLKIDGMTCTACANAVERRVGRLEGVDTANVNFATEAL
                     TVVHGDGADLGAIKKAVEEAGYSVREEQNNREVVIPIEGMTCASCSRAVERALDKLEG
                     VKTGDVNLATNRAKVVYNPEVVRLSQIREAIVKAGYKPMEIQTKTQGDTDKIRREKEI
                     SVMWTKFIVSLIFTTPLFYIAMGHMIGFPVPGFMNPDTNPLNFALVQLFLTIPTVIAG
                     YRFYTVGFRTIFKGSPNMDSLVAMGTSAAFIYGLYAIWQITQGNTDYAMHLYFETAGV
                     IITLILLGKTLETVAKGRTSDAIKKLMDLTPKMATVIHDGGEITIPVEEVEVGDIIVV
                     KPGQSIPVDGQVTDGLTSVDESMLTGESIPVEKKAGDNVVGGSLNKTGSFRFKATKIG
                     SDTALAQIIKLVEEAQGSKAPIAQMADIISGYFVPIVFGIAVVAGLGWWLSGESAVFS
                     LTIFISVLVIACPCALGLATPTAIMVGTGKGAENGVLIKSGGALETAHKVDTVVLDKT
                     GTITLGQPKVTDILLYGEVEEDQLLRFAASAEKGSEHPLGESIVERARELDMDLYTPD
                     SFNAIPGHGIEAGISGKDLLLGNKRLMDDRQIELGEAISDSERLAKEGKTPMYVSIDG
                     RLAGIIAVADVLKESSKDAIRALHKLGLQVVMVTGDNRRTAEAIAEQVDIDMILAEVL
                     PQDKALEIKKLQDSGKKVAMVGDGINDAPALAQADIGIAIGSGTDVAMESADIVLMKS
                     DLMDVSTAIELSKMTITNIKQNLFWAFGYNTAGIPIAAGLLHLFGGPLLNPMIAAAAM
                     AFSSVSVVSNALRLKRFRPGSTAETA"
     gene            2777..2995
                     /locus_tag="VFD89_03515"
     CDS             2777..2995
                     /locus_tag="VFD89_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HZJ57291.1"
                     /translation="MIKKKLSIKGMTCQHCVMHVANALKELDGVEGVDVNLKGQYSVV
                     ELSKEIDEAAFTEAIEDAGYDLVGVETV"
     gene            3069..3332
                     /locus_tag="VFD89_03520"
     CDS             3069..3332
                     /locus_tag="VFD89_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HZJ57292.1"
                     /translation="MHREKNDPVFRNLKIARGQVDGIIKMLEDDRYCIDVSRQIMATI
                     SILRKVNAEILHDHMKGCVRRAILDDGGDDSLEEVFEIIRKYF"
     gene            complement(3329..3598)
                     /locus_tag="VFD89_03525"
     CDS             complement(3329..3598)
                     /locus_tag="VFD89_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57293.1"
                     /translation="MPNIKEAMDRIRILQCPTGQLEERVANILEDHNISPKSEILISR
                     EKRLDKDGAEAYTVTISDRLDPAITLLAKSGIDDYVVQIKDIYFN"
BASE COUNT         1135 a          814 c         1062 g          948 t
ORIGIN      
        1 ggcataagtt taaaactgta aggcggaata aaataatagt tgaaaaaata ttattcccgg
       61 gatataatac ccccgggggg tatagtatag ctgagggaat ttgtttcatc aattatgggg
      121 aaaactacat tttaatgaga ccgagtctac ccaggtatag gtgtttagaa gaaggtctat
      181 acaatggaag tccccccaca tttgtataaa tacattcgag cagcggccaa tatattttgt
      241 aggagttgat tatatggcta ataaaaagga agtactaaag atagacggaa tgacctgtac
      301 cgcctgtgcc aacgccgttg aacgcagggt aggcagactt gagggggtag atacggccaa
      361 tgttaatttt gccaccgagg ccctaaccgt ggtacacggg gacggggcgg accttggtgc
      421 cattaaaaag gctgtggagg aggcaggcta cagcgtaagg gaagagcaga ataaccggga
      481 ggtcgtaatc cccatcgagg ggatgacctg cgcatcttgc tctagggctg tagaaagagc
      541 attggataag ttggagggag tcaagacggg agacgtaaat ttagctacga acagggcaaa
      601 ggttgtatac aacccggagg tagtcaggct gtctcagata cgagaggcaa tcgttaaggc
      661 cgggtataag cccatggaaa tccagacaaa aacccagggg gatactgata aaattcgaag
      721 ggagaaggag atctccgtca tgtggaccaa atttatagtg tccctgatat ttacgacccc
      781 gctattttat atagccatgg gccatatgat aggatttccg gtacccgggt ttatgaaccc
      841 cgacaccaat cccttaaact tcgcccttgt tcaattgttt ctcacgattc ccacggtaat
      901 tgcggggtat agattctata cagtcggctt cagaactatt tttaagggca gcccaaatat
      961 ggactccctt gtggctatgg gtacatccgc tgcatttata tacgggctct atgcgatatg
     1021 gcagataacc caaggcaaca cggattatgc catgcatctg tactttgaaa ccgcgggggt
     1081 cataatcacc ctgatactct tgggtaagac tttggaaacc gtggccaagg ggcgtacatc
     1141 cgatgccatt aaaaagctta tggaccttac cccgaagatg gcgacggtaa tccatgacgg
     1201 aggggagatc accatacccg tagaggaagt agaggtcggg gatatcatag tggtaaagcc
     1261 cggacagagc attcccgtag acgggcaggt gacggatggg ctgacttccg tggacgaatc
     1321 catgctgacg ggggagagca tacccgttga aaagaaggca ggggacaatg ttgtgggcgg
     1381 cagcctcaat aagacgggct ccttccgctt caaagccact aagataggca gcgacacggc
     1441 gcttgcccag atcataaagc tggtggaaga ggcccagggc agcaaggccc ccatcgctca
     1501 gatggcggat attatatccg gatactttgt gcccatcgtg tttgggatag cggtggtagc
     1561 ggggcttggt tggtggctca gcggcgaatc ggcagtattc tcccttacca tctttatatc
     1621 cgtcctggtt attgcctgcc catgtgcatt agggctggca acccctacgg ccatcatggt
     1681 aggaacgggg aaaggggcag agaacggcgt tcttataaag agcggaggag ccttggaaac
     1741 ggcgcataag gttgacaccg tagtccttga taagactggg acaattaccc tggggcagcc
     1801 caaggtaacc gatatactct tatacggcga ggtagaagaa gaccaactct taaggtttgc
     1861 ggcatctgcg gaaaaaggtt ccgaacaccc cttgggagaa tccatcgttg aaagggcaag
     1921 ggagctggat atggatcttt atacgcccga cagctttaac gccatacccg gacacggtat
     1981 cgaagccggg atctcgggaa aggatcttct ccttggcaat aagcggctga tggacgacag
     2041 gcaaattgaa ctgggcgagg caatatccga ttccgaaaga cttgctaaag aaggcaaaac
     2101 tcctatgtat gtaagcatag acgggagact tgcaggcata atcgccgttg ccgatgtgct
     2161 caaggagagc agcaaagatg ccataagagc ccttcataag ctgggccttc aggtggtaat
     2221 ggttaccggg gataacaggc gtactgcaga agcgattgca gagcaggtgg atatagatat
     2281 gatccttgca gaggtattgc cccaggataa ggctctggag ataaaaaagc tccaggattc
     2341 cgggaaaaag gttgccatgg taggcgacgg aatcaacgat gcgccagcct tggcccaggc
     2401 ggatataggg attgccatag gctcgggcac cgatgtggct atggaatctg ccgatatcgt
     2461 actcatgaaa agcgatttga tggacgtatc caccgccata gagctgagca agatgaccat
     2521 aaccaatata aaacaaaatc tattttgggc ttttggatat aatacagccg gaatacccat
     2581 tgccgcggga cttttgcacc tgttcggcgg accccttctc aatcccatga tcgcagcggc
     2641 agcgatggcc ttcagctcgg tatccgtagt cagcaatgcg ctgaggctta agaggttcag
     2701 gcccggatca acagccgaaa cggcttaaag ggtgtacaaa ataaggattt ttccaaaaca
     2761 tgaatggagg acaataatga taaagaaaaa actatctatt aaaggtatga cctgccagca
     2821 ttgcgtaatg catgtggcaa acgccttgaa ggaactggac ggggtggaag gcgtggatgt
     2881 caacctaaag ggacaatata gcgtggtgga actttcaaag gaaatcgatg aggcggcctt
     2941 cactgaggcg attgaagatg caggatatga tcttgtaggt gtggaaacag tatgaaaagt
     3001 ttagagggat gcccaaaatg cggcatccct ctaaaaacaa attctcttta gggttggggg
     3061 gggaaaacat gcatagagaa aaaaacgatc ccgtattcag aaacctaaaa atcgcaaggg
     3121 gtcaggtaga cggtataatc aagatgctgg aagacgacag atactgtata gacgtatcaa
     3181 ggcagatcat ggctacaata tctatactca ggaaagtcaa tgcggaaatc cttcatgatc
     3241 acatgaaagg ttgtgtcaga agggccattc tcgatgacgg cggcgatgac agcctggagg
     3301 aagtatttga gataataaga aaatacttct agttaaagta tatatccttt atctggacca
     3361 cgtagtcgtc tattccggat ttggccagaa gggtgatggc gggatccaac ctatcggaga
     3421 ttgtgacggt atacgcctcg gcaccatcct tatccaacct cttttctctg cttattagga
     3481 tttcactttt aggggaaatg ttatgatcct ccagaatatt cgctacccgc tcttccagtt
     3541 gaccggtggg gcattgcagt attcttattc tgtccatggc ctcttttatg tttggcatac
     3601 agattcctcc ctattattat ttttgatttc aatcaaccaa gtgcaaaagg gatactgatg
     3661 tatcagcgct tggttgattt tatgaaaagt tttatggttt tattccggcc agtagtcata
     3721 catttattaa tgcctatttc tgatatcgct tattcattcg ttcataattt cggtgcggat
     3781 acccgtgact ttagtcatgg gctattgaca ctttgatgag cgggatataa aaaacatcgt
     3841 ctaatggtgg attaaaacgc tgataattct tgccgtattc aaaaatgcgg tggagcataa
     3901 cggtaattat ataaaaaaag gtgattgaat aattataagg gtgggtatat aataatgaa
//
LOCUS       DASRNX010000090         9898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_388547,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000090 DASRNX010000000
VERSION     DASRNX010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9898
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388547"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..931)
                     /locus_tag="VFD89_03530"
     CDS             complement(11..931)
                     /locus_tag="VFD89_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZJ57294.1"
                     /translation="MKSKRRTLGRITTEDVIFDAVNYIILIFLCVATLYPFLNTLAIS
                     LNDATDAIRGDIYLWPREFSWRNYEMILTENTNILRALFISGARAVVGAGTSVFACLM
                     IAYTLSRKDFVLRRFFIKFLVLTMYFSGGMIPVYLLMRSLGLINNFLVYILPGMVGAY
                     NVMVMRSYIEGIPESLVEAAKIDGASEYRILFTIIMPISMPVIATIILFVAVGQWNSW
                     IDTMLYTSSRQELSTLQFELQKLLSSTRTMTSATADSAEALGLLEKGIVTPNSLRAAM
                     TIIATVPILFSYPFLQKYFVQGLTLGGVKG"
     gene            complement(954..1889)
                     /locus_tag="VFD89_03535"
     CDS             complement(954..1889)
                     /locus_tag="VFD89_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZJ57295.1"
                     /translation="MSQVARANKKSFWKQLVQQRQLLVLSVPFIILVFIFNYVPIWGW
                     ILAFKNYNPGLGFLKSPWVGLDNFREVFADSNFYRALKNTLAISSLKLVFNFTSAIVL
                     AVLLNEVKNILFKRTVQTISYLPYFVSWVVAANIVFMSLSPEDGIVNVLLMSMGILKD
                     PIPFMGMKEYFYWVVALSSVWKTVGYSAIIYLAAMTGIDPELYESAEIDGAGRLRKIF
                     AITIPSIMPTIKILLVLNIGWIMNAGFEQVYLLGNASVQEVSNTLEVYVYNYALKYFR
                     YSFGTAIGIFNSIAAFVLITVSNFVSKKVDGEGVL"
     gene            complement(2088..3836)
                     /locus_tag="VFD89_03540"
     CDS             complement(2088..3836)
                     /locus_tag="VFD89_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZJ57296.1"
                     /translation="MNSKRFIAMLVAFLLVVVVVAGCGQTKEQPTKTDPDKSGEVKEE
                     KPQEEKPQEEKPQEEKPKEVTTYTAFFNYALTSMIDPWETPIGKKITELTGVKLKTEY
                     LVGTDARQKAGVMIASGDYPDIIIPGESTGDYVAAGAFVPLDDYLEEHGENIKQIYRP
                     SELQLLKMQHGTTYYISGARAGEDTLYPAAGFYLVADAVEKAGWPVIKTLDEYAEILK
                     DYVQENPEYNGKSTIAFTLPGEGWRMSGLQYGAARFLDGYPNDGPACVDQETLEAKAI
                     VTQDFNLTFLKFVNELWNMGIADKEMFMQTNDQYLAKISSGRVAGVYDQRSMFIDGLN
                     ALEKQELYDRMLIAFPVVNEGVEREYYRGPRAFSTQSGIAISTKAKDPEAIFQFLDRM
                     GAEDINKLNKWGIEGEDYTMEDGKPVKTQEQWDKYNDAEYQRKQGINQFGDYPRREDV
                     DSDYGRFSDGNLVSPTLFDEFFEVRYKDYEKEILKNFGIKTFNDFYAPSYPATYEPGW
                     AIRTKMPADFSGKIAVEQGLELATEYLPKVIMAKTEDFDKVWNEYQDKLSKLDLKSFE
                     EEMTKELKAGAQYYQD"
     gene            complement(4781..5701)
                     /locus_tag="VFD89_03545"
     CDS             complement(4781..5701)
                     /locus_tag="VFD89_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZJ57297.1"
                     /translation="MRVKTKGLSRITAEDVIFDTVNYIILIFLCVATLYPFLNTLAIS
                     LNDATDAIRGNIYLWPREFSWRNYEMILTENTNIGNALFISAARAVAGAGTSVLACLM
                     IAYTLSRKNFVLRKFFIKFLVLTMYFSGGMIPVYLLMRSLGLINNFLVYILPGMVGAY
                     NVMIMRSYIEGIPESLVEAAKIDGASEYRILFTIIMPISMPVIATIILFVAVGQWNSW
                     FDTMLYTSSRQELSTLQFELQKLLSSTQTMTSSSADAADAMGLLDKGIVTPNSLRAAM
                     TIIATVPILFSYPFLQKYFVQGLTLGGVKG"
     gene            complement(5725..6660)
                     /locus_tag="VFD89_03550"
     CDS             complement(5725..6660)
                     /locus_tag="VFD89_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003323031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZJ57298.1"
                     /translation="MNRVARSKKSRFWEQLVQQRQLLVLSVPFIILVFIFNYVPIWGW
                     ILAFKNYNPGLGFFKSPWVGLDNFKEVFTDSNFYRALKNTLAISSIKLVFNFTSAIVL
                     AVLLNEVKNIIFKRTVQTISYLPYFVSWVVAANIVFMSLSPEDGIVNHLLVFLGILKE
                     PIPFMGMKEYFYWVVALSSVWKTVGYSAIIYLAAMTGIDPEIYESADIDGAGRLRKIF
                     AITIPSIMPTIKILLVLNIGWIMNAGFEQVYLLGNASVQEVSNTLEVYVYNYALKYFR
                     YSFGTAIGIFNSIVAFVLITVSNFVSKKVDGEGVL"
     gene            complement(6860..8578)
                     /locus_tag="VFD89_03555"
     CDS             complement(6860..8578)
                     /locus_tag="VFD89_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZJ57299.1"
                     /translation="MKCKRFITMLIALILVIAVLAGCGQTKEQSGKADPPQSGNGKEE
                     TPQDEKPGEVTTYTAFINYALTSMTDPWETPVGKKITELTGVKLKTEYLVGTDARQKA
                     GVMIASGDYPDIIVPGESTGDYVAAGAFVPLDDYLEEYGENIKQIYRPSELELLKMQY
                     GTTYHISGVRAGEDTLYPTAGFYLVADAVEKAGWPVIKTLDEYADLLKDYVQKNPEYN
                     GKPTIAFTLPGEGWRMSGLQYGAARFLDGYPNDGPACVDQETLEAKVIMTQDFNLTFL
                     KFVNELWNMGIADKEMFMQTNDQYLAKISSGRVAGVYDQRGMFIDGLNALEKQELYDR
                     MLIAFPVVNEGVEREYYRGPRAFATQSGIAISTKAKDPEGIFKFFDRMAAEDVNRLNK
                     WGIEGEDYTMEDGKPVKTQEQWDKYIDADYQKKQGINQFDTFPRREDVDSDYGRFSDG
                     NLVSPTLFDDFFEIRYKDYEKDILKNFGIKTFNDFYAPSYPATYEPGWAIRTKMPADF
                     SGKIAVEQALELATEYLPKVIMAKTEDFDKVWSEYQDKLSKLDLKSFEDEMTKELKAG
                     AQYYQD"
     gene            complement(8847..>9898)
                     /locus_tag="VFD89_03560"
     CDS             complement(8847..>9898)
                     /locus_tag="VFD89_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZJ57300.1"
                     /translation="QDHYKNRLSGLDPVELENRGFHVRQLLVDIIGEENARNLFEEGD
                     FRYGIITGRKILDQYHGNMEELAQRIREKINLEAQIDASVFVGEQVKGLKALGRSHKS
                     SLTAMHHRFFRGGNCIIFYDEIKGITFNYGFKEVLSFPALVEAIENDKADEVYSQVDA
                     LFIKLIKLFLAPEIIQAYLNNFALEIAKIITQMDGNMDELVEEFTVLNMDIDKITIDV
                     LKEVLTRFCIKGAAYIKTLRERQSMGPIAKVEDYIRQNFREDITLKGIAEEFFMNPVY
                     LGQLFKKTFGIYFSDYLHNLRVEEAKKLLRRTDMKVYEIAADVGYRDTDYFVSRFAKE
                     VNMTPSQYKKSLIGD"
BASE COUNT         2721 a         2297 c         1930 g         2950 t
ORIGIN      
        1 gtgacatcat ttatcccttg accccaccta aggtcagacc ctgaacaaag tatttttgca
       61 ggaagggata tgaaaacaga ataggcaccg ttgctataat ggtcatagcc gctcgcaggg
      121 aattaggcgt cactatcccc ttttccaaca gacccagggc ctctgccgaa tccgccgttg
      181 cagatgtcat agtccgggta gaggataaga gcttttgcag ttcaaattgc agtgtgctca
      241 gctcctgcct gctggaggtg tataacatgg tatctatcca tgaattccat tgcccaacag
      301 ccacaaaaag gatgatggtg gcaatgaccg gcatgctaat gggcattata atggtaaaca
      361 atatcctata ttcgctggcc ccgtctattt tggccgcttc taccaggctc tcaggaatac
      421 cttctatata gcttctcatg accatgacgt tataggctcc taccatcccc ggcagaatat
      481 atacgaggaa gttgttgata agccccagag atctcataag gagatagacc gggatcatac
      541 caccgctaaa gtacatggtg agaaccaaga atttgataaa aaatcttctg agtacgaagt
      601 ccttgcggct tagggtatag gctatcataa gacatgcaaa cacactggta ccagccccta
      661 caaccgccct ggccccggat ataaataggg ctcttaagat attggtgttc tccgttaaaa
      721 tcatctcgta gttgcgccag gaaaattctc tgggccaaag atatatatcc cccctgattg
      781 catcggttgc atcgtttaag gatatggcca aagtgttcaa aaatggatat agggttgcta
      841 cgcacaggaa aatcaggata atatagttca ctgcatcaaa tattacatcc tccgtcgtta
      901 ttctgccaag agttctcctc ttagatttca tactaccgcc cccttaactt taatcataat
      961 acaccttccc catctacctt tttagacaca aagttggata cggttataag cacaaatgct
     1021 gcgatggaat tgaagatacc gattgctgta ccaaaggaat agcgaaagta ttttagtgca
     1081 tagttataca catagacttc taaagtattg gacacttcct ggaccgatgc attccccagc
     1141 agatagacct gttcaaaacc agcgttcata atccagccta tgttgagaac caataatatt
     1201 ttgatagtgg gcattataga tggaatggtg atggcaaata tctttctgag ccttcctgcg
     1261 ccatctatct cggcagactc gtagagttcg gggtctatcc cagtcatagc ggccagatag
     1321 atgattgcac tataccccac tgtcttccat acagaggaca gggccactac ccaatagaag
     1381 tactccttca ttcccataaa gggtatgggg tctttgagga ttcccatgct cattaggagc
     1441 acattgacaa taccgtcttc cggggacagg gacataaata cgatattggc cgctaccacc
     1501 caggatacga aatagggcag gtaggagatg gtctgcacgg ttcttttaaa taggatgttc
     1561 ttgacttcat ttaaaagcac ggccaagaca atagccgatg taaaattgaa taccagctta
     1621 aggctgctta tggctaacgt gttcttgagt gccctataaa aattactgtc agcgaatact
     1681 tctctaaaat tatccaggcc tacccaaggg gattttaaaa atcccaggcc cgggttatag
     1741 tttttaaaag ctaaaatcca cccccaaatg ggtacatagt tgaatatgaa tactaaaata
     1801 ataaaaggta cagataatac aagaagttgc cgctgctgaa ctaattgttt ccagaagctc
     1861 tttttatttg ctcttgctac ctggctcata caatacctcc gtattatttg taggaaagta
     1921 caccattttt gatgtacttt cctacaaagt cttatttagt ctgcccatat gccccgcacc
     1981 catagctccc gcggagatct atgggataca gaacacttgc tgtaacttac atgatacgaa
     2041 aacgctccaa gttatagtat caagcccctt cactacatgc gtagctacta gtcctgataa
     2101 tactgggcac cggccttaag ttccttggtc atctcctctt caaaggactt aagatccaat
     2161 ttggacagct tatcctggta ttcgttccat accttatcaa agtcttccgt cttggccata
     2221 atgaccttag gcagatattc ggtagccaat tccagacctt gttccactgc aatttttccg
     2281 gagaagtcag caggcatctt ggttctgata gcccagccgg gctcgtaggt tgccgggtag
     2341 gaaggcgcat agaagtcgtt aaaggtcttg atgccaaagt ttttaaggat ttctttttcg
     2401 taatccttat atcttacttc aaagaattca tcaaataggg tgggagatac caggtttccg
     2461 tcggagaatc tgccgtaatc ggaatcgacg tcctctcttc ttggatagtc accaaactga
     2521 tttatcccct gttttctttg atattcggca tcgttatact tatcccattg ttcctgggtc
     2581 ttaacgggct tgccatcctc catggtatag tcttcgcctt caattcccca tttgttcaac
     2641 ttattaatat cttctgctcc catacggtcg aggaactgaa agatggcttc aggatccttg
     2701 gccttggtgc tgatagcaat accggactgg gttgagaagg cccttggtcc tctgtaatat
     2761 tctctctcta ccccctcgtt tacaacgggg aaggcgatga gcattctgtc atagagctcc
     2821 tgtttttcca gagcattgag accgtcaatg aacatactcc tctgatcata tacgccggct
     2881 acacgaccgg agctgatctt ggccaggtat tgatcattgg tctgcatgaa catctccttg
     2941 tctgcgatac ccatattcca tagttcgtta acgaatttca agaatgttag gttgaaatcc
     3001 tgggtcacaa tggccttggc ttccaaggtc tcctggtcta cacatgcggg accgtcattg
     3061 gggtatccgt ccaagaatct agcggcacca tattgcagtc cggacattct ccagccttcg
     3121 ccgggaaggg taaatgcgat ggtggacttg ccgttatatt cgggattttc ttgaacgtag
     3181 tcctttagaa tttctgcata ttcgtccaat gtcttgatta ccggccatcc cgccttttct
     3241 acagcgtcgg ctaccaggta aaaacctgct gcaggataca gggtatcctc acctgcccta
     3301 gctccgctaa tgtagtaggt ggttccgtgt tgcatcttta acagctgcag ttccgagggg
     3361 cgataaattt gcttgatatt ctccccatgc tcctccagat aatcgtccaa tggtacaaaa
     3421 gcgcctgccg ccacatagtc acctgtggat tcgccaggga tgatgatatc gggataatcc
     3481 ccggaggcaa tcataacgcc ggctttttgt ctggcgtcag ttccgaccaa gtattccgtc
     3541 ttcagcttta caccggttag ctcggtaatc tttttaccga tgggtgtctc ccaaggatcg
     3601 atcattgagg tcagagcata gttgaaaaac gctgtgtaag tggtgacttc cttgggcttc
     3661 tcctcttgcg gcttctcctc ttgcggcttc tcctcttgcg gcttttcttc tttcacttcc
     3721 ccggatttat ccggatcagt ctttgtaggt tgttccttcg tctgaccgca tccagcaact
     3781 acgacaacaa ccagaagaaa ggcaacaagc atcgcaataa accgtttgga attcattaaa
     3841 tctcctcctt ttatttatct atgatatttg tacttttaag aggtccctaa atgtacaaat
     3901 cccatacttt tattcaacag ttgattttac ttgggaagtc ttaaagaata tacaattcaa
     3961 tattttggaa tatagcgtta ataattcaag cctttttgaa cctcaactta cgaattgttt
     4021 atttatgatg cactgagcgg cgatggaccc atttatccag gataaactcg gttaatacag
     4081 tgaaaccaat agccccatat aaagaatata ttaagggtag atatataatg tatactcata
     4141 ctatgttcgg gatattattg aaagacttat tttaaagtcc cgaaaaaatc aaatagtctg
     4201 cactgcaacc taacgcgcct attaatgtat cttttgaaac cttataattg cctgttgata
     4261 tacaaaggag taaaaatatt cagtcagtag aagaatttaa aagatatgtt ctgtaccata
     4321 aaaacaaaga tgttattagc gccaatgtct tgttataaaa cagattgttt tcgcctaatt
     4381 gtagtatttc agttcgaatg atgttttata aaattactta attaattata atagctatcc
     4441 tatatgtcta ggataggtcg taattgttgt catttattct atttaattat ataattgcac
     4501 cttttactaa acaacgggaa aaactatagt tctttccgta aatatcttag gtttttccta
     4561 tttgtcctca cagtatgacc tgggggtagg ttcataaaaa cagcaagcct cactaccgat
     4621 gaagagattg cataaaaata ttataacgat ttataaaaca tgccgccaaa aatagaaatt
     4681 cccgaactct gtccgtggca gacccattat ctccttggat aacggcttac cggccaggtt
     4741 cgggattcga agttaattcc cctatataag gtgacaccat ttatcccttg accccaccta
     4801 aggtcagacc ctgaacaaag tatttttgca ggaagggata tgaaaacaga ataggcaccg
     4861 ttgcaattat agtcatagcc gcccgaaggg aattaggcgt aactattcct ttatccaaca
     4921 ggcccatagc atcggctgca tcggctgagg atgatgtcat agtctgagta gaggataaga
     4981 gcttttgaag ttcaaattgc agggtactga gttcttgtct gctggaagtg tataacatgg
     5041 tgtcaaacca tgaattccac tgaccaacgg ctacaaatag aatgatggtg gcaataaccg
     5101 gcatgcttat aggcatgata atagtaaaca atatcctata ttcactggcc ccgtctattt
     5161 tagccgcttc taccagactt tctgggatac cttctatata actcctcatg atcatgacgt
     5221 tgtaggcgcc caccattccc ggcaaaatat acacgaggaa gttgttgata agacccaaag
     5281 acctcattaa tagatagacc ggaatcatgc cgccgctaaa gtacatggtg agaaccaaga
     5341 atttaataaa aaactttctg agtacgaaat tttttctgct tagagtatag gctatcataa
     5401 ggcaagccag cacactggta ccggctccgg caactgccct agccgctgat atgaataaag
     5461 catttccgat atttgtattt tcagtcagaa tcatttcata attgcgccaa gaaaattctc
     5521 tgggccacag ataaatattt ccccggattg cgtctgttgc atcgtttaag gatatggcta
     5581 atgtatttaa aaaaggatac agggttgcca cacataagaa aatcaggata atatagttta
     5641 ctgtatcaaa tataacatct tcagctgtta ttctgcttag acctttcgtt ttaaccctca
     5701 taccaccgcc cccttctgct tttatcatag tacaccttcc ccatccactt tcttggacac
     5761 aaaattggat acagttataa gtacgaatgc tacgatagaa ttaaaaattc caattgctgt
     5821 accaaaggag tagcggaagt attttagtgc atagttatat acatatacct ccagtgtatt
     5881 tgacacttcc tggaccgatg cattacccaa tagatagacc tgctcaaatc cagcgttcat
     5941 aatccaaccg atatttagaa ccaataatat cttgatggtg ggcattatgg agggaatggt
     6001 gatggcaaat atcttcctaa gtctcccggc cccgtcgata tcagcggatt cataaatttc
     6061 ggggtctata cccgtcatag cagcaagata aataattgcg ctatatccca ctgttttcca
     6121 tacagaggac aaggctacga cccaataaaa atactccttc attcccataa aagggatggg
     6181 ctcttttaag attcccagga aaactaaaag atgattgacg ataccatcct ccggggacag
     6241 ggacataaat actatattag cagccaccac ccaggataca aaatagggta ggtaggaaat
     6301 agtttgcaca gttcttttaa atataatatt cttgacctcg tttaaaagca cggctaagac
     6361 aatagccgat gtaaaattga ataccagctt aatgctgctg atagccagtg tatttttgag
     6421 cgccctataa aaattactgt cagtaaatac ttctttaaaa ttatccaggc ctacccaggg
     6481 agatttaaaa aatcctagtc cagggttgta gtttttaaaa gctaaaatcc atccccaaat
     6541 cggtacataa ttgaatatga atacaaggat gataaaaggt acggataata caagaagttg
     6601 ccgctgctga actaattgtt cccaaaatct actttttttt gatcttgcta cccgattcat
     6661 aggatacctc cgtattattg ataggaaagt acatcatttt ttgatgtact ttcctacaaa
     6721 gtcttattta gtctgcccgt ttgccctgcg cccagagcct ccattggagt ctataggatg
     6781 cgaaatattt gctgtaacct gcatgttacc aaaacgcttt taattacagt gttaagtgct
     6841 ttcgctacat gcatggctgc tagtcctgat aatactgggc accagcctta agttccttag
     6901 tcatttcatc ttcaaaggac ttaaggtcca atttggacag cttatcctgg tattcgctcc
     6961 ataccttatc aaagtcttcc gtcttcgcca tgatgacctt aggcagatat tcggtagcca
     7021 gctctaaagc ttgctcaact gcgatctttc cggagaaatc ggcaggcatc ttggttctga
     7081 tagcccagcc aggttcgtag gttgccggat aggatggtgc atagaagtcg ttaaaggtct
     7141 taataccaaa gtttttaagg atgtcttttt cataatcctt gtatctgatc tcaaagaaat
     7201 catcaaacag agtaggggat accaggtttc cgtcggagaa tctgccgtag tcggaatcaa
     7261 catcctctct ccttgggaaa gtgtcaaact gattaattcc ctgtttcttt tgatagtcag
     7321 catcaatata cttgtcccat tgttcctgag tcttgacagg ctttccatcc tccatggtat
     7381 agtcctcgcc ctcaataccc catttgttca gcctatttac atcctctgct gccatccgat
     7441 caaagaattt aaagattcct tcgggatcct ttgctttagt acttattgca ataccggact
     7501 gggttgcgaa ggcacggggt ccgcgatagt attctctttc cactccctca tttaccacag
     7561 ggaaggcaat gagcattcta tcatagagtt cctgcttttc taaggcgttt aaaccgtcga
     7621 taaacatacc cctttgatca tatacaccag ctacgcgacc ggagctgatc ttggccaggt
     7681 attggtcatt ggtctgcata aacatttcct tatcggcgat acccatattc catagttcat
     7741 tgacgaattt taggaatgtt aaattgaaat cttgggtcat aatgaccttg gcttccagag
     7801 tctcctgatc cacacatgcg ggtccgtcat tgggatagcc atccaagaat cttgcagcac
     7861 cgtattgcag tccggacatc ctccaacctt ccccgggcag tgtaaaggcg attgtaggtt
     7921 tgccattata ttcaggattc ttctggacat agtcctttag aagatctgcg tactcatcca
     7981 atgtcttgat tacgggccat cccgcctttt ctacagcatc agctaccaga taaaaacctg
     8041 ccgtaggata cagggtgtcc tcacctgccc tgactccgct gatgtgatag gtagtgccat
     8101 actgcatctt taagagttct aattctgagg ggcgatatat ctgttttatg ttctctccat
     8161 actcttccag gtaatcatcc agtggaacaa acgcaccggc agccacataa tcacctgtgg
     8221 actcgcccgg aacgatgata tccggataat cgcctgaggc aatcataacg ccagcttttt
     8281 gcctggcatc ggtcccaacc aaatattctg tttttaattt aacgccggtt aattcggtaa
     8341 ttttcttacc aacgggtgtc tcccatggat cggtcatgga ggtcaatgca tagttaataa
     8401 aagctgtgta agtagtaacc tccccgggct tttcatcttg cggtgtttct tcttttccgt
     8461 tcccggattg ggggggatca gctttcccgg attgctcctt tgtctgacca catccggcga
     8521 gtacggcgat taccaaaata agggcaataa gcatcgtaat aaaccgtttg cacttcatta
     8581 aatctcctcc ttttgtttgt ctatgtcatt tgtacattta aaatatttct taattgtaca
     8641 aatcgcatac ttttgttcag tagactatta tttcatcaaa aggatcctgt ttggaataat
     8701 tactattggc tttacttgcg ctttaattat ataatccccg ttcaaatcaa acaacggggg
     8761 aaactatagt ttcccccgta aaaaaatata ggtttttctg tctaatccca cttaattgat
     8821 cgttttcccc tatggggctt cactcatcaa tcacctataa ggctcttctt gtattgcgag
     8881 ggggtcatat ttacttcctt tgcaaaccgg ctgacaaagt aatccgtatc cctatatcca
     8941 acatccgccg caatctcata aaccttcata tccgtccttc gaagcagctt cttggcttct
     9001 tccaccctta gattgtgaag ataatcactg aaatagatac cgaaggtctt cttaaagagt
     9061 tggcctagat ataccggatt cataaagaat tcttcggcga taccctttag tgtgatgtct
     9121 tcgcgaaagt tctgacgaat gtaatcctcc accttagcga tgggccccat ggattgcctt
     9181 tccctcagcg ttttaatata agcggcaccc ttaatgcaga accttgtcag aacctccttt
     9241 aatacatcaa tcgtgatctt atctatatcc atgttcagca cggtaaattc ttctaccagt
     9301 tcgtccatat tcccatccat ttgggtaatg atcttcgcta tctccagtgc aaagttattt
     9361 agataggcct gaatgatctc gggggccagg aaaagcttta taagctttat aaacagggca
     9421 tccacctggc tatatacttc atcggccttg tcgttctcaa tggcttccac caaggcagga
     9481 aaggacaaga cctccttaaa accataatta aaggtaattc cctttatctc atcatagaaa
     9541 ataatacagt ttccgcctct aaaaaaacgg tggtgcatag cggtgaggga ggatttgtgg
     9601 gatcgtccca aagccttcaa tcccttaacc tgctccccta cgaatacgga ggcgtctatc
     9661 tgcgcctcga gatttatctt ctccctgatc ctttgcgcta gctcctccat gttcccatgg
     9721 tattggtcca aaattttacg accggtaata attccatacc gaaaatcccc ttcttcgaac
     9781 aggtttcgcg cattttcctc ccctattata tccaccagca gctgtcttac atgaaatccc
     9841 ctattctcca gttccacggg atctaaacca cttagtctat ttttgtaatg atcctgtt
//
LOCUS       DASRNX010000091        13498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_706429,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000091 DASRNX010000000
VERSION     DASRNX010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13498
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706429"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFD89_03565"
     CDS             complement(<1..204)
                     /locus_tag="VFD89_03565"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HZJ57301.1"
                     /translation="MCTKGTIDRFEGDWAVVELENGEIVDMPMGVLPPDVEEGSIIYI
                     DEDGRINVSLQETDEQSNRVRKLM"
     gene            complement(319..1215)
                     /locus_tag="VFD89_03570"
     CDS             complement(319..1215)
                     /locus_tag="VFD89_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZJ57302.1"
                     /translation="MKIRRFKIGGILMVLLLAMALILTSCALLDELDEILDIIEGEDA
                     PIPEGVMAVHFIDVGQGDSILIRLPSGENMLIDAGDNDKGKVVVDYLKEQGIEKIDHL
                     VGTHPHADHIGGLDDVIDEFDIGDIYMPKVSHTTKTYMDVLEAIDRKGLKIKAAKSGS
                     TIPTEGAKAEILAPDSDLKSDNLNDYSAVIRLTYGQTTFLFQGDAEKKTEDLILSSHT
                     NIKAHVIKLGHHGSSTSNTPDYIETVDPRYAVITLGAGNKYGHPHKEITDLLDAKGIT
                     VYRTDQNGTVIAVSDGREVSFK"
     gene            1710..1925
                     /locus_tag="VFD89_03575"
                     /pseudo
     CDS             1710..1925
                     /locus_tag="VFD89_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749072.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
     gene            2003..2239
                     /locus_tag="VFD89_03580"
                     /pseudo
     CDS             2003..2239
                     /locus_tag="VFD89_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(2543..3034)
                     /locus_tag="VFD89_03585"
     CDS             complement(2543..3034)
                     /locus_tag="VFD89_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57303.1"
                     /translation="MDITKFKKVVFVVSILTLIGMVGVAYAASGHKRPADIVSELTGR
                     SVEELYEERDSGKTFGQIADEAGKLDEFRSEMLVEKKAVLDERVQEGRISQERADTIY
                     DAMKENQINCDGTGRHAGWDGNTTGQGFGHRHGNGHGLGMGMGMRRGHGAGYGIGHGR
                     GRK"
     gene            complement(3102..4511)
                     /locus_tag="VFD89_03590"
     CDS             complement(3102..4511)
                     /locus_tag="VFD89_03590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ57304.1"
                     /translation="MKSIRSKLWVGMMVLVALVLLLLWFSQIVFLERFYLHIRVSEIK
                     DRAYRTATKLIDGQSINMEDKLDALASDENINLEFLDPDGALIYSTGMTGSMRGGHMG
                     MMKGNAWKQILRRVLSGEEIVTAMTHMRLNSQFILMGLPIKSDVGIIGAMLINMPIAP
                     IDATVAILKRQLILITIVLVVVSVIISSFISKTLSKPIRDITNAAEGMARGDFDIRIK
                     TKSRDEIGVLANTFNHMGSELTRIDELRKELIANVSHDFRTPLSLIRGYAETIRDVTG
                     ESPEKRERQLGIIIDESQRLGSMVDDILNLSQMQAGYIILSMEEFDIHDTVEGIRKRF
                     EILCREHHIELDNKVHGSHLVLGDEFRIEQVLYNLISNAFAHTPSGGKVTISGSDLGE
                     RIRIEISDSGSGISEKELKDIWNRYYKGDRKGGRTLKGHGLGLAIVKSILDAHKLPFG
                     VKSREGEGTTFWFEMEKSF"
     gene            complement(4498..5202)
                     /locus_tag="VFD89_03595"
     CDS             complement(4498..5202)
                     /locus_tag="VFD89_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015592391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZJ57305.1"
                     /translation="MDRERILIVDDEELIRDIIKEYLGPEGYIVDEAGDGEEALDLFA
                     RNRYSFIILDIMMPKIDGWSVCREIRKTSDVPIIMLSARGEEYDKLFGFELGVDDYLV
                     KPFSPKELLARIKAIMRRSNSGHTNAGEPTKRVFQGLVVEFDSRNVSVDGGRINLTLK
                     EFDLLSLFVKNPNRVFTREQILNQVWGYDFFGDDRTVDTHIKMLRESLMGYRKFIVTV
                     WGIGYKFEPGESHEKH"
     gene            complement(5582..6763)
                     /locus_tag="VFD89_03600"
     CDS             complement(5582..6763)
                     /locus_tag="VFD89_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HZJ57306.1"
                     /translation="MQEYFVDLHVHIGRSSLGKITKRGTSNKLTFSNIAYESHHRKGI
                     HVIGVVDCVSPWIIRDIEELLHAGELNEIPGGGVVYSDDLVILLGSEIETRESHGCSA
                     HSLMFFPSLAQVKEFSRIMDNHMTNLEYNATVSRLSVQELFDIADSLEGIFIPAHIFT
                     PYKGLYGNCTDRLARIFNPSSLEKIPSIELGLSADTDMGTQISELDSKTFLSNSDAHS
                     LGRMGREYNTMLLGGLDFREVVKALRNQDGRRITANYGLDPKLGKYYRTFCMKCENIT
                     ELPPPITKCPNDPKHRVVMGVLDRLVTIRDREESPKDTNRPPYHYQIPLEFIPGVGPK
                     TIDRLIDAFGSEMNILHKASREEIREVVSEDITDMIVKGRGGELTITHGGGGKYGKVE
                     H"
     gene            complement(7001..8494)
                     /locus_tag="VFD89_03605"
     CDS             complement(7001..8494)
                     /locus_tag="VFD89_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZJ57307.1"
                     /translation="MDSRRTNFVFFFPDEMRASSVSCYGNQTVKMPNFDRLARDGVKF
                     DNCIVQNPVCTPSRCSLMTGLYVHNMGHRILWHLLRPHEPSLFRYLKDAGYDIGWYGK
                     NDLYSQEYLEEICSDIDEKRYGYRQETSNKAGRVQGGQNPYANDDPKYYSFLYDAIES
                     EGEDIPMEVDIARGIDFLKNRGEEDNPFMLFLPIGMPHPPYNTLETFHNMYDSDEVLG
                     DIIGVGEKSGAPSFMELIRKYRNLDKLDPKTFAKIYAVYLGMNSYVDLMLGQILETLD
                     KTGLADNTVVIVSSDHGDWAGNHGLVEKWPNAMDDDMVRVPLLIRSPDNKAGHVVKEQ
                     VELFDIMATVLEMAGIEARHTHFSQSLVPQILGAPGDPDRAVFCEGGYDLHEPNCFEG
                     FPRGEGAANLMDPENIYYPKALQQQEHPQSVCRTTMVRTLEHKLIKRTSGENELYDLK
                     NDPKELNNLYDDPKSHQVKLALEEKMLNWYIRTSDTVPLDDDPRGFK"
     gene            complement(8622..10361)
                     /locus_tag="VFD89_03610"
     CDS             complement(8622..10361)
                     /locus_tag="VFD89_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HZJ57308.1"
                     /translation="MATFTISGKQFLYDEEPIRILSGAIHYFRVVPEYWKDRLLKLKA
                     CGFNTVETYVPWNMHEPRPDEFDFEGMADIVKFIETAREVGLMVIVRPGPYICSEWEF
                     GGLPSWLLADPDMGLRFFNEPFLERVDKFFDALLPRLKPLLCTQGGPIIAMQVENEYG
                     SYGNDKRYLEHIRQGMVDRGMDVLLFTSDGPTDGMLQSGTLDDVFMTANFGSRPEEGF
                     GKLMEYQPDKPLVCMEYWNGWFDHWGTEHHTRDGEDVADVLDRMLKMGGSVNFYMFHG
                     GTNFGFYNGANRYEKYTPTVTSYDYDAPLSECGDPTEKYYAIQEVMSKYVSVDKDLMP
                     RPIAKRGYGRLELTEHASLFDNLHGISMPIQTVSPMPMERFGQDYGFILYRTTIQGPR
                     QDNQLFIQDVHDRALIFVDGELKGTYMRDEPQDMIFDIPECGVKLDILVENLGRINYG
                     PHLKDYKGITQGVRLGNQFLYDWTVYTLPLNDLSGISYKTNRGNEFPAFFRGKLDVDA
                     PADTFLSMAGWGKGIAFVNSFNLGRYWDIGPTKTLYIPAPLLKEGENEIVVFELEGCR
                     RAVVEFLDKPDLG"
     gene            complement(10427..10888)
                     /locus_tag="VFD89_03615"
     CDS             complement(10427..10888)
                     /locus_tag="VFD89_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019364889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZJ57309.1"
                     /translation="MIIGVISDTHGRITNRTLETLQGSELIIHAGDVGNLRVLDALED
                     IAPVYGVRGNTDRERWAQTLPMTRMVEAGGKFIYVIHDIAMLDIDPRAAGVDVIIYGH
                     SHIPKKDQKDGVIYFNPGSAGPKRFKLPICLGRIRLQEDELKFQWVDLEVK"
     gene            complement(11303..12124)
                     /locus_tag="VFD89_03620"
     CDS             complement(11303..12124)
                     /locus_tag="VFD89_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ57310.1"
                     /translation="MSIKNNPGFSIEVKGLNWRIGDAEILKDIRLQLDKNSFVGIIGP
                     NGSGKTTLLRNISTWYKPHGGTVLIHGRDVLSFTSRALAHEMAFVTQNANIEFEFTAM
                     DIVLMGRSPYIPRFGSETKEDIEKTEEVMWLTNTWEFRDRSVTTLSAGEMQRVMIARA
                     LVQDTPILLMDEPISNLDINHQIQIMDLIKGYQRKRGVTVVMVLHDLNIAAQYCQELV
                     LLDGGRIFCWGEPESVLTADNIRAIYGIDVHIMDNPLSGYPMIIPISTLWDHRPL"
     gene            complement(12146..13192)
                     /locus_tag="VFD89_03625"
     CDS             complement(12146..13192)
                     /locus_tag="VFD89_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HZJ57311.1"
                     /translation="MIYIHRRRHYRKVYVALLISLFIVILFSCSIGAANLTMADALRI
                     ILQKLPLIGEILVQEIPTDTMVSIVWKIRMPRVLLAGVVGAALAFSGGVYQGLFKNPM
                     AESFVLGVSAGAALGGAISMIFGVSRTVGIGGTGSMAFLGALLTTFLVYQLGKAGGRV
                     SMTSLLLAGMAINFMLSAAISLLMIFNRQRMDNIIMWTMGSFSNSTWEKVALAAVTIM
                     AGMLVTRFFARDLNILLMGEEEAKYLGIDVERTKKILLALSSFIAAMAVAVSGIIGFV
                     GLIIPHAIRLLMGPDHRVLLPYSAVVGAIFLIIADTLARTLVSPMEIPVGIITALAGA
                     PFFLYLLGKKRRTI"
BASE COUNT         3489 a         3479 c         2673 g         3857 t
ORIGIN      
        1 cattaatttt ctgaccctat tactctgctc atcggtctct tgcagtgata cgtttatcct
       61 gccatcctca tctatataga taatgctgcc ttcctccacg tcagggggta aaacccccat
      121 gggcatgtcc acaatttccc cgttttctaa ttccactacc gcccagtccc cctcaaacct
      181 atctatagtt cctttcgtac acatagcatg cctccacaat catttttccg ttaattatcc
      241 ggtttaaaca agcgtcggga acgttgtaaa acagacctat aattacaaga ttgactacag
      301 cctttagatt atggcttatt atttaaagga tacttccctg ccgtctgaca ccgcaataac
      361 ggttccgttt tgatctgtcc tgtagacagt aatccctttt gcatctaaga gatcggttat
      421 ttctttatgt gggtgcccat atttgttacc tgcccccagg gtaataaccg catatcgagg
      481 gtcaacggtc tctatgtaat ccggcgtatt tgaggtagaa gatccatgat gcccaagctt
      541 gatgacatgg gctttgatgt tggtatggga tgacaggatt aaatcctccg tcttcttctc
      601 tgcatcccct tgaaacagaa aagtcgtttg cccataggtc agacgaatga cggcagaata
      661 atcgttgagg ttatccgatt ttaaatcgct gtccggtgcc aaaatttctg cctttgctcc
      721 ctccgtgggt atcgtggagc ctgatttagc agccttgatc ttaaggccct ttctgtcaat
      781 ggcctccaat acatccatgt aggtcttggt agtatgggaa accttgggca tgtatatatc
      841 cccaatatca aattcatcga ttacatcatc aaggcctcct atatggtctg catgggggtg
      901 ggtccctacc aaatgatcta ttttttctat cccttgctcc tttaggtaat ccactactac
      961 ttttccttta tcattatccc ctgcatctat gagcatgttt tcaccggaag gcaaccttat
     1021 caggatagaa tccccctgcc cgacatcgat aaagtgtacc gccataaccc cttcgggaat
     1081 aggggcatct tctccttcaa tgatatccaa tatctcatcc agttcatcca ataatgcaca
     1141 agatgtcagg attaacgcca tagccagcag cagaaccatt aaaattccac cgattttaaa
     1201 tcttctaatt ttcatgtaaa cccccttgaa actccctata ggctattata ccataaatac
     1261 aatccctaat tgtatttatg cataacaata taacgaggtg gtaggtataa atctcccacc
     1321 tcgttgaaac ctgtgtgttg caattgccaa tgcaactgct tccggtgtta tgattttcca
     1381 cttttctgat ttattagcaa tcctatcgaa acccgaacca ttttggtgat ggaaatttcc
     1441 caaactatta aaaaaatcat gtcttcattt ggaacgaggg gggtaaaaca gatgcaaata
     1501 gttgacttta ccacagatca tattgaagcg gcggcgcagc ttgcaaagct gaactatgac
     1561 gaggagagga gctttgttcc catcctgccc cctgtagaca gggtgccgga cttaaagcca
     1621 tatgcctcta cgcccatgac agtgtattac aggagcaatt ctttcgatac ggctttggcc
     1681 tacgctgtgt tgatgcgata cggggaatgg acgagatacc agtatacaat gacgataatc
     1741 cccatgaata taaattctcc gggaaacgta tcaagcgcgg cctgtacagg actaaatcgg
     1801 gatatacctt caatgctgat gtaaacgggg cattaaacat tttaagtaaa agtagcattg
     1861 tgtccttaac agggctatac agtagaggcg aactggacac gcctgtacgc atcaaaatag
     1921 cttaggctac agaggcgtaa atgagttcat ttgaactcta gaaccccgtg actttagtca
     1981 tgggaggttc agacaagaga ctccgggata cctgcatgta aaaggcgcct attgtctgcc
     2041 ggaacaccgg ggcaagggtc taaaccagac gctactgaat atgctgatac gaaagctcaa
     2101 gataatgggc tatacaaggc ttggcgtgga ttttgaaagc ataaacccgg cggcatgggg
     2161 gttctggctt aaatattttg acgcctatac taatagtgtt gttggcagga tcgatgaaag
     2221 ggcagtaata tcagtataac aagcctctgg acaccgaatg gttttaatac aattttctac
     2281 caccgtatat agcccgaggg gttagtatta caactgctta cggtatgatt ttctaatgaa
     2341 atagagtttt gacatcctaa tcgcaacaat agataaagtg accggttata tccaataact
     2401 tacatttgaa cataaccggt cactctttca tcgattaagg gtttccgcca aaaagtcact
     2461 tgttatcata agtgcctgag ttgcacaccc taacagctat aattatagca tgcgcgcatc
     2521 tttagatatg gagggtacaa catcatttcc tgcccctgcc atgccctatt ccgtaacccg
     2581 ctccatgtcc ccttctcatt cccattccca tccccaaacc atgcccgttc ccatgcctat
     2641 ggccaaagcc ttgtccggtt gtatttccgt cccatccggc atgcctgccc gtaccgtcac
     2701 agtttatctg attttccttc atagcatcat atatggtatc tgcccgctcc tgagatatcc
     2761 gtccttcttg taccctttca tcaagtacgg cctttttctc taccagcatc tcgcttctaa
     2821 actcgtctag ctttcctgct tcgtcggcta tttggccaaa ggttttgccg ctgtccctct
     2881 cttcatatag ctcctcaacg gatcttcctg taagttcgga tactatatcc gccggccttt
     2941 tatggcctga agcagcatag gcaacgccga ccatcccaat caacgtaagt atcgatacaa
     3001 caaaaactac ttttttaaat ttcgtgatat ccatccgctc cttttctatt gatttgtagt
     3061 tttagtataa ccggcaaatg tgatagaagt gtgatagaag tttaaaagct tttctccatc
     3121 tcaaaccaaa atgtcgttcc ttcgccttcc ctgcttttga caccaaaggg aagcttatgt
     3181 gcatctaata tgctctttac tatggcaagg cccagtccat gcccttttag ggtgcggccc
     3241 ccctttctgt cgcccttata atatcggttc caaatatctt ttaactcctt ctcggagatg
     3301 ccggacccac tatcacttat ttctatcctt atcctctcac ccaaatctga accggagatt
     3361 gtaaccttgc ccccacttgg ggtatgggcg aaggcgttac ttattaggtt gtataacacc
     3421 tgttcaatcc taaattcatc ccccaatacc aagtgacttc cgtgtacttt gttatcaagc
     3481 tctatatgat gttccctaca aaggatctca aacctttttc taatcccctc aaccgtatca
     3541 tggatatcaa attcctccat agaaagaatt atatacccgg cctgcatctg ggataagttt
     3601 aatatatcgt ctaccatact gcccaggcgc tgtgattcgt ctataattat gcccagctgc
     3661 ctttcgcgtt tttcggggct ttcccccgtc acatccctga tggtctcggc ataacccctg
     3721 atcaggctta gtggggtacg aaaatcatgg gacacgtttg ctattaattc ctttctgagt
     3781 tcatctatcc gggtcagctc cgaccccata tggttgaagg tattggccaa tacaccgatc
     3841 tcgtccctgc ttttggtctt tatacgtata tcaaaatccc cacgggccat gccctcggct
     3901 gcattagtga tgtctcttat gggttttgac agggtcttgg atataaaaga tgatatgata
     3961 accgagacta caacaagtac aatggttata agaatcaatt gtctttttaa tatagcaacg
     4021 gttgcatcga taggcgcaat gggcatgtta ataagcatgg ctccaataat acccacatca
     4081 ctctttatag gcagacccat taaaataaat tggctgttaa gtctcatgtg tgtcatggcc
     4141 gtcacgatct cttcaccgga taggacccga cgaaggatct gcttccatgc attgcccttc
     4201 atcataccca tatgcccgcc tctcatactc ccggtcatac ccgtggaata aatcaaggcc
     4261 ccatccggat ccaaaaattc taagttgatg ttttcatcgc tcgccagtgc atcgagttta
     4321 tcctccatat ttatgctctg accgtctata agcttggttg ctgtcctata ggccctgtcc
     4381 ttaatttcac ttacccttat gtgtaaataa aacctctcca aaaatactat ttgggaaaac
     4441 caaagcaata ataataccag agcaacgagg accatcatac cgacccatag ctttgaccta
     4501 atgcttttca tggctttccc ccggttcgaa cttatagcct ataccccaga cggttactat
     4561 aaacttcctg tagcccataa ggctttccct taacatcttt atatgggtgt ccacggtcct
     4621 gtcatcgccg aaaaaatcgt agccccatac ttgatttagg atctgttccc tggtgaaaac
     4681 cctgttgggg tttttaacaa agagacttag cagatcaaat tcctttaggg tcaaatttat
     4741 tctcccacca tccacggaaa catttcggga atcgaattct acgacgagcc cttgaaacac
     4801 cctttttgta ggctcaccgg cattcgtatg tccactgtta cttctcctca ttatggcttt
     4861 tatccgggca agaagttcct ttgggctaaa gggcttaacc aaataatcat caacgcccaa
     4921 ttcgaagcca aagagcttat catactcttc ccctctggcg gacagcatta tgataggcac
     4981 atcggaggtt tttcggatct ccctgcatac actccaccca tcgattttgg gcatcatgat
     5041 atcgaggatt ataaaggagt acctattcct tgcaaacaga tccagggcct cctcgccatc
     5101 gccggcttcg tctactatgt atccttcggg cccaaggtat tctttgataa tatcccttat
     5161 aagctcttca tcatcaacta ttaaaatcct ttccctatcc ataatatcca accccatcgt
     5221 tatttaatag gcacctgaaa catgggcaaa aatccgaaaa agtgatttgt ataaccaccc
     5281 acatgcacgg aacccgacct cctatattaa atatataacg ggtagacaga aacttcaaac
     5341 aataagatta ggaagtatag tccccgccca ctataacgtt tttttgatca ggtatcatta
     5401 tgttgtatag ggattgattt ttgtgtggga actgaaattg attcgatcta atactcttta
     5461 atctaatgtt ccaatgaaat gacaccttta tcaggatcag ttaaaggttc ccgaaagacg
     5521 acaggattcg ggaactttta taatgtgaag atagcggaca caggatgtgc cattaatcgg
     5581 cttaatgttc gaccttcccg tactttcccc cacccccatg ggtaatggtt aactcacccc
     5641 cacgaccttt cacgatcata tcagtgatat cttcggatac cacctctcga atctcttctc
     5701 gagaagcctt gtggaggatg ttcatttcgc tgccaaaagc atcaataagc ctgtctatgg
     5761 tcttaggtcc tacccccggg atgaattcaa ggggtatttg ataatggtat gggggacggt
     5821 tagtatcctt tgggctttcc tcccggtccc gaatcgtcac caatctgtct aaaactccca
     5881 taacaaccct atgctttgga tcgttggggc atttcgtaat cgggggcggt agctccgtaa
     5941 tgttctcaca tttcatgcaa aaagttctgt agtacttacc cagctttggg tccaggccgt
     6001 agttggctgt tatccttctt ccgtcttggt tcctcaaggc cttgactacc tcgcgaaaat
     6061 ccaggccacc taaaagcatg gtgttgtact ccctacccat tcgccccagg gaatgggcat
     6121 cggaattact aagaaatgtt ttagaatcaa gctccgatat ctgagttccc atatcggtat
     6181 ctgcactcag gcccaattcg atggagggga tcttttccag agaagacgga ttaaaaatac
     6241 gtgcaaggcg atccgtacaa tttccataga ggcccttata cggggtaaag atgtgggcgg
     6301 gaataaagat cccttccaat gaatccgcta tgtcaaacaa ctcctgaact gataacctgc
     6361 taactgtggc attatattcc aagttggtca tatggttgtc cataatccgg gagaattctt
     6421 tgacctgggc cagggacggg aagaacatca aagaatgggc gctgcagccg tggctctccc
     6481 ttgtctcaat ctccgagccc agcaaaatca ccaggtcatc gctgtagact accccaccgc
     6541 ccggaatctc atttagttcc ccagcatgga gcaattcttc aatatctctg ataatccagg
     6601 gggaaacaca atccaccacc cctatgacat gaattccctt tctatgatgg gattcgtagg
     6661 ctatattact aaaggtcaat ttattggatg tcccacgttt ggtaatcttc cccaaagatg
     6721 atctgcctat atggacatga agatccacaa aatattcctg catcagctta ctcctttgac
     6781 atctattcta tgggcagtaa ctacagataa aaatcttcaa ccatagaatc tttcatctgt
     6841 agataaattt agtttccgcg ttgcctatct atatatcccc attagtatcc tacagataaa
     6901 cattatcata ctaaaaacta tccagactta ttaaatgtcg aagagattgg cgaccaacta
     6961 tacagatacc aacccaatcc atgcctattc gtcttatccc ttacttaaat cccctgggat
     7021 catcgtcaag aggcactgtg tcggaggtac ggatatacca gtttaacatt ttttcttcca
     7081 gggccagctt cacttgatgg cttttgggat cgtcgtatag attgtttaat tccttaggat
     7141 cattttttag atcatatagt tcattttctc cggaggtacg cttgataagc ttatgctcca
     7201 aggtgcgcac catggtggtt cggcacacgc tctgagggtg ttcctgctgc tgtaacgctt
     7261 tggggtaata gatgttttca ggatccatca ggttggcagc gccttcgcct ctggggaacc
     7321 cttcaaaaca attgggttcg tgtaaatcgt agcctccctc acagaagacg gccctgtcag
     7381 gatcgccggg tgcgcctagg atctgtggga caagggactg ggaaaaatgg gtatgccgag
     7441 cctcaatccc tgccatctcc aggaccgtgg ccatgatatc gaagagctct acctgttctt
     7501 tcacaacatg ccctgcctta ttatcgggtg accttataag tagggggaca cgcaccatat
     7561 cgtcatccat agcattgggc catttttcta caaggccatg gttgcccgcc caatccccat
     7621 gatccgagga aacgatgacc actgtattgt ccgctagccc ggttttgtct agggtttcta
     7681 atatctgtcc cagcattaaa tctacatagc tgttcattcc taaatagacg gcgtatatct
     7741 tggcgaaggt ctttggatcc agtttatcaa ggtttcggta tttacgtatc agttccataa
     7801 atgagggtgc tcccgacttt tcccccacgc ctataatgtc tcccagcacc tcatcagaat
     7861 cgtacatatt atggaacgtt tctaaggtgt tgtatggggg atggggcatg cctatgggca
     7921 aaaacagcat aaaggggttg tcttcttcac ccctgttttt aaggaagtcg attccccttg
     7981 ctatatccac ttccatgggg atatcctccc cttccgattc aattgcgtcg tacaggaaag
     8041 aatagtattt agggtcgtca ttggcatagg ggttctgtcc cccttggaca cgccctgctt
     8101 tgttggacgt ctcctgcctg tagccatatc gcttctcgtc tatatcgctg catatctcct
     8161 ctaagtattc ctgggagtac agatcgtttt tgccatacca gccgatatca tatccggcat
     8221 cctttaggta tctgaacagg ctgggttcat ggggccttaa caggtgccac aggattctat
     8281 ggcccatatt atgtacatac aatcccgtca taagggagca cctggagggt gtacaaacgg
     8341 gattttgtac tatgcaatta tcaaacttga ccccatccct ggccaatcta tcgaagttgg
     8401 gcatttttac cgtttgattt ccataacatg agacactcga agcccgcatc tcatcgggaa
     8461 agaaaaagac gaaattagtt ctcctgctat ccatataacg actcctttca aagtccttta
     8521 tcccataacc taaacatgag actcttcatc aatgcgggat aaaaactatc ccgagatttt
     8581 tttgtggcgc agtaaggtaa cataataggt caggtatacg tctagcccag atcgggttta
     8641 tccaaaaact ccacaacagc tctgcggcag ccctctagtt caaagactac gatctcgttt
     8701 tccccttcct ttagcaaggg tgcgggaatg tataaggtct tggtgggccc aatatcccaa
     8761 tagcgaccca ggttaaagct gttaacaaat gctatgccct taccccaacc cgccatagat
     8821 aaaaaagtat ccgccggggc atccacgtcc aattttcccc taaaaaatgc cggaaattca
     8881 ttgcccctgt ttgttttata ggaaataccc gaaagatcat taaggggcag ggtatatacg
     8941 gtccaatcgt ataaaaactg gttcccgagc cttacgccct gggtgatgcc tttataatcc
     9001 ttaaggtggg gcccataatt aattctcccc aggttttcca caaggatgtc cagtttgacg
     9061 ccacattctg gtatatcaaa gatcatatct tggggttcat ccctcatata tgtgcccttt
     9121 aattccccgt ctacaaagat caaagcccta tcgtgtacat cctgtatgaa taattggtta
     9181 tcctgcctcg gtccctgaat ggtggttctg tagagaataa acccatagtc ctgtccgaac
     9241 ctttccatgg gcatcgggga taccgtttga atgggcatgc ttatcccgtg cagattatca
     9301 aacaatgatg catgctctgt cagctccaat cgtccatagc ccctcttagc tatgggtcgg
     9361 ggcatcaaat ccttatctac acttacgtat tttgacatta cttcttggat tgcataatat
     9421 ttttctgtgg gatcaccgca ctcgctaagg ggtgcatcgt aatcataact ggtaaccgtc
     9481 ggagtatatt tttcgtacct attggccccg ttatagaagc caaagttggt cccgccatgg
     9541 aacatataga agtttacaga gccgcccatc ttaagcatcc tgtctaggac gtctgccaca
     9601 tcttctccgt ctctggtatg gtgctctgtc ccccaatgat caaaccagcc gttccagtat
     9661 tccatgcaca ccaagggctt atccggttga tattccataa gcttcccaaa gccctcctca
     9721 ggccgtgacc caaaattggc ggtcataaat acgtcatcca atgtcccgga ttgtagcatc
     9781 ccatccgtcg gtccgtcgga ggtaaaaagc aaaacatcca tacctctatc taccattcct
     9841 tgccttatat gctctaggta ccttttatcg ttaccatagc tgccatattc gttctctacc
     9901 tgcatagcaa ttatgggccc gccctgggtg caaagcagtg gtttcaagcg gggcaataga
     9961 gcatcaaaaa acttatctac ccgctctaaa aagggttcat taaaaaacct caatcccata
    10021 tccggatccg ccaagagcca tgaaggcaga ccgccgaatt cccattcaga gcatatgtaa
    10081 ggcccaggac gtacgattac cataagcccg acctcccggg ctgtttctat aaactttaca
    10141 atatccgcca ttccctcaaa atcgaattcg tcaggcctag gctcatgcat gttccatggt
    10201 acatatgttt ccacggtatt gaatccgcac gcctttaatt ttaggagcct gtccttccaa
    10261 tactccggca ctacccgaaa atagtgtatt gcccccgata ggattcttat gggttcctcg
    10321 tcataaagaa attgtttgcc tgaaatcgta aaagtggcca ttattcttcc ccctccttgg
    10381 attttattaa tacgaagggt cttttcctca atacatttaa caaagcctat ttgacctcca
    10441 aatccaccca ttgaaatttc aattcgtctt cttgtaatct tatccgcccc aagcaaatgg
    10501 gcaatttgaa ccttttaggc cctgcgcttc ccgggttgaa gtatatgacc ccatcctttt
    10561 ggtccttctt ggggatgtgg gaatgtccgt atatgatcac atcgactcct gctgctctcg
    10621 gatcgatgtc cagcatagcg atgtcgtgta taacatagat gaacttaccc ccggcttcga
    10681 ccatccgggt cattggaagg gtctgtgccc acctttccct atccgtgttt ccccttaccc
    10741 catagacagg agcaatgtct tctagggcgt ctaggaccct tagatttcct acatctcccg
    10801 catgtatgat gagttcgctt ccctgtagcg tttctaaggt gcggtttgta atccttccat
    10861 gggtatccga tatgactccg ataatcattt atttctcctc ttttgctgtg cgtgatggtt
    10921 agacccccca tattgggtta gtttggtata tatcggtaat ggctattgaa cgctacttcc
    10981 gacattgatt tggagaaaac taaggcttat tccatttgaa aagcctatcg cttccaatcc
    11041 ggcctcctgc cggtggaatc ggcttaaggc gtcctgcctt aatctacggc ttttctgcac
    11101 tccataagcc aagttttctt accaaatcaa tgaagtcatc cgctccccaa tagtccatta
    11161 cctagataat gggctaacct gatagattgg ccccgtaaaa tattataagc taatcaaagc
    11221 ccatttacaa acatctcctt gtccccaagt tcagtgtcat ttaagcatat ccggagaatc
    11281 cgacaccgtc ttttctatct ccctacaagg ggcggtgatc ccaaagggtt gatatgggta
    11341 ttatcatggg gtatccgctt aggggattgt ccatgatatg gacatcaatt ccatagatcg
    11401 cccttatatt atccgcagtc aatactgatt caggctctcc ccagcagaag atccttcccc
    11461 cgtccaataa tacaagctcc tgacaatact gtgcggctat gttaagatca tggagcacca
    11521 tgaccacagt cacgcccctt tttcgttggt agcccttaat cagatccatt atctggatct
    11581 ggtggtttat atctagattt gatatgggct catccattag caaaataggg gtatcctgaa
    11641 ccagggccct ggctatcatg accctttgca tctcaccggc actcaaggta gtgactgatc
    11701 tatccctgaa ctcccacgtg tttgtaagcc acataacctc ctcggttttt tcgatatcct
    11761 ccttggtctc acttccaaac cttggtatat agggcgatct gcccatcaac actatgtcca
    11821 tggcagtaaa ttcaaactct atattggcat tttgtgttac gaaggccatc tcatgggcca
    11881 acgccctgct ggtaaaggat agtacgtccc ttccgtggat caagactgta cccccatggg
    11941 gcttgtacca ggtgctaata tttctaagaa gggttgtctt tccggaaccg ttagggccta
    12001 ttattcccac aaagctgttt ttatccaatt gcagtcttat atcctttaaa atctcggcat
    12061 cgcctatccg ccaattgagc ccctttacct cgatggaaaa tccggggtta ttctttattg
    12121 acatggtagc tcccccctcc catggctata tagtccttct cttcttgccc aacaggtaca
    12181 aaaaaaacgg agcacccgct aaagccgtaa tgattcccac gggaatttcc atggggctta
    12241 ccaaggttct ggctaaggta tcggcaatga ttaaaaagat ggctcctacc accgctgaat
    12301 agggcaatag caccctatga tccggtccca tcaggagcct tatggcatgg ggtataatca
    12361 gaccgacaaa cccgatgatt ccgctcacag ccaccgccat tgcagcaata aaggaggata
    12421 gggccagcaa tatctttttc gtcctctcca catcaatacc cagatacttg gcctcctcct
    12481 cccccatgag cagaatattc aaatcccttg caaaaaaacg ggtcaccagc atccccgcca
    12541 taattgtgac tgcggccaag gccaccttct cccaggtaga attggaaaag ctccccatgg
    12601 tccacatgat aatgttatcc attctttgcc ggttgaagat cataagcaag gatatggccg
    12661 cagacagcat gaagttaatg gccattcccg ccagaaggag ggaggtcatg gataccctgc
    12721 cccccgcctt tcccagttga tagaccaaaa aggtagttag aagggctcct aaaaaagcca
    12781 tagatcctgt gccgccaatc cccactgtcc gagacacccc aaagatcatg cttatggccc
    12841 cgcccaacgc ggcgcctgcc gatacaccaa gaacaaagga ttctgccatg gggtttttaa
    12901 acaaaccctg atagactccg ccggagaatg ctagagcagc gcctacaacg ccggctaaaa
    12961 gaaccctggg catccttatc ttccacacta tggaaaccat agtgtctgtg gggatctcct
    13021 gtactagaat ctccccaata aggggtagct tttgtaggat gatcctcaac gcatccgcca
    13081 tggttaaatt tgcggcgcct atggagcagc tgaatagaat aacaataaac agcgatatga
    13141 gcaaggcaac atagaccttc ctataatgcc ttctcctatg gatataaatc atatgtccta
    13201 ccccctatat tttaaccccc cgaaggcgaa atctaaaagg cgcatcaaac aacatgtcac
    13261 tttaggcaaa gccaaagacc tcggaagttt ccaggcaatg cccagaatga caaaggtgtt
    13321 ttaatgcgcc atatttacaa cactaccggg gtatgaaacc ggtataacga tttaaaatcc
    13381 taatcaaaca ggtcagggtg catgcccttt gccaggtctt ccaaaccctc tattagtcta
    13441 gggccggctc tgtttatgag gttgtcgtcc agcacgatca accggccctc ttttatag
//
LOCUS       DASRNX010000092         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_231975,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000092 DASRNX010000000
VERSION     DASRNX010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231975"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..893
                     /locus_tag="VFD89_03630"
     CDS             336..893
                     /locus_tag="VFD89_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZJ57312.1"
                     /translation="MEDQEIIDLYWNRKETAIRETMNKYGRFCYSIAVNILYIPEDAE
                     ECVSDTYHKVWNSIPPERPIAFRGWLGRIVRNLSINRWHYNRAQKRYGGIEIMLSELS
                     DCIPDTITTEEIIETHVLSQYISNWLDTLSDTERRLFVLRYWNGEKLKHLATSYRMTP
                     NKLAGKMYRLRISLKTYLTERGVAI"
     gene            890..>2334
                     /locus_tag="VFD89_03635"
     CDS             890..>2334
                     /locus_tag="VFD89_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57313.1"
                     /translation="MKREVKLFNAITEIRDDLIEEAQETKKRFYRPNWKRWGTIAAGI
                     ILALGIGTFALFNNWFPFGGSSSPGSEGGSGHLIGATTFMSYAGPVFPLTLENSEDNI
                     TAIRDISYDFALASEDSLRVWGSGITDSYTLENSSDEDMIIEGIYPFAGSFNGLVEEM
                     PTIKVNGEMITPNLYPGGYSGGFIGIMGADDPEGSSNILELDSWEGYKKLLEDGNYMR
                     RAFEDYPILSQKVTVYKFTDFKAPLEEFDAATQAISFTIDPEKTNILLYGFNGGEYNE
                     DGFRRYSYFVPNGIRRETDEKYIIIVGEDIENYVLQGYKNGACEEGNELEGVSATVTR
                     AEASLSDVFSEIVDNYFSTYDADEAIPVSRDMFLGIMSEFMLEHGVFSSFPKERYDYG
                     MLEDIISETKNLDRVFYLRFPINIPAGKDASVMVSLRKKPSYDFYGSGSENVGVQGYD
                     MVTRLGSNIGFTEITASLRNTDNIEILNQNFG"
BASE COUNT          737 a          414 c          544 g          639 t
ORIGIN      
        1 tatttgatgc gtttattgaa tcggtgtgaa gcaagccaat tgaggcttgc acttatgata
       61 tagcatggat aaaaatttga ggtgcaaaga tatgcgatca tggaatcaaa catcagggca
      121 tcagtagatt taggaaacaa atatactgga atatctatgc tatgcctgga tcggatatct
      181 tatctgagat taaccataag cacccctgca agccaagaca tatccgtaat ggtttttcat
      241 ttcatgatat aattaaaaaa gtaaacaaat atctaatggt tttctctgtc tatataagtg
      301 aggggactct atttcgcatg tgtaggaggg caaaagtgga agatcaagag attatcgatc
      361 tttattggaa tcgaaaggaa acagcgatcc gtgaaacaat gaataaatat ggtaggtttt
      421 gttacagcat tgctgtaaat atcctatata tcccggaaga tgccgaagaa tgtgtaagcg
      481 atacttatca taaggtatgg aatagcattc cgccggaaag gcctatagct tttcgggggt
      541 ggttagggag aattgttagg aatctttcta tcaatcgctg gcattacaat cgagcacaaa
      601 aacgctatgg cggtatagaa attatgttgt cagaactttc cgactgcatt cccgatacta
      661 taactacaga ggagataata gagacacatg tcctttctca atacataagt aactggcttg
      721 ataccctatc tgatacagaa aggcgcctct ttgtactcag atattggaac ggcgaaaaac
      781 tcaaacattt agcaacaagt tataggatga ctccaaataa gctggccggt aagatgtatc
      841 gactcagaat aagccttaaa acctatctga cagagagagg agtagccata tgaaaaggga
      901 agtaaaatta ttcaatgcca taacggagat tcgggacgac ctgatagaag aggcccaaga
      961 aacaaagaag agattctaca gaccaaactg gaagagatgg ggcaccatag ctgccggtat
     1021 cattttggcg ctgggaatcg gaacatttgc tctatttaac aattggtttc cttttggcgg
     1081 cagctcgagt ccaggaagtg aaggaggcag tggccactta ataggtgcaa ctacttttat
     1141 gtcctatgcc gggcctgttt tccctttgac tttggaaaac tcagaggata atattactgc
     1201 cataagggat atctcatacg actttgcctt agcaagcgag gattcccttc gcgtatgggg
     1261 cagcggtatt acagacagtt atacgcttga gaactcatct gatgaagata tgattataga
     1321 ggggatttat ccctttgccg gtagttttaa cgggcttgta gaagaaatgc caaccatcaa
     1381 ggtgaatggt gagatgatca ctccgaacct atatcccggg ggatattcag gtggttttat
     1441 cggaattatg ggggcagatg atcccgaggg cagctctaat attttagaat tggattcatg
     1501 ggaaggctat aaaaaacttt tagaagacgg gaattacatg agaagggctt ttgaagatta
     1561 tcccatcctc tcccaaaagg tgacggtata taaatttaca gactttaagg cgcccttaga
     1621 agaatttgac gctgcaaccc aggctatctc ctttacaatt gaccctgaaa agaccaatat
     1681 tttgctatac ggctttaatg gcggtgaata taacgaagat ggctttagaa gatatagcta
     1741 cttcgtacca aatggaattc ggcgagagac cgatgaaaaa tatattatca tagttggaga
     1801 agacattgaa aattatgtac tgcaaggtta taaaaatggg gcttgtgaag aggggaatga
     1861 acttgaaggc gtatctgcaa ctgttacacg ggcagaggca agcctatctg atgtattctc
     1921 agagattgta gataactact tttcgacata cgatgctgat gaggcaatcc cggtgtccag
     1981 agatatgttt ttaggtataa tgtccgagtt tatgctggag catggggtat tttcaagttt
     2041 ccccaaggaa cgttatgact atgggatgct ggaggatatt atctctgaaa ctaaaaacct
     2101 agaccgagta ttttatctaa ggttccccat aaatatcccc gcaggcaaag atgcttccgt
     2161 aatggtatcc ctaagaaaga aaccgagtta tgacttttat ggttccggtt ctgaaaacgt
     2221 aggcgtacaa ggctacgata tggtaacccg cttggggagc aatatagggt ttaccgaaat
     2281 aaccgcttct cttcgcaata ccgacaatat tgagatatta aatcagaact ttgg
//
LOCUS       DASRNX010000093         5332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_336772,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000093 DASRNX010000000
VERSION     DASRNX010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336772"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFD89_03640"
     CDS             complement(<1..647)
                     /locus_tag="VFD89_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZJ57314.1"
                     /translation="MEASSKKSLYKWVLYLAAPIALQNIIAMSVGLADNLMVGSLGEH
                     ALSGVFAANQLQNLLHMLVIGLGTALTVLAIQYWGKRDLESIKNLIGIALKFSIAAGL
                     LFLLATLVFPNQIIGIFSNVPEVKKEALDYLKIIRFTYLFFSVTQILIASMRCVETVK
                     VGMYLSIVTFFVNVFFNWVLIFGNLGAPALGVKGAAIATLIARVIEVPLMILYIR"
     gene            1056..2243
                     /locus_tag="VFD89_03645"
     CDS             1056..2243
                     /locus_tag="VFD89_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HZJ57315.1"
                     /translation="MVRTVGTTVRGIITPIIKEGDDLVRIVVDSVLRSMKSENYSLRD
                     MDVLGITESLVARAQGNFIHLDDIAADVNRLFTGDIGLIFPILSRNRFLPILKAMAMT
                     KKKIYIFLNYPSDELGNHLMQVEKMSETGINPYTDLLTEEQYRKLFGDVVLHPFTGID
                     YVALYKSLAIDDNIEIYLTNDPRPALDYAKEFLVANIHDRFRTKEILLNSGAEKVLCL
                     DDILTEPVSGKGYNAEYGLLGSNMATETSIKLFPRECNKIVMEIQETLKRETGKHIEV
                     MVYGDGAFKDPQGKIWELADPVVSPGYTPGLEGIPNEIKLKYIADSESGNIQPEDMAD
                     LVKSRIKSKDHDLMGKAESSGTTPRQLTDLLGSLCDLTSGSGDKGTPIVLVQGYFDNY
                     ASE"
     gene            2470..3657
                     /locus_tag="VFD89_03650"
     CDS             2470..3657
                     /locus_tag="VFD89_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HZJ57316.1"
                     /translation="MGFTINDKVTWVGKIDWELRNFHGYEYSVDKGSSYNSYLVRDEK
                     TVLIDTVWKPFAKEFVENLKKEIDLKEIDFIVVNHAEIDHSGALPELMREIPDTPIYC
                     TANGIKSIKGHFHEDWNFIPVKTGDTLNIGQNDLMFVEARMLHWPDTMFTYMTNDNIL
                     FSNDAFGQHYASEYMYNDLVNPDELYSEAIKYYANILNPFSVLVQRKIEEVIKLDIPL
                     DMICPSHGIIWRKDPLQIVEQYMSWAQAYQEDQISIIYDTMWEGTRRMAEALAEGIKE
                     GDDRVNIKLYNTSKTDKNDVMTEIFKSKAILLGSPTVNGSILTSMAAIVDLAKGLKFK
                     DKKAAAFGTYGWSGESVKVLTQHLENAGFEVVNQGIREMWNPDEEGLDRCREFGRNFV
                     QEL"
     gene            complement(3774..4640)
                     /locus_tag="VFD89_03655"
     CDS             complement(3774..4640)
                     /locus_tag="VFD89_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZJ57317.1"
                     /translation="MQPIIITDSCSDLPLEYVKKRNLPILSFTYNFMGVDRKDDLGQT
                     MSYRDFYDGVKGGQMPTTSQVNTQTYIDFFRQYVEQGQPIIYMCFSSALSGSFNSAVL
                     ARQMLLEEYPDAAISIIDTRCASMGLGLLVHMALDLRDQGLGYHQIVEWIENNKLRLV
                     HWFTVDDLEHLRRGGRVSGAAAFIGSILSIKPILHVDDEGRLIPVTKVKGRKKSLKGL
                     LEKMQETAINPEEQTVFISHGDSLEDANYLADMIRDQLGVKDFLINYVGPVIGSHSGP
                     GTIALFFLGTHR"
     gene            complement(4907..>5332)
                     /locus_tag="VFD89_03660"
     CDS             complement(4907..>5332)
                     /locus_tag="VFD89_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZJ57318.1"
                     /translation="YIVAESGAVLGTVHTLKENHRTNYIGTDIGFNVLARPMLYDSHH
                     DIEIYKKYGVPEENFRKATVVGNICESGDILAKDRLLPEIAEGDIIGIMDAGAYGYCM
                     SSNYNSRLRPAEVLITAEGRDVLIRRRDTLDDLMRNYIL"
BASE COUNT         1597 a         1086 c         1205 g         1444 t
ORIGIN      
        1 aatctgatat acaagatcat gaggggtact tcaataaccc gagcgattag ggtggcaatg
       61 gcggcccctt ttacacccag cgcgggggca cccagattac caaatatgag tacccaatta
      121 aagaagacgt taacgaaaaa agtaacgatg gataagtaca ttccaacctt aacggtctcc
      181 acgcaccgca tagatgcaat taatatctgg gttactgaga aaaagaggta ggtaaagcgt
      241 ataatcttca aataatccag ggcttccttc ttgacctcgg gtacattgct gaaaatcccg
      301 attatctggt tgggaaatac cagagtcgcc aacaggaaaa gcagaccggc ggctatacta
      361 aactttaggg caataccgat caggttttta atgctctcca ggtccctttt accccaatat
      421 tgaattgcca acacagtgag ggccgtacca agacctataa ccaacatatg gaggaggttt
      481 tgaagttgat ttgccgcaaa cacccccgat aatgcgtgct cacctaaaga accgaccata
      541 aggttgtcgg ccaatccaac ggacattgct atgatgttct gcagtgcgat gggagctgca
      601 aggtataaga cccacttata taaacttttt ttcgatgatg cctccatata actgtccctt
      661 ctttccaccg ttaatatatg agcctatgat acatatatat acgtataatg tcaatgcatt
      721 aacttttgat ggaacactgc cgaccccatg ccatgacctg aatatagcct tgggacttaa
      781 gcgattggat tattattctt cgattttaga ttatacaccc cttaaaactt gcgggactgt
      841 acacctatta tcagctatgg tataataatt ttgttggaga taaagatgaa ttatattcaa
      901 ggagtattaa agatacattc tattatgggc tgatcaagat atcggtgatc cgcctttttg
      961 aagtctcgat tcatgtgttg caattgccaa tgcaattgct tccgatgttt taattgatgg
     1021 gacattaata aacatttaga gagtgaggaa tacttatggt acgaacggta ggcactacag
     1081 taaggggtat aataaccccc ataatcaaag agggcgatga tttagtacgt attgtagttg
     1141 attctgttct aagatccatg aaaagtgaaa attatagtct tagggatatg gatgttttgg
     1201 ggattaccga atccctggtt gcgcgggcac agggtaactt tatacacctt gatgatattg
     1261 ccgctgatgt gaacagatta tttaccgggg atatcggcct tatatttcct attctaagcc
     1321 gtaatagatt ccttcctata ctcaaggcta tggctatgac gaaaaagaaa atttacatct
     1381 tcttaaacta cccatcggat gagcttggaa atcatctgat gcaggtagaa aagatgagtg
     1441 aaacggggat taacccctat acggacctgt tgaccgagga gcaatacaga aaattgtttg
     1501 gggatgtggt gttgcacccg ttcacgggga tagattatgt agctttgtat aagagccttg
     1561 ccatagatga taatattgaa atatatctaa ccaacgatcc caggccggcc ctggattatg
     1621 ctaaggaatt cttggttgcc aatatacacg acaggtttcg aaccaaagaa attttattaa
     1681 attcaggagc cgaaaaagtc ctatgcctag acgacatact gactgaacct gtttcgggca
     1741 agggttataa tgcagagtac gggctgctgg gatccaatat ggctacggag accagcatta
     1801 aactgttccc aagggaatgt aacaaaatag ttatggagat tcaggaaacc ctaaagcggg
     1861 aaacaggtaa gcatattgaa gtcatggtct acggggacgg tgcatttaag gatcctcaag
     1921 gtaaaatctg ggagctggct gatcccgtgg tgtcccccgg gtacacgccc ggattggaag
     1981 ggataccaaa tgaaataaag cttaagtata ttgcggacag cgaaagcggt aacattcaac
     2041 ccgaggacat ggctgacctg gtaaagagta ggatcaaaag caaggatcat gatctaatgg
     2101 gcaaagccga atcatcggga actaccccaa gacagctaac cgatttgctc ggcagcctgt
     2161 gtgacctgac cagcggaagc ggcgataagg ggacccctat agtattggtt caggggtact
     2221 tcgataacta tgcgtcagaa taatgattat ccttgtaacc cctatgccga ggtaagaata
     2281 tattcccagg cgtagggtgc tacttacaaa aatggttacg gggtaatagg gggaagttca
     2341 gttaaggttc ttttagggat gaaaccactt tatagtatat taatctaaga aaatgttttg
     2401 cgatatctga aacataatgt ggatgcctat ggcaaaacat aaaattaacg tatcaaggag
     2461 gtcgctataa tgggatttac tattaacgat aaggttactt gggtaggaaa gatagattgg
     2521 gaactgagaa atttccatgg gtatgaatat tccgtggaca agggttccag ctataattcc
     2581 tatcttgtac gggacgaaaa aaccgttctc atagacacgg tgtggaagcc ctttgcaaag
     2641 gaatttgtag aaaatctgaa aaaggaaata gacctgaagg aaatagattt tatagtggta
     2701 aaccatgcag agatagacca tagcggtgcc cttccggaac ttatgcggga gatccccgat
     2761 acccccatat attgtaccgc caacggtatt aaatccataa agggtcattt tcacgaggat
     2821 tggaacttca tacccgttaa aaccggggat accctaaaca tcggccagaa tgatctgatg
     2881 tttgtagagg caaggatgct ccattggccc gacaccatgt ttacatatat gacaaatgat
     2941 aatattttat ttagtaacga tgcctttggt cagcactatg cctcggaata catgtataac
     3001 gaccttgtaa atcccgatga actctacagc gaggcaataa aatactatgc gaatatacta
     3061 aaccccttca gtgtcctagt tcagaggaag attgaagagg tcatcaaact cgatataccc
     3121 ctggatatga tatgtccaag ccatggcatt atctggagaa aggatcccct gcagatagtg
     3181 gaacaatata tgagttgggc ccaggcctac caggaggatc aaattagtat catatacgat
     3241 accatgtggg agggaaccag gcgtatggct gaggcccttg ccgaagggat aaaagagggc
     3301 gatgataggg ttaatataaa gctgtataac acatcaaaga ctgataagaa cgatgttatg
     3361 acggagattt ttaaatcaaa agccatattg ttgggttcac ccacggttaa tggaagcatc
     3421 cttacttcca tggcggccat tgtggacctg gccaagggcc ttaagtttaa agacaagaag
     3481 gcggctgcct ttggaactta cggttggagc ggtgaatccg taaaggtcct cacccagcac
     3541 ttggaaaatg caggttttga ggtcgtcaat cagggtattc gtgaaatgtg gaatcccgac
     3601 gaagaaggcc ttgacaggtg cagagaattt ggcagaaatt ttgtgcagga actgtgagac
     3661 attatagata ggatcgaata gtagaaattg atccgtacaa cagtaaaagc cggcataccc
     3721 tagagtatgc cggctttttt tggactggga gtatgaaatt aaaatttaat caattaccta
     3781 tgagtaccga ggaaaaataa agctatggtt cccggcccgg agtgagaacc gataaccggt
     3841 ccaacgtaat ttataaggaa gtctttgaca ccaagctggt cccttatcat atccgctaaa
     3901 tagttggcat cctcaaggga atcaccgtga cttataaata ccgtttgctc ttccgggttt
     3961 atagcggttt cctgcatttt ttccaaaaga ccttttaggg actttttcct tcccttgacc
     4021 ttggtaacgg gtattaaacg gccctcatcg tccacatgga gtataggctt aatgctcaat
     4081 atgctgccta taaaagcagc ggcccccgat accctgccac ctcttcttaa gtgttccaaa
     4141 tcgtctacag tgaaccaatg gactaacctg agcttattat tttctatcca ttccacgatc
     4201 tgatggtaac ctaacccctg atccctaaga tccagggcca tatgaaccaa taacccaagg
     4261 cccatagagg cacagcgggt atcaattatg gatatggctg catcgggata ctcctccaac
     4321 agcatttgcc ttgctagcac agcgctgtta aagctgccgc tcagagccga agaaaagcac
     4381 atataaataa tcggttgccc ttgttccaca tattgtctaa aaaagtcaat ataggtctgg
     4441 gtatttactt gtgaagtggt cggcatctga cctcccttga ctccgtcata aaaatcccta
     4501 taagacatgg tctgtcccag atcatcttta cgatcgaccc ccatgaaatt gtatgtaaaa
     4561 cttaggatgg gaagattgcg tttttttaca tattccaggg gtagatcact gcaggagtcg
     4621 gttattataa ttggctgcat gataaacctc atttcacaaa atattagtcc ttaattatat
     4681 gaattactta tgaaaacaaa ttgggtagct gagccgcagc acccgggaga tgaaactatt
     4741 ataccatagt tggtgtaatt tcatagcatt aatttacatg cagaccagct cttatgtatt
     4801 ttgtaccttt gtacgattta tataagaaat gcaacgcttc ctaaagggaa gcaattgcat
     4861 tcaatggtat aaagggttag attatgcttg gtctgacaga ttaaattcag agtatataat
     4921 tcctcatcaa atcgtccaag gtatccctgc gcctaatgag aacatctctg ccctcagctg
     4981 ttataaggac ttctgcgggt cttagccgac tgttgtagtt agaggacatg caatagccgt
     5041 aggcaccggc atccataatt cctataatat ccccctccgc aatctccggt aggagcctat
     5101 cttttgcgag gatatccccg ctttcgcaga tgttgccgac gacagtggcc tttctgaaat
     5161 tttcttcggg gactccatat tttttgtata tttcaatgtc gtggtgcgaa tcgtagagca
     5221 tgggccgggc cagcacgtta aatccgatat ccgttccgat gtagtttgtt ctatgatttt
     5281 ccttgagggt gtgtaccgta cctaaaactg ctccggattc tgctacgatg ta
//
LOCUS       DASRNX010000094         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_497453,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000094 DASRNX010000000
VERSION     DASRNX010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497453"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFD89_03665"
     CDS             <1..196
                     /locus_tag="VFD89_03665"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZJ57319.1"
                     /translation="FNKNIVRNQGECMDCGACTAVCPSGALFMDQNDWKLNFDESKCL
                     VCELCVRACPVRAIDVNMFG"
     gene            276..998
                     /locus_tag="VFD89_03670"
     CDS             276..998
                     /locus_tag="VFD89_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HZJ57320.1"
                     /translation="MGEERFYRRLHGSNDLVYFNVKVKETDLDIGANWLLVNESTELV
                     KRCRRDIEDYIKTDPKFLASLDPLSCGVNAPPIIKEMCRAAIKAGVGPMAAVSGAISQ
                     YVGRELSGLTREIIVENGGDIYIKSDRDRIIGVYAGESPLSSKVGIKIRAGDCPMGIC
                     TSSGKVGHSLSFGYADAVVILASDTILADAVATAAGNLVKTADNIQDAIDFAKGIEGV
                     TGTVIIMEDKLGAWGDVELVRL"
     gene            1229..1305
                     /locus_tag="VFD89_03675"
     tRNA            1229..1305
                     /locus_tag="VFD89_03675"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Arg,seq:cct)
     gene            complement(1497..1967)
                     /gene="mscL"
                     /locus_tag="VFD89_03680"
     CDS             complement(1497..1967)
                     /gene="mscL"
                     /locus_tag="VFD89_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HZJ57321.1"
                     /translation="MWKEFKEFATRGNVMDLSIGVIIGGAFGKIVTSLVGDIIMPVIS
                     LLTGKVTGNVDFTNLFISLDGTNYRTLAEAAENGAATLNYGMFISVLIDFFIIAFSIF
                     IMVRQMNKLRPQPAPVEPTTKACPFCISDISIEATRCPNCTSILDAEEIQEGVN"
BASE COUNT          701 a          430 c          578 g          688 t
ORIGIN      
        1 ctttaataag aacatagtaa gaaatcaagg tgaatgcatg gattgtgggg cttgtaccgc
       61 tgtctgccca tcgggggccc tgtttatgga tcagaatgat tggaagctga atttcgatga
      121 gagcaagtgc ctggtatgcg aattgtgtgt cagggcctgt ccggtaagag ctatagatgt
      181 gaatatgttc ggctagttat agataaacaa tacgtaaact aaatttatct gcatgataaa
      241 tgcgatggtt ctatgcgggg aaggcaggat ttaccatggg ggaggaaagg ttttatagac
      301 gacttcacgg ttctaatgat ctggtatatt ttaatgttaa ggtaaaagaa acggatctgg
      361 atattggggc caattggttg ctggtgaatg aatccacgga attggtcaag cggtgtcgcc
      421 gggatataga agactatata aaaacagatc ccaagtttct ggcttctctt gatccgctat
      481 catgcggggt taacgcccca ccgataatca aggagatgtg ccgggctgct ataaaggccg
      541 gagtaggccc tatggcggct gtatccggcg ccatatccca atatgtggga cgggagttat
      601 cagggcttac ccgggaaata atagtcgaaa acggtgggga tatctatata aaaagtgata
      661 gggatagaat aataggggtt tatgctggcg aatccccctt atccagtaag gtcggaataa
      721 aaataagggc aggggattgt cctatgggaa tatgtacctc ttcgggaaag gtgggccatt
      781 ccctgagctt tggatatgcc gatgctgtgg tgattttggc ctcagatacc attttggcag
      841 acgcggtcgc tacggctgca ggcaacctgg ttaagactgc ggataacatt caagacgcaa
      901 tagattttgc taaggggatc gaaggtgtta ccggaacagt tatcataatg gaggacaaat
      961 tgggggcttg gggggatgtg gagctagtca ggttgtaatt gtttatctgc ggacaataaa
     1021 tagcaaactt gcgcaataac ttgagcaggt aatgacttaa gcagttaata ttatccgttg
     1081 tcctgagggt aaatggatag ggcgagatgt aaagtggaga aaaactgata tttgactttt
     1141 taggctggat agtataaaat aatttcgttg cgtatgttat gaaaaaaact ttactggaaa
     1201 ttgggtagac caatacctca aattatatgc acccgtagct caggcggata gagcaccggt
     1261 ttcctaaacc gggtgtcggg ggttcgagtc cctccgggtg tgccagaaaa cccagtcact
     1321 gcaatggttt gcggactggg tttttattac acctacgaat attattccgt tttgttgaca
     1381 ccctgttgtc gctatataaa aactgtacaa aatataggat tcacgtttgt ttttggggct
     1441 tacccgtctt tatatacttt ttcgacgcat attatggata tttaggttaa tgactattaa
     1501 tttacgccct cctggatttc ttccgcatcc agtatcgatg tacaattagg gcatcgggtg
     1561 gcctctatgg agatgtcact gatgcaaaag ggacatgcct tagttgtggg ctcgacaggt
     1621 gccggttggg gccttaattt gttcatctga cggaccatga taaagataga gaatgcgata
     1681 ataaaaaagt caattagtac ggaaataaac atcccgtagt ttaatgtagc ggctccattt
     1741 tctgcggctt cagccaaggt cctgtagttt gttccgtcca ggctgataaa gaggtttgta
     1801 aagtctacat tcccggtaac ctttcccgta agaaggctta ttaccggcat aatgatatcg
     1861 cccacgagag atgtaactat ctttccaaaa gctcccccta ttatgacacc gatggacaag
     1921 tccataacgt ttcccctagt cgcaaattct ttaaattctt tccacataac tttcatcagc
     1981 tcctctgtta tatagttgtc gagccaagca gtggggtatc ccccaagcct accaacggtg
     2041 aattacaacc gtataatata ttctaacatg gccgactata aaatggaaac ataatagatg
     2101 aacaagataa ataaacaaac aatgggtata tttaaattca ataagaaatc cagtgcttca
     2161 aaaaaatgaa ccatgtattt attgaagttt gcttcccttc atagtagaat acatattatt
     2221 gtgatatact gtattatgat aataaggcga agacccgtga cttaaaacgc aggcgaaagc
     2281 ccgtattttc tagatatata ttacgatata tggcattgat gaatttgcac ataaaacgat
     2341 tctttgaagg ggcgaatgcg ttattaaagt ttttttacat attataaata ataatat
//
LOCUS       DASRNX010000095         7550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_443399,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000095 DASRNX010000000
VERSION     DASRNX010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443399"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..794)
                     /locus_tag="VFD89_03685"
     CDS             complement(237..794)
                     /locus_tag="VFD89_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57322.1"
                     /translation="MRITNRERIFISVAILVLLAALYYSLFYRDLSQEIMDIKHSIRW
                     DSSELEKIIQSKGNVQSLYRDIDSLKAKTSKITDNETNSQIEPRIIVFLEDSIKNLGL
                     STKIRFGDPIEYDRCDVIPVTLDLESSYINCRKILGGIEKSPWPYSIDYIKTSKREGT
                     PGHSSNSWDVEMVISFVSFHSDKEP"
     gene            complement(791..1381)
                     /locus_tag="VFD89_03690"
     CDS             complement(791..1381)
                     /locus_tag="VFD89_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57323.1"
                     /translation="MKADINLGIPGESKRIYLILVAASILVLLMVSIFMVPIHRRNLL
                     RAEYIEINRKLLVEGENYRTCIDLENYSANLFKKVEILGKVDTRRGDVGIIIDLIDKY
                     FSKNMELSAITISKNIVNLNGTAPNDGIIADSVLMLQGDERVTRTRIEEISLDTKSLK
                     RVFNISFTTLLNNTDMDGILSGDKDDQELDQGGVMD"
     gene            complement(1378..2415)
                     /gene="pilM"
                     /locus_tag="VFD89_03695"
     CDS             complement(1378..2415)
                     /gene="pilM"
                     /locus_tag="VFD89_03695"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HZJ57324.1"
                     /translation="MELRQKKIIGLDIGTRNIKVIGMNRTGVCGKWSIGSIPQGLVSS
                     HGILAEDLLAGEIRDLIKENEIRGRRCSFCLPSQHVITRSVFHPSMDSDVLIENIRYD
                     MEDYLPLDADEYYIDYRITKTVIINGREHWHILITAAPKKIVLSYLRVLKMAGLNPIY
                     IDVPSNCLEKLLISTTPKTDLSNENNGNLCIVDIGCLSINIIIFSGGRYFIDKTFSLE
                     DGVVSPVNQRGSNGTGHSSDNLPNDINPAIKQTQSKLVHIGDEIIKTIRYYENQMSGD
                     APINEIILLGGGSLIPGLPGSLMKNIGISVITFSDWMDRKKTGGISGLTGTDLMLMGN
                     AIGSTIRRDSL"
     gene            complement(2439..2948)
                     /locus_tag="VFD89_03700"
     CDS             complement(2439..2948)
                     /locus_tag="VFD89_03700"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HZJ57325.1"
                     /translation="MPKFGSRGSAILLVLLVLLILVILGMTLSNMDKVQMKILSTDKV
                     YQASYYYAEAGLTQHIEILRMNMEQLYKDPRTDGRDALLAGLIRVPATTPEFSDYGGE
                     KVKLKVTCIKNKAVIHRDELIIMSICDIGKISCSVKAIVEVRWMDPLDPNFRLDDTSL
                     SIVGWEEIR"
     gene            complement(3103..3540)
                     /locus_tag="VFD89_03705"
     CDS             complement(3103..3540)
                     /locus_tag="VFD89_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZJ57326.1"
                     /translation="MSFITQAISIRQKGLTLMELIIAASLILIVLSLGYSIYLFGIQG
                     YIENAEVIESQSNVRIALEHIAYNIRRTKGVKISDERLIMGSESYKLSGDILMNKDNQ
                     LAVGISEFIFYKPKPSLVYVGISGMPDRNGDVFSLEAYFYLIE"
     gene            complement(3582..4313)
                     /locus_tag="VFD89_03710"
     CDS             complement(3582..4313)
                     /locus_tag="VFD89_03710"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZJ57327.1"
                     /translation="MKNNRGISLIEILVSILILALIVGPFTGIFIQSRMVRRTMSKHL
                     NAMYAVRNEMEILMSMNSGEVYSSNGDKTVGDLLIRISAYPYSAVENSDCIYITSKEI
                     GGLGDEILVLAPGGCKTFIVDAGEDLYKMQIAIDGSSYQINSGKDLISGCLSFVNRPS
                     IQINLTDKSPDILYDFNVYGDADIAVYPGNHDSWCLASNGAYRVVDRCFYRDYCVYRV
                     KVGAFVDPGSNNPIFEIEGIITVKD"
     gene            complement(4310..4765)
                     /locus_tag="VFD89_03715"
     CDS             complement(4310..4765)
                     /locus_tag="VFD89_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZJ57328.1"
                     /translation="MKSSRGFTLIELICVIAILGILAFIAIPAITDTLDKWTLDSTAQ
                     QIMEDIRLAQHLTITNAISHYFELDTKNRFYRIKSIAMRDPTIKTIEFSPSIASISST
                     LKNEGNLKRLTFAATGIPGQTGSIVLRTKKGKEKTITIAVGTGRVAISP"
     gene            complement(4889..5656)
                     /locus_tag="VFD89_03720"
     CDS             complement(4889..5656)
                     /locus_tag="VFD89_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HZJ57329.1"
                     /translation="MIYLAIAILSVFLGSFYNVCIHRILREESVIYPPSHCPDCGHSL
                     GPLDLVPVFSYVFLMGRCRYCKARISPRYPLVEVLTSITYLLLYKVFGLSWSLVAYSF
                     FLGVLIIITFIDIEYQIIPNSLILLGLIFGILFGAINITTGIVDALLGLVIGFGSLLV
                     IAILAKVFIGKEGMGGGDIKLLGVIGLFLGWKLTLLTLILSIYIGGVFSILLLLLKIK
                     KRGEYIPFGPFISLAGLIALLWGNSIIQWYVGISFPI"
     gene            complement(5671..6873)
                     /locus_tag="VFD89_03725"
     CDS             complement(5671..6873)
                     /locus_tag="VFD89_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZJ57330.1"
                     /translation="MARYRYIAVNQDGQEVEGVLEADGKRDAVSILRQREFIPLLIKT
                     AKGGGGISDIQIYPRASLKDLAIFCKQYASLLQAGVPPVQGLYTLVNQTNNYSLRQAL
                     QRVYHLVQDGHSLSQGMLKQEGNFPTILIRTVESGEWSGDLETSFERMADYFERQYKI
                     KQKVRRALTYPALLIVTSIIIIAFLMHIVVPQFVTLFKSSQAQLPKSTRILMGFYNIS
                     VHNTWIFLVFPASFYLMVKALLSHKGSRVFLDRILLKMPLLGKLNIKIISARFHRTMA
                     ILISAGLPITQSLAISSKVADNAFVMSKLQQAIGMVYQGRGLAKSLVALDVFPLETVN
                     IIAIGEESGKIGEMMDKIADLCELEAETALDRFVSLLEPLSILFLGGIIAYIVISVVL
                     PMFQMYVF"
     gene            complement(6914..7453)
                     /locus_tag="VFD89_03730"
     CDS             complement(6914..7453)
                     /locus_tag="VFD89_03730"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZJ57331.1"
                     /translation="MGSHKSFTIKYKAVATSKRGVTLIELIMVLALMAIVFTSIFSFY
                     SFNLGIFGRAQNKSDIQHDVLLVSEFITKEVRTATGISLSSRPPQDNDKESYNSFIGL
                     YQDGVEYHYDKGYRQIANTNIMDLNLVLSPNSNLLKFHITGEKDGEEYRIDSNVSLPN
                     LADMTDEEISGSWIYYNKP"
BASE COUNT         2146 a         1752 c         1374 g         2278 t
ORIGIN      
        1 cccccaggta atgctcgaca accttatttc gaagagcatc ctatatgtac tctattcgcc
       61 ttgattgcgt ttattatatc ataagtgcaa gcctcgattg gcttgcttca caccgattca
      121 ataaacgcat tgggtaaaac ccataggttt tacttctttg attgcgttta ttataacata
      181 tccgagaccg gggcttcaaa cccaaatccg ctgcatgctt gcttataaag agcatatcaa
      241 ggctctttat cactatggaa ggacacaaag cttataacca tctccacgtc ccagctattg
      301 gatgaatgcc ccggggtacc ctccctttta ctggttttta tataatcaat ggaatagggc
      361 cagggcgatt tttctatgcc gcccaggatt tttcggcaat ttatataact gctctctaaa
      421 tcaagagtga cgggtattac atcgcatcta tcgtattcta tagggtcccc gaaccttatc
      481 tttgtggaca gacccaaatt cttgatggaa tcctctaaaa agactatgat tctaggttca
      541 atctgggaat tcgtttcgtt gtccgttatc ttactcgttt tggctttgag gctatcaata
      601 tctctataca gactctgaac attccccttt gattgtatga ttttctccag ctcactgctg
      661 tcccaccgga tactgtgttt aatatccata atctcttggg ataaatccct ataaaacagg
      721 ctatagtaca atgccgctaa aagaaccaga attgctacag atatgaagat cctttccctg
      781 tttgtaattc tcaatccata acacccccct gatcgagttc ctggtcatcc ttgtccccag
      841 ataggatccc atccatatca gtgttattaa gaagggttgt aaagcttata ttgaaaaccc
      901 tctttaggct ttttgtatca aggctaatct cctctattct ggtccttgtt accctttcat
      961 cgccctgcag cattaaaacg ctgtcagcta ttataccgtc gttaggtgcg gttccgttta
     1021 agttcactat attctttgat atagttatag ctgacaattc catgttcttg gaaaaatatt
     1081 tgtctatcaa atcaataata attccaacat ccccacgtct tgtatctact ttcccaagga
     1141 tttctacctt tttaaacaaa ttcgcggaat aattctccag atcaatgcaa gtcctatagt
     1201 tctccccctc taccagcagc ttccgattaa tttcaatgta ttcagccctt aataagttcc
     1261 ttcggtggat gggaaccata aagatggata ccataagcaa gactaaaata gatgctgcaa
     1321 ccaaaatcag atagatgcgt tttgattccc cagggattcc caggtttata tctgctttca
     1381 aagtgaatcc ctcctaatgg tagagccaat ggcatttccc ataagcataa gatccgtccc
     1441 ggttagaccg cttatacccc cggtcttttt cctatccatc caatcactaa aggtaattac
     1501 agatatccct atgtttttca ttaaagagcc cggtagtccg gggatcaagg aacctccgcc
     1561 caataggatt atttcattta tcggtgcatc gcccgacatt tgattttcgt aatatctaat
     1621 ggtttttatt atctcatcac caatatggac cagcttgctc tgtgtttgtt ttatggccgg
     1681 attgatatca ttagggagat tatccgaact gtgacccgta ccattgctac ccctttgatt
     1741 aacaggggat acaactccat cttctaaaga gaaggtcttg tctataaaat agcgaccccc
     1801 gctgaatatg attatgttaa tggaaaggca tcctatatcc actatgcaga ggtttccgtt
     1861 attttcattt gacagatccg tcttgggagt agtgcttatg agtaattttt ctaaacaatt
     1921 ggacgggaca tctatgtata ttggatttaa ccccgccatc ttcaataccc taagataaga
     1981 cagaacaatt ttcttcggag cagctgttat aagaatatgc caatgctctc tcccgtttat
     2041 tatgacggtc tttgttatcc tataatcaat gtaatactcg tccgcatcaa gaggaagata
     2101 atcttccata tcgtagcgga tgttttctat taagacatcg ctatccatag atggatgaaa
     2161 aacagacctg gttattacat gctgggaggg caaacaaaat gagcatctcc tacctcttat
     2221 ctcattttcc tttatcaagt cccttatttc tccggccaat aaatcctctg caagaatccc
     2281 atggcttgaa actaacccct gcggtatgga gccgatagac catttcccgc agactcccgt
     2341 tctgttcatc ccgataacct tgatattgcg ggtccctata tccaatccaa tgattttctt
     2401 ctgcctcagt tccatgatat ccccccaaat ttctaaggtt atcttatctc ctcccacccc
     2461 actatcgaga ggcttgtatc atccagccga aagtttgggt ccaaaggatc catccacctt
     2521 acctcaacta tagccttaac tgaacaactg atcttcccga tatcacatat cgacatgata
     2581 atcaattcat ccctatgtat aaccgcctta ttctttatac atgtaacttt tagttttact
     2641 ttttctcctc cataatcgct aaattccgga gtcgttgccg ggacccttat gagccctgcc
     2701 agcagcgcat ccctaccatc ggtccttgga tctttatata attgttccat attcatccgc
     2761 aatatttcta tatgctgggt cagaccggcc tcggcataat aatatgaggc ctgatatacc
     2821 ttatccgtag ataaaatttt catttgcacc ttatccatgt tggataaagt catccccaag
     2881 attacgagaa ttaacagaac cagcaatacc aacaatatgg cggaaccccg agagccgaat
     2941 tttggcatta tatccccctg acaaaacact tatgcgctct tttgtgatta tcatctatag
     3001 aacaaatttc gctaaaatct gccgctatat ctgccgttaa cagaaccata ctaatcccga
     3061 atatagcggc taatgtattt ttgatactgc atgggattgt attcactcta tgagataaaa
     3121 atatgcttct aacgaaaaga catcaccgtt tctatcgggc ataccggaaa ttcccacgta
     3181 gactagggag ggttttggtt tataaaagat aaattccgat attcccaccg ctaattgatt
     3241 gtccttattc attaggatat ctccggataa tttgtagctt tcactcccca ttatcagtct
     3301 ctcgtcgctt attttaactc ccttggtcct tctgatatta taggcgatat gttccagggc
     3361 gatacgaacg ttggattgac tttcaatcac ttcggcattt tctatatatc cttgaattcc
     3421 aaacagatat atggaatacc ctaaggatag tacgatcaaa attagggagg cagcaatgat
     3481 cagctccatt agggtcaatc ctttttgcct aatagatatt gcctgggtta taaaggacat
     3541 cttcgtatca cccctatctt tatgccggat ctttaacaac gctaatcctt aactgtaatt
     3601 ataccctcta tctcaaagat aggattatta gaaccgggat cgacaaatgc cccaaccttg
     3661 actctataca cgcaatagtc cctgtaaaaa catctgtcca caaccctata ggccccattg
     3721 gaagccagac accagctatc gtgatttccg ggataaacgg ctatgtctgc gtctccgtaa
     3781 acattgaaat cataaagtat gtcaggtgat ttatctgtta ggtttatttg gattgacggt
     3841 ctgttgacga aactaaggca cccggagatt aaatccttcc cgctattaat ttgataggac
     3901 gatccgtcta tggctatctg catcttatat aggtcttcac cagcatcaac aataaaggtt
     3961 ttgcatcccc ctggcgccag aactaagatc tcatccccca agccaccaat ctccttagat
     4021 gttatataga tacagtctga attttccact gcgctgtagg gataggccga aatgcggata
     4081 agcaagtccc caacggtttt atcaccattt gagctatata cttccccaga attcatggac
     4141 atcaatattt ccatttcgtt gcgtaccgca tacatagcgt tcaaatgttt ggacatcgta
     4201 cgccgcacca tccttgattg aataaagatg ccggtaaatg ggccaacgat caaggctaag
     4261 ataagaatag agacaagaat ttctatcagg gaaatgcccc tgttattttt catggactaa
     4321 ttgccaccct ccccgtcccc acggctattg tgattgtctt ttctttgccc tttttggtac
     4381 gaagtacgat gctgcctgtc tgacccggaa tgcctgtagc ggcaaaggtc aatctcttga
     4441 gattgccctc gttctttaag gtgctgctaa tactggcaat gctcggacta aattctatgg
     4501 tctttatggt agggtcgcgc atcgctatgc tctttatcct gtagaatcta tttttggtat
     4561 ccagttcaaa gtaatggctt atggcgttgg ttatggttaa gtgctgtgcc aacctgatat
     4621 cctccattat ttgctgggca gttgaatcca gggtccattt atccagggtg tcggtgatgg
     4681 cgggaatcgc tataaacgcc aaaatgccca gtatggcaat aacacaaatc aattcgatta
     4741 aagtaaatcc ccgcgagctt ttcataccaa atcaccgcct catgggtact ggttttagct
     4801 tatgtccgaa aatgagtgta tagaggattt caaatgcatt tgtagtcaac aaggtgtcat
     4861 aaggtctcgt tcaggccttt aaagatgctt atatagggaa tgaaattccc acataccatt
     4921 gaatgatgct attgccccat agaagggcta tgagacctgc aagggaaata aatgggccaa
     4981 agggtatata ctcgcccctc ttcttaatct ttaacaggag aagaagtata gagaataccc
     5041 cacctatata tatcgatagt attagggtga gaagtgtaag tttccatccc aaaaataggc
     5101 cgataacccc caataatttt atatccccgc cgcccatgcc ttcttttcct atgaatactt
     5161 tagccaaaat agcgatcacc aggaggctac caaagccaat aaccagccct aacaaggcat
     5221 ccactatccc ggtcgtgata tttatcgcac caaacaatat tccaaaaatt agccccaaaa
     5281 gtattaggct gttgggaatg atttgatatt ctatatcgat gaaggtgata attatcaaaa
     5341 cccctagaaa gaatgaatag gcaacgagac tccaacttaa gccaaagact ttatatagaa
     5401 gaagatacgt gatggaagta agaacctcca caagcggata ccttggagaa attcttgcct
     5461 tgcaatacct acatcttccc attaggaaaa catagctaaa gacgggcacc aaatccaaag
     5521 gacctaagga gtgtccacag tcagggcaat gagatggcgg atatataacg gattcctccc
     5581 ttagaatcct atgaatacat acgttataaa agcttcctaa aaatacagat aatatagcta
     5641 tagctaaata aatcataaat aaacccccaa ctagaataca tacatctgga acatgggcag
     5701 cactaccgaa ataactatat acgcaataat cccgcctaga aataggatgg aaagtggttc
     5761 cagtaaggat acaaacctat caagagccgt ttctgcctcc aattcacaga ggtcagctat
     5821 cttatccatc atttccccta tctttccact ctcttctcca atagcgataa tatttaccgt
     5881 ctccaatggg aaaacatcta aggctactag ggatttggct aaccctctac cctgatagac
     5941 catacctatt gcctgttgca acttactcat tacaaatgcg ttatcggcta ccttagagga
     6001 aatagccaat gattgtgtga ttggtagacc cgccgaaata agaatcgcca tagtcctatg
     6061 aaagcgggca gatattattt tgatattgag cttccctaaa agaggcatct ttaatagaat
     6121 cctatctaaa aagacccgac tccccttatg ggataataag gctttgacca taagatagaa
     6181 cgatgccggg aaaaccaaga atatccatgt gttatgtacg gagatattat aaaatcccat
     6241 taagatccgc gtggatttgg gcagctgggc ctgggaactt ttaaataggg tgacaaattg
     6301 gggaaccaca atatgcatta aaaaggcgat aattataatg gatgtaacga tcaacagggc
     6361 cggataggtt aatgcccttc gaaccttttg ctttatcttg tattgccttt cgaaatagtc
     6421 cgccatcctt tcaaaggagg tctctaggtc cccgctccat tcccctgact caacggttct
     6481 tataaggatc gtagggaaat tgccttcctg ttttaacata ccttgggata aggaatgtcc
     6541 atcctgcacc aaatgataaa ccctttgcag tgcttgcctt agagaataat tgttcgtctg
     6601 attcaccagg gtataaagcc cctgtaccgg gggcactccc gcctgtaata gggatgcata
     6661 ttgtttgcaa aatatagcta gatcctttaa cgaagcccta ggatatatct gtatatcaga
     6721 aatacccccg ccccctttgg cggtcttaat tagaaggggg ataaattccc tttgacgtaa
     6781 tatagatacg gcatcccttt tcccatcggc ttctaaaacg ccttctacct cttgtccgtc
     6841 ttggttaacc gctatatatc tatatcttgc cattttgtat cactccataa atatcgctat
     6901 tgcccttata ggcctacggt ttgttatagt agatccaaga acctgaaatc tcctcatccg
     6961 tcatgtccgc cagattagga agggatacat tagaatctat cctatactct tccccgtcct
     7021 tttcaccggt aatatggaac tttaataaat tactattggg ggacaaaacc aaatttaggt
     7081 ccataatatt tgtatttgct atctgtcggt atcctttatc atagtgatac tctacaccgt
     7141 cttggtaaag ccctataaag ctattgtagg attctttatc attgtcttgg ggtgggcggg
     7201 aggacaagct gatgccggtt gctgtcctta cttcctttgt aataaattcc gataccaaaa
     7261 gtacatcatg ttgaatgtcc gatttattct gcgccctacc gaagataccc aggttaaagg
     7321 aataaaagga aaagattgag gtgaaaacta tggccataag ggcaaggacc ataataagct
     7381 ctattagagt gacacccctc tttgatgtgg ccaccgcctt gtattttatt gtgaaggact
     7441 tatgagaacc catgcgcctc caaccttcct acatttatga gaaatttggc tttacttatc
     7501 tatttgggaa taaatactga aatagctgat ttttttcctt tgacctctat
//
LOCUS       DASRNX010000096         4189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_603316,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000096 DASRNX010000000
VERSION     DASRNX010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603316"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VFD89_03735"
     CDS             <1..836
                     /locus_tag="VFD89_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HZJ57332.1"
                     /translation="EYYRDMGYSVAIMADSTSRWAEALREMSGRLEEMPGEEGYPAYL
                     GSRLAEFYERAGRVICYGGDEREGAISAIGAVSPPGGDISEPVTQATLRIVKVFWRLD
                     AMLAYRRHFPAINWIESYSLYLDRLSQWMRDEVSEDWDRLRAEAVRLLHQESELEEIV
                     RLVGIDALSLEDRLTLETSRSIREDYLHQNAFHDVDTFTSLNKQHKLLRLILEFYSQG
                     RRALQEGAEFSEIVNLPVRERIGRAKYTPEEELDTLDGIMRDLREEMSALTAEGGFEY
                     A"
     gene            829..2217
                     /locus_tag="VFD89_03740"
     CDS             829..2217
                     /locus_tag="VFD89_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HZJ57333.1"
                     /translation="MLKEYRTITEVVGPLILVEDVEGATYNELVEIEQQTGEIRMGRV
                     LEVDGDKAVVQLFEGSQGLQISKSKARFLGKGIELAVSPDMLGRVFDGMGRPQDGGPR
                     IIADKRLDIEGEPINPSARDYPSEFIQTGISAIDGLNTLVRGQKLPVFSGSGLPHAQL
                     AAQIARQARVLGGESNFAVVFAAMGITFEEADFFISDFRRTGAIDRTVLFMNLANDPA
                     IERIATPRMALTAAEYLAFDLEMHVLVIYTDITNYADALREISAARKEVPGRRGYPGY
                     MYTDLATMYERAGRVRGGEGSITQIPILTMPEDDLTHPIPDLTGYITEGQIILSRDLH
                     GKSIVPPIDVLPSLSRLKDKGIGEDKTREDHADTMNQLFAAYARGKEAKELAAVLGDA
                     ALSDVDKLYAEFADEFERRYVSQGYDTDRSIEETLDLGWELLAILPRTELRRIRDEYL
                     DKYLPRTQEGNE"
     gene            2221..2880
                     /locus_tag="VFD89_03745"
     CDS             2221..2880
                     /locus_tag="VFD89_03745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HZJ57334.1"
                     /translation="MAVLRVNPTRMELSRLKKRLAVAVRGHKLLKDKRDEMMRRFIEL
                     IKRNMKLREQVEAELTSALGDFLMARAVMSPEVLEEAVMFPTREVELKVSKRNIMSVD
                     VPKIDYEEKSHEGLSPYPYGFVSTSAELDGAIVTLSDVLSRMLELAEIEKTCQMLADD
                     IEKTRRRVNALEFIMIPQMEETIRYITMKMDEDERSSRTRLMKVKDMIEKRKAESADS
                     H"
     gene            3121..3768
                     /locus_tag="VFD89_03750"
     CDS             3121..3768
                     /locus_tag="VFD89_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZJ57335.1"
                     /translation="MGWEMVLRLVLALIIGGTIGAERERSNRPAGFRTHTLVCVGSAL
                     VFLTSLFIFERYHHLVNLDPTRLPAQVVSGIGFLGAGTIIHYGSNVRGLTSAATLWVV
                     ACLGLAIGAGFYWGALATWIAVYIILIFLKRIELSFLRNKGSTRITLELRNTPEIIGK
                     VADVMGAFNIKIREIRLLPSDDQWAVANFLVDMPRYMDKEVLMGRLSEIEGVQLD"
BASE COUNT         1094 a          939 c         1204 g          952 t
ORIGIN      
        1 cggaatacta cagggatatg ggctacagcg tagccatcat ggcggactcc acctcaaggt
       61 gggccgaggc cctacgggag atgtccggac gcttggagga gatgcccggc gaggaaggat
      121 atcctgctta cctaggctcc agactggccg aattctatga gcgggccgga cgggttatct
      181 gctacggtgg ggacgaaagg gaaggggcca taagcgccat cggtgcggtt tcaccccccg
      241 gcggcgatat ttcggagccc gttacccagg ccaccctccg tatagtcaag gtattttgga
      301 gacttgacgc catgctggcc tacagaaggc actttcccgc catcaactgg atagagagct
      361 attccctata cctagacagg ctgagccaat ggatgaggga cgaggtgtcc gaggattggg
      421 atcgcttaag ggccgaggcg gtaaggctgt tgcatcagga atctgagcta gaggagatag
      481 tgaggctggt ggggatagat gccctttcct tggaagatag gctgaccctg gagacctcca
      541 ggtccattag ggaggactac ctgcatcaaa atgcctttca cgacgttgat acctttacct
      601 ccctcaacaa gcaacacaag cttttaaggc tcatcttgga attttatagt caggggcgaa
      661 gggcccttca agagggagca gaatttagcg agatcgtaaa tttacccgtc agggagagga
      721 tcggccgggc taaatatacc cccgaggagg aacttgacac cttagacggg atcatgagag
      781 atctcaggga agagatgtca gccctgacag cggagggagg atttgaatat gcttaaggaa
      841 tataggacca ttacagaggt cgtaggcccc ctcattctgg tagaggacgt tgagggtgcc
      901 acctataacg aacttgtgga gatagagcag cagaccgggg agataaggat gggccgggtt
      961 ttggaagtag acggagacaa ggccgtggtt cagctctttg aaggatccca aggcctccag
     1021 atatccaaga gtaaggccag atttttgggt aagggcatag agctggcggt atctccggac
     1081 atgcttggca gggtcttcga cggaatgggt aggccccagg acgggggccc aaggataatc
     1141 gcggacaaga ggctggatat agagggagag cctattaacc cctcggccag ggattaccca
     1201 tccgagttta tccagacggg gatttctgcc atagacggtc ttaacaccct ggttcgggga
     1261 cagaagctgc cggtattttc cggttccggt ttgccccatg cccagcttgc tgcgcagatt
     1321 gcccgtcagg ccagagtatt gggcggggag agcaattttg ccgttgtatt tgcggccatg
     1381 ggtatcacct tcgaggaggc ggacttcttt atctcggatt ttagaaggac cggggccata
     1441 gacaggaccg tgttgttcat gaatctggcc aacgacccgg ccatcgagcg gatagcaacc
     1501 ccccgaatgg cccttacggc ggcggaatac ttggcctttg atctggaaat gcacgtcctg
     1561 gtcatataca cggatataac caactacgca gacgccttga gggagatctc tgcggccagg
     1621 aaggaagtcc ccggacggcg cgggtatccc ggctatatgt atacggacct ggccaccatg
     1681 tacgaaagag ccggcagggt aaggggcgga gagggctcta taacccagat tccaattctc
     1741 accatgcctg aggacgattt gacccaccca atcccggatt tgacgggata tattaccgaa
     1801 gggcagatta tcctaagcag agaccttcac ggtaagagca tagtacctcc catcgatgta
     1861 ctcccttccc tctccaggct gaaggacaag ggaatcgggg aggacaagac cagggaggac
     1921 catgcagaca ccatgaacca gctctttgcg gcctatgcaa ggggaaaaga ggccaaggaa
     1981 ctggcggcgg tactgggcga tgcggctctc agcgatgtgg ataagctcta tgcagagttt
     2041 gccgatgaat tcgaaaggcg ctatgtatcc cagggttatg ataccgaccg ctccattgag
     2101 gagaccttgg atttgggatg ggagctctta gctatcctgc ccaggacgga gctgagacgg
     2161 atcagggatg aatatctgga taaatacctg ccccggaccc aggaagggaa tgaataggct
     2221 atggctgttt taagagttaa ccccacccgt atggaattga gccgtctcaa aaagcgtttg
     2281 gccgtagccg tgcggggtca caagctccta aaggataagc gtgacgagat gatgagacgc
     2341 ttcattgagc ttattaaaag gaatatgaaa cttagagagc aggtggaggc ggagcttaca
     2401 agcgctcttg gagacttttt gatggccagg gcggttatgt cccccgaggt gttggaggaa
     2461 gctgtaatgt tccccacccg tgaagtggaa ctgaaggtct caaagcgaaa tattatgagt
     2521 gttgatgtgc ccaagataga ttatgaagaa aaaagtcatg agggcctatc cccatatccc
     2581 tacgggtttg tatccacctc cgccgaattg gacggggcta tagtgaccct atcggacgtg
     2641 ctgtctcgca tgttggagct ggccgaaatt gagaagacct gtcagatgct ggcggacgat
     2701 atagaaaaga ccaggcgacg ggtcaatgca ttggagttta taatgatacc tcagatggag
     2761 gagaccatcc ggtatataac catgaagatg gacgaggatg agagaagcag ccgcaccaga
     2821 cttatgaagg ttaaggacat gatagaaaaa cggaaagcag agagtgcgga ttcacattga
     2881 gtccgcattt ttttattggt tccaatccag ccttcccctt aaacaactag cataatgggt
     2941 taatttagta attgcttgta ctatgattat ccattttagt cagggaaagc ggacttttag
     3001 gggatcgggg ggaaaacccc ttcccctttg gggcataggt gttcattcac attgatactt
     3061 tgggataaaa ttaaaaaaat ggtcccataa aagggacaat ccgattggag gtcgatgggt
     3121 atggggtggg agatggtttt gcggttggtg ctggccttga taatcggggg taccataggc
     3181 gccgaaaggg agaggagcaa tcggccggcg gggtttagaa cgcatacact ggtatgtgtg
     3241 ggctcggccc ttgtcttttt gacatccctc tttatattcg aaaggtatca tcacctggta
     3301 aatttagacc ccacaaggct gcccgcccaa gttgtcagcg ggataggttt tttgggtgcg
     3361 ggaaccataa ttcactatgg ctccaatgta aggggcctta cctctgcagc taccctctgg
     3421 gttgtggcct gtctcgggct ggcaatagga gcggggttct attggggagc cctggcaact
     3481 tggattgcgg tttatattat cttgatattc ctcaagagga tcgaattatc gtttctgcgc
     3541 aataagggat ctaccaggat taccctagaa ctgcgcaata ccccggagat aatagggaaa
     3601 gtggccgatg tcatgggtgc atttaatatt aaaataaggg agatcaggct attgcccagc
     3661 gatgaccagt gggcagtcgc aaattttttg gtggatatgc cccgatatat ggacaaggaa
     3721 gtgctgatgg gccgattaag cgagatcgag ggcgtacagt tggattaact gcacccgcaa
     3781 agtccgagca ctctagtaag caggctatgt caggaataat cgcccggact agctgtgccc
     3841 gcgaagctcg gacaccctaa taaagctata tccaaaatac ccaatcagga tggctgtgcc
     3901 tgtgagggcg aaacacattt atgaggccga aacacattta tgaggctatg cccaagccgg
     3961 ctgtgcttac agggcaggag gacataattt ccgactgcta aaaagtatat aatgtttttt
     4021 ttcggcaata atccaaatat agacaaacaa cttcaactat aaaatttatc accatgaaat
     4081 tgcataagcc cgttgcaact atcatgctga taaatttagt tttcgtaatg tttatctaca
     4141 gacaagcaac ttcaactata aaatttatca ccatgaaatt gcataagcc
//
LOCUS       DASRNX010000097         4651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_233877,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000097 DASRNX010000000
VERSION     DASRNX010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233877"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFD89_03755"
     CDS             complement(<1..570)
                     /locus_tag="VFD89_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57336.1"
                     /translation="MSRIVKSDRIVINADKKFSSNSVGLNSVGGRARSHADDESVDAN
                     YSGLIKAARRQAEGIIKSARDNAERITREAQISATEVMETARKEGHTDGYADGYDKGY
                     AKGVSEGQDHGMAQYRDIIQKAMELKQNYLDDYENLYRASERHMLGLSIEIARKVIGG
                     VLENDDRACVELAYNALKQMRGNGKASLRV"
     gene            complement(563..1576)
                     /gene="fliG"
                     /locus_tag="VFD89_03760"
     CDS             complement(563..1576)
                     /gene="fliG"
                     /locus_tag="VFD89_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HZJ57337.1"
                     /translation="MARNKGTINGREKAAILLISLGTERASEIYKHLKEEEIEQLTLE
                     IANLRKVDSEVKDTILEEFYEICLARNYIAEGGIGYAKEVLERALGGQKALELINKLT
                     SSLQVRPFDFARKADPTQLLNFIQNEHPQTIALIMAYLRPQQAAIILSALPLEKQAEV
                     ASRIAIMDSTSPDIIKEIERILEKKLSSMVTSDFTTAGGIESIVDILLSVDRGTEKHI
                     MESLEMQDSELAEEIKRRMFVFEDIVTLDNRSVQRFLREVENNDLALALKTASEEVSR
                     IIFSNISKRLQDMLKEDMEFMGPVRLRDVEDAQQKIVNIIRRLEDAGEIVVSRGGGDE
                     IIV"
     gene            complement(1603..2721)
                     /gene="fliF"
                     /locus_tag="VFD89_03765"
     CDS             complement(1603..2721)
                     /gene="fliF"
                     /locus_tag="VFD89_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HZJ57338.1"
                     /translation="MGQSIRFLEGVEDAVVTIAMPKSDSFVLKEEEIPVSASAIILPK
                     HGYEITGQQAKSIEQLMSTSVPGLDKENISIIDNKMNILNPEGTQDAQTVTNQFVMQQ
                     QLENKIKAEIVGLLEPVFGYGRIKTAVNAKLNFDKRTKESITFEPVMDDSGIIISQEL
                     LKEKMSNNPAGGIPGETNNTTQYPEAGIGGDSTSQKDHKKINYEVNEIKEMIEEEQGK
                     LDNLSIAVVIDNDQLDQQTINGVRELVATAMGTDLERVAVQSWEFNTDIQQELLDALQ
                     GQSREKPWLNASRAVIIVSSLVIVLALIAILHKSRQSIPVEVMEEYIPEESVAVEEDS
                     LPDIAATEEDKIRAQLNRFAEEQPEEIAQLLRNWLSEE"
     gene            complement(2676..3131)
                     /locus_tag="VFD89_03770"
     CDS             complement(2676..3131)
                     /locus_tag="VFD89_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57339.1"
                     /translation="MLRVLESIKQQLNNYWDNLDKPKKRNLIFIIGLIVLVVVFISVL
                     LAKKDYVVLYTGLTEKEAAEVYTKLKEVNAQPKMDGTATILVPKEKEAELRMQMTLEG
                     YPKSGFNYDLYLQGNSFGQTNDEIQKDGLYSFRNVWVSPYVSLRGLRMQ"
     gene            complement(3342..3635)
                     /gene="fliE"
                     /locus_tag="VFD89_03775"
     CDS             complement(3342..3635)
                     /gene="fliE"
                     /locus_tag="VFD89_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HZJ57340.1"
                     /translation="MKIQNLYLNPSGLTESAGTKSATNTPKFKDILENVAQDAQELRK
                     RDIVNNTHMATGQVDNLDRIMIDMEKADIALQFTLQIRNKILDAYQEIMRMQV"
     gene            complement(3650..4087)
                     /gene="flgC"
                     /locus_tag="VFD89_03780"
     CDS             complement(3650..4087)
                     /gene="flgC"
                     /locus_tag="VFD89_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HZJ57341.1"
                     /translation="MIKSMQISASGMAVQRLRMDIIAQNIANMNTTRTARGGPYRRRL
                     ISVQEGIEAKPFSRFLSESSNTFTGHGVEATSVTEDPTPFKTIFDPGHPDADDEGYVH
                     MPNVDNLKEMVDMISATRSFEANVTAFNATKSMAIKALEIGRR"
     gene            complement(4091..4504)
                     /gene="flgB"
                     /locus_tag="VFD89_03785"
     CDS             complement(4091..4504)
                     /gene="flgB"
                     /locus_tag="VFD89_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HZJ57342.1"
                     /translation="MVNGSLFSHTAALERSLDVVWKRHEVISHNISNADTPGYKKKEV
                     VFENELVSALTRRSIQGRQTRKGHLRIGNVPINEIGPKTVDVNSTKMRMDDNNVDIDS
                     EMANLASNTVMHHALIQKLNGEFSRLKSIINEGRR"
BASE COUNT         1126 a         1150 c          817 g         1558 t
ORIGIN      
        1 taccctaagt gaggcctttc cattaccccg catctgcttc aaagcattat aggctaattc
       61 cacacaagcc ctatcatcgt tttctagcac cccgccgata acttttcgcg ctatttcaat
      121 agatagccca agcatatgtc tttcactggc tctgtagaga ttttcataat cgtcgagata
      181 attctgtttt aattccatag ctttttgtat tatatctcgg tattgagcca tgccgtggtc
      241 ttggccttcg cttacaccct ttgcgtagcc tttatcgtat ccatcggcgt aaccgtcggt
      301 atgtccttct tttctcgctg tttccatgac ctcagtggct gatatttggg cctctcttgt
      361 aattctttcg gcattatctc gggcgctttt gattataccc tcagcctgac gtctggcagc
      421 ttttattaat cctgaataat tagcatcaac gctttcatca tcggcatggg atcgggccct
      481 ccccccaaca ctattcaagc cgacactatt ggacgagaac ttcttgtcgg cattaataac
      541 tattctgtcg cttttgacta tcctagacaa ttatttcgtc ccccccaccc cgtgaaacga
      601 caatttcacc ggcatcctca agtcttctga tgatattaac aatcttttgt tgggcatctt
      661 ctacgtcacg gagcctaaca ggccccataa attccatgtc ctccttcaac atatcttgca
      721 gccgcttgga tatgttcgag aaaataatcc ttgaaacttc ctcgcttgct gtctttaatg
      781 ccaaagcaag atcgttgttc tctacctcgc gtaggaatcg ctggacagag cggttatcca
      841 acgttacaat atcctcgaat acgaacatcc ttctttttat ttcttctgcc aattcgctgt
      901 cttgcatctc caaggattcc attatgtgtt tttctgttcc cctgtcgact gataggagaa
      961 tatccacaat cgactctatt ccccccgcag ttgtaaagtc agaagtcacc attgaggata
     1021 gctttttttc taaaattctc tctatttcct ttataatgtc cggagaagta ctgtccatta
     1081 tagcaattct ggatgccacc tccgcctgtt tttccagtgg gagtgcggac aatattattg
     1141 ccgcttgctg aggccttaaa taggccataa tcaacgcgat ggtctgggga tgctcgttct
     1201 ggataaaatt taataattgt gtaggatccg cctttctggc aaaatcaaag ggtctgacct
     1261 gcagggagga agtcaattta ttgatcagtt ccaatgcctt ttggcccccc aaggccctct
     1321 caagaacctc cttggcatat cctataccac cctcagctat atagtttctt gcaagacata
     1381 tctcgtaaaa ttcttcaagg atggtgtctt ttacttcgct gtcaaccttt cgaagattcg
     1441 caatttccag cgttaactgt tcaatttcct cttctttaag atgtttgtat atctccgagg
     1501 ccctctccgt acccagggat atcagtaaaa tagcagcttt ctccctaccg tttattgtcc
     1561 ccttattcct tgccattttc tatccccaac cccttatacg cactattcct cgcttagcca
     1621 atttcttaat aattgggcaa tctcttccgg ctgttcctct gcaaacctat ttagttgcgc
     1681 tctaatcttg tcctcttctg tagctgctat atcgggtaaa ctatcctctt caacggctac
     1741 tgattcttcc ggaatatatt cttccatgac ttccacagga attgattgcc tgctcttatg
     1801 cagtattgca attaaggcca aaactataac caaagaagat acaattatta cagctctact
     1861 ggcgtttaac caaggtttct ccctcgattg cccctgtaag gcgtctaata gctcctgttg
     1921 aatgtctgta ttgaattccc aactctgaac cgccaccctc tccaaatctg tgcccatagc
     1981 cgtagctacc aattccctga ccccgttgat agtctgctga tccaattgat cgttatctat
     2041 cacaacagct atagacaggt tgtccagttt accctgttct tcttcaatca tttcctttat
     2101 ttcattgact tcatagttta tttttttatg atccttctgg gatgtagagt ccccgcctat
     2161 accggcctcc gggtattggg ttgtattgtt ggtctcacca ggtattccgc cggcgggatt
     2221 attgctcatc ttctctttta aaagctcctg actgataatg atccctgaat cgtccatgac
     2281 cggttcaaaa gttatggatt ctttggttct cttgtcaaag ttcagcttcg cgtttacggc
     2341 ggtctttatc ctcccatatc caaacaccgg ttccaacagg cctacgattt cggcctttat
     2401 tttattttcc aattgctgtt gcattacaaa ctggttagta accgtctggg catcctgggt
     2461 gccctcggga tttagtatgt tcattttgtt gtctatgata gatatattct ctttatccaa
     2521 cccgggtaca ctggttgaca taagctgttc gatgctctta gcctgttgcc cggttatctc
     2581 atatccatgc tttggtaaaa ttattgcaga tgccgataca ggtatttctt cctcttttaa
     2641 tacaaatgaa tccgatttgg gcatagcgat tgtcactact gcatcctcaa ccccctcaag
     2701 gaaacgtatg gactgaccca aacgttcctg aagctgtata atccatcttt ttggatctca
     2761 tcgttggtct ggccaaagga atttccctga aggtagagat catagttaaa cccggatttt
     2821 gggtatcctt ccaaggtcat ttgcatcctc agttccgctt ccttttcttt tgggactaaa
     2881 atagtcgctg taccgtccat cttgggttgt gcatttactt ctttaagctt tgtatagacc
     2941 tcggctgcct ctttctctgt caaacctgta tataacacta cataatcctt tttggctaat
     3001 aggactgaga taaagaccac aaccagcaca attaaaccaa ttataaaaat taaattcctc
     3061 tttttgggtt tatcaaggtt gtcccagtaa ttattcagtt gttgttttat agattccagt
     3121 accctcaaca tcccatccct gcttcccctt aaatgccagg tttctcacca attacccatc
     3181 tgtatctttt gctaaataga actatacgct aaatccgacc ctataaatag catcgttatg
     3241 tatcggacac atcccaaact cagcccaatt atgggatagg gaaaccatcc aaaggaaatg
     3301 gcttttcaac aagcaaatta aagatatcat taaaaatgat gttatacctg catcctcatt
     3361 atttcctggt aggcatccaa tatcttattg cgaatttgta gagtaaattg aagagcgata
     3421 tcggcctttt ccatatcgat cattatcctg tctaaattgt ctacctgtcc cgtagccata
     3481 tgggtattgt taacaatatc ccttttcctg agttcttggg catcttgtgc tacattttca
     3541 agtatatcct taaatttagg ggtattagtt gcggatttgg tccccgcaga ttccgtcaat
     3601 ccggaagggt ttaaatatag attttgaatc ttcatctggt tagccccctt tatctcctgc
     3661 caatctctag cgctttaatc gccatgctct ttgtagcgtt aaaggcggta acattagctt
     3721 caaaagatcg tgtggcggat atcatatcga ccatctcttt taaattatct acatttggca
     3781 tgtgaacata gccctcatcg tcagcatccg gatgtccggg atcaaatatc gtcttaaatg
     3841 gagtcgggtc ctccgtcacg cttgtagctt caactccgtg gcccgtaaaa gtattactgc
     3901 tctctgaaag aaaacgggaa aagggcttgg cctcaatccc ctcttgtaca gatatcaatc
     3961 tgcgcctgta aggcccccca cgggcagttc gggtagtgtt catattggct atgttttgtg
     4021 caataatatc cattctgagc cgttgaaccg ccataccaga agcactaatt tgcatggatt
     4081 ttatcatatg ctaacgtctc ccctcattaa taatactctt aagccttgaa aattctccat
     4141 ttagtttttg gattaaggca tggtgcataa cggtattgga ggcgagattc gccatctcgg
     4201 agtctatatc cacattatta tcatccattc gcatctttgt ggaattaaca tctaccgttt
     4261 ttggcccgat ttcatttatg ggaacgttcc ctatcctaag atgtcctttt ctggtctgtc
     4321 taccctgaat gctgcgccta gtaagggcag acacaagttc attctcaaaa acaacttctt
     4381 ttttcttata tcccggtgtg tccgcatttg atatgttgtg ggaaatgact tcatgccttt
     4441 tccaaactac atccaggctc ctttccaaag cggcggtatg ggaaaatagc gatccattta
     4501 ccatgtattc acccccatat gtggaatatt aatacatata tttatgttgc acaaaaaact
     4561 ccaaaacaaa tccaatatcc attgttttcg ccaattataa tgaaagttct gctttttatc
     4621 tatgtaattt atatattttt atcataatag c
//
LOCUS       DASRNX010000098        33180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_498242,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000098 DASRNX010000000
VERSION     DASRNX010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 33180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33180
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498242"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..860
                     /locus_tag="VFD89_03790"
     CDS             396..860
                     /locus_tag="VFD89_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57343.1"
                     /translation="MYNDDMYSVRYATDPIYNQEAGYDYYGGASEPYYPYPTPCPEYP
                     STPPMPPVHPMPPGPPMPPTMPMHPVDPWSAMKILCLTHLKNMLEKMKDKKVALIIEG
                     ARGNFDCVRIMSVDECKVVAETKNGVCVISLREITAVCMTKEVADQILKDKK"
     gene            901..1053
                     /locus_tag="VFD89_03795"
     CDS             901..1053
                     /locus_tag="VFD89_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57344.1"
                     /translation="MPKIGTYFSIALAMLFSAMAFNAYCSNAHQVPPVHPKCIILIDT
                     IGSGML"
     gene            1201..1680
                     /locus_tag="VFD89_03800"
     CDS             1201..1680
                     /locus_tag="VFD89_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57345.1"
                     /translation="MHREIIRSTKGDRLSILSLSLGLMVLLVAIVGISNFLYNRFGIT
                     YSGYIAFLLYILVGILVYRRRILEYSYSLVGNELVFESLAGKRSREIARVPLDGIIYF
                     CPLTHERKDTNTRYKSHLIAFGRRSAGVYVLAFERDSSVHRIVFQPSQKLIGLINNS"
     gene            2151..2783
                     /gene="tmk"
                     /locus_tag="VFD89_03805"
     CDS             2151..2783
                     /gene="tmk"
                     /locus_tag="VFD89_03805"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011254134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZJ57346.1"
                     /translation="MRGYFITFEGPDGAGKTTQIDLLREYLLGRGVDVVVTREPGGTP
                     ISEAIRDIILDTNHDEMDPLTEAYLYAASRAQHVSQLIIPALRQGKTVLCDRFVDSSI
                     AYQGAGRGLGMKMVEEINRAALRGIMPDITLFFDIDPEVGLVRGRKRDRSADRLELEG
                     IEFHRNVYEGFCMLCGMYPERFRRIEADAGIMEIHHRIINEIGQLPKYGK"
     gene            2921..3250
                     /locus_tag="VFD89_03810"
     CDS             2921..3250
                     /locus_tag="VFD89_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HZJ57347.1"
                     /translation="MKLIIAVVQDEDSQKLISRLMKAGYGVTKLATTGGFLRAGNTTL
                     LIGVDNERLENAMGIIEHVCKSRKQVATSPTPMAGATGMYVPYPVEVTVGGATVFVVN
                     VEEFRKV"
     gene            3247..3705
                     /locus_tag="VFD89_03815"
     CDS             3247..3705
                     /locus_tag="VFD89_03815"
                     /inference="COORDINATES: protein motif:HMM:NF015822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HZJ57348.1"
                     /translation="MRIEKTGNRPEEGHRAGGAKGAGGPIPGSHAKSFDSFLNDSQYK
                     SMNEELILMAEDIKKQGQRLANRTNLGELLRYKKMVAQFLDISVRKMLEFRRKDYLDG
                     SGKHHIYALVQKVDQNLEVLTREILDSQRDQLVVLNCIDDIRGLLMDLIL"
     gene            3932..4942
                     /locus_tag="VFD89_03820"
     CDS             3932..4942
                     /locus_tag="VFD89_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HZJ57349.1"
                     /translation="MNTNNIIGQFTLINGLRAMVDSGRIVHSYIFIGPEGVGKKTISG
                     FFAKMLLCTGVEKPCNRCKSCLQFDSDNQPDLIRVKSDKGIIRVDAIRDVRRDIGIKP
                     FQGLRKIYIIEDGETMNQYAQNAFLKTLEEPPKHAVIMILASNLTSLFPTIVSRCQQI
                     RVPGLSTKEVTRIIERKAQMPREEALVYGKLSQGIPGMGLKLAGSDEYRQLREESLAI
                     LNKLANSSLVESMAYIDYFLDNRDNITEILDIIGLWLRDVLILKQDGCKGVIINSDKL
                     SKLEPLAKNFTIPAIQDIIGNIEDSKMMLRSHANFQLTIENMLMKIQGSGKHAGGSRS
                     TV"
     gene            4914..5798
                     /locus_tag="VFD89_03825"
     CDS             4914..5798
                     /locus_tag="VFD89_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003363049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HZJ57350.1"
                     /translation="MQEVVGVRFKKAGKIYYFNPDSVELNKGQCAIVETSRGIEYGEI
                     VVGPKYVSEEDIVSPLKKVIRRATEEDSQTVRENQTKEIDAFDICVQKIEKHNLPMKL
                     VDVEYTFDNSKAIFYFTADGRVDFRELVKELASIFRTRIELRQIGVRDEAKMIGGLGP
                     CGRPLCCKSFLGEFEPVSIRMAKEQNLSLNPTKISGICGRLMCCLKYEQSYYEEVRKR
                     MPRVNREIITPQGIGTVISSNVLTGRVRAKVIQSDGTPAIFDFALGEIKYLNGKSYED
                     FDEVVDESKGNLAPLLDE"
     gene            5927..6097
                     /locus_tag="VFD89_03830"
     CDS             5927..6097
                     /locus_tag="VFD89_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZJ57351.1"
                     /translation="MAYFINDECIACGVCEAECPTEAISPGDDIYVIDADACIDCGAC
                     ADVCPVDAPQPA"
     gene            6425..8962
                     /locus_tag="VFD89_03835"
     CDS             6425..8962
                     /locus_tag="VFD89_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZJ57352.1"
                     /translation="MFRRFLAIVVGIMVCINLLWIPPIYGAGDTSLIRVKLSSMGSPK
                     SVNVYVDGYYGVPQIDKSNLLTKGNYTVRIESGKLVLDDKTGSKTVSTVLGSGFTFKR
                     YKGADPNHIKVGTFNYQGDMQVRLSGSSIEIINHIHLETYLYGVVPHEVSNSWPIEVQ
                     KAQAVAARTYVMGKRNAGAAYDVVDTTTDQVYRGYNTSYTNSIKAVNDTFGKVLKYGS
                     GYAGTFYSSSNGGMIESSKNLWGTDLAYCRVKEDPYDIKNESNKNRSWTVTYKKKPVD
                     SGLLSRLNFVSALKDKGLKEADIENLEIREFSFDYNSSTRVDKGKIILAMNKKSDKKD
                     IPEETPPEEEDAPEEILITIPLAKANIRSILDLNSLLFKVQAKDNNFIFRGSGWGHGI
                     GMSQYGAQQMAKEGKKYKEILNFYYPGTKLADLGIEADRGGDPRPDPKPDPKPDPKPD
                     PKPDPKPDPKPGDGGKQQLGRVNVSTSLNVRQGAGTGYKKIGSLYKGDKVEILDRSGA
                     WYKVKKGSLQGFVHGDYIVIEDTGQSSPPPTPGTDPNPGTTDPAPPPSDDRRYGIVTA
                     SALNVRSGPSTKNHIVGMVVKGAKLTILGTQGDWYRISLNGKDAYVHGNYVKLETSSG
                     TTPPPSATVIGKATVTASSLNVRNGAGTGNKILGSLAKGKQVELLEKKGAWYKIKYGS
                     GTGYVHGDYLKLSSSNTTNPTSPSTPPSTAVIGKATVTASSLNVRNGAGTGNKILGSL
                     AKGKRVELLEKKGAWYKIKYGSGTGYVHGDYLKLVSGGESDRGTEQRTGTVTASSLNV
                     RTGPGTNYSRVGLLYRGAKVSITGESGAWYKIKSGSVSGYVSKSYIK"
     gene            complement(9048..10034)
                     /locus_tag="VFD89_03840"
     CDS             complement(9048..10034)
                     /locus_tag="VFD89_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZJ57353.1"
                     /translation="MKKVLVGVLAVLLIILVGIGGYVYKIIGALSDADKLLAGDNPAA
                     WDEDPKEFGIPEGAPMEGDTNVINILLLGTDRRNRNENGRSDAIMIATVDKENGQLKL
                     ASLMRDLYVPIPDRKDNRINAAYSFGGPMLAMRTINQNFNLNLTRYVSVDFFGLEEII
                     NALDGVEIDVKQKEIKFINSVINELNELDKQKRTSPHITQAGSQTLNGKQAVAYSRIR
                     KVGDGDSERTERQRRVMVQMFNKMKSINPMKLPDLVASLIPYTDTNITASEIISLGTS
                     VLTIKDKRIYQYRVPAKDTYKSQTIRGMAVYVPDLDTNTELLHEFLYDKVTE"
     gene            10593..11042
                     /locus_tag="VFD89_03845"
     CDS             10593..11042
                     /locus_tag="VFD89_03845"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZJ57354.1"
                     /translation="MDKAKRHAWILPILWMGVIFIFSHQAGEESAGLSGGVTQVILTF
                     AEGIGIRLDEVRFHGIVRVMGHFIIFFVLGQLWYITLRIRGKSPNHASIIAFLLCIGY
                     AFFDELHQYFVPGRACELSDVLVDGAGAGVAIIIGWLTQFSGREKAA"
     gene            11309..11998
                     /locus_tag="VFD89_03850"
     CDS             11309..11998
                     /locus_tag="VFD89_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZJ57355.1"
                     /translation="MDNMLFFITVFLQGVASFLSPCVLPLIPAYLTYMTGQSIDIMLR
                     DRRAHRTLVINSLAFILGFSLVFVLLGIIATSLGRFLQANKDTIRKVSGVLIIFFGLF
                     HTGLIPVRFLNYERRLNIKSGHPGLLSSFLIGVGFSFGWSPCIGPILTSVYIMASQAD
                     TLFIGMMLLVVYAMGLGFPFFLMAAGLKYFWKYIQKLNRHMDKVKIISGVLLVIVGVM
                     IYFNAFYYLIP"
     gene            12077..12682
                     /locus_tag="VFD89_03855"
     CDS             12077..12682
                     /locus_tag="VFD89_03855"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZJ57356.1"
                     /translation="MKKIAKSTLVLVIFLVLILMISACQRNDAKTPDNSDMTLEENVD
                     YVRIDKPSVDFELEDLDGNTIKLSDYKGQLVFLNFWGTWCPPCRKEMPHFESIHKQFG
                     DKGTKILAVSSTQVELQGGNDSDRAKNQVQSFIDDGGFTFPIPLDSGNEVFEEYNKIF
                     PVIGIPITYMIDKEGIIRYVLPGAFRDEDHIKAFMALLDQE"
     gene            12743..13501
                     /locus_tag="VFD89_03860"
     CDS             12743..13501
                     /locus_tag="VFD89_03860"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HZJ57357.1"
                     /translation="MRVEIKEHERVDDLDLKGLKIIQDPNMFCFGMDAVLLSHFAKAR
                     SGERVVDLGTGTGVIPILMSGKSEDTQFYGVEIQASMADMARRSVMLNSLEDRIRIVE
                     GDLKESPNYLGSGEYQLVTANPPYKKMGTGIINPNNAKAVARHELKCNLDDVLKAASG
                     LLAWGGRLSMIHRPERMIEILSGMRLHGLEPKVIRLVYPNLRKPPNMLLIEAVLGGRP
                     HLVWMPPLIVYEPGGEFTSELMRIYYGDKERQAF"
     gene            13576..14427
                     /gene="rsmI"
                     /locus_tag="VFD89_03865"
     CDS             13576..14427
                     /gene="rsmI"
                     /locus_tag="VFD89_03865"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZJ57358.1"
                     /translation="MGKGTLYLCATPIGNLEDITLRVIRALGEADYIIAEDTRHTLKL
                     LNHLNITKPLISYHKYSDMKRAEEILSLLEAGNEIALVSDAGMPGISDPGEDLVRLAY
                     ERDIPVTILPGATAGLSALVLSGLPGGRFVFEGFLPRGKKEAERILALLQSEERTTIL
                     YEAPHRLMGTLNELYKALGDRRISVVRELTKIHEEVLRFSLKEAIEHFSINKPRGEFV
                     LVVEGKEDRGEDNAFDNISIRDHILEYMEAGMVKKAAVRQVAEDRSLPKSQVYRHSID
                     LPLKVGD"
     gene            complement(14590..14832)
                     /locus_tag="VFD89_03870"
     CDS             complement(14590..14832)
                     /locus_tag="VFD89_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZJ57359.1"
                     /translation="MKSTGIVRKVDELGRIVIPIELRRTLNIAEKDALEIYVDGEHII
                     LKKYQPACIFCDDARDVFDYKGKNVCKNCLAELINR"
     gene            15008..15622
                     /locus_tag="VFD89_03875"
     CDS             15008..15622
                     /locus_tag="VFD89_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HZJ57360.1"
                     /translation="MVNVVWILLIFIGFVSSLVFGNLEHSTQSALDGAKHAVELCIGL
                     SGVYALWLGLMKVAEKSGLVEVISKRMKGLMSVLFPGIPPGHRAMGAMTMNIIANMLG
                     LGNAATPFGIKAMHELQSINRYKDTATHDMCMFLIINTSSVQLIPATVIALRSAAGSM
                     NPAEIIGTSLIATTCSTLAGIIAAKMLKGSYTVHKSNDRTGRGV"
     gene            15626..16168
                     /locus_tag="VFD89_03880"
     CDS             15626..16168
                     /locus_tag="VFD89_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HZJ57361.1"
                     /translation="MIEFIRILSVIAIPGFIVLVLGYGHIKRINVYDTFIEGAKEGLS
                     TVIRIFPYMVAMFVAIGIFRSSGAMDILVKVLSPLTGLIGIPGEILPLAFMRPVSGIA
                     SLGILGELYRTHGPDSFIGRLASTVMGSTETIFYTLSIYFGAVGIKRMRYTLWGALLA
                     DLVGLLASVIICTIVFGGGK"
     gene            16398..17129
                     /locus_tag="VFD89_03885"
     CDS             16398..17129
                     /locus_tag="VFD89_03885"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HZJ57362.1"
                     /translation="MKISVYPTCDYVEDRDIKDRIVVVIDVLRATSTIITALYNGCKE
                     IIPTADIEDAVNISKNYEKDTFILGGERNAVKIEGFHLSNSPCEYSREQVENKTVLIT
                     TTNGTKAITRAVDAKEVVLASFINISAVYEYAAAVGKDISIICAGTLGRFSADDVLVA
                     GALIRGLENRGLSLELDDLGMVAKNMYDSSKDDLHGALSNTLHYSILKNLGLQGDLDD
                     CLTLDTAPIVPIYSDGIVRAWHISI"
     gene            17526..19562
                     /locus_tag="VFD89_03890"
     CDS             17526..19562
                     /locus_tag="VFD89_03890"
                     /inference="COORDINATES: protein motif:HMM:NF026221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HZJ57363.1"
                     /translation="MSGKGKWWEDHNWRLVQTNLREIDMADIDAKRYVADLESFNATV
                     VMINTSGIIASYKTKLPFHYQSPYLTGDSLKDIIAECHRAGIRVIARTDFSKVRRQIY
                     EMYPNWAFVDKDGDIVDYGGDVHVCINSDYQQKYAFEIIRETLEELDVDGIFFNMGGY
                     VSFDYSQNYHGICQCLGCQKRFGEMYGLPLPKKEDMDDPVYKKYLLFKQETMDKFHKR
                     LFKFIKSIDPDILINQDTYTEKSGFIRQESNTALDRPLPHWQYSGSENTKWVRGSFPG
                     FISTNTTVDFIDFPTRHVSVSPYQQELRLYQSLANGGAVDYYLIGRLDNHSDKSGYEG
                     IRRAFDFHKDNEDHYKDLHGDCDTVILTEPHWGGQGPGDMAEFFGLYRVLAETHKLFD
                     VMFIPQVKEAGLSKYKNVILPNVDRIDDETARILDDFVAQGNTLLATGKTSFTDGNYE
                     ARDRAALECLGIGSGFVARDARGAYFMVGDDKEFPGFSEHECELVSIDGDYFYVEYEE
                     GAEKHMRLLPPQPYGPPERCYPLYESVDRPAAAVNSFGKGKAIHIPWLPGRYFHRQGY
                     INTFWFLTDVYERLMGIRSIEGNIPPTVEATLLRSRDGGKRIFHLINNNGHYGNSFFK
                     PTPIYDIECRIPVSREPDSVSSLNKGKILDYSWEEGCLTLRLEKLDCFEAVLID"
     gene            19670..20851
                     /locus_tag="VFD89_03895"
     CDS             19670..20851
                     /locus_tag="VFD89_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZJ57364.1"
                     /translation="MERELSQLNPGYRILLGPGPVETSPRVLRAMSANMLGHLDHQFL
                     EIMNDTMELLRRVFQTKNKLTLPMSGTGSAGMETVMVNLIEPGDNAIVCVNGVFGQRM
                     RDVAERCGANVYSVEAPFGQPIDPSDIRKAAEKVGEVRLISVVHAETSTGILQPLPEI
                     SEIAREYDALFVVDAVTSLGGIELKVDDWGIDACYSGTQKALSCPPGLSPVTISPKAE
                     KVIAERKSKVSSWYLDLSMIQNYWGQERVYHHTAPINMIYGLREALQIVMEEGLEGRF
                     KRHKQNSDAFISGIEAMGLSMVAPVGFRLPTLNAVMIPENVDDLKARNHLLEKYGIEI
                     GGGLGDFKGKAWRIGFMGESSRRNNVVLLLAALADAMGLQGHRVDVGAAVEAALKVYN
                     G"
     gene            20946..23078
                     /locus_tag="VFD89_03900"
     CDS             20946..23078
                     /locus_tag="VFD89_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HZJ57365.1"
                     /translation="MSIDSHPWYKRVTRWGQTNLTEDDPARNNIEFWKEQWRRTRVQG
                     VIVNCGGIVTYYKTDFKEQYLASGLGDRDYFKDFLDAATDQGLVVVARMDTNRATKAF
                     YEGHPDWFCVDRDKNPVIRQKRYVACVNSGYFSEYLPALITEIIEKYRPAGFAENSWS
                     GLGKDEICYCDNCRELFLDTAGYGLPEKVDWEDPIYREWIRWNYERRNENWDFLNEIT
                     TRIGGEDCYYCGMIHADPTNPGGRFVDIKELCKRSKIIFTDHQSRDPLNGFEQSYVNG
                     LLLRFAGHEETVVPQSTAYYVRGDRTFRLSAVPPNEPNLWMISGGAGGISPWFHHISG
                     AKYDRRQFEHPIPYYNWHEANEKYLYNRTSLANVGIVWNQTNADFYGRDDVYERVSLP
                     WSGYISALVQWRIPFIPVNAADIGRYSHRIKTLVLPDVAILSDSQIDDLCSFIEGGGN
                     IVITGITATLDGDGLPSKTKRLWEILGIEFTGETEGAFRRQPSDWEYPHAHTYIHLPE
                     ERHELLTGFEETDIIGFGGGLNIVKSHGSLRSISGYIMPFPIFPPEFSWIREVREDLP
                     TVLAGKLATGSRVVYFPADIDRCFGRERLPDHGRLLANAVQWAAGDTFTLKVESKGLI
                     IVHPYRQEDRYLLHLVNLSGCNQNPGYCTETYPIEDVKVRMPVDSGIEKVRLLAGQKE
                     IPFEVSDGHVIFTIDRIEEFELAVLEGK"
     misc_binding    23254..23488
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            23730..25742
                     /gene="metG"
                     /locus_tag="VFD89_03905"
     CDS             23730..25742
                     /gene="metG"
                     /locus_tag="VFD89_03905"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZJ57366.1"
                     /translation="MKDNKTFYITTPIYYPSDKLHIGHSYTTVAADAMARFKRLTGYD
                     VMFLTGTDEHGQKIEQVARENGVSPKDYVDHIVSGIKDLWKTLDISYDHFIRTTDPGH
                     QKIVQRIFKKLYDQGDIYKSEYEGWYCTPCESFWTQIQLAEGKCPDCEREVELVKEES
                     YFFKMSKYQDRLIKYIEEHDEFIQPHSRKNEMLNNFLRPGLEDLCVSRTSFKWGVPVT
                     FDEGHVIYVWIDALSNYITALGYLTHDDEKYEKYWPADVHLVGKEIVRFHTIIWPIML
                     MALGEELPKQVFGHGWLILEGGKMSKSKGNVVDPVILEKRYGLDALRYFLLREVPFGS
                     DGVFSNEALVNRINSDLANDLGNLLSRTVAMIDKYFDGIIPSPGEPENVDRELVHMAL
                     KAPKKVEELMDELLFSNALTEIWKLVGRTNKYIDETTPWVLAKSQEHRPRLGTVLYNL
                     AESLRIISTLISPFMPGTPGEIRHQLGIDDIEGLKAWESLGSFGKIPAGTRVCKGEPI
                     FPRLDVKKELEELEKNLPTQPKTKTHGGYGDQNRGDEKRMETGNDEKKGETQGMSEIT
                     IDDFAKLDLRVARVIEAEKVEKSDKLLKLQLQVGEEVKQVVSGIAEHYSAEELVGKDL
                     ILVANLKPVKLRGNLSQGMILAASDKDGKLALVTIDSQMESGSSVS"
     gene            25753..26523
                     /locus_tag="VFD89_03910"
     CDS             25753..26523
                     /locus_tag="VFD89_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZJ57367.1"
                     /translation="MLFDTHAHLDDEKFDDDRDELILGLPDRGVLRVICPGIDVESSK
                     KCIELSEKYEIIYAGVGIHPHEAGSADGDYLDSLRSLSGRKKVVAIGEIGLDYYYDFS
                     PRDVQKRIFAEQIELANEAGLPVIVHNRESHRDVLDILRSYKELLIGGVMHCYSGSWE
                     MAKLFLDLGLYISLGGPVTFKNAKRPVEIAENIPLDRLLIETDSPYLTPVPHRGKRND
                     PGLVGFVAERIAEIKDIPADEVRRITFENAAMLFKTEG"
     gene            complement(26689..28758)
                     /locus_tag="VFD89_03915"
     CDS             complement(26689..28758)
                     /locus_tag="VFD89_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZJ57368.1"
                     /translation="MYTLKSIRRLIKKCLPVLLIMTLLAGCQDVNPMEAAKTYTDAWA
                     DLDFVKMYETLSAESRATISEEDFVERYKTIFSAIELNSIDLTPQEMLDEGDNAYLPV
                     DAVFDTETVNSFETRFTLPLVLEDEQWRVDWTPALIFPGLDREDQVRITGQHAHRGNI
                     VDREDNPIAIDGEAYTVGGVPGKIPDRDEFARKLSPLLELSEEKILDELSKDWVRDDT
                     LVPLRNFPLSISAEFKDEILSVKGVMLVTNLVTDSRRYLNNELYSHIVGYVQRVSAED
                     LEEKPGYRDRDLIGRQGIEASMEEHLHGLSGYSLFIRDTDGNKKSTLAEVAARDGNTI
                     GLTLDPDLQGTVHKALQGHVGSVTVLNGKTGEVLAMVSYPDYNPNIFPNGVLPSSWEA
                     LTEHPDKPFINRSTYALYPPGSTIKPFSAAMALEEGVINPDTVVEAAQKRSWTPDLDD
                     WRAPPIIRTNHPEGPVNLDKALVWSDNIFFAWTALRTSYETMESYAARFGIGEPMDFD
                     VPVSRSILKRQDTNWSKHLLATSAVGQGEVLVTPLQMAAMYTAFLNQGDMLLPKIVKD
                     IRSPEGEILEQLEREVWQSSAIPQRLIDTILPSLVGVVEDEGGTARSLAIDGLTIAAK
                     TGTAEKDGKSDEDKEEIAWLAAFTTDSANPLIVMVALEVPAGEGRDKFDIVREVFMDY
                     YGISDEE"
     gene            29338..30510
                     /locus_tag="VFD89_03920"
     CDS             29338..30510
                     /locus_tag="VFD89_03920"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HZJ57369.1"
                     /translation="MEPQQQNMRKNRLLLYVCIVLVIVGGTTTALAHYIQEAHYDVTI
                     DDNGYIAQIQTMDETVKELLDKYEINLGPGDEITPGLEEPIGKDTEIKIRRAFKVTVE
                     ADGKMKTVHMIRGTVGDALDKAYVVLGEKDLISDSPDQQASPGDHIRVTRVKEEVLTE
                     KETIPYKVVTKENDKLNKGTEKLVQEGKEGLKEREILIAYHDGHEVNRELVEERVSEE
                     PIDRVIDKGTYVAPPPASRGDVKREKVIAVKSAKEKTAKGKTQKKKVVETKPTKSKTT
                     KANTSKKKQSTKKAYLSAGTYTFQATAYIRTGNRTNSGKWPKEGMIAVDKRLIPLGTK
                     VHVEFPGKWAHLSGYYTAEDTGGAIKGKIIDVFMDTDDESRADKFGRVNNIKVTIP"
     gene            30593..31471
                     /gene="rsmA"
                     /locus_tag="VFD89_03925"
     CDS             30593..31471
                     /gene="rsmA"
                     /locus_tag="VFD89_03925"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZJ57370.1"
                     /translation="MSQLTSLKVIRDILQRHNFRLSKSLGQNFLADRNIVEKIIDGAE
                     VTEKDMILEIGPGIGTLTKDLMARAGKVVAVEIDRDLFPVLEETLGAPPNLTLIHGDI
                     LKTDIKRLVDKEFGGNEFKVVANLPYYITTPIIMRFLEEELPVTSLTVMIQREVARRI
                     TAGPGEKEYGSLSVAVQYYTNPRVLCNVPATVFMPRPKVDSMVVILERRNRPAIEVRD
                     RQLFFRIVRGAFAKRRKILLNNLTTGEFGEWDRERMLEILHMSGIDPKRRGETLTLEE
                     FAGISNVMATISVDKQ"
     gene            31649..33154
                     /locus_tag="VFD89_03930"
     CDS             31649..33154
                     /locus_tag="VFD89_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57371.1"
                     /translation="MARKSYYHRSFIILKPESRGYGSIPDKAPTGYCKFEIRRGIGKA
                     YIYIQDIKSSSSLDGAYEAYLISADDSIRPCKLAGLYADEQGRCEHIVSFDADDIKGS
                     GYTLESFHALVIAYRTGTEGMGIAYPLIGYAGKDIYMSTQRITAGLKALYGEEIELLS
                     APEPRHEPSDYQEAGGPAEIEVADRKEAEYIEKADPTTDGDEGVGSRPGTETGPAEEE
                     TIEEAAEEEAIEEKAVEEEAIEEEAAEEGLPAWEEPGIGPAAESDSGPETEIITEGLP
                     QSEAPAEDPSRHQIGIEPELQPADAFMPDINLEPLTIYPGRSSPPPDEGTGQQTGSGE
                     QPEEEQQGKSEEQLRRAYEDSYQEYLQDLAYEDKTYPHYGATYWDNVKDYFIGLFKAH
                     QRVFPFGEGQDDIEWIRVQQATAGSSGYFAESSYGYPSYNYNYPDHHIVGIAKIEGRV
                     QYVVYGMPSMYSMIPPISINGFSRWVPIKGGFGMGYWLLYIDAISGRIVYP"
BASE COUNT         9438 a         6775 c         8598 g         8369 t
ORIGIN      
        1 gtagcgttgt gcccttaaca gggctatacg gtagaggcga actggacacg cctgtacgta
       61 tcaaagtagc ttagctgcta tgaacgtaaa tgagttctta tgaactctag aaccccgtga
      121 ctttagccat gggaggttca gtcaccggcc cctaaatggg attaggatat agttcaataa
      181 aggatagcag gattatgacg gaaatcgtac cctgctttct ttttttgcct atgtaacagt
      241 ttaagggttg gaacatagaa tatatcaagg tcaaaaatat ggttagcaat atatgatata
      301 gagtaaaatg gctatccatc aaatcaacca agcccttatg aaaacaataa atatggttgt
      361 agacttggct gattatagga attggaggta tcaagatgta caatgacgat atgtattctg
      421 taaggtatgc tactgaccca atttataacc aggaagccgg ctatgattac tatggcgggg
      481 cgtcagagcc gtattatccc taccccacac cgtgtcctga atacccatca acaccgccca
      541 tgccgcccgt tcacccgatg ccaccggggc cgcctatgcc gcccacgatg ccaatgcacc
      601 ccgtagatcc atggtcggcc atgaagatac tctgtctgac ccatctcaag aatatgcttg
      661 agaagatgaa ggataagaag gtagcactta ttatagaagg cgccaggggc aactttgatt
      721 gcgtcaggat aatgagcgtt gacgagtgta aggttgtagc cgagacaaaa aatggggttt
      781 gcgtaatttc ccttagggaa ataacggcgg tatgcatgac taaagaagta gccgaccaaa
      841 ttttaaagga caagaagtag gggatattcg ggctacttaa cgtttaggga tattaaccat
      901 ttgccgaaaa tcgggaccta tttcagcatc gccttggcga tgcttttttc tgccatggca
      961 tttaacgcct attgcagcaa tgcccaccag gttccccctg tacaccccaa atgtattatc
     1021 ttaattgata ccatcggctc gggtatgcta taatgttcta tatctataga taagcgataa
     1081 caaaactaaa tttatagata tgaaagttgc aacgggtcta tgcaatttca tggcgataaa
     1141 ctttatagtt gaaattgttt atttgcaaca ttaaacaaag cggttagagg ggatgcatgt
     1201 atgcacaggg agattataag gtccactaag ggagaccgat tatccatatt aagcctatct
     1261 ttaggcctga tggtgttgtt ggttgccatt gtcgggatct ccaatttttt atataacagg
     1321 ttcggaataa cctatagcgg ttatattgcc tttcttttat acatacttgt gggcatactt
     1381 gtgtatcgga gaagaatatt ggagtacagc tattccctgg tagggaacga actggtcttt
     1441 gaaagtctgg cagggaaacg gagccgtgag attgccaggg tgccccttga cgggatcata
     1501 tacttttgtc ccttgaccca tgaaaggaag gatacaaata ccagatacaa gagccacctt
     1561 attgctttcg gtagacgatc tgcaggggtc tatgtattgg cctttgaaag ggacagcagt
     1621 gttcatagaa tagtctttca gccgagccag aaactcatcg gtctaattaa taattcatag
     1681 ggtacttgca ctttatagcc ttatccggaa taaaatagta aaagccggca atcctttaga
     1741 tagtcgataa atagtatttg gataaggcgg tgcgatgatg atccgtataa ttgaatgttg
     1801 tgtgtgcaag agaatgacca gggccaatct cctccttagg ggtaccaaca tatgccttga
     1861 atgtgaaaaa aagattacga atcttggaag taaccatgag gactaccccg ggttaaaaga
     1921 gggaataaag cggatgctgt ggcgtgatca actggtatcc agaccgtgat atgtacactt
     1981 acgctttatg gggatgatgg tgttgacatg catttgttgt acaagcgtat aagacaatta
     2041 tataccgata attggtccga ctaaaagagg cttttgtggg gtatcgggcc ggattatttt
     2101 aaagactgga acatgtatga taaagatagt ttttatggga gtggataggg atgaggggct
     2161 attttattac ctttgaaggt cctgatgggg cgggcaagac cacacagata gacctgttaa
     2221 gggagtattt actgggcagg ggcgtggatg tggtggtcac ccgagaaccg ggaggtaccc
     2281 ccatcagcga ggccataagg gatattatcc tggatacgaa ccatgacgag atggaccccc
     2341 tcacagaggc gtatctatac gccgcgtccc gggcccagca tgtaagccaa ttgataattc
     2401 ctgcgcttag acaaggcaag acggtgctct gcgataggtt tgttgattcc agcatcgctt
     2461 atcagggggc cggcaggggg cttggaatga agatggtgga ggaaatcaac agggccgcac
     2521 ttagggggat catgcccgat ataaccttat ttttcgatat agaccctgag gtggggttag
     2581 tcagggggcg caaaagggac cggagcgcgg atagactgga actggagggt atcgagtttc
     2641 accggaatgt gtacgagggc ttctgtatgt tatgcggtat gtaccccgaa aggtttaggc
     2701 gtatcgaagc ggatgcaggt ataatggaaa tacatcatcg gataattaat gaaattgggc
     2761 aattgcctaa gtatggaaaa taggatataa tacatataga ggtataagat gattattgtc
     2821 tagagagtat tatagtcctg ccgggcttac tttacgggtg gtaatcatat agtggtaact
     2881 atattttagc gtcacaaaaa aagaaaagga ggtatttccg gtgaaattaa ttattgccgt
     2941 agttcaggac gaagattccc aaaagcttat ttcgaggctc atgaaggcag ggtatggagt
     3001 caccaagctg gcaactacag ggggttttct aagagcggga aacacgacat tgcttattgg
     3061 tgtcgacaac gaaaggctgg aaaatgccat gggaattatc gagcatgtat gcaagagccg
     3121 taagcaggta gctacatcac cgacacccat ggcaggtgca accggtatgt acgttcctta
     3181 tcccgttgaa gtaacggtgg ggggcgctac agtttttgta gtgaacgttg aggagttcag
     3241 gaaggtatga gaattgaaaa gaccggcaac cgccccgagg aaggccatag ggctggaggg
     3301 gcaaagggag ccgggggccc gattccgggc tcccatgcta aatcctttga cagcttccta
     3361 aacgacagcc agtataaatc catgaacgaa gaattgattt taatggcgga ggacatcaaa
     3421 aagcagggcc aaaggctggc taacagaaca aatttggggg aactgttgcg ttacaaaaaa
     3481 atggttgccc agtttctgga tatatccgtc agaaagatgc tggagtttag gaggaaggat
     3541 tatctggacg gtagcgggaa gcaccacatt tatgctctag tacaaaaggt agatcaaaat
     3601 cttgaagtcc tgactaggga gatattggat tctcaaaggg atcagctggt ggtgctaaat
     3661 tgtatagatg atattcgtgg gctgttaatg gatttgattc tctagtctgg agagagcgga
     3721 aataaagcaa aatacaataa ccaagaatgt tttatctttt atgggcgaag tagtgggtat
     3781 tttgatatag gagaattaga taaaggggta attagcaggg gcgttagtta taagagatta
     3841 caattattgc aaccgcgaaa aggttattta gcagaaagga gattcggcgt ttcctcccat
     3901 gactaaagtc acgggaattc acgtcaaaat tatgaataca aataacatta ttggacaatt
     3961 cacccttatc aacgggctga gggccatggt tgatagcggg aggattgttc attcttatat
     4021 attcatagga cccgagggtg tcggcaagaa gaccatatcc ggtttttttg ccaagatgct
     4081 cctgtgcacc ggtgtggaaa agccatgtaa ccggtgtaaa tcctgcctgc aattcgattc
     4141 ggacaatcaa ccggatctga tccgcgtcaa gagtgacaag ggcattatca gggttgatgc
     4201 cattagggat gtgagaaggg atatagggat caaacccttt cagggcttaa gaaagatata
     4261 tatcatagaa gatggggaaa ccatgaacca atatgcacag aatgcatttt taaagaccct
     4321 ggaggagcct ccaaaacatg ccgtcatcat gatattggcg agcaatttaa ccagtttgtt
     4381 tccaaccatc gtatccaggt gccaacagat tagggtgccc gggctgtcca ccaaggaggt
     4441 cacgcggata atcgaaagaa aagcacaaat gcctcgggaa gaggcccttg tgtatggaaa
     4501 gctgtcgcag ggaattccgg gcatggggtt gaagctggct ggatccgatg aatatcggca
     4561 gcttagagag gaatctctgg caatattgaa taagttggca aatagttcct tggtggaatc
     4621 catggcttat atagattact ttttagataa tagggataat attactgaaa tactggacat
     4681 aatcgggctt tggctcaggg acgttctgat actgaagcaa gacggttgca agggcgtaat
     4741 aattaactcg gataaattat ccaaactgga acccctggcg aaaaacttta caatccctgc
     4801 tatacaagat attataggga atatagaaga cagtaaaatg atgttaagat cccatgcaaa
     4861 ctttcaattg accattgaaa atatgctgat gaaaattcaa ggaagtggta agcatgcagg
     4921 aggtagtagg agtacggttt aaaaaagccg gtaaaatata ttatttcaac cccgacagtg
     4981 ttgaacttaa caaagggcaa tgtgctatag tcgaaacctc caggggtatc gaatacgggg
     5041 agatcgtagt gggccctaag tatgtctcag aagaggatat cgtatcccca ctaaagaagg
     5101 tcatcaggcg ggctacggaa gaggattctc aaactgtgag ggaaaatcag acaaaggaaa
     5161 tagatgcctt tgatatatgt gtacaaaaga tagaaaaaca taacctgccc atgaagctgg
     5221 tggatgttga atataccttc gataatagca aggctatttt ctacttcacg gcagatggca
     5281 gggtagattt tagagagctg gtcaaggaac tcgcctccat attcaggacc cggatagaat
     5341 taaggcagat aggggtaagg gacgaagcca agatgatagg gggactgggc ccttgcggaa
     5401 ggccgctgtg ctgtaagagc tttttgggcg aatttgagcc ggtatctata aggatggcca
     5461 aggaacagaa tctgtcccta aatcctacca agatatcggg tatatgcggc cgactcatgt
     5521 gctgcctaaa gtatgaacag agctattacg aagaggtacg aaagcgaatg cccagggtga
     5581 acagggagat cattacaccg caaggcattg ggacggtaat cagttccaat gtactcaccg
     5641 gaagggtcag ggcgaaggtg attcaaagcg acggtacccc ggctatattt gattttgctc
     5701 ttggggagat aaagtattta aacggaaaga gctacgagga ttttgatgag gttgtggacg
     5761 aatcgaaggg caacttggcc cctttactgg atgaatagcc gtataagata ccgcatgtta
     5821 aaagtttgtt aaaaggcctt gctttttttg cccggggtgg tagaataata agggtgttaa
     5881 aagtgacacc ttatgttagg ggtttaaggc gaggaggtga cttgatatgg cttattttat
     5941 caatgatgag tgtattgcat gcggtgtctg tgaggcagaa tgcccgacag aggctatatc
     6001 accgggggat gacatctatg taattgatgc agacgcatgc attgattgtg gggcttgtgc
     6061 agatgtctgc ccagtggacg caccccaacc ggcataatac atagggaatt tatagatgat
     6121 ttttgtgtac aggctgttaa caaattaacg ttaacagctt ttcttttcgt gacgatactg
     6181 agcgttcaat agccattacc gacatatttg acgggcatag tgcattaatt caatatagaa
     6241 taaggcgttg aaattaatat gggataaagg tttttcgtgc aacacatata gtagaaaagt
     6301 cccatattat attgatgggg tattttttca tggtttgggg aggaattcgt aaaaaaatag
     6361 cgaatgaata tagtgtggta catattatgt atagtagaaa ttatgttaaa aagggtggtt
     6421 tggaatgttt aggaggtttc tggcaattgt agtaggcatc atggtttgta tcaacctatt
     6481 atggattccg ccgatatatg gggcaggtga taccagcctg attcgggtaa aattgtcatc
     6541 tatgggttcg cccaagagcg taaatgtcta tgtagacggt tactatggtg tgccccaaat
     6601 tgacaagagc aaccttctaa ctaagggtaa ctacacagtg cgtatcgaat caggaaaatt
     6661 ggtattagac gacaagaccg gctctaaaac ggtatcaacg gttttaggca gcggctttac
     6721 ctttaaacga tataaggggg cagaccccaa ccatatcaag gtgggaacct ttaactacca
     6781 gggggatatg caggtacgcc tgagcggcag ttccattgaa attataaacc atatccattt
     6841 ggaaacctat ctttatggcg tggtgcccca tgaggtaagc aacagctggc caatcgaggt
     6901 ccaaaaggcg caggctgtgg cggcccgtac atatgtcatg ggtaaaagaa atgcaggcgc
     6961 cgcctatgat gtggttgata caaccaccga tcaggtgtat aggggctata atacgagcta
     7021 tacaaattca atcaaagccg ttaatgatac cttcgggaag gtcctaaaat acggtagcgg
     7081 ctatgccggt actttttatt cctccagtaa cggcgggatg atcgagtcct caaagaactt
     7141 atggggaacg gacttggcct attgtcgtgt aaaggaagac ccctacgata tcaaaaatga
     7201 aagcaataaa aatcgtagct ggactgtcac ctataagaaa aagcccgttg attcgggtct
     7261 tctgtccagg ctgaactttg taagtgccct aaaggataag ggcctgaagg aagcggatat
     7321 agaaaacttg gagattagag aatttagttt tgattacaac agcagtacca gggtcgataa
     7381 gggaaagatt atcctggcga tgaataaaaa atcggataaa aaggatatcc ccgaggagac
     7441 tcccccggaa gaggaagatg ctccggagga gatactcatt actatccctc tggccaaggc
     7501 caatattcgc tccatccttg acctaaacag tcttttattt aaggtgcagg ccaaggataa
     7561 caactttatc tttaggggca gcggctgggg ccatggtata ggtatgagcc aatatggcgc
     7621 acaacagatg gccaaggaag ggaaaaaata taaggaaatt ctaaactttt attatcccgg
     7681 caccaagctt gccgatcttg gtatcgaagc ggataggggt ggggatccaa gaccggatcc
     7741 aaagccagac ccgaaaccgg atccaaagcc ggatccgaaa ccagacccga aaccggatcc
     7801 caagcccggc gacggaggca agcagcaact tggaagggtt aatgtatcca ccagcctcaa
     7861 tgtacggcag ggagcgggaa cgggttacaa gaaaatagga agcctctata aaggggataa
     7921 agttgagata ttggatcgaa gcggcgcctg gtacaaggtt aaaaagggta gccttcaggg
     7981 atttgtccat ggcgattata ttgtaataga ggatacgggg caatcatctc caccgcccac
     8041 gcctgggacg gatccaaacc cgggtacaac ggatccggca ccgccacctt cggatgacag
     8101 gcgctatgga atagtaactg catcggcact taacgtaagg agtggaccca gcacaaagaa
     8161 tcatattgtg ggcatggtgg tcaaaggagc gaaacttact atactgggaa cccaggggga
     8221 ttggtacagg ataagcttaa acggtaagga tgcctatgta cacggaaatt atgtcaaact
     8281 tgagacttct tccggcacaa cgccaccacc gtctgccact gtgataggta aggccacggt
     8341 aacggcatcc agcttaaacg taagaaatgg agccggcacc ggcaacaaga tcctggggag
     8401 cctggccaag ggcaaacagg ttgaactatt ggagaagaag ggcgcttggt acaagataaa
     8461 gtacggcagc ggaacgggat atgtccatgg ggattactta aagcttagca gttcaaatac
     8521 cacaaatccc acatccccgt caacaccacc gtctactgca gtgataggca aggctacggt
     8581 aacggcatcc agcttaaacg taagaaatgg ggccggcacc ggcaacaaga tcctggggag
     8641 cctggccaag ggcaaacggg ttgaactgct ggagaagaag ggcgcttggt acaagataaa
     8701 gtacggaagc ggaacaggtt acgtacacgg agattatttg aaactcgtat ccggcggtga
     8761 aagcgacaga ggaacagaac aacgtaccgg gaccgtaact gcatcgtcat tgaatgtaag
     8821 gaccggtccg gggacaaact attcgagggt aggattgttg tatcgcgggg ccaaggtatc
     8881 cattacaggc gaaagtggcg catggtacaa gattaagagc ggctctgtaa gcgggtatgt
     8941 atcgaaatcc tatatcaagt gattatgaac agctaaaata taaaggcttc tgcagcggga
     9001 aaaccgttgc agaagccttt atcctatgat gcgtctattt gatacgatta ttctgtaact
     9061 ttatcatata aaaattcatg taatagttcc gtattggtgt ccagatccgg tacatatacc
     9121 gccatgcccc ttatggtctg gcttttatag gtatccttgg ccggaaccct gtactgataa
     9181 attcttttat cctttatggt caataccgag gtccctaggc ttatgatctc ggatgctgta
     9241 atattggtat cggtataggg aatgagggag gctaccagat caggtaactt cattggattg
     9301 atggatttca tcttgttgaa catctggacc attacccttc tctgtctttc ggtacgttcc
     9361 gaatcaccgt cgccaacctt tcttattctg gaataagcta cagcctgctt gccgtttaga
     9421 gtctgggacc ccgcttgggt tatatgtggg gaggttctct tttgtttatc caattcgttt
     9481 agctcattta tgacggaatt gataaatttg atttccttct gctttacatc gatttcaacg
     9541 ccgtctaagg cattaataat ctcttccaga ccaaagaaat ccacactcac gtagcgggtt
     9601 agatttagat taaagttttg attgatagtt cgcatggcca gcataggacc tccaaaggaa
     9661 taggcggcat tgattctgtt atcctttctg tcgggtatgg gcacgtacag atccctcata
     9721 agggaagcca attttaattg gccgttttcc ttgtccactg tggctatcat gatggcgtct
     9781 gatcggccat tctcattgcg gttgcgtctg tcggtaccca gtaaaagaat atttatgacg
     9841 ttggtatccc cctccatagg agccccttcc ggtataccga actctttggg atcttcgtcc
     9901 caagcggcgg gattatcccc ggctaagagt ttgtccgcat ccgataaagc ccctataatt
     9961 ttatatacat agcctccaat tcccaccagg attatcagca aaacagccaa tacccccact
    10021 aacacttttt tcatatcaat tcctccattt agcctaacgt tgtattcatt aattaatcat
    10081 tatcaacccg atgatttcag aatcatatgt ttcaacggga cgagttactc ttattcacca
    10141 cctatatcta atgaaaatag gctcgccata tacataggca ggggtatttg ccgtcccgtt
    10201 atattattct acaaaaacct acacccgtca atagattgta atacacttaa taatatgatt
    10261 ttcatccata aagcttgtta gccttggaaa ctaagcgatt gggatattat tcttcaaatt
    10321 ttaattatac acccaatata atatgtatat ctgacatggg gtgtcttctg ggatcttctt
    10381 tagagtatgc atccggcggg aaaaaggtgt tgttggcatt agaggggtta tatggatctg
    10441 ctgcgcgaaa actaaaaatt atcttagcga taattgaaat gggcttacgc aatttcatta
    10501 ttgcaaattt tatagttgaa gttgtttacc tataaaacca gtatttgtgt tataatttca
    10561 caaaagcggt ttggaaggtt gagataaatt tgatggataa ggctaagaga cacgcttgga
    10621 tattgcctat actttggatg ggggttatat ttatattctc ccatcaggcc ggggaagaat
    10681 cggcgggact cagcggggga gtgacgcagg ttatattaac cttcgcggaa gggataggta
    10741 tcaggctgga tgaggtgcgg tttcacggta ttgtacgggt gatggggcat tttatcatat
    10801 tcttcgtatt gggacaattg tggtatataa cgctccgaat ccggggtaaa tcgcctaatc
    10861 atgcgtccat aatcgcattt ttactctgta tagggtacgc tttttttgat gaattgcacc
    10921 aatattttgt ccccggtagg gcctgcgaac ttagcgatgt tctggtagac ggcgcagggg
    10981 cgggggtcgc aattataatc ggatggctga cacagttctc agggagggaa aaagctgctt
    11041 aaagtccgtg tttataagat gggggatagg gtattacttt atatatggat aaacaatagg
    11101 tcaactaaat ttatcggtat gaaaattgca atgattctgt gcattttcat ggtgataaat
    11161 ttcatatata tgttatgaat aagacctacc atgtgatgca attgccaatg caattgcttt
    11221 cgatgtttta acgcgggaag tttatcgtaa aacatatagt tggagttgtt tatctatata
    11281 acgccgtttt tccgggaggg gtaagtgcat ggataatatg ctgtttttta ttactgtttt
    11341 tttacaaggg gtggcatcct ttctgtcccc ttgcgtattg cctttgatac cggcttatct
    11401 tacctatatg accgggcaat ccatagatat aatgttaaga gataggaggg cccaccggac
    11461 gctggtaatt aattcattgg cctttatctt gggattctcc ttggtttttg ttctgcttgg
    11521 cataatagcc acttcattgg gaagattttt gcaggctaat aaggatacca tcaggaaggt
    11581 cagcggggtt cttataatat tctttggact gtttcatacc ggacttatac ctgtgagatt
    11641 cctaaactac gaaagaagat tgaatataaa aagtggtcat cccggccttt tatcctcatt
    11701 tttaataggg gtggggttta gttttggatg gtccccctgc attggaccca tattgacgtc
    11761 ggtttatata atggccagcc aggctgatac cctatttatc gggatgatgc tgctggttgt
    11821 atatgctatg ggcctgggat ttcccttctt tcttatggcg gcgggactga aatacttttg
    11881 gaaatacatc caaaagctga acaggcatat ggataaggta aagataataa gcggggtttt
    11941 gctcgtaata gtaggcgtaa tgatctactt taatgccttc tattatttaa ttccataact
    12001 tggatgccct acggcagata ccttatttta gctttttatc gcatattaaa agagtcagaa
    12061 agagtaggtg tacaagatga agaaaatcgc gaaatcgaca ttggtcctgg tcatatttct
    12121 cgttcttata ttgatgatat ccgcatgtca gcgaaatgat gcaaagacgc ccgacaatag
    12181 cgacatgacc ctggaagaga atgtggatta tgttaggata gataagccat ccgtggactt
    12241 cgaattggag gatctggatg gaaacaccat aaagctctcc gattataagg ggcagttggt
    12301 cttcttaaat ttttggggta cttggtgtcc cccctgtaga aaggagatgc cccatttcga
    12361 gtcgatccat aagcaatttg gagacaaggg cacaaaaata ttggctgtca gttctaccca
    12421 ggtggaatta caggggggga atgatagcga tagggccaag aaccaagtcc aatcctttat
    12481 agacgacggc ggctttacct ttcccattcc gctggattcg ggcaacgagg tatttgaaga
    12541 gtataataaa atatttcccg tgatagggat tcccataacc tatatgattg acaaggaagg
    12601 tattatccgc tatgttttac ccggggcctt tcgagacgag gatcatatca aggcgtttat
    12661 ggcgttgttg gatcaggaat aatacccgga gtaaactata aagcccttgg gcccgggcct
    12721 taattaaagg atgataatga atatgagagt cgaaataaag gaacatgaaa gggtagatga
    12781 tctggacctg aagggactaa aaattattca ggaccccaac atgttctgct tcggtatgga
    12841 tgcggtgctc ctgtcccact ttgccaaggc acgatccggc gaaagggtgg tggatttggg
    12901 caccggaacc ggggttatac ccatattgat gtcgggaaaa agcgaggata cccaatttta
    12961 tggggtggaa atacaggctt ccatggcaga tatggccagg cgcagcgtta tgctgaacag
    13021 cctggaggat agaattcgga ttgtagaggg ggacctaaag gagtccccta attatttggg
    13081 gtcgggtgaa taccagctgg tgaccgccaa tccgccatat aaaaagatgg gtaccggtat
    13141 aataaatccc aataatgcca aggctgtggc ccgtcatgaa ctcaaatgta acttagacga
    13201 tgtcttaaag gcagcttccg gcttgttggc ttggggtgga aggctatcca tgatccacag
    13261 gccggagaga atgattgaga ttctgtccgg tatgagactc cacggtctgg aacccaaagt
    13321 aatccgcctg gtctacccta atctgagaaa gccccccaac atgctgctca tcgaggctgt
    13381 cttaggggga agaccgcacc ttgtatggat gccccccctc atagtatacg aacccggcgg
    13441 ggaatttacc tccgaactta tgcggatata ttacggggat aaagagcggc aagcctttta
    13501 aaggggacgg actatagctt tctggacagg cattgaacga ttaaaatatc gggaattgga
    13561 gggattgatg acataatggg taaagggaca ttatatctat gcgctacacc catcggcaac
    13621 ttagaagata taaccctaag ggttataagg gccttagggg aagccgacta tatcatagcc
    13681 gaggatactc gacacacatt aaaactgtta aaccacctta acatcaccaa gccccttatc
    13741 agctatcata aatacagcga tatgaaaagg gcggaagaga tactctccct attggaggcg
    13801 ggcaacgaga tcgcgttggt atccgatgcg ggcatgcccg gaatttcgga tcccggagag
    13861 gatctggtaa ggctggccta cgaacgggat attcccgtta ccatattgcc cggagccact
    13921 gccggcttgt ctgcactagt gctttccggg ttgccggggg gcagatttgt atttgagggg
    13981 tttttgccca gagggaaaaa ggaagcggaa aggatcctcg ccttgctcca atccgaagag
    14041 cgcactacta tattatatga agccccccac cggctgatgg gcacactgaa cgaactgtac
    14101 aaggcgctgg gggacaggag gatctctgtt gtaagggagc tgaccaaaat ccatgaagag
    14161 gtcctgcggt tttcattaaa ggaggccata gagcatttta gtattaataa gcccaggggc
    14221 gaatttgtac tggtggtaga ggggaaggag gatcggggag aggataacgc atttgataat
    14281 atctccataa gggatcatat actggagtat atggaagcgg gtatggttaa aaaagcggct
    14341 gttagacagg tggccgaaga ccgcagccta cctaaaagcc aagtataccg acattccata
    14401 gacctccctt taaaggtcgg ggattgatta ggctcggctt agataaaaaa gaaaaaaggc
    14461 gactcataat ttatcgcctc tttcgcatca ttaatcattt aggcaatcgc catggcaatt
    14521 gcagcatatt aatataatag tcaaaatcag gtgaaattgt tctggtgcgc atcggcgccg
    14581 aggagcaatt tacctgttga ttaattcagc taaacagttc ttgcaaacgt tcttgccttt
    14641 ataatcaaag acatcccttg catcatcaca gaaaatacat gcaggctggt actttttaag
    14701 aatgatatgc tcaccatcta cgtaaatttc cagggcgtcc ttttctgcga tatttagagt
    14761 tcttctcagt tcgatgggta tgactattct tcctagctcg tctacttttc tgacaattcc
    14821 tgtggacttc attagctttt cccccctcta cttacaatat tcgacaaaga cccattggta
    14881 tattaccaat aattccaatt aaagtcaacc ctttttttaa ccatttttta gaatttgtgt
    14941 tctatgggtg ctatccgttc ataagtttta atagtaggag tattgcaaaa gaaatggggg
    15001 gccataattg gtaaatgtag tatggattct gctgatcttc ataggctttg tatcctcatt
    15061 ggtattcggc aacctagagc atagcactca atcggctttg gatggggcta agcatgcagt
    15121 ggaattatgc attgggttat cgggggtgta cgccctgtgg ttgggactca tgaaggttgc
    15181 cgaaaaatca ggattggtgg aggtcatatc taaaaggatg aagggactga tgtccgtatt
    15241 atttcccggc ataccccccg gacatagggc tatgggggcc atgaccatga atatcatcgc
    15301 caatatgtta gggcttggga atgcggctac cccttttggt ataaaggcaa tgcatgaact
    15361 tcagagcata aacaggtata aggatacagc tacccatgat atgtgcatgt ttctgattat
    15421 aaatacttcg tcggtccagc tgatccctgc taccgtgatt gctcttcgaa gtgccgccgg
    15481 ttctatgaat cccgcggaaa tcataggcac cagcctcata gccactacct gttctaccct
    15541 ggccggaatt attgccgcaa agatgctaaa ggggagctat acggtccata aaagcaacga
    15601 tcgcaccgga cggggggtct gatttatgat agaatttata agaatattgt ctgttattgc
    15661 tatacccggg tttatagttt tagtactggg ttacgggcac ataaagagga taaacgtata
    15721 cgacaccttt attgaaggtg caaaggaggg gctatccacg gtgatacgga tatttccgta
    15781 tatggtggca atgttcgtag ccatagggat ctttcgatct tcgggagcta tggatattct
    15841 ggtaaaggtg ttgtccccgc ttacggggct tataggaata cccggggaaa ttctgcccct
    15901 ggcctttatg agaccggtat ccggcatagc atcactgggc attctgggcg agctgtacag
    15961 gacccatggc cctgattctt ttatagggag acttgcttcc accgtgatgg ggtctaccga
    16021 aaccatattc tataccctat ctatatattt tggagccgta ggcataaaga ggatgcgcta
    16081 tacactgtgg ggagcgctat tagcggattt ggtcggtctg ctggcttctg tgataatatg
    16141 taccatcgta tttggagggg gaaagtagca ccatttatat ggaatttgtc gacttatagg
    16201 tgggcttatg ctaatatata tagataaaca atacgaaaac taaatttatc ggcatgagaa
    16261 ttgcaagggt tttatgcaat ttcatggtgt taaattttat agttgtagtt gtttatccat
    16321 atgtgatgca attgtcgatg ctgttgcttc cggtattata aagggtatta aacacggact
    16381 tttacgagga ggactttatg aagattagtg tatatccaac ttgcgattat gttgaggata
    16441 gggatatcaa agatagaatc gtggtagtta tagacgtgct cagggcgacc agtaccatca
    16501 ttaccgcctt gtacaacggc tgcaaggaaa ttatacccac cgctgatata gaggatgccg
    16561 taaatatctc caagaactat gaaaaggata catttatcct aggcggtgaa agaaatgccg
    16621 taaagataga gggctttcat ctctccaact ccccctgcga atacagcagg gaacaggttg
    16681 aaaacaaaac cgttcttatt actaccacca acgggaccaa ggctatcacc agggctgtgg
    16741 atgccaagga agtggtactg gcatcgttta tcaatatctc tgccgtatac gaatatgctg
    16801 ccgctgtggg caaggatatt tcaattatct gcgcaggtac cctgggaagg ttttcggcgg
    16861 atgatgtact tgtagcaggg gccttgataa ggggtttgga aaacaggggt ctatccttag
    16921 agttggatga tctgggaatg gtcgcgaaaa acatgtacga cagttcgaag gatgatctgc
    16981 atggggcgct atctaatacc ctacactatt cgatactcaa gaatctgggg ctccaagggg
    17041 atttagacga ctgcttaacg ctggataccg ctcctatcgt gcccatatat tccgacggga
    17101 tcgtgagggc ttggcatata agtatatgat aaaaaggctg ctcatttagc agcttttgtt
    17161 atttgtgggg gattatgcag atcggagaaa aatatatagc gacgaaaaat tgaaacctga
    17221 tgaaatatcc tatacaatag caatatatat aatttgattt gatgcggtgt aaccatataa
    17281 tccaatggga tgtgcaggtt catcaagtac cgattaaaca tataggggaa ccggcacaac
    17341 aggaaattta gcatttaact atatatgtta cgaaaaagac ctttcattgt aaaatcggct
    17401 aagttatccg attttgcgtt aaaataatgt atttttcgta acatacgaat taaggataat
    17461 gttttacgac acctgtgttc caatgtagaa tgtttgccgt aaaacaaata gggaggagac
    17521 tatatatgag cggtaagggt aaatggtggg aggatcataa ctggcgactt gtacaaacca
    17581 atttgaggga aatcgatatg gccgatatag acgcaaagcg atacgtggcc gacctggaat
    17641 ccttcaatgc gactgtcgtc atgataaata catccggcat catagcgagc tataagacga
    17701 aattgccttt tcactatcaa agtccatatc ttacggggga cagccttaaa gatattatag
    17761 cagagtgtca tagggcgggg ataagggtta tagccagaac cgatttttcc aaggtcagaa
    17821 ggcaaattta tgagatgtat ccaaattggg cctttgtaga taaggatggg gacatcgtag
    17881 attacggcgg ggatgtccat gtatgcataa acagcgatta ccaacaaaaa tatgcttttg
    17941 agattatacg ggagaccctg gaggagctgg acgtagacgg cattttcttc aacatggggg
    18001 gctatgtatc ctttgattac agccaaaact accatggtat ctgccagtgc ctaggttgcc
    18061 aaaaaagatt tggcgaaatg tatggtctgc cccttcccaa aaaggaggat atggacgatc
    18121 ccgtgtacaa gaaatacctc ttatttaaac aggaaaccat ggataaattt cataaaaggc
    18181 tgttcaagtt tataaaatcc atagaccccg atattttgat aaaccaggac acatacacgg
    18241 agaagagcgg tttcattcgc caggaatcca atacggccct ggatcgcccc ttgccccatt
    18301 ggcaatacag cggttctgag aacaccaagt gggtccgcgg ctcgtttccc ggatttattt
    18361 ccaccaacac cactgtggac tttatagact ttcccacaag gcatgtttca gtatcgccgt
    18421 atcagcagga attgaggctg taccagagcc ttgccaacgg cggggccgta gactactatc
    18481 tgataggcag attggataac cacagcgata aatccggcta cgagggaata cgtcgggcgt
    18541 ttgacttcca caaggacaac gaagatcatt ataaggatct gcacggggat tgtgacaccg
    18601 ttatattaac tgaaccccat tggggaggtc agggcccggg ggatatggcg gaatttttcg
    18661 gactgtatcg tgtgcttgcg gagacccaca agctttttga tgtgatgttc attccccagg
    18721 ttaaggaggc gggcctgtca aagtataaga acgtcatcct gcccaacgtt gaccgaatag
    18781 acgatgaaac ggcaaggatc ttagacgatt ttgttgctca agggaacacc ctgcttgcta
    18841 ccggtaagac ctcatttacc gacggaaatt atgaagcccg ggatagggcc gcactggaat
    18901 gtctggggat agggtccggg tttgtagcaa gagatgcccg aggtgcctac tttatggtcg
    18961 gagatgacaa agaatttccc ggattttcgg agcatgaatg cgaactggta agcatcgatg
    19021 gggattactt ctatgtggaa tacgaagagg gcgcagaaaa acacatgaga ctgcttccgc
    19081 cgcagccata cggaccgccg gagcgctgtt accccctcta tgagagcgta gatcgtcctg
    19141 ccgcggctgt gaattccttt ggaaaaggaa aggcgatcca cataccgtgg ttgccgggca
    19201 gatacttcca tcgccaggga tatatcaata cgttttggtt tttgaccgat gtctatgaaa
    19261 ggcttatggg catacggtct atagaaggga atattccccc taccgtagag gcgacccttt
    19321 taaggagtag agacggcggg aaacgaatat ttcaccttat caacaacaac ggtcattatg
    19381 gaaacagctt ttttaaaccc acccctatat atgatattga atgcaggata cccgtttcca
    19441 gggagccgga ttcggtatcc agcctaaaca aaggcaagat cttggattat tcttgggagg
    19501 agggctgcct gaccctcagg ctggagaaac tggattgctt tgaggcagta ttgatagatt
    19561 aaagagttgt gccggtggaa tatgttttgg taatggctgt tgacaagaga atggctgcac
    19621 tgaaatgatt gcactgaact gaagaaaatc acaatttgga gggattttaa tggaaaggga
    19681 attaagtcaa ttaaaccccg ggtataggat tttgttaggg ccggggcccg tggaaacgtc
    19741 ccccagggta ctgagggcaa tgtctgccaa tatgcttggg catttggatc atcagttcct
    19801 ggaaatcatg aatgacacca tggagctgct tagaagggta tttcagacaa agaacaagct
    19861 gaccctgcct atgtcgggaa ccgggagcgc agggatggaa accgtgatgg tcaacctcat
    19921 tgagccaggg gacaatgcca ttgtctgcgt aaatggggta ttcggacaga ggatgcgcga
    19981 tgtagcggaa agatgcggag ccaatgtcta cagcgttgaa gcacccttcg gacagcccat
    20041 agacccctcg gatataagga aagcggcgga gaaggtcggg gaagttagac tcatttcggt
    20101 agtccatgcg gagacctcaa cgggtatctt acagcccctt cccgagattt ctgaaatagc
    20161 cagggaatac gacgcattgt tcgttgtgga tgctgtgacc tccctgggcg ggatagagct
    20221 taaagtggac gattggggga tagatgcctg ttatagcgga acgcaaaagg ctctcagctg
    20281 tcctccggga ttatccccgg tgaccataag ccctaaggca gaaaaggtca tagccgaaag
    20341 gaagtccaaa gtaagcagct ggtatttgga tctcagcatg atacaaaact attgggggca
    20401 ggagcgggta taccaccata cagctcccat aaatatgatc tacggcctta gagaggcgct
    20461 gcagatagta atggaagagg gacttgaggg gagattcaaa aggcataagc aaaattccga
    20521 tgcctttata agcggtatag aggccatggg tctttccatg gtagcccccg tcggatttag
    20581 attgcctacc ctaaatgccg taatgatacc cgagaacgtg gacgacctta aagccagaaa
    20641 tcatcttcta gaaaagtacg gtatagagat cggcggagga ttgggagatt ttaagggaaa
    20701 ggcatggagg ataggattta tgggcgaatc ctcaagaaga aacaacgtag tattgctctt
    20761 ggccgcattg gctgacgcca tgggcttgca agggcatagg gtggatgtcg gcgcggctgt
    20821 tgaagctgct ctgaaggtat ataacggtta aatattttga gctatagaag tattggagaa
    20881 tgcttggata aagcattctc tctttataaa atgaaattca gatcgatggg gggacgtatg
    20941 agcatatgag cattgattct catccgtggt acaagagggt aacaagatgg gggcagacca
    21001 atctgacgga ggacgaccct gcgcggaata atattgaatt ttggaaagag cagtggcgga
    21061 gaacccgcgt ccaaggcgtc attgtcaact gcggcggtat cgttacgtat tataagacgg
    21121 attttaaaga gcaatactta gcatcggggc tcggtgacag ggattatttt aaggattttt
    21181 tagacgccgc caccgaccag gggcttgtgg tcgtagcccg aatggatacc aacagggcca
    21241 ccaaggcttt ttatgagggc caccccgatt ggttttgcgt tgatagggac aaaaaccccg
    21301 tcatccgcca aaagcggtac gtcgcatgcg tcaacagcgg ttactttagc gaatatcttc
    21361 ctgccctaat caccgagatc atagagaagt acaggccggc cggttttgca gaaaacagct
    21421 ggagcggcct tggaaaagat gaaatctgct attgtgataa ttgcagggaa ttgtttttag
    21481 atactgctgg atacggactc cccgaaaagg ttgactggga agatcccata taccgggaat
    21541 ggatacgatg gaactatgag cgcagaaacg agaactggga ctttctaaac gaaattacca
    21601 ccaggatagg gggtgaggac tgctattatt gcggtatgat ccatgccgat cccaccaatc
    21661 ccgggggaag gtttgtggat ataaaggagc tctgcaagag gtctaaaata atatttaccg
    21721 accaccaatc tcgggatccc ctaaatgggt tcgaacagag ttacgtaaat gggctgctcc
    21781 tacggtttgc cggtcatgaa gagactgtag taccccaatc tacggcatat tatgttcgcg
    21841 gcgacaggac ctttcgatta agtgccgtcc cgcccaacga gcctaacctg tggatgattt
    21901 ccggcggcgc cggcggtatt tcgccttggt ttcaccatat aagcggcgct aaatatgaca
    21961 ggcggcaatt tgagcatccg ataccgtatt acaattggca tgaagccaat gaaaaatacc
    22021 tgtataatcg tacttccctg gcaaacgtgg ggattgtatg gaaccaaacc aatgcggatt
    22081 tctatggcag agatgatgta tacgagcggg tttccctgcc ctggagcggt tatatatctg
    22141 cgctggtaca atggcgtata ccatttatcc ccgttaacgc cgccgatatc gggaggtatt
    22201 cacatcggat aaagacactg gttttacccg atgtagccat tctcagcgac agccagattg
    22261 atgatctttg cagctttatt gaaggcggag gcaacatagt gataaccggt attaccgcta
    22321 ccttagacgg cgacggtctg ccgtctaaga ccaagaggct gtgggagata ctgggcatag
    22381 agttcaccgg agagacggag ggcgcatttc gcagacagcc cagtgactgg gagtatcccc
    22441 atgcccatac ctatatccat ctccccgagg agcggcatga actacttacg ggatttgagg
    22501 agaccgacat catcgggttt gggggcgggc tcaacattgt aaaaagccat ggttccctca
    22561 ggtccatcag cggctatata atgccatttc cgatttttcc tcccgaattc agctggatca
    22621 gggaagtccg tgaggacttg ccgaccgtcc ttgcgggaaa gctggccacg ggctctcggg
    22681 ttgtatactt ccctgccgat atagacagat gcttcggccg ggaaaggttg ccggatcacg
    22741 gaaggctcct cgcaaacgcc gttcaatggg cagcggggga taccttcacc ctaaaggtgg
    22801 agtctaaagg gctcattatc gtccatccat accggcagga ggaccgctac ctgttgcatc
    22861 tggtgaacct aagcgggtgt aaccaaaatc ctgggtattg taccgaaacc tatcccatag
    22921 aggacgttaa ggtcaggatg cccgttgatt ctggtataga aaaggtcagg ctcctagcgg
    22981 gccaaaagga aatacccttc gaggtttctg acggacatgt gatatttacg attgaccgca
    23041 tcgaagaatt tgagctggcg gtgttggagg gcaaatgatc ttaaaaccgg aaacgcgtac
    23101 ggggcagatc attttccaga ggttacggga tatggtccgc cggataaaat ttaattaaaa
    23161 tcccgccaag attgttgaca ttagcgggta tattgactat attgttataa aggttaatct
    23221 tcacatcaga accattaatg atttgatgat accccaaacg ctatgaaggg aagagtagaa
    23281 caagggaatc gtttaaaaga gagccgggcg ggtgaaagcc ggcagcgaag aatttgtttg
    23341 aagatggccc cggagcggag gtgctgaagt atgagtaggc agctacggat gatttcgtta
    23401 ttgaatccgg cgatgatggt cgcttattga ggcctttttt aaaggcgaat tagggtggta
    23461 ccgcgatgga atacccctcg tccctatatg gaacgagggg ttttttatat ggtgggatca
    23521 tccgttacgc caagtggggt taatcgtatg gacaacgacc tttaggggat atgctatagg
    23581 tatgacagcc tttgtaatga aaggtctttt tactaataca tgtggcatag cccatagaat
    23641 cgggagccat agaccgtcgt tgtagaaccg atgattattt taagttgttg caaaatcgag
    23701 aattatttta attagaaagg atgataagga tgaaggataa taaaaccttt tatatcacca
    23761 cccccatata ttatcccagc gacaagctgc atatagggca ttcctatacc accgtggcag
    23821 ccgatgccat ggcccgtttc aagcgattga cgggctatga tgtgatgttt ttgacgggta
    23881 ccgacgaaca cggacaaaag atcgagcagg tagcaaggga aaatggggtt tcccccaagg
    23941 attatgtgga ccatatcgtc tctggaatca aggacctatg gaagaccctg gacatatcct
    24001 acgatcattt tatcaggact acggacccgg gacatcagaa aatagttcaa aggatcttca
    24061 aaaagctcta tgaccaaggg gatatctata agagcgaata cgagggctgg tattgtacgc
    24121 cctgcgagtc attttggacc cagatccaat tggcggaggg caaatgtccc gattgtgaga
    24181 gggaagtgga actggtcaag gaagagagct acttctttaa gatgtcaaag tatcaggaca
    24241 ggcttataaa atatatcgaa gaacacgacg agtttatcca gccccattcc aggaaaaatg
    24301 agatgctcaa caactttctg cgtcccgggc tggaggatct gtgcgtttcc agaacctcct
    24361 tcaaatgggg cgtgcccgtc acctttgacg agggccacgt tatctatgta tggatagatg
    24421 ccctttccaa ctatataacg gccctaggct acttgaccca tgacgacgag aagtatgaaa
    24481 aatactggcc cgccgacgtc catttggtgg gaaaggaaat agtcaggttc cacaccatca
    24541 tttggcccat tatgctcatg gctttgggag aggaacttcc taaacaggtc ttcggccatg
    24601 gctggttgat actggagggg ggcaagatgt caaagtccaa gggtaatgtc gtagatcccg
    24661 taatcctgga gaagcgatat ggtctggatg cgctccgcta ttttctcctc agggaggttc
    24721 ccttcggttc cgacggggtc ttttccaacg aggcattggt aaatagaata aattccgatc
    24781 tggccaacga tctgggcaac cttctaagcc gtaccgtggc catgatagac aaatactttg
    24841 acggaattat cccctccccg ggggagccgg agaatgtgga tagggaactg gttcatatgg
    24901 cccttaaagc ccctaagaag gtagaagagc tcatggatga gctgttgttc agcaatgccc
    24961 taaccgagat ctggaaattg gtcggcagga ccaacaagta tatcgatgag actacgcctt
    25021 gggtcttagc aaaatcacag gaacacaggc caaggcttgg gacggtacta tataatttag
    25081 ccgagtctct aagaatcata tccaccttaa taagcccctt tatgcccgga acacccgggg
    25141 agataaggca tcagctgggt attgacgata tcgaagggct aaaggcatgg gagagtctcg
    25201 ggagctttgg taaaatacct gcgggtacaa gggtttgcaa aggagaacct atctttccca
    25261 gactggatgt aaagaaggag ttggaggaat tggaaaagaa tctcccaaca caaccaaaga
    25321 cgaagaccca tgggggttat ggagaccaaa accgcgggga tgaaaagagg atggaaacag
    25381 gaaatgatga gaagaaaggg gaaacacaag gaatgtcaga gattaccata gacgattttg
    25441 caaaactgga ccttagggtt gccagagtca tagaagcaga aaaggtagag aaatccgata
    25501 agctgttaaa gctccagctt caggtaggtg aggaggtaaa gcaggtggta tcgggcatag
    25561 cagagcatta ttcggcagag gagttggtgg gcaaagacct catactggtg gcaaacctaa
    25621 agcccgtcaa gctccgtggt aacctctccc agggtatgat attggcagca tccgataagg
    25681 acggcaaatt ggccctggtg accatagaca gccaaatgga atccggatct tctgtaagct
    25741 gaggtgatga aaatgctgtt tgatacccat gcccatctag atgatgagaa atttgacgat
    25801 gatagggacg aattgatcct ggggctcccg gacagggggg tcttacgggt catctgtccc
    25861 ggaatcgatg tagaatcatc taaaaaatgc atagagcttt cggaaaaata tgagattatt
    25921 tatgcggggg tgggcattca cccccatgag gccgggagtg cagacgggga ttatttagat
    25981 tctctccgct cgttatcggg gcgtaaaaag gtggtggcca taggggagat agggttggat
    26041 tattactatg atttttcccc cagagatgtc cagaagcgaa tattcgcaga gcagatagag
    26101 ctggcgaacg aggcggggtt acccgtcata gttcataacc gggaatccca cagggatgtc
    26161 ctagatatcc taaggtctta taaagagctg ttgattgggg gagtaatgca ttgctattcg
    26221 ggtagctggg agatggccaa gctctttcta gatctgggcc tttatatatc cctagggggc
    26281 cccgtgacct ttaaaaatgc caaacgtccc gtggagattg cagaaaacat tcccctggac
    26341 aggttgttaa ttgaaacgga cagcccctat ttgaccccgg tgccccatag gggtaagcga
    26401 aacgatcccg gtcttgtagg ctttgtggcc gaaaggatcg cagagattaa ggacataccg
    26461 gcggatgagg taaggaggat aaccttcgaa aatgccgcca tgctgttcaa aacggaagga
    26521 taggtacagt tcttatgctt cctgccatat aacgagcggc aaggtgcccc ccggctcgct
    26581 gaaacctgtg tgtagcaatt gcttccgatg ttttaatgaa attagatccg tattttaaaa
    26641 tcgcttcccc ccggctgaac gccgggtcgg aagcgatagt ttttttgatc attcttcgtc
    26701 cgatatcccg taatagtcca taaacacttc tcttactatg tcaaatttat ccctgccctc
    26761 gcctgccggg acttcaaggg caaccattac aattagagga tttgcagaat ccgtagtaaa
    26821 ggcggcaagc caggctattt cttccttgtc ctcgtcactc ttgccgtcct tttcggctgt
    26881 acccgtcttg gcggctatgg ttaggccgtc tatggccagg ctgcgggccg ttcccccctc
    26941 atcctctact acccctacca gggagggcaa aatggtatcg atcaatcttt gggggatggc
    27001 cgaggattgc cacacctccc tttccaattg ttccagtatc tccccttccg gagaccttat
    27061 atccttgaca attttgggga gcagcatatc cccttgattt aaaaaggccg tatacatagc
    27121 tgccatctgc agcggtgtta ccaagacctc gccttgtccg acggcggaag tggccagcag
    27181 atgcttggac caattggtat cctgcctctt taaaatggaa cgcgaaacgg gcacatcaaa
    27241 atccatgggc tcacctattc caaacctggc ggcataggat tccatggtct cataggatgt
    27301 tctgagggcg gtccacgcaa agaagatgtt atccgaccat actagggctt tatccaggtt
    27361 taccggacct tccgggtggt tggttcgaat aatgggcggt gccctccagt cgtcaagatc
    27421 cggggtccac gagcgctttt gcgccgcttc caccacggtg tcggggttta taaccccttc
    27481 ttccagggcc attgcagcgc taaagggctt tatggtagac cccgggggat atagtgcata
    27541 ggtagaacga tttataaagg gcttatccgg gtgctccgtc agcgcttccc atgaagaggg
    27601 aaggacgccg ttagggaata tgtttgggtt gtaatcggga tagctgacca tggccagtac
    27661 ctccccggtc ttcccgttta atacggttac cgatcccaca tggccttgta aagccttatg
    27721 gaccgtcccc tgaaggtccg gatccagggt caggccgatg gtattaccgt cccttgccgc
    27781 gacctcggca agggtggatt ttttattgcc gtctgtatcc cttataaaga ggctatagcc
    27841 agaaagaccg tgaagatgct cttccataga ggcttcaatg ccctgcctgc cgatgagatc
    27901 cctgtcccgg tagcccggct tctcctccag atcctcggcg cttacccgct gtacatatcc
    27961 gactatgtga gaatacagtt cattatttag atatcgccgc gaatccgtga caaggtttgt
    28021 gaccagcata acccccttta ccgataaaat ctcgtctttg aattctgcgc tgatactcag
    28081 cggaaagttc ctcaagggta ccagggtatc atcccgcacc caatcctttg agagctcgtc
    28141 taatatcttc tcctcgctca attccaggag cggtgacagc ttcctggcga attcatcccg
    28201 atccgggatt ttgcccggaa ctccccccac cgtataggcc tcaccgtcga ttgcgatggg
    28261 gttatcctcc cgatctacga tatttccccg atgggcatgt tggccggtta tccttacctg
    28321 gtcctcccgg tccaaaccgg gaaatataag agcgggtgtc caatctaccc tccactgctc
    28381 gtcctccaat accaaaggca gggtgaaccg ggtttcgaag gaattaaccg tctctgtatc
    28441 gaagaccgca tcaacgggaa ggtaggcatt atccccctcg tctaacattt cctgcggggt
    28501 taaatcaata ctgttgagtt caattgcaga aaatatggtt ttgtatcttt ctacaaaatc
    28561 ctcctcggat atggtcgccc gactttctgc cgatagggtt tcatacattt ttacaaagtc
    28621 aagatcagcc catgcatccg tataggtttt tgcagcttcc atgggattca cgtcctgaca
    28681 tcccgccaaa agggtcatga ttaataatac aggcaggcac ttctttatta atcgacgaat
    28741 tgatttcaag gtgtacataa tttaaaaata ttccccccta aaaagtcttc cgatatgaat
    28801 tatatcacat tatttatggt attgtatcgg ttcgttgcct ttccattgga taaagataac
    28861 gggagattga caagggaata ccggaggaag ggaagtatca agaaacacat atttattagc
    28921 atctttcgga gcaatattga tcaaattttg aaatttcaat cataggataa cacttaagac
    28981 ggcgtcggcg ttcgatatgt tacgaaaaag acctttcaat gtaaaatcgg caaaactatc
    29041 ctattttacg ttaaaataat gtatttttcg taacatacga attaaggata atgttttacg
    29101 acacctatgt accaatgtag aatgtttgtc gtaaaacata tagatagccg ttattgatat
    29161 atgtcacctg atactggtca tcatttgccc aaccttcggg aggtataaaa taccaaaata
    29221 tcgggtaagg taattttggt acaagcctaa ttattacaaa agcttgacaa gatattaaaa
    29281 tagtattaca atgtccccat caactccctt ggaaaagatt ttaaaggagg aagtacaatg
    29341 gaaccacaac aacagaacat gcgtaaaaac agactgctat tgtatgtttg catcgttctg
    29401 gtaattgtcg ggggtacaac gacggcactt gcccattata ttcaggaagc gcattacgat
    29461 gtcaccatag atgataatgg gtatattgca caaattcaaa ctatggatga aaccgtgaaa
    29521 gaacttttag ataaatatga gataaactta ggtccggggg atgagataac cccgggtctg
    29581 gaagagccca tagggaagga tactgaaatc aaaatccgac gcgcctttaa ggttacggtg
    29641 gaagcggacg gtaagatgaa aaccgtacat atgatccgtg gaaccgtagg ggacgcatta
    29701 gacaaagcct acgtggtctt aggggaaaag gatcttataa gtgactcccc cgaccagcag
    29761 gcatcccccg gggatcatat cagagtaacc agggtgaagg aagaggtttt gacagagaag
    29821 gagactatac cttacaaggt agtcaccaag gaaaacgata aactgaataa gggaacagaa
    29881 aagctggttc aagaggggaa agaaggatta aaggagaggg aaatacttat agcctatcac
    29941 gacggtcacg aggtcaacag agagcttgtg gaagagcggg tgtcagagga accgatagac
    30001 agggttatag ataagggaac ctatgttgca ccaccaccgg cttctagagg ggatgttaaa
    30061 agggaaaaag tgatcgccgt caaatccgct aaagagaaaa ccgccaaagg gaagacccaa
    30121 aagaaaaaag tagttgaaac aaaacctaca aagagcaaga cgactaaagc caatacttca
    30181 aagaaaaagc aaagtaccaa gaaggcttat ctcagcgcag gcacctatac ttttcaggca
    30241 acggcttata tccgcaccgg aaatagaacc aacagcggga aatggcccaa agaaggcatg
    30301 atcgcagtcg ataagaggct gatacctttg ggcaccaagg tccatgtgga atttcccggc
    30361 aaatgggccc acctcagcgg gtattatact gctgaagaca cgggaggggc cataaagggg
    30421 aagatcatcg atgtatttat ggataccgac gacgaatcaa gggcggataa atttggcagg
    30481 gtaaacaata taaaggtaac cataccctga ggattttgtg ttaaactata tatgttacgg
    30541 ataaaggtaa tggcaggata ttatatatcc tgccttttct gggagggagt acatgagtca
    30601 gcttacatct ttaaaggtta tacgggatat tttacaaagg cataacttta ggctcagtaa
    30661 aagtctcggc caaaactttc tcgcggacag gaatatagta gaaaagatta tcgacggggc
    30721 agaggtcaca gaaaaagata tgatactgga aattggcccc ggcataggga ccctgactaa
    30781 ggaccttatg gcgcgggcag gcaaggttgt tgccgtagaa atcgacaggg acctttttcc
    30841 cgtcttggaa gagaccttag gggcaccgcc caatttgacc ctgatccacg gcgacatatt
    30901 aaagacagac ataaaaaggc tggtagataa ggagtttgga ggcaacgaat ttaaggtagt
    30961 cgccaattta ccctactaca taaccacccc tattataatg cggtttttgg aggaggagct
    31021 gcccgtcacc tcattgacgg tgatgattca gcgggaagtg gcccgtagga ttacggcggg
    31081 acccggggaa aaggaatacg gttcgttatc ggtagcggtt cagtactata ctaatcctag
    31141 ggtgctatgt aatgtgccgg cgacggtttt tatgccccgg cccaaggtgg actccatggt
    31201 ggtcatcctt gaaaggcgga acaggcctgc aattgaggtc cgtgacaggc aattgttttt
    31261 ccggattgta aggggggcct ttgctaaacg gaggaagatc ctgctaaaca acttaaccac
    31321 cggcgaattt ggggaatggg atagggagag gatgctggaa attctccata tgtccgggat
    31381 cgatcctaaa aggcgggggg agacccttac cttagaggaa ttcgccggca tatccaatgt
    31441 tatggcgaca atatctgtag ataaacaata ggttagctaa atttatcggc gtgatagtcg
    31501 caatggttct gtgtaatttc atgcggataa attttatagt cggagtcgtc cgtctgtaaa
    31561 aaggaaaccc aataaggaaa aaaagggttt ctttttttat cccccggctc atatactagt
    31621 acaaacaata aaaggggggg aaggaaagtt ggccagaaaa tcttattacc atcgttcctt
    31681 cattatttta aaacccgaga gcagggggta tgggtctatt cctgataagg ctccaacggg
    31741 ttattgtaaa tttgagatca ggaggggaat tggcaaggcg tatatctaca tacaggatat
    31801 taagtccagc agcagcctag atggtgcata cgaggcgtac ctgatatccg ccgatgattc
    31861 tataaggccg tgcaagctgg ccggcctcta cgcagacgag cagggcaggt gcgagcacat
    31921 agtctccttc gacgccgatg atattaaagg ttcgggatat accctggaaa gctttcatgc
    31981 ccttgtcata gcctatagaa cggggacaga gggaatggga attgcatatc ctttaatagg
    32041 atacgccggt aaggatatct acatgagcac ccagagaatt accgcagggt taaaggcgtt
    32101 gtatggagag gaaatagaac ttttatccgc acctgaaccc cggcacgaac cgagtgacta
    32161 tcaggaagcg gggggacccg cagaaataga agtcgcagac agaaaagaag ccgaatatat
    32221 agagaaagcg gatccgacta ccgatgggga tgagggagtg ggatcccgac cggggacgga
    32281 gacaggaccc gcagaggaag aaacaataga agaagcggcg gaggaagaag caatagagga
    32341 aaaggcagta gaggaagaag caatagagga agaggcggca gaagaaggtc tcccggcatg
    32401 ggaagaaccg ggaataggcc cggcggcgga atcggattcc ggaccggaaa ccgagataat
    32461 aacggagggt ctgccgcaat ctgaagcccc ggcagaagat ccgagccggc accagattgg
    32521 aattgagccc gaactccagc ccgcagacgc cttcatgccc gatatcaatt tagaaccttt
    32581 gaccatctac cccggcagat cctcgccccc gcctgatgag ggtacagggc agcaaacggg
    32641 gtccggcgag caaccggagg aggagcagca gggcaagtcc gaggagcagt taagacgggc
    32701 ttacgaggac agttatcagg agtacttgca agacctggcc tatgaggaca aaacctatcc
    32761 ccattacggg gctacctatt gggataacgt gaaggattac tttataggcc tctttaaggc
    32821 tcatcagagg gtgtttccct ttggggaggg ccaggatgat atcgaatgga taagggtgca
    32881 gcaggccact gccggctcca gcggttattt tgccgaatca tcctatggtt atcccagtta
    32941 taattataac tacccggatc accacatcgt gggtatagct aagatcgagg gtcgagtcca
    33001 atatgtggta tatgggatgc ccagcatgta cagcatgatc ccgcctatat ccataaacgg
    33061 attctcccgg tgggtgccga taaaaggggg atttgggatg gggtattggc tgctatatat
    33121 agacgcaata agcggaagaa tagtataccc ctgaaaaaag aatgatcgaa tgatcattct
//
LOCUS       DASRNX010000099         4361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_657198,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000099 DASRNX010000000
VERSION     DASRNX010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657198"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..124)
                     /locus_tag="VFD89_03935"
     tRNA            complement(33..124)
                     /locus_tag="VFD89_03935"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(87..89),aa:Ser,seq:gga)
     gene            401..1327
                     /locus_tag="VFD89_03940"
     CDS             401..1327
                     /locus_tag="VFD89_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZJ57372.1"
                     /translation="MSYNRFDLIAGDGTVITVHKWGLEEGTIASGVVQIAHGMAEWAY
                     RYDYFAKALNRGGYIVYANDHRGHGLTALKPDDIGYISDNDGFIDMVEDMRRLNILIQ
                     EEYPTLPIVLFGHSMGSFLSQRFIQLYGSDLSGVILSGSNGKQGPIINLGITLAYMEM
                     KIRGRRHKSRLLDNLSFGTYNKAFAPNRTKFDWLSRDEEQVDRYMEDPYCGGIFTSSF
                     FYDFLRGLKNITKMKNLKDVPKDLPIYIFSGSMDPVGGFSRGVERLVSMYKNLGLKRV
                     SRKLYPKGRHEMLKEINKDEVIGDIIEWLNEI"
     gene            1784..>4361
                     /locus_tag="VFD89_03945"
     CDS             1784..>4361
                     /locus_tag="VFD89_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZJ57373.1"
                     /translation="MLSKLSVKRPYTVVVAVIMVLILGGISFVNLKTDLLPNIDLPYA
                     LIMTSYPGASPEEVEMVVTKPVEQVVATVSNIKNVSSVSRENSSMVILEFNNDVNMDS
                     ATIEINGMLDLIKPAWGDSIGSPMVMRLNPDMLPIMIASVDVEGMDIIGISQMVNQDI
                     IPELESITGVASVSGVGLLEEKIEVLIDGNKIDSLNKSILDAVDSELSEAEDKLMDAK
                     NEIEDGKAKLESEEKKQGARLSQGEKALSDAKQQIAQGEVQLVSGELELEKVQDELKT
                     KLNEINEKEKELRALAGIIDGLKKNLPQMNEGDLRETLLTLVENLPQEMKERIGAQLS
                     ELEKRLLEGGNLKLQEIEQIIAGISEELNMGLELIMAGKAEIEGGLEQISHNRRELAE
                     QKALLSAKKDELTSKEQEITAGKLLLTMEMDKAKSKLESGEETLNEKMEEFESAKEEA
                     FKKASLDGVITREMISGILAAQNFSMPAGSVSEEGSDYLVKVGDKIHDIEELENLLLF
                     DTGDKGIGKIYLKDTADISKVDNSKGTYAKVNGNDAVMLTLQKQSTFSTAEVAGSIRK
                     KADELSEKYGGLSIIALMDQGMYIDIVVDSVLSNVIYGGLLAILVLLLFLKDLKPTFI
                     IAVSIPISLVFAIAMMYFTGVSINIISLAGLALGVGMLVDNSIVVIENIYRLRSEGMS
                     AMDASIEGAKEIAGAIIASTLTTACVFLPIVFVKGISRQIFMDMGLTIAYSLLASLIV
                     ALTLVPAMASSMLKKASEKRNKLFDRFTEIYSGMLKWSLNYRAVVMILVVVLLGLSVF
                     LGISMGTAFIPDMEAPQMSVSIEMPKESTLQDTIAMSDRVIERIMGVEGIETIGAFQD
                     GIM"
BASE COUNT         1320 a          772 c         1114 g         1155 t
ORIGIN      
        1 ccttacttta atcaagtaaa gcggcttcaa agtggcggag agagagggat tcgaaccctc
       61 gttacgcttg tgacgtaaac acgatttcca gtcgtgcacc ttcagccatc tcggtcatct
      121 ctccaaagta gctataccgg aatttgatat ccagcatagc attatatata atacttagtg
      181 tgtcaacaga acgaatataa ttttactata tttaatagtg gttgtcaata tagcgatgtg
      241 ggaaaaataa tccccacctt ccatattgct taataattta aggcatagta aaataaaccc
      301 aagtatagca cataaagata tttcagatcc tgcggccaga cgaagagaat aaagccggga
      361 acttgggcag gtatgaaaat ttgtataggg ggctttgggt gtgagctata ataggtttga
      421 tttaatcgcc ggggatggaa ccgttataac cgttcataaa tgggggttgg aagaaggtac
      481 catagccagt ggagtggtac agatagccca tggtatggcc gagtgggcct atcggtacga
      541 ttactttgca aaggccctta accggggggg ttacatagtt tatgccaatg accatagggg
      601 ccatggatta actgccctta aaccagatga tattggatat atatccgata atgacggctt
      661 tattgatatg gtggaggata tgcgccgact taacattcta atacaagagg aatatcctac
      721 tttgcctata gtattatttg ggcatagcat gggttctttt ttaagtcaaa gatttataca
      781 gctctatggc agtgatctta gcggggtaat tctgtcaggg agcaatggaa aacaaggacc
      841 tattattaac cttggcatta cactggccta tatggagatg aagataaggg gtagaaggca
      901 taagagcagg ctgttggata atctatcctt tggcacctat aataaggcct ttgccccaaa
      961 ccggaccaaa tttgactggc tctccagaga tgaagaacag gtagatagat atatggagga
     1021 tccctattgc ggcgggatct ttacatcctc tttcttttat gattttttaa ggggattaaa
     1081 aaatataact aaaatgaaaa atttaaagga tgtccccaag gatctcccta tatatatttt
     1141 tagcgggtct atggatcctg tgggagggtt tagtaggggt gtggagagat tggtttctat
     1201 gtataaaaac cttggactaa aaagggtgtc ccggaagctg tatccaaaag gcaggcacga
     1261 gatgctaaag gagattaaca aagatgaggt aataggtgat ataatcgagt ggctaaatga
     1321 aatatgagtt tgtactggca acatgcccta ccgccccaaa agcgggtacc ccatcttaat
     1381 atgtcaaatg atataggcag gctgaatcca tgtgccctac cttgacccta gaaaattcct
     1441 atttgacatt tggtatttac ttttctataa tacataaagt aggggagtgt aggcattttc
     1501 cttgcggcac tcttgtactg attcggaatg tttggcgtaa agagataaga agtttcaaaa
     1561 aagtgtctta ctcggtatcc cggtctatgg tacctataat tctttttttg aaattatggc
     1621 cattaagttg ttatttaatc atattgcaaa caaggaatag tataacgggg gcttcagttc
     1681 agacatactc gtatagtggg tttttgggtg aagtccttgt ttttgtacaa taccgaccgg
     1741 ctggtcggtc ggacaatagc ttgtatgaat cgggaggaat tatatgttat cgaagctcag
     1801 tgtgaaaaga ccctatacgg tggtagtagc ggttataatg gtgttgatct tagggggaat
     1861 atcctttgtc aacttaaaga ccgatttact gcctaatata gatttaccct atgcccttat
     1921 tatgaccagc taccccggag ctagcccgga ggaagtagag atggtggtga caaaacccgt
     1981 cgagcaagtg gttgccacgg ttagtaatat aaaaaatgtg agctccgtat ccagggagaa
     2041 ttcatccatg gtaatattgg aatttaataa cgatgttaat atggattcgg ccaccatcga
     2101 gataaatggg atgttggatt tgattaagcc ggcatggggc gattcgatag gttcgcccat
     2161 ggttatgcgg ctaaatcccg atatgcttcc cataatgata gcgtctgtag atgtagaggg
     2221 catggacatc atcgggattt cccagatggt aaaccaagat attataccgg aattggagag
     2281 cataaccggg gttgcatccg tatcgggagt gggcctatta gaagaaaaaa tcgaagttct
     2341 catcgatggg aacaaaatag acagtcttaa caagagcatc ttagatgcgg tggattcgga
     2401 gctatccgaa gcagaggata agcttatgga tgcaaaaaac gagatagagg acggaaaagc
     2461 gaagctggaa tccgaagaaa agaaacaggg ggcaaggctg tcccagggag aaaaggcatt
     2521 gtccgatgcc aaacagcaaa ttgcccaggg tgaggtccag ctggtttctg gggaattgga
     2581 actggaaaaa gtccaggatg aattaaagac aaaattaaat gaaattaacg agaaagagaa
     2641 agagctccga gccctggcgg gcattattga tggccttaaa aagaatttac cccaaatgaa
     2701 tgagggggac ctcagagaaa cattgcttac attggtagaa aacttacctc aggagatgaa
     2761 ggagagaatc ggggctcagc tttcggaact ggaaaaaagg cttttagagg gcggcaattt
     2821 gaagcttcag gagatagaac aaattattgc cgggatctca gaagaactaa atatgggatt
     2881 agagctaatt atggcaggaa aggccgagat tgaaggcgga ctggagcaaa tctctcataa
     2941 taggcgggag ttggcggagc aaaaagcgtt actttcggca aagaaggatg aacttacctc
     3001 aaaagagcag gagataactg caggaaaact ccttttgacc atggagatgg ataaagcaaa
     3061 gtccaagctg gagagcggag aagagacctt aaatgaaaag atggaggagt ttgaatccgc
     3121 taaggaagag gcctttaaaa aagcttctct ggatggcgtt ataaccaggg agatgatctc
     3181 cggtatatta gcggcccaaa atttttctat gcctgccggt tcggtgagcg aagagggtag
     3241 tgactatttg gtcaaggtag gggataagat ccatgatata gaggaacttg aaaacctact
     3301 gctatttgat acaggggata aaggcatagg aaaaatctat cttaaggata cagcagacat
     3361 atccaaggtg gataattcca aggggaccta cgccaaggtc aacggaaacg atgcggtgat
     3421 gctgaccctc caaaagcaga gcactttttc tacagcggag gttgcaggca gtattagaaa
     3481 aaaggccgac gaactatcag aaaaatatgg gggccttagc attatagctc ttatggatca
     3541 ggggatgtat atagatatag tggtagattc cgtcctgagc aatgtgatct atgggggtct
     3601 attggctata ctggtacttc ttttattttt aaaggatcta aaacccacct ttataatcgc
     3661 tgtctccata cccataagtc tggtatttgc catcgccatg atgtacttta cgggggttag
     3721 cataaacatc atttccttgg caggtttggc tctgggggtg ggtatgctgg tggacaattc
     3781 aatagtagtg attgaaaata tctatcgcct aagaagcgag gggatgtccg cgatggatgc
     3841 ttccatagag ggggctaagg agatcgccgg tgccataata gcctccaccc tcaccacggc
     3901 atgtgtcttt ttacctatag tctttgttaa gggtatatcc agacagattt ttatggatat
     3961 gggtctaacc atagcctatt ctctgctcgc cagccttata gtggccctta ccttggttcc
     4021 tgctatggca tctagcatgc ttaagaaggc cagtgagaag cgaaataaac tcttcgacag
     4081 gttcacagaa atatacagcg gaatgctcaa atggtcgtta aattatagag ccgttgtcat
     4141 gattcttgtc gtggtattgt tgggactgag tgtattttta ggaatatcta tgggtactgc
     4201 ctttataccg gatatggagg cgcctcagat gtccgtatcc attgaaatgc caaaggaatc
     4261 caccctccaa gacaccattg ccatgtccga tagggtgata gagcggataa tgggggtaga
     4321 gggtatcgag acaataggag cctttcagga cggtataatg g
//
LOCUS       DASRNX010000100        13575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_550532,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000100 DASRNX010000000
VERSION     DASRNX010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13575
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550532"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..502
                     /gene="mraZ"
                     /locus_tag="VFD89_03950"
     CDS             71..502
                     /gene="mraZ"
                     /locus_tag="VFD89_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZJ57374.1"
                     /translation="MFIGEYQHILDPKGRVIMPSKFRDDLGENFVVTKGLDNCLFVYP
                     QGEWKELEGKLKTLPLTSKDARAFTRFFFSGATECELDKQGRILIPANLREYAGLDKD
                     LIIIGVASRVEIWGKERWEDYNSDANLDHQSIVEKMAELGI"
     gene            513..1439
                     /gene="rsmH"
                     /locus_tag="VFD89_03955"
     CDS             513..1439
                     /gene="rsmH"
                     /locus_tag="VFD89_03955"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZJ57375.1"
                     /translation="MNFHHIPVLFNETLNILGLKPGHIIVDGTLGGGGHSGGILERIV
                     PGGLLVGIDRDPNALAAARTYLHQYKDSLVTVHGNFNDLDSILDRLNISNIDGMMMDL
                     GVSSHQLDEESRGFSYHGDAPLDMRMDPTQPLSAQNVVNEYGERQLAQVILRYGEERW
                     AARIAKFIVKSRPIHTTGELTEVIKAAIPANVRRRGPHPSRRTFQAIRIEVNGELEIL
                     EGAIETAVASLKSGGRLCIITFHSLEDRIVKNTFRRLQRPCTCPPDSPICICGHKASV
                     KVITRKPIIPSEDETDENPRSRSAKLRACQKL"
     gene            1485..1958
                     /locus_tag="VFD89_03960"
     CDS             1485..1958
                     /locus_tag="VFD89_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57376.1"
                     /translation="MLVAEKKQYTGIYYPHEIEVERPISVPRPKAVPKTRSRAGKMVS
                     HIVLIIIGFAICSFTVARFAVIAQKQQEIVELGKVLERQQVIQEYMTLELASRGNLDE
                     IEEFAKNDLEMDYPHKGQILMVDLPEKTNKAEVETASLASGGKESLWSRLVSLLD"
     gene            2024..4219
                     /locus_tag="VFD89_03965"
     CDS             2024..4219
                     /locus_tag="VFD89_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZJ57377.1"
                     /translation="MAVPSVTNRKRLLAMLISFTFVFIGLIGRLGYIHLVWGKELQQK
                     AVGQWTRNLAVYPKRGMIKDRNGNILAQSGSSETIAARPSQIKDPKGVAAILAPILEL
                     DQDKLYEKLSDTKSSFVWIKRQVAREVANEVRALGIKGVDFTEEPKRYYPNRDLASHV
                     LGFTMKYAEGDDGLKGQDGIELYYDKYLKGLAGKIVMETDARGREMPNNVDRYIPPMD
                     GLNLILTIDQVVQHFTEKAVKDAMEKYAAKKVLAIVMDPNTGEVLAMVNRPNFDPNDP
                     PRELGYEGMQDYVKNFLAKDSMDPGSTFKIVTTAAALDAGAMTLESSFDCPGFRTLEG
                     NERIRCHKAGGHGHQTFAEAVENSCNPAFVDMAMALGKDKFYKYIEAFGFGQKTGIDI
                     NGEAQGILIPREAVKNLDLARIGFGQSISVTPLQLIAASAAAINGGNYMKPYLAKALT
                     ETKIDSDTGEEYEQIVKEIEPQKLRQVVSKETSQKVARILQGVVESGSGANAYIPGFR
                     VGGKTGTAQKYGKDGKIVQGRHIASFIGFAPADNPRVIVLFAVDEPQVAVDFGSVVAA
                     PYVQMILKDTLPYLGVEPAFDDETMELNRQVEVPDVMGMALGEGYAVLDERELKYLSS
                     DSVGTDTIIKDQMPKPGAKVKVNTTVLLYTQEQSPEDPGEGPEPTEGKVIVPDLKGKS
                     IREVNNVLVSLGLRLRIEGSGVAVSQDHPAESQVEPGTEITVEFAMPGE"
     gene            4315..5778
                     /locus_tag="VFD89_03970"
     CDS             4315..5778
                     /locus_tag="VFD89_03970"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZJ57378.1"
                     /translation="MKLNKLLNGIDCIKIEGNPNVDIKDMHYDSRKVTGGSLFFCIKG
                     LTVDGHDYAAQAIDRGAKVLVLERDIDADEDITKVFVKDSRAAMAYMSGNFYNNPTRG
                     ITCIGITGTNGKTTVTHLVKSILEKVGQRVGLIGTITNMVGDRKIPSKHTTPESLDLQ
                     GILRDMADEDVDSIVMEVSSHSLSLGRVEGIPYDIGVFTNLSQDHLDFHGNMEEYRDT
                     KARLFAQSKAAVINVDDEIGRWMLDRITTGVYTYGICKEADIYARELEITERGVTFNL
                     HTPGGDIGGVNLGIPGIFSIYNALAAASVCYSLGIPLDIIVQGLEAVKGVDGRFELLD
                     TGTDYSVILDYAHTPDGLENILQTADGFAKGRVITLFGCGGDRDHGKRPIMGEVAGRY
                     SDYCIITSDNPRSEDPMDIIMDIEPGVRKTSCPYTIIENRREAIEYALVHAREGDVVI
                     LTGKGHETYQILKDGTIPFDEREIVSEILDKSRTGAK"
     gene            5779..7230
                     /gene="murF"
                     /locus_tag="VFD89_03975"
     CDS             5779..7230
                     /gene="murF"
                     /locus_tag="VFD89_03975"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZJ57379.1"
                     /translation="MKSFTIRQIIDATRGKLLNDDPDFLDASVSGVSTDSRTIKKGQL
                     FIPLVGEIFDGHFFLDQVAEKGATAVLVHREDADWLPKLFPDVAAIKVPDTLKALQDL
                     ASFQRSRFDIPVVAITGSNGKTTTKDMIGLVLSKGYSVLKTQGNLNNEIGLPLTLLEM
                     DDHHQVMVVEMGMNNLGEIHRLAEIARPTIAVITNIGVSHLQNLGTRENILKAKLEVS
                     DFLGPKDWLLLNGDDEYLGCKVKGITPMVEYFGTREGADIRAQNINILGEVGVSYDLL
                     IGSDLYPMKIPVPGKHNVYNSLAAVSVGNLLDIEIPEIQNALEGFRSGDMRLNILNTE
                     SGLKIIDDVYNASPDSMTASIEILKDVGGKRKIAVLGDMLELGEYSKEGHLRVGKVVA
                     QCGIDHLITVGQDSKFIGEGALDFKMADEDMTHLECNKDVMVLLNTMLEAGDTILVKG
                     SRGLKMEEIVQYLTGWERDAKGENEQWTKLSTQ"
     gene            7206..8195
                     /gene="mraY"
                     /locus_tag="VFD89_03980"
     CDS             7206..8195
                     /gene="mraY"
                     /locus_tag="VFD89_03980"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZJ57380.1"
                     /translation="MDKIIYSVIISFIFTLALGVAIIPMLRRLRLGQSIRDDGPKSHF
                     SKAGTPTMGGIMFIIPIVIVSLALSARSLDYVIAAVLSLLGFGIIGFADDFIMVYMRR
                     SLGLRAYQKLLGQILIAVLFALYVYRSIGSSVYIPFTDIEWNLGYFYIPIIAFIMVGT
                     VNSVNLTDGLDGLASGVTLIVAMTISIIAAFAGYVMDGDGLTYIAHNYKNLSIFAAAI
                     TGACLGFLRFNVHPAKVFMGDTGSMALGGGIVAVMVLLRMPLLLPIIGGVYMAESLSV
                     ILQVISFKSRGKRIFRMSPIHHHFELGGMPETRVVAMFMIATALLCLIGLLAV"
     gene            8209..9576
                     /gene="murD"
                     /locus_tag="VFD89_03985"
     CDS             8209..9576
                     /gene="murD"
                     /locus_tag="VFD89_03985"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZJ57381.1"
                     /translation="MDFKDKRVLVVGLARSGLAAVRVLRALGANVIANDIKSREELKE
                     VIEELDPIGVQWALGVTPMDYLDKVDLVVISPGIPIDDPFVRNARDMGIEVIGEVELA
                     YRLCRAPIVAITGTNGKTTTTALAGEILTATGKRTYVVGNIGIPFVGIATEASESQIV
                     VAEISSFQLESIHAFHPKVSAILNITEDHLNRHRTMENYIGLKAGVFKNATESDWLIL
                     NADDPLSLKFAEMTRAQTLFFSRTKSLVRGAWLEDNVIVLDIGDGREKVCKVDDIFIP
                     GPHNLENAMAAVLLSRVMGVDTRTMAKVLKVFPGVDHRIELVKTLGGVTFYNDSKGTN
                     IDSTLKAIESMMSPTVLIAGGYDKGSSFDGLIEAFGDTISHLVVLGQTSDKIMQSAKD
                     KGFQNVYRVNTLEEGVKKAYSLALPGENILLSPACASWDMFKDFEERGRIFKESVRVL
                     EEGNR"
     gene            9573..10679
                     /gene="ftsW"
                     /locus_tag="VFD89_03990"
     CDS             9573..10679
                     /gene="ftsW"
                     /locus_tag="VFD89_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HZJ57382.1"
                     /translation="MKKKPMDYPIFITTIILVCFGIVMVFSSSFYMAQHNPKIQDSYY
                     FLKTQSQWAVIGLIAMVIMSNFGYWRLKKYANIILIASIGLLVALLIFGVPINNAKRW
                     FVIGGISIQPSEIMKFAMIVFLASSISKNKDRLKKPITGFGYYMVITGLIFGLIFMQP
                     NLSTAVSIVILAMIILFIGGAELWHFAALIIMGIAGVFVLLQKVDPTDYRIKRYLTFL
                     DPWADPLDDGFQVIQSLYSLGSGGIFGMGLGQSRQKYLFLPYAETDFIFSIIGEELGF
                     VGATLVIVLFLILIWRGIKIALTAPDLFGCLLASGITAVIGIQTAINIAVVTSSMPAT
                     GLPLPFISAGGSSLAFFMASIGVLLNISKYSKTT"
     gene            10915..12168
                     /gene="murA"
                     /locus_tag="VFD89_03995"
     CDS             10915..12168
                     /gene="murA"
                     /locus_tag="VFD89_03995"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZJ57383.1"
                     /translation="MAKYIITGGNKLEGSVRVTGAKNSVLPILAAALLTTREVVIKDC
                     PDLADIEKVIGILRAVGCRVKRENSTLVVDPSTITTWVIPDKYVKEIRSSIIFLGAMI
                     ARIGKAKVTYPGGCEIGQRPINLHLAGLKKLGIEVNDSYGYIECEASASVGAEIHLDY
                     PSVGATENIILAATAAHGYTIIRNAAKEPEITDLQDFINAIGGKVCGAGTSTIIVEGK
                     RPMSGAIHTIIPDRIVAGTYLTAAAITGGEVLVENVILDHVYPVVAKLRESGAIIKAS
                     GSSVIIEGRDRPLPIYHTTTLPYPGFPTDMQAPLTSLLSVSAGTSVMVETIFENRFKH
                     VPELIRMGANIRIDGKTAVIQGVEGLRGTTVTSHDLRGGAALVLAGLRAEGQTVVEDI
                     YHVERGYECLESKLGDLGARITRVE"
     gene            12217..12951
                     /locus_tag="VFD89_04000"
     CDS             12217..12951
                     /locus_tag="VFD89_04000"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HZJ57384.1"
                     /translation="MKNPRKKRHTFSVLLLLLAVTGIVYIGTDGFQIREITVVGNNRI
                     LEEDIVKRAGIPGNQNIFRLDKGLIKERLETEPYIEVLSIKRQYPYGVTITLREKEAA
                     GIIPYLNSYYVIDRDCYIMEIADSLDNFQYPLIRGIQVRNFVVGKRLIASDEYQLKVL
                     TRILESIEDLELEDQISELIVDNPEDVELILMGGMRTRVGQAIEVDKKLIWLKSQEIQ
                     EILGGVAGGILDLSAPSKPVFYSDES"
     gene            12961..>13575
                     /locus_tag="VFD89_04005"
     CDS             12961..>13575
                     /locus_tag="VFD89_04005"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HZJ57385.1"
                     /translation="MKKSTKGQYALTLISLILGVMLATQFKNVQKVGGNVSIQRTQEL
                     TEYIKKLEFDNENLNGQINEFKGIISSYENASQDETLVNELLRTKSQAGLLPMEGPGV
                     VVVVDNLNISGWDGSHQVFQNVHYDDLLRLVNELNAAGAEAIGINNERVISTTEIRNA
                     GDYIVINTNRHSAPFEIKAIGNPDNLEAALKLLGGVVDNLSQLLD"
BASE COUNT         3837 a         2768 c         3678 g         3292 t
ORIGIN      
        1 gagaatatgt aattatagtg gttgaaagtg ggggtaagtg gtgcgataat aaagaagagg
       61 ggtgaggctg gtgttcattg gtgaatatca acatattctc gatccaaagg gacgggttat
      121 tatgccgtct aaatttcgcg atgatctggg ggagaatttt gttgtgacca aaggcctgga
      181 caactgccta ttcgtatacc cccaagggga atggaaggag ctggagggca agcttaagac
      241 ccttccgttg accagcaagg atgcaagagc ttttaccagg ttctttttct caggcgccac
      301 tgaatgtgaa ttggacaagc agggtagaat cttgatacct gccaatcttc gcgagtatgc
      361 cggactggac aaggatctta taataatcgg cgttgccagc agggttgaga tctggggtaa
      421 ggaacgttgg gaagactaca acagcgatgc caatttagat catcaatcca tagtcgagaa
      481 gatggcggaa ttgggaattt aggaggatcc ggatgaattt tcatcatatt cctgtattgt
      541 ttaatgagac tctcaatata ctgggcttaa agcccggaca cattatagta gacggcacct
      601 taggcggtgg cggacattca ggcggcattt tagaaagaat cgttccgggg ggccttctcg
      661 tagggataga tagagacccc aatgctttgg cagccgctag gacatacctg catcaatata
      721 aggattccct agtaaccgtc catggaaact ttaacgatct tgattccatt ttggacaggc
      781 tcaatatatc aaatatcgat ggtatgatga tggatctcgg agtatcatct catcagctgg
      841 atgaggagag tcgaggtttt tcgtatcacg gtgatgctcc gctggacatg agaatggatc
      901 ccacacagcc attaagtgcc caaaatgttg taaatgaata tggggagaga cagctagcac
      961 aggtcatctt aaggtatggt gaggagaggt gggccgccag gatagcaaaa tttatagtca
     1021 agagccgacc tatacacacg acgggtgaat tgacagaggt gatcaaggcc gccattcccg
     1081 caaacgtaag gcgacgaggg ccccatcctt ccaggcggac ctttcaggct atcaggatag
     1141 aggtcaatgg ggaattggaa atcttagaag gcgccataga aactgcggtg gcttcattaa
     1201 aatcgggggg aaggttgtgt attattacct ttcattccct agaggacagg atagtgaaga
     1261 ataccttccg taggcttcag aggccatgta cctgtccgcc ggattcgccc atatgcatat
     1321 gcggacataa ggcctcggta aaggttataa ccagaaaacc aattattcca tcggaggacg
     1381 agacggatga aaaccctcgc tcacggagtg ccaagctgag ggcttgtcaa aaattatagc
     1441 cgggaatgag cgtcaaacat aaacattgag ggggctgaat acaattgtta gtagcagaaa
     1501 aaaagcaata cacagggatc tattaccccc atgaaataga ggtagaacgg ccgatctccg
     1561 tgccacgccc caaggcggtg cccaaaacaa gatcaagggc ggggaaaatg gtgtctcata
     1621 tagtgctaat tattataggg tttgctatat gctcatttac cgttgcaaga ttcgctgtaa
     1681 tagcccaaaa acaacaggag attgtggagc ttgggaaggt gctggagagg caacaggtaa
     1741 tccaggaata catgacattg gagctggcct cccgtgggaa cttagatgaa atagaagagt
     1801 ttgcaaaaaa tgatttggag atggactatc cccataaagg ccagatcctc atggtagact
     1861 tgcctgaaaa aaccaacaag gccgaagtgg agaccgcatc gttagcctct gggggaaagg
     1921 agagcctgtg gagcaggctg gtaagccttt tggattgaca caggcatttt tttaatgcat
     1981 ttatattata tttttaattt aaatctgtag ggtggtggta gcaatggctg tccccagtgt
     2041 tacgaacagg aaaagattgc tggccatgct gatatcattt acatttgttt ttatcggact
     2101 tataggacgt ttaggataca tacacctggt atggggcaag gaactccaac aaaaggcggt
     2161 aggacaatgg acgcgaaacc tggcggtata cccaaaaagg ggaatgatca aggatcgcaa
     2221 tgggaatatc ttagcccaaa gcggaagctc cgagaccatt gcggcccgtc ccagccagat
     2281 aaaggatcca aagggcgtag ccgccatttt ggctcctatt ttagaattgg accaggataa
     2341 gctctacgag aagttgtcgg atactaagag ttccttcgta tggataaaaa ggcaggtagc
     2401 aagggaagta gccaatgagg ttagggcgct gggcataaaa ggtgtggact ttaccgaaga
     2461 acctaagcga tattacccca accgagattt ggcttctcat gtattggggt ttaccatgaa
     2521 gtatgccgag ggcgatgacg ggttaaaggg gcaggatgga atagagctct attatgataa
     2581 atatttaaag gggctggccg gtaaaattgt tatggaaacc gatgccaggg gaagagaaat
     2641 gcccaataat gtagaccgtt atatcccgcc catggacggt ctcaacctca ttttaaccat
     2701 cgatcaggtg gttcagcatt ttacagaaaa agccgtaaag gatgccatgg aaaagtatgc
     2761 ggcaaagaag gtattggcca tcgtcatgga tcccaatacc ggcgaagtat tggccatggt
     2821 aaatagacca aattttgacc ccaatgaccc tccgagggaa ttggggtacg agggtatgca
     2881 agattacgta aaaaactttt tggcgaagga cagtatggat cccgggtcca ccttcaaaat
     2941 tgtaaccacg gctgcggcgc tggatgcagg ggcaatgacg ctggaaagca gttttgattg
     3001 tccggggttt agaaccctgg aaggaaacga gaggattaga tgtcacaagg cgggtgggca
     3061 cggacaccag acctttgccg aggccgtgga aaactcctgc aatccggcat tcgtggatat
     3121 ggctatggct cttggtaagg ataaattcta taagtatatt gaggccttcg gttttgggca
     3181 aaaaacgggt atagacataa acggagaggc ccaagggatt ctaatcccca gagaggccgt
     3241 gaagaattta gacctggcca gaatcggatt cggtcaatcc atatcggtaa ctccgctcca
     3301 gcttattgca gccagtgcgg cggcaatcaa cggcgggaac tatatgaagc cctatcttgc
     3361 aaaggcgctg acggagacca agatagattc tgacactggc gaggagtatg agcagatagt
     3421 caaggagata gagccgcaga aattgcgcca ggtggtatca aaggagacct cccagaaggt
     3481 cgctaggata ctccagggag ttgtagagag cggaagtggg gctaacgcct atataccggg
     3541 attcagggtg ggaggaaaga ctgggaccgc ccaaaaatat gggaaggacg gcaaaatcgt
     3601 tcagggaagg catatagcct cctttatcgg gtttgcaccc gccgataacc ctagggttat
     3661 agtgttgttc gccgttgacg agccccaggt agccgtagac ttcggtagcg tagtagctgc
     3721 gccctacgta cagatgattc tcaaggatac attgccgtat ctcggggttg agcccgcctt
     3781 cgacgacgag acaatggagc tcaacagaca ggtggaggtc ccggatgtca tgggtatggc
     3841 ccttggtgag ggttatgccg ttttagatga aagggagctg aaataccttt ccagcgattc
     3901 ggtgggaacg gataccataa taaaggatca gatgccaaaa cccggcgcca aggtcaaggt
     3961 aaatacgacg gttcttctgt acacccagga gcaatcgccg gaggatcccg gtgaaggtcc
     4021 ggagccgacg gaagggaagg tcattgttcc cgaccttaag ggcaaatcca tcagggaagt
     4081 aaacaatgtt ttagtgtctc tgggcctcag gctaaggatt gagggctccg gggtagcggt
     4141 gagccaggac caccccgcag aaagccaggt tgagccgggt actgaaatca cggttgaatt
     4201 tgctatgccg ggggagtaat atatagtggg ttttaacttt acgccttaaa ggctataata
     4261 gatgtaggtt aatctgacgc gacgtgccca agaaataata ggacggtatc ttgaatgaaa
     4321 ctaaataaat tgctaaatgg tatagactgt ataaaaatcg aaggtaatcc aaatgtagat
     4381 attaaagata tgcactatga ttccaggaag gtgaccggcg gatccctgtt tttctgtatt
     4441 aaggggttga cggtggacgg acacgactat gctgcccagg ccatagacag gggggcaaag
     4501 gtgttggtac ttgaaaggga catagatgca gatgaggata taaccaaggt gtttgtcaaa
     4561 gattccaggg cagccatggc atatatgtcc gggaatttct ataacaatcc cacccggggt
     4621 attacctgta taggtatcac gggtaccaac ggaaaaacta ctgttaccca ccttgtaaaa
     4681 tctatcctag agaaggtggg gcagagggtt ggccttatag ggaccataac caatatggtg
     4741 ggagacagaa agatcccatc aaaacatacc accccagagt ccttggatct tcaggggatt
     4801 ttgagggata tggccgacga agacgtagac agcatagtta tggaggtctc gtcccattcc
     4861 ctaagcctag ggagggtcga gggaatccca tacgatatcg gggtatttac caatctatcc
     4921 caggaccatt tggattttca cgggaatatg gaagaatata gggataccaa ggccaggctt
     4981 tttgcccaga gcaaggcggc tgtcataaat gtggacgacg agataggcag gtggatgttg
     5041 gataggatta ccaccggggt ctacacctat ggcatctgca aagaggcgga tatatatgca
     5101 agggaactgg aaatcaccga aaggggtgtt acctttaacc tgcatacccc tggcggcgac
     5161 ataggcggcg taaacctggg tatacccggt atcttcagca tatacaacgc tctggctgcg
     5221 gccagcgtgt gctatagttt gggtataccc ttggatataa tcgtccaagg gctggaggcg
     5281 gtcaaaggcg tggatgggcg ctttgagctt ttagataccg gaaccgatta ttcggtgata
     5341 ctggactatg cccatacccc ggatggcctg gaaaatattc tacagaccgc cgatggattc
     5401 gcaaagggca gggttataac cctatttggc tgcgggggag acagggatca tggcaaaagg
     5461 cccatcatgg gggaggttgc cggaagatat tcggactatt gtatcataac ttccgacaat
     5521 ccccgcagcg aggatcccat ggatataatt atggatatag agccgggagt aagaaaaaca
     5581 agttgtccct acaccataat agaaaatcgc agggaggcca tagaatatgc ccttgttcat
     5641 gccagagagg gtgatgtggt tatacttacc ggcaagggac atgaaacata tcaaatccta
     5701 aaggatggga ccataccctt cgatgaaagg gagatagtct cagaaattct agataagagc
     5761 aggacaggag caaaatagat gaaatccttt actataagac aaataataga cgcaacccgg
     5821 ggcaaactcc taaacgatga tccagatttt ctggacgctt cagtatccgg ggtgagtacc
     5881 gatagcagga caattaagaa aggtcagctc ttcattccct tggtggggga aatttttgac
     5941 ggacactttt ttctggacca ggtagcggaa aaaggggcaa cggctgtgct tgtacatagg
     6001 gaggatgcgg attggctgcc gaagctcttt cccgatgtgg ccgccataaa ggtgcccgac
     6061 accctaaaag ctctccagga tttggcatcc tttcagcgga gccggttcga tatcccggtg
     6121 gtagccataa ccggcagcaa cggaaaaacg acaaccaagg atatgatcgg gctggtgcta
     6181 tccaagggat atagtgtcct aaagacccag ggaaacttaa acaacgaaat aggtctgccc
     6241 ctgacgcttt tggaaatgga tgaccatcat caggtcatgg tagtggagat gggaatgaat
     6301 aacctggggg aaattcacag attagcggag attgcaagac ctacaatcgc cgttatcacc
     6361 aatataggtg tgtcccacct ccagaatttg gggactaggg aaaatatcct aaaggcaaag
     6421 ctggaagttt ccgacttttt gggtccgaag gattggttgc tgctaaacgg ggatgacgag
     6481 tacctaggct gtaaagtgaa ggggatcacc cccatggtag aatactttgg tacccgagag
     6541 ggtgcagaca ttagagccca aaacataaac attttagggg aggtgggggt atcctacgac
     6601 ctcttaatag gcagcgatct atatcccatg aaaatcccgg tgcccggcaa acacaatgtc
     6661 tataatagtc tagcggccgt ttcggtgggg aaccttttgg atattgaaat accagaaatt
     6721 caaaatgctc tagaaggctt tcgctccggg gatatgcggc tcaatattct aaacaccgaa
     6781 tccggattaa agattattga cgatgtctac aacgcaagcc cggattctat gacagcctcc
     6841 atagagattt taaaggatgt cgggggcaag cgaaagattg ccgtcttggg cgacatgctg
     6901 gagcttggcg aatactctaa agaaggtcat ctgagggtag gcaaggttgt agcccaatgc
     6961 ggtatcgatc atttgattac cgtaggccag gacagcaagt ttataggcga aggggctttg
     7021 gatttcaaga tggcggatga ggatatgacc cacttagagt gtaataaaga tgtaatggta
     7081 ttgcttaaca caatgctcga ggcaggcgat acaatattag tgaagggatc caggggccta
     7141 aagatggagg agatagtcca gtatctgacg ggttgggaaa gagatgccaa gggggagaat
     7201 gagcaatgga caaaattatc tactcagtaa taatatcctt tatatttacc ttagccttgg
     7261 gcgtcgccat tattcccatg ctaagaaggt taagactggg acagagtatc cgcgacgacg
     7321 gaccaaaatc ccatttttcc aaggccggga cacctaccat gggcggaatt atgtttataa
     7381 ttcccatagt tatagtctcc ttggctctat ccgccaggag cctcgattat gtgatagccg
     7441 ccgtactgtc ccttctgggt ttcggtatca ttggatttgc agacgatttt attatggtat
     7501 atatgaggcg ctccctgggg ctcagggctt accaaaagct tttagggcag atcttaatag
     7561 ccgttctgtt tgccctatac gtgtatcgga gcattggcag cagcgtctat attcccttta
     7621 ccgatatcga atggaatctg ggttactttt atatccccat catagccttt ataatggtgg
     7681 gcactgtcaa cagcgtcaac ctgaccgatg gactcgacgg tttggcctcg ggggttaccc
     7741 taatcgttgc catgaccata agcattatag ccgcctttgc cggctatgtc atggacgggg
     7801 acggtcttac ctatattgca cataattaca agaacctgtc catattcgca gctgctataa
     7861 ccggcgcttg tctgggattc ttaaggttta atgtccaccc cgccaaggtc tttatgggcg
     7921 ataccgggtc aatggctcta gggggcggta tagttgcggt gatggtcctc cttaggatgc
     7981 ccctgttgct tcctataatc ggcggagtct atatggcaga gtctttatcg gttatacttc
     8041 aggttatatc ctttaagagc aggggtaaac gaatatttcg gatgagtccc attcatcatc
     8101 attttgaact gggaggcatg cctgagacca gagtggtggc gatgtttatg attgcaacgg
     8161 ctcttctctg tcttataggc ttgttagcgg tatagaggag agtaaactat ggattttaaa
     8221 gataaaagag tattggtagt gggacttgcc cgcagcgggt tagcagcggt aagggtttta
     8281 agggcccttg gggccaatgt aatagccaac gatataaaaa gccgggaaga gctcaaagag
     8341 gttatagaag agctggaccc tataggagtc cagtgggctc tgggagtcac ccctatggat
     8401 tacctagaca aggtggatct agtcgtcata agccccggca tacccataga cgaccccttt
     8461 gtgcgaaatg cacgggatat gggcatagag gttataggcg aagtggagct ggcctatcgc
     8521 ctatgcaggg cacccattgt ggccatcaca gggaccaatg gaaagaccac caccaccgct
     8581 ttggctggtg aaatcctgac ggcgacgggc aagagaacat atgtggtagg caacatagga
     8641 atcccctttg tcggaatagc aactgaggct tctgaatcgc aaatcgtggt ggcagagatc
     8701 agcagctttc agctggaatc aatccacgcc tttcatccta aagtatctgc catattgaat
     8761 atcaccgagg atcatctcaa tagacatagg actatggaaa actatatagg gcttaaagcc
     8821 ggtgttttta agaatgctac cgaatcggat tggcttattc taaatgctga cgatccccta
     8881 agtcttaagt ttgccgagat gactagggcc cagaccctgt tctttagcag aaccaaaagc
     8941 ctagttaggg gagcatggtt ggaggataat gtgattgtcc tggatatagg cgacggcagg
     9001 gaaaaggtgt gtaaggtcga cgatatcttt atacccgggc cccataacct ggaaaacgct
     9061 atggctgcgg ttttattgtc cagggttatg ggcgtcgaca cccggaccat ggcaaaggtt
     9121 ttaaaggtat ttccgggggt ggatcacaga atagaactgg ttaaaaccct tggcggcgtt
     9181 accttctata atgattctaa gggtaccaat atagattcca cattaaaggc catagagtcc
     9241 atgatgtcgc ccacggtcct aatagccggg ggctacgaca agggtagtag ctttgacggt
     9301 ctgatagaag ccttcgggga taccataagc cacttggtgg tacttggtca aacatcggat
     9361 aaaataatgc aatctgccaa ggataagggc ttccaaaacg tttacagggt aaatactttg
     9421 gaagaggggg taaaaaaggc ctacagcctt gcgctacccg gcgaaaatat cctactctcc
     9481 cccgcatgtg ccagttggga tatgtttaag gattttgagg aaaggggaag gatatttaaa
     9541 gagtctgtaa gggttctcga ggaggggaat agatgaaaaa gaagcccatg gactacccca
     9601 tctttattac caccatcata ttggtatgct ttggaatagt tatggttttt agctccagct
     9661 tctacatggc ccagcacaac ccaaagatcc aagacagcta ttattttctg aaaacccaat
     9721 cccaatgggc agtaataggc ctgattgcta tggttatcat gtccaatttt ggctattgga
     9781 gattaaaaaa atatgccaac ataatactca ttgcaagtat cggattgttg gtagccctcc
     9841 taatatttgg ggtacctatt aacaacgcca agcgttggtt tgttataggt ggcatcagta
     9901 tacagccctc ggaaattatg aagtttgcta tgatagtgtt tttagcctcc agtatttcca
     9961 agaacaagga tcgcctaaaa aaacctatta caggttttgg ttattatatg gtcattacgg
    10021 ggctgatatt cgggcttata tttatgcaac ccaacctgag caccgccgta agcatagtta
    10081 ttctggctat gatcattctg tttataggtg gggccgaact atggcatttt gcagcgttga
    10141 tcattatggg tatagcgggc gtatttgtgc tccttcagaa ggtggaccca accgactatc
    10201 gtattaagcg gtatctcacc tttttagatc cctgggcaga ccctctagat gacggatttc
    10261 aggtcattca atccctgtat tccttaggat ccgggggtat attcgggatg ggcttggggc
    10321 agagtagaca aaaatacctt tttcttccct atgccgagac ggactttata ttctctatca
    10381 taggcgaaga gttgggcttt gtgggggcta cattggtcat cgtattattt ttgattctga
    10441 tctggagggg gatcaaaatc gcgctgaccg cgccggacct gtttggatgt cttttagcct
    10501 ctgggataac tgcggttata ggaatacaga ctgccatcaa catagccgtt gttacctcct
    10561 caatgccggc aacgggcttg cccctgccct ttatcagtgc gggaggatcc tccttggcgt
    10621 ttttcatggc cagcatcggc gtgctcctca atatatccaa atacagtaag acgacttagg
    10681 atttggggaa taaattaatt cattgtgtga gtccattaac ggtgtatacc cggctagaac
    10741 aacactttaa aataggtatc attaaagaat taggcagtcg ggctaaaaaa cttaggttat
    10801 cgacattata gcctaaagca gcgaatgact tatatttggg atttccacag tcacacctct
    10861 tttcaggatg catatcatgg tagggcagga tagcatataa gagaggtgta tgccgtggca
    10921 aagtatataa ttaccggcgg aaataaactg gaaggctcag tcagggtgac aggggcaaaa
    10981 aattctgttt tacccattct ggcagctgcc cttttaacta ccagggaagt ggttataaag
    11041 gattgtccgg atttggccga tatagagaaa gtgattggaa ttttaagagc cgtcggatgc
    11101 agggtgaaaa gggaaaatag taccttggtg gtagatccct ccaccattac cacttgggta
    11161 atcccggaca agtatgtcaa ggagataagg tcctccatta tatttttagg agcaatgata
    11221 gcgaggattg gaaaggccaa ggtgacgtat ccgggagggt gcgaaatcgg acagagacca
    11281 ataaatctac atttggcggg cctgaagaag ctggggatag aagtaaacga ttcctatggt
    11341 tatattgaat gtgaggcctc ggcctctgtg ggagccgaaa ttcatttgga ttatcccagc
    11401 gttggtgcga cagagaatat tattttggct gctaccgcgg cccatggtta taccataata
    11461 cgcaatgccg ccaaggaacc ggagataacg gatctgcagg actttataaa cgccataggc
    11521 ggaaaggtat gcggtgcggg aaccagtacc ataatagtag agggcaaacg tcccatgagc
    11581 ggagcgatac ataccatcat tccagatcgt attgtggccg ggacctatct gacggccgca
    11641 gctataaccg gcggtgaggt gcttgtagaa aatgtaatat tggaccatgt atatcccgtt
    11701 gtcgcaaagc tgagggagtc cggtgctatt atcaaggcct ccggatcgtc tgttatcata
    11761 gagggaaggg atagaccgct tcctatatat catactacga ccctgccgta cccgggtttc
    11821 ccaacggata tgcaggctcc acttaccagc cttctgtcgg tatccgcagg caccagcgtt
    11881 atggtagaga cgatatttga gaataggttc aagcatgtcc cggaattgat acgcatgggc
    11941 gctaatatca ggatagacgg aaagacggcg gttatccagg gagtagaggg ccttaggggt
    12001 actacagtta cctcccacga tctaagaggc ggcgcagcgt tggtcctggc cggattgagg
    12061 gcagaggggc agaccgtcgt ggaggacata taccatgtcg agcggggata tgagtgcctg
    12121 gaatctaaac tgggggatct gggtgcaagg attactaggg tagaataacg gcgccgacaa
    12181 gagaaaaatt ctgagctcta aagataaggt ggttgcatga aaaacccgag aaaaaaaaga
    12241 cacacgttct cagttttgtt attgctattg gcagtaacgg ggatcgtgta tataggcacc
    12301 gacggttttc aaataaggga gattactgtt gtgggcaaca atagaatcct agaagaggat
    12361 atagttaaaa gggccgggat ccccggcaat caaaatatct ttaggcttga taaagggctt
    12421 ataaaggaaa gactggaaac agagccctat atagaggttt tatccataaa gcggcaatac
    12481 ccctacgggg ttactattac cttgagggaa aaggaagcgg cggggattat tccatactta
    12541 aactcgtatt atgtcattga cagggattgc tacataatgg agatagccga ctccttggat
    12601 aatttccaat atcccctgat ccgtggtata caggtaagaa attttgtggt gggaaaaagg
    12661 cttattgcct cggatgaata ccaattaaag gtcctcacaa gaatccttga atccatcgag
    12721 gatttggaat tggaggatca aatttctgaa ctgattgtgg ataaccccga ggacgttgag
    12781 cttatactga tggggggaat gcggaccagg gttggacaag caatagaggt ggataaaaag
    12841 ctgatctggc ttaaaagcca agaaatacag gagatcctag gcggtgtagc ggggggaatc
    12901 ctggacctga gcgccccgtc aaaaccggtt ttttattctg atgaaagctg aggtgaattc
    12961 atgaaaaaat caactaaggg acaatatgcc ctgaccctaa tcagcctgat tttaggagtt
    13021 atgctggcaa cccagtttaa aaatgtacaa aaggtgggcg gcaatgtttc aattcaaagg
    13081 acccaggagc tgacggagta tattaagaag ctggaattcg acaacgaaaa tctcaacggc
    13141 cagattaacg aatttaaggg aattataagc agctacgaaa atgcttccca ggatgagact
    13201 ttggttaacg aactccttag aaccaagagt caggccggcc tgttgcccat ggagggtccg
    13261 ggggtggttg tagttgtaga caatctcaat ataagcggtt gggacggaag ccaccaggtg
    13321 tttcagaatg ttcattacga tgaccttctg agattggtaa acgaattaaa tgcagctggc
    13381 gccgaggcca tcggtatcaa taacgaaagg gtaatttcca caaccgagat ccgcaatgcc
    13441 ggggattata ttgtcatcaa taccaacagg cattcggcgc cctttgagat aaaagctatc
    13501 ggtaacccgg ataacttgga ggcagcatta aagcttttgg gcggtgtagt ggacaaccta
    13561 agccaacttc tggac
//
LOCUS       DASRNX010000101         6661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_179094,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000101 DASRNX010000000
VERSION     DASRNX010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179094"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFD89_04010"
     CDS             complement(<1..724)
                     /locus_tag="VFD89_04010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZJ57386.1"
                     /translation="MYTNLLNDFIENTKNIMINNILVLKDGEEIAAYNWEPEIRQNQY
                     SVSKSFTSSAMGIAVGEGLLTLDDPVIDFLPEKVPDNPPDRLRRLKVRHLLTMGVGHD
                     ESHLMAEQRYTTEETDWVRLSLAIPMDFEPGARFVYSNVGPYLAGMIIRRLTGSDLRD
                     YLMPRLFEPLGIWLPTWETDPLGNSFGSGGLMLKVSEIAKLGQLYLQGGQWEGKQLIP
                     KQWVEEVSKTLILTGADSPHNAG"
     gene            complement(875..1681)
                     /locus_tag="VFD89_04015"
     CDS             complement(875..1681)
                     /locus_tag="VFD89_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZJ57387.1"
                     /translation="MLWEHLVPKDFKKAIEETGLCIVPIGSLERHGEHLPFGCDMIIA
                     HTIAARAAEIEPAVVFPPYFLMQVHEAACFTGTVNFPQAFAIEAFGHLLDSIAQNGFK
                     KILIVNGHGGNSHMLDYFSMSRLDEPTDYIFYQTSVSGALTEDERKTIEDLWDTNLGG
                     HACEMETSLFMACCPGKTKLEYAPKEEVKPLGRFKHLHENGVNNALWWYADYPENVVG
                     DAKYASEEKGRVAADIYARSLARAIKAVKEDTTAPALQEEFSKKVKEKGI"
     gene            2038..2493
                     /locus_tag="VFD89_04020"
     CDS             2038..2493
                     /locus_tag="VFD89_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZJ57388.1"
                     /translation="MKEKTISFIEIWDLLIDVQSLMNVLSRKVQAQYDLAPQELRVML
                     ELDRAGRMNISELSKAINRDFGNVSRTCAALVNKGLLIRQRSERDQRVTLMTLTGKGK
                     HKLEVFYQYNLTVMQSKVFMENEDKCRAMMTGMMLFLSFVAERMEELDD"
     gene            2486..4333
                     /gene="ftsH"
                     /locus_tag="VFD89_04025"
     CDS             2486..4333
                     /gene="ftsH"
                     /locus_tag="VFD89_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZJ57389.1"
                     /translation="MIKQSNGNRKPKTPRPILYYYGIALLALLLLNWLVLPYLTKPQV
                     VEVGYSFFLDEVESGKVTTVEMDTNQILFSIVDNHEKESYYKTGYVNDPELVSLLRGH
                     GVEFGAVIPKQPNYFLSLLLSYGIPLLIIWWIGRKMSKTLGDRMGAGGLALGGNAGKV
                     YVPTGDKKTFHDVAGQEEAKEALTEVVDYLSHPERYSAIGAKCPKGVLLIGPPGTGKT
                     LMAKAVAGEADVPFFSIAGSEFVEMFVGRGAARVRDLFEQAKKKAPCIVFIDEIDTIG
                     KRRDGGIGTNDEREQTLNQLLTEMDGFEGDSGVIILAATNRPETLDPALLRPGRFDRQ
                     VPVELPDLQGRIAIFKVHAKNYKVVDSIDFDLLGRATVGASGAEIANIVNEASLRAVR
                     MGHHKVLQEDLEESIEVVIAGHQKKNAILSPEEKKIVSYHEVGHALVAANQSNTAPIH
                     KITIVPRTSGALGYTMQVEENEKSLMTRTEAFNKMVTYCGGRAAEEIIFGEITTGASN
                     DIEQVTRIARAMITRFGMSDEFGMMALETNNNMYLGGDSTLNCSPKTGELVDKEVKEL
                     IRSAYQKARDILIENKAKLHELAEFLYREETITGEQFMGIMDRENKYSA"
     gene            4838..6628
                     /locus_tag="VFD89_04030"
     CDS             4838..6628
                     /locus_tag="VFD89_04030"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HZJ57390.1"
                     /translation="MTTNQKLLDWVKEMAEMCQPDKIYWCDGSEEENDRLLKEMVDSG
                     AAVELDPEKKPGCYLFRSDPSDVARVEDRTYISSKNKEDAGPTNNWVDPTELKNTMKE
                     LYSGCMKGRTMYVIPFSMGPVGSPASKIGVELTDSPYVVTNMRIMTRMGKEVLDVLGD
                     GEFVPCLHSVGAPLAEGEKDVPWPCAPMEQKYISQFPEDRTIWSYGSGYGGNALLGKK
                     CFALRIASVQARDEGWLAEHMLILKLTSPEGEVKYVTGAFPSACGKTNLAMLIPTIPG
                     WKVETIGDDIAWMRFGEDGRLYAINPEAGFFGVAPGTSMDSNPNAMHSIEKNTIYTNV
                     ALTDDGDVWWEGIGSDAPDHLIDWHGNDWTPGSDRPAAHPNARFTAPASQCPVIAPEW
                     EDPAGVPISAILIGGRRPSTIPLVHESFDWEHGVFMGSIMGSEITAAAISDNIGQVRR
                     DPFAMLPFCGYHMGDYLNHWLTIGKNADTDKLPKLFYVNWFRRDDDGNWLWPGFGENS
                     RVLKWVFERTSGQGEAVKTPIGYMPTPDAIDTEGLDVSEADMEELLDVNKEQWLREVE
                     SIKEYYKMYEDKLPQELAQQLEALEERLRS"
BASE COUNT         1817 a         1503 c         1673 g         1668 t
ORIGIN      
        1 atccggcatt atggggggaa tctgccccgg ttaggatcag ggttttggac acctcttcta
       61 cccactgttt ggggatcaat tgctttccct cccactggcc gccttgaagg tagagctgcc
      121 ctagcttggc tatttcagat actttaagca tcagcccccc tgacccaaag ctgtttccta
      181 aaggatcggt ttcccaggtg ggaagccata tcccgagggg ctcaaagagc ctgggcatca
      241 gataatcccg taggtcagaa cccgttaatc ttctaattat catccccgcc aaataaggtc
      301 ccacattgct atagacgaag cgggctccgg gttcaaagtc catgggaata gccagactga
      361 gccttaccca atctgtctct tccgtggtgt atctctgttc tgccatgagg tgggattcat
      421 cgtgccccac acccatagtg agaagatgcc taacctttag acgccgcaac ctatccggcg
      481 gattatccgg taccttctcc ggtaaaaagt ctataacggg gtcatccaag gttaataatc
      541 cctctcccac ggcaatcccc atagccgatg aagtaaaact tttgctcacg gagtattgat
      601 tttgtcgtat ctcgggttcc caattatatg cggcaatctc ttccccgtct ttgagaacca
      661 ggatattatt gatcatgatg ttcttagtat tttctatgaa gtcattaagt aagttggtat
      721 acatattaca ccctcctaaa ccggcacgcc tcgaattcct atggtaataa aagcatcgcc
      781 taaatgtttt taaaaacggt caatggggtt cttgcaaaat cggaattatc ctcggcacga
      841 cataacctta aggacaatat gtccttcggg aaccctaaat tcccttctcc ttgacctttt
      901 tggaaaattc ctcttgaagg gcgggcgctg ttgtatcctc cttaacagcc tttatggccc
      961 tggccaagga tcttgcatag atatctgccg caaccctacc cttttcttcg cttgcgtatt
     1021 ttgcgtcccc tacaacgttt tccggataat ccgcatacca ccacagggca ttattcaccc
     1081 cattttcgtg cagatgttta aagcgcccca ggggtttaac ctcttctttt ggggcatatt
     1141 ccaatttcgt cttgccggga caacaggcca taaaaaggga tgtctccatt tcacatgcgt
     1201 ggccacccag attcgtatcc catagatcct ctatggtttt tctctcatct tctgtaaggg
     1261 cgccgctgac acttgtctgg taaaatatgt agtccgtggg ttcgtctaaa cggctcatgg
     1321 aaaagtagtc cagcatatgg gaattgcccc catgcccgtt gacgatcaat atctttttaa
     1381 acccgttctg ggcaatggag tccaacaaat ggccaaaagc ctctatggca aaggcctggg
     1441 gaaaattcac ggtacccgta aaacacgcag cctcatggac ttgcatcaaa aagtaaggcg
     1501 gaaagactac ggcgggttcg atctctgcag ctctggcggc tatggtatgg gctatgatca
     1561 tatcacatcc aaagggtaaa tgttcgccat gcctttccag tgaacctata ggtactatac
     1621 aaagtcctgt ttcctctatc gcttttttaa aatccttcgg tacaagatgt tcccaaagca
     1681 tttcgtcccc tccatcttca tttttttatt tgttattctg acatgctgtc attcccacga
     1741 cggtgctgag cattcaataa ccgttacaga catatgctta accgacacag cacgttaatc
     1801 aatccgttat gccggccgga tatgtatagt ttattacctt tttctaataa aatcaatagc
     1861 cccatagccg tatacccccg ccctataagg gcctccctga aagttatttt ctgtaacagg
     1921 ggttccatct tagggaaaat ggcctgatcc tgtcatatat ccgttgcatg gctccctgaa
     1981 aggtggtata atatatgtat gtaacataca agtgtataag gggagtgagg gtgtgaggtg
     2041 aaagaaaaaa ctatatcctt tatagaaata tgggatcttt tgatagacgt tcaatcctta
     2101 atgaatgtat tatcacgaaa ggtgcaggcg cagtacgacc tggcgcccca agagttgcgg
     2161 gtgatgttag agctggatcg cgcagggaga atgaatatca gcgaattgtc caaggcaata
     2221 aatcgagatt ttggaaatgt cagccgcacc tgtgccgctt tggttaacaa gggactgtta
     2281 atccgtcaga ggtccgagag ggatcaacgg gtcaccctga tgacattaac gggaaagggc
     2341 aagcacaaat tagaggtatt ctaccagtac aacttgactg tgatgcaaag taaggttttt
     2401 atggaaaacg aggacaaatg cagggctatg atgacgggaa tgatgctgtt cctttccttt
     2461 gtagccgaga gaatggagga actagatgat taagcaaagc aatgggaata gaaaaccaaa
     2521 gactccaagg cccatcctgt actattatgg catagccttg ttggcacttt tattgctgaa
     2581 ctggcttgtg ttgccatatc ttacgaagcc tcaggttgta gaggttggct attccttctt
     2641 tttagatgag gttgaaagcg gcaaggttac tactgttgag atggatacta atcagatcct
     2701 gttttccata gtagataacc atgagaagga gtcatattac aaaaccggat atgtcaatga
     2761 cccggagctg gtgtcactct tgcggggaca cggggttgaa tttggggctg taatacccaa
     2821 acaaccaaat tactttttga gcctgctctt atcctatggg attccgctcc tcatcatctg
     2881 gtggatcggc aggaagatga gtaagacctt aggggatagg atgggtgccg ggggcctggc
     2941 ccttggcgga aatgccggaa aagtctatgt accgacgggc gataaaaaaa cattccatga
     3001 tgtagcggga caggaagaag cgaaggaggc ccttacggaa gtggtagatt atctgtccca
     3061 ccccgaaaga tattctgcca taggagcaaa atgtcccaag ggtgtactcc taatcggtcc
     3121 accggggacc ggtaagaccc taatggccaa agcggtagcc ggggaagcgg atgtaccttt
     3181 cttttccata gcggggtcag aattcgtgga gatgtttgtg ggtcggggtg ctgcgagggt
     3241 cagagacctc tttgaacagg ctaagaagaa ggcaccgtgt atcgtattta tcgatgagat
     3301 tgatactata ggtaaaagac gtgacggcgg cataggcacc aacgatgaga gggagcaaac
     3361 tctaaaccaa ttgcttactg aaatggacgg atttgaagga gattcggggg taatcatact
     3421 ggcagctacc aaccgtcccg agacattgga tcccgcgcta ttacggccgg gtcgatttga
     3481 cagacaggtc cccgtggaac tgccggattt acagggccga atcgccatct ttaaggtgca
     3541 cgccaaaaac tataaggtgg tggacagtat cgattttgac ctattaggcc gggcaaccgt
     3601 aggtgcctcc ggggcagaga tcgccaacat cgtcaacgaa gcctccttaa gagccgtgag
     3661 aatgggtcac cataaggtgt tacaggagga tctggaggag tccatcgaag tggtcatagc
     3721 cggccatcag aagaaaaacg ctatcctctc tccggaagaa aaaaagatag tctcctatca
     3781 tgaagtcggc catgcgctgg tagcagccaa tcaaagcaac accgccccca tccataagat
     3841 aaccatcgtc cccagaactt ccggggcgct gggttatact atgcaggtgg aggaaaatga
     3901 aaaatccctt atgaccagaa cagaagcctt caataagatg gtcacctatt gtggcgggag
     3961 ggccgccgag gagataatat tcggggagat tacaaccggg gcctccaatg atatcgaaca
     4021 ggtaaccagg atagccaggg ccatgattac acgctttggg atgagcgacg aatttggtat
     4081 gatggcttta gagaccaaca acaatatgta tctgggcggg gattctaccc taaactgttc
     4141 acccaaaacc ggtgaattgg tggacaagga agtgaaggag ttgatccgtt ccgcctatca
     4201 aaaagccagg gatatcctta tagaaaacaa ggctaaactc cacgagctgg cggaattcct
     4261 atacagggag gaaaccataa ccggggagca gtttatggga ataatggata gagagaataa
     4321 atatagcgct tgaacctctt atgactaaag tcatgagatt ttgttgtcac cagctgaggg
     4381 tgggggggtg gcctagctgg tcccccctta tttgcgtacc tggcatgggt ggtaacttgg
     4441 aagtgagagt ccgctgtggg ttagtagtgg aaaccactag ccaaaggcaa ggatgtccat
     4501 cgtgaggtgg aacctgaagg aagccctatg tggagtccct aagccggctt ccccggtcat
     4561 agacagcgca aaattctaca gccccggata gatgttgggg gtacatatag tacggcttaa
     4621 tttgtaagca aggggctata aatagaaaaa ggtccaaaat aataaaaata tttatggcaa
     4681 attatatatt tttatgataa gatatcatgg ggtacaatag ttatattaga ataccaaaat
     4741 aattttgata tatttaacat aactatcaat gcgttcatgg caacaaacaa tagtgtacaa
     4801 tattaatcaa taaaggtgtg attagatgaa tgacaagatg accactaacc aaaagttgtt
     4861 agattgggtt aaagagatgg ccgaaatgtg ccagcctgac aagatctatt ggtgtgacgg
     4921 ctcggaagaa gaaaacgatc gtctacttaa ggaaatggtg gacagtggtg cggcggtgga
     4981 gctggaccct gagaaaaaac cgggttgcta tcttttccgg agcgacccct ctgatgtagc
     5041 tcgtgttgag gatagaacct atatatcctc caagaataaa gaagatgcag ggccgaccaa
     5101 taactgggtt gaccccactg aattaaagaa taccatgaag gaattatatt ctggttgtat
     5161 gaagggtaga acgatgtatg tcattccctt ctccatgggt cctgtaggtt cacccgcttc
     5221 aaagatcggg gtagaactga cggatagtcc ctatgttgtt accaatatgc ggattatgac
     5281 ccgtatgggc aaagaagttt tggatgttct tggggacggc gaatttgtac cctgccttca
     5341 ttcagtaggt gccccattag ctgaaggcga aaaagacgtc ccttggcctt gtgcccctat
     5401 ggaacaaaag tatataagcc aattcccgga agatcggaca atttggtcct acggctccgg
     5461 ctatggcgga aacgcactgc ttggcaaaaa atgttttgcc ctcagaatcg cttccgtcca
     5521 agccagggat gagggttggt tggctgagca tatgcttata ctcaagctta caagccccga
     5581 aggcgaagtt aaatatgtta ccggagcctt ccccagcgca tgcggaaaga ccaatctggc
     5641 tatgcttata cctacaattc cgggttggaa ggttgaaaca attggcgacg atatcgcctg
     5701 gatgagattc ggagaggacg gccgtctgta tgccatcaat cctgaagccg ggttcttcgg
     5761 cgttgccccc ggtacctcca tggattccaa tccaaatgct atgcacagca tagaaaagaa
     5821 tacgatatac accaatgtcg ccctaaccga cgacggggat gtctggtggg agggcatcgg
     5881 ttccgatgca ccggaccatc tcatagattg gcatggtaat gattggactc cgggtagcga
     5941 taggcctgca gcccatccta acgcgcgctt caccgctccc gcaagccaat gtccagtaat
     6001 cgccccagaa tgggaagacc ctgcgggagt gcctatctcg gcaatcctaa tcggcggacg
     6061 ccgaccaagc acgattcccc tagtacatga gagctttgat tgggagcatg gcgttttcat
     6121 gggttccatc atgggctctg agattacagc cgccgctata tccgataata tcggtcaggt
     6181 tagacgcgat cccttcgcta tgttgccttt ctgtggctat cacatgggag attacctaaa
     6241 ccattggctg accatcggta agaacgctga taccgacaag ctgcccaagt tattctatgt
     6301 gaactggttc cgcagagacg atgatgggaa ttggttatgg ccaggatttg gtgagaatag
     6361 ccgagtgcta aaatgggtat tcgaaagaac atccggtcaa ggcgaggcag taaagacacc
     6421 tattggctat atgcctaccc cggatgctat tgacactgaa ggcttggacg tcagcgaggc
     6481 cgatatggaa gagctcctgg acgttaacaa ggaacaatgg ctgagggaag tagaatccat
     6541 aaaagaatat tataagatgt atgaggataa gttacctcag gaattggccc agcagctgga
     6601 ggccttggaa gagcgtcttc gcagctgaca atttagcgca ttgtgccggt tagacatata
     6661 a
//
LOCUS       DASRNX010000102         6072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_709075,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000102 DASRNX010000000
VERSION     DASRNX010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709075"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VFD89_04035"
     CDS             <1..822
                     /locus_tag="VFD89_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HZJ57391.1"
                     /translation="VYGLPILFPPNRIDGGKFEFSGRTYQFPIGEKRSNNSLHGFLSH
                     RPWGFEVLKSDKDEAVVSLTFKGDKEEDYYSYYPHEFTIKMVYTLNAQGLNQDITISN
                     EGRVDMPFGLGWHTAFALEFKEGINPDDVKVKVSIGGRIQLSDRGLPTGVVSPLSDNE
                     AQFRKEGQSPLYEIMDDHYTVKPIEKDGKPFHGAILEDGTGKARVVYRVDPAYKHWMI
                     WNCFKEGSFICIEPQNWRVNAPNLKLPADESGMEYIVPGGTYRAKASVYVEEDLD"
     gene            894..2087
                     /gene="nagA"
                     /locus_tag="VFD89_04040"
     CDS             894..2087
                     /gene="nagA"
                     /locus_tag="VFD89_04040"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HZJ57392.1"
                     /translation="MGRTLIFNGSIITPYRMIEGGSILIEDGKIAGNYTGKPEIPHCK
                     MVDAKGLYISPGFIDIHTHGAGGHDFMDGTVDAYLGAAMTHAMHGTTALVPTTVASTV
                     DSLKETFETYRKAKSSNIHGAAFLGLHLEGPYFSMEQRGAQDPRYIRNPEREHYEEIL
                     SWSDDIVRWSAAPELDGAMEFGRSLKNRGILPSIGHSDAIYEDVLEAYENGYTHITHL
                     YSGMSGVRRINAYRYAGVIESAFLIDEMTVEIIADGAHLPESLLKLIYKIKGADKIAL
                     ITDSMRAAGMPEGESILGGLKDGQKVLVEDGVAKLMDRTAFAGSVATADRLVRTMINI
                     AEVPLLDAVRMMTITPARIMGVDDKKGSVAMGKDADLVLFDDDIKVHSSMVRGRVVYK
                     CQNPT"
     gene            complement(2104..3081)
                     /locus_tag="VFD89_04045"
     CDS             complement(2104..3081)
                     /locus_tag="VFD89_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZJ57393.1"
                     /translation="MSTQHYNYPLSEKLISSLKKVGLVNFWGPKSLPTVFTSRTLIDI
                     YKQNGTWGTSLFNNVPLFPNENILIYCHDVNQSFWPHRHDFVEIIFVNQGKVINVIDG
                     DHLHMRQGDFCILNPNAEHGLKCPDKNATIVKIALLGPVFQGTLCSFYNDDNPISTLI
                     RGQDTNKENYLYYTSQPNFMVQRIINSIINEYTNSDFQTSFTLEAYLILLFSYLNRQV
                     QCKYYSTDEFTMELLDYIREHCAYQNLDEIAEHFGYTPNYMTRLIKKLTGHNYSQIVK
                     ESRLQASLNYLSNSDLPVNEIAEKVGYNSTSHFFKVFKEYFGFTPSAYR"
     gene            3567..4418
                     /locus_tag="VFD89_04050"
     CDS             3567..4418
                     /locus_tag="VFD89_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZJ57394.1"
                     /translation="MMANRFSVASYSFHGLQGVGAMNIFHYFETVKYRYNLNTADIWN
                     GMLTSYGEKYLKLVKQQMDEHGLDLVNLCCDGAHLWSDDEKEAEFTNKRADECLNAAA
                     ILGAETIRFDVGIGGREATDEQIEYVAKKFDKYCERAARFGAKVGPENHWGASTNIEV
                     MRKLIQAVKAENFGILLHLGNWQMSGVDFEKGCEYDLEMIPHAMYMHIHYEACENAEV
                     QLPPLQEADYTGCWGIESHKSSNEYNNVAYQLSQVKRVVTPLIYKAERREIPEEFQVV
                     IRKMMQK"
     gene            4509..6047
                     /locus_tag="VFD89_04055"
     CDS             4509..6047
                     /locus_tag="VFD89_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HZJ57395.1"
                     /translation="MDILKTRITRTNQGYVQGRAGRDEEVTVFRAVPYAAPPVGPLRW
                     RPPQPPVAWQGVRPAGSESTVPLQERGMFADGGRFDSFYRTEDCLTVDIWTPADTDLD
                     SLPVMIWIFGGRFQGGYASDNMYDSELLAAKGVVCVTVNMRVGVFGYLCHPELLKEGK
                     GLTCGNYGLLDIIEAVKWVSENIKHFGGNPNNITLVGQSTGALSLCRIVASGLLNGLY
                     KGLILQSGDSLLSHKPFEDTMEDAMVRGSEFAKYLGADSIDELRALPPDKFIEDGVNV
                     YEEYFNQICVPIQDRSFFKTPFKESLFTENTRFIPMIIGSNADDGFMIMPSESTNEAL
                     DTILDERFGSFAPKIRRLYSFYGDTQSYQTFRIIERDYWFARIRFIALAREKMRHGAT
                     WQYYFTQPYLWPGLGILGAVHVSELPYVFGSIRLLKGYDYPWDENNDRLFQIMSDFWI
                     AFIKNSNPNKGWLPIWHPRSHRHKYMEIGLDTGMHMEEIMSEEKMKLWTEFCMSYCNY
                     TVGE"
BASE COUNT         1775 a         1180 c         1513 g         1604 t
ORIGIN      
        1 gtctatggac ttcccatatt gtttcccccc aacagaatcg atgggggcaa attcgaattt
       61 tcggggagga cgtatcaatt ccctatcggg gagaaacgta gcaacaattc gctgcatggc
      121 tttttgtccc atcgcccctg gggctttgag gtactgaaat cagataagga cgaggccgtt
      181 gtcagcctga ccttcaaagg ggataaggaa gaggattatt atagctatta tccccatgaa
      241 tttactataa agatggtata caccctaaat gcccaagggc taaaccagga tataaccata
      301 tccaacgagg gcagggtgga tatgcccttt ggcctgggat ggcataccgc ttttgcgttg
      361 gaatttaaag agggaataaa tcctgatgat gttaaggtaa aggtatccat agggggacga
      421 atacagctga gcgatagggg gcttcccacg ggtgttgttt ccccgctgtc ggataatgag
      481 gcacagttta gaaaagaggg tcaatcaccc ctttatgaaa ttatggacga tcactatacc
      541 gtaaagccca tagagaagga tgggaaaccg ttccacggcg ctatattgga ggacggtacc
      601 ggcaaggccc gtgttgttta ccgggtggac ccggcctata agcattggat gatctggaac
      661 tgttttaaag aggggagctt catctgcata gaaccgcaga attggcgggt taatgcgcct
      721 aatctaaaac ttccggcaga tgagtcgggg atggaatata ttgtgcccgg gggtacatat
      781 agggcaaaag cttccgtcta tgtggaagag gatttggact aaggacaatg ggtattgccg
      841 atatgtttaa ctagcataac gggctaaatt atgcacccga ggggggaaag taaatgggca
      901 ggacattgat ctttaacgga tcaattatta cgccctatcg tatgatcgag ggcgggagca
      961 tactcataga agacgggaag atagcgggga actatacggg gaagcctgag ataccccatt
     1021 gcaagatggt agatgcaaag ggcctatata tttccccggg ctttatcgat atccataccc
     1081 atggggccgg ggggcatgat tttatggacg gtaccgtgga tgcatatttg ggggctgcga
     1141 tgacccatgc catgcacggc actaccgccc ttgttcccac cacggtagcc agtacggtgg
     1201 atagccttaa ggagaccttt gaaacctaca gaaaagctaa atcatccaat atccatgggg
     1261 cggcattttt gggactccac ctagagggcc cctatttttc tatggagcaa aggggtgccc
     1321 aggatccccg ctatatccga aatcccgaaa gggagcatta cgaagagatc ctgagctggt
     1381 ccgacgatat agtccgctgg agtgcggctc cggagcttga tggggcaatg gagtttggca
     1441 gatctttaaa gaataggggt atcctgcctt ccataggaca ttccgatgcg atatacgagg
     1501 atgttctaga ggcctatgaa aatggatata cccacatcac ccacctatac tccggaatgt
     1561 cgggggtaag aaggataaac gcatatcgat acgcaggggt tattgaaagt gcctttctga
     1621 tagacgaaat gacggtggaa ataattgcag atggtgcaca tcttcctgaa agtctgctaa
     1681 agctcatcta caagataaag ggagccgata agatcgccct tatcaccgat tccatgagag
     1741 cagccggaat gcccgaggga gaatccattc tagggggctt aaaggatggc caaaaagtcc
     1801 ttgtagagga tggtgtggca aagcttatgg atcggacggc ttttgcgggg agcgttgcga
     1861 ctgccgacag gctggtcagg accatgatta acattgctga ggtgcccctt ttggatgctg
     1921 tccgtatgat gaccattaca ccggctagga taatgggagt agacgataaa aaaggtagcg
     1981 tcgccatggg aaaggatgcc gatctggtgt tgtttgatga tgatattaaa gtccattcaa
     2041 gtatggtgag ggggagggtt gtgtacaaat gccaaaatcc gacctaaggg cagatttatg
     2101 gtatcatcga taagcgctgg gcgtgaatcc aaaatactcc ttgaatacct taaaaaagtg
     2161 acttgtggaa ttatagccaa ccttttcggc aatttcatta acgggaagat cgctattgga
     2221 cagatagttg agagatgctt ggagcctgga ttccttcacg atctggctat agttgtggcc
     2281 ggttagcttt ttgattagcc tagtcatata gttgggggta tagccaaaat gctctgctat
     2341 ttcatccagg ttttgatatg cgcaatgttc tctaatgtaa tctagcaact ccatggtaaa
     2401 ttcgtcggta ctatagtatt tgcattgtac ttggcgattt aaataagaaa aaagcaaaat
     2461 taagtaggct tccaaggtaa aactcgtttg aaagtccgaa ttggtatatt cgttgataat
     2521 agaattaata atacgctgca ccataaaatt gggttgggag gtatagtaca aataattttc
     2581 tttgttcgta tcctggccgc gaataagggt ggagattgga ttatcatcat tatagaaact
     2641 acataacgtc ccttgaaata cggggccaag aagcgcaatt ttaacaattg tagcattctt
     2701 atcgggacat ttcaatccat gctcggcatt ggggttcagg atgcagaaat caccttgtcg
     2761 catatgcaaa tgatcgccat ctatgacatt aatgaccttc ccctgattta cgaaaataat
     2821 ttctacgaaa tcatgccggt gcggccagaa actctgattg acatcatgac aatatataag
     2881 gatattttcg ttgggaaata ggggaacatt attaaatagt gatgttcccc aagtaccatt
     2941 ttgcttataa atatctatga gagttcgtga agtaaatacc gtgggcaagg atttgggacc
     3001 ccaaaaattc accagcccca ccttttttaa tgagcttata agtttctcag ataatggata
     3061 attataatgc tgagtagaca tactcaagac ttacccccta tacaatgatt acttaatcat
     3121 agtttatttc gctatacgca gtgctctatg ctgcctaaat cacaggggca taacctgcca
     3181 taaaacaact gatctagtaa cataaatcaa tacataaata taacggatag ggtcatttcc
     3241 ttacaaggca atatatgact tatattgtct gcgctccccc aaacacggga tagtattata
     3301 cgctgattat agcacatgga ttaaggttag gcaataatgg cacgattttc gacccattta
     3361 cggcattggc ccacccataa ctcatatcct atgaaagaaa agcacacgtt tttgtctatt
     3421 gaacgcattg cgtattctgc ataggatata tataatgtat tcgagcgata acttcatata
     3481 taaaatgacg acttatgcac cttggttatt gaccccataa ggaactgaag ttcaacatta
     3541 aagcaaaaag atataaggag tgtttcatga tggcaaatcg tttttcagta gcatcctact
     3601 catttcacgg ccttcaggga gtaggcgcaa tgaacatctt tcactatttt gaaactgtaa
     3661 agtacagata caacctaaat accgccgata tatggaacgg tatgctaaca agctatggtg
     3721 aaaagtactt aaaacttgtg aagcagcaga tggatgaaca tggccttgat cttgttaacc
     3781 tatgctgtga tggagcccac ctatggagcg atgatgaaaa agaggccgaa ttcactaata
     3841 agagagctga cgaatgccta aacgccgcag ccattttagg tgcagaaact attcgttttg
     3901 atgtgggaat cggcggtagg gaagccaccg atgaacagat agaatacgtg gcgaagaaat
     3961 tcgataagta ttgtgagaga gccgcacgct tcggtgcaaa ggtaggtccg gaaaatcact
     4021 ggggtgcatc gacaaatatt gaagtgatgc gaaagctgat ccaggcagta aaagctgaga
     4081 actttggaat tttacttcat ttgggtaact ggcaaatgtc cggtgtggat tttgaaaaag
     4141 ggtgtgaata cgacttggag atgatccccc atgctatgta tatgcatata cattatgagg
     4201 catgtgagaa cgcggaggtc caattaccgc ctcttcaaga agccgattac acgggatgct
     4261 ggggtatcga aagccataaa tcctctaacg agtataacaa tgtagcctat caactttccc
     4321 aggtaaagag agtagtgaca cccctgatct ataaagcgga acggagagaa atccccgaag
     4381 aattccaagt ggtaatcagg aagatgatgc agaaataatg gactattaaa cctttaattg
     4441 cgtgggaaac cgtatatcgg ctcgcgatcc atgcaaacaa acataaaaaa agtgggcggt
     4501 attttatagt ggatatctta aagactcgta taacgagaac caatcaaggc tatgtacaag
     4561 gaagagcggg aagagacgaa gaggttaccg tatttcgggc ggtaccatat gctgccccgc
     4621 ctgtgggacc cttacgttgg cggccccccc aacctccggt tgcctggcag ggagtaaggc
     4681 cagcaggatc ggaatctact gtaccacttc aagagcgggg aatgttcgcc gatggtggac
     4741 ggtttgattc attctatcgt actgaagatt gcttaactgt tgatatatgg acccctgccg
     4801 acacggattt ggactctcta ccggtaatga tatggatttt tgggggacga tttcagggcg
     4861 gatatgcttc tgataatatg tatgacagcg aactattagc cgctaaggga gtagtatgcg
     4921 ttacagtgaa tatgcgcgta ggagtttttg gatatctgtg ccacccggag ctgctcaagg
     4981 aagggaaggg gctcacatgc ggtaattacg gcttgctcga tataatagaa gcggtaaaat
     5041 gggtatccga aaatataaaa cattttgggg gtaaccccaa taacattaca ttggtcgggc
     5101 aatctacagg agctttgagc ctttgccgca tagtagcttc cggattatta aacgggttat
     5161 ataagggact gatcctgcaa agcggggatt ctttacttag ccataaacct tttgaggata
     5221 ccatggagga tgctatggta cggggcagtg aatttgcaaa atatcttggt gcggattcta
     5281 tcgatgagtt gcgggcgctg cctccggata aattcataga ggacggggtt aacgtatacg
     5341 aggaatactt caatcaaatc tgtgtaccga tccaagatag aagtttcttt aagacgcctt
     5401 ttaaagagag ccttttcacg gagaatacac gatttatccc tatgataata ggttctaatg
     5461 cggatgacgg ctttatgatt atgccctcgg agagtactaa tgaagccttg gatacgattt
     5521 tagatgaacg attcgggtct tttgctccta aaattcggcg attgtactcg ttttatgggg
     5581 atacccaatc atatcaaact ttccgcataa ttgaacggga ttattggttc gcaaggatac
     5641 gtttcattgc cctggcccgg gaaaagatga ggcatggcgc tacctggcaa tattatttta
     5701 cccagccata tttatggccg gggcttggaa tcctaggcgc agtccatgta tccgaattgc
     5761 cttacgtatt tggatctatt cgcttgctaa agggatatga ttatccctgg gacgagaaca
     5821 atgatcgtct atttcaaata atgagtgact tttggattgc ttttataaag aatagcaatc
     5881 ccaacaaggg ctggttgcct atttggcatc caagatccca tcgacataaa tatatggaga
     5941 ttggactaga caccggtatg cacatggaag agattatgag cgaagaaaaa atgaaactat
     6001 ggacggaatt ttgtatgtct tattgtaact atactgttgg tgaataggtt ggtacaaacc
     6061 gttatattat ta
//
LOCUS       DASRNX010000103         9234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_498953,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000103 DASRNX010000000
VERSION     DASRNX010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9234
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498953"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..3605
                     /locus_tag="VFD89_04060"
     CDS             129..3605
                     /locus_tag="VFD89_04060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZJ57396.1"
                     /translation="MAGFVHLHTHTEYSLLDGAGQINDLLDKCKELGMDSLAITDHGV
                     MYGVVEFYKEARSRGIKPIIGCEVYIAPRSMTDRQPRTDNNYAHLVLLAKDQKGYLNL
                     IKLVSMGFIDGFYYKPRIDYEVLSQHSEGLIGLSACLAGDIPRLLLADRKEDAKALAL
                     RLKMMFGEDNFYLELQDHGLEDQVRVNPLIAELSKDTGIPLVATNDVHYVNSGDAPAH
                     DVLICIQTGKTVEDEDRMRFNSLDFYLKSPEEMQELFASYPDAIENTTKIAERCNVEF
                     DFDTLHLPQYDVPEGFTAAQFLRHLCNEGLKIKYDKVTDEARDRLEYEISTIEQMGYV
                     DYFLIVWDFIKFARDNGIMVGPGRGSAAGSLVAYALGITQIDPLKYNLLFERFLNPER
                     ISMPDIDIDFCFERRQEVIDYVVEKYGDDRVAQIITFGTMAARAAIRDVGRALNLPYG
                     DVDRIAKAVPFELGMTISRALEVNGELRELYNAEPVIKNLIDTSRSLEGLPRHASTHA
                     AGVVISKDSITEHVPLQKNDDIITTQFSMGILEELGLLKMDFLGLRTLTVIRDAVQLI
                     KFSQGIEVDINNISLDDVGVYEMLSQADTDGVFQLEGSGMRHFLKELKPSNFEDIIAG
                     ISLYRPGPMDQIPRYIDNKNHPESINYTDPSLTDILEVTYGCMVYQEQVMQIVRELGG
                     YSLGRSDLVRRAMSKKDMDVMERERHHFIYGLSDEDGNITIPGALRNGVSVDQAGKIF
                     DEMMEFAKYAFNKSHAAAYALIAYQTGWLKFHYPVEFMAALMTSVMGNSGKVAGYIQY
                     CRKNGIEVLPPDVNESMAKFSVVGDKIRFGLAAVKNVGISAIEEIVKARDEKGKFRSI
                     LEFCQKVDSSGLNKRLLESLIKCGAFDSMGAYRSQLMAVYERVLDGVLQDRRKNLDGQ
                     ISLFDQSLIQREEPMDFEILPDIKEFPTDILLSMEKEIVGVYISGHPLWEYKDILEGL
                     TTTLDLQSGDFEEGAIVSSGSLRDGARVEVGGIIVEHKRKTTRNNNMMAFVTLEDLYG
                     TIEVIVFPTIYKRYARLLDNDSTVIIHGKLSLREEEEPKILCDRVIPLTSSLNKKLYL
                     KIGTDSRINISNQLNPILKRYSGSIPVILYIESIKQRSLADRELWVNEDRGLLTELSN
                     LLGNGCVKLVQG"
     gene            3701..3937
                     /gene="mtrB"
                     /locus_tag="VFD89_04065"
     CDS             3701..3937
                     /gene="mtrB"
                     /locus_tag="VFD89_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp RNA-binding attenuation protein MtrB"
                     /protein_id="HZJ57397.1"
                     /translation="MDLKELVNDYVCILAKEDGVNIIGLTRGRDTKFHHTEKLDRGEV
                     MIAQFTENTSAIKIRGRAVIYTKHGEITSGNIRD"
     gene            3963..4160
                     /locus_tag="VFD89_04070"
     CDS             3963..4160
                     /locus_tag="VFD89_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57398.1"
                     /translation="MWVVIYMAHRKRDADAIEGILGDENIPYKIKPVYKNIAPEDNCF
                     EILVPQSEAGEVHNILMENGF"
     gene            4192..5151
                     /gene="pfkA"
                     /locus_tag="VFD89_04075"
     CDS             4192..5151
                     /gene="pfkA"
                     /locus_tag="VFD89_04075"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZJ57399.1"
                     /translation="MKTIGVLTSGGDAPGMNAAIRAVVRTAIYNDMRVIGIRRGYNGL
                     INGDLREMDITSVGDIVHRGGTILHTDRSEEFRTPGGQKKAANMAEIFEIQGIVVIGG
                     DGSFKGAYDLCRMGIPVMGIPATIDNDVNSTDFTVGFDTATNTVVDAINKIRDTVTSH
                     QRANVIEVMGRGRGDIALYTGVAGGAEHIVIPEISFKINELCKRLIQGRDRGKSSHII
                     MLSEGMTDVNELCEQIEEKTGIETRATVLGHIQRGGNPTVADRILASKMGYHAVNSLK
                     EGRGNRVVCIRNNQIIDCDLEEGMAMKKRFDTDLYEMSKILSM"
     gene            5275..5697
                     /locus_tag="VFD89_04080"
     CDS             5275..5697
                     /locus_tag="VFD89_04080"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZJ57400.1"
                     /translation="MITVDTEIRVRYKETDRMGIVHHSNYYVWFEIARTEYMRLQGLS
                     YRQTEERGILLPLRETYCKYIQGALYDDQVIIRTSMTNFSGARITMEYEVVRKGDNCL
                     LAKGKTVNAITDDNLKPINIKKIAPDIYQLLLKCVRGI"
     gene            complement(5706..6230)
                     /locus_tag="VFD89_04085"
     CDS             complement(5706..6230)
                     /locus_tag="VFD89_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZJ57401.1"
                     /translation="MNNKAGMRSLFIVFILAIAITGCTVTKKPPAKKGETPKSSQSEN
                     APTEQEKQEQTPTNEEKLDGPTTAKDVKDGKYEGETDKDERGNFGEAKITVKDGKITE
                     AEYTEFTEDNKPKTKESGYDYQQALDAFKKLPDILVEKQDLTKIDTYAGATGTTKTFK
                     IAVMRALEKGTKEK"
     gene            complement(6610..9114)
                     /locus_tag="VFD89_04090"
     CDS             complement(6610..9114)
                     /locus_tag="VFD89_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HZJ57402.1"
                     /translation="MEKTTCCNTSKKKGFKLNPELGYFQNIGVDVMAFDDIYPEGHQS
                     GVSIIMHGNRVATNGDIRFEQTPGQWQPVPKQGERKLDEATNTITTSLSYPDTSRHLR
                     GFNPMIYPDFEFNYEVIVEGRDDSVMVTVNLDRPIPEVFVGKMCFNMELFPGALFGKP
                     WIMDNKQGIFPEQPNSPLLTLPPNYLHTGDFSEPDATADIKRLSGDNKGYNPIIADDI
                     IAEPYAVGKRFVVRPDDPQNRVVIESKGTELKLYDGRMNHNNGWFVVSSEVPADATEG
                     AIQWIITPNVVEDWLYPAVVQTSQVGYHPDQPKTAIIELDRRDGRSENPVLLKITETG
                     EKQVYSNAGNRWGQFLRYNYLKFDFSHIEEEGLYKVRYGTSESAIFRIAQDVYDRGVW
                     QPVLEYFLPVQMCHMRVNEKYRVWHGLCHDDDAQMAPVNYNHIDGYVQGPSTLTKYKP
                     GDVVPGLNVGGWHDAGDFDLRVESQSGEFYILTLAYEAFDVNYDVTTIDQERRVTEIH
                     QPDGKNDILQQIEHGVLSVVGAYRALGRLYRGIICNDLRQYVLLGDSAAMTDGIKGNA
                     DDRWVFTEDNPPRELTTAAHLAAASRALKGFNDTLSDQALEAAREIFKVTDGTDKARA
                     PKIHAAVELLLTTGEDEYEDFLLSEIDFIVDSIEHVGWFTARAEKVMKNAEFSRKFHD
                     ALPGLKKRFDEQSAETPYGIPYRPHIWGAGWGIQSLGYRYYFLHKAYPEIFDPEMIFN
                     ALNFILGCHPGSNTVSFASGIGSQSATVGYGLNRADWSYIPGGVISGTALIRPDFPEL
                     LVFPYLWQQVEYVMGGGSSHYMFLVLAAQQITGDEE"
BASE COUNT         2531 a         1920 c         2308 g         2475 t
ORIGIN      
        1 caaatatggc cgcccaattt cttgatatgt ctaattgtat cgtagaagga aaaatgatag
       61 aataggttaa aggattttct aaggattatt acggcatatt atattaccat tatttagggg
      121 tgagaaaatt ggctggtttt gttcatcttc atacccatac cgaatatagt ctcctagacg
      181 gagccgggca gattaatgat ttgctggaca agtgtaaaga attgggaatg gatagtctgg
      241 cgatcacgga tcatggggtc atgtatgggg tggtggagtt ttacaaagag gcaaggtcca
      301 ggggcataaa gcccattatc ggatgcgagg tctacatagc accccgctct atgaccgaca
      361 ggcagccccg taccgacaat aattacgccc atctggtact tctggccaag gaccaaaagg
      421 gttatctcaa cctgattaag ttggtatcca tgggttttat tgatggattt tattacaagc
      481 ccaggataga ctatgaggta ttatcccaac acagtgaagg cttaattggt ctcagcgcct
      541 gcttggccgg ggacattccc aggctactat tggcagaccg gaaagaggat gccaaagccc
      601 tggcattgag attaaaaatg atgtttgggg aggacaactt ctatctggaa ttacaggatc
      661 atggtttgga agatcaagtt agggttaatc cgctaattgc ggaactgagt aaggataccg
      721 gtattccatt ggtagcaacc aacgatgtac actatgtaaa ctccggtgat gcgccggccc
      781 atgatgtctt aatatgcata cagaccggga aaaccgtgga ggacgaggat agaatgagat
      841 ttaattctct ggatttctat ctaaaatccc cggaagagat gcaggaatta ttcgcctctt
      901 atcccgacgc aatagaaaat accactaaaa ttgcagagcg ctgtaatgta gaattcgact
      961 ttgataccct gcatttacct caatatgacg ttcctgaggg tttcaccgcc gcccaattct
     1021 tgaggcacct atgcaatgaa ggtcttaaaa taaaatacga taaggtgacc gatgaagcga
     1081 gggatcgcct tgaatatgag atcagcacca tagagcagat ggggtatgtg gactattttt
     1141 tgatagtatg ggactttata aagtttgccc gggataatgg gatcatggta gggccgggtc
     1201 gaggcagcgc tgcggggagt ctggtcgcat atgctctggg cataacccag atagaccctc
     1261 taaagtacaa cctgctcttt gaaaggtttc taaatcccga gcggatcagc atgcccgata
     1321 tagatattga tttttgcttt gaaagaagac aagaggtcat agactatgtg gtagaaaagt
     1381 acggggatga tcgggtggcc caaataataa cctttgggac catggcggcc agggcggcca
     1441 tacgggatgt aggcagggcc ctcaacctcc cctatgggga tgtggaccga atcgcaaagg
     1501 ccgtaccctt tgaattgggg atgacaataa gcagggccct agaggttaat ggggaactca
     1561 gagagctata taatgccgaa cctgtcataa aaaacctaat cgatacctcc cgcagccttg
     1621 aaggattacc gagacatgcc tctacccatg cggcgggcgt ggttatatcc aaggactcta
     1681 taacggagca tgtcccgctc caaaaaaatg atgatattat taccacccaa ttctctatgg
     1741 gtatacttga ggaattgggg cttctgaaga tggacttttt aggcttgcgc accctaaccg
     1801 taatcaggga tgcggttcag ctgattaagt tcagccaggg tatagaggtc gatattaaca
     1861 acatatccct cgatgatgtt ggggtatacg agatgctcag ccaggcagat accgacggtg
     1921 ttttccagct ggagggttca ggcatgaggc atttccttaa ggaactaaaa ccaagcaatt
     1981 ttgaagacat cattgccggg atatccttgt atcggccggg gcctatggat cagataccca
     2041 ggtatataga caacaagaat catcccgaat ccattaatta cacggatccg agcctgactg
     2101 atattttaga ggttacctac gggtgtatgg tctatcaaga acaggtaatg caaattgtaa
     2161 gggagctggg cggctattct ctgggcagat cggatcttgt ccggcgcgct atgagtaaga
     2221 aggacatgga tgttatggaa agggaaaggc atcactttat atatgggctc agtgatgaag
     2281 atgggaatat aactattccg ggcgccctta gaaatggtgt gagcgtggac caggccggta
     2341 agatatttga cgagatgatg gaattcgcaa aatacgcctt taataagtcc catgcagccg
     2401 catatgccct tatagcttat cagaccgggt ggctaaagtt ccattatccc gtagaattta
     2461 tggctgctct gatgaccagc gtaatgggaa acagcggcaa ggtggcagga tatatccaat
     2521 actgcaggaa aaacgggatc gaggtgttac cgcccgatgt aaacgagagc atggctaaat
     2581 tttctgtggt tggtgataag atcagatttg gtctggccgc tgttaagaat gtaggtattt
     2641 ccgcgataga ggagatagta aaggcgcggg atgagaaggg caagtttagg agcatcctag
     2701 aattctgtca aaaggtggac agctccgggc tcaacaaaag actgctggaa agcctgataa
     2761 aatgcggtgc ttttgattcc atgggggctt ataggtccca gcttatggct gtctatgaaa
     2821 gggtgcttga cggtgtactc caggatcgca gaaaaaatct ggacggacaa atatccctgt
     2881 ttgaccaaag cctgattcaa agggaagagc caatggactt tgaaatcctt cccgatatca
     2941 aggagtttcc cacagacatc cttctcagta tggaaaagga gatagtgggg gtgtatataa
     3001 gcggacatcc cttgtgggaa tacaaggata tactggaggg gcttaccaca acccttgacc
     3061 ttcaatcggg ggattttgag gaaggtgcca ttgtatcgtc ggggtcttta agggacggtg
     3121 ccagggtgga agtcggggga ataatcgttg agcacaagcg caagactaca cgaaacaata
     3181 atatgatggc ctttgtcacc ttagaggatc tctatgggac tattgaagta attgtatttc
     3241 ccaccatata taaaaggtat gcaaggctac tggataacga cagcaccgtg ataatccatg
     3301 gcaagttgag ccttagggag gaagaggaac ccaagatttt gtgtgacagg gtgattccct
     3361 tgacatcctc tttgaacaaa aagttgtatt taaagatagg gaccgatagc aggatcaata
     3421 taagcaatca attgaaccct atattgaaaa gatacagcgg gagtattccg gtgatcctct
     3481 atatcgaatc cattaaacaa agaagcctgg cggatcggga attatgggtg aatgaggata
     3541 ggggattgct aactgagctc tcaaacctgc tgggaaatgg gtgcgttaaa ctggtacagg
     3601 gttagttata agcggtgatg taccgccggg ttgcaatgat gctatgtgat tttaattttg
     3661 caggggtacc gggtattgtg tatttaggag gttgaacgta atggatttaa aggagttagt
     3721 caatgattat gtgtgcatac tggccaagga ggacggggtg aatattatag gcctcacccg
     3781 aggtagggac acaaaatttc atcatacaga aaagctcgat aggggtgagg ttatgatagc
     3841 ccagttcaca gagaatactt ctgccataaa gattcgcggc agggctgtca tctataccaa
     3901 gcatggcgaa ataacctcag gaaatataag agattaaacg atatctcata ggagggctta
     3961 taatgtgggt tgttatatat atggcgcatc gtaagaggga tgccgatgcc atagagggta
     4021 ttttggggga tgagaatata ccgtacaaaa taaagcctgt ctacaagaat atagcccccg
     4081 aagataactg ttttgagata ttggtccccc aatctgaagc cggggaagtc cataacatat
     4141 tgatggagaa tgggttttag gtccatattt tttgtgagga ggtagttggg tatgaaaaca
     4201 ataggggtct tgacaagcgg cggggatgcc ccgggaatga atgccgcaat aagggctgtg
     4261 gtccggactg ctatctacaa cgatatgagg gttattggta tccgtagggg gtataacggt
     4321 ctaataaacg gcgatctccg ggagatggat attacctcag ttggggatat cgtacacagg
     4381 ggcggtacta ttttgcatac ggatcgctct gaggaattta ggacgcccgg gggtcagaag
     4441 aaggccgcca atatggcgga gatatttgaa atccagggta tagtggttat cggcggcgat
     4501 ggttccttca agggagccta tgacttgtgc cgaatgggaa tacccgttat gggaattccg
     4561 gctaccatag acaacgatgt caattctacg gattttaccg tgggctttga tacggcaacc
     4621 aatacagttg ttgacgctat aaataaaatt agggataccg taacgtccca tcagcgagca
     4681 aatgtgattg aggttatggg acggggccga ggtgatatcg cactctacac cggggttgca
     4741 ggcggagcag agcacatagt tatacccgag atctccttta agatcaatga actatgcaag
     4801 cgtcttatcc agggcagaga ccggggaaag agttcgcata ttattatgct ttcagagggt
     4861 atgaccgatg tgaacgaatt atgtgaacag attgaagaaa agacagggat cgaaactagg
     4921 gctacggtgc tggggcatat ccagcgaggc gggaatccta cggtagccga taggatactg
     4981 gccagcaaaa tgggttacca tgccgtgaat tcgttaaaag agggcagggg taatagggtt
     5041 gtgtgtataa ggaacaacca gataatcgat tgtgatttag aggaaggtat ggccatgaaa
     5101 aaacgctttg ataccgacct ttatgagatg tcaaagatac tgtccatgta gacgattaga
     5161 ttttgggatt gctcaaaatg caaaaagtct agtagcctat aacggcttgc ttaggggata
     5221 tcgtttacct cattgtaata caactgaatc catacggtat aggagatggt taaactgatt
     5281 acggttgata ccgaaattcg tgtcagatat aaggaaacgg ataggatggg gatcgtccac
     5341 cattccaact actatgtctg gtttgaaata gcaaggaccg agtatatgag gctgcagggc
     5401 cttagctaca gacagactga agagcggggc atcctgctcc ctttaaggga aacctattgt
     5461 aaatacattc agggagccct atacgatgac caagttatca tacgaacatc catgacgaat
     5521 ttttcaggag cgagaattac aatggaatat gaggtggtaa gaaaagggga caattgccta
     5581 ttggcaaagg gtaaaaccgt caacgccata acggacgata acctaaagcc aataaacatt
     5641 aagaaaattg cccccgatat ctaccaactg ttactaaaat gcgttcgtgg catatagcgt
     5701 catagttatt tttctttggt ccctttttct agggctctca ttacagctat cttaaaggtc
     5761 ttggtagtac ctgttgcgcc tgcatatgtg tctatcttgg tcaggtcctg tttctcaacc
     5821 aggatatccg gcaatttttt aaatgcatcc aaggcttgct gatagtcata cccgctctcc
     5881 tttgtttttg gcttgttgtc ttcggtgaac tcggtatatt ctgcctcggt tatttttccg
     5941 tcctttacgg taattttggc ttcaccaaaa ttccctcgtt cgtctttatc ggtttcacct
     6001 tcgtactttc cgtcctttac gtctttggca gttgttgggc cgtccaactt ctcttcgttt
     6061 gtaggggttt gctcctgttt ttcctgctct gtaggcgcat tttcggattg gctggactta
     6121 ggtgtttctc cctttttggc agggggtttt ttggtcaccg tacagccggt aatggctatg
     6181 gctaggataa aaactatgaa taaagaacgc atacctgctt tattgttcaa cttaattccg
     6241 cctttcgatt ttttccaaat tggttctaat gctatagctc atattgctga gcccgacaca
     6301 tattatttcg ataagtcgtc aaaaacgccc agccgatcta aagggcaggt acttctgata
     6361 atacatatag cataaccaat tttaatgaaa aattgcgtga aagttcggat tggaagccat
     6421 aggctcttaa ttgaaatacg cgtaagtttc cccgaccatt attgatatat ggttaatcag
     6481 tggaacatat taaatcagcc cttgcgcagt tgtgggcgca agggctgatt cttgatctaa
     6541 ctataaacca ttggaaagcc gccatttata gaacatgaat ggaggttttt ccgcatcgtt
     6601 ataactgctt tattcttcat ccccagtaat ctgttgtgca gccaacacta agaacatata
     6661 atgggaagat cctccgccca ttacgtattc tacttgttgc cagagatagg ggaataccaa
     6721 caattctggg aaatcgggtc ttataagtgc ggtacccgag attaccccgc cgggaatata
     6781 ggaccaatcg gcacgattga gtccgtagcc tacggtggcg gattgggatc ctatccctga
     6841 agcaaaggat acggtattag agcccggatg acagcctaag ataaagttta aggcgttaaa
     6901 tatcatttcc ggatcgaata tttcgggata agccttatgc aggaaatagt atcgatatcc
     6961 cagactctgg ataccccaac ctgctcccca tatatggggg cgatagggaa ttccgtaggg
     7021 ggtctcggca ctctgctcat caaaccgctt ctttaaccct ggcaatgcgt catggaattt
     7081 cctcgaaaac tcggcatttt tcatcacttt ttcagcccgt gcagtaaacc agccgacatg
     7141 ctctatggag tcgactataa aatcgatttc cgatagcaaa aagtcctcat attcatcttc
     7201 gccggtggtt agaagcaatt ctactgcagc atggatcttt ggggctctgg ccttatctgt
     7261 tccgtcagtg accttaaaga tctcacgggc ggcttcaagg gcttggtcac tgagggtatc
     7321 gttaaaaccc ttcagtgccc gggaggcagc agccagatgg gctgcagttg tcagttctct
     7381 ggggggatta tcttcggtaa atacccagcg atcatcggca tttcccttga taccgtcggt
     7441 catggccgca ctgtcgccaa gaagcacata ttgtctcaga tcgttacaga taataccccg
     7501 atacaaccgg cctaatgcgc gataggcacc aactacggat aaaacaccgt gctcaatctg
     7561 ttgcagaata tcattttttc catccggctg atggatttcg gttactcgcc tttcctgatc
     7621 tatggtagtt acatcataat tcacatcaaa ggcctcatat gccaaggtta ggatgtaaaa
     7681 ttcaccggat tgcgattcca cacggagatc aaaatcgccg gcatcatgcc agcccccgac
     7741 attaagaccg ggaacgacat ccccaggctt gtatttggtc aatgtggaag ggccctgtac
     7801 atatccatcg atgtgattat aatttaccgg agccatctgt gcgtcatcat cgtggcatag
     7861 tccatgccag acccgatatt tttcattaac acgcatatgg cacatctgta caggaaggaa
     7921 atattccagc acaggctgcc aaacgcctct atcataaaca tcctgggcga tacggaagat
     7981 ggctgattcg gaagttccgt accggacctt gtataggccc tcttcttcta tatgggaaaa
     8041 atcaaatttt aaatagttat agcggaggaa ttggccccac ctgttaccgg catttgagta
     8101 gacctgtttc tcgccggtct ccgttatctt aaggagaacc ggattttcgc tcctgccgtc
     8161 gcgcctatca agctcaatga ttgccgtctt gggttggtcg gggtgatatc ctacctgtga
     8221 tgtctgaaca accgccggat atagccagtc ttcaacaaca tttggagtaa taatccattg
     8281 aattgctcct tcggtggcat cggcaggtac ttcgctgctg acgacaaacc aaccgttatt
     8341 atggttcatg cgcccgtcat agagtttaag ttccgtaccc ttgctttcaa ttacaactcg
     8401 gttttgagga tcgtcgggcc taaccacaaa ccgcttaccc acagcatagg gctcggcgat
     8461 tatatcatcg gcgataatag gattgtatcc cttgttgtcc ccagatagcc gtttgatatc
     8521 tgccgttgca tcgggttcac taaaatcccc ggtatgtaga tagttagggg gcagagtcag
     8581 caaaggactg ttgggttgct ccgggaatat gccctgttta ttatccatta tccaaggttt
     8641 tccgaataat gctccgggga agagttccat attaaaacac attttcccca caaacacttc
     8701 aggtatcggc ctatccaaat taacggtcac cattacggaa tcgtccctac cctcaacaat
     8761 aacctcgtaa ttgaattcaa aatctggata gatcattgga ttaaagcccc gaaggtgcct
     8821 ggatgtatcc ggatagctca gtgaagtcgt gatggtgttg gtggcttcat ccagcttgcg
     8881 ctcaccttgt ttcggtacgg gttgccattg tcccggtgtc tgctcaaagc gtatatcacc
     8941 gttggttgca actcgattgc cgtgcatgat tatactcaca ccggattgat ggccctccgg
     9001 gtaaatatca tcgaaggcca taacatctac gccgatgttt tggaaatagc ccaattcggg
     9061 atttaattta aatccctttt tcttggaggt gttacagcag gtcgtctttt ccattttctc
     9121 ttccaactcc ttgtctattc ttaattgcaa tgtaccaatc cagtcatctt atgggattta
     9181 ggcctattgc ggaataaagt tatagtggga caatacttta gcacattaat atac
//
LOCUS       DASRNX010000104         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_179283,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000104 DASRNX010000000
VERSION     DASRNX010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179283"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /gene="tnpA"
                     /locus_tag="VFD89_04095"
     CDS             complement(<1..382)
                     /gene="tnpA"
                     /locus_tag="VFD89_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HZJ57403.1"
                     /translation="MPKTMRWRGYVYYIKYHIVWCTKHRHKIIQGDIEIFLKELLYRI
                     AKDNGFSIEVMETDKDHIHLLVNCSPQHHIPSIIKALKGASARALFKKMPELKNKLWG
                     GHLWNPSYFVATVSENTESQIKEYI"
     gene            complement(844..1584)
                     /locus_tag="VFD89_04100"
     CDS             complement(844..1584)
                     /locus_tag="VFD89_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57404.1"
                     /translation="MKRLFILLFTSILIASLIGCANSTVPQKRVPPKPEGNPVKKSDP
                     PADPPKPSGESVPGPWPDRIEIKTGKKVKVNTYSIEGETVYIDPKGKETRGKITMVLK
                     NTKGKWTVSQFDTEQENKSSLVYKNTEFGFSFNLPLTWKGYRIIEGGWESNDKAQSGP
                     SISIRHPKWTEDVPRQDIPIMIFTHEQWDLVEKEKLAVGAAPIGPRMLVENKKFVFAL
                     PPRYNFSFLEGFEEVEKIIEDNPLKAND"
     gene            1859..2710
                     /locus_tag="VFD89_04105"
     CDS             1859..2710
                     /locus_tag="VFD89_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZJ57405.1"
                     /translation="MKSLTDTYTLSNGVGIPCIGFGTWQTPDGEVAVSSVLAAIEAGY
                     RHIDTAQGYGNEESVGIAVKKSGVKREEIFITSKLANSEHGYEKTLAAFEGTMEKLDM
                     DYLDLFLIHWPNPIAFRDNWQEANAGTWKAFEELYNAKRIRAIGISNFHPHHIDELMK
                     TATVAPMVNQIRLCPGDTQDEVVDYCRNRNILLQAYSPFGVGKIFEVPEMKDLAKKYG
                     KSIAQIAIRWSLQRGYLPLPKSVTPARIKENVDVFDFELEESDVELIANLKGCVGYST
                     HPDSITW"
     gene            complement(2818..>3049)
                     /locus_tag="VFD89_04110"
     CDS             complement(2818..>3049)
                     /locus_tag="VFD89_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57406.1"
                     /translation="FYGSGSENVGVQGYDMVTRLGSNIGFTEITASLRNTDNIEILNQ
                     NFGLDLRDGIDSVTLNPEIPHYYLDIKPVDKK"
BASE COUNT          821 a          659 c          628 g          941 t
ORIGIN      
        1 taatatactc tttaatttga gattccgtat tctccgatac tgttgctaca aaatagctgg
       61 ggttccatag atgtccgccc caaagcttgt tttttagctc tggcatcttt ttaaataagg
      121 ctctggcgct ggcacctttt aaagccttga ttatgctagg tatatgatgt tgtggtgagc
      181 agttaacaag caaatgaata tggtctttat cagtttccat aacttctata gaaaagccat
      241 tgtctttcgc tatcctatat aataattctt ttaagaatat ttcaatatcc ccttgtatga
      301 tcttgtgtct atgcttagta caccatacaa tatgatactt aatataatag acgtatccac
      361 gccagcgcat tgtctttggc atgatactca tccctgcata tattatacca cgcgctggcg
      421 gaaaacacac taaataatcc actaaaatta agattcaggc cactaactca cgactgaagt
      481 cacgagtatg cgtggccaat caatcaataa cgaaaagttt tatcggtact cttaagacaa
      541 aagtcaatat cgtctgctgt tatcttcgga tatggacatt atagcatatg atgtggggat
      601 gaatcatcaa gtatatgata tttttggtat aaatgacctc gtacatgtta tgcatggaaa
      661 agccactaat catgggttgg attccgggat tagtggctat actgtaagaa ctttctgcct
      721 atcgtctttg cagaatgagg ctgacatcta tgtttctctg ggcaaataga gaaatctatc
      781 gtctgcatta ccgccaggag taaaatagat atcccaattt tcatgctctt ttatctcatt
      841 acatcagtcg ttggccttta gcgggttatc ctctatgatt ttttctacct cttcaaagcc
      901 ctcaagaaag gagaaattat atcgtggggg cagggcaaat acaaattttt tattctccac
      961 cagcatcctg ggccctatgg gggctgctcc tacggctaat ttttctttct cgaccagatc
     1021 ccattgttcg tgagtaaata tcatgatggg aatatcctgc cttggtacgt cctcggtcca
     1081 tttcggatgc ctaatgctta ttgaaggacc tgattgggcc ttatcgttac tttcccagcc
     1141 cccctcgatt atcctatatc ctttccatgt caggggtagg ttaaagctaa agccgaattc
     1201 agtgttttta taaaccagtg aactcttatt ttcctgctcg gtatcaaact gactaacagt
     1261 ccattttccc ttagtgtttt ttaggaccat ggtgattttt ccccttgttt ctttaccctt
     1321 gggatcgata taaacagttt ccccttcaat agagtacgtg tttaccttta ccttcttacc
     1381 tgtctttatc tcaatcctgt caggccatgg accggggacg gattcccccg atggtttggg
     1441 cggatcggcc ggcgggtcac ttttcttgac gggattccct tccggtttgg gcggtaccct
     1501 cttctgtggc accgtactgt tggcacaccc tattaacgat gctatcaata tcgatgtaaa
     1561 caacagaatg aataatcttt tcatctttgc atcccctttg aattaagata gtggatcatc
     1621 attagtatgg gccaattccc aataaaattt gcattcctag ggataatttg tgtacgtccc
     1681 ttttaatggt ctcctttgac catctgtagt ggcccgggcc ggcaatttga atacattcct
     1741 tttagtctct attgtcctat ttttttgatt caatgttaac aataaggttt ataggtatta
     1801 caagacgatt ttcggaccaa atcaagaaac ccactatttt aaggaggcat gtattattat
     1861 gaaatcacta accgatactt acactctgtc caatggggta ggaatcccct gcataggctt
     1921 tggtacatgg caaaccccgg atggggaagt agccgtttca tccgttctgg ctgctattga
     1981 agcaggatat cgccatattg acacggcgca gggttatgga aacgaggaga gcgttggtat
     2041 agccgtaaag aaaagcgggg taaagcgaga ggaaatcttc atcacgtcca agctggctaa
     2101 cagcgagcat ggctatgaaa agacattggc tgcttttgaa gggacaatgg aaaagttgga
     2161 tatggattat cttgacctct ttctaatcca ttggcctaac cccatcgcat tccgtgataa
     2221 ctggcaagag gccaatgcag gtacatggaa ggcttttgag gaattatata acgccaaacg
     2281 cattcgagct atcggaatca gcaattttca cccccaccat atcgatgaat tgatgaagac
     2341 agccaccgta gccccgatgg taaaccagat acgcctctgt cctggggata cccaagatga
     2401 agtagtcgat tactgccgca accgaaacat tttactgcag gcctatagcc cctttggtgt
     2461 cgggaaaata ttcgaagtgc cggaaatgaa ggacttggct aagaaatacg gtaaatctat
     2521 cgctcagatt gccattcgct ggagcctgca aaggggatac ttaccgctgc cgaaatccgt
     2581 tacaccagca agaatcaagg aaaatgtgga cgtgtttgat tttgagcttg aagagtcgga
     2641 tgtagagttg attgccaatc taaagggttg tgtcggttac tccactcatc cggattctat
     2701 cacttggtaa atctagacac aaaatcgatt tgacactgca aaaggactgg tcattgatct
     2761 ctatcaatta tcagtccttt ttattgtggg gtaattgggg gcgtacatta ttcatcacta
     2821 tttcttatcc accggtttaa tatctaaata ataatgggga atctccgggt ttaaagtaac
     2881 actgtctatc ccgtctctta aatcaagccc aaagttctga tttaatatct caatattgtc
     2941 ggtattgcga agagaagcgg ttatttcggt aaaccctata ttgctcccca agcgggttac
     3001 catatcgtag ccttgtacgc ctacgttttc agaaccggaa ccataaaag
//
LOCUS       DASRNX010000105         7046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_551361,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000105 DASRNX010000000
VERSION     DASRNX010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551361"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            453..1556
                     /locus_tag="VFD89_04115"
     CDS             453..1556
                     /locus_tag="VFD89_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ57407.1"
                     /translation="MKKYRVGVIGFAHMHIVSLVKDFVGLGERVEWVACSDVKPATPS
                     ISEKPSTRAHNIKAVMDMTKMDTLYEDYKQLLDECDLDIAIVCSENALHGKVVSEVLL
                     RGIHVVVEKPMATSMTDALGMVSAAKQGNAVLAVNWPSTWMPGVRLAHKLTSEGVVGK
                     VFKFTYRNSDSQGPFSYGEGLTDLEKGHEWWYNAKTGGGAFWDYCCYGSCLASWFIGT
                     PIRSAYGLRANFDSPYGNAEDYGTITARFDDAVALIEGSWTTVNRGVPSGPIVFGLEG
                     TLVTDGDEVRVYDERHTTAPSSVHQAEPIPEDRDNIAKEFISHLENGTPLHPTLDIET
                     NLNAMMILDTGYRSSMSGKMEQAPGHHYAPAHL"
     gene            complement(1741..4401)
                     /locus_tag="VFD89_04120"
     CDS             complement(1741..4401)
                     /locus_tag="VFD89_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor GTPase family protein"
                     /protein_id="HZJ57408.1"
                     /translation="MKRLVMGILAHVDAGKTTLSEGMLYLSGKTRKLGRVDNKDAYLD
                     THGLERKRGITIFSKQAVFEMGGTEITLLDTPGHVDFSAEMERTLQVLDYAILVISGA
                     DGVQGHTETLWNLLAMHKIPVFLFINKMDQEGADKEKLMAELKRSLSDGCIDFEGIGT
                     EDFYEDLAMCDESVMESYLETGNIKTSQIAGLIKGNKVFPCFFGSALKVEGIQRFMDG
                     IAEYADEPSYPKAFGAKVFKISRDEQDNRLTYMKITGGEIKIRDPLTNGIWEEKVNQI
                     RIYSGEKFDMVDRAEAGTICAVTGLSQTRPGEGLGIERDSKAPLLEPVLSYQILLPEG
                     LDPRAMLPKLRLIEEEEPELRIVWDEELKEIQIQIMGEVQTEILGDIIKDRFGVEVSF
                     DAGKVVYKETIDNRVVGVGHFEPLRHYAEVHLLLEPGKRGSGLRFSSQCSEDLLGRSW
                     QNLILSHLEEKSHKGVLTGSEITDMKITLVNGRAHKKHTQGGDFREATYRAIRQGLKQ
                     AESVLLEPYYSFRLELPEKMVGRAMADIDQMQGSWQIVHSDGERVTLSGDAPVVAMKN
                     YHKEVMAYTGGYGRLFCSFKGYKPCHNSEEVIESIGYDSERDIDNPTGSVFCANGVGF
                     LVDWDRVPDYMHIESYFQDRGEYPLNVNEAAVAKASSKAEAWIDPDEVDEIINRTYFA
                     NRGKKSAWKRRKPTKESNYRPIVRTMERAESKEEYLLVDGYNIIHAWPELKTLVDDDH
                     MEAAREKLQDILSNYQGIRRTHIILVFDAYRVPGRREEIIDYNNIHIVYTREAQTADH
                     YIEKFVHDHHKKYRITVATSDAVEQMIIRGKGAALMSARELREEVHWVVSAAIQAYRE
                     KYGVQPNYLVDLLSGDTKDYMTGLLDGDEK"
     gene            4828..6342
                     /gene="putP"
                     /locus_tag="VFD89_04125"
     CDS             4828..6342
                     /gene="putP"
                     /locus_tag="VFD89_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010651634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HZJ57409.1"
                     /translation="MGGNNLHGSTVLMFLFYLAFMMIVGVYFYRKSKDDDSLSGYLLG
                     GRNLNPLVAAISAGASDMSGWLLMGLPGAAYLAGVSAGWIGIGLAIGTWFNWTFVAKR
                     FRIYTEVSGDSVTVSDFFENRFRDTSHILRVVSAFFIVFFFLIYTASGFVAGAVLFNT
                     VFGLSYINGLILGSVVILIYTVLGGFLAVSWTDLFQGLLMIIALVGVPLGVISSIGGP
                     QVSIMSAKAINPHVLNIFKNPDGTSLGFWATASLLAWGLGYPGMPHIITRFMAIRDAD
                     ETKKARHIAIFWVFLGLFCAVLIGVVGMTYLKDSPLQGTDVEKVFMILIQSIFHPAIA
                     GIFLAAVLAAVMSTVDSQLLVASSSMTKDFYQVFLKKDAGDKELVIFGRIAVAVISII
                     AFVIALNPDSKVLDLVSYAWAGLGATFAPIVVVSLYWRDMNRHGAFAGMLVGGITSIV
                     WNKLSGGIFDIYELLPGFLFAFIAIIVVSKLTGGAQQEIKDEFDKVESMLIKKS"
     gene            complement(6915..>7046)
                     /locus_tag="VFD89_04130"
     CDS             complement(6915..>7046)
                     /locus_tag="VFD89_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZJ57410.1"
                     /translation="KNIMLTLSEREWVCSSCGTRLDRDINASVNIRNEGLNCLGQTA"
BASE COUNT         1714 a         1690 c         1521 g         2121 t
ORIGIN      
        1 gttgaaacct gtgtgtagca tttgctagtg caattgtttc caatgttata agggtttaat
       61 gatgcgggag atctatagcc atattgcttg tctaattggt ggtgagccgc tgattattaa
      121 agcaagggat agtctcccgc accgttatac ccaaaatcct atattatctg gcaaaattta
      181 tactccgtta tcggcccggt ttagcctgta aaacggatca acagaaatga atttgtgcgg
      241 tgctgatggt ttatccggtg atgctttaat acatttgaca ggaattgcct tccatactac
      301 aataattatg agattatggg taaacaatac gaaaaattga atttatcgcc ttgatgatta
      361 caatggaccc atacagtttt agaccataag ttttgtaggc agagttgttt atccgtaaat
      421 atgctaaata atactggaaa ggggttcttc ggatgaaaaa gtatcgtgtc ggtgtaattg
      481 gatttgcaca catgcatatc gtgtcgctgg ttaaggactt tgtaggcttg ggtgaaagag
      541 tggagtgggt tgcctgttcg gatgttaagc cagctacccc ctctataagt gaaaagcctt
      601 ctaccagggc ccataatatc aaggctgtaa tggacatgac caaaatggac accctttatg
      661 aggattataa gcagctgttg gacgaatgcg atttggatat cgccatagtc tgctccgaga
      721 atgcgcttca tggtaaggtg gtatcggagg ttttacttag gggtatccac gtggtggtag
      781 aaaagcccat ggctacctcc atgactgacg cgctagggat ggtctccgcc gcaaaacagg
      841 gcaatgcagt gcttgccgtt aactggccct ctacatggat gccgggagta agactggctc
      901 acaaactaac ctctgaagga gttgtaggca aggtctttaa atttacatac cgcaacagcg
      961 attcccaagg acctttttcc tacggcgagg gtctgacgga tctagaaaaa ggccacgaat
     1021 ggtggtacaa tgccaagact ggtggcggcg ccttttggga ttattgttgc tacggttctt
     1081 gcctggcaag ttggtttatc ggcaccccta taaggtccgc atacgggctt cgggcgaact
     1141 ttgacagccc ctacggcaat gccgaagact atggcaccat caccgcaagg ttcgatgacg
     1201 cagtggcctt gattgaaggc agctggacta cggttaacag aggcgtacct tcgggtccta
     1261 ttgtctttgg tctggagggt accctagtaa cggacggcga tgaggtaagg gtctatgacg
     1321 agcggcatac taccgcaccg tcctctgtac atcaggcgga acctatccct gaggatagag
     1381 ataatattgc caaggaattt ataagtcatc tggaaaatgg cacgcctctg catcccacac
     1441 tggatattga aacgaatctg aatgccatga tgatattgga caccggatac aggtcctcta
     1501 tgagcggaaa aatggaacag gccccgggac atcactacgc tcccgcacac ctgtaaaggt
     1561 gggccttgac taggatttat tccccataaa ctaaacggta aaagggctga taattgtata
     1621 tttataatta tcagcccttt ttaatatata cgttatatcg gcaagcaaat caatatcatg
     1681 ttgacactcc ccgccatcaa tatttgcctg atttttagag cattattggg tctgtgcggt
     1741 ctatttttca tccccgtcaa ggagccctgt catataatcc tttgtatccc cgctcaacaa
     1801 atccaccaga taattgggct gcaccccgta tttttccctg taagcctgta tcgctgcaga
     1861 gacgacccaa tgaacctctt ctctcaattc tcttgcggac atcagggcag cgccttttcc
     1921 cctgatgatc atctgttcca ctgcatcgga tgtcgccacc gttatgcgat attttttgtg
     1981 atgatcgtgg acgaactttt ctatataatg atctgccgtc tgagcctccc tcgtgtatac
     2041 aatgtggata ttgttataat cgattatctc ctcccggcgt ccggggacac gataggcatc
     2101 gaatactagg ataatatggg tccttcggat cccctggtaa ttactcaaga tatcttgcag
     2161 cttttctcgc gccgcttcca tatgatcatc gtctaccaag gttttcaatt ccggccacgc
     2221 atggataata ttgtagccgt ccaccaaaag atattcttcc ttggactctg cccgctccat
     2281 tgtccgcacg ataggcctgt aattgctttc cttggtaggt tttcgtctct tccaggcaga
     2341 ctttttcccg cgattagcga agtaggtcct gtttattatt tcatcgactt cgtccggatc
     2401 aatccatgcc tccgccttgg acgaggcttt ggctacggct gcttcattta cgttgagtgg
     2461 atattcgcct ctatcctgaa agtagctttc gatgtgcatg tagtctggga ccctatccca
     2521 atcaaccaag aaccccacac cgtttgcaca gaataccgag cctgtggggt tatctatatc
     2581 cctttcggaa tcgtatccga tactctctat gacctcttcg ctgttatggc acggcttgta
     2641 tcctttaaag ctgcaaaaca gcctgccata cccccctgtg taggccatca cttccttgtg
     2701 ataatttttc atagcaacca ccggggcatc tccgctgagg gttacccttt caccatccga
     2761 atgcacaatt tgccacgatc cctgcatttg gtctatatcc gccatggctc gtcccaccat
     2821 cttttccgga agttctaaac gaaaactata atacggctcc aataataccg attctgcctg
     2881 ctttaacccc tggcggattg cacggtatgt agcctccctg aaatcaccac cttgggtatg
     2941 ctttttgtgg gccctgccgt ttaccagcgt aattttcata tccgtaatct ctgaacctgt
     3001 aagcacacct ttgtgtgact tctcctctaa atgagagagg ataaggtttt gccaacttct
     3061 acccaagaga tcttcgctac actgggatga aaatcttagg ccgctccccc tttttccggg
     3121 ctctaacagt aagtgaactt ccgcatagtg cctcaatggc tcaaagtggc ccactcccac
     3181 aacccgattg tcgatagtct ccttatagac aaccttgccc gcatcaaagg atacttcaac
     3241 accaaaacgg tcctttataa tatccccaag gatttctgtc tgcacttccc ccataatttg
     3301 aatctgtatt tccttaagct cctcatccca gactattcga agctcaggct cttcctcttc
     3361 aatcaatcgc agcttgggca gcatggccct tggatccaga ccctcaggaa gcaggatttg
     3421 gtaggataat acaggctcta ataagggtgc tttcgaatcc ctttctatcc ccagaccttc
     3481 cccgggccgc gtctggctaa gccccgtaac cgcacatatg gtgcccgctt ctgcccggtc
     3541 aaccatgtca aatttctccc ccgaataaat tcgtatctga ttgacctttt cctcccaaat
     3601 gccattggtt aaggggtctc ttatcttgat ttctccccct gtgattttca tgtaggtaag
     3661 tctgttatcc tgctcgtctc tggatatctt aaatacttta gccccaaagg ccttgggata
     3721 tgaaggttcg tcggcatact ctgcgatacc gtccataaac ctttggatac cctccacttt
     3781 gagggctgag ccaaaaaaac aggggaacac cttattcccc ttgataagcc ccgcgatttg
     3841 tgacgtcttt atgttgcctg tctccaaata ggattccata acggattcat cgcacatggc
     3901 aagatcttcg taaaagtctt ccgtaccgat accttcaaaa tcaatacaac cgtcgctcag
     3961 ggatcttttt aattccgcca tcagtttttc cttgtccgca ccttcctggt ccatcttatt
     4021 gatgaataga aaaaccggaa ttttatgcat ggcaagcaaa ttccacaggg tttcggtatg
     4081 tccctgtacg ccgtcggctc cgcttattac caatatggcg tagtctagta cctgaagggt
     4141 cctttccatt tccgcagaaa aatccacatg accgggggtg tccagaagcg ttatctctgt
     4201 cccgcccatt tcaaatacgg cctgttttga gaaaatggtg attcccctct ttctctccag
     4261 gccgtgtgtg tctagataag catccttgtt atccactctg cccaacttcc gggttttacc
     4321 gcttaaatag agcatgccct ccgataacgt tgtcttcccc gcatctacat gcgccagtat
     4381 ccccatgact aaccttttca tatactatct caaatccttc gcactcgctt tgagtacctt
     4441 ctattatgta tactagttta ccataagatg cagtatccaa caacgcctga ctagacccca
     4501 tggcgtgttg attaaaaccc ctggcaccga ttagtatatt ttcctgctat aatatatacc
     4561 caaagataca gaaagctaaa agtcaataac gcttttatgt cgctttctgg cttgacatgt
     4621 ataataatgc tttatataat gtatttgttg tatatatatg aataatgctg gaaattggta
     4681 atcattctta ggcctgcatc ataggcactg cagaagattg tgaattttag atacccggct
     4741 tatattctac tgcggattgt ttttgtccca aggtggggga atttctctat tttctattaa
     4801 tatctgaaat attggggagg gattgttatg ggcggaaata atttacatgg atctacggtt
     4861 ttgatgtttt tattctattt ggcttttatg atgatcgtcg gggtttattt ttacagaaag
     4921 agtaaagacg acgattccct atccggctac ctattaggtg gtcgaaactt aaatcctttg
     4981 gtggcagcca taagcgccgg ggcctccgat atgagcggtt ggcttttaat gggactgccc
     5041 ggtgcagcgt atcttgccgg tgtatcagcc ggttggatcg gcatcggtct tgcaattgga
     5101 acttggttca attggacttt tgtggccaag aggtttagga tttatactga agtatccggt
     5161 gatagcgtga cggtgtccga cttctttgaa aatcggttta gggataccag ccatatcctc
     5221 agggtagttt cagccttttt tatcgtattc tttttcctta tatatactgc atcgggtttt
     5281 gtagcgggcg cagtcctttt caacacggtc tttggtctga gctatattaa cggacttatt
     5341 ctaggcagcg ttgttatcct catctacact gttttaggtg gatttttagc tgtcagctgg
     5401 acagacctct ttcagggact attgatgata atcgccttgg taggggttcc cctgggagtt
     5461 atttcatcca taggcggacc ccaggtttcc ataatgtctg ccaaggccat caacccccat
     5521 gttctaaata tatttaaaaa tccggatggc acctccctag gattttgggc tacggcttcc
     5581 cttcttgctt ggggtcttgg gtatcccggc atgccccata tcataacccg ctttatggct
     5641 ataagagatg ccgatgaaac taaaaaggca agacatatag ctatattttg ggtgttctta
     5701 ggtttgttct gtgcggtctt aataggtgtt gtgggaatga cttacttaaa ggattccccc
     5761 ctccagggca cggatgtcga aaaagtcttt atgatattga tccaatccat ttttcatcct
     5821 gcgattgcag ggatattcct ggcagcagtc ctagctgcgg tcatgagtac tgttgactcc
     5881 cagttattgg ttgcctcttc gtcaatgacc aaggattttt atcaggtgtt tttaaaaaaa
     5941 gatgccggcg ataaggaatt ggtgatcttc gggcgaattg cagttgctgt tatctccatc
     6001 atcgcttttg taatagcttt aaatcccgac agcaaagtcc ttgatttggt ttcatatgca
     6061 tgggccggcc tcggagcaac ctttgcaccg attgtggtag tttccctcta ttggagggac
     6121 atgaacagac atggtgcctt cgcgggtatg ctggtagggg gtataacatc aatagtctgg
     6181 aacaaattga gcggtggcat ttttgatatc tatgagctac tccccggctt cctatttgca
     6241 tttatcgcta taattgtcgt cagcaaatta acgggtggtg cccagcaaga gattaaagat
     6301 gaatttgaca aggttgagtc catgctaatc aaaaaatcct gactttaaat agatgtgaca
     6361 gatggggacg ctccctgaca catggggacg tcccttattt gtcacatgcc ttttatttac
     6421 tacagcatat aattccgggg aaagtgtcca tctctcgggc gggagattaa ataccaggta
     6481 ggcaccgggc tttgcatatc gtcataccag gccaacactt ccttggactg attcatcata
     6541 gtgtcaatct cactttgtcc aaacggtata gctcagggta ccgcggtttt aaccgtttat
     6601 cacgatttaa accacgggcc taataatttg tccaatgttt tcgtacagcc aatttgccat
     6661 ttctatacaa cccttggcaa tttccggttg acccgatttc aaatagaagg ctgcaaagag
     6721 catctcgtga actactcatg actaaagtca cgagcttcta ggtaaaaacc cagaactttt
     6781 cctaattcac cgcaaactgc ataagttatt actaacccaa tgtctcacat ttgctccaat
     6841 agctaaaatt cttaactatg tctatctcta gactaggcta tccccgctgt cccagcggtt
     6901 ctgtcaatag ttatctaggc tgtttggcct aaacagttta atccttcgtt ccttatatta
     6961 acggatgcat taatgtccct gtctagcctt gtgccacagg aactgcatac ccattcccgt
     7021 tcgcttaggg ttaacattat attctt
//
LOCUS       DASRNX010000106        16524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_499339,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000106 DASRNX010000000
VERSION     DASRNX010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 16524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16524
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499339"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFD89_04135"
     CDS             complement(<1..306)
                     /locus_tag="VFD89_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HZJ57411.1"
                     /translation="MDMIKKEGKVWVINAGGYEAVIAPEIGGNVIALRKGDFDVLRHY
                     NSYGEVANAPVVYGLPILFPPNRIDGGKFEFSGRTYQFPIGEKRSNNSLHGFLSHRPW
                     "
     gene            727..1860
                     /locus_tag="VFD89_04140"
     CDS             727..1860
                     /locus_tag="VFD89_04140"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZJ57412.1"
                     /translation="MKQTNLIEYLDSLRGQGIPGSDCIVYKDHKEVFRHITGFADHKQ
                     TKPLTKNNTYWLYSASKVITCTAFMRLVDEGDVALDDPVSKYLPEYKEMMVKEGSGIR
                     KAEKVMTIRHLLTMESGLNYDLGAPSIKDVLKEKGNNATTRDIIRALANEPLDFEPGT
                     RYQYSLSHDVIGAVIEAVSGKGFGKYLYDHIFGPLGMENTGFKWTPKLKSNMSHQFMY
                     DETTMTSSPMTLNNPYIFSDNYESGGAGLISTVDDYILFLDAMCNGGVNKEGYRVLSM
                     ESIDEMRKDQLGNASKEDFDLHGKIGYSYGLGVRTLIDKEISGARSPLGEFGWDGAAG
                     AHALIDTDNRLAIFYAQHVHNCGFAYSVIHPTIRDLAYDMLGL"
     gene            complement(1900..2754)
                     /gene="fba"
                     /locus_tag="VFD89_04145"
     CDS             complement(1900..2754)
                     /gene="fba"
                     /locus_tag="VFD89_04145"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HZJ57413.1"
                     /translation="MALVNPMKMIEDAQKGGYAIAAFNVYNLETIQAVVEAAWEEGSP
                     LIIQTTPGSLKHAGIPYVAACVRTAAELYDIPICLHLDHCESFDTIVECIKAGYTSVM
                     IDGSRLLYNENVAIVRKVVDIAHRAGVAVEGEIGRIGGTEDDMTTDDGDAGLTIPEEA
                     RDFAEATGVDILAVAIGTAHGLYRGEPKLDFDRLSCINRLVGTPLVLHGGSGVPDESV
                     QESIKRGICKVNIATELKVPMANTIQGVFRDNPKENDPRKYMGAAKEAVKQVARKKIR
                     LCGSQQKG"
     gene            3148..3723
                     /locus_tag="VFD89_04150"
     CDS             3148..3723
                     /locus_tag="VFD89_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57414.1"
                     /translation="MYFTKSLSRNLRILHKITKSWLAAKKIALTAILSALSAILQSAG
                     GLLPGVGLLISPLATLPILLSTVISLKHGICAYLLTNILLVLIEPSELHIFAFTTGLL
                     GLATGWGLRSLDRRAIIIPINGVVLSAGICLPLYILGFPILGPALSTSPDIKSIIIIF
                     LFSVFYNWLWLELGLYILSIFSKFLSSETKS"
     gene            complement(3720..3965)
                     /locus_tag="VFD89_04155"
     CDS             complement(3720..3965)
                     /locus_tag="VFD89_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57415.1"
                     /translation="MKELLNIITADVFRCILNDKNLKVCQLNSIINLLIKEEIQFTLT
                     FTPMTPTAVANAALAIQIRPDISLSIIVSFDSGTILL"
     gene            complement(4152..5492)
                     /locus_tag="VFD89_04160"
     CDS             complement(4152..5492)
                     /locus_tag="VFD89_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57416.1"
                     /translation="MLNGNYTEAQMMTQERRLDPKDIALPPGYEIEVFAKGLTTPINI
                     TYTETGDMLVADSGVADGNGKVMKLTDKGFVVIADGFNPPLTGINVYNGNIYVSHRRF
                     VTMVLPDGSKKDIIDGLPSNGDHHNNRVVFGPDGKMYFGQGTATNTGVPGKDNDWVPE
                     HPFFHDRPGGTITLSGENFRSSTFLEDFPERQTVTGAYEPFGVPSYPGKVIDGITRAN
                     GSIMRANPDGSDLELVVWGLRNPFRIRFDRYNRLFAANHGMDVRGSRPVANSPDEFQW
                     IQPGCWYGWPDYTGGLPVTLPQFKPEGEPQPRFLWAKHPMHPPRPFTTFQPHSAVMGF
                     SFNDNPYFAPVGDAYIAEFGAIIPTNTGGRPLFNVGHRISRIDMNNGNVSTFARNHSG
                     LPAHLTNGGGFERPIDAVFGPDGMLYVSDFGIYTATGPVKGTGVIWRISRKGAV"
     gene            5575..5880
                     /locus_tag="VFD89_04165"
     CDS             5575..5880
                     /locus_tag="VFD89_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57417.1"
                     /translation="MGFGQDLLRLINFIRFPSEMHLWEGLLLMENTRGIFFSSDLMFR
                     LGKADNMVIEGSWDYEVRNITPTQVPSSEARTALQNILAGLTPKFVATGHGPCIKVL"
     gene            complement(5987..6952)
                     /locus_tag="VFD89_04170"
     CDS             complement(5987..6952)
                     /locus_tag="VFD89_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019381784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZJ57418.1"
                     /translation="MGKKHIIGIVLILAALIIGFASPVSNFGMALSGVKPHKKVAPES
                     KDENTELYEEIRKFGEENKTDPMDATVDRVWKAIPGYNGLIVDIEASYEKMKPDGKFD
                     EGKIVYREVPPEVRLKDLGPEPIYRGNPQKPMVALLINVAWGNEYIPQMLKTLENHKI
                     KATFFFDGSWVNNNPDLAVEIKEAGHEIGNHAYSHPDLSGYSANQTIDELEKTNRVIM
                     ETLDVKPEWFAPPSGSFNADTVRVAHELGMRTILWTVDTVDWRKPDTWEMVNRVVSKV
                     ENGSMILMHPTKPTAEGLDTMIKNIKAKGLRLGTVSNLMSEGRLD"
     gene            7184..7387
                     /locus_tag="VFD89_04175"
                     /pseudo
     CDS             7184..7387
                     /locus_tag="VFD89_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            7484..7801
                     /locus_tag="VFD89_04180"
     CDS             7484..7801
                     /locus_tag="VFD89_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZJ57419.1"
                     /translation="MVQKTNLSKELSTGILAPSVDTDNIKIGEAKVFDVDGKRRGAFR
                     DKDGRVHLVDLVCTHMGCEPQWNTAEQSWDCPCHGSRFTYDGQVLEGPAKKPLANAEI
                     IDS"
     gene            complement(8076..8468)
                     /gene="rpsI"
                     /locus_tag="VFD89_04185"
     CDS             complement(8076..8468)
                     /gene="rpsI"
                     /locus_tag="VFD89_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZJ57420.1"
                     /translation="MAKVQYFGTGRRKKSVARVRLVPGDGNIVINSREIDNYFGLETL
                     KAIVRQPLELTETLGRFDIIANVHGGGFTGQAGAIRHGISRALLKADEELKPALKKAG
                     FLTRDPRMKERKKYGLKGARRAPQFSKR"
     gene            complement(8491..8919)
                     /gene="rplM"
                     /locus_tag="VFD89_04190"
     CDS             complement(8491..8919)
                     /gene="rplM"
                     /locus_tag="VFD89_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZJ57421.1"
                     /translation="MRTFMAKAQEVERNWYVIDADGKTLGRLASEVAKILRGKNKPIY
                     TPHVDTGDHVIIVNAEKIHLTGKKLDQKLYRRHSQYPGGLKEIPYRQLMQTRPTLAIH
                     EAVKGMLPKNSLGRQMIKKLRVYAGPEHDHQAQMPVELDI"
     gene            complement(9052..9564)
                     /locus_tag="VFD89_04195"
     CDS             complement(9052..9564)
                     /locus_tag="VFD89_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HZJ57422.1"
                     /translation="MKIFKHKGFTVVWTILRVWLGYNWLMSGIEKLRDPAWVGSEAGT
                     AISGFFQGSIANSAGQYATVPSWYAGFIERFAMPFSKIFTYLIPIGETLVGISLILGA
                     LTLLGLLGSALMNFNYLLAGAGGINTMMYTVAILLLVAGSAAYYIGLDKVLVFPILRR
                     FFKFMPEEGQ"
     gene            complement(9949..10710)
                     /gene="truA"
                     /locus_tag="VFD89_04200"
     CDS             complement(9949..10710)
                     /gene="truA"
                     /locus_tag="VFD89_04200"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZJ57423.1"
                     /translation="MKRNIKLTIEYDGTNYGGWQRQVNSPTIQQELEEGLFKLTGEDI
                     GIQGAGRTDGGVHARGQVANFFTKSSIAPQRFGFALNSVIPPDIRIVRSEEVESDFHS
                     RFCAKARHYRYSMVTGPQGIAIGRDYYYHVPVPLDMGEMEKAIVMFKGTQDFKAFQAS
                     GSIARTTVRTIYDARLTFQDPFLYLDILGDGFLYNMVRIIAGTLLYVGKGKIDYNNIV
                     EIIKMGDRRLAGPTAPPHGLCLQKVYYPGDDIVLS"
     gene            complement(10712..11524)
                     /locus_tag="VFD89_04205"
     CDS             complement(10712..11524)
                     /locus_tag="VFD89_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HZJ57424.1"
                     /translation="MLKDITIGQYFPGRSFVHRLDPRIKIVISFLFIVLIFFVKTFLG
                     YGFVLLFLAAVVAVSGIPVKYVIKGLKPLAFIILLTFFLNVFFTADGTEIWRLGFLVL
                     TLKGLRQGVFMALRLVFLVSGTSLLTLTTSPISLTDGIESLLRPLKAIRFPSHELAMM
                     MTIALRFIPTLLEETDKIMKAQMARGADFESGNILQRAKALVPLLVPLFIGAFRRADE
                     LAMAMESRCYRGGDNRTRMKVLKVDRGDYIALSATLVFALLIIWDFAGRGGL"
     gene            complement(11517..12380)
                     /locus_tag="VFD89_04210"
     CDS             complement(11517..12380)
                     /locus_tag="VFD89_04210"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HZJ57425.1"
                     /translation="MSIIIDNLTHVYMEGGPFESAALDGVNLRIEDGEFIGLIGHTGS
                     GKSTLVQHLNGLLKPTSGIIRIDDITVENKSKRLKELRQKVGLVFQYPEHQLFEERVF
                     SDIAFGPKNLGCSGHEIEQRVKESMELVGLDYDSMKDRSPFELSGGQRRRVAIAGVLA
                     MKPKILILDEPTAGLDPRGRDEILGRVAQLHKKYDLTIILVSHSMEDVAKLVDRIIVM
                     NKGKIALDGSPGEIFKNSQRLDDMGLAVPNITRLMKMLRASGMDVPEDIFTVAKAKEA
                     VLKALRRDSDA"
     gene            complement(12371..13207)
                     /locus_tag="VFD89_04215"
     CDS             complement(12371..13207)
                     /locus_tag="VFD89_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HZJ57426.1"
                     /translation="MIKSIIEASNVGFSYINYEEEEIAALKDVSLDVKKGEFIAVIGH
                     NGSGKSTLAKHINALIKPDRGIILIKGMDTSDDEMVWDIRQTAGMVFQNPDNQLVATI
                     VEEDVAFGPENLGYPPEEIRARVDDSLEKVGMEGFAEFAPHLLSGGQKQRIAIAGIIA
                     MRPEIIILDEPTAMLDPSGRREVMETIHYLNREEGITIIHITHYMEEAIDAHRVLVME
                     EGQVVLEGSPKEIFIKVEELKSLGLDVPQITELAFELRKEGIDIPPGLLTAEEMVKAL
                     CQ"
     gene            complement(13475..13987)
                     /locus_tag="VFD89_04220"
     CDS             complement(13475..13987)
                     /locus_tag="VFD89_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HZJ57427.1"
                     /translation="MPGHRKLSRPSDQRKALLRNQVTELLWHGRIKTTGARAKEVRSI
                     AEKMITLAIKEYDKTIKVTKEMNNERGQTVTKEVVNDLPSRLNARRKMMAYLYDYQEI
                     RGKDENKEDYRERTKDVNHPLIEKMFSEYGPKYRKRNEELNSGGGYTRILKLGPRRGD
                     GAEEVIIELV"
     gene            complement(14050..14997)
                     /locus_tag="VFD89_04225"
     CDS             complement(14050..14997)
                     /locus_tag="VFD89_04225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZJ57428.1"
                     /translation="MIEIEKPKIELIELEEDGTYGKFVVEPLERGFGTTLGNSLRRVL
                     LSSLPGAAITSIKVEGVLHEFSTIPGIKEDLVEIILNLKELVIKMEQDEAKVVTVNIQ
                     GPGELTGADILTDVGVEILNGDKHIATLNEDARLYMEIAIERGRGYVPAERNKKSGQP
                     IGLIPIDSIFTPIRRVNFHVEDTRVGQVTDFDKLTLEVWTNGSLKADEAASLAAKIMN
                     EHLMLFIDLTEHVNEVEIMVEKEEDKKEKVLEMTIEELDLSVRSYNCLKRAGINTVDE
                     LIQKTAEDMMKVRNLGKKSLEEVEQKLAVLDLSLKKSDE"
     gene            complement(15067..15693)
                     /gene="rpsD"
                     /locus_tag="VFD89_04230"
     CDS             complement(15067..15693)
                     /gene="rpsD"
                     /locus_tag="VFD89_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZJ57429.1"
                     /translation="MAKYTGSVCRLCRREGIKLYLKGDRCYSDKCAISRRPTPPGQHG
                     QRRKKASEYGIQLREKQKARRIYGVLEKQFRRYYGQAERMRGITGENLLQLLEKRLDN
                     VVYRLGFAESRAQARQFVLHGHISVNGKRVDIPSYLTDVGEIIAVRQKAATEIEHFKA
                     LREGAGKTVVPWLQVDYEKLEGTVSALPAREDIDVPIQEHLIVELYSR"
     gene            complement(15709..16104)
                     /gene="rpsK"
                     /locus_tag="VFD89_04235"
     CDS             complement(15709..16104)
                     /gene="rpsK"
                     /locus_tag="VFD89_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZJ57430.1"
                     /translation="MARKQVRRTRRRRERKNIERGAAHIRSTFNNTIVTITDTAGNAL
                     SWASSGGLGFRGSRKSTPFAAQMAAETAAKAAMEHGLRTVEVFVKGPGAGREAAIRSL
                     QATGLEVSMIKDVTPIPHNGCRPPKRRRV"
     gene            complement(16117..16488)
                     /gene="rpsM"
                     /locus_tag="VFD89_04240"
     CDS             complement(16117..16488)
                     /gene="rpsM"
                     /locus_tag="VFD89_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZJ57431.1"
                     /translation="MARIAGIDLPRGKRVEVGLTYIYGIGRSKSNEILAATDINPDTR
                     IRDLSEVEVNRLRDYIDRNVTVEGDARREVSLSIKRLGEIGCYRGIRHRRGLPVRGQS
                     TKQNARTRKGPKRTIGIRRNK"
BASE COUNT         4069 a         4294 c         3391 g         4770 t
ORIGIN      
        1 ccaggggcga tgggacaaaa agccatgcag cgaattgttg ctacgtttct ccccgatagg
       61 gaattgatac gtcctccccg aaaattcgaa tttgccccca tcgattctgt tggggggaaa
      121 caatatggga agtccataga ctaccggtgc attggcaacc tccccatagg aattataatg
      181 tcttagtaca tcgaagtctc cctttcgcag tgcgatgaca tttcctccaa tttccggtgc
      241 gataactgct tcatacccgc cggcattgat cacccaaacc ttgccttcct tttttatcat
      301 atccatgctg tcctctcctc acttaagtat tgtttttatc ccgtgtcgtc cgtataaaac
      361 ggggtatctg tatccttaat tgggttctct taaatcatct tatattatgc tctatattgt
      421 ataccgtgaa ataagtccca ctatttccat gcctcggagt acgttgtctc gaggcgtgaa
      481 taaaaggttt attgggtttt gatacggata aacaataggt caacgaaatt tatctaaaga
      541 tgataaattt gtagttagga ttgtttatcc atatggtggg cctttacgta acatattccg
      601 taacatatat agattataca ataatcgccc agtgaaatcc atcatataat attaattcat
      661 ggggattcca ataatggagt caatgtagta aaatacccat ataacataaa aaaggatgtg
      721 taaaagatga aacagactaa tcttatagag tatctcgact ccctccgggg ccaaggcatc
      781 cccgggagcg attgcatagt ctacaaggac cataaggaag tatttcgcca tattacgggt
      841 tttgccgatc acaaacagac aaagcccctg acaaaaaata atacatattg gctctactca
      901 gcctcaaagg tcatcacctg cacggccttt atgcgactgg tggatgaggg agacgttgct
      961 cttgatgatc ccgtatcgaa atatctccct gaatataagg aaatgatggt caaggaaggt
     1021 tccggcatcc gaaaagcgga gaaagttatg acaataaggc atcttttgac catggaaagc
     1081 ggcctcaatt atgatctagg ggcaccatcc ataaaggatg tcctaaagga aaagggtaac
     1141 aacgctacta ccagggacat aataagggcc cttgccaatg agcccctgga ttttgaaccg
     1201 ggaacccgtt accagtatag cctgagccac gacgttattg gagcggtgat agaggcagta
     1261 tccggcaagg ggtttggtaa gtacctgtat gatcatatct ttgggccgct gggcatggaa
     1321 aataccggat ttaagtggac cccaaaacta aagtcaaata tgtcgcatca gtttatgtat
     1381 gatgagacca ctatgacctc atcgcccatg acattaaaca atccttatat cttctcggat
     1441 aattatgaaa gcggcggtgc cggccttata tcaacggtag acgattatat tctattctta
     1501 gatgccatgt gtaacggcgg ggtaaataag gagggttaca gggtcttgtc catggaatcc
     1561 atagatgaga tgcgaaagga tcagctgggc aatgcctcca aggaggattt tgatctccat
     1621 ggaaagatcg gctacagcta tgggcttggc gttcgcactt taattgacaa ggagatatct
     1681 ggggccagga gcccgttagg tgagtttgga tgggacggcg ctgcgggagc ccatgctcta
     1741 atcgacacgg ataaccgcct tgccatcttc tacgcccaac acgtccataa ttgtggtttc
     1801 gcctacagcg ttatacatcc aaccatcagg gacttggcat acgatatgtt agggttatag
     1861 aacacaaggg gacggttcca ctgtgtttgg gtccctattt catccctttt gttgggaacc
     1921 gcacaggcgt atctttttgc gggctacctg tttgacggcc tccttcgcgg cccccatata
     1981 tttcctcgga tcgttttcct tgggattatc ccgaaatacc ccttggatag tgtttgccat
     2041 gggcaccttg agttcggttg caatgtttac cttgcatatc ccccgcttaa tggattcttg
     2101 tactgactcg tctggtactc cggaaccccc gtggagcacc aggggcgttc ccaccaatcg
     2161 attgatacaa gagagcctgt caaagtccaa ttttggttct cccctgtaaa ggccgtgggc
     2221 agtccctatt gcgacggcca agatatcgac ccctgtggcc tcggcaaaat cccgagcttc
     2281 ctccggtatg gttagtcctg catccccatc atccgtggtc atatcgtcct cagttccgcc
     2341 gatccttccg atttccccct ctactgcaac ccccgcccta tgggcaatat caacaacctt
     2401 ccttacgata gcaacgtttt cgttatagag cagcctggaa ccgtctatca taacggaggt
     2461 ataccctgcc tttatgcact ccactatggt atcaaaggat tcacagtggt ctaaatgcag
     2521 acagatgggt atatcgtata gctctgcagc tgtcctcaca caagccgcaa cgtagggaat
     2581 tccggcatgc tttaaggagc cgggtgttgt ttgaatgata aggggagagc cctcctccca
     2641 cgcagcctca accacagcct gtatggtctc taagttgtac acattaaagg ccgcaatagc
     2701 atacccccct ttttgtgcat cctctatcat cttcatggga ttgaccaatg ccatttgtaa
     2761 agcccccttc tataattgct tattgatgtt ttttccaaac cgtccccata tcacaccgta
     2821 ttgcccttaa tcaggaaata tgctcaagtt gtttactgca attgcatgaa caacttttgg
     2881 tagccattgc cggtatatgc taaacgccat atgagatctt ttttgaatag cctagcagga
     2941 caagccccct ataccgtata tttcaccccc ttgtcccggg tgttgttcta ctttaattat
     3001 acaccacttt atgccaaaga acgagtgccc catggattca ttcaattctt tattgattca
     3061 tcccatgtat gaattcacga gtcttcccgg catatctata atagtataaa gcatttgttt
     3121 tcaatatcta aaaaatgggg tgtagacatg tattttacca aatcattgtc acgaaacctt
     3181 cggatcctgc ataagattac aaaatcctgg ctcgctgcaa aaaagatagc ccttaccgcc
     3241 atattatcgg cgctttctgc tatcttacaa tcggcgggcg ggttattgcc aggggttgga
     3301 ctgcttatca gcccccttgc aaccctgccg attcttctta gcactgtcat ctcattaaag
     3361 cacggcatat gcgcatacct tttaacgaat atcctgttgg tcctgataga acccagcgaa
     3421 ctgcatatat tcgcctttac gactgggcta ttagggctgg caaccgggtg ggggcttcgt
     3481 tcattagatc ggcgggctat cattatcccg ataaacgggg ttgtcctttc tgccggcata
     3541 tgcctgccgc tttacatatt gggatttccc atcctgggcc ctgcgctttc tacgtcaccc
     3601 gatataaaat ccataatcat tatctttttg ttttctgtat tttataattg gctgtggttg
     3661 gaactgggtc tgtatattct cagcatattt tccaaattct tgtcctcgga aacaaaatct
     3721 taaagcagaa tcgtccccga atcgaagctt acgataatgg acagggagat atccggtcga
     3781 atttggatgg caagagcagc atttgcgaca gccgttggcg tcataggcgt aaaggtcaag
     3841 gtaaactgga tctcctcttt gattagcagg ttgatgatgg aatttaattg gcataccttc
     3901 aggtttttgt cgtttagaat acaccgaaaa acgtctgcag ttattatatt caacaattct
     3961 ttcaaaacag ttacccccag taacagatta ttgacccaac tcccgcaagg attaaaaaaa
     4021 tacctcggta attttggcta aagtgaacgg aattaccgag gtgtctttat gcggctaata
     4081 aattgtccga cttgttaagt acctatggtt tcggcgatac aatacttgcg cctatataca
     4141 cccggaattg tttaaactgc accctttcgt gatatcctcc agattacgcc ggtgcccttg
     4201 acaggacccg tagcggtata gatgccaaaa tccgaaacat acagcatacc gtcggggccg
     4261 aatacggcat caatgggtcg ttcaaatcct ccgccgttag ttaaatgtgc cggaaggccg
     4321 gaatgattcc tcgcaaaggt gcttacattt ccattattca tgtcgatccg agagatgcga
     4381 tgaccgacgt tgaacagggg ccttccgcct gtatttgtcg ggattatagc gccgaactct
     4441 gcaatgtagg catcccccac cggtgcgaag tatggattgt cgttaaagct gaatcccatt
     4501 actgccgagt gcggttgaaa tgtagtaaac ggtcgcggcg ggtgcatggg gtgtttcgcc
     4561 cataggaatc ttggttgcgg ctcaccctcg ggcttgaatt gtggcagtgt aaccggcagg
     4621 ccgcctgtat agtcgggcca accgtaccag catccgggtt gtatccattg gaattcgtcc
     4681 ggtgaattcg caacgggcct gcttccccga acatccatac cgtgattggc tgcaaataag
     4741 cgattatatc gatcaaagcg tatcctaaag gggtttcgga gaccccacac caccaattcc
     4801 aggtccgagc cgtccggatt ggccctcatt atgctgccgt ttgcacgggt tatcccgtct
     4861 atgaccttac ccggatatga cggtacgcca aagggttcat aggcgcctgt taccgtctgc
     4921 ctttccggaa agtcctccag gaacgttgaa cttcggaagt tctctccact taaggtaatg
     4981 gtgcccccgg ggcggtcatg gaaaaatggg tgttccggca cccaatcgtt gtcttttcca
     5041 gggaccccag tgttggttgc ggttccctgg ccaaaataca tcttcccatc gggcccaaaa
     5101 acaaccctgt tattgtgatg gtccccgtta ctcggaagcc cgtcgattat atccttcttt
     5161 gaaccgtcgg gcaacaccat cgtaacaaaa cggcgatgtg acacatagat attcccgtta
     5221 tacacgttaa tgcctgtaag cggggggttg aacccatcag cgattactac aaagccctta
     5281 tccgttaatt tcatcacctt cccgtttccg tcggcgacac cggaatctgc aacaagcata
     5341 tccccggtct ccgtataggt gatgtttata ggagttgtca gaccctttgc aaaaacctcg
     5401 atctcatagc cgggcggaag cgcaatatcc tttggatcca gtcttctctc ctgggtcatc
     5461 atctgcgcct cggtatagtt tccattaagc atcctaaagc ctctcctttc caatatcttt
     5521 tatcatttta ttctgcatcc gcccccttcg tgactcattt aacaggatgc cgggatgggc
     5581 tttggacagg atcttttgcg cttaataaat tttattagat tcccttccga gatgcacctg
     5641 tgggaagggc ttttgcttat ggagaatacg aggggcatat tctttagcag cgacttgatg
     5701 tttcgcctgg gaaaggccga taatatggta atcgagggct cttgggatta tgaagtacgc
     5761 aatataaccc cgacccaggt gcctagctct gaggcaagaa cagcccttca gaacattttg
     5821 gcagggctaa cccctaaatt cgtagccacc ggacacgggc cgtgtataaa agttttgtaa
     5881 gggacattgc cattgtagat aaagggacgt ttaatatgcc tacagaaaaa gccggcggca
     5941 tatcataaca tgctaccggc ttttagatac tatatcctat tgataactaa tctagcctgc
     6001 cctcgctcat gaggttactg acggtgccca atcgaagtcc cttggccttg atgttcttta
     6061 tcatggtgtc cagaccttcg gcagtaggct tggtaggatg catgaggatc atggaaccat
     6121 tttcaacctt actgacaacc ctgttgacca tttcccaggt atcaggcttc ctccagtcaa
     6181 cggtatccac agtccagaga atggttctca tacccagttc atgggctacc cgaactgtat
     6241 ccgcattaaa gctcccactt gggggcgcga accattccgg ctttacgtca agggtttcca
     6301 ttatgaccct gttggtcttc tccaactcgt cgatggtctg gttcgcggaa tacccgctga
     6361 ggtcgggatg gctgtatgca tggtttccga tctcgtgtcc tgcctctttt atctcaactg
     6421 ccaggtcggg attattattg acccagctcc cgtcaaagaa aaatgtcgct tttatcttat
     6481 ggttttccag tgtcttgagc atttgtggga tatattcgtt tccccatgcc acgttaatca
     6541 gcagcgctac catgggtttt tggggattgc ccctgtagat gggttccggt cccaaatcct
     6601 tcaagcggac ctcgggcggt acctccctgt atacgatctt gccttcatca aatttcccat
     6661 ccggtttcat cttttcataa ctagcttcaa tatcaacaat cagtccgttg tacccaggaa
     6721 ttgctttcca taccctgtct accgtcgcat ccatggggtc tgtcttgttt tcttcaccaa
     6781 acttccgaat ttcttcataa agctcggtgt tttcgtcctt agactcaggc gcaacctttt
     6841 tatgcggctt aactcccgaa agggccatgc cgaaattact cacggggctg gcaaagccta
     6901 ttatcaatgc cgctagaatc aaaactatgc cgattatatg cttctttccc attaccaccc
     6961 tccttccttt tttaatatta cctggatctt atgcctataa acataaacga aatctaaaag
     7021 atatactatt atggttcccg aagtgctgta tgctatcctt tcttctacat cgcaagtgaa
     7081 tattttatca ttgactcgga aaaattaaaa cgacaggaaa agttgcgcac cattctgatg
     7141 tgctgtttta gcgggtgagc accacaatac cggacaaggc ggcgatacca acatacatta
     7201 tagtaatctc cttgatacgg ccaacaccgt atttgatgcc aatgaggtat tgtaccactg
     7261 gtccgcccag gactacaaca cattggatca agtcccttat ataggtcagc tatccaccaa
     7321 cacccccggc atctatgtcg caacgggatt taaaaaatgg ggtataagca catctgtggc
     7381 agcagccctt ctgctgacga actctataac cggaaaggct aacccatggg cgccggtatt
     7441 cgatcctacc cgatttaaac ccgatgcctc gactgaaaat gttatggtcc aaaagaccaa
     7501 cctatcaaag gaactatcca ctgggatcct cgccccatcg gtagatacgg ataatattaa
     7561 aataggggaa gcaaaggtct ttgatgttga cggtaaaaga agaggtgcat ttagggataa
     7621 ggatggacgc gtgcatctag tagatctcgt ctgcacccat atgggctgtg agccccaatg
     7681 gaacactgca gaacaatcct gggactgccc ctgccatggt tcccgcttca cctatgacgg
     7741 gcaggtactg gagggccccg ctaaaaagcc actagcaaat gcggagatta ttgacagcta
     7801 aacttgatcc aaaagagcag tctaaatgga ctgctctttt ggattctctt tattctattt
     7861 cgaattaatt ctttttgtgc cgcctgtgct gagtattccc catcctgcat agagttgtct
     7921 ttccacttaa taatgaaaac aaaacgaatt tattagattc ccatcccgcc ggctattgaa
     7981 tacattcttt taagtattcg gctcctaggt gatccgcaca aaaataaaag agccgcattt
     8041 ctgcggctcc gtcatacata aattttttaa tataattatc tcttggagaa ctgtggagcc
     8101 cttcttgcac ctttgagccc gtatttcttt ctttctttca ttctggggtc gcgagtcaag
     8161 aagccggcct ttttgagagc aggtttcagt tcttcatcag ccttgagcag tgccctggaa
     8221 ataccatggc gaatcgcccc ggcctgtcct gtaaacccgc cgccgtgtac attggctatt
     8281 atatcaaatc tcccaagggt ctccgtcagt tctaaaggtt gacgtacgat tgccttcaga
     8341 gtttccagtc caaaataatt atcgatttct ctgctgttaa taacgatatt cccatccccg
     8401 ggaaccaatc taaccctagc aacggatttc tttcttcttc ccgtaccaaa atactgtact
     8461 tttgccaatc ccgtttcctc ctctcgtccc ttatatgtcc agctctactg gcatttgtgc
     8521 ttgatggtca tgttccggtc ctgcatagac acgaagtttt tttatcatct gcctacctaa
     8581 agagttcttg ggtagcattc cctttactgc ttcatgtatt gccagggtag gccttgtttg
     8641 catcagctga cgataaggaa tctcctttag tcctccagga tattggctat gcctgcgata
     8701 taatttctgg tctaatttct ttccagttaa atgaattttc tcagcattta ctataatcac
     8761 atgatccccg gtatctacat ggggagtata tatcggttta ttctttccac gcaaaatttt
     8821 ggcgacctca gaggccaatc ttccgagggt tttaccatcg gcatcaatta cgtaccaatt
     8881 acgctccact tcttgcgcct ttgccatgaa ggtcctcatt gctttccctc ctaattgaaa
     8941 tcagatgtat ctcaggatcc ggggctagtg gatcgtaatt tacaccacac tctattttaa
     9001 tacatgccac tatcagtgtc aaggaaaaat ttatctgcaa aggcctaaat ttcactggcc
     9061 ttcttcgggc ataaacttaa aaaatctcct cagtatggga aacaccaaaa ccttgtccag
     9121 gccaatatag taagcggctg acccagccac taaaagcaat atggcaacgg tgtacatcat
     9181 ggtgttaatt ccccccgctc cggctaacaa atagttaaag ttcatcagtg cactgcccag
     9241 caatcctaat agggtcaatg cgcctaaaat aaggcttatc cctacgaggg tttccccaat
     9301 gggtataaga taggtaaata tctttgaaaa gggcatagca aacctttcga taaaccctgc
     9361 ataccagctg ggcaccgtgg catattggcc agcagagttt gctatgctac cctgaaagaa
     9421 accggaaata gccgtgccag cctcgctccc tacccaggcc ggatctctca gcttttcaat
     9481 cccagacata agccagttat atcccaacca taccctcaga atcgtccata caactgtaaa
     9541 tcccttatgc ttaaatattt tcatataaat acctcgctac tcttatttag tttaatccat
     9601 tacactatct acagataagc aatatgaaaa ctaaattaat cggcatgaca attgcgatag
     9661 ttctatgcaa tttcataaat attaatttta caaatggttg tcacataaca tatagtttat
     9721 tatcccattg catcgtagag gtaatgaatt attatcaagc ggatgacttc accgatacag
     9781 catccaatag ccattgccaa tttatttaac tgacacaaga catcagcttt accgctttcc
     9841 tatatataac taaataagtc aactttaaaa cacggtatcg tgcgtctata gtaattattt
     9901 ttcacctttt atggattttt attccgatgt tcccctgtag cgaggctatt aagacaagac
     9961 gatatcatcc cccgggtaat ataccttttg gaggcatagc ccgtggggcg gggcggtagg
    10021 ccctgccagc ctcctatccc ccatctttat aatctccact atattgttat aatctatctt
    10081 ccccttgccc acatataaaa gggtgcccgc aataatcctt accatattgt acaagaatcc
    10141 atcgcctaga atatccaagt agaggaaggg gtcctgaaag gtgagccggg catcgtatat
    10201 ggtcctcacc gtagttcttg caatgctacc ggaagcctga aaggccttaa aatcctgggt
    10261 acccttgaac ataactatgg ccttttccat ttcccccata tccaagggta ccggcacatg
    10321 ataataataa tccctgccta tggctatccc ctggggaccc gttaccatgg aatacctata
    10381 atgcctggcc ttggcacaaa accttgaatg aaaatcggac tctacctctt ccgaacggac
    10441 tatccgaatg tccggtggga tcacagaatt taatgcaaag ccgaaccttt ggggcgcaat
    10501 ggaggattta gtgaaaaagt tggctacctg gcccctggcg tgtacgccgc catcggtcct
    10561 ccccgccccc tgaatcccaa tgtcctcgcc ggtcaattta aaaagccctt cctccagctc
    10621 ttgctgtata gtcgggctgt tgacctgacg ttgccaacct ccgtagttgg tcccgtcgta
    10681 ttcaatggtc agttttatat ttcttttcat tctacaatcc cccacgtccg gcgaagtccc
    10741 atattatgag aagcgcaaac accagggtag cggaaagggc aatataatcc cccctgtcca
    10801 ccttcaagac cttcatacgg gtgcggttgt ccccgcccct gtagcacctg gattccatgg
    10861 ccatagccag ttcgtctgcc ctgcgaaaag cccctataaa caaggggacc aacaggggta
    10921 ctaaggcctt ggccctttgc aggatattgc cgctttcaaa gtctgctccc ctagccatct
    10981 gagccttcat tatcttatcc gtctcttcca ataaggtggg aataaacctt aaggcaatgg
    11041 tcatcatcat ggccaattcg tgggagggaa acctgatggc cttgaggggc cttaggagac
    11101 tctctatccc atctgtcagc gaaatcggcg aggtggtaag ggtcaaaagt gatgttcccg
    11161 agaccagaaa tacgagcctt agggccatga aaaccccctg cctcagtcct ttcagggtca
    11221 ataccaaaaa acccaacctc catatttcgg tgccgtctgc ggtaaagaaa acgttcagaa
    11281 aaaaggtaag taaaattata aatgccaggg gttttaaccc ttttatcaca tatttaacgg
    11341 gtatgccgga aacagcaact acggctgcta aaaataacag tacaaagcca taccctaaaa
    11401 aggtcttgac aaagaatatt aaaactataa acaaaaagct tatgactatt ttaatcctgg
    11461 gatccagcct atggacgaag gatcttccgg gaaagtattg gccgatggtt atatccttaa
    11521 gcatcagaat ccctccttaa ggccttaagt acagcctcct tggcctttgc tacggtgaat
    11581 atatcctcgg gaacgtccat acccgaggcc ctgagcatct tcatgagtct agttatgttg
    11641 ggtactgcca gtcccatgtc atcaagcctt tgggagtttt tgaagatctc cccggggcta
    11701 ccgtccagtg caatcttccc cttattcata actattatcc tgtccaccaa cttggctaca
    11761 tcctccatgc tatgggatac cagtataatt gttagatcat actttttgtg tagctgggct
    11821 accctgccta aaatctcgtc cctgcctctg gggtccaggc ccgcagtcgg ctcatccagt
    11881 ataagtatct tgggtttcat agccaatacg ccggctattg caacccttct cctttgcccg
    11941 ccgctaagct caaaggggga tctgtccttc atagaatcat aatctagccc taccagttcc
    12001 atggactctt ttaccctttg ctctatttca tgccccgagc agcccaggtt cttaggcccg
    12061 aaggcgatat ctgaaaagac cctctcttca aaaagctgat gctccgggta ttgaaatacc
    12121 aggcccacct tttgccgtag ctcctttaga cgcttcgatt tattttccac cgtaatatca
    12181 tcaatcctta ttatcccgct ggtgggtttt aaaagtccgt ttaagtgctg gacaagggtc
    12241 gattttcccg agcccgtatg cccaataagt cctatgaatt caccgtcttc gattcttaag
    12301 tttaccccat ccaaggcggc agattcaaat ggaccgcctt ccatataaac atgggtcaag
    12361 ttatctatta ttattgacat agcgccttca ccatctcttc cgccgtcaat agacccggag
    12421 gtatgtctat tccttccttt ctaagctcga aggccaattc tgttatctgg gggacatcca
    12481 accccaaact cttaagctcc tcaaccttta taaagatctc cttggggctt ccctccagca
    12541 ctacctggcc ctcttccatc accaaaaccc tgtgggcatc tattgcctct tccatatagt
    12601 gggtaatatg gattatggtt attccttcct cgcggttgag ataatgaatg gtctccatta
    12661 cctcccgcct tccggaaggg tccaacatag ccgttggttc gtctaaaatt attatctccg
    12721 gcctcatggc tataattcca gcgatggcta tcctctgttt ctggccccct gaaaggaggt
    12781 gaggcgcaaa ttctgcaaag ccttccatgc ccaccttttc aagggaatcg tctacccgcg
    12841 cccttatctc ttccgggggg tatcccaggt tttcggggcc aaaggctaca tcctcttcca
    12901 caatggttgc aaccagttga ttgtcgggat tttgaaagac catacccgcc gtctggcgaa
    12961 tatcccagac catttcgtca tcgctggtat ccatgccctt gattaaaata attcctctat
    13021 ccggttttat cagcgcgtta atatgctttg ccagggtgga tttgcctgat ccattatgtc
    13081 cgatcacggc tatgaactcc ccctttttaa catccaggga gacatccttt aaggccgcga
    13141 tctcctcttc ttcgtagtta atatagctaa agcccacatt gcttgcttct attattgatt
    13201 ttatgatacc caccccctct ctgattcttg aatacaactg attccccgta ccatctattc
    13261 tacgggctta atgcacgttt tgcataaaaa ggctaacctt ggggattttt ttggcggcct
    13321 cggaagctat cgtacatcac aatcctacat caagctccag caatccaact tccaacttcc
    13381 aacctccaac ttctaacttc caacttccaa taggtaaaaa gatggattaa gttccccaac
    13441 ttaatcctct cccatcaaaa atatttatct taagttaaac aagctcgatg atgacttcct
    13501 cggcgccgtc cccccggcga ggtccaagct ttaaaattct tgtgtagccg ccgcctgaat
    13561 ttagttcctc gtttctcttt ctgtacttag ggccgtattc gctgaacatc ttctcgatga
    13621 ggggatgatt tacatccttt gttctctcac gataatcctc tttgttctcg tccttgcccc
    13681 ttatttcctg gtaatcgtaa agataggcca tcatctttct tctggcgttc agtctcgaag
    13741 gaaggtcatt tactacctcc tttgtaacgg tctgccctct ttcgttgttc atctctttag
    13801 ttaccttgat ggtcttatca tattccttta tggccaaggt aatcatcttt tcagcaatgc
    13861 ttcttacttc ctttgcacga gctccagtgg tctttatcct accgtgccac aaaagctcag
    13921 taacctggtt tctaagcagg gccttgcgct ggtcgctggg cctgctcagc tttctatgtc
    13981 ctggcatatg aacacccctc cttcacctaa ggtcgccccg caaaatgggt tttagcggag
    14041 cctgttgtac tattcatcac tttttttaag gctcaaatcc aaaaccgcca gcttttgttc
    14101 cacttcctcc agggattttt tccccagatt ccgtaccttc atcatatcct ctgccgtttt
    14161 ctgtatgagc tcgtctaccg tattgatacc cgccctcttc aggcagttat aggagcgaac
    14221 agaaagatcc aattcttcga tggtcatctc aaggaccttc tctttcttgt cctcttcctt
    14281 ttccaccata atttcgactt cattgacatg ctccgttaaa tctataaaga gcatcagatg
    14341 ttcgttcatg atcttagccg ctaggcttgc tgcctcatca gcctttaggc tcccattggt
    14401 ccaaacctcc agagtcaact tatcaaagtc cgtgacctgc cctacacgag tatcctccac
    14461 atgaaagttt acccttctta tgggggtaaa tatggaatcg atggggataa gtcctatggg
    14521 ttggcccgac tttttatttc tttcggcggg aacatatccc ctgcctcttt cgatggctat
    14581 ctccatatag agccttgcgt cctcgtttaa ggtggcaata tgcttgtcac cgtttaggat
    14641 ctccacgcct acatcggtta agatatccgc accggtcagc tcccccgggc cctggatgtt
    14701 aacggtaacc accttagcct cgtcctgttc catctttatg acaagttcct taaggtttag
    14761 tattatttca accaagtcct ctttaatgcc gggtatggta gaaaattcat ggagcacgcc
    14821 ttcaaccttt atcgaagtaa tagcggcacc cggcagcgat gacaatagaa ctcttctcag
    14881 ggaattaccc aaggtggttc caaatcctcg ctctaagggc tccaccacaa acttaccata
    14941 ggtgccgtcc tcttctaatt caattaattc aattttgggc ttttcgatct ctatcattaa
    15001 tacaaaccct cctcaaaaac ttttgatccg cttattccga gggcaaacaa tccaggagta
    15061 ttcatcctat ctggaataca actcaacaat taagtgctcc tgaattggga catcgatgtc
    15121 ttctctagcg ggcagggcag atactgttcc ttctaacttt tcataatcta cctgtaacca
    15181 gggcactacc gtcttgcctg cgccttctct taaggcttta aagtgctcta tttctgtggc
    15241 tgctttttgt ctcaccgcga ttatttcgcc aacgtcggtt aagtaggaag gaatatctac
    15301 tcgtttcccg tttacagaaa tatggccatg gagtacgaac tgtctggcct gggctctgga
    15361 ttctgcaaat cctaacctat acaccacatt gtccagtctt ttttccaaca gctgaagcag
    15421 gttctcaccc gtgattcctc tcatacgctc agcctgacca taatagcgtc taaattgttt
    15481 ttccaagacc ccgtatattc tcctggcctt ctgcttctct ctaagctgga tcccatattc
    15541 cgatgccttt ttccttcttt ggccatgctg tcccggcggt gtgggtctgc gggaaatggc
    15601 acatttatcg gaataacaac gatccccttt tagatatagt ttgattccct cccggcgaca
    15661 tagccgacag acggaacctg tatatttagc catctatcta cacctccatc aaactcttcg
    15721 tctcttcggc ggcctacacc cgttgtgggg aataggcgta acatccttta tcatgctgac
    15781 ttccaaccct gttgcctgca aagatcggat tgcggcttcc ctgccggcac ccggaccctt
    15841 tacaaagact tccacggttc tcagaccatg ctccattgcg gccttggcgg cggtttcagc
    15901 agccatctgt gcggcaaaag gggtgctctt tctggatccc ctaaagccca gcccgcctga
    15961 gctagcccat gaaagtgcat tgccggccgt atcggtaata gtaactatag tattattgaa
    16021 agttgagcga atatgtgcag cgccccgttc aatattcttt ctttcacggc gtcttctggt
    16081 tctacgcact tgctttctag ccatttctga ccctccttat ttatttctgc gaatgccaat
    16141 ggttctcttg gggcccttgc gggttcttgc attctgttta gtactctgtc cacggacagg
    16201 cagacccctg cgatgcctaa taccgcgata gcatccgatc tcgccaaggc gttttatgct
    16261 aagggaaacc tcacgtcttg cgtcaccttc taccgtgaca tttctgtcga tataatccct
    16321 gagtctatta acttccactt cagaaagatc cctaatcctt gtatcgggat ttatatccgt
    16381 tgcggccaat atttcattag atttagacct gcctattcca tagatatagg tcagcccgac
    16441 ttccacacgt tttcctctgg gcaggtcaat tccggcaata cgcgccatcg ttacacctcc
    16501 atattaatga ttattccata tttt
//
LOCUS       DASRNX010000107         4311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_21759,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000107 DASRNX010000000
VERSION     DASRNX010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21759"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..621
                     /locus_tag="VFD89_04245"
     CDS             145..621
                     /locus_tag="VFD89_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HZJ57432.1"
                     /translation="MARPRIRRKVCCIPESTLFGPLGNVDLNEEIIMALEEYETIRLI
                     DFEDLTQEECSERMKVARTTVQRIYSDARKKLAQVLVYGKSLRIEGGDYKLYDDEEKA
                     YGCGRCRRRRQGQGQGMGYGQGPGYGQGPGYGQGPGYGQGPGPGRGRGYGQGRGRG"
     gene            651..1019
                     /locus_tag="VFD89_04250"
     CDS             651..1019
                     /locus_tag="VFD89_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HZJ57433.1"
                     /translation="MKIAIPVNAKSMDKGVCPSFGRAPYFLIYDTETKQGEFIDNNAA
                     SSQGGAGIKAAQLIVDSEAHALITPRCGENAASVIKAGQVKMYRSIDGSIEENIDALQ
                     KDELSILDEIQAGFHNHGGR"
     gene            1021..1884
                     /locus_tag="VFD89_04255"
     CDS             1021..1884
                     /locus_tag="VFD89_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ57434.1"
                     /translation="MKIAVLSGKGGTGKTLLSVNMASAIGNSRYIDCDVEEPNGHLFF
                     KPQKVTSREVAVKIPVVDKELCDGCHRCVDFCRFNALALVGDNLMVFEEICHSCGGCL
                     LVCPKGALKEKEKAIGRIECGVSGGVQVATGILNTGEASGVPIIDALLDTKWDPAEFT
                     FIDCPPGSACIVMESIKDADYCILVAEPTLFGVHNLDMVYELVKLFNKPHGVVINKYF
                     DGENPAEKFCIEKGIDILGKIPFDKELGAMNSEGLIVARESEKYRQIFMDLLQKVIGG
                     VHHEAAVNPQR"
     gene            1856..2719
                     /locus_tag="VFD89_04260"
     CDS             1856..2719
                     /locus_tag="VFD89_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ57435.1"
                     /translation="MKQLLILSGKGGTGKTTIASAFISLGRLRAYADCDVDAPNLHLV
                     MKQDAEPARKDFFGLDKAVIDQSQCIKCDICRQNCRFNAIKADEGYRVDYYACEGCSV
                     CEFVCPQGAISMEPAVVGELMLYAGQDTIFSTAQLKMGSGNSGLLVTEVKKQIKDGDI
                     AADLAIIDGSPGIGCPVIASITGVDMVLIVAEPSISGISDMERIIKTGENFQVKMAVC
                     INKYDTNQNNTEKIETFCSEKGIPLLGKIPFDSQAIWATNSGRSITDIDCRAGHAAKD
                     IFERTMGILFD"
     gene            2761..3600
                     /locus_tag="VFD89_04265"
     CDS             2761..3600
                     /locus_tag="VFD89_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZJ57436.1"
                     /translation="MSENCNKDCESCGEECNERTAPKTDFREKPHELSNIKKVIGVVS
                     GKGGVGKSLVTSMLAVTMNRRGHSTAILDADVTGPSIAKTFGITGKATGNEMGMFPIR
                     STTGINIMSINMLLANETDPVIWRGPIIGGTVKQFWTDVIWGDVDYMFIDMPPGTGDV
                     PLTVFQSIAIDGVVVVASPQELVSMIVSKAVKMAEMMKIPIIGLVENMSYFECPDCHK
                     RHEIFGESHIDEIAHEHNIEVLAKLPMDTKIAKACDRGMIELYEGDWMDDIAKVLEEQ
                     IRK"
     gene            3621..3959
                     /locus_tag="VFD89_04270"
     CDS             3621..3959
                     /locus_tag="VFD89_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HZJ57437.1"
                     /translation="MKIAVASEGKNITQHFGHCENFNIYYIEDKKIVKSESIPNPGHR
                     PGFLPNFLNNLGVNVIISGGMGGGAAEIFRENGIEVVTGARGDAKEAAEQYSKGNLEF
                     TGQICDEHNR"
     gene            complement(3964..>4311)
                     /locus_tag="VFD89_04275"
     CDS             complement(3964..>4311)
                     /locus_tag="VFD89_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HZJ57438.1"
                     /translation="IFGLPGLGRRGIKLTYITDTRPIDTIPSFVNESDLLVCEGTYGD
                     DGNLQKAIENKHMTFREASELAVRGNVKEIWLTHFSPAMDEPELYFKNATDVFPNTVL
                     GYDGITMDLKFPL"
BASE COUNT         1274 a          819 c         1187 g         1031 t
ORIGIN      
        1 atggatgtct aggcaaatca ttttcgtata gaacatggtt cttcttcatt agggagcttc
       61 atgtagaaga ataatccata gatagggtgt aagacagcag ggggacgccc ctgttgtttt
      121 atggcagaat ggggtgaata agatatggca aggcctagaa tacgtagaaa ggtatgttgc
      181 ataccagaga gtacactttt tggaccctta gggaacgtag acttaaacga agaaataatt
      241 atggcacttg aggagtacga gaccattcgc ctcatcgatt ttgaagattt gacccaggag
      301 gaatgctccg agaggatgaa ggtggctagg accacagttc aaaggatata cagtgatgcc
      361 aggaagaaac tggcccaggt gctggtatac ggaaagtcat tgaggattga gggcggcgat
      421 tataagctct acgatgatga agaaaaggca tatggctgcg gcaggtgccg cagacgcagg
      481 caaggccaag gtcagggcat gggctatggc cagggtccgg gctatggcca gggtccgggc
      541 tatggacagg gtccgggcta tggacagggg ccgggaccgg gacggggtcg tggctatggg
      601 caagggagag gccgaggata aaaattatta ataaacgggg aggataaaaa atgaagatag
      661 cgataccggt aaacgcaaaa tccatggata aaggggtatg cccatccttt ggaagggcac
      721 cttattttct aatatatgac acggagacca aacagggcga gtttatcgat aataatgcgg
      781 catccagcca agggggtgca gggataaaag ctgcgcaact gattgtagac agcgaagccc
      841 atgcattaat taccccaagg tgtggcgaaa atgcagcttc tgttataaag gccgggcagg
      901 ttaagatgta caggagcata gacggttcca tagaggagaa tatagatgcc ctgcaaaagg
      961 atgaattatc aattctagat gaaattcagg caggttttca taatcacggg ggaagatagg
     1021 gtgaagatag ctgttttaag cggtaagggc ggtacgggaa aaacgcttct atccgttaat
     1081 atggcatcag caataggaaa ttcccgctat attgattgcg atgtagaaga acccaacggc
     1141 catctctttt ttaagcccca gaaggtcacc agccgggagg tcgcggtaaa gatacccgtc
     1201 gtagacaaag aattgtgtga tggatgccat agatgtgtag atttttgcag atttaacgcc
     1261 ttggccttag tgggagataa cttgatggta ttcgaagaga tatgccattc ctgcggggga
     1321 tgccttttgg tttgtcctaa aggggcattg aaggagaagg aaaaggctat tggtagaata
     1381 gaatgtggtg tgtctggggg ggttcaggtg gctaccggta tattaaacac cggggaggca
     1441 tcgggagtgc cgataatcga tgctctactg gacacgaaat gggatcccgc agagtttacc
     1501 tttatcgatt gtcccccggg cagcgcatgc atcgttatgg aaagcattaa agatgcggac
     1561 tattgtatat tggtagcaga gcctaccttg tttggggttc acaacctaga tatggtctat
     1621 gagctggtaa aattgtttaa taaaccccat ggggtagtaa tcaacaagta ttttgatggt
     1681 gagaatcccg cggagaaatt ttgtattgag aagggaatag atatcttggg caagatcccc
     1741 tttgataagg aattgggagc aatgaattct gagggattaa tcgtcgccag ggaaagcgaa
     1801 aagtacaggc agatatttat ggacttactt caaaaagtca ttgggggtgt acaccatgaa
     1861 gcagctgtta atcctcagcg gtaaaggggg tacgggcaag actaccatag ccagcgcctt
     1921 tattagcctt ggccgcctca gggcctatgc agactgcgat gtggacgccc ccaacctgca
     1981 tttggttatg aagcaggatg cagagcctgc tagaaaggac ttttttggtc ttgataaggc
     2041 tgttatagac cagagccaat gcataaaatg cgatatttgc aggcagaact gccgatttaa
     2101 tgcaataaag gcggatgagg ggtatagggt ggattattac gcctgtgaag gctgcagcgt
     2161 atgcgaattt gtttgtcccc agggggctat ctccatggaa cccgccgttg tcggggaatt
     2221 gatgctgtat gccgggcagg atacaatttt ttctaccgct cagctgaaga tgggcagcgg
     2281 taattcgggc cttttggtaa cagaggtaaa aaaacaaata aaggacgggg atatagccgc
     2341 ggatctggct atcatcgacg gttcccccgg tatcggatgc cctgttatag cctctataac
     2401 cggcgtggat atggttttaa tagttgccga gccctctatt tcagggatca gcgatatgga
     2461 gcgaatcatt aaaaccggcg agaactttca agtcaagatg gcggtatgta tcaataaata
     2521 cgatacaaac caaaataata cagaaaagat agagaccttt tgcagtgaaa aaggaatccc
     2581 cctattgggc aaaatcccct ttgattcaca ggctatatgg gctacaaata gcggccggag
     2641 catcaccgat atagactgta gagctggcca tgccgctaaa gatatctttg agagaaccat
     2701 ggggatattg tttgactaat cgtcatccga caacattaaa aagacaaagg agacaatctc
     2761 atgagcgaaa attgtaacaa ggactgcgag agctgcggcg aggagtgcaa cgagaggact
     2821 gcccccaaga ctgactttag ggaaaaaccc catgagctca gcaatataaa gaaggtaatc
     2881 ggggttgtaa gcggaaaagg cggggttgga aagtcattgg tgacatccat gcttgccgta
     2941 actatgaata ggagaggaca tagcaccgca attttggacg ctgatgtaac aggaccgtcc
     3001 atagctaaaa catttggaat aacggggaag gctacgggga atgagatggg gatgttcccc
     3061 attaggagca ctacggggat aaatataatg tcaataaaca tgctcctggc aaacgaaacc
     3121 gatcctgtca tatggagagg ccctattata ggcggcaccg ttaagcagtt ctggaccgat
     3181 gtcatctggg gagatgtgga ttatatgttt atagatatgc ccccgggaac cggggatgtt
     3241 cccctgactg tttttcaatc catagccata gacggggttg tagttgtagc ctcaccccaa
     3301 gaactagtat ccatgatagt gtccaaagcc gtaaagatgg cggaaatgat gaagataccc
     3361 ataataggct tagttgaaaa catgtcgtat tttgagtgtc ccgactgcca taaaagacat
     3421 gaaatattcg gtgaaagcca tatagatgag attgctcatg aacataatat agaggtctta
     3481 gccaagttac ctatggacac aaagattgcc aaggcctgtg ataggggtat gatcgaactg
     3541 tatgagggcg attggatgga tgatatcgcc aaggtacttg aagagcaaat tcgaaagtaa
     3601 tatataagga gggatttaca ttgaagatag ctgtagccag tgaaggcaaa aatattaccc
     3661 agcatttcgg gcattgcgag aactttaata tctattacat agaggataag aagatagtta
     3721 aaagtgaatc gattcccaat ccggggcaca gaccgggttt tttgcccaac tttctaaata
     3781 atctgggggt gaatgttatt atctcaggcg ggatgggcgg cggagccgca gagatttttc
     3841 gtgaaaacgg cattgaggta gtaacagggg ccagggggga cgcaaaagaa gccgctgaac
     3901 aatactctaa gggcaatttg gaatttaccg gtcaaatctg tgacgagcac aatagatgat
     3961 ccctcagagt gggaatttta ggtccatggt aatgccatca taccctaaga cggtattggg
     4021 gaaaacatct gttgcatttt taaaataaag ctccggctca tccattgcgg gactaaaatg
     4081 ggtgagccag atctctttta cattacccct aacggccaat tccgaggctt ctcgaaaagt
     4141 catatgctta ttctctatag ccttttgaag attcccgtca tctccgtagg tcccctcgca
     4201 tacgagcaaa tcgctttcat tgacaaagga cggtatggta tcaataggcc gggtatcggt
     4261 aatataggtt agctttatcc cccttcgccc tagccccggc agaccaaaga t
//
LOCUS       DASRNX010000108         3976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_393023,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000108 DASRNX010000000
VERSION     DASRNX010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393023"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..922
                     /locus_tag="VFD89_04280"
     CDS             143..922
                     /locus_tag="VFD89_04280"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HZJ57439.1"
                     /translation="MKIKKDIWILTFVGILAGFFVIRAFDAQHPQLGFSNMLSPHTLD
                     RYFQDRSIARIEQYVKHTKALEESRDLLLEEIRELDKIKSEHEEIAAARLDKGEQIRR
                     ELQDSRMVAGMLDIEGPGVEIIVDDRKRDTILDSDSDMIGYFIVHDSDLLEVVNELRA
                     AGAEAVAINGVRITGSSRISCGGPTINVGKEQRFTPPFVIHAIGDPEALTSYFQREDS
                     IRQTLLFWGLRFEMKQKDPVLIPRLIGDTDFRYAKPIREDE"
     gene            924..1286
                     /locus_tag="VFD89_04285"
     CDS             924..1286
                     /locus_tag="VFD89_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HZJ57440.1"
                     /translation="MLLPILGIIVGIVLGLLLPLHIPISYASYMSIAVLAALDSVFGG
                     IRAKFEDSFESDIFISGFFGNAFLAAGLAYIGDRLDVPIYLAAIFAFGIRLFQNFAAL
                     RRHFLAYLRRRSGMGGEK"
     gene            1607..2686
                     /gene="ftsZ"
                     /locus_tag="VFD89_04290"
     CDS             1607..2686
                     /gene="ftsZ"
                     /locus_tag="VFD89_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZJ57441.1"
                     /translation="MLEFDIDMDQFAHIKVVGVGGGGNNAINRMVQYGLKGVEFISIN
                     TDKQALHISQSNLKIQIGEKLTKGLGAGANPEIGQKAAEESKEDIQLALEGADMVFIT
                     AGMGGGTGTGAAPVVAETAKEMGILTVGVVTKPFLFEGRQRMTNAERGIVELKERVDT
                     LVTIPNDRLLQVIEKRTSMLEAFRIADDILRQGVQGISDLIAVPGLVNLDFADVRTIM
                     REKGLAHMGIGKSSGDSRAMDAAKQAIQSPLLETTIDGAKGVLLNITGGDNLGLFEVN
                     EAAELVAQAAHPDANIIFGAVIDDSLEDELRITVIATGFEQQKTQTSGELKRSKGRVV
                     EPQFQQQEHQEDDLVIPTFLRRGRS"
     gene            2870..3787
                     /gene="spoIIGA"
                     /locus_tag="VFD89_04295"
     CDS             2870..3787
                     /gene="spoIIGA"
                     /locus_tag="VFD89_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HZJ57442.1"
                     /translation="MVSVEYRYLDVIWLDNFIMNYILLWTVSKVSKDKTPQWRLCIAS
                     FIGAVYVVLLIISTNNILDFPITKMILSVCMVLIGYRYTSWPRLFRLIGLFYGITFAF
                     GGAAFGVYFFTKDILTIKKGVFYLKNFPAKIILTSSVAVAILIRLLWPKIRKRWLSSG
                     LIYSINIRCGETDFIIKGLLDTGNSLYDPVTQSPVIIVEYSKIQKALPTGIRDIFMQS
                     REHDLDYVAKVISGAQFNPDFRLIPYYAVGKPGGFLIGLRPNRVLISLGGDWHMGGDI
                     IVALYAGGLSRDGEYHALIHPEMVCTDKQ"
BASE COUNT         1129 a          781 c         1054 g         1012 t
ORIGIN      
        1 gggcggtgta gtggacaatc tgagccaaat tttggacatc tccgtcaaat cagaacagag
       61 gattgctgtt cccagctatg agggggccct aaactatagg tttgccgagc ctctccagga
      121 aaagaagtag tgggggatgc agatgaaaat aaaaaaggat atttggatac tgacctttgt
      181 tgggatactg gcaggcttct ttgtaataag agccttcgat gcccagcatc cacagctggg
      241 atttagcaat atgctctctc cccataccct ggaccgatat ttccaagaca gatccatagc
      301 caggatagag cagtatgtca agcatacaaa ggcccttgaa gagagtaggg acctcttgtt
      361 ggaggagatc agggagctgg acaagatcaa atcggaacat gaggaaatag ccgccgcaag
      421 gctggacaag ggggaacaga tccgccggga gttacaagat agcaggatgg tggcggggat
      481 gttggacatc gaagggccgg gtgtggagat tatcgtggat gacaggaaaa gggacaccat
      541 tttagacagc gattcggata tgatcgggta ctttatagtg cacgacagcg accttctcga
      601 ggtggtaaat gagcttaggg cagcgggggc ggaggcggtg gccataaatg gcgtaagaat
      661 taccggatcg tccaggatca gctgcggcgg gcctaccatt aacgtgggaa aggaacagag
      721 gtttaccccg ccttttgtga tccatgccat cggcgatcca gaggccttga cctcctattt
      781 tcagcgggaa gacagtatcc gccaaaccct attgttttgg ggattgaggt ttgaaatgaa
      841 gcagaaggat ccggttctga taccgcgctt aattggagat acagatttta gatatgcgaa
      901 acctataagg gaggatgaat aggatgttac ttccaatatt ggggattatc gttggaattg
      961 tactggggct cttattgccg ttacatatcc caataagcta cgcgtcatac atgtctattg
     1021 ctgtcctggc tgctctggac tcggtgttcg gcggaatacg ggccaagttc gaggacagct
     1081 ttgaatccga tatcttcatc tcgggatttt tcggaaacgc ttttttagcg gcagggctgg
     1141 catacatagg ggacaggttg gatgtaccca tatacttggc cgctatcttt gcctttggca
     1201 tcaggctctt tcaaaatttt gccgccctgc gccgacattt tttagcatac ctgcgaagaa
     1261 ggtccggaat gggcggggaa aaataattta taatgttatt ccgttttatg gggatttatg
     1321 ataacataat aaatatatta aatatttcga tcttaacgca gttgcactga atgatcttta
     1381 ggattcttcg gcaataataa cgagacggaa agatgtcccc catttctata agtggcggat
     1441 tcctattagt cgaaacagac gacgggtaaa aatcccccgt ctcgttgaaa ccagtgtgtt
     1501 gcaattgcag gcaattgctt ccgatgtttt aagggacatt tttctgttgt acaagccgat
     1561 atatagccga ttgtttgatg aaaaaggaat taggggagga agggaaatgc tggaattcga
     1621 tattgatatg gatcaatttg ctcatataaa agtggttggc gttgggggtg gcggaaataa
     1681 tgccataaat agaatggttc agtacggatt aaagggtgta gaatttattt ccattaatac
     1741 ggacaagcag gcattacata tatcccaatc aaacctgaag atacagatag gggagaagct
     1801 gaccaagggg ctgggcgccg gagccaaccc ggaaattggg caaaaggccg cagaggaaag
     1861 caaggaggat atccaactgg cgctggaagg cgcggatatg gtgtttatta ccgccggtat
     1921 ggggggcggc accggtacgg gggctgcgcc tgtagtagcc gagacagcaa aggagatggg
     1981 aattctcacc gtcggggtgg tcaccaagcc cttcctcttc gagggacgac agaggatgac
     2041 caacgctgag aggggtatag tagagctgaa ggaaagggtg gatacccttg tgactatacc
     2101 caacgacagg ctgttacagg ttatagaaaa acgcacctct atgcttgagg cctttaggat
     2161 tgccgacgat attttaaggc agggcgtgca gggaatttca gacctaatag ccgtcccggg
     2221 tttggtgaac ttagactttg ccgatgtgcg taccataatg agagaaaaag gccttgccca
     2281 tatgggcatc ggaaagagca gcggtgacag cagagccatg gatgccgcca agcaggccat
     2341 ccagagcccc ttacttgaaa ctaccataga cggggccaag ggtgttcttc tcaatataac
     2401 cggcggggac aacttaggcc tctttgaagt caatgaagcg gcggaactgg tggcccaggc
     2461 ggcccacccc gatgccaata taatcttcgg cgctgtaatc gacgattcat tggaggacga
     2521 gctcaggatt acggttatag ccacaggttt tgaacaacaa aaaacccaaa cttccgggga
     2581 gcttaagagg agtaagggca gggtagtaga gccccagttt cagcaacagg agcatcaaga
     2641 ggacgatctg gtaatcccta cctttttaag gcggggaaga agttaggcaa ggcccaataa
     2701 aaaaccaata aatatccggc acaaaactta tggataacaa ccatgtgtag aaacaagtga
     2761 caataaaaga gatttttgtc gcttgttttt tttatacgag cacacagttc tacaatattt
     2821 tgatacaggg tgttatataa taaggacaaa acgtggagaa agtgtggtcg tggtgtccgt
     2881 tgagtatagg tacttagatg taatatggct ggataatttc atcatgaact atatactctt
     2941 atggactgta tctaaggtat ctaaggataa aacaccccaa tggagattat gtatagcatc
     3001 gtttataggt gctgtatacg ttgtattatt gattatatca acgaacaaca tactggattt
     3061 tcccatcaca aagatgatcc tatccgtctg tatggtattg ataggatata ggtatacgtc
     3121 ctggcccagg ctctttagac ttatcgggct attctacggt ataacctttg cttttggagg
     3181 agccgccttc ggggtatact tctttaccaa ggatatactc accattaaaa agggggtatt
     3241 ttatcttaag aatttccccg ccaagattat actcacgtcg tctgtggcag tagccatttt
     3301 aatccgctta ttatggccta aaatacggaa aagatggctg tcctcgggtc taatctacag
     3361 catcaacata aggtgcgggg aaacggattt cataataaag gggctcttag ataccgggaa
     3421 ttccctgtat gaccctgtca cccaaagtcc ggtgattatt gtagaatatt ctaaaataca
     3481 aaaggctctg ccgacgggga taagggatat ctttatgcag agcagggagc atgatttgga
     3541 ttatgtagcg aaagttattt cgggagccca atttaatccc gattttcgct taatccccta
     3601 ttatgccgtg ggcaaaccgg gcggattttt aataggtcta aggcccaata gggttctaat
     3661 ttcccttggc ggggattggc atatgggcgg tgacataatt gttgccctat atgctggggg
     3721 gctgtcaagg gacggagaat accacgcact tattcatccg gagatggtct gtacagataa
     3781 gcaataggtc aactaaattt atcgacatga taagcacaac gggtttatgc actttcatgt
     3841 cgcagcatat ataaatcagg gaaaatgctt tacgacaccc aaggcgtatg aaaaaccttt
     3901 gtcgtaaagc acatatctac ttgattgtta aaaggaggac aacgatattg agtagaattt
     3961 ttattgataa gatcag
//
LOCUS       DASRNX010000109         4525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_73334,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000109 DASRNX010000000
VERSION     DASRNX010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73334"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="VFD89_04300"
     CDS             <1..1387
                     /locus_tag="VFD89_04300"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HZJ57443.1"
                     /translation="EDFVYFEGGEILKHLYVTEVFKEAQIIPLIYATALPNGVASRDT
                     YDYYADRILKILSENKDVDGVFLHLHGSLAVQGLGPDGDLGSGEYDLIKRIRKLIGSD
                     PIIGLALDTHANTDPRLPKLVNVMRNYRTVPHQDQDVTEQVVARHMIDCIKNKKKTVP
                     QFVRLPYAIHPEKALGREWPLSEIFARLDEMEKMEGVSVATLGIGMIWCDCETLASNV
                     AVTPSEDRYTRKAAELARELADYVYSFRDSFEFSQLPLSPYEAVKQSIDYKGSPVFVS
                     DSGDNTTGGAVGDHTILLREFLNSPDYKGKRIAVTGIWDEKAVARCMKHNEGDVISIK
                     IGRNYDDNTKAVAVTGTLKKKGHLYGFIGHEKDAVGAAVTISIGYLDIVLIDRPGSFI
                     TIGHFKGSGLDIEDYDVIVVKQGYLFQELRELAKLAILALTPGATNQIIEDLEYKRIV
                     PPVYPLKKLGI"
     gene            1428..2741
                     /locus_tag="VFD89_04305"
     CDS             1428..2741
                     /locus_tag="VFD89_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HZJ57444.1"
                     /translation="MTETRVSTKNPDSQIAPAKDYSLTVNGEEVFVYSCDVANYAVLS
                     GQGSMEVEITSVNPFKTVKVRPLISKIAPMVKGNKVSFTIGGPQKLSIEFDGDILNPL
                     FILINPVEKTKPNKGDDKVRYFESGKVYDIGQMELHEGDTVYIAEGAVVYGSFCAYRA
                     NNLTIKGRGILNGSKWHRQGSAQRQQMIKLVECKNVTIEGITIVDGPNWHVVPIACGD
                     VAIRDLNIITFVMTGDGIDVVGSENVSINNCFVRSNDDCVAIKAVDYQHESGHKNVKD
                     VRISDSVFWNAEFGNALEIGYETRCDEISNIVFENCDVIRSEFEGYESGGTFTIHNGD
                     RAHISNVRYDNIRVEDSREKLIDIKILFSQYSRDEERGQVSNITFNNIQVVDGPFPVS
                     IVRGYDADHMIRDITIKDMTVHGERIRSANQCRMVVELSKNVDFE"
     gene            2816..4273
                     /locus_tag="VFD89_04310"
                     /pseudo
     CDS             2816..4273
                     /locus_tag="VFD89_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
BASE COUNT         1362 a          912 c         1141 g         1110 t
ORIGIN      
        1 ggaggatttt gtatatttcg aaggtgggga aattctaaaa cacctttatg taacagaagt
       61 tttcaaagag gcccaaatca tccccttaat atatgcaaca gctctgccca atggagtcgc
      121 ctctagagac acctatgatt attatgccga ccgtatttta aagatactga gtgagaacaa
      181 ggatgtagat ggtgtgtttt tgcatcttca tgggagtttg gcggttcaag gcttagggcc
      241 cgacggggat ttgggttccg gtgaatatga tcttataaaa aggataagaa aactcatagg
      301 cagcgatcct atcatcggct tggcacttga tacccatgcc aatacagatc ctaggctgcc
      361 taaactagtt aatgtcatga gaaattatcg caccgtccct caccaggatc aggatgttac
      421 agagcaagta gtggcaaggc atatgataga ttgtatcaaa aacaaaaaga agacggtccc
      481 ccaattcgtg cgccttccct atgcaattca tccggaaaaa gccctcggtc gggagtggcc
      541 cttatccgag atatttgcac gactggatga aatggagaag atggagggag tatccgttgc
      601 aacattgggc atcggtatga tctggtgtga ttgcgagacc ttggcaagca atgtagcggt
      661 gaccccatcc gaggaccgtt atacacgaaa agcggctgag ctagctaggg aactagcaga
      721 ctatgtatat tcatttcgag atagctttga atttagtcag cttccgttat ccccctacga
      781 agcggtgaaa caatccatag attataaggg ttccccagtc tttgtttcag attccggtga
      841 taacactacc ggcggggcag taggggatca taccattttg ctgcgagaat ttctaaactc
      901 ccctgactat aagggtaaga ggatagcagt tacaggcatt tgggatgaaa aggccgtcgc
      961 ccggtgcatg aaacataacg aaggcgatgt aatttcgata aagataggca ggaactatga
     1021 tgacaacacc aaagcggtag cggtgaccgg gacattaaag aaaaagggcc acctgtatgg
     1081 atttataggt catgaaaagg atgcggtagg tgcagccgtt acaatctcta taggttactt
     1141 agacattgtt ttaatcgata ggcctggctc ctttataacg attggacatt ttaagggatc
     1201 tggtttagat atagaagatt atgatgttat tgtcgtcaaa cagggatatt tgttccaaga
     1261 attaagggaa ctggcaaaac tggctatctt ggctttgacc ccaggggcaa ccaatcaaat
     1321 aatagaagat ctagagtata aacgcatagt accgccggtc tatccgctta agaaattagg
     1381 gatataaggt agcatttaac caatacagag aagagaaggt gtatagaatg acagaaaccc
     1441 gggtatccac gaaaaatcct gattcacaaa tcgctccggc gaaggattat agtttaaccg
     1501 tcaacgggga ggaggtcttt gtttattcct gcgatgtggc caattatgct gtcctttctg
     1561 gccagggaag tatggaagtg gaaatcacat ccgtcaaccc ctttaagacc gtaaaggtaa
     1621 ggccattaat ttctaaaata gcgcctatgg taaagggcaa taaggtatcc tttaccatag
     1681 ggggtcccca aaaattgagc atagaatttg atggcgacat attgaacccc ctattcatac
     1741 taatcaatcc agtggaaaag acaaaaccca ataagggcga tgacaaggtc cgttattttg
     1801 aatccggaaa ggtgtacgat atcggtcaaa tggaactgca tgaaggggat accgtttaca
     1861 tagccgaggg agcggtagta tacggctcat tctgcgccta tagggccaac aacttgacca
     1921 taaagggccg tggaatacta aacggcagta aatggcatag gcagggcagt gcacaacggc
     1981 agcagatgat aaagctggtg gaatgcaaaa acgtaaccat cgaaggcata acaatagtgg
     2041 acggacccaa ctggcatgtc gtgcccatcg cctgtgggga tgttgccatt agggatttga
     2101 atataataac cttcgttatg accggcgacg gcattgatgt ggtgggcagc gagaatgtca
     2161 gcatcaataa ttgctttgta aggtccaacg atgattgcgt ggctataaag gcggttgact
     2221 atcaacacga atccggacac aaaaatgtaa aggacgtgag gatatcggac agcgtatttt
     2281 ggaatgcgga gtttgggaat gccctggaaa taggctacga aacccggtgc gacgagatta
     2341 gcaatatagt ttttgaaaac tgtgatgtca tacgcagtga attcgagggc tatgagtccg
     2401 gcgggacctt taccatccat aacggcgaca gggcccatat aagcaatgtc aggtatgaca
     2461 atatcagggt ggaggattcc agggaaaaac tcattgatat caaaatactt ttctcccagt
     2521 acagtcgtga tgaggaaagg ggccaggtca gtaatattac ttttaacaat atacaagtcg
     2581 ttgacgggcc ttttccggtt tcaattgttc gaggatacga tgcagatcac atgattaggg
     2641 atataactat taaggatatg acagtacatg gggaaaggat taggagcgca aaccagtgca
     2701 gaatggtggt agaattgtct aagaatgtag acttcgaata acagtttacg tttctcacat
     2761 tgaggttcta agcctactcg catcccatat taagacgaaa ggggaagaaa caatcatgga
     2821 cagaccaaac gtattgctca ttaccgtgga ccagatgagg ggtgattgtc tggggatatt
     2881 gggacatccc ctagtggaaa caccccattt agataccatg gcccatagag gggtcatgtt
     2941 taccaacgct tatacggcgg cacccagctg tatcgcctcc agagcagcta ttatgacagg
     3001 gctcgcacaa ggaaatcacg gaagggtcgg ttatgaagat aaggtcaggt gaaattatga
     3061 ccgttatatg gccggagaat ttgcggatgc aggctatcat acccaggctg ttgggaagat
     3121 gcatgtccat ccccaaagat tcctatgtgg atttcacaac gtattgcttc atgacggata
     3181 tcttcatgcc tacagaaaca aggatgttaa ggcttcggaa aattggttta acacggacga
     3241 ctatctgccc tggttgaggg agaagttggg ctatgatgcc gatatcatcg ataccgggct
     3301 tgactgtaac tcatgggtcg caaggccatg gatgtacccc gaacaccttc accctaccaa
     3361 ttgggttgta agcgaatcca tagacttttt aaggaggcgg gatcccagta aacccttttt
     3421 ccacatgatg tcctttgtca ggccccactc acccttggat ccgccaaaat actactatga
     3481 tatgtatata gacgaagata tgcccgaccc tccaacaggg gattgggaag tccgggacaa
     3541 tgacatgcag gacccgctgg tctataacgg atttggcggc aggttaagta aaaaggctct
     3601 aaagagggca agggcaggct attatgggag tatcacccat atagatcatc agataggccg
     3661 attcttgcaa gccctgcacg atgcgaatgt cctaaacgat accattatcc tgtttacttc
     3721 cgatcatggg gatatgatgg gggatcacga ctggtttaga aaatccctgc cctacgaggg
     3781 gagtacttca atccccttta tcatatatga tccgggcaac aatctggcgg ggttaaagac
     3841 cggcacatcc gtaaataacg tagttgaact aagggatatt atgcctacct tactcgatgc
     3901 cgccggcata actatacccg atggcattga tgggaaaagt gtattagccc tagcgagggg
     3961 ccgtgaatct gaatggaggg agtatctcca tggcgagcat tccttcgggt ccgcctcaaa
     4021 ccattatata gtcaccgaaa gggataagta tgtatggtat tcccagacag ggcaggaaca
     4081 gtatttcaat cttcacgatg acccccaaga attgcataat gcaatagatg atgtaaagca
     4141 caaggacagg atagaacttt taagggggca ccttattatg gaactgaggg acagggagga
     4201 aggctatgta gccggtacac aattaatagc gggcagaccc ataagaagca gcctttccca
     4261 tattaagaac tgatgggtac ccataagaag caacctttcc catattaagg attgatgagg
     4321 gttttttaaa actgggaccg cccatggagc caattaggaa aacggttgtc aaagagtaca
     4381 ggctggggtt acaggagata tgatgattac tatacaccgc ctgttatttg acccgaataa
     4441 ataatgtaaa atactattat gttctgaata agtcagacat acccatgggc tgggtctgtc
     4501 ttatccgtta agaataatta aaaaa
//
LOCUS       DASRNX010000110         8995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_552249,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000110 DASRNX010000000
VERSION     DASRNX010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8995
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552249"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFD89_04315"
     CDS             <1..233
                     /locus_tag="VFD89_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57445.1"
                     /translation="YRELDPCINGFKIRLMLKVFEELQFIRVDSDNKYVKILYHKNPS
                     HQKLCESDLFAYYNRWISGVGLFPTGDKHNIK"
     gene            259..780
                     /locus_tag="VFD89_04320"
     CDS             259..780
                     /locus_tag="VFD89_04320"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010076966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HZJ57446.1"
                     /translation="MNLMDKIRIIPDFPKTGISFKDITTLLHDREAYKYTVESLVGVC
                     KEWNPDLVVGPEARGFVIGAPLAYALGVGFVPVRKPGKLPGKTELFEYDLEYGKDALE
                     IHSDAITKGQRIVIVDDLLATGGTSLATARLVERLGGIVVGMTFVIELSYLGGRERLG
                     DYNIHTLVQYEED"
     gene            1001..3109
                     /locus_tag="VFD89_04325"
     CDS             1001..3109
                     /locus_tag="VFD89_04325"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZJ57447.1"
                     /translation="MRKVMDAYTFAFDAHKSQLRSSGEPYIIHPLEVAQILMEMGLDI
                     DAVAAGLLHDVVEDTGVSVEELTILFGAEVAGLVDGVTKLGKIAYKTKVEQQAENLRK
                     MFLAMAKDIRVIIIKLADRLHNLRTLEYVDEEKQREKAYETLEIYAPLAHRLGIFKIK
                     WELEDTSLRYIDPNGYYDLVEKIAVKRKERENYIQEIIETLEVKLGEISIEAEIEGRP
                     KHFYSIYKKMYMQHKDFDQIYDLLAIRIIVDTVKDCYGVLGVTHTLWKPIPGRFKDYI
                     AVPKPNMYQSLHTTVIGPRGELFEIQIRTWEMHRTAEYGIAAHWKYKEGKKTSTELDQ
                     KLAWLRHLLEWQGDLKDPREFMETLKIDLFTDEVFVFTPKGDVIDLRKGATPLDFAYA
                     IHSAVGNKCTGAKVNGRIVPLDYNLQTGDIVEILTSSSSGGPSRDWLNIVKTSQAKSK
                     IRQFFKREGREENIAKGKEMLEREARRQGYSLSQLLHKDWSESVWKKYGFHSAEDMYS
                     ALGYGGITTNQILFRLIDEYKKANKPDVEDLIEKEKTKPQKEHAGNDKGVKVKGIDNI
                     MVRFSKCCNPVPGDDIIGYVTRGRGVSIHRRDCINLQDPSVENHRLIETSWIDEYSAS
                     FSSEIQILARDRQMLLADITKAISDMKITVTAISARTTKNKQAIINIMMEINDVEQLK
                     RIIKQFKRVDEVMDVFRVKA"
     gene            3278..3727
                     /gene="dtd"
                     /locus_tag="VFD89_04330"
     CDS             3278..3727
                     /gene="dtd"
                     /locus_tag="VFD89_04330"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZJ57448.1"
                     /translation="MRAVVQRVSTARVRVGQEITGQIDRGLLVYLGVGIQDEARDLDY
                     ILDKVTKLRVFEDQDGKMNLSVEEVGGAILVISQFTLYGDCRRGRRPSFTDAAGPDKA
                     EDFYNQFIHRIREMGISVETGIFKAHMHVESLNDGPVTILLDSRRTF"
     gene            3747..4376
                     /locus_tag="VFD89_04335"
     CDS             3747..4376
                     /locus_tag="VFD89_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZJ57449.1"
                     /translation="MKIIAMPVGILGANSYIIYHEITGDAVVIDPGGDGETILGEISS
                     RELEVKYILLTHGHFDHIHGVGSLKERTGAKVAIHKEDAGSLGDIHKNLSSFMGVESV
                     QPEADIMLEHGQRLEFGDIILKIIHTPGHSPGSICLRFDNIVFTGDTLFKGSIGRTDL
                     VGGELGQILGSIKDRLLTLDDGTIVYPGHGPMTTIGDEKLTNQFLRGLI"
     gene            4378..5871
                     /gene="hemZ"
                     /locus_tag="VFD89_04340"
     CDS             4378..5871
                     /gene="hemZ"
                     /locus_tag="VFD89_04340"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HZJ57450.1"
                     /translation="MTIYILTSIQEYSNELAELARAFYPGRVVKTDTGNPEDLLPGDL
                     LFRCEFQTVGDTTKIAVKLFKGMETSSVFSKTHRLRPDTTPMTKRRDIKNCMKQMLYD
                     ILMDLTDRSLDWGALTGVRPTKIAHGLIDEGLDRNKVLKLLYDDYRVSQKKAELLHGI
                     AKIQRPFFCGNHPNRISVYINIPICPTKCLYCSFPSATMDQCAHLVDDYITALEYEME
                     TMSILAREKGIEVETVYIGGGTPTSLNTVQLERVLRAVREFWGWSPWKEYTVEGGRPD
                     TMSGDKLRVLKDHSVTRISINPQSMNDKTLGAIGRNHKAHEIVECFYRARDVGFDCIN
                     MDVIVGLPGEGIEEGRRTMGSIKKLAPDNLTVHTLAVKRASRLKDNLERFALTDERLA
                     AGMMDIFYEGAGSMGMKPYYLYRQKYMLGNMENVGFATPGKESIYNMQIMEDRQTIWA
                     FGAAAITKVIYPTMGRIERAANVKNIGDYIDRTEEMVQRKLKICTYS"
     gene            5886..7151
                     /gene="hisS"
                     /locus_tag="VFD89_04345"
     CDS             5886..7151
                     /gene="hisS"
                     /locus_tag="VFD89_04345"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZJ57451.1"
                     /translation="MNIKAPRGTKDVLPDESYRWHYLEGIIREITQLFGYKEIRTPVF
                     EHTELFVRGVGDTTDIVQKEMYTFLDKGDRSITLKPEGTAGAVRAYIENSLYGMAQPV
                     KMYYLTPVFRYENPQAGRLREHHQFGVEVFGAKEPTIDAEIIMLATKLFERLGLSGLE
                     LNINSIGCPQCRPGYHDALREYLKGHLGELCHTCNSRYERNPLRILDCKTEKCSAIVK
                     DAPIILDYLCKECSAHFNGLQDYLKAASVEYNINPMIVRGLDYYSKTVFEIISTEIGA
                     QGTVCGGGRYDPLVKECGGPDTPGAGFGLGLERLLLALDGQGLEIPKAPGRDVFFVTI
                     GEAARDKAFELLDTMRNEGILADMDHVGRGVKAQFKYADKADVTWVCILGDDELSRGE
                     VKVRAMADGREETVSQANLAEYFKSKKER"
     gene            7158..8960
                     /gene="aspS"
                     /locus_tag="VFD89_04350"
     CDS             7158..8960
                     /gene="aspS"
                     /locus_tag="VFD89_04350"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZJ57452.1"
                     /translation="MGESISGLKRTHMCGELTSNDLGQNVVLMGWVQRRRDLGGLIFV
                     TLRDKTGIIQLVFDQDESKALFHKAARLRSEYVIASAGEVIARAPEAINPDMPTGSIE
                     IKVKELRILSGAETPPFYIEDNINVGEALRYRYRYLDLRRPEMQNNLLVRHRIANSVR
                     DFLNGKGFMEIETPMLTKSTPEGARDYLVPSRVHLGKFYALPQSPQLFKQLLMLAGYD
                     RYYQLARCFRDEDLRADRQPEFTQIDIEMSFVEIEDILAINEEMIADVFRRVLGIEIS
                     LPLKRITYKEALERYGLDKPDTRFGLELMDVSALVADSDFKVFSNAVGSGGSVRAINV
                     KGCGNKFSRREIDSLGEYVKDYRAKGLAWIHYSEDGIRSPIAKFLAREEMDAIIKYMD
                     AEVGDLLLFVADRDEIVFQALGNLRQKLGRQLDLIPTDRYDLLWITEFPLFEYDEGEN
                     RFVAKHHPFTSPMDEDIDLLDTRPGETRAKAYDMVLNGNEIGGGSIRIHDIEVQERMF
                     KALGFTLEEAWEQFGFLMEAFKYGTPPHGGIAYGLDRIAALLAGRPTIRDVIAFPKVQ
                     NASDPMTNAPTAVDSRQLEDLHIAIAKNGEDKDV"
BASE COUNT         2694 a         1636 c         2423 g         2242 t
ORIGIN      
        1 gctatagaga attggatcct tgtataaatg gctttaaaat tagacttatg ctgaaggtct
       61 ttgaagaact acaatttata agggttgatt ctgataataa gtatgttaag atcctatatc
      121 ataagaaccc aagccatcag aagctatgtg agagcgatct atttgcttac tataaccggt
      181 ggattagcgg tgtgggctta tttcctacgg gagataagca taacataaaa tgaatcgcaa
      241 ttttaaggag gaaatttcat gaatctcatg gataaaatca ggattatccc ggattttcct
      301 aaaacgggta tcagttttaa ggatataact accctactac atgataggga ggcctataag
      361 tacaccgttg aaagcctggt gggggtttgt aaagaatgga atccggatct ggtagttggc
      421 cctgaggcca ggggctttgt aattggtgct cctctggcct atgcattagg ggttggattc
      481 gtgccggtcc gaaagcccgg aaagctgccg gggaaaacgg aattatttga atatgatctg
      541 gaatacggga aggatgctct ggaaattcac agtgacgcga taacgaaagg tcaaagaata
      601 gtcatagtcg atgatctgct ggccaccggg ggtacgtcct tggcaacagc gagacttgta
      661 gaaagactgg gtggcatagt ggtagggatg acctttgtaa ttgagctttc ctatcttggg
      721 ggaagggaaa gactggggga ttataatata cacacattgg tccaatatga ggaggattga
      781 cccgccgaga agctatggta tttcgctatt atcgatagac ctaagaatct tttgaagttt
      841 cagaaggctt gaattcccca catagctatc taagacattg aataatgtgt tttcgaaact
      901 tccctaatat aattaaaagg acgtatggga gatggtggaa tttaatgaaa aattggataa
      961 attagaggcc agggcggaac agctatttgg acatgagggt atgaggaagg ttatggatgc
     1021 ctataccttt gcttttgatg cccataaatc ccaactgaga tcttctggag aaccctatat
     1081 aatccacccc ttggaggtgg cgcagatact tatggaaatg ggtttggata tagacgccgt
     1141 tgcggcgggt ctgctccatg atgttgtaga ggataccggg gtctcagtag aggagctaac
     1201 gatcttgttt ggggctgaag tggcagggct tgtggatggg gttaccaagc tgggtaagat
     1261 agcttacaaa accaaggtgg aacaacaggc tgagaatctt cgaaagatgt ttttggctat
     1321 ggctaaagac ataagggtta ttatcattaa gctggctgac cgtctccata atttgaggac
     1381 cctcgaatac gtcgatgaag aaaagcaaag ggagaaggcc tatgagaccc tggagatcta
     1441 cgctccccta gctcacaggt tgggtatttt caagatcaag tgggaactcg aagatacttc
     1501 ccttaggtat atagatccca acgggtatta tgacttagtt gagaaaatag cagtgaagag
     1561 aaaggaaaga gagaattata tacaagagat tatagaaacc ttggaagtaa agctggggga
     1621 aattagtatc gaggcggaaa tagaggggcg gcccaagcat ttttatagca tatataagaa
     1681 gatgtatatg cagcataagg actttgacca aatttacgac ttattagcta taagaattat
     1741 tgtcgacacg gttaaggatt gttatggtgt cctgggagta acgcataccc tatggaaacc
     1801 tatcccggga agattcaagg actatatcgc tgtccctaaa cccaatatgt atcaatccct
     1861 tcatactacg gttattgggc caaggggcga actcttcgag atacagataa gaacgtggga
     1921 gatgcatcgt actgctgaat acggaattgc cgcccattgg aaatacaaag agggcaaaaa
     1981 aacctccacg gaattggatc aaaagttggc atggcttagg cacctattgg aatggcaagg
     2041 tgatctaaag gatcctaggg aatttatgga gaccttgaaa atcgacctat tcaccgacga
     2101 agtatttgta tttacaccaa aaggtgatgt aattgatctg cgtaagggtg ccactccatt
     2161 ggattttgcc tatgctattc atagtgctgt cggaaataaa tgcaccggtg ccaaggtaaa
     2221 cggtagaatt gtgcccttgg actataattt gcagaccggg gatatcgtgg aaatcctcac
     2281 gtcatcatcc agcgggggac ccagtcggga ctggctcaat atagtcaaga ccagccaggc
     2341 gaagagcaag ataagacagt tcttcaagag agagggcagg gaagagaaca ttgccaaggg
     2401 taaggaaatg cttgaaaggg aagccagaag acaggggtac tcgctctctc agctactgca
     2461 taaggattgg tcggaatctg tatggaaaaa atatggcttt cattctgccg aggatatgta
     2521 ttcggcccta ggttatgggg gtatcaccac taatcaaatt ttatttcgat tgatagatga
     2581 gtacaagaag gccaacaaac cagatgtcga ggatctaatt gaaaaggaaa aaaccaaacc
     2641 ccaaaaagaa catgccggta atgacaaagg tgttaaggtg aagggcattg acaatataat
     2701 ggtccgtttt tcaaagtgtt gtaacccggt ccccggggac gatattatag gctatgttac
     2761 taggggcagg ggagtatcaa tccataggag ggattgtata aacttacagg acccttccgt
     2821 agaaaaccat aggcttattg agactagttg gatagatgaa tacagcgcat ccttcagttc
     2881 cgaaatacaa atcctagcca gggacagaca gatgctcttg gccgatatca ccaaagctat
     2941 atcggatatg aagataaccg ttaccgcgat tagcgcgcgt accacaaaaa acaaacaggc
     3001 tataatcaat ataatgatgg aaattaatga cgtggaacaa ttaaaaagaa taattaagca
     3061 gtttaaacgg gtggatgagg tcatggatgt atttagggta aaagcatagg gggatttagg
     3121 gaagcaggat attttataac agacattctg cacttaagtc cgggggatgc gatacatcac
     3181 aattaataag ccgggaggat atatcgatta gatgcgaaat ccggcgacgt aactcatcct
     3241 atagcgaaag ccttttttaa tatacaggag tgattttatg agggcggttg tacaacgcgt
     3301 aagcacagcc agggttagag tggggcagga gataaccgga caaatagaca gaggcctttt
     3361 ggtttatttg ggcgtgggga tacaagatga ggcaagggat ttagactata tccttgacaa
     3421 ggttaccaaa ctcagagttt ttgaggacca agatggcaag atgaaccttt cagttgaaga
     3481 ggttggggga gccatactgg ttatatccca attcactctg tatggggatt gtagaagggg
     3541 taggagaccc agcttcaccg atgcggcggg tcctgataaa gccgaagatt tttataacca
     3601 atttatccat agaataaggg agatgggcat cagtgtagag acagggattt ttaaggccca
     3661 tatgcatgta gaatccctaa atgacggccc tgtcactatc ttactagata gcaggagaac
     3721 cttttagttt ttggaggaaa tcaactatga agataatagc tatgcctgtg ggtatattag
     3781 gtgctaattc atatattatt tatcacgaga taaccgggga tgccgttgtt atagaccccg
     3841 gcggtgacgg cgaaacaatc cttggggaga taagctccag ggagcttgag gttaagtata
     3901 tattgctgac ccatggacat tttgatcata tccatggggt agggtcgtta aaggaaagaa
     3961 cgggggctaa ggttgcgatt cacaaggagg atgcaggttc tctaggggat atccataaaa
     4021 acctttcctc ctttatgggg gttgaatcgg tgcagcctga agccgacata atgctggaac
     4081 atgggcaaag gcttgagttc ggggacatta ttctcaagat tatccatacc ccgggccatt
     4141 cacccgggag catatgctta aggttcgata atattgtttt taccggggac actctcttta
     4201 aaggttccat aggaaggacg gatttggtgg gtggggaatt gggacaaatt cttggttcca
     4261 tcaaggacag gcttttaacc ttagatgatg ggactattgt ttatcccggc catgggccta
     4321 tgaccacaat aggcgatgaa aagctgacca atcagttttt aaggggattg atttgatttg
     4381 accatatata ttttgacctc gatacaagaa tattccaatg agttggcgga attggctagg
     4441 gctttttatc ccggtagggt ggtaaaaacg gatactggca acccggagga tttattgcct
     4501 ggggatttgc tcttccgctg cgaatttcaa acggtggggg ataccaccaa aattgctgtg
     4561 aagctcttta aaggtatgga aacctccagt gttttttcta aaacccatag attaagacct
     4621 gatactactc ctatgaccaa gaggagggac attaagaact gtatgaagca gatgctctat
     4681 gatatattaa tggacctcac ggatcgtagc ttagattggg gggccctaac tggtgttaga
     4741 cccacaaaaa tagctcatgg gctcatagac gagggtctgg ataggaataa ggttttaaaa
     4801 ctcctatacg atgattacag ggtcagtcaa aagaaggcgg aactattgca cggcatagcc
     4861 aaaatccaac gtcccttctt ttgcggaaat cacccaaaca gaatttccgt atatataaac
     4921 atccccatat gccctaccaa atgcctttac tgttcattcc catcagccac catggatcaa
     4981 tgtgcgcatt tagtggacga ttatatcact gccttagaat atgagatgga aaccatgtcc
     5041 atattggcta gggaaaaggg gatagaggtt gaaacggtat atataggtgg gggcacacct
     5101 acttctttga atacggttca gttggaaagg gtcttacggg cagttcggga attttgggga
     5161 tggagtccat ggaaggaata caccgtagaa ggcggaaggc ccgataccat gtctggggat
     5221 aagctgaggg tgttaaagga tcatagtgtc actcgcatca gcataaaccc ccagagtatg
     5281 aacgataaaa ctctgggagc aatcgggaga aatcataagg cccatgagat cgtcgaatgc
     5341 ttttatagag ccagagatgt agggttcgat tgcataaata tggatgtaat agtgggtctg
     5401 ccgggcgagg ggatagagga agggcgccgt acaatgggaa gcataaaaaa gctcgccccc
     5461 gataatctca ccgtccatac tctggccgta aaaagggcat caaggcttaa ggacaatttg
     5521 gaacgatttg cacttaccga cgagagactt gcggctggaa tgatggacat cttttatgag
     5581 ggtgccggat ctatggggat gaaaccctat tacctgtatc gccaaaaata tatgttgggg
     5641 aatatggaga atgtagggtt tgctacaccg ggaaaagaat ccatctataa catgcagatt
     5701 atggaagatc ggcaaacgat ctgggcattc ggagcagcag ctattaccaa ggtgatttat
     5761 cctaccatgg gacgcataga acgggctgcc aacgttaaga acatcggtga ttatattgat
     5821 aggacagaag agatggtgca aagaaagctg aaaatatgca cttattcctg aagaggaggt
     5881 aaagtatgaa tataaaggca ccaaggggaa ctaaggacgt attgccggat gagtcctata
     5941 gatggcacta tttagagggg attattcggg aaataactca attgtttgga tacaaggaaa
     6001 taagaacccc ggtatttgag cataccgagc tgtttgtgcg gggtgtaggg gatacgacag
     6061 atatagtaca gaaggaaatg tatacctttt tagataaggg cgacaggagt ataaccttaa
     6121 agcccgaggg tacagcgggg gccgtacgcg cgtatataga aaacagtctc tatggaatgg
     6181 cgcaacccgt caagatgtat tatttaaccc cggtttttag gtacgaaaat ccccaggcgg
     6241 ggagactcag ggagcatcat cagtttggag ttgaagtatt tggggccaag gaaccgacta
     6301 tagatgcgga aataattatg ttggctacta agctctttga aaggttagga ctcagtggac
     6361 tggaactcaa tataaacagc atcggatgtc cccaatgtcg ccctggctac catgatgccc
     6421 ttagggaata cctgaagggt catcttgggg aattatgcca tacatgtaat tcgcgatatg
     6481 agagaaatcc ccttcggata ctggattgca aaacagaaaa atgcagtgcc atagtaaagg
     6541 atgctccgat tattttagat tacctatgta aggaatgcag cgcccacttt aacgggctac
     6601 aggattatct caaggcggct tccgtggaat ataatataaa cccaatgata gtaaggggat
     6661 tggattatta cagcaagact gtatttgaga taatctctac agaaataggt gcccagggga
     6721 cagtgtgcgg cgggggcagg tatgatcccc tggtcaagga atgcggaggt ccggatactc
     6781 ccggagccgg atttggtctg gggctggaga gattattact agccttagac ggacagggac
     6841 tggagatacc taaggctccg ggaagggatg ttttttttgt gaccataggt gaggctgcca
     6901 gagacaaggc cttcgaactt ctggatacca tgagaaatga ggggatatta gccgacatgg
     6961 atcatgtggg aaggggagtg aaggcccagt ttaagtatgc agacaaggca gatgttacct
     7021 gggtatgcat attaggggac gatgaactca gcaggggcga ggttaaggta cgggccatgg
     7081 ccgacggcag ggaggaaact gtatcccaag ccaatctggc tgaatatttt aagagcaaaa
     7141 aggaaaggtg aaaagagatg ggtgaatcta taagtgggct aaagaggact cacatgtgtg
     7201 gggaacttac atccaatgat ctgggccaaa atgtggttct gatgggatgg gtacaaagac
     7261 gaagggattt aggggggttg atttttgtta ccctcaggga taaaaccggg ataatccagc
     7321 tggtatttga ccaggacgaa tccaaggccc tgttccataa ggcggccagg ctacgcagcg
     7381 aatatgttat tgcgtccgca ggcgaggtta tagccagggc acctgaggcc ataaaccccg
     7441 atatgcctac aggcagtatt gaaataaagg ttaaagagct aagaatttta tccggggctg
     7501 agaccccgcc tttttatatt gaagacaaca tcaacgttgg ggaggcgcta aggtatagat
     7561 atagatactt agaccttaga agaccggaga tgcaaaacaa ccttttggta agacacagga
     7621 ttgccaactc cgtcagggat tttctcaacg ggaaggggtt catggagatt gaaaccccca
     7681 tgctgaccaa aagcacgccg gaaggtgcca gggattatct cgtgccaagc agggttcatc
     7741 tcgggaaatt ctatgcccta ccccagtcac cccagctctt taagcagctc ttgatgctgg
     7801 ccggctatga cagatactat caactggcaa ggtgcttcag ggatgaggat ttgcgggctg
     7861 ataggcaacc ggaattcacc cagatcgaca ttgaaatgtc ctttgtagag atagaggata
     7921 tattggctat taatgaggaa atgattgccg acgtattcag acgagtgctg ggcatcgaga
     7981 tttcactacc cctcaaacgg ataacctata aggaggcctt ggaaaggtat ggccttgata
     8041 agcccgatac aaggtttggg ctggaactta tggatgtcag tgctttggtg gctgattcgg
     8101 actttaaggt attctccaat gcggttgggt ccgggggcag cgtcagggct atcaatgtaa
     8161 agggatgcgg aaacaaattt tctcggaggg aaatagattc tttgggcgaa tatgtaaagg
     8221 attatagggc aaaaggcctg gcctggatac attacagcga ggatgggata aggtcgccga
     8281 tagctaaatt tttagcccgg gaagaaatgg atgcaattat aaaatacatg gatgctgagg
     8341 ttggggatct tctattgttc gttgcagata gggatgaaat tgttttccag gctttgggaa
     8401 atctgagaca gaaactggga agacagctgg atttgatacc aactgatcga tacgacctcc
     8461 tgtggattac agagtttcca ctattcgaat atgacgaggg ggaaaaccgc tttgtcgcga
     8521 aacaccatcc cttcacctca cctatggatg aggatataga tctactggat acacggcctg
     8581 gggagactag ggcgaaggct tatgatatgg ttttaaacgg caatgagata ggcggcggca
     8641 gtataaggat tcatgatata gaggttcagg aaaggatgtt caaggcattg gggttcaccc
     8701 tagaagaggc gtgggaacag tttgggttct tgatggaggc tttcaagtac ggaacaccgc
     8761 cccatggggg aattgcttat ggactggata gaattgctgc cctccttgcc ggaagaccta
     8821 ccatcagaga tgttattgcg tttcccaaag tccagaatgc gtcggacccc atgaccaacg
     8881 ctcccacagc ggtagattca aggcaattgg aggatttgca tattgcaatt gcaaaaaatg
     8941 gtgaggataa ggatgtttaa tatattttta attggttaac tataaactta tagtg
//
LOCUS       DASRNX010000111         8643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_235999,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000111 DASRNX010000000
VERSION     DASRNX010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8643
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235999"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..364)
                     /locus_tag="VFD89_04355"
     CDS             complement(191..364)
                     /locus_tag="VFD89_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HZJ57453.1"
                     /translation="MEKHVSRKNHPLEGVKCTVDSCYYNSGGIRCNAASIEIQPPGAI
                     DTEETDCVTFVSK"
     gene            707..2182
                     /locus_tag="VFD89_04360"
     CDS             707..2182
                     /locus_tag="VFD89_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZJ57454.1"
                     /translation="MYLYFIIVYVVVIALIGLVSMARVKGISDFFLAGRSINPWMSAF
                     SYGSAYFSAVLFIGYAGKIGWNFGISALWVVLGNTFVGSFLAWKVLGARTREMTNRLN
                     ATTMPEFIGIRYQSKNLRIATALIIFVFLIPYSASVYKGLGFLFEQVFKIPSGIILLI
                     IAFFTAFYLVLGGYVASAMADFVQGLIMIIGVILMLFYIISHPKVGGVSNVLSSLGKI
                     DVGLIKPVGPQGLLTTFSLVILTSLGSWGLPQMVHKFYTIRDEKSIDAAKWVSTAFAL
                     IITFGAYYTGIVSRLFYPESMPVFNGAANADVLIPNVLTQTLPEAVLGVILILVLSAS
                     MSTLASVILASSSAISLDLIRGVFCPNIDNKKLTLLTRFLCLLFVILSFVIAILPNPV
                     MILASISWGAVSGCLLAPYLYGLFWKRTTKAGVWAGIVTACCIIIGGAIFTKMDETWM
                     PILSAMSIIIPLLVVPLVSLVTEPYSKEHTEMTYNFSQRAS"
     gene            2359..4083
                     /gene="iorA"
                     /locus_tag="VFD89_04365"
     CDS             2359..4083
                     /gene="iorA"
                     /locus_tag="VFD89_04365"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HZJ57455.1"
                     /translation="MELMLGNKAVARGAYESGVRVATAYPGTPSTEITEFIAKYDEIY
                     TEWSPNEKIAMEVAIGASFAGGRAMVSMKHVGLNVAADPLFTVSYTGVNGGLVIVVAD
                     DPGMHSSQNEQDSRYYARSAHIPMLEPADSQESKDYIKLAFELSEKYDTPVMVRLTTR
                     ISHSQSMVSVAESLPVRIREYTKNPQKNVMMPAMARARHVFVEQRMKDLSEDSSKFDI
                     NEIEMGDKQVGIIASGIVYQYAKEAFPGASFLKLGMVHPLPKDLILDFASRVNTLYVL
                     EELEPIIEEQIKSWGIRVIGKELFSVQGEYTPNMIEQAIKATFIDTRPPEILPNRPPV
                     LCPGCPHRAVFHTLKRLRLTATGDIGCYTLGALSPLEGLDTCVCMGASIGMAHGFEKV
                     RGKDFAKKMVGIIGDSTFIHSGIGGLIDIVYNKGMSTVLILDNSITAMTGHQDNPSTG
                     RTIKGEEAPVLDLVTLVKAVGINNIHVIDPFDLEVSRKVLKMELEREEPSVIIFKRPC
                     ALLDKSRSHPLRIVPERCTDCGLCLRIGCPAIQRLDEGMIIDDSLCNGCGLCERICRF
                     NAMERVDI"
     gene            4084..4662
                     /locus_tag="VFD89_04370"
     CDS             4084..4662
                     /locus_tag="VFD89_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HZJ57456.1"
                     /translation="MQSINILIVGVGGQGTLLTSRVLGNLAGGLGYDVKVSEVHGMAQ
                     RGGSVVTHVRFGDRVYSPLVEVGKADVLLSFEKMEALRWLHFLKEEGMLIVNDQSINP
                     MSVVTGAAEYPDDILDRLKKSTAGVTIINALDIAKGLGNIRVTNSVLLGVLAKNMDIE
                     YEEWKGALSRTVPPRTVEINLKAFDAGYNIEV"
     gene            4681..5979
                     /locus_tag="VFD89_04375"
     CDS             4681..5979
                     /locus_tag="VFD89_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HZJ57457.1"
                     /translation="MNYWNKFCECMGRRELDKLQSERLVETIERVYYNVPYYRNLMQE
                     RGLMPEDIKGVDDLNRLPFTTKDDLRENYPYGLFAVPLSEIVRIHASSGTTGKPTVVG
                     YTRRDIAIWSELMARTLTCGGATKESVIQVAYGYGLFTGGLGVHYGAERLGASVIPIS
                     GGNTKRQIMLMKDFGTTVLACTPSYALYIAETMSEMGLEKGDFSLKYGILGAEPWTEE
                     LRREIEEKLGITAIDIYGLSEIIGPGVASECLYKTGLHICEDHFIPEIIDPATGQVLP
                     EGSEGELVFTTITKEGIPVIRYRTRDITSLNYEPCRCGRTHARMHKVIGRTDDMLIIR
                     GVNVFPSQIESILIDIGETEPHYLLVIDRVGNMDNLEVWVEVSEKLFSDEVRRLEELN
                     RRIAHEIQSILGLTVRVKLVEPKTIERSQGKAVRVIDKRK"
     gene            5993..6424
                     /locus_tag="VFD89_04380"
     CDS             5993..6424
                     /locus_tag="VFD89_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZJ57458.1"
                     /translation="MKVKQISVFLENKKGRLAQLTGILKENNIDISAISVADTANFGI
                     IRMIVSRCDEAIRVIKDAGFTVNTADVLAIEVSDSPGGLHQVLDTLSKNDIEIEYLYS
                     FVRRPSERALILFRIRKIEEAARILGHHNIKVLSPEEIYGL"
     gene            6445..7578
                     /gene="alr"
                     /locus_tag="VFD89_04385"
     CDS             6445..7578
                     /gene="alr"
                     /locus_tag="VFD89_04385"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZJ57459.1"
                     /translation="MRPTRAEIDLTKLGDNIKAIKSKLNPKTAVMAIVKANAYGHGIL
                     EVANESLKSGALSLGVAIPEEGIKLRYGGIDCDILVLGSIVADQMNLVLEYDLSLCLF
                     SMETAVLVNELAGRKRKKAKVHLKVDTGMGRIGIRDRDEAVKLGIAIDQMDHLIFEGV
                     FTHLSSADESNQDYSAIQLGRFVDVIDDIRRYGVHPTWVHAANTASIVNLPKSHFNMV
                     RAGIGIYGYLPSHILGSGKDVKVDPILSWHTKVVHIKPIGTGCAISYDRAYIAKEPRV
                     VATLPVGYGDGYSRLLGNKGWVLIRGKIAPIIGNICMDQMMVDITDIPGTEVGDDVVL
                     IGEQGNRRITADDMARLYGTISYEVLTNISGRVPRIYKGDENG"
     gene            7571..8152
                     /locus_tag="VFD89_04390"
     CDS             7571..8152
                     /locus_tag="VFD89_04390"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZJ57460.1"
                     /translation="MDNVSERKRLIRKEMLSLRRALSMENYIKTNNDIQTLFNSMAFN
                     RRALNYMSYVNIGKEVNTRNIIERLLLDGKNVSVPVCIPETTRLKASQIYDFSDLESS
                     QFGLLEPKSGLIREVNPKDIEIVLVPGLAFDRKGNRLGYGKGYYDGFLIELTPGALKI
                     GLSYSFQIIEEVPVDDFDIPLDIIVTEKEVIQV"
     gene            8557..>8643
                     /locus_tag="VFD89_04395"
     CDS             8557..>8643
                     /locus_tag="VFD89_04395"
                     /inference="COORDINATES: protein motif:HMM:NF017221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57461.1"
                     /translation="MDEIVQKTIDAIDEGKKEIFEIAEDSRRE"
BASE COUNT         2471 a         1612 c         2141 g         2419 t
ORIGIN      
        1 ctgactctta atcagggcgt cccgggttcg aatccctgat ggcccaccaa gcagaatccc
       61 cggaaacatt ggtttccggg gattctgctt catcttgcag tttacatttc catgtcttaa
      121 tcaaaagaca ttctccgata atacatccta ttatttccgc catttaggct atggggagca
      181 aattagagtt ttatttactg acaaaggtta cgcaatccgt ttcttctgta tcaattgctc
      241 ccgggggctg tatttcgatg cttgcggcat tacagcgtat tccgccactg ttgtaatagc
      301 aactatccac agtacacttt acaccttcta acggatgatt ttttcgggac acatgttttt
      361 ccattatata attcctccta aaataaaacc atattttctg agacgtcgta tattttgatg
      421 cgcatactct ctaatggcat ccaggtattt tgtagaattg cctatataca attcatagac
      481 ttattttatc ctaattaagc gcctccctat tctatgtaaa gaaatcctta tagaccaaac
      541 gacagaatgc taattatcct tcgaaagtta aaaataagca atggggtttc ttttttctca
      601 aaaataccgt ataatcatcc atatggaagc atagcagttt gggaaaaatg tttcagttta
      661 agattttaat aaacaattat atggggtagt acaggaggat ggtagcatgt atctatattt
      721 cattattgta tacgtagttg taattgcact aattggactg gtgagtatgg ccagggtaaa
      781 aggtatatcc gatttttttc ttgcgggacg atcaataaac ccatggatgt cagccttttc
      841 ctacggcagt gcatactttt ccgcggttct attcattgga tatgcgggta agataggttg
      901 gaattttggg atttccgctt tgtgggtagt tttgggcaat acctttgtag gcagtttttt
      961 agcttggaag gtacttggtg cccggacgag ggagatgacc aatcgtctta atgcaacgac
     1021 aatgccggag tttataggga taaggtatca aagcaagaat ttaaggatcg ccaccgctct
     1081 tataatattt gtgttcctaa tcccatattc tgcctcggta tataaaggac tgggttttct
     1141 ttttgagcag gtatttaaaa taccaagtgg aattatccta ttgataatcg cgttctttac
     1201 ggcgttttat cttgtcctag gtggctatgt tgcgtctgcc atggctgatt ttgttcaggg
     1261 acttattatg ataataggtg tcatattgat gctgttttat attatctcgc atccgaaggt
     1321 ggggggggtt tccaatgtct tatcgtcact ggggaaaatc gatgtagggc tgataaagcc
     1381 tgtaggaccc cagggtcttt tgaccacttt ttcattggtc atattaacca gccttggcag
     1441 ttggggattg ccccaaatgg tccacaagtt ctataccata agggacgaaa aatccataga
     1501 tgcggcaaag tgggtgtcaa cggcttttgc tcttataata acctttgggg cctattatac
     1561 ggggatagtc agcagattat tttatccaga atcaatgccc gtgtttaacg gtgcagctaa
     1621 cgctgatgtg ttgataccca acgttttgac ccaaacccta cccgaagcgg tgttaggtgt
     1681 cattttaata ttagtgcttt cggcctctat gtcaacgctg gcttcggtaa tattggcatc
     1741 gagttctgca atttctttag accttatacg aggtgttttt tgccccaata ttgacaataa
     1801 gaagcttaca cttttaacga ggtttctatg tctactattt gtgattctct ctttcgttat
     1861 tgctatttta cctaacccgg ttatgatctt ggcctcaatc tcatggggtg cggtttccgg
     1921 ttgtctttta gccccatatc tgtatggatt gttctggaaa agaacaacaa aagcaggagt
     1981 atgggccggt atagtcaccg cttgttgcat cataataggg ggagctatat ttaccaagat
     2041 ggatgagaca tggatgccca tactcagtgc tatgtcaatt attatccccc tccttgtggt
     2101 cccccttgtg agcctggtca ctgaaccata tagcaaggag catacagaaa tgacatataa
     2161 ctttagtcaa agagctagtt aaagtcccgg ggtaaattgt aggttctcta tcagctgtat
     2221 tttttagatc ggcgggggcc agatttaact ggggaaacct tccacgggat tatgagatcg
     2281 accaataaaa tgttatcata atcggactaa cacttggaga taaaattcat ttatttttca
     2341 tcacggaggt attctactat ggaattgatg ttaggaaata aagccgttgc cagaggggca
     2401 tacgaatccg gggttagggt agcaacagca tatcccggaa cccccagcac agagattacc
     2461 gaattcatcg caaaatacga tgaaatatat accgaatggt cgcccaatga aaagattgcc
     2521 atggaagttg ccataggggc ctcttttgcc ggcgggcggg caatggtgtc tatgaagcat
     2581 gtggggctga atgttgctgc cgatccgctg tttaccgtat catataccgg agtgaacggt
     2641 ggcttggtta ttgtagtggc cgatgatccg ggtatgcata gctctcagaa cgaacaagat
     2701 agcagatatt atgcaagatc agcccatatc ccaatgctgg agcccgcaga cagtcaagaa
     2761 tcaaaggatt atataaagct cgcctttgaa ctaagcgaga aatatgacac ccctgttatg
     2821 gtgagattga ctaccaggat atcacactct caaagcatgg ttagtgtagc cgaatccttg
     2881 ccggttcgga taagggaata tacaaaaaat ccgcaaaaaa atgttatgat gccggcaatg
     2941 gcaagagccc ggcacgtatt tgtagaacaa agaatgaagg acctatccga ggattccagc
     3001 aagtttgata ttaatgaaat agagatgggc gacaagcagg tcggaattat agcgagtgga
     3061 attgtttacc aatatgcaaa agaagcattt cccggagcat ccttcctaaa gttgggtatg
     3121 gttcaccccc tgccaaagga cctaattttg gatttcgcct cccgcgtaaa tactctgtat
     3181 gtcctggaag aattggaacc aataattgaa gagcagataa agagttgggg gattcgggtc
     3241 ataggtaaag aattgttttc agttcaggga gaatatacac ccaatatgat tgagcaggcg
     3301 attaaggcta ctttcattga tacccggcct cccgaaatat tacctaacag gcctccggtc
     3361 ttatgcccgg gttgtcccca tagggcggta ttccacaccc tcaagagact caggctgacg
     3421 gcgacggggg atatagggtg ctataccctg ggggcgctgt ctcccctcga aggattggat
     3481 acatgtgttt gcatgggagc cagtataggt atggcccacg gttttgaaaa ggtccggggt
     3541 aaggattttg caaaaaagat ggtaggcata atcggagatt ccaccttcat acattccggg
     3601 ataggcggtc tcatcgatat cgtatataat aagggtatgt caaccgtttt gattttggac
     3661 aattccataa cagccatgac agggcatcaa gacaacccgt ccaccgggcg tactataaag
     3721 ggtgaagaag cccctgtatt ggatttggta acccttgtca aggcggtggg tataaataat
     3781 atccatgtca tagacccttt tgatttggaa gtttcgagaa aggtactgaa gatggagttg
     3841 gaaagggaag agccatctgt aattatcttt aaaaggccct gtgcgttgct agataaatcc
     3901 agatctcacc cccttaggat tgtcccagaa aggtgtaccg attgcgggct atgtcttagg
     3961 ataggatgtc cagctattca aaggttggat gagggtatga ttatcgatga ttccctatgt
     4021 aacggttgcg ggctatgtga gaggatatgc aggttcaacg ctatggaaag ggtggacata
     4081 taaatgcaga gtattaatat actgattgtt ggggttggtg gacaaggcac cttgttgacc
     4141 agtagggttt taggcaacct cgccggtgga ttaggatatg atgtaaaggt ctccgaagtc
     4201 catgggatgg cccaaagagg gggtagcgtt gtaacccacg taaggtttgg tgatagggtt
     4261 tattcccccc tggtagaggt ggggaaggcg gatgtattgc tgtcctttga gaaaatggaa
     4321 gcattaagat ggttgcactt cctaaaagag gagggtatgc tgatagttaa cgaccaatcc
     4381 ataaatccta tgtcggttgt tacaggtgca gctgagtatc cggatgatat cttggataga
     4441 cttaaaaaat ccaccgccgg tgttactata atcaatgcgt tggacatagc caaggggctt
     4501 gggaatattc gtgtaactaa ttcggtgtta ttgggtgttt tggcaaagaa tatggatata
     4561 gagtatgaag agtggaaagg ggctttaagc cgtactgtac cgccccgaac cgttgaaatt
     4621 aatcttaaag ccttcgatgc tggatataat atcgaggtat aggggaggta ttcaattcaa
     4681 atgaattatt ggaacaagtt ttgtgaatgc atggggcgta gggaactgga taagctgcaa
     4741 agcgaacggc ttgttgagac gatagaaagg gtatattata atgtccccta ctatcggaac
     4801 ctaatgcaag agaggggtct tatgcctgaa gatatcaagg gagtggatga tctcaacaga
     4861 ctgcccttta cgacaaaaga cgatcttcgg gaaaattatc cctatgggct ctttgctgtg
     4921 cccctcagtg agatagtgag aattcatgcc tcatccggca ctaccggaaa gcccacggtt
     4981 gtgggatata cccgtcggga tatagctatt tggtcggaac tcatggccag aacgttgacc
     5041 tgtggggggg cgactaagga atctgtgatt caagtagcct atggttatgg tctattcacc
     5101 ggtgggctgg gcgttcatta tggagccgag cgtctggggg cttccgtaat tcccatatcc
     5161 gggggtaata caaagaggca aataatgctt atgaaggact tcggaaccac ggtcttggcc
     5221 tgcactccgt cctatgcatt gtatatagcg gagaccatgt ccgaaatggg gctggaaaaa
     5281 ggagatttta gtttaaaata cggtatctta ggggcagaac cgtggacgga agagttacgc
     5341 cgggaaatag aagaaaaatt ggggattacc gccatagaca tatatgggct cagtgaaata
     5401 ataggtcccg gagtcgccag cgaatgcctg tacaaaaccg gcctccatat ttgcgaggat
     5461 cattttatcc ccgaaattat tgatccggct accggtcagg tattgcctga aggctctgaa
     5521 ggtgaattgg ttttcaccac tataaccaag gaaggaattc cggttatcag atatcgcacc
     5581 agggatatca cctcattgaa ttatgaaccc tgccgatgcg ggaggaccca tgcaaggatg
     5641 cataaggtta taggaagaac cgatgatatg ctcattatcc gaggggttaa tgtgtttccg
     5701 tctcagattg aaagcatcct gatagacatc ggagaaaccg aaccccatta tcttctggtt
     5761 atagaccggg tagggaatat ggacaatttg gaagtctggg tagaggtgtc tgaaaaatta
     5821 ttttccgatg aggtccgtag gttggaagaa ttaaatcggc ggattgccca tgagatacag
     5881 agcattctgg gccttacggt aagagtgaaa ttagtagagc cgaaaaccat tgaaagaagc
     5941 caaggtaagg ctgtacgggt tattgataag aggaaataaa aaggagggca aaatgaaggt
     6001 aaaacagata tcggtatttc ttgaaaataa gaaagggcgg ttggcccagc tcaccggcat
     6061 attaaaggaa aacaatatcg atataagcgc tatctctgta gccgataccg ctaatttcgg
     6121 tataattagg atgatagtca gccgatgtga tgaggccatc cgggtgataa aggatgcagg
     6181 atttacagta aatactgccg atgtattggc catagaggtg tcggattcgc ccggcggatt
     6241 gcatcaggta ctggataccc ttagcaaaaa cgatattgaa atcgagtacc tgtactcttt
     6301 tgtgcggagg ccttcggaaa gggccttgat cctctttaga atccggaaga ttgaggaggc
     6361 cgcgaggata ttgggccatc acaatatcaa agtattatcc ccggaggaga tatatggatt
     6421 ataattacca aagaggtgac ttttgtgaga cctaccaggg ctgaaattga tcttacaaag
     6481 ctgggagaca atataaaagc aataaagagc aaattgaatc ccaaaaccgc agttatggct
     6541 attgttaaag ccaatgccta tggccacggt attttggaag ttgctaatga atcccttaaa
     6601 tccggagctc tctccttagg ggtggcaatc cccgaagaag gaataaagct gcggtatggg
     6661 ggtatcgatt gcgatatttt ggtactgggg agcattgttg cagatcaaat gaatttagtg
     6721 ctggaatatg acctttccct gtgcctgttt tctatggaga cggccgtact tgttaatgaa
     6781 ttggccggaa gaaaacgcaa aaaggcaaag gtccatctta aagttgacac aggaatgggg
     6841 agaattggaa taagggacag ggatgaagcc gtaaagctcg gcatcgctat agatcaaatg
     6901 gatcatttga tctttgaggg ggttttcacc catctatcta gtgctgacga atccaaccag
     6961 gactattcag ctattcaatt aggccggttt gtggacgtca tcgatgatat acgtcgctat
     7021 ggagtacacc caacatgggt acatgccgca aatacggcat caatagtcaa cctcccgaaa
     7081 tcccatttta atatggtaag ggcgggaatt ggaatatatg gctatctgcc aagtcatatt
     7141 ttaggatctg ggaaggatgt aaaagtagat ccgattctaa gctggcatac taaggtggtt
     7201 catataaagc ccattggtac gggttgtgca ataagctatg acagggctta tatcgctaaa
     7261 gagcctaggg ttgtagctac cctcccggtg gggtatggtg atggctatag ccgcttattg
     7321 ggcaacaagg ggtgggtttt gattagggga aagattgccc cgattatcgg taatatttgc
     7381 atggatcaga tgatggtgga tataactgat atccccggta cggaagtagg cgatgatgtg
     7441 gtattgattg gcgaacaggg aaatagaagg atcacagccg acgatatggc caggttatat
     7501 gggactatat cctacgaagt tctcaccaat ataagcggca gagtgccccg aatttataag
     7561 ggggatgaaa atggataatg tgagtgaacg gaaaagacta attcgcaagg aaatgctgag
     7621 cctgagaagg gcactatcaa tggagaacta tataaaaacc aataacgaca tacagaccct
     7681 atttaattct atggcattta accgcagggc gctaaactat atgagctatg tcaatatagg
     7741 caaggaggtt aataccagga acataattga gcgtctgctt ttggacggaa aaaatgtttc
     7801 ggtcccagta tgtattcctg agactacccg gcttaaggct tcccaaatct atgattttag
     7861 tgatttggaa tcgtctcaat ttggtctgtt ggagccaaaa tcgggattaa tcagggaggt
     7921 aaaccccaag gacatagaaa tagtcctggt acccgggttg gcctttgaca gaaagggcaa
     7981 taggttagga tatggtaaag gatattatga cggttttcta atagagttga cacccggagc
     8041 attaaagatt ggactgtcat atagttttca aattatagaa gaagtccctg tagacgattt
     8101 tgatataccc cttgacatta tcgtgactga aaaggaagtc atacaggttt aaaacgccaa
     8161 gggcatgctt ggttctttaa attggttcag tctgttaatg gccaatttca actacaaaat
     8221 ttatcatcat gaactgcata gacccattgt aattactatg ccgataaatt tagttttctt
     8281 atttcttatc tataatttat tgtaccacaa aaatagctat tcaaatttca ttttgaatta
     8341 aagaggatat tgggtattta tgtcgaatta taacgttagc attgcattta tattcttcgg
     8401 aacgcttgaa tcagcgctta ggcttgtata tagtcaccat tagccaaaca gcttttaccc
     8461 tatccatatc aattttagta atgtgcttgg atgaataaat tatcgtttaa ataggagtgt
     8521 aatatgggtt caaataattc tatcagattg gttcaaatgg acgaaattgt ccagaaaaca
     8581 atagatgcta tcgacgaggg aaagaaggag atcttcgaga tagccgaaga ttcaaggcgt
     8641 gag
//
LOCUS       DASRNX010000112        23734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_659047,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000112 DASRNX010000000
VERSION     DASRNX010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 23734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23734
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659047"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1493)
                     /locus_tag="VFD89_04400"
     CDS             complement(165..1493)
                     /locus_tag="VFD89_04400"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ57462.1"
                     /translation="MMINKQKIDAGAFMEELREIIMASRGLILFKELSELPALRRLTQ
                     LLEGIAENSWSPAQTAEGYSGFLNAFLSSIPTHAPGEYSWKDWVIDAVLHTENRFTLW
                     AETRKGYIPRAMTAAAENDLPSIQKLANIPWQDLADLLEGEIKGFRPVNIFHCRQTPN
                     WQEVCARRGLAGWNVNALIDYYSLKGSGLFSRYRGFYWDGTALKGIKNTDPITMDRLV
                     GYDIQKRIILDNTEKFISGYSANNVLLYGDKGTGKSSMVKALIHRYWDRGLRIIELPK
                     IHLDDYYSILRHIEDRMFRFILFIDDLSFEEHEVEYKHIKALLEGGLKVRPSNLLVYA
                     TSNRRHLVREVVADRSSTGYQYNDRNEISPTDSMQEKLSLADRFGITLTFISPNQRGY
                     LEMVESMAEERGIDIGREALHAEALRWEKRYNGRSGRTAKQFIDHLEGGL"
     gene            complement(1701..3008)
                     /locus_tag="VFD89_04405"
     CDS             complement(1701..3008)
                     /locus_tag="VFD89_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZJ57463.1"
                     /translation="MKTLIKNINILTVDENSNQYQSGCILIDGHEITFVGYERDLGVA
                     AGTDIDIVEDGRGMLAIPGLVNSHTHTAMTLFRGYANDLPLMKWLEEKIWPAEDRLAQ
                     DDVYWLSLLAISEMIKGGVTAFADMYMFMEQTALSVLHSGIRGVLARGLQGPDQKSRD
                     RLEENRILWENWHGEGDGRIRVMVGPHGVYTCEPSYLAEVLELAHSLGTGIHIHLSET
                     RQEVENCIKEYGKSPVMHLNDLGLLGGNTLAAHCVHLSSEDLDVLKDRDVKIVHCPIS
                     NLKLGSGIAPVHEMLSKGIAVSLGTDGAASNNNLSIMKEMAFAALISKGVKEDATLIS
                     AATALKMATNHGAGALGLEDEIGSISPGKKADIVLVDTGEPHYHPHGDWTTHLVYSGH
                     SGDVDSVWVNGKRLMKNKALLTIDSDKVYSKVRSIYKRVMGSR"
     gene            3233..3532
                     /locus_tag="VFD89_04410"
     CDS             3233..3532
                     /locus_tag="VFD89_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57464.1"
                     /translation="MPTPQYVKEFNADLGELSLNTGAGKHWKRFAANNIAAIRKTTVE
                     RKKLFITRTVDRLEIDINGEEVPYIIEKGVIRDDFEWVADVLKSFAEKNKVDYHE"
     gene            complement(3558..4700)
                     /locus_tag="VFD89_04415"
     CDS             complement(3558..4700)
                     /locus_tag="VFD89_04415"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HZJ57465.1"
                     /translation="MAYKILIADDMLVNRRLIKRALEQRIEDADFHEAEDGEKALRYI
                     FKNRVDIIILDLIMPNKDGFQVLKELKSSEVYRDIPIIVNSAVDEVGVIQKALELGAT
                     DYFTKPLTMEQMGVVLPVKVKNALKAYEQKRLLVQMNQRMREELEFANALQSILIDES
                     KSLPGIEMIGRYIPSAELSGDYYECLQTGDRTWLLIADVSGHGVASAMISSMVKVMFK
                     NLVDRYHAPAQVLESMNETFYNLTHGTYYITAFLGLIEDGVLTYCNGGHPYPVILDSK
                     ENRTYMLEENGLILGVMEDYRYRTYSRRISPGDYIFMYTDGLLEPRIAGRDMKTCNDF
                     LDYIEKHKDMIREDPALLLDSIVKDFGKDSPEGLRDDVAVIIIRKK"
     gene            complement(4713..5099)
                     /locus_tag="VFD89_04420"
     CDS             complement(4713..5099)
                     /locus_tag="VFD89_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZJ57466.1"
                     /translation="MNTIVGNESAIKRILLVDDSPIIHRLLRKTLEAHGYEVCGDAKN
                     GREGVSLYQSLKPDLVFMDITMPVMDGLEAAEEIMGIDPEARIIMLSAMGDEQIVNRA
                     KALGIEDFLKKPFNDYMIISAISGIV"
     gene            complement(5165..5626)
                     /locus_tag="VFD89_04425"
     CDS             complement(5165..5626)
                     /locus_tag="VFD89_04425"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HZJ57467.1"
                     /translation="MDIKYILPFLEALKGILEQFGATDIKRGNMTKNDLMHVDTDITA
                     VVGMVGRVRGNIAYSMATDTAKRIVSAMMMGMPVEELNEMGRSALGEFSNMVTGNAST
                     LLSGSGIQVDITPPSIIFGRDVYFIISSVETLKIDMESSLGRVEVNIGLEL"
     gene            complement(5626..6102)
                     /locus_tag="VFD89_04430"
     CDS             complement(5626..6102)
                     /locus_tag="VFD89_04430"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HZJ57468.1"
                     /translation="MDERLYNPFCISTLDIIKEMSDIDISGMDSLKEEAHELTSMGIS
                     SIITFAGAKKGRLLIDMEPSLALSLAGTILGEEYDDIKDPMVLSLISEVNNIISGNAV
                     TGLNDDFLMDLRLAPPVVFAGSDVVISIPKLRSFSSHGKTDFGMIRINIAWEGGSS"
     gene            complement(6148..7005)
                     /locus_tag="VFD89_04435"
     CDS             complement(6148..7005)
                     /locus_tag="VFD89_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HZJ57469.1"
                     /translation="MRDLTAREFELMRGYIEKECGIVLGKDKAYLITSRLSRILTEFN
                     IHSFEGLYFTLKSEKDPRIKEKVIDAITTNETLWFRDRTPFKTLEEVLLPRYIDEIQN
                     KKRDRVRIWSAGCSTGQEPYSIAMSIDRYLTQRRIQGVSPKDFEILATDISRSVLKAA
                     EAGRYDDVSIMRGLDLDYRNEYFTRESRDWVLHNRIRDRVRFRQFNLQNNFITLGKFD
                     VIFCRYVTIYFSPDLKRDIFKKIAGALNLPCGVLFLGNSEVFNEYGNNFVRMSHDGGV
                     YYKVKEPVL"
     gene            complement(7005..8177)
                     /gene="cheB"
                     /locus_tag="VFD89_04440"
     CDS             complement(7005..8177)
                     /gene="cheB"
                     /locus_tag="VFD89_04440"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HZJ57470.1"
                     /translation="MDKLKVLVVDDSLVYRKIISRAVERTDKAVVDCTASSGITALER
                     LEQRPIDVVLMDVFMPGMDGLETLTRIKDKYGDLPVVIISSGGVDGAKLTVRALEYGA
                     MDFIQKPSQGHGNKNIDAISRHLNILFSQILIDRYAINNRENQVSKDAIPKAGRRLDA
                     SRAREGESLTRGSGIIPGDGKRPPRADRASGYGTPCLSGIDLVVIASSTGGPNAVEEV
                     CQGIGPGFTKPVLIVQHMPPDFTKIFSRFLGRKCEMPVKEAEEGDKIVGGQVLVAPGG
                     YHMSVNARGPQITLTTSPPVNGVRPAADVLFESVAEAYAGKRVLAVILTGMGSDGTRG
                     VKALKEACQCYCITQSRETCVIYGMPRSVFEAGLSDEVVDLKDIGRRIIEINRGRC"
     gene            complement(8202..10583)
                     /locus_tag="VFD89_04445"
     CDS             complement(8202..10583)
                     /locus_tag="VFD89_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZJ57471.1"
                     /translation="MRIRSKLLLSFSAICAFALVIGLVGYWGMTGVMKELDDMGEAEL
                     PKVKNILVLKDSEISVNMVENILTSSKLTMEERETRYRRLEETWQQADDAWGYLDNAR
                     LTDREGRIWEQLKGPWENWRNAHLEFLSLSRELDETKILHPMELRYELALRQKDHALW
                     IWQLLQSITHGTEFEGQPDPTKCALGQWLNSFITENGELSSYMEEIHEYHNKVHESGG
                     TIAQIILENPGDTDAAMGVYQDITLPNMDRVLGLLSHMDDVAKKADTLFEDMVEHALY
                     VKWDAFIETEAILDQLVAENAKAVEDMRKTADQKAGFALLFLVISILAGIALAGVLAV
                     VLAHRITGAIRDVQDMMAKAAEGDLTVRGRAIGKDELGQLGHSFNRFMVRIQAMTRDI
                     YDTTVTLNTSSQSLLGISENMAANSQEVSAKTGVVSAAVEEISVTIDNAALASEETSH
                     NINTIASAVEEISASTGNLAGAAGQTSANVEDVVRLVEDISLSINNAAASASDVSDAV
                     SSMATSVKEINMSLTEVSVNCERSTEITLDAEARTHETNGMIERLNISSRQIGRIVDV
                     INDIADQTNMLALNAAIEAAGAGEAGKGFAVVANEVKELAKQTAEATDEIGDQIADMQ
                     EQMAGVVAAVGVITEVIDESNKITNTIAAAVTEQSALTGEISLSTTEVAKQVNLISNE
                     IGDIANNSRNAAGSLAEASDGVRGIARSTSELSGATGEIAENTENASVRVGDIAGSSR
                     DIAMGAREINENIQEISTATEDTAIGAGRTSDAAAELAELARRLDTLVRQFKV"
     gene            complement(10654..12993)
                     /locus_tag="VFD89_04450"
     CDS             complement(10654..12993)
                     /locus_tag="VFD89_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZJ57472.1"
                     /translation="MGNSKNHRSKSLKVKIPFLAGILILVILAFVVVTAMRIAGNLLI
                     DNSKASIQSMADTHGEMLDHFMNERIGDVRILANNRDVVDTTASVSLKQDTLSHAITQ
                     HGETYHDMLMVDMSGKLIASSSGNVRNDYSDASWFMNTKEKQDIYYEYRMSRDLHQNI
                     VTFSYPILDDLRKPVGILTARMSDKIIHDMMEDLIEDLLEQGNTGSYPYILDEEGTVV
                     WHPVSDKIGNENIAGRDDFLGETARKMIAGESGSGEYTYDGVEKLVGYAYLPGIGDFK
                     GLGWSLAITLNRDIFMQPIKRFNLMVLLVGGSLSILGLFFLWRITVSSLRPLDETIVM
                     LEAISRGQGDLTIRIPETSRDESGRLARYFNQFVEKIQIMTREIYDMTLALSGSSGTL
                     TDVSNTLAANSEEMNAKTDTVMAALEEITVSIDDTAHASNDTRNNMGIAASAIEEMSA
                     STFNLAGASEETSGNVEQVRIGIQEISDMVTRVAGSAAQVSLSVNGGATAIKELNISL
                     GDVSSNSERSMVIISGAQQRAQQTNEIIRRLNTSSKQIGKIVNVINDIADQTNMLALN
                     AAIEAAGAGEAGKGFAVVANEVKELAKQTAEATEEIAQQIEDMQNNMGEAVGAVETIN
                     QVIEETTQITNAIAAAVTEQSAITGEISTSILEAAKEVNLISDEIQDVAEKSEDAAQN
                     AGKASAGVQEIARSAQELSLAANEVARKTEESSGKMDQIALSTNEVSKGVSDITLSTQ
                     EINQAAHEAAMGASDTNRASVELAELGVKLEQLVRQFKI"
     gene            complement(13054..13479)
                     /locus_tag="VFD89_04455"
     CDS             complement(13054..13479)
                     /locus_tag="VFD89_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZJ57473.1"
                     /translation="MERILTFYLEGELFGIDVGLVKEINRKIKYTQVPGAPGHIVGLY
                     NMRGQVVTIFDIACILHNRESHIPQGPTCIILKNDNSGSEHLGFIVDRPGDVLDVRDE
                     WRQPLPENVEHEYGQYIKEIVRLEDELAAVIDIDRMFGK"
     gene            complement(13479..15680)
                     /locus_tag="VFD89_04460"
     CDS             complement(13479..15680)
                     /locus_tag="VFD89_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HZJ57474.1"
                     /translation="MLKNEEFIQEFIEEAGSHIEQIESHLLELAGDVTDQELINQLFR
                     SVHSIKGTAGFFGLDNIVNLSHSMESLFGAVRSGGVYLDDLSIDALLEANDCLGIMVK
                     DPAKSHEIDISHYTHNLDTILSEDTKKVEGDSVGTSHEPPSPQDHIQPAGGNIPTVLV
                     EDSIRVSIGLLNDLLNLTGELVLGRNQLLRGLEGHRKHVEGLDPVLQNIDSITTQLQE
                     RVMQTRMQPLGNVFNKFPRIIRELSRQLKKEIRLEMEGTQVELDKSIIESLADPLTHL
                     IRNAADHGIEKPGQREDTGKSREGLITIKASQEGGYVSILVADDGAGIDVNFLKNEIL
                     KKGLRTQEELSLMEEKEILGQILLPGFSTAGSVTDVSGRGVGMDVVRTNIERMGGTIE
                     IDSQAGRGTTFKLNLPMTLAIISSLIVESGGQNFALPQVNLQEMIRVKEGDKDRSIEI
                     INGFPVLHFRHKLLPILSLSQVLDLPEDLPQPGKEDNHRTIRILVLKMGERRFGLAVD
                     AIRDDEEILVKPLPRHLKDLKCYSGVTILGDGKIAMILDAEGLASQGELKFPEAGSDP
                     KKKQDIKQAATGDRTMLLFSCSGAETLGLELSLVSRVEEIKHDDIELIGEREYIEFRG
                     RSLELIRPEEHLPISRNEYKDQRLYVIIPKVTGPPVGLLIGKIHDSVSLSWDFNRQDI
                     KAHGIMGTTFWNSRTVLALDLPELLQKAAPEDYGAGDSDTMGSGAGRGGRY"
     gene            16299..17666
                     /locus_tag="VFD89_04465"
     CDS             16299..17666
                     /locus_tag="VFD89_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZJ57475.1"
                     /translation="MGFNGNDIIMKDLPDELRISAEANNFIPIELHKNYNTKRGLRNS
                     DGTGVLVGLTQIGDVLGYYFEDGKKIPMEGRLLYRGIDVGDIVAGFQNDKRFGFEETT
                     YLLLFGRLPDAAQLKEFEEFLGQNRALPDGFAEDMIFKAPSIDIMNKLARSVLASYSY
                     DEDPDNISIRNVLKQCIDLIARFPAFVACAYQAKSHYHNGESLHIHYPQRGLSTAENI
                     LYLVRPDNKFTRLESELLDLSLVLHAEHGGGNNSSFTTHVVTSSGTDTYSAIAAAVGS
                     LKGPKHGGANAKVMAMMEDIKANVHNWSNDREVAHYIRKILRREAFDRSGLVYGMGHA
                     VYTLSDPRAVLLKEKAAQLAKVTGMEQEFDLYRSVERLSPDIFCDEKKDTKTISANVD
                     FYSGFVYKMLNIPEDLYTPIFAMARMPGWAAHRIEELVSGGRIIRPAYKNVLPDHEYI
                     PLKDR"
     gene            17887..19041
                     /locus_tag="VFD89_04470"
     CDS             17887..19041
                     /locus_tag="VFD89_04470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZJ57476.1"
                     /translation="MRKVVLILIVSLSIFSLYLPMSSLGAPPDNSARAAVLMEVETGR
                     VLYEKNPHEKMPMASTTKIMTAILAIENSHPTDIVKVSPRASEVEGSSLYLVTGEKLT
                     MEQLLYGLMLRSGNDAAVAIAEHLAESVDQFAAMMNAKAKEIGALNTNFKNPHGLHND
                     QHYTTAYDLALIAAYAMKNATFREIASTKYYRIPWQDQPWDRVLMNKNALLWEYHGAN
                     GIKTGYTKAARRCLASAALRDGMQLVAVVLNCQPWFDDSGALLDYGFGEYQMIELFSE
                     GEIVGNIPVKNGFKKEVRLSIKDDISAPLTDQEQEDISITLKHPEYLKAPVTADTRVG
                     SIHISLGNNFTISKDIYTTSDIKENSFPSNLRSIIRQWMNNAWDSPIPGP"
     gene            19185..20237
                     /locus_tag="VFD89_04475"
     CDS             19185..20237
                     /locus_tag="VFD89_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HZJ57477.1"
                     /translation="MKNRMFLPITIKGKSIRNRVVMPPMVYFGKAENDGLVTLGHVAH
                     YEARAKGGTGLIIVEATCIDPDGRLSDDQLGIWSDEHIPGLRDIVEACHGYGAVVMLQ
                     IHHAGIKSPPSVTDKPVAPSPVEGKSAVELTLGDIETIKNKFVKAVLRAKKAGFDGIE
                     LHGAHEYLLNQFTSKVTNGRTDEYGGDLKGRMKLSLDIIGDVRKELGNANFIIGYRMG
                     GNDPTLDDGIKIAKLLEDAGVDLIHVSSGIADQEIPLPETPKNFPFNWIVYCGTIINK
                     HVSIPVIVVNSIRTGEQAALIIENRLADFVAVGRGHLTDAEWTNKAMDKLEAISCLEC
                     KPRCHWFTNGDDCPRN"
     gene            complement(20265..20756)
                     /locus_tag="VFD89_04480"
     CDS             complement(20265..20756)
                     /locus_tag="VFD89_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZJ57478.1"
                     /translation="MERETFDVMIEIPKGSRNKYEYDPGKRAIRFDRMLFSPVHYPAD
                     YGFILNTLALDGDPLDALVLVWESTFPGCLIETFPIGLFMMRDEKGEDEKILCVPKND
                     PLWNHIEDLEDVPPHLLREIEHFFAIYKDLEHKEVSIEGWKDKDAALEVIRSTQARYR
                     QNN"
     gene            21069..22586
                     /locus_tag="VFD89_04485"
     CDS             21069..22586
                     /locus_tag="VFD89_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HZJ57479.1"
                     /translation="MTKLGFDNDTYLDKQAAAIIKRVNLFQDKLYLEFGGKLFSDYHA
                     SRVLPGFDPNVKIKLLHKLKDRADIILCVGAGDIERKKIRADFGITYDMNVLKLIDDF
                     REWNLDVAGVVITLFDNQPSAVHFRDKLERQGIRTYTHSFTKGYPTDIDTIVSDEGYG
                     ANPYIETKNKLVIVTAPGPASGKLATCLSQLYHDYTMGLESGYAKFETFPIWNYPLKH
                     PVNIAYEAATADIRDSIMIDPFHLEAYGETATNYNRDVEIFPVIKRILNKITSKSAVY
                     KSPTDMGVNKLSLGITQDDVIQEASKQEIIRRYFWYNVEFIKGFIDQETLTRAELLMK
                     NLGLDQEDRKVVSPARKVVSKRRSGATDDTYESLSAAAIELADGTIVTGKNSYLMDAA
                     SSLILNAVKTIAGIPDNIHLLSPNVIKSIQRLKGDILGEKTKALNLEETLIALGISAT
                     TNPTAQLALGCLKNLAGCEVHFTHIQSPTDEKALRKLKVNMTADPQFPSKSLFMI"
     gene            complement(22711..>23734)
                     /locus_tag="VFD89_04490"
     CDS             complement(22711..>23734)
                     /locus_tag="VFD89_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZJ57480.1"
                     /translation="LKLFIYRIDRRNGMKKILVTQQLTGPGMDTLKERFQVTHPNGNR
                     YTRDEILNIIHEFDGILAAGNRMDAEIIGAARNLKIISVYGAGYDGIDVEEASRRNIL
                     VANAPESVTEPTAELAFGLMLATARNIAAKDRWLRQNPGSKWGMMMREGQSLYGKTLG
                     IVGLGRIGKAVARRGLASGMKVFYFNRTRLGTDQEKDLGADYLPLDQLLQESDVVSLH
                     TPLTPQTHHLIGEDQLNRMKKSAFLINTARGEVVDEKALIRHLEQGNIAGAGLDVFEA
                     EPHIPMGLLELDNVVLTPHIGTATHEVRLEMTVEACQNIIDFFDGKMPASTINPQVFS
                     SFGTEG"
BASE COUNT         5759 a         6224 c         5257 g         6494 t
ORIGIN      
        1 ctaaaatact atgtaataaa gttttaataa ctacgttgtt atgttatact ctattattcc
       61 ccattttcgc caggtcaaac attccacaaa aaatattttt aaataaaaaa agacgatcaa
      121 agaccgcctt ccaatcacga aaattatttt taaaaaatga gcggtcaaag accgccctct
      181 aaatgatcga taaattgttt tgcagtacgt ccggaacggc cgttatacct cttctcccat
      241 cttaaggcct cggcgtgcag agcttccctg ccgatatcta ttccgcgttc ctctgccatg
      301 gactccacca tctcaaggta ccctcgctga tttggggata taaaggtcag ggtaatccca
      361 aatcggtccg ccaaggacaa cttttcttgc atggaatccg taggtgatat ctcgttcctg
      421 tcgttgtatt gatatccggt gctgctcctg tcggcgacta cctcccttac caggtgcctg
      481 cggttggagg ttgcataaac caataggtta gacggcctta ctttaagccc accttccaac
      541 agcgccttga tatgcttata ctctacctca tgctcttcaa aggagaggtc gtcgatgaac
      601 agaataaacc taaacatcct gtcctctata tgccggagta tggagtaata atcgtccaag
      661 tgaatcttgg gcagctcaat tattctgagc cccctgtccc aatacctatg gatcagggct
      721 ttgaccatgg aagattttcc tgtaccctta tccccgtata aaaggacgtt gttggccgag
      781 tatccggata taaacttttc ggtgttatcc aatataattc gtttttggat atcatatccc
      841 acgagccggt ccatggtaat ggggtcggtg tttttaatcc ccttaagggc agtaccgtcc
      901 caatagaacc cgcgatacct gctgaacaga ccggagcctt ttaggctgta ataatctatt
      961 aaggcgttaa cgttccaacc ggcaagaccc ctccgggcgc agacttcttg ccaatttggg
     1021 gtttgtctgc agtggaatat atttaccggg cggaaaccct ttatttcccc ttccagaaga
     1081 tccgcaaggt cctgccacgg gatattcgcc agcttttgga tggaggggag atcattttcc
     1141 gccgcagctg tcatagcccg gggaatatac ccctttcgtg tttctgccca aagggtaaat
     1201 ctattctctg tatgcagcac agcgtctatg acccagtcct tccacgaata ctcccccggg
     1261 gcatgcgtcg ggattgagga caagaaggca tttaaaaagc cggaataacc ctctgccgtc
     1321 tgtgcggggc tccaggagtt ttccgctatg ccctccagca attgcgttaa gcgccttaaa
     1381 gcgggcagct cggaaagttc tttaaagagg atcaggcccc ttgatgccat tataatttcc
     1441 ctaagctcct ccatgaaggc ccctgcgtct attttttgct tattaatcat catgtctacc
     1501 cccatgttcc ttccataagt cattatttga ttgcatctat atggcagccg tttaaatcta
     1561 agtcaaactt aaggtccttg gatttaatag tcctttgaat tcataggcag gacaaaacct
     1621 acgcttcact atttgtttta tgccctgtcc ctgtttgccc tatacatagc cgggcccccg
     1681 cgccttccgg agttaggctg ttatctgctg cccataaccc ttttgtagat gctcctaacc
     1741 ttagaataca ccttgtccga atcaatcgtc aacagcgcct tatttttcat aagcctcttg
     1801 ccgttaaccc atacgctgtc aacatcccct gaatgcccgc tgtacaccag gtgagttgtc
     1861 caatccccgt gggggtggta atggggctct ccggtatcca ccagcactat atcggccttt
     1921 ttcccggggc ttatgctgcc tatttcatcc tctagaccca gagccccggc cccatggttt
     1981 gtagccatct ttaaggcggt agcagcggat ataagggtag catcctcctt cacacccttt
     2041 gatatgaggg cagcgaaggc catctccttc atgatgctca gattgttgtt gctggcagcg
     2101 ccgtcggtac ccaaggaaac cgcaatcccc ttagagagca tctcatggac gggggcgatg
     2161 ccgcttccca gctttaaatt gcttatggga caatggacga ttttaacatc cctatccttc
     2221 aggacatcca aatcctccga ggataggtgg acgcagtggg ctgccaaggt gttgcccccc
     2281 agtagaccca gatcgtttag gtgcatgacg gggctcttgc catattcctt tatacaattc
     2341 tctacctcct gccgggtttc ggacaggtga atgtgtatcc ctgtccccag gctatgggcc
     2401 aattctaaga cctccgcaag atatgacggt tcacaggtgt ataccccatg agggcctacc
     2461 atcacccgta tcctcccatc cccttccccg tgccagttct cccaaaggat tcgattctct
     2521 tccaatctgt cccgagattt ttggtcaggg ccctgaagtc ccctggccaa aaccccgcgt
     2581 atcccggaat ggagtacgga aagggcagtc tgttccataa acatgtacat atcggcaaag
     2641 gcggtcaccc cgccttttat catctccgat atggccagta gggacaacca atagacatcg
     2701 tcctgggcta atctgtcctc tgcaggccaa atcttttcct ccagccattt cataaggggc
     2761 agatcgttgg catatcctct aaagagggtc atagccgtat gggtatggct gttaactagc
     2821 cccggaattg ccagcattcc cctgccgtct tcgactatat ctatatctgt gcccgctgct
     2881 accccaaggt ctctctcgta gcctacaaag gtaatctcat ggccgtcaat gaggatgcaa
     2941 ccgctttgat attgattgga gttttcatcc acggtcagaa tgtttatgtt ttttatcagg
     3001 gttttcatat gatctccttt taatcaatgg attttaataa acttttaatc ttttattacg
     3061 gatatatccc aaaacagcct ttaaatattt accttaatat gatataatta ccacaatggc
     3121 tctcttgcgc atatcataat ctctttcaat actttatact attacacata caacaattct
     3181 tgaacgttat cagacaagag ggagctaaat acgtttaggg aggaattgtt taatgcctac
     3241 accacaatat gttaaggagt ttaacgcaga tctgggagaa ttatcgctta acactggtgc
     3301 cggtaaacat tggaaaagat tcgcagccaa caatattgca gccatacgta agacgacagt
     3361 ggagagaaaa aaattattta ttaccaggac cgttgatagg ttagagattg atatcaacgg
     3421 tgaagaggtc ccatacatca tagaaaaagg cgtcataagg gatgatttcg aatgggtagc
     3481 ggatgtactc aaatcctttg cagagaagaa taaggtagat taccacgagt aatggaaagg
     3541 tccaacggac ttttccatta cttttttctt atgatgatta cggcgacgtc gtccctcagt
     3601 ccctctgggc tatccttacc gaagtccttg actatggaat ctaatagcag tgcaggatct
     3661 tcccttatca tatccttgtg cttctctata taatctaaaa aatcattgca ggttttcata
     3721 tcacgccctg ctatcctagg ctccaacagg ccgtcggtat acatgaatat ataatccccc
     3781 ggacttatcc ttctgctgta tgtcctatat ctatagtctt ccatgacgcc cagaatgagc
     3841 ccgttctcct ccagcatata cgtcctgttc tccttagaat ccaatatcac aggatagggg
     3901 tgccccccgt tacaatatgt aagaacccca tcctctataa ggcccaaaaa agctgttatg
     3961 taataggttc catgggtcag attatagaag gtctcgttca tgctctcgag aacctgggca
     4021 ggggcatgat acctgtcgac caagttttta aacattacct ttaccataga cgagatcatc
     4081 gcagaggcta ccccatggcc cgaaacatcc gctattaaaa gccaggtcct gtcccccgtc
     4141 tgaaggcatt cgtagtagtc cccgctaagc tcggcacttg ggatatatct gcctatcatt
     4201 tctataccgg gcaaggattt ggattcgtct attaaaatgc tctgaagggc gttggcaaat
     4261 tccagctctt ccctcatcct ctgattcatc tggaccaaca accgtttttg ctcataggcc
     4321 tttagggcgt tctttacctt caccggcaat actacaccca tctgctccat cgtcaagggc
     4381 ttggtaaaat aatccgtcgc ccccagttct agcgctttct ggataacacc cacctcatcc
     4441 acggcggaat ttactatgat gggaatatcc ctgtaaacct ccgaggactt gagctccttt
     4501 aagacctgaa acccgtcctt gttgggcatt atgagatcta aaatgattat atctaccctg
     4561 tttttaaata tgtacctaag ggccttttcc ccgtcctccg cctcatgaaa atcggcgtcc
     4621 tctatcctct gctccagggc ccttttaatc agccttctgt taaccaacat gtcatcggca
     4681 atcagaattt tgtaagccac cttacccatc ccctatacta tcccggaaat tgcacttatg
     4741 atcatgtaat cgttaaaggg ttttttcaaa aagtcttcga tccccaaagc ctttgcccta
     4801 tttacgattt gctcgtcccc catagcgctc aacatgataa tccgagcctc ggggtctatc
     4861 cccatgatct cctcggccgc ttcaagcccg tccattacgg gcattgttat atccataaat
     4921 accagatccg gttttaatga ttggtataat gagacccctt cccgcccgtt tttggcatca
     4981 ccgcaaacct cgtatccgtg ggcttccagg gtctttcgaa gaagtctatg aattataggc
     5041 gaatcatcta ccaacaatat cctcttaatc gcactttcgt ttcccactat ggtgttcatc
     5101 tcccttaagt tccaatttaa atatccgcga cttactcaat tctaccataa ccgacctttt
     5161 ttttctataa ttccagacca atatttacct ctaccctacc cagagaggat tccatgtcta
     5221 tctttagggt ctctacggag ctgataataa agtaaacatc cctaccgaag atgatggacg
     5281 gaggtgtgat atccacctgt attccggatc cggacagcag ggtcgacgca tttcccgtaa
     5341 ccatattgga aaactccccc agggcgcttc gccccatctc gttcagttcc tccacaggca
     5401 tgcccatcat catggcggat actatgcgtt tcgccgtgtc cgtcgccatg gagtaggcga
     5461 tatttcccct tacccggcct accatgccca ccacagccgt tatgtccgta tccacgtgca
     5521 tgagatcgtt cttagtcata tttccccttt tgatatccgt agcaccaaac tgttccaata
     5581 tgccctttag ggcctccaaa aatgggagta tgtatttaat gtccatcaac tgctgccccc
     5641 ttcccaggct atattgatcc gtatcatccc gaaatcagtt ttcccatggg atgaaaagga
     5701 ccttaattta gggattgaaa taactacgtc gctgcccgcg aagaccaccg gcggggccag
     5761 cctcaagtcc attaaaaaat cgtcgttcag tccggtgaca gcgtttcccg atattatatt
     5821 gtttacctcc gagatcagag ataataccat gggatccttt atatcatcgt actcctcgcc
     5881 caaaatggtc ccggccaaag aaagggcaag gctcggttcc atatcgataa gcagcctgcc
     5941 ctttttggcc ccggcgaagg tgatgatgga cgatattccc atggaggtca gttcatgggc
     6001 ctcctccttt agagaatcca tccccgagat atctatgtcc gacatctcct tgataatatc
     6061 caaggtgctg atacaaaagg gattgtaaag tctttcatcc atagctaggg caccccctcg
     6121 tcaaagtatt aaacacaggt tgaaatttca taaaaccggc tcctttacct tataatatac
     6181 gcccccgtcg tggctcatcc tgacaaaatt attcccgtac tcgttaaaaa cttcggaatt
     6241 gccgagaaaa agcaccccgc agggcaaatt taatgccccg gcaatctttt taaagatatc
     6301 ccgcttaaga tccggggaaa aatatatggt cacatacctg caaaagatta catcgaactt
     6361 acctagggtt ataaagttat tttgcaggtt gaactgccta aacctcaccc tgtcccgaat
     6421 cctattatgg aggacccaat ccctgctctc tcgggtaaag tattcgttcc tgtagtctaa
     6481 atccagaccc ctcatgatgg acacgtcatc gtatcttccc gcctccgctg ccttcagcac
     6541 gctgcgggat atatccgtag ccaatatctc aaagtccttt ggggacaccc cctggatcct
     6601 tcgctgtgtc agatatctgt ctatgctcat ggctatggaa tagggctcct ggcccgtgga
     6661 gcatccggcg ctccatatcc tgaccctgtc ccgtttttta ttctggattt cgtctatgta
     6721 tcttggtagc agcacctcct ccaaggtctt aaagggagtc ctgtcccgaa accacagggt
     6781 ctcgttggtg gttatggcat ctatcacctt ctcctttatc ctcgggtcct tttcactctt
     6841 tagggtaaag tagagcccct cgaaggagtg tatattaaat tccgtcaata tcctcgaaag
     6901 ccggctcgtg ataaggtagg ccttatcctt tcccaataca attccgcatt ccttttctat
     6961 gtagccacgc ataagttcga attcccttgc agttaagtcc cgcatcagca tctaccccta
     7021 tttatctcta tgatccttct accgatatcc tttaaatcca ctacctcatc ggagagcccc
     7081 gcctcaaaga cgcttctggg catcccgtat atgacacagg tttccctgct ctgggtgata
     7141 caataacact ggcaggcctc cttcagggcc ttgacgcccc tggtgccgtc acttcccatt
     7201 cccgttaaga tgacggccaa cacccttttg cccgcatagg cctcggccac ggattcaaac
     7261 aggacatccg cagcgggcct taccccattt acaggcggcg acgtagtgag ggtgatctga
     7321 ggcccccggg cattaactga catgtggtat cccccgggag ctaccaacac ctgcccgccg
     7381 acaattttgt ccccctcttc cgcttccttt acgggcattt cacactttct gcctaagaac
     7441 ctggaaaata tcttggtaaa gtccggcggc atatgctgga ctatcaacac gggctttgta
     7501 aacccgggcc caatcccctg gcaaacctct tccaccgcgt tgggaccccc ggtagaagag
     7561 gcgataacca ccaggtctat tccgctgagg catggtgtgc catagccgga tgctctgtcg
     7621 gctctggggg ggcgtttccc gtccccgggg ataatgccgg agcctcttgt aaggctttct
     7681 ccctccctag cccgggaagc atcgagcctc cttccggctt ttgggatggc gtccttactt
     7741 acttgattct cccgattatt tatggcgtac ctgtctatca agatctggga aaacaatatg
     7801 ttaagatgtc tgctgatggc atcaatgttc ttgttcccat gcccctggga gggcttttgt
     7861 ataaaatcca tggccccata ttccaatgcc ctcaccgtta attttgcccc gtctaccccg
     7921 ccgctgctga tgattacaac gggcaaatcc ccatacttgt cctttatcct tgtaagggtc
     7981 tctaaaccgt ccatcccggg cataaagaca tccatgagca caacgtctat gggtctttgt
     8041 tccagccgct ctagggcggt tatcccgctg gatgccgtac agtccacaac agccttatcc
     8101 gtcctctcca ccgccctaga gattattttc ctgtatacca gcgaatcgtc cactaccaaa
     8161 accttaagtt tgtccataaa atgtctcccc cttagaattg atcaaacctt gaattggcga
     8221 accaacgtat ccagtctccg agccagttca gccagttcag ccgcagcatc gctggtcctt
     8281 cccgctccta tggccgtatc ctcagttgcg gtgctaatct cttgaatgtt ctcgttgatc
     8341 tcccgggcgc ccatggcgat atcccttgac gatccagcaa tatcccccac cctcacggat
     8401 gcgttctcgg tattttcggc tatctctccc gtggccccgg acaattcgga ggtggatctg
     8461 gctatcccgc ggactccatc agatgcctct gccaggctcc cggcggcgtt ccgtgaattg
     8521 ttcgctatat ccccaatttc gttactgatt agatttacct gtttggctac ctccgtggtg
     8581 gacagggata tctcgccggt caaggccgat tgttccgtta ccgctgcggc tatggtattg
     8641 gttatcttgt tggattcgtc tataacctcg gttatcacgc ccaccgcggc tacaacccct
     8701 gccatctgct cctgcatatc tgcgatctga tcccctattt catccgtggc ctcggctgtt
     8761 tgctttgcca attccttcac ctcattggca acaacggcaa agcccttgcc cgcttcacct
     8821 gctcccgccg cctctatggc agcgtttaag gccagcatat tggtctgatc cgctatgtcg
     8881 ttgatgacat caacgatcct tccgatctgt cgggatgaga tattcaatct ctcgatcatc
     8941 ccgttggttt catgggtacg ggcctcggca tccagagtta tctctgtaga acgttcgcaa
     9001 ttcacgctga cctccgtaag ggacatgtta atttccttga cagaggtggc catactgctg
     9061 acggcatccg atacgtccga tgcggaagca gccgcattgt ttatgctcaa ggagatatcc
     9121 tccaccagtc ttactacatc ctctacgtta gccgatgtct gccccgccgc accggcgagg
     9181 tttccggtgg aggcggatat ctcctctacc gcagatgcta tggtgttgat gttatggctg
     9241 gtttcctcgg atgccagggc agcgttgtct atggttacgg atatctcctc cacggctgcg
     9301 gagaccaccc cggttttggc gctgacttcc tggctgttgg ccgccatatt ctctgatatc
     9361 cccaataggc tctgggacga ggtgttcaag gtcacggtgg tgtcatagat atcccgggtc
     9421 attgcctgga tcctgaccat aaaccgattg aaggaatggc ccagctgacc cagctcatcc
     9481 ttgcctattg ccctgcccct taccgtcaga tccccctccg cggccttggc catcatatcc
     9541 tgtacgtccc gaatcgcccc ggtaatccta tgggcaagga ccacagccaa taccccggcc
     9601 agggcaatcc ccgccaaaat ggaaatcacc agaaacagaa gggcaaatcc ggccttttga
     9661 tcggcggtct ttctcatgtc ctctacggct ttggcattct ctgcgaccag ttgatccaaa
     9721 atggcttccg tttcgataaa ggcatcccat tttacataca gggcgtgctc caccatgtct
     9781 tcaaataggg tatccgcctt ttttgccacg tcatccatat gggacaagag ccccaagacc
     9841 ctgtccatgt tgggtagggt tatgtcttga taaaccccca tggcggcgtc tgtatccccg
     9901 ggattctcta ggataatctg cgctatggtg ccgccgcttt cgtggacctt gttatggtat
     9961 tcatggattt cctccatata ggaggaaagc tccccattct cagtgataaa tgaattaagc
    10021 cattgtccca gtgcgcattt cgtgggatcc ggctgcccct caaattccgt accatgggta
    10081 atggattgga gaagctgcca tatccacagg gcatgatctt tttggcgcag cgccagctca
    10141 tacctgagtt ccatgggatg gagtattttt gtttcgtcca attccctgga cagggacaaa
    10201 aattccagat gggcgttcct ccaattctcc caaggaccct tcagttgctc ccatatccgc
    10261 ccctccctat cggtgagccg ggcgttatct agatatcccc aggcatcgtc ggcctgctgc
    10321 caggtttcct caaggcgtct gtatctggtc tccctctcct ccatggtgag ctttgaactg
    10381 gtcaaaatgt tttccaccat atttacgctg atctcggaat ccttcaggac caatatgttt
    10441 ttcaccttgg ggagctctgc ctcccccata tcatcgagtt ccttcattac tcccgtcatt
    10501 ccccaatagc ctaccaatcc aattaccagc gcaaaggcgc atatcgcgga aaacgacaga
    10561 agtagcttgg atcttatcct catgaaaccc ctcctgttta cggataaaaa tacccataaa
    10621 taattttctg tttaaatgta ccgcatgata cggttaaatt ttaaactgcc ttaccagctg
    10681 ttccaactta acacccagct ctgccagctc cacggaagcc ctgttggtat cgcttgcgcc
    10741 catagccgct tcgtgggctg cctgatttat ctcctgggtg ctcagggtta tatcgctgac
    10801 tccctttgat acctcgttgg tggacagagc gatctgatcc atctttccgg aggattcctc
    10861 ggttttgcgt gccacctcgt tggccgccag ggacagctcc tgggcggatc tggctatttc
    10921 ctgtacaccg gccgaggcct ttcctgcatt ctgggccgca tcctcggatt tttcggccac
    10981 atcctgtatc tcgtcgctga tgaggttgac ctctttggcc gcctctaaaa tagaggtgga
    11041 gatctccccg gtaatggccg attgttccgt taccgccgct gcaatggcgt tggttatctg
    11101 tgtcgtctcc tctatgacct ggtttatggt ctctacggcg cctacggcct cgcccatatt
    11161 attttgcata tcctctatct gctgggcaat ctcctctgtg gcctctgcag tctgtttggc
    11221 caattccttt acctcgttgg ccaccacggc aaaacccttg cccgcttctc ccgcacccgc
    11281 tgcctctatg gcggcgttta gggccagcat attggtctga tccgcgatat cgtttataac
    11341 gttgactatc tttcctatct gcttagagga cgtatttagc ctccttataa tctcgttggt
    11401 ctgttgggcc ctctgctggg ctccggatat tataaccatg gaccgctccg aattgctgct
    11461 tacatcccct aaggaaatat taagctcctt gatggccgta gctcccccat ttactgacag
    11521 ggatacctgg gctgcggaac ccgctaccct ggttaccata tcggaaatct cctgtatacc
    11581 tatcctcacc tgctccacgt ttccggaggt ctcctcggaa gcccccgcca ggttaaaggt
    11641 agaggcagac atctcctcta tagctgaagc cgctatgccc atattgtttc tggtatcgtt
    11701 ggaggcatgg gcggtatcgt ctatgctcac ggtgatctcc tccaaagccg ccatgacggt
    11761 atcggtcttt gcgttcatct cttcgctgtt ggcagctaag gtgttggaca catccgtaag
    11821 ggtaccggag gatcccgaca gggccagggt catatcatat atctcccggg tcattatctg
    11881 tatcttttca acgaattgat taaaatacct ggccaatctt ccggattcgt cccgggaggt
    11941 ctcgggaatc cttatggtca gatccccctg tcctctggat atggcctcta gcatcactat
    12001 ggtttcgtcc aaaggcctta ggctagatac ggttatccgc cataggaaga atagccccaa
    12061 tatggacagg cttcctccca ccaacaaaac cattaagtta aacctcttta tgggctgcat
    12121 aaagatatcc ctgttaaggg tgatggccag ggaccacccg agacccttaa aatccccgat
    12181 tccaggcagg taagcatatc ccaccagctt ttctacaccg tcataggtgt actcaccgct
    12241 tccgctctca ccagcaatca tctttcgagc cgtctcccct aaaaaatcgt cccttcccgc
    12301 aatgttctca tttcctatct tatccgaaac cgggtgccaa accacggtcc cctcttcgtc
    12361 caatatgtat gggtagctgc cggtattgcc ctgctccaac agatcttcta tgagatcctc
    12421 catcatatcg tgaattatct tatcggacat acgggccgtc aatataccta cgggttttcg
    12481 taagtcatcg agaataggat agctgaaggt gacgatattc tggtggagat ccctgctcat
    12541 cctatattca taataaatat cctgcttttc tttggtgttc ataaaccagg atgcgtcgct
    12601 ataatcgttt cgcacattgc cgctgctgga ggctatcagc tttccactca tatccaccat
    12661 tagcatatca tggtatgtct ctccatgctg ggttatggca tgggacaggg tatcctgttt
    12721 taagctgacg ctggccgtag tgtccactac atccctgttg ttggccagga tgcgcacatc
    12781 cccgatacgt tcattcataa agtggtccag catctcgccg tgggtatcgg ccatggactg
    12841 tatacttgct ttggaattgt ctatgaggag gtttccagct atcctcatgg cggttacgac
    12901 tacaaaagcc agtattacga gtatcaaaat ccctgcaagg aaggggatct tgaccttcaa
    12961 gcttttagat ctgtgattct tcgagtttcc catattcggt cttcctttct atctgagcat
    13021 cctatttgct gaacatgcca tttgtcaaac ttcttatttg ccaaacatcc tatctatatc
    13081 tatgacggcg gctaactcgt cctccaaccg tacaatctct tttatgtatt gtccatattc
    13141 atgctctacg ttctccggca ggggctgacg ccattcgtcc cgtacgtcca aaacatcccc
    13201 tggcctgtcc actataaacc ctaaatgctc gctcccggaa ttatcgtttt ttaaaattat
    13261 gcaggtaggg ccttggggaa tatgggattc tctgttatga aggatacagg ctatgtcaaa
    13321 tatggtaacg acctgccccc tcatattgta cagtcccact atatgccccg gggccccggg
    13381 tacctgtgta tatttaatct tcctgttaat ctccttaacc agcccaacgt ctatcccaaa
    13441 cagctcgcct tctaagtaga aggtcaatat tctttccatt aatatcgtcc ccccctcccg
    13501 gcgccggatc ccatggtatc gctgtcccct gccccgtaat cttcgggggc ggccttttgc
    13561 agcagttccg gaagatccaa ggccaagacg gtcctgctat tccagaatgt ggtgcccatt
    13621 attccatggg ccttgatatc ctggcgatta aagtcccagg acagagaaac gctgtcatgg
    13681 atttttccaa tcaacagtcc tacgggcggt cccgtcacct tgggtattat aacatagagc
    13741 ctttgatcct tatattcatt tctggagatg gggaggtgtt cttcgggcct tataagctct
    13801 agggagcgtc ccctaaattc gatatactcc ctctctccga tgagctcgat atcatcgtgc
    13861 ttgatctcct caactcggga caccaaagat aattcaagcc ccaaagtttc ggctccggaa
    13921 cagctaaaca acagcatggt cctgtccccg gtcgccgcct gtttgatatc ctgctttttc
    13981 ttcggatcgc ttccggcctc gggaaatttg agctcgccct gggaagccag gccttctgcg
    14041 tctaaaatca tggcaatctt tccgtcgccc aatatagtta ccccggaata gcacttaaga
    14101 tcctttaaat gcctgggaag aggctttaca agaatctcct cgtcgtcacg tatggcgtcc
    14161 accgccaggc cgaagcgcct ttcccccatt ttgaggacca agatccttat ggtcctatga
    14221 ttatcttctt tccccggttg gggtaaatct tcgggcaggt ccaatacctg ggataaggat
    14281 aggatgggga gcagcttatg gcgaaagtgc agaaccggaa aaccgtttat aatctcaatg
    14341 gacctatcct tatccccctc ctttacccgg atcatctcct ggagattgac ctggggcagg
    14401 gcaaaattct ggcccccgga ttcgacgatg agggaggata ttatggccag ggtcatgggc
    14461 aggttcagct tgaaggtggt tcccctgccg gcctggctgt ctatctcgat ggttcccccc
    14521 atcctttcaa tattggtacg cacgacatcc ataccgacac ctctgcctga cacgtcggta
    14581 acgctgccgg cggtggaaaa accggggagc aatatctgtc ccagaatctc cttttcctcc
    14641 atgagggata actcttcctg ggttctgagc ccctttttaa gtatttcgtt ttttaaaaaa
    14701 ttaacatcaa tcccggcccc gtcatcggcc acaaggatgg aaacatagcc cccctcctgg
    14761 gaggctttaa tcgtaatgag cccttccctg ctctttccgg tatcctcccg ctgcccgggc
    14821 ttttcgatac cgtgatctgc cgcatttcgg ataaggtggg ttaaggggtc tgccaaagac
    14881 tcgatgatgg acttatccag ctctacctgt gtaccttcca tctccagcct tatctccttt
    14941 tttaactgcc tggacaattc ccttataatc ctggggaatt tgttgaacac attgccgaga
    15001 ggctgcatcc tagtctgcat gaccctctcc tgaagctggg tcgttatgct gtctatattt
    15061 tggagtacgg gatccaggcc ctccacatgc tttcggtgac cctccaggcc ccttagcagc
    15121 tggtttctgc ccagcaccaa ttcgccggtt aggttcaaaa gatcgtttag gagcccaatg
    15181 gataccctta tgctgtcctc aacgagaaca gtgggaatat ttcctccagc aggctgaata
    15241 tgatcctgcg gcgaaggggg ctcatgggac gttccaaccg agtccccctc aaccttttta
    15301 gtatcttccg ataggatagt atccaggtta tgggtgtaat gcgagatatc aatttcatgg
    15361 ctctttgcgg ggtctttcac cattataccc aaacagtcgt tagcctctaa cagggcgtct
    15421 atggagaggt cgtccagata aacccctcca ctgcgaacag ccccaaagag gctctccata
    15481 gaatgggata gatttactat attgtccaag ccaaaaaaac ctgccgttcc ctttatgcta
    15541 tgtacacttc taaagagttg atttattagc tcctgatccg taacatcacc ggccaattct
    15601 aagaggtgtg actcgatctg ctctatatgc gatccggcct cctctataaa ctcttggata
    15661 aactcttcat tcttcagcaa acaaattccc tccgttatta tagacagaca actccgacta
    15721 tgaaattttt cattgtaaaa ttgcttagac ccgttgcaat tgtcacgccg gtaaattaag
    15781 gttccgttat gggtcagcgg gcataaatat aggtcaatgg gcacaaagcg ttaaattagc
    15841 ctgttgtgct ggttaatata taggtaatga ccattgaacg ctacctccga tattgattgg
    15901 gccaaattat ggcgtggggt taatataatg catctttttg aaacttctac acagattctg
    15961 tcgtatagct ggttaataac cctattttgg aagagttaaa caattatata acatgcaggg
    16021 gcgatctgtc tttttatttc ccgcaggaag gggaataatc atataggggg ccggaatggg
    16081 tttatttcaa aaccatatag gaagagcccg ggcccgtggc ggttttggtg gattaatagc
    16141 cggaataatg cttttaatca gatatgcaag ttttctgaca tttgcaactt ttaatttata
    16201 aaatgttatt gttttaactt aggatatgtg atatattata tggaatatat ccatatacat
    16261 tgcagtggta gtatgtaaac gagaggtgtt atatgatcat gggatttaac ggaaatgata
    16321 taataatgaa agatttaccc gatgagttgc ggatttcggc ggaggccaac aattttatac
    16381 ccattgagct ccataagaat tacaatacaa agagaggact tagaaacagc gacggcacgg
    16441 gcgtattagt cggcttaacc caaattgggg atgtgctggg ctattatttt gaggacggca
    16501 aaaagatacc catggaaggt cgcctcctct accgaggtat cgatgtcggt gatattgtcg
    16561 caggctttca aaacgataag aggttcggct ttgaggagac cacctatctg ctcctattcg
    16621 gcaggttacc ggatgccgca caattaaagg aatttgaaga attcttaggt caaaacaggg
    16681 cattgcccga cggctttgca gaggacatga tttttaaagc tcccagcata gatatcatga
    16741 ataagctggc cagaagcgtc ctggcttcct attcctatga tgaggacccg gacaatatca
    16801 gtatacgcaa cgtgctaaaa caatgcatag atttgatagc caggttcccc gccttcgtgg
    16861 catgcgccta tcaggccaag agccattacc ataacggcga gagtcttcat atacattatc
    16921 cccaaagggg tctcagtacc gcagagaaca tactgtatct ggtaaggccc gacaacaaat
    16981 tcacaaggct ggaatcggaa ttgctggatc tgtccctggt ccttcacgca gaacacggcg
    17041 gcggtaacaa ctccagtttc actacccatg tggtcacctc gtcgggtacg gacacctatt
    17101 ctgccatcgc agcggctgta ggctctttaa agggtccgaa gcatggcgga gccaacgcca
    17161 aggttatggc gatgatggag gacatcaagg ccaatgtgca taattggtcc aacgataggg
    17221 aggtggccca ctatattcga aagatcctgc gcagggaagc ctttgacaga agcggtctgg
    17281 tctacgggat gggccatgca gtctacaccc tttccgaccc aagggcggtc ctcttaaagg
    17341 aaaaggcggc gcaattggct aaggtcacgg gcatggaaca ggagtttgat ttgtacagga
    17401 gtgtagagag actgtccccc gatatcttct gtgacgaaaa gaaggatacc aagaccatat
    17461 cggccaatgt agatttttat tcaggcttcg tctacaagat gctgaatata ccggaggatc
    17521 tttatacccc catctttgcc atggccagaa tgccgggttg ggctgctcat aggatagaag
    17581 agctggtcag cgggggaagg attatcaggc ccgcatacaa gaacgtcttg cccgatcatg
    17641 aatatattcc tctaaaggat agataaacta tatattacgg ctgttaaacg ccatcacccc
    17701 cataagttaa agggatcgat ccgcagactt ggatcggtcc ctttcttttc tgaatgattt
    17761 ttcaccgtaa acaaataata cccttatgtc ctatgggggt tttcattgga ttggattccg
    17821 ccctggggta ggtgttctgt acctaacatc gcaaaagaat ttcgtggaaa atcgggagga
    17881 aaattcatga gaaaagttgt tttaatactc atcgtatcgc taagcatatt ttcgctctac
    17941 ttgcctatgt cgtctttagg cgcaccgccg gataattcgg caagggccgc agttttaatg
    18001 gaggtggaaa cgggcagggt attgtatgaa aaaaatcccc atgagaagat gcccatggcc
    18061 agtacaacca agataatgac ggccatattg gccattgaaa attctcaccc caccgatatt
    18121 gtaaaggtct ctccccgggc ttcggaggta gagggctcct cgttatattt ggttaccggt
    18181 gaaaaactga ccatggaaca gctcctttat ggtctaatgc tgcgatccgg taatgacgct
    18241 gcagttgcca tcgcagagca tctggccgaa tccgtggacc agttcgcagc gatgatgaat
    18301 gccaaggcca aagagattgg ggcattaaat actaatttca aaaatcccca cggcctccat
    18361 aacgaccaac actatactac cgcctacgat ttggccttaa ttgcggcata tgccatgaaa
    18421 aacgccacgt ttcgagagat tgcgtctaca aaatactata ggattccctg gcaggatcaa
    18481 ccctgggaca gagttcttat gaacaaaaat gcccttcttt gggaatatca tggggcaaac
    18541 ggcatcaaaa ccggatatac caaggccgcc agaaggtgtc ttgcctcggc agccctcaga
    18601 gacggtatgc aattggtggc tgtggtattg aattgtcagc cctggtttga cgacagcggg
    18661 gctcttctgg actatggttt tggggaatat cagatgatcg aattgttttc ggaaggagaa
    18721 atcgtgggga atatccccgt gaaaaacggc tttaaaaagg aagtgaggct cagcataaag
    18781 gacgatatat ccgcccccct aacggatcag gagcaagagg atatatccat aaccctcaag
    18841 catccggaat accttaaggc acccgttact gcggacacca gggtaggtag tatccatata
    18901 tccttgggaa ataatttcac catctccaag gacatatata cgacttccga catcaaggag
    18961 aacagctttc cctccaacct ccgtagcatc ataaggcaat ggatgaacaa tgcctgggat
    19021 agtcccatcc ccgggccctg aggcccgttt tactaatcgg aactcgccac ttatagaggc
    19081 gggagacatc ctgccgactc gttataaaaa aatcaaatgt tgatatataa gtatatacat
    19141 tatacaatat acattaggag gcaaagaatt ggaggcggta aactttgaaa aatcgtatgt
    19201 tcttaccaat taccataaag ggtaaaagta tccgaaatag ggtagttatg ccccctatgg
    19261 tctattttgg taaggccgaa aacgacgggc tggtaaccct ggggcatgtg gctcactatg
    19321 aggccagagc taaaggcggt acagggctga taatagtaga agccacttgc atagatccgg
    19381 atggcaggtt atccgatgat cagctgggga tttggtccga cgaacatatt ccagggcttc
    19441 gggatatagt tgaagcctgc catgggtatg gagccgtggt tatgctccag atccatcacg
    19501 cgggaatcaa gagtccccca tcggtaacgg ataaacccgt agcaccatcc ccggtggaag
    19561 gaaaatcggc ggttgaattg acccttgggg atatagagac aataaaaaat aaatttgtta
    19621 aagccgtgct tagggccaaa aaggcaggat tcgacggaat agagctccat ggtgcccatg
    19681 aatacttact taaccagttc acatcaaagg tgaccaatgg aagaaccgat gagtatggcg
    19741 gagatcttaa gggtcgcatg aaattgtccc tggacataat cggcgatgtt cgaaaggaat
    19801 tgggcaatgc taatttcatt ataggttatc gaatgggggg taacgatcct accctggacg
    19861 acgggattaa gatcgccaaa ttattggagg atgcaggggt ggacctcatc cacgtttcct
    19921 cggggattgc cgatcaggaa attcccctcc ctgaaacccc gaaaaacttt ccctttaact
    19981 ggatagtgta ttgcgggacg ataatcaata agcatgtgag catacccgta attgttgtaa
    20041 atagtatcag aaccggtgag caggctgcgc ttatcataga gaacaggttg gcggattttg
    20101 tagctgtagg ccgtggccat ttgacggatg ccgagtggac taacaaggct atggacaagt
    20161 tggaagccat atcctgcctt gaatgcaagc cccgttgcca ttggtttact aacggggacg
    20221 attgcccccg aaattaaacc ggtacccttt gcggtccggt ttttttaatt attttgccta
    20281 tacctggcct gggtactcct gatgacctcc agggctgcat ccttatcctt ccagccctcg
    20341 atgcttactt ccttgtgttc caggtccttg tatatggcga agaagtgctc aatttccctc
    20401 agaagatggg gcggcacatc ctctaggtcc tcgatatgat tccatagcgg atcgttcttg
    20461 gggacgcata ggatcttttc gtcctcgccc ttttcatccc tcatcataaa caaccctata
    20521 ggaaaggtct caataaggca tccgggaaac gtggattccc acaccaacac aagagcatcc
    20581 aagggatcgc cgtctagggc tagggtattt agaataaatc catagtccgc gggataatgt
    20641 acgggagaga acaacattcg atcaaatcgt atcgctcttt ttcccggatc atattcgtat
    20701 ttatttctgc ttcccttggg tatctcaatc atgacgtcaa aggtttctct ttccatttta
    20761 tcagtcctca tttattattt gcttttatta ttagctagtc atataattag tatgatatca
    20821 aataaaacgt catacagcaa cgtggctata tctatgggct gcataattca aatttggaga
    20881 ataatattcc aatctgcaca ggatatccgt catctatgga catccctttg cgaaggtggg
    20941 tgtgccccgt ttattatgac cattcatgta gaataaaaat ttatattgta tatagaccct
    21001 gccgtctggt attataatac ggtagatttt ttgcaaatac agagattcga ccggggggaa
    21061 caattgttat gaccaaactg ggatttgata atgatacgta tttagataaa caagcggcag
    21121 cgataataaa gagggttaac ctgtttcagg ataaactcta tctggaattt ggcggcaagc
    21181 ttttttccga ctaccatgca tccagggtgc tgccgggatt tgatcccaat gtaaagataa
    21241 agctccttca caagctgaag gacagggccg atataattct atgcgtcggc gccggggata
    21301 tagagcgcaa gaagatacgt gcggattttg gtataaccta cgacatgaat gttttaaagc
    21361 tgatagatga ttttagggaa tggaatttag atgttgcggg ggtagttatc accctcttcg
    21421 acaaccagcc ctcggctgtt catttcaggg acaaattgga gcgccagggt atcaggacct
    21481 atacccacag ctttacaaag ggctacccga ctgacattga caccatagtc agcgatgagg
    21541 gctatggtgc taatccatat atcgaaacca agaataagct ggttatagtc acggcgccgg
    21601 ggcctgccag cggtaagctg gccacctgtc tttcccagct gtatcatgat tacaccatgg
    21661 gcttggaatc cggatatgca aaatttgaaa cctttcctat atggaactat ccgctgaagc
    21721 atccggtaaa catcgcctat gaagctgcca cggcggatat tcgcgattcc atcatgatag
    21781 atccgtttca tctggaagct tatggggaaa cggctacaaa ctacaacagg gatgtggaaa
    21841 ttttcccggt gataaagcgg attttaaata agataacatc gaaatccgct gtatacaagt
    21901 cacctacgga tatgggggtt aacaagctga gcctcggtat aacacaagac gatgtaatac
    21961 aagaggcttc caagcaggag ataatcagac gttatttctg gtacaacgta gagtttatta
    22021 agggtttcat cgaccaagag accttgaccc gtgcagagtt actcatgaaa aatttaggtc
    22081 ttgaccagga agatcggaag gtggtatctc ccgcgagaaa ggttgtatcg aaacggcgat
    22141 ccggtgctac agacgataca tatgaaagcc taagcgccgc cgccatagag cttgctgacg
    22201 gaaccatagt cacgggcaaa aattcctacc ttatggacgc ggcatccagc ttaattttaa
    22261 atgccgtcaa gaccatagca gggataccgg acaatatcca tctattgtca cctaatgtca
    22321 tcaagtccat acagcgattg aagggtgata tcttagggga aaagaccaag gccctaaacc
    22381 tggaagagac ccttatagcc cttgggataa gtgccaccac caacccaaca gctcaattag
    22441 ccctcggttg tcttaaaaat ctggcgggct gtgaggtcca ttttacccat atccaatccc
    22501 cgacagatga aaaggccctt cgaaagctga aggtgaacat gacggccgac ccgcagttcc
    22561 cgtcgaagag cctgtttatg atttaactag ggcagctttc cggtattatt tgaaaggtgc
    22621 ctagaataaa ctattttcct gtaaaacaca attatcgttt aaaatatagt atttgcgaca
    22681 agatcacggt ttcatatagg tgtgaacacc tcacccttcg gtgccgaagg aactgaacac
    22741 ttggggattg atggtagaag ccggcatctt tccgtcgaag aagtctataa tattttggca
    22801 ggcctccacg gtcatctcca acctcacctc atgggtggcg gttccgatgt ggggagtcag
    22861 taccacatta tccagctcca acagccccat tgggatatgg ggttcagcct caaaaacatc
    22921 cagccctgca ccggctatat tgccctgttc caaatgtcgg atcagtgctt tttcgtccac
    22981 tacctctcct ctggcggtat ttatgagaaa agcggatttt ttcattctat tcagctggtc
    23041 ctcccctata aggtgatggg tttgcggcgt caggggagta tgtagggaca caacatccga
    23101 ttcctgtaat aattgatcaa ggggcagata gtccgcgccc aaatcctttt cctgatccgt
    23161 acccagtctg gttctgttaa aatagaatac cttcatcccg gatgcaagac cccttctggc
    23221 aacagccttt ccgatgcggc ccagccctac aattcccagt gtcttaccat aaaggctctg
    23281 gccctctctc atcatcatac cccattttga tccggggttc tgtctcagcc atctgtcctt
    23341 ggcggcaatg tttctggctg tagccagcat aagcccaaag gccaactcgg cagtgggttc
    23401 agtaacggat tccggtgcat tggccactag gatattgcgt ctgctggcct cttccacatc
    23461 gatgccgtcg tagcccgcac catatacgct gatgatcttt aaattcctcg cagccccgat
    23521 tatctcagca tccatcctat tgcctgcggc cagtattccg tcaaattcgt ggataatatt
    23581 taaaatctcg tccctggtat acctattccc attgggatgg gttacctgaa agcgttcttt
    23641 gagggtatcc attccaggcc ccgttagctg ttgagttacc aatatttttt tcatgccgtt
    23701 cctcctgtca attctgtaga taaacaactt caac
//
LOCUS       DASRNX010000113         9735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_22324,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000113 DASRNX010000000
VERSION     DASRNX010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9735
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22324"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            497..1750
                     /locus_tag="VFD89_04495"
     CDS             497..1750
                     /locus_tag="VFD89_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZJ57481.1"
                     /translation="MKTRAVRLYGVNDLRLEEFELPAIKEDEILAKVISDSVCMSSYK
                     AAIQGANHKRVPDDVAENPVMIGHELCGIILEVGKKWQDQFTSGQKFTIQPALNYKGS
                     LDAPGYSYPYIGGAATHIIIPSEVMETGSLLEYKGDAYFYGSLAEPMSCIVGAFHASY
                     HTTPGKYHHDMGIVEGGNMAILAGVGPMGLGAIDYAINCDRRPGLLVVTDIDEARLKR
                     AAEVITVEEAAERGVRLHYVNTAGLNNPVDHLMSFTDGKGYDDVFVYAPVRAVIEQGD
                     GILGRDGCLNFFAGPTNPDFKALFNFYNVHYASTHIVGTSGGNTDDLSESLDMMAKGL
                     IDPSTMITHIGGLNSVVETTLNLPNIPGGKKLIYTHIDMDLTAIEDFREKGKTDQRFE
                     DLAKIVEGNNGLWCLDAEEYLLNNL"
     gene            complement(1887..2651)
                     /gene="srlD"
                     /locus_tag="VFD89_04500"
     CDS             complement(1887..2651)
                     /gene="srlD"
                     /locus_tag="VFD89_04500"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HZJ57482.1"
                     /translation="MEGQIAIVTGGGQGLGQALCQRLHNEGANVVIADINSDAAWDTA
                     NQLTGSMAVKVDVSDYSQCENMVNETMAKYGRIDLLVCNAAILIAKGIDEFTSEEWKK
                     VMEVNLLGFFNCAKAVSGVMLSQGYGNIIQINSKSGKKGSFKNSAYAASKFGGIGFTQ
                     SLALELAPKNIRVNAICPGNLLDSPLWVDSLYEQYARNQGITKGEVRQKYLNQVPLGR
                     GCTYEDVANVLVFLASDQAGYMTGQAINVTGGQEMG"
     gene            3028..4290
                     /locus_tag="VFD89_04505"
     CDS             3028..4290
                     /locus_tag="VFD89_04505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZJ57483.1"
                     /translation="MAIPVIMPRQGLSVESCIITKWHKEKGDAVEAGEPLFTYETDKA
                     TFEEESQHSGVLLDIFFEEGDDVPVLTNICVIGQAGEDPSQYSPHGEAADSLASKEHT
                     EGEPVPIPETGPEDTESSTDVPPIIDQNDDIIRVSPRAKNLAHKAGIDITKAEPTGPE
                     GRIIERDILALMDKKLPLAPPVTAELDTEPGYVEEGLTNIRKVIARTMHESLMTSAQL
                     TLHSSFDATEILAFRSDLKKHGERLGLGRITLNDMILYGVSRTLLNHKHLNAHFLGDS
                     IRIFKTVNMGVAIDTPRGLMVPTVFGADSKSLNQISQEVKQLNEACQAGTINPDILTG
                     GSFTVTNLGTLGIESFTPVLNTPQTGILGVNNIVQRARESHGQIEYYPAMGLSLTFDH
                     RAVDGAPAARFLKELVENLESFRLLLAK"
     gene            4331..6802
                     /locus_tag="VFD89_04510"
     CDS             4331..6802
                     /locus_tag="VFD89_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZJ57484.1"
                     /translation="MPKSQFIDPNEVRKSGFIELDDIPINQYKRTITQEKDNYSKEDF
                     MRIFRDMAIIREFETMINEIKTMGQYNGIEYNHPGPAHLSIGQEASAVGQAYLLDQQD
                     FIFGSHRSHGEILAKGLSAIYGMGDEDLLSVMEEFFDGSILRIVEGRQKNAGSVKELA
                     IDFLLYGTLAEIFARETGFNKGLGGSMHAFFTPFGIYPNNAIVGGSADIALGSALYKK
                     INQKGGIVIANIGDASMGCGPVWEAMNMAAMDQFKLLWDESHRGGLPIIFNFFNNQYG
                     MGGQTSGETMGYDILARVGAGINPEQMHAERVDGFNPLAVIDAIKRKKEVLKGGDGPV
                     LLDTLTYRFSGHSPSDASSYRTKEEVDAWMEQDPLEVYKKDLVEAGIAREDEFNAIWS
                     ETMELITKTLGLAIDDNISPHMDLRSNPNIIGDLMFSNQRIEKMEDREPDVLIPKEDN
                     PRLKQIEKKVRTAYQDGKPVPKNRVFQFRDAVFESLLDKFYLDPTLVAYGEENRDWGG
                     AFAVYRGLTESLPYHRLFNAPISEAAIVGSAVGYALSGGRVVAELMYCDFLGRAGDQV
                     FNQLAKWQSMSAGVLKMPVVLRVSVGSKYGAQHSQDWTSLCAHIPGLKVVFPATPYDA
                     KGMMNSALMGTDPVVFFESQRIYDMGEMFNPNGVPEEYYEVPIGEPDIKREGEDITIL
                     TVGATLYRAIEVADRLADDYGISAEIIDARSIVPFNYEGVIRSLQKTGRILLASDACE
                     RGSYLNDLAQNISELAFDHLDAPPVVLGARNWITPAFELEEHFFPQPDWFIDIIHERI
                     LPLRGHTVGGNFSRDEQIRYNKYGV"
     gene            complement(7152..7844)
                     /locus_tag="VFD89_04515"
     CDS             complement(7152..7844)
                     /locus_tag="VFD89_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZJ57485.1"
                     /translation="MKVSRSARVSESIFSRLWHRYWHFSLLSLYGVCQIWFYYLERVI
                     RPEYFMRSVIDNYIPFVRVFVLPYLFWFAYMAIGFLYLGFVSKKDYFRLCWFIFGGMC
                     ICYVLYMMFPNAQNLRPDITTNDVFSRMIRHIYETDTNTNVAPSIHVLNSIGVHVALI
                     NCEEFKEQSKVWRLLSFISAVLISLSTVFIKQHSIKDVMWAMLLAMVLYLAIYAIPRY
                     LAEGDIFATDTK"
     gene            8123..8782
                     /locus_tag="VFD89_04520"
     CDS             8123..8782
                     /locus_tag="VFD89_04520"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HZJ57486.1"
                     /translation="MYIVLLGPPGSGKGTQGHLLSRQLEIPHLSTGDLFRDILGNPNH
                     ELYKSMQVIEEGKLVSDDVVNRVVGDSINSPRYKDGIIFDGYPRTVAQARALDQILGD
                     MGKKVDAVIDIHVTKEVLLGRLLGRRSCPNCKEIFHISQGIKKCPYCHITLIQRADDN
                     QETILERFGEYQSKTAPLQEYYRHSHSNYIVIRVSDSSRTPANVQREVVDSLYGLGLA
                     K"
     gene            8782..9489
                     /locus_tag="VFD89_04525"
     CDS             8782..9489
                     /locus_tag="VFD89_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZJ57487.1"
                     /translation="MKIYAIADLHLPGGQDKPMDIFGTHWEGHWEKIKRNWSEAITPQ
                     DIVLVPGDISWAMTLEDALPDLDEIGDLPGTKILTKGNHDYWWSSVSRIREQAQESIH
                     VIQNDSFKPTEMPGLAFCGTRGWIAPGARGFTEHDEKIFHRELNRLRLSLDSAAEAHE
                     IIVLLHFPPFDDKGRENEVFHLIRQYPVRHVIFGHLHGDGLRGVVEGEMDGLMYHLVS
                     CDYLDFRPKLIEAKDEN"
BASE COUNT         2795 a         2062 c         2433 g         2445 t
ORIGIN      
        1 cttttttaac atcccctttt cccccttaca tgaaaaacgg cagcatgttt gcccttgtca
       61 aacttccgcc gcttcttttg ttttgttaaa acatcggaag caattgcatt ggcaattgca
      121 acacacaggt ttcaacggga cgggtgattt gtactcacca cctgttttac taatgggaac
      181 tcgccgctta tagaagcggg agacatcttg ccgactcgtt ataaaagtgt cataaggcag
      241 cgcgttatgt cggtccatta tttatgtaat gggctattga aaggcggatg gcttcatcga
      301 ttttggttat tatttggcgg attattcctt gtaatgcagg atatgaatgt tgtataataa
      361 atccatgggt tttacttaaa gtatagtgga tttcttaagt ataatgggct ttgtatcttc
      421 agatgcttca aaaaaaatga aaagcctttt ttcataacgt atataaagga ccaaagcata
      481 ttagtggagg gatataatga aaacaagagc agtcaggtta tacggtgtaa acgaccttcg
      541 tctggaagaa ttcgaattgc cggccatcaa agaggatgaa atcttagcca aggtaatatc
      601 cgacagcgta tgcatgtctt cttacaaggc cgctattcag ggagccaacc acaaaagggt
      661 cccggatgat gtagcggaaa atcccgttat gatagggcat gaattgtgcg gaatcattct
      721 ggaggtgggt aaaaaatggc aggaccaatt cacgtcgggc caaaagttta ccatccagcc
      781 ggccctaaac tataagggca gcctggatgc gccgggatat tcctatccct atataggcgg
      841 tgccgccacc catattataa ttccaagcga agtgatggag acgggcagcc ttttagagta
      901 taagggggat gcttactttt atggatcatt ggccgagcct atgtcctgta tagtaggtgc
      961 ctttcatgcc agctaccata ctacgccggg caaataccat catgatatgg ggatagtcga
     1021 aggcggtaac atggctatct tggcgggagt aggacctatg ggcctaggtg ccatagacta
     1081 tgccattaac tgtgacagaa gacccggact cttggtagtc acggacatag acgaagccag
     1141 gctgaaaagg gccgccgaag ttataacggt ggaggaggca gctgaaagag gggttcggct
     1201 ccattatgta aatacagccg ggcttaataa tccggtggat catctcatgt ccttcaccga
     1261 cggaaaagga tacgacgatg tgttcgtata cgcccccgtc agggcggtca ttgaacaggg
     1321 tgatgggatc ttgggaaggg acggctgctt aaacttcttc gcaggaccta cgaacccgga
     1381 ttttaaagca ctttttaact tctataatgt acattatgcg tccacccaca ttgtaggtac
     1441 cagcgggggt aacaccgacg atctatccga gtctttggat atgatggcca agggcctaat
     1501 agatccctcg acgatgatca cccatatagg gggcctcaac agcgtggtag agacgacgct
     1561 gaatcttcct aatatccctg ggggcaagaa attaatatat acccacatcg atatggatct
     1621 tacagccatt gaggacttca gggaaaaggg gaagacagat caaaggtttg aagatctggc
     1681 aaaaatagtc gaaggaaata acgggctttg gtgtctagat gcagaagaat atctcctgaa
     1741 taatctttga gattgctata tatgaagtac caatatatta tcttccggcg gattaaaaaa
     1801 ggtggcaccc caacggggtc cacctttttg ttatctgttc taacccattt taacccgttt
     1861 taattcattt aaaatccaat atctaattaa cccatctcct ggccaccggt tacgttgatt
     1921 gcctggcctg tcatatagcc ggcctggtcc gaggccagaa agacaaggac gtttgccaca
     1981 tcttcgtatg tacatccccg tcccaggggc acctgattga gatatttttg tctgacctcg
     2041 cctttggtga tgccctggtt tcgggcatat tgttcgtata ggctgtcgac ccaaagaggg
     2101 gaatccaaga ggttaccggg acagattgcg ttgaccctga tatttttggg agccaattcc
     2161 agagcaaggc tttgggtaaa cccgataccg ccgaacttac tggcggcgta agcagagttt
     2221 ttaaaagaac cctttttacc tgatttgcta tttatctgta ttatattgcc ataaccctgg
     2281 cttaacatta cacctgatac cgcctttgca caattaaaga atcccaaaag gtttacttcc
     2341 ataacctttt tccattcttc gctggtaaat tcgtctatgc ccttagctat gagaatggcc
     2401 gcattacaga ccaacaggtc aatccggccg tatttggcca tggtctcatt taccatgttc
     2461 tcacactggg aataatcgga aacatccacc ttgacagcca tgctgcccgt gagttgattt
     2521 gcggtatccc aagccgcatc cgagttgatg tcggctataa cgacgtttgc cccttcattg
     2581 tgaagcctct gacatagagc ctgtccaagt ccctgaccgc cgcctgttac tatggcgatt
     2641 tggccttcca tcttgcccaa gagaatcacc ctttctttac aataattgta caatattcca
     2701 agcccaaaat caataacatg gtttgtcctg catagggcaa cttaagataa tagatgaggg
     2761 gactaagttt atccccatgc caattgtggt ggatttatat tatcctatga tgataaattt
     2821 tatagtcaaa gctatttata tataaatggg tttatcatgc taatttgggc ccatggggca
     2881 tagcaacagt agcacccggg aggcggccaa ataaattgac tcaaaagggt taatctggta
     2941 gaatatttgt gtataacaca tcgttgtaaa gcctaaaaac agcacatgtt tggacatact
     3001 ataaataatc aattggagga aatctatatg gcgataccgg taattatgcc ccgtcaggga
     3061 cttagcgtag agagttgcat tatcaccaaa tggcataaag agaaaggtga tgcagttgag
     3121 gcaggggagc ccctttttac atatgagacc gataaggcca catttgagga ggaatcccag
     3181 cattcaggcg tgctgttgga catttttttc gaagagggag acgatgttcc ggtacttacc
     3241 aacatctgtg tcattggcca ggcgggggaa gatccatccc aatactcgcc ccacggggaa
     3301 gcggcggata gtttggcctc gaaggagcac acggaaggag aaccggtccc tatcccggag
     3361 acagggccgg aggataccga atcgtctacg gatgtaccac caataatcga tcaaaatgac
     3421 gatattataa gggtatcgcc cagggcgaag aatttagccc ataaggccgg gattgatatt
     3481 accaaggccg aacccaccgg ccccgaggga cgtataattg aaagggacat attggccctt
     3541 atggataaga aactgccgct ggcaccgccg gtaaccgcag aacttgatac cgagcccggc
     3601 tatgtggagg aggggcttac caatatacgc aaggtgattg caaggacgat gcatgaatcc
     3661 ttgatgacat ctgcacagct aaccctgcat tcatcctttg atgcgacgga aatactggcc
     3721 tttaggtccg atttaaaaaa acacggcgaa aggttgggcc ttggccgaat aaccctcaac
     3781 gatatgattt tatatggggt ttcccgaacc ctcttaaacc ataaacactt aaatgctcat
     3841 tttctcggcg attccatacg aatatttaaa acggttaaca tgggggtagc catagacacc
     3901 cccaggggcc tcatggtacc caccgtattt ggcgccgaca gtaaatcttt aaatcaaata
     3961 tcccaagagg taaaacaatt aaacgaagcc tgccaagcag ggaccattaa cccggatatc
     4021 ctaaccggcg gatcctttac cgttacaaat ctgggtacct tagggataga atcatttact
     4081 cccgtcttaa acacacccca gacgggcata ttgggcgtca acaacatagt gcaaagggcg
     4141 agggaatccc atggacaaat tgaatactat ccggctatgg gcctctcatt gacctttgac
     4201 cacagggcag tagacggagc accggctgct aggttcttaa aggagctggt ggaaaacctt
     4261 gaaagcttta gactgcttct ggctaagtag ttattgatag attaaaattt gaaaggaaag
     4321 gggtatgttc atgccaaaat cccagtttat cgatcccaat gaggtccgaa aaagtggctt
     4381 cattgagctt gacgatattc cgataaatca atacaaaagg acgataacac aggagaagga
     4441 caactactca aaagaggact ttatgaggat atttcgggat atggccatta tccgcgaatt
     4501 tgagaccatg ataaacgaga taaagaccat gggccaatat aacgggatag agtataacca
     4561 cccggggcct gcgcacctgt ccataggcca ggaggcatcg gccgtaggac aggcgtacct
     4621 attggaccag caggatttta tattcggttc ccacagaagt cacggcgaaa tcctggccaa
     4681 gggtctgtcg gccatctatg ggatggggga cgaagactta ttaagcgtta tggaagaatt
     4741 ctttgacggg agcatcctga ggatagtgga aggcaggcaa aaaaatgcag gcagtgtaaa
     4801 ggaattggcc atagattttc tcttatacgg taccttggcg gagatctttg ccagagaaac
     4861 tggctttaac aaggggcttg ggggctcaat gcatgcattc tttacgccct ttgggatcta
     4921 tcccaataac gcaatagtgg gtggttccgc cgatattgcc ttgggctccg cactatataa
     4981 gaagataaac caaaaaggcg ggatagtcat tgccaatata ggagatgcat ccatgggctg
     5041 cgggcctgtg tgggaggcta tgaacatggc tgccatggat cagtttaaac tcctatggga
     5101 cgagagccat aggggcgggc tgcctataat attcaacttc tttaacaacc aatacggtat
     5161 ggggggacag accagcggcg agaccatggg ctatgatatc ctggccaggg ttggggctgg
     5221 cataaacccg gaacagatgc acgcggaaag ggtggacggc tttaacccct tggctgtaat
     5281 cgatgccata aagagaaaga aagaggtcct aaaaggcggg gatgggcctg tcctactgga
     5341 taccctcacc tatagatttt ccggccattc cccctctgat gcttccagct atcgcaccaa
     5401 agaggaagtg gacgcctgga tggagcaaga tcccctcgag gtctacaaaa aggatctggt
     5461 ggaggcgggt atagcacgtg aggatgaatt taatgctatc tggtcggaga ccatggaact
     5521 tattacaaag actttggggc tggccataga cgataatatt tcgcctcata tggaccttag
     5581 aagcaatccc aacataatcg gggatttgat gttctccaat caaaggatag aaaagatgga
     5641 ggacagagag cccgatgtac ttatacccaa ggaggacaac ccgaggttaa agcagataga
     5701 aaaaaaggtg aggaccgcct atcaggatgg gaaacccgtc cctaaaaaca gggtatttca
     5761 attcagggat gcagtttttg agtccctgct ggataagttt tatctagacc ccaccttggt
     5821 tgcctatgga gaggagaaca gggattgggg cggagccttt gcggtataca ggggccttac
     5881 cgaatctttg ccctatcata ggctgttcaa cgcccccatc tctgaagcgg ctattgtggg
     5941 ctctgcggtg ggatatgccc tttctggggg cagggtagtg gcagagctta tgtattgcga
     6001 ctttttaggt cgtgccgggg atcaggtgtt caaccagctg gctaaatggc agtccatgag
     6061 tgcgggggtt ttaaagatgc ccgtggtttt gagggtatcc gtaggttcaa aatatggggc
     6121 tcaacattca caggattgga catccctatg cgcccatata cccggtctta aggtagtctt
     6181 tcccgcgact ccgtatgatg ccaagggcat gatgaacagt gcgcttatgg gaacggatcc
     6241 cgttgtgttc tttgagagcc agaggatata cgatatgggt gaaatgttta atccaaatgg
     6301 ggtcccggaa gagtactatg aggtccccat cggcgaaccg gacatcaaaa gggagggcga
     6361 ggacataacc atattgaccg ttggggcaac cctgtacagg gctatagaag tagcggacag
     6421 gctcgctgac gattacggca taagcgccga gataatagat gcaaggtcca tagtcccctt
     6481 caattatgaa ggggttatcc ggtcccttca aaagaccgga agaatcctat tggcaagcga
     6541 tgcctgcgag aggggatcgt acctgaacga tttggcccag aatataagcg agctggcctt
     6601 tgaccatctg gatgcccctc cggtggtact tggggcaagg aactggatta cccccgcctt
     6661 tgagcttgag gaacatttct tcccgcagcc ggattggttc atagatataa tccatgaaag
     6721 gattctgccc cttagagggc ataccgtcgg cggtaacttt tcaagggatg agcagatcag
     6781 gtacaacaaa tacggggtat gacgaatatt aaaaggggtg cacaatgtgc acccctttat
     6841 tgcagattca gtcaaaacga aaattcaacg cataatggga tagaggtaat gaactattaa
     6901 aaagcggatg acttcattga tttggttaag aaaacttggc ctatggaacg cagaaaaggc
     6961 gtagcttaag gcaggacgcc ttaagccgat tccaccggca ggaagccgga ttggaagcga
     7021 taggcttttc aagtggaata ggccttagtt ttttccaaat caatgtcgga agtagcgttt
     7081 aatagtcatt accgacatgt ttaactggta caatgtgata aaggtaatga actattgaaa
     7141 agtgtatgac ttcatttggt atccgttgcg aagatatccc cttcagcgag gtaccttggt
     7201 atggcgtaga tggccaggta taataccata gccaggagca tagcccacat aacgtccttt
     7261 atagaatgct gtttgataaa cactgtggac aggctgatta gcactgcgct tataaaggac
     7321 aacaagcgcc aaaccttgga ttgctcctta aattcctcgc agtttatcaa tgcaacgtgg
     7381 accccgatgg aattgaggac atggatgctg ggtgccacgt tggtgttggt atccgtttcg
     7441 tatatatgcc ttatcatcct ggagaatacg tcatttgttg taatatcggg cctgaggttt
     7501 tgggcatttg gaaacatcat gtacaataca taacagatgc acattccgcc aaagataaac
     7561 caacatagcc tgaaatagtc cttcttagag acaaacccca agtataaaaa ccctatcgcc
     7621 atataggcga accagaaaag atagggcagg acaaaaaccc taacaaaggg tatataatta
     7681 tcaataacgg aacgcataaa gtattccgga cgtataaccc gttccagata atagaaccag
     7741 atttggcata ccccatatag ggacagaagg gaaaaatgcc aatacctgtg ccataaccta
     7801 ctaaatatac tttcactaac ccttgcactt ctgctaacct tcaccaatat aacccccact
     7861 ttaactttta aagactcatt tgtatataat ccgataatgt aaatttccgt acgccataat
     7921 tatacaataa attgtgttat acaaatctta aaagtgtctt aagaagccat atttaggatg
     7981 gggaccctgg tgtcccatgg gttttaactt cgtggcttgg gcataaattg ggccatggga
     8041 tggataagtc ccggttgttt atgaagtttt tctatgctag aattagtagg gaaagaaatg
     8101 gctctacgag gaggaattta ttttgtacat tgtattatta ggacccccgg ggtccggaaa
     8161 agggactcaa ggacatctat tatcacggca actagaaatt cctcaccttt caacgggtga
     8221 tttatttaga gatatcctgg gtaatcccaa tcatgaacta tataaatcca tgcaggttat
     8281 agaggaaggt aagctggtat ctgacgatgt cgtaaatagg gtggtggggg atagtatcaa
     8341 cagtcccagg tacaaagacg ggattatttt tgacggatac cccagaaccg ttgcacaggc
     8401 ccgggccttg gaccagatat taggggacat ggggaaaaaa gttgatgcgg ttatagatat
     8461 ccatgtaacc aaagaagtgc tcctgggaag actgctgggg cgcaggagct gcccaaattg
     8521 caaggagatc tttcatataa gccaaggaat aaaaaagtgc ccctattgtc atataaccct
     8581 catacaaagg gctgatgaca atcaagagac tattttggaa cgcttcgggg aatatcaatc
     8641 caaaaccgcc cccttacagg aatattacag gcatagccac tctaattaca tagttattag
     8701 agtctccgat tcgtctagga ctcccgcaaa cgttcagcgg gaagtggtgg atagcctata
     8761 tggattgggg cttgcgaaat aatgaaaatc tatgcgattg ctgatttgca ccttccgggg
     8821 ggacaggaca agcccatgga tatctttggg acccattggg aggggcattg ggaaaagatc
     8881 aagagaaatt ggtcggaggc cataacccct caggacatag tcctcgtgcc gggggatatc
     8941 agctgggcca tgacattgga agatgcgcta ccggatttgg atgaaatagg tgatttgcct
     9001 ggcaccaaga tattgacaaa ggggaatcat gactattggt ggtcttccgt ttcccgcata
     9061 agggaacagg cacaagaatc aatccacgtg atacaaaatg acagctttaa acccacggaa
     9121 atgccggggc tcgccttttg cggtaccagg ggctggattg cacccggggc taggggattc
     9181 accgaacacg atgagaagat atttcatagg gagctcaaca gattaaggct gtctctagac
     9241 tcagctgcgg aggcccatga gattatagtg ttgctgcatt ttccgccctt tgatgataag
     9301 ggtagggaaa atgaagtttt ccaccttata aggcaatacc ccgtaagaca tgttatattc
     9361 ggccatcttc acggagacgg tttaaggggt gtagttgagg gggagatgga tgggctgatg
     9421 tatcatctgg tatcctgcga ttatttggat tttagaccaa agcttataga ggctaaggac
     9481 gagaactaat ataaggctat gaaaagatca atgatcccgg agaaaaaagc ctataaaggg
     9541 aaccatggct tcggacagaa aaagccaaat tacacaaaga atgaagtcga atagagatta
     9601 tatgtataag aaacgcggaa gaaagccgcg tttcttagtg ttttttggga cttagttccc
     9661 atttttggat tagaggatat tttttttaac aatctgggag gaatttttga aatgatagag
     9721 aatatgtaat tatag
//
LOCUS       DASRNX010000114        21935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_710528,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000114 DASRNX010000000
VERSION     DASRNX010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 21935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21935
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710528"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            694..2190
                     /locus_tag="VFD89_04530"
     CDS             694..2190
                     /locus_tag="VFD89_04530"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HZJ57488.1"
                     /translation="MRLCSFSETYHMFDVTPVENLFIQEFMLKAPGDFVKVYIYGLKQ
                     CYHLSQSENTVDSFSRALCLEAKVVENAFYYWERQGIMEVKKDDGGQLSIEYLNIKDM
                     LYNSCQHDNKSLYHYKDFNQNLQTIFGKRLLTPQEYLRVYDWIEILLLPAEVVLMMVQ
                     FYLSKKSSRLSINYLDKVAESWAKDGINTLQKAEEYIEQYESCYQETIAVLKYLGIHR
                     TPSKAELDLYKKWQDLWGFPLNAVLEACKETTKIQSPNMGYLDKILENLHSLGLLTQQ
                     DIKSYFSSRDTINDSIKDVLFNLGYKSTAPTPEHQSLYIKWTTSWQMDHDVILLACKQ
                     LIRKKTRIGFSQVDGLLAKWKERGFTRSKDIKNYLRHKKILINEIRTVLERAGDNREV
                     TTADQRVFIRWTKEWNISYELILLAAEYSTMAENKLPFINKILNNWYDSGITSVKEAK
                     ADHERHTQGGQGIGSESAMRKQVDFNQFEQHSYTDEDLEHLFEDLENV"
     gene            2209..3219
                     /locus_tag="VFD89_04535"
     CDS             2209..3219
                     /locus_tag="VFD89_04535"
                     /inference="COORDINATES: protein motif:HMM:NF005304.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ57489.1"
                     /translation="MSDIYLKEILREYDEIQNAEYRALGLRRQEIFDNIPDIAKIHND
                     IVELMAQNSRKVILSSDSDPAALMTELQHRIDRLKAQEIDLLKQNGYPEDYLEMQYRC
                     ENCKDTGYTGFPIKEKCNCLTQKLLERTYRLSNIQELEAENFQTFDPMVFPEVPLKNS
                     RLNQREYMTGLRDRLIDYIAEFPNNEKRTILFTGKTGLGKTFLLNCMAKSIMDNGYTV
                     IRISAYKLFDQLFYSAISDNTDNLAQKRSLFDVDALIIDDLGTETRRNNFTIEDLFNI
                     INERTLKRTHTFLSTNLGLSNLSQRYTDRIASRLFDSSNTMIIRFQGMDIRLKNKAHQ
                     YS"
     gene            complement(3207..4544)
                     /gene="dnaB"
                     /locus_tag="VFD89_04540"
     CDS             complement(3207..4544)
                     /gene="dnaB"
                     /locus_tag="VFD89_04540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZJ57490.1"
                     /translation="MEGLSRMPPHNMEAEQSVLGCMLLDKESVAAATDFLRGEDFYAE
                     SHKEIFESMLDIYDHGEPVDLVTVTEQLRQRGTLEAIGGVAYISDLSMAVPSTANIRY
                     YVGIVEEKSILRRLISVCNGIIKESYEAREEIDLIIDHAEKSIFQITQRNTSGDFEPI
                     KTVLLEAYARIEELSKNKGQIIGIPTGFTDFDQKTSGLQPSDFILVAARPSMGKTSFV
                     LNVAQYAALHADVPVAIFSLEMAKEQLVQRMLSSESNVELQKIRTGELDENEWIKLVE
                     AAGPLSQAPIYIDDTPGITAMEIRSKARRLKIEKGLGMIIIDYLQLMSGRGRIENRQQ
                     EISEISRSLKALARELSVPVVTLSQLSRAPEARTDHRPMLSDLRESGAIEQDADVVAF
                     LYRDEYYNPDTDKKNIAELILSKQRNGPTGTVELVWLGQFTKFANYDRTHQEY"
     gene            complement(4563..5009)
                     /gene="rplI"
                     /locus_tag="VFD89_04545"
     CDS             complement(4563..5009)
                     /gene="rplI"
                     /locus_tag="VFD89_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZJ57491.1"
                     /translation="MKVILLKDIKGSGVKGDVINVSDGYARNYLFPRGLATEATKANL
                     SELENKERAEEKRREQAAEQAREMANRLSDITLVLKAKSGENGKLFGSVTNKEIAQEL
                     KKQYKISIDRKKIVLGEPIKQLCEMELEVKLYPEIGGKLKIKVEEA"
     gene            complement(5006..6580)
                     /locus_tag="VFD89_04550"
     CDS             complement(5006..6580)
                     /locus_tag="VFD89_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HZJ57492.1"
                     /translation="MKRWSWLRRIFGFLGVSEHQETAEAEPPKPAVQTLVQKEHREIQ
                     LVIAHIYVDNYDEVLANTEDANKPLLIAEIEGRLGKWAMNHSAGWQKYDRDKFLMVMD
                     VNILEALEGNRFGILDDIREIDMGSHVPPTLSIGVGVEAQSPSQSIDFAKTALDMALG
                     RGGDQAVVKKGTKLYFYGGKTKAVERRTKVKSRVMAGVLRELMEQSYNIFIMSHELPD
                     LDSIGSALGLYRCASHIGKEVYIVLKESNASIEYLMEELKRKKEYENLFISPRDAESK
                     IDGYSLLIVVDTHRPSFTEAPELLDIADRIAVIDHHRRSAEVIENPALAYLEPYASSA
                     SELVSEMVQYFDEKLRLDPLEADALLAGITMDTKSFTFKTGVRTFEAASYLKRSGADP
                     TSARQLYKDDIETYIARAETVKKARILAPNIATSQCPPGVKNPSLIAAQAADSLLTIR
                     GIEASFVLGQMDGGVIISGRSLGRINVQLILEKLGGGGHMTIAGAQLKDITIEEAGRR
                     IASVIQEYLNEEGESK"
     gene            complement(6599..7576)
                     /locus_tag="VFD89_04555"
     CDS             complement(6599..7576)
                     /locus_tag="VFD89_04555"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HZJ57493.1"
                     /translation="MEKPAKAILEGIVVGVIITLLTMVLYAVPLFNILILILPVPIVV
                     IGIRRGTLAGILSLVVPPILIGLLGDPLLGIIMLCLSLFVVLGLTIGYKRDLGMNESV
                     ILSAGGVLLSVLLSLQVFSWMMGESFFDFLWKRMGDLLNSGIVDLKAIREFYHALGIV
                     DKAYPADKFIEFLLSQMKEVVPLFPAMLLITSLFVGGLNFLISRWILKRLGSQTPKVP
                     PFKRWNLPRGTGRGFLGLMLVSAIGSWVKIPNFDVVLYTISAVFTFIFTIQGLSVATF
                     FLGERRVPGIVGVLILIATLVFLSFALTFLGIFEQIFGARKAYDSRRGD"
     gene            complement(7600..7935)
                     /locus_tag="VFD89_04560"
     CDS             complement(7600..7935)
                     /locus_tag="VFD89_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HZJ57494.1"
                     /translation="MARGKAGDFDITRNIRLIEWLKSEMLNSIAKVFSLLAGGTRSSQ
                     EVLIGYLGDVIVACYLLGKRLGIHYALLDQTIEDQIRLGIIEGNDIEKSYGDLSELQR
                     HLKESRGNP"
     gene            complement(8025..8852)
                     /gene="pheA"
                     /locus_tag="VFD89_04565"
     CDS             complement(8025..8852)
                     /gene="pheA"
                     /locus_tag="VFD89_04565"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HZJ57495.1"
                     /translation="MKQIGYLGPVGTFTHDAALIYTKGCKYRLIEFSSIQELILAVSG
                     GEVDEAVVPMENSMEGTVNITVDMLIHEVDLNVAGELIIPICHCLMAKGGTKIADLTK
                     VLSHPQALAQCRRYLDNLATGIKREVTESTAAAAMLVMESREPWAAVANYRAAHIYGL
                     DILKEGIQDNNYNSTRFVVLSTNEAEMTGEDKTTLVFTVNHKPGGLFQALKIFADLNI
                     NLTKIESRPMRTLLGEYLFLVDLEGHASEDKIGIALNRLRKQCRFFKMLGSYPKIYP"
     gene            8975..9646
                     /gene="yunB"
                     /locus_tag="VFD89_04570"
     CDS             8975..9646
                     /gene="yunB"
                     /locus_tag="VFD89_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HZJ57496.1"
                     /translation="MRKFKSLRMAKIGIFGVIAILLGLALLIFLFIDYNINPAIITFS
                     QAKIQAIAINSLSDAVKESLGNNIKYTDFVNVLTDKNGNVAMLQANTMKMNDLAAQVS
                     DMAQETIRSKGDEGIDIPLGTITGSKLLAGMGPKIRVKIISFPAVSTDFDSELESSGI
                     NQTRHKIYLTLRARIKIAIPLNTADIDIDSRVPVTETIIVGDVPQTYINVDDTDKMLK
                     LVPTD"
     gene            complement(9944..10195)
                     /gene="rpsR"
                     /locus_tag="VFD89_04575"
     CDS             complement(9944..10195)
                     /gene="rpsR"
                     /locus_tag="VFD89_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZJ57497.1"
                     /translation="MAEERRRPRGGGQRRSRRKACSFCIDKVDTIDYKDAYKLRKYIT
                     DRGKILPRRISGNCAKHQRKLTAAIKRSRVIALLPYATE"
     gene            complement(10216..10647)
                     /gene="ssb"
                     /locus_tag="VFD89_04580"
     CDS             complement(10216..10647)
                     /gene="ssb"
                     /locus_tag="VFD89_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZJ57498.1"
                     /translation="MLNKVVLIGRLTRDPELRYTSSGIAVTRFTLAVDRNFTNQQGER
                     EADFIPIVVWRGQAESSAKYLSKGRLVAVSGRIQTGSYDTPEGQRRYTTDVVADDVRF
                     LEWGEARTGGDPDVNRAHDDSFGVTPGFQPLENDGDDELPF"
     gene            complement(10665..10952)
                     /gene="rpsF"
                     /locus_tag="VFD89_04585"
     CDS             complement(10665..10952)
                     /gene="rpsF"
                     /locus_tag="VFD89_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZJ57499.1"
                     /translation="MNKYESIYILRPTMEEEAVKAMVERFSNLITAEGGEIEKIDEWG
                     KRRLAYPIDDFREGYYVLMNFNAESQLPSELERNYKITDGVIRYIIIRDDE"
     gene            complement(11067..12047)
                     /locus_tag="VFD89_04590"
     CDS             complement(11067..12047)
                     /locus_tag="VFD89_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HZJ57500.1"
                     /translation="MDKKNYAVFIDLENTGGNVNTLKKVIEKVKIRGDILIGKVYGYQ
                     ECFSGLKETLLSNTFNVLPSIRYGVNQKNNLDIQLVIDALEVAYTNPLIDCFCIVSGD
                     SDYTPLVGKLKSMGKFVLGISRSEVASNIFIKACNEFIFLETVTTGKRGNLQKEDDAV
                     GTQKELVEVVKKIIDDQADADGTMFVSELKKTLLRLKPDFSEKNYGYNSFGRLLNNLE
                     YKYGVIRLFGEYNSLKVKLKIENPIRTGEINRVNWAAIFQEKLDEFKQNGFKRINPSI
                     IKAEIQNDYPDFDERQIGFKKFSDIMKRLEKDNKISIEFNEAKTMLIKIL"
     gene            complement(12822..14486)
                     /gene="ilvB"
                     /locus_tag="VFD89_04595"
     CDS             complement(12822..14486)
                     /gene="ilvB"
                     /locus_tag="VFD89_04595"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZJ57501.1"
                     /translation="MKLQGAQVLIECLREQGVDTIFGYPGGAVLPIYDALYDVEDIRH
                     ILTAHEQGASHAADGYARATGKVGVVVVTSGPGATNTVTGIATAYMDSVPMVVFTGQV
                     ASSLIGRDSFQEVDITGITHPITKHNYIVKDPEQLAHIIRRAFHIARSGRPGPVLVDI
                     PKDIQVAMVDYIPAPTNKDCGDGTREDGLDENVYRAIEAINDCQRPVIYAGGGINIAG
                     ANERLVEFAERIKAPVSCSLMGMGAFPGTHPYYMGMVGMHGTRYSNYAFTNCDLLIAV
                     GARFSDRVVSDVEKFAPKARILHVDIDPAELGKNVSTHIQICGDVGQVLDFFNEKVKI
                     KEENSWNLQITEWKREHPLTYKMNGSLSPQYIIEKLYDLTDGKAIISTEVGQNQMWAA
                     QFYKFTRARSFISSGGLGTMGYGLGASIGAALGRPGERVVNIAGDGSFKMNSTELATI
                     SRYRIPVIQLVLNNHSLGMVRQWQDLFFNKRYSHTSFGPDVDFVKLAEAYNIKAFRID
                     KNQQVEEVLKNALELNEPVLIECDIHPDDKVYPIVPPGAGIDNLID"
     misc_binding    complement(14568..14823)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14995..16311)
                     /locus_tag="VFD89_04600"
     CDS             complement(14995..16311)
                     /locus_tag="VFD89_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZJ57502.1"
                     /translation="MPLDKIVDFYRGKLIKSTQELVAIPSVKGDPLEGKPFGEGINRA
                     LEYTLELGEKLGFLVGNVDGYAGFIEFGQGSETLGILAHLDVVPEGEGWTYPPYGGEI
                     HDNRIYGRGAIDNKGPALAALYAMKAVMESGVSVNKKVRLILGTDEESGWADMDYYFN
                     KQSMPDFGITPDANYPVIHAEKGIVHIELCKEFNGAGNGERSPILRVKGGSRANMVPD
                     NCTYMLQGKADPINVKGVSAHGSTPAKGENAIAKALKDLWDRGLGNEPIDKFIADLYK
                     LVGMDTTGEGLGIKCRDDVSGDLTLNLGVIHVDSEVGKAVLDIRFPVEYTQGEILLKV
                     HEKLKGTGIDVRVLHGQKPLHVPKDHFLVETLIRVYKEQTGEESAEPLSIGGGTYARA
                     LDTGVAFGAVFPGRPELAHQKDEYIDIEDLVLNTRIYAHAIAELVR"
     gene            16753..17478
                     /gene="nagB"
                     /locus_tag="VFD89_04605"
     CDS             16753..17478
                     /gene="nagB"
                     /locus_tag="VFD89_04605"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HZJ57503.1"
                     /translation="MKIIISESREEFNGTAADIVRRQIQDKPDSVLGLATGSTPLGLY
                     NLLVCMGIKGDLDFSRIKTFNLDEYKGLSRDHPQSYYHYMYENLFSKINIRDENVNIL
                     NGEKADSYRECAEYEKRIKASGGIDLQVLGIGHNGHIGFNEPGTPFDSITDLIDLTDR
                     TIEANSRFFDSPHQVPRQALSMGIKTIMQARNIILLITGSAKARIAAKALRGSITPDV
                     PASVLQLHPFVTVILDKESAAYL"
     gene            complement(17706..19328)
                     /locus_tag="VFD89_04610"
     CDS             complement(17706..19328)
                     /locus_tag="VFD89_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004814333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HZJ57504.1"
                     /translation="MKRIALLLVIIMTFGMLAAGCGASGAKELVVNVGPEPDTIDPAL
                     NSAVDGATLLIHAFEGLMNLDKDGVPVEGQAKSYEVSEDGLTYTFTLRDGLKWSDGEP
                     VTAEDYVYSWKRAVDPETAADYEYMFEVIDGYDAGQDVDEDDEDWVPAHDLNVKALDE
                     KTLEVKLITAAPYFLELCAFPTFFPVRKDIVEADPDGWATKPETYIGNGPYVMDEWVH
                     ESHITYKKNPNYWNKSEIGPEAIKFLLMDDDGAILAAFENGEILLADSMPNDEIEAWK
                     DKDEFFIQGQLGTYYISFNVTKEPLDNPKVRKALTLAIDREFICEKIGQAGQQPAGAF
                     VATGLTDADPTKEFREVGGDYYDPVDYEKNLEEAKQLLAEAGFPNGEGIPTIEYLYNE
                     STGHQQIGEALQDMWKEIGVNIELASQEWNTFLNTRKNGEYYIARNGWLGDYNDPISF
                     LDMWITGGGNNDAQWSNKEYDSLISQVKASSDREERMKLMHQAEDIIFEESMLAPIYY
                     YVDIYLKSPKLEGFYSSPLGYKYFMFSKVVDK"
     gene            complement(19461..20474)
                     /locus_tag="VFD89_04615"
     CDS             complement(19461..20474)
                     /locus_tag="VFD89_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ57505.1"
                     /translation="MEKHLDEGRLDIKASEPPLIEVKNLKQYFPISDGLFRTKYVKAV
                     DDVSFYIKKGETLGLVGESGCGKTTTGRSLLRLYEPTDGRIIYDGVDITHTDMMPYRR
                     KMQIVFQDPYASLNPRMTVGDIVGEPIDIHKLAHSKKERTDRIIEMLDRVGLNSEHAN
                     RYPHEFSGGQRQRIGIARALAVDPEFIVCDEPVSALDVSIQAQIVNMFEELQEDMGLT
                     YLFIAHDLSVVKHISDRIGVMYLGKLVELADSYELSFHSVHPYTRSLISAIPVPDPDA
                     SRQRQRIVLEGDVPSPLDPPSGCRFRTRCPYADELCAREEPEFREVSKGHFAACHHLD
                     RVK"
     gene            complement(20474..21478)
                     /locus_tag="VFD89_04620"
     CDS             complement(20474..21478)
                     /locus_tag="VFD89_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ57506.1"
                     /translation="MSNVLLDIKNLRLSFFTPAGEVKALNDVTFKMNEGDVLGIVGES
                     GSGKSVTAYSIMGLVPFPGRIIGGTIDFNGHRINDMTEKEMREIRGNEVSIIFQDPMT
                     SLNPVYTIGNQIREVILLHTDMDNEQAHKRSLDLLTLVGINEPEKRLKQYPHELSGGM
                     RQRVMIAIALACEPKLLIADEPTTALDVTIQAQIMELMMELKEKINMSIIIITHDLGV
                     VANMCQKIVVMYGGKIVESGTSDEIFYNPQHEYTKGLLRSIPKINTKSHEKLIPIEGT
                     PVDMLNPPAGCPFAPRCDKCMKICLREMPEYTKISDTHDCACWLLQKEAFKAAAGGEG
                     "
     gene            complement(21491..>21935)
                     /locus_tag="VFD89_04625"
     CDS             complement(21491..>21935)
                     /locus_tag="VFD89_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZJ57507.1"
                     /translation="INIVGTGLISIFIVFSLLYWVGMARIVRGQILTLKEQEFVTAAR
                     ALGASHSRIIKKHLLTNCVGTLTVTTTLQIPSSIFTESFLSFLGLGVNAPMPSLGSLA
                     AQAVDGIRSYPHRLLAPAVAISLIILSFNLFGDGLRDAFDPRLKN"
BASE COUNT         5821 a         5426 c         4378 g         6310 t
ORIGIN      
        1 cctttcaatg ctagataaat gacgatggtc agatagtaag ttggaactag tggtggtcta
       61 ttagatgtct atatcaatca aagggtcggg cctttatttg ttaggtcgga aaccaagatt
      121 ttcccgttta attggttgaa aacacaaaaa aaatcggtca aatgacatat atgtgtacat
      181 tgggtgttgt agctgcaggt ccaaaatcta atgaactatc tagataatat cgtcaacgca
      241 ggcgtatgag gaatcttatg aaaaagttta aatacggtta tgtagtcatt tccataggta
      301 tcattgataa aaggcaataa atgctaaatg tttttctaaa atccatatat ttagatgtat
      361 aatgtactat gtgttattga ttttatatta acattgccca tattatctgt caagcacaaa
      421 ttggccagat tttggtatgc cgtattatgg tcccatgtga caatgacgac tatacgagat
      481 ggcacgaggt ggtaaaataa tcaaaaattc ttttgtttcc ggttggaagc aattgaatca
      541 taaaccctct ccgaacattc gacatctttt ttgcctttcc atctccaaat agggtgtttg
      601 gcaggttgct tttctccttc tttatgatat cattaattct ggggagataa catcttttcc
      661 ccacaaaagt gaattctgat aaggatgata tgtatgaggt tatgctcttt ttcagagacc
      721 tatcatatgt ttgatgtaac cccggtggag aacttgttca ttcaagagtt tatgctcaag
      781 gcaccggggg attttgtcaa agtttatatc tatggtctta aacaatgcta tcatttaagc
      841 caatccgaaa ataccgttga tagcttttcc agggccctgt gtctggaagc caaagtggtt
      901 gaaaatgcct tctattattg ggagaggcag gggataatgg aggtaaaaaa ggacgatggg
      961 ggccagttat ccatagaata tctaaatatt aaggatatgt tgtataacag ctgccaacat
     1021 gacaacaaat ccttgtacca ttataaggat tttaaccaga atcttcagac catcttcggc
     1081 aagaggttgc tgacacccca ggaatactta agagtctatg attggataga gatcctattg
     1141 cttccggcgg aggtagtgct gatgatggtc cagttctatc tcagcaaaaa gagcagcagg
     1201 ctcagcatca actatttgga caaggttgcc gaaagctggg ccaaggacgg tataaacacc
     1261 ctgcaaaagg ctgaggaata catagaacaa tacgaatctt gctatcagga aaccatagcc
     1321 gtattaaaat atttggggat tcatcggacc ccctccaagg ccgaattgga cctatataaa
     1381 aaatggcagg acctttgggg ctttccctta aacgccgtct tagaagcctg taaggagaca
     1441 acgaagatcc aatcccctaa tatgggatac ctggataaga ttctagagaa cctacatagc
     1501 ctggggctat taacccaaca ggatattaaa agctactttt cttcaagaga tactatcaac
     1561 gacagcatca aggatgttct ctttaactta gggtacaaga gtactgcacc tacccccgaa
     1621 catcagtccc tatacataaa gtggaccacc agttggcaaa tggatcacga cgtaatactg
     1681 ctcgcctgca aacaattgat acgaaagaag actaggatcg gattcagcca agtggacggg
     1741 ctcctggcca aatggaagga aaggggcttt acccggtcta aggacattaa aaattatctc
     1801 aggcataaga agatcctgat taacgaaata aggacggttt tggagagggc cggggataac
     1861 cgggaggtta cgacagccga ccaaagggta tttataaggt ggactaagga atggaatatt
     1921 tcctacgagt tgatcctctt agccgcagag tattccacca tggcagagaa taagctgccc
     1981 tttataaaca agatattgaa taattggtac gatagcggga taacatccgt gaaggaggcc
     2041 aaagccgatc atgagcgaca tacgcaaggc ggtcaaggta taggctccga atccgcaatg
     2101 agaaaacagg tggactttaa ccagtttgaa cagcattcct ataccgacga ggacttggag
     2161 catctttttg aggacttgga aaatgtatga attaggaagt aggtatatat gagcgatatt
     2221 tatttaaaag aaattcttcg ggaatacgat gaaatccaaa acgcagaata ccgtgcactg
     2281 ggtctacgca ggcaggagat attcgataat atcccggata tagccaaaat ccataacgat
     2341 atcgtggaat tgatggccca aaattccaga aaggttatcc tatcctccga cagcgatccc
     2401 gctgccctga tgaccgaatt acaacatcgt atagaccggc tcaaggcaca ggagatagac
     2461 ctcttaaaac aaaacggata ccctgaagat tacctggaaa tgcaatacag atgtgaaaat
     2521 tgcaaggata cagggtatac gggtttcccc atcaaggaaa agtgcaactg ccttactcag
     2581 aaattgttag aaaggacata caggctttcc aacatccagg aattggaggc agagaatttc
     2641 caaacctttg accccatggt tttccccgag gttcccctaa aaaacagtag gctaaatcaa
     2701 agggagtata tgacaggact tcgtgacagg cttatagact atattgccga gtttccgaat
     2761 aatgaaaaga ggaccatcct ctttaccggt aagacgggcc ttggaaagac attcctcctt
     2821 aactgtatgg caaaatccat tatggataac ggctatacag tcataagaat atccgcctat
     2881 aaactgtttg accaactttt ttatagcgcc atatcggata atacagataa cctggcccaa
     2941 aagagaagcc tattcgatgt tgatgccctg ataatagacg atctcggcac cgaaacccgc
     3001 cgtaacaact ttaccataga ggatcttttc aacatcataa atgaaaggac cctaaagcgc
     3061 acccacacct ttttgtccac caatctgggg ctgtccaatc tgagccaaag atataccgac
     3121 aggatcgctt cacggctctt tgatagttcc aacaccatga tcataaggtt tcagggtatg
     3181 gatatcagac taaagaataa ggcccatcag tattcctgat gggtcctgtc gtagttagca
     3241 aacttggtaa actgccccag ccataccagc tccaccgtcc cggtggggcc gtttcgctgt
     3301 ttggacaaaa ttaattctgc gatattcttc ttatccgtat cggggttata gtattcgtcc
     3361 ctatataaaa aggcaaccac atcggcatcc tgttcgatgg ctcccgattc ccgaaggtcg
     3421 ctgagcatgg gcctgtgatc ggtcctagcc tcgggagcac ggctgagttg ggatagggtt
     3481 actacgggca cagacaattc cctggccaac gcctttaggg atctggaaat ttcggatatc
     3541 tcctgttgtc tgttctctat ccttcccctc ccggacatga gctgtaggta gtcgatgata
     3601 atcatgccaa ggcccttttc tatcttcaag cgtcgggctt tagagcgtat ctccatagcc
     3661 gttatgcccg gggtatcgtc tatataaatg ggagcctgag acagcgggcc tgccgcctct
     3721 accagcttga tccactcatt ttcatccagt tcaccagtac gaattttttg aagttctacg
     3781 ttagattcgg aactgagcat acgctgcacc aattgttcct tggccatttc caagctaaat
     3841 atggctaccg gaacatctgc atgcagggca gcatattggg ccacattgag cacaaaactt
     3901 gtcttaccca tggagggcct ggcagctacc agaataaaat ctgacggttg caatcccgat
     3961 gtcttttgat caaaatcagt aaaccccgtg ggtattccta taatctgtcc cttattctta
     4021 gacagctcct caatccgggc ataggcttcc aaaaggacgg tctttatggg ctcaaaatca
     4081 ccgctggtat ttctctgagt gatttgaaat atactctttt cagcatggtc tataatgagg
     4141 tcaatctctt ccctagcctc atagctttcc ttaattattc cgttgcagac agaaataagc
     4201 ctgcgcagga tggacttttc ttccacaatt cccacataat atctgatgtt ggcggtgctg
     4261 ggcactgcca tggacagatc gcttatatac gctacaccac cgatagcttc taaggtgccc
     4321 ctctgtctga gctgttcggt gaccgtaacc aagtccacgg gttcaccgtg atcatatata
     4381 tccagcatgc tctcaaagat ctctttatgg gattcagcgt aaaaatcctc gccccttaga
     4441 aaatccgtgg ccgccgctac cgattccttg tccaatagca tacagcccaa tacggattgc
     4501 tctgcctcca tattatgggg gggcattctg gataggccct ccatcatggt catgtccatt
     4561 acttaagctt cctctacctt tattttcagt tttccgccaa tttcgggata taattttact
     4621 tccaattcca tctcacaaag ctgtttgatg ggctcaccca ggactatctt tttcctgtct
     4681 atggatatct tgtactgttt ctttagctcc tgggctatct ccttgttggt cacggagcca
     4741 aagagtttac cgttttcacc gctcttagcc tttaaaacca aggttatatc cgaaagccta
     4801 ttcgccatct cccttgcctg ctcggcggcc tgctccctgc gcttttcctc cgccctctcc
     4861 ttattttcca attcactcag gttagctttg gttgcctcgg tggcaagtcc cctgggaaag
     4921 agatagtttc tggcataacc gtcgctgaca ttaatgacat cgccctttac acctgaaccc
     4981 tttatgtctt taagtaaaat aaccttcatt tactttcacc ttcctcgttt aagtattcct
     5041 gtataactga tgcaattctt cttcctgctt cctctatagt aatatcttta agctgggctc
     5101 cggctatggt catatgacct ccgccaccta gcttctctaa aatcaattgc acgttaatcc
     5161 tacccaatga tctgccgctt ataattaccc caccatccat ctgacccaat acaaaagagg
     5221 cttcaattcc ccgtatggtc aggaggctgt cagccgcttg agcggcaata agggaggggt
     5281 tttttacccc gggcggacac tgggaagtag ctatgttagg cgctaggatc ctcgctttct
     5341 tcaccgtctc ggcccttgcg atataagtct ctatatcgtc tttatagagt tgtcgcgccg
     5401 atgtagggtc tgcccccgac cgtttaagat aggacgctgc ctcaaatgta cgcactcctg
     5461 tcttgaaggt aaagctctta gtatccatgg taatccccgc caaaagtgca tcggcctcca
     5521 atggatccag tctgagcttc tcgtcaaaat attgaaccat ttcggagacc agctcactgg
     5581 ctgaggatgc atagggttcc agatacgcta gggccggatt ctcgataacc tcggcgcttc
     5641 gcctgtggtg atctatcacc gctatcctat cggcaatatc cagcagctcc ggtgcctcgg
     5701 taaagctggg ccggtgcgtg tccaccacta tcaacaggga atacccatcg attttagatt
     5761 cagcatccct aggggagata aatagattct cgtattcctt tttcctttta agctcctcca
     5821 tcaagtactc tatggaggcg ttggattcct ttaatactat atagacctct tttcctatat
     5881 ggctggcaca tctatatagc cccagagccg aacctataga atccaaatcc ggaagttcgt
     5941 ggctcataat aaatatatta taggattgct ccatgagttc ccgaagcaca ccggccataa
     6001 ccctggattt tactttggta cgcctctcta ccgccttggt cttaccgcca taaaaatata
     6061 acttggtccc tttctttaca acagcctgat ccccacctct acctagggcc atatctaggg
     6121 ctgtcttggc aaaatcaatg ctctgggatg ggctttgggc ctcaacaccg acacctatgc
     6181 tcaaagtagg gggtacatga ctgcccatat ctatttcacg gatatcgtct aatatcccga
     6241 atctattgcc ttccagcgcc tccagaatat ttacatccat caccatgaga aatttatcgc
     6301 gatcgtattt ttgccaacct gcactatggt tcatggccca tttgcccaac ctgccctcta
     6361 tttctgcgat gagcaacggt ttgttcgcat cctccgtatt ggccagaacc tcgtcataat
     6421 tgtccacata gatatgggct ataaccaatt ggatctccct gtgctctttt tgaaccaaag
     6481 tctgaactgc gggcttaggg ggttcagcct ctgccgtttc ctgatgctca gataccccca
     6541 aaaatccaaa tattcttctt aaccaagacc atcttttcat ggatatccaa cctccatttc
     6601 agtcccccct acggctgtcg tacgccttcc tggcgccaaa tatttgttca aagatcccca
     6661 agaatgtcaa agcgaaggac aagaacacga gggttgctat caatatcagc acacctacaa
     6721 ttcccggtac gcgcctctca cccagaaaaa aggttgctac ggataacccc tggatggtaa
     6781 aaatgaaggt gaatactgcg gaaatggtgt ataataccac atcaaaattg gggatcttaa
     6841 cccagctgcc tatggctgaa acgagcatca gccccaaaaa tccccgtccc gtccccctag
     6901 ggaggttcca cctcttaaac gggggcacct ttggtgtctg ggagccgagt ctttttagga
     6961 tccaccttga tatcagaaag tttaagcccc cgacaaatag tgaggttata agtaacatgg
     7021 ccggaaatag cggaacaacc tctttcatct ggcttaggag aaattctata aacttatccg
     7081 ctggatatgc tttatccact atacccaagg catgataaaa ttcccttatt gccttcaagt
     7141 caacaatacc tgagttcaat aagtccccca tcctcttcca aagaaaatca aaaaaactct
     7201 cacccatcat ccaggaaaag acctgtaggg atagcaggac ggataacaat accccgccgg
     7261 cggacaatat cacagattcg ttcatcccca gatccctttt atacccaatc gtcaagccta
     7321 ggactacgaa caagcttagg cataacatta ttattcccag caggggatca cctaatagcc
     7381 ctatcaaaat tgggggtacc accaggctca agatgccggc gagtgtcccc cgtctaatcc
     7441 ctatgaccac tatcggaacc ggaagtatca gtatcaatat attaaataat ggtacggcat
     7501 ataagaccat ggttaataaa gtgataatta cccccactac tatgccttcc aagattgctt
     7561 ttgcaggttt ctccattata tacactccaa gttaaagttt catgggtttc ccctggattc
     7621 cttcaggtgt cgttgaagct ccgacagatc cccatagctt ttttctatat cgtttccctc
     7681 tattattcca agccgaatct gatcttctat ggtctgatcc aatagcgcat agtggatacc
     7741 cagccttttg cccagtagat agcaagcaac gatgacgtca cctaaatacc cgattaagac
     7801 ctcttgggag gaccttgtgc caccggctaa taggctaaat acctttgcta ttgaatttaa
     7861 catttcactt tttaaccact cgataagccg aatgtttctt gtaatatcaa agtctccagc
     7921 ctttccccgt gccatattat caaaccccat ttccaggcat tttagcgtaa atccatttct
     7981 tctataataa tactattcgc tatcttctca atttttccta cccgttaggg gtaaatcttt
     8041 ggataggatc ccaacatttt gaaaaatctg cattgtttgc gcagccgatt gagggcaatt
     8101 ccaatcttat cctcgcttgc gtgcccttcc aagtccacca agaaaagata ttcccctaaa
     8161 agggtgcgca ttggacggga ttcaattttg gtcagattta tattcaaatc ggcaaatatc
     8221 ttgagagcct gaaataaccc ccccggcttg tggtttacgg taaagaccag ggtggtcttg
     8281 tcctcgccgg tcatctccgc ctcgttagtt gacaggacca caaagcgggt gctgttatag
     8341 ttgttatcct ggatcccctc tttgaggata tctagtccat agatatgagc agcccgataa
     8401 tttgcgacgg ctgcccaagg ttctcggctt tccataacca acatggctgc ggcggcggtg
     8461 ctctctgtaa cctctcgctt gataccggtt gcaagattat ctaaatacct tctgcactgg
     8521 gccaaggcct ggggatggga cagcacctta gtcaaatcgg caattttggt gccgcccttt
     8581 gccatgagac aatgacagat gggaataatt aattctcccg ccacgttaag atccacttca
     8641 tggatcagca tgtctacagt aatattaacg gtgccttcca tggaattttc catggggacc
     8701 acggcttcat ccacctctcc tcccgaaacg gcaaggatca attcctgtat gctagaaaac
     8761 tctatcaatc tatacttaca acccttggtg tagataagag ccgcatcatg ggtgaaggta
     8821 cccacaggcc ccaaataccc tatttgcttc atagttattc tccgttttgg ccttttagtc
     8881 taaaattcat tgtatatgga aggccccgct ttgtcaattt tttttgggtt ccctcataca
     8941 ctgtactaaa cggggagaat gggggtgtat ggccttgaga aaattcaaat ccctccggat
     9001 ggccaagatt ggcatatttg gtgttatcgc tatattattg ggcttggccc ttttgatctt
     9061 tttattcatc gattataata taaatcctgc tatcataacc ttttcccaag ctaaaataca
     9121 agccatagct ataaactccc tcagcgatgc tgtaaaggaa tcccttggta acaatataaa
     9181 atatacggat tttgtcaatg tgctgaccga taaaaatgga aatgtagcca tgctccaggc
     9241 caataccatg aagatgaatg atcttgcggc acaagtctcg gatatggccc aagagaccat
     9301 ccgatccaag ggggacgagg gtattgatat accccttggg actatcaccg gtagcaagct
     9361 cctggccgga atgggcccta aaatacgcgt aaagatcata tccttccccg cggtgtctac
     9421 agattttgat tcagagctcg agagttctgg aataaatcag accaggcaca agatatatct
     9481 taccttacgt gcccgcatta agatcgccat ccccttaaat actgcggata tagatataga
     9541 ttctcgtgta cctgtcactg agaccattat agtgggcgat gtaccgcaga cttacataaa
     9601 tgtagacgat accgataaga tgctaaaatt ggttccgacg gattaagttt tatcaaacac
     9661 aaagcagcag gcgcaaatcc caaacaaatt gccgggataa ggacatatat cccatccttg
     9721 tgaaattatt ggcaatactt tcttttgggg tccatctttg ggatctctaa aacccgcctc
     9781 aatacatcca tcttctccga ccaatttgag ttaacataac ggaatcgata atatattcat
     9841 taggttctaa attgggctgt tggccaatga tatattctta tttcgaaaat aaaaaaagcg
     9901 aagtccctta tccgggcatt ttcgcttttt gttatctcca cgattattcg gttgcgtagg
     9961 gcaataacgc tataactcta gaccttttga tggctgcagt caacttgcgt tgatgctttg
    10021 cacagttccc ggaaattctt ctcggcaaga tcttcccgcg atctgttata tacttacgaa
    10081 gtttataggc atctttatag tcaattgtat ctactttatc aatacagaaa ctgcaggctt
    10141 ttcttctgga tcttctttgc ccgccgcccc tgggtcttcg tctttcttcg gccatacctt
    10201 tctacctcct ccttatcaaa atggaagctc gtcatccccg tcattctcta agggttgaaa
    10261 tcctggggtg acaccaaaac tatcgtcatg tgctctattg acatcgggat ccccgcctgt
    10321 ccttgcctct ccccattcca ggaatcgaac atcgtctgct actacatcag ttgtatatcg
    10381 gcgttgtcct tcaggtgtat catagctccc agtttgaatt cttccggaga ctgccaccaa
    10441 tctgccttta cttaaatact ttgcactgga ttcagcttgg cctctccata ctacaatcgg
    10501 aataaagtcg gcctctcgct cgccctgttg attggtaaag tttcgatcca cggctaatgt
    10561 aaacctggtc acagcaattc cactagaagt gtagcgaagt tccgggtccc tcgttagacg
    10621 tccaattaat acaaccttat ttaacatatt cgacaccatc cttattattc atcgtcacgt
    10681 ataataatat aacgaatcac cccatcggtg attttataat tacgttccaa ttcactaggg
    10741 agctgggatt ccgcgttgaa gttcatcaat acgtagtatc cttcgcgaaa atcgtctatt
    10801 ggatatgcca accttctctt tccccactca tctatctttt cgatttcccc accttcagca
    10861 gtaataaggt tggaaaagcg ctcaaccatg gcttttacgg cctcttcctc cattgtggga
    10921 cgaaggatgt aaattgactc atatttattc atgctagttc acctcctttt ggactttggc
    10981 cctatgtaaa cacatagagc aaggatgtta catcaataaa ttatatcata atcataacgg
    11041 ttgtacaaga aaaattattt tccttttcaa aggatcttaa tcagcattgt ttttgcctca
    11101 ttaaactcga tgctaatctt gttatccttt tccaaccttt tcattatatc gctgaacttt
    11161 ttaaacccaa tctgtctttc atcgaaatca ggatagtcat tttgtatctc cgccttgatt
    11221 atgctgggat ttatcctttt gaaaccgttc tgtttaaatt catccaactt ttcctgaaag
    11281 atagcggccc aatttacccg gtttatctcc cccgttctaa tggggttctc tatctttaac
    11341 ttcactttga gagaattata ttccccaaat aaccggataa ccccatactt atattcaagg
    11401 ttgtttaaaa gcctgccgaa ggaattatat ccataattct tttcactaaa atcgggtttt
    11461 agccggagca atgtcttttt aagttcactc acaaacattg taccgtccgc atcagcctga
    11521 tcgtctatga tcttctttac gacctccacc aattcttttt gggtgcctac agcatcgtcc
    11581 tctttttgca gattacctct ctttcctgta gtaactgtct ctaaaaaaat aaattcgtta
    11641 caggccttaa tgaaaatatt ggaggccacc tcggatcttg agataccaag aacgaattta
    11701 cccatggatt taagttttcc caccaatgga gtataatcgc tgtcccctga aactatacaa
    11761 aaacaatcaa ttaggggatt tgtataggct acttccaagg catctatgac caattgaatg
    11821 tctaagttat tcttttgatt taccccatat cttatagaag gcaatacatt aaaggtattc
    11881 gacagcaacg tttccttaag cccggaaaaa cattcctgat acccatacac cttgcctata
    11941 agtatgtccc cgcgaatctt caccttctca ataacttttt tgagggtatt gacattccct
    12001 cccgtattct ccaaatcgat gaatacggca taattctttt tatccattat aactccgcct
    12061 tacctttgtc ttttgtttgg aattacctta atcttactat ctttctgaag aatttgcaat
    12121 tagtatttgc tgatacacgg ttatatctta taattttcga atggatccga catgctagtt
    12181 ttcagatagg gaaaatccgc ctccttttgt tgtcatggga aacggcatca tcgttccgat
    12241 aatagcggtt ctttttggca cggagttatg atcttatcat gggacttact tcaatccacg
    12301 ctcccactgc ggggagcgac ttacattgcc ggatatttac ctagcagcat ctggcacttc
    12361 aatccacgct cccactatgg ggagcgacct taaaagatat gttaatatag ctatgtaatc
    12421 gacttcaatc cacgctccca ctgcggggaa tgacatctac ataactccgt gcgttatttt
    12481 acctcaatta tacatcactt tatattaaag aacaagtgtt cccttgggtt aattcaggcc
    12541 attgtcgatt tatctcacca tcaattcacg ggtgctctgg gcaaatctat aaaatcagtg
    12601 tggcttacaa atatatttaa aataggagaa aaccattact gtaaaaagca atggttttct
    12661 gtttatgtct gcctatgctg ttttgcccac tgccttgcgc ctgcagggtt taaacacctt
    12721 tcccgtcatc taatattctt cattgaatta atatttctac attgtccctg ccttttcctt
    12781 cataatcttc taaatattta ttattctttc gactaattat ctcaatcgat taaattgtcg
    12841 attccggctc ccggcggtac tattggatac accttgtcat cgggatgtat atcgcactct
    12901 attaataccg gctcgttcag ttccaaggca tttttgagca cttcttccac ctgctggttt
    12961 ttatctatcc taaaggcctt aatgttataa gcctccgcca attttacgaa gtccacatcc
    13021 ggtccaaaag aagtatggga atacctttta ttaaagaata gatcctgcca ttgcctcacc
    13081 attcctaagg agtgattgtt tagtaccaat tgtatgacgg gaatcctgta cctggagatt
    13141 gtagcaagtt ccgttgagtt catcttaaaa ctcccgtctc ccgcaatatt cactaccctt
    13201 tccccgggcc tgcctagagc tgccccgatg gatgctccaa gcccatatcc catagtgccc
    13261 aatccgcccg aagatataaa ggagcgggct ctggtaaact tatagaattg ggctgcccac
    13321 atctgattct gacccacctc tgtggatata atagccttcc catccgtcag atcgtacagt
    13381 ttttctataa tatattgggg actaagtgat ccgttcatct tatatgtcaa gggatgctcc
    13441 cgcttccatt ctgttatctg aagattccag ctgttctctt cctttatctt caccttttca
    13501 ttgaaaaagt caagaacctg gcccacgtcc ccgcatatct gaatatgggt agatacgttt
    13561 ttacccaatt ccgccggatc tatatccaca tgtaaaatcc tcgcctttgg ggcgaatttt
    13621 tccacatcgc ttaccaccct gtcactgaac ctggcaccga cggcgattaa aagatcgcaa
    13681 ttggtaaagg catagttaga atatctggtc ccatgcatcc ccaccattcc catataatat
    13741 gggtgggttc cgggaaaagc ccccatgccc atgagggaac agcttaccgg tgcctttatc
    13801 ctctcggcga attccacaag cctctcgttg gcacctgcaa tgtttatgcc tccccccgca
    13861 tagatgaccg gtctctggca atcgtttatg gcttctatgg ccctgtatac gttttcgtcg
    13921 agaccgtctt cccttgttcc atccccacag tctttattgg ttggggccgg tatatagtcc
    13981 accattgcta cttgaatatc cttaggtata tctactagca ccggacccgg ccgtcccgac
    14041 cttgcgatat ggaatgctcg cctaattata tgcgcaagct gctctggatc cttgacgata
    14101 tagttatgct tggttatggg gtgagtaatt cccgtaatgt ccacctcctg aaaggaatcc
    14161 ctcccaatca atgagctggc aacctggcct gtaaacacca ccatgggcac cgaatccata
    14221 tatgccgttg cgatgcccgt aacggtattg gttgccccgg gtccggaggt tacaaccact
    14281 acccccacct ttccggttgc cctggcatat ccgtcagctg cgtgggatgc cccctgttca
    14341 tgagcagtca atatgtgtct gatatcctcc acgtcataaa gggcatcata tatgggtaaa
    14401 actgcacctc cgggataccc gaaaatagta tctacaccct gctccctcaa acattcaatt
    14461 aaaacctgcg cgccttgcaa tttcatactt ctgcctccct ttctggttct aaataagcat
    14521 tgggaccgcc caatccttcg aataagacaa aaaaaccttc catcccgctc tgggacgaaa
    14581 ggttatattc gtggtaccac ccatttttgc tgttatttta caataatagc ctcagtagga
    14641 tacagccata aggcttatat ccctgggctg ttacgggccc attcccgatc aatcctacta
    14701 aggatttcag attgacagct ccgggatgat cttcgaaaca gctaccgggt atcggctttc
    14761 acctatcgcc gactctctgg gacctgggta aacaatttct actattccct tcatggcctt
    14821 tcccgtataa tacacaattt ttgataatta aaaattattg taatataaat tcaatgaaaa
    14881 ggcaaggtat attacaaata tttttagaaa aaccgggttg taacgttaaa tgccgaggga
    14941 ttcccaaagt atcccatacc ggggtttcag gaaggcccta tcccgacaat gcctttacct
    15001 gactaactcg gcgatggcat gggcatatat cctagtattt aagaccagat cctctatatc
    15061 tatatattca tccttttgat gtgccagctc gggtcttccg ggaaatacgg caccaaaggc
    15121 tacaccggta tcaagggctc tggcataggt cccgcctcca atggataaag gctccgccga
    15181 ctcttccccg gtctgctcct tatagaccct gattagggtc tccaccaaaa agtgatcctt
    15241 gggtacgtgc aggggttttt gtccatgcaa gacccttacg tcgatcccag tccccttaag
    15301 tttttcgtgg accttcaata aaatttcccc ttgggtatac tcgacaggga aacggatatc
    15361 caacacagcc tttcctacct cgctatctac atggattacg cccaaattta gggtaagatc
    15421 cccggataca tcgtcacggc atttaatccc taatccttcg ccagtggtat ccatacctac
    15481 aagtttataa aggtctgcta taaatttatc aataggttca ttcccaaggc ccctatccca
    15541 tagatccttt agcgccttgg cgattgcatt ttctcccttt gcaggagtgc tgccatgggc
    15601 tgatactccc ttgacgttta tggggtccgc ctttccttgg agcatatagg tgcagttgtc
    15661 ggggaccatg tttgcccttg atccgccttt taccctaagg atgggggatc tttccccatt
    15721 gcctgcccca ttaaattctt tgcataattc aatatggact attccttttt ctgcatgaat
    15781 cacgggatag ttagcatccg gggttatacc aaagtccggc atggactgct tattgaagta
    15841 gtagtccata tcggcccatc cgctctcttc atcggtaccg agaatgaggc gcaccttctt
    15901 attaaccgat actccagatt ccatcacggc cttcatagcg taaagggctg caagggcagg
    15961 ccccttatta tctatggctc ccctgccata gatcctattg tcatggatct caccgccgta
    16021 ggggggatag gtccatcctt caccttcggg aacaacatct aagtgggcca atataccaag
    16081 ggtttcgctt ccctgaccaa attcaataaa gcctgcatac ccatccacat tccccaccaa
    16141 aaaacccaat ttctcaccta attccagagt atactccaaa gccctattga tcccttcgcc
    16201 aaaaggctta ccctctaacg gatccccctt tacgctgggg attgcgacca gctcctgggt
    16261 agattttatg agtttacccc tgtaaaaatc cactattttg tccaatggca tagctttatc
    16321 ctccaatcat agactatgtg taataaaacg aagactagaa taaatctccc aaatcaaaac
    16381 ccttgccctc tggagccttg ggggattcta gaatatacaa caatccatag gctgtggtac
    16441 aattgagggc taaaataagc accaaaacca ccatttccca tacctttgcc tattatacca
    16501 taggtttacc cttttaaaaa ccggcataag ggtttataat ggatgtgagg ggaaaaacac
    16561 catatataag agtatgatat cacccatcca tagaataagt cgaactgaat tcacatgaaa
    16621 tattcagctt tggaaatgaa tctttccaag acacctttct cctttaggcc cagctgcagt
    16681 ctcatccggg gtgataccaa tatgtagcgc tgatcatgct atagaatccc aaatttataa
    16741 ggaggtctac aattgaaaat tataatttca gagagcaggg aagaatttaa tgggacggct
    16801 gccgatattg ttcgccgcca aatccaggac aaacccgaca gtgtccttgg tttggcaacg
    16861 ggcagtaccc ccctcgggct atataaccta ttggtatgca tgggcataaa gggtgatttg
    16921 gatttttcaa ggataaagac ctttaatttg gacgagtata aaggcttatc aagggaccat
    16981 ccccaaagct attatcatta catgtatgag aaccttttta gtaaaattaa cataagggac
    17041 gaaaatgtca atatcctcaa tggcgagaaa gccgattcat atagggaatg cgccgaatat
    17101 gaaaagagga tcaaggcctc cggaggcatt gatctacagg ttttagggat cggacataat
    17161 ggacatatag gtttcaatga acctggaacc cccttcgatt ccatcaccga tttaatcgat
    17221 ctaacggaca gaaccattga ggccaattcc agattctttg attcccccca tcaggtaccg
    17281 cgccaagcgc tatccatggg aatcaagaca ataatgcaag cgcgcaatat cattcttttg
    17341 attaccggga gcgccaaagc aaggattgcg gctaaggcat tgaggggctc cattacaccg
    17401 gatgttcccg cttccgtcct gcaactacat ccctttgtta cggttatctt ggacaaagaa
    17461 tccgcagcat acttatgatg atacgggggg ccattccctg tatctatagg caactaatct
    17521 ctaaaattaa aaacccttac agaacccatc cacctaatcc ctaaatccca tcttcttcgg
    17581 ttgctatatg attttacttc agatatatat aaaatataga gataaataag tagagccacc
    17641 atctcatagg tcttgcctat aaaatggtgg ctcttgattt ttgtataagt taggctgtgc
    17701 ccatgctatt tatctacgac ctttgaaaac ataaagtact tatatccgag gggtgaagaa
    17761 tagaatcctt caagcttcgg gctctttagg tatatatcaa catagtaata tataggtgcc
    17821 aacatggatt cttcaaatat gatatcctcg gcttggtgca tgagtttcat gcgctcttcc
    17881 ctgtccgaag aagcctttac ctgggaaatt agggagtcat attccttatt ggaccactgg
    17941 gcatcgttgt ttccgccgcc ggttatccac atatccaaga aggaaatagg atcgttgtag
    18001 tcacccaacc aaccatttcg ggcgatgtag tattcgccgt tctttctggt attgaggaag
    18061 gtattccatt cctgggaagc gagttcgatg ttaaccccga tctctttcca catatcctgc
    18121 agggcttcac caatctgctg atgccctgtg ctctcgttat agagatactc tatggtcgga
    18181 attccctctc cgttggggaa tccagcctca gccaagagtt gttttgcctc ttccaggttc
    18241 ttctcataat ctacgggatc atagtaatcc ccgcctacct cacggaattc tttggtggga
    18301 tcggcgtcgg taagcccggt tgctacaaaa gcgcctgccg gttgctggcc ggcctgaccg
    18361 atcttctcac agataaattc cctgtcgata gccaaggtaa gagctttacg aactttgggg
    18421 ttatccagcg gttcctttgt aacgttaaag cttatataat acgtacccag ctggccttgt
    18481 atgaagaatt catctttgtc cttccaggcc tcaatctcat cgttgggcat ggaatcggcc
    18541 aggagaatct cgccattttc aaaagcggcc aaaatagccc cgtcgtcgtc cataagcaag
    18601 aatttgatgg cttcggggcc tatctcactc ttgttccaat aattagggtt cttcttgtag
    18661 gtaatgtgag attcgtgtac ccattcgtcc atgacatagg gaccatttcc tatgtaggtt
    18721 tcaggttttg tagcccatcc gtcaggatca gcttccacta tatccttgcg aacggggaag
    18781 aatgtgggga atgcacatag ttccaagaag taaggggcag cggtaataag ctttacttcc
    18841 agagtctttt catcaagtgc cttaacgttg aggtcgtgtg caggcaccca atcctcatca
    18901 tcttcatcga catcctggcc tgcgtcataa ccatcgatga cctcaaacat atactcatag
    18961 tcagcagccg tttcaggatc aacagccctc ttccaagagt atacataatc ctcagctgtc
    19021 accggctcgc catcgctcca ttttaagccg tcacggaggg taaatgtata ggttaggcca
    19081 tcttcgctga cctcatagga ttttgcctgc ccttctacag ggaccccatc cttatcaaga
    19141 ttcatgaggc cttcaaatgc gtggattagc agtgtagccc cgtcaacggc tgagtttaat
    19201 gcaggatcta tggtgtcagg ttctggtcct acattgacaa ccaattcttt ggcgccggaa
    19261 gcaccgcagc cggctgcgag catcccaaaa gtcattataa tgactaaaag taatgcaatt
    19321 cttttcatac cttgtttctc tcctttttat atattttatc tattgaatat agtggttcca
    19381 acagataaat ttataattat caaggggcgt caggcacagc ctaaaagccg ccccttaatt
    19441 tactctttaa tagccgccgc ctattttacc ctgtccaggt ggtggcaggc agcaaaatgg
    19501 cccttggaaa cctccctaaa ttccggttcc tccctggcac ataattcgtc ggcataggga
    19561 caacgggtac ggaatcgaca accgctcggc ggatccaatg ggctcggtac atcgccttct
    19621 aatactatcc tttgcctttg tcggctggca tccggatcgg gcacgggtat agcagaaata
    19681 agggatctgg tatatgggtg gacgctatga aacgatagct cgtagctatc ggccagctca
    19741 accaatttac cgagatacat aacgccaata cgatcgctta tatgcttaac taccgacagg
    19801 tcatgggcta taaacagata agtcagtccc atatcctcct gaagctcttc aaacatattt
    19861 acaatctggg cttggatgga tacatcaagg gcagaaaccg gctcatcaca aactatgaat
    19921 tccggatcta cagccagggc ccttgcaatg cctatcctct gtcgctgccc tcccgagaat
    19981 tcatgggggt agcggttagc atgttcgctg ttaagaccta ccctgtccag catttctata
    20041 attctgtcgg ttctctcctt tttagaatgg gccagcttat gaatatctat gggctcaccc
    20101 actatatctc cgacggtcat tctgggattt aaggaagcat aggggtcttg aaatacgatc
    20161 tgcatctttc ttcggtaagg catcatatcg gtatgagtga tatctacacc gtcataaata
    20221 atccttccat cagtcggctc ataaaggcga agcaaggaac gccccgtcgt tgttttgccg
    20281 catcccgatt ccccaaccaa tcccaatgtc tcaccctttt ttatataaaa ggatacatca
    20341 tctacagcct taacgtattt ggtgcgaaaa aggccgtcac ttatgggaaa gtattgcttt
    20401 aaattcttta cctcgataag cggtggttcg cttgccttta tatctaatcg tccctcatcc
    20461 aaatgttttt ccattaccct tcacctccag cggcggcctt aaaggcttcc ttctgcaaaa
    20521 gccagcaggc gcaatcatgg gtatcgctga ttttggtata ctccggcatc tccctgaggc
    20581 atatcttcat gcatttatcg cagcgaggtg caaatggaca tcccgccggc gggttaagca
    20641 tatccaccgg tgtcccttca atgggtatca gtttttcatg gctctttgta tttattttcg
    20701 ggatactgcg aagtagcccc ttggtatact catgctgggg attataaaag atttcatcgc
    20761 tggtcccgga ttctacgatc tttccgccgt acataactac gatcttctgg cacatgtttg
    20821 caactactcc cagatcatgg gtgattataa tgatggacat attgatcttt tccttaagct
    20881 ccatcatcag ctccattatc tgggcctgta tagttacatc caaagctgtg gttggttcgt
    20941 cggcaatcaa tagcttgggc tcacaggcca gggcaatagc tatcatcacc ctttggcgca
    21001 ttccccccga cagctcatgg ggatattgct ttaacctctt ttcgggttcg ttgataccga
    21061 caagggtcaa tagatccaat gacctcttat gggcctgttc gttgtccata tccgtatgaa
    21121 ggagtattac ctccctaatc tgattgccta tagtatacac gggattaagg gatgtcatag
    21181 gatcttggaa tatgatgctt acctcattcc ccctaatctc ccgcatttct ttttcagtca
    21241 tatcgttaat tctatgccca ttaaaatcta tagtgcctcc gataattctc cccggaaagg
    21301 gaacgaggcc cattatagaa taggctgtca cagatttacc gcttccactc tcacctacga
    21361 tacctagaac atcaccctca ttcatcttaa aggtaacatc gtttagggct ttaacctccc
    21421 cggcaggcgt aaagaatgaa agtctgagat tttttatatc caataacaca ttactcataa
    21481 tttttcctcc ctagttcttc aacctcggat cgaaggcatc cctgagacca tccccaaaga
    21541 gattaaagga caatatgatg aggctgatgg cgacggcagg ggctaagagc cgatgaggat
    21601 atgatcttat accgtccact gcctgggcgg ctaaagatcc aagactgggc attggtgcgt
    21661 ttaccccaag acctaaaaag ctaagaaagc tctcggtaaa tatagacgag ggtatctgca
    21721 aagtggtggt tacggtaagg gtgcccacac aattggtcag caggtgcttc tttattatcc
    21781 tgctatggga ggcacctagg gctctggccg cggtaacaaa ttcctgctcc tttagcgtca
    21841 aaatctgacc cctgacgatt cgtgccatgc ccacccaata caataatgaa aagacaataa
    21901 atatacttat aagtccggtt cctactatat taatc
//
LOCUS       DASRNX010000115         6152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_22760,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000115 DASRNX010000000
VERSION     DASRNX010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22760"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..359)
                     /locus_tag="VFD89_04630"
     CDS             complement(66..359)
                     /locus_tag="VFD89_04630"
                     /inference="COORDINATES: protein motif:HMM:NF018633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HZJ57508.1"
                     /translation="MNEGNNLVELIDDDGNTLSFEYLMTIDFGDNEYVVLTANEPPKD
                     PSQEFNGGEVVILRVENDGDQDSVYVSIDDEDELNEVFDAFNQIMAQSEDEGP"
     gene            complement(352..795)
                     /gene="ruvX"
                     /locus_tag="VFD89_04635"
     CDS             complement(352..795)
                     /gene="ruvX"
                     /locus_tag="VFD89_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZJ57509.1"
                     /translation="MRILAMDVGDRRIGMAVSDSLGWTAQGIETLIRKGEKYDLEKIN
                     GILDLYKPDKMVLGLPINMNGTVGPQGEKVKRFGELLREQAFSGDVIYWDERLTSVQA
                     NRIMIDADVSRKRRKENVDMMAAHFILQSYLDYMNNQKKGGNADE"
     gene            complement(862..1809)
                     /locus_tag="VFD89_04640"
     CDS             complement(862..1809)
                     /locus_tag="VFD89_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZJ57510.1"
                     /translation="MIYNTLGQTGIRVSRLCFGSLTLGPLQKNLSLEDGAAVIKRAAG
                     LGVNFIDTADLYGTYPYIKEALRDFPNLVISSKSYAYDSKTAEETLNRALKGIGRDYI
                     DCFLLHEQEGPLTLKGHWEAIEYLSRCKERGLIRGIGISTHHVAGVRAAAITPEIDII
                     HPIINYRGIGIVDGNLNDMEQAIALAWANGKGIYAMKSLGGGHLIPNFQKAFDYILNF
                     PYTHSVAVGMQRCEEVEINVDIFNGTTPAKAKLDSIRDYERSLLIQDWCTGCGRCIRR
                     CQQQALSIGENDKSQVDIEKCILCGYCGSVCMELAIKVI"
     gene            complement(1830..2084)
                     /locus_tag="VFD89_04645"
     CDS             complement(1830..2084)
                     /locus_tag="VFD89_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HZJ57511.1"
                     /translation="MKFSLEKEYDKTPKEILLRVHSALKEKGYDPINQIVGYLISGDP
                     TYITSHQNARALIRRLERDELLEELIIHYIDSNEKNRCNT"
     gene            complement(2411..5056)
                     /gene="alaS"
                     /locus_tag="VFD89_04650"
     CDS             complement(2411..5056)
                     /gene="alaS"
                     /locus_tag="VFD89_04650"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZJ57512.1"
                     /translation="MEKMGLNQLRDSFLKFFEGKNHLILPSASLVPKDDKSLLLTNSG
                     MAPLKAYFTGQETPPSPRVTTCQKCIRTPDIEIVGKTARHGTFFEMLGNFSFGDYFKR
                     EAIIWAWEYIIEVLKLPLDRLWITIYFEDDEAHKIWTEVVGVDPSRVVRMGKEDNFWE
                     IGVGPCGPSSEIFFDRGREHGCGSEDCAIGCECDRFVEFWNLVFTQFDRDEEGEYHPL
                     DHPSIDTGMGLERIAAIMQDVNSLFEVDTIKNIMGAVSRISGVEYGKNDNTDISLRVI
                     TDHIRSTVFMISDGIIPSNEGRGYVLRRILRRAARHGKLLGIRDMFLSDLAKVTIKES
                     GDAYPEIGEKQKYILKVIQVEEERFNETIDQGLVILRGYIDAVKKEGAKALDGESAFR
                     LYDTYGFPLDLTKEILEEEGLGVDEAAFDKEMKAQRERARAARHETNYMGGDDPTYGL
                     LTPEDITDFVGYDTLNARSRVIGILKDNTRVESAYAGDHVLIILDKTPFYAESGGQVS
                     DSGILHNDMTKVEIQDVSNPHEKHIVHIGKVLAGGISVNQELNARVHAGDRISTARNH
                     SVTHLLHKALRDVLGVHVEQAGSLVAPTRLRFDFSHFAPLTNGEIDRVEEIVNQKIME
                     GMPIDIIETTYDGARDMGAMALFGEKYGDMVRVVKMGDYSVELCGGTHLSNTGQAGLF
                     KIISEAGIAAGVRRIEAVTGIGAREWIKQLDGTVNRISETLKTNPKGVIQRAEGIMEQ
                     VKEYEREIQLLKRQLAQGTIDGLISNKKTLNGIQFIAANVEQQDMNGLREIADTLRNR
                     LKSGVVVLASEHQGRVNLVAAATKDMVGKGIHSGNLIKEVAKITGGGGGGRPDMAQAG
                     GKDPLKIAQALNEVEILLAQQLGIQ"
     misc_binding    complement(5100..5309)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5528..>6152)
                     /locus_tag="VFD89_04655"
     CDS             complement(5528..>6152)
                     /locus_tag="VFD89_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZJ57513.1"
                     /translation="SYIRGRMADAADGAGKIATIFTIPILSFYFIKDREYFKKITLRL
                     IPIKIRGPTHRMAGEISKILNRFIRGQILVASIVGILATIGYLIIGLPYAAVMGLFAG
                     IFEIVPYLGPVLGAMPAGIIAILHSPSKLIASIMVMIIVQQLESNIFTPKIMGAHVGM
                     HPVYIILALWIAGTSFGIIGMFFAVPAVLILRVFIKHVYLGIVSGDC"
BASE COUNT         1580 a         1625 c         1060 g         1887 t
ORIGIN      
        1 tagtctaatt atacactaaa acggaattac tttccatagt acttttttca taaaaaaaga
       61 cccctctagg ggccttcgtc ttcgctttgt gccattattt gattaaaggc atcaaaaacc
      121 tcatttaatt cgtcttcatc gtctatgcta acatatacac tatcttgatc tccatcgttt
      181 tccaccctca aaataaccac ctcgcccccg ttaaattctt gggacggatc cttggggggc
      241 tcatttgctg tcagtaccac atattcattg tctccaaaat caattgtcat taagtattca
      301 aatgataagg tgttgccatc atcatctatt aactctacca gattatttcc ttcattcatc
      361 ggcgtttccc cccttttttt ggttattcat ataatccaaa tagctctgca gaataaaatg
      421 ggctgccatc atatccacat tttcttttct tcttttcctg cttacgtcag catctatcat
      481 gatacggtta gcctgcactg aggtaagcct ttcatcccaa tagataacat cccctgaaaa
      541 agcttgttcc cttaacaact ccccaaatct tttcactttt tccccctggg gacctacggt
      601 tccgttcata ttgataggca agcccaaaac cattttatcc ggcttataga ggtccaatat
      661 cccattgatt ttttccaaat cgtatttttc gcccttcctt atcagcgttt caataccctg
      721 ggccgtccaa cccagactat cactaacagc catgcctata cgtctatcgc caacatccat
      781 agccaaaatc ctcaaattgt ggttgccctc ctatataatc ttagttatat ccgattccat
      841 tactcctaaa acagatctca cctaaataac cttaatagcc aattccatgc agacagaacc
      901 gcaatagcca caaagtatac atttttcgat atccacttga gatttgtcat tttcacctat
      961 actgagcgcc tgttgctgac atcgccttat gcatctgccg cacccggtac accaatcttg
     1021 tatcaggagg cttctctcgt agtctctaat agaatctaat tttgccttgg cgggagttgt
     1081 cccattaaat atatccacat taatttcaac ctcttcgcac ctttgcatgc ctacggctac
     1141 agaatgggtg taggggaaat tcaaaatata gtcaaaggcc ttttgaaaat ttggaataag
     1201 gtgtccaccg cccaaggatt tcatagcata tatgcccttt ccgttggccc aagccagggc
     1261 tatagcctgt tccatatcat ttaggttccc atccactata cctatacccc gatagtttat
     1321 aatgggatgg ataatatcta tttctggggt tatggcggct gccctaaccc cagccacatg
     1381 atgggttgaa attcctatac ccctaatcaa tcctctttcc ttacatcggg ataaatattc
     1441 aatggcctcc cagtgtcctt ttaaggttaa cggaccctcc tgctcatgca ataaaaagca
     1501 atcaatataa tccctgccta tacccttcag agccctattt aaggtctctt cggctgtctt
     1561 agaatcataa gcatatgact tgctggaaat caccaagttt gggaagtccc ttaacgcctc
     1621 ttttatatat ggataggtcc catataggtc agctgtatcg ataaagttaa ctcctagtcc
     1681 tgctgccctc ttgattactg ctgcgccatc ttccagggaa agattctttt gtagaggtcc
     1741 taaggtcaat gagccaaaac aaagtctgga gacccttatc cccgtttgcc ctaaagtgtt
     1801 gtaaatcata ttctaacaat agccttcttt catgtattgc acctattttt ctcattggaa
     1861 tcgatgtagt gaataattaa ttcctctagt aattcatccc tttccagccg tcgaattaaa
     1921 gccctagcgt tttgatggct agtgatatat gtgggatccc cggatattag atacccgact
     1981 atctggttga ttgggtcata tcccttttcc tttagggcac tatgcaccct cagcaaaatt
     2041 tcttttggtg tcttatcgta ttccttctct aagctaaact tcatagtctc ctgtaaatcc
     2101 ctatccatag atcgtaccac ctcctttata cttaaaatgg gccctgcccc cctatttgtc
     2161 ccgccttaat tgtattccct ttgctgcatg cttaggataa tcatgcagca ttaatgtccg
     2221 gatgctgtaa gatattgccc tacatttata tgttacgaag agtacattat ttttacacat
     2281 ggtaaggcaa tatagccgcc tttgcgctga agggtccttc ccgttacata tacagtataa
     2341 ctacagtcgg tccatatcac aagcattgat tttgtcatac ttaagggcta tccatccggt
     2401 gtgatgatgt ttactggatc cccaactgtt gtgccaataa aatttccacc tcattcaacg
     2461 cttgggcaat ctttagcggg tccttgccac ctgcttgagc catatcgggt cgtccgccgc
     2521 cgccgccccc tgtgatttta gctacctctt taataagatt gcctgaatgg attcctttac
     2581 ccaccatatc cttggtggcg gcagcaacca ggttaaccct gccttgatgc tcagaggcca
     2641 gcacaaccac cccggacttt aacctgtttc ttagggtatc agctatctcc cgcagcccat
     2701 tcatatcctg ttgttccaca ttggccgcta taaactggat cccatttaat gttttcttgt
     2761 tggatataag cccatctata gtaccctgcg ccaactgcct ttttaagagc tgaatttcgc
     2821 gttcatattc cttaacctgc tccataattc cctctgctct ttgtataacc cctttggggt
     2881 ttgtttttag ggtttctgag atccggttga ccgtcccgtc caattgtttt atccattccc
     2941 tggcacctat tccggttacc gcctctatcc tcctcacacc ggcagctatt cctgcttcgc
     3001 ttataatttt gaagaggccc gcctgccctg tattggaaag atgggtgccc ccgcatagtt
     3061 ccacgctata atcccccatc tttacaaccc ttaccatgtc accatatttt tccccaaata
     3121 gtgccatagc ccccatatcc ctagcaccgt cataggtggt ctctatgata tcgatgggca
     3181 tgccttccat tatcttttga ttcacgattt cctctaccct gtcaatttcc ccattggtta
     3241 agggagcaaa atgtgaaaag tcaaacctca atctagtagg ggcaactagg gagcccgcct
     3301 gctcaacgtg gactcctagg acatccctta gggccttatg caacaggtgg gtgacagagt
     3361 gattgcgtgc cgttgagatt ctgtcacctg catgaactct tgcattcaat tcttggttaa
     3421 cgctgatacc ccctgcaaga accttcccta tatggacaat atgcttctca tggggattgc
     3481 taacatcctg gatctccacc ttggtcatat cgttatgcaa tattccgcta tcgctgacct
     3541 gtccaccgct ttctgcatag aagggggtct tatctaaaat tatcagtaca tgatcacctg
     3601 cataggccga ttcaacccgc gtattatcct ttagtatgcc gataaccctg cttcgggcat
     3661 ttaaagtatc atatccgaca aagtctgtta tatcctccgg tgtcaaaagc ccataagtcg
     3721 gatcgtcccc gcccatatag ttcgtctcat gtcttgcagc cctagccctc tccctctggg
     3781 ctttcatttc cttatcgaat gccgcttcat ctacgccaag accctcttcc tccaatattt
     3841 ccttggttaa atccagggga aaaccatagg tgtcataaag tctaaaggca ctttcaccat
     3901 ctaatgcctt tgcaccttcc ttcttaacag catctatata gccccttaga ataaccaatc
     3961 cctggtcaat ggtctcgtta aaccgttctt cctcaacttg gataaccttt agaatgtatt
     4021 tctgcttttc ccctatctcc ggatatgcat cccccgactc cttgatggtt accttcgcca
     4081 gatcagataa aaacatatcc ctaattccca gtagttttcc atgcctggcg gccctcctta
     4141 gaattcttct aagtacatag cccctcccct cgttggatgg gataattcca tcggagatca
     4201 taaaaaccgt gctccttata tgatcggtga taaccctaag ggatatatcg gtgttatcgt
     4261 tcttgccata ttccaccccg gatatacggc tgactgcccc cataatgttt tttatggtat
     4321 ccacttcaaa gagggaattt acgtcctgca taatcgccgc aattctctcc aatcccattc
     4381 ccgtatcaat gctgggatga tccaatgggt gatattcccc ctcttcgtcc ctgtcaaatt
     4441 gcgtaaatac taagttccaa aattccacga atctatcgca ctcacagcca attgcgcagt
     4501 cctcgcttcc gcaaccatgc tccctacctc tatcaaaaaa tatctctgaa gacggaccgc
     4561 atgggcctac accgatctcc caaaagttat cttccttacc catacgcacg acacgactgg
     4621 ggtctacccc tacaacctct gtccatattt tatgggcttc atcgtcttca aagtatatgg
     4681 taatccaaag cctatctagg gggagcttta ggacctcaat tatatattcc caggcccaaa
     4741 taatagcctc ccttttaaag tagtcaccaa aggagaagtt accgagcatt tcaaaaaaag
     4801 taccatgcct ggctgtttta cctactatct ctatgtccgg ggttctgata cacttctggc
     4861 atgtagtaac ccttggactg ggcggcgttt cctgtcccgt aaaataggcc tttaggggag
     4921 ccatacccga attagtcaat aatagactct tatcgtcttt aggcaccaac gaagcgcttg
     4981 gaaggatcag atgattcttc ccttcaaaaa actttaagaa actatctcgc aactggttta
     5041 aacccatctt ttccattgtt attcctcctg agaatataat aaaaaaagcc ccttctatca
     5101 gaagggacgt aaatatacgc ggtaccaccc tcgttatcga aagctaattc gatcactcag
     5161 tacgtttaac ggcttataac cgccacagcc tataagctta gctgtggtgc tccgggacgg
     5221 cttcaaccaa ccatatccga agggctctca ctctcccctt ctcgcttaag gactgggctt
     5281 gatctactac ttcctttcat agcatttaac ccatatttag agaatttcaa aaacacacca
     5341 tacgatattc aggttcgaaa caccgatggt tggattttta taaatccgga tcgatcaatt
     5401 cttcattaga tcgcagtccg tgctcattat ataaaatgta atgggatctg tcaatattta
     5461 gctttcacag atatatttat aggcatacaa aagcctcgga tgatccctaa aatctttaag
     5521 gctgttttta gcaatcgccg gaaactatac cgaggtatac gtgcttaata aagaccctca
     5581 atatcaatac agccgggaca gcaaaaaaca tgcctattat gccgaaggat gtccctgcta
     5641 tccatagggc cagaattata taaacaggat gcataccaac gtgcgctccc attattttgg
     5701 gtgtaaatat gttactctcc agttgttgaa ctatgatcat aaccattatt gaggctatca
     5761 actttgacgg tgaatgtaat atagcgataa ttccagccgg catcgcaccc aaaaccggac
     5821 ccagatatgg tacaatctcg aatataccgg caaacaggcc catgacagca gcatagggta
     5881 acccgattat caggtaacct atagtggcaa gtatgcctac tatagaagct actagaattt
     5941 gtccacgaat aaacctgttt aatatcttac ttatctctcc ggccattcta tgggttggtc
     6001 cccgaatctt gatcggtatt aaccttagag taattttttt aaaatattct ctatccttta
     6061 taaaatagaa gcttagaatg ggtatggtaa agattgttgc gattttgccc gccccgtctg
     6121 cagcatcagc catccgtccc cttatatagg at
//
LOCUS       DASRNX010000116        10941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_710803,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000116 DASRNX010000000
VERSION     DASRNX010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10941
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710803"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1702)
                     /gene="rlmD"
                     /locus_tag="VFD89_04660"
     CDS             complement(41..1702)
                     /gene="rlmD"
                     /locus_tag="VFD89_04660"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HZJ57514.1"
                     /translation="MNNRKLNDIPVIKNRDYIIDIDNLGSNGEGIGRIDGFTMFVSGA
                     LPGEKVKIKAVKVGKRFGYGKLIDILKSSNQRVERLCQYARQCGGCQLQHMSYNLQLD
                     FKRQLVKDAMERIGNLEDVIVHDTMGMEDPWHYRNKMQFPVGMSRGKLAIGFYAPRSH
                     NIIDMDSCHIQHSINDTIIKVVRDYINEFNIPVYDETTHKGIIRHLVSRVGFDSGEIM
                     VIIVTNGTQLPKKDSLIKALKDNIPGLVSVVQNVNKKKTNVVLGGQNILLWGKDHIVD
                     SIDGLKFRISPLSFFQVNPVQTAILYNKALEYAGLTGREVVIDAYCGIGTIALFMARK
                     AAKVYGIEVVPEAIGDAKINALENGIHNTEFIVGQAELVVQDMIDEGLKVDVMVVDPP
                     RKGCDVKLLDAIIQAQPDRMVYVSCNPATLARDLGYLSERGYRAVEIQPVDMFPQTAH
                     VETVVLLSQQKPSDRIEVDLDMDEMDVTSAETKATYVDIKDYVLKEHGLKVSNLYISQ
                     VKRKCGLEVGENYNLAKSEDAKQPNCPEDKEKAIEDALKHFGMIS"
     gene            complement(1737..3353)
                     /locus_tag="VFD89_04665"
     CDS             complement(1737..3353)
                     /locus_tag="VFD89_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HZJ57515.1"
                     /translation="MKFQFDPLTYGYPSRRSVVYGKKGMVATGNPLAAQVGLEILKKG
                     GNAIDAAIGVATTLTVVEPTDNGIGGDAFALVWTGDKLHGLNSSGPSPKSISLEALRN
                     LGHTEIPEYGFIPVNVPGIPAAWAELSKRFGKLPFEEVLEPAIRYAEEGFILQPTVGS
                     GWQASYRVYDKERKKGKEFEPWFDTFAPGGICPGVGDNVKLKDHGKTLRRIAKTLGED
                     FYRGELAQRIDDFSKEYGGFISKDDLADYYPEWVEPISADYKGYEVYEIPPNGHGITA
                     LMALNILKGMDLGPKDSFNSTHFQIEALKLAFTDVQKYVADPRIMKIKTDELLSEGYA
                     RGRRSLITDKAIMPKAGNPSKGGTVYLCTADGDGNMVSYIQSNYMGFGSGLVVPGTGI
                     ALHNRGANFNMDEESDNCVGSCKRPYHTIIPGFLTKGGKPVGPFGIMGGFMQPQAHLQ
                     VMVNTIDYMMNPQEALDRPRWQWVGNKTVEVEHGFDNRLALELMRAGHNIVVRANSGT
                     FGRGEIIWRNDEGVLCGATEPRTDGQVAVW"
     gene            3628..4356
                     /locus_tag="VFD89_04670"
     CDS             3628..4356
                     /locus_tag="VFD89_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014662955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HZJ57516.1"
                     /translation="MGTGYLGTKRQILSHAFKTAFPNTIPIFAGFTFLGMAYGIYMNA
                     SGFSFLYPMLMSLTILAGSIEFVTVGLLLGPFDPLGAFILALTINARHLFYGISMLGK
                     YRVSGLKRLYLIFGMCDESFSVNYMADIPEGVDRGWFQFFVTALNHSYWVLGATLGGL
                     LGHIVEFDTKGLEFIMTALFIVLFLNQWMKEKNHHSSIIGLTVSATCLILFGSSGFII
                     PAMVLILLILTVFKNTFTKAGVVS"
     gene            4353..4685
                     /locus_tag="VFD89_04675"
     CDS             4353..4685
                     /locus_tag="VFD89_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter permease"
                     /protein_id="HZJ57517.1"
                     /translation="MTMNVHEHIITIIAVILGTMATRFLPFILFPADKPTPKYIEYLG
                     KVIPAAVLGLLVVYSLKDVEILTGSHGIPELISIAIVVVLHIWKKQMLLSIAAGTVAY
                     MLLVQFIF"
     gene            4821..6152
                     /locus_tag="VFD89_04680"
     CDS             4821..6152
                     /locus_tag="VFD89_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HZJ57518.1"
                     /translation="MNKRNVIIIGAAGRDFHNFNTYYRNNDSYNVVAFTAAQIPDIDN
                     REYPGELAGGLYPEGIPIYSQDRLADLIGELCVDECVFAYSDVSYEDVMGISAIVNAA
                     GADFRLLGPKSTMIKSTKPVISVCSIRTGCGKSQTSRRVIELLMGFGLKVVAIRHPMP
                     YGDLAAQKVQRFATLDDLKRHKCTIEEMEEYEPHIERGNIIYAGVDYEEILKAAENDP
                     DGCDVILWDGGNNDFSFYEPDLSITVLDPHRAGHELSYYPGEVNLRTADVAIINKVDS
                     AEKDAVQQVENNIRDANPKATIIKAESKITVTEPDVIKGKRVLVVEDGPTLTHGGMGI
                     GAGTVAAQRFGAKEAVDPRPFIVGTLKKTFENYKNIGKLLPAMGYGEQQLKDLEETIN
                     NADCDSVIIGTPIDLSRVININKPYTRVSYELSEIDKPDLKAILKDFVDGL"
     gene            6485..8224
                     /gene="aspS"
                     /locus_tag="VFD89_04685"
     CDS             6485..8224
                     /gene="aspS"
                     /locus_tag="VFD89_04685"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014600045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZJ57519.1"
                     /translation="MDKYRTHNCGELRKGNVKERVRLAGWVSTIRDHGGVIFIDLRDH
                     YGITQIVIDDESLIKGIGREYVISVEGVLRGRSEETINPKIATGEVEVKVDHITVLGT
                     SVSQLPFEIGESTMTREDIRLRYRFLDLRNPSVHENIVMRSKITSHLRRKMEDLGFVE
                     IQTPILTSSSPEGARDYLVPSRKHHGKFYALPQAPQIFKQLLMASGFDRYFQIAPCFR
                     DEDSRADRSPGEFYQLDFEMAFATQEDVLAVAEEVLYDTFSKFSEKRVSPAPFKRIPF
                     AESMLKYGTDKPDLRNPLIIQDLTDFFRDVDFAPFKNKPVRGIVAPNCAGMPRSFFER
                     MLAFATGVGMKGLGYISVLPGMELKGPIVKFLSAEKQREMIDLLNLKEDDTLFFICDT
                     PKMVDKLAGFIRTELGERLDIIDRDSFEMCFITDFPMYGLSENTGKIEFTHNPFSMPQ
                     GEMQALVDMDPLDIKAYQYDIVCNGVELSSGAVRNHRPDIMIKAFEIAGYCEEDIKDK
                     FPALFSAFHYGAPPHAGMAPGLDRMVMLLTGEENIREVIAFPMNANAEDLLLGAPGPV
                     TEAQLREIHIKIR"
     gene            8323..8598
                     /gene="gatC"
                     /locus_tag="VFD89_04690"
     CDS             8323..8598
                     /gene="gatC"
                     /locus_tag="VFD89_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZJ57520.1"
                     /translation="MPRIEDYETMTKISLTKEERGIVSGHMNMLLDSFKELGDIDVSG
                     VEPLVSVLDITNDMRDDVAIKTLSREELLSNAPEQYDGYFQVPRTVD"
     gene            8611..9723
                     /locus_tag="VFD89_04695"
     CDS             8611..9723
                     /locus_tag="VFD89_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZJ57521.1"
                     /translation="MGLFIENYNISGADAVALEDSIMEKGRGVTVGSRILKNFVSPFN
                     ATVVDRLLDNGFKIAGRANMDEFGLLKLSRDDDNMPTGAIRAVSDDVAAFSLCNDLFG
                     RYRREATENDCCYIHPTYGTVSRYGLVPLASSMDQIGIVCKDLAQGFGLLSYISGKDP
                     KDGAMFPESTYDYTKPAGEITLGVPKSVIEKADVDTQDAITEFIDGFANTEIDLEHFD
                     LYSRVMYILSSAEISNNLSRYDGIKFGYRASNFSDLEDLYTKTRSEGFGPDAKSAIIM
                     GAMMLSKDQYGAYYERAMKVRRLIKESIKFDRYNVIVLPCNIGDDPYENLSLYALSPL
                     AGLPSVSFSYKGHGIQLIAGVKNEDLLLSTWEVCKA"
     gene            9720..>10941
                     /gene="gatB"
                     /locus_tag="VFD89_04700"
     CDS             9720..>10941
                     /gene="gatB"
                     /locus_tag="VFD89_04700"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZJ57522.1"
                     /translation="MKYETIIGLEVHVELATKSKLFCPCSTEFGSDINENVCPICSGM
                     PGTSPMINKKAVELGIAAGLLTNSAIARRIAFDKKNYFYPDLPSGYQVTQLFSPICKN
                     GWVEIETDAGKRRINLTQIHIEEDAGKLIHDDWTDTTLVDFNRASVPLIEIVTNPDFR
                     SGEEVVAFLEKLRSLLSFAGVSDCKMQEGSMRCDINLSVREKGTEKLGTRTEIKNMNS
                     LKAITAAIAYESRRHIEALETGDEVLVQETRRWDDSIGETFSMRGKEDATDYRFCPDP
                     DIMPVFIGDEWIDSVRDNLPETAQAKFIRLTEELDLSEYDSEIITRSKNLSDIFDETL
                     KHFENPKEVANWVIVELLAIAKGDNKNEDDVRIDCRKFAKLIELVENKTINRNVGKQV
                     LSLVLEDDIDPVQYI"
BASE COUNT         2938 a         2564 c         2491 g         2948 t
ORIGIN      
        1 taataataca aattatattc catccattcc acctccagcc ttagctaatc atcccaaaat
       61 gcttcaatgc atcctcaatc gccttttcct tatcctctgg acaattcggt tgcttcgcat
      121 cctcagactt cgccagatta tagttctctc caacttccaa cccacatttc ctttttacct
      181 gtgaaatata cagattagaa accttcaaac catgctcttt caacacataa tccttaatat
      241 ctacataagt cgccttcgtc tctgcacttg tcacatccat ctcatccatg tccaaatcaa
      301 cctcaattcg atcactcggt ttttgttggg acagaagaac aactgtctcc acatgcgccg
      361 tctgaggaaa catatccacg ggctgaatct ctactgccct ataccctctc tccgataaat
      421 aacctaaatc cctggctagg gttgcaggat tgcaggatac atagaccatt ctatcgggct
      481 gtgcctggat tatggcatcc agtaacttta catcacaacc cttgcgcggg ggatcgacca
      541 ccattacatc gacctttaat ccctcatcga tcatatcctg aaccaccagc tctgcctgcc
      601 ccactataaa ttctgtgtta tggatgccgt tctccagggc atttattttt gcatccccta
      661 tggcttcggg gactacctct attccataaa ccttcgccgc ctttcgggcc ataaaaaggg
      721 cgatggttcc aatgccgcaa tacgcatcta tgactacctc cctgcccgta agacctgcat
      781 actccagagc cttgttgtag agtatagccg tctgtacggg gttcacctga aaaaaggaaa
      841 gaggcgagat cctaaatttt aatccatcga tggaatctac tatatgatcc ttaccccata
      901 gtaatatatt ctgtccgcct aataccacgt tggtcttctt cttgttaacg ttttggacaa
      961 cactgacaag gccggggata ttgtccttga gcgcctttat cagactatct tttttgggga
     1021 gctgggttcc attggttacg ataatgacca taatttcacc gctatcaaat ccgaccctgc
     1081 tgactaagtg gcgaataata cccttatggg tcgtctcatc gtaaacaggg atattgaact
     1141 cgtttatata atcccttacc accttaatta tggtgtcatt gatgctatgc tgaatatgac
     1201 aagaatccat atcgatgatg ttatggctcc taggagcata aaaccctata gccaacttac
     1261 cccttgacat gccgacagga aactgcatct tgtttcgata atgccatgga tcctccatac
     1321 ccatggtgtc atggactatg acatcctcta ggtttcctat cctttccatg gcatccttaa
     1381 caagctgcct tttaaaatcc agctgcaggt tatatgacat atgctgaagc tggcagcccc
     1441 cgcattgtct tgcatattgg cataggcgct ctacccgctg gttggatgat tttaatatgt
     1501 ctatcagctt tccatatcca aacctctttc ctacctttac cgcttttatc tttacctttt
     1561 ctcccggcag ggccccggat acaaacatgg taaatccgtc tatcctgcct atgccttccc
     1621 cgttagaccc taaattatct atatctatga tatagtcccg attcttaatg accggaatat
     1681 cgttaagttt cctgttattc aagcaaaatg actccttctg ttttaatgag ttatcgttac
     1741 caaaccgcta cttgcccgtc cgtccgaggt tcagttgcac cgcacaaaac gccctcatcg
     1801 tttctccaga ttatttctcc tcttccaaag gttccgctgt tcgccctaac tacgatgtta
     1861 tggccagccc gcataagctc taaagccagc ctattatcaa agccgtgctc tacctctaca
     1921 gttttattgc caacccattg ccaccttggc ctatccaagg cctcctgggg gttcatcata
     1981 taatctatgg tatttaccat aacctgaaga tgggcctgag gctgcataaa tccccccatg
     2041 attccaaaag gtcctacagg tttgcctccc ttagttaaga acccggggat aatggtatgg
     2101 tagggtcgct tgcagcttcc tacgcagtta tccgattcct catccatatt aaagttggca
     2161 cctctgttat ggagtgcgat tcccgtaccc ggtacgacca gacccgaacc aaatcccatg
     2221 taattgcttt ggatgtatga gaccatgttt ccgtctccgt cggcggtgca tagatagacg
     2281 gtgccacctt tggacgggtt gcctgccttt ggcataatag ccttatccgt aatcagcgat
     2341 cttctccccc tggcgtatcc ctcagaaagc agctcatctg tctttatctt cataatcctt
     2401 gggtccgcca cgtacttttg aacatcggta aaggccagct ttagggcctc tatctgaaag
     2461 tgggtgctgt taaaggaatc cttaggacca aggtccatcc cctttaggat attcagcgcc
     2521 atcagcgcgg ttatcccatg gccatttggg gggatctcat atacctcgta ccccttatag
     2581 tctgcactta tgggctcaac ccattccgga taatagtctg ccaaatcatc cttggatata
     2641 aacccaccat actctttaga aaaatcatca atcctctggg ccagttcccc gcgataaaaa
     2701 tcctccccca aggtctttgc aatcctcctt aaggttttcc catgatcctt taatttgaca
     2761 ttatccccaa cacccggaca tatgcccccc ggtgcaaagg tatcaaacca gggttcaaat
     2821 tccttcccct tttttctctc cttatcatag accctatagg atgcctgcca gcctgatccc
     2881 accgtcggtt ggaggataaa tccctcctct gcatacctaa tggcgggctc taaaacctct
     2941 tcaaagggta actttccgaa ccgcttcgat agctccgccc aagccgccgg tatacccgga
     3001 acgttcaccg gaatgaaacc gtattcgggt atttcggtat gtcccaaatt tctgagcgct
     3061 tctaggctta ttgacttcgg agagggtcca ctgctgttta atccatggag cttgtccccc
     3121 gtccatacca gtgcaaaggc atctccgcct attccgttgt ccgtaggttc caccactgtc
     3181 aaggtagttg caacccctat agccgcatca atagcattcc cgcccttttt aagtatttcc
     3241 aggcctacct gggctgccaa aggattacct gtagccacca tgcccttttt accatagacg
     3301 acactcctcc tggatggata gccatacgtc aacggatcaa attgaaattt caaaccccat
     3361 tcccccttag cattttatct aggcggcatt tttgtcgctt ctagccgccc cgtatcctct
     3421 tcaaaccgcc ccgcgacccc cataaaatca cgtgggagct tctagtgtca ttgtactaac
     3481 cgacacatta aatggcaagg agattatttt agaagggcct cctaggatgc catcgttgat
     3541 tatatccgcc ttcggtggta gaattaaaat cgcataatac ataaaaccat atattgtgaa
     3601 aatgaaccat ccattaaaac attagaaatg gggaccggat acttggggac taaaagacag
     3661 attttatcac atgcctttaa gacagctttt ccaaacacca tacctatctt tgcggggttt
     3721 accttcttag ggatggccta tggtatatat atgaacgcat cgggctttag ctttctatac
     3781 cctatgctga tgagccttac catcctggca ggctccatag aatttgttac agtgggtttg
     3841 ctacttggac catttgaccc attgggtgct ttcattttgg cactgaccat caatgcacgc
     3901 cacctcttct atgggatctc catgctggga aaatacaggg tctcgggact taaaaggtta
     3961 tatctgatct ttgggatgtg cgatgaatcc ttctcagtta actatatggc ggatattccg
     4021 gagggtgtgg ataggggttg gtttcaattc tttgtcaccg ccctaaacca tagctactgg
     4081 gtattgggcg caacccttgg gggactcttg gggcatatag tcgaatttga cacaaaggga
     4141 ctggagttta taatgacggc cctgtttata gtgctgtttc taaatcaatg gatgaaggaa
     4201 aaaaatcatc atagctcaat aatcggactc acggtctctg caacctgtct gatccttttc
     4261 gggagcagcg ggtttatcat ccccgccatg gttcttatat tgttgattct caccgtattt
     4321 aaaaacactt ttacaaaggc gggtgttgtt tcatgaccat gaatgtccat gagcacataa
     4381 taaccataat agcggtaatc ctggggacta tggcaacccg ttttcttccc tttatcctgt
     4441 ttcctgccga taagcctacc ccaaaatata tagaatactt aggaaaggtg atacccgctg
     4501 ccgtattggg tttgcttgtg gtgtatagcc ttaaggatgt agaaattttg accggaagcc
     4561 atggaatacc ggagttaatc tccatagcta tcgtagtagt ccttcacatc tggaaaaagc
     4621 agatgctcct ttccatagct gccggaaccg ttgcctatat gctgttggtg caatttatat
     4681 tttaaataac tctgctaata cttattcatg ttgtgtgtat atttatataa tgtatattta
     4741 cacttcgcct ttattggtat aaaatagtat tcatagaacc tttaaataat aggtaaatta
     4801 tttgtacggg agtgaaacag atgaacaaaa gaaacgtaat aattataggc gcagcgggaa
     4861 gggattttca taattttaac acctactatc gaaacaatga tagctataat gttgtggcat
     4921 tcaccgcagc ccaaattcct gatatagaca acagggaata tcccggtgaa ttggcagggg
     4981 gtctatatcc tgaggggata cctatatatt cccaggatag attggccgac cttattgggg
     5041 aactatgcgt ggatgaatgc gtatttgcat acagtgatgt atcctatgaa gatgtcatgg
     5101 gcataagcgc aatagtcaac gctgccggtg cagatttccg gctccttggg cccaagagca
     5161 ccatgataaa gagtacaaaa ccggtaatat ccgtatgttc cataagaaca ggctgcggaa
     5221 agagccagac atccagaagg gtgatcgaac tcttgatggg atttggccta aaggttgtgg
     5281 ccataaggca cccgatgccc tacggcgacc tagcggccca aaaggtccaa aggtttgcta
     5341 cgctggatga cttaaagaga cataagtgca ccatcgaaga gatggaggaa tacgaacccc
     5401 atatagagcg gggcaatatc atctatgccg gtgtcgatta cgaagagata ttaaaggcag
     5461 cagaaaatga tcctgatggc tgtgatgtca tcctttggga tggcggaaat aacgacttct
     5521 ccttctacga gccggatctg tccattacgg tattggatcc ccatagggcg ggccacgagc
     5581 tcagctacta tcccggggag gttaacctta gaaccgccga tgtggcaatt ataaacaagg
     5641 tggacagtgc ggaaaaagat gccgtgcaac aggttgagaa taatatcagg gatgcgaatc
     5701 caaaagctac aataataaag gcggagtcta aaataaccgt gacagagccc gacgttatta
     5761 agggtaagcg ggttctggta gtggaggacg ggcctaccct aacccatggc ggaatgggta
     5821 tcggtgccgg tacggttgct gcccagaggt tcggtgccaa agaggcagtg gatccccgtc
     5881 catttatagt cgggaccctt aaaaagacct ttgaaaacta caagaacata ggaaaacttc
     5941 ttcctgccat gggctatgga gaacagcaac ttaaggatct cgaagagacc attaacaatg
     6001 cagactgtga ttctgtaatc attgggacgc ctattgatct atccagggtc attaatatca
     6061 ataagccgta caccagggtg agctatgaac tcagcgagat tgacaaacca gatctaaaag
     6121 cgatactcaa agattttgtc gacggcctat aaaataatgt gccatgctga ttaaagcata
     6181 ggcatacgaa atttttgttg aattcttaaa ggttatatgg tatcttagag tcgttataaa
     6241 aacacgggtg aagacctcgt ctttgtggaa accctgatag agattcggcg ccgtggctgt
     6301 aagcgctgat taggggagta gtagaacgga gcaacacttc atctataagg aaaaagctta
     6361 tcaaaaccta atagaatgag ttgggcgctt atgcgtcaat gtgggtggca ccgcgggaag
     6421 tcgtctcgtc cctcaaaacg aaatcgtttt gagggctttt tattttaacg caggaggaat
     6481 tgctttggac aagtacagaa cacataattg tggagaattg agaaaaggta atgtgaaaga
     6541 gcgggtacgc ctagccggct gggtcagtac cattcgggat catggcggcg ttatatttat
     6601 tgatcttagg gatcattatg gaataaccca gattgtaata gatgatgaat ccttaataaa
     6661 gggaattggc cgggagtatg taatctccgt tgaaggagtt ttgcgcggca gatcggagga
     6721 aaccataaac cctaaaatag caacgggtga agttgaagta aaggtagatc atatcaccgt
     6781 acttggcact tcggtctcac aattgccctt tgaaatcggg gaatcgacca tgacccgcga
     6841 ggatattcga ttaaggtatc ggtttttgga tctacgaaac ccttccgttc acgagaatat
     6901 cgtaatgcgc tccaagatta caagccatct caggagaaag atggaggatt tagggtttgt
     6961 ggagatacag acgccgattt taacctcgtc gtcccctgag ggggcaaggg attatttggt
     7021 acccagcaga aagcaccatg gaaaattcta tgccctgccc caggctcctc aaatattcaa
     7081 acagcttttg atggcctcgg gctttgatag gtacttccaa attgcgccct gctttcggga
     7141 cgaggattca agagccgatc gctccccggg ggaattctat caactggatt ttgagatggc
     7201 ctttgccacc caggaggatg tccttgccgt ggcggaggag gtgctctatg ataccttttc
     7261 taagttctcc gaaaaaaggg tcagccccgc gccttttaaa cgcatcccct ttgcggaaag
     7321 tatgctaaaa tacggcaccg ataagccgga tctacgaaac cccctaatta tacaggatct
     7381 gacggacttt tttagggatg tagactttgc gccctttaag aataaacccg tcaggggcat
     7441 agtcgcccca aattgcgccg gcatgccccg ctccttcttt gaaaggatgc tagcctttgc
     7501 cacgggggtt ggcatgaagg gcttgggata tatctcggtg ctcccgggta tggagctgaa
     7561 gggccccata gtgaaattcc tttctgcaga aaaacagcgg gagatgattg accttctaaa
     7621 cctgaaggaa gacgataccc tgttctttat ctgcgatacc cccaaaatgg tggataagtt
     7681 agcaggattc atccgcacgg aacttggtga gcgccttgac atcatagatc gagattcctt
     7741 tgagatgtgc tttattacgg attttcctat gtatggttta agtgaaaata caggcaagat
     7801 agaatttacc cataacccat tttccatgcc ccagggtgaa atgcaggccc ttgtggatat
     7861 ggatcccttg gatataaagg cctaccaata cgatatcgtc tgtaatgggg ttgaactatc
     7921 ctcaggggcg gtcagaaacc atcgaccgga tataatgata aaggcctttg aaatagcggg
     7981 ctattgcgaa gaggatatta aagataagtt cccggccctg ttcagcgcct ttcattacgg
     8041 tgccccgccc catgctggta tggctccggg gctcgacagg atggtcatgc tccttacagg
     8101 ggaagagaat atccgggagg tcatagcctt ccccatgaac gccaatgcgg aggacctgct
     8161 cctaggtgcg cccggcccgg ttacggaagc ccagcttcgg gaaattcata taaagatcag
     8221 gtaagagtat gggttgcgcc ggtttaatat gtatagaata aatgaatgaa gggtttttgt
     8281 tgggtatgaa tacgtttgtc aggcagaaag ggagctgaga ttatgccgag gatagaggat
     8341 tatgaaacaa tgacaaagat atccctgacc aaagaagaaa ggggtatagt atccggtcat
     8401 atgaatatgc tgctggacag ctttaaggag cttggggata tagatgtttc aggggttgag
     8461 ccccttgtaa gcgttctgga tataacaaat gatatgcgag atgatgttgc catcaagact
     8521 ttgtctcggg aggagctttt gtcaaacgcc cctgagcagt atgacggcta ctttcaagtc
     8581 ccaagaaccg ttgattagag gagggaagat atgggactct ttatagaaaa ttataatatt
     8641 tcaggtgccg atgccgtggc attggaagac agtatcatgg aaaaaggcag aggtgtgacg
     8701 gtgggatcta ggatcctaaa gaacttcgtc tccccgttta acgcgacggt ggtggacagg
     8761 cttttagata atggattcaa aattgccgga agggctaaca tggatgaatt tgggctgtta
     8821 aaactgtcac gtgatgatga taatatgcct acaggggcta taagagctgt ctcggacgat
     8881 gttgccgcct tttccctatg caatgacctt tttggcaggt atcgaaggga agccaccgaa
     8941 aatgattgct gctatatcca tcccacctat ggaactgtat ccagatacgg cctcgttccc
     9001 ctagcctcgt ctatggatca gatagggata gtatgcaagg atttagccca gggtttcggg
     9061 cttctttcat acatttcggg taaggatcct aaagacggtg ccatgtttcc cgaaagcacg
     9121 tacgactaca ccaaaccggc cggtgaaatt accttggggg tgcccaaatc cgttatagag
     9181 aaggcggatg tagatacaca agatgccatt acggagttta ttgacgggtt tgccaatact
     9241 gagatagacc ttgagcactt cgatctatac agccgggtta tgtatatcct aagctctgcc
     9301 gagatcagca acaacttaag ccgctacgat ggaataaagt ttggataccg ggcctcgaac
     9361 ttttctgacc tcgaagatct ctatacgaaa acccgcagcg aaggcttcgg cccggatgca
     9421 aagtccgcta tcattatggg ggctatgatg ctttctaagg atcagtacgg cgcatattat
     9481 gaaagggcca tgaaagtccg tcgtctaatt aaggaatcca taaagtttga taggtataac
     9541 gtcattgtcc tgccatgtaa tataggtgat gacccatatg agaacctttc cctttatgca
     9601 ctatcccccc tggcgggcct gccatcggtt tccttttcct ataagggcca cggcatacag
     9661 cttatcgcag gcgtaaagaa cgaggatctt ctattatcca cctgggaggt gtgtaaggcg
     9721 tgaaatacga aacaataatt ggtcttgaag tccatgtaga actggctacg aaatccaaat
     9781 tattctgccc ctgttctaca gaattcggct cggatatcaa tgagaacgta tgccctatat
     9841 gttctgggat gcccggaaca tcccccatga tcaacaaaaa agccgttgag ctgggtatag
     9901 ccgccggact tttgaccaac agtgccattg cccgaaggat tgccttcgat aaaaagaatt
     9961 acttttatcc cgatctccca tcgggttacc aggtcactca gctcttttct cctatctgta
    10021 aaaatggctg ggtggagata gaaacagatg cgggcaaaag gaggattaat ctaacgcaga
    10081 tccatataga agaggatgcc ggaaagctca ttcatgacga ctggacggac accaccttag
    10141 ttgactttaa ccgggcatcg gttccattaa ttgagatagt aacaaatcct gattttcgat
    10201 ccggggaaga ggtagttgct ttcttagaaa aactccgttc cctgctcagc ttcgcggggg
    10261 tatccgattg caaaatgcag gaaggctcta tgagatgcga tattaacctt tctgtccgtg
    10321 aaaagggcac agaaaagctg ggtaccagaa ccgagattaa aaatatgaat tcattaaagg
    10381 ccattaccgc agccattgcc tacgaatcta gaaggcatat agaggccctt gaaacgggag
    10441 acgaggtcct ggttcaagag acccgcaggt gggacgatag tataggcgag accttttcca
    10501 tgcgcgggaa ggaggatgcc accgattatc gcttttgtcc tgaccctgat attatgccgg
    10561 tctttatagg tgatgagtgg atagactctg tccgcgataa tcttcccgaa acggcccaag
    10621 caaaatttat taggctgact gaggagcttg acctttccga atatgacagc gaaataatta
    10681 caagaagcaa aaacctgtcg gatatcttcg atgaaacctt aaagcacttt gaaaatccta
    10741 aggaggttgc caactgggtt atagtcgaac ttctggctat cgccaagggg gacaataaga
    10801 acgaagatga tgtgagaatt gactgccgaa agtttgcaaa gcttatagag ctggtggaga
    10861 ataagaccat caaccgcaat gtaggcaagc aagtgctctc ccttgtgctg gaagatgata
    10921 ttgatcccgt ccaatatata g
//
LOCUS       DASRNX010000117        11102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_447858,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000117 DASRNX010000000
VERSION     DASRNX010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11102
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447858"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VFD89_04705"
     CDS             complement(<1..759)
                     /locus_tag="VFD89_04705"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HZJ57523.1"
                     /translation="MRFNHRDDIIQITPLWKGERMEDGRPKVPSDILRRLRKITLEEA
                     WGTLWRYGYRYQFEGDFKVINPDMVLVGRAVTGVMVPYRPDLNDTLLNYGHQEEGRSG
                     FFNQWIIDSLVEDDVVVIDMFDKIYQGTFVGGNLTTAIAARTKRGGAVIWGGVRDTEQ
                     MMAIKNVKTYHRGNDPTGIGDVTLVGYNGPCRIGKAICLPGDVVLGTPAGVLFIPAHL
                     AEECTIEPKNPISEIFGDSRCLTRGNIPQLKSTQA"
     gene            1290..1598
                     /locus_tag="VFD89_04710"
     CDS             1290..1598
                     /locus_tag="VFD89_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57524.1"
                     /translation="MLRVQRYVNDIRKIIEKTEVYGREMSIIGKYLKINIGANIRNYL
                     DMIYDRRGFTREELIIIIREFDEYLDDSLMDEYGEHIDSYPQGSRDEISGMGHDSSGV
                     "
     gene            1769..3217
                     /locus_tag="VFD89_04715"
     CDS             1769..3217
                     /locus_tag="VFD89_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZJ57525.1"
                     /translation="MIELDKAGPIGKILNSRKFGAALAIIQSAITLIFLATVINLNII
                     PSKYFIPMMAVLLVSLGFTFLSQLFLKNRKFGKILAIFMCLILGLACYYLIKTQFLLT
                     DISSKNTKIDDVSVIVLKDDPAQSISDAADYLFGIQDVLDRENTDKTIEKINGDLDIV
                     MKTEAYDDFSSQVEALYGGQVDAIILNEAYRDTIREAYEDFDEMTRVLTSHQIETSVE
                     IEPGDKKVTAEPFNVYISGIDTYGSIRRTSRSDVNIIATVNPKTKEILLTTTPRDYYV
                     PFPNTDGVRDKLTHAGVYGIDVSIGTMENLYGINIDYYVRVNFDTLIGMVDALGGISV
                     DSEYAFKAGGYNFEKGKNQLNGAQALAFSRERYAFAAGDNQRGKNQMAVIKGMIDKAV
                     SPAVVTNYSSIMDSISESFETNMSPNDITSLIKMQVNDMSPWKTETNNVTGKGAMKTT
                     YTYKKRPLYVAIPDESSVEAARDLIGQVYGGN"
     gene            3425..5122
                     /gene="polX"
                     /locus_tag="VFD89_04720"
     CDS             3425..5122
                     /gene="polX"
                     /locus_tag="VFD89_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HZJ57526.1"
                     /translation="MDKAKVVEVLNEIALLLRLKGDSTFRIRAYEKGARVIEFLEEDL
                     HTVVQEKRLNQIKGIGKALEQNIEELVTTGSSAYHKELMEEFPETLFELFKVPGLGPK
                     KVQTLYDKLGITNLGELEYACLENRLLILPGFGEKTQQNILQGIADLKHYQSNFLYSE
                     ARSIAERIVEEMLKQPGVTTAHAAGSLRRRKEIVGDIDILASSLWPVSLMDWFTSLDY
                     VDTVTGKGETKSSVKLTNGMNADLRVVSPEQYPYALHHFTGSKEHNTAMRHIARSRGI
                     RMNEYGLFEVEDETLIPCKDEEEIFRFFGMDYIPPELREDLGEIQAAMEGNLPKLVGA
                     EDIRGILHVHTTYSDGRSTLKEMADEAIRLGYDYIGISDHSQSAYYANGLSPEDIKKQ
                     HEEIDELNGLYNGRIRILKGIESDILPDGDLDYPDEILDTFDFVIASVHSGFSMDRDT
                     MTERLLAAMDNPYTTILGHPTGRLLLSREGYPLDMEAILEGAAKRGVAIEINANPHRL
                     DLDWRWCRKAKELGVLLAICPDAHDIDGLSHISYGINIARKGWLTADDIFPYPFPKR"
     gene            5160..5984
                     /locus_tag="VFD89_04725"
     CDS             5160..5984
                     /locus_tag="VFD89_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZJ57527.1"
                     /translation="MKFKVALQIYSVRDFAEKDLNGTLKKIKEMGYDGVEFAGLYGHE
                     PSEIKSMLDEIGLEPVSAHVPIEEMLDDPKKTIGAYAKVGCRYIAVPYLVEERRPGTD
                     GFMQTIKDIETVAKVAKDFGIQMLYHNHDFEFIKVDGEYALDLLYRSVSADLLKTEID
                     TCWVNVAGEDPADYIRKYTGRSPVVHLKDFVMDDREKPGKLYELIGIDEEDGEGEGTF
                     EFRPVGYGVQDFPSILKASEEAGTEWVVVEQDQPSLNKTSLECAAMSREYLKEIGL"
     gene            6394..7611
                     /locus_tag="VFD89_04730"
     CDS             6394..7611
                     /locus_tag="VFD89_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ57528.1"
                     /translation="MTEDISIVLVGIGGYGGVYLEDLLEGEPEEGIKIAGAVDPNPAG
                     CKYLDQLKRLQIPIYESLEDFYAEHSADLAIISSPIHFHSPQTRLALSNGSNVLCEKP
                     VSATIQEAREMAEARDKFNKFVAVGYQWSHSPVIHDLKKDIMEGTLGKPKILKTMVLW
                     SRPDDYFNRSWWAGKIRDSSRNWILDSVANNATAHYLHNMFYVLGDAIDQSATPAEVT
                     AELYRANAIENFDTAAIRAKTEEGTDILFLATHAVHIHPMPQFCYEFENAKVVFCDPD
                     IPESENNILAIFHDGRTKAYGDPNKSDRVRKMWMAVDAIREKAEIVCGIEAASSHTVC
                     INAAHESAGEIVNFPPDLTRRDEDRRINWVEGLKETFRQCFAENRLPSESGIPWAKPG
                     KTIDTGGYDFFRG"
     gene            7660..8673
                     /locus_tag="VFD89_04735"
     CDS             7660..8673
                     /locus_tag="VFD89_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015807515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZJ57529.1"
                     /translation="MGLERFGNKLTEPSQGLIEDFNRIDGDILILGMGGKMGSDLGLL
                     AKRAIDESGGSRRVIGVSRFSDGKIREGLKSQGIETISCDLEDERQLAGLPQVKNVIY
                     MVGKKFGTTGDEHTTWGINTYLPGGVAEKYKDSKIVVFSTGNVYPLTRVGSGGSCEED
                     DPDPVGEYAQSCLGRERIFEYFARRNGTQMAFLRLNYANDLRYGVLYEIAKSVREGRA
                     IDLSTGHVNLIWQGDANEYALRAFNHCTCPPNIINAAGPEIVSIRWLATLFGKGFEKE
                     PIFINKEGDTALLSNGSKGHRLFGYPRVTLRDMVEWQIEWLNVGGEVLDKPTHYQERG
                     GNF"
     gene            8676..9779
                     /locus_tag="VFD89_04740"
     CDS             8676..9779
                     /locus_tag="VFD89_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZJ57530.1"
                     /translation="MDHTKSINPTKRINPEVYNLLHEGTVIPAHPLALNSARQLDERR
                     QRALTRYYMDAGAGGIAVGVHTTQFEIRDEGVDLLEPVLRLASEEVESANLDRSFIRI
                     AGICGPTDQAVEEAKLAARLGYDLGLLSPGGLGDWTEGEIIERAFKASEIIPIFGFYL
                     QPAVGGRNLSYEFWRDFAQIPGVMAIKIAPFNRYQTLDVVRAVCDSPRRNEIALYTGN
                     DDNILGDLLTAYRFVVDGQLVVKRIVGGLLGHWAVWTRCAVEVMDAVKKLRTESDASG
                     SGHNIGDMLATGTGITDANAALFDASNDFKGCIPGIHEVLRRQGLLEGRYCLNPDETL
                     SPGQLEEIDRVYRDYPHLNDDDFVRENLDKWLR"
     gene            9868..>11102
                     /gene="glgB"
                     /locus_tag="VFD89_04745"
     CDS             9868..>11102
                     /gene="glgB"
                     /locus_tag="VFD89_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HZJ57531.1"
                     /translation="MTNKFLTQEEIFLFNEGTFYNCYLKFGAHRIRLGGTWGVHFALW
                     APNASLVRVVGDFNHWEGKDHTMRLRKGGIWELFIPHQEAGEVYKYEIITAEGDTLLK
                     ADPFAFYSELRPDTASIVYPLSGYSWGDGQWMIKREESLAKNSPLNIYEVHLGSWRRK
                     EDDSLYSYRELADLLIPYVKRLGFTHIEILPLMEHPFDGSWGYQLTGYYSATSRYGSP
                     HDLMYFIDRCHQEGLGVILDWVPGHFCRDSHGLGVFDGLPLFEGGDHQEWGTYEFDFS
                     RLEVWSFLIGNAVFWLDKFHMDGLRVDGVTSMLFLDYGKGDSPWKPNIHGGRENLEAI
                     DFLQTLNEIVHKLYPGSLMIAEESTDWASVTKPPEDKGLGFDYKWNMGWMNDTLNYMK
                     TDFSGRMDHHNKLTFSLYYA"
BASE COUNT         3229 a         2286 c         2883 g         2704 t
ORIGIN      
        1 agcctgcgtc gatttgagct gtggaatatt tcccctcgtc aagcatctgg aatccccaaa
       61 tatctcagat attggatttt tcggctcgat agtacattct tcagctaagt gggcggggat
      121 gaacaatacg cctgcagggg tacctaaaac aacgtcacct ggcaagcaga tagctttacc
      181 tatacggcaa ggtccgttat agccgaccag ggtaacatcg cctatacctg taggatcgtt
      241 accgcggtga taggttttga cgttcttgat agccatcatc tgttcagtat ctcgtactcc
      301 accccagatt acagctccgc ctctcttagt gcgggcggcg atggccgttg tgaggtttcc
      361 acctacaaag gttccttgat agattttatc aaacatatca atgacaacaa cgtcgtcttc
      421 aactagagag tcgatgatcc attggttaaa gaaaccggaa cggccttcct cttggtgtcc
      481 ataatttaga agtgtatcgt ttaagtcggg acgataggga accataacac ctgtaacagc
      541 tcgacccact agaaccatgt ccgggttaat tactttaaaa tcaccttcaa actgatatct
      601 ataaccatag cgccataatg ttccccaggc ttcttcgagg gtgatcttac gcaatcttcg
      661 aagtatgtct gacggtactt ttggtcttcc gtcttccata cgttcacctt tccataaggg
      721 cgttatttgg ataatgtcat cgcggtgatt aaatctcatc ttcgcattcc tcctataaca
      781 tttgggttgt ttatttttca caagcaccac aatattaaag attatggcac tgtatgaatt
      841 cgtttaaaag gtgtaggtat gtaatttttt gacttaagat actattcgtt atggggcact
      901 gaactttact gctcggaaaa ttcctatgaa aagtttaaaa taatatatta caataggcct
      961 gcttattttt caaagcgaaa acgatggtat atcccgtaca ttttatgccg aggatttcgt
     1021 agtttatgac aagcatctca catcggaatt catcgggcac aaaacatttt tatgacttat
     1081 atgctacgaa aaattaataa taatttacaa ataggttttt tctgtaacat atatttataa
     1141 ttataggaca gcacttataa aaagtcaagc tgccttattt atttccagta gagggatata
     1201 gcgccaaaag gccttataaa ccagcggaca gggcggagag tataatccgg ggaaaaatgc
     1261 agcaaaataa caccaaggtg gtgaattttt tgttgagagt tcaaaggtat gtaaacgata
     1321 taaggaagat aattgagaaa accgaggttt acggcaggga aatgagcata atcggcaaat
     1381 atcttaagat aaatattggt gctaatatac gcaactacct agatatgata tatgatagaa
     1441 ggggctttac ccgagaggag ctcatcatta tcatcagaga atttgatgag tatttagacg
     1501 acagcctgat ggacgagtat ggggagcata tagactccta tccccaggga agtcgggatg
     1561 aaatcagcgg tatgggccat gattcaagtg gggtctagaa aggtcaaatg aacaggcatt
     1621 gtgtacagat tttggggttt gattaaaata tttgccggcc gaactttcac gatatggcta
     1681 cgggcaaaaa cgcctattgc tagcggtgtc ataagatgtt attatatagt gaacattaac
     1741 caaagattca aaaattgggg gtaatcaaat gatagaatta gataaggccg gtcccatagg
     1801 taaaatttta aacagcagga aattcggagc cgctcttgca attatccaat ccgcaataac
     1861 ccttattttt ttggcaacag taatcaatct gaatataatc ccgagcaaat actttatccc
     1921 gatgatggca gttcttttgg tctctctcgg ctttaccttc ctgagccaat tatttttaaa
     1981 gaatcgaaaa ttcggaaaga tccttgcaat atttatgtgt ttgatactcg ggctggcgtg
     2041 ttattatcta attaagaccc aatttttgct tacggacatc tctagtaaaa acaccaagat
     2101 agacgatgta tccgtcatcg tattaaagga tgatcccgcg cagagtataa gcgatgcagc
     2161 agattatctt tttggtatac aggatgtctt agatagggag aacaccgata agaccataga
     2221 gaagataaac ggggatctgg acatagtgat gaagaccgag gcctacgacg attttagctc
     2281 ccaggtggag gccctttacg gcggacaggt ggatgctata atattgaacg aggcgtatag
     2341 ggatactata agggaagcat acgaggattt tgatgaaatg acgagggttc tgaccagtca
     2401 tcaaatcgaa acatcggtgg aaatagagcc cggcgacaag aaagtgaccg cggagccctt
     2461 taacgtatat ataagcggta tagataccta tggatccatc agaagaacca gcagaagcga
     2521 tgtcaatatt attgccacgg tgaaccctaa gaccaaggag atattgttga ctacgacccc
     2581 aagggactat tatgtgccct tccccaatac ggatggggta agggataaat tgacccatgc
     2641 cggagtctat gggattgatg tctccattgg gacaatggaa aacttgtatg gtattaatat
     2701 agattactat gtacgggtaa actttgatac ccttatagga atggtagatg ccctaggagg
     2761 aatcagcgta gattcggaat acgcttttaa agcaggggga tataacttcg aaaagggtaa
     2821 aaatcaatta aacggagccc aggccttggc cttttccagg gaacggtatg cctttgctgc
     2881 gggagataac cagcggggta agaaccaaat ggcggttata aaaggtatga tcgataaggc
     2941 cgtatcgccc gccgtagtaa ccaattatag cagcatcatg gacagcatat cggagagctt
     3001 cgagaccaat atgagtccca acgatattac cagccttatc aaaatgcagg taaatgatat
     3061 gtccccatgg aagaccgaaa ccaacaacgt aacgggaaaa ggggcaatga agacaaccta
     3121 tacctacaag aagaggcccc tgtacgttgc tatacctgat gaatcctctg ttgaggctgc
     3181 cagggattta ataggtcagg tttacggggg aaattaagga tcacgtatat gttttaagac
     3241 aaacattttt cactggtata cgggtgtcgt aaaacattat ccttaatcca tatgttacaa
     3301 aaaatacgtt gctttaacgc aaaattggac aattagctga ttctgttacg aaagatcctt
     3361 ttcgtaacac gtatagatga aagaaccata ggtaggcggg aggcccgaag gaggttttaa
     3421 gcttatggat aaagccaagg tggtagaggt attaaacgag attgcccttt tgcttcgtct
     3481 gaagggcgac agcaccttta ggattagggc ctatgaaaaa ggtgcccgag tcatagagtt
     3541 tcttgaagag gaccttcata ccgtggtaca agaaaagcga ttgaaccaaa taaaagggat
     3601 cggtaaggcc ctggaacaaa atatcgagga gctggtcacc accgggagct cggcctatca
     3661 taaggagctc atggaggaat ttcccgaaac cctctttgaa ctgttcaagg tgccggggct
     3721 ggggcctaaa aaggtccaga ccctctatga taaacttggt atcacaaatc tgggagagct
     3781 ggaatatgca tgcctcgaaa atcggctttt gattcttccc ggatttgggg aaaagaccca
     3841 gcagaatata ctgcagggca tcgctgatct caagcattac cagagcaatt ttctctattc
     3901 cgaagcccgg tccatagccg aacgcatagt ggaagagatg ctgaagcagc ccggagtcac
     3961 aacggcccat gctgccggga gccttagaag gcgtaaggaa attgtagggg atatagatat
     4021 attggccagt agcctttggc ccgtaagcct tatggattgg ttcacttcct tagactatgt
     4081 agacaccgtc acgggcaagg gggagaccaa atccagcgta aaactgacaa atgggatgaa
     4141 tgcggatctg agggtggtat caccggagca atacccctat gccctgcatc attttaccgg
     4201 gagcaaggag cataataccg ccatgcgaca tatagcacgc agccgcggaa ttaggatgaa
     4261 cgaatacggt ctgtttgagg ttgaggatga aaccctaata ccctgtaagg acgaagagga
     4321 gatattcaga tttttcggaa tggattatat tcccccggaa ctaagggaag atctgggcga
     4381 gatccaggct gctatggagg gtaatcttcc gaaactggta ggggcggagg atataagggg
     4441 cattcttcat gtacatacca cctatagcga cggacggtct accttaaagg agatggcgga
     4501 tgaggccata aggctgggat acgactatat cgggatatcc gatcatagtc agtccgccta
     4561 ctatgccaac ggcctttctc cggaggatat aaaaaagcag cacgaagaga ttgatgagct
     4621 gaatggccta tataatggaa ggattagaat cctaaagggg attgagagcg atattctccc
     4681 ggatggcgac ttggattatc cggatgaaat cctggataca ttcgattttg tgatagcatc
     4741 cgttcattcg ggattttcca tggacagaga caccatgacg gaaaggttgc ttgcggctat
     4801 ggataacccg tatacaacca ttttggggca ccccaccggg cgcctacttt tatccaggga
     4861 aggctatccc ttggacatgg aggctatact ggaaggggcc gccaaacgag gcgttgctat
     4921 tgaaatcaac gcaaacccac atagattgga tttggattgg agatggtgtc gcaaggccaa
     4981 ggagctgggg gttttgctgg cgatttgccc tgacgcccat gatattgatg gtctaagcca
     5041 tatatcctat ggtatcaaca tagccagaaa aggatggctg acagccgatg acatattccc
     5101 atacccgttc ccaaagaggt aggggttacc ataaattaaa gaagcaggag gaacaaatta
     5161 tgaagtttaa agtagcgcta cagatctatt cggtaaggga ttttgcagag aaggatttaa
     5221 acggtacatt aaagaagata aaagaaatgg gctatgatgg tgtggaattt gcagggcttt
     5281 atggccacga accgtcagag attaagagta tgttggacga gatcggccta gagcccgttt
     5341 ccgcccacgt tcccatagag gagatgttgg atgatcctaa aaagaccata ggggcatatg
     5401 cgaaggttgg atgcaggtac atagccgttc catacttggt agaggaacgc aggcccggga
     5461 ccgacggatt tatgcagacc ataaaggata tagagacggt ggcaaaggta gccaaagatt
     5521 ttggaatcca gatgctgtat cacaaccacg atttcgaatt tataaaggtg gacggggagt
     5581 atgccttgga ccttctctat agatccgtat cggccgacct ccttaagacg gaaatcgata
     5641 cctgctgggt taatgtagca ggcgaggatc ccgctgatta tataagaaaa tatacgggaa
     5701 gatccccggt ggttcatctc aaggattttg ttatggacga cagggaaaag cccggcaagc
     5761 tctacgagct aatcggtatt gatgaagagg atggggaagg cgagggaacc ttcgagttca
     5821 ggcctgtagg ctacggggta caggatttcc cgtcaatttt gaaagcttcg gaggaggccg
     5881 gcaccgagtg ggttgttgta gagcaggatc agccatctct aaacaagacc tctctggaat
     5941 gcgccgccat gagccgagaa tacctcaagg aaataggact atagaacaca aggggactaa
     6001 acacaagggg acggtccttc tgtgttcggg gaacacaaat ggatacagtg gatgtagtcc
     6061 atatgtgttt ttaataaagt atattgaagt atatcaaggg ggttgtggct ttttcgctgc
     6121 agctcccttt tcgctacatc cccccttata atgcatcccc cctttatatt gcagcccttc
     6181 ttttatggaa tttcaagata gctttggcag gattaaagag gcgcatgtag aataatgtat
     6241 tatggtcgta acttaagttc actcgttgtc aataacccat ggctaaagtc acgggtatcc
     6301 acgccgaaat tatgaaaaat gcattgctgc aaaacaaaac tatagaaaag ctagacgata
     6361 taattgatcc tataacgaag ggagatattg aatatgacag aagatatatc aattgtattg
     6421 gtaggtatag gcgggtatgg tggcgtctac cttgaagacc tattggaggg ggaaccggaa
     6481 gagggaataa agatagctgg ggcagtcgat cctaaccccg caggctgcaa atatttagac
     6541 caattaaaac ggctacaaat ccccatatat gaatcattgg aggactttta tgccgagcat
     6601 agtgcagacc tggcaattat atcgtcccct atccactttc attcacccca gacccgtttg
     6661 gccctgtcca acggaagcaa tgtactgtgc gaaaagccgg tgtcggctac cattcaagag
     6721 gctagggaga tggcagaggc aagggataag ttcaataaat ttgtagcggt tggctatcaa
     6781 tggtcccaca gcccagtaat ccatgatctc aaaaaggata ttatggaagg gacattggga
     6841 aaacccaaga tattaaagac catggtatta tggtcaaggc ccgatgatta ttttaacagg
     6901 tcctggtggg ccggtaaaat ccgcgattct agccgcaatt ggatcttaga tagtgtggcc
     6961 aataacgcca ctgcccatta ccttcataat atgttctatg ttctgggaga cgcgatagac
     7021 caaagcgcaa cgcctgctga agtaacagcg gagctttaca gggcaaacgc aatagaaaac
     7081 tttgatacag cggccataag agcgaaaacc gaagaaggta cagacattct attcctggct
     7141 acccacgccg ttcacatcca tccaatgccc caattttgtt atgaattcga aaatgccaag
     7201 gtagtatttt gcgatcccga tatcccggaa agtgagaata atatcctggc tatatttcat
     7261 gacggcagaa ctaaggcgta tggggatcct aataagagcg atagggtaag gaaaatgtgg
     7321 atggctgtag atgccatcag ggagaaagcc gaaatcgtat gcgggataga ggccgcatct
     7381 tcccacaccg tctgcattaa cgcagcccac gaatccgctg gggaaatagt aaatttcccg
     7441 ccggatttaa caagaaggga tgaagacagg agaatcaact gggtagaggg tctaaaggaa
     7501 acctttagac aatgctttgc agagaacagg ctcccatcgg aaagcggaat cccttgggca
     7561 aaaccgggaa aaaccattga tactggggga tatgatttct ttaggggata accccatatc
     7621 tcttataaca tcaatcaact ggaaggggcg atggggatta tgggtcttga aaggttcggg
     7681 aataagttga cggagccgtc ccaagggctt attgaagatt ttaaccgaat agacggggac
     7741 attctcatcc taggaatggg cggtaagatg gggtcagatt tgggcctatt ggctaagagg
     7801 gccatagacg aatcaggggg atctcgcagg gtgatagggg tctcgcggtt ctcggatggg
     7861 aagataaggg agggcctaaa aagccaaggg atagagacta taagctgcga tcttgaagat
     7921 gaaaggcaac tggccggcct accccaagta aagaacgtta tatatatggt agggaaaaaa
     7981 tttgggacca ccggcgatga acatactacc tggggaataa atacctatct tcccgggggg
     8041 gtagccgaaa aatacaagga ttcaaagata gtggttttct ccacgggaaa cgtgtatcca
     8101 ttgacccggg ttggcagtgg tggatcatgc gaagaggatg atccggatcc cgtaggagaa
     8161 tatgcccagt cctgcctcgg gcgtgagaga atatttgaat acttcgcccg taggaacgga
     8221 acccaaatgg catttctgag gcttaactat gccaatgatc tcaggtacgg ggttttatat
     8281 gagatagcaa aatccgtcag ggaaggcagg gctattgatc ttagcaccgg ccatgtaaat
     8341 ttaatatggc agggcgatgc caacgaatat gccctaaggg catttaatca ctgtacctgt
     8401 ccgccaaata ttataaacgc agcggggccg gaaatcgttt caatacgctg gctggccacc
     8461 ctttttggaa agggctttga gaaagaaccc atctttataa ataaggaagg ggacaccgcc
     8521 cttttaagca acggttcaaa ggggcatagg ctctttggat atcctagggt aaccctcagg
     8581 gatatggtag agtggcagat agaatggctt aacgtcggcg gggaagtgct ggataagccc
     8641 acccattacc aggagagggg aggcaacttc tagatatgga tcatacaaaa agtatcaatc
     8701 ccacaaaaag gatcaatccg gaggtatata atttgctcca tgagggtacc gtcattccgg
     8761 ctcacccatt ggccctaaac agcgcaagac agctggatga aaggcggcaa agggccttga
     8821 cccgttacta catggacgca ggtgcggggg gaatagcggt aggggtccat acaactcagt
     8881 ttgaaatcag ggacgagggt gtggacctgt tggaacctgt cctgaggctt gcatcggaag
     8941 aagtggaaag tgcaaacctc gatcggtcct ttattcgcat tgccggcata tgcggtccga
     9001 cggaccaggc tgtagaagag gcgaagctgg cggcgaggct cggctatgac cttggtcttc
     9061 taagccccgg gggcctgggt gactggacag agggagagat aatagagcgg gcctttaagg
     9121 catcggagat tataccgata tttgggttct atctccagcc tgcagtgggc gggagaaatc
     9181 tttcctatga gttctggagg gactttgcac agattccggg ggtgatggct atcaaaattg
     9241 ccccatttaa tagatatcag accctggatg tggtaagggc ggtatgtgat tccccaaggc
     9301 ggaacgagat cgcactctac accggaaacg acgataatat tctcggggat cttctgaccg
     9361 cctacagatt tgttgtggac ggacagctgg tagtaaaaag gattgtaggc ggactgttgg
     9421 gacactgggc ggtctggacc cggtgcgcag tagaggttat ggacgctgtc aaaaagctaa
     9481 ggactgaatc cgatgcatcc ggcagcggtc ataacatagg ggatatgctg gcaacgggaa
     9541 caggcataac cgatgcaaac gcggcacttt tcgatgcttc caacgatttt aagggctgta
     9601 tacccggcat acacgaggtt ttaaggaggc agggtctact tgaagggcgt tattgtctaa
     9661 accccgatga gaccctttca cccggacagt tggaggagat cgacagggtg tacagggatt
     9721 acccccactt aaacgatgac gattttgtga gggagaacct ggataagtgg ctcagatgac
     9781 cgaatacaga agacacaaaa ggaccgtccc cctgtgttac ggtcccgctg tgtttacccc
     9841 tgtgttattg gaaaggagga gtgagcagtg acgaataaat ttctgacgca agaggaaatc
     9901 ttccttttta atgagggtac cttttataat tgctatctaa aatttggtgc ccataggata
     9961 aggctgggcg gaacatgggg tgtgcacttt gccctatggg ctcccaacgc ttccctggtg
    10021 agggtagtgg gcgattttaa ccattgggaa ggcaaggacc acaccatgag acttagaaaa
    10081 ggcgggattt gggaactctt catccctcac caggaggcag gcgaggtcta caaatacgaa
    10141 attataaccg ccgagggtga taccctcctc aaggcagatc ccttcgcctt ctattcagag
    10201 cttaggcccg acaccgcatc catagtatat cccctcagcg ggtattcgtg gggggatggc
    10261 caatggatga taaaacggga ggaatctttg gcaaaaaact ccccccttaa catctacgag
    10321 gtacacttgg gatcttggag acggaaggaa gatgacagcc tatacagcta tagggagttg
    10381 gcagacctac tgatccccta tgtaaagagg ctggggttta cccatataga aatcctgccc
    10441 ctgatggaac acccctttga cggatcctgg ggttatcagc ttacgggata ttattccgcc
    10501 accagcagat acggttctcc ccacgatctc atgtatttca tcgatcgatg ccatcaagag
    10561 ggcctaggcg taatattgga ttgggtgccg ggacactttt gcagggatag ccacggcctg
    10621 ggggtatttg atggccttcc cctcttcgag ggtggcgatc accaggaatg ggggacctat
    10681 gaattcgatt tctcccgcct tgaggtttgg agctttctca taggcaacgc cgtgttttgg
    10741 ctggataaat tccatatgga cgggctgagg gtggacggcg tcaccagcat gttgttcctt
    10801 gattacggca agggggacag cccatggaaa cccaatatcc acgggggcag ggagaactta
    10861 gaggccatag actttctcca aaccctaaac gaaatcgtac acaaactcta tccgggatcc
    10921 cttatgatag cggaggaatc cactgattgg gcatcggtca caaagccgcc ggaagataaa
    10981 ggtctgggat ttgactataa gtggaacatg ggctggatga acgatacctt aaattatatg
    11041 aaaacggatt ttagcggcag aatggatcat cacaataagc tgaccttctc cctttattac
    11101 gc
//
LOCUS       DASRNX010000118        27736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_289815,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000118 DASRNX010000000
VERSION     DASRNX010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 27736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27736
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289815"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..2628
                     /gene="adhE"
                     /locus_tag="VFD89_04750"
                     /gene_synonym="adhC"
     CDS             10..2628
                     /gene="adhE"
                     /locus_tag="VFD89_04750"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HZJ57532.1"
                     /translation="MMKAEVNRITEHKDTNEMDIKATIDALVGKGNRALSQFMTMEQE
                     QVDRIVKAMALAGLDQHMKLAKMAIEETKRGVYEDKIIKNLFATEYIYHSIKYERTVG
                     IIDENDLEGYVEVAEPVGVIAGVTPVTNPTSTTMFKALISIKTRNPIIFAFHPSAQDC
                     SSRAAKVMLEAAIAEGAPRDCIQWVETPSLEATRGLMNHPGISLILATGGSGMVKSAY
                     STGKPALGVGPGNVPCYIEKSAEIKRAVTDLILSKSFDNGMICASEQAIILDREIADE
                     AMDFMKENSCYFLYGEELIKVEDMVINKEKGAVNPVIVGQSAFEIAKLSGVKVPEDTK
                     ILVAPIDGVGPDFPLSREKLSPVLALYIVEGYEEGIKRAEEMVAFGGMGHSAVIHSED
                     ERVIDLFSERIKAGRLIVNSPSTHGAIGDIYNTNMPSLTLGCGSFGQNSTTSNVSAVN
                     LINKKRVARRRVNMQWFKIPQRVYFESGSTQYLEKMPDISRAFIVTDPFMVELGYVDR
                     VLYYLRKRRDYVHCKIFSQVEPDPSIETVKRGTAEMDEFKPDVIIALGGGSAMDAAKA
                     MWLFYEYPETDFTALRLKFMDIRKRTFKFPKLGQKTQMVAIPTTSGTGSEVTSFTVIS
                     DNEKNMKYPLADYELTPDVAIIDPDFVMTVPPRITADTGMDVLTHAIEAYVSIMASDY
                     TDAMAMKAIQLVFEYLPKAYRDGSDRLAREKMHNASCIAGMAFTNAFLGINHSLAHKL
                     GGEFNIPHGRANAILLPYVIEYNAQKPTKFASFPKYGYYVADEKYAEIAKYLGLPASN
                     KEEGVASLVKAIRVLMKELDVPLTIAQCGIPENAFEAKVEELADNAFEDQCTTANPRM
                     PLVKELEEIYRRAYTG"
     gene            3137..4405
                     /locus_tag="VFD89_04755"
     CDS             3137..4405
                     /locus_tag="VFD89_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZJ57533.1"
                     /translation="MRLLKPHRNWTFAIIYFFAWAAYATFTLDFIFYSQQIGFNNIEI
                     GILYSTLGLIGIMSQPIFGYISDYFQSTRKVLMGSLTFGIGLSMLLPFVKDKMTVRII
                     AMVYTVFMSSFMPLLDNWVASSCIGEQKGNYGYIRLWGSVGYAIMAFVYGKLTMSIDV
                     SNIYFSRGALFVIVFIFIYTFRHEDMLDKNEVMSDRHEGILESERKKDRPNIKGLFSN
                     KEYILFIIFAFVFSFPLNAASTFFPSLLLEIGGTNATIGFAGSINAIVEIPFFLYTQK
                     LGKRMGHKGLMFVGCVFMCMRMFGFIFAKSIPGLMVAYFFMAPYTSFFAPGMIYYIHS
                     ISPPNTQVVAQTIIQAFAMGSAGMIGNYLAGYFIDSYGIRAMYKYGTVICIAAVVFFF
                     LSGWIIGRLKGGQIGIEVQKKNMIANEHEV"
     gene            4486..5526
                     /locus_tag="VFD89_04760"
     CDS             4486..5526
                     /locus_tag="VFD89_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HZJ57534.1"
                     /translation="MKKLYGYMAGTNLGGWISQYRELNHDHFKSFIVEEDIALIASWG
                     MDHIRVPIDYPVLEEDVNPGVYLESGFQYLDNCIEWCKKHGLNLVIDVHRAPGFSFNT
                     LDKNTLFTDPKMQQRFVDLWIAIARRYAGESDNIMYELLNEIVEPDSLRWNDLATRLI
                     EGIRKVDKKHRIVVGGIDYNSVNALKDMPIFDDPNIVYNFHMYEPFALTHQRASWSKE
                     AMEFDRTMRYPDESLQDYIDFANFTPSRGLVNQYEGLTRMDVNYIRKFLKPAIEFIDK
                     HDLPLYCGEYGVIDHADMASREAWSEDLAEVLLEYGIGRAVWTYKSMSFAHIDGDRNP
                     LSQRLIRAVSRH"
     gene            5574..7109
                     /locus_tag="VFD89_04765"
     CDS             5574..7109
                     /locus_tag="VFD89_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HZJ57535.1"
                     /translation="MGMIFEYDEVPSVQTKSGKIKGYAYNGVHIFKGVPYAQAERFQM
                     PTEVEPWEGIKETTSYGFVSPLLKQDTPSVELMIPHRYWPQDENCQNLNIWTKDLNRE
                     AKKPVLVWLHGGGYVAGSAIEHIAYDGFNMCDYGDVVVVTVNHRLNILGFLDLSFLGE
                     KYNNSANAGMADLVAALKWVNANIVEFGGDPDNVTIFGQSGGGGKVTGLMQIPEADGL
                     FKRGIVMSGVFDGKIMTQRKGGGERIVTAMMKELNISEDEAEKLETVPYYYLAEAYNK
                     VRPQIAEEGYYVGGSPLVNDYYLGEPLIKGHTEHAKTIPLMIGSVFGEFAFEKLPFNK
                     YELSEDEMEDILKEQYGQRAGEVIEQFKKSYPGKKIVDLLSIDRIFRAPSKALARAHA
                     GEGKAPAYLYNFTLDFPYQYGKPAWHCAEIPFFFHNTDKVEVCNIPGISDRLEKNIFN
                     AVINFARHGDPNHSGLPHWPNVTPEVEPTMIFDEECAVRNDYDDDLLALIDEILPPVK
                     LGI"
     gene            7342..7740
                     /locus_tag="VFD89_04770"
     CDS             7342..7740
                     /locus_tag="VFD89_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HZJ57536.1"
                     /translation="MPWSPSLSVGIELLDNQHKIWIERAEKLFDAGKKNQAKEYIGEM
                     LDFLEDYTKKHFKDEEKYMREIGYPGFEEQRKAHAVFIERLAKLQADYKESGGNISVI
                     VKANRIVVDWLINHITRMDKKIGEFVKENQ"
     gene            complement(7874..8086)
                     /locus_tag="VFD89_04775"
     CDS             complement(7874..8086)
                     /locus_tag="VFD89_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57537.1"
                     /translation="MNDISSMDLDGEIIRLTEELKSCQNSEKLLSISQDLDKAIRAYI
                     HFLKMNKGIKPTNRPGKSVSRTVRPH"
     gene            8233..8541
                     /locus_tag="VFD89_04780"
     CDS             8233..8541
                     /locus_tag="VFD89_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZJ57538.1"
                     /translation="MYGEFLADRIAKLRAMKKVSAREMSLSIGQNENYINHIENGKSM
                     PSMQGFFYICEYFGITPKEFFDEGINNPYLINKVAEDLNFLDEGQIANIYEIIKGLKK
                     "
     gene            8823..9479
                     /locus_tag="VFD89_04785"
     CDS             8823..9479
                     /locus_tag="VFD89_04785"
                     /inference="COORDINATES: protein motif:HMM:NF027487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4867 family protein"
                     /protein_id="HZJ57539.1"
                     /translation="MKTFEQLKNKNPQVTWIHADDPKLSPYGRVLDIDTSAYLKVVEE
                     EFADLGTTRVVYNASSPEFEALEDQNREVSDKVYGQMPIQVGCCYGFNSAMNAMEWHH
                     GSEVNVMATDTVLLLATFDKVDQGDGKLTFDSKDSVAVFVPKGYAIEVYSMVLHFAPI
                     QVDENGFFVLVILPQDTNTPLKRENEDDYLVAVNKWLIAHPDLGRRNDITGPNIKIAY
                     "
     gene            9782..10093
                     /locus_tag="VFD89_04790"
     CDS             9782..10093
                     /locus_tag="VFD89_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57540.1"
                     /translation="MKKYWITQDSKPCMTITLSSDATLQERYAAGELKNYLDYITSSS
                     MPIGDEEAHGSVIAIGKAAARIGVIAKDELGDDGFTLRTVGNSLAVVGGKRGIMRKHR
                     S"
     gene            10158..10613
                     /locus_tag="VFD89_04795"
     CDS             10158..10613
                     /locus_tag="VFD89_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57541.1"
                     /translation="MHEFLEYYYGAAAPFIRQYIRTLTDKVEGDNIHIGFNDQCDRPH
                     LTDDMLDIYDRLFDQAERAVMGDPIRAVRVDRARLSIRWVRLKNNAMLKGKKDPSKIN
                     QFFKDWHAHGLTRIDEWVSKETTHRALIEGLWRGTDFYKNWWEEGGEEL"
     gene            10827..11522
                     /locus_tag="VFD89_04800"
     CDS             10827..11522
                     /locus_tag="VFD89_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZJ57542.1"
                     /translation="MKKGRFGISLAAIAAIAFGFTALRQPQSVLLVAGFALLAEKDEW
                     LNRQVLQALLLTIAYYITSMVMDWIFGGIGRLFGFLGAYAAHTAMLSVNAFVGDVVNL
                     ALIVFSILAILKVLRGNDAGLPLVSKMAEGGLASTFSGRPGPGVPPTQQYQPVNGGNA
                     QPSGPGFTSHAPVQPGPSPTGGPDMQQVTAKTQGQIAEKEASGSPIKKCSSCGAALKP
                     ESRFCTQCGTKNE"
     gene            11549..12097
                     /locus_tag="VFD89_04805"
     CDS             11549..12097
                     /locus_tag="VFD89_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HZJ57543.1"
                     /translation="MFCENCGSSLQDGAKFCGSCGAKTEPIAPADETIQASTPEMSHQ
                     PQVAADNGAQPSAAKEAETPSYTPPARPAPAYTAPQPAQSYSTGQSYQPPAPNPYAPG
                     GFVDNSPLGIGQYIGMFLLMAIPLVNLILLFVWGFGSTVNVNKKNFARASLILMAISI
                     VLWIIIGSVIIGIIQSLTRGFY"
     gene            12195..13337
                     /locus_tag="VFD89_04810"
     CDS             12195..13337
                     /locus_tag="VFD89_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZJ57544.1"
                     /translation="MLFGGPGSGFSPKYMGCVNHTGDTVVPKSEDDNGLSYCLTRVDG
                     LNYISWRGVYKMLKINNLSSVGIITNYNCSSKCKHCSYASSPEWPKDYMTSDAADEIF
                     SILKILGCDHVHIGGGEPLLRPDRIFPILESAARNRIAIDYIETNASWYKDESSAMDV
                     LKEIMNRGVHQLLISIDPYHNEYIPFYKIKSLMTACRKANMGVFPWLMEFWDDLDSFD
                     DTRPHSLEEYGKLFGGGYLRRLPKRYGIGYKGRALKTYKDTFKTESFDSIMKKSRGCS
                     LLSGVYHSHVDLYGNYVPQSCPGLSIKLGELVDGADPEKYPIFHRLNTEGIRGLVELA
                     MDKYGYRPKREYAGQCDLCYDVRNYLVLTKGLDLPDLQPTGHYRFV"
     gene            13455..14783
                     /locus_tag="VFD89_04815"
     CDS             13455..14783
                     /locus_tag="VFD89_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020619194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HZJ57545.1"
                     /translation="MKTDELQLLRVSESKRFLTKEDGTPFFWLGDTAWELFHKLNRED
                     ADHYLKVRSQQGFNVVQAVALSEFDGIRTGNIYGRKPLLKNGEGIYDPSLPHVSSDPD
                     DYDYWDHVDFIINKAAEYGLYIALLPTWGDKFNQMWGKGPVIFNGKNAYDYGLWLGNR
                     YRDYNNIIWVLGGDRPLVTSRHFDVINAMAKGLKEGDGGRHIMTFHPVGGHSSSHHVH
                     EEEWLDFNMIQSSHGKVNNENYKMVAEDYNKMPVKPTFDAEPCYEDHPIGFNAVNGYF
                     DAADVRKAGYWSVFSGAMGHTYGHHCIWSMTTRVEAYFIMDWKTALLRPGAEQMGHIK
                     SLMESKPFTDRIPDQSIIAENYEGANHLRAIRGNNYAFMYSPGGLNTKVHMGIISGDP
                     VDAKWYNPRTGKYDSIGKFENKGTVDFLPPTAGRGEDWILVITDEDCPRH"
     gene            complement(14794..15471)
                     /locus_tag="VFD89_04820"
     CDS             complement(14794..15471)
                     /locus_tag="VFD89_04820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZJ57546.1"
                     /translation="MSIDNICIFGDSIGKGVVLNPDTKRYSVMKVDWESLLGGIGINI
                     NNYARFGCTVSKALSVIKRYTHELMGYSRVLLEMGGNDCDFIWKDVAENPDKEHSPRT
                     PPKEFRRLYGQIIDEIQASGGKPTVLNLPPLDPKRYFNWISRGLNADNILKWLGDVDM
                     IYRWQEMYNVEVMLLATRMGVPIIDIRSAFLKYNNYRDFLCYDGIHPNKKGYELIYRT
                     IAEQILP"
     gene            complement(15504..16295)
                     /locus_tag="VFD89_04825"
     CDS             complement(15504..16295)
                     /locus_tag="VFD89_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57547.1"
                     /translation="MKARLEKYINRKFLLYPKTRDIVEVRDELYSIVLDRYNDCLNLG
                     MSEGASYKMAIEIMADYREALKEVEKGSSLGALKRNLIGLIFFSSFYFITLTLIYLFV
                     SMVVLKTFEKTWLIAVGGSFIYLVYFSASTYGYARLFNFKRLERWGVALIYAGLIPIF
                     YVFPSLYLSAVHSKSIWEHSWIIALILVFLYIMTDYLLNRKNISTFQRQVQLLTAGLM
                     LTTISYLLVSIRFGLWDVAWILYVVYLAAASLTFYVRRKWGKFDE"
     gene            complement(16292..16630)
                     /locus_tag="VFD89_04830"
     CDS             complement(16292..16630)
                     /locus_tag="VFD89_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZJ57548.1"
                     /translation="MRYSSEILRGNTETIILAILSKKDSYGYEIMKTIIEGGEGLFSI
                     KDATIYTAFKRMEKNKLISSYWGDEDGSARRKYYTITQEGRDEYRRKVDEWEKINIVL
                     NKLITGGGLS"
     gene            17020..17538
                     /locus_tag="VFD89_04835"
     CDS             17020..17538
                     /locus_tag="VFD89_04835"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HZJ57549.1"
                     /translation="MLMGVEMVNIVLIGMPGSGKSTIGVLLAKALGMDFIDTDLLIQG
                     RMGTLLQDIIRDRGIGGFIHIENEIITGINCLNTVIATGGSAVYSHEAMGHLGSRGII
                     VYLEVGYNEIVRRITNITSRGIVFEEGEELIDLYDRRLPLYERYADLTIHCNHRDIES
                     IVSQIKEDVKGL"
     gene            17654..18922
                     /locus_tag="VFD89_04840"
     CDS             17654..18922
                     /locus_tag="VFD89_04840"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZJ57550.1"
                     /translation="MRNTFDEIFEYVKELDIIDSHEHLPHREDAREKDTDVIIEYLAH
                     YFNRDLISAGLSSEDFRRVMDPGMPIMEKWDIVEPFWNYSRYTGYGRALDISVKAIYD
                     IDGISGETIGDLNDAFKGSLKPGHFKKVLKDMSKIEVSLLDANLECDPYFFKSVYRLD
                     DFIYPKSHRDVAEIERKSGLRINSFDSWLEACETVLNKALKSGAAALKSGLAYDRSLR
                     YERVTRYDAELGFNEIFKTLNSPDWGDNPFNVGKPFQDYMMHFILGLANKGNLTFQFH
                     TGLQEGNGNLIYNSDPSLLSNLFLEYPDVDFDIFHIGYPYQHVLSALAKNFPNVYIDM
                     CWAHIISPAACVNALIEWIDSVPYNKITAFGGDYLIVDAVYGHQYLARENVSKSLAHK
                     VGEGIFPIDEAKHIAKALFYDNPKRIFKLN"
     gene            18973..20022
                     /locus_tag="VFD89_04845"
     CDS             18973..20022
                     /locus_tag="VFD89_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ57551.1"
                     /translation="MGDKKINIGIIGAGNIAQNSHIPAYLKQDDAKIIAICDINLDRA
                     KEVAAKHNIEYAVSSIEELVAIDEINAISICAWNNAHARAAIAAANAGKHVLCEKPMA
                     MTVAEAEEMKEVAEKNNIVFMMGFTNRFRADSKVIKEIADSGKLGDIYYARTGWFRRR
                     GTPLGWFTDLSKSGGGPVIDIGVHVIDLTWHFMGKPRPISVTATTYDRIGDYKTKGVS
                     RWEALDTDNPVFDTEDSAGGFIKFENGASMSFDVSWAINGKDTGIFSNIFGTKAGASL
                     NPFMIYGEEEGYLMDSSPSVESQNSFENEIRHFLDCVKTGEEPISSARDGVMIQKILN
                     GIYDSARLGKEVILD"
     gene            complement(20168..21601)
                     /gene="cls"
                     /locus_tag="VFD89_04850"
     CDS             complement(20168..21601)
                     /gene="cls"
                     /locus_tag="VFD89_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HZJ57552.1"
                     /translation="MNFYFSAIIFIINIALALTLIFLERRNPTTTWAWVLILMFVPGL
                     GFVLYLFLGQNYRKRKRITAKGHNDMIRVARQRSEMLTQHHLDFNDPNIFNIGDLILL
                     FYRNSGSVFSRNNKVTILNNGDEKFPALIEELRGAKHHIHMAYYIIRDDEIGRAVRDL
                     LAQKAQEGVEVRLLYDGMGCLFVGKRYFDPIIRAGGKVAAFLPPFIPYINVRVNYRNH
                     RKIIVIDGEIAFTGGLNLGDEYLGRSLKYGFWRDMHLMIRGSAVDQLQLRFMMDWQFA
                     SKKDIPASRKYFPHKYDTDGSGLQIVSSGPDSSWAAIHQGYVKMISRARKKVYIQTPY
                     FVPDDSMATALKIAALGGVDVRIMIPAMPDHPFVYWASTSYIGELLEAGAKAYIYDNG
                     FLHSKLLITDDSVASVGTANLDIRSFSMNFEVNTFIYDTDIIKKLEGFFLEDLRLCEQ
                     ITPESYAARPLSMRFKESVSRLLSPLL"
     gene            21798..22388
                     /locus_tag="VFD89_04855"
     CDS             21798..22388
                     /locus_tag="VFD89_04855"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZJ57553.1"
                     /translation="MDHYYSENPRVQHDIGRIEYSLGNIELSFITDAGVFSKDRVDYG
                     THILLRSLPPLVGKILDLGCGYGPIGITLARLNQNSEITMVDINQRAVDLAKINVEQN
                     GITNAGVLKSDGFENLDESFNAIISNPPIRTGKKVIYSLFEESGKYLQDGGSIYLVIQ
                     KKQGAKSAMEKLDQIYGNCSAINKKGGYWVLHSVKV"
     gene            22666..22911
                     /locus_tag="VFD89_04860"
     CDS             22666..22911
                     /locus_tag="VFD89_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HZJ57554.1"
                     /translation="MQEKDMVNDILSTLKSSIAGYAHIISEAGNEEFRQTVQQMRNGD
                     EKCQYDLFKIAQEKGYYIPAQAASPNDLQNVKSQFSG"
     gene            23061..23276
                     /locus_tag="VFD89_04865"
     CDS             23061..23276
                     /locus_tag="VFD89_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57555.1"
                     /translation="MAGMMEALGGMFCGKGGDQSLLFFFLLLVIIFCNCGLFNDGDEL
                     LFFFLLLILLFGGGSFFRGFGVQSKDS"
     gene            23685..25466
                     /locus_tag="VFD89_04870"
     CDS             23685..25466
                     /locus_tag="VFD89_04870"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HZJ57556.1"
                     /translation="MTGITDRIQGLREGMLDRGIDAYIVPSSDPHMSEYVAAHWEGRK
                     WLSGFTGSAGTLVITRDFSGLWTDGRYYVQAEKQLQGTGIQLFKAGMEGVKSFTQWIA
                     DTLDKGSCVGVDGKLFSLSQVRKMESRFSKKGISINKEHDLLMGIWKDRPPIPNGPVF
                     VHDIAYAGRTAAQKIEDVRARMTQKGTDYHLVSSLDDIAWLYNIRGSDVDYNPVAISY
                     ALVSDERAWLFINGEKVSEPVREHLKDNGIEVEDYEEIESHLSRLGEGDTILLDVNRV
                     NSWLYNAIPDGVGIEEDTTITTTLKAIKNETEIENLKRAHVRDGVAMVRFLYWLDNNI
                     SKGDITEITVADKLEEYRSQQENYVGLSFPTIAGYEDHGAIVHYQADEESAYTLGTDG
                     MILIDSGCQYLDGTTDITRTIPLGATTSEERRDYTLTLKGNIKLTRTRFLKGATGANL
                     DILARLHLWEHGIDYKHGTGHGVGYFLNVHEGPQGISPISSRVRLEAGMLVTNEPGVY
                     REGSHGIRIENELLVVEDIETEFGEFLRFEPITLCPIDLAPIELDLLDEGEKRWLNEY
                     HKWVYDILSPLLNQEESLWLKDKTRAI"
     gene            25647..26483
                     /locus_tag="VFD89_04875"
     CDS             25647..26483
                     /locus_tag="VFD89_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZJ57557.1"
                     /translation="MSMKIVADSCCDVNPEIEERTDIQLVPLTIQIEDKDYRDDKDLD
                     IDNLIVDMRNSPKVPKTACPSPQDFIERYKGGESVFVVTLSSALSGTYKSAMLAKEIF
                     LEEVENKFIHVFDSLSASVGETLISLHISELINKGLKEVEIVDKVNAYIKDMRTFFTL
                     KTLDNLVKSGRVSQFVARVSSILSVRPIMAGNRKGEIEVHEKIVGARRVFKRLVDVIG
                     EHGQNLEDKILGIAHCNALENALAFKEEVLKRYNFKDIIIVKTAGISTIYANEGGLII
                     AF"
     gene            26627..27598
                     /locus_tag="VFD89_04880"
     CDS             26627..27598
                     /locus_tag="VFD89_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HZJ57558.1"
                     /translation="MKGLGSMGVIPYILFIIGCIFIIKGGDLFVEAAIWIARVTGMSE
                     VLIGATVVSVATTLPELTVSTLSNLQGQASMAIGNAVGSTICNIGLILAVSCIIRPSR
                     IHSRFFHLKGIVLISYTLLLWGLSFKGGRIGTRDGWILLVLLASYLAIDYGIVKYKSP
                     NRAPSLLMPMAPKEWRGQFLRFLVGLVFILVGARLLVKYGIEIARFWHVPETIIALTI
                     IAIGTSLPELVTSMTAAIKGHEDLSVGNILGANILNITLVLGVSALVGPLEVPAQSNA
                     FDIPVSLVMGLILVVPGVVTRTISRLQGVVLLIGYLAYIAILFFVFG"
BASE COUNT         7980 a         5715 c         6896 g         7145 t
ORIGIN      
        1 gaggaaagaa tgatgaaagc tgaggttaac aggataacag aacacaagga cacaaatgaa
       61 atggatataa aagctactat cgatgcttta gttggaaaag gtaaccgggc tctatcccaa
      121 tttatgacca tggagcaaga acaggtggat cgcatcgtca aggcaatggc tttggccggc
      181 ttggatcagc atatgaagct ggctaagatg gctatagagg agacaaaaag aggtgtttat
      241 gaggataaga ttataaagaa tctttttgcc acggagtata tctaccatag tataaagtat
      301 gaaaggaccg taggcattat cgacgaaaat gacctggaag gctatgtgga ggttgcagag
      361 cccgtaggtg taatagcggg ggtcaccccg gtgaccaacc cgacttctac caccatgttt
      421 aaggctctta tatccataaa gacccgaaac cccattatat ttgcattcca cccatcagcc
      481 caagactgca gcagccgggc agccaaggtg atgctggaag cggccatagc tgaaggtgca
      541 ccgagagatt gtatccaatg ggtggaaacc ccatctttag aggcgacccg gggattgatg
      601 aaccaccccg gcatatccct tattctagca acagggggtt cgggaatggt gaaatccgca
      661 tatagcacgg gcaaacccgc ccttggcgtc ggccccggga acgtgccttg ctatatagaa
      721 aagtctgccg agataaaaag ggccgtaacg gatctcatcc tatctaagag ctttgataac
      781 ggaatgatct gtgcatcgga gcaggccatc atattggata gagagattgc agacgaggca
      841 atggatttca tgaaggaaaa cagctgctat ttcctatacg gcgaagaact aataaaggta
      901 gaggacatgg ttatcaataa ggaaaagggt gcagtcaacc cggtaatagt tggtcagtcg
      961 gcttttgaga tagcaaaact ttctggggtg aaggtcccgg aggacaccaa gatcttggta
     1021 gctcccatag acggggtggg tccggacttt cctctatccc gtgaaaaact gagcccggtg
     1081 ttagccctat atatagtaga aggctatgaa gagggtataa aaagggctga ggagatggtg
     1141 gcctttggcg gcatgggtca ttccgcggta atccattccg aggacgagag ggtaatcgat
     1201 ctcttctcgg agcgtataaa ggcagggcgc ctcatagtca attccccgtc gacccacgga
     1261 gccatcgggg atatctataa taccaacatg ccgtccctta ccttaggatg tggatccttc
     1321 ggccaaaatt ccaccacatc caatgtatcg gcagtgaacc tcataaacaa aaagagggtg
     1381 gccaggagga gggtcaacat gcaatggttt aaaatccccc agagggttta ctttgaatcc
     1441 ggctctaccc aatacctaga gaagatgccg gatatcagca gggcttttat agtcacagac
     1501 ccctttatgg tggaattagg atatgtggat agagtactat actatctcag aaagcgaagg
     1561 gattatgtac actgtaagat attctcccag gtagagccgg atccgtctat agagaccgta
     1621 aagcgtggaa ccgccgagat ggatgaattt aagccggatg taatcatagc cctgggcggt
     1681 ggttcggcca tggatgccgc taaggccatg tggctgttct acgaatatcc ggagacagac
     1741 tttactgctc taaggcttaa gttcatggat atcagaaagc gaacctttaa gttcccaaaa
     1801 cttggacaaa agacccagat ggtggccata cccaccacat cggggacggg ctcggaggtt
     1861 acctccttta ccgtaataag cgataacgaa aagaatatga aataccccct ggcagactat
     1921 gagctaaccc ctgatgtggc cataatagat ccggattttg tgatgacggt gcccccaagg
     1981 atcaccgcgg atacagggat ggacgttctt acccatgcta tagaggccta tgtatccatt
     2041 atggcctcgg attataccga cgccatggct atgaaagcca tccagctggt atttgagtat
     2101 ttgcccaagg cctataggga tggttccgat aggttggcaa gggagaagat gcacaacgct
     2161 tcctgtatag cgggtatggc ttttaccaac gcctttctgg gcataaatca tagccttgcc
     2221 cataagctgg gaggggaatt taatataccc cacgggaggg ccaatgccat actgctcccc
     2281 tatgttatag aatacaacgc acaaaagcct actaaatttg catccttccc taaatacgga
     2341 tactatgtgg cggatgaaaa gtatgcggag atagcaaaat acctgggcct tcccgcatcc
     2401 aacaaggaag aaggggttgc aagtctggtg aaggccatca gggttcttat gaaggagctg
     2461 gacgtgcccc tgaccatagc gcagtgcggt atccccgaga atgccttcga agcaaaggtc
     2521 gaagagctgg ccgacaacgc ctttgaggac caatgcacca cggctaatcc aagaatgccc
     2581 ctagtcaaag aactggaaga gatctacaga agggcatata ccggctaaaa cacaagaaaa
     2641 catacaaacg ctcaaatgca aatgcacaag tgcacaagta cagatgctca aggggacggt
     2701 tcccctgtgt tcggacacag aggaaccgtc cccttgtgtt cggaggaacc gtccccttgt
     2761 gttttagtca taaggggttc aaaatattgt tattgcattt taggggtttt tctaataaac
     2821 tatacacaaa agggttcccc tttgtttggc aattatgacg ataactacat tctgacaaag
     2881 tcgggccttt ttttctatgg agttttcccc acttttaaat gaaagataat tcaggccgta
     2941 tgcttagtcc ctacagggcc gtaaccttag atcccgggac taaacggata gataaatcaa
     3001 caataaaaag agggtaaatc attataggaa acagcgtctt tattaattac agatagacta
     3061 ctccaactat aaaatttatc atctatagat aaattcagtt tgcgtattgt ttatctatat
     3121 gtaaggggtt agtagaatgc ggcttttaaa accacatagg aattggacat ttgcgattat
     3181 atactttttt gcatgggctg cttatgccac atttacgctt gacttcatct tctacagtca
     3241 gcagattgga tttaacaata tagaaatcgg tattctctat tctacgctgg ggcttatagg
     3301 tattatgtca cagccaatat tcgggtatat ctccgattac tttcagagca ctcgtaaggt
     3361 tctgatggga tcccttacct ttgggatagg gctcagtatg ctcttgccct ttgttaagga
     3421 caagatgaca gtccggatca tagccatggt ttacacggtt ttcatgagct ccttcatgcc
     3481 ccttttggac aattgggtag ccagttcgtg cataggagag cagaagggta attacggata
     3541 tataagacta tggggttccg ttggctatgc gataatggca tttgtctatg gcaaattgac
     3601 catgagtatt gacgtttcca acatctattt cagcaggggc gcattgtttg taatagtatt
     3661 tatttttatc tataccttta ggcatgagga tatgttagat aagaatgaag tgatgtcaga
     3721 taggcatgaa ggtatcttag aaagtgaaag gaagaaggat agacccaata tcaagggact
     3781 attctcaaac aaggaatata tactgtttat aatctttgct tttgtatttt cctttccctt
     3841 aaacgcggcg agtactttct ttcccagcct gctgctggaa atcgggggca ccaacgcaac
     3901 catcggattc gcgggctcta tcaatgctat tgtggagatc ccgtttttcc tctacaccca
     3961 aaagttgggg aaaagaatgg gccacaaggg tctaatgttt gtaggatgcg tatttatgtg
     4021 catgagaatg ttcgggttca tctttgccaa gagcattccg ggtttaatgg ttgcttattt
     4081 ctttatggct ccttacacca gctttttcgc accggggatg atatactata ttcattccat
     4141 atcgccccca aacacccagg ttgttgcaca aaccattatt caggcctttg ccatgggcag
     4201 tgcgggaatg ataggcaatt atctggcggg ttatttcatc gacagctatg gtatacgggc
     4261 tatgtataaa tatggaaccg tcatttgtat agctgcggta gtcttcttct ttttgtcggg
     4321 gtggataata ggacgtctta aagggggaca aattggcata gaagttcaga aaaagaatat
     4381 gattgccaat gaacatgagg tataatagaa agaagagaaa caatcgacgg attatttcaa
     4441 ataaaagagt ctaagactaa aatccgaaga aaggatggat agggtatgaa aaagctctat
     4501 ggttacatgg caggtaccaa ccttgggggg tggatatccc agtatcgaga actgaaccat
     4561 gaccacttca aatcatttat tgtggaagag gacattgctc ttatagcttc ttggggcatg
     4621 gatcatattc gggtccccat cgattatccc gtgcttgaag aggatgttaa tcccggggta
     4681 taccttgaaa gtgggtttca gtacctcgat aattgtatcg agtggtgtaa aaagcatggg
     4741 ctgaaccttg ttatcgatgt gcacagggcg cccgggttca gctttaatac gctggataag
     4801 aacacactat ttacagatcc aaaaatgcag cagcgctttg tagacctatg gatcgccatt
     4861 gctcgtagat atgcaggtga gagcgacaac ataatgtatg aacttctaaa cgaaatcgtg
     4921 gaacccgaca gtctgcgctg gaacgatctg gccaccagat tgatagaggg tatccgaaaa
     4981 gtagataaaa agcatagaat tgtcgtaggg ggcatcgact ataacagcgt aaatgccctt
     5041 aaggacatgc ccatatttga tgatcccaat atcgtatata actttcatat gtatgagccc
     5101 tttgcgctga cgcatcaacg ggccagctgg agcaaggagg ccatggaatt tgacaggaca
     5161 atgcgctacc cggatgagag cctgcaggac tatattgatt ttgccaattt cacgccttcc
     5221 cggggcctcg taaatcagta cgagggactg acaaggatgg atgttaacta catacggaag
     5281 tttttaaaac ccgccataga atttatagat aagcacgacc tcccgctata ctgcggtgaa
     5341 tatggggtga tcgaccatgc cgatatggcc agccgggaag catggagtga ggatttggca
     5401 gaggttttgc tggaatatgg tataggacgg gcggtatgga cctataagag tatgagcttc
     5461 gctcacatcg atggggatag aaatccgctg agccaacggc ttataagggc ggtcagcagg
     5521 cactagaaaa tgttcggcaa ttttatgaaa gcaagagaaa ggggaaagaa tttatgggta
     5581 tgatatttga atatgatgag gtaccaagtg tccaaacgaa atccggtaag ataaaaggat
     5641 atgcctataa cggggtacat atctttaagg gtgtccccta tgcccaggct gagagatttc
     5701 agatgcctac agaggtagag ccttgggaag ggatcaaaga aacaacctcc tacgggtttg
     5761 tcagtccgct tctaaagcag gacactcctt cggtggagct catgataccc cacaggtatt
     5821 ggccccaaga tgagaattgc caaaatctca atatctggac caaggattta aatagagagg
     5881 ctaaaaagcc ggtcctcgtt tggctccatg gcggaggcta cgtcgccgga tcagccattg
     5941 agcacatagc atacgacggg tttaatatgt gcgattatgg ggatgtagtg gtggtaaccg
     6001 taaatcatcg gctcaatatc ctgggctttt tagacctatc cttcttgggg gagaagtaca
     6061 ataactccgc aaatgcgggt atggcggacc tggtggcggc attgaaatgg gttaatgcca
     6121 atatcgttga gttcggcggg gatccggata acgtaaccat attcggacag tccgggggtg
     6181 gcggtaaggt cacagggctc atgcagattc ccgaagccga cgggctcttt aaaaggggaa
     6241 ttgtcatgag tggtgtattc gacggcaaga taatgaccca gcgaaaaggc ggcggagaaa
     6301 gaattgtcac cgccatgatg aaagagctga atatttccga agatgaagcc gaaaagctgg
     6361 aaaccgttcc gtattactat ctggcagagg cctataacaa ggttaggccc cagatagcag
     6421 aggaaggata ttatgtcgga ggttcgcccc tagtcaacga ttactacctc ggcgagccct
     6481 taattaaggg acacacggag cacgcgaaga cgatccccct catgataggc tcggtgtttg
     6541 gggaatttgc ctttgaaaag ctacccttta acaaatatga acttagcgaa gatgagatgg
     6601 aggatatact caaggaacag tatggccagc gcgcgggaga ggtcattgaa cagtttaaaa
     6661 aatcctatcc cggaaagaag atcgtagatc tgttgtccat tgacaggatt ttccgtgccc
     6721 cttccaaggc cttggcaaga gcccatgccg gagaaggcaa ggcgcctgcc tacctctaca
     6781 actttacgct ggattttccg tatcaatatg ggaaacccgc atggcattgt gccgagatac
     6841 ccttcttttt tcataacacg gacaaagtgg aggtatgcaa catcccgggg atttcggata
     6901 ggcttgaaaa gaatatattc aacgccgtca taaactttgc ccgccatggc gatcccaacc
     6961 atagcggatt gccccattgg cccaatgtta cgccggaagt tgagcccacc atgatcttcg
     7021 atgaggagtg tgcggtgcga aacgattatg atgatgatct gctggccttg attgacgaga
     7081 ttctgccccc tgttaagttg gggatataat aaaggggtgc ttttggatta acaaaggcag
     7141 aatccgacaa atagcacatc cgttatggct gaaatacgca ttaaagaaga aaaataatgt
     7201 cgaattgagt ttatagtgtt aatatattcc cgaaatggtt tattatatat gaaacatcca
     7261 aggtgctgat tagatatacc cctaaaagct tttatggggt atatccggca ccatgaaaat
     7321 tatatagggg aggtttttca tatgccatgg tctccatctt tatctgtagg gattgaacta
     7381 ctggacaacc agcataaaat atggattgaa agggcggaga aactctttga tgccgggaaa
     7441 aagaatcagg ccaaagaata cataggagaa atgcttgact ttctcgagga ttataccaaa
     7501 aagcacttta aggacgaaga gaagtatatg agggaaattg gataccccgg gttcgaagaa
     7561 caaagaaagg cccacgcagt ttttatcgag cggttggcca agctgcaggc ggattataag
     7621 gaatctggcg gcaatatttc cgtaatcgta aaagccaaca ggatcgtggt agattggctt
     7681 ataaaccata ttaccaggat ggacaagaag attggcgaat ttgttaagga aaatcaatag
     7741 gtgcccagcc aaagaaaatg ctctgcaaca agctcatagg cggatcttaa agatctgcct
     7801 attgttgtat gaaaaagatt tcttcaagcg cacaaatcaa tcggttaaaa tctttacatt
     7861 aaaaaagacg actctaatga gggcgaacag tccggctgac ggatttaccc ggtctgttcg
     7921 taggttttat gcccttattc attttcagaa aatgaatata tgcgcgtata gccttatcca
     7981 gatcctggct gatactaagc agcttttcac tattctgaca gctctttaac tcctcggtta
     8041 acctaattat ttccccatct aaatccatgc tagatatatc gttcaccccc caatctctgt
     8101 atttcacggt cagattgcat taaagacaag tatacaatat atgatgttgt atgtcaacta
     8161 aagtaatact ctatacattg cagatgtgtt gcaaggacaa ggtacaatat tgcacaaaag
     8221 gtggcgatat gcatgtatgg ggaattttta gctgatcgga tagccaaatt acgagccatg
     8281 aaaaaggttt ctgcccgtga aatgagctta tccatcggac aaaatgaaaa ctacataaat
     8341 cacatagaaa atggaaaatc aatgccttcg atgcagggtt ttttttatat atgtgaatac
     8401 tttgggataa ctccaaagga attttttgac gaggggatca acaatcccta cttaatcaat
     8461 aaggtagcgg aggatctgaa tttcctggat gaaggccaaa tagccaatat ctacgaaatt
     8521 attaagggac tgaaaaagta gatcggatag gtcctataag ccggagtcat gggctttttt
     8581 gtggtaccct aaatattttg gcattagggt gcttcatctg tgtagacaaa tatggtatat
     8641 tatatacgac acaataatat acaggcaatt aggttatatc ctatttgtct gtaatataga
     8701 tagataatac ggaagctaaa tttatctgta gatgatagat tttatagttg acgttgttta
     8761 tctataacat tatgcactac tatttgtaga aacattaacg cttaatgaga ggatatgaaa
     8821 gtatgaaaac atttgaacag ctgaagaaca aaaacccaca ggttacatgg atccatgcag
     8881 atgatcctaa gctctccccc tatggacggg tattggacat tgatacctcc gcctacctaa
     8941 aggtcgtgga agaagagttt gccgatttgg gaactactag ggtggtatac aatgcctcaa
     9001 gcccggaatt tgaagcgctg gaggatcaaa accgggaagt gagcgataag gtatatggac
     9061 agatgcctat ccaggtaggg tgctgctacg gatttaattc cgctatgaat gctatggaat
     9121 ggcaccacgg cagtgaggtc aacgttatgg caacggatac cgtactcctg ttggcaacct
     9181 ttgataaggt agaccaaggg gatgggaagc tcacatttga ttcaaaggac tccgtggccg
     9241 tttttgttcc caagggatat gccatagagg tctattcaat ggtgcttcat tttgccccta
     9301 tacaagtaga tgagaatggt ttctttgtcc tggtaatcct gccccaagat accaataccc
     9361 ccttaaagag agagaacgag gatgattacc tagtggcagt taacaagtgg ctaatcgctc
     9421 acccagattt gggccgcaga aatgatatta cgggacctaa cattaaaata gcatattaac
     9481 aagaataaat agaagttata gatacaatcc ggtgctgcaa atcggcgggc tggaacaccg
     9541 actgcactta tatatggtta taggtagaat gcgatctcgg gtagcaggcc tgagaccgca
     9601 ttttacaaaa ataccatgca tatataatca tatctatgta gtatatatgg actaggtagc
     9661 gggcatatga aattttttta atgattagga tgttggacta aatcaaccta ttgtcttagg
     9721 cacaatcaaa ccagggaaaa tataatcttg tcctgcctaa ttaagaaagg gagatggaaa
     9781 gatgaaaaaa tactggataa cccaagacag taagccctgc atgacaatta ccctatcatc
     9841 agatgctact ctgcaggaaa ggtatgccgc cggcgaatta aaaaattact tggattacat
     9901 aacatcttcg agcatgccta ttggggatga agaggctcat ggatctgtta tagctatcgg
     9961 taaggcagcg gcacgcattg gcgtcatagc gaaagacgaa ttgggtgacg atggatttac
    10021 gttgcggaca gtgggcaaca gtttagctgt cgtaggcggg aaaaggggta tcatgaggaa
    10081 gcaccgatct taatgagctt agggcctacc tgatagccaa gctgctttgg gatgcagact
    10141 gcgattacga aaggcatatg catgaattcc tagaatacta ttacggagca gccgcgccat
    10201 tcattcgtca atatataagg accctgactg ataaggtcga gggggataac atccatatag
    10261 gctttaacga tcaatgtgac cgtccacatc tgaccgatga tatgctagat atctatgata
    10321 gactgtttga tcaggcagaa agggcggtaa tgggggatcc tatccgggct gtgcgggtgg
    10381 atagggctcg gcttagtatt cgctgggttc gtcttaagaa caatgccatg ctcaagggta
    10441 aaaaggatcc atctaagatc aaccaattct ttaaggattg gcacgcccat ggattaaccc
    10501 gaatcgatga atgggtcagt aaggagacga cccatcgtgc attaatcgag ggattatggc
    10561 gaggaactga tttctacaag aattggtggg aagaaggcgg ggaagaattg taatccccat
    10621 cacaactgtc atatcaattg cttcgatata ttgatctctg ataccaaaga tttctgtaac
    10681 cgtgctaaaa agggggcgtt aacgcccccg taaccgcttt tatggcggat attttaattt
    10741 atgtgttata ttttatcaat atgtgttata tatatccaaa tatccgcttt tttaataaat
    10801 actgacaaaa gaaaggatgt tttaccatga aaaaaggaag gtttggaata agccttgccg
    10861 caatcgcagc gattgccttt gggttcaccg cattgcgcca accccaatcg gtattgctgg
    10921 tggcgggatt tgccttactg gcagaaaagg acgaatggct taatcgtcag gtattgcagg
    10981 cacttctatt gaccattgca tactatatta cctctatggt tatggattgg atctttggag
    11041 ggattggcag attgtttggg ttcttggggg catatgcggc ccacactgct atgctcagtg
    11101 ttaatgcctt cgtaggcgac gtagtcaatt tagcccttat tgtattctct attttagcga
    11161 ttttaaaggt tcttcgcggt aatgatgcag gcctgccact agtatcaaag atggcggaag
    11221 ggggtctcgc ctctactttt agcggacgac cggggccagg ggttccccca acgcaacaat
    11281 accaacccgt taatggggga aatgctcaac catccggccc cgggtttaca tcacacgcac
    11341 ctgtccagcc tggtccctcg cctacaggag ggcctgatat gcagcaggta acggcaaaga
    11401 cacaaggcca aattgcagaa aaggaagcat cggggagccc cataaaaaaa tgttcttctt
    11461 gcggtgcggc tttgaagccc gaatcccgat tctgtaccca atgcggtaca aagaacgaat
    11521 aattcaattt tgaaatggag gaaaagcaat gttttgtgaa aattgtggta gcagccttca
    11581 agacggagct aaattttgcg gcagctgcgg agcaaagacc gagcctatag cacctgcaga
    11641 tgagactata caggcatcca cgccggagat gtcccatcaa ccccaggttg cggcggataa
    11701 cggggcacaa ccatcagcgg caaaagaagc agagaccccg tcctatacgc cgccggcccg
    11761 gcccgcaccc gcttatacgg caccccaacc tgcccagagt tattctacag gacaatccta
    11821 ccaaccccca gctcctaatc cctatgcccc tgggggattt gttgataatt cgcccctagg
    11881 gatcggacaa tacataggta tgttcctttt gatggccata cctttggtaa atttgatttt
    11941 gttgtttgtc tggggattcg ggtcaacggt aaatgtgaat aagaaaaact tcgcaagggc
    12001 ctccctcatc ctgatggcca tttccatagt gctttggatc ataataggca gcgtaatcat
    12061 aggtattatc cagagcttaa ccaggggttt ttattaaaac gctaagggaa atcctaacaa
    12121 tcgggtctcc attagcttaa acttgttttt gttctgcacc gcctccgtac cgaaaatcgg
    12181 tattggaggc ggcgatgctt tttggcggcc caggatctgg cttttcccca aaatatatgg
    12241 gatgtgtaaa ccataccggg gataccgtcg taccaaaatc cgaagatgat aacgggcttt
    12301 cctattgcct tacccgggtt gatggtctaa actatatatc gtggaggggt gtttataaga
    12361 tgttaaagat caataattta agttcagtcg gaataatcac aaattacaat tgttcatcta
    12421 aatgtaagca ttgttcctat gccagttccc cggaatggcc aaaggactac atgacctcgg
    12481 atgcagccga tgagatattc tctatcttaa aaatacttgg ctgtgaccat gttcacatag
    12541 ggggcggtga acccctattg cgcccggaca ggatatttcc tatattggag tccgctgcaa
    12601 gaaacaggat cgccatcgat tatattgaaa ccaacgcatc ctggtataag gatgaatcat
    12661 cggctatgga tgttctaaag gagataatga atcgcggtgt acaccagctt ttaatctcca
    12721 tagatccgta tcataacgag tatatacctt tttacaagat taagagcctg atgacggcat
    12781 gcagaaaggc caatatgggc gtgttcccat ggctgatgga attttgggat gacctggatt
    12841 cctttgatga cacaagaccc cattccctag aggaatatgg aaaactcttc ggtgggggtt
    12901 acctgaggcg cttgcccaaa agatatggga ttggctataa gggtagggcg ttaaagacct
    12961 acaaggatac atttaagacg gaatcctttg acagcataat gaaaaaatcc aggggatgca
    13021 gcctactatc gggggtctat cattctcacg tagaccttta tggaaattac gtcccccaat
    13081 catgtccggg tctttctatt aagttagggg aattagtgga cggggcggac cctgaaaaat
    13141 atcccatatt ccataggcta aataccgagg gtataagggg attggtggaa ctggccatgg
    13201 ataaatacgg ttatagacca aaacgtgaat atgcgggaca atgcgatctc tgctacgatg
    13261 tccgcaatta cctggtactg acaaagggat tggaccttcc ggaccttcaa ccgacgggcc
    13321 attacaggtt tgtttaggtg atggaaaggt tttcaggtta taatgaactg atgcaacaga
    13381 cagattcaac ttttgtataa ctctaattta ctagttacct aatcatacca aaaagtttaa
    13441 ggcaggtgtt atttttgaag accgatgaat tacagctatt aagagtcagc gagagcaaaa
    13501 ggttccttac caaggaggac ggtacaccgt ttttttggct gggggacacc gcatgggagc
    13561 tgtttcacaa gctaaacagg gaggatgcag accattattt aaaggtgcga tcacagcagg
    13621 ggtttaacgt agtacaggct gtcgccctat cggagtttga cggtatcagg accggaaata
    13681 tctatgggag aaaacccctg ctaaaaaatg gggaagggat atacgacccc agtcttcccc
    13741 atgtttcatc tgatcctgat gattatgact attgggacca tgttgatttt atcatcaaca
    13801 aagccgcgga atacggcctc tacattgccc ttcttccgac ctggggtgat aaattcaacc
    13861 agatgtgggg aaagggtccg gtaatattca acgggaaaaa cgcctacgac tatgggctgt
    13921 ggttgggtaa tcggtacagg gattacaaca atattatttg ggtgttagga ggcgacaggc
    13981 cccttgtgac ctcccgccat tttgatgtta taaatgccat ggcaaagggc ctaaaggagg
    14041 gagacggcgg caggcacatt atgaccttcc atcccgttgg tgggcattcg tcctcccatc
    14101 atgtccatga agaggagtgg ttggacttta atatgatcca gtccagccac ggaaaagtga
    14161 ataatgaaaa ctataagatg gtagccgaag attacaataa aatgcctgta aagcccacct
    14221 ttgatgcgga gccgtgctac gaggatcatc ccatcggatt taatgccgtg aacggatact
    14281 ttgatgccgc cgatgtaaga aaggccggat attggtcggt tttttcaggt gccatgggcc
    14341 atacctacgg gcaccactgc atatggtcca tgaccacacg ggttgaggcc tattttataa
    14401 tggattggaa gaccgcactt ttacggccgg gtgcagagca gatgggacac ataaaatctc
    14461 tgatggaatc gaaacccttt accgatagga tacccgacca atccataatc gccgaaaact
    14521 acgaaggggc aaatcacctg agggccataa ggggcaacaa ctatgctttt atgtattccc
    14581 ccggcggcct gaatactaaa gtacatatgg gcattatatc gggggatcct gtagatgcca
    14641 agtggtataa cccccgaacc gggaaatatg acagcatagg taaatttgaa aacaagggta
    14701 cagttgactt tctcccaccc actgctggca ggggagagga ttggatcttg gtgataaccg
    14761 acgaagactg cccccgacat tgatttggga aaactagggg agaatttgct ccgcgatagt
    14821 cctgtagatg agctcatagc cctttttatt gggatggatt ccgtcatagc acagaaaatc
    14881 ccggtagtta ttatatttta aaaatgcgct gcggatatcg ataatgggca ctcccatcct
    14941 ggtggccagg agcatgactt cgacattgta catctcctgc cagcgataga tcatatctac
    15001 atcccccagc cattttagga tgttgtcagc gtttaaccct ctggaaatcc agttaaagta
    15061 tcgtttgggg tctagtgggg gaaggttaag gaccgtcggt tttcccccgc tggcctgaat
    15121 ttcgtcaata atctgtccat atagtctcct gaattccttg gggggagtcc ttggggaatg
    15181 ctccttgtca ggattttccg ctacatcctt ccatataaaa tcgcaatcgt tgccgcccat
    15241 ctcaagaagg accctactat atcccataag ctcatgggtg tatcgtttta tgaccgacag
    15301 ggcctttgaa acggtacaac caaacctggc ataattattg atgttaatgc ctattccgcc
    15361 gagaagggat tcccaatcca ccttcattac gctatagcgt ttggtatctg gatttagcac
    15421 aacacctttc ccgattgaat ctccaaatat acagatatta tcgatagaca tatccagcct
    15481 cctataatct attggtccgt gttctattca tcaaactttc cccatttgcg cctcacataa
    15541 aaggtcaaag aagccgccgc caggtatact acatacaaga tccatgcaac atcccaaagt
    15601 ccgaatcgta ttgatacaag cagatacgag atggtggtaa gcatcagtcc cgctgtaagg
    15661 agttgcacct gcctctgaaa cgtggatata ttcttccggt taagcaagta gtctgtcata
    15721 atatacagaa atacaaggat caaggctatt atccagctat gctcccatat gctcttagaa
    15781 tgaactgcgg ataggtacag ggatgggaaa acgtagaata tcggtatcaa gccggcatag
    15841 ataagcgcca ctccccaccg ctcaagccgc ttgaagttaa atagtctggc atagccatat
    15901 gtacttgctg aaaaatatac caggtatata aaggagccgc cgacagcaat caaccaggtc
    15961 ttttcaaagg tttttagtac aaccatgctt acaaagagat agatcagggt cagggtaata
    16021 aagtagaatg agctgaaaaa gataagcccg attaaatttc tcttgagggc tcctaaggag
    16081 ctcccttttt ctacctcttt taaggcttcc ctgtaatctg ccattatctc tatggccatc
    16141 ttgtagcttg cgccctcgct catccccaag ttcaagcaat cgttatacct gtcaaggacg
    16201 atggaatata gttcatcccg tacttcgacg atatccctgg ttttggggta caaaaggaat
    16261 tttctgttta tgtacttttc aagtctcgcc ttcacgataa gccacccccg gtgatcagtt
    16321 tgtttaagac gatgtttatt ttctcccact catcaacctt tcgcctatac tcatccctgc
    16381 cttcttgtgt gatggtatag tacttgcgtc tcgcactgcc gtcctcgtct ccccagtagc
    16441 ttgaaatcag cttattcttt tccatgcgct taaaggccgt atatatcgta gcatccttta
    16501 tgctgaacaa accctcaccc ccctcgatga tggtcttcat gatctcatag ccgtatgaat
    16561 ccttttttga taaaatggcc agaataatgg tctccgtatt gcctctcagt atctcgctgg
    16621 aatagcgcac cttatctccc ccgatatcta acctggttag gtatattgta acgttattat
    16681 gaatgactgt caaccatacc tcaaaaataa tttagcttaa aatcttagcg tatttaatac
    16741 gggggcatga aaggtattga gaaaatgggg ggatccgatg tataatggat aaggcggggg
    16801 agacggaagg agccgtcggt aaaaattatg gaggcgggtc atccggtaca tacaaggttt
    16861 taaaacatcg gaagcaattg cattggcaat tgcaacacac aggtttaaac gaggcggggg
    16921 atttatcccc accacatgtt ttgctaatgg gaactcgccg cttatagaag cgggagacat
    16981 cttaccgact tgttttaatt tggcataaga gggataggaa tgttaatggg cgttgagatg
    17041 gtaaacatag tcctaatagg gatgccgggg tcaggtaaga gcacaatcgg cgtcttgttg
    17101 gcgaaggcct tgggaatgga ttttatcgat acggatttgt taattcaagg aaggatgggt
    17161 accctgctgc aggacatcat cagggatagg ggtattggtg gatttataca catagaaaat
    17221 gaaatcataa ccgggataaa ttgtttgaat accgttatcg ctaccggtgg gagtgcagta
    17281 tacagccatg aggctatggg gcatttaggg tcaaggggaa tcatagtcta tttagaggtc
    17341 ggttataatg agatagtcag gcggattacc aacataactt ccaggggcat tgtattcgaa
    17401 gagggggagg agctgattga tctatatgat agaaggctac ccctatatga aaggtatgct
    17461 gatttaacaa tccattgtaa ccatagggat attgaaagta tagtcagcca gataaaagaa
    17521 gatgtgaaag gcttataaaa ttcctaccat cagcaggttt ttatgttatc tatatgttct
    17581 acaataaaca ttctacattc accatatttg aactacatgg ccaaaatatt ttaaaaagct
    17641 gggaggaagg tctatgcgta atacgtttga tgagattttt gaatacgtta aggaactgga
    17701 cataatcgat tcccacgagc atctgccgca tcgggaagat gccagggaaa aggataccga
    17761 cgttattata gagtatctgg cccactactt taatagggat cttatatcgg cgggactcag
    17821 ttccgaggat tttcgcaggg ttatggatcc cggtatgccc attatggaaa agtgggacat
    17881 agtcgaacct ttttggaatt attcccgcta taccggatac ggaagggctt tggatatatc
    17941 ggttaaggct atctacgata tagatgggat ttcaggggag acaattgggg atctcaacga
    18001 tgcctttaaa ggatccttaa agccgggaca ctttaaaaag gtattaaagg atatgtcaaa
    18061 aatcgaggta agccttttag acgcaaacct tgagtgtgat ccgtactttt ttaaaagcgt
    18121 ctatagactg gatgacttta tatatcctaa aagtcacagg gatgtagcgg agatagagag
    18181 aaaatccggg cttagaataa attcttttga cagttggctt gaggcatgcg aaacggtatt
    18241 aaataaggct cttaaatccg gcgccgcagc cctgaaaagc ggactggcct acgaccgtag
    18301 cctaaggtac gaaagggtaa caaggtacga tgcagagctg ggctttaatg agatcttcaa
    18361 aaccctaaat tccccggatt ggggggacaa tccctttaat gtaggaaaac cgtttcagga
    18421 ttatatgatg cactttatct tgggactggc caacaaaggg aatcttacct tccagtttca
    18481 tacaggcctt caggagggca acggaaacct tatctataac agcgatcctt cgctgttatc
    18541 caacctgttc cttgaatacc cggatgtgga ctttgatatc tttcatatag ggtatccgta
    18601 ccagcatgta ttgtcggccc tggcgaagaa tttccccaac gtatacatcg atatgtgctg
    18661 ggcccatatt atatcacctg ccgcatgcgt taatgccctg atagaatgga tcgattccgt
    18721 tccgtacaat aaaataactg catttggcgg ggattacctg atcgtagacg ccgtatatgg
    18781 gcaccaatat ttggccaggg aaaacgttag caaatcctta gcccacaagg ttggggaggg
    18841 gatattcccc atagacgagg ccaaacatat agccaaggct ttgttctatg ataatccaaa
    18901 gaggatattt aagctgaatt aacctaagtt aagccagctg aatatagact aacaatcaag
    18961 gaggaaacaa ccatgggaga caaaaaaatt aatatcggaa ttattggggc aggcaacata
    19021 gcccagaact cgcacatacc cgcctatctc aagcaagatg atgccaagat aatcgccata
    19081 tgcgatataa accttgatag ggccaaggaa gtggcagcca agcacaacat tgaatatgcg
    19141 gtgagttcca tagaagagtt ggtggccatc gatgaaatta atgctatcag tatctgcgct
    19201 tggaacaatg cccacgctag ggccgctatt gcggctgcca acgcagggaa gcacgtcctg
    19261 tgcgaaaagc ctatggccat gaccgtagcc gaggcggagg aaatgaaaga ggttgcagaa
    19321 aagaataaca tagtatttat gatgggcttt accaaccgtt ttcgtgccga tagcaaggtc
    19381 atcaaggaga tagcggacag cggtaagctg ggcgacatct actacgcaag aaccggttgg
    19441 ttccgcagaa ggggaacacc ccttggctgg tttaccgacc tttccaagtc cggcggcggc
    19501 cccgttatag acatcggtgt ccatgtaata gatctgacct ggcactttat gggcaaacct
    19561 cgtcccataa gcgtaacggc cacaacctat gacaggatcg gcgactataa gaccaaaggg
    19621 gtcagtcgct gggaagctct ggatacggat aacccggtat ttgataccga ggattccgcc
    19681 ggcggtttta taaaatttga aaacggcgca tccatgagct ttgacgtaag ctgggccatc
    19741 aacggcaagg atacggggat cttcagcaac atctttggaa ccaaggccgg tgcaagcctc
    19801 aatcccttta tgatatacgg tgaagaggaa ggatacctaa tggattcctc ccccagtgta
    19861 gaatctcaaa atagcttcga aaatgagatc agacactttc tagactgcgt caaaaccggg
    19921 gaagagccta tctcgtctgc aagggacggg gtcatgattc aaaagatatt aaacggtatc
    19981 tatgattctg caaggcttgg gaaagaggtt atattggatt aaaatctgct tacaccaatc
    20041 cattttccat agttgtatgt tttacgataa acttaccata ctaaagataa acaattgttg
    20101 attgaataaa ttcgttataa ataggtgttc gaacccggat aggccgctga ggcctgcccg
    20161 ggttttttca aagcaggggt gagaggagcc tggaaacgga ctccttgaat ctcatggaaa
    20221 ggggacgggc cgcatagctc tccggggtga tttgctcaca gagtctcaaa tcttccaaga
    20281 agaatccctc cagcttttta ataatatctg tgtcgtatat aaaggtattg acctcaaagt
    20341 tcatagaaaa gcttcgaata tccaggttag cggtgcctac ggaggcgacg gaatcgtcgg
    20401 taataaggag cttgctgtgt aaaaaaccgt tgtcgtagat ataggccttt gcgccggcct
    20461 ccaacagttc gccgatatac gacgtagagg cccaatagac gaagggatgg tccggcatgg
    20521 cgggtatcat gatcctcaca tctaccccgc caagggcagc tatcttcagc gccgttgcca
    20581 tgctatcatc gggaacaaaa tagggtgttt ggatatagac cttcttccga gccctcgaga
    20641 tcatcttgac atagccctgg tgtatggctg cccaggatga atcgggtccg ctggaaacta
    20701 tctgcaggcc ggagccatct gtatcatact tgtggggaaa gtatttgcga gaggcaggta
    20761 tatccttttt ggaggcaaat tgccagtcca tcataaagcg aagctgcagc tggtctaccg
    20821 cactgcctct tatcatgaga tgcatgtccc tccagaatcc gtactttaag gatctaccca
    20881 ggtattcgtc ccccaggttc aggccgccgg taaaagcgat ttcgccgtct atgactatga
    20941 tcttcctatg atttctgtag ttcacccgga cgtttatata tgggatgaag ggtggcagga
    21001 aggccgcaac ctttccccct gcccggatta tggggtcgaa gtaccttttt ccgacaaata
    21061 ggcatcccat cccgtcgtag aggagcctca cctctacgcc ttcctgggcc ttttgagcca
    21121 gcaggtcccg gaccgccctt ccaatctcat cgtctcgaat tatgtagtag gccatatgta
    21181 tgtggtgctt tgctcccctc agctcttcta tgagggccgg aaacttttcg tccccgttat
    21241 ttaggatggt caccttattg tttctggaga atacggaacc ggaatttcgg taaaacaaaa
    21301 ggatcagatc ccctatattg aaaatattcg gatcattaaa atccaggtga tgttgggtca
    21361 gcatctctga cctttgccgg gctactctta tcatatcgtt atgccctttt gcggtaattc
    21421 tttttctctt tctgtagttc tggcccagaa acaggtagag aacaaagccc agccccggaa
    21481 cgaacattag aattaggacc caggcccatg ttgtggtggg attgcgtcgc tccagaaata
    21541 taagggtgag ggcaagggca atatttatga taaatataat agcagaaaag taaaagttca
    21601 tcgttaaacc accccatcca tgttttacgt agccttacca taattttatc atttctccgc
    21661 ggtatctata taatccttct attggtggga aacttgtatt ataatatggc tattgaacgt
    21721 ccggtatcat cataaggcgt tctgctgttt tgcatgggtg gatgatttat ctataaaaca
    21781 agatatggag gatgaaaatg gatcattatt atagcgaaaa tccaagggtc caacatgata
    21841 tcggccggat tgaatatagc ctggggaata ttgaactttc atttatcacg gatgcggggg
    21901 tattttccaa ggacagggtg gactatggaa ctcatattct gctgaggagc ctgccaccgt
    21961 tagtgggcaa gattttggat cttgggtgcg gatatggacc catagggatt accctagcta
    22021 ggctcaacca aaattctgag attactatgg tcgatattaa tcagcgggct gtagacctcg
    22081 ctaaaataaa cgttgaacaa aacggcataa caaacgccgg ggtgttaaag agtgacggct
    22141 ttgaaaacct ggacgagagc ttcaacgcta tcatcagcaa tccccccata cgcacgggaa
    22201 agaaggtcat atattcgcta tttgaagaga gcggcaagta tcttcaagac ggtggaagca
    22261 tttatctggt tatacagaaa aaacagggcg ccaaatccgc catggaaaag ttggaccaaa
    22321 tttacggaaa ttgcagtgcc ataaacaaaa aaggcgggta ttgggtttta catagtgtta
    22381 aagtttgaca ggctgcatta aattgaatat ttatttagtt agcagaaaac aataagggtg
    22441 atcattttat gaaaggagtg tttttttgtg acgcagatca atcagatgga actccaaagc
    22501 atacggcacc taattgggga acacgagaca gcataccaaa aactgtccac ctatgcccag
    22561 cgatgtactg acccgcaaat aaaacagcta tttcaacaat cggcacagga tgcccaaaat
    22621 agtaagcaga gattaatgac ttttttagga taaggagtgg tgatgatgca ggaaaaggat
    22681 atggtaaatg atatactatc cacgctaaag agcagcattg caggatatgc ccatattata
    22741 agcgaggccg gtaacgagga attcagacaa accgttcagc aaatgcgaaa cggcgatgaa
    22801 aaatgccagt atgatttgtt taagatagcc caggaaaagg gctactatat acccgcccag
    22861 gcagccagcc ccaacgatct tcagaacgta aaatcgcagt tctccggctg agcatgccgg
    22921 aacatacatt attataaata tggaaccagc tgagccgcag acaatatcgt ctgcggtttt
    22981 acttagaata taactattat ttcacacatt atagcggacc atcatattct gtattagaac
    23041 atgaaggagg ggggaaactt atggcaggta tgatggaggc cttaggagga atgttttgcg
    23101 gtaaaggcgg ggatcagagc ctacttttct ttttcttact gttggtgata atcttctgta
    23161 attgtgggct gttcaacgac ggggatgagc tactattctt cttcttattg ctgattcttc
    23221 tattcggtgg cggatctttt ttccgcggct ttggtgtaca gagcaaggac agctagtagg
    23281 gaaatagttc ggtatctgac ccattccata agcctcgaga cggggaaaat acaaaacttt
    23341 aaaaggtgga gaaaggctgt agatatgaaa ttgtgctatc tatggccttt ttaaattagg
    23401 tacgttagcc gggatataat ccttatggaa aggcctgggg cttttattct attgccttat
    23461 cgtctgtcaa tagctgtcgt cttggtttgg gagcgggata gaggactgtc atcttaagta
    23521 aatttaaatt gaatatacta gatgggaaaa ttgtgataca atctaaaata agaataatga
    23581 tgcggcatta caggtgttgg gtgcttaaag gacgaataaa agcccgcatt ttttaaaata
    23641 tttaagatgg atgacaacag ggacggatta acggaggtat acatatgacg gggatcacag
    23701 acagaataca gggattgagg gaagggatgc tggatagggg tatagatgcc tatatagtcc
    23761 ccagctccga tccgcatatg agtgaatacg tagcggccca ttgggaaggg cgaaaatggc
    23821 tgtcgggatt tacaggttcg gcgggaaccc tggtaatcac ccgggatttt agcggattgt
    23881 ggaccgacgg aagatactat gttcaggcag agaagcagct tcaagggacc ggcatccagt
    23941 tgttcaaggc cggaatggaa ggcgtcaaga gctttaccca atggattgca gataccttgg
    24001 ataagggaag ctgtgtgggg gtagacggga aattattttc tttatcccag gtgcgaaaga
    24061 tggagagccg gttttccaaa aagggtattt ccataaacaa ggagcatgac cttttgatgg
    24121 ggatatggaa ggacagacca ccgatcccaa atgggcctgt atttgtacac gatatcgcct
    24181 atgcgggaag gactgcggcc caaaaaatag aggacgtcag agcaaggatg acccaaaaag
    24241 gcaccgacta tcaccttgtt tccagcctgg atgatatcgc ctggctctac aatatcaggg
    24301 gcagtgacgt agattacaac cctgtagcca tatcctatgc cctggtttca gacgagaggg
    24361 cgtggctatt tataaacggg gaaaaggttt cggagcccgt cagggagcat ctaaaggata
    24421 atgggataga ggtagaggat tatgaagaaa tagaatccca tctgtcgcgg ctgggcgagg
    24481 gcgacacaat tttgctggat gttaacaggg taaatagttg gctttataat gccattcccg
    24541 atggtgtagg aatcgaagag gataccacca taaccaccac cttgaaggcc atcaagaatg
    24601 agacggagat cgaaaaccta aaaagagccc atgtaagaga tggggtcgcc atggtaaggt
    24661 ttctctattg gctggacaac aacatatcca agggggatat caccgaaatt actgtagcgg
    24721 ataagttgga ggagtatagg agccaacaag aaaactacgt gggcctcagc tttccgacca
    24781 tagcggggta tgaggaccat ggcgcaatag tacattatca ggccgatgaa gaatcggcat
    24841 ataccctcgg gaccgatggg atgatactaa tagattctgg gtgtcaatat ttggatggga
    24901 ccacggacat caccaggacc attcctctgg gagccacaac aagtgaagag cggcgggatt
    24961 acaccttgac tctaaagggc aatatcaagt tgactaggac cagatttctt aaaggggcga
    25021 cgggggccaa cctggatatt ttggccagat tgcatctgtg ggagcatggt atagactaca
    25081 agcacgggac aggccatggt gtgggatact ttctaaacgt tcatgaggga ccccagggca
    25141 ttagccccat atcctcaagg gttaggttgg aggcagggat gctggttacc aatgaacccg
    25201 gggtataccg tgaagggagc cacggcataa ggatagagaa tgaattgttg gtagtagagg
    25261 atatagagac ggaatttggt gagtttctta ggtttgaacc aattaccctt tgccccattg
    25321 acttggctcc gatagagctg gatctattgg atgaagggga aaagaggtgg ctcaacgaat
    25381 atcataaatg ggtttatgat attctaagtc cgttattgaa ccaagaggag agcctgtggc
    25441 ttaaggataa gaccagggcg atataacgaa acgagtgggc aggatgtcac ccgcctttat
    25501 aagcggagag ttccgattgg taaaacaggc ggtgagtaca aatttcccgc ctcgttgaaa
    25561 cctgtgtgtt gcaattgcct ccgatgtttt aatatggccc tacacaccta tgggaaaaga
    25621 atatagatga gataggagat agatagatga gcatgaaaat cgttgccgac agctgttgcg
    25681 acgtgaatcc tgaaattgaa gaaaggacgg atatacaatt agtacccctt acaattcaaa
    25741 tagaggataa ggattatagg gacgacaaag accttgatat tgacaacttg attgtggata
    25801 tgagaaattc tccaaaggta ccaaaaaccg catgtccctc gccccaggat tttattgaga
    25861 gatataaggg cggcgaaagc gtgtttgtgg tgaccctatc ctctgcatta agcggaacat
    25921 acaagagcgc catgctggcg aaggagatat ttttagaaga ggtagagaat aagtttatac
    25981 acgtctttga ttccttgagc gcctcggtgg gggaaaccct gatcagcctg catatatcag
    26041 agctaatcaa taagggatta aaggaggttg agattgtcga taaggtaaac gcctatataa
    26101 aggatatgag aaccttcttt accctaaaaa ccctggataa tctggtaaag tccggcaggg
    26161 taagccagtt tgtggcaagg gtgtcctcca tcctgtcagt gaggccaatt atggcgggta
    26221 accggaaggg cgaaatagag gtgcatgaaa agatagtagg ggccagaagg gtgtttaaaa
    26281 ggttggtgga tgtaatcggt gaacacggcc aaaacctgga ggataaaata ttgggcatag
    26341 cccattgcaa tgctttggaa aatgccttgg cctttaagga ggaagtactg aagaggtata
    26401 acttcaagga tataataata gtcaagacgg cgggaataag caccatctat gcaaatgagg
    26461 gcggactgat catcgctttt tgacgggcct gttatctaca gggcattgat ggctacccct
    26521 ttctatttta agcctatgcg ggaatggggc gctgtccgca taggcattat tctattttag
    26581 ggtgtatgga attcatattg caagcatagt tatagaagag gacaatatga agggacttgg
    26641 ttctatgggg gttattccgt atatcctttt tatcattggg tgtattttta tcataaaggg
    26701 cggggatctc ttcgtcgagg ctgccatctg gatagcccgg gtgacgggaa tgtcggaggt
    26761 gctcatcggt gccaccgtgg tcagtgttgc cactaccttg ccggagttga cggtatctac
    26821 gctatccaat ttacaaggtc aggcgtccat ggccatagga aatgcagtcg ggtctaccat
    26881 ctgcaacatc ggtcttatcc tggccgttag ctgtattata aggcccagca ggatccactc
    26941 ccgatttttc catctaaagg gtatagtcct aatttcgtat acccttctat tatgggggct
    27001 gtcatttaag ggtgggcgca taggcaccag ggacggctgg atattgctag tgctcctggc
    27061 gtcttatctg gcaattgatt atggaatagt caaatataag agccccaaca gagcgccgtc
    27121 gctgcttatg cccatggccc ccaaggaatg gcggggacaa tttttaagat ttctagtggg
    27181 gctggtattc atcctggtgg gggctcggct cctggtcaag tacggcatag agatagctag
    27241 attctggcat gtaccggaga ccataatagc ccttaccata atagccatag gcacttcgtt
    27301 gccggagctt gtaacgtcta tgaccgccgc cataaagggt cacgaggatc tctccgtggg
    27361 aaatatattg ggggcaaata tcctgaacat taccctggtt cttggggtat cggcattggt
    27421 ggggcctttg gaagtgcccg cccaatccaa cgcgttcgat ataccggtat cccttgtcat
    27481 ggggctcata ctggttgtgc cgggggtggt taccaggacc atttccaggc ttcagggcgt
    27541 cgtcctccta attggatatc ttgcatatat agccatccta ttcttcgtct tcggatgatc
    27601 cgcgggcaaa tccaatttgc ttgaagtgac tattcctgtg gtatatataa taattctgac
    27661 atagggggta acgggtgggg tatggggaaa tcttttaaga aacagctgtc ttcgtggatc
    27721 tatcagaacg atatag
//
LOCUS       DASRNX010000119         6163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_394791,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000119 DASRNX010000000
VERSION     DASRNX010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394791"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1931
                     /locus_tag="VFD89_04885"
     CDS             <1..1931
                     /locus_tag="VFD89_04885"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZJ57559.1"
                     /translation="AIVGKKVILPLVNKEIPVVADDYAEMEFGTGCVKMTPAHDPNDF
                     EVGLRHDLEQLRVMDDAGVMNEHAAKYKGLDRYDARERIVNDLKDQGLLAKVEDHSHN
                     VGHCYRCDTVVEPIISKQWFVKMEPLAKPAIEAVRDGRVKFIPERYDKTYFNWMENIR
                     DWCISRQLWWGHRIPAYYCKDCHETIVARDIPDKCTRCGSENLKQDEDVLDTWFSSAL
                     WPFSTLGWPDKTEDLDYFYPTSVLVTAYDIIFFWVARMIFSGIEHMGEIPFHHVFIHG
                     IVRDSEGKKMSKSLGNGIDPLEVIHEYGADALRLSLVTGNSPGNDMRFYTERVEASRN
                     FANKIWNASRFIMMNLKDTEPKKPHREELELPDRWIISKYNRLVKEVTENLEKYELGI
                     AVQKLYDFIWNEFCDWHIELAKPRLYEEEGRQRETALYTLTYVLSNTLKLLHPFMPFI
                     TEEIWQHLPHEGESIMISPWPEFDLGETDEEAEEQMEAIMNAIRSIRNVRAEMDVPPS
                     QRAQVIILPSEGYGEVFHKGKMYFERLAYASKVVIGDSEDDIPKNSVSAVADKAQLYM
                     PLEDLVDFNKEMERLRKESDNLKKEIARAGGKLANENFVKKAPPAVVEEERQKLEKYH
                     EMMEKVAERMDALNKMME"
     gene            1952..3256
                     /locus_tag="VFD89_04890"
     CDS             1952..3256
                     /locus_tag="VFD89_04890"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HZJ57560.1"
                     /translation="MNYDEALQYIHGTYKFGSKLGLENIKDLLGRLGDPQRQFKIIHV
                     AGTNGKGSVTSMITNILHETGYKVGMFISPYLENFTERIQVNLEEIPRNDLATATQRV
                     REKVGEMVASGSNHPTEFEIVTAIGFLYFARQNIDIAVVEVGLGGRFDATNIIDEPLL
                     SVITSIGFDHVAILGSTLGEIAFEKAGIIKPGRPVVSYPQLEEASTVIDKAARDRNAP
                     YYEVDGHQIDVKKATLHENVFDFRYGDELYKDLRLNLIGEHQLLNAATALTAIEVVKG
                     LGLQVSKRAVSEGLSKTRWPGRLEKINDNPIIIIDGAHNVAGADALAHTIGTYFAHED
                     ITLILGVLGDKEVDAIINRVCPLADTVITTRPDNPRAMDPEELGQKALIYNDKVIVEP
                     DIIGAIQKGMDIATKQGLILICGSLYLVGTARKYIKGMDKKE"
     gene            3486..3764
                     /locus_tag="VFD89_04895"
     CDS             3486..3764
                     /locus_tag="VFD89_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57561.1"
                     /translation="MEVKPEAYDNETLLGLLQGAFKDWKRAQNICVRQGDIYIRQNSY
                     FDNAENLRDTYLSLLNEARKRDLSLSREELINSILYTESPRTRKQPKL"
     gene            3864..4784
                     /locus_tag="VFD89_04900"
     CDS             3864..4784
                     /locus_tag="VFD89_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57562.1"
                     /translation="MDYKPVVAVANTDDSSISVIDPIAWEEMKRIQLPPNSGPYDLIK
                     LGSCQRILVSLTYADALAYVDLKKGNVLEIMAIGRRPCHLIYDETQNLAFITNSDTDT
                     VSMVSIVPEEKMRLLGQIKVGSMPLGIDISPVSGDIAVANFNTNDITVISAHGFLVKT
                     CIKLKQNPVGVRYSPRRNKLYITCISIVNDNDAGSIVVVDANSCMVLYEIALRDLPGQ
                     LYITGDDRYALVASMGMGGLEIIDICKRKALLKVSTNGMTHGMALDNNEKYVYVTNPD
                     DDSISKVDWRLGKRMATMVVGKEPNGIVFV"
     gene            complement(4795..5316)
                     /locus_tag="VFD89_04905"
     CDS             complement(4795..5316)
                     /locus_tag="VFD89_04905"
                     /inference="COORDINATES: protein motif:HMM:NF025635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HZJ57563.1"
                     /translation="MIDNMNVLADEKLSILYFLGCLGIPLTNGQITRFFIENNIINYF
                     DLQQYIIELTSSGLVDNMETGNSQFFSITAKGSETLSFFRKRVSPILRNAIESYAEKN
                     RDLLKGESQIIADYRKIDHNQYEIICKVTEKDILLMELKLNVPSSSQAKEICENWRCR
                     APEVFKAIVDRLI"
     gene            5753..>6163
                     /locus_tag="VFD89_04910"
     CDS             5753..>6163
                     /locus_tag="VFD89_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57564.1"
                     /translation="MVRGIKKYMAFILILALMILTACAGGNGGNSMPADNQDNEHEAE
                     VTPPKNETEDDDQGEGDNEEEELPEEGQETVPSVDLKELGVNELGKIMVLMYHGIGDG
                     EDVWVRTPENFRKDLKTLYDNGYRAIGMKDYIEGN"
BASE COUNT         1902 a         1210 c         1561 g         1490 t
ORIGIN      
        1 aggcaattgt cggcaagaag gtcattctgc cgctggtgaa taaggaaatc ccggtcgttg
       61 ccgatgacta tgccgaaatg gaatttggca ccggctgtgt caagatgacg cccgcccacg
      121 accccaacga ctttgaggtg ggcctaaggc acgacttaga gcagcttagg gttatggatg
      181 atgccggggt catgaacgaa catgcggcta aatataaggg ccttgataga tacgatgcca
      241 gggagcggat tgtaaatgat ctaaaagatc agggactatt agccaaggta gaggatcaca
      301 gccataatgt agggcattgc tacaggtgcg ataccgtagt tgaacctatc atatccaagc
      361 aatggttcgt aaagatggaa cccttagcta aacccgctat cgaggctgtc agggacggac
      421 gggtaaagtt catcccggaa agatacgaca agacctattt taattggatg gaaaacatca
      481 gggactggtg catatcaagg cagctctggt gggggcaccg catacccgcc tactactgca
      541 aggactgcca tgagaccata gtagccaggg atatacccga taaatgcacg agatgcggca
      601 gcgagaatct aaagcaggat gaggacgttt tagatacgtg gttcagctcg gccctttggc
      661 ctttttccac cctgggctgg ccagataaaa cggaggactt ggattacttc tatcccacca
      721 gcgttctggt aacggcctac gacataatct ttttctgggt agccaggatg atattttcag
      781 gaatagagca catgggggaa attcccttcc accatgtatt catccacggc attgtcaggg
      841 attccgaggg caaaaagatg agcaaatctc taggtaacgg catagacccc ttagaggtaa
      901 tccatgaata cggtgccgat gccctaaggc tcagcttggt aactggaaat tcacccggaa
      961 acgatatgag gttttacacg gaaagggtag aggcctccag aaactttgcc aataagatat
     1021 ggaacgcctc caggtttata atgatgaacc ttaaggatac cgaacctaaa aagccccata
     1081 gggaggagct ggaactgccc gacaggtgga taatcagcaa atataatcgt ctggttaagg
     1141 aagtaacgga aaaccttgaa aagtatgagc tggggatcgc agttcagaag ctctatgact
     1201 ttatttggaa tgaattctgc gattggcata tagaacttgc gaagcccagg ctatatgaag
     1261 aggagggcag gcagcgggag acggcgttgt acaccctgac ctatgtactg tccaacaccc
     1321 taaagctgtt gcatcccttt atgcccttca taaccgagga gatatggcag cacctgcccc
     1381 acgaggggga gagcattatg atatccccat ggccggagtt tgatttaggc gaaacggacg
     1441 aagaggcgga ggagcagatg gaggccataa tgaacgccat caggagcata agaaacgtca
     1501 gagcagagat ggatgttcct ccatcccaaa gggcccaggt aattatttta ccctcagaag
     1561 gatacgggga ggtattccac aaaggaaaaa tgtattttga aaggctcgcc tacgcatcca
     1621 aggtagttat aggcgatagc gaagatgata tcccaaaaaa cagcgtttcg gcggttgccg
     1681 acaaagccca gctctatatg ccccttgagg acttggtgga ttttaacaag gaaatggaaa
     1741 ggctacgcaa ggaaagcgat aacctaaaga aggagatcgc cagggctgga ggaaagctgg
     1801 ccaatgaaaa cttcgtaaag aaagcgcccc ctgcagttgt agaagaggag aggcaaaagc
     1861 tggagaagta tcatgagatg atggaaaaag tagcggagag aatggatgct ctaaataaaa
     1921 tgatggaata agggtgggtg gatttatata tatgaactac gatgaggcac ttcagtatat
     1981 acatgggaca tataaatttg gctctaagtt gggccttgaa aatatcaagg atctgctggg
     2041 caggcttggt gacccgcaaa gacaatttaa aattatccat gtagccggta ccaatgggaa
     2101 gggctccgtt acctctatga taaccaatat cctacacgag accggctaca aggtggggat
     2161 gtttatatcc ccttaccttg agaactttac cgagaggata caggtaaacc ttgaggaaat
     2221 tcccagaaac gatttagcta cagccaccca aagggtcagg gagaaggtag gggagatggt
     2281 tgcgtccggc agcaatcacc ccacagaatt tgaaatagtt accgccatcg gatttcttta
     2341 cttcgcccgg caaaacatcg atatagcggt tgtggaggtg ggtctcgggg gtcgatttga
     2401 cgctaccaac ataattgatg agccgctgct atcggtaatt acctccatcg gctttgacca
     2461 tgtggctata ctgggcagca ccttgggcga gattgccttt gaaaaggcgg ggataataaa
     2521 acccggcaga ccggtggtaa gttaccccca gctggaggaa gcatctacgg ttatcgataa
     2581 agcggcaagg gatagaaatg ccccttacta tgaggttgac ggccatcaaa ttgatgtaaa
     2641 aaaagcaacc ctacatgaga atgtattcga tttcagatac ggggatgaat tatataagga
     2701 tttaagattg aatctcattg gggaacatca actcttaaac gctgctacag ccctgacagc
     2761 catagaagtt gtgaaagggc ttggcttaca ggtttctaaa agggccgtat cagaaggcct
     2821 ttccaaaaca aggtggcccg gccgcctgga aaagattaat gacaatccga ttattatcat
     2881 agacggtgcc cacaatgtag ccggcgccga cgccctggcc cataccatcg gcacttactt
     2941 tgcccatgag gatattaccc taatattggg tgtattgggg gacaaggaag tagacgccat
     3001 aataaataga gtatgcccac tggctgatac cgtcatcaca acaaggcccg ataacccgag
     3061 ggctatggat cccgaagagc tgggtcaaaa agctttgatc tataatgata aggtcatagt
     3121 ggaacccgac attataggcg ctatccaaaa gggaatggat atagcgacca agcaagggtt
     3181 gatcctaata tgcggttccc tttacctagt agggaccgcc cggaaatata ttaagggtat
     3241 ggataaaaag gaatgaggat aaagtgaagt aagaataaaa tgaagtaaga ataagatagg
     3301 gatatagaaa gaaccgatag tggtcttcat atcgcttcat ccgcatagaa tgtaatagct
     3361 atttgtgcgt gcaaatatgc cctactatgg ggaggtatta tacgatatcc tgattgcaaa
     3421 gttctagtag gggatatttg ccgcgggaca tatagccaat acccgataga tgggaagatg
     3481 aagcgatgga agtaaaacct gaagcatatg ataatgagac attgcttggg ctcttgcaag
     3541 gtgcgttcaa ggattggaag agggcccaaa acatatgtgt aaggcaggga gatatataca
     3601 taaggcaaaa cagctatttt gacaacgccg aaaaccttag ggatacatat ctttcccttt
     3661 tgaatgaggc aagaaagagg gatctatccc tatcccgaga ggaattgatt aattctatat
     3721 tgtatacaga atcaccaaga acaagaaagc agcctaaatt atagaaccgg agggctgctt
     3781 ttatagtaca catatttgct ctcccccata taatgaaact agcctgcttt taagggatac
     3841 atttattcat aggggaggtc attatggatt ataaacccgt ggtagcagta gctaataccg
     3901 atgatagcag catttccgtt atagacccta ttgcctggga agagatgaag cgaatacagc
     3961 ttccgccaaa ttccggacca tacgatttaa tcaaactggg gtcctgccaa aggatcttgg
     4021 tgagtttaac ctatgccgat gcactagcct atgttgattt gaaaaagggt aatgtgctgg
     4081 aaattatggc aatagggagg cgtccatgcc accttatata cgatgaaacc caaaacctag
     4141 cctttattac aaatagcgat acggataccg tatctatggt atccatagta ccggaagaaa
     4201 agatgagact tttgggccag attaaggttg gctccatgcc cctaggtata gatattagcc
     4261 ccgtatctgg ggatatagcc gtagcaaatt ttaatacgaa cgatataacc gtaataagcg
     4321 cccatggttt tctagtaaag acatgcataa aactgaaaca aaatccggtg ggggtaagat
     4381 attccccgcg gaggaataaa ctgtatataa cctgtatatc cattgtaaac gataatgatg
     4441 ccgggagcat agtagtggtt gatgcaaaca gttgtatggt gttatatgaa attgcgctgc
     4501 gggatctccc cggacagctt tatataaccg gcgacgatag gtatgcattg gttgcctcaa
     4561 tgggtatggg gggacttgaa ataatcgaca tatgcaaaag aaaagcatta ctgaaggtgt
     4621 ctaccaacgg gatgacacat gggatggcat tggataacaa cgaaaagtac gtatatgtta
     4681 caaatcccga tgatgattct atatccaaag tagactggag attggggaaa agaatggcta
     4741 ccatggtggt gggcaaggag ccaaacggta tcgtcttcgt atgaggtcct agcattagat
     4801 cagacggtca acgatggctt taaacacttc aggagccctg cacctccagt tttcacatat
     4861 ttcctttgcc tggctgctgc tgggtacgtt cagcttaagt tccatgagga ggatatcctt
     4921 ttccgtaacc ttacagatga tctcatattg attatggtct atcttcctgt aatcggcgat
     4981 tatttggctt tcgcccttta aaaggtcccg atttttttcc gcataggatt caatagcatt
     5041 tcggaggatg ggggaaaccc tctttctaaa gaaggatagt gtctcactcc cctttgccgt
     5101 tatgctaaaa aactgggaat tacccgtctc catattatct acaaggccgc tggacgtaag
     5161 ttctataata tactgttgga gatcaaagta atttataata ttattctcta taaagaagcg
     5221 ggtaatctgc ccgttggtaa ggggtatacc caggcaacct aaaaagtata ggatggatag
     5281 tttttcgtcc gccaagacgt tcatattgtc gatcataatt tcacctcata agggaccgtc
     5341 acaaatcttt ggaaagctgc aatattttac tcaaacatta taacgattat agcacaaatc
     5401 gcaaagggca gaaagaggat attctgcgcc taatacacaa tatttgctta tgggacaccg
     5461 ttcacccctt ttatccacta atttggtgga ttctgaatac tatttgagca gaatatacga
     5521 gctaaaaggg attttttgtc catagcattt ttttgctatt tttgataata ttgttcatag
     5581 atagtgtgtt atcatgtata ttagttaaat agtgcgtttt gccggcctat tatattactg
     5641 tatgataaag gtaatgagtt atagaaaaac agtacgcatg ggccgggaag acaaagtttt
     5701 aagaacaaat ctatatgcga aaatgcaaga tattaaacga aagaggatgg atatggtgcg
     5761 agggataaag aaatacatgg cttttatact aattttggct ctgatgatcc ttacggcttg
     5821 tgccggtggg aatggaggta attctatgcc ggcggataac caggataatg agcatgaggc
     5881 ggaagttaca cccccgaaga atgaaacaga agacgatgat cagggggaag gggataatga
     5941 agaggaggaa ctgccggaag aagggcagga gacagtacca tccgtggacc taaaggaact
     6001 tggagtcaac gaactaggaa agataatggt cttaatgtac catggaatag gcgatgggga
     6061 ggacgtatgg gtaaggacac cggaaaactt ccgaaaggac cttaagaccc tatacgataa
     6121 cggataccgt gcaatcggga tgaaggacta tatagaggga aac
//
LOCUS       DASRNX010000120         7012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_554131,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000120 DASRNX010000000
VERSION     DASRNX010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554131"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..519)
                     /locus_tag="VFD89_04915"
     CDS             complement(190..519)
                     /locus_tag="VFD89_04915"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HZJ57565.1"
                     /translation="MIHGEARGESYLGKLAVGAVVVNRLKSPLFPDTLAGVIYERRAF
                     TCVDDGQIDLEADEESYRAAMDALNEIDPTNGCLFYYNPRTATSRWMYKRPTDYTETI
                     GNHVFMK"
     gene            complement(550..690)
                     /locus_tag="VFD89_04920"
     CDS             complement(550..690)
                     /locus_tag="VFD89_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57566.1"
                     /translation="MKKSLCLIALNLMILNFFALPAVARHDYPGVKYTPVSGSLSVTR
                     MP"
     gene            964..2634
                     /locus_tag="VFD89_04925"
     CDS             964..2634
                     /locus_tag="VFD89_04925"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HZJ57567.1"
                     /translation="MLTDIEIAQSTKLKPIAEIAGQAGIDEEYLEMYGNYKAKVRLDI
                     FDELKDNQTGRLLYVTAITPTPAGEGKTCTAIGLTQGLGKLQKNVAVCLREPSLGPTF
                     GIKGGAAGGGYSQVLPMEDINLHFTGDIHAVTSAHNLLAAMIENHITKGNDLGIDPTR
                     ILWKRVMDISDRQLRNIVVGLGGKANGIPLESGFDISVASEVMAILCLATDMTNLKER
                     LGKILVAYTYEGEPVYAKQLKAVGAMAVLLKDAILPNLVQTLEGQPAFVHGGPFANIA
                     HGNNSIIATRLGLRLFDYVVTEGGFAADLGAEKFFDIVTRVSDLKPDAVVLVATVRAL
                     KFHGGVSRKDLNHEDLKALKVGISNLDQHIDNLKNCFGLPVVVAINRFPTDTDAELRL
                     LMGHCQDLGINAALSEVVARGGDGGVELAKAVLNALDEENRFAHAYPLDMPIGEKIEM
                     LAKKIYRADGITYTARARKSLKSLEELGYGDLPICIAKTQMSFSDDPTLKGAPRGWTL
                     NIRDLRVSAGAGFIVVLTGEMLTMPGLPKNPAAEGIDILEDGTILGLF"
     gene            complement(2631..4028)
                     /gene="murC"
                     /locus_tag="VFD89_04930"
     CDS             complement(2631..4028)
                     /gene="murC"
                     /locus_tag="VFD89_04930"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZJ57568.1"
                     /translation="MKKLHIEELKGNHVHFVGIGGISMSGLADILIHKGYTVSGSDIK
                     QTHLTDGLKSRGATIYIGHDASNIGDADWVVYTIAISENNPELLAAKDMGIPIIERAA
                     LLGHIMETYPYSIGVAGSHGKTTTTSMLSVMVQRARLDPTILVGGEVDDIGGNVRIGN
                     SSYFITEACEYNESFLNFEPYMAIILNIDEDHLDYFKDIQGIYKAFEKFTDLVSPKGV
                     VIGCTDDPLVHKLLEDIHIRKISFATENPGDWVARDIGYDDMGHPSYRAVYKGQDMGR
                     FQLQVPGHYNIYNALAATAAAWAIGISAEIIRDSLATFRGTHRRFEVKGSLNDITVVD
                     DYAHHPTEIKATLKAALKYPHKRIWCVFQPHTYTRTEKLFYEFSESFLDADEILVTDI
                     YAAREKAIDGVNSENLAKEIHRRGKSARYMTSFHEAVDFLSQNARPGDLVLTMGAGDV
                     FKVADGFLKPSGESL"
     gene            4300..4384
                     /locus_tag="VFD89_04935"
     tRNA            4300..4384
                     /locus_tag="VFD89_04935"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4334..4336,aa:Tyr,seq:gta)
     gene            4582..5052
                     /locus_tag="VFD89_04940"
     CDS             4582..5052
                     /locus_tag="VFD89_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HZJ57569.1"
                     /translation="MGAFFEYLEVIKKELIVFSTAILPLVELKGAIPVGIGMGLSAMT
                     SFIVAYLGSILPVPILLLFLKPVMAFLKGTRLLAPFANWVERRTNKKVRGVRKYSLLG
                     LFIFVAVPLPTTGIWTGSAIASFLDIRIMHAFPVIALGNLVAGLIIMFLSHQIF"
     regulatory      5307..5562
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            5619..6821
                     /gene="metK"
                     /locus_tag="VFD89_04945"
     CDS             5619..6821
                     /gene="metK"
                     /locus_tag="VFD89_04945"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZJ57570.1"
                     /translation="MNKELFTSESVTEGHPDKICDQISDAILDAILEQDPMARVACET
                     AITTGMVLVMGEITTTCSVDIPGIVRDTIEDVGYISSEFGFDARTCAVLTSIDRQSPD
                     IAIGVCRGDGVNDTCSADESDDTEAIGAGDQGMMFGYACSETPELMPLSISLAQQLTR
                     RLSVVRKEGSFAYLRPDGKSQVTVEYEGDRPVRVDTVVVSTQHSSDVSQETIKRDILE
                     HVIRPVIPEELLDGDTKFLINPTGRFVVGGPQGDSGITGRKIIVDTYGGYARHGGGAF
                     SGKDPTKVDRSASYFARYVAKNIVAAGLAHKCEVGLAYAIGVARPVSVLVDTFGTGKI
                     SRDDIITLINRNFDSRPAAIIRDLDLRRPIYRQTAAYGHFGRVDIQVPWERTDKAESL
                     KEQAKAFI"
BASE COUNT         1854 a         1565 c         1756 g         1837 t
ORIGIN      
        1 taagtgaaca atttcgatgc tgataaaaca tatattccca gatggatttt atcatgaaca
       61 tttggtcttt tgctatggtc gatttgtgta tataatttat catactatac cataaaaatg
      121 caaaaatggg aaggactaaa aacaataatt gcccgaattt agtctgtctt cgggcaatta
      181 ttattgttcc tatttcataa agacgtgatt gcctatggtt tcggtataat ctgtaggccg
      241 tttgtacatc cacctggatg ttgcggtcct gggattgtag taaaataggc atccattggt
      301 cgggtcgatt tcatttaggg catccattgc agccctatag ctctcctcgt cggcctctaa
      361 gtctatttgg ccgtcatcta cacaggtaaa tgcccgacgc tcatatatta ccccggctaa
      421 ggtatcagga aacaatgggg actttagcct attaaccaca acggcaccga cggccaattt
      481 tccaaggtag ctctcgccac gggcctcccc atggatcacc ctggccaaaa gatcaatatc
      541 ctctgacttt catggcatcc ttgtaacgga caggctccca gatacggggg tgtatttaac
      601 ccccggatag tcatggcggg ctactgcggg gagcgcaaaa aagtttagga tcattaggtt
      661 aagagcaatg agacacaaag actttttcac cttttatccc tcctcaagtc tattattcac
      721 cgatatagaa taaaaatgtc tggaactttc aataaaaaaa accaaatttc gcaaatttgg
      781 tatataatga ttattacccg gaagacgtaa ttaccgaact atacatatag tacgtgttat
      841 ccgaaatggc agatcggtcc gccatacggg taaaattcat gtagggtgaa attcatacag
      901 cctttgacag aggcggtaaa actaaaattt aaaaatttat cgaaatgaaa gggcgggatt
      961 tttttgctga cggatattga aatagcgcaa tcgacaaaat taaaacccat agccgaaata
     1021 gcgggacagg ccgggataga tgaagaatac ctggagatgt acgggaatta taaggccaag
     1081 gtgcggctcg atatattcga cgaactcaaa gataatcaaa ccggcagact tctatatgtc
     1141 acggccatta ctccaactcc cgcaggggaa ggaaagacct gcacagccat cggccttacc
     1201 cagggccttg gaaagctcca aaaaaatgtt gcggtctgcc ttagggaacc gtctttgggg
     1261 cccacctttg gcataaaggg cggggccgca ggcgggggct attcccaggt gcttcccatg
     1321 gaggatatta acctccattt tacaggggat atccatgcag tcacctcagc ccataatctt
     1381 ttggcggcta tgatagaaaa ccatataacc aagggcaacg acttggggat tgatcctacc
     1441 cgcattctct ggaagagggt tatggatata agcgacaggc agctgagaaa catcgtggtg
     1501 ggtctcgggg gaaaggctaa cggaatcccc ttagagtcgg gatttgatat ctctgtggcc
     1561 tccgaggtta tggccatttt gtgcctggca acggatatga ccaacctaaa ggaaaggctg
     1621 ggtaaaatct tagtagccta tacatatgag ggagagcccg tctatgcgaa gcagctaaag
     1681 gccgtaggtg caatggcggt ccttttaaag gatgcaatac tgccgaatct ggtacagacc
     1741 ctggagggtc agcccgcctt cgtccatggg ggtccctttg caaatatagc ccatggtaac
     1801 aattccatca ttgcaacccg gttgggcctt aggctttttg actatgtggt gaccgaaggg
     1861 ggattcgcag cggatttagg agccgaaaaa ttctttgata tagtcaccag ggtctcggat
     1921 ctaaagcccg atgcagtcgt attggtggca acggtgaggg cattgaaatt ccatggcgga
     1981 gtatcgagga aggacctaaa tcatgaggac ttaaaagccc tcaaagtggg gatctccaac
     2041 ctggatcagc atatagataa cctaaagaac tgcttcggcc tccccgtggt tgtagccata
     2101 aacaggtttc ccacggatac agatgctgaa ctgcggttgt tgatgggaca ttgccaagac
     2161 ctaggcataa acgcggcctt atcggaagtt gtggcaaggg gcggtgacgg gggagtggaa
     2221 cttgctaagg cggtcctaaa tgctctggat gaggagaaca gatttgccca tgcctatccc
     2281 ttggacatgc ccattggaga gaagatcgaa atgctggcaa aaaaaatcta ccgtgccgac
     2341 gggataacct atacggccag ggcccgaaag agcctaaaat ccttggagga actggggtac
     2401 ggtgacctgc ccatatgtat cgccaagacc cagatgtcct ttagcgatga tccgaccctg
     2461 aagggggcgc ccaggggatg gacccttaac ataagggacc ttcgggtatc ggcgggggca
     2521 gggtttatcg tagttttgac aggggagatg ctcaccatgc cgggcttgcc taaaaaccct
     2581 gcggccgaag gtatagatat attggaggac ggtacaatcc tcggactgtt ttagaggctt
     2641 tccccgctgg gttttaaaaa tccgtccgcg accttgaata cgtccccggc tcccatggtg
     2701 agaaccaggt ccccgggcct cgcgttttgg ctcagaaaat ccacagcctc gtggaaggag
     2761 gtcatatacc tggccgactt tccccgtctg tggatttcct tggccagatt ttcggaattc
     2821 accccatcaa tggccttttc cctggctgcg tagatatccg tcaccaggat ttcgtctgca
     2881 tctaaaaagg attcggaaaa ttcatagaac agcttttccg tcctggtata ggtgtggggc
     2941 tgaaatacgc accatatcct tttgtgggga tatttgaggg ctgccttcag ggtagccttt
     3001 atttccgtgg ggtggtgggc ataatcgtcc actacggtta tatcgttgag gctccctttt
     3061 acttcgaacc gcctatgggt acccctaaag gtggccagag aatctctgat aatttctgcg
     3121 gatataccta tagcccaggc ggccgccgtt gctgccaggg cattgtagat gttataatga
     3181 cccggcacct gtagctggaa tctgcccata tcctgcccct tatatacagc gcggtaggat
     3241 gggtgaccca tatcgtcata ccctatatcc cgggctaccc aatccccagg attctccgtt
     3301 gcaaagctga tctttcttat gtggatgtcc tctagcagtt tatgaaccaa gggatcgtcg
     3361 gtacaaccta tgacaacccc ttttggagac actagatcgg taaatttttc gaaagcctta
     3421 tatatacctt ggatatcctt aaagtaatcc agatgatcct cgtcgatatt aaggattatg
     3481 gccatatagg gttcaaaatt taaaaagctc tcgttgtatt cgcaggcctc ggttataaaa
     3541 tagctgctgt ttccgattcg tacattgccg ccgatgtcgt ctacctcccc gcccaccagt
     3601 atagtgggat ccaagcgggc tctttgtacc atgacggaaa gcatggatgt ggtggtggtc
     3661 ttcccgtggc ttccggccac ccctatggaa tatggatagg tctccataat atggcctaag
     3721 agcgcggccc tttcgattat gggtataccc atatccttgg cggctaatag ttcgggattg
     3781 ttctcactga tcgctatggt atacaccacc caatccgcat cccctatatt cgatgcgtcg
     3841 tgtcctatat agatcgtcgc acccctgctc tttaagccgt ccgtgagatg ggtttgcttt
     3901 atatccgaac ctgaaacggt atagccctta tggattagga tatcagccaa accgctcatg
     3961 ctgattccgc ctatgcctac aaagtgaaca tgatttcctt tgagctcttc tatgtgcagt
     4021 ttcttcatgg gatccactcc tctgaaatca ccgattgttt taatgtatcc attattttgc
     4081 catattatgt atgtccttag ggatttggga caaaatttgc attatctgct atatgcaaat
     4141 ggaaggtttc cggggcaaat gggcaaaata tacaagctaa tgtacaataa tccagtaaaa
     4201 caatcagatt tatttaaaaa tactcagttg acattctgaa tatcatgtac tataattatg
     4261 aatcgtggcc ggtaaaggtg aatggccaca aaagaatatg gaggagtacc caagtggcta
     4321 aagggggcag actgtaaatc tgctggctca gccttcggtg gttcgaatcc gccctcctcc
     4381 accaaagacg gccgatagcg tatcggccat tttttattta ctcatgttgt ctgcgggcat
     4441 acttatatct ttcctgtgcg gagttatgga tttaacctgt ttatctatag gcgtgtcgat
     4501 aatggctgtt ggacgcccgg taccatcata aaagtatttg gggggttatc ccttttccat
     4561 cataaagggg tgtgctgtaa tatgggggct ttttttgaat atttagaggt aattaaaaag
     4621 gaattgatag tgtttagcac cgccatcctg cccttggtag aattaaaggg ggccattcct
     4681 gtggggatag gcatggggct cagcgccatg acatctttta tcgttgctta tctgggctca
     4741 attttgccgg ttcctatcct cttgctcttt ttaaagcctg tcatggcctt tttaaaaggg
     4801 acaagattgc tggcgccctt tgccaattgg gtggagaggc ggaccaacaa aaaggtaagg
     4861 ggcgtaagaa agtacagcct gttggggttg ttcatcttcg tcgcggtccc cctacccacc
     4921 acggggatct ggacaggctc ggctatagcg tcatttctcg atatcaggat aatgcacgcg
     4981 tttcccgtta tagccctagg aaatttggtg gctggcctta taataatgtt tttatcccat
     5041 cagatatttt gaggacaggg actgcatcat actcgaataa acattctggg gacataatta
     5101 atgttggtac ataaagacat tatcatgttt acaccataga tattttggag atgatacctg
     5161 cagcttgact cttcccattt tcctgtgcta taatcaacca ggtttagtta tagacaaacg
     5221 atgggtcaac taaatgtatc tatgggtcag agcctttata gttggagttg tttgtctacg
     5281 gataacaata atcaaatatg agattttcct tatcaagaga ggtggaggga cgggccctat
     5341 gaaacccggc aaccgattga gcctatatat gttgcgaaga agacctttca ttgtaaaatc
     5401 ggctaaacta tccgatttta cgttataaat aatgtatttt tcgtaacata tgaattaagg
     5461 ataatgtttt acgacacctg tgtatcaatg cagaatgttt atcgtaaaac atatagatca
     5521 ggtgccaaat cccacagaat actatattct gaaagatgag ggtattgatg aagacagccc
     5581 tcttaattga agagggcttt tatatttagg agggcgagat gaataaggaa ctatttactt
     5641 cggaatcagt aaccgaagga catccggata agatctgcga ccaaatttca gatgcgattt
     5701 tagatgccat attggagcaa gatcccatgg ccagggtagc atgcgagacc gccataacca
     5761 ccggtatggt attggtaatg ggggagatca ctacgacgtg ttccgttgat atccccggga
     5821 tcgtaagaga taccatagag gacgtgggat atatcagctc cgaattcgga tttgacgcaa
     5881 ggacctgtgc ggtgctcaca tccatcgaca gacaatcccc ggacatagcc atcggtgtgt
     5941 gtcgcgggga cggggttaat gacacatgtt ccgcagacga aagcgatgat acagaggcca
     6001 taggagccgg ggatcagggt atgatgtttg gctatgcctg cagtgagacc ccggagctaa
     6061 tgccgctatc catttcactg gcacagcagc tgacccgcag gctctctgta gtaagaaagg
     6121 aaggcagttt cgcgtatctt cgtcccgacg ggaaatccca ggtgacggta gaatatgaag
     6181 gcgacagacc tgtcagagtg gataccgtgg tagtatcgac ccagcacagc agcgatgtaa
     6241 gccaggagac tataaaaagg gatatccttg agcatgtaat cagacctgtc atccccgagg
     6301 aattattgga cggggatacc aagtttctaa tcaatcccac cggccgcttt gtggtggggg
     6361 gcccccaagg ggattcaggc atcacggggc gtaaaataat agtggacacc tatggcggtt
     6421 atgcacgcca tggcggaggg gcattttccg gtaaggatcc cacaaaggta gaccgatcgg
     6481 cgtcctattt tgcaaggtat gtggcaaaga atatagtagc ggcagggctt gcccataagt
     6541 gcgaggtggg gcttgcctat gctatcggcg tcgcaaggcc cgtgtccgtc ctggtggata
     6601 ccttcggtac ggggaaaatt tcccgcgatg atatcatcac cttaataaac aggaattttg
     6661 actcaagacc cgcagccatc ataagggatc tggaccttcg aaggcccatt taccgccaaa
     6721 cggctgccta cggacacttc ggaagggtgg atatccaggt gccctgggaa agaacggata
     6781 aggcggagtc gctcaaggag caggccaagg cctttatata agcggattta cctattatgc
     6841 ttgtccatta tcccattgcg ccatagagat aatgagctat tgaaaagcgg atgacttcat
     6901 tgatttggct aagaaaactt ggcttatgga atacaaaaaa gccgtagttt aaggcaggac
     6961 gccttaaacc gattccaccg gcaggaggcc ggattggaag cgataggctt tt
//
LOCUS       DASRNX010000121         8763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_395317,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000121 DASRNX010000000
VERSION     DASRNX010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395317"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFD89_04950"
     CDS             complement(<1..568)
                     /locus_tag="VFD89_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZJ57571.1"
                     /translation="MKIAVAQYNPKVGDLEGNASKIIDFIAGAKEQKCDLVIFPELSI
                     TGYPPRDLLLRSAFLKACDRALHRIIGYTGEIGVIVGTVMAGPRDGLLYNSAILIENG
                     NIIGRVDKSLLPNYDVFEEARYFRPSEQIKCLQFQGKRLGVSICEDIWNDEDLFGRLK
                     YPRNILDELYEDEPDIFINLSASPYHYGK"
     gene            complement(573..2078)
                     /gene="thrC"
                     /locus_tag="VFD89_04955"
     CDS             complement(573..2078)
                     /gene="thrC"
                     /locus_tag="VFD89_04955"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZJ57572.1"
                     /translation="MHYLSTRDSKLKIGSMEAIKQGISRDGGLFVPETIPELSDRDLL
                     DMAGADYMERALKVLAPFLTDYNPGDLKEIINSAYGKGDFHHPDISPVKKLNEDFFIQ
                     ELWHGPTYAFKDMALQILPHLMTHAASNTGEDKEIVILVATSGDTGKAALEGFRDVKG
                     TQIIVFYPDEGVSPIQELQMITQQGENVHVAAVKGNFDDTQTGVKSIFTDDGFSKLLG
                     ERGKRFSSANSINWGRLVPQIVYYVSAYGDLIRDNELDPGEEFNVVVPTGNFGNILAA
                     FYAKNMGVPIDRLICASNSNNILTDFIKTGVYDIRRDFYRTISPSMDILISSNLERLL
                     FELCGRQDHRIREWMTELKDTGKYCLDGESLNMLQSTLWGGSADEEQTLKAIRDTYRK
                     HNYIIDPHTAVGKWVHDRYVEETGDTKKTILVSTASPFKFPKDVLKAVIGMESIQDRD
                     DFQILEELASVSGEDIPPGLMELKDKPILHRTTLAAGNMRQAINNFLELDG"
     gene            complement(2513..2923)
                     /locus_tag="VFD89_04960"
     CDS             complement(2513..2923)
                     /locus_tag="VFD89_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57573.1"
                     /translation="MIQAMVGGKGTGKTKTLVNMANKDLERTKGEIVFISGERRRMVE
                     LNHRIRLIKLRDFDIRDFNVFYGFLSGIVAQNYDIERIYIDGLLEYLKEDLVNIGDWF
                     SDINYISEKFNIRFIISMNGDPDSVPTFLKKYIA"
     gene            complement(3175..4992)
                     /gene="typA"
                     /locus_tag="VFD89_04965"
     CDS             complement(3175..4992)
                     /gene="typA"
                     /locus_tag="VFD89_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZJ57574.1"
                     /translation="MNIREDIRNIAIIAHVDHGKTSLVDQLLRQSGVFHENQQVEDRI
                     MDSNDIERERGITILSKNTAVSYNGVKINIIDTPGHADFGGEVERVLKMVDGVLLLVD
                     AFEGPMPQTRFVLSKALELGHPVIVVINKIDRPDARPTEVVDEVLDLLIDLDADEEQL
                     DCPFVFTSAREGTSYRSINENGHGMTTLFETVLEYIPGPKGDDLAPLQMLVSTIDYND
                     YVGRIAIGKIGRGNIEAGQEALLCSYDGGNIRVKITNLYLFDGLKREPVHRADMGDIV
                     AVSGVEGINIGDTICDTEHPEPLPLIEISKPTISMTFSVNTSPFAGQDGTYVTSRNLR
                     ERLFKETYTDVSLRVEETDSPDAFIVSGRGELHLSILIENMRRQGFEFQVSKPRVIYE
                     TIDDRLHEPVERVLVDVPDLYMGTVIEKLGSRKGEILSIGESRGGRVKLEFSVPARGL
                     FGYRSEFLTDTRGEGILNSTFHDYHLYKGDILSRHQGSLVAFDQGEAVTYGLYNAQER
                     GTLFIGPGTMVYEGMVVGENHRSGDIEINVCRKKHVSNVRAAGSDEALRLTPIRDLTL
                     EEALEFIDDDELVEITPKDIRIRKEILNTSLRKRQQKRS"
     gene            complement(5390..6037)
                     /gene="thiT"
                     /locus_tag="VFD89_04970"
     CDS             complement(5390..6037)
                     /gene="thiT"
                     /locus_tag="VFD89_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HZJ57575.1"
                     /translation="MPVSEYLKTVFSKLTELKPVTITILAAFVIAFIAYLALSKEKAK
                     RYNTKVVVYAGLSIAISFVLSYLRLYRWPQGGSITPASMLPLFVFAYFFGIKAGITAG
                     TAYGLLHLVQGPYIIHWAQLLLDYILAYTVLGFAGYFKDNLHKGIILGGFLRFFLSFL
                     SGVIFFGEYAPEGMNTFLYSLLVNLLIIGTETVICLIISLIPQVKTTLNGLKNRV"
     regulatory      complement(6126..6224)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6298..7878)
                     /locus_tag="VFD89_04975"
     CDS             complement(6298..7878)
                     /locus_tag="VFD89_04975"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZJ57576.1"
                     /translation="MKRTAYICIVTLFVTLISGCGGINKPPEGLIKKDIVDDSEDADI
                     LDSLSAGDSENEEDHASPVIRQNASLYFWDKENNKLVSESRKIVATDMDSFVGGVVEG
                     LLRGPQSEELQPVIPSGTTILDIQQIDNIVNINLSGEFLDAEDLLVARTALVNTLTEQ
                     EKIKYVKINIDGRELTRDGTADTQSLGVLVRSTNNINELVAAQGDTRTEDNVKEINWE
                     LFFRDFRGRYLLSEIRPISVKNGGIARAIIEELIKGPIGVSEGLYPVIPQGTQLLDIK
                     LTDDDDGEPGIVALYFSKELKAPFTEEGTSRSKGKEKDPQEVQVREELNKNRESIILS
                     SIVYNLQGINNIDGVKIFYQNRYGDYVDTPLCDVDLTRILTTRDFSNKLGRKIKIYFA
                     NANSTHLIPEYRAMSRENVQIAKTIIDELILGPREDTDQVGIIPPEISMGDIRVWMDT
                     NNTTVMVDLPKGLDGNKMGSTGALMTLYAMVNSLTDPVNTSNIKEVQFLVGGKTVKTF
                     GNLEFGEPFIRNPAIIQE"
     gene            complement(7899..>8763)
                     /locus_tag="VFD89_04980"
     CDS             complement(7899..>8763)
                     /locus_tag="VFD89_04980"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZJ57577.1"
                     /translation="YAITLYFKHRFSDYLVPEIRMAVRDKQSLEQLLVEELLKGPSQH
                     DRTPIMPLGVEVLDVTRKGETVFVNLSKEFTEDINLSEIPGKEEIPEEKRPIVKAEMK
                     RLGIYSIVDSLTELEGVNQVKFLVENRAISYADMDGELEKLMFPESGELEDEQYSNAV
                     LVALARDRSYILTPSKSVELVFEGLVGEPKWDRIYPLLAKETMGKDNLPTKEELAGLW
                     SAVVGNIELENVFIVDEEIKPDGRAFVTVSYNVNFTSGKKEKMRGDMISVVDEENIWK
                     VKLPDFVSEFR"
BASE COUNT         2302 a         2239 c         1611 g         2611 t
ORIGIN      
        1 gttttccata atggtacgga gatgccgata ggtttataaa tatatctggt tcgtcctcat
       61 agagttcatc cagaatattc ctcgggtatt ttaggcggcc gaacagatcc tcatcgttcc
      121 aaatgtcctc acatatggac acacccagcc tctttccctg gaattgaaga cactttatct
      181 gttccgaggg ccggaagtat ctggcctctt caaatacatc ataattgggt aaaaggcttt
      241 tatctaccct ccctataata ttcccattct caatcaaaat ggcactatta tatagtaaac
      301 catcccgagg accggccatt acagtgccta caataactcc tatttccccg gtatagccta
      361 tgattctgtg aagggctctg tcgcaggcct ttaaaaaggc actcctcaac agaagatccc
      421 ggggcggata tcccgttatg ctaagctccg gaaagattac cagatcacat ttttgctctt
      481 tggccccggc gataaaatcg ataattttag atgcgttgcc ctctaaatcg ccaacctttg
      541 gattgtattg tgctacagct attttcataa ttttatccgt ccaattccaa aaaattattg
      601 attgcttgac gcatattacc ggcagccagg gtagtccggt gcaatatggg tttatccttt
      661 aattccatca ggcccggcgg aatatcttcc ccagatacag atgccaattc ctccaatatc
      721 tgaaaatcgt ccctgtcttg gatggattcc atgcctatga cagcctttaa tacatccttc
      781 ggaaatttaa aggggcttgc ggtggacacc agaattgtct ttttggtatc tccggtttct
      841 tccacatatc tatcatggac ccatttccca acggccgtat gcgggtctat gatataatta
      901 tgtttcctgt aagtatccct tatagccttc agggtttgtt cctcatccgc actgccgccc
      961 cataaggtgg attggagcat attaaggctt tccccgtcca aacaatactt tccggtatct
     1021 tttagctccg tcatccattc ccttatccga tggtcttgcc tgccgcagag ttcaaataat
     1081 agtctctcca aattgctgga tattagaata tccatagagg gggagatagt cctgtaaaaa
     1141 tcccgcctta tatcgtatac ccccgtttta atgaaatccg tgagaatatt attgctgttg
     1201 gatgcgcaaa ttagcctgtc tatgggtacc cccatattct tggcataaaa ggctgcaagt
     1261 atattaccaa aattaccggt gggaaccacc acattaaact cttccccggg gtctaactca
     1321 ttatccctaa tcaaatcccc ataggccgat acataataca ctatttgggg caccaatctc
     1381 ccccagttta tggagttagc agaggagaac ctcttacccc gctcccctaa tagcttgcta
     1441 aacccgtcat ccgtaaatat ggacttaacc cccgtctggg tatcgtcgaa gttaccttta
     1501 accgctgcca catgtacatt ttccccttgt tgggttatca tctgcaactc ttggatgggg
     1561 ctaacccctt catcggggta aaatactata atctgagtac ctttaacatc cctgaacccc
     1621 tccagggcgg ctttgccggt atcgcccgaa gtagctacta aaatgactat ttccttatcc
     1681 tcaccggtgt tagacgcagc atgggtcata aggtggggca agatttgaag ggccatatcc
     1741 ttaaaggcat aggtgggacc atgccaaagt tcctggataa aaaagtcttc attgagtttt
     1801 tttactgggg atatatcggg atgatggaaa tcccccttcc catatgcaga attgatgatt
     1861 tcttttaaat cccccggatt ataatctgta agaaacgggg caagtacctt aagtgcccgc
     1921 tccatgtaat ctgctcccgc catatccaaa agatcacgat ctgatagttc ggggatggtt
     1981 tccggaacaa acaatccccc gtctcgggat attccctgct ttatggcttc catggaacct
     2041 atctttaatt tgctatctct ggtactcaaa taatgcatct ttaaattcca ccatcctatg
     2101 tgtccaccca tattaggggt aactataatt attatcgatg taagccatcc gtcataaatg
     2161 gggaggtttc atcaggactc tattcaacta attagattaa acaatgaaaa tatattaaaa
     2221 caatcgatgt aagggcttaa atcaccatac gattgatgtc ggatttcctc ctgctaatta
     2281 tatctcacac acaaggtagc gtcaatacag ccgccaaaac acgggtacat aatctaaatt
     2341 taaaaatgta cgcccccaaa acatggatta tgataaccct ataaaaacca acaaaaaaac
     2401 tcccctaaat agattcagga aagcatcgag ttttaaggct ataaaattaa aaagtttcca
     2461 aaggaaaagc caagggttat ggcgccccct cggctttcgg aatacccttt gactatgcaa
     2521 tatacttctt taaaaaggta ggaacgctat caggatcacc gttcatagaa ataatgaatc
     2581 ttatattaaa tttttctgag atgtaattta tatccgagaa ccaatcgccg atattaacca
     2641 aatcctcttt cagatattcc aaaagtccgt ctatatagat cctttcaatg tcgtagtttt
     2701 gtgctacaat cccgctaaga aatccataaa atacattgaa atccctaata tcaaagtccc
     2761 tgagttttat caaccgaatc ctgtggttca actcaaccat cctgcgtctt tcaccgctga
     2821 tgaagacaat ttctcccttg gttctttcca aatccttatt ggccatgttc accaaggtct
     2881 tggtcttgcc ggtacccttg ccaccaacca tcgcctgtat catgttgacc gcctcccacc
     2941 atattatttt tttggactta gcccggtaaa attatatcaa atacaagcct caattggctt
     3001 gcttcacacc gattcaataa acgcattgag taaagcctac tggttttact tccttgattg
     3061 cgttaattat accatactac aattttcttt acaataagca taccggaaag gccaaacatt
     3121 acttatgccc tgaggcatgg aaaggtacag gcataccctt aacccgcccg tggcctagct
     3181 tctcttttgt tgacgctttc tcagggaggt gtttaggatc tcctttcgta tccggatatc
     3241 cttcggggtt atttccacca attcatcatc atcaataaat tccagggctt cctccaaggt
     3301 caaatcccgt atgggggtaa ggcgcaatgc ctcatcggag cccgcagccc tgacatttga
     3361 tacatgcttt ttcctgcata cattaatttc tatatcccca gagcgatgat tctctcctac
     3421 aaccataccc tcatatacca tagttcccgg tcctataaag agggtccccc tctcctgagc
     3481 attgtataat ccatatgtta ccgcctcacc ctggtcaaaa gctaccaatg aaccctggtg
     3541 ccgggacaga atatccccct tatagaggtg atagtcgtgg aaggtggaat ttaaaatccc
     3601 ctcacctctg gtatctgtca gaaattcgga tcggtatcca aacagtccgc gggccggtac
     3661 ggaaaattcc aacttaacac gtccgcctct gctttctccg atgctgagaa tttcgccttt
     3721 tcggcttccc agtttttcaa ttactgttcc catatataaa tccggcacat ccaccaagac
     3781 cctctcgaca ggttcatgga gcctatcatc aatggtttca tagattactc tgggctttga
     3841 tacctggaac tcgaaaccct gccgtctcat gttttcgatt aaaatggaaa gatgcaactc
     3901 ccccctcccg gagactatga aggcatcggg agaatcggtt tcctcaaccc tcaggcttac
     3961 atcggtatat gtctccttaa acaggcgctc cctgaggttg cgtgaggtca cataggtccc
     4021 gtcctgtccg gcaaaggggc tggtattcac cgaaaaagtc atggatatgg tgggttttga
     4081 tatctcaata agtggcaagg gctcgggatg ctctgtatcg catatggtgt cacctatatt
     4141 tataccctcc acccccgata ccgctacaat atcccccatg tccgctcgat ggacgggttc
     4201 tctctttaat ccgtcgaaaa gataaaggtt ggttatcttg acccttatat ttccaccgtc
     4261 ataactacac agtagggcct cttggcccgc ttctatattc cctcttccga ttttgcctat
     4321 ggctatcctt ccaacataat cgttataatc tattgtggat accaacatct gaaggggcgc
     4381 aagatcatcc cctttcggcc cgggtatata ctctaatacc gtttcaaaaa gggtagtcat
     4441 tccatgtccg ttttcattga tagatctgta cgatgtaccc tccctcgccg atgtaaatac
     4501 aaacggacaa tccaattgct cttcatctgc gtccaaatct atcagcagat ccagcacctc
     4561 gtcgactact tccgtcggcc gtgcatcggg cctgtcgatt ttattgatga ccactatcac
     4621 gggatgtcct aattccaggg ccttagaaag cacaaatcta gtttggggca taggcccctc
     4681 aaaggcgtcc actaatagga gtactccatc taccatcttc agcacccgct ccacttcacc
     4741 gccgaaatcg gcgtgccccg gagtatcaat gatatttatc tttactccat tgtatgatac
     4801 ggcagtgttt ttagacagta tggtaattcc gcgttccctt tctatgtcgt tggaatccat
     4861 aatcctgtct tcgacctgtt ggttttcgtg aaaaaccccg ctttgtctca gcaactgatc
     4921 caccagggag gttttcccat gatcaacatg ggcaataatt gctatatttc taatgtcttc
     4981 tctaatattc ataaattttg gcgttaagcc ccacaaccta aatcatgagg gtaaataccc
     5041 gttctccttt cttaaatagg cttagctgat atcccaattt cctctaactt gcccaaagcc
     5101 caaacatatg cgacaattct aatcttatcc gttgtgccgg tcaataaaac aaatggtgat
     5161 tatcagatga tcccttcttc accgggcgcc tatggacaaa taatatgtaa actaaactta
     5221 tctacatatg ataagtttag tagttgaagt tgtttgtctg cattcgatac ctcgttgatt
     5281 atagcagtcc gccgcttcaa aatcaaatac atttcctact caaaactata tatttttaat
     5341 ttgaagtcca tataaaaaca gggtcaaatt tgaccctgtt tttatctttc tacaccctat
     5401 ttttgaggcc attcaacgtg gttttcacct ggggtattag ggatattatc aggcatatca
     5461 cggtctctgt cccgataata aggagattta caagcagcga gtacaggaac gtattcatgc
     5521 cctcgggtgc gtattcccca aagaatatca ccccggacaa gaacgagagg aaaaaccgca
     5581 aaaatccccc caggattata cccttatgaa gattgtcttt aaagtatccg gcaaaaccaa
     5641 ggacggtata agccaaaata tagtccaata agagctgggc ccaatgaatt atataggggc
     5701 cctggacaag atgcaagagg ccataagccg ttcccgctgt tattccggcc ttaatgccga
     5761 aaaagtatgc gaatacaaat agaggtaaca tgctggctgg ggttatcgat cccccctgag
     5821 gccacctgta taatctcagg taggacagga caaaggaaat tgctatggat agtcccgcat
     5881 ataccaccac ttttgtatta taccgtttag ccttttcctt tgaaagcgcc aaataagcaa
     5941 taaaggctat aacgaatgcg gcgagtatag ttatcgtaac tggttttaac tctgtcagct
     6001 ttgaaaatac tgtttttagg tattctgaaa caggcatgga ttcaccccct tccacaaaga
     6061 tattgcgtac gcaattgggg gttaataaat aagaaacgca agctaatcct gcttgcgcgc
     6121 aatgttgtat gcattacacg cttccctccg ttggaattat cctacaggtt ccaagggtcg
     6181 gtgaatatca ccatctcagc cattaagctc ccctagcgca tacattttat tcatttgtgc
     6241 atctattgta atccatatgg atcggtttgt caaaatttcc ttgctatctc tttccgctta
     6301 ttcttggatt atagcgggat ttcgtataaa aggctcacca aactcaagat tcccaaaggt
     6361 tttcacggtc tttcccccta ccaaaaactg cacctctttg atattggatg tattgacagg
     6421 atccgtaagg gaattcacca tagcatacaa ggtcattagg gcgcctgtgc tccccatctt
     6481 gttaccgtct aaccccttgg gcagatctac cataaccgta gtattatttg tatccatcca
     6541 cacccttata tcccccatgg atatttccgg gggtatgatc cctacctggt ctgtgtcctc
     6601 cctgggaccc agtatcagct cgtctatgat ggtctttgct atctgaacat tttctctgct
     6661 catagccctg tattcaggga ttaggtgcgt ggaattggca tttgcaaagt atatctttat
     6721 ttttctgccc agcttattgg aaaaatccct ggtagttaag attctagtta aatctacatc
     6781 gcataggggg gtatccacat aatcaccgta cctattttgg tagaatatct ttaccccatc
     6841 aatattatta atgccctgta aattatatac aatagaactt agaataatcg actccctgtt
     6901 cttattcagt tcttccctta cctgaacttc ctgtggatcc ttctctttac ccttgcttct
     6961 ggaggtcccc tcttcggtga agggggcctt caattctttt gagaagtata gggctactat
     7021 tcccggttca ccatcgtcgt catccgtcag ctttatatcc agcagctggg ttccctgggg
     7081 gatcaccggg tagagcccct ctgacacacc tataggccct ttaataagtt cctctattat
     7141 agccctggca atgcctccat ttttaacgct tatgggcctt atttcagaca ataggtatcg
     7201 ccctctaaaa tcacggaaaa agagctccca atttatttcc ttaacattat cttcggtcct
     7261 ggtatcccct tgggcagcta ccagctcgtt tatattattg gttgatctga ccaacacccc
     7321 aagggactgg gtatccgccg taccgtctcg ggttaattcc cgtccgtcga tattgatctt
     7381 cacatatttt attttttcct gctctgtaag ggtgtttacc aatgcagtcc gtgcaaccag
     7441 caggtcctcc gcatccagaa attcaccgga caaatttata tttacaatat tatcaatttg
     7501 ctgtatatcc aagattgtgg tcccgctggg aatcaccggc tgcagttcct ccgattgcgg
     7561 tcccctgagc agtccttcaa caaccccacc aacgaatgaa tccatatcgg ttgctactat
     7621 ttttcgtgac tcactcacta acttattatt ttccttatcc caaaaataca atgacgcatt
     7681 ctgtcgtata acgggactgg cgtgatcctc ctcattttcg ctgtcccccg ccgataaact
     7741 gtccaatatg tccgcatctt ccgaatcatc gactatatcc ttttttatca atccttccgg
     7801 gggcttattg attcccccgc aaccggagat taaggtaaca aacagagtta ctatgcagat
     7861 atatgctgtc cttttcaagt tgttcccccc ctgttccatc aacggaactc actgacaaag
     7921 tcaggcagct taaccttcca gatattttcc tcatccacta cgctaatcat atccccccgc
     7981 attttctcct tcttcccgga tgtaaagtta acattatacg atacagttac aaaggctctg
     8041 ccatccggtt ttatttcctc gtcgacaata aatacgtttt ccagctcgat gttccccacc
     8101 actgccgacc acaaaccggc tagttcttcc ttagtaggga ggttatcctt acccatggtc
     8161 tcctttgcga gcaacggata aattctgtcc cacttaggtt ctcctaccaa accttcgaat
     8221 accaattcca cagatttaga cggggttaga atgtaagacc tatcccttgc aagggctacc
     8281 aacaccgcgt tggaatattg ttcgtcctcc aattcccccg attccggaaa catcaatttt
     8341 tccaattccc catccatgtc ggcgtaggat atggcgcggt tctcaaccaa aaacttaacc
     8401 tgattaacgc cttctaattc agtcaacgaa tccactatgg aatagatgcc aagccgtttc
     8461 atctcggcct ttactatggg ccttttttcc tcaggtatct cttctttacc gggtatttca
     8521 gagagattga tatcttcagt aaattctttg ctcaaattga caaatacggt ttcacctttt
     8581 ctggtcacat ccaggacttc tactcccaaa ggcataatag gggtccgatc gtgttggctg
     8641 ggtcccttca agagttcctc gaccaacagc tgttccaaag attgcttatc cctgactgcc
     8701 attctaattt ccggtaccaa ataatcagaa aatctatgct taaagtaaag ggtaatagca
     8761 tac
//
LOCUS       DASRNX010000122         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_554644,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000122 DASRNX010000000
VERSION     DASRNX010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554644"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFD89_04985"
     CDS             complement(<1..298)
                     /locus_tag="VFD89_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZJ57578.1"
                     /translation="MKFWRGSTNIRKELAEVERYIRGALRTRQVTLAEGLKELLNAGG
                     KRLRPALVLISGQFGEYNSKKLMPLAAGIEILHMATLVHDDIIDDADIRRGIPTI"
     gene            complement(332..838)
                     /locus_tag="VFD89_04990"
     CDS             complement(332..838)
                     /locus_tag="VFD89_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HZJ57579.1"
                     /translation="MKKTRKLTLLGLLISQALVLHYIEGFIPVLAPGAKLGLANIITM
                     ITLVLFGFKEAMAIVIIRSVLGPLLGGSITGILYSLSGGLLSGIVMALLYINLRDYFS
                     FMGISTAGAVFHNIGQLLAASWIFNTIGILYTYLPILMIAAIATGYFNGLISRYILGY
                     LKNKAQPG"
     gene            complement(849..1250)
                     /locus_tag="VFD89_04995"
     CDS             complement(849..1250)
                     /locus_tag="VFD89_04995"
                     /inference="COORDINATES: protein motif:HMM:NF018685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HZJ57580.1"
                     /translation="MRPKIGDIFVYIFIIVLVGLSFLGLKTMGNRGDRKMVQIELDGE
                     IIESVEMSETEPEGTTREIRIDTGDGGYNIVRISAGDVGITDANCPDRLCVISPRINT
                     PGQSIVCIPHRLIVRIIGREPEDGAVDDTAS"
     gene            complement(1267..2331)
                     /locus_tag="VFD89_05000"
     CDS             complement(1267..2331)
                     /locus_tag="VFD89_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZJ57581.1"
                     /translation="MYIRLKRFAAISFLLTILSIFAAGCTIGDVRELNRDIFALDTVI
                     TLKVFGGRAVKKALDKAVDRIREIEDRMSPTIESSDVSKINRASGTEPVKVHDDTFYV
                     IKRALNYGELSDGIFDITIGPIVNLWDIKSENAVVPDEAEIKSRLSLVDYRKVRIDED
                     NMTVLLEEKGMAMDLGAIAKGYAADEATRILKEAGVKSALLNLGGNVITIGGKPDGSS
                     WRIGLQDPRSQETGRNHFAIVEVQGATVVSSGDYERFMADVYERTGERYHHIFDPKTG
                     YPAQSGLMVSTIISSSSIDADALSTILFIMGYEDGFRTIGELGNFEAMAVTSDKDVYI
                     TGGLKDGIIPSNDLYNIVEP"
     gene            complement(2475..2615)
                     /locus_tag="VFD89_05005"
     CDS             complement(2475..2615)
                     /locus_tag="VFD89_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57582.1"
                     /translation="MTIKSDGQIPSTSEPGYTVKPGTVVECILGLTVMADGIIALFLL
                     ES"
     gene            complement(2622..>3982)
                     /locus_tag="VFD89_05010"
     CDS             complement(2622..>3982)
                     /locus_tag="VFD89_05010"
                     /inference="COORDINATES: protein motif:HMM:NF018488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HZJ57583.1"
                     /translation="DMMLMATRDTAKYRNKGKKSTHINTVMGEISYSRRIYKYRGDNG
                     KSRFVYLLDEYLNNETIGRSSSNLAEKLVERTLEESYRKAADAIVSTTGANLSHTTAW
                     NIIQKVGSKLKDEEEHLVDRYEQGKLKGDREVDILFQEADGVWLSMQGKDRPKRGSKR
                     EMKVAVNYEGFKKRKGQKEGYQVHNKTACAGFHKSRDFKKMWEAKVAEQYNIDEIKVR
                     IVNGDGDPWIKPDLGHDDVHFQLDPFHISREVIRRVPDKNQARKLNKLLKEGKIDESF
                     EYLTGLLIEYTHEEEKAKKLERLYNYLSNNREGLIPYRLRGLELPKLPDGLEYRGMGT
                     MEGNVCDMITLRMKNRKMSWSWEGANNLARLLAARTSGTLYEQLDRAFDGTVSERMLD
                     EIIDIIQLSTAQANRKPKKSNIYPMHRAPMPYDGQPLTLGRKAIRDLVENRVVSDLNF
                     LY"
BASE COUNT          984 a         1113 c          694 g         1191 t
ORIGIN      
        1 gtatggttgg gataccccgc cggatatcgg catcatcgat gatatcgtcg tgtaccaggg
       61 tagccatatg taaaatctcg atacccgccg ccaaaggcat gagttttttg ctgttatatt
      121 ctccaaattg tcccgatatc agcaccaagg ccggcctaag ccttttgccg cccgcattca
      181 agagctcctt taatccctca gccagcgtta cctgcctggt cctcaaggcc ccccgaatat
      241 acctctctac ctctgccaat tctttgcgga tattagtgct ccctctccaa aatttcatct
      301 ttagctatcc tcccaacaac cgtatcccac atcacccggg ttgcgccttg ttttttaagt
      361 atcccaatat atagcgggaa atcaatccgt taaaatagcc cgtagctatg gccgctatca
      421 tcagaatggg caaatacgta tacagaatgc ctattgtatt gaaaatccat gaggcagcca
      481 acagctgacc tatattatga aatactgccc ctgcagtact tatacccata aaagaaaaat
      541 aatcccttaa atttatataa aggagtgcca taactatccc gctcaagagc ccgccggaca
      601 ggctatacaa gatcccggtt atgctgcccc ccaataaggg gccgagtaca gacctgataa
      661 ttacaatagc catggcttcc ttaaatccaa ataagaccaa ggtaatcatg gtaattatgt
      721 tggccaatcc cagttttgca cccggggcca ataccggaat aaaaccctct atatagtgca
      781 gtaccaacgc ctgggaaatc agcagcccca atagtgtcaa cttcctcgtt tttttcatca
      841 tccctacctc acgatgcggt atcgtccaca gccccgtctt ccggttcccg gccaatgatc
      901 cttactataa gcctatgggg aatacatact atggattgtc cgggggtatt aatcctaggc
      961 gaaataacac atagtctatc gggacagttg gcatctgtta tccctacgtc accggcggaa
     1021 atccttacaa tattgtatcc cccgtctccg gtgtcaatcc taatttccct ggttgtccct
     1081 tcgggttctg tctcggacat ctccaccgat tctattatct ccccatcaag ctctatctgg
     1141 accatcttcc tatccccacg attgcccatg gttttaagac ctaaaaagct taggcccacc
     1201 aaaacaatta taaagatata gacaaaaata tctccaattt tgggtctcat ctatttcctc
     1261 caggctttac ggttcaacga tattatagag gtcgttcgag ggaatgatcc catcctttaa
     1321 tcccccggtt atatatacgt ccttgtccga cgttacggcc atagcctcga aattgcccag
     1381 ctcccctatg gtcctaaacc catcctcata tcccattata aacaatatgg tagacagggc
     1441 gtcggcatct atggaggatg aagagattat cgttgagacc atgagcccgc tctgtgccgg
     1501 ataaccggtt ttcggatcga agatgtggtg atacctctcc ccggttctct cgtagacatc
     1561 tgccataaac ctctcgtagt ccccggagct gacaacggtg gccccttgca cctccactat
     1621 ggcgaaatgg tttcgacccg tctcctggct tctgggatct tggagaccta tcctccagga
     1681 actcccgtcg ggtttcccgc cgatggttat tacgttgcct cccaaattta agagggcgct
     1741 ctttaccccc gcttccttta gtatcctagt cgcctcatcg gccgcatagc ccttggctat
     1801 agcacctaag tccatggcca tccccttttc ctctaaaagg acggtcatat tgtcctcgtc
     1861 tatcctaacc ttcctataat ccaccaatga gagtctgctc tttatctctg cttcgtccgg
     1921 aactactgca ttttccgatt tgatatccca aagattcact atgggcccaa tggtaatatc
     1981 aaatatacca tcgctcaatt ccccataatt tagggctctc tttatcacat aaaaggtatc
     2041 gtcgtggacc ttaacgggtt ctgtcccgga tgccctgttg atcttcgata cgtcgctgct
     2101 ctcgatggtg ggactcatac gatcctctat ctcccggatc ctgtccacag ccttatccaa
     2161 ggccttcttc acggccctgc ccccaaaaac tttgagggtt attacggtat ctaaggcaaa
     2221 gatatcccta ttgagctccc ttacatcccc tattgtgcac cctgccgcaa agattgataa
     2281 tatcgttaac aaaaaactaa tagccgcaaa cctctttaac cttatataca ttaaaaaccc
     2341 acaccaactt catcagaatt tgtcccacac aatcatttta ttatattatc acagtcctat
     2401 ttttttcacc agtattttca tataagtatg tgtcaagaat tcgttggcac gttttccctt
     2461 acaaaatcat ccatttagga ttccaacaaa aataatgcga ttataccatc tgccattact
     2521 gtcaagccta aaatgcactc cactaccgtt ccgggcttga cagtgtagcc cggctccgat
     2581 gtcgatggta tctgtccatc gctttttatt gtcattaaat cctaatataa aaagttaagg
     2641 tcactaacca ctcggttttc aacaaggtct ctaatagctt ttctacccaa tgttaagggt
     2701 tgaccatcat aaggcatggg ggcgcgatgc attggataga tattgctctt cttaggcttt
     2761 ctattggcct gtgcagtcga cagctgtatg atatctataa tttcgtccaa catcctctca
     2821 gatacagtac catcgaatgc tcggtccaac tgctcataga gggtaccgct ggttctagca
     2881 gccaataacc tggctagatt attcgcccct tcccaagacc aactcatttt ccggttcttc
     2941 attctaagcg tgatcatatc gcacacattg ccttccatag tacccatgcc cctatactcc
     3001 agcccatcag gcaatttagg tagttccaac ccccttagcc tgtacggtat tagcccttcc
     3061 cgattgttgg acaaatagtt atagagtctc tccagcttct tagccttttc ctcctcatgg
     3121 gtgtattcta ttaacaatcc ggttagatat tcaaatgact cgtctatctt accttccttg
     3181 agcagcttat tgagctttct agcttgattt ttatcaggca ctctccttat tacctcgcga
     3241 gaaatatgga aaggatcaag ttgaaaatga acatcatcgt gccctaaatc gggctttatc
     3301 caagggtcgc catcaccatt aacgatccta accttaatct cgtctatatt gtactgctca
     3361 gctaccttag cctcccacat tttcttaaaa tcccgactct tatgaaatcc tgcgcaggca
     3421 gttttattat gtacctgata accttctttt tgccccttac gcttcttgaa tccctcatag
     3481 ttaacggcga ccttcatctc ccttttactg ccccttttag gcctatcctt gccctgcatg
     3541 gacaaccaaa ccccatctgc ttcctggaat aaaatatcta cttcacgatc accctttagc
     3601 ttgccctgtt catatctatc gacaaggtgc tcctcctcat ccttaagctt cgagcccact
     3661 ttctgtataa tattccatgc cgttgtgtgg ctaagatttg cacctgttgt actaacaatg
     3721 gcatccgccg cttttcggta tgattcctct agggttcttt caactaactt ctctgccagg
     3781 ttgctggacg aacgaccaat agtctcatta tttaaatatt catctagcaa atacacgaat
     3841 ctacttttgc cattatcccc gcgatacttg tatatccttc tactgtatga tatttccccc
     3901 attaccgtat tgatatgggt gctctttttt cctttatttc tgtatttggc cgtatcacgg
     3961 gttgccatca gcatcatatc ca
//
LOCUS       DASRNX010000123        11082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_395429,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000123 DASRNX010000000
VERSION     DASRNX010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11082
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395429"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1835)
                     /locus_tag="VFD89_05015"
     CDS             complement(<1..1835)
                     /locus_tag="VFD89_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HZJ57584.1"
                     /translation="MLDLKVSINDDFWSRYIALVRDTVIPYQWEALNDRIADADPSGA
                     IRNFRIAAGEEEGDFYGMVFQDSDVAKWLEAVAYRLEAFPDPKLEDIADEVIDLIGRA
                     QEEDGYLNTYYSIKGLDDRWSNLHECHELYCAGHMIEAAVAYYKVTGKRRLLDVVCKF
                     ADYIDTVFGVEADKIKGYPGHQEIELALVKLYKVTENEKYLDLAKYFLDERGRKPFFF
                     DIEWKKRGKTSYWTGDNSEAPGKGKEYYQAHLPIRQQTEAVGHSVRAVYMYASMVDVA
                     METGDQELMEACRTLWENTVSKRMYITGGIGSQVHGEAFSFDYDLPNDTAYAETCAAI
                     GLIFFAKRMLDTEPKGEYGDIMERALYNGVISGMSLDGKQFFYVNPLEVDPEACEKNG
                     NMKHVKPVRQKWFGCACCPPNVARLLASLGHYVYRVNDNTIYAHLYMGSSLEMTVGGD
                     KVRIDQTTDYPWDGRIDIRVGLDGSKDFDLALRIPGWCQNPYISINGEAINLADIVVD
                     GYATIRRRWEDGDKIELVLPMEPVRMRANPMVRANVGKVAVQYGPIVYCLEEVDNGPN
                     LHALVLPEDSKLEATFKEDLLGGVNVITAQGEKPDEELWGQELYRA"
     gene            complement(2081..5815)
                     /gene="pglX"
                     /locus_tag="VFD89_05020"
     CDS             complement(2081..5815)
                     /gene="pglX"
                     /locus_tag="VFD89_05020"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF033452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HZJ57585.1"
                     /translation="MTNTALRSFAMYAREKLIEGIKDRASMMGLTEDGIQLPLNKMKS
                     HDEVLVFKTDSVEPFIISGDGIGQYRALLKELDDRKRYPNHQTAYKELVEDAACTWFI
                     RFIVIRFMEVNGYLPGGIRALSSKMRGLNNPELVDRYQDAGLDLTDTEIRQLEAWKKE
                     ASPISTDRAFRLLFIKLCQAMNQYFPILFEKVDKYAELLPIISYSDPSSVIYKLVHQI
                     KEEHFDIESWEKRGGVEVVGWLYQYYNTGPRDRVVDVLSKTTIRKRDIPAATQVFTPD
                     WIVRYMVDNSLGRYWLERNPHSPIKNFLDYLMPGDIPVVKEQILPGEIKVFDNAMGSG
                     HCLSYAFDVLLMIYKSQGYTAREASRLILEKNLYGLDIDRRACGLARFTLLMKARRYN
                     DGILYSDLKDNLTYFEDSSGIKMEVLDHLGLNMDKDRREIAKEKIKYLIGKFKNATEI
                     GSILRIDKIHTDPLLELIQDYAENSDYIENSDYTGDSVHIGNRDRTGDSDRTGDSDHT
                     GNRDRTGDRDHAGSSETVIRESGIRKTQNQLRKLVKISSMLTGKYHIMVTNPPYLNKP
                     DKRLKDYIWNNYRDYKGDLFSVFIYNNLKMSKKGGYSAYMSPFVWMFIRSHERLREHI
                     IRTKSIVCLIQMEYSAFGDATVPLCTFVVQNNMAMGKGKYIKLSDFPGGMEVQRLKAL
                     EAIRNTQSLHYYEIHQNDFEKIPSMPIAYWAGENIIRAFLKGEPAGSLIEAKVGLQTG
                     NNDRFLRLWHEVDIGKISFSTKSAEASMDSKAKWFPYNKGGMRRQWYGNYDYVVNWEN
                     DGCEIRGFVDSNGKRRSAIRNPDYYFKESITWSLVTSGGFSIRYREAGSIHDVSGMSA
                     FTGDHRRLLYMLGLMGSKVSNYIFKMLNPTINLQVGNFSDFPVLFAHEERVIKLVEEN
                     ISIAREEWNSYEEAWGFSVHPFLSRDGLATIEDAFNAWARVAKDRIERLKRNEEKLNG
                     IFIKLYGLNDKLSPNLDTKDITLRDADIARDVRSFISYGVGCMLGRYCLDFGGLTFAG
                     GEWDVNKYKRFKPSGDNILIITDDDYHIEDITDKFIEFIRVSFGEGTLENNLGFIAGG
                     LGGSGTPRRIIREYFLKDFYRDHVRAYQKRPIYWLYDSGRQNGFKALIYIHRYDRNIT
                     ERVRINYLHKMIGVYRDVLNSLKEDTGYYEDGENIAQVKKRYQKISRQIQECMDFDTK
                     LKELARSRIGIDLDHGIRVNHKKVQTNPDNKNLKVLAKIN"
     gene            6283..7611
                     /locus_tag="VFD89_05025"
     CDS             6283..7611
                     /locus_tag="VFD89_05025"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HZJ57586.1"
                     /translation="MCDTMVALGNSTRDGVTIFAKNSDRQPNEPHIMIRVPAADHEEN
                     SKVKCTYIEVDQVPHTYEILLLKPSWLWGCEMGCNEFGLNIGNEAVFTKEPYGEDALT
                     GMDMIRLALERCKTAKEALMLIIGLLEEYGQGGNCGYEKDFTYHNSFLIADSDSAWVL
                     ETAGIYWVAEKVKDVRSISNCLSIGSEFDLHHPDLIKNAVDKGWCKSEDDFNFAKCYT
                     EPIVTHFSGSKARLATSTSILKQNKGQITVDTMKNILRSHVGRVDANPFKKSSLKSVC
                     MHGGFIFGDHATGSYVASIQDGLPTYWITGSSTPCISLFKPYWMLDEEEFLFEEDEED
                     KALEYWHVREEFHRMVIENRIQDLDRYIKKRDEIERDLSHMVGALDMENPNTGVLRDI
                     MDKAFREEEELILDFLARNRDNPVRIKGNPYFRRYWRKQNEKLRTQTPMT"
     gene            7873..8859
                     /locus_tag="VFD89_05030"
     CDS             7873..8859
                     /locus_tag="VFD89_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HZJ57587.1"
                     /translation="MSKKTIIGEPLPNIPWEDRPEGCNDVMWRYSGNPVIPRDLIPSS
                     NSIFNSAVVPFEGEFAGVFRCDDKRRVMQIHTGRSKDAINWELENDPISFICDDPEIG
                     NFVYGYDPRVCKIDDRYYVTWCNGYHGPTIGVAYTYDFKEYYQLENAFLPFNRNGVMF
                     PRKINGNFAMLSRPSDNGHTPFGDIFYSESPDMTYWGKHRHVMATSEGWQATKIGAGP
                     IPIETTEGWLMIYHGVLTSCNGFVYSVGAALLDIDEPWKVLYRTEPYILSPQTLYECV
                     GDVPNVAFPCAALADSETGRIAMYYGGADTVTCVAFTQVDLLIDYIKNNSKV"
     gene            complement(9063..9878)
                     /locus_tag="VFD89_05035"
     CDS             complement(9063..9878)
                     /locus_tag="VFD89_05035"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HZJ57588.1"
                     /translation="MHLIAVDLDHTALDNLYELNDVTAEALKKLKALGHRVIIATARP
                     SCLTMPHYERLGLDTTAVLCNGADLVNLSGTGPDPQTRYLSINKLDRVLSLFPTGIID
                     NLGLERDDDLYIYGDMSESPYHMELIKQSNTHDIDIHDIPRMDVGRVFLRIRDIPEAT
                     EIIGDLAKTEGISFYYRKVAGKRKQLYISIQSLLANKWFGIAEAAEYYGIARENIITF
                     GDEFNDEMMLEEAGLGFVMKNGNEELKKQVGNITRYTNVEGGVGKELIRLFNL"
     gene            complement(10189..>11082)
                     /locus_tag="VFD89_05040"
     CDS             complement(10189..>11082)
                     /locus_tag="VFD89_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ57589.1"
                     /translation="YDSYEDMLADENVDIILIATPNDVHKEQAIAAMRAGKHVLCEKP
                     VTPTSAELEEILAVSKETGMVFYPRQNRRWDKDYRIIKKIYDEKLLGDTFNIECRVQG
                     SRGIPGDWRGMKEYGGGMMLDWGVHLLDRMLIMIPEKIKKVFCSLTNITNEEVDDGFK
                     MHITFESGITSVIEVGTCNFLSLPLWYIAGIDGTAQIDNWDCEGQMARLTSWEDKDAT
                     PILAGAGLTKTMAPRKGNSVEFLPLPEVDFDNNELYSNLVGTINGTEEQIVTGEEALR
                     VLKLMEAAFESSEKGIVVNFE"
BASE COUNT         3025 a         2684 c         2072 g         3301 t
ORIGIN      
        1 gccctataga gttcttggcc ccataactct tcatcgggtt tttcgccctg ggcagttatc
       61 acattcactc cgcctaatag atcttcttta aacgtagcct ctagttttga atcctcaggc
      121 aataccaatg catgaaggtt aggtccatta tccacttcct ctaagcaata tacaatcggg
      181 ccatactgca cggcaacctt acctacattc gccctaacca tgggatttgc cctcatcctc
      241 acaggttcca tgggaaggac aagctcaatt ttatccccat cttcccatct acgtctaatc
      301 gtcgcatagc cgtctactac gatatcagct agatttatcg cctctccgtt tatggatata
      361 tagggattct gacaccaccc aggaattcga agtgctaaat caaaatcctt ggatccgtcc
      421 aatcccaccc ttatatcgat tcttccatcc cagggataat cagtagtctg atctatccta
      481 accttatcgc cgcctaccgt catttccagg ctactgccca tatataggtg ggcatatatt
      541 gtattatcat taaccctata tacataatgt ccaagggaag ccaataatct ggcgacattg
      601 gggggacaac atgcgcatcc gaaccatttt tgacgcacag gttttacatg tttcatattt
      661 ccattttttt cgcaggcctc aggatccact tccaaaggat ttacatagaa aaactgcttg
      721 ccatccaaag acatcccgct aatgacgccg ttgtatagcg ccctttccat tatgtctcca
      781 tattccccct tgggctcggt atctaacatt ctctttgcaa aaaatataag tcctattgcc
      841 gcacatgtct cggcatacgc cgtatcgtta ggtaagtcgt agtcaaacga aaacgcctcg
      901 ccgtgaacct gcgacccgat gcctccggta atgtacatcc tctttgatac agtgttttcc
      961 caaagcgtcc tacatgcctc cataagctct tgatcccctg tctccattgc aacatccacc
     1021 atagacgcat acatataaac cgccctcacc gaatgaccta cagcttctgt ctgctgtcgt
     1081 atcggcaggt gagcctggta gtattccttg cctttaccgg gcgcctcgct attatcccct
     1141 gtccaatatg atgttttgcc cctttttttc cattcgatat caaagaagaa gggctttcgt
     1201 ccccgctcgt ctaaaaaata ctttgcaagg tcgaggtatt tttcgttttc cgtgaccttg
     1261 taaagtttta caagggccaa ttcaatctcc tggtgcccgg gatagccttt aatcttatcc
     1321 gcctcaactc caaataccgt atcgatataa tcggcaaact tacacaccac atccaagagt
     1381 cttcgttttc ctgttacctt gtaatatgcg acagccgctt ctatcatgtg cccggcacag
     1441 tatagttcat gacattcatg aaggtttgac catctatcat caagaccctt gatggagtaa
     1501 taggtgttta gataaccatc ctcttcttga gcccttccaa ttaaatcgat aacctcatcg
     1561 gctatatcct caagtttggg atccggaaag gcttccagtc tataggcaac ggcttcaagc
     1621 cactttgcaa catcactgtc ttgaaagacc attccataaa aatctccctc ttcctcaccg
     1681 gcggcaattc gaaagtttct gattgccccg ctgggatctg catcggcaat cctgtcattt
     1741 aatgcttccc attggtaagg gatgaccgta tcgcgcacca atgctatata cctggaccaa
     1801 aaatcgtcgt tgatgctaac ttttaaatcc aacatgtcct caactcctta aaatatcggt
     1861 agcaattgca acacacaggt ttcaacgggg cgggtggttt atacccgtcg cctgtttgac
     1921 taatgggaac ccgccgctta tagaagcggg agacgtccta ccgactcgct atagaattaa
     1981 tgcagtgtag atatgcttgt ataatatata cccaatttta ccagctatcc ataaccgggt
     2041 caacttctta ggcccccaaa tttggtttat tcattcctat tcaattgatc ttggccagga
     2101 cctttagatt tttgttgtcg gggtttgttt gcaccttttt atgatttacc ctaatcccat
     2161 gatctaggtc tatccctatc ctactacggg ccagttcctt cagttttgtg tcaaaatcca
     2221 tgcactcttg tatctgcctt gatatctttt gataacgttt cttgacttgg gctatatttt
     2281 ccccatcctc ataatatccc gtatcctctt ttaggctgtt caagacatcc ctatagaccc
     2341 ctatcatctt atgaagataa tttatacgca ccctttctgt aatattccga tcatatctat
     2401 ggatatagat aagggcctta aaaccatttt gtctaccgct atcatagagc caatatatgg
     2461 gtcttttttg atatgctctt acatgatccc tataaaaatc ctttaaaaaa tactccctaa
     2521 ttatccttcg gggtgtgccg ctacccccca gaccgccggc tataaacccg agattatttt
     2581 ccaaggtccc ctcgccaaag gatactctta taaactcaat aaacttatct gtaatatctt
     2641 ctatatgata gtcatcatcc gtgatgatta aaatattatc cccagagggc ttaaatcttt
     2701 tatacttatt aacatcccat tcgccgcctg caaaggtcag ccccccaaaa tctaagcaat
     2761 agcggcccag catacatcct accccatagg aaataaacga tctaacatcc ctagctatat
     2821 ccgcatccct taaggttata tcctttgtat ctaaattggg tgatagctta tcatttaaac
     2881 catagagctt tataaatatc ccgtttaact tctcttcatt tcttttaagc ctttcaatcc
     2941 tgtctttggc taccctagcc caagcattga aggcgtcttc tatggtagct aacccatccc
     3001 gggacaaaaa tggatgcacg ctaaagcccc aggcttcttc gtaggagttc cattcctctc
     3061 tggctataga gatattctct tccaccagct ttataaccct ctcctcatgg gcaaatagca
     3121 ccggaaagtc agagaaatta cccacctgta ggttaattgt gggatttagc atcttaaaga
     3181 tatagttcga tacctttgag cccataagcc ctaacatata caaaagcctg cggtgatccc
     3241 cggtgaaggc agacatcccc gaaacatcat ggatgctccc agcctcccta tatctaatac
     3301 taaacccgcc gctggtaacc agtgaccatg taatggactc tttgaaataa taatccgggt
     3361 ttctaattgc cgatcgcctt ttcccattgc tgtctacaaa gcctcgtatt tcacatccat
     3421 cattttccca gttaactaca taatcgtaat tgccgtacca ttgccttctc attccacctt
     3481 tattataagg aaaccactta gccttcgaat ccatagatgc ctctgctgat ttggtggaaa
     3541 agcttatctt ccctatatct acctcatgcc atagccttaa aaacctatcg ttgttcccgg
     3601 tttgaagtcc tactttagct tcgattaagc tgcctgcggg ttctcctttt aaaaaggctc
     3661 ttattatatt ctcccctgcc caatatgcta tgggcattga gggtatcttt tcaaagtcat
     3721 tctgatgaat ttcataataa tggagtgatt gggtgtttcg tatggcctct aaggccttaa
     3781 gtctttgcac ttccatccct ccggggaaat ccgaaagctt aatatatttg cccttgccca
     3841 ttgccatatt attttgaact acaaaggtac acaatggcac tgtagcatcg ccaaaggcgg
     3901 aatattccat ttgaataagg catactatgc ttttagtccg gattatatgc tccctgagtc
     3961 tttcatggct ccttataaac atccatacaa aaggggacat ataggccgaa tagccgcctt
     4021 tcttgctcat cttcagatta ttatagatga agacagaaaa caggtctccc ttataatccc
     4081 tgtagttatt ccatatgtaa tcctttagcc tcttatcagg cttgtttaga taagggggat
     4141 tggtcaccat tatatggtac ttccccgtaa gcatggaaga tattttgacc agctttctaa
     4201 gttgattttg ggtctttctt atacccgatt ctcttattac agtttcacta cttcctgcat
     4261 gatctctatc acctgtacga tccctattac ctgtatgatc gctatcacct gtacgatcgc
     4321 tatcacctgt acgatctcta ttacctatat gaacactatc ccctgtataa tcactattct
     4381 ctatataatc actattctct gcataatctt ggatcaattc caataaagga tctgtgtgga
     4441 ttttatctat gcgcaatata gagcctattt ccgtagcatt tttaaacttt cctatcaaat
     4501 actttatttt ctctttggct atttcccttc tatccttatc catattaagg cctaaatggt
     4561 ctagaacttc cattttaatg ccggatgaat cctcaaagta tgtaaggtta tcctttaggt
     4621 cagaatacag tatcccatcg ttatatcgtc ttgccttcat tagtagtgta aaacgtgcaa
     4681 ggccgcaggc cctcctatct atatccagac catagagatt tttttctaaa ataagcctcg
     4741 atgcctcccg ggcggtatac ccttgggact tatagatcat aagcaataca tcaaaggcgt
     4801 agctgagaca atgcccactg cccatggcgt tatcgaatac ttttatttcc ccaggtaaaa
     4861 tctgctcctt cacaacggga atatctccgg gcatcaggta atctaagaaa ttcttgatgg
     4921 gactatgggg atttctctct aaccaatacc tgccgaggga attgtccacc atatatctga
     4981 caatccaatc tggggtaaat acttgagtgg ccgctgggat atcccgcttt cttatagtag
     5041 ttttagataa aacatcgaca accctgtccc ttggcccggt attataatat tgatataacc
     5101 agccgactac ctccacacct cccctcttct cccaagattc tatatcaaaa tgctcttctt
     5161 taatttggtg caccaattta tatatcacac ttgaaggatc gctataggaa atgatgggca
     5221 aaagctcggc gtatttgtca actttttcaa atagtatggg aaaatattga ttcatcgctt
     5281 ggcatagctt tataaataac aggcgaaacg ccctgtctgt cgatatgggg cttgcctctt
     5341 ttttccacgc ctctaattgc ctaatctctg tatccgtaag atcaaggccg gcatcttggt
     5401 atcggtccac caattccgga ttgtttaacc cccgcatttt cgatgaaaga gcgcgaatgc
     5461 caccaggaag ataaccattg acctccatga atcgaataac aataaaccta ataaaccatg
     5521 tacaggctgc atcctccacc aattctttat aggcggtttg atgattggga tatcttttcc
     5581 tatcatcaag ttcctttaaa agtgccctgt attgcccgat cccatctcct gatattataa
     5641 acggctctac tgagtcggtt ttaaatacca agacttcatc atgggatttc attttattta
     5701 gcggcagttg tatcccatcc tctgtgagcc ccatcatgga cgccctgtcc tttataccct
     5761 ctattaactt ttcccttgca tacattgcaa agcttcttaa agctgtgtta gtcatgacat
     5821 cctcctaaat ccatagctga tcctattaaa acatcggaaa caattgcatt ggcaattgca
     5881 acacacaggt ttcaacgagg cgagggattt atacccaccg cctgttttac tttactaatc
     5941 aaaacttgct ccctatagaa gcggggggac atcttatcgc ctcgttataa cggctattta
     6001 atataacgat ttttgcagcc cacactaaaa gggtttaatt tttcttgcag taaagcgcag
     6061 ttggtgtggt agagtcatgg atggaattgt cccggacgag ataaccactc ttgaaatact
     6121 ctatggcagt ggtatataat ggtcataacc acaagcttat agatcaataa ctccaaccat
     6181 agaatgatta tagcccaaga gtttagcctg ctcaacctat ttagatattt cataaagttg
     6241 tcctatcaat ttatgtttaa ataatatata gggggaatat atatgtgtga taccatggta
     6301 gccttaggca attccaccag ggacggtgtg accatctttg ctaaaaacag cgatcgtcaa
     6361 cccaacgaac cccacatcat gatcagggtg cctgccgcag atcatgagga aaactccaag
     6421 gtcaaatgca cctatataga ggttgaccaa gtcccacata cctatgagat tttactgcta
     6481 aagccctctt ggctttgggg ctgcgaaatg ggatgcaatg aatttggctt aaatatcgga
     6541 aatgaggcgg tatttaccaa agaaccctat ggggaagatg ccttaacggg catggatatg
     6601 atacgtttgg ctctggaaag atgcaaaacc gccaaagagg cccttatgct tattataggg
     6661 ctcctggaag aatacggtca gggcggtaac tgtggatacg aaaaggactt cacctatcat
     6721 aattcctttc tcatagccga tagcgattcc gcttgggttt tagagaccgc cggaatttat
     6781 tgggtcgctg agaaggtaaa ggatgttcga agtatctcca attgcctatc catcggcagt
     6841 gagtttgacc tccatcatcc ggatctgata aagaacgctg tagataaggg atggtgtaag
     6901 agcgaggacg attttaactt tgctaaatgt tataccgaac ccatagttac ccactttagc
     6961 ggttctaaag ccaggctagc caccagtact tcaattctaa agcagaacaa aggacagatc
     7021 accgtagata ccatgaagaa tatcctccgc tcccatgtgg gtcgggtaga cgcaaatccc
     7081 tttaaaaaga gttccctcaa aagcgtatgc atgcatggcg gctttatctt tggcgaccat
     7141 gcaacgggca gctatgtagc gtctatccaa gacggactac ccacctattg gataaccggt
     7201 agttctaccc cctgcatatc actttttaaa ccctattgga tgctggacga ggaagaattc
     7261 ctcttcgaag aagatgaaga ggacaaggct ctagagtatt ggcatgtgag ggaagaattt
     7321 catcgtatgg taatagaaaa tagaatccaa gaccttgatc gttatataaa gaagcgggat
     7381 gagatagaac gggatctatc ccatatggtt ggtgcccttg atatggaaaa tccaaatacg
     7441 ggcgtgttaa gggatataat ggataaggct tttagggagg aagaagaact cattttagat
     7501 tttctagccc gaaatcggga caatccagta aggattaaag gcaatccata ctttagacgc
     7561 tactggcgca aacaaaacga gaaactgcgc acccaaacgc ctatgaccta atgggaaatc
     7621 aaattaataa ttaatataaa gtaatgatat actcagtggg agggggacaa tcacaaacac
     7681 aggtatatat atcttgagtc ttcattttaa tatatagaca aacaacttca actataaaat
     7741 ttaccgccta cagataattt ttgtttccgt attgtttatc tattatccat ttctttaaag
     7801 aaatggaatg gtgaagacga agttcaacat aatccaaacc aaggacaaat attcatattg
     7861 aggaggaagt ccatgtctaa aaaaactatt attggagaac cgcttcccaa cattccatgg
     7921 gaagaccgac ctgaaggatg taatgatgta atgtggcgct atagcggaaa cccggtgatc
     7981 ccaagggatc ttatacccag ctccaacagt attttcaaca gcgctgtcgt cccctttgag
     8041 ggggagttcg ccggagtatt caggtgcgac gataagagaa gggtaatgca aattcacacc
     8101 ggcaggagca aggatgccat caattgggaa ttagaaaatg accctatatc ctttatctgt
     8161 gacgatcctg agataggcaa tttcgtttat ggatatgatc cccgggtctg taagattgat
     8221 gaccgctatt atgtaacgtg gtgtaatggc tatcatggtc ccaccattgg agtggcatat
     8281 acatacgact ttaaagagta ctatcaactg gaaaatgcct ttttaccttt caacagaaat
     8341 ggtgtcatgt tccctaggaa gataaatggg aactttgcca tgctcagcag gcccagcgat
     8401 aacgggcata cccctttcgg agacatcttc tacagtgaaa gccctgatat gacatactgg
     8461 ggtaagcata ggcatgttat ggctaccagt gagggttggc aggccacaaa aatcggtgca
     8521 ggccctattc ccattgaaac caccgaaggt tggctcatga tctaccacgg cgttttaact
     8581 tcatgtaacg gattcgtcta cagcgtcggt gcggctctat tggatattga cgagccctgg
     8641 aaggtactct atagaacaga accttatata ctctcccccc aaaccctata cgagtgcgtg
     8701 ggcgatgtgc ccaacgtagc attcccctgc gcagctttag ccgattcgga aaccggtcgt
     8761 atagcaatgt attatggcgg agcggatacc gttacctgtg tagcatttac ccaggttgac
     8821 cttctcattg actatattaa gaataattcc aaggtctaat atagcgagac caatgtggcc
     8881 atccccttat caatggctca ttgcctttgt cagatattaa catgattaac cggtacgaca
     8941 ggttaatcta actaatatta aaaatgtgtc cggttggatt aatgtaatcc ggccgggcac
     9001 atttgaagtt atagtcaggg ctgggcagta tagacccggt gacctgtttt ttactaccct
     9061 tgttaaaggt taaaaagtct tataagctcc ttacccactc caccctcaac gttggtatag
     9121 cgcgtgatat ttcccacctg cttttttaat tcctcgttgc cgtttttcat cacaaaaccc
     9181 agaccggcct cttccagcat catttcatcg ttgaattcat ccccaaaggt aatgatattt
     9241 tcccttgcta tcccataata ctctgcggcc tccgctatac caaaccattt attggccagt
     9301 agggattgta tgcttatata gagctgtttt ctcttacccg ctaccttcct ataatagaag
     9361 ctgatgcctt ccgttttcgc tagatcaccg ataatctcgg ttgcctcggg aatatccctt
     9421 attctaagaa agacccgacc aacatccatc ctgggaatgt catgtatatc aatgtcgtga
     9481 gtattcgatt gctttataag ctccatatgg tacggtgatt cactcatatc accatatata
     9541 tatagatcat catccctctc aaggcctaaa ttatcgataa tacccgttgg gaataggctt
     9601 aagaccctat ccaatttatt tatggacaag taccttgttt gcggatcggg cccggtgccg
     9661 gacaggttaa ccaaatccgc accgttacac agtacggctg tggtatctag ccctaacctt
     9721 tcatagtgcg gcatggtcag gcaagatggc ctcgctgtgg caattattac cctatggccc
     9781 agcgctttta gtttctttaa cgcctccgca gtcacatcgt ttagctcata tagattgtcc
     9841 aaagcagtat gatctaaatc tactgctatt aaatgcattt tacccaataa tggatcctcc
     9901 tagtataatt ggaaacccct gaaatacgca aaggattttc aggggtttcg tacaattgaa
     9961 gttcatataa aaccgcagat aaacaacttc agctttaaaa tttaccgcct acagataatt
    10021 tttgtttctg tattgtttat gttagataaa caacttcagt tttaaaattt ccccttcatg
    10081 aaattgcata gaaccgtagc aattatcatt acggtaaatt tagttgacct aatgttaatc
    10141 tatatattag gccgtttaat aagtttgaac taaagatgca ttaacccttt attcaaagtt
    10201 tacaacgatg cctttttcgg aggactcaaa ggcggcttcc atgagcttga gcacacgaag
    10261 tgcctcttcc ccggtaacta tttgctcttc agtaccgtta atggtaccca ctaaattact
    10321 atataactcg ttattgtcga aatctacctc gggcagtggt aaaaattcca ctgaattacc
    10381 cttgcgtggc gccatggtct tagtaagacc tgcaccggcg agtatcgggg tagcatcttt
    10441 gtcttcccag cttgtcaggc gggccatttg tccttcacaa tcccaattat caatctgtgc
    10501 ggtgccatca ataccggcaa tgtaccacag gggcagggaa aggaaattgc atgttcctac
    10561 ctcgataacc gaagttattc cgctctcgaa ggttatatgc attttaaagc cgtcgtctac
    10621 ctcttcattg gtaatattgg ttagggagca aaagaccttt ttaatcttct cgggtatcat
    10681 gatcaacata cgatccagta gatgaacgcc ccagtccaac atcatcccgc caccgtattc
    10741 cttcataccc cgccaatcgc caggtatacc acgggaaccc tgtacgcggc attctatatt
    10801 aaaggtatca cctaacaact tttcatcata tatcttcttg attatgcgat agtccttatc
    10861 ccatctacga ttctgtctgg gataaaacac catacccgtc tccttggata cagcaagtat
    10921 ctcctctaat tctgcgctgg tgggcgtaac aggcttttca cacaatacat gctttccggc
    10981 acgcatagcg gctatggcct gttccttatg aacgtcattg ggagtagcta tcagaataat
    11041 atccacgttc tcatccgcta gcatatcctc atagctgtca ta
//
LOCUS       DASRNX010000124         3658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_448814,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000124 DASRNX010000000
VERSION     DASRNX010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448814"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFD89_05045"
     CDS             <1..410
                     /locus_tag="VFD89_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HZJ57590.1"
                     /translation="LDICGIEYDKNSYSGKSLVPLIEGDPNATANWKDEMYTQTNGNE
                     LYGIQRSVMTPKWKYVYNGFDYDELYDLEEDPHELVNVANNPVNRPIIRKLAAKLWKF
                     AYDRKDTCINPYIMVSLAGFGPGICMEGCQGGV"
     gene            557..796
                     /locus_tag="VFD89_05050"
     CDS             557..796
                     /locus_tag="VFD89_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57591.1"
                     /translation="MKNTLIKLGLVAIVIIAVIGATFVLSGKKNPTDYIIDRRDSEYK
                     MGEIGGNSGYKSCNVFHYNNKWNNDGTSNESDPGQ"
     gene            complement(1048..2712)
                     /locus_tag="VFD89_05055"
     CDS             complement(1048..2712)
                     /locus_tag="VFD89_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57592.1"
                     /translation="MSYKRFMCLLVVILLLAGIIAGCGTGGKPTKTDPVDSGEKEVVD
                     KDEDKDKDKDKVDEPSPPERDIIKVSMNVMDAEKNGNNPKTDFILEEFGIEFEYWPVN
                     WGDWNEKINNWISVNDTPDLIWWDLKGTQSNQYRTWARQGAFSPIPYDMIAKYPNLQD
                     RVDTMESFKHLEVDGDLYGWASSRRYDPMVKNTYSSVWLYRRDWAKEVGLFNEDHVYE
                     WEEWKNLIKTVIEKDPGGNGAGNTAGMVMPPWAFPHAPVLFVGSVPAEGNETCSYIKV
                     DDEWVWPPMLDSYKEEVAETWKMYQDGLIWKDNMGFSGSEDEELFKGGRAFARYQSGV
                     GVLNNFSKDMLEDGIIEKDSDMGPAIVKDRNGDVWLTQTEDFWTVTAFSNKVDEEKMD
                     RILEFWDYLNSEEGLLFIEMGFEGKDYEIDANGEVKVLWEVDPATERYVSPYSDFAFG
                     TLTPATLVPEINHTSMPFGINEHLAMYEKLNELNFKTKPFNYEMSFYSAPEKDKYGNF
                     GADAKTKLSEIIADSNTDPTAEWEAWVKSMMPRVQPILDELNEGLK"
BASE COUNT         1080 a          782 c          752 g         1044 t
ORIGIN      
        1 ttctggacat ttgcggaatt gaatacgata aaaacagcta ttcgggcaag agtctcgtcc
       61 cccttatcga aggggaccca aatgccactg caaattggaa ggacgagatg tatactcaaa
      121 ctaatgggaa tgagctatat gggatacagc gttccgtcat gacgcctaag tggaagtatg
      181 tttacaacgg ctttgattat gacgaattat atgaccttga agaagaccct catgagcttg
      241 taaatgtagc caataatcct gtgaatcgtc ccataattcg caagcttgcg gcaaagctgt
      301 ggaagtttgc atatgacagg aaagatacat gtatcaaccc ttatataatg gtatccctcg
      361 ctgggttcgg gccgggcata tgtatggaag gctgccaagg aggcgtttag tctgaaacca
      421 aaaacgaact ggcagtccca ccttacccca gagagtatta agccatacca aggtgcttat
      481 ataataaccc cagaaacgtc tgcataaaat ttataattgg cacgggaatt acccaatagt
      541 gattggaggt tatgatatga aaaatacact tataaaacta ggtttggtgg ctatcgtaat
      601 aatagcagtt attggagcaa catttgtgct gtcgggaaag aaaaatccaa cggattatat
      661 tatcgatagg agagatagcg agtataaaat gggggagatt ggcggtaaca gcggttataa
      721 aagctgtaac gtattccatt ataataataa atggaataac gatgggacat ctaatgaatc
      781 ggatccgggc caataaaggg cccatgccct aaaaatgaat gggtgatgag aatgcgtaaa
      841 aatcattcga taggaactac accctgtatg ccttcaatac taatccctac aaacttccga
      901 tatatggtcg cccagatttt gcacggcggt acaaaactat attataaccc tttggcagtc
      961 gggtcactaa acaaacagac gcaaagctta cagatataca aaaaaacctg gtagaattac
     1021 taccaggttt ttagcggtcc ataaaaatta tttcaggcct tcattcaatt catccagtat
     1081 aggttgaacc cttggcatca tagatttaac ccatgcttcc cattctgcag tgggatccgt
     1141 attcgaatcg gctattatct ccgatagttt cgtttttgca tctgcaccga aattaccgta
     1201 tttatccttc tcgggggcag aatagaagga catttcataa ttaaatggtt tggttttaaa
     1261 gttgagctcg tttagttttt cgtacattgc cagatgctca ttgataccaa acggcatgga
     1321 agtatggttt atttcgggca ctagggtggc cggtgttaat gtaccaaaag cgaaatccga
     1381 atatggactt acataacgct cagtagcggg gtctacttcc cataaaacct ttacttcacc
     1441 attggcatca atttcatagt ccttaccctc aaatcccatc tctataaaaa gtaatccctc
     1501 ttcagaattc aggtaatccc agaattccag tatgcgatcc atcttttcct cgtccacttt
     1561 atttgaaaag gcggtaactg tccaaaagtc ttcggtctga gttaaccaaa catcaccgtt
     1621 acgatcctta acaatggccg gccccatatc cgaatccttt tcaataattc cgtcctctag
     1681 catatccttg ctgaagttat tcagtacgcc cacgccggat tgatacctgg caaaagcacg
     1741 cccgccttta aacagctctt cgtcttcact tccgctaaag cccatgttgt ccttccaaat
     1801 caggccgtct tgatacatct tccaagtctc tgcgacctct tccttgtagc tgtccaacat
     1861 cggaggccat acccattcgt catctacctt gatatatgag catgtttcat ttccttctgc
     1921 gggaactgat cctacaaaga gtaccggtgc atgggggaaa gcccaagggg gcattaccat
     1981 accggcagtg ttgccggctc cattgccgcc cggatccttt tctataacag ttttaattag
     2041 gttcttccat tcttcccatt cataaacgtg atcttcattg aaaagaccta cctctttagc
     2101 ccaatctctt cggtagagcc agactgatga ataggtgttt ttcaccatcg gatcgtacct
     2161 tcgcgaagac gcccaaccgt ataagtcacc gtcgacttca agatgcttga aggactccat
     2221 ggtatcaacc ctatcctgga ggttggggta ctttgctatc atatcatagg ggatggggct
     2281 aaaggcacct tgccttgccc aggttctgta ctggttggat tgagtcccct ttaaatccca
     2341 ccaaataagg tccggtgtat cgttaacgct tatccagtta tttatcttct cattccaatc
     2401 tccccagttt acaggccaat attcgaactc gatgccaaac tcctccaaaa taaaatccgt
     2461 cttagggtta ttaccgtttt tttcagcatc cattacgttc atggagacct tgataatatc
     2521 ccgctcgggg ggtgagggtt catccacctt gtccttgtcc ttatctttat cctcatcctt
     2581 atctacaact tctttttccc cggaatctac gggatcagtc ttggtaggtt ttccacctgt
     2641 accgcagccc gcgatgatac ccgccagcaa tagaatcacg accaataagc acatgaatct
     2701 tttgtacgac ataattatga gcaccccttt catttatctt tatcgctgtg gataatgata
     2761 tattgcaaat ccggtgctgg gcaccgggct ttacacaaag atttggtcat tcctttacta
     2821 aatctggtga taaatcggaa agaagcatcg ttgcagacaa tgaggcatcc gaagggtgat
     2881 aaatccgaaa gaaacatcgc tgtagataag aaggtatcta tcttggaatt gtctatctat
     2941 atgcggatac taatttaaaa atgaatagga tagatagctt gctaattatt ctgtggtaca
     3001 attatacaca ttttaaccaa atatttcaag tccaaaagtt ctcttgtgaa gccgaataat
     3061 tggggtgttt aacactttta cgcatatcct aaagatccat cgttcagctg taggcagatg
     3121 tcccatgtaa tgtccaaatt attaaataag gccctagatt aatagtttta tctttctagg
     3181 caacgtatca aaatctaaga tatgtggtgc cggcaatcgt ccccaaacat gatgaaaggt
     3241 cacgatttca atcatggata agagagccat actttccaat tatatgttgt agtatacgat
     3301 aaaaatgtat ataatgggtt atgaaatggg aatacaggag gattaaaaca agggatcatt
     3361 aatcaactac aattttgttt ttatccaagg catggacgaa agaaaatttt tagacactaa
     3421 gggggaatag ctttttcatg ggattgttca ggtgaactgc ggcaccaaac cgggatttaa
     3481 aaaactggtc gtgggacgta ttctaaaggc atgctgaaag tagacagacg tatttttaag
     3541 gtaacttagg ggtagagcgt gtcataaagg cgtacaagag gtagggagta ttataaagat
     3601 gagcaaaaag ccatacatat tttaaaggcg ggctaggaaa tgataagtga aaatgggg
//
LOCUS       DASRNX010000125         7004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_395742,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000125 DASRNX010000000
VERSION     DASRNX010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395742"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1916)
                     /locus_tag="VFD89_05060"
     CDS             complement(<1..1916)
                     /locus_tag="VFD89_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HZJ57593.1"
                     /translation="MLFRSFLDIECKELVRGWTLKKTKVYLKDRLWEIIIQTDDYVDA
                     PTLSKIGKSIQDRIGNITDVRIIVERTEGPEWFYDNFDVIWADIVYDANKDFPDCARW
                     LSHCRTRLKGYGLELAFNNPMILQLLDTKGIDRYIETWIDDRYSLRFKVKLKVDGDSL
                     EARQTEYNAKKRLEDSGFIKDAIEAANQVKSVKKVDGGPNRGHILFGKLVKGEPIAID
                     MLREDSGRVIVKGKVFDIESRETRSGKIILIMDITDYSGSITIKTFCDKDQGDRIGER
                     IAKGDWVMARGECSYDKYQRDISIIFDDLMLLQSVDRTDDEPFKRVELHLHTQMSAMD
                     GVSTVDSLVSRAAKWGHQAIAITDHGVLQAFPHAYAAGKKYGIKIIYGMEAYLINDCK
                     PMILNGNGLDFNQEIVVLDIETTGLDAKNDAITEIGAVKILDREIVDTFQTFVNPGMA
                     IPQKITRLTGIDDNMVKDAPMIDEVLENLKDFCGESALAAHNAPFDFGFINEKAKVLD
                     WKIKNPIIDTLSLSRELIQDLKRHKLNHVAKYFGILLDKHHRAYHDAMATGEILIKLF
                     DILEGKGVRTLSEINATFTRITNYSSLPATHALVLVKNMKGLNNLYKLVSKSHMDYFY
                     RQPRIPKSIMSQYR"
     gene            complement(1942..2571)
                     /locus_tag="VFD89_05065"
     CDS             complement(1942..2571)
                     /locus_tag="VFD89_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZJ57594.1"
                     /translation="MKVSAIIPAYNEEKNIGMVLSILTQIEEISEILVINDGSVDKTS
                     EIARMYGVEVVEFTENHGKSLAMWAGVTRTNAPVVIFLDADLIGLTRQQVKDMILPIT
                     NGYADMTLGIFCQGRSMTDLAQRIAPYLTGQRAMKRWVLDSLDKEDWQTGFGIEMTIT
                     RLAKTSNIRIMEIPLENTTHAMKEEKMGFVKGVAARARMYWEIIKQLNY"
     gene            complement(2771..3805)
                     /locus_tag="VFD89_05070"
     CDS             complement(2771..3805)
                     /locus_tag="VFD89_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HZJ57595.1"
                     /translation="MTTIITALILFGIIVLAHEMGHFLVASWVGIPAEEFSIGMGPRI
                     SQLKGKKTIFSLRALPIGGYVKFIGDDEESDDPRAFGNAKVWKRILVIAAGPIMNFLL
                     AVLLLTLFFTVFGVYNNSTHIFEIVEGSPAEHAGLQVDDKIIEIHDVKVDLDDQEKGI
                     ELFRSILQDKGDQPLKLTVIRDGETREIDIKPDYSDDKGTYEIGIYFGNLVKLGPFSA
                     IGLAFVQTGRLIIMMLQLLGSLIFKGRGLNEVVGPVGIIGEIGKAVKSGVKDVINFAV
                     VITLNLGIMNLIPFPALDGGRLALLLVEGLRGNPIDPKKEGYIHFVGFIILILLMIVV
                     TFKDVTRQWF"
     gene            complement(3914..5068)
                     /locus_tag="VFD89_05075"
     CDS             complement(3914..5068)
                     /locus_tag="VFD89_05075"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZJ57596.1"
                     /translation="MVRRIAIVGSTGSIGTQALDIMEDYPEQFEVKALAARQNIDLLQ
                     LQIEKYQPKTVVITDGRRARELSQRLPLRGTEIKSGMEAITELVTDEEIDVVIMAIVG
                     IAGLEPTIAALKAGKTVALANKEVMVVGGHIINRLIRQYHGRIIPVDSEHSAVFQAIQ
                     GCKDPDEVNRIILTASGGPFRGYSVDMLKDITPKDALRHPNWEMGDKITIDSATLMNK
                     GLEVIEAKWMFNVDLDRIEVVIHPQSIIHSMVEFIDGTIIAQMGIPDMRIPILYALTH
                     PYRNRTGLEPLDLINMGNLTFEEPDLTTFPCLSLAYEAMEIGGTMPVVLNAANEVAVD
                     LFLKERLAFYQIPIIIEGAMSSHKIIGDPQIDDILSVDGEIRSELESRYR"
     gene            complement(5216..6025)
                     /locus_tag="VFD89_05080"
     CDS             complement(5216..6025)
                     /locus_tag="VFD89_05080"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZJ57597.1"
                     /translation="MLTQRIVSAAIGIIYIFLMLHLGGWFFNISVLIMALILMHEFYH
                     AFIKKGYRPLTWLGYSFVIILFYFCIISYKYTVVVLVSIFILTGLTMPVFFKRVTIMD
                     VLTTMFAVLYPGMMIFFLILLAFKSPPYGMNLLILGFLITWSTDTFAYFLGKTFGRKQ
                     LCPLVSPNKTVEGGLGGLVGAMLMGALFGYILNHSFGFEIPLYHFGLMGFVGSIFSQL
                     GDLSASSIKRFCGVKDFGKILPGHGGLLDRFDSLLFSLPIIYIYYLVFLAP"
     gene            complement(6028..6828)
                     /locus_tag="VFD89_05085"
     CDS             complement(6028..6828)
                     /locus_tag="VFD89_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HZJ57598.1"
                     /translation="MNFYKKIKQRKALIKHNRVTMDSVKSKPIPRHISIIMDGNGRWA
                     EKKGLPRIAGHRQGVEKMREIITTSAELGVKYLTLYAFSTENWKRPKGEIKAIMSLLV
                     EYLRRELNELHKNNIKIMTIGDTTSLPDIAYEEIQKAINITSANTGLQINVALNYGGR
                     MEIVLALREISRKIANNELDIDDIDEELISDHLYTGGIPDPDLLIRTGGDIRVSNFLL
                     YQIAYTELWFSDTTVYWPDFGVGHYLDAIHDFQNRQRRYGGVIGKGDD"
BASE COUNT         1978 a         1656 c         1221 g         2149 t
ORIGIN      
        1 tccctatact gtgacataat acttttgggt attctgggtt gcctatagaa ataatccata
       61 tgggacttcg agaccaactt ataaagatta ttcaagccct tcatgttttt gaccaataca
      121 agggcatgag tggcgggcaa ggagctatag tttgtaatac gggtaaatgt ggcgttaatc
      181 tctgataagg tcctaacccc cttaccctcc aaaatatcga atagttttat tagtatttca
      241 cctgtagcca tggcatcgtg ataagcacgg tgatgcttat ccaatagtat accgaaatat
      301 ttggcaacat gattcaattt gtgtcttttt aaatcctgaa ttaattccct tgaaagagaa
      361 agggtatcaa taatgggatt ttttattttc cagtctaata cctttgcctt ttcgttgata
      421 aagccaaagt caaatggggc attgtgagca gctagcgcac tctccccgca aaaatctttc
      481 agattttcaa gcacttcgtc tatcatggga gcatccttta ccatattatc atcgatcccg
      541 gttagtctgg taattttttg gggtatggcc atccccggat ttacgaaggt ctgaaaggta
      601 tcaacgatct ccctatctaa aatctttacg gcccctatct cggttatggc atcattttta
      661 gcatccaatc cggtggtctc gatatccaaa actactattt cctgattaaa atccagccca
      721 ttcccattta gtatcatggg tttgcagtca tttatcagat atgcttccat cccatagatt
      781 attttaatgc catacttttt acctgcagca taagcgtggg ggaaagcctg caacactccg
      841 tgatcggtga tagctatggc ctgatgtccc catttggcgg ctctggatac cagcgagtcc
      901 acagtggaca caccatccat ggcgctcatt tgggtatgca aatgcaattc tactctttta
      961 aaaggttcat cgtccgttct atccacggat tgcaagagca ttagatcatc aaatatgatg
     1021 gagatatccc tttgatattt atcgtatgaa cactcgccgc gggccatcac ccaatcaccc
     1081 ttggcaatcc tttcacctat cctgtcgcct tggtccttat cacaaaaagt cttaatggtt
     1141 atagagccgc tataatccgt tatgtccata ataagtatta ttttcccact tctggtttcc
     1201 ctggattcta tatcgaatac tttccctttt acaattactc tgccgctatc ttcccttagc
     1261 atatctattg ctatgggctc acccttaacg agtttaccaa aaagaatatg gcccctattt
     1321 gggcctccat caaccttctt taccgatttc acctggttgg ctgcctcaat ggcatcttta
     1381 ataaagccgg aatcctccag ccgctttttt gcattatatt ctgtttgtcg tgcttctaaa
     1441 ctgtccccat ccacctttaa tttgactttg aatcgaaggc tatatctatc atctatccat
     1501 gtttcgatat atctatcgat acctttagtg tctaacagtt gtaagatcat tggattgtta
     1561 aaagctagtt ccagaccata tccctttaat ctggtacggc aatgggatag ccatctagca
     1621 caatcgggga agtctttatt ggcatcgtag actatatcgg cccagattac gtcgaagttg
     1681 tcataaaacc attccggacc ttcggtgcgt tcaacgatga tcctcacgtc agtaatattt
     1741 cctattctat cctgtattga ttttcctatc ttggataagg taggggcatc aacataatca
     1801 tcggtttgaa taatgatctc ccacagccta tccttcaaat atactttggt tttctttaat
     1861 gtccaacccc gaaccaattc tttgcattct atgtctaaaa agctacgaaa aagcaactta
     1921 ttaagttccc cccaaccata gtcaataatt taattgtttg atgatttccc aatacattct
     1981 tgccctagca gccacgcctt taacaaagcc catcttctct tctttcattg catgggtagt
     2041 gttttctaat ggtatctcca taattcttat gttagaggtt ttggctagtc gggttatcgt
     2101 catttcgatc ccaaaccctg tttgccaatc ctctttgtcc aggctatcga gaacccatct
     2161 cttcatagcc ctttgccctg ttaagtaggg ggctattctt tgggccagat cagtcatgga
     2221 gcgtccttgg caaaagattc ccaaagtcat atcggcatac ccattagtaa ttggaagtat
     2281 catgtcctta acctgttgcc tagtcagccc gattaagtct gcatcaagaa aaataacaac
     2341 tggagcgttg gtacgtgtta cccccgccca catggccagg ctcttcccat gattctctgt
     2401 aaactctaca acctctacgc cgtacatacg agctatttca ctggttttat ctacagaacc
     2461 atcattaata accagaattt cagatatctc ctcaatctgt gttagtatgg aaagaaccat
     2521 accgatattc ttttcctcat tatatgctgg tattatcgca gatactttca tatcttgttt
     2581 tcccccggat cttaaaaaaa tggcccatta tctcttctgt ggatatatcc ctacagcgta
     2641 tgttttctta agcgaataat cccatcccaa aactcccgtt aaaatatcgg aagcaattgc
     2701 attggcaatt gcaacacgca ggtttcaacg aggcgagaga catcttgccg actcgtttta
     2761 atccttgcag ctaaaaccat tgtctggtaa cgtccttaaa agtaacgact atcataagta
     2821 aaattaggat tataaatccg acaaaatgga tatacccctc tttttttgga tcgatgggat
     2881 ttcccctgag accttccacc aataagagtg cgagccttcc cccgtctaag gcggggaagg
     2941 gtattaagtt cataatcccc aaattcaagg ttatcacaac agcaaagttt ataacatcct
     3001 ttacaccgga ttttacagcc ttacctattt cgcctatgat tcctaccggg cccaccactt
     3061 catttaatcc gcgaccttta aaaatcagac tccccagtaa ttggagcatc ataattataa
     3121 gcctgcccgt ctgaacaaaa gcaaggccaa tggccgaaaa aggccccaat tttaccaagt
     3181 tgccgaaata aatacctatc tcgtatgttc ctttgtcgtc agagtaatcc ggttttatat
     3241 ctatttcccg ggtttcaccg tctctaatga cagtcaactt taatggttga tcccccttgt
     3301 cctgtaagat acttctaaaa agctcaatac ccttttcctg gtcgtctaaa tcaaccttaa
     3361 catcgtgaat ttctataatt ttgtcatcca cctgtaagcc ggcatgctct gccggagatc
     3421 cttcaactat ctcgaatata tgggtggagt tattgtagac tccaaagact gtaaaaaata
     3481 gcgttagaag caaaactgcc aagagaaaat tcattatagg tccggctgca attaccagaa
     3541 tgcgtttcca aacctttgca tttccaaatg ctctgggatc atcgctctcc tcatcgtcac
     3601 cgataaattt tacatacccc ccgataggta gggcccttag ggaaaatata gttttttttc
     3661 cttttagttg ggatatccta ggccccatac cgatagagaa ttcctcggcg ggaattccta
     3721 cccaactcgc taccaaaaaa tgccccatct catgggccaa tactattatc ccaaacagta
     3781 tcagagccgt tataatcgtt gtcatcgtgc accttccttt ttatataggg atagaatttt
     3841 taaaacacta gggggaattt taataagata cccagcatag cgaaggttat acataccgtc
     3901 aatcgccccc ggttcagcga taacgacttt ctaattcact acgtatttcc ccatccacag
     3961 ataatatatc gtcaatttgt ggatctccaa ttattttatg ggatgacatt gcaccttcta
     4021 ttattattgg tatttggtaa aaagctaatc tctctttgag aaataaatct actgcaactt
     4081 cattagctgc attaagaacc accggcatag ttccacctat ttccattgct tcataagcaa
     4141 gggaaaggca tggaaatgtg gtcaaatcgg gttcttcgaa ggttaggttc cccatattga
     4201 taaggtctaa aggctctaat ccggttctgt tcctgtaagg gtgcgttaac gcatatagga
     4261 ttggtattcg catatcggga atccccattt gagctatgat agtgccgtct ataaattcca
     4321 ccatagaatg tattatgctc tggggatgta tcaccacttc tatcctatcc aaatcaacat
     4381 taaacatcca ttttgcttcg ataacttcca aacccttatt cattagggtc gcagaatcta
     4441 tggtgatctt atcccccatt tcccagttgg ggtgtctcag agcatctttt ggggttatat
     4501 cctttagcat atctacgctg tatcccctga agggtccccc agatgcggtt aggataatcc
     4561 tgttcacttc gtcgggatcc ttacatccct gtatggcttg aaaaacagct gaatgctcgc
     4621 tgtcgaccgg aataatccta ccgtgatatt gcctaatcaa acggtttatt atatgtccgc
     4681 ccactaccat tacttcctta ttggcaaggg caacggtctt acccgccttt aaggccgcaa
     4741 tagttggctc taaacccgca atacctacaa ttgccatgat tactacatca atttcctcat
     4801 ctgttaccaa ctctgttatt gcttccattc cggattttat ctctgtacct cgtaaaggca
     4861 acctttggga gagttccctt gccctacggc catcagttat aacgaccgtt tttggttggt
     4921 atttttcaat ctgcagttga agtaaatcga tattttggcg agctgccaag gccttgactt
     4981 caaattgctc cggataatcc tccataatat ccagggcctg tgtacctatg gaccccgtcg
     5041 atccaacaat ggctatacgt cttaccaaat aaatacatcc tcctaccgtc ttgcggtttt
     5101 attccaaaat cggctttaca gaatcttatg taaggtcgta tcgaaaataa gtccccgtgg
     5161 tatagaattt aaaagcatgg tccatttcat catttgatca tgggcaaagg atagattatg
     5221 gagccaaaaa gactagatag tatatataga taatcggcaa tgaaaatagc agactatcga
     5281 acctatccaa aagaccccca tgccccggaa gaatctttcc aaaatcttta acaccgcaaa
     5341 acctctttat agacgatgcg gacaagtctc ccagttggga aaatatactc cctacgaagc
     5401 ccattaatcc aaaatgatac aggggtattt cgaatccgaa ggaatggttt agtatatatc
     5461 cgaatagggc acccatcagc atagccccga ctaatccgcc cagaccaccc tctactgttt
     5521 tattagggct tactaaagga cagagttgct tccttccaaa agtctttccc aaaaaatatg
     5581 caaaagtatc cgtagaccaa gttataagaa aacccaaaat aagcaggttc ataccatagg
     5641 ggggcgattt aaaggccaac agtataagga aaaaaatcat catacccgga tataagacag
     5701 cgaacatcgt tgtcaataca tccattatgg taaccctttt aaagaatacg ggcatagtta
     5761 acccggtcaa aataaatata gataccaata ctactacagt atatttataa ctaataatac
     5821 aaaaataaaa caatattata acaaatgaat aacctaacca agtgaggggt ctgtatccct
     5881 ttttaatgaa cgcatggtaa aattcatgca ttaagatgag ggccataatt aaaaccgaaa
     5941 tattaaaaaa ccaaccccct aaatggagca tcaagaaaat atagataatc ccaatagcgg
     6001 cactaacaat tctttgagtc aacactttca gtcatcccct tttcctatca cacccccata
     6061 gcggcgttga cgattttgga aatcatgaat ggcatccaga taatgcccca caccaaagtc
     6121 aggccaatag acggtggtat cagaaaacca aagttcggtg tatgcaatct gatataataa
     6181 aaaattgctg acccttatat ccccaccggt tctaattagc aggtcggggt caggaatccc
     6241 gccggtgtag agatggtcgg aaattagctc ttcatcgata tcatcaatat ccaactcatt
     6301 attagctatt ttacgcgaga tttctctaag agctaataca atctccatcc ttcctccgta
     6361 gttaagcgca acattaattt gcaaaccggt attagcagaa gtaatattaa tggccttttg
     6421 tatttcctcg tatgcaatat ccggaaggga agttgtatca cctatagtca taatctttat
     6481 attgttttta tgtagttcgt tcaattcccg gcgcagatat tctacaagca aggacataat
     6541 agccttgatc tcgcccttgg gcctcttcca gttttcagtt gagaaggcat atagggtcag
     6601 atatttgact cctaattcag cactagtcgt aattatttcc ctcatcttct ctacgccctg
     6661 gcgatgccct gcgattctag gtagtccctt cttttccgcc caacgcccgt tcccatccat
     6721 aataattgaa atatgcctcg gtatgggctt gcttttaact gaatccatgg taaccctatt
     6781 atgctttatg agagcttttc tttgtttgat ttttttataa aaattcatat cctatcattt
     6841 ctccagagga caaccataat cgaaaaattt agatcccctt taaggcaaac cttcattaat
     6901 agtttgccct aaagaggtca gtcgggtgtc tcattgaaat taaaaactat taattctaca
     6961 gtcccgtcgg aaattatatt ctgtctgacg atccgcttaa tgcc
//
LOCUS       DASRNX010000126         8907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_449117,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000126 DASRNX010000000
VERSION     DASRNX010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449117"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..2071
                     /locus_tag="VFD89_05090"
     CDS             53..2071
                     /locus_tag="VFD89_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HZJ57599.1"
                     /translation="MGKKTDKKFTRILYGGDYNPNQWPREVWEEDMRIFKKASINSAS
                     INIFSWAKIQPSEETYYFDELDDIVEMLSKEEYDIVMATSTAALPAWMVKRYPEVART
                     DYYGRRHKFGQRHNACPNSLVYQKYAKALAEKLAERYGKNKNIICWHINNEYAGECYC
                     ENCEKAFRVWLKKKYKTIEALNRAWNMEFWGHTIHDWDEIVLPNALSEGIGENKTAFA
                     GISIDYRRFNSDSLLENFKMERDAVRRYDPDTPITNNLMGTYKGLDYFKWAKEMDIVS
                     WDNYPAYDTPWSMTAMRHDLMRGLKGQPFMLMEQTPSQQNWQPYNSLKRPGQMRAQSY
                     QTIAHGSDTIQFFQLRRTVGACEKFHGAVIAHVGHEDTRVFREVAQLGRELKGLDRIL
                     GSDNPSKVGIIFDWDNYWALEYTSGPHEDLMYVEQIHNCYRYFYDKKIPVDMIPTDGD
                     FGKYDIVMAPVLYMVKEGVGEALEEYVENGGILVTGFMSGIVDQSDNVHLGGYPGPLR
                     KMAGIWVEEIDALAPEQHNSVAFTDGSEYTCKMLCDLMHLEGAESLADYSSDFYADTP
                     AITRNEFGKGKVFYIGTDLEENALDKVLDQVTAEADVPPIIDEPTDLEVTCRESETEK
                     MYFIINFVKDEQPLPKCFVGEKDLLSGSELKDDTILKQYDVVMVSLPK"
     gene            complement(2173..3657)
                     /locus_tag="VFD89_05095"
     CDS             complement(2173..3657)
                     /locus_tag="VFD89_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HZJ57600.1"
                     /translation="MKNRLVVNADLGSTVINRNIYGHFSEHLGRCIYEGIWVGEDSPI
                     PNTHGIRKDVLDALKQMGIPVLRWPGGCFADEYHWKDGIGPRENRKKMINTHWGGVTE
                     NNHFSTHEFLKLCELLECEPYICGNLGSGTVQEMSEWVEYITFDGTSPMADWRRENGR
                     EEPWSLKYFGVGNENWGCGGNMRAEYYADEYRRYQTYVRSYGKNRIYKIACGPNTDDY
                     HWTEVMMREAGNMMDGLSLHYYTVPGTWEAKGSSTQFDEQDWFITMAKTLHMEELVTR
                     HSTIMDKYDPEKRIGLIVDEWGTWYDVEPGTNPGFLYQQNTLRDALVAGINLNIFNNH
                     ADRVQMANIAQTINVLQSVILTEGDKMLTTPTYHVFKMYKVHQDATLLPIDLKSAAYE
                     GRDSSIPQVSASASKNKDGIIHVSLCNLHHSEPTDLNLKLRGLLLGRITGRILTSDNM
                     NDHNTFQEPEKVQPKTFNDYNILDDNSLSINLPPKSVVLLAIEK"
     gene            complement(4054..5181)
                     /locus_tag="VFD89_05100"
     CDS             complement(4054..5181)
                     /locus_tag="VFD89_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZJ57601.1"
                     /translation="MKRRNKKIILALMVCFFSLMNLTGCLDFLLDGDWDTNPDEHYDA
                     KNPMEDAGEPDDPSNEDDLTLEPAGEDTVETTDDGIKNTKNPDAGDAEADLHNENNSD
                     DKKASESPKDRPSGSEKTTPKPGTKVNMDMWVMGSVNFRKNSNTHSDIISVLSVGTKV
                     KALEKTPDGWYKVDFEGTPGYVSGKYLSSKEVVVTKKDPPKEKPKSESKPGSKPDTAD
                     TGESPKPDSKKQSNSKYLADVEKGILKLCNKERQALGLTTLKWSDTARKIGRKKSKEM
                     YDKDYFAHESPYSGSVANQFELFGGLVLGKNASMIGENIAFAEGYEESEVTAKFWVNI
                     WMNSEGHKANILQPDYEYMGVGVYLGEDGRVYASQEFHSDL"
     gene            5441..7183
                     /locus_tag="VFD89_05105"
     CDS             5441..7183
                     /locus_tag="VFD89_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57602.1"
                     /translation="MASLALSTTAIQSFILKLPDYIENVYAMQEFDMDLAENVVLKGK
                     NYLDNPPDADKMADALEEMLDIYRGKRMAPGDFYDKLKGETGDLYLGWPYDKYIETKE
                     GQPAASIGLKSVLGAPGRKEKYNKSLLLKDIYLFQKEIENIFLKAAEKCKGGTEIIQK
                     DQIDLIYDCGLLYVQLRRAADSLFEINTDGKSEVDEIYKGLLSVNKRMLYFDDDEHLP
                     DLCITYDDGRSLAGYPDTVWWDGRYDEYHNHFHGSTQKKLSKDIFEPRYTPYIHLHDN
                     GSYQFNVNLYEGMALLKGKWEIDKEDSNIAYLHKPTNVVTDINFDRLELRRVSESKFV
                     LDGVENIGFPGFYSTSSGGDIYMKEGDESLITHKIEKYRSHDWAKLYMDFIDGTEMII
                     SEWNNFLTLDYDISGPSSVVLYDIDSDGVPELFIEEESEGLFYILTIRNNKVVYLGYA
                     NSGWVVKQRNIETGEDETFIYGTADTDGGYFDVGSLKLVGDELIFNVIFWETSWIEGE
                     ENKHEFRVGDKEADIDTYAKELQGFINSYERIGEARKYHSVNYYSDWGDFHLSREKFI
                     DVLCDYMESPGSLR"
     gene            complement(7240..8031)
                     /locus_tag="VFD89_05110"
     CDS             complement(7240..8031)
                     /locus_tag="VFD89_05110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZJ57603.1"
                     /translation="MTNAEILSIAMKQHAIDANCSPGDFSRSENVAVISRPHKDARRY
                     LNLPFFCDLITYGSNIVASVDERIFDFVREYMDTMHPHDCFELPQIHHLTQEFEKYGF
                     LPCYQAEYWLPDVDVLKVLPCKYETRVLEQCDFTKLYLPEWDNALSRSRPYLDMLGVG
                     AYDGDILIGLSGCSADCDTMWQIGIDVLPEYRRQGIAAALTSRLAVEILKRGKVPFYC
                     CSWSNLGSVRNAIKSGFRPAWVEHTAIEKEKALEWSANKHFPKSI"
     gene            complement(8727..>8907)
                     /locus_tag="VFD89_05115"
     CDS             complement(8727..>8907)
                     /locus_tag="VFD89_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57604.1"
                     /translation="AQPPIDEAAYELIYTTTGGFPRKINSLLTMALLTGLKEQMPILD
                     PDLFLKANAEVGIAL"
BASE COUNT         2504 a         1865 c         2056 g         2482 t
ORIGIN      
        1 atattgatgg aatctatcta tatacaaaca attaatttaa aggaggccta ttatgggtaa
       61 aaaaacggat aagaaattta caagaattct gtatggcggg gactataatc ccaaccaatg
      121 gcccagggaa gtctgggaag aggatatgcg catcttcaaa aaggcaagca taaacagtgc
      181 gagtatcaac atcttttctt gggcaaagat tcaaccctca gaggaaacct attattttga
      241 tgagctggac gacatagtcg aaatgctcag taaagaagaa tacgatatcg ttatggccac
      301 atcaacggcg gcgttgcctg cctggatggt aaagcgctat ccggaggtgg cccgcacgga
      361 ctattatggt cgcaggcata agtttgggca gcgccataac gcctgtccca atagcctggt
      421 gtaccaaaaa tacgccaagg ctctggcaga aaagcttgca gaacgttacg gtaaaaataa
      481 gaacatcatc tgctggcata taaacaatga atacgctggg gaatgctatt gtgaaaattg
      541 cgagaaggcg tttcgtgttt ggctcaagaa aaaatacaag accattgagg cgctaaatag
      601 ggcatggaac atggaattct gggggcacac catccatgat tgggatgaga tagtcctacc
      661 caacgccttg agtgagggga taggggagaa taagacggct tttgccggaa tctccatcga
      721 ttaccgccgc tttaattccg acagcttgtt agaaaatttt aagatggagc gagatgccgt
      781 tcgcagatat gatcccgata ccccaatcac caacaatctg atgggtacct ataagggtct
      841 ggattatttt aagtgggcaa aggaaatgga tatagtatca tgggataact atcccgccta
      901 cgatacacca tggagcatga ccgctatgcg ccatgacctg atgaggggtc ttaaggggca
      961 acccttcatg cttatggagc aaacacccag ccagcaaaac tggcagccct ataactccct
     1021 taaaaggccc ggacagatga gggcccagag ctaccagacc atagcccatg ggtcggatac
     1081 catccaattt ttccagctaa ggcgcaccgt cggtgcttgt gagaagtttc atggggcggt
     1141 catagcccat gtaggccatg aagatacccg ggtctttaga gaagtagctc agctgggcag
     1201 ggaattaaaa gggctggatc gcattttagg ttccgataat ccatctaaag tggggataat
     1261 atttgactgg gataactatt gggcactgga atacaccagc ggaccccatg aggaccttat
     1321 gtatgtggag cagatccata attgttatcg ctacttctac gacaagaaaa tccccgtaga
     1381 catgattcct acagatgggg actttggtaa atatgatata gtcatggcac ctgttctcta
     1441 tatggttaag gaaggggtcg gtgaagctct ggaagaatat gtagaaaatg ggggaatcct
     1501 ggtcaccggc tttatgagcg gcatcgtgga tcaatccgac aatgtccacc ttgggggtta
     1561 tccgggtccc cttcgcaaga tggccggcat ttgggttgaa gagatagatg ccttggctcc
     1621 ggagcagcat aattcggtag cctttaccga tggcagcgaa tatacctgca aaatgctatg
     1681 tgatttgatg caccttgaag gcgccgagag cttggccgat tattccagcg atttctatgc
     1741 cgatactccc gccataacca gaaatgagtt tggtaaggga aaggtattct atataggtac
     1801 ggatctggag gaaaacgctc tggataaggt gttggatcag gtaacggctg aggccgatgt
     1861 tcctcctatt atagatgagc ccaccgatct ggaagttacc tgcagggaat cagaaacaga
     1921 gaagatgtac tttatcataa acttcgtcaa ggatgagcag cccctgccaa agtgttttgt
     1981 aggagagaag gatttattat ccggcagtga actaaaagat gataccatat tgaagcaata
     2041 cgatgtagtc atggtatccc tacctaagta aaaacaagcc ccttcgtgga aacgaggggg
     2101 cctgtttttt tatgcaccac gcatgataca taatttgatg gtgaatattc gctattgggg
     2161 ttagagtaac gattattttt ctattgccaa taacacaacc gattttggag gcagattaat
     2221 tgaaaggcta ttgtcatcca gaatattata atcgttgaag gttttgggct gaactttttc
     2281 aggctcctgg aaggtgttgt gatcgttcat attgtcagag gtgaggatcc ggcctgttat
     2341 tctacctaag agcaatcccc taagcttaag atttagatcc gtaggctcgc tatggtgaag
     2401 attgcatagg gatacatgga ttattccgtc cttattttta gaagcggagg cactgacttg
     2461 gggtatagaa ctatctctac cctcgtatgc tgcgctttta aggtctatgg gaagcaaggt
     2521 ggcatcctgg tggaccttat acattttaaa cacatgatag gtgggggtcg taagcatttt
     2581 atccccctca gtcagtataa cggattggag cacgtttata gtttgggcta tgttggccat
     2641 ctgtaccctg tcggcatggt tgttaaagat attgaggttg atacccgcca ccagagcatc
     2701 ccgcaatgtg ttttgttgat agagaaagcc cggattagtg cccggctcta catcatacca
     2761 agtcccccat tcatcgacta ttagaccgat tcgtttttca ggatcatatt tatccatgat
     2821 ggtggagtgt ctggtaacca gctcctccat atgtaaggtc ttggccatgg taatgaacca
     2881 gtcctgttca tcgaattgag tagaggaccc tttggcttcc caggttcccg ggacggtata
     2941 gtagtgcagg ctcaaaccgt ccatcatgtt gccggcttcg cgcatcatta cctctgtcca
     3001 gtggtaatcg tcggtgttag ggccgcaggc aatcttatat atacggtttt tgccgtagct
     3061 gcggacatag gtttggtagc gacgatattc gtcggcatag tattcggccc tcatgttgcc
     3121 gccgcagccc cagttttcgt tcccgacccc aaaatattta agggaccaag gctcttcccg
     3181 gccattctcc ctgcgccaat cggccatagg agaagtaccg tcaaaggtaa tatattctac
     3241 ccattccgac atctcctgaa cagtaccgct ccccagattc ccgcagatat agggctcgca
     3301 ttctaagagt tcacacagct tcaaaaattc atgggtgctg aaatggttgt tctctgtaac
     3361 cccgccccag tgggtattga tcatcttttt gcggttttcc cttgggccta tgccatcctt
     3421 ccaatgatac tcatcggcga aacagcctcc cggccatcta aggacgggga tgcccatttg
     3481 tttcagggcg tctaggacat ccttgcgaat cccgtgggtg ttagggatgg gcgaatcctc
     3541 ccctacccag atgccctcgt agatgcacct accaagatgc tccgagaaat ggccatagat
     3601 atttcggttg atcacggtgc ttccaaggtc ggcgtttacc actagacgat tcttcatttt
     3661 gtatcctcct ccttgttaat ggttcatgat tatatggata gactacttct aactaaaaat
     3721 tcatcatctg cagataaatt taacctgttg tgctagttaa acatattggc aatgaagtca
     3781 tccgcttttc aatagcccat tacctttatc acacaatggg ttgatgaact atatgtttta
     3841 cgacaaacat tctacattag tatacgggtg tcgtaaaaca ttatcctcaa ttcatatgtt
     3901 acgaaaaata cattgcttta acgcaaaatc ggataactta actgattttg tattgaaaga
     3961 tctttttcgt aacatatata gcacagtgcg ataaaattag tttccgcatt gtttatctac
     4021 agatagttga cggataaagg gataatgacc tacttataga tcgctatgaa attcctggga
     4081 ggcatatacc ctgccgtcct caccgaggta aacacctaca cccatatatt catagtccgg
     4141 ctgtagtatg ttcgccttat ggccttcgct gttcatccat atgttaaccc aaaacttggc
     4201 tgtcacttca ctttcttcgt atccttctgc gaaggctatg ttttccccta tcatgctggc
     4261 gtttttcccc agaactaacc cgccaaacaa ttcaaattga ttcgctacag agccgctata
     4321 gggggattca tgggcaaagt aatccttatc atacatttcc ttggattttt ttctgcctat
     4381 cttccgtgct gtatcgctcc atttcagcgt ggttaaaccc aaagcctgtc tttccttatt
     4441 acaaagcttt aagatcccct tctctacgtc ggctaggtat ttagaatttg attgtttttt
     4501 ggaatcgggt tttggggatt ccccggtatc ggcagtatcg ggctttgatc ccggttttga
     4561 ttccgatttt ggcttttcct ttggcggatc ctttttagta actacaacct ctttgcttga
     4621 taagtacttg cccgacacat agcccggagt accctcaaaa tccaccttat accaaccatc
     4681 aggggtcttt tcaagggcct ttacctttgt tcccacggac aaaacgctga ttatatcgct
     4741 atgggtattg gaattttttc taaagttaac acttcccatc acccacatat ccatattaac
     4801 tttggttccc ggttttgggg tagttttttc ggacccactg ggcctatcct tgggggattc
     4861 gcttgccttt ttgtcatcgg aattgttctc attatgaaga tccgcttcgg catccccggc
     4921 gtcagggttt ttggtatttt taatgccatc gtcggtcgtc tctaccgtat cttctccggc
     4981 gggttccaag gtaagatcat cttcatttga tgggtcgtca ggttcacctg catcctccat
     5041 aggatttttt gcatcatagt gttcatcggg attagtatcc cagtccccat ccaataggaa
     5101 atccaggcat ccggtcaaat tcattagact gaaaaaacaa accataagag caagaatgat
     5161 ttttttgttt cgtcgcttca tataattccc cctcacaatc atacctatgt aatgtcgtat
     5221 cacagtatat tatagcagaa tattaagccg gatgggggca ataaggtcta gtttaatttt
     5281 atcttggagt tgtttatctg gtagtagtca tcgggcatat atccctcgac ccgtaaaaac
     5341 ctgtgtgatg taattgcttc caatgttata acaacttaat ccataactct ggcccattat
     5401 catgctgtgc tataaagata atgagtcact gatagggggg gtggcttcgc tggcgctttc
     5461 gaccaccgcc atccaatctt ttattctaaa gcttcccgat tatatagaaa acgtctatgc
     5521 aatgcaggaa tttgatatgg atctggcaga aaatgtggtg ttaaagggca aaaattattt
     5581 ggataatccc cccgatgcgg acaaaatggc cgatgcattg gaagaaatgt tggatattta
     5641 tagggggaaa aggatggccc ctggggactt ctatgacaaa ctaaaaggag agaccggtga
     5701 tctgtatttg ggttggccct atgataagta catagaaact aaagaaggac aaccggctgc
     5761 atcaatagga ttaaaatctg ttttaggagc cccggggcga aaagaaaagt ataataaatc
     5821 cttattgctt aaggatatct atctgtttca aaaggagata gaaaacattt ttttaaaggc
     5881 agcagaaaaa tgcaaagggg gaacagaaat tattcaaaag gatcaaattg atcttatcta
     5941 cgattgtggg ctcttatatg tgcaacttcg gagggcggcg gatagcttat tcgagataaa
     6001 taccgacggg aaaagcgaag ttgatgagat ctacaaaggg cttctgtccg tgaataagag
     6061 gatgctctat ttcgatgacg atgaacatct gccggatttg tgcatcacat atgatgatgg
     6121 tcgcagctta gcgggatatc cggataccgt ttggtgggat ggaaggtatg atgaatatca
     6181 taatcatttt catggcagta cacaaaagaa gctttcaaag gatatatttg aacctcggta
     6241 tacaccatat attcatcttc atgataacgg tagttaccaa tttaatgtta acctctatga
     6301 agggatggca ctcttaaagg gtaagtggga aatagacaag gaagacagca atatcgcata
     6361 ccttcataaa ccgaccaacg ttgtcacaga tatcaacttt gatagattgg aactgagacg
     6421 ggtttcagaa tccaaatttg tattggacgg ggtcgagaat atagggttcc ccgggtttta
     6481 tagcacctca tcgggcgggg atatctatat gaaggaggga gacgaatctt tgatcaccca
     6541 caagatagaa aagtacagat cccatgactg ggcaaagctg tatatggact ttatcgatgg
     6601 aactgagatg ataataagcg agtggaacaa ctttttaacc cttgattatg atatctcggg
     6661 tcccagttct gtggtgctgt atgacataga ttccgatggg gttccggaac tattcatcga
     6721 agaggaatca gagggcctat tctatatatt gaccataagg aataacaagg tggtctattt
     6781 gggttacgcc aattcagggt gggttgtaaa gcaaaggaat atagagactg gggaggatga
     6841 gacctttatc tatggcactg cagataccga tggaggatac ttcgatgtag gcagtctaaa
     6901 actcgttggg gatgagctta tatttaacgt tatattttgg gaaaccagtt ggatagaagg
     6961 cgaagaaaac aaacacgaat ttcgtgtagg ggataaggag gcggatattg atacatacgc
     7021 aaaggaatta cagggtttta tcaatagtta cgagaggata ggtgaagcga ggaaatatca
     7081 cagtgtaaat tattattccg attggggaga tttccatcta agcagggaaa agtttataga
     7141 tgtcttatgt gattacatgg agtctccggg atccttgagg taaatataga aaggggtttt
     7201 atccctttag aatacatctt atcaccatta ctgtacctcc taaattgatt ttgggaaatg
     7261 cttgtttgcg ctccattcca atgccttttc tttttctatt gctgtatgct caacccatgc
     7321 cggacgaaat ccgcttttga tggcgttacg tactgaaccg aggttagacc atgagcagca
     7381 atagaacgga accttacctc tcttaagaat ttccacggcc aaccgagaag ttagggcagc
     7441 agcaattcct tgtcggcgat actccggtaa aacgtcaatg ccgatctgcc acatggtatc
     7501 gcaatcggca gaacatccgg aaagacctat gagaatatcc ccatcatatg caccgacacc
     7561 gagcatatcc agatacggac gagatcgtga aagggcatta tcccattccg gaagatataa
     7621 tttggtaaaa tcacactgct ctaatactct ggtttcatat ttgcatggaa gcacttttag
     7681 cacatcgaca tccggtagcc aatactcagc ctgataacag ggcaaaaaac cgtatttttc
     7741 aaattcctgt gtcagatgat gaatctgcgg tagttcaaag caatcgtggg ggtgcattgt
     7801 gtccatatac tcccgcacaa aatcaaagat gcgctcatcc acagaggcga caatattaga
     7861 accgtatgtg atcaagtcgc aaaagaaagg caggttcaaa taccgacggg cgtctttatg
     7921 gggccttgaa atcacggcta cattctccga tcgagaaaaa tcccccgggg agcagttcgc
     7981 atcaatcgcg tgttgcttca ttgctatgct caagatttcg gcatttgtca tttttatagt
     8041 tcccttctta tctgccccat cagccatgta ttaaaggaga tcgtctacgg tatattctac
     8101 cacggtaaaa atcgggtttc aataaaatgt tgcaaaaaag ttaaggtatc gtaattgctt
     8161 attttcaaat atcgcttggc tttgcttggc tggtctgttt agcggaaacc cttatttgat
     8221 acggatgggg ttcgcagggg caaatcagtt agtgtataat attagttggc aaaaaggtat
     8281 aaaaaggaag aagtcataaa gaccccttcc ttatacatga atcatccatt ataatagaat
     8341 tgacaaaaaa cattgaatgg ggtgcgccag ttcggcgtta aatatattgt tacacggcac
     8401 aataaagtag cttaagaaaa gcataataaa atagcataaa aagtggatgc aaaaggtgca
     8461 aagactgcag tttaaataaa ccgccgcatg gaaaaaccat acaaactgca gattaagcta
     8521 cccttaacag ggtagttggg gctttgcccc aagccccagg atttatcgct ttggttctcc
     8581 aaaagacgca aaagaaaatc gcatagaaaa tgggcaactc aatgtgccca aattcttttg
     8641 catcttttta tagaatcccg gaaggcgctc gggcctaagc ccagcgttgc cctatcctcc
     8701 ataccgggca agagccaatg gtaatatcaa agtgcaatgc ctacctctgc attagccttg
     8761 aggaataaat caggatctaa gataggcatc tgttccttaa ggcctgttag aagagccata
     8821 gtaagcaggg aattgatttt ccttgggaat ccgcctgtcg tagtatagat aagttcataa
     8881 gcggcttcat caatgggcgg ctgagct
//
LOCUS       DASRNX010000127        11258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_449158,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000127 DASRNX010000000
VERSION     DASRNX010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11258
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449158"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..292)
                     /locus_tag="VFD89_05120"
     CDS             complement(62..292)
                     /locus_tag="VFD89_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57605.1"
                     /translation="MKIPDVVGQELDKGLEILKNGNGLFSIIINECTLPDDFYKTAKA
                     DGIKRIVRQNIISGGALELIIFNFNETPDRPL"
     gene            complement(293..814)
                     /gene="frr"
                     /locus_tag="VFD89_05125"
     CDS             complement(293..814)
                     /gene="frr"
                     /locus_tag="VFD89_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZJ57606.1"
                     /translation="MDKTILNLNRELGSLRAGRANPQLLDRIVVDYYGVATPLNQVGN
                     ISVPEPRLLVIAPWESKMISIIEKEIMKSDLGITPNNDGKVIRLVFPELTEERRKELV
                     KIAYKMGEEAKVAIRMVRRNSNDEIKRMKRDSEYSEDDLKIAENRVQEITDEFVEKID
                     GIIEDKEKEIMEV"
     gene            complement(853..1566)
                     /gene="pyrH"
                     /locus_tag="VFD89_05130"
     CDS             complement(853..1566)
                     /gene="pyrH"
                     /locus_tag="VFD89_05130"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZJ57607.1"
                     /translation="MLDPIYDRLVLKISGEALAGDGGYGIDPKILDLISEQVIEIMDM
                     GVEVAIVVGGGNIWRGRSGVGMDRTTADHMGMLATVINALALQDALEKRDVPVRVQTA
                     IEMRQIAEPYIRRRAVRHLEKGRIVIFACGTGNPYFSTDTTAALRAAEIEAEVILLAK
                     TIDGVYDDDPKINPKASKFDKISYMEVINRGLGVMDTTAITLCMENKIPIVVFGLENR
                     ENIKAAILGKKIGTTIEEV"
     gene            complement(1853..2500)
                     /gene="tsf"
                     /locus_tag="VFD89_05135"
     CDS             complement(1853..2500)
                     /gene="tsf"
                     /locus_tag="VFD89_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZJ57608.1"
                     /translation="MITASMVKELRERTGVGMMDCKRALQDSNGDVDKAVELLRERGL
                     ASAAKKAGRIAAEGIVESYIHGGGRIGVLVEVNCETDFVAKTDEFKTFVRDIAMHIAA
                     TNPQYVTREQIPQEDLDKEKDILKAQALNEGKPEKIVDRMIVGRIEKYIKEICLMEQP
                     FVKDPDKTVQDVLNEQIATIGENMSVRRFVRFEKGEGLEKRQDDFLDEVMSQARG"
     gene            complement(2855..3568)
                     /gene="rpsB"
                     /locus_tag="VFD89_05140"
     CDS             complement(2855..3568)
                     /gene="rpsB"
                     /locus_tag="VFD89_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZJ57609.1"
                     /translation="MAVVSMKELLEAGVHFGHQTRRWNPKMATYIFTERNGIYIVDLQ
                     KTVRKMEEAYNFVRDLAVEGKEILFVGTKKQAQEAIEYEAQRCGMYYVNQRWLGGMLT
                     NFKTIRKRIDELHRLNEMEKDGSFEILPKKEVIKLRLERDKLERNLGGIKDMKRLPAA
                     IYIVDPRKERIAIAEARNLGIPIIAIVDTNCDPDEIDYVIPGNDDAIRAVKLITSKMA
                     DAVLEGRQGEQFTDANEEQ"
     gene            complement(3723..3929)
                     /locus_tag="VFD89_05145"
     CDS             complement(3723..3929)
                     /locus_tag="VFD89_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57610.1"
                     /translation="MKNFSLSAFCIGLGFGMIFSYFFIISNITRSDPIAEIENIPNEI
                     VIQRAKEIGLIDPYEGVYKNSMEE"
     gene            complement(3926..4405)
                     /locus_tag="VFD89_05150"
     CDS             complement(3926..4405)
                     /locus_tag="VFD89_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57611.1"
                     /translation="MDKNFYMLLWGGLFFLFIGLWMVKRDREWMQIWNANMDEKENSL
                     YELYESLEEIMNEMYYLYREGYNIEAGPPPAMDDGYDPVLADQDPQDHRDDTVHETQA
                     ETKKDTQDQNPEGHRYRLITDLSDQGLSVEEIARRLGIGKGEITLVLKFHKGRERIQ"
     gene            complement(4414..4734)
                     /locus_tag="VFD89_05155"
     CDS             complement(4414..4734)
                     /locus_tag="VFD89_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57612.1"
                     /translation="MSIRPVDMQVAIQRSDQYSKEFNKNIEVGNYQHETAIETQKQVF
                     QSQREVNPSEGLKHNAITRDDDSKTNTGKKRYKNDTESRSHNKKSDAVPTDDDKGVFI
                     DIII"
     gene            complement(4745..6352)
                     /locus_tag="VFD89_05160"
     CDS             complement(4745..6352)
                     /locus_tag="VFD89_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HZJ57613.1"
                     /translation="MRSVSGDTYFRLEYEDDGIYITVSPSKEGSSKEDELNLLSYIKR
                     KEIVGVNSTAVYNAIYANPDERIRIADSQDEKLLDQEMGIRVIKGGMEARAKLLPGDN
                     DKRLTPMKAIELLHRYGIVYGIDESAIEKMLDQGIYYVEETVAHGKPPQNGRDAMIKY
                     HVDFNHKAKPHILENGTVDYRHLGLIHNVVKGQPLAELIPATEGIPGKTVAGKTITAR
                     SGKKLPLPMGKNTVLSEDKLSLTASIDGKAEMIDGKIHIFAIYEIPGNVDNSTGNIDF
                     VGNVIVNGNVLTGFEIKAGGYIEVRGVVEGASISAKGDVLLRQGMQGMGKGRVQSHGN
                     VIARFIENSTVEAKGDILTEVILHSTISCGGRVQVKGRRGLIAGGSISAGIDISAINI
                     GSPMATITELEVGVSPLLREEHSTLVDEMEKTMQELKKADQILTLLNKVESKENLSPD
                     KAKMKLKAIRTKLASTQKVPKMRARIMELEDIFKGASTGKINIGGTVFPGVKVVIGTS
                     VQYIKEQEEHVTFIREHGDVIRTSFLG"
     gene            complement(6363..7142)
                     /locus_tag="VFD89_05165"
     CDS             complement(6363..7142)
                     /locus_tag="VFD89_05165"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HZJ57614.1"
                     /translation="MILKNDGAIESLWIKFKNDGDIQLRNQLIVHYSDLVNRVVNRIA
                     IKYVNYMDIDDLVGYGIFGLIDAIEKFDLSKGIKFETYASFRIRGEIIDRIRQQDWTP
                     RSLRDKARQAERALEELEMKLGRNVSDEELADHMGIKLGELNTLMEQIHTSVIISLDS
                     HILDSIDEPHGLTGNADAPEEHAIAMEMKGILGQAIEQLTEKEQMIVDLYYFQELTLR
                     EIGMVIGVSESRVSQLHSKILLKLKGKLSRTGLYLKEGTHG"
     gene            complement(7190..7678)
                     /locus_tag="VFD89_05170"
     CDS             complement(7190..7678)
                     /locus_tag="VFD89_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925472.1"
                     /note="catalyzes the conversion of glutamine residues to
                     glutamate on methyl-accepting chemotaxis receptors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HZJ57615.1"
                     /translation="MIQRVKVGMADLNTVISPGVLVSLGLGSCVGIALYDGHVKVAGL
                     AHIMLPSSTSIKNNENRAKFADTAILQLIDDMIVLGAKQNRLKAKLAGGAQMFAFNSR
                     DNSPLKIGERNIVSTLDVLMELKIPVIGQDTGGNYGRTVELYAETGDYLIKTIGHGVK
                     RI"
     gene            complement(7675..8286)
                     /locus_tag="VFD89_05175"
     CDS             complement(7675..8286)
                     /locus_tag="VFD89_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HZJ57616.1"
                     /translation="MTFLNDIYLDAFKEVGNIGAGNAATSLSKLLNRRINMNVPEIKI
                     LRFNEVETVVGGAEKLVAGIYLEFVGDIRGTILYILDKDSANNLLHLLMPNYCRDHQG
                     DFSKIESSALQEVGNILASAYLGSLSALTGLYVKPSTPALAYDMAGAILSVPLIEFGQ
                     VGEQAFFIETLFIDGLDRIKGNFFLIPEIDSYFIILKALGVFR"
     gene            complement(8283..8747)
                     /locus_tag="VFD89_05180"
     CDS             complement(8283..8747)
                     /locus_tag="VFD89_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZJ57617.1"
                     /translation="MADNSRVKGNSAGRQFISFSLGDGIYGLDILMTESIERVSDITR
                     VPKAPKYVPGIINVRGDIIPLVNLRIRLGIGEKQYSEESRIIITTFEGYRVGVTVDKV
                     NDVFSAPDTDIQKREAVFNDGINEHVSEIVNLEGEHVLILDLTKVLDIGGLV"
     gene            complement(8761..10749)
                     /locus_tag="VFD89_05185"
     CDS             complement(8761..10749)
                     /locus_tag="VFD89_05185"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HZJ57618.1"
                     /translation="MDSDKYIGLFVEEAKEHIQILNDNLLRLEAEANNENVEPLNEVF
                     RAAHTLKGMAGTLGFGKITDIAHAMENILDEIRNQNMGINASIIDILLECTDAMEELI
                     LDVSRSGDEGPQEISELLLKLNKPMHSFDNSTGNGSDYGAVELDEYQSSVINTGISQG
                     LIPYWIEVVLQPTCLLKAARAYVVFQALEEFGEIIKTLPQVQDIEEERFEDTFGMLLL
                     SREEDSIIKQRLDLVSELHSVKIHRISHNLDLDANDKSVGPDDKAKEPVSGNQRILRD
                     KIYTQTRSIRVDTEKLDNLMNLVSELITIKNSLMDVTGSDVESSTREYMEYLARITNG
                     LHDTVTKVRMVPIEMVFSRFPRVIRDLSRNIKKEIALNIIGSETEIDRTIIDEIGDPL
                     IHLLRNAVDHGIETPEERVEAGKDPVGRVDLMAYHDGNNVVIHVEDDGHGIDLENIAN
                     SAMEKGLISDIDLGSLEEREIIGLMFHPGFSTAGTISNISGRGVGMDVVKTKVESMGG
                     TIDVTTQIGVGSRFTIRLPLTLSIIQALLVEVGNEIYAIPLNSIQEIVDISGSDIKRF
                     EGHETIPYNDILLPLIRLESELDTELMNSRQDQIMTMIIVKKGDRRFALSIGNLIGQQ
                     EIVIKPLGKYLSNNKIISGATILGNGKVALILDINHLV"
     gene            complement(10773..>11258)
                     /locus_tag="VFD89_05190"
     CDS             complement(10773..>11258)
                     /locus_tag="VFD89_05190"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZJ57619.1"
                     /translation="EIYFFKEEASFLFHGRVKKRFRINNAVVVSITRTSPIQRIQRRE
                     FYRLRVILPVDFWLLDDDIEDKPTVYKGYSIDISGGGLKLNTDYCLEVNTAIECRIDL
                     GNERQLQIKGKVIRVNPAGGSGYRYELGISFCCVSHRVREDLIRFIFSKQRELREKGL
                     I"
BASE COUNT         2869 a         2843 c         1890 g         3656 t
ORIGIN      
        1 ggacaacctt aatcgaaaaa tttagacccc ctttaaggca aaccttcatc attagtttgc
       61 cttaaagagg tctatcaggt gtctcattga aattaaaaat tattaattct agggctccgc
      121 cggaaattat attctgtctg acgatccgct taatgccatc agcttttgcc gtcttataaa
      181 agtcgtcagg cagagtgcat tcattgatga ttatgctaaa taatccgtta ccattcttta
      241 gtatttccag ccctttatcc aattcttggc ctaccacatc gggaattttc acctatacct
      301 ccataatctc tttttcctta tcctcaataa tcccatctat cttctctaca aattcgtcag
      361 tgatttcctg gaccctgttc tcggcgattt tcagatcatc ttcggagtat tcggaatccc
      421 ttttcatcct tttaatctca tcattggaat tgcggcgaac cattcgaata gctacttttg
      481 cttcctcgcc catcttatag gcaatcttaa ccagttcctt ccttcgttcc tcagttaact
      541 ccggaaatac caaacgtatg accttaccgt cgttattggg ggttattccc aaatctgatt
      601 tcataatttc cttttcaata atcgaaatca tcttactctc ccatggggct ataaccaata
      661 gcctgggctc gggaacggaa atgttaccta cctggtttag cggagtagca actccgtaat
      721 aatctaccac aatcctgtca agtaattggg gattagctct tcccgcccga agactcccta
      781 attctctgtt tagatttaat atagttttgt ccatggtttc ctcggtctcg tcataaagta
      841 tctcaagcac tgctatacct cctcaatcgt tgttcctatt tttttaccca ggatggcagc
      901 tttgatgttc tcccgatttt ctaaaccgaa gacgactatg ggaattttat tttccataca
      961 tagtgttatg gcggtggtat ccattacccc taagcccctg ttgatgactt ccatatagct
     1021 gatcttgtcg aatttactag cctttggatt gatcttcggg tcgtcgtcat aaaccccgtc
     1081 gatggtcttg gctaacaata taacctcagc ctcaatttcg gccgcacgta gggcggctgt
     1141 agtatctgta gaaaaataag gattaccggt accgcaagca aatataacta ttctaccctt
     1201 ttccaggtga cggactgcgc gtctgcgaat atagggctcc gcgatctgcc tcatttcgat
     1261 agcagtctgg acccgtacgg ggacatccct tttttcaagg gcgtcttgaa gtgccagggc
     1321 atttattacc gtcgccagca tacccatatg atcggcagtg gtgcgatcca tgcctacacc
     1381 cgaccttccc ctccaaatat ttcccccgcc gaccactata gccacttcaa cgcccatatc
     1441 cataatctca atcacctgtt cggatatcag gtcaaggatt tttgggtcta taccgtatcc
     1501 tccgtctccg gcaagcgctt ctccactaat tttgagaaca agcctgtcat aaatcggatc
     1561 taacatcatt aagaaacctc catcccatat agttatattt ctacgtattt tgcctatctc
     1621 ctcttttttg gggattaagg ccttcatctt agtttgattt tataatagaa aagtcgcaaa
     1681 ttttgttaca ttaacttgat tcaaccgact cacagattcc acgtcgccat ggacatcctt
     1741 gcgtttggct aatggttggc aactgccagc ccccatagcg gactctcacc gccaagttat
     1801 gcgccatgca cggcgcactc ataaaaaaag agaacacaga tgtgttcctt ttttagcccc
     1861 ttgcttggct cataacctca tcgagaaagt catcttgtct cttttctaaa ccttcgccct
     1921 tttcaaagcg gacaaatctt cgcacgctca tgttttcccc gatggtggct atttgctcat
     1981 tgagaacgtc ctgaacagtc ttatccggat ccttaacaaa aggttgttcc ataaggcata
     2041 tttctttgat atatttttca attcttccga cgatcattct gtcaacaatc ttttcgggct
     2101 ttccttcatt taatgcttgg gcttttagga tatccttttc cttatccaaa tcctcctggg
     2161 gaatctgttc acgtgtgacg tactgtggat tggtcgcagc gatatgcata gctatatccc
     2221 ttacaaaggt cttgaattca tcggttttgg ctacaaaatc cgtctcgcaa ttcacttcaa
     2281 ccaggacacc gatcctcccg ccgccatgta tgtaagactc tactatccct tcggctgcaa
     2341 ttctgccggc ttttttcgct gcggatgcaa gccctctctc ccgcaataat tcaacggctt
     2401 tatccacatc cccatttgaa tcctggaggg ctctcttaca atccatcatc ccaactccgg
     2461 tgcgttccct aagctctttt accattgaag ctgtaatcat gtaacattcc tcccttatct
     2521 aaaatgtatt actaaaataa ttatttttct gcagtataag aattgtattt atcaggtatt
     2581 gtaccacaag taaacaaaaa cgaatctatc atagccaaat catttgaatt aatgtgttat
     2641 gccgttcaat aatgtaaaat atgccatcaa aggccacctt gcatttaagg ccattttccg
     2701 taacatattg cataatatgc acaattaaat acaaataaat actaaaggct acagtagata
     2761 cgccctataa cattggaata atgatttctt atattccaaa ccttcatacc aacaattagg
     2821 ggaagaacgg ataccctttc ttccccatta tccattattg ctcttcatta gcgtctgtaa
     2881 actgttcgcc ttggcgtcct tccaagaccg catctgccat tttcgatgtt atcagcttaa
     2941 cggcgcgaat tgcatcatcg tttccgggga ttacataatc aatctcgtcc ggatcacaat
     3001 tggtatcaac gattgctata attgggatac ccaagtttcg ggcctccgct atggcaatcc
     3061 tttctttcct cggatcgacg atataaattg ccgcgggaag tctcttcata tcctttatgc
     3121 cgcctaggtt tctctccaat ttgtctctct ccagtcgaag cttaattact tctttcttgg
     3181 ggagaatttc aaacgacccg tctttttcca tttcgtttag tcgatgcaat tcatcgatcc
     3241 tcttacggat ggttttaaaa ttggtgagca tgcctcccag ccacctttga ttaacatagt
     3301 acataccgca tctttgtgcc tcgtattcga tggcctcctg ggcttgcttt ttggtcccca
     3361 caaacaatat ctccttaccc tctacggcta agtcccttac aaaattatag gcttcttcca
     3421 tctttcggac ggttttttgt aaatctacaa tgtagatacc gtttctctcg gtaaagatat
     3481 atgttgccat tttggggttc catcggcgtg tttgatgtcc aaaatgaaca ccggcttcca
     3541 gcaattcttt catgctaact actgccatat ccattcacct cctcagttat tcctcccctt
     3601 ctttcatccg aaaaagcaac ctctatgagg cactgatttc ccgtcaaaaa gggtgtgttt
     3661 tcaccgtcag tagtatagca taaccaaaac caattttcaa ggactatttt ccattatagt
     3721 tgtcattcct ccatagaatt cttatagaca ccctcatatg gatcaatgag tccaatctct
     3781 ttagcccttt gtatgacaat ttcgttgggt atattctcta tttcggctat gggatcggat
     3841 ctagttatat tgctgataat gaaaaaatag ctaaagatca taccaaaacc aaggccaata
     3901 caaaacgctg ataatgaaaa attcttcatt gtatgcgctc ccgtccttta tgaaatttta
     3961 acactagcgt tatctcccct tttccaatcc ctaatctcct cgctatctcc tcaacagaaa
     4021 ggccctgatc gcttaaatct gtgattaggc ggtatctatg gccttctgga ttctgatcct
     4081 gggtatcttt tttcgtttcg gcctgggttt catggaccgt atcatctcta tggtcttggg
     4141 gatcctggtc cgccaatacg ggatcatatc catcatccat agccgggggg ggaccggcct
     4201 ctatattata tccctctcta tagagatagt acatttcatt cattatttct tccagggatt
     4261 catagagctc ataaagacta ttttcctttt catccatatt ggcattccat atttgcatcc
     4321 attccctgtc cctttttacc atccataagc caatgaatag aaaaaacaaa ccaccccata
     4381 atagcatgta gaaattttta tccataagcc acctcatata ataatatcta taaaaacccc
     4441 tttatcatcg tccgtgggca cagcatcact ttttttatta tgacttcttg attctgtatc
     4501 atttttatat cgtttcttac cggtgtttgt tttgctatca tcatctctgg taatagcgtt
     4561 atgttttaag ccctcggaag ggtttacttc gcgctgggat tgaaaaacct gcttttgagt
     4621 ctctatagcg gtttcatgtt gataattgcc aacttcgata tttttattaa attctttcga
     4681 gtattgatcg gatctctgga tcgccacttg catatcaacg ggtcgaatcg acataaaatc
     4741 cctcctaacc tagaaaagaa gtccttatca catctccatg ctctctgata aatgtcacat
     4801 gttcttcctg ctcttttata tattgtactg atgtcccaat gacaactttt acgcccggga
     4861 aaaccgtacc cccaatattt attttccctg tggatgctcc tttgaagata tcctccagtt
     4921 ccataattct ggccctcatc tttggtacct tctgggtaga tgccagcttt gtcctaattg
     4981 cttttaattt catcttcgcc ttgtccgggg atagattctc cttggattcc accttgttca
     5041 atagggtaag gatttggtcc gcctttttta attcctgcat agtcttttcc atttcatcga
     5101 ctaacgtaga gtgttcttct cgcagcagcg ggctgacccc aacttctagt tcggtaatgg
     5161 ttgccatcgg ggagccaata tttatggcgg atatatcaat tcccgcagaa atagatcccc
     5221 ctgcaatcag accccttctc cctttaactt ggaccctgcc accgcagctg atggtgctat
     5281 gtagtatgac ttcggtaaga atatcccctt tagcctctac agtactgttt tcaataaaac
     5341 gggctattac gttaccatgg gattgaacac gacctttacc catcccctgc ataccctgtc
     5401 tgaggagcac atcccctttc gcggatatac ttgccccttc tacaacgccc cttacttcga
     5461 tatacccgcc ggcttttatt tcaaatcccg taagcacatt gccattgact ataacatttc
     5521 ctacaaagtc aatgttcccc gtggaattgt ccacatttcc ggggatttca tagatggcaa
     5581 agatatggat ttttccgtct atcatctcgg ctttcccatc tatggaagcg gtcaaagaca
     5641 gcttatcctc ggaaagaacc gtatttttac ccataggtaa cggtaacttt tttcctgatc
     5701 tggctgtaat ggttttaccc gcaacggttt tcccgggtat tccttccgta gcagggataa
     5761 gttcagccaa tggctgtccc tttacaacgt tgtgtattag acccaaatgt ctgtagtcaa
     5821 ccgttccatt ctctaagata tgcggtttag ccttgtgatt aaagtcgaca tgatatttaa
     5881 tcatcgcgtc ccggccgttt tggggcggct taccatgggc aacggtttct tccacgtaat
     5941 aaattccttg gtccagcatt ttttctattg ccgactcgtc tatgccataa acaataccat
     6001 atcgatgcag tagctctatt gccttcatgg gggttagcct tttatcgtta tcccctggca
     6061 aaagcttagc gcgggcttcc attcccccct tgatcaccct aatacccatt tcttggtcca
     6121 gtagcttctc atcttgtgaa tcagcaattc tgatcctttc gtccggattt gcataaattg
     6181 cgttatatac agccgtactg ttgacaccca ctatttcctt tctctttata tagcttaata
     6241 ggtttaactc gtcttccttt gaagaccctt ctttagacgg cgaaactgta atatatatgc
     6301 cgtcatcctc gtattcaagg cgaaaatagg tatcaccaga aacgcttctc atagatttac
     6361 tcctacccat gggttccctc cttaagatat aagccggttc tgctgagctt gccttttagt
     6421 ttcaacaata tcttcgaatg caattgtgac accctagatt cggatacacc aataaccata
     6481 ccgatttccc taagggttaa ttcctggaaa tagtacagat caacaatcat ttgttccttc
     6541 tctgtcaact gttctatggc ttgtcctaat atacccttca tttccatcgc tattgcatgc
     6601 tcttcgggtg cgtctgcgtt acctgtcaac ccatggggtt cgtcaatgct atcaagaata
     6661 tgactatcta aggaaatgat taccgaagta tggatttgct ccattaaggt gtttagttcg
     6721 cctagtttta ttcccatatg atcggccagt tcctcgtctg acacattccg gcccagtttc
     6781 atttccaatt cctccagggc tctctctgcc tgccttgcct tatccctcag actccttgga
     6841 gtccagtcct gctgacgtat acgatctatt atttcacccc gaatcctaaa agaagcgtag
     6901 gtttcaaact taattccctt actgagatca aatttttcaa tagcatcgat taatccaaag
     6961 atcccgtacc ctactaaatc gtctatatcc atataattca cgtatttaat cgcgattcta
     7021 tttacgaccc tatttactaa atcgctatag tgcacaatca attgatttct cagctgtata
     7081 tcgccatcat ttttaaattt gatccatagg ctttctatgg caccgtcatt cttaagtatc
     7141 atacgccccc cttaatccgt ataaacatca aggttgtgag tttaaatgac taaatccgtt
     7201 taactccatg gccaattgtt ttaattaaat aatcgcccgt ttctgcataa agttcgactg
     7261 ttctgccgta gttcccgccg gtatcctgcc cgattacagg tatctttaat tccattaaaa
     7321 catctagagt ggaaacgata tttctttcgc caattttcaa cgggctgttg tcacggctat
     7381 taaaagcaaa catctgcgct cctcccgcaa gcttggcctt taatctgttt tgttttgcac
     7441 ctaagacaat catatcatca atcaattgaa gaatagcggt atcggcaaat ttggccctat
     7501 tctcgttgtt ttttattgaa gtgctggatg ggagcatgat atgggccagt cctgctactt
     7561 taacatggcc gtcataaaga gcaattccca cacaggaccc cagacccaat gataccaaga
     7621 ctcccggcga tattacagta ttaagatcgg ccattcctac cttcaccctt tgtatcatct
     7681 aaaaactcct aatgctttaa gaataatgaa atatgagtca atctccggta tcaagaaaaa
     7741 atttcccttt atcctatcca atccgtctat aaacagggtc tcaataaaaa atgcctgctc
     7801 gccaacctgc ccaaattcaa tcaaaggtac gctgaggatt gctccggcca tatcgtacgc
     7861 taatgcaggt gttgaaggtt tgacatatag acctgtcagg gcagaaaggg atcctaaata
     7921 ggcactggct aagatatttc ccacttcctg aagcgctgat gattctattt tagagaaatc
     7981 accttggtga tccctacaat aattaggcat caataagtgt agaaggttgt tagctgagtc
     8041 cttatccaaa atatacaata tagtgccacg aatatctcca acgaattcaa ggtatatacc
     8101 ggcgactaat ttttctgccc caccaacaac agtctcaacc tcattaaagc gtaatatttt
     8161 tatttccggg acattcatgt ttatccttct gttcaacagc tttgatagtg acgttgctgc
     8221 gttgccagca ccgatattgc ctacttcctt aaaggcgtcc agatagatat cgtttaaaaa
     8281 ggtcatacca atccccctat atccaaaacc ttcgtcaaat ccaatatcaa tacatgctcg
     8341 ccttccagat tgacgatctc tgaaacatgc tcatttatgc cgtcattaaa aacagcttcc
     8401 cgcttttgta tatccgtgtc aggagccgaa aagacatcgt taaccttatc caccgttaca
     8461 cctacccgat acccttcaaa agttgtaata atgatacggc tttcttcact atattgcttt
     8521 tctcctattc ccagcctgat tctcaaattg actaatggaa taatgtctcc tcttacgtta
     8581 attattcccg gaacgtattt tggggctttg ggtacccgcg tgatatcaga aactctttct
     8641 atagattcgg tcatcagtat atccagcccg tatattccat cacctagact aaaactaata
     8701 aattgtctgc cagcagaatt gccttttacc ctgctattat cagccatttc cgccttcccc
     8761 ctatactaga tggttaatat ccagtattaa agccactttt ccgtttccga gaatggtggc
     8821 accggatatt attttgttat tggataaata tttacctaaa ggttttatca cgatttcctg
     8881 ttgtccaatc agattgccta tggataacgc gaaacgtcta tcgccttttt tgacgattat
     8941 cattgtcata atctggtcct gtcggctatt catgagctct gtatctaatt cactttcaag
     9001 gcggatcaaa ggaagcaaaa tatcattata tggtattgtc tcatggccct caaatctctt
     9061 tatatctgac cctgaaatat ctactatttc ctgaatagag tttaatggta tcgcataaat
     9121 ttcattcccg acttccacca gcaaagcctg gatgatggag agggtcaaag gaagacggat
     9181 cgtaaatctg ctccctaccc caatttgagt tgtcacatca attgttccac ccatggactc
     9241 cactttggtt ttgacgacat ccatccctac gcccctgccg gaaatattgg aaatggttcc
     9301 tgcggtgcta aacccgggat gaaacataag cccgattatt tccctttcct ctaaagatcc
     9361 caaatctata tccgatatta aacccttttc catggcgcta ttggcgatgt tttcaagatc
     9421 gattccgtgg ccatcatcct ctacgtgaat aaccacattg ttaccgtcat gatatgccat
     9481 caaatccacc ctaccaactg gatccttacc cgcctctacc ctctcttccg gcgtttctat
     9541 cccatgatcg acagcatttc ttaatagatg gattaatgga tcacctattt catcaattat
     9601 agtccgatct atctccgttt ctgaacctat gatgttcaat gctatttcct ttttgatatt
     9661 tctcgacaga tctctgatca ccctgggaaa ccgactaaat accatctcta tgggtaccat
     9721 tcttaccttt gtgacggtat catgaagtcc gttagtaatt ctggctaagt actccatata
     9781 ttccctggtg ctggattcta catcacttcc cgtcacatcc attaacgaat tctttattgt
     9841 aattagctcg cttaccagat tcattagatt gtccagtttt tctgtatcaa cccttatact
     9901 gcgggtttgt gtatagattt tatcccgtaa tatccgctgg ttaccggaca ctggctcctt
     9961 ggctttatca tctggaccaa ccgacttatc attcgcgtcc agatccaaat tgtgcgaaat
    10021 cctatgaatt ttaacggaat gaagctctga aaccaaatcc aacctttgct ttattattga
    10081 atcctcttcc ctggaaagaa ggagcatacc aaaggtatct tcaaatctct cctcctcaat
    10141 atcctgaacc tggggcaatg tcttgatgat ctccccgaat tcttccaggg cctggaaaac
    10201 aacatacgcc cttgcagcct tcagtaggca tgtcggttga agcactactt caatccaata
    10261 gggaatcaat ccctggctaa tccctgtgtt aataacactt gactgatatt catcgagttc
    10321 tacagcccca tagtcggatc cattgcctgt gctattatca aaggaatgca tcggtttatt
    10381 gagctttaac agcaattctg atatctcttg cggaccctcg tcgcccgacc ttgaaacatc
    10441 caggatcagc tcctccatag catctgtaca ttctaacaat atatcaataa tggatgcatt
    10501 tatccccata ttttgattcc ggatttcgtc taggatgttc tccatagcat gggctatgtc
    10561 cgtgattttt ccaaatccta gtgtgcccgc cattcctttt agggtatggg ccgcacggaa
    10621 tacttcattc aatggctcaa cattttcatt atttgcttct gcttccagtc tgagcaagtt
    10681 atcattgaga atctgtatat gctccttggc ctcttccaca aagagcccaa tgtatttatc
    10741 gctatccatg tttccatgac accccctaaa tattatataa ggcccttttc tctcaattca
    10801 cgctgtttag aaaaaataaa ccttattaaa tcctcccgaa ccctatgaga aacgcaacaa
    10861 aatgatatac caagctcata tctgtatccc gatccgccgg ctggattaac cctaatcact
    10921 ttccccttta tctgtaattg tctttcatta ccgaggtcaa tcctgcattc gatagcggta
    10981 ttcacttcca ggcaataatc cgtgtttaat ttcaaaccgc ccccgctaat atcaattgag
    11041 taacccttat acaccgtggg tttatcttcg atatcgtcat ctaatagcca aaaatcaacg
    11101 ggtaggatta ccctgagcct ataaaattct ctcctctgta tcctttggat tggggaagtc
    11161 cgtgtaatac taaccaccac agcattatta attctgaatc ttttttttac tctaccatgg
    11221 aatagaaagc tggcttcttc tttgaaaaaa tatatttc
//
LOCUS       DASRNX010000128         6049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_502420,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000128 DASRNX010000000
VERSION     DASRNX010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502420"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..610
                     /locus_tag="VFD89_05195"
     CDS             158..610
                     /locus_tag="VFD89_05195"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HZJ57620.1"
                     /translation="MKEMGQIVHVEDSIATVKIRRGVSCGSCTACGMSKDQSEISFSI
                     ANDLDAQLGDWVELDLESKSLVKASFIVYLIPLIALILGVAGGYAIAGQCSGDSDLYG
                     AIGGILFTVFAFVGIRAMDPMLRKRGEYSPKMVSIISLYSKGEIIDGE"
     gene            600..929
                     /gene="trxA"
                     /locus_tag="VFD89_05200"
     CDS             600..929
                     /gene="trxA"
                     /locus_tag="VFD89_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZJ57621.1"
                     /translation="MANEKIITLNSNNFEDEVMNSDVPVLVDFWAAWCGPCRMIAPII
                     DQLADDFDGKAKIAKVNVDEERELASRYRVMSIPTLILFKDGQIADQTMGARPKSDLV
                     KMIQGAL"
     gene            1075..2325
                     /gene="glyA"
                     /locus_tag="VFD89_05205"
     CDS             1075..2325
                     /gene="glyA"
                     /locus_tag="VFD89_05205"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZJ57622.1"
                     /translation="MINLKEVHKTDPEVAEAMEQELHRQQSHIELIASENFVSPAVMD
                     AMGSHLTNKYAEGYPGRRYYGGCEYVDIVEELAINRAKELFGAEYANVQPHSGAQANM
                     AVYFAFLEPGDTILGMNLAHGGHLTHGSPVNISGKYFNIVPYGVDSETGYIDYKELEN
                     LAKEHRPKLIVAGASAYSRIIDFETFGQIAREVGAYLMVDMAHIAGLVAADLHPNPVP
                     FADFVTTTTHKTLRGPRGGLILCKEKYGRAIDKAIFPGTQGGPLMHVIAAKAVCFLEA
                     LKPEFKEYQREIANNAKALAEALKDEGFQLVSGGTDNHLMTIDLRNKGITGKDAEKIL
                     DDVKITTNKNTIPNDPQSPFVTSGVRIGTPAVTTRGMGTEEMKAIAKAIALAIDDVEA
                     NRAEILNIVDTLCINFPLYSDGVL"
     gene            2444..3736
                     /locus_tag="VFD89_05210"
     CDS             2444..3736
                     /locus_tag="VFD89_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZJ57623.1"
                     /translation="MIKNMPLAARMRPETLNEFIGQKHIVGRNKLLYRAIKADRLTSV
                     IFYGPPGTGKTTLAKIIAGETQYEFKQLNAVSAGVKDIRESADEAQNYLLNPKGRMIL
                     FFDEIHRLNKNQQDALLPYVENGTFILIGATTENPYFEVNKALLSRSTIFKFNPLTDQ
                     DLRDLVQNCIHDEKKGLGNYKISLTPGATDHIIRMSEGDARSVLNALELAVLTTPTNH
                     EGIIDISLEVAQECVQQKAMRYDKDGDNHYDVVSAFIKSMRNYMEPDAVLHWLARMIE
                     SGEKPEFIARRIIIAAAEDVGMANPAALQVAVAASQAVERVGWPESRIILAQAALMVA
                     CSPKSNAVCVGIDKALEDVRNKKIGEVPANLKDTHYPGAKEQGHGKGYKYSHDYPGGR
                     AIQQYLPEELKDARYYLPKNSGTEARIKDRMDQILKDR"
     gene            3837..4313
                     /locus_tag="VFD89_05215"
     CDS             3837..4313
                     /locus_tag="VFD89_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZJ57624.1"
                     /translation="MKLSTRGRYGLRAMFDLAMNESQRPVPLNEIAGRQNISESYLEQ
                     LFAALRRQGLVSSVRGAHGGYLLGSSPDEIYIGQILRTLEGDIAPADCVSDEDIVGCM
                     DADACVTRIIWEKIRDSINQVIDSITLEDMVRQQKLINNRRDSASPEDSWVIRCNN"
     gene            4393..5580
                     /gene="nifS"
                     /locus_tag="VFD89_05220"
     CDS             4393..5580
                     /gene="nifS"
                     /locus_tag="VFD89_05220"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HZJ57625.1"
                     /translation="MTKNIYLDHAATTFTKKEVLNAMLPYYTQDYGNPSSIHTFGRQA
                     QRAVDEARERTSKVLNAKFGEIFFTGGGTEADNWAIKGVALANMDRGRHIITSSVEHH
                     AVLYTCEFLEKMGFEVTYLPVDRYGMVDPSDIEKSIRQDTILVSVMFANNEIGTVQPI
                     REIGGITQKKGVYFHTDAVQAIGCADIDVNEMNIDLLSLSAHKFYGPKGIGALFIRKG
                     TRITPLQHGGAQERNQRAGTENIPGIVGLGKAIELAHTNMKENNKRLLRLRDRLIGGI
                     MDRIDNVILNGHPVDRLPGNINLSFEYIEGESLLLSLDMKGIAASSGSACTSGSLDPS
                     HVLLAIGLSHEIAHGSLRITLGDDNTEGDVDYVLEVLPPIVKRLREMSPLFVQEKGGC
                     HYV"
     gene            5573..5956
                     /gene="nifU"
                     /locus_tag="VFD89_05225"
     CDS             5573..5956
                     /gene="nifU"
                     /locus_tag="VFD89_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HZJ57626.1"
                     /translation="MYSDKVMEHFANPRNVGELNNPDGVGEVGNARCGDIMRIYLKVE
                     NDVIVDVKFKTFGCGAAIASSSMATELIKGKTIDEALLITNKAVTQALDGLPPVKLHC
                     SVLAEEAIQKAIEDYREKQKSVVGA"
BASE COUNT         1831 a         1143 c         1541 g         1534 t
ORIGIN      
        1 gttaactata aacttatagt gtgcccggat aggaagggaa tcccttgttt taatcccatt
       61 ctattatccg aatatgcaat ggatctttta tagcggtttt aagactggcc ttgctatgtt
      121 ttcaaagcga taaatttgta cggtaaggat gatgtcaatg aaggaaatgg ggcaaattgt
      181 ccatgtagaa gatagcatcg caactgtcaa gatacgaagg ggtgtcagct gcggatcctg
      241 tactgcctgt ggtatgagta aggaccaaag tgaaatatcc tttagcatag ccaacgatct
      301 ggatgctcaa cttggggatt gggtagaact ggatttagaa tcaaaatccc tcgtcaaggc
      361 ctccttcata gtatacctta tacctttaat tgcccttatc ctgggggttg ccggaggcta
      421 tgccatcgcc gggcaatgct ccggcgactc cgacctgtat ggggcaatag gcggaatact
      481 ttttactgtc tttgcctttg tgggcataag ggctatggat ccgatgctta ggaaaagggg
      541 ggaatactcc cccaagatgg tatcaattat tagtttatat tcgaaagggg aaattatcga
      601 tggcgaatga aaaaatcata acgcttaatt ccaacaactt tgaggacgaa gtaatgaatt
      661 ccgatgtacc cgtcttggta gacttttggg cggcttggtg tggtccttgc aggatgatcg
      721 ctccaatcat tgaccaactg gctgacgact ttgacggcaa ggcaaagata gccaaggtaa
      781 atgtagacga ggagagggag ctggcatcaa ggtatagggt gatgagcatc ccaaccttga
      841 ttctattcaa ggatggtcag atagcggatc aaaccatggg tgcgcggcct aaatcggact
      901 tggttaagat gatacaaggt gcattataaa aagaagctgg atttatacca gcttttttta
      961 taccgtaatt ttctatgaaa accttgtaaa ttatatgaaa tctaactata atgtaatcta
     1021 ttgaaaccga acaatttaaa taattataac gaaaacgttt ggaggaacca aattatgatc
     1081 aacttgaagg aagttcataa aaccgacccg gaagttgccg aggctatgga gcaggaatta
     1141 cacaggcaac aaagccatat agaacttata gcttctgaaa attttgttag cccggcggtt
     1201 atggatgcta tgggctctca tctgacaaat aaatatgcag agggatatcc gggcaggaga
     1261 tactatggtg gctgcgagta tgtagatata gttgaagaat tggccattaa cagggcaaag
     1321 gaactcttcg gtgcagaata tgccaatgtt cagccccact ccggcgcaca ggccaatatg
     1381 gccgtatact ttgccttctt agaacccggg gatactattt tgggaatgaa tctggcccat
     1441 ggcgggcatt tgacccatgg cagtcccgtg aatatttcgg gtaagtattt caatattgta
     1501 ccgtatgggg tggattcaga aacggggtat atagattata aagagctcga aaatctggcc
     1561 aaagagcata gacctaagct aatagtggca ggggccagtg cctactcaag aattatcgac
     1621 tttgaaacat ttggtcaaat cgcccgtgaa gtgggagcat atctaatggt ggatatggcc
     1681 catattgcgg gtttagtagc agcagaccta cacccaaatc cggtaccctt cgcagacttc
     1741 gtgacgacca ccacccataa aacccttagg ggtcctaggg gtggcttaat cctatgcaaa
     1801 gagaaatatg gcagggcaat agacaaagca attttcccgg gtacccaagg cgggccgttg
     1861 atgcacgtga ttgccgccaa agcagtttgc tttcttgagg cattgaaacc ggaatttaaa
     1921 gagtatcaga gggagatagc caacaatgct aaggctcttg cagaggcttt aaaggatgag
     1981 ggattccaat tggtatccgg gggtacagac aatcatctta tgaccattga tcttaggaat
     2041 aaaggtataa cgggtaaaga tgccgaaaaa atattagatg atgtaaagat tactaccaat
     2101 aaaaacacca tccccaatga cccccagagc ccctttgtta caagcggggt acgaatagga
     2161 actcctgccg tgaccactag ggggatgggg acagaagaaa tgaaagccat cgccaaggcc
     2221 atcgccctgg ctattgatga tgtggaagct aatagggccg aaatcttgaa tatagtagat
     2281 accctatgta ttaactttcc gctgtattct gatggagttt tatgattttt ttaccgacag
     2341 gacagcctta tcttgtagca ggggggattg ggaagtatcc taccgggaag ccagttaccc
     2401 ccattaaggc tttctattac aataagcgtc aaggaggata ataatgataa aaaatatgcc
     2461 cctggcggct cgcatgagac ctgaaacttt gaatgagttt atcggccaga agcatatcgt
     2521 aggcaggaat aaattattgt atagggcaat aaaggcagac cggttaacct ctgttatatt
     2581 ctatggacca ccgggcacgg gtaaaactac cctggctaag attatagccg gtgagacaca
     2641 atatgaattc aagcagctaa atgccgtatc agccggtgtg aaggatattc gtgaatcggc
     2701 cgacgaggcc cagaattatc tattaaaccc gaagggacgg atgattctat tttttgatga
     2761 gatccacagg ctaaacaaaa atcagcagga tgccctgctc ccctacgtgg agaacgggac
     2821 atttatactc attggagcga caacggagaa cccatatttt gaagtaaata aggccttgtt
     2881 atcgagatcc accattttca aatttaatcc tctaacagat caagacctga gggacttagt
     2941 gcaaaactgt atccatgatg agaaaaaggg tttgggtaac tataaaatat cattgacccc
     3001 gggggcaacg gatcatatta tacgcatgag tgagggggat gcgaggtcgg tgttgaatgc
     3061 gctggaattg gcggtcctga ccacccccac caaccatgag ggtatcatag atatttcact
     3121 agaggtggcc caggaatgcg tacagcaaaa ggctatgagg tatgataagg acggggataa
     3181 tcattatgat gtggtaagcg ccttcattaa aagcatgagg aactatatgg aacccgatgc
     3241 ggtgttgcat tggttggcgc gtatgataga gagcggggaa aaacccgaat ttatcgcaag
     3301 aaggataatt attgctgcgg ccgaggatgt ggggatggcc aatcccgcgg ccctacaagt
     3361 agcagtggcg gcttcccaag cggttgaaag ggttggatgg cccgaaagca ggattattct
     3421 ggctcaggcc gcgttgatgg tggcatgctc gccgaaatca aacgcagtgt gtgtgggtat
     3481 agataaggcg ttggaggatg tgcgaaacaa aaagattggg gaagtgcccg caaatctaaa
     3541 agacacccac tatccgggcg ctaaagaaca gggccatggg aaaggatata agtattccca
     3601 tgactatccg gggggcaggg caatacaaca atacttaccg gaggaactca aagatgccag
     3661 atattacctc cctaaaaatt ccggtactga ggccaggata aaggatcgca tggatcaaat
     3721 ccttaaagac agataaaaaa tagtggctga tttaatgttg acaaaaatag tcgggtatga
     3781 tattattttt ttgtaacaaa gcatagtaat ttagtcggga ttcggaggtg tcgattttga
     3841 aactttctac acggggtagg tatggcctta gggcaatgtt cgatctggcc atgaatgaat
     3901 cccaaaggcc tgtcccgctg aacgaaatag ccggtaggca gaatatctca gaatcatact
     3961 tagaacagtt atttgctgcc ctgaggagac agggattggt tagtagcgtt cgtggggccc
     4021 atggtggcta cttattaggc tcatcccccg atgagatcta tatagggcag atactaagaa
     4081 ccctggaagg agatattgcg ccggctgatt gtgttagtga tgaggatata gtaggatgca
     4141 tggatgctga tgcttgcgtt accagaatta tttgggagaa gataagggac agtataaatc
     4201 aagtgataga ctccataact ttggaggata tggtacgcca acaaaagctg attaataaca
     4261 gaagggattc agcttctcct gaagacagct gggttatacg atgcaataat tagagcagga
     4321 aacaattatt aaaggggtga atatgggtgt ctcccgtgta atcgctactc gggttttaca
     4381 tccagatatc ttatgacgaa gaatatttac ttagatcatg ccgcaactac tttcacaaaa
     4441 aaggaagtcc tgaatgcaat gttaccctat tatacccagg attatggaaa tccctccagc
     4501 atccatacct tcggaagaca ggcccaaagg gctgttgatg aggctaggga aaggacatcc
     4561 aaggtattaa atgctaagtt tggggagata ttttttaccg gaggcggtac ggaggccgac
     4621 aactgggcca taaagggcgt tgccttagct aatatggata gggggaggca tataataaca
     4681 tcctctgttg aacaccatgc cgttttgtat acctgcgaat ttttagaaaa gatgggcttt
     4741 gaagttacct atctccctgt agatagatac ggaatggttg acccatctga tatagaaaag
     4801 tccatcagac aggatactat tcttgtttcg gtgatgttcg ccaataacga aattggtact
     4861 gtccaaccta taagggaaat tgggggaata acccaaaaga agggtgtata ttttcatacc
     4921 gatgcggttc aggctatagg ttgcgcagat attgatgtaa acgaaatgaa tattgatcta
     4981 ttgtccctat cagcccataa gttctatggt cccaagggaa ttggggcatt gttcataaga
     5041 aagggaacta ggataacccc ccttcaacat ggcggggccc aagaacgtaa ccagagggct
     5101 gggacagaaa acataccggg tattgtaggt cttggaaagg ccatagagtt agcccatacc
     5161 aatatgaagg aaaacaataa acgcttatta aggctaaggg acaggcttat tggcggcatt
     5221 atggatagga tagataatgt catattaaat ggacaccctg ttgataggct tcccggcaat
     5281 ataaatttaa gttttgaata tatagaaggt gaatctctgc tccttagtct cgacatgaag
     5341 ggtattgcag cttccagtgg atctgcatgt acatcaggat cattagatcc gtcccatgta
     5401 cttttggcaa tcggtctatc ccatgaaatt gcccatggct ccttgaggat tacattaggg
     5461 gatgacaaca cagaagggga tgtagattat gtactcgagg tattgccgcc tatagtaaag
     5521 aggctcaggg agatgtcgcc cttatttgta caagaaaaag gaggatgcca ttatgtatag
     5581 tgataaggta atggaacatt ttgcaaaccc acgaaatgta ggggaattga ataatcccga
     5641 tggggtgggg gaagtgggca acgcaagatg cggggatata atgaggatat acctaaaggt
     5701 ggaaaatgac gtcatcgtag atgtcaaatt taagaccttt ggctgcggtg ctgcaattgc
     5761 cagcagtagc atggccacag aattaatcaa aggcaagacc atagacgagg ccttgcttat
     5821 aaccaataaa gcggtcaccc aagcactgga cggattgccg ccggtaaagc tgcattgctc
     5881 tgttttagcg gaggaggcaa tacaaaaagc tattgaagat tatagggaaa aacagaaaag
     5941 tgtagttggt gcataattga gacccctagt gatgggtatt aaattatcct tggttttttt
     6001 ggtgttactt tccatgatct cttatctcaa ttataggact atatgaata
//
LOCUS       DASRNX010000129         7539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_608224,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000129 DASRNX010000000
VERSION     DASRNX010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608224"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFD89_05230"
     CDS             complement(<1..219)
                     /locus_tag="VFD89_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HZJ57627.1"
                     /translation="MKKVKLILYFPPEKTDKPFSYYLVKDYDLIFNILQAEVQPGKRG
                     RLTVELEGTEENIELGLAFAEKNGVQYRI"
     gene            complement(221..1381)
                     /locus_tag="VFD89_05235"
     CDS             complement(221..1381)
                     /locus_tag="VFD89_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HZJ57628.1"
                     /translation="MGKTFDEINEKIRSGDVVVATAEEIIEIAEEKGVKEAAKSVDVV
                     TTATFGPMCSTGAFLNFGHSDPPIRMNKVWLNDVPAYGGIAAVDAYIGATELSESRGL
                     EYGGAHVIEELVDGKKVRLTAKSYGTDCYPRLDIDTFITLEDLNQAYLFNPRNVYQNY
                     SAATNTSDKIIDTYMGTLLPRCGNITYSTSGELSPLLNDPEFRTIGIGTRIFLGGVQG
                     YIAWEGTQFVRNREEIDDGITMYSGGTLAVIGDLKGMTSRYLRAASFARYGTTMFMGI
                     GIPIPVLDEETMEYLLVTNKDIYTEVVDYSVPRRSKPTLRKVNYEELRSGSVELKGKK
                     VPTAPLSSLNRAREIANILKESIEAGEFFLQQPVQNMPAHRGFNPLKDVGVE"
     gene            complement(1403..3280)
                     /gene="htpG"
                     /locus_tag="VFD89_05240"
     CDS             complement(1403..3280)
                     /gene="htpG"
                     /locus_tag="VFD89_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HZJ57629.1"
                     /translation="MFKESGNIAVHTENIFPIIKKWLYSDKDIFLRELVSNGSDAIQK
                     LKRLSDIGQVDIGGQSFFKVEVVLDKEEGTIKVMDNGIGMTRDEIKKYINQVAFSSAQ
                     EFLEKYKDQPGEGSQIIGHFGLGFYSAFMVAEKVQIDTLSWEEGAEAVRWTSETGLDY
                     SMEVSDSRTQRGTTITLHISEDSKEFLEGYRLREILNKYCSFLPVEIYFEDTKDKKDE
                     DGEDPKPINDVRPLWLKHPNDCTDEEYKEFYQKVFHDFTDPLFWVHLNVDYPFNLKGI
                     LYFPRLNNQFETLEGQIKLFSNQVFVADNIKEVIPEFLMLLKGTIDCPDLPLNVSRSF
                     LQNDGNVQKISSYISRKVADKLSSLFKNEREDYNKYWDDINPFIKYGCMRDQKFYDQV
                     KDFVIFKTLDGEYTTLKDYLDKNKERHENQVYYVNDETQQAQYIRLFREHDLDAVYLN
                     NIIDTHFISFLEMNEGSVKFKRVDSDISESLKDIQEKGDKKADKKTDKALEKLFKEAI
                     DKDDIKIEVEGLKNRDIPAMVLLSEESRRMEEMAQRFGNMGMNYPMPKEETLIVNRNS
                     DLVESLVKLSGDESRGDDLKVICRHIYDLALVAHKPLGSEEMMEFIQRGNKILTRLAG
                     L"
     gene            complement(3472..3984)
                     /locus_tag="VFD89_05245"
     CDS             complement(3472..3984)
                     /locus_tag="VFD89_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57630.1"
                     /translation="MKGTQGYFWGIFLIGIGILMILKYQFKLNISMPRIIIGVLFLSL
                     GISMLLGGPWVNTQSNIIFSESKVQAVKPEKEYNVIFGNLVIDLSDISEDELGDTIKI
                     NTVFGNALVKIDPNIPTLIKMTSAFGSGSTPDGSTVTFGDHTHRTGEGDRPLTIEANV
                     VFGKIDIIEN"
     gene            complement(4195..5601)
                     /gene="uxaC"
                     /locus_tag="VFD89_05250"
     CDS             complement(4195..5601)
                     /gene="uxaC"
                     /locus_tag="VFD89_05250"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HZJ57631.1"
                     /translation="MERFMDESFLLSNKTAAKLYHDYARDMPIYDYHCHLNPVEIWED
                     KGYDNITQVWLYGDHYKWRAMRANGVDEKYITGDGSDYEKFVAWAKTVPYTIGNPLYH
                     WTHLELRRYFDINEVLDGNTADLIWNKANEKLGQDGFTARGFIQRSNVRLLCTTDDPT
                     DDLVYHRKIEDLQGFDVKVLPAFRPDKGVEIQNKAFSCWVNKLGNTAGMGIHSFGDYL
                     MALEDRVDYFHDRGCRLSDHGLEYVPYRRTDDEELDAIFVKGRKGIEITREEEESFKT
                     AVLLFLGRLYAEKNWAMQLHIGAIRNNNTRMFGLLGPDTGFDSIHDHNIAYGLSRFLD
                     ALESAEMLPRTILYTLNPKDNYVLASMAGNFQGGKIPGKVQFGSAWWFNDQRDGMVEQ
                     MRALANIGLLSRFVGMLTDSRSFLSYTRHEYFRRILCNIVGKWAEDGEVPNDMALLGR
                     MVEDICFNNAADYFQMEL"
     gene            6276..7145
                     /locus_tag="VFD89_05255"
     CDS             6276..7145
                     /locus_tag="VFD89_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZJ57632.1"
                     /translation="MTLKVGIQLYSVRQTLKKKPFETLEKVAAAGYRYAEAANHDAGN
                     DPGVGFGLSAKEMKNALDNLGLSIVGCHINPISIDRLPAILDYHQELGNRQIGCDIEF
                     YPYEDMDYLLRRCEFFNEVGEMCKERGMRYYYHNHYQEFQKFGDKYVYDIIMENTDPD
                     LVFTEMDTYWIMRSGQDPIKVIEKYGDRLILLHQKDFPKDAPQPVVMYDGIIHKEKDI
                     TYPMFEDTIDPRCFTEIGTGIMPIQDIIDAASKTPNLEYVILEQDHTKYDEIESINIS
                     MRSFQKYSGIEVG"
     gene            complement(7295..7371)
                     /locus_tag="VFD89_05260"
     tRNA            complement(7295..7371)
                     /locus_tag="VFD89_05260"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7335..7337),aa:Arg,seq:acg)
BASE COUNT         2020 a         1818 c         1408 g         2293 t
ORIGIN      
        1 gatccggtat tgcaccccgt ttttctcggc gaaggccagg cccaactcta tattctcttc
       61 ggtcccttcc agctcaacgg ttagcctgcc cctcttaccc ggctgtacct ctgcctgtaa
      121 gatattgaat attaggtcat aatcctttac caagtaatag gagaaaggtt tgtcggtctt
      181 ttcaggaggg aaatacagga tcagctttac ctttttcatg ctattccacc cccacgtcct
      241 ttaatggatt aaatccccta tgcgccggca tattttgcac aggctgttgg aggaaaaact
      301 cacccgcttc tatgctctct tttaagatat tggctatctc ccttgccctg ttgaggctcg
      361 ataggggagc tgtcggtacc tttttgccct ttaattcaac actgccgctt ctcagctcct
      421 cgtagtttac cttccttaaa gttggcttag agcgcctagg aacactatag tccactacct
      481 ccgtatagat atccttgttg gtaaccaaca aatactccat cgtctcttcg tccaagaccg
      541 gtatgggaat acctataccc ataaacatgg tggtgccgta tctggcaaag ctggcagccc
      601 tcaagtatct ggaggtcatc ccctttaaat caccgattac cgccagggtc cccccagaat
      661 acatggtgat accgtcgtcg atctcttccc tgtttcttac gaactgggtc ccttcccagg
      721 cgatataacc ctgaaccccg cctaggaata ttctggtgcc tatacctatg gttctaaatt
      781 ccggatcgtt cagcagcggg ctcagctctc cggaagtaga ataggtaata tttccgcatc
      841 tcggaagcag ggtccccata taggtatcta taatcttatc cgacgtattg gttgcggcgc
      901 tatagttttg atagacattt ctcggattaa acaaataggc ctggtttaaa tcctctaaag
      961 ttataaaagt atctatatcc aatcggggat agcagtcggt cccataggat ttggcggtca
     1021 atctgacttt tttcccgtcc accaattctt cgatgacgtg ggcaccccca tactcaaggc
     1081 ctctactctc cgacagctcg gtagccccta tataggcatc caccgcggct atgccgccgt
     1141 atgccggcac atcgtttaac cataccttat tcatcctaat aggcggatcg gaatgcccaa
     1201 agtttaaaaa tgcaccggtg gagcacatgg gcccaaaggt agccgtggtt accacatcca
     1261 cacttttcgc ggcctccttg acaccctttt cttccgctat ctcaatgatc tcctcggccg
     1321 ttgccaccac aacatcgccg cttcggatct tttcgtttat ctcatcaaag gttttaccca
     1381 tgggaacctc tcccttcctt cgctataagc ctgcaagtct cgttaagatt ttatttcccc
     1441 tctggatgaa ttccatcatc tcctcagagc ctagaggttt atgagccaca agggccagat
     1501 catatatgtg cctgcatatc accttaagat cgtcacccct ggattcatcc cctgataatt
     1561 tcacaaggga ttccaccagg tcactgtttc tgttgactat cagagtctct tccttaggca
     1621 tgggataatt catccccata ttcccgaatc tttgggccat ctcctccatc cttctggatt
     1681 cctcggacaa aaggaccata gccgggatgt ctctgttctt caggccctct acctcaattt
     1741 taatgtcatc tttatctatt gcctctttaa ataacttttc cagcgcctta tcggtcttct
     1801 tgtctgcctt tttatcgccc ttttcctgta tatcctttag actttcggat atatccgaat
     1861 ctaccctttt aaatttaaca gatccctcgt tcatctccag gaaagagata aaatgggtat
     1921 ctattatatt gtttaaatac accgcatcta aatcgtgttc cctaaatagc cttatatact
     1981 gggcctgctg ggtttcgtca tttacataat agacctggtt ttcatgcctt tccttgttct
     2041 tatccagata gtcctttaag gttgtatact ccccatccaa agtcttaaag atgacaaagt
     2101 cttttacctg gtcatagaac ttctgatccc tcatgcagcc atatttaatg aaggggttta
     2161 tatcgtccca atacttgtta taatcttccc tttcgttttt aaacagtgat gacagcttat
     2221 ccgctacctt tctggatata taggaagaga tcttctgaac attcccatcg ttttgcagga
     2281 agctccggga tacgtttagg ggtaaatcgg gacaatctat agtccccttt aaaagcatca
     2341 aaaattccgg tatcacttcc tttatgttat ccgccacgaa cacctggttg ctaaagagct
     2401 ttatctgacc ctctaaggtc tcgaactgat tatttaaacg ggggaaatat aaaattccct
     2461 taaggttgaa gggatagtcc acgtttagat gaacccaaaa taacgggtcc gtgaagtcat
     2521 ggaacacctt ctggtaaaat tccttatact cctcgtcggt gcagtcgtta ggatgcttaa
     2581 gccatagggg acgtacatcg tttatgggtt ttggatcttc cccgtcctca tctttcttgt
     2641 ccttcgtatc ctcgaagtag atctctacag ggaggaagct acaatattta tttaggattt
     2701 ccctgagcct atacccttcc aagaactcct tgctgtcctc cgatatatga agggtaatag
     2761 tggttcccct ttgtgtcctg ctgtctgaaa cctccatact ataatccagc ccggtttcgc
     2821 tggtccaacg caccgcttct gccccttctt cccatgaaag ggtatcgatt tgaacttttt
     2881 cagccaccat gaatgccgaa taaaagccta ggccaaaatg tcctattatt tggctaccct
     2941 ccccaggttg gtccttatat ttctccaaaa actcttgggc gctggagaat gccacctggt
     3001 ttatatactt ttttatctca tccctggtca tgcctatacc gttatccatg accttgatgg
     3061 ttccctcttc cttatccaat accacctcta ccttgaaaaa ggattgaccc ccaatgtcca
     3121 cctgccctat atccgatagc ctcttaagct tttgaatggc atcgctaccg tttgatacca
     3181 gctctctgag aaaaatatcc ttatccgagt atagccattt tttaataatc gggaaaatat
     3241 tctccgtatg tactgcaata tttccactct ctttgaacat atataatcct cctggttttt
     3301 tggtctcttt ttattttaca ccagatgctt ttgttgtcaa gaaattggga taccaaaacc
     3361 cagatttaat gggttttggt atctatagat aaacaatacg gaaactaaat ttatcactag
     3421 ttgataaatt ttatagttgg aattgtttat ctctggatgt gtctataatc cctaattttc
     3481 tatgatatca attttgccaa agaccacatt ggcctcgatg gtaaggggcc gatccccttc
     3541 tccggtcctg tgggtatgat ccccgaatgt tacagtcgat ccgtcgggag tgctcccgct
     3601 gccaaaggca gaggtcatct taattaatgt aggaatattg ggatcaatct ttaccagtgc
     3661 gtttccaaac actgtattaa ttttaatggt atcccccagc tcatcctcgg atatgtcaga
     3721 taggtcaata accagattcc caaatattac gttgtattcc ttctcaggtt tcaccgcctg
     3781 gaccttgctt tccgaaaaga taatgttaga ttgggtattt acccacggtc cgcccagcag
     3841 catagatatc cccaacgaca ggaaaagaac cccgataatt attctcggca tagaaatatt
     3901 aagtttgaat tgatatttta ggatcatcag tattcctata ccaattaaga aaatgcccca
     3961 aaaataccct tgtgtacctt tcatcctctc atccccctta tttttaataa aatgacccga
     4021 cacgcaggct actatagata aacaatacgg aaactaaatt catcggcacg ataattgcat
     4081 cggttctatg tcatctcatg atgataattt ttatagctaa agtcctttat ctgccgacat
     4141 ctaacccatt gactatataa tgtggaaggt tttaaatacc tgaaataacc gccgctacag
     4201 ctccatttgg aaatagtccg ccgcattgtt gaagcaaatg tcctccacca ttctcccaag
     4261 caaggccata tcgttcggta cttccccatc ctctgcccat ttgcctacga tattacatag
     4321 aattcttcgg aagtattcgt gtcttgtgta ggacaaaaag ctcctagagt ccgtgagcat
     4381 cccaacaaat cggctgagta atccaatatt ggccagggct ctcatctgct ctaccatgcc
     4441 atccctttgg tcattgaacc accatgccga cccgaactga acctttccgg gaattttccc
     4501 gccttggaag ttgcccgcca tagaggccaa tacatagtta tccttgggat ttagggtata
     4561 caatatagtc cttgggagca tctccgcact ttctaaggca tctaaaaagc gggacagtcc
     4621 ataggcgata ttatgatcat ggatggaatc aaagcccgta tcaggaccca gcaacccaaa
     4681 catccttgta ttgttatttc ttatggcgcc aatatgaagc tgcatagccc agttcttttc
     4741 ggcatacaac ctgcctaaaa ataatagaac ggcagtttta aaggattctt cctcttccct
     4801 ggttatttca atccccttgc gccctttcac gaagattgca tccagctctt catcgtcggt
     4861 ccttctgtag ggaacgtatt caagcccgtg gtccgacagc cggcaacccc tatcgtgaaa
     4921 gtagtcaacc ctatcttcca gtgccattaa atagtcccca aaagaatgga tacccattcc
     4981 ggcagtattc cccagtttat taacccaaca gctaaaggcc ttgttttgga tttctacacc
     5041 cttatcgggc ctgaatgcgg gcaaaacttt aacatcaaag ccttgcagat cttcaatttt
     5101 tctatggtac acaagatcgt ctgtaggatc atctgtagtg caaagaagcc ttacattgga
     5161 cctttgtatg aatcccctgg cggtaaagcc atcctgcccc agcttttcat tagccttatt
     5221 ccagatcaga tcggctgtgt tgccatctaa gacctcgttt atatcaaaat atcttctaag
     5281 ctccaaatgc gtccaatggt atagggggtt tcctatggta tatgggacgg ttttggccca
     5341 ggctacaaat ttttcataat cgcttccgtc cccggttatg tacttttcgt ctacaccgtt
     5401 ggctctcatg gccctccact tataatggtc tccatagagc catacctgag ttatattgtc
     5461 atagcctttg tcttcccata tttccacggg attcaagtgg caatggtagt cgtagatagg
     5521 catatctcta gcgtaatcat gatataattt ggcagctgtt ttatttgaca gcagaaaact
     5581 ttcgtccata aatctctcca tcgttctcac cttccgtcta ggattcttta aaagacacga
     5641 taaacaaaaa gtatcaataa caatttacca cccattggca atttaatcaa acataatttt
     5701 ttacagatga tctttccaca ttattcacag cttgaactac cacttataca cagcaattaa
     5761 cccaatatta ttggatatca tcaataatat ccacaatttt atctacaggt gtgtatggat
     5821 ttgcctgatt ttttttggat tatcgctata aggaaagatt ttatccacag aaattcatgt
     5881 tttctatcgt tttgtggata actttgtgga caacccttca ttttttgtgg aatatgttaa
     5941 gttatccaca ttcttattcc agcatggaac ttttcttgca ttaatccaca aaacccacta
     6001 tcattagtaa tgaatccgct gcaagatgtg tgaatatttc ggccccatgg cgggttattc
     6061 tgtaggatat ataacgagtc gccaagatgt ctcccgcttc tataagcggc gagttcccat
     6121 tagtaaaaca ggcggtgggt ataaatttcc cgactcgttg aaacctgtgt gttgcaattg
     6181 ccaatgcaat tgcttccggt gttttaatat aatgaacgca ggatgtgtcc ctacacatcc
     6241 tttttaaaaa aactattgat ataaggagca attttatgac cctaaaggta ggaattcagc
     6301 tttattcagt acgtcaaaca ctaaagaaga agccctttga gaccttggaa aaggttgcgg
     6361 cagcaggata tcgatatgcc gaagcggcaa atcacgatgc aggaaatgac cccggcgtcg
     6421 gatttggatt gtctgcaaag gaaatgaaaa acgccctaga taacttgggc ttatccatcg
     6481 tgggttgcca tattaaccct ataagcattg atcgcctccc ggccatctta gattatcacc
     6541 aggagttggg gaataggcaa atcggctgtg atattgaatt ctatccctac gaggatatgg
     6601 attatttgct ccgcagatgt gaattcttta atgaggttgg tgagatgtgc aaagaacgcg
     6661 gtatgcgcta ctactatcat aaccactatc aggagttcca aaaatttggt gataaatatg
     6721 tttacgatat tattatggaa aatacagatc cggacctggt tttcaccgaa atggatacct
     6781 attggatcat gaggagcggc caggacccca taaaagttat agaaaaatat ggggatcgct
     6841 tgattcttct ccatcaaaag gattttccaa aagatgcgcc acaacccgtt gttatgtatg
     6901 atgggattat ccataaggaa aaggatatta catatcccat gtttgaggac accatagatc
     6961 caagatgctt tacagaaatc ggtaccggaa tcatgcctat ccaagatatt atagatgctg
     7021 cgtcaaagac accgaacctt gaatatgtta ttttagagca ggatcataca aaatatgacg
     7081 aaattgaatc cattaatatc agtatgaggt catttcaaaa gtattcaggt attgaagtcg
     7141 gctaaaaaac ggatggatga ctcaatctga aaactactct agatactgcg tcaagcatag
     7201 catagccatc tttacagata ctacgaggtt actacgtatc caaaaataat aaaaccgcat
     7261 gaaccgggaa tatccggcgt tcaagcggtt ttattggtat acccggaggg agtcgaaccc
     7321 ccgacctctt gattcgtagt caagcgctct atccagctga gctacgggta catatgcgtc
     7381 tatcgggtca aaagactgaa tattattgta ttacaatgga cttaaagtgt caagtattac
     7441 attttgggaa aaaaattgac ccaaatttaa ctgggtcaaa aaagtatata ataaatataa
     7501 ggggagttaa caaaaaatta accctatcaa ttatagcac
//
LOCUS       DASRNX010000130        16191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_502804,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000130 DASRNX010000000
VERSION     DASRNX010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 16191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16191
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502804"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /gene="pstB"
                     /locus_tag="VFD89_05265"
     CDS             <1..718
                     /gene="pstB"
                     /locus_tag="VFD89_05265"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZJ57633.1"
                     /translation="YYGDFQALKNINMSIPANEITAFIGPSGCGKSTLLKSLNRMNDL
                     IEGCRIDGEVFLDDTNIYENIDVNLLRKRVGMVFQKPNPFPMSVYDNVAYGPRTHGIK
                     SKAKLDDIVERSLTDAAIWNDLKDRLKSSALSLSGGQQQRLCIARALAVEPEVLLMDE
                     PTSALDPISTSKIEDLALELKKKYTIVMVTHNMQQATRISDKTAFFLLGEIIEMDETE
                     KLFANPGDKRTEDYITGRFG"
     gene            718..1362
                     /gene="phoU"
                     /locus_tag="VFD89_05270"
     CDS             718..1362
                     /gene="phoU"
                     /locus_tag="VFD89_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZJ57634.1"
                     /translation="MRSRFDAQLDILNNSLISMGALCENAIASAVKALLHNDMSLSKK
                     AIETEVELDQMEKDIERLCLRLLLQHHPVAGDLRLISAALKMITDMERIGDQAADIAE
                     IVAYVDLTDNINKVRIADMAKATIKMVTESIDAFVKRDLKVAEDVIDYDDVVDDLFNK
                     IKDDAIALITENPEYGEQFVDIVMIAKYLERIGDHATNIAEWVVFSITGEHKEQ"
     gene            1364..2038
                     /locus_tag="VFD89_05275"
     CDS             1364..2038
                     /locus_tag="VFD89_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZJ57635.1"
                     /translation="MIYYVEDDEGIRELVVYTLQNTGFQAVGFENGQQLFSALQNTKP
                     QLILLDIMLPGDDGMTILRRLRSDIATRKIPVIMITAKGSEYDKVIGLDSGADDYVTK
                     PFGMMELVSRIRAVLRRTGVVENHEVYSYKSVVLDTRKHSVTVKDRVVDLTLKEFELL
                     KLLMKNAGAVLTRDMLLENIWGYDFDGETRTVDVHIRTLRQKLSDEGKIIETVRGVGY
                     RLGGTE"
     gene            2039..3700
                     /locus_tag="VFD89_05280"
     CDS             2039..3700
                     /locus_tag="VFD89_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ57636.1"
                     /translation="MRKRIFYSICLACFVTLLLTATLVISVIYNNSTREVKKGVINQT
                     IFIGKAIGLDPANYVHYINSVGKEAENRISLISPDGTVIYDSFVSAEKLDNHYNRPEI
                     KSALSEGRGEATRLSDTLGEKTYYYAVKLDNGNILRVAATGKSALGIIKSASIWIVLI
                     VIAILLLAVVIARFITKIIIKPINHLDLDEPLSNITYDEFTPLLLRLEKQNQRIAMQV
                     KELTTQQEEFEYITGAMNEGLVLLGENGIVLFENNSTKRILGDAVGKSYLELYRDVNY
                     IKAVDSGLKGKPSSLKLNRDGRVYELSVNPIKGKSNAYDAVLFMVDITDREQGERMRR
                     EFTANVSHELKTPLTSIMGYAEIIEKGIAKEEDIPRFTNRIHAEAGRLLALIEDIIKL
                     SRIDEGKLYEEFEQVDLSSLCESIIDMLYEKANTRNIKISYSGETTYVYGIRSILYEM
                     IYNLCDNAISYNKDGGSINVKIDNENTRTKLSVSDTGIGIPREHHNRIFERFYRVDNS
                     HSKDTGGTGLGLAIVKHAARLHGADIALDSQEGEGTTITITFPGI"
     gene            4085..5107
                     /locus_tag="VFD89_05285"
     CDS             4085..5107
                     /locus_tag="VFD89_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZJ57637.1"
                     /translation="MRQGIAAAGNLTVDYVKTIDSYPREGNLSNILSLGKSVGGAPTN
                     VSIDLSKMDRSIPLQVIGAIGNDEDGQFLIDILKHNGIDTSQIRIDPNIGTSFTDVMS
                     VESTGSRSFFHYRGANQVLSVEHFDFSRINARILHVGYALLLDALDKQDDEYGTAMAR
                     LLSMAQSHGIKTSLDVVSENSNRFSSIIPPSLKYCNYFIVNEFESSLTTGIPDRDENG
                     RLIVENMKRICAKLLSMGVNDLVVIHAPEGGFAMEADGSYYIQPSLKLPDGYIKGAVG
                     AGDAFCAGMLYSIYKGWDTKRALEIAVSAAAACLSHINATDGMKDISAIEELYRIMEK
                     RELPMV"
     gene            5487..8309
                     /locus_tag="VFD89_05290"
     CDS             5487..8309
                     /locus_tag="VFD89_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HZJ57638.1"
                     /translation="MGIMVDKILSMQNSGNASGYWAQRILAQLGYGSKISKIKDGKYD
                     TLINNAIQHVYDKFQEESAITKDTALKAEGMILELKDDAKYFHMICCSHAHIDMNWMW
                     RYDETVAITLDTFRTMLNLMDEYPDFTFSQSQASMYKIVAEHDPAMLGEIKQRIKEGR
                     WEVTATTWVETDKNMPSGESLARHILYTKRYLSNLLDLDPHSLKLDFEPDTFGHSMNV
                     PEICSKGGIKYYYHCRGDDGECLYRWIAPSGASLIVYRELTWYNADIEPMMALYVPEF
                     CTRYGMDTMLKVYGVGDHGGGPTRRDIERIMDMNTWPVFPNIRFGRYDEYFELVEKQA
                     DNLPVVESDEQNFVFSGCYTSQSRIKMANRIAEATLNESEAFNAISHLYGAYDYSSEQ
                     FAKAWQKVLFNQFHDIIPGSGVVDTREHAMGLFQESMAIANTRRSYALRSIASRINTA
                     PLIPEDQDPSDTISEGAGVGFGIEGFRISQSERGLGKTRVFTFFNPSPYEREELSEVT
                     IWDWNYDLDRLIFKDVDGNVAEHQLIDHGFNNYWGHKYFRVLLKARAPGLGYNTYIMT
                     EKGASDISLPLPPHPRVERVDEFVLENDHIRVEFDSLTGAIVSMMDKETGAELVSPDR
                     PDGIFRFILEDESKGMTSWRVGPYMNIQELNKNVKIKRINHGPDNLRQSIGMDIEFMN
                     SKLTAIVSLDYNSPRLEFDVTCDWQEFGKPGYGIPQLNFHMPLAYKCKNYRYDIPFGT
                     IDREDRNMDLPASSWIAGMPVEEDRKGIMLITDTKYGFRGTGDALAVSLIRGSYDPDP
                     YPEVGINKFKFAVCPVYFKNNKDLIAQAYEFNHRFSVVSTSAHEGVLPAMGSFFTLKE
                     GDVAISGIKIAEDSEGNSNKLIVRVYETEGDSTKVSMGLFKEPSKAYFVDINEEKIVS
                     EGISIDGKTIELTVSPYSIANVCIEF"
     gene            8344..9360
                     /locus_tag="VFD89_05295"
     CDS             8344..9360
                     /locus_tag="VFD89_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZJ57639.1"
                     /translation="MKTSRQKVLDIFNRKNTDDKAFWTGHPNDATIPIFAREWSIEPT
                     REAIYNFLDDDCRWIVADSGYKHPEGLPAFDPAYGVDRESLSAAGCFAEAEDLKDIET
                     YPWPNPDYCDFTEVYEEVDRHQDKAIFTGMWSPFYHLIADFFGMENYFIKMYENPKVV
                     EAATERIVDFYVAANDKFFAGLGDRGDTMFFGNDFGTQLDLFISPDNFRRFVLPSFKR
                     LIEVGKKHNKKIMLHSCGSIYRIIPDLIDAGVDVLHPIQAQARGMSAKELAQYKNDLA
                     FVGGIDAQNFIVNATPEEVREEVKRVRDILGPNIVISPSHEEILPNVPSANTLAMARA
                     AKED"
     gene            9567..9992
                     /locus_tag="VFD89_05300"
     CDS             9567..9992
                     /locus_tag="VFD89_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZJ57640.1"
                     /translation="MENELKIKMYSFTLDCKEPHRLAEFYGSLMGWEIMALGDDWACV
                     YAPGTNQGGYPCILFQSNPDYEPPVWPEETEAQQQMAHIDFAVNDLEKAVQHAVQCGA
                     AIADEQFSEDWTVMLDPAGHPFCLCDMKSIIESPHFGLL"
     gene            complement(10101..10628)
                     /locus_tag="VFD89_05305"
     CDS             complement(10101..10628)
                     /locus_tag="VFD89_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZJ57641.1"
                     /translation="MSFLELAKKRYSVRSFRDAPVEKDKIVKVLEAGRLSPSACNFQP
                     RYFVVIEDEDLKEKIGQCYPRQWFKQAPVIIAVCGDHSASWKRGDGKDHCDIDVAIAV
                     DHMTLAAADQGLGTCWICAFDAKLCHEMLELPNHMEVVALLPLGYPEKEGDSERHDKA
                     RKPLDEMVGWNRYQK"
     gene            11026..12528
                     /locus_tag="VFD89_05310"
     CDS             11026..12528
                     /locus_tag="VFD89_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57642.1"
                     /translation="MLSFKNLTPTNGFDSGNLNNARQNNYAWSMSDLGDYIYVGTGRN
                     IPLNIIAAIVPGVQLPALISANPIDNLAEIWRYRKDGSLPWERVYKAPANSGIIGFRY
                     MITHRPFGGNPCLYAATVGPSPQVLKSTNGVNWFVLPDSVLQGNSSRAMVVQKGKIYM
                     STISEQDETAGALLYSSPDPEFYPWESLIDPDAPGFDPSKNPTGPIWNMEVFNNRIYV
                     TVSDPNGAQVWRTNGPEPKLNDWTLIVDRGFGNPANVITLATGVFKDHLYVSATKELP
                     LSWLIPRGCDIIRINKRDEWELVVGGNPFLPASNSASGLGSGFNNPFNVYAWQIREHK
                     GKLLITTFDDSSNMQLILDTLLANRTALEERIGSTVTNILIGIYRSIVEILNAIRYPI
                     GFDLYTSTDGIHFSKVFLNGLNNRNNYGGRILYEDSSKCLYLGTANPFEGCEVWKVNP
                     KDWNPKPCNDDHYKGLREIRKIMVKNFRVMNENMPAVLDFIPKHFTQKFC"
     gene            13077..15011
                     /locus_tag="VFD89_05315"
     CDS             13077..15011
                     /locus_tag="VFD89_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZJ57643.1"
                     /translation="MDYKCLFTPFKIGKMEVKNRIVMSPMGLNAAHPDGRIDDDEIHY
                     FEERARGGVGLIIMGCQFITEELAQGSMEGYLDKTHVIPPLTNLCEAVQKYGTKISAQ
                     ISCGTGKNAFSNMLGDVPISASEIPSMFDPNLLCRALTIEEIDEIMEQFAFSAKLLKD
                     AGFDAIEVHAHAGYLVDQFMSAVWNKRTDKYGGSLENRMRFPVEIVQAIRGAVGPDYP
                     ILFRIAVKHHFEGGRTIEETLPMLKILQDAGVDALDIDSGSYENIEYIFPPAYLGDAC
                     MADVCAPARQAVDIPLLNSGNHTPETAVELIESGNADFVMFGRPLIADPYIPHKLLNE
                     KRDEVRPCIRCNDDCIGRIITRLTKISCSVNPVAGFENRMDENYGEKGKKVVVVGGGP
                     GGMEAARSAAKAGHEVILFEKDGELGGQLKSAATPDFKSQLRELVEWYKRQLEIHKVD
                     VRLNTGANEDLSELEEADAIIVATGAVPLMPDIKGIENAVDIISAHTNKEKLKGERII
                     YCGGGLSACDSALENAMEGKKVTIVEMLDDVAVDDHFINKAALIPMLNKHGVEIYTGH
                     RVTEITPDGVKAVTKDGEEVFIEGDTMVAAFGMKPNNCVSEKIYSEYHIKTRIIGDCQ
                     KVGRVGNAIREGFYAGRYIG"
     gene            15305..16105
                     /locus_tag="VFD89_05320"
     CDS             15305..16105
                     /locus_tag="VFD89_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZJ57644.1"
                     /translation="MIVLNKKLEIKELKPYLYLLDEAHESTGYLLIGEDKACLIDTMM
                     GYNDLYQAVRSITDKPLIVINTHGHPDHIYGNVYFDKAYMNPADREVAKIFTESPDFV
                     AECKKRDLQMPPFEDIHEGDVIDLGGRTLKVYQLPGHTPGGILLLFPEDRILFTGDTI
                     NHHVWMQLPGCLSLEQFAQELERLMFLEGEADFILHGHARDYDDISLMSAVLNGVREI
                     IAGKTEADKPYRWFGGTDLQHPFEVDPDAHFQQDNHVICYREDNIRRK"
BASE COUNT         4930 a         3153 c         3861 g         4247 t
ORIGIN      
        1 atattatggg gattttcagg cacttaaaaa tattaatatg agcatcccgg ccaacgaaat
       61 caccgccttt attgggccat cgggctgcgg taaatcaacc ttgttaaaaa gcctcaaccg
      121 gatgaacgat cttattgagg ggtgcaggat tgacggtgaa gtgtttttgg atgatacaaa
      181 tatatatgaa aatattgatg tcaatctgtt gcgcaagcgt gtcggcatgg tatttcaaaa
      241 gccaaaccct tttcctatga gtgtatatga taatgttgca tatgggccga gaacacatgg
      301 tattaaatca aaggccaagc tggatgatat tgttgagcgt tccctgaccg atgccgctat
      361 ctggaatgat cttaaggaca ggcttaagtc aagcgctctt agcctttcgg gcggacaaca
      421 gcaaaggctc tgtatagcca gggcattggc cgtagagcct gaagtgcttt taatggacga
      481 acctacaagt gcattggacc ccatctcaac gagcaagata gaggaccttg cactggaact
      541 taaaaagaaa tatactatag tcatggttac tcataatatg cagcaggcca cccgtatttc
      601 agacaaaaca gcattttttc tattaggaga gataatagag atggatgaaa cggagaaatt
      661 gtttgcaaat ccaggtgaca agcgaacaga ggattatatt acggggaggt ttggataatg
      721 agaagcagat tcgatgccca actcgatatt ctaaacaaca gcctgatctc tatgggcgca
      781 ctttgtgaga atgctatcgc cagcgctgtt aaggcattgt tgcataatga tatgtccctg
      841 agcaaaaagg ccatagaaac agaggtagag cttgatcaga tggaaaagga tattgaaagg
      901 ctctgcctaa ggctacttct gcagcatcat ccggttgcag gtgacttgcg ccttatttcc
      961 gccgctctga agatgattac ggatatggaa cgcatcggcg accaggctgc ggatattgca
     1021 gagattgttg catatgttga tctgaccgac aatataaaca aggtgcggat cgccgatatg
     1081 gcaaaggcca ccataaaaat ggttaccgag agtatagacg cctttgtcaa gcgcgattta
     1141 aaagtggcgg aagatgtcat cgactacgat gatgtagtgg atgatctatt caataagatt
     1201 aaagatgatg ctattgcact cataactgag aatccggaat acggtgagca attcgttgat
     1261 atagtcatga tagctaaata ccttgaaaga atcggggatc acgcaaccaa tattgcagaa
     1321 tgggtagtat tttccataac aggtgagcac aaggagcaat aacatgatct attatgttga
     1381 ggatgacgag ggtatccgcg aactggtagt ttataccttg cagaatacgg gttttcaggc
     1441 tgtgggattt gagaatggtc aacagctgtt cagtgccctt caaaacacaa aacctcaact
     1501 gattttgctt gatataatgc tgcccggtga cgatggcatg acaattctca ggcgtttgcg
     1561 ttcagacatt gcaacaagga agatacccgt tatcatgata acagcaaaag gatccgagta
     1621 cgacaaggtc attggattgg attcgggagc agacgactat gtaacaaaac cctttggtat
     1681 gatggaactg gtttcgcgga taagggcagt gctccgccgt actggagttg tagagaacca
     1741 tgaagtgtac agctataagt ctgttgttct tgacactcgt aaacacagcg tgaccgtaaa
     1801 ggatagggtt gttgacttaa cacttaagga atttgaacta ttaaagcttt taatgaaaaa
     1861 tgcgggagct gttctaacca gggacatgtt gctggagaat atctggggat atgattttga
     1921 cggtgaaacc agaactgtcg acgtccatat acgaaccttg agacaaaaac tgagtgatga
     1981 aggtaaaata atcgagactg tgcgcggtgt cggatataga ttagggggca cagagtaaat
     2041 gagaaagcga atattttaca gcatttgcct cgcctgcttt gttactcttc tattgacggc
     2101 aacacttgtt atcagcgtaa tttacaataa ctcaaccagg gaagtaaaaa agggagtaat
     2161 taaccaaacc atctttattg gcaaggccat tgggttggat cctgctaact atgttcatta
     2221 tatcaacagt gttggtaagg aggccgaaaa ccgcatatcg ctgatttctc cggatgggac
     2281 ggtaatatac gatagcttcg tatcagcgga aaagctggat aaccactata acaggcctga
     2341 aataaaatcc gccctatcgg agggcagggg agaggccacc cggctttccg atactctggg
     2401 tgaaaaaacc tactactatg ctgtaaagct ggacaatggg aacatactaa gggttgctgc
     2461 aaccggtaaa agtgcccttg gtattataaa aagtgcatct atttggatag tgttaatagt
     2521 tattgccatt cttcttttag cagtagtcat tgccagattc attaccaaaa ttattatcaa
     2581 gccaataaac catttagacc tggatgaacc cctctccaat atcacctacg atgaattcac
     2641 acctcttttg ttgagattgg aaaaacaaaa ccaaaggatt gcaatgcagg taaaggagtt
     2701 aacaacgcag caggaagaat ttgaatacat aaccggggcc atgaacgaag gacttgtttt
     2761 gctgggtgaa aatggcattg tcctcttcga aaacaacagt accaagcgca tcctgggtga
     2821 tgctgtggga aaatcatact tagagctata ccgagatgta aattacataa aggctgttga
     2881 ttcaggactg aaaggtaaac cctcaagctt aaagttgaac agggacggaa gggtttatga
     2941 gctgtccgta aaccccatca agggcaaatc caacgcatat gatgctgttt tatttatggt
     3001 tgatatcacg gatagggagc aaggagagag gatgcgccgt gagtttacgg ctaatgtttc
     3061 acacgaactt aaaacacccc tgacctctat catgggctac gccgaaataa ttgaaaaagg
     3121 tatagccaag gaggaggata taccacgctt taccaaccgt attcacgccg aggctggccg
     3181 cttactggct ttgatagagg atattatcaa attatcaaga atagatgaag gtaaattata
     3241 tgaggagttt gagcaggtag acctatccag tctatgtgaa tccattatag atatgctata
     3301 tgagaaagca aatacaagaa acataaaaat aagctattct ggtgaaacca cttacgtata
     3361 cggcataagg tcaatactct atgagatgat ctataatctc tgcgacaacg ccatctccta
     3421 taacaaggat gggggcagca ttaacgttaa gatagataat gaaaacacca ggacaaaatt
     3481 atctgtttcc gatacaggca taggtattcc aagagaacac cataaccgta tatttgaacg
     3541 tttttaccgg gttgataata gccattccaa ggataccggg ggcactggtc tgggcctggc
     3601 aatcgttaag catgccgcca ggctccatgg agctgatatc gcccttgaca gccaggaagg
     3661 cgaggggacg accataacaa ttacctttcc aggcatttag tcggcagccg cctatctttt
     3721 gtgtagtaac ttcagtttat gaccggtctt attaagtttt tcatgattaa ggtagtttca
     3781 taattattag gaaaatgtgt tgtgccattc aatcataggc cggtaagggc tattgaccgc
     3841 ttatcaatag ctcattacca gggcaaattt agctatatgg caggatttgc cgaatctatg
     3901 gcgaataaaa gtagatgagg atggattaca agtacaaaag aaattcgtct taagtattta
     3961 tccaatataa attttcagac taaagggcct taaatcaagg tttattttca cgtttaaaat
     4021 cgtatgataa tttgactact gtcatcatcc aggaatacaa cagcatgtaa aggagtaacc
     4081 agatatgagg caaggaatag cagcagcagg caacctaact gtagactatg taaagaccat
     4141 agacagttac cccagggaag gcaacctctc aaacattctc tcattgggaa aatcagttgg
     4201 aggtgctccg actaacgttt ccattgattt atccaaaatg gacagatcca ttcctctcca
     4261 agtcatcgga gcaatcggga atgatgagga cggacaattt ttaattgata ttttaaagca
     4321 taatgggata gataccagtc agataagaat agaccccaat attggtactt ccttcaccga
     4381 tgtaatgtcc gtcgaatcca caggatctcg ttcatttttt cattataggg gtgcaaatca
     4441 ggtattaagt gttgagcatt ttgatttcag caggataaat gccaggatcc tccatgttgg
     4501 gtatgcatta ctgctggatg ccctcgataa acaggacgat gagtatggca ccgcaatggc
     4561 caggctctta tccatggccc aaagtcatgg catcaagaca tctttggatg ttgtaagtga
     4621 gaacagtaac agattttcaa gtataatacc ccccagctta aaatactgta attatttcat
     4681 tgtcaatgaa tttgaatcat cgctaaccac gggcattccc gatagggatg aaaatggtcg
     4741 gctaatcgtc gaaaacatga agcggatatg tgcaaaactc ttatctatgg gagtcaatga
     4801 tctggtggtc atccatgctc ctgaaggagg atttgctatg gaggcagacg gcagctacta
     4861 catacagccc tcccttaagt tgccggatgg ctatattaag ggtgcggtag gggccggtga
     4921 tgccttctgc gcagggatgc tatatagcat ctataaaggt tgggatacaa aaagggctct
     4981 ggagatagct gtgagtgcag cagctgcctg tctgtcccat atcaatgcta ctgatgggat
     5041 gaaggatata tccgccatcg aagagttgta caggataatg gagaagagag agttacccat
     5101 ggtataggta atgataattt aatgccttat gcttgttaga tatatcggta atggttatta
     5161 aacgataagt tatatctttg tattggagga tggtttattc cggctgcttc caaaccccac
     5221 aaatccatcc tcctcagtaa cagaactaat acaccgaccg acattgcata tagctaacag
     5281 taaattgttt gttatggcaa tgagctatta aaaagcgaat gacttgagga gcattcaata
     5341 gctatcaata gcacattaac cttcttaacc agtaagctat tgaagctaat ttgattaaat
     5401 ccagatgcag ggagattctt cgagtaattt gaggatgaac ctgcactggt ataataagca
     5461 agcaataaat aagggggaat aattgcatgg gtattatggt ggataagatc ttgtctatgc
     5521 aaaacagtgg gaatgcttct ggctattggg ctcaaagaat tctggcacag ctggggtacg
     5581 gctcaaaaat atcaaagata aaggatggaa agtacgatac cctaatcaac aatgcaattc
     5641 aacatgttta tgacaaattc caggaagaga gcgccataac aaaggacact gcattaaagg
     5701 cagaagggat gatattggag ctaaaggatg atgcgaaata ttttcatatg atatgctgct
     5761 cccatgccca tatcgatatg aattggatgt ggcgctacga tgagacggtg gctataacgt
     5821 tggatacctt tcgcaccatg ctcaacttaa tggatgagta ccctgacttt actttttctc
     5881 aatctcaagc ttccatgtac aaaattgttg cagaacatga tccggctatg ctgggagaga
     5941 taaagcaaag gattaaggaa ggaagatggg aggtaaccgc taccacctgg gtagagaccg
     6001 acaagaatat gccaagcgga gagagtttgg ccaggcatat cctctatacc aaaagatatc
     6061 tgtcaaatct cctggacctg gatccacatt cactcaagct tgactttgaa cctgatacct
     6121 tcgggcatag tatgaatgta cccgagatat gctcaaaggg tggaataaag tattattacc
     6181 actgcagggg ggatgatggg gagtgtttat atagatggat agccccatca ggtgcatccc
     6241 taatcgtgta cagggaactg acctggtata atgctgatat tgagccgatg atggcactgt
     6301 atgtcccgga attttgcacc aggtatggca tggataccat gctgaaggtc tatggtgtag
     6361 gcgatcatgg cggtggtcct accaggcggg atatagagag aataatggat atgaacacct
     6421 ggcctgtatt ccccaatata aggtttggca ggtacgatga atactttgag cttgttgaaa
     6481 aacaggcgga caacctgcct gtagttgaaa gcgatgagca aaactttgtc ttttcaggct
     6541 gctatacatc ccagagcaga ataaagatgg ctaacaggat agcggaagct accctgaatg
     6601 aatctgaagc ctttaatgca atctcccatc tatacggggc ctatgactat tcttcggagc
     6661 aattcgccaa agcatggcag aaggtacttt ttaaccaatt tcatgatata attccgggtt
     6721 ccggagtagt cgataccagg gaacatgcca tgggtctctt ccaggagagc atggcaatag
     6781 caaatacacg aagaagctat gccttaagaa gtattgcatc caggataaat acagccccac
     6841 taataccgga agaccaagat cccagcgata caatatcgga aggcgcgggg gtaggttttg
     6901 gtatagaagg gttcaggatc agtcaatcag aaaggggact gggtaaaacc agagtcttca
     6961 ccttctttaa cccctctccc tatgaacggg aagagcttag cgaagttaca atctgggatt
     7021 ggaattatga tcttgacaga ttgatcttca aggacgttga tggcaatgta gcggagcatc
     7081 agctaataga tcatgggttt aataactatt ggggtcataa atattttaga gtattgctta
     7141 aagcccgtgc tcccggctta ggatacaata cctatattat gacagaaaag ggagcatccg
     7201 atataagtct tcccttgcct ccacatccaa gggtagaaag ggtagatgag tttgtcctgg
     7261 agaatgatca tatcagagtc gaatttgatt ccttaaccgg ggctatagtg tcaatgatgg
     7321 ataaagaaac gggtgcggag ttggtgtccc ccgatcgacc ggatggtatc tttaggttca
     7381 ttctggagga tgaaagcaag ggcatgacat cctggagagt gggcccttat atgaatattc
     7441 aggagctcaa caaaaacgta aagataaaaa ggataaatca cggacctgat aacttaagac
     7501 agtccatagg catggatatt gagtttatga actctaaact caccgctata gtatctctgg
     7561 actacaacag ccccagattg gaatttgacg taacctgcga ttggcaggaa tttggtaagc
     7621 caggttatgg aataccccag ctgaatttcc atatgcccct ggcatacaag tgtaagaact
     7681 acaggtatga catccccttt ggaaccattg acagggaaga taggaatatg gatctgcctg
     7741 ctagcagctg gatagcggga atgcccgttg aagaggacag gaagggtata atgctgatta
     7801 cggataccaa atatgggttt aggggaaccg gtgatgcatt ggctgtaagt cttataaggg
     7861 gatcctacga tcctgatcct tatccggagg ttggcatcaa taagtttaag tttgcagtat
     7921 gccctgtcta ctttaagaac aataaggatt taatagccca ggcttatgaa tttaaccata
     7981 gattcagcgt ggtatccaca tctgcccatg aaggagttct gcctgcaatg ggaagcttct
     8041 ttaccttaaa ggaaggggat gtggccatat cgggaataaa aatagcagaa gactccgagg
     8101 gcaatagcaa caagcttata gtccgggtct atgaaacaga gggagacagt accaaggtat
     8161 ctatgggctt gtttaaagaa ccatcaaagg catactttgt agatataaat gaggagaaga
     8221 ttgtctccga aggcatcagc atagatggta aaaccataga attaaccgta tctccctaca
     8281 gcatcgctaa tgtatgtatt gagttttaag ccgtaactac tattttacaa tggagtaagc
     8341 gtgatgaaga catcaagaca gaaggtattg gatattttca atcgtaagaa tacggatgat
     8401 aaggcatttt ggaccggtca tcccaacgat gccaccattc ccatttttgc cagggaatgg
     8461 agtatcgaac cgacccgtga ggccatttac aactttctcg atgatgactg caggtggata
     8521 gtggccgaca gcggctacaa acatccggag ggtttgcctg catttgatcc agcctatggt
     8581 gtggacaggg aatccctaag tgcagcgggg tgctttgcag aggcagagga ccttaaggac
     8641 atagagacat acccctggcc caacccggat tactgcgact ttaccgaagt atacgaagag
     8701 gtcgaccggc atcaggataa ggccatattt acgggaatgt ggagtccatt ttaccatctg
     8761 attgcagact tttttggcat ggaaaactat ttcattaaga tgtacgaaaa ccctaaggta
     8821 gttgaggcgg ccacggagag gattgtagat ttctatgtgg ctgccaacga caagttcttc
     8881 gcaggccttg gagaccgagg ggataccatg ttctttggaa atgattttgg tacccaactg
     8941 gatctgttta tttctccgga taattttcga aggtttgtgt tgccctcctt taagaggctg
     9001 attgaagtgg ggaagaaaca caataaaaag atcatgctcc attcctgtgg gtccatctac
     9061 aggataatac cggatttgat cgatgccggt gtggatgtcc ttcatcccat acaggcccag
     9121 gccaggggga tgtcagcaaa ggagttggcg caatacaaga atgatctggc ttttgtaggt
     9181 ggcattgatg cccaaaattt tattgtaaat gctacgccgg aggaagtcag ggaggaagtt
     9241 aagcgggtaa gggatattct ggggcccaat atagttatat ccccaagcca tgaggagata
     9301 ctgccgaatg tcccttctgc aaataccctt gcaatggcca gggcagccaa agaggattag
     9361 acccaatcct gcatcctacc gaaggtagat gctttgaaat tccacttatt tatttcgtat
     9421 gggaggatgg tttattctgg cagattccaa attaatccac aaatccatat caatgaagtc
     9481 atccgcttat caatagtcca ttacctggtc aataaactgg gacaagatgt aagctataga
     9541 tctaataata gggaggatat agggatatgg aaaatgaact aaaaattaaa atgtactcat
     9601 tcacgctgga ttgcaaagaa ccccacaggt tagcagagtt ttatgggtcc ctgatggggt
     9661 gggagataat ggccttaggt gatgattggg cgtgcgtata cgcaccgggc accaatcagg
     9721 gggggtatcc ctgcatcctg tttcaaagca accccgacta tgaaccgcct gtatggccgg
     9781 aagaaactga agcacaacag cagatggccc atatcgattt tgccgtcaac gatttggaaa
     9841 aggccgttca acatgcagtc cagtgcggag cagccattgc cgatgaacag ttctccgaag
     9901 attggacggt tatgcttgac cccgccggtc atcccttctg cctgtgtgat atgaaatcca
     9961 tcattgagag tcctcatttc ggcctgttat aaggaatcag gatactgatg gaagatttac
    10021 gggatcgata ataatttttc ctggtatcaa gaacagtata gggctatttg tttagcccta
    10081 tacttattta taattacgat tcacttttgg tatctgttcc aaccaaccat ttcatccagg
    10141 ggcttgcgtg ctttgtcgtg gcgttcacta tcgccttctt tttcgggata acccaaaggc
    10201 aacagggcta ctacttccat atggttaggt aattccagca tttcatgaca aagctttgcg
    10261 tcaaaggcac atatccagca ggtgcctaaa ccttggtctg ctgcagccag agtcatatgg
    10321 tcaactgcaa tggccacgtc aatatcacag tgatccttgc cgtctcctct cttccaggaa
    10381 gcactgtgat cgccgcatac agcaataatg accggggctt gtttaaacca ctgcctggga
    10441 tagcattgtc caatcttctc ctttaaatct tcatcctcaa taactacaaa gtagcggggc
    10501 tgaaaattac aggcagatgg agacagcctt cctgcttcca gaaccttaac aatcttatcc
    10561 ttctccactg gggcatccct gaaacttctt accgagtatc ttttctttgc cagttccaaa
    10621 aaactcatat atactacctc caatcatatt cttaaatcgg tacaatccgc tgggtatgaa
    10681 aagcatcata agatgctggt aaagagactt attattccca ctatcaccta taatgaaatt
    10741 attccgtatt atgtatttca tgtttttgct gtttggaagg aagggtatat ttttcctgtt
    10801 atcctggatt acaattgctt acagtgatct attattactt taattatact ccttaatcgc
    10861 atctccggca aatccatata ggttaacata tcccaaagta gatgcatagt tatatagata
    10921 tccattgcaa aacagtcaca tacctccttt tggaacatat actgtaaatg caaggtatat
    10981 agaaagccat atcttgcaat atatctaaac aggaggcaaa taagtatgtt gagttttaag
    11041 aatttaactc cgacaaatgg atttgactct ggcaatctaa acaatgcaag gcagaacaat
    11101 tacgcatggt ctatgagtga tttgggagat tatatatatg taggaaccgg taggaatata
    11161 ccgcttaata ttattgccgc tatcgtgccg ggagtgcaac ttcctgcact gataagcgca
    11221 aatccaatag ataatctggc agaaatctgg aggtatagaa aggatgggtc tcttccctgg
    11281 gaaagggtct ataaagcccc agccaattcc ggtattatag gttttaggta tatgataacc
    11341 cataggcctt ttggaggaaa tccttgcctc tatgccgcaa cggtaggccc aagtccacag
    11401 gtactaaaga gcaccaatgg tgtcaattgg tttgtcttac ccgattcagt cctgcaggga
    11461 aattcatcaa gagccatggt agtacagaag ggcaaaattt atatgtctac catatcagag
    11521 caggatgaga ccgccggagc attgctgtac agcagtccgg atccggagtt ctatccctgg
    11581 gaaagcctta tagatccgga tgcacccggt tttgatccta gcaagaaccc gacaggcccc
    11641 atatggaata tggaagtctt taataacagg atctatgtta ccgtaagcga ccccaatggt
    11701 gcccaggttt ggagaacaaa cggtcctgaa ccaaaactaa atgactggac cctcatagtt
    11761 gacaggggat ttggaaaccc tgcaaatgtt attaccctgg ccacgggtgt atttaaagat
    11821 catctttatg taagtgcaac caaggagtta cccttgtcct ggctcatccc caggggctgc
    11881 gatatcatca ggataaacaa gcgtgatgaa tgggagctgg ttgtaggagg aaatccgttt
    11941 ttgcctgcat caaatagtgc ttccggtctt gggtcaggct tcaataatcc tttcaatgtc
    12001 tacgcatggc agatacggga acacaagggc aagcttctaa taaccacttt cgatgattcc
    12061 agcaatatgc agttgatcct ggacaccctt ttggctaaca ggacggcatt ggaggagcgg
    12121 atagggtcaa cggtaacaaa tatattgatt ggtatttaca ggagtatcgt tgaaatatta
    12181 aacgcaataa gatatccgat aggatttgat ctatacacgt ctacggacgg cattcatttt
    12241 agcaaagttt tcctaaatgg actgaacaac aggaataatt atggtggcag gatattatac
    12301 gaggactcct caaagtgttt gtatttggga acagccaacc cgtttgaagg atgtgaagtc
    12361 tggaaggtga atcccaagga ctggaaccca aaaccctgta atgacgatca ttataagggg
    12421 ttgagggaga tcagaaagat aatggttaaa aactttagag ttatgaatga gaatatgccg
    12481 gccgttcttg actttatccc caagcatttt acccagaagt tttgctgaac aatagccatt
    12541 accggcatat gattgactag cataatgcgt taagttagat aaaatatata gatcttttat
    12601 agggctccat ttatcccatg aataagagta gagtaggaaa ggggtaatag gcgccctctt
    12661 gttttcccca tattattaac aacagttaag aagggacagg aattcaattg acaataataa
    12721 attgaaaaaa ttcatgtact cagttttagc caacgacagg aaacgataat aacttatatt
    12781 atttcctaaa atgcaagaaa attaggaata aaaacaatat tattgacaaa ggatagtttt
    12841 gccatatatc ataaatataa ctagattcag gggggtgata atgcaatcct gactattggc
    12901 ttcctatctt cgcagttgaa gggataatct gagtttcaga gtacctcagg cagtagaacc
    12961 gaattatcgg gtttggctaa aggtggtatc agtttgaaca tttaggtgct tacataggta
    13021 tctaaaagca aaataaggaa tactactaag gtgaatttta ttaaggaggt agaaaaatgg
    13081 attacaaatg tttgtttact ccctttaaaa tagggaagat ggaagtaaag aacaggatag
    13141 taatgtcacc catgggctta aatgctgccc atcccgatgg gagaattgac gatgatgaaa
    13201 ttcattattt tgaagaacgg gcccgagggg gagtagggct catcattatg ggttgccagt
    13261 tcattacgga agagcttgcc caaggctcca tggaaggcta tttggataag acccatgtaa
    13321 tacccccgct aaccaaccta tgcgaagcag ttcagaaata tggcacgaaa atcagcgccc
    13381 aaataagctg tggaaccggg aaaaacgcct ttagcaacat gttgggcgat gttcccatat
    13441 cagcgtcgga aataccctcc atgtttgatc caaatctttt gtgtcgtgcc cttaccattg
    13501 aagaaataga tgagataatg gagcagtttg ccttttcagc gaaattatta aaggatgcag
    13561 gctttgacgc tatagaggta catgcacatg ccggttacct ggttgatcag tttatgtctg
    13621 ccgtctggaa caagagaact gataaatacg gtggttccct ggagaacagg atgagattcc
    13681 ccgttgaaat cgttcaggca atcagggggg ctgttggacc tgattatccc atcctgttca
    13741 gaattgccgt aaagcatcac tttgaaggcg gacgtaccat tgaggagacc ctgcccatgc
    13801 ttaaaatact ccaggatgcc ggggttgatg cccttgacat cgacagcgga agctatgaga
    13861 acatagaata catatttcca cctgcatacc tgggagatgc atgtatggcg gatgtatgtg
    13921 ctccggcaag acaagcagtg gatatcccgc tgctaaactc gggtaaccat acccctgaga
    13981 ctgccgtaga gttaatagaa tcggggaacg ctgactttgt aatgtttgga cgtcctctaa
    14041 tcgccgatcc ctatattcca cataagctgc ttaatgaaaa gagggatgaa gtacgtccat
    14101 gtatacggtg caacgatgac tgcataggga gaattattac ccggctgaca aagataagct
    14161 gttctgttaa ccctgtagca ggatttgaaa accgtatgga cgagaactat ggagaaaaag
    14221 ggaagaaggt agttgtagtc gggggagggc ccgggggaat ggaggcggca cgaagtgcag
    14281 caaaggccgg acatgaggta atactctttg aaaaggatgg agagttggga ggccagctca
    14341 agagcgctgc aacccctgat ttcaagtctc agttacgtga attggtagaa tggtacaaac
    14401 ggcagctgga gatacacaag gtggatgtcc gtcttaatac tggtgcaaat gaggatttgt
    14461 ccgaactgga agaggccgat gcgattattg tggctaccgg tgcagtgcct ctgatgccgg
    14521 acattaaagg tatagagaat gcagttgata taataagtgc ccatacaaac aaggaaaagc
    14581 taaagggaga aagaataata tactgcggcg gaggactttc tgcttgtgac agtgccctgg
    14641 aaaatgccat ggaaggcaaa aaggtgacca ttgttgagat gctggacgac gttgcagtag
    14701 atgaccactt cattaacaaa gcagccctta ttcctatgct gaacaagcat ggggtggaga
    14761 tctataccgg gcatagggtt acagaaataa cccctgatgg tgtcaaggct gtaacaaagg
    14821 acggcgagga agtgtttatc gaaggggata ccatggttgc cgcctttggt atgaagccca
    14881 ataattgtgt cagtgaaaaa atatactcag aatatcacat taagacccgt atcatagggg
    14941 actgccagaa agtaggcagg gtaggaaatg ctatccgtga aggcttctat gcgggtaggt
    15001 atataggata actttaatca atcatttgct ggtaatgact attgtccgca acttctaaat
    15061 gaagtcaccc gtttatcaat agctcattac ctaagattat gaactagcta ccagaggtta
    15121 agtagagtat aggattaatc aggcattgca cttaagttta ggtgcaatgc ctgtcctatg
    15181 tttttaaaaa caattgtgat agaatagatc catagaaaag gagaaattaa gccaggacag
    15241 aaggggttgc agacctgaaa ccttgcttaa cagtctagcc ttaacagtaa atcatgtaga
    15301 tggggtgata gtattgaata aaaagttgga aattaaggag cttaaaccat atctctacct
    15361 cctggatgaa gctcatgaat caacaggata tctgctcata ggcgaggata aggcctgtct
    15421 tatcgatacc atgatgggct acaatgacct ctatcaggct gtcaggtcta taaccgacaa
    15481 gccgctgatt gtcataaaca cccatggaca cccggatcat atttatggga atgtgtattt
    15541 tgataaggca tatatgaatc ctgcggatcg tgaagtggcc aaaatattta ctgaaagtcc
    15601 ggatttcgtt gcagagtgta aaaaaagaga tctccagatg cctccctttg aagatatcca
    15661 tgaaggggat gtgatagatc ttggaggacg aacccttaag gtctatcaac ttccaggaca
    15721 caccccgggc ggaatacttc ttcttttccc agaagatcgt attctattta ctggtgatac
    15781 tattaaccac catgtatgga tgcagctacc gggatgttta agtctggaac aatttgccca
    15841 ggaactggag aggctgatgt ttttggaagg ggaggctgac tttatccttc acggtcatgc
    15901 cagggattat gacgacatat cccttatgag tgcggtcctt aacggtgtca gggaaataat
    15961 agccggcaag acggaagccg ataaacctta ccgatggttc gggggcacgg atttacaaca
    16021 tccatttgag gttgatcccg atgcacattt ccaacaggat aatcatgtga tttgctacag
    16081 ggaagataat ataagacgaa aatagtacga ccatttgcta tcatttatat agacaattta
    16141 tcgggtttat gtaaacccta tcagttgcca gggagaatat ctgccgtata t
//
LOCUS       DASRNX010000131         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_608690,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000131 DASRNX010000000
VERSION     DASRNX010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608690"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(454..738)
                     /locus_tag="VFD89_05325"
     CDS             complement(454..738)
                     /locus_tag="VFD89_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57645.1"
                     /translation="MEFFEDLGKKVGETVKVIGDRSHQVLEIGRINIEIGKEEASIKR
                     LYTQIGEAVYRAHTKGDETAEVIDNLCQEIAERWNRVKEFKIRISELKSP"
     gene            complement(816..1922)
                     /locus_tag="VFD89_05330"
     CDS             complement(816..1922)
                     /locus_tag="VFD89_05330"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HZJ57646.1"
                     /translation="MDKDLLKQRLIEYGMDLGFGLIGFTDAKPLELWDSQISGRGEDD
                     PESIHLWDNMEHDPKRIMPTAKSVIVAAWPYTPYKANFPEGIGRFSAHYREYPKGRDV
                     AFELGRFLKKVGYEAMVHPPLPAKELALRAGIGYFGKNSLIHTEKYGSWISLHFILTD
                     ANLSPDTGMETLTDCGDCDLCISACPNGAIKTDGRVIPSKCLRHHMLSSDFVPGDIRD
                     KIGNKMLGCDICQMVCPFNKCGVSAASLPAYDEMTLFDIGNILNEWPAGLKKRMDRMG
                     QLIGQNYARSQKVLSAAIIFAGNSKDRSYLPDLKRLLKHSHGPIRGHSAWAIGEIGSL
                     GEGEYYKEILEKAIRDEKDARVIEEMSDAIDRLG"
     gene            complement(1953..>2662)
                     /gene="trpS"
                     /locus_tag="VFD89_05335"
     CDS             complement(1953..>2662)
                     /gene="trpS"
                     /locus_tag="VFD89_05335"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZJ57647.1"
                     /translation="FTYTGELGRMTQFKEKSQRHADNINAGLFTYPVLMAADILLYQA
                     DLVPVGEDQKQHLEITRDIAQRFNNIYGDVFRIPEPYIPKVGARIMSLQEPEKKMSKS
                     DDNPNSYISLLDEPDVIIRKFRRAVTDSEAMVRYDEKIKPGISNLMSIYSSITEKSYG
                     TIEKDFEGLGYGDFKAAVGQVVAETLRPIQEKHDELIADKAYLDNIMKDGAQRAESLS
                     YKTLRKVYKKVGLVPRG"
BASE COUNT          652 a          709 c          530 g          771 t
ORIGIN      
        1 cggaagcaat tgcattggca attgcaacac acaggtttca acgaggcggg tgatttgtac
       61 ccaccacctg ttttactaat cggaattcgc tgcttataga agcgggagac atcttaccga
      121 ctcgttataa catcggaagc aattgcattg gcaattgcaa cacacaggtt tcaacgaggc
      181 gggtgatttg tacccaccac ctgttttact aatcggaatt cgctgcttat agaagcggga
      241 gacattctgc cgcctcgtta tagaaaaacc cgctattttg ataaaggcaa aatcatccgc
      301 ttgcatatcg attaatatct tttccatgat gggtaagccc aacaaaatga ttcgagcttt
      361 tcaaatgcca atgtaggttg tcccccgtaa atcgcttaat acgggctccg atagaccctt
      421 atgacattgg actcaaagcc ggcacaatag ggcttaaggg gactttagct cactgatccg
      481 aatcttaaat tccttcactc tattccatcg ttccgcaatt tcctgacata aattgtcgat
      541 gacttcagcc gtctcgtcac ccttagtatg cgccctatat acagcctctc caatttgggt
      601 atagagtctc tttatgctcg cctcttcttt cccgatttca atatttattc ttcctatctc
      661 aagtacctga tgggatctat cgccgattac ctttacggtt tcgcccacct ttttccctag
      721 atcttcaaag aattccatta tagcccgctc ccttctccct ctattcttcc ccttttacac
      781 cggaaaaaac aaaataatcg taaggattat gggtattaac ctaatcgatc aatcgcatct
      841 gacatctcct ctatgaccct tgcatctttc tcatccctta tggctttctc caggatctcc
      901 ttataatatt cgccctcgcc caaagatcca atttcaccaa tggcccaagc gctgtgtccc
      961 cttatggggc cgtgggaatg cttcagcaac cgctttaggt cgggtaggta ggatctatcc
     1021 ttactgttgc ccgcaaagat tatggcagcg gataatacct tctggctcct ggcgtaattt
     1081 tggccaataa gctgtcccat acgatccatc ctctttttta gccctgccgg ccattcgttt
     1141 agtatattgc ctatatcaaa gagggtcatt tcatcgtatg ccggcaggga ggcggccgat
     1201 acaccgcatt tattaaaagg gcataccatc tgacaaatat cacagcccag cattttattt
     1261 ccaatcttat ctctgatgtc cccgggcaca aaatccgaag acagcatatg atgcctaagg
     1321 catttgcttg ggataacccg gccatccgtc ttaatagcgc cgtttggaca ggcacttatg
     1381 cataggtcac aatccccgca atcggttaat gtttccatgc ctgtatcagg gctgaggttc
     1441 gcatcggtca atataaagtg cagacttatc caagacccat atttttcggt atgaataagt
     1501 gaattctttc caaagtatcc tatccctgcc ctcagggcca actccttagc cggtaacggg
     1561 ggatgtacca tagcctcata accgaccttt ttcagaaagc gtccaagctc aaatgcgaca
     1621 tcccggccct tgggatattc cctataatgg gcggagaatc tgcctatgcc ctcgggaaag
     1681 ttggctttat atggggtata tggccatgca gccacgataa cggacttggc tgtcggcata
     1741 atgcgtttgg ggtcatgttc catgttgtcc caaagatgga tcgattcggg atcgtcctct
     1801 ccgcgcccgg aaatttgcga atcccacagc tctaagggct tggcatcggt aaacccaata
     1861 agtccaaacc ccagatccat accatattca attagtcgtt gtttcaatag atccttatcc
     1921 atggtccctc ctaaacaaag gcgggggtcc cttcagcccc tgggaacaag ccccaccttc
     1981 ttatatacct tcctaagggt tttataggaa aggctctccg ccctttgggc accatctttc
     2041 atgatgttat ccaaataggc cttatccgct attagttcat catgcttctc ctgaataggc
     2101 cttagggtct ccgcgaccac ctgccctaca gcggctttaa agtccccata ccctaaacct
     2161 tcaaaatctt tctctatagt cccataggat ttctccgtga tgctggaata aatgctcatt
     2221 aaattgctta tcccgggttt gattttctcg tcatacctga ccatggcctc tgaatcagta
     2281 acggcgcgcc taaatttcct gatgattaca tcaggctcat ccaataggga aatatagcta
     2341 ttgggattat catcggattt ggacatcttc ttttccggct cctgcaggct catgatcctc
     2401 gcccccactt tgggaatata gggctccggt atcctgaaca catccccata tatattgtta
     2461 aacctttggg cgatatcccg ggttatctcg agatgttgtt tttggtcttc cccaacgggg
     2521 acaagatccg cctggtagag caagatatca gcagccatca atactggata ggtaaatagg
     2581 cccgcattta tattatctgc atgtctttgg gacttttcct taaactgggt catcctgcca
     2641 agctcacccg tataggtaaa gc
//
LOCUS       DASRNX010000132         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_450323,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000132 DASRNX010000000
VERSION     DASRNX010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450323"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFD89_05340"
     CDS             <1..239
                     /locus_tag="VFD89_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HZJ57648.1"
                     /translation="IDPVQYIEENKLGMISDTSLINEIIKKVLWENPKAVEEYRGGSK
                     KVQGFFIGQIMRKLGGKANPQMITSLLRDALKED"
     gene            complement(356..913)
                     /locus_tag="VFD89_05345"
     CDS             complement(356..913)
                     /locus_tag="VFD89_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HZJ57649.1"
                     /translation="MGIMKRSQINAAIREAGEAFKRHHWILPPNPKWDVTDFGLGDFD
                     STGLILVNLTEQPEYCEKIMYIKKDQVTPTHYHANKKEDIICRWGVLAVRISSQHESV
                     RLQINGEETDIPTGGPIYLKAGERITMTRGIRHSFWADSQYAITGEVSAANDDVADNF
                     FDDPDISRFSEILEDEPALVRLVSD"
     gene            complement(954..1970)
                     /locus_tag="VFD89_05350"
     CDS             complement(954..1970)
                     /locus_tag="VFD89_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZJ57650.1"
                     /translation="MKQGIALAGNLTVDHIKTVESYPLEGNLTSILSLEKFIGGAPVN
                     VGIGLSKMDNRIPITVTGVVGSDGDGEFIIERLRENGIDINKIKRDSTAVTSFTDVIT
                     AQSTGNRTFFHYRGANELLGVEHFNFSELAKARILHMGYALLLDSLDESDPEYGTVMA
                     RVLAMAQGHGIKTSLDVISEKSDRYSKIIPYSLKHCNYFIVNEFESSQTTGIPHRDNE
                     GRLIVRNMRAMCERLLTMGVNDLVVIHAPEGGFAMDVYGNYYEQPSLKLPKGYIKGAV
                     GAGDAFCAGVLYGLYMDWDIQRALRLAVSAAACCLSQVNATDGMVEVSAAEKLYENMG
                     KQDL"
     gene            complement(2173..3021)
                     /locus_tag="VFD89_05355"
     CDS             complement(2173..3021)
                     /locus_tag="VFD89_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57651.1"
                     /translation="MIKQFPRTEVGGVSLSRMIIGTNWILGYSHTTVAADNLIKKRHD
                     TPEKIANMLEVFLESGVDTIMGPFVNSPILADAVKAAEDRTGKGMIIVDTPIINVDDT
                     PEARAEAEKTIAASKKLGTTFCLPHHSSVEELVNKNKKTIERLPDYLAMVRDHDMIPG
                     LSCHMPELVVYSDLNDYDVETYIQLYNCMGFLMQVEVEYIHKVIWQAKKPVMTIKPMA
                     AGRVSPFVGLTFSWHTIRPCDMVTIGCFTPEEAAEDIEISMAALESRPPELEGRSSPK
                     KTEVMK"
     gene            complement(3078..>3546)
                     /locus_tag="VFD89_05360"
     CDS             complement(3078..>3546)
                     /locus_tag="VFD89_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZJ57652.1"
                     /translation="SEVLYFGDDLGMQQSLPISPERWRKYLKPCYKSLYKYAHDNDRY
                     VYMHSDGHIYPIIPDLIECGVDIINPQYRANGLGNLVEACKGKVCVNLDLDRQMFPFC
                     TPDDIEEHIREAVEKLGSPEGGLMLLAECGPDVPLENIEAICSALEEYSLFFS"
BASE COUNT          922 a          888 c          711 g         1025 t
ORIGIN      
        1 atattgatcc cgtccaatat atagaagaaa ataagctggg aatgatcagc gatacaagcc
       61 tgattaatga gatcataaag aaagtcttgt gggaaaaccc caaggccgtt gaagagtata
      121 gaggcgggag caaaaaggta caaggctttt tcatcggaca gattatgaga aagctgggcg
      181 gaaaggcaaa tccccagatg atcaccagcc tgctgaggga tgcgttaaaa gaggattagg
      241 tataatcttt ttaaggggcg gcagtattta cagatataca ataccgccgc cccttatttt
      301 aatgaattgg aacaacagtt aacttaaatt ctatcccctc tccgccccag cgttatcaat
      361 cgctcaccag tctgaccagt gccggttcat cctctaggat ttcgctgaac ctacttatat
      421 ccggatcatc aaagaagtta tcggctacat catcgttggc cgcagatacc tcgcccgtta
      481 tagcgtattg gctgtcagcc cagaaggagt gcctaatgcc ccgggtcatg gttatcctct
      541 cccctgcttt taaatagatg ggcccaccag tcggaatgtc cgtttcctca ccgttgatct
      601 ggagccttac cgattcatgc tgggacgata tccttaccgc caataccccc catctgcaaa
      661 taatatcttc cttcttgttt gcatgataat gggtaggtgt aacctggtcc ttttttatgt
      721 acataatttt ttcgcagtac tccggctgtt cagtcaaatt aaccaatata agccctgtac
      781 tatcaaagtc cccaagtcca aagtccgtca catcccattt tgggtttgga ggcaatatcc
      841 aatggtgtct cttaaaggct tccccggctt ccctgatagc agcatttatt tgggaacgtt
      901 tcataatacc catacttaaa atccccccct atactttaat tccgacaaag taatcacagg
      961 tcctgtttgc ccatattctc gtagagcttt tcggcggctg atacttcaac cattccgtcc
     1021 gttgcgttta cttgagacag acaacatgcg gcggcgctga ccgcaagcct taaagccctt
     1081 tgaatatccc aatccatata gagtccatac aaaactccag cacaaaaggc atcacccgct
     1141 cctactgccc ccttaatgta tcctttaggc aattttagcg acggctgctc gtagtagttt
     1201 ccataaacat ccatagcaaa tcctccctcg ggagcatgga taaccaccaa atcattaacc
     1261 cccatggtca ataatctctc gcacatggct ctcatgttgc gaactattag tctgccttcg
     1321 ttatccctat ggggtattcc cgttgtctgg gaggactcaa attcattaac tataaaataa
     1381 ttgcaatgct ttaggctgta aggtatgatt tttgaatatc tgtcgctctt ctcgctgatc
     1441 acatccaggg aagtctttat tccatgaccc tgtgccatgg ccaaaaccct tgccattacg
     1501 gtcccgtact ccgggtccga ttcatccaaa gaatctaaaa gcagcgcata gcccatatga
     1561 aggatccgtg ctttggctag ctcactaaaa ttgaaatgtt ctacacctaa aagttcgttt
     1621 gctcctctgt agtgaaagaa ggttcgatta cccgtagatt gcgccgttat cacatcggta
     1681 aaggaggtca ccgcagtaga atccctttta atcttattta tatctatgcc gttttctcgg
     1741 agacgctcta taataaactc accgtcaccg tcacttccta caactccggt aaccgttatg
     1801 ggaattctat tatccatctt cgaaaggcct atccccacgt ttaccggggc accccctata
     1861 aacttttcca aagataggat actggtgaga ttcccctcta atggatagct ttcaacggtc
     1921 ttaatatggt caacagtaag gttaccggct aatgctatac cctgtttcat ttccctaatt
     1981 cccctttatc catgacttat ccaaataggt agaatcattc gcctgaaaag cctatggcct
     2041 ccaatccggt cttctgcact tcttacatga ggggacggtt tggttattag cccaaattag
     2101 gcaatatata cccatataca aattatccag tattgggcag taaacccaat actggatagg
     2161 atcgtttaac ttctatttca ttacctctgt ctttttaggg ctgcttctgc cttccaattc
     2221 tggcgggcgt gattccagtg ctgccatgga gatctcaata tcctcggcag cttcctcggg
     2281 tgtgaaacaa ccgatggtaa ccatatcgca aggtcttatg gtgtgccatg aaaaagtcaa
     2341 ccctacaaag gggctaaccc ggcctgcggc catgggcttt atagtcatga ccggtttttt
     2401 ggcctgccaa atgaccttat ggatgtattc tacctccacc tgcatcaaaa agcccataca
     2461 attgtatagc tgtatatagg tttcaacatc gtaatcgttg agatcggaat atacgaccag
     2521 ctccggcata tgacaggata aacccggtat catatcatga tctctgacca tggccaaata
     2581 gtccggaagc ctttctatgg tcttcttatt tttgttgact agttcctcaa cggagctgtg
     2641 atggggaaga caaaaggtag tacccagctt tttgcttgcg gctatcgtct tttcggcctc
     2701 ggcccttgct tccggcgtat catctacatt gatgatgggg gtatcaacga tgatcatacc
     2761 cttacccgtt ctgtcctcag ctgccttaac cgcatcggct aaaatagggc tgttgacaaa
     2821 ggggcccatg atggtgtcaa caccggattc taggaacact tcgagcatat tcgcaatttt
     2881 ttccggggta tcgtgcctct tcttgatgag attatcggcg gctacggtcg tatgactata
     2941 tcccagtatc caattggtcc cgatgatcat cctggatagg gatacgccac ctacctctgt
     3001 tcggggaaat tgttttatca tggatttttc ctcctaaata attattttat tagaaaaaca
     3061 ataatgaact tcataggtta tgaaaaaaac aggctgtact cttcaagggc gctacagatg
     3121 gcctcgatgt tttctaatgg cacatcgggc ccgcactcgg caaggagcat aagcccgccc
     3181 tcaggtgaac ccaacttctc tacggcttct cttatatgtt cttcgatgtc atcgggtgta
     3241 caaaacggaa acatctgacg atccagatcc agatttacac atactttgcc cttgcaagcc
     3301 tccaccagat ttccgaggcc gtttgcccta tattgggggt ttataatatc aactccgcat
     3361 tcaataagat ccggaataat gggatatata tgcccatcgc tatgcatata cacatatctg
     3421 tcgttatcat gggcgtactt atatagactt ttataacatg gttttagata tttccgccat
     3481 ctttccgggc ttatgggaag gctctgttgc atgcccagat catcgccaaa atatagtact
     3541 tcagaa
//
LOCUS       DASRNX010000133        15403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_609279,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000133 DASRNX010000000
VERSION     DASRNX010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 15403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15403
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609279"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFD89_05365"
     CDS             <1..681
                     /locus_tag="VFD89_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HZJ57653.1"
                     /translation="MGVEVDKPIINEKTLAWNYTNEGGVDGTYRLLKNIMGLWIIQEC
                     KREWDRRGDVYGYDEMADMASAAMPFKAFIDPDHDSFYSPGEMPKKIQKFCRDTGQWI
                     PETKGEILRCVFESLAMKYRWSLEKLEEITGKGLDVLHIVGGGSQNELLNQLTANAIK
                     RPVICGPVEATAIGNLMVQAMALGEVANLAEIREVVKRSFPTEDYLPKDKGAWDSAYE
                     EFLKYNGG"
     gene            711..1094
                     /locus_tag="VFD89_05370"
     CDS             711..1094
                     /locus_tag="VFD89_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019637643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HZJ57654.1"
                     /translation="MGNTKPGGERVWYIPDCYYPEITTEGHYVSHEAICVLNTGLEDA
                     NLSITLYFEDREPIKNFKAKCRAERTNHIRLDMIEDEEGNRIPKGVPYAVMVESNVPI
                     IVQYSRLDTTQAEMGLMTTMAYPLR"
     gene            1495..1695
                     /locus_tag="VFD89_05375"
     CDS             1495..1695
                     /locus_tag="VFD89_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZJ57655.1"
                     /translation="MKNRLEELRRQKGINQEDLALALEVSRQTISSLENGRYNPSIIL
                     AFKIARYFEMSIEEIFIYEEEK"
     gene            1695..2132
                     /locus_tag="VFD89_05380"
     CDS             1695..2132
                     /locus_tag="VFD89_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57656.1"
                     /translation="MLCRYIRSARVWSMLVAMGVVTMVFGILYSRGLPQDLHSMNMLM
                     GMFTGIGGAFALVGGIKLVHYKRASADELKLEEIERNDERNIQLIRMAAVTSNNAATI
                     LFIMMIFILLWLDYRVPALIVLGALYVQSIAFFAAYRYFKNRI"
     gene            complement(2295..2954)
                     /locus_tag="VFD89_05385"
     CDS             complement(2295..2954)
                     /locus_tag="VFD89_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HZJ57657.1"
                     /translation="MYNLIIKDILVQKKSLLFALGYCFFLVFAFQSLEGVTPIAASTV
                     VVYFLIQNSFAYEDKNKSEIIINSLPISRKEIVLAKYLSIFVYIGLAIVAYMIATLTV
                     KVVRVPVKVEFPDLKDVTITLLLVSLMNSIYMPIIFRIGYLKAKIFNMVMFLLFFFIP
                     MGVASLYENPKYSAGISDITGIISSFSDWQIAALLAAISLMLLFFSYSISLGIYKNRE
                     F"
     gene            complement(2947..3813)
                     /locus_tag="VFD89_05390"
     CDS             complement(2947..3813)
                     /locus_tag="VFD89_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ57658.1"
                     /translation="MENILEVTRLKKSFKDFTLKDISFALPRGYIMGFIGPNGAGKST
                     TIKLIMNLLKKDGGQIKVFDKDHIKYEKEIKNRIGFVYDENHFYEELTTREMKSVIAP
                     FYESWDDKTFDRYMKHFKLSTNKKIKELSKGMKMKYSLAMALSHNPELLIMDEPTSGL
                     DPIVRSELLDILGDLIQDENKSVFFSTHITSDLDKVADFVTFIHDGSIILSATKDDIL
                     DNYGIVKGGMDLLNDQTQKVFIGIRKNNYGFEGLVDDRKKARNLFRDNIVIDKPSLED
                     IMVYTVRGDQHV"
     gene            complement(3816..4190)
                     /locus_tag="VFD89_05395"
     CDS             complement(3816..4190)
                     /locus_tag="VFD89_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZJ57659.1"
                     /translation="MKIIILNSSDEPIYRQIVRQLEEMILKGRLVAGDALPSIRGLAR
                     ELHISVITTKRAYEELEREGFIETVPGKGSFVAAQNRELLREKRLDIVEERLIGAILA
                     AKSLDISQDELINLLHQLYEEV"
     gene            4427..5707
                     /locus_tag="VFD89_05400"
     CDS             4427..5707
                     /locus_tag="VFD89_05400"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HZJ57660.1"
                     /translation="MLSIYNERDINKGYERAKELYASLGVDTEKVMEDIKKIPISIHC
                     WQGDDVGGFEVEADGVLGGGILATGNYPGKARNAVELRQDLDKAMSLIPGKHRVNLHA
                     SYAETGGKFVDRDEIAPEHFENWVAWAKEKGIGLDFNPTFFSHRMADSGYTLASKDKE
                     VRDFWIRHAKRCREIGAYMGKALGTPCVNNIWIPDGSKDLPADRLGHRQILKDALDEV
                     LEKKYDREYLLDAVECKLFGIGSEAYVVGSHEFYMGYAITRDTLLCLDAGHFHPTETV
                     SDKISSLLTYIDELLLHVSRGVRWDSDHVVILSDEVMAIAQELKRCDAFHRVHFGLDF
                     FDASINRISAWVTGTRASLKAVMLALLEPTHLLVEAEDNGNFGRRLALMEEFKSLPFG
                     AVWDKYCYDQGVPVGAEWLNDVDEYEEQVLSKRG"
     gene            5722..6771
                     /locus_tag="VFD89_05405"
     CDS             5722..6771
                     /locus_tag="VFD89_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZJ57661.1"
                     /translation="MDLGGLKELERISQDVGGHPDLVQGGGGNTSAKLGDRFMAVKAS
                     GYRLDQVTELDGYVIVDYQRVRDYYKDVDRSRDVDFERESAGFMKECVLKERNPKDLR
                     PSIETGFHSFMDKYVIHTHSVYANILCCSKEGKDVAESIFGDGFKMLWVPYTDPGFEL
                     TIALNDSIRQYEKEKGYTPNIVFMENHGLIVSAGDAEECLRLHEKVNAAIIDYFNLGA
                     IYPEIEIEDIGERGYRSKTSYLQDTLKKRAVGSRLFKEVILYPDQLVYLGDNVSFEGT
                     GKVNVDPDTGEVVYRTNEVEAVTIDETFTAYIYVVDKINELGLTLQTMTEEGISFIRN
                     MEGERYRKSLLKKDS"
     gene            complement(7126..8055)
                     /gene="fba"
                     /locus_tag="VFD89_05410"
     CDS             complement(7126..8055)
                     /gene="fba"
                     /locus_tag="VFD89_05410"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HZJ57662.1"
                     /translation="MPLVTTTEMFKKAYEGGYAIGAFNVNNMEIIQGITEAAKAENAP
                     LILQVSAGARKYANHTYLVKLVEAALEETDLPIALHLDHGDSFELCKSCIDGGFTSVM
                     IDGSHLPFEENIKLTKQVVDYAHDHGVVVEGELGRLAGIEDAINVSEEDASFTDPDEV
                     QEFVEKTGVDSLAIAIGTSHGAFKFKGEARLRFDILEEIGRRLPGYPIVLHGASSVPQ
                     EYVEIINKFGGDMPGAKGVPEEMLRKAASMAVCKINIDSDLRLAMTSTIRKFMAENPG
                     AFDPRQYLAPARDAIRTIVRHKIVNVLGCNDKI"
     gene            complement(8199..9623)
                     /gene="murC"
                     /locus_tag="VFD89_05415"
     CDS             complement(8199..9623)
                     /gene="murC"
                     /locus_tag="VFD89_05415"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZJ57663.1"
                     /translation="MKHIDIKDIKGGRVHFIGIGGISMSGLAEFLLQKGYTVTGSDVM
                     DSYITRDLEGKGIKINIGHQGSSVIGADLVVYTSAIVTSGENPEVSEAKKRDIPLMNR
                     AVLMGQIMRDFPNAIGVAGTHGKTTATSILSLIMTEAQLDPTIFVGGKLDEIGGNVRV
                     GQSPHFLVEACEFSGSFLEMNPYIAVILNIDNDHLDYFKDMDEIYYTFMEFASLVPSR
                     GFVIGNNDDPMVKRLLDEIDNRTISFSIDGKADWVARELEYNSMGYASFRAGYKGQDM
                     GKFTLNVPGKHNVYNALAAIATAWASGIALEAIKRGLASYKGTHRRFEIKGVVEGVTV
                     IDDYGHHPTEIKATLDAAQNYPHKKLWCVFQPYTFSRTKLLFDDFVDSFHGADEVIIT
                     DIMGGRELDTGLIHALDLVKAIKATGQPCRYLPTFDDVVDFLSSNARDGDVVLTTGCG
                     NVYLAAEMLIQRMEETLVAAGLET"
     gene            10165..11826
                     /locus_tag="VFD89_05420"
     CDS             10165..11826
                     /locus_tag="VFD89_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZJ57664.1"
                     /translation="MDIREAMAGGVLFFDGAMGTMIQNAGLKAGEQPEVYNMIHPEII
                     KSIHRSYLRAGADIITTNTFGANEYKLKGTGYTVEDIVERAVSLAKAVVGNGWVALDI
                     GPIGQLMEPSGTLSFDEAYRAVERQVRAGEKAGADLVIIETIADLYEMKAAVLAVKEN
                     SSLPIFCTLTYDGNGRTMMGTDPKAAINVLEGLGVDALGANCSLGPKELLPIVDELLK
                     YAHIPVIIQPNAGLPRLENGKTVFDTDSGEFAAFGKRMIEKGVRIIGGCCGTTPDFIR
                     ELKDRVKDIKISKSGVKPHRHTAVSSASNTVTIGGGVRIIGGRINPTVGESVEKALSE
                     GDFDEVVSEAIEQKMAGADIIGINLGGDKSGANLEEAVREVQGLVNTPLLIDSSDPHA
                     IEAAIRIYNGKGMIKGVDGGMDSMELIFPIAGKYGACVIGRTAYGAEVPQGADKRVKI
                     AESILDKAVEYGIPREDILIDPLAVNGPIHREMYQETIRAIKEIKARFGLKTVLAIGN
                     CSTEAADRDKEWIRLFLTEAIEAGLDCVIIDPLDEGMMEVIEPYR"
     gene            complement(12022..12531)
                     /gene="nrdG"
                     /locus_tag="VFD89_05425"
     CDS             complement(12022..12531)
                     /gene="nrdG"
                     /locus_tag="VFD89_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HZJ57665.1"
                     /translation="MEDTIKIAGIIEESIVDGPGIRMVIFAQGCPHRCVGCHNPETHD
                     FNGGYLMSLEDIALMPSKNPLLDGITFSGGEPFSQAKAFSLLGRRFKDLGLDIMTYTG
                     YSYESLLEGSNDSNAWKQLLDVTDILVDGKFEVEKKNLLLPFRGSENQRIIDMNGTRR
                     KKVVVTLDI"
     gene            complement(12561..14879)
                     /locus_tag="VFD89_05430"
     CDS             complement(12561..14879)
                     /locus_tag="VFD89_05430"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HZJ57666.1"
                     /translation="MITRIRKRDGREVPFNVEKIANAIFKAAMASGGKDYDMALSLAY
                     EVANTLNQTMKGAIPTVENVQDIVEKVLIENGHARTSKAYILYRAERTRIREMNTRLM
                     KVMEELTFKDAKDNDLKRENANINGDTAMGTMLKYGSESAKQFYNMFVLNPEHSAAHR
                     AGDIHIHDLDFFTLTTTCCQIDLGKLFRGGFSTGHGYLREPNSIQSYSALACIAIQSN
                     QNDQHGGQSIPSFDYDMAPGIIKSYKRLYTQNLMKSLELLLGEAYDESKILAVSNRLE
                     KEHQLIPRLESDDEYIDLEGKLLIELLGDEPTIERAQKFAKSKALKEISRETYQAMEA
                     LIHNLNTMHSRAGAQIPFSSINYGTDTSPEGRIVTKNLLLAAEAGLGNGETPIFPIHI
                     FKVKEGVNYNPGDPNYDLFRLACRVSAKRLFPNFSFLDAPYNLKYYKPGHPETEIAYM
                     GCRTRVVGNVYDPSQEIVNGRGNLSFTTINLPRLGLKHPNDLYAFYEDLDAKIAMVVE
                     QLLERFEIQAKKKVKNFPFLMGQHLWLDSHKLGPNDEIREVLKHGTLSMGFIGLAECL
                     KALIGEHHGESERAQKLGLEIINHMRRRMDEVSQSLNINFTLLATPAEGTAGRFVKMD
                     REVYGSIPGITDREYYTNSFHIPVYHSISAFDKIGLEAPYHELTNAGHITYIELDGDP
                     LNNLGAFEKIIRTMKEAGVGYGSINHPVDRDPVCGYTGIIGDTCPSCGREEGDAKFER
                     IRRITGYLVGTIERFNDAKQAEEHDRIKHLGV"
BASE COUNT         4245 a         3234 c         3710 g         4214 t
ORIGIN      
        1 atgggggtag aggtcgataa gcccattata aacgaaaaga ccttagcgtg gaactacacc
       61 aacgaaggcg gggtagacgg aacctacagg cttttaaaaa atatcatggg cctgtggatt
      121 atccaggaat gcaagaggga atgggatcgc aggggcgatg tttatggcta tgacgagatg
      181 gccgatatgg catcggctgc tatgcccttt aaggccttta tagacccgga tcacgacagc
      241 ttttacagcc cgggggaaat gcctaaaaaa attcaaaagt tctgcaggga tacggggcag
      301 tggattccgg aaaccaaagg tgagatttta agatgtgtat tcgaaagttt ggccatgaaa
      361 tatcgctggt cgctggagaa acttgaggag attacgggta aaggtctgga tgtcctccac
      421 atcgttggcg gaggtagcca gaatgaactt ttaaatcaat tgaccgccaa tgccataaaa
      481 aggccggtca tatgcgggcc cgttgaggcg acggcaatag gtaatcttat ggttcaggct
      541 atggcccttg gtgaggtagc aaacctagca gaaatccgcg aagtcgttaa gagatccttt
      601 cccacggaag attatttgcc caaagataaa ggggcatggg atagcgcata tgaggagttt
      661 ttaaagtata acgggggata attttattga aataagaaag ggtggggtaa atgggaaaca
      721 caaagcctgg gggagaacgt gtttggtaca tccctgattg ctattatccg gaaataacaa
      781 ctgaggggca ctatgtaagc catgaggcca tatgcgtttt aaatacaggt ttagaggatg
      841 ccaacctcag tataacccta tactttgagg accgggagcc tataaaaaac tttaaggcaa
      901 agtgcagggc ggagagaaca aaccatattc gcctggatat gatagaggat gaagagggta
      961 acaggatccc taaaggtgtc ccctatgccg ttatggtaga gagcaatgta ccaataatag
     1021 tgcagtacag ccgattggat accacccaag ccgagatggg gctaatgacg actatggctt
     1081 atcccctcag atagagaccc cctcagatag ggcaagcggg cgggcgaaat ttccacggat
     1141 taaaacaggt gaaacctgaa gatcgttatc tgccggtatg ggatggggcg gataaggtgt
     1201 tttgtatatt cgaatagata gaatgattca gatatcaaaa ggtcataatt caattttgta
     1261 ccatcatata tagctcaata cattagtgtt ataactattt atggtatgga ttgtttgcaa
     1321 aatagggttt tgacatccta atcatagaat tttattttta attggactaa aggcgggata
     1381 accgctcttt tttttaatta tttacgtaaa gcgtacttga catagcgata ggcaacaggt
     1441 atactgatgt tgtaaagtta actttacaac gaatataggg aggtgtggct atttgtgaaa
     1501 aacaggctgg aagaactcag gcgccaaaaa ggcatcaatc aggaggatct ggctttggca
     1561 ttggaggttt caaggcaaac gataagttca ctggagaatg ggcgatacaa cccatcaatt
     1621 atcctggctt ttaaaatagc ccggtatttt gaaatgtcaa tcgaagaaat ttttatttat
     1681 gaggaggaaa agtaatgctg tgtagatata taaggagcgc aagggtttgg tcaatgttgg
     1741 ttgctatggg tgttgtaacc atggtatttg gtatattata ttctagggga ttgcctcaag
     1801 accttcatag catgaatatg cttatgggga tgtttaccgg tataggcggc gcattcgccc
     1861 ttgttggagg catcaaatta gtccattata aaagggcttc ggcggatgaa ttaaagctgg
     1921 aagagataga acgaaatgat gagagaaata tacaattaat caggatggcc gctgttacct
     1981 caaacaatgc agctacaatt ctcttcatca tgatgatatt catactcctt tggctggatt
     2041 accgggttcc tgcgcttata gtgttggggg ctttgtatgt acagtcgatt gcattttttg
     2101 cagcatatag gtattttaaa aataggatat aataaatgct attaaggtaa taaaaacttt
     2161 atggttttat ctgatgcatt tattgaatcg gtgtgtggca agccaattaa ggcttgctcc
     2221 tatgatataa ttaattgccg cccaaacata tacgggcgag tatcataggc gacgttgata
     2281 cccgcccatt tgggttagaa ttcacggttt ttatatatcc ctagggagat agaatatgaa
     2341 aagaatagga gcatcaggga tatagcggcc agtagcgcgg caatctgcca atcagaaaac
     2401 gatgagatta tgcctgttat atcgctaata cctgcgctgt attttggatt ctcgtagagg
     2461 ctggccaccc ccatgggtat aaaaaagaat agtaaaaaca tgaccatgtt aaagatcttt
     2521 gcctttagat aaccgatcct aaatattatt ggcatgtata tggaattcat taaggatacc
     2581 agaaggagag ttatggttac gtccttaaga tcaggaaact ccacttttac aggaaccctt
     2641 accaccttta cggtaagggt ggcgatcata taggctacaa tagctagacc tatgtagaca
     2701 aatatagata agtatttggc aagaactatt tccttcctgc taataggaag gctgttgata
     2761 atgatttcgg atttgttctt atcctcatag gcaaaggaat tctggattaa aaagtagact
     2821 acaacggtag aagcagcgat gggagtcacg ccttcaaggc tttggaaagc aaataccaag
     2881 aaaaagcaat agcccaaggc aaatagtaag gattttttct gtactaatat gtccttaatg
     2941 atcagattat acatgttggt cccccctaac tgtgtatacc atgatatcct caaggctggg
     3001 cttatctatg actatattgt cccggaacag attacgagcc tttttcctgt catcgaccag
     3061 gccttcaaat ccataattat ttttgcggat gcctataaaa actttctggg tttggtcatt
     3121 caataaatcc ataccgccct tcacgatgcc gtagttgtcc aggatatcat ccttagtagc
     3181 tgatagaatt atcgacccgt cgtggataaa ggttacaaag tctgctacct tatccagatc
     3241 agaggttata tgggtggaga aaaacacgct cttattctcg tcctgtatca aatcacccaa
     3301 tatatccaat agctcgctgc gaacgatggg atcaagtccg gaagtcggct catccatgat
     3361 aaggagctcc gggttatggg atagagccat ggccagagaa tacttcattt tcattccctt
     3421 tgacagctcc tttatcttct tattcgtaga aagcttaaag tgtttcatat acctatcaaa
     3481 ggttttatca tcccacgatt cgtagaaagg ggctataacg ctcttcatct cacgggtcgt
     3541 cagttcctcg tagaaatggt tttcatcgta cacaaagcct atacgatttt taatttcctt
     3601 ttcatactta atgtggtcct tgtcaaagac cttgatctgt ccgccgtctt ttttaagcaa
     3661 gttcatgata agctttatgg ttgtactctt tccggcaccg ttgggaccga taaaacccat
     3721 gatatagccc ctggggaggg cgaagcttat atcctttaga gtaaagtcct taaaactttt
     3781 cttaagccgt gtcacttcca gaatgttttc cacaattata cctcctcgta caattgatgc
     3841 agcagattta ttaattcgtc ctgggaaata tccagggatt tggctgccaa aatagccccg
     3901 ataagtcttt cttctactat atccaacctt ttttctctca acagttccct attttgagca
     3961 gcaacaaagg agcccttccc gggaaccgtc tcgataaatc cttccctttc cagctcttca
     4021 taggcccttt tagtggtaat gacgcttata tgtagttctc tggcaaggcc ccggatagag
     4081 gggagagcat caccggctac aagacgcccc ttcaggatca tctcctctaa ttggcggact
     4141 atttgccggt agataggttc gtctgatgaa ttcaaaatta ttattttcat tagcgcctcc
     4201 atggtatgca taccgtgcat atacaatata tacagctaat aaagctttgt caaggggaaa
     4261 tttcccataa tgcgggatac taaatcagtg ccattttata taaattcttc ccaataaaga
     4321 ggatattttc ttgcagcgta gaataaagaa gttagaatag agaattagaa tgaagacgaa
     4381 taaactgtcg gattacctca aattttaatt tataaggggt gttaaaatgc taagcatcta
     4441 taacgaaagg gacataaata agggttacga acgggccaag gaactgtatg cttcccttgg
     4501 ggtggacacg gaaaaggtca tggaagatat taagaagatc ccaatctcca tccactgctg
     4561 gcagggcgac gatgtgggcg gatttgaagt ggaggcagat ggggtcttag gcggaggaat
     4621 tttagccacg ggcaattacc cgggaaaagc acgtaatgcc gtagagcttc gccaggattt
     4681 agataaggcc atgagcctta tacccggaaa gcatcgggta aacctccatg ccagctatgc
     4741 agagacgggg ggcaaattcg tcgatcggga tgagatcgcc cctgagcact ttgaaaactg
     4801 ggtcgcttgg gccaaggaaa agggaatagg ccttgatttt aatcctacct tcttctccca
     4861 taggatggcg gactcgggct ataccttggc aagcaaggat aaagaggtca gagacttttg
     4921 gattcgccat gcgaaaagat gcagggaaat aggagcctat atgggtaagg cccttggcac
     4981 gccctgcgtt aataatatat ggattcccga cgggtccaag gatttacccg ccgacaggtt
     5041 aggccatcgc caaatcctta aagatgcctt agacgaagtc ctagagaaaa agtatgacag
     5101 ggaatatctg ctggatgcag tggaatgtaa gctctttggg atagggtccg aagcctatgt
     5161 agtagggtct cacgaatttt atatgggcta cgccataacc agagataccc tgttatgcct
     5221 agatgctggg cactttcatc ctacagagac cgtttcggat aagatatcat ccctgctgac
     5281 ctatatagac gaactgctct tacacgtgag ccgcggggta aggtgggaca gcgatcatgt
     5341 agtgatactc agcgatgaag ttatggccat cgcccaggaa ctgaagaggt gtgatgcctt
     5401 tcatagggta cattttggtc tggacttctt tgacgccagt ataaatagga tatcggcctg
     5461 ggtaaccggg accagagcat cgttaaaagc cgtcatgttg gcattgttag aacccaccca
     5521 tctgttggtg gaggctgagg ataatgggaa tttcgggcgg cgcctggccc tgatggagga
     5581 gtttaagagt ctgccctttg gcgctgtatg ggataagtat tgctacgatc agggcgtacc
     5641 cgtaggtgca gaatggctaa atgatgtcga cgagtacgag gagcaggtat tatccaagag
     5701 agggtaagag aggagaaatc tatggatctc gggggattaa aggaactgga acgtatttca
     5761 caagacgtcg gggggcatcc ggatctggtg caaggcgggg gtgggaacac atccgccaag
     5821 ctgggcgata ggtttatggc ggtaaaggcc tccggatatc gtttagatca agtaacagag
     5881 ctggacggtt atgttattgt ggactaccaa agggttaggg attattataa ggatgtggac
     5941 agaagcaggg atgtagattt tgaaagggaa agtgccggct ttatgaaaga gtgcgttctt
     6001 aaggagcgaa accccaagga tcttcgcccc tccatagaga cgggattcca ttcctttatg
     6061 gataaatacg tcatccacac ccattcggta tacgccaata tattgtgctg ctcaaaagag
     6121 gggaaagacg tagcagaaag catttttggg gatgggttta aaatgctgtg ggtcccctat
     6181 accgatccgg gatttgagtt gaccattgca cttaacgatt ccatccgcca atacgagaaa
     6241 gagaagggtt acacgccgaa tatagtcttt atggaaaatc acggtcttat agtatcggca
     6301 ggggacgctg aggaatgcct aaggcttcac gaaaaggtta atgcggccat catagattat
     6361 tttaatttag gggcgatata tccggagatt gaaatagagg atataggcga gaggggatat
     6421 aggagcaaaa cctcttacct acaggatacc ctcaaaaaaa gggccgttgg atccagatta
     6481 tttaaagagg taattctcta cccggatcag ctggtctatc tcggagacaa cgtctccttt
     6541 gaaggaacag gaaaggtgaa tgtagaccct gatacaggcg aggtggtcta taggacaaat
     6601 gaggtagagg ccgttaccat agacgaaact ttcacagctt atatctatgt ggtggataag
     6661 attaatgaac taggccttac tcttcagact atgaccgaag agggaatttc gtttatacgc
     6721 aatatggagg gggaaaggta ccgcaagagc cttttaaaga aggattctta gtcccgtaat
     6781 aaagatgggc ggaagttcat ctttaccggg aaggttcacc ctatataggg caataaggcg
     6841 atcagtacta cagaaaacct ataggcaaat taaatcagtc ggaatgataa tcctaacgga
     6901 tctatgtaat ctaattacga taattttatt attaggatta tccagctgta aacatgaata
     6961 ttattaacca taggaaatat atggttttaa ggggttaatg ggtattaaaa aggagcgatt
     7021 cttacaccgc tcctttttaa tgttctatta taatgtcctg ttataactat ttagttacgc
     7081 atttcatttc ttcaatgtgg cagatattca tacccaaccg aagatttaga tcttatcgtt
     7141 acaccctaat acgtttacaa tcttatggcg tacaatggtt ctgatagcat ccctggctgg
     7201 ggctaagtac tgtcttgggt cgaaggcccc gggattttca gccatgaact ttcggatggt
     7261 agatgtcata gctagcctta agtcggaatc gatattgatc ttacatactg ccatggaagc
     7321 ggcctttcga agcatctctt cgggaacacc cttggctccg ggcatgtcgc cgccgaattt
     7381 attgataatt tctacatatt cctggggaac ggaggaggca ccgtgtaaaa cgatggggta
     7441 tcccggcagt cttcttccga tttcttcgag aatatcgaag cgaagccttg cctcaccttt
     7501 gaacttaaag gcaccgtggc tggtcccgat ggcgattgcc agggagtcaa ctcctgtttt
     7561 ttcaacgaat tcctgtacct catcgggatc tgtaaaggat gcatcctctt ccgatacgtt
     7621 tatggcatct tcaatacctg caagccttcc cagttcaccc tctactacaa caccgtgatc
     7681 gtgggcataa tctactacct gcttggtaag tttgatattt tcttcgaagg gaagatggga
     7741 accgtcaatc atgactgagg taaatccgcc gtctatgcag gatttacata gctcgaagct
     7801 gtcaccgtga tctaaatgca gggctatggg tagatcggtt tcctcaaggg ctgcttctac
     7861 caatttaacc agataggtat ggttggcata ttttctcgct ccagcggaaa cttgaaggat
     7921 taggggagcg ttttcagctt tggcagcctc ggtaatgcct tggataattt ccatgttgtt
     7981 gacattgaag gcacctatgg cgtatccgcc ctcataggct tttttgaaca tctctgtggt
     8041 tgttactaat ggcatatata ttcactcctt aaaaatttat atgtatagac ttaagttgtt
     8101 caagttcatt ctatcaaatt cactgaaaaa atcaagaagg agccccaaag aattaaaaac
     8161 ccgcgaactt taattgagtc tcgcgggatt tattgtcatc atgtttcaag acctgccgca
     8221 accaatgtct cttccatcct ctggatcagc atctctgccg ccaggtagac attgccgcac
     8281 cctgtagtga gaaccacatc gccgtcccgg gcattggatg aaagaaagtc tacaacatcg
     8341 tcaaaggttg gaaggtatct gcagggttgc cccgtggcct ttatggcctt taccagatcc
     8401 agggcatgta tgagtccggt atccagctcc cttcctccca tgatgtcggt gattatgacc
     8461 tcatcggctc catgaaatga atctacaaaa tcgtcaaaga gcagcttggt ccgggagaag
     8521 gtatagggct ggaatacaca ccataacttt ttgtggggat aattctgggc ggcatccaaa
     8581 gtagccttta tctccgttgg atggtgaccg taatcgtcta taaccgtaac tccttctact
     8641 acacctttta tctcaaatct cctatgggta cctttatagg atgctaggcc gcgcttgatg
     8701 gcctctaggg caatccccga tgcccaggcc gttgcaatag ccgccaaggc attgtagacg
     8761 ttgtgtttgc ccggcacatt taaggtaaac ttacccatat cctggccctt gtacccggcc
     8821 ctaaaggatg catagcccat ggaattgtac tctaattccc tagctaccca atcggcttta
     8881 ccatcgatgc tgaagctgat ggttctatta tcgatttcat ctaggagcct tttgaccatg
     8941 ggatcatcat tgttacctat gacaaaccca cggctgggga ccagactggc aaattccatg
     9001 aaggtataat agatctcatc catatcctta aaatagtcca ggtggtcgtt atcgatattt
     9061 aaaataacag ctatataggg gttcatctcc aggaagctac cagaaaactc gcaggcctct
     9121 acaagaaaat gcggactctg gcctactcta acattacccc cgatttcatc cagcttcccc
     9181 ccgacaaaga ttgtggggtc taattgtgct tcagtcatta taagggatag tatggaggtg
     9241 gcggtagttt taccatgggt acctgcaacc cctatggcgt tgggaaagtc cctcataatc
     9301 tggcccataa gaactgccct gttcataagg gggatatccc tcttcttggc ctcagagacc
     9361 tcggggtttt cgccgctggt tacaatagca gaggtatata caaccaagtc ggcacctatt
     9421 acgctggacc cctggtggcc tatatttatt ttaatgccct tcccttcaag atcccttgtt
     9481 atatacgaat ccataacgtc ggagcctgta acggtatatc ccttttgaag gaggaattcc
     9541 gccaggccgc tcatactgat gcccccgata cctataaaat ggacccttcc accttttata
     9601 tcctttatat ctatgtgttt cataaatctc accccacaat tcgtatctta ataatattgt
     9661 caattcaagc accatcaccg atatatgctt aacagtcata attaatatca cttgtccatt
     9721 atactatctt tgtatccatt tttcaacgga acctaaaaag ctgtaaataa agtttaacaa
     9781 cccgtaatat ttgaaacacg atatccctaa ggcgacaatc cctttccgga tatagttttt
     9841 tatctatccg taggtcagaa attctaaaaa tatgctgtgt ctcggatcat tatgttgaca
     9901 agcgagcttt tttgaaactt cagctgatgg ggtatttatt tgacgatacc ttatgttata
     9961 atgttcttgt ctaaaccgtg ggttaatcca cggctgatat attaatatcg gtttgtctga
    10021 gggattataa ccattagagc acggtgaaat acgagaatat ggtaaaatgg ggtaatacgg
    10081 tagaatcgga taataatgcg aaatcggata atacagtgca atcggataat acggtgaaat
    10141 tgaataataa ggggaggaag caatatggat ataagagagg ctatggcggg gggcgtgctg
    10201 ttcttcgacg gtgccatggg cactatgatc caaaatgccg gtctgaaggc gggggagcag
    10261 cccgaggtct acaatatgat acatcccgaa ataattaaat ccatacatag gtcctatttg
    10321 agggccgggg cggatataat aaccaccaac acctttgggg ccaacgagta caaattaaaa
    10381 gggacggggt atacggtaga ggacatcgta gaaagggcgg tatccttggc caaagccgtc
    10441 gtagggaacg ggtgggtggc ccttgatatt ggacctatag gccaattgat ggaacccagc
    10501 ggaaccttgt cctttgacga ggcatatagg gctgtcgaaa ggcaggtaag ggcgggagaa
    10561 aaagccgggg cggatctggt gataatagag accatagcgg acctgtacga aatgaaagcc
    10621 gctgtattgg ctgttaaaga aaattcatcg ctgcctatat tctgcaccct tacatacgac
    10681 gggaatggga gaaccatgat gggtacagat cccaaggcgg ccataaacgt attggaaggg
    10741 ctcggggtag atgcactggg tgcaaattgc tccctggggc caaaggagct cttgcccata
    10801 gtggacgagc tactgaagta tgcccatata cccgtcatca tacagccaaa tgcgggactg
    10861 cccagactgg agaatggaaa gaccgtgttt gatacggatt ccggagaatt tgccgccttt
    10921 ggaaaaagaa tgatagaaaa gggcgttaga ataatagggg gatgctgcgg cacaactccg
    10981 gattttataa gggagctgaa agacagggta aaggatatca aaataagcaa atctggagtc
    11041 aaaccccata gacatacggc ggtatcatcc gcatcaaaca ccgtaacaat agggggcggc
    11101 gtaaggatta tcggcggacg gataaacccc actgtggggg aatccgttga aaaagcccta
    11161 tcggaagggg attttgatga agttgtatcc gaggctatag agcagaaaat ggccggtgcg
    11221 gatataatcg gtataaattt ggggggagat aaatcaggtg ctaacttgga ggaggcggtt
    11281 agggaggtcc aggggctggt caatacaccg cttttgatag acagttccga tccccatgcc
    11341 attgaggcgg cgatacgaat ttataacggc aagggcatga taaaaggtgt agacggtggg
    11401 atggattcta tggaattgat ctttcccata gcgggaaagt atggagcctg tgtcataggc
    11461 cgaaccgctt atggagcgga ggtaccacag ggggccgata aaagggttaa gatagccgag
    11521 tcgatcctgg acaaggctgt tgaatatgga atacccaggg aagacatatt aattgatcct
    11581 ttggcagtaa atggtcccat ccatagggag atgtaccagg agacaataag ggccatcaaa
    11641 gagataaaag cccgatttgg tttaaaaacc gtgttggcaa ttggcaattg ttcaactgag
    11701 gcggcggacc gggacaagga gtggattcgt ctatttctaa ctgaagctat agaggcaggc
    11761 ctcgactgcg ttataattga tcccctggat gagggtatga tggaggttat agaaccctat
    11821 agataatgca tgagttatat gatggagatt atagaaccct acagatagtg tataagttat
    11881 atgttggaga ttatgtagat gagattatta gtgacagaag tgaatacttt cagaattgtt
    11941 aatagctgaa acggacatcc tcaaggatcg taaataattg taatggatat ccccaatatc
    12001 gatgattaga cccggggtaa gtcaaatatc cagggttacc accacttttt ttctgcgggt
    12061 cccattcata tcgatgatcc gttggttttc ggaaccgcgg aaggggagaa ggaggttttt
    12121 cttttcaact tcgaatttcc catccaccaa aatatccgta acgtccaata attgcttcca
    12181 ggcgttacta tcattggagc cttctaagag ggactcatag gaatatccgg tatatgtcat
    12241 gatatccagg ccaagatctt taaaccgtct tccaagcagg gagaaagcct tggcctggga
    12301 aaagggttcc ccgccgctga aggttatgcc gtctagcagc gggtttttac tgggcatcaa
    12361 ggctatgtcc tctagggaca tcaggtagcc cccattaaag tcatgagttt ctggattatg
    12421 acatcctaca cacctatggg ggcagccctg ggcaaatatc accattctga tccccggacc
    12481 gtccacgatg gattcttcta taataccggc tattttaatg gtatcttcca aggtttctcc
    12541 cccctttaat aattgtcggt tcaaacccct aaatgcttta tcctatcatg ttcctcggcc
    12601 tgtttcgcat cattgaagcg ttctatggtt cccaccaagt agcctgtgat ccttcggatt
    12661 ctctcgaatt ttgcatcccc ttcttctctg ccgcaggagg gacaagtgtc cccaatgata
    12721 cccgtatatc cgcatacagg atccctgtct acggggtgat ttatagagcc gtaacctact
    12781 cctgcttcct tcatggtcct tataatcttc tcaaaggcgc caagattatt gaggggatcc
    12841 ccgtccagtt cgatataggt tatgtggccg gcattggtca attcgtgata gggggcttcc
    12901 agcccgatct tatcaaaggc acttatacta tggtatacgg ggatgtggaa actgttggtg
    12961 taatactctc tgtcggtaat gccggggatg cttccatata cttctcggtc catcttaaca
    13021 aacctgccgg ctgtaccttc cgccggggtc gccaataggg tgaagtttat atttagggat
    13081 tggctcactt cgtccatgcg ccttcgcata tggttgataa tctccagccc cagcttttgt
    13141 gcgcgctccg attcgccatg atgctcccct atgagggcct ttaggcactc ggccaggcct
    13201 ataaatccca tggagagggt tccatgcttt aagacctccc ggatttcgtc attggggcct
    13261 aatttatggg aatccagcca gaggtgttgt cccatcaaga agggaaagtt cttaaccttc
    13321 ttcttcgctt ggatctcaaa ccgttccagt aattgctcca ccaccatggc tatcttggca
    13381 tccagatcct catagaaggc gtacagatcg ttggggtgtt tcaatcccaa cctgggaaga
    13441 ttgatggtgg taaagctcag attgccacgc ccgtttacaa tctcctggga ggggtcatac
    13501 acattgccga ctaccctggt tctgcatccc atatacgcga tctctgtttc cgggtgccca
    13561 ggcttataat actttaaatt atagggtgca tccaaaaagg agaagtttgg aaacagcctt
    13621 ttggcgctta ccctgcatgc caaccggaac agatcatagt ttggatctcc cggattatag
    13681 ttaaccccct ctttaacctt gaatatatgt attggaaata taggggtttc accgttgccc
    13741 aggcccgcct cagcggccaa caacagattc ttggtgacga ttctgccctc gggcgatgta
    13801 tcggtcccgt agtttatgga gctgaagggt atctgggcac ctgcccggct gtgcatggtg
    13861 tttagattat gtataagagc ctccatggct tgataggttt cacgggaaat ttccttgaga
    13921 gccttggatt tcgcgaactt ttgagccctt tcgattgtag gctcgtcccc caacaattca
    13981 atgaggagtt ttccctcaag gtcaatatac tcatcgtccg attccaacct ggggattaat
    14041 tggtgttctt tttccaaacg gttggagaca gccaggattt tggattcgtc ataggcttcc
    14101 ccaaggagaa gttccaggga cttcataaga ttttgggtat agagcctttt gtaggatttt
    14161 attatgccgg gggccatatc atagtcaaag ctgggtatgc tctggccgcc gtgttgatcg
    14221 ttttgattgg attgtatggc tatacaggcc agagcagaat agctctggat gctgttaggc
    14281 tctctgagat aaccatggcc ggtgctgaaa ccgcccctga agagttttcc aagatcgatt
    14341 tggcagcagg tggtggtcag ggtgaaaaag tccaggtcat ggatgtgtat atccccagcc
    14401 ctatgggcag cggaatgctc cgggtttaac acaaacatat tatagaattg tttggcgctt
    14461 tccgagccat acttcagcat ggtccccatg gcggtatcgc cgttgatatt ggcattttcg
    14521 cgctttaaat cattatcctt tgcgtctttg aaggtcagct cctccattac cttcataagc
    14581 cgggtattca tctcccttat cctggtcctc tccgcgcgat agaggatgta tgccttggag
    14641 gtacgggcat ggccgttttc gatcaatacc ttttcgacta tgtcctgcac attttcaacc
    14701 gtcggaattg cgcccttcat ggtttgattc agggtatttg caacctcata ggcaaggctg
    14761 agagccatat catagtcttt tccaccgctg gccatggcgg ccttaaatat tgcatttgct
    14821 attttctcca cgttaaatgg cacttcacgg ccatctcttt ttcttatcct ggtaatcaat
    14881 acttttcccc ctttggtagc tttgaccttc tgtagacaac attaccatat accacttata
    14941 ggcgcaaaca tcataaaaga atactacatg ttgtatatag ccaaaataac acccttatat
    15001 ccgattatct tcgttatttg gaagttggag attagaagtt agaagttcgg tcccatagaa
    15061 gcatctttcc atgcaaaata agcatgacgt cttaagccga tcccactggc aggggattgg
    15121 attggcagtg aagggctttt gcgcgttaca taggcccagc tttccaagct caactaatgt
    15181 agtcatgggc tttaaagtag tccattatac ttatcacacg gcaatataat aagccagcat
    15241 aacgcgttaa ttcaaccggt cttcgaattt acagatttta cgtaaaagat ttatacaact
    15301 atttcatgcc cggctaagaa aacgctgcat aagggtatgt ccttccgaga taaatatacc
    15361 cgtatccccg gcctggaatt catcatatcc catagccgaa gtc
//
LOCUS       DASRNX010000134         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_240246,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000134 DASRNX010000000
VERSION     DASRNX010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240246"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1053
                     /locus_tag="VFD89_05435"
     CDS             235..1053
                     /locus_tag="VFD89_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZJ57667.1"
                     /translation="MIHKTISIWDKTGYAYDNTDDFMPTLDTYILGGDIKRPAVLICP
                     GGGYTFTSPREAEPIAMQFNAAGFHAFVLYYSVAPKRHPQPLMDLSRAMCILRQHGDE
                     WKLDTDRIAVCGFSAGGHLAASLGVHWNKPYLEGISGIEPGNNRPNALILCYPVITSG
                     EHAHLGSMENLLGDKISDSHLLKEMSLELHVHKETPPTFLWHTFDDGAVPLENTLLFA
                     QRLRENNIPFELHIYPHGPHGLSLANAETDDGSMGTFPHVATWMGLCIEWLGGL"
     gene            1064..1528
                     /locus_tag="VFD89_05440"
     CDS             1064..1528
                     /locus_tag="VFD89_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HZJ57668.1"
                     /translation="MKILVDADACPVKDIIIKIAKDLELEVIMFIDTSHILDDGYSKV
                     VTVSQGRDAVDIALINQTQKGDIVVTQDYGVASMALGKQAYAINQNGLIYDSTNIDRL
                     LMERYVSQKIRRAGGRTPNHKKRNNRDNLKFERTFTRLCIDALNFGIDTPSE"
     gene            1686..2255
                     /locus_tag="VFD89_05445"
     CDS             1686..2255
                     /locus_tag="VFD89_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZJ57669.1"
                     /translation="MSGKQITWIVIGVVALVLILSGIGGYNNLVNLNEDVSNKLSQID
                     NQLQLRNDLIPNLVATVKGFAAHEEEILKNVSDARAKLAGASSVGQMAEGDAQLTSAL
                     SRLLVVVENYPDLKADANFRQLSDNLSGTENRISVARRDYNESATRYNTAIRRFPTNI
                     MAGLFGFDKVEYFKAQEGAQEVPKVDFGE"
     gene            2268..>2910
                     /locus_tag="VFD89_05450"
     CDS             2268..>2910
                     /locus_tag="VFD89_05450"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZJ57670.1"
                     /translation="MKKTLYLIAFILLLIPINACIAEVTIPSHTQGFYVNDFANVLDS
                     NTEDYILEQSIKLDELSGAQLVVVTVDTTDDRPLEEYALDILRDWGIGSRDKNNGALI
                     FLAVEDRKSRIEVGYGLEGALPDGKTGWIQDEYMVPYFSVGDYSTGILEGYKAILLEI
                     YNEYDIEPQGLENITPSSPNSLDQTEESETSGGVPAILFLIIVFVLFDLLFMRG"
BASE COUNT          831 a          593 c          693 g          793 t
ORIGIN      
        1 ctggagtagg atgcttgccg tagacctaca gaagtttcat atggatttaa tcaaacctgt
       61 tttttgggta tttactataa ttttatttgt gatgcctggc atatatatgt ataattagga
      121 gtgtcagcct ttatattcta actcggtatt cttaagtaat tctacgccta acgccaatat
      181 cctttaaatc tatttgtttt tatagatatt atttacgaaa ggaagagagt ttctatgatt
      241 cacaaaacca tatccatatg ggataagaca ggttatgctt atgataatac agatgatttt
      301 atgcccaccc tagataccta catattgggc ggcgatatca agagaccggc cgtccttata
      361 tgcccgggcg gggggtatac ctttacgtcc cctcgggaag cagaacccat agccatgcag
      421 tttaatgctg cgggtttcca cgcattcgtc ctatactata gtgtggcgcc caaaaggcat
      481 ccccaacccc tgatggattt atcacgcgcc atgtgcatcc ttcgccagca tggggatgag
      541 tggaaactgg ataccgacag aattgcggtc tgcggttttt cggcaggcgg tcatcttgcg
      601 gcaagtcttg gtgtccactg gaataagcct tacctagagg ggatatcggg tattgagcca
      661 ggtaataatc gccccaatgc cctaatacta tgctaccccg taattacttc aggggaacat
      721 gcccatttag gttcaatgga gaaccttctt ggggataaaa tatccgattc gcatctttta
      781 aaggagatgt cgttggaact tcatgtccat aaggagacgc cgcccacttt cctttggcac
      841 acctttgatg acggggctgt accactggaa aatacattgc tttttgctca gcgactccgc
      901 gaaaacaata tcccgtttga attgcatata tatccccatg gtccccacgg cctttccctg
      961 gcaaatgcag aaaccgatga tggtagtatg ggcacttttc cccacgtcgc tacatggatg
     1021 ggattgtgta tagaatggct agggggatta tgatttgcat aggataaaga tattggtaga
     1081 tgcagatgct tgtcccgtta aggatattat cattaaaata gctaaagatt tggagcttga
     1141 ggttataatg ttcatagaca ccagccacat attggatgac ggctattcca aggtagtaac
     1201 ggtgtctcag ggaagggatg ccgtggatat agcccttatc aatcaaaccc aaaaagggga
     1261 tatagtggtt acccaagact atggtgtagc ttcaatggcc ctgggtaaac aggcatatgc
     1321 tattaatcag aacggcctaa tctatgattc aactaatata gatagattgc ttatggaaag
     1381 gtatgtatcc caaaagataa gaagagccgg gggcagaacc cctaaccata aaaagcgcaa
     1441 taatagggat aaccttaagt tcgaaaggac ttttacaaga ctatgtattg atgcgttaaa
     1501 ttttgggatt gacaccccaa gcgaatgaat atatgaaatg ctacagacaa aatattaaat
     1561 tgatctgttg tgctaattca ttaccctact gggtgataat attagtgttc aatagccatt
     1621 accgatatat ttagctggca caattagtaa attgggtgat ttaaatgaag ggagatttaa
     1681 tttttatgag cggtaaacaa ataacatgga tagtaatagg tgtagtagcc ctggtgctaa
     1741 tcttaagcgg cattggaggg tacaacaacc tggtaaactt aaatgaggat gtcagcaaca
     1801 agctaagcca gatagataac caattgcagc tgagaaatga tctgatcccc aacctggtag
     1861 ccacggtcaa gggcttcgca gcccatgaag aagagatact taaaaatgta tccgatgcca
     1921 gggccaaact tgcaggggct tctagtgtag gccagatggc agaaggcgat gcacagctga
     1981 cgagtgccct atcacggctt ttagtggtag tagagaacta ccccgaccta aaagccgatg
     2041 ctaactttag acagctatcg gataatcttt ccgggaccga gaatcggatt tctgttgcca
     2101 ggcgggatta caacgaatcg gcaacccgct ataacaccgc tatcaggcga ttccccacta
     2161 atatcatggc aggactcttt ggctttgata aggttgaata ttttaaggcc caagaaggcg
     2221 cacaggaggt cccaaaggta gatttcggtg aataaggggc aaaaatcatg aaaaaaacgc
     2281 tatatttgat agcttttata ttgctgctga tacccataaa tgcttgtatc gcggaggtga
     2341 cgattccgtc ccatacccag ggtttttatg taaacgattt tgccaacgtc ctagattcca
     2401 atacggagga ctatatactg gagcagagca tcaaactgga tgagctgtcc ggtgcccaac
     2461 tggtagtggt tacggtggat accacggatg accgtccctt agaggagtat gccttggata
     2521 tcctccgaga ttggggaatt ggaagcaggg ataaaaacaa cggggcgctt atcttccttg
     2581 cagtggagga cagaaaatcc cgtattgagg tgggatatgg gctggaaggt gcccttcccg
     2641 acggtaagac cggttggatt caagacgagt atatggtccc ctacttttct gtaggcgatt
     2701 attccacggg tattttggag gggtataagg caattctttt ggaaatttat aatgaatacg
     2761 atatagaacc ccaggggctc gaaaatataa ccccttcttc gccgaattcc ctggaccaga
     2821 cagaggaatc cgaaacatct ggcggcgtac cggctattct tttccttata atcgtatttg
     2881 tgttgttcga tctccttttc atgaggggta
//
LOCUS       DASRNX010000135        10047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_556761,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000135 DASRNX010000000
VERSION     DASRNX010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556761"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1424
                     /locus_tag="VFD89_05455"
     CDS             273..1424
                     /locus_tag="VFD89_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZJ57671.1"
                     /translation="MKYRKFGDLGFDVSVFGVGCMRLPLKVQPDGTTDRTKIDEEEAI
                     KMIRHAIDSGVNYIDTAYPYHGGNSEPLVAKALKDGYRDKVKIATKLPVWQTDSYEDF
                     ERILDEQLAKLEVETIDFYLLHSLSKTSWENAKARGVLDFLDKAVASGKIKYPGFSFH
                     DELPVFKDIVDSYDWKMCQIQLNILDENYQAGVEGMRYAASKNIPVVIMEPLKGGRLA
                     HSVPQDIVDLWNEAGTKRSPVEWAFRWLYNFPEIVVILSGVSTMEQLKDNIEIFSKAA
                     PNSMSNEELELVKRVKDQYDSKIKVGCTACDYCMPCPSGVDIPGIFALYNDYSIYGGA
                     EEFKNRYQEFIEDGKDASNCIECGQCESECPQHISIIEKIQEAHEVLTA"
     gene            complement(1562..4546)
                     /locus_tag="VFD89_05460"
     CDS             complement(1562..4546)
                     /locus_tag="VFD89_05460"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HZJ57672.1"
                     /translation="MKTIDDLIVRRFTKELEAHKDRTVYDLFKWKRVDVILRDYRTNE
                     IINDMRDLEFPVHYSQNACDIIASHYFRKAGLGDHLGYESSMRQVVHRMVSFWIGALV
                     DEGLVDSEYQKEVLYDELVYLLLSQSFAPNSPQWFNTGLKHAYGIEGEPNGLFYYDTD
                     SGEVVESRDLYSRTQASACFIISIEDKLMGDRSISEQYVTETKLFKGGSGTGTNFSTL
                     RAEGEGLSGGGVSSGLLSFLKGFDRNAGAIKSGGTTRRAAKMVIVDDDHPDIMDFVNW
                     KRLEEDKVRALGKMGYDIDFNGEAYNTVSGQNGNNSVRLYDETMKRIMNLDEDPEAVI
                     TLKGRVDQSINKELKVKELWDGINESAYYCADPGLQFHGRFNQWHTCPAGEDGETWAK
                     HNQINATNPCSEYAFLDDTSCNLASINLYQFLDTETGEFRVEDFMHTVALVQLVLEAS
                     IHWGQFPTKHIARKTHMFRTTGLGPANLASLIMSCGYAYDSHEARTMAASIMGIMTGY
                     SYYVSSLMAQKLSPFEKYDINSQHMLRVIRNHGRVAGARDDDYEDLSYKPMEIDHETL
                     KGLGWDNIGEELIRVWDMAYDSGEKNGYRNAQVTVVAPTGTISFAMDCGATSIEPFYA
                     HVIYKKLAGGASMVMVNPVIETALERLGYSEEQIGGILGYILRKDDNDFIADGKIEGA
                     PYLKPQHYSIFDTAGKCGTGNRVISPEGHVLMVSAVTPLISGSVSKTVNLPGSASKED
                     IERIHLLAYTTGTKAIAIYRDGSKASQPLTSSVDNGAGRRLEDMSYGELLDIAKAGRQ
                     GAAVRRKATGRRAGFTHSAKIGDIELYVTVNFYPEGGIAELFISTDKEGTVVKGLLAS
                     LSKAISNMLQYNIPPKEIARMLRGQQYEPSGFVSRHPYIKHASSISDLVSKIIDIELG
                     NFSRCEAKPGMDGTILSARDLVTASTHSPMAMEDSSHYNREDEVGERIYGEVCANCGS
                     MRLRKNGTCKVCEECGATTGCS"
     gene            complement(4929..5948)
                     /locus_tag="VFD89_05465"
     CDS             complement(4929..5948)
                     /locus_tag="VFD89_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019636912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HZJ57673.1"
                     /translation="MWNPDSYLKALYEGNRPKFKFDVRHMDEYARWRDGLREIFARDL
                     GGFPKPGADLNHTVVEEREYQDYLMQRVVYDEDIDLPIPAYILKPKNVSGKLPAVVAC
                     HGHGYGSKEIVGLNPDGTDNKGEPSYQKNFALELMRRGFIVIVPELLGFGDRRLKEDK
                     DKQPNDSSCHMISTYLLMMGKTMAGHRVYDVMRSIDYLETRDDVDIDRIGCMGISGGG
                     LVCSFAAALDTRIKAAVISGYTNTFIDSVMSIFHCVDNFIPGLVNHAEMPDIIGLIAP
                     RPLLIESGTDDPIFPINATQNAYDKVKEIYKFLDAEDRIDSDFFEGEHEISGVKAYDW
                     LVKWL"
     gene            complement(6015..6836)
                     /locus_tag="VFD89_05470"
     CDS             complement(6015..6836)
                     /locus_tag="VFD89_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZJ57674.1"
                     /translation="MKKKYFTDHVLLPNILHIEDPLGVFSTLIIGQDKALLFDTAFGI
                     GNLKEHVSELTDLPLTVVNSHGHVDHLCGNYHFDQVFIHGKDIPVAESHTSAVMRRGT
                     IAQAKSKGILPDGFDEEAYLATGPGNLALVDEGHIFDLGGITVEVIAVPGHTKGSIGL
                     LLKEEKLILLGDAANPFLFLFLPESTDVAGYIETLNKINGIDFDRFIISHNKDILPKE
                     KINVYIECATNIDIDKSKPFHFPPFKDVKALMYSHGDRRRDDPDFAAIIYTADRL"
     gene            complement(6889..7911)
                     /locus_tag="VFD89_05475"
     CDS             complement(6889..7911)
                     /locus_tag="VFD89_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ57675.1"
                     /translation="MLRVGLIGIGFMGRGHLDIYMQLAEEGHPVELVAICDIDEDKFK
                     GKFLPGNIDVGNLKYDFTKYNLYTDIDEMLEKEELDYVDIALPTYLHAEAAVKALNKG
                     IHVLCEKPMALNTDECQSMIDAAETNGKKLMIAQCLRFWPEYEYLKKCVDSGEYGKVV
                     SGYFFRGGATPMWSYKNWLLNREKSGGVLLDQHVHDVDTINWLFGTPKKLSTIGRNVF
                     PESSYDAVSTNYIYDDMVINAQDDWTINSDDFGFKMSFRVNFEKGALIFEKGKLTVYP
                     HGEKSFVPEMEEGSGYYREIRYFIDALINDTPIETADPYSTMETIRIANAEEESADKQ
                     GELVRF"
     gene            complement(8128..8964)
                     /locus_tag="VFD89_05480"
     CDS             complement(8128..8964)
                     /locus_tag="VFD89_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZJ57676.1"
                     /translation="MKKGINIWSFRGGLSAGEYIRMAKDAGYDGIEFGLDETGIVALD
                     SSDGDIKELKRLADGEGMETPSLASGLYWKYPFTSSDRNIREKAKSIVRRQLDLAALL
                     GADTILVVPGLVGADFIPDGEVTEYDVAYERALDAFMELKGYAEQVKVNIGLENVWNK
                     FLLSPLEMRDFVDKIDSPYVGVYFDVGNVVSMGYPEHWIKILGNRIKKVHFKDFRISV
                     GNINGFVDLLSGDVNYPAVMEQFKNIGYDDYVIAEMGAYRNYPDQTIYTTSIAMDRIL
                     GL"
     gene            complement(9232..9765)
                     /locus_tag="VFD89_05485"
     CDS             complement(9232..9765)
                     /locus_tag="VFD89_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZJ57677.1"
                     /translation="MSYRERTGAEPDTRAETESKIASKTVAYSRTVMSRIMLPSEANP
                     AGNVHGGEIMKFMDTTAFVTAVKHCRTNVVTARVDELEFYLPILVGQLVTCKTQLVFV
                     GKSSMEIAVRVQVEDLKVEEPAKVALTAYFTMVALDDNYKPTEVPGLKLKTPEEEALF
                     KERQEIYLANKHKRKGK"
BASE COUNT         2590 a         2528 c         2047 g         2882 t
ORIGIN      
        1 caattatcat gccggcaaat ttagttgacc tattgtttat cctagataaa caactccaac
       61 tatgaaagct atcagttacc gataagttta gtttacgtat tgtttatcta taaatagtct
      121 cttggatatt ataccctcaa acaattcgac gaaacaagcc ccccttttaa tcggagcctt
      181 tctctgatat aatgggtatg taaggattca tggtccctcc tgtgggatat atggatcttg
      241 aaggtatttt ttatatttta gggaggtatt atatgaagta tcgcaagttt ggagatcttg
      301 gttttgatgt atccgtgttt ggagtaggat gtatgagatt gcctcttaag gtacagccgg
      361 acggtaccac tgaccgcacg aagatagacg aagaagaggc cattaagatg attcgtcatg
      421 ctattgacag tggggttaac tacatagaca cggcttatcc gtatcatggc gggaacagcg
      481 aacccctggt agccaaggct ttaaaggacg gatacaggga taaggtgaag atagcaacca
      541 agctgcctgt atggcaaacc gactcctatg aagattttga aaggatattg gacgagcaat
      601 tggccaagtt ggaagtggag accatagatt tttatcttct tcactcactt tcgaagacaa
      661 gttgggagaa cgccaaggca agaggcgtat tggattttct cgataaggca gtagcgtcgg
      721 gcaagataaa atatcccggt ttttccttcc atgacgaact acctgtcttt aaggatatcg
      781 tagattccta tgattggaag atgtgccaga tccagctcaa tatcttggat gagaactacc
      841 aagcaggggt tgagggaatg cgatatgcgg catccaagaa catccccgta gttatcatgg
      901 aacctctgaa aggcggaaga ctggcccata gtgtacccca ggatatagtt gatctgtgga
      961 acgaagctgg gacaaagcgt tcacctgtag agtgggcgtt tagatggctc tataatttcc
     1021 ccgaaattgt tgtaattcta agcggtgtca gcaccatgga acaattaaag gacaatattg
     1081 aaatattcag taaggccgct cccaattcca tgtccaacga ggagctagag ctggtcaaaa
     1141 gggtcaagga tcaatatgac agcaagatca aagttgggtg taccgcttgc gattattgca
     1201 tgccctgtcc ttcgggagtg gatatacccg gcatatttgc cctttataat gactattcca
     1261 tctacggagg ggcggaggaa ttcaaaaacc gctatcagga atttatagag gacggcaagg
     1321 acgcctctaa ctgtattgaa tgcggacagt gcgaatccga atgcccgcag catatatcca
     1381 taattgaaaa gatacaggag gcccatgagg tcttgacggc gtaacagaat gcgaagcgga
     1441 tctaaactat gaaactgctc caggaatatg aagttatatg cctacaatag ctatcatgca
     1501 tactacgaac aaacatcccc ggacatttcc ggggatgttt tgtttgctat gctaatttag
     1561 cttatgaaca gcccgtagta gcgccacatt cctcacagac cttgcaggtg ccgttctttc
     1621 taagcctcat gctgccacaa ttggcacaaa cctcgccata gatacgctcg cccacttcgt
     1681 cttcacggtt atagtgactg ctatcctcca tggccattgg cgaatgggta gaagcggtta
     1741 ccagatccct agctgacagt atagtcccat ccattccggg ttttgcctcg caccttgaga
     1801 agttacccag ctctatatcg atgatcttgc ttaccaaatc cgatatggaa gaggcgtgct
     1861 ttatataggg atggcggctg acaaagcctg acggctcata ctgctgcccc ctgagcattc
     1921 tggcaatctc tttcggggga atattgtatt ggagcatgtt ggatattgcc ttagacagtg
     1981 aggccaatag cccctttacc accgtaccct ccttgtccgt ggatataaac agctctgcta
     2041 tcccgccttc cggataaaag ttaacggtca catagagttc aatatcacct atcttggcgc
     2101 tatgggtgaa ccctgctctc cttcctgtgg cctttcttcg gacggctgca ccctgtctcc
     2161 cggcctttgc tatatccaga agctctccgt aggacatatc ctccagcctt cttcctgccc
     2221 cattatctac ggaggatgtc aggggctgac tggctttaga gccgtcccta tatatggcta
     2281 tggccttggt accggtagtg taggccagca aatggattct ctctatgtct tccttggaag
     2341 cgctgccggg cagatttacc gtcttggaca cggaccctga aatcagcggc gttacggccg
     2401 ataccatcag aacgtgacct tcgggcgaaa tgacccgatt cccggtccca cacttaccag
     2461 cggtatcaaa tatggaataa tgttggggtt ttagatatgg tgcgccctct atcttgccgt
     2521 ctgcaataaa atcgttgtca tcctttctca ggatatagcc cagaataccg cctatctgtt
     2581 cctccgagta gcccaacctc tctagggcag tctcaatcac cggatttacc atgaccatgg
     2641 aagccccacc ggccagcttt ttatatatta catgggcata aaatggctca atggatgtgg
     2701 cgccgcaatc catggcaaag gatatggtgc cggtaggcgc aaccaccgtg acctgggcat
     2761 ttctgtagcc gtttttctcg ccgctgtcat atgccatatc ccatacccta attaattcct
     2821 caccgatatt atcccacccc agacccttta aggtttcatg gtctatttcc atgggcttat
     2881 agctgagatc ctcataatca tcgtccctgg ccccggccac ccggccgtga tttcttataa
     2941 cccttagcat atgctgcgaa tttatatcat atttttcaaa aggagataat ttctgtgcca
     3001 tcagggacga aacatagtag gagtaccccg tcattatgcc catgatagat gcggccatgg
     3061 ttctggcctc gtgggaatca taggcgtatc cgcacgacat aatcagggaa gccaagttcg
     3121 cgggacccag ccccgttgtc ctgaacatat gggttttcct cgcgatatgc tttgtaggaa
     3181 attgtcccca gtggatagag gcctcaagaa ccagctgcac caaagctacg gtatgcataa
     3241 aatcctctac cctaaactcc cctgtctctg tatctaaaaa ctgataaaga tttatggatg
     3301 ccagattaca ggaggtgtca tccaaaaagg catattcgct gcaggggttg gtcgcattta
     3361 tctggttatg cttcgcccag gtttcaccgt cttccccggc aggacaggtg tgccattggt
     3421 taaacctacc gtgaaattga agcccggggt cggcacaata atatgcggat tcgtttattc
     3481 cgtcccaaag ctcctttacc ttaagctcct tgtttatgct ctggtccaca cgccctttta
     3541 aggttataac ggcttcgggg tcctcatcca gattcataat ccttttcatg gtctcgtcat
     3601 agagcctaac ggaattattg ccgttttggc cggagaccgt gttataggct tcgccgttaa
     3661 agtctatatc ataacccatc ttgcccaggg cacgaacttt atcctcctca agccttttcc
     3721 agttgacaaa gtccataata tccggatggt catcgtccac tataaccatc ttggcggccc
     3781 tgcgggtcgt tcctccggat ttaatggccc cggcattcct gtcaaagccc tttaagaagc
     3841 tcaatagccc ggatgaaaca ccgcccccgg acaacccctc gccctccgcc cttaaggtag
     3901 agaagttggt gccggtgccg gaacccccct taaagagctt ggtctcggtg acgtattgtt
     3961 cggatatgga tctgtccccc ataagcttat cctcgatgga gataataaag caggcggatg
     4021 cctgggttct agaataaagg tctctgcttt ctacaacttc cccactgtcc gtatcatagt
     4081 agaacaagcc gttaggctcg ccttctatac cataggcgtg ttttaacccg gtattaaacc
     4141 attggggtga attaggcgca aaggattggg ataatagcaa gtataccagt tcgtcatata
     4201 ggacttcttt ttgatattca ctatcaacta gtccctcatc aactaaagct ccgatccaaa
     4261 aagataccat ccggtgtacc acctgccgca tactgctctc gtagcctagg tggtccccca
     4321 gccctgcttt tctaaaataa tggctggcaa tgatatcgca ggcgttctga gaataatgga
     4381 cagggaattc caaatctctc atatcattaa taatttcatt agtccgataa tcccttaata
     4441 taacgtctac ccttttccat ttaaaaagat catagaccgt ccgatcctta tgggcctcca
     4501 gttctttggt aaaccgcctt actattaggt catcaatggt tttcatagcc ttccccctga
     4561 ataatattgt gatctagata tgttacgaaa aagacctctc attgtaaaat cggctaaatt
     4621 atccgatttt acgttaaaat aatgtatttt tcgtaacata cgaattaagg ataatgtttt
     4681 acgacacctg tgtgccaatg tagaatgttt gtcgtaaaac atatagatgc caatgaaata
     4741 atccaataat cctatgtatg ggtcaataat acccccaata gggggatctt aacagaataa
     4801 aaaagtaaag cacattacac attatatggt gtgctttaca attatactac actatttcta
     4861 gtatgtaaat gctaaaatgg attatatttc cccctaattc gtcatagtct ggctgtcgga
     4921 tttaacccct ataaccactt taccagccag tcgtatgcct ttaccccgga tatctcatgc
     4981 tcaccttcaa aaaagtcgga atctatccta tcctcggcat cgagaaattt atatatttcc
     5041 ttgaccttgt cataggcatt ttgggttgca ttaataggaa aaatgggatc atctgtcccg
     5101 gattctatga gtaagggcct gggcgctatg agccctatga tgtccggcat ctcggcgtgg
     5161 ttaaccaaac cagggatgaa attatccaca caatggaata tggacatgac actgtctata
     5221 aaggtgttgg tatatccgct gattaccgcc gcctttatcc tggtatccaa agccgcagca
     5281 aagctgcata caaggccgcc gcccgatatg cccatgcatc caatcctatc tatgtccaca
     5341 tcatctctgg tctctaggta gtcaatgctt cgcataacat catagaccct atgtcccgcc
     5401 atggtcttac ccatcatcag cagataggtg gatatcatat ggcaagaact gtcgttgggc
     5461 tgtttatcct tgtcttcttt aagccgccta tccccaaacc ctaagagttc cggtacgatt
     5521 actataaatc cccttctcat aagctctaag gcaaagtttt tttggtagct gggctcacct
     5581 ttattgtccg ttccgtcggg atttaagcct acaatctcct tgctcccata gccgtgccca
     5641 tggcaggcta cgacggcagg cagtttcccc gatacgtttt tgggttttaa tatatacgca
     5701 ggtatgggaa ggtctatatc ctcatcatat accacccgct gcataagata atcctggtat
     5761 tcccgctctt caactacggt atgattgagg tcggcccccg gttttggaaa tccccccaaa
     5821 tcccgtgcga atatctccct cagcccgtcc ctccatcttg catactcgtc catatgcctt
     5881 acatcgaatt taaacttcgg tctgttcccc tcgtataaag cctttaaata gctatcagga
     5941 ttccacatgc tgccccctcc tttcaattac aatattctac attccatcca acaattcctt
     6001 cccggcggaa ctaatcacag cctgtcggcg gtatatatta ttgccgcaaa gtccggatca
     6061 tcccttcgcc tgtccccatg ggagtacata agggccttaa catccttaaa gggcggaaaa
     6121 tgaaagggtt tgctcttatc tatatcgata tttgttgcgc attcgatata gacgtttatt
     6181 ttctccttgg gcaggatatc tttattatgg gatataatga agcgatcaaa gtcaatgcca
     6241 ttaattttat tgagggtctc aatatagccg gctacatcgg tggattcggg caaaaacagg
     6301 aacaaaaatg ggtttgcggc gtcccccagt agaataagtt tctcctcttt gagcaataaa
     6361 cctatggaac ctttggtgtg acccggcacc gctataacct ccaccgtaat gccgcctaaa
     6421 tcgaaaatat gcccctcatc caccaaagca aggttgcccg ggcccgtagc caggtatgct
     6481 tcctcatcaa aaccatcggg caatataccc ttggatttag cctgggctat tgttccccgc
     6541 ctcataaccg cagaggtatg ggactccgct accggaatgt ccttcccatg gataaacacc
     6601 tgatcaaaat gatagttacc gcatagatgg tctacatggc catggctgtt cactaccgtc
     6661 aggggcagat ccgtcaattc cgacacgtgt tcttttaagt tgcctattcc aaatgcggta
     6721 tcgaataata aagccttatc ttgcccgatg attaaggtac tgaatacccc cagcggatct
     6781 tcaatgtgca atatattggg caatagtaca tgatctgtaa aatacttttt cttcatatgg
     6841 tatcatcctc cctggttaat ctattatgcc gattgcatag ccgaagtttt agaaccgcac
     6901 cagctcacct tgcttatccg cagattcttc ctcggcattg gctatcctta tggtctccat
     6961 agtgctgtac ggatcggcag tctctatggg ggtatcgtta ataagggcgt ctataaaata
     7021 tcttatctcc ctgtagtatc ccgatccctc ttccatctca ggcacaaagg atttttctcc
     7081 atgggggtag accgtcaatt tgcccttttc aaatatgagg gcaccctttt caaagtttac
     7141 cctaaagctc atcttaaagc caaaatcatc gctgttaata gtccaatcgt cttgggcgtt
     7201 tataaccata tcatcgtata tatagttggt ggaaaccgca tcatagctgc tttccgggaa
     7261 cacattcctg cctatagtcg atagtttctt cggcgttcca aaaagccagt tgatggtgtc
     7321 cacatcgtgg acatgctggt ctaggagtac cccgccgctc ttttccctat tgaggagcca
     7381 gtttttatag gaccacatgg gggtagcccc gcccctgaaa aagtatccgc tgactacctt
     7441 tccgtactcg ccgctgtcta cgcatttttt caggtactca tattccggcc aaaatctcag
     7501 gcattgggca atcatcagct tctttccgtt ggtttctgcc gcatctatca tggattggca
     7561 ttcgtcggtg ttaagggcca tgggcttttc gcacaagaca tggataccct tattgagggc
     7621 ttttacagcc gcttctgcat gaagatatgt aggcagggca atgtcaacat agtccagctc
     7681 ttccttctcc agcatttcat caatatccgt atagaggtta tacttggtaa agtcatactt
     7741 taggttgcca acatcgatat tcccgggcaa aaacttaccc ttaaatttgt cctcgtctat
     7801 gtcgcatatc gccaccaact ctacgggatg tccctcttct gccaactgca tatagatgtc
     7861 aaggtgaccc cttcccatga atcctattcc gattaatcct actcttaaca taaaatctac
     7921 tcctcctttt gccttaaatt aaccagtggt tccagttata tacaaacaac cctgactaca
     7981 aaatttatca ttataaaatc cagagcccca ttgcatctat catgcagata aatttagttg
     8041 acctattgtt tgtctatatt taccttatta tcttacgtgg ataatgagct gttgaaaagc
     8101 ggttgactta atcaaccccc taacaagtta tagccccaaa atcctatcca tagctatgga
     8161 agtcgtatag atagtttgat ccggataatt tctgtaagct cccatctccg ctatcacata
     8221 atcatcgtat ccgatattct taaattgctc cattaccgca ggataattta catcgccgga
     8281 caacagatcc acaaatccgt taatgttgcc cacactgatt ctaaaatcct taaagtgcac
     8341 ctttttgatc ctgtttccca atatcttgat ccaatgttcc gggtaaccca tgcttacgac
     8401 atttcccaca tcgaagtaga ccccaacata tggactgtca atcttatcta caaaatccct
     8461 catctccagt ggggacaaca ggaatttgtt ccagacgttc tccaacccta tatttacctt
     8521 tacctgctct gcatacccct ttagctccat aaacgcatcc aaagcccgtt cataggcaac
     8581 gtcgtattcc gttacctctc catccggaat aaaatcagcg cctacgagac ccggcactac
     8641 caaaatagtg tcggcgccta aaagagccgc cagatccaac tgtcttcgga ctatggactt
     8701 ggccttttct ctaatattcc tgtcggaact ggtaaaaggg tatttccaat acaatccgct
     8761 ggccagactg ggagtctcca taccctcccc gtctgcaagt ctttttagct ccttgatatc
     8821 cccgtcgcta ctgtcaagcg ccactatacc ggtttcatcg agcccaaatt ctatgccgtc
     8881 ataccctgca tcctttgcca tccggatata ctccccggca gaaagtccgc ccctaaaaga
     8941 ccagatgttt atcccctttt tcacctgtac caccccatcc ttacttaaat taacctattg
     9001 gcattagatt actatatctg caatgactat tgaacgccca gtcataacgt aaaagtaact
     9061 gtatagttga agtttcaaaa aaatgaatca atattattgc agcagaagtt attaataatg
     9121 tattttttga aacttcctta cttccgacat tgataatata tatcagaccg gcacacatat
     9181 tagattaatt cgttatgccg atcaaaggca tgcattatgg atatcgggta tttatttacc
     9241 cttccttttg tgcttgttgg ctaagtatat ctcctgtctc tccttaaaga gggcctcctc
     9301 ttccggggtc ttcagcttca agcctgggac ctcggtaggt ttgtagttat catctaaggc
     9361 caccatggta aagtaggcgg tgagggcgac ctttgcaggt tcttcaacct tcagatcctc
     9421 tacctggacc ctgaccgcta tttccataga actcttccct acaaagacca gctgggtttt
     9481 gcaagttacc agttgtccaa ccagtatcgg caagtaaaat tccaattcgt ccaccctggc
     9541 cgtcaccaca ttggttcggc aatgctttac agcggtaaca aaggctgtgg tgtccataaa
     9601 tttcataatc tcgccgccat ggacatttcc tgcggggttg gcctcgctag ggagcatgat
     9661 ccttgacatg acggtacggg aatatgctac ggtctttgat gcaattttgg attcggtttc
     9721 tgcccgggtg tcgggctctg cccctgttct ctctctatat gacatttgta tcctccgtat
     9781 aagttatatt ggtgtacggg agaccatatc gggcctcctt ggtgccgtat ctatatacaa
     9841 agccgggctt tgtcgtctcc ggataatgta tatggcctac cggccgagtg tcggtaacgg
     9901 gcgttatcat tttaacctgt tatagataaa caactccaac tacaaaattt atcgtcatat
     9961 aattgcatag aaccattgca attatcatgc caacaaattc agctgatcta ctgattatct
    10021 atataaacct caacatatga attatac
//
LOCUS       DASRNX010000136        24325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_398500,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000136 DASRNX010000000
VERSION     DASRNX010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 24325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24325
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398500"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..870
                     /locus_tag="VFD89_05490"
     CDS             142..870
                     /locus_tag="VFD89_05490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZJ57678.1"
                     /translation="MQRKTPGKLPLDLPVPRVRYRNPAYRGKADMGEYDTDKTAPLFR
                     KIFICAVIIVLVLVLKYMDTDFSKNVVGYIRDGITNHIDIDEALGKLKFVNNYLPDNI
                     AVFGEQPVTAGGDAGMPDGLAPFFAIPAEGKVIERFSSNNRGINISGSKNDNVYAVAE
                     GLVLDIGEDEEGGKYVKLDHGNNVTTLYKGCSLIDLSTGERTTKGQSIGKMGGTALEG
                     YTLYFEAQVDNRPVDPLALIELGK"
     gene            895..1779
                     /locus_tag="VFD89_05495"
     CDS             895..1779
                     /locus_tag="VFD89_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HZJ57679.1"
                     /translation="MRIGSIFDVDINISITLVVLLVIAVFLGNGAEIAVMFFVFMAHE
                     TAHVLVARMLKMKVGEIELLPFGGAVRIESVFELNPASEIYIALAGPGMNIMLLLVYS
                     ALQTLGLVSVKENDIFVQANLILAGFNLLPALPLDGGRVLRAILAREIGLKRATNIAS
                     YGGMILALFLAMTGIYALSIKVFNPNFFLLSGFLAYSAHKEKRTASYIFIRDITFKRN
                     MLSKEGLLTTKEIVVLYDLPLKAVINSFVPHRYHFVKVVDHSLRVWGYLDETEILGGV
                     LQYGVNARIGRLLRREQL"
     gene            1904..3712
                     /locus_tag="VFD89_05500"
     CDS             1904..3712
                     /locus_tag="VFD89_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HZJ57680.1"
                     /translation="MGQVEKPVRYMGNEYNMVVKDVDKVDIRFAFVFPDVYEVGMSHM
                     GMKILYHLINQRDDAYCERVFAPWVDMEQRMRENNIPLFALETKTPLADFDIIGFTLQ
                     YEMSYTNVLNMLDLGGIPLTWEERGENYPLIIAGGPCAYNVEPMADFFDLVVLGDGEE
                     VIQEILDLYLVCRGQGLSKSEFLYKAAGIKGIYVPRFYGVEYKEDGRVSVVSPIETGI
                     PDIIQKRVVRDLDNTYFPDTTIVPYMDVVHDRITLEIFRGCTRGCRFCQAGMIYRPVR
                     ERSPEKLLELAEKLVENTGYEEISLSSLSTGDYSRLEDLVTKLIAEFKDKRVGLSLPS
                     LRLDSFTGEVADEVKKVRKTGLTFAPEAGSQRMRDIINKGITEQDLMLSVEDAFKSGW
                     TRVKLYFMLGLPEETIEDLKGIGDLAHKIRGCYFDMGRGGRKALKITISISSFVPKAF
                     TPFQWVSQNTMEELAGKQSLLQKELRGRNIQYNWNDPKVSFIEAVLARGDRRLGRVLR
                     KAWEKGCVFDGWTEHFNFDLWMEAFKETDIDPHFYAGRTRQADEVFPWDHIDVGVTKK
                     YLWREYERALKAQLTDDCRVACTGCGVSELGVGICP"
     gene            3703..4404
                     /locus_tag="VFD89_05505"
     CDS             3703..4404
                     /locus_tag="VFD89_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HZJ57681.1"
                     /translation="MSIRLIVRYTKGERVKYISHLDFVRLVQRAIRRADIPVAYSQGF
                     NPHPKLSFASALAVGTTSQGEYLDIILEKDVDPELLCKRMNEKLPAGISFEEGRVADE
                     KLPSLMSLIERAGYIITMPEGIPWENDIPSTIDRFLMQQEILITRTTRRGRRQIDIRP
                     MIHEMDVFESHRGTAVKAMVSAGSKANLRPELLMDALLNFAGTSMGPDLFFHVHRLDL
                     YLLQEDKWVTPIGME"
     gene            4417..5949
                     /locus_tag="VFD89_05510"
     CDS             4417..5949
                     /locus_tag="VFD89_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZJ57682.1"
                     /translation="MSKRIIVDVQYDQTRVAILEEGELVELYMEGDDNHTIVGNVYRG
                     KVENVLPGMQAAFVDIGLEKNAFLYVGDINTDKSVFEFSGKQSTSVNDARALSIRDII
                     SKGEELTVQVLKEPIGTKGARVTTQITLPGRYLVLMPTVNYVGVSRRIEDEAERARLK
                     EIAERLKPHGMGMIVRTAARGMDRDDFISDIDFLCRLWARIQSKEWRGKVPRALYEDE
                     DIIYRTIRDFYAPDIEKIVINDREEYDKTLEFADVLFPGLRNRMELFDGSIPVFQHFD
                     IEYKIEKAIQKKVWLRNGGYLIIDPTEALTVIDVNTGKFVGSRNLEDTVLRTNLEAAQ
                     EIAHQIRLRDIGGIIIIDFIDMGKEDNKNKVLEVLESSLKKDRTKTNVLGFTGLGLVE
                     MTRKKVRRRLSSKLLRPCPYCHGTGRVYSETMVVARIEKELEGEVKDSDTWGVVVEVH
                     PSVARTWTEDGGKNIDILEKTLNCRIYLRDNHSLHIEDSIIHPLADQREAEKYFLEHK
                     ES"
     gene            6070..7290
                     /locus_tag="VFD89_05515"
     CDS             6070..7290
                     /locus_tag="VFD89_05515"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017056948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZJ57683.1"
                     /translation="MKIIKKSRKLDNVCYDIRGPILDESKRLEEEGYNIIKLNIGNPA
                     PFGLKAPDEIIVDVIMNLKNAQGYSDSKGIFAARKAVMQYCQQKDIENVGIEDIYIGN
                     GVSELIMIAMQGLLDNGDEILIPAPDYPLWSAAVNLAGGTPVHYICDEESDWYPDLED
                     IRKKVTPDTKGIVVINPNNPTGSVYPIDILKGIIDIAREHDLIVFSDEIYDKILYDGV
                     THTSTAALADDILFITMNGLSKSHRIAGFRAGWMIISGDKSGAKDYIEGLNMLASMRL
                     CSNVPAQYSIQTALGGYQSIQELILPGGRLKEQRDYAWERLNGIPGISCVKPQGAFYM
                     FPKVDVKKFSIVDDEKFILDLLLQEKVLLVQGTGFNWPGHDHFRMVFLPNLTDLKIAL
                     DRFEYFLSDYSQKS"
     gene            7599..8102
                     /locus_tag="VFD89_05520"
     CDS             7599..8102
                     /locus_tag="VFD89_05520"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZJ57684.1"
                     /translation="MSKIRNTSGILEREVSKLRRYEFFNRLEGIIAEIGTAILANIDE
                     DGTPRMRWMTPATLKGRPGFIYCVGAGNSKKVKGLRINPKAEWMIQSKNLDCIINIKG
                     TVNILDNPSIRTEILHTIGPRLGMFWKTNLEDTEFVVLETVMDEAIFFEPMLCKSKRV
                     SFREGDE"
     gene            8099..9082
                     /gene="pdhA"
                     /locus_tag="VFD89_05525"
     CDS             8099..9082
                     /gene="pdhA"
                     /locus_tag="VFD89_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HZJ57685.1"
                     /translation="MSRTFEITKYNEDFLIDLLENMILIRRFEEKAGQMYGLQKIGGF
                     CHLYIGQEAVAVGSIAALDLAKDYVLTTYRDHGHALACGMEPGVLMAELYGKKTGCSK
                     GKGGSMHLFDVENHMYGGHGIVGSHIPLATGVALKIKYKEDDGVVACYFGDGAIHQGS
                     FHESLNMARIWDLPIVFICENNQYGMGTNYERISSVHDFYKMGDSYGIPGKQVDGMNV
                     LEVYEEMLQAAKTARESGLPSFLEIKTYRYRGHSMSDPAKYRTREELDEYRRQDPVLL
                     FKDALIEYNVITEEDYREMDNRYKEVANRATEFAENSEEPGLETLYEDILA"
     gene            9100..10098
                     /locus_tag="VFD89_05530"
     CDS             9100..10098
                     /locus_tag="VFD89_05530"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HZJ57686.1"
                     /translation="MEEDVRMPIITYRDALRDAMDEEMARDENVILLGEEVAEYNGAY
                     KVSQGLWDKYGSKRVIDTPITEEGFTGVGIGAAMAGLRPIVEWMTLNFSILALDQVIN
                     NAAKMRYMSGGQFKVPIVFRGPNGPAEYLAAQHSQALQTLYVHIPGLKVVAPATPYDA
                     KGLLKSAIRDDNPVIFLEGELMYQWKGEVPEGEYLVPIGKADIKRKGQDVTIITYSKP
                     LRIVEEAAMELANQGIEAEIVDLRSLRPIDEETIYRSVKKTNRCVVVDESWPMVSVAS
                     HVGWMVSRNCFDDLDAPVELVTGEDVPMPYNHRLELAAQPSAGKVVDAVKKVLYLS"
     gene            10118..11353
                     /locus_tag="VFD89_05535"
     CDS             10118..11353
                     /locus_tag="VFD89_05535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZJ57687.1"
                     /translation="MAEKVLMIALSPTMETGLISKWEKKEGERVESGDLLCQVETDKA
                     TMDYESIQEGTLLKILVREGEQAGIGSPIAIIGETGEDIAEILAEIEAETKDSPSDGT
                     EEVKEEVAATPAIEEAEEYTGDRIIASPLARRMAEHQGIDLTYIQGSGPRGRIIKRDI
                     LEAASQPQMPGTPTPKSIPRILPTAADEVVPVSIKRKIIADRLAESKYSAPHYYLKLR
                     VASDGFMKARKMLNDTLSERISVNAFIIKFVAEAIKRHPVINSTWQGETILGHGSIDI
                     GLAVAQTDGLITPVVRDCGNKGIIEIDKELRDLIHKALNNQLTPPEYSGATFTVSNLG
                     NYGIEEFTAIINPPGSAILAIGKMGKEPVVVDGDRIEVQSLMALTLSCDHRVIDGAVG
                     AAFLKDLKDIMENPIRVLY"
     gene            11538..12932
                     /gene="lpdA"
                     /locus_tag="VFD89_05540"
     CDS             11538..12932
                     /gene="lpdA"
                     /locus_tag="VFD89_05540"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZJ57688.1"
                     /translation="MDTKFDYDILIIGSGPGGYVAAIRASQLGLKTAVIERDKPGGVC
                     LNVGCIPSKALINQAGIYQNRAQLMNMGIKLDESGFDYGKVFKKSRKAANTLARGVEY
                     LLKKNGVELIMGEGFIESPNRVAVGDRSLLAKNIIIATGSSPRQIPGFPVDEVKVLSS
                     TGALMLEELPKDIVILGAGAIGMEFAYILNSFGVEVHVVEMMDSILPLEDKETVEVLR
                     RTLRKTGIKFYTSTKALSMEEKDNGRILVALEDAKGNVTEREAEKILVAVGRKPNTHG
                     IGLENLGIETVRDFVPVGDYYQTKVQGVFAIGDVVDSPLLAHVASKEGEIAAEFIAGH
                     KPEPRIDLNAVPSAVYCEPQIASFGFTEDRAKNEDIPYEKAVFPYRGAGKSVAIERSE
                     GMVKILYDPVTHELLGGHIVGAEATELIHELLLAKWGEMLPEDIAGMIHAHPTLAEAV
                     METARSAEGWAIHM"
     gene            13577..13843
                     /locus_tag="VFD89_05545"
     CDS             13577..13843
                     /locus_tag="VFD89_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZJ57689.1"
                     /translation="MIEREFAIKDDMGLHAGSAAKFTRIAGRYKSEIRVIKGNKTGNG
                     KSLLSILALGIFGDTKFVVKIVGPDKIEAMYDLTRLIENDFILD"
     gene            13882..14931
                     /gene="dinB"
                     /locus_tag="VFD89_05550"
     CDS             13882..14931
                     /gene="dinB"
                     /locus_tag="VFD89_05550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZJ57690.1"
                     /translation="MTELGIIHVDMDAFYASVEQRDHPEFRNKPVVVGGDPKGRGVVS
                     TASYEARRFGVHSAMPCRQAAELCPNIIFLPVDMEKYKRVSKKIHSIFRIYTPTIEPI
                     SIDEAFLQVDGGDPVDIGKEIKGDIYKRLHLTASVGVSYNKFIAKMASDMEKPDGFTV
                     ITREEARKILPGLPIRKIWGVGEKTEKDLNNIGIFTVNDLLQYDHEFLLRSWGRRAYE
                     LIQLCRGIDHSMVQIDQETKSMGEETTLEGDTRDLDVLKGILGDFSMELGNRMAGRGI
                     KCRTITIKVKYNDFKSITRSITLAVATDSSHIILQHSRDMLANRVSLSKPVRLIGVQV
                     SNLVYPDEPVQISFI"
     gene            15021..15284
                     /locus_tag="VFD89_05555"
     CDS             15021..15284
                     /locus_tag="VFD89_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57691.1"
                     /translation="MEAGLMIKIYTQEGNKKLNKQYLGTKRAISQIAYEKMQEYMRTA
                     DVGLGSDERNILEILIANSMMQSFSLGYGIGKVEGLTNKQIHL"
     gene            15475..17172
                     /locus_tag="VFD89_05560"
     CDS             15475..17172
                     /locus_tag="VFD89_05560"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HZJ57692.1"
                     /translation="MWGFNGKLKRSMGGYTRLGILVIILGILIATILLFGAAKFTEDR
                     SFFILSPNTFLALHILLEFMAIVMAFCIFAITYYTSGETKSLSMIIIACVFLATAPLD
                     ALHTFTYKGMPLFIVTPSANTATLLWIASRFIMSTGLLLASLIPEDRGTKGDQSPYVA
                     ITIGLIGLLMGLVLYRPGLFPPMYTEGSGLTPLKIGLEYLITGMLLVTAMVYYGKFSR
                     SHGDREYFLLAIGFFISGLGELAFTLYSDVYDTYNLLGHMYKFLARYLLFRSLFVINI
                     KKPYRNLWEAEKKLNNYVDDLEESVTQRTMEIRRANEKLLKNLKDAKHIQRALMTTEF
                     PKISGMEFAAKYLPCEQVGGDFYNIFKLDEHNVGIVIGDVAGHGVSAAMVNVFINQNM
                     RFRVDYDEQRYRILTPRGVLMNLYHVYNNMSFPDEMYVVLFYGIYNTVTGKLSYSSAG
                     MNTYPLILSAKGEISHIKLDGFPICKFGKFFRPDYETKTLQLSSGDTLIFYSDGLGEI
                     DRKQPDILSSENIMEYLRGMRHYSAREVCQGLFDAYHALLADNEMLDDVTILVIKTP"
     gene            complement(17213..18202)
                     /locus_tag="VFD89_05565"
     CDS             complement(17213..18202)
                     /locus_tag="VFD89_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57693.1"
                     /translation="MDRYIPLRYSSNINPKINPGNTIDLPKDADKRSGTQKKMPPGFT
                     YAPPAQANPLPIILLILLVSPYIQRARSDSIRLYNSEESQADGSDTVVDILTSIYPYL
                     DPKHQDGINFIVGLAKTKANLQGLIDGTYQTPRISGASKPITSYKERAVGIIKALQPH
                     ISLENQTQINRILHVNNTLEKLVDSIERFKERNSIKAQDSAADKISRAMEMIDILKIL
                     APAEQLQYIDQVSNVLRLVGTIELAQQITPSIQDSSRVMDGAGAVDGKEQGQSFDDSG
                     ATRDATKERDEKVENISKAFKSVLNPDQAKSLELIMNMAQLLSKDSSDKNGAD"
     gene            18393..18611
                     /locus_tag="VFD89_05570"
     CDS             18393..18611
                     /locus_tag="VFD89_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57694.1"
                     /translation="MDINKDSLETMAGLFGNLEGKSEDEMISELARMIRAGQGGITVD
                     KARQMIQTILPMTDSNQKRKLEKLLRIL"
     gene            complement(18630..19787)
                     /locus_tag="VFD89_05575"
     CDS             complement(18630..19787)
                     /locus_tag="VFD89_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZJ57695.1"
                     /translation="MIYMDNAATTWPKPDVVYNAVMKTMKYYGANPGRSSHNMAVEAA
                     NILLYTREMLCQLFNAGDPFRMIFTYNATDSLNLAIKGILNKSDHVITTSMEHNSVIR
                     PLMHLADLGVETTVVPADSEGLIDPDDIKKNIRSNTRLIISTHASNVTGTLMPIEAIG
                     KIAEASGVYFLLDAAQTAGLVPIDVSKLSVDLVAMPGHKGLLGPQGTGVLYVKEGIAL
                     KQIREGGTGSQSESVYQPMFLPDRYESGTLNTPGIAGLGAGIGYILGNSQDRALDHVA
                     NMEKLLVNALWQIRGIKVYGPKDMKHRSGIVSINVWDRDSREIVNILNADYGIAARGS
                     LHCAPLAHETIGTKEQGTVRLSPGEFNTLDEVKACVKALGEISYKIKTRSI"
     gene            20068..20343
                     /locus_tag="VFD89_05580"
     CDS             20068..20343
                     /locus_tag="VFD89_05580"
                     /inference="COORDINATES: protein motif:HMM:NF023251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HZJ57696.1"
                     /translation="MPAVEEFNFISFNSRQHALYFDQLLRNAGYDSQIISTPKGVSLG
                     CGLSLRFSPNLTGAIIDIYRRNQIPIIGFYSIRRMGSYSQLRRIPTY"
     gene            complement(20350..21207)
                     /locus_tag="VFD89_05585"
     CDS             complement(20350..21207)
                     /locus_tag="VFD89_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57697.1"
                     /translation="MGSDQSVHRLIRFDGDRMWDFFSDGRNNICYRTLEGNMGWMGPH
                     LVSENAKNEFGVCVDEHGGLHFIYLSLEGGITYMHYDDKGWSEQKLSEYNVKEYDIRY
                     PAVIALDNRIHIIFAVGDPWEAEIWSLYHYCWDGKLWVHQRISEFIDDWESAPYSYRI
                     YDNSIHLSYREVSGSISKVLHVKYHTDLQKWLALPNNPYAGADIGDNFSPEPSIYEDA
                     LEWINKAQAVYRDHLSIARETDELHRRSMETKRRWELILEELDDFKIKIRKMQNKGPL
                     KRLFGSMLH"
     gene            21640..22272
                     /gene="nth"
                     /locus_tag="VFD89_05590"
     CDS             21640..22272
                     /gene="nth"
                     /locus_tag="VFD89_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZJ57698.1"
                     /translation="MSNKIGDIDKILELLEERYSGAGPELHHKNPFQLLIATILSAQC
                     TDRQVNRVTPNLFKRYPKAEDLAALDPQELEPHIKSCGFYKTKSKNIVATCRLILERF
                     GGEIPRDIETLQTLPGVGRKTANVVASNAFGVDAIAVDTHVFRVSNRLGLAKAGNVLE
                     TEKQLMDNIPKDKWTAAHHWLIHHGRQICNARNPKCDVCFLTPYCPYYND"
     gene            complement(22427..23158)
                     /locus_tag="VFD89_05595"
     CDS             complement(22427..23158)
                     /locus_tag="VFD89_05595"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HZJ57699.1"
                     /translation="MEKIIAFGTDLSKSSADRTNNVITAALAFNGLMVSPGETVSFNE
                     VTGKRTSDKGYGYAPMISNRRFVDVLGGGVSQTSTTLYNTAIRAGLDIIERTRHSLPS
                     SYIDMGLDTTVNLPPPEPTIDLKFRNNRGSPIYIRAYFADKRVYFEIYGEPLPRGQRY
                     EFYSEVYETIPAPAPDIIVDYEARYTEFEGEEYIHTESRNGYKVRVFKQTFQDDELVG
                     EELFDTHYYRPVKGKTYIGKKKATN"
     gene            complement(23179..23847)
                     /locus_tag="VFD89_05600"
     CDS             complement(23179..23847)
                     /locus_tag="VFD89_05600"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HZJ57700.1"
                     /translation="MRTRRKKTTGQRKKPIAIVILFIALILMAGTGLRAYINRILDTD
                     TFYEGITIDGLSMGGLTKQSAEKLLREKNQARLDNLKVGLLHQSDFWTLGYKDMGVSI
                     DISEKTKEAYGIARTGHPLKRFLMVQRLRREGRSFHTKLTYSVDSLQAEVERVAAAID
                     RDTVDATVTFNPDGEKKFNISPEEEGLSLDTSMVISQIREVLDGKTTTDTIILSPNRL
                     TPKV"
BASE COUNT         6911 a         4870 c         6122 g         6422 t
ORIGIN      
        1 atatttacta tgtggatgac ctttaccgtt aaaaataggg gagtaggcgt ccataaaatc
       61 aaccggatgc aggaggtacg cgattagcct tgcagttcgt tgatttttgt agtcaaaata
      121 ttcaagggga gggtaataaa gatgcagaga aaaaccccgg gtaaattacc tttggactta
      181 cctgtaccga gggttaggta tcggaaccct gcctataggg gaaaggccga tatgggagaa
      241 tacgacaccg ataaaactgc acccctattc cgtaaaatat ttatatgcgc tgttatcata
      301 gtattggtgt tggtattgaa atacatggat actgactttt ccaaaaatgt agtcggttat
      361 ataagagacg gcataactaa ccatatagat atagacgagg cgctgggtaa attgaagttc
      421 gtaaacaatt atttgcctga taatatagcg gtatttgggg aacagccggt aacagcgggt
      481 ggtgacgccg gcatgcccga tggcttagct cctttttttg ccattccggc cgagggtaag
      541 gtaattgagc gtttcagctc taataatagg ggaataaata tatcggggag caaaaacgac
      601 aatgtctatg cggtcgcgga gggactagtc ctcgatattg gggaggacga agagggcgga
      661 aagtacgtta aattggatca cggaaataat gtaacgaccc tgtataaggg gtgttcccta
      721 attgatttaa gtacaggcga aagaacaacc aagggccaaa gcattggcaa aatgggcgga
      781 accgcccttg agggatacac cctgtacttt gaggcacagg tggataatcg accggtagat
      841 cccctggcgc tgatagagct gggtaagtaa tccttgggac gcataaagag tataatgaga
      901 atagggtcta tatttgatgt agatatcaat attagtataa ccttggtagt cttgcttgtt
      961 attgctgtat tcttgggaaa cggtgcagaa attgccgtga tgttctttgt ttttatggcc
     1021 catgaaacag cccatgtctt ggtagccagg atgctaaaga tgaaggtggg ggagattgag
     1081 ctgctcccgt ttggcggtgc ggtcaggata gaaagcgttt ttgaactcaa tcccgccagc
     1141 gaaatatata tagcccttgc aggtccggga atgaacatta tgttgctgct ggtatactcc
     1201 gctctccaga ccttgggcct tgtaagcgtg aaggaaaacg atatctttgt acaggcaaac
     1261 ctaattcttg caggctttaa tctgttgccg gcccttccct tagatggggg cagggtatta
     1321 agggctatac ttgcccgaga aataggcctt aaaagggcta ccaatatagc ctcttacggg
     1381 ggtatgatat tggccctgtt tctggccatg accgggattt acgctctgag cataaaggtt
     1441 tttaatccca acttttttct cctgtcggga tttcttgcat actcggccca taaggagaag
     1501 cggacggcat cttatatatt tatcagggat atcaccttta agcgtaatat gctatctaag
     1561 gaaggattgt taacgaccaa ggagatagtg gtattatacg atctgccgtt aaaagcggta
     1621 ataaacagtt tcgtacccca ccgctatcat tttgtaaaag tagtagatca cagcctcagg
     1681 gtctggggat acctagatga gacggaaata ctgggtgggg ttctgcaata tggtgtaaat
     1741 gcccggatcg gcagattgct ccggcgtgag cagctatgac gggactctga attatgataa
     1801 aagcaccatg atacggtggg gaatacccca cattttagga atatacttat gaggggacaa
     1861 atgaggaggc cggatattgg acaaacaatt gctggatagg cttatgggac aagtggaaaa
     1921 gccggtgagg tacatgggaa atgaatacaa tatggtggtt aaggacgtgg acaaggtaga
     1981 tattcgattt gccttcgttt tccccgatgt atacgaggta ggtatgtccc acatggggat
     2041 gaagatcctt taccacctta taaatcaaag ggatgacgcg tattgtgaaa gggtatttgc
     2101 cccctgggtg gatatggaac agaggatgcg ggaaaacaac atacccctgt ttgccttgga
     2161 aaccaagacc ccattggctg atttcgatat tatcggtttt accctgcaat acgagatgag
     2221 ctataccaat gtccttaata tgctggattt ggggggaata cccctgactt gggaagaaag
     2281 gggcgagaat tatcccctga tcattgcggg aggaccctgc gcatacaatg tcgagcctat
     2341 ggcagatttt tttgatctgg ttgtcctggg tgacggcgag gaagtgattc aagagatttt
     2401 ggacctgtat ttagtctgca gggggcaggg actgagtaaa tccgaatttt tatataaagc
     2461 ggcagggata aaggggattt acgtccccag attctatggg gttgaatata aagaagacgg
     2521 cagggtgtct gtggtaagtc ctattgaaac gggaatcccg gatattatcc aaaaacgggt
     2581 agtaagggat cttgataaca cctattttcc ggacaccacg atagtcccct atatggatgt
     2641 agtccatgac aggatcacgc tggagatatt cagaggctgc actaggggat gcagattctg
     2701 ccaagcgggt atgatctaca gacctgtacg ggaacgatcc ccggagaagc tgttggaatt
     2761 ggccgaaaag ctggtagaga atacgggata tgaagaaatt tcgctatctt cgcttagcac
     2821 cggagattat tcccggctgg aggatttggt aacaaaatta atagccgaat ttaaggacaa
     2881 gcgggtaggt ttatccttgc cctcccttag gcttgattct ttcaccggag aagtagccga
     2941 tgaggttaaa aaggtaagaa agaccgggct taccttcgcc cccgaggccg gcagccagcg
     3001 gatgagggat attataaaca agggaataac tgaacaggat cttatgctca gcgtagaaga
     3061 cgcctttaaa tcgggatgga caagggtaaa actttatttt atgctgggtc tcccggaaga
     3121 aaccatagag gatttgaagg gaatcgggga tttggcacac aaaatccggg gttgctattt
     3181 tgatatggga aggggaggca gaaaagcact gaagattacc ataagcatct cgtccttcgt
     3241 tcccaaggcc tttactcctt tccagtgggt atctcagaat accatggagg aactggccgg
     3301 aaagcaaagt cttttgcaga aggaactcag gggcagaaat atccaataca actggaatga
     3361 tcccaaggtc agcttcatcg aggccgtttt ggccagggga gacagaaggc ttggcagggt
     3421 gctcagaaaa gcctgggaga agggttgcgt cttcgacggc tggaccgagc attttaattt
     3481 tgatctatgg atggaagcct ttaaagagac ggatatagac ccccactttt atgccggcag
     3541 aacaagacag gccgacgagg tatttccctg ggaccatatc gatgtcggcg ttacaaaaaa
     3601 atatttatgg agagaatacg agagagccct taaagcacaa ttgaccgatg actgccgggt
     3661 agcctgtacg ggctgcggtg ttagcgagtt gggggtggga atatgtccat aaggctgata
     3721 gtgaggtata ctaagggaga aagggtcaaa tacatttctc acttagattt cgtaaggctg
     3781 gtacaaaggg cgattaggag ggcggatatt cccgtagcct atagtcaggg gtttaatccc
     3841 catcctaaac tgtcatttgc gtcggcctta gccgtgggaa ccaccagcca gggggaatat
     3901 ttggatataa ttttagagaa ggatgtggat cctgaactat tgtgcaagag gatgaacgaa
     3961 aagttacccg ccggaatcag cttcgaggaa ggacgggtag cggatgaaaa actcccatcc
     4021 cttatgagcc tcatagaaag ggcagggtat ataattacga tgcctgaagg gataccatgg
     4081 gagaatgata tcccatctac aatagacaga ttcctgatgc agcaggaaat acttataaca
     4141 aggacaaccc gcagaggcag gcggcagatc gatataaggc cgatgattca tgaaatggat
     4201 gtatttgaaa gccatagagg taccgctgtc aaagccatgg ttagcgcggg gagcaaggct
     4261 aacttaaggc ccgagttatt gatggacgcc ctattgaatt ttgccggaac atcgatgggt
     4321 ccggatttat ttttccatgt acaccgtctg gatctgtatc ttctgcaaga ggataaatgg
     4381 gtcacaccta ttgggatgga atagggggat aaaacattga gcaagaggat catagtagac
     4441 gttcagtatg accagacgcg cgtcgctatt ttagaggaag gcgagctggt ggagttgtat
     4501 atggaaggcg atgataatca caccatagtc ggtaatgtat acaggggtaa ggtagagaac
     4561 gtacttcccg gtatgcaggc cgcctttgta gacataggcc ttgaaaagaa tgctttcctc
     4621 tatgtaggag atatcaatac cgataaatcc gtttttgaat tttccggcaa gcagtctaca
     4681 tcggtgaacg acgccagggc attatccata agggatatca taagcaaagg tgaggaattg
     4741 accgtccaag tgctgaagga acccataggc accaagggcg ccagggtgac cacccagatt
     4801 accttgcccg gaaggtatct ggtcctaatg cccaccgtta attacgtggg cgtatccaga
     4861 aggatagagg acgaggccga aagggcgcga ttaaaggaaa ttgcagaaag actgaaaccc
     4921 catggtatgg gtatgatagt caggacagcg gcccggggca tggaccggga tgattttatt
     4981 tctgatatag actttttatg ccgtttatgg gccaggatcc aaagcaagga atggcggggc
     5041 aaggtaccca gggccttata tgaggatgag gatataatct ataggaccat cagggacttt
     5101 tatgcgccgg acattgaaaa aatcgttata aatgacaggg aagagtatga taagactttg
     5161 gagtttgccg atgtattgtt cccgggatta aggaacagaa tggaattgtt tgatggctca
     5221 attccggtat ttcagcactt cgatatcgaa tataaaatag agaaagctat tcaaaagaag
     5281 gtttggctga gaaacggggg ataccttatt atagatccca cggaggccct gactgtaatt
     5341 gatgttaata cgggcaaatt tgtagggtca agaaacttgg aggataccgt gctaaggacc
     5401 aaccttgagg cagcacagga gatagcccac cagatcaggc ttcgggacat agggggcata
     5461 ataattattg attttattga tatgggcaag gaagacaaca agaataaggt attggaggtt
     5521 ttggagagtt cgcttaaaaa agaccgaact aaaaccaacg ttttgggatt taccgggctg
     5581 gggcttgtgg aaatgactag gaagaaggtc cgccgcaggc tctcatcgaa gctcctcagg
     5641 ccttgcccat actgtcatgg tacgggtagg gtatactctg agaccatggt agttgccagg
     5701 attgaaaagg agttggaagg cgaggtcaaa gactctgata cctggggagt tgttgtagaa
     5761 gttcatccaa gcgttgcaag aacatggacc gaagacggcg gaaagaatat cgacatttta
     5821 gaaaagaccc taaactgcag aatatatctg agggacaatc atagccttca tattgaagat
     5881 agcataatcc atcccttggc cgatcagcga gaggctgaaa agtattttct ggagcataag
     5941 gaatcataga ttatcacggg ggcaccgccc cctctttttt ttcaggccgg gtggttcttg
     6001 tatggataaa ggcgtactga tatagtaaat aacgaaagac aggaaattta acagagaggg
     6061 cgagttaaat tgaagattat aaaaaaatcc agaaagctag ataacgtatg ttacgatata
     6121 aggggaccaa tcttggatga atccaagaga ttagaggaag agggttacaa catcataaag
     6181 ctcaatatag gaaaccccgc accctttggt cttaaagccc ccgatgaaat aattgttgat
     6241 gtaataatga atttgaagaa cgcccaagga tacagcgatt ccaaggggat atttgccgcc
     6301 agaaaggctg tcatgcaata ttgtcaacaa aaggatatag aaaatgtagg gatagaagac
     6361 atatatatcg gaaacggagt aagcgagctc ataatgatcg ccatgcaggg tctgttggat
     6421 aatggggacg agattttaat cccggcaccc gattatcctc tatggtcggc tgccgtcaac
     6481 cttgccgggg gaacccctgt acattatatt tgtgatgaag aatccgattg gtatcccgat
     6541 ctggaggata tccgaaaaaa ggttacccct gacaccaagg ggatagtcgt tatcaacccc
     6601 aacaatccca cgggatccgt ttatcccatt gatatattaa agggaatcat cgatatagcc
     6661 agggagcacg atctaatcgt attttcggat gagatttacg acaagatact gtacgatggg
     6721 gtcacccata cctccactgc ggcgctggcc gatgacatac tctttattac catgaacggc
     6781 ctgtctaagt cccatagaat tgccggattt cgtgccgggt ggatgataat aagcggggat
     6841 aaatccgggg ccaaggatta tatagaaggg cttaacatgc tggcatccat gagactttgc
     6901 agcaatgtac cggcccaata cagtatccag accgcccttg gggggtacca gagcatacaa
     6961 gagctgatcc tgcccggggg caggttaaag gagcaaaggg attatgcgtg ggagcggctg
     7021 aacggcatac cgggaatctc ttgcgttaag ccccaaggcg cgttctatat gttcccaaaa
     7081 gtggacgtga agaaattttc aatagtggac gacgagaaat tcatactgga tcttctgtta
     7141 caggaaaagg tcctcttggt ccagggaacc ggatttaatt ggcccggaca tgatcatttt
     7201 aggatggtgt tcttacctaa tcttacggat ctcaagatcg ccttggaccg gtttgagtac
     7261 ttcctgtccg actacagcca aaaatcatag cgattattat aacaacaaga tagtttccat
     7321 aaagagcaag ccataagacg ggcttgctct tttgcttgtc agatttaacc tattgggctg
     7381 gcccgttgtc cttttatagg attaatgcag tgggctgtgc atccgctcat acgatataat
     7441 tgggcacacg atagctgtta tctatatata cataacctgc acagcacgcc aaatttagat
     7501 aatacagaat aatccgggtt tgataactaa atatgcggtg tataaggata ttaaaggatg
     7561 ttagaaaaat cgacctgata ggtatgaatg cccgactcat gagtaagata cgtaatacca
     7621 gcggtatatt ggaacgggag gtaagtaaat tgcgaagata cgaatttttt aatagactcg
     7681 aaggtattat tgccgaaatc ggaacagcga ttttagccaa tattgacgag gacggtacgc
     7741 cgaggatgcg ctggatgacg cctgctacct taaagggcag gcccggattc atctattgcg
     7801 taggggctgg gaattcaaaa aaagttaagg ggttaaggat aaatccgaaa gcggaatgga
     7861 tgatacagag caagaatctg gattgcatta ttaacataaa agggaccgta aacatactcg
     7921 acaacccgtc tatcaggacg gagattttac acaccatagg tccacggttg gggatgtttt
     7981 ggaagaccaa cttagaggat acagaatttg tggtgctgga aactgtgatg gatgaggcta
     8041 tcttttttga gcctatgctg tgcaagtcaa aaagggtttc ttttagggag ggagacgaat
     8101 gagcaggacc tttgaaatca cgaaatacaa tgaagatttt ttgatagatc tattggaaaa
     8161 tatgattttg atacgcagat ttgaagaaaa ggcgggacag atgtacggtc tccaaaagat
     8221 aggtgggttc tgtcacctct atataggtca ggaggcggtg gctgtaggct ccatagctgc
     8281 cctggactta gccaaggatt atgtgttaac cacctacagg gaccatgggc atgccctggc
     8341 ctgcggtatg gaacctgggg tcttgatggc cgaactctac ggaaagaaga cgggctgctc
     8401 caagggaaaa gggggatcaa tgcatctctt tgatgttgaa aatcatatgt acggtggaca
     8461 tggaatcgtt ggatcccaca tacccttggc gaccggtgta gcgttgaaga taaaatataa
     8521 ggaagatgac ggtgtcgtag cctgctattt cggggacggg gctatccacc agggcagctt
     8581 tcacgagagc cttaacatgg ccagaatttg ggaccttccc attgtattca tatgcgaaaa
     8641 taatcaatac ggtatgggca ccaactacga aagaatttct tcggtccatg atttttataa
     8701 gatgggggat tcctatggga ttcccggtaa gcaagttgac ggtatgaatg tattagaggt
     8761 ctatgaagag atgttacagg ccgctaaaac cgcacgggaa tcagggttgc cgagcttttt
     8821 agagattaag acttataggt acaggggcca ttccatgagc gacccggcaa agtataggac
     8881 cagggaagag ttggatgagt ataggcgtca agaccccgta ctgctgttta aggacgcttt
     8941 gatagaatac aatgtaataa ccgaagagga ttatagggaa atggataata gatacaagga
     9001 agtagcaaac agagcgacgg aatttgctga aaacagtgaa gaaccggggc tggaaaccct
     9061 ctatgaggat atcttagcct aaagatacta aaatggttga tagaggagga tgttaggatg
     9121 cctatcatta cctatcgtga tgccttaagg gatgccatgg atgaggagat ggcaagggac
     9181 gagaatgtta ttttattagg tgaggaagta gccgaatata acggggcata taaggttagc
     9241 caggggttat gggataaata cgggtctaaa agggtcatag atacgcccat caccgaggaa
     9301 ggttttacag gcgtcgggat tggggcggcc atggcggggc ttaggcccat agtggaatgg
     9361 atgaccctaa acttttctat attggcgttg gatcaagtta tcaacaatgc cgccaagatg
     9421 cggtatatgt caggtggtca gttcaaggta cctatagtat ttagggggcc caacggaccc
     9481 gcggaatatc ttgcggctca gcattcccag gcccttcaaa ccctttatgt ccacataccc
     9541 gggctcaaag tagtagcccc cgccaccccc tacgatgcca agggcctgct aaaatccgcc
     9601 attagagatg ataatcccgt aatattttta gagggcgaac ttatgtatca atggaaaggg
     9661 gaggttcccg agggagaata tctggtacca ataggaaaag cggatataaa gaggaagggc
     9721 caagatgtaa caataattac ctattcgaag cccttaagga ttgtggaaga ggccgccatg
     9781 gagctggcca atcaggggat tgaagccgaa atagtggatc ttagatcctt aagacccata
     9841 gatgaggaaa ccatctatcg atccgttaag aagaccaaca gatgcgttgt ggtggatgaa
     9901 tcttggccaa tggtcagcgt agcttcccat gtgggttgga tggtatccag gaactgcttt
     9961 gacgatttgg atgcgcccgt agagctggta accggcgagg atgtgcccat gccttataac
    10021 cacaggctgg aattagcagc ccagccttct gcagggaagg tagttgatgc ggtgaaaaag
    10081 gtcttgtatt tgtcgtgaaa agaacaggag ggatattatg gctgagaagg tattgatgat
    10141 tgccctatcc cctacaatgg aaacagggct catttcaaag tgggaaaaga aagaggggga
    10201 aagggtagaa tcgggggatc ttttatgtca ggttgagaca gataaggcta ccatggacta
    10261 cgaatcaata caggagggca ccctgcttaa aatattggtg cgcgaggggg aacaagcggg
    10321 cattggctcc cctattgcga ttataggcga gacgggggaa gatatagccg agatattggc
    10381 tgaaattgaa gcggagacca aggattcgcc aagtgatggg acagaggagg ttaaagaaga
    10441 ggttgccgcg actcccgcaa tagaagaggc agaagaatac accggcgaca ggatcatagc
    10501 atccccgttg gcccgtagga tggctgagca tcagggtatt gatttgacct atattcaagg
    10561 cagcggaccc agaggcagga tcattaagcg ggatatactg gaagctgcat cccagcctca
    10621 aatgcccggg actcctacac caaaatctat ccccaggatt ttaccgactg ccgcggatga
    10681 agtcgtaccg gtatccatta aacgcaaaat tattgcagac aggctggcgg aatccaaata
    10741 ctctgcgccc cattattatt taaaattgag ggttgcaagc gatggtttta tgaaagcccg
    10801 aaaaatgctc aacgataccc tcagcgaaag gatatcggtc aacgctttta taattaagtt
    10861 cgtcgccgag gccataaaaa ggcatccggt tattaactct acatggcagg gtgaaacgat
    10921 tttgggacat ggcagcattg atattggttt ggctgttgca caaaccgacg gtctcattac
    10981 cccggtggta agggactgtg ggaataaggg gataatagag atagataaag aattgaggga
    11041 tctcatccat aaagccctta acaaccagct tacgccgccg gagtattccg gggctacctt
    11101 taccgtaagc aatctgggaa attacggaat cgaagagttt actgccataa tcaacccacc
    11161 cggttcggca atattggcca ttggcaagat gggaaaagaa cctgtggtgg tagacgggga
    11221 taggatagag gtgcaatctc tgatggcttt gaccctatca tgtgatcatc gggttataga
    11281 cggtgccgtc ggtgcggctt tcctaaagga tttgaaggat ataatggaga atccaataag
    11341 ggtactatac taatgcaaag agccttaaaa tccgcttttt aagcggcagg ataagttccc
    11401 ggttttccca aaacccggga tgtgacaaaa cgtttaatag ccgttatgga tatgcccaat
    11461 cgatagtgga ttgaattttg ggctttaatc aaggctattg ggatatttat aaaaatatct
    11521 aaaggggata ggattatatg gatacgaaat ttgattatga tatattgata attggttcgg
    11581 gacccggcgg ttacgtagcc gctatccgtg cctcccagct ggggcttaag accgccgtga
    11641 tagagaggga taagcccggt ggggtatgtt tgaatgtcgg atgtattccg tccaaggcct
    11701 tgataaacca ggcaggaatc tatcaaaaca gggcccagct gatgaatatg gggattaagt
    11761 tggacgaatc cggatttgat tatggcaagg tatttaaaaa gtccagaaag gcggccaata
    11821 ccctggccag gggggtcgaa tacctcctca aaaagaatgg ggttgaactc attatgggag
    11881 agggttttat cgaatctccc aatcgagtgg cggtagggga caggagctta ttggctaaaa
    11941 acataataat tgctaccggt tccagcccca gacaaatacc cggctttccc gtagatgaag
    12001 tgaaggtctt atcgtccaca ggggcgctta tgctggagga attaccgaag gatatagtta
    12061 tcttaggcgc gggggctata ggcatggaat ttgcctatat cttgaattct tttggtgtag
    12121 aggtacatgt ggttgaaatg atggacagca tattgccgtt ggaggataag gagacagtgg
    12181 aggttttgcg aaggaccctt cgaaagaccg gcataaaatt ttatacctct actaaggccc
    12241 tatccatgga agaaaaggat aacgggagaa tcctcgttgc attggaagat gccaagggca
    12301 atgtaacgga aagggaggca gaaaagatcc tggtagctgt gggcagaaaa cccaataccc
    12361 acggaatagg tcttgaaaac ttaggtatag aaacggttag agacttcgtt cccgtcggag
    12421 attattacca aaccaaggtt cagggggtat tcgccatcgg tgatgtcgtg gattctccgc
    12481 tactggccca tgtagcctcc aaggaaggtg agatagcagc ggagtttatc gccgggcata
    12541 agcccgagcc gagaattgac ttaaacgccg ttcccagcgc tgtctattgt gagcctcaga
    12601 tagccagctt tggatttacc gaggacaggg caaaaaatga agatataccc tatgagaagg
    12661 ctgtctttcc ttacaggggc gcaggaaaat cagtagctat agaaagatcc gagggaatgg
    12721 taaagatcct ctatgatcct gttacccatg aacttttggg cggccatata gtcggggcag
    12781 aggccacaga gctgatccat gagctgttat tggctaaatg gggagagatg ctgcccgagg
    12841 atatagcagg catgatacac gcgcatccga ccctggccga ggcagtgatg gagacagccc
    12901 gcagcgcaga gggctgggct atccatatgt aacgaacagc gaaaactaaa attccgccat
    12961 tatgtttacg tgcacttaac atgctgtgcc gatttgtcat attgttgtct gtaggtaagc
    13021 aactagagct ataggttatc atcatgaaac tgtatatacc cattgggatt atcatgccga
    13081 taaatttagt ttccgtaatg tttatcttca ataaaggcaa tgaatcattg aaaagcggcc
    13141 ggtacagctc gttaacccta acgtaataaa tattgcggaa tttttatatt cccgacaatt
    13201 tacggactgc agataataag aatcccaaaa caaaggaagt atatttttta tttgaagaat
    13261 tataggaaaa tcatattgtg aaagggctcg ggactcaagt gaatggaata ttattctcca
    13321 aaattagatt aagtacccca aaagaaaagc agagtagaca agatatcatt tttaggatat
    13381 tatactgata atatcctttc ggtattgtca aagcaaagat cggccacttg agttttaaat
    13441 atgggctcca tttaagtttg gcaggggact atataaatat tgcttttgaa ccaagaccat
    13501 tattcaattg acacactatt acagataaag aggatttgct cggcttacac cattatattt
    13561 gggggatagg gaatagatga tagagaggga attcgcaata aaagatgata tgggcttaca
    13621 tgccggatcg gcagcgaagt tcaccagaat tgcgggcagg tataaatcgg agataagggt
    13681 tatcaagggt aacaagaccg gtaacggcaa gagtcttctg tcgattttag ccctgggcat
    13741 ttttggggac acaaagttcg tcgttaaaat agtagggcct gataaaattg aggctatgta
    13801 tgaccttact cgattaatag agaacgattt tatattggac tgatgaggtc catggataga
    13861 aatttattat gagggataaa agtgacggaa ctgggtatta tccacgtgga tatggatgct
    13921 ttctacgctt ctgtggagca aagggaccat ccggaattta gaaataagcc tgtagtagtg
    13981 ggaggagacc ccaaaggaag gggagtagta agcactgcat cctacgaggc gagaagattt
    14041 ggggttcatt cagccatgcc atgcaggcaa gcagcggaac tatgtccaaa tattattttt
    14101 ttacctgtgg acatggaaaa gtacaagaga gtttcgaaaa agattcattc aatctttagg
    14161 atctataccc caaccatcga acctatatcc attgacgaag catttttgca ggttgacggg
    14221 ggagacccgg tggacatagg gaaagagatt aagggagata tatataaaag acttcatctc
    14281 accgcctcag tcggtgtttc ttataacaag ttcatcgcca agatggcatc ggacatggaa
    14341 aagcccgatg gatttactgt tattacccga gaagaggcca gaaaaatatt gcccgggtta
    14401 cccataagga agatatgggg agtaggagaa aaaacagaaa aggatttaaa taatatcgga
    14461 atctttactg taaacgactt actgcaatac gaccatgaat ttctccttag gagctggggg
    14521 agaagggcct atgagctcat acaattatgt cggggaatag accatagcat ggttcagata
    14581 gatcaggaaa caaaaagtat gggcgaggaa acaacccttg agggggatac cagggatctg
    14641 gatgtcttaa agggaatcct aggagatttt tctatggagt tgggtaacag gatggccggc
    14701 aggggtataa aatgtcgtac cattacaata aaagtaaagt ataatgactt taagtccata
    14761 actaggagta taaccctggc cgttgctacg gattcttccc atattattct tcaacactcc
    14821 cgggacatgt tggctaatcg ggtatcactt tcaaaacccg tcagactcat aggggtgcag
    14881 gtctcaaact tggtttaccc cgatgaaccg gttcaaattt ccttcatata gcgttcccat
    14941 cgttgatttg cccctatatc tgaataaaaa accctttaaa ggaaagtatt catatatagg
    15001 caacaaagag gagggaactt atggaagccg gtcttatgat caagatctat acacaggaag
    15061 ggaataaaaa actcaacaaa caatatttgg ggacgaaacg agccattagc caaattgcat
    15121 acgaaaaaat gcaggaatac atgcgcaccg cggatgtggg tttggggtca gatgaaagga
    15181 atatattgga gattctgata gccaatagta tgatgcagag cttttctctg ggttatggta
    15241 ttggtaaagt ggagggtctg accaacaagc aaattcactt gtagcggccc aaggaattga
    15301 atcagagaga gcagatgaac gaagaaaata agaatagtat tagtcccgct cttatgatat
    15361 catgtgatgg gggaaatgat taatattttg tttgggtaca atcctgccgg aatgtaaatt
    15421 ttatttaaaa gacttgcgcg gtataatcaa gtatatgttc acgaagggtg ttttatgtgg
    15481 gggtttaacg ggaaattaaa aaggtccatg ggaggatata ctagacttgg aatcttagtt
    15541 attatacttg ggattctgat tgctactatt ctcctgtttg gcgcggccaa atttaccgag
    15601 gacagatcct tttttatact atccccaaac acatttttag cactgcacat actcttagaa
    15661 ttcatggcta tagtaatggc cttttgcatt tttgccatca cttattacac ttccggggag
    15721 acaaaatccc tgtcaatgat cattatagca tgtgttttct tggcaacagc gcctttagat
    15781 gccttgcaca cctttaccta caaaggaatg ccgcttttta tagtcacgcc ttcagccaat
    15841 accgctacgc tcctatggat agcctccaga ttcataatgt ctacgggcct cctcttggca
    15901 agccttattc ctgaggatag ggggacgaag ggggaccaaa gcccttatgt cgcgattacc
    15961 attggattga taggactgtt aatgggattg gtgttataca gacccggtct gtttcctcct
    16021 atgtatacag aagggagcgg gctgacaccc ctaaagatag gccttgaata ccttataacg
    16081 gggatgctgc ttgttacggc aatggtctat tacgggaaat tcagcagaag ccatggggat
    16141 agggagtatt ttctcttagc cattggattt tttataagcg ggttagggga attagccttt
    16201 accctatata gcgatgtata cgatacctac aacttattgg gacatatgta taagtttctg
    16261 gcccgctatc tactctttcg ttctctgttc gttataaata tcaaaaagcc ctacagaaat
    16321 ctttgggagg cagaaaagaa attaaacaac tatgtcgacg atctggaaga atcggtaacc
    16381 caaaggacta tggagataag aagggccaat gaaaagctcc tcaagaattt aaaggatgca
    16441 aagcatattc aaagggcttt gatgaccaca gaatttccca aaatatcggg aatggaattt
    16501 gcggcgaaat accttccctg tgaacaggtg ggcggggact tttataacat attcaaattg
    16561 gatgaacata atgtcggtat tgtaataggg gatgtggcgg ggcatggagt atccgccgcc
    16621 atggtcaatg tttttatcaa tcagaacatg aggttcaggg tggattatga cgaacaaaga
    16681 taccgcattt taaccccgcg cggggtattg atgaacttat atcatgtcta taataatatg
    16741 tcctttccgg atgaaatgta tgttgtgctg ttttacggta tctacaatac ggtaacaggt
    16801 aagctgtcct actcctctgc aggaatgaat acctatccgc tgatcctgag tgcaaagggt
    16861 gagatatccc atattaagct ggatggcttc cccatatgca agttcggtaa gttcttcagg
    16921 ccggactatg agaccaagac attacagcta tcatccggtg atactctgat tttctacagc
    16981 gatggcttag gggagatcga tcgaaaacag cccgatatcc tatcatcgga gaatattatg
    17041 gaatatttaa gggggatgag gcattactca gcccgggaag tttgccaagg tttatttgac
    17101 gcttatcatg ccctcctggc ggataacgaa atgttggatg atgtaaccat actggttatc
    17161 aaaacaccat aaagcgaact gttttatata aaacggtgcc ggttgatgga tttcaatcgg
    17221 caccattttt atcggaagaa tccttggaca ataattgtgc catattcatt ataagctcca
    17281 gggattttgc ttggtccgga tttaatacgc tcttaaaggc tttggatatg ttttcgacct
    17341 tttcatccct ttcttttgtc gcatcccggg ttgccccgga atcatcgaag gattgtccct
    17401 gctctttccc atccacggcc cctgctccat ccataacacg cgaggaatcc tgtatggaag
    17461 gtgtaatttg ttgggccagc tctatggtgc ctacgagcct aagaacgttg ctgacctgat
    17521 caatgtattg cagttgttcc gccggggcca atatcttgag aatatcaatc atttccattg
    17581 ctctggatat tttatcggcg gcggaatcct gtgcttttat gctatttcgt tccttaaacc
    17641 tttctatgct gtccaccagt ttttctaaag tattatttac atggagtatt ctgttgatct
    17701 gggtctggtt ttccaaagaa atatgcggtt gtaacgcttt tataatcccg acagctcttt
    17761 ccttgtagct tgtaatgggc tttgatgcac cggatatacg cggtgtttgg tatgtaccgt
    17821 ctatcaggcc ttggagattg gcctttgttt tggccaagcc cacaataaag tttataccgt
    17881 cctgatgttt tgggtccaga tacggataga tagaagtcaa aatatcaaca accgtatccg
    17941 atccgtcagc ttggctttcc tcgctgttat acagtcgaat cgaatcggat ctggctctct
    18001 gtatgtaggg agagaccagt agaatgagta atataatggg tagcggattt gcttgagccg
    18061 gcggagcata ggtgaatccc gggggcatct ttttttgagt tccggatctt ttatctgcat
    18121 ctttgggcaa gtctattgtg ttgccggggt ttattttcgg gtttatgttg ctgctataac
    18181 gtagggggat atagcggtcc ataggacacc tccttgatta tagggtaatg gtctctatta
    18241 aaagcatact atttacatct atgcccatca tccatggttc tatgtaccat attatgctat
    18301 aggcaagaaa atgtgcgttt agggtatcat tgaatatttt gatgaatagg atgataagta
    18361 gagattataa tttggtgaaa ggcggaatta agatggatat aaataaggat agtttagaaa
    18421 caatggccgg tctcttcggc aacctggagg ggaagtcaga ggatgaaatg atttccgagt
    18481 tggcccgaat gattagggca ggccagggcg gaataacagt ggacaaagct aggcagatga
    18541 ttcagactat tctccccatg acggacagca atcagaagag aaagctggaa aaactgttga
    18601 gaatccttta gccattaaag cccgccagcc tatatgctgc gggttttaat tttatagctt
    18661 atctcgccta aagccttcac acatgccttt acctcatcca gggtgttaaa ctctccgggg
    18721 cttaatctga cggtgccctg ttccttagtg cctatggtct cgtgggcaag gggggcacaa
    18781 tgcaggcttc ccctggctgc tataccataa tcggcattta atatatttac tatctctctg
    18841 gaatccctgt cccatacatt gatagatacg atacccgatc tatgcttcat atctttaggg
    18901 ccgtagactt taatacccct tatctgccat agggcattga ccagcagctt ttccatatta
    18961 gccacatgat ccagcgccct gtcctggcta ttacccagga tatatccgat accggcccca
    19021 agacccgcaa tccccggcgt gttcagtgtt cccgattcgt agcgatcggg taaaaacatg
    19081 ggttgatata cgctctccga ttggctgccg gttccgccct cgcggatttg cttcagcgcg
    19141 attccctcct ttacataaag cacccctgtc ccttggggcc ccagcagccc cttatgtccg
    19201 ggcattgcca ccaaatcaac ggataacttt gatacatcaa tcggtacgag gccggctgtc
    19261 tgtgccgcat ccagtaggaa gtatacaccg gaagcttcgg caattttccc gatggcctcg
    19321 atgggcatca gggtacccgt tacattggaa gcatgggtgg agataattaa gcgcgtatta
    19381 ctccggatat tctttttgat atcatcaggg tctatgagcc cttcggagtc tgcagggacg
    19441 actgtggttt caacccccag atcggccaga tgcatcagag gtctaattac ggagttatgc
    19501 tccattgaag tggtgattac atggtcagat ttattcaata tacctttgat agccagattc
    19561 aagctatccg tggcgttata tgtaaatatc attctgaagg ggtctccggc gttaaatagt
    19621 tggcagagca tctccctggt atagagaagg atgtttgccg cttctacggc catgttatgg
    19681 ctggagcggc cggggttggc accatagtac ttcatggtct tcataacagc attgtataca
    19741 acatccggct tgggccaggt cgtggcggca ttatccatat agatcatttc catctttcca
    19801 tcccccagtg taacaaaaat taaatacata aatatattgc taatagggct gctccatccc
    19861 aaaggggtac cacgattaaa ccctatatac ggcacagtat tgtagcgatt cgactctttc
    19921 ctgtagaata tattattctg cggttatgct tgccctgtag gtgctgtatt tataacgggt
    19981 atagctaata tttctgacag acagcggcct aagccaacaa ctaaaatgtt aagaaaataa
    20041 ataggtcatt acgttagggg gtaacacatg ccagcagtcg aagaatttaa ttttatatcc
    20101 tttaattccc ggcaacatgc actctacttt gatcagctac tgagaaatgc cgggtacgat
    20161 tcccaaatta tctccacacc taaaggtgta tccctgggct gtgggctatc ccttagattt
    20221 tcgcctaatt taaccggtgc gattatagat atttacaggc gtaaccagat acctataatt
    20281 ggtttctatt caatcaggcg gatgggaagc tatagccagc tacggaggat accgacatac
    20341 tgaaacaccc tagtgcagca tagacccaaa aagtcttttt aacgggccct tgttctgcat
    20401 cttcctaatc tttattttga agtcatccaa ttcttccagt atgagctccc aacggcgttt
    20461 ggtctccata gaacgcctat ggagctcatc ggtctccctt gctatggaga ggtggtctct
    20521 gtagacagcc tgggctttgt taatccattc tagggcatct tcatatatac taggctcggg
    20581 cgaaaaatta tcgcctatat cagccccggc gtatggatta ttcgggagcg ctagccactt
    20641 ttgaaggtct gtgtgatatt ttacatggag caccttggat atgcttccgg agacctccct
    20701 gtaggataga tggatgctgt tatcgtaaat cctatagctg tatggggccg attcccagtc
    20761 gtcgataaat tcagatatcc tttggtggac ccacagtttc ccatcccagc agtagtgata
    20821 cagagaccat atctcagctt cccacgggtc gcctactgcg aagatgatat ggattctatt
    20881 atccagcgcg atgacggcgg ggtatcttat atcatattct tttacgttgt attcgctgag
    20941 tttttgctcc gaccaccctt tatcgtcata gtgcatatag gttataccgc cttccaggga
    21001 caggtatata aaatgtaagc cgccatgctc gtctacacaa accccgaact cattttttgc
    21061 attttccgat actagatggg ggcccatcca acccatattt ccttccaggg tcctgtaaca
    21121 aatattattc cggccatctg aaaagaagtc ccacattcta tcaccatcaa accttatcaa
    21181 cctatgcaca ctttgatcgg aacccatctt ttctcccccc ttttaagtat tttcataggg
    21241 atgattgacc attcgaagcc ttgatatatc ttagatgccg cattacatcc ctactgattg
    21301 agcttcgtgg gctaagggcc taaaaaaggc cattcaatat caccttgtaa taatatgtag
    21361 ataaacaata tggaaactaa atttgccggc ataacgattt cagtgacctt gtgcaacttc
    21421 atgatgataa tttttacagt tggagttgtt tatctacggc attctgatat attatttata
    21481 taaggcaatg tacatgttta taactaaaat atgaactttg acttaggacc ggtgcagatt
    21541 tattgtgccc atgttccgtg acagcacaaa aaggggaacc cttaaggtag atatcgccga
    21601 taattccctt taaatgataa ttaggagtgg atagcttaga tgagcaataa aataggagat
    21661 atagacaaga tactcgagtt gttggaggaa aggtatagcg gcgccggccc cgagcttcac
    21721 cacaaaaacc cctttcagct gttaattgca acgattcttt ctgcgcaatg tacggacagg
    21781 caggtaaata gggtaacccc caatcttttt aaaagatatc ccaaggcgga ggatttggcg
    21841 gctttggatc cccaagaatt ggaaccccac attaagagtt gcggttttta taagacgaag
    21901 agcaaaaata tcgttgcaac gtgcaggctc attttggaaa ggtttggcgg ggagatcccc
    21961 agggatattg agacccttca aaccctgccc ggcgtgggca gaaaaacggc caacgtggtt
    22021 gccagtaacg cctttggtgt agatgccatc gccgtagata cccatgtctt tagggtctca
    22081 aaccgattgg gtctggcaaa ggccggcaat gtgctggaga cggaaaagca gcttatggac
    22141 aatatcccca aggataagtg gacggcggcc catcactggc ttatccatca cggcaggcag
    22201 atatgcaatg ccagaaaccc aaagtgtgat gtttgttttc tgactccata ttgtccgtac
    22261 tataacgatt agacagctat tcatcgcggc gaatgtgtgg agtatcttat acatgattac
    22321 aggacacccg tatcccataa aattagctaa atgggatgcg ggcatgccag ggtgtattgg
    22381 gccaaatcac atgatttcca tacgtgaaag atgggaatag aatcagtcaa tttgtagcct
    22441 ttttttttcc gatgtaggtt ttgcccttga cgggtctata gtagtgggta tcaaagagct
    22501 cttccccaac aagctcatcg tcttgaaacg tttgcttgaa caccctcacc ttgtagccat
    22561 ttctagattc cgtatggata tactcctccc cctcaaactc tgtatacctt gcctcataat
    22621 ccacgatgat atcaggtgcc ggggcgggta tagtttcata gacttcggaa tagaattcgt
    22681 atctttgtcc ccggggtagg ggctcaccgt atatttcgaa atacacccgc ttgtcggcaa
    22741 agtaggccct tatgtatatt gggcttcccc tgttatttcg aaatttcaga tcgattgtgg
    22801 gctccggcgg aggcaagttt accgtcgtat caagacccat atctatgtat gatgagggaa
    22861 gggaatggcg ggttcgttct ataatatcaa gcccggccct aatggcagta ttatagagtg
    22921 tagttgaggt ttgggaaacg ccccctccta aaacgtcgac aaaccgccgg ttggaaatca
    22981 tgggagcata cccatagccc ttatccgagg tgcgcttgcc agttacctcg ttaaaggata
    23041 ccgtctcccc gggggatacc attaggccgt taaaggccag tgccgcagtt ataacattgt
    23101 ttgtccgatc tgccgaactc ttggataaat ctgtcccaaa ggcgattatt ttctccaatt
    23161 taccgtcgaa atctttggct aaaccttggg agtcaatcta tttggagaca ggattatggt
    23221 atcggttgtg gttttaccgt ccaatacttc tcttatttgg cttataacca tgctcgtatc
    23281 tagggaaagc ccttcctctt cggggcttat attgaatttt ttctccccat ccggattgaa
    23341 ggttacggtt gcgtcgactg tgtctcggtc tattgccgca gctactctct caacctcagc
    23401 ctgcaggctg tccacgctat aggttagttt cgtatggaag gagcgcccct cccttctcaa
    23461 tctttggacc ataaggaaac gctttaaagg atggcccgtc cttgcgatgc cgtaggcctc
    23521 cttcgtcttt tcgcttatgt ctatagaaac ccccatatcc ttgtatccca gggtccagaa
    23581 atccgattga tgcaaaagtc ccaccttcag attatccagc cttgcctgat ttttttccct
    23641 taacaatttt tcggcggatt gtttagtcaa gccgcccata gataagccat caatcgttat
    23701 accctcataa aaggtatcgg tatctaaaat tctattaata taagccctta gcccagtgcc
    23761 cgccataagg ataagagcga taaatagtat aactatggca attggtttct tcctctgtcc
    23821 ggtagttttt ttgcgacgtg ttctcataac tgatttttcc ccttctccaa ttcgatgtcg
    23881 gaagcagcgt tctatagtca ttatcgacat gtttatagac acacaactcc aactacagaa
    23941 tttcttaacc atagataaat ttagtttctg tattgcttat ccatagccag cgcaacaggt
    24001 taataatcta agtatagatt tccttatcgg tcataagtta tgtataccaa atacattgtc
    24061 aaatgaaacc ctcggcaatt atcgctatga tttttatgga atctatgtta aaattaatac
    24121 aacgtggatt gaaaaattat attatgggta tatgggtctt caacttattc aagacccgag
    24181 ataacgatat ttggggaaat ctagatattt gtccgcgact gagcggcggg agggttgtaa
    24241 agtttgagga tctatggctt ggttggaccc agcggtacag gcaagagcta taaagcagcc
    24301 aatcttgcta acgagcgggg tattt
//
LOCUS       DASRNX010000137        32262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_714501,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000137 DASRNX010000000
VERSION     DASRNX010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 32262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32262
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714501"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VFD89_05605"
     CDS             <1..709
                     /locus_tag="VFD89_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HZJ57701.1"
                     /translation="YNIVGVLLTGFINGLNPCSFSMLLFLLSILVAKNVNILKIGFTF
                     IVGKFVTYFLLGTLLFNIFVRLDIPHFQIMSKVIMAVILVFIAILNLSDSFAARREDY
                     ERIRMQLPIFLRKKNHQWINKFGNTIDQTFLMPLSLMLGVLVSLGEFLCTGQIYLATI
                     ISILHDSKTLDYRAIIYFILYGVSFVLPLILATIFVYKGRQIFELTEWLRGKMPVVKL
                     VNAIIFVLFAVFILIWL"
     gene            721..1050
                     /locus_tag="VFD89_05610"
     CDS             721..1050
                     /locus_tag="VFD89_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57702.1"
                     /translation="MRNITISFVGKSGSVLSHKKLLELPLRNEFIIGRSIEYFNDPEP
                     CMIHRSAVIKRIYMELFDYLGGIYTSREVEMLWDILPQKFRKAIDIPQEPHLIRFSFR
                     EDNRRKR"
     gene            1438..2523
                     /locus_tag="VFD89_05615"
     CDS             1438..2523
                     /locus_tag="VFD89_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ57703.1"
                     /translation="MSKEQKSRRIGIGSIGLGGISKGVHLPGIERSEDFKLVALCDID
                     PEALKAAQEKYNIPDSRCFTNYQDLIECPDVEAVDISTPNDSHFEIAYAAAVAGKPYA
                     LEKPITMTKEEADKLAEITEEKGLKSMVCFSYRFIPAVRYMKDIIDRGLIGDIYHVNM
                     QYFQAWGLPDANCPLVWRYIKDRTGSGALGDLGSHGIDLVRFVTSKEYKKVIGHADTF
                     VKERQVPGSDDMGKVDVDDFCNYMAEMDGRTSANFQITRFAYGRGNYQRMEIYGSKGA
                     MVYHLDRSPNANEIEVCIGEINREARLFTKLPVPDKFKADQMQSFADIINDKGDGLAA
                     TIFDGQSNQHVLDSIIESVEKGEWVTL"
     gene            2603..3331
                     /locus_tag="VFD89_05620"
     CDS             2603..3331
                     /locus_tag="VFD89_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018757266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZJ57704.1"
                     /translation="MGKIKVTVWNEYLHELQVPEIGEIYPDGIHNCIGDSLKDAGMDV
                     KTATLRMPEHGLTDEVLDDTDVLVWWGHMAHGEVEDEIVEKVYEKVLGGMGLIVLHSG
                     HASKIFNKVCGTATGMLKWREDGDKEILWVVDPSHPIVDGLDEKIIIPNEETYGEHFN
                     IPNPDDLVYISWFEGGEVFRSGCCYYRGKGKVFYFRPGHETFPIYHMPEIQKVIANAV
                     KWAAPINFPDLTYGNTKPVVEIKK"
     gene            3826..4743
                     /gene="trxB"
                     /locus_tag="VFD89_05625"
     CDS             3826..4743
                     /gene="trxB"
                     /locus_tag="VFD89_05625"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZJ57705.1"
                     /translation="MKDIIIIGGGIAGLTAGMYAKRAGMETILFEKLFPGGQAATTFS
                     IENYPGFVEPISGPDFSMKLEEHARKFGLEILYDGVDEIIIDGNIKKVVTDGGTYETK
                     TILLATGASPKELGLEKEEQFRGRGVSYCATCDGTFYKDKVTAVVGGGDTALEDAFFL
                     AQYASKVYLIHRRDEFRGLKILQDRVLKHEKIEVLWDTVVESITGEEQVEGIGVRNVK
                     TGQTTELSLDGFFIAIGITPNSELAKGKLNMTPDGFIIGDESMNTNVSGVFAVGDVRY
                     KPVWQLVTAASDGAVGTLSAQRYIMEHFE"
     gene            4861..6207
                     /gene="glmM"
                     /locus_tag="VFD89_05630"
     CDS             4861..6207
                     /gene="glmM"
                     /locus_tag="VFD89_05630"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZJ57706.1"
                     /translation="MGRLFGTDGVRGLANEKLNCELVYKLGQASARVLTLAKYRPRIL
                     VGRDTRLSGDMLEAALVSGICSVGSEARVVGVMPTPAVAYLARHYKADAGIVISASHN
                     SMEYNGIKIFNNQGYKLSDELEEKIESMILDNTESITYPTGINVGRIVRVKNASRHYE
                     SFLKGTIDISLEGLKLVVDCANGASSNIAPRIFSELGARVHTIYDDPDGININNLCGS
                     TYPDKLQDHVIETGADLGLAFDGDADRLVAVDENGELVDGDQLLVICGLNMKKRGLLK
                     KDTVVGTIMSNLGFDEALKSAGCRVVKTNVGDRFVLERMLEEGYNLGGEQSGHVIFLD
                     YNTTGDGILTALQLLAILKRENKPLSQLASAMEKYPQVLINARVDEIQKRKYTDDPEI
                     QKEIKAVEDRFKGRGRVLIRPSGTEPLIRVMIEGKDQREIEEHAVALAGLIEERLK"
     gene            6508..7701
                     /locus_tag="VFD89_05635"
     CDS             6508..7701
                     /locus_tag="VFD89_05635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZJ57707.1"
                     /translation="MYNRRILAFALILVMAVLMGCASKAGNDDLKDRTVVGKVNGEKI
                     TKGEFNSLYNQMKQNYYISEEVEQDPDQSEMIAELKLDILDQLVIEKLMAQKAKGAGF
                     EVTDDILEEARAEFEEIVIHLAGQMEIIDGDTQDEQGQETDDTGKDYIEEAKAYIEQE
                     LAAMGQSQDDFIDSLAKQMTVDGYIESLLEDISADKDEIEEYYRERLQNQKSNIAGIN
                     YEEVELYKPEEVRVKHVLIGLPKEELERYDELMGDGKEKEAKKHLEDKLKEIEPKAME
                     VLQKARDGEDFEKLIEEYGEDPGMEDNTPGYIVRQDGDFLPEFEKAAFTLEEGEISDL
                     VATDFGYHIIKFYERYPQKVYTLEEKHDEVKEFLDYEKKADAWTTILDEWMADADIKR
                     YENKL"
     gene            7752..8444
                     /locus_tag="VFD89_05640"
     CDS             7752..8444
                     /locus_tag="VFD89_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZJ57708.1"
                     /translation="MKRKVLIVDDESSIRSFIRVGFERSNFIIIEAETGEDGIELARE
                     HMPDIVILDIMLPGMDGFEVCIALRSEFPDMGIIMLTARDLDMDRIMGLERGADDYVV
                     KPFNPLELVLRAEALLRRIGTRDGQGDDRRLWDEPFEIDTYSQKVYKRDLEIDLTPKE
                     YQLMTLFVKNPGRLFSRNELIDTLWGSDFMGDLKIVDVNIRRLRKRIEDNPSHPMFIE
                     TVWGVGYRWREK"
     gene            8441..9856
                     /locus_tag="VFD89_05645"
     CDS             8441..9856
                     /locus_tag="VFD89_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ57709.1"
                     /translation="MRKRRKSIRTRLSYSFMSIIIISIFIFIFMVSLFLKQYYYKGVE
                     DILTDQVNMSVNLYSQYFIDSSLQDNVMDNVDAFWRFSSAQVQIIDNRGRVLMDSIGM
                     DFTDELDTPDVKRAMMGEWGKWIGRMPYTGEMAMSVAQPLKSNGDIIGVLRFITSLTP
                     INGQLEGIIRVFMLIGILAVGISGIISFVLANGVVGPIKQVTKVAEEMAKGDFDVRST
                     EKTHDEIGRLSSTLNYMADEITKKERVKNEFLASVSHELRTPLTSIKGWAIILNDTEN
                     KEEKIFRDGLKIIEKETERLSGMVEELLDFSKLLSGNISLKLVETDIERLIEYIKQHM
                     MPIAKRNKITFRVECPDKLPCIVVDSNRIKQVLINVLDNAFKFTSQGGNVRLAAYGAG
                     DDLVMEIEDDGCGISPEELPSVKEKFYKGKTGKSSSGIGLSVAYEIVKKHQGGLDIES
                     RPGMGTRVYIRLPIAHGTGRN"
     gene            9871..11385
                     /locus_tag="VFD89_05650"
     CDS             9871..11385
                     /locus_tag="VFD89_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57710.1"
                     /translation="MRFINVSSIILICILFFAGCQGIETLNSAQTIIPPENNRISIQG
                     TWKIKDYKRMSDLQEENDAVAGSEELLGEIALFAHDWAVVVDESCTEAQYQVRRVNAV
                     DYFLFHHNTDVQELGVENEMIEIISVTSNGVLFFDILKMDDDTAIIYMDKCFYWLEKV
                     SDNIERSRNEYGTGTNKEDVNRYEEEKIVRSGVFLGLRTTIGPGDDGYGNRSAYRTIW
                     ISSNNRRIESVLETSNLFIPRRSGFWKLDVGTRRIGGFLQDYFDLSPVDSMGVSPGES
                     GNAGELLSFVGKNLKKNILFISDDYIAIEYSETAKDEIREPDKYMVLPLDDANSQKGI
                     KISNFADEGMEDIFHKSAQSFLVSKGRKMTGDLRESAGEENFTVARRNGHWILRGRLN
                     IDGIDEDFNIGLMPAGKLLNYDKLHVSWDDIKGKIPMALDAYTSPAKDILIAVTGNFL
                     MVYTIEDGRISEKPIKKIGIKKGETVIMAEWATGDYVARWEKGFNQINPFVVNE"
     gene            complement(11546..12739)
                     /locus_tag="VFD89_05655"
     CDS             complement(11546..12739)
                     /locus_tag="VFD89_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZJ57711.1"
                     /translation="MKKMGKGSFSKTIQVFSIILLCIFLSSCSFRYINIFSLKPSSPG
                     APANKPADGPPSSWEDNNGAGQAPETTEDKGDADTEPSSSDAKGDGDNGPSSSDDSRD
                     QDNTAPYDNIHAHKDTGIGNVKDLDGESDETINNNITAPADGNIGNAEGDNKKPVDID
                     GDGNPHGPDEDIKQQIEESSGFDDIDAVPAAKIIRNSSNDELKQVAITFDDGPDDYFT
                     PQVLDILKEYDIRSTFFILGSVAQNNRDILKRIDDEGHVVASHGWGHKNFTKLSKNKA
                     IQELKRTNELIEEVTGKSNTLFRLPYGAFNKKVLKTVADEGFHNIYWSIDPRDWSGIS
                     PNRILNDVKKNLKPGAIILLHSSGSEKSIPNSIKALPKIIEYIQGQGYDIVTIPELLR
                     DTFTE"
     gene            13196..14263
                     /locus_tag="VFD89_05660"
     CDS             13196..14263
                     /locus_tag="VFD89_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZJ57712.1"
                     /translation="MEKLTTYERIKMMYEHRDADRVPIFDSPWTATIKRWHEEGMPEG
                     MDYRDFFQLDHIATIEVDNSFRYPYKLIEETDDYITYTSKWGVTQREWKNANSTPEFL
                     EHTVVDEESWLKVRDKMTPDRDRINWKHLESNYKKWREQGWWIEGLLWFGFDVTHSWM
                     VGTERLLFALLENPEWCMDMFNHFLDVNIGLLDMVWDAGYKFDSVIWYDDMGYKHNQF
                     FSLKTYREVLKPVHKKAIDWAHSKGVKVRLHSCGDINPLLPDLVEMGIDALNPIEVKA
                     GMDPVGLKSKYGDKLVLHGGVDALLWEDIDKMEKELRRILPALKENGGYIFSTDHSIP
                     SNVSLDQFRYITDLIKDIGRY"
     gene            14591..15799
                     /gene="trpB"
                     /locus_tag="VFD89_05665"
     CDS             14591..15799
                     /gene="trpB"
                     /locus_tag="VFD89_05665"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZJ57713.1"
                     /translation="MLCNEKGYFGEFGGRFVPPELDKVLDEVGDAFERYREDPEFLAE
                     LRYYFQQYVGRPSPLYFAEKLTKKAGGAKIYLKREDLNHTGAHKINNTIGQVLLAKRL
                     GKKRVIAETGAGQHGVATATACALFDMECVVYMGEVDTQRQQLNVFRMELLGAKVISV
                     TDGLKTLKEAVDAALDDLIQNYENTYYMLGSAVGPHPYPAMVKAFQSVIGEEAKEQMM
                     EAEGCLPDYLIACVGGGSNAIGLFAPFYNDKDVKMIGVEPAGKGLSPGMHAASLTLGR
                     PGVIHGFKCYLLQDEKGEILPVHSISAGLDYPGVGPEHSYYKDSGRAEYVTITDQEAL
                     ESFFELSKLEGIIPALESAHAVAYATKLAGQLPPDQRIVVNLSGRGDKDVAQVSEIIK
                     EQQLVSDNRL"
     gene            16011..17012
                     /gene="floA"
                     /locus_tag="VFD89_05670"
     CDS             16011..17012
                     /gene="floA"
                     /locus_tag="VFD89_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837919.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HZJ57714.1"
                     /translation="MPDVAFYLLVLVAIIVFLSIFFTFVPVGLWISALAAGVKVGIFN
                     LVGMRLRRVVPSRIVSPMIKATKAGIDISINKLEAHYLAGGNVDRVINSLVAAQRADI
                     PLEFERAAAIDLAGRNVLEAVQMSVNPKVIETPNIAAVSKDGIEVIAKARVTVRANID
                     RLVGGAGEETIIARVGEGIVTTIGSSESHKNVLENPDKISETVLGKSLHAGTAFEILS
                     IDIADVDVGRNIGAQLQTDQAEADKRIAQAKAEERRAMAVAREQEMIAAVQEMRAKVV
                     EAEAEIPQAISSAFREGKLGVMDYYNMQNVISDTKMRESISGVSDSSSQTDDTPPIK"
     gene            17133..18854
                     /locus_tag="VFD89_05675"
     CDS             17133..18854
                     /locus_tag="VFD89_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57715.1"
                     /translation="MSTQDKGLSRRTHKREGPCLIAYLWAVPPMILLFLYRIVPFISG
                     LSLPFAQSASAMGTGPRIWVGMNNFRELFNSFSFSKVLGNTIILKLEYILLCSFLALL
                     LVLVLGSTQSKFWQRFFSAAFLFPYFIPTAVISYLILYTMGNAGMFFTFSMESMTDPR
                     IFRLIYPVLEVIKNLGIPIIIALGAINAKRESGQGTGGFIHMQLLPALKAIGLFVLIE
                     LSALLSSDFELLTIFHNPEAYGAADVIDTFTYRNGIVNMDTGVASSAWLVKFLIQTVI
                     SILIFLGIRKFSVQNIFTRHGSKNNKGGRLGSFVVVIAGLFILEIYLLLTAGPMAVSA
                     VETFKGSAGALNGLKDASSALIPALSYKSIIGHLLLISLAVVINAIVTILLTYPLTVK
                     DLPGRLLYSVSLIFILNIGVGGIHEFLFFKGRGIFGTVFPYIITGSFTLINVFVLRAV
                     YNARYKDAKEEAAKGAKGEMSSFFKGFLPRVIKPVLGLSALQYAIMWNSYYPRQLMYI
                     SDPAKFSPVMTFRNLVLGGQAEIYEFLTPEIIKLAAMVSIPGIIILLLLMIFGGEELF
                     ASQIRKS"
     gene            19373..19996
                     /gene="ytaF"
                     /locus_tag="VFD89_05680"
     CDS             19373..19996
                     /gene="ytaF"
                     /locus_tag="VFD89_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HZJ57716.1"
                     /translation="MLGSILLVLALSLDAFAASIAYGTNKIRIPLGSILVINFVCSLS
                     LGISLLIGSAVKMIVPGNILIVISFLMLMVLGVYYLFEGIIKASIGRSTGQNKRVKLR
                     LFDVKFIIEIYVDGTKADFNYSKSLSSREAIYLAMVLSLDSLAVGFGSSIVAINCWQV
                     LIFSLVFGIAAIWGGMYIGRRLAKKIEVDVSWIGGIILIILAFMKLI"
     gene            20150..22720
                     /locus_tag="VFD89_05685"
     CDS             20150..22720
                     /locus_tag="VFD89_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HZJ57717.1"
                     /translation="MTDISRMQKKNKKIKSNKHILKSIALVGLAIILVAGFFGAGAVK
                     SIIEDAPPLDIGVIEEQSQTSFFYDQDGEPITEYFGFENRVWATLDEIPVRLQQAFIA
                     IEDKRFKEHKGMDFIRLGGAFINNLKGGHVQGASTLTQQLVKTLYLTPERSYKRKIQE
                     AYLSIKLEKEFSKEKILETYLNTVNFEEGNYGVKAASKDYFGKEDMNDLTLKEIAVLA
                     GLVRNPASYNPRRNYHGGEDRRHITEDRAQLVLKAMLGNGYITEKEYNAAKDEELQVV
                     KEATRLKLYEMAPFIEYAIYEVRDAIIKLKGWQDDKSGLKKADEFIYSNGLKIYTTLD
                     RDIQKTVEDTVYNWDGYPDLEDDDNPLPQPQAAAVVMDNKGHLKAIVGGRTPPTNKRE
                     LNRAISPVMVGSTIKPVAVYGPALEEGKSPATIYHNIPVKIQGWNDQQDFPKNYGGGS
                     YSGPTTMRDGLIKSLNIVAAQALTYDVGFERSKVYLEGLGVDPSHIQANGSGLALGTS
                     SITPVELAGAYSAIASGGIFHEPISVLKVVDRDGKTIIDRTGNRLQRKVFSESTSWLL
                     VDMMKDAVKYGTGKNALIPEMTVAGKTGTNSDYKGVAFAGFTPYYTGVVWIGHDEGKP
                     LGSNVQGGKYAAPLWQAFMAKIHEDAGFEDRNILTMDPEGLGLVQKRVCKISGKLASP
                     DCPQDDVIVDWFDPKSVPEEVCDRHGTFEVCAESGKLPGPYCPEESISTRTAYFLEST
                     SPYWELSRDQIRKWIPKAHFEFSSLWEISGLDYSNPEHTKYFCDIHTEPPIPEAGGGL
                     DDAFDKLKDFFDGLFPGKNKGENQTQEDPDDGINEENVNGEDELHGDDVDDGHEDNDG
                     "
     gene            22968..24227
                     /locus_tag="VFD89_05690"
     CDS             22968..24227
                     /locus_tag="VFD89_05690"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZJ57718.1"
                     /translation="MGNGYNPYREMLEVLDRAAEKLGLEERDYIQLKYPERELKVSIP
                     VEMDDGSIEVFEGYRVQHSGVRGPYKGGIRYHQNVDMDEVKALAAWMSFKCAVADIPY
                     GGGKGGVTVDATKLSKRELERLTRKYATLLYPVVGPEIDIPAPDVNTNAEVMGWFMDT
                     YSTLRGHLSPGVVTGKPVPIGGSLGRGEATGRGVMLMVREISKKLHLPLEGARVAVQG
                     AGNVGGTAARLLHREGCKVVALSDVSGGILSEDGLDMDEIMDFLTAQRGRLLKDYDAP
                     NLVRISNEDLLLQDVDILIPAALENQITEEIAPHIKARIVVEGANGPTTVGGDRILEE
                     KGIVVVPDILANSGGVTVSYFEWVQNLQSFRWEEEEIRERLKNIMIKAFNEVWDTAVE
                     AETTLRMGAYMLAVDRIVTASKMRGLS"
     gene            complement(24480..25244)
                     /gene="srtB"
                     /locus_tag="VFD89_05695"
     CDS             complement(24480..25244)
                     /gene="srtB"
                     /locus_tag="VFD89_05695"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: protein motif:HMM:TIGR03064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HZJ57719.1"
                     /translation="MRRFILIVSIAVLLFSMYQIGEYLISARDTGNLYRDISGIYYEG
                     LADSGEDGEDKNSADTNDVKDHEKDAGGRSRINRLKDINPDIIGWITIPGTKIDYPIV
                     KGRDNSYYLNHDLLGNIDRHGAIFMDYRNDPPVDENLVIYGHHMRDGTMFKDLMKYKK
                     RSFYEDNGSIFLDMGGKTVEYEIFSAYISRADNVDLILAFGDKKGYEKYFSGAKKRSL
                     HPSDIDFADNNGMLTLATCTYEEKDARFVVHARTRE"
     gene            complement(25308..26882)
                     /locus_tag="VFD89_05700"
     CDS             complement(25308..26882)
                     /locus_tag="VFD89_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZJ57720.1"
                     /translation="MPITEMLSMNAQIYPNKTSLIERDPALGLRREITWSEFDNMSNR
                     LANALISHGIEKSDKVAILMMNCLEWLPIYFGILKTGALAIPLNFRFTGPEIKKCLET
                     AGAKILIFGPEFEDRIRGIAGKAKCLEHAFYVGDGQPSGMDGIANFFDIIRDVPTDAP
                     GIDISDDQEAALYFTSGTTGTPKPIVLTHSNLVSACITENHHHLQTHEDNFLCIPPLY
                     HTGAKMHWFGSLMVGSKAVILKGVKPEYILDAISEEQATIVWLLVPWAQDILEAIESN
                     QVDLDDFKLDQWRLMHIGAQPVPPSLIKRWRRHFPNQSYDTNYGLSESTGPGCVHLGI
                     ENIHKVGAIGRPGFNWQAKIVDKSGNALSYGQTGELLVKGPGVMKGYFNNEAETDKVL
                     RDGWLHTGDMARQDSEGFIYLVDRKKDIIISGGENIFPVEIEDFLQEHHDIKDAAVIG
                     MADERLGEIPVAIIEPKSGRRLGEAAILDFCRSLPRYKRPRKVIFDKVPRNPTGKIEK
                     PVLREKWCGKPSGVLV"
     gene            27514..28260
                     /gene="udp"
                     /locus_tag="VFD89_05705"
     CDS             27514..28260
                     /gene="udp"
                     /locus_tag="VFD89_05705"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HZJ57721.1"
                     /translation="MERQFHIDLDDGHGAEYAILPGDPERVEEIAGYLKNPEFLASKR
                     EYTSWIGELGGEKVIVTSTGIGGPSASIAIEELYRTGVRTFIRVGTCGGMQLDVVGGD
                     VVIATGSIRNEGTTREYMPIEFPAVADFHVVQALVQAAERLGYSYHTGVVQSKDSFYG
                     QHSPERMPAGYELLNKWDAWIRAGALASEMESAALFCVSSYLRARAGCVLNVIWNQER
                     GKAGMDNPRHHDNTRAIITAVEAIKILIER"
     gene            complement(28398..31928)
                     /gene="nifJ"
                     /locus_tag="VFD89_05710"
     CDS             complement(28398..31928)
                     /gene="nifJ"
                     /locus_tag="VFD89_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HZJ57722.1"
                     /translation="MGKKMRTMDGNTAASHVAYAFTDVAAIYPITPSSNMAEVVDEWS
                     AHGRKNIFGQTVRLSELQSEAGAAGAMHGALATGSLATTFTASQGLLLMIPNMYKMAG
                     ELLPGVMHVSARAIAAHALSIFGDHSDVMATRQTGFAQLASGSVQEVMDLAGIAHLAA
                     IKSRVPFLHFFDGFRTSHEYQKIEVLEYDDLASLVDYEAIDRFRKNSLSPESPVTRGT
                     AQNPDVFFQAREAANPFYNAVPDIVNEYMIKISELTGREYKPFNYYGAPDAEHIIVAM
                     GSVTQTIEETVDYLLAMGEKVGLIKVHLYRPFSEKYFLDVLPSTVKKIAVLDRTKEPG
                     AVGEPLYQDIKAILYGRENAPTITGGRYGLGSKDTTPTHIKAVFDDLKEADAKDGFTL
                     GIIDDVTNTSLIPSEYVNTSPKGTVRCKFWGLGSDGTVGANQNAIKIIGDNTDLYAQA
                     YFSYDSKKSGGVTVSHLRFGEQPIKSTYLIDEADFIACHNEAYVDQLDVIKGLAENGT
                     FLLNCTWSPEELDEKLPASIKRYLAKNNIDFYIIDALHIAEGVGLGGRINMIMQTAFF
                     KLANIIPVDEAIGYLKEAIQNSYGHRGEHIVKMNFDGVDQALAALVKVEVPASWADVK
                     DGKVVSDDDNKPAFIKNILEPIARQEGDDLPVSTFVGLEDGTMPAGTAAYEKRGIAAQ
                     VPQWQIANCIQCNQCSLVCPHAAIRPFVLDEDEMANAPEDFETKPAIGRTAKGMGYRI
                     QVSTLDCTGCGVCADVCPSRKKALVMKPLDTQIEKQKPLWEYAINEVSPKKDLMNKFT
                     LKGSQFQEPLFEFSGACAGCGETPYAKLLTQLFGDRMIIANATGCSSIWGGSYPASPY
                     TVNKEGCGPAWANSLFEDNAEYGFGMHLGVQQNRDRLAGLVDEAIDANIDPDLTQALN
                     KWLENKDLGEESQAATKELLPLLNKHNDNAVVKGILDLKQYLIKKSQWIFGGDGWAYD
                     IGFGGLDHVLASGDDVNVFVFDTEVYSNTGGQASKATPTAAVAKFAAAGKRIRKKDLG
                     RIAMTYGYVYVAQIGLGADMNHTVRVLREAEAYPGPSLIIAYSPCIAHGMRGGMVNSS
                     SRTKLAVQSGYWHLYRYNPLLKEEGKNPFILDSKEPTESFQDFLNTEVRYTTLAKTFP
                     EVAKEFFQVAENDAKDRYNIYKAMSESPIV"
BASE COUNT         9060 a         6434 c         8174 g         8594 t
ORIGIN      
        1 atataatata gtcggagtat tgctaacggg gttcataaat ggtttaaatc cctgttcttt
       61 ttcaatgctt ttgttcttac ttagcatatt ggtggcaaag aatgtaaaca tattgaagat
      121 tggcttcact tttattgtgg ggaaatttgt aacatacttt ttattgggta ctctgttatt
      181 taatattttt gtcagattag atatcccgca tttccaaata atgtcgaagg ttatcatggc
      241 tgtaatccta gtcttcatag ccattttaaa tttaagtgat tcgttcgctg cacgtaggga
      301 agattatgaa aggattagga tgcaattgcc tatctttcta cgcaaaaaaa atcaccagtg
      361 gataaataaa ttcggtaata caatagacca aacatttcta atgcctttaa gtctaatgct
      421 tggcgttttg gtttctcttg gagaattttt atgtaccgga cagatctacc tagctactat
      481 aatctcaatt ctccatgata gtaagacatt agattacagg gcgataatat atttcatttt
      541 atatggggtc tcctttgttt taccgctgat attagctact atatttgttt ataaaggcag
      601 gcagattttt gaactaaccg aatggctgcg ggggaaaatg cctgttgtaa aacttgtaaa
      661 tgcaataatt tttgtgttgt ttgctgtttt cattttaatt tggttatagg ggggactgga
      721 ttgagaaata ttacgatctc ttttgtggga aaaagcggta gtgttctttc gcataaaaaa
      781 ttacttgagc tgccattgag gaatgaattt attattggga ggagtattga atattttaat
      841 gatccggaac cttgtatgat tcatagatcc gctgtcataa aaagaatata tatggaatta
      901 ttcgattact tgggcggtat atacaccagc cgtgaggttg aaatgctctg ggatatcttg
      961 ccacaaaagt ttagaaaggc tatagatatc ccccaagagc cgcatttaat tcgcttttcc
     1021 tttcgggagg ataatagaag gaaacgataa attattattg ggttgtgcat tgttataaag
     1081 attttaatgc atttaatctc aacacatacg aacctacagc aatccgctaa tccaaatctt
     1141 atatatttta aaatgaattt gatggtggtt aatttgtcct taggggtata caatatatat
     1201 tagcatatta atgcatattg cagcggaact taagtattaa agcccagtac attatgataa
     1261 ctcaccatag tgatccaatg gtagtaaaag atgatactat atcccctatt atgttattat
     1321 tcttggtcat ctaaaatatt gggcgccata gttattagtg ggagattcgg gtgggggtag
     1381 attacacaga tagcgtgtaa aataatgttt tagaaagaga aaggatgata ttctattatg
     1441 tccaaggaac aaaagagcag acgcattggt atcggatcta ttggtttggg tgggatttcc
     1501 aagggtgttc acctccccgg tatagaaagg agcgaggact ttaagctggt ggctctatgc
     1561 gacatagatc ctgaggcctt aaaggcggca caggaaaagt ataatatacc ggattcccgc
     1621 tgctttacca attatcagga tcttattgaa tgtcccgacg tcgaggcggt ggacatatct
     1681 acgcctaacg acagccactt tgaaatcgcc tatgccgcag ctgtggcggg taaaccctat
     1741 gcgctggaga agccgataac tatgaccaaa gaagaggccg acaaattggc tgagataacc
     1801 gaagagaagg gcctaaagag catggtatgt ttctcctatc gcttcatacc cgctgtcagg
     1861 tatatgaagg acataatcga caggggctta atcggtgata tctaccacgt aaatatgcag
     1921 tattttcagg cttggggcct tcccgatgcc aattgccccc tcgtatggcg ctacataaag
     1981 gataggacag gttccggtgc gctgggtgat ctgggctctc atggtataga cctggtccgc
     2041 ttcgtaacga gcaaggaata taaaaaggta ataggccatg cagatacctt cgtaaaggaa
     2101 agacaggtgc cgggcagcga tgatatgggc aaggttgatg ttgatgactt ttgtaactat
     2161 atggccgaga tggacgggag aacatctgcg aacttccaga taactcgctt tgcgtacgga
     2221 agggggaatt accagaggat ggagatatac ggaagcaagg gagccatggt atatcacctg
     2281 gatagatcac ctaatgccaa tgaaatcgaa gtctgcatag gggagataaa tcgtgaagcg
     2341 cggttgttta cgaagctgcc tgtaccggat aagttcaagg ccgatcagat gcaatccttt
     2401 gccgatatca tcaacgacaa gggcgacgga ttagctgcca ctatatttga cggccagtcc
     2461 aatcagcatg tactggacag catcattgaa tccgtcgaaa agggcgaatg ggtgacatta
     2521 taggacgatt atgcttaagg caatagtgaa gtgtatccta attttgggat aaaaaccgaa
     2581 aaaatgaaaa ggagcgtttt aaatgggtaa gattaaggta acggtatgga acgaatatct
     2641 tcatgagttg caggttccag agattgggga aatatatccc gatggtatcc ataattgcat
     2701 aggcgattcc ttaaaggatg cgggtatgga cgtaaaaacc gccactttga gaatgccgga
     2761 gcacggcctg accgatgaag tgttggatga taccgatgta ttggtctggt ggggacatat
     2821 ggcccatggc gaggtggaag atgagatagt tgaaaaggtt tatgagaagg tcttaggcgg
     2881 aatgggcctt atagtccttc attccggtca tgcatctaaa atcttcaaca aagtctgcgg
     2941 aactgccacc ggaatgctga aatggagaga ggacggggat aaggaaattc tttgggtagt
     3001 cgaccccagc catcccatcg tagacggatt ggacgagaag ataatcatac ccaacgagga
     3061 gacctacggc gagcatttta acatcccaaa tcccgatgat ctggtgtata tcagttggtt
     3121 tgaaggcggc gaggtcttca ggagcggatg ttgttactac agaggtaagg gtaaggtatt
     3181 ctactttaga cccggacatg aaaccttccc tatctatcat atgcctgaaa ttcaaaaggt
     3241 tatagctaac gcggtcaaat gggctgcccc tataaatttc ccggatttaa cctatggaaa
     3301 caccaaacct gtggtagaaa taaagaaata gggaaatggt gtagaaataa agagataaaa
     3361 aaatatctaa ataaaaacat gatgttttcc aggatttagt tttatgaacc tgggaaggca
     3421 aagttttata aggttgtcat ccttcgtctg cgggggatga caaccttact attgtgatat
     3481 tttccttccc aacaggttga ttccccacat ctattgatta agttcctgaa gtattcacat
     3541 gggattttta acgattggaa aagtatggtc caatatgcgt ctgcttttta ttcacaaaaa
     3601 tgattatttg gggtataata tggttattat tcagaaatcc gattggaaaa gacatatata
     3661 gtacttttat tggcttatat agcctttggt ttggattata tgtaattgtc tggctatgat
     3721 tactttgaca aagggtgatt catgacaaaa tcatgttcgg gactttgtgt ctattctaca
     3781 tagcccaaat aagcaggata acaggggcga aaggagcagg ataaaatgaa ggatattatc
     3841 ataataggcg gcggtattgc ggggttgacg gcgggcatgt atgccaaacg agccgggatg
     3901 gagaccatat tgtttgaaaa attgtttccg ggaggtcagg ccgcgaccac atttagtatc
     3961 gaaaattacc cgggatttgt cgagcccatc agcggtccgg atttttctat gaaattagaa
     4021 gagcacgcgc gaaagtttgg attggaaatc ctctacgatg gggtagatga gatcattatt
     4081 gacggaaata tcaaaaaggt ggtaaccgat ggcgggacct acgagaccaa gaccatctta
     4141 ttagcaacag gggccagccc caaggaactg ggcctggaaa aggaagaaca attcaggggc
     4201 aggggggttt cctattgtgc tacctgtgac ggtaccttct acaaagataa ggttactgcc
     4261 gtcgtagggg gtggggacac cgcattggag gatgcttttt ttcttgcgca atatgcatcc
     4321 aaggtatatt tgatccaccg aagagatgaa tttaggggcc ttaagatact ccaggacagg
     4381 gtgcttaaac atgaaaagat tgaagtcctt tgggataccg tagtggaatc cataaccggg
     4441 gaagaacagg tggaggggat cggggtaaga aatgtaaaga ccggccaaac taccgagctc
     4501 agcctggatg gcttctttat agccattgga attacaccca atagcgagct ggcaaagggc
     4561 aaactaaaca tgacgccgga cggttttatt attggagatg aatccatgaa tacaaacgta
     4621 tcgggagtgt ttgcggtggg cgacgtgcgc tacaagccgg tatggcagct ggtaaccgcg
     4681 gcctctgatg gggcggtagg taccctgtca gcccaaagat atattatgga acattttgaa
     4741 taggcattat agctgttttt atacaacaac cggcgttata gaatcatatt atggcattgt
     4801 gggttatccg gtgagataat atgattttta aacgtcctta atgattaaga gggtgacatc
     4861 atgggtcgat tatttgggac tgacggggtc aggggtttag caaacgagaa gctaaattgt
     4921 gagttggtat acaagttggg ccaggctagt gccagggtat taaccctggc aaaatacagg
     4981 cctagaatat tggtgggcag ggataccagg ttatccggcg atatgctgga ggcagccttg
     5041 gtctccggta tttgttccgt aggctcggag gccagggtag taggggtgat gcccactccc
     5101 gctgtagcct atctagccag gcattataag gcggatgcag ggattgtgat atctgcgtcc
     5161 cataattcta tggaatataa cggaataaag atatttaaca accagggata taagctatcc
     5221 gacgaactgg aagaaaagat cgaaagcatg atattggata acacggagag catcacctac
     5281 ccaacgggta ttaacgtggg gcgcatagtt cgggttaaaa atgcatcaag acattatgag
     5341 agctttttaa aagggaccat agatatcagt ttggaggggc ttaaactggt ggtggactgc
     5401 gccaacggtg catcctccaa tatagcgcca aggatatttt cggagctggg agccagggtc
     5461 cataccatat atgatgatcc tgatgggata aacatcaaca acctctgcgg ttcaacctat
     5521 cccgataagc tccaagatca tgtaatagaa accggcgccg atttaggcct agcttttgac
     5581 ggtgatgctg acagattggt agcggtagat gaaaatgggg aattggtaga cggtgatcag
     5641 ctgctggtga tctgcggctt gaacatgaag aagaggggcc tgcttaaaaa ggacacggtg
     5701 gtgggcacca ttatgagcaa tctggggttt gatgaggctc taaaaagcgc gggatgtagg
     5761 gttgtaaaga ccaatgtcgg ggaccggttt gtcctagaaa ggatgctgga ggagggatat
     5821 aacctgggag gggaacaatc cgggcatgtc attttcttag attataatac tacaggagac
     5881 ggaattttaa ccgcacttca gcttttggcg atattaaagc gggagaacaa accactttcc
     5941 caattggctt ccgctatgga aaagtatccc caggtgttaa ttaacgccag ggtcgatgaa
     6001 atccaaaaaa gaaaatatac cgatgatccg gagattcaaa aggaaataaa agccgtagag
     6061 gacaggttca agggcagggg cagggtgctc attcgccctt ccggtacgga accgttgatc
     6121 agggtcatga tagagggcaa ggatcaaagg gaaatagaag aacatgcggt cgctctggca
     6181 gggttaatcg aggaacggtt aaagtaatat gtgggttttt gaaattaaaa catggcagca
     6241 gacttctaaa acaagggttt gctgcttttt cattccatcg aatggaattg tttccggtgg
     6301 gttatccata atggtggttt acttttgact tatgcctact atataattga actataatca
     6361 tgggttagat agaggtcggg aaaaggacct tccataatac ctatagatcc cctatgcggc
     6421 aaaaattaat ttatgggata tgaacatttt cgcataaaag gcagggctgc atagggcgca
     6481 cgaaaaataa cggaaggaat tgatggaatg tataacagga ggattttagc ctttgctttg
     6541 attctggtaa tggctgtact aatgggatgc gcatctaagg cgggaaatga tgatttaaag
     6601 gacagaaccg tagttgggaa ggtcaacggt gaaaaaatta ctaaaggaga attcaacagc
     6661 ctttataacc agatgaagca aaactactat atatcggaag aagttgaaca ggatccggat
     6721 cagagtgaaa tgatagccga attaaagctg gatatattgg atcagctggt gatagaaaaa
     6781 ttgatggccc agaaagcaaa gggggcgggc tttgaggtaa ccgatgatat attggaggaa
     6841 gcccgtgcag aatttgagga gattgttatc cacctggcag ggcagatgga gataatagat
     6901 ggcgatacac aggatgaaca ggggcaggag acggatgata caggcaaaga ctatatagag
     6961 gaagccaagg cctatataga acaggaactg gctgccatgg gacaaagcca ggatgatttc
     7021 attgattctc tagctaagca gatgaccgta gacggatata tagagtccct gctggaggat
     7081 ataagcgcag ataaagatga aatagaggag tattacaggg agagacttca gaaccaaaaa
     7141 tcgaacattg ccggcataaa ctatgaggaa gtagagctat ataagcctga ggaagtcagg
     7201 gtaaaacatg ttctgatcgg gctgccaaaa gaggaactgg aaagatatga tgagctcatg
     7261 ggtgacggta aagagaagga agcaaaaaaa cacctggaag ataagctgaa ggaaatcgaa
     7321 ccgaaggcca tggaggtact gcaaaaggcc cgggacggag aagacttcga aaaacttatt
     7381 gaggaatacg gggaagaccc tgggatggag gacaatactc cgggctatat agtaagacag
     7441 gacggggatt tcctgccgga atttgaaaag gcggctttta ctttggagga gggggagata
     7501 tccgatttgg tggccacgga cttcggttat cacatcataa aattctatga aaggtatccc
     7561 caaaaggtat atactctgga ggaaaagcac gacgaggtca aggagttttt agactatgag
     7621 aaaaaggctg atgcctggac caccatcttg gatgaatgga tggcggatgc cgatataaag
     7681 agatacgaaa acaagctgta acagtgggag agccggctga tattaattct gtgggattgg
     7741 gagttataag agtgaagagg aaagtgttaa tagtagacga tgagagctcg attagaagct
     7801 ttataagggt tgggttcgaa aggagcaact ttataataat cgaagccgaa accggggaag
     7861 acggtataga gttagcccgg gagcatatgc cggacatcgt gatcttggat attatgttgc
     7921 ccggcatgga tgggtttgaa gtgtgtattg ctctgcgttc cgaatttccc gatatgggga
     7981 taataatgct tacggccagg gacctcgata tggacaggat aatggggttg gagcggggag
     8041 cggatgatta tgtagtaaag ccctttaatc ctttggaact ggtcttgagg gcggaggcgc
     8101 ttctcagaag gatcggtaca agggacggtc agggggatga tcgcagattg tgggacgagc
     8161 cctttgagat agacacctat tcccaaaagg tttataagag ggacttggaa atagacctga
     8221 ctcccaagga atatcaatta atgaccttat ttgtgaagaa tcccggaagg cttttcagca
     8281 gaaacgaact catagacacc ctatgggggt cggactttat gggggatcta aagatagtgg
     8341 atgtcaatat ccgaaggctc agaaagagga ttgaagataa tccctcccat cctatgttta
     8401 ttgaaacggt gtggggagtc ggttacaggt ggagggaaaa gtgagaaagc gaaggaagag
     8461 cataaggacc agattgagct acagctttat gagcataatc ataatcagca tctttatatt
     8521 tatctttatg gtcagcctat ttttaaagca atactactac aagggggtcg aggacatttt
     8581 gacagaccag gtcaatatgt ccgttaattt atattcccaa tactttattg acagctccct
     8641 tcaggataat gttatggata atgtggatgc attttggagg ttttcatcgg cccaagtcca
     8701 aattatcgat aataggggaa gagtgctgat ggattctatc ggtatggatt ttaccgatga
     8761 attggacacc cccgatgtca aaagggctat gatgggggaa tggggtaaat ggatcggcag
     8821 gatgccctat acaggggaga tggctatgtc ggtagctcag ccacttaagt caaacggcga
     8881 tattataggg gtcctgaggt ttataacctc cctgactcca ataaacggcc agcttgaggg
     8941 gatcataagg gtctttatgc tcatagggat tttagccgtc ggtatatccg gaatcatcag
     9001 ctttgtttta gccaatgggg tggtgggccc tattaagcag gttaccaaag tggccgaaga
     9061 gatggcaaag ggagactttg atgtaaggag tacggaaaaa acccacgatg aaatcggacg
     9121 tttatcgtct actctaaact atatggcaga tgagataaca aagaaggaac gggtaaagaa
     9181 tgaattcttg gcctcggtat cccatgagct tagaacaccc cttacatcca ttaagggttg
     9241 ggctatcatt ttaaatgata cggagaataa agaagagaag atctttaggg acggcctgaa
     9301 aataatcgag aaggagaccg aaaggttgtc ggggatggtt gaggagctat tggatttttc
     9361 caagctgttg tcggggaata tctccttaaa attggtagag acggatatag aaaggcttat
     9421 agagtatatc aaacagcata tgatgcccat agccaaaaga aataagatca ccttccgtgt
     9481 agagtgtccg gataaactcc cctgcatagt cgtagattcc aatcgcataa agcaggtgct
     9541 tatcaacgta ctggataatg cctttaagtt tacctcccaa gggggtaatg ttaggcttgc
     9601 ggcctatggt gcgggggacg atctagtcat ggagatagag gatgacggat gcgggatatc
     9661 ccccgaggaa ttgcccagtg taaaggaaaa attctataag ggaaaaaccg gaaagtcatc
     9721 aagcggtata ggcctgtctg tagcctatga gatagtaaag aagcatcagg gcgggctcga
     9781 catagagagc agaccgggta tggggacaag ggtctatata agattgccta ttgcacacgg
     9841 gacaggccgc aactagaggg ggatcgggtt ttgaggttta taaatgtaag cagcattata
     9901 ctcatatgta ttttgttttt cgccggatgt cagggtattg agaccttaaa ttcggcccag
     9961 accattattc cgcctgaaaa caatagaatt tcgattcagg ggacttggaa aatcaaggat
    10021 tataaaagga tgtccgattt gcaggaagaa aatgatgccg tcgccggcag tgaggagctg
    10081 ctgggcgaaa tcgccttatt tgctcacgat tgggctgtgg tagtcgatga gagttgtacc
    10141 gaggcccaat atcaggtgag gcgggtaaat gccgtggatt attttctgtt ccatcataat
    10201 acagacgtcc aagagttggg tgtggagaat gaaatgatag agatcatatc cgtaacgtcc
    10261 aatggtgtct tgttcttcga tattctaaag atggatgatg ataccgccat tatatatatg
    10321 gataagtgct tttattggct ggagaaggta tccgacaata tagagagatc ccgaaatgag
    10381 tatgggaccg gtacaaataa agaagatgtc aacaggtatg aggaagaaaa gattgtaaga
    10441 tccggggtat ttttaggtct gcgcactacc atcggccccg gcgatgatgg atatggcaac
    10501 cgctctgcct atagaaccat atggatttca tccaacaata ggaggataga gtccgtgctg
    10561 gagacgtcca atcttttcat accaaggcga agcggatttt ggaagttgga tgtgggaacc
    10621 aggcgaatag gcgggttttt gcaggattat ttcgacctta gtccggtgga ttctatggga
    10681 gtcagccctg gggaatctgg caatgccgga gagctgctgt cctttgtagg taagaatctt
    10741 aaaaagaata tactttttat aagcgacgat tatatcgcta tagagtattc ggagacggcg
    10801 aaggatgaaa taagggagcc agacaagtat atggtcttgc ccctagacga cgccaacagc
    10861 caaaaaggca tcaaaatatc caactttgcc gatgagggca tggaggatat atttcataag
    10921 tccgcccaat ccttcttggt atcaaagggt aggaaaatga cgggcgattt aagagaatca
    10981 gccggggaag aaaattttac tgtagcaagg cgaaacggac attggattct taggggcagg
    11041 ctgaatattg acggaatcga cgaggatttc aatatagggc tcatgcccgc cggaaaactt
    11101 ttaaactatg ataagctcca tgtgtcctgg gacgacatca agggaaaaat acctatggcc
    11161 ctcgatgcct atacgtctcc tgccaaggat atactaattg cagttacggg caacttttta
    11221 atggtgtata ccatagaaga tggacggata tccgaaaagc caataaagaa gataggtatc
    11281 aaaaaagggg agacggtgat catggcggaa tgggctacag gggattacgt agcccggtgg
    11341 gagaagggtt ttaaccagat taatcccttt gtggttaatg aataaacaca aagtggttaa
    11401 actaaatttg gagaataata ttcaaatcac taaaggtcca aggccaagaa cctgtagact
    11461 tgctgcaggg cctaagcctt tacacaatgt ggtttttata tagttctcca aataaaaaga
    11521 tatgccatat aattaacgac ttgccttatt ctgtaaatgt atcccgcaac agctccggta
    11581 tggtaactat atcatagccc tgaccttgta tatactctat aatttttggc agcgccttta
    11641 tgctattggg gatggatttt tcagaaccgc tggagtgcaa taaaattatg gcaccgggct
    11701 ttaaattttt cttcacatca ttcaatatcc tattgggcga gattcccgac caatcccttg
    11761 ggtcgatgga ccaatatatg ttatgaaaac cctcgtctgc taccgtcttt aaaacctttt
    11821 tattaaaggc cccgtagggc agccgaaaca gcgtattgct tttcccggtg acttcctcta
    11881 taagttcatt ggttctttta agttcttgga tagccttgtt cttggaaagt ttggtgaagt
    11941 ttttgtgtcc ccatccatgg ctggctacga cgtggccttc atcatcaatc cttttaagta
    12001 tatcccggtt gttttgggca acggagccca gaatgaagaa ggtggatctg atatcatatt
    12061 cctttagtat atccaatact tggggcgtaa aataatcatc gggcccatcg tcaaaggtga
    12121 tggcaacctg ttttagctca tcgttggaag aatttcgtat gatctttgcc gcaggaacgg
    12181 cgtctatatc gtcaaatccc gaagattcct caatctgctg ctttatatcc tcgtctgggc
    12241 catggggatt tccatctccg tctatatcca caggcttttt attatcaccc tcggcattgc
    12301 cgatattccc atcagccggt gccgttatat tattgtttat cgtttcatcg gactcgccgt
    12361 ccaggtcttt tacgttacct attcctgtat ccttatgggc gtgtatatta tcataagggg
    12421 ctgtattatc ctgatcccgg ctatcgtccg atgaagacgg cccgttgtcc ccatcccctt
    12481 tagcgtccga tgaagacggt tcagtatccg catctccttt atcctccgtt gtttccgggg
    12541 cctgtcctgc cccattgtta tcctcccaag aggacggagg tccatcagcg ggcttattgg
    12601 caggcgcgcc gggagaagac ggtttcagag aaaatatatt gatatatcta aagctgcatg
    12661 acgacaaaaa gatacatagg agaataattg agaaaacttg aatcgttttt gaaaaactgc
    12721 ccttgcccat ttttttcata gcggataccc ccctacacat ttctacattt aagacccttt
    12781 accttaattt cgggatcatg atatgataat aaatatttgc tacactccaa ttgtatgcaa
    12841 taatataatg gaaagagtta caaaaaggtt acaaggggga gtaaataata ttaactttcg
    12901 aatcttgtag aaatatatat tatttaccct atcgtaacgg ccccctatgt gattatatat
    12961 gcaatacgct ttcgtatata atggtcccgg gtcctcttaa aatcggtatt taaatgggaa
    13021 ttcaatgggc accatgcgtc tttttgattg gtataatgca ttaatttagg gttttgatca
    13081 aatggataaa aaactctatg cttaatactt ctaattgtgt atacttgagg tagaagataa
    13141 tacgacttgt cgtttaattt acaaagacaa aatttgttct attggaggta tattgatgga
    13201 aaaattaaca acctatgaac ggatcaaaat gatgtatgaa catagggatg ccgatagggt
    13261 gcctatattc gattcgcctt ggacggctac tataaagaga tggcacgagg aaggtatgcc
    13321 tgaaggtatg gattacaggg attttttcca gctggatcat atagccacca tagaagtgga
    13381 taatagcttt aggtatccgt ataaattgat agaggaaacc gatgattata taacatatac
    13441 gtccaaatgg ggggtaacgc aaagggaatg gaaaaatgcc aactccacac cggaattttt
    13501 ggaacatacg gtggtggacg aggagtcctg gctaaaggta agggataaga tgacccctga
    13561 tcgtgacaga ataaattgga aacaccttga atccaactac aagaaatggc gagagcaggg
    13621 atggtggata gaggggcttt tatggttcgg ttttgatgtc acccattcat ggatggtagg
    13681 tacggaaagg ctcctctttg ccctactgga aaatcctgaa tggtgtatgg atatgttcaa
    13741 tcattttctc gatgtgaata ttggactcct ggatatggtg tgggatgcag gctataaatt
    13801 tgatagcgtg atctggtacg acgatatggg ttacaagcat aaccaattct tctccttaaa
    13861 gacctacagg gaagtattaa aaccggtaca caaaaaggcc atagattggg cccatagtaa
    13921 aggcgttaag gtaaggttgc actcttgcgg cgatatcaat cccctcttgc cggatcttgt
    13981 ggagatgggt attgatgccc taaaccctat agaggtgaag gcaggaatgg acccagtagg
    14041 attaaagtca aaatacgggg ataaattggt cctccacggt ggagtggacg cgcttttatg
    14101 ggaggatata gacaagatgg aaaaggaact gaggaggata cttcccgccc taaaggaaaa
    14161 tggcggctat atattttcta cggatcattc cataccatcc aacgtgagcc tggatcaatt
    14221 taggtatatt acggatttaa tcaaggatat cggcaggtat tgatagtacc tatatgtttt
    14281 acggcaaaca tcctacatta gtatagatta gaaaatggag cgtagacgag agatttgata
    14341 tggctatata ctagggggaa cgattttggc agcagtcatt cagctctaca gacatgggga
    14401 tccgtcccga agtatagatg gtcttttaaa aaactgcggg tatggtcaat gactatatat
    14461 cccattagaa aattataggc attgacatta tcggaaattt cgtatatatt ttattaaagc
    14521 ctaaggttgt attatacagc ctgcttggct aagatgagaa gagtcgagat tagaagagag
    14581 gagaatagct atgttatgta atgaaaaggg ttattttggt gaatttgggg gacgattcgt
    14641 tccgccggag ctggacaagg tattggatga ggtaggggat gcttttgaaa ggtataggga
    14701 agacccggaa tttttagccg aacttaggta ctactttcag caatatgtcg ggagacccag
    14761 cccactttac tttgccgaga aacttacaaa gaaagccggc ggtgcaaaga tctacctcaa
    14821 acgcgaagat ttaaaccaca cgggggccca taagatcaac aacaccatag gacaagtact
    14881 ccttgcgaag cgtttgggca aaaaaagggt tatagctgaa acaggagcag ggcaacatgg
    14941 ggttgctacg gccacggctt gcgccttgtt cgacatggaa tgcgttgtct acatgggcga
    15001 ggtggatacc caaaggcagc agcttaatgt ctttaggatg gaattgttgg gtgccaaggt
    15061 catttcagtc accgacggcc tgaagaccct aaaagaggcg gtggacgcag ccttagacga
    15121 tctgatccaa aattatgaga atacctatta tatgctgggt tcagcagtcg gtcctcatcc
    15181 ctatcccgcc atggttaagg cgtttcaatc tgtcattggc gaagaggcca aggaacagat
    15241 gatggaggcc gagggatgtc tgccggatta tttaatagcc tgcgtaggcg ggggcagcaa
    15301 tgccataggg ttgtttgcac cattttataa tgataaagac gtgaagatga taggggtaga
    15361 acctgcaggc aagggtctta gtcccggcat gcatgccgca tctttgactc ttggaagacc
    15421 tggggttatc catggtttca agtgttatct gctccaggat gaaaagggtg aaatccttcc
    15481 ggtgcactct atatcggcgg ggcttgatta tccaggggta ggtccggaac atagttacta
    15541 taaggattcc ggaagggcgg aatatgtaac cattacagat caggaagcgt tggaatcctt
    15601 ctttgagctt tcaaagcttg aggggattat cccggcccta gagagcgccc atgcggtagc
    15661 ctatgccacg aagctggccg ggcagctccc accggatcaa agaatagtgg tcaatctatc
    15721 cgggcgagga gataaagatg tagcccaagt atctgagata atcaaggaac aacaattggt
    15781 tagtgataat agattataga acaaaatata attccatgat taaacaataa tcaatataga
    15841 ggcaggcctg ctcttgaggc ctgctttttg tataaaaatt caaaaaattg tttccgaata
    15901 actggtataa tcccatggat tgctatataa taaacatata tcgtgctttc agcatcgggg
    15961 ctgtcgcccc ggtgacttta aaagaattat tatagaaagg agttttataa atgccggatg
    16021 ttgcatttta tctattggtt ttggtggcca taatcgtatt tctatccata ttctttacct
    16081 ttgttcctgt cggcttatgg atctctgccc ttgccgcagg tgtcaaggtc gggatcttca
    16141 acctggtcgg aatgaggctt aggagggtag tcccgtccag aatagtcagc cccatgataa
    16201 aggccactaa ggccgggatc gatatttcca tcaacaaact tgaggcccat tacttggccg
    16261 ggggtaatgt tgacagggtc attaattcac ttgtcgctgc ccaaagagct gatattcctc
    16321 tggaatttga gcgagccgca gccattgatc tggccggaag aaacgtttta gaggcggtcc
    16381 agatgagcgt taatcctaaa gttattgaga cccctaacat cgcagctgtt tccaaagacg
    16441 gaatcgaagt tatcgcaaaa gcccgcgtaa ccgtaagggc aaatattgac agactggtgg
    16501 gcggagccgg tgaagagacc ataatcgcca gggttggtga aggtatcgtt accaccatcg
    16561 gttcttccga atcccataag aacgtgctgg aaaacccgga caagatttcc gaaaccgttc
    16621 ttggaaagag cctccatgcg ggcaccgcct ttgaaattct atccatcgat attgccgatg
    16681 tggatgttgg aaggaacata ggcgcccagc ttcagaccga ccaagccgaa gccgataagc
    16741 gtattgccca ggcaaaggct gaggaaagaa gggccatggc ggtggccagg gaacaggaga
    16801 tgattgccgc agtccaggag atgagagcta aggtagtgga ggcggaagcc gagattcccc
    16861 aggctatatc atccgccttt agagaaggta aattaggggt tatggattat tacaatatgc
    16921 aaaacgtcat ctctgatacc aagatgagag agtccatatc gggggtaagc gattcatcgt
    16981 ctcagacgga tgatacgcct cccataaaat aacaccatgc tggtttggca cacatcggta
    17041 atgtccattg aacactgact attaaaggag ttgtcccata aggagcagaa tgctctttgt
    17101 gggacaacaa gtatataggg ggtaaaaaaa taatgtctac acaagataaa ggcttgtccc
    17161 ggcgtacaca taagagggaa gggccctgcc tgatagcata tctatgggct gttccgccga
    17221 tgattctact atttttatat aggattgttc ctttcatttc cggcctatcc ctgccctttg
    17281 cgcaatccgc ctcggccatg ggtaccggcc caaggatctg ggtggggatg aacaatttcc
    17341 gggaactatt taattcattc tccttttcta aggttttagg caataccata atcttaaagc
    17401 ttgaatacat attgttatgc tccttcttgg ccttactcct agtcctcgtc ttgggctcaa
    17461 ctcaatcaaa attctggcag aggttctttt cagccgcttt cctgtttccc tatttcatac
    17521 cgaccgcagt aatcagttat ttaattttat acaccatggg taatgcgggt atgtttttta
    17581 ccttttctat ggaatccatg acggatccaa ggatctttag gcttatatat cctgtcttag
    17641 aggttataaa gaatttgggg atccccatta tcattgcatt gggggccatc aacgctaagc
    17701 gagaatcggg gcaggggaca ggcggattta tacatatgca attattaccg gccctaaagg
    17761 ccataggatt atttgttctt attgagctat cagccctgtt gagctcggac tttgaattgc
    17821 ttaccatatt tcataacccc gaagcctatg gtgcggcaga tgtcatagat acctttacct
    17881 ataggaatgg aattgtaaat atggataccg gtgttgccag ctccgcctgg ctcgttaaat
    17941 ttcttatcca aacggtcata tccattctca tatttttagg tattagaaag ttttctgtac
    18001 agaatatatt tacacgtcac gggtctaaaa acaataaggg cggcaggctg ggttcgtttg
    18061 tggtggtgat agccggattg ttcatcctgg aaatctattt gttactgacg gcggggccca
    18121 tggcagtgtc agcggtagag acctttaagg gcagcgccgg ggcgttaaat ggcctcaagg
    18181 atgcatcatc cgcccttatc cctgccctat cttataagtc catcattgga cacctgctgc
    18241 tcataagtct ggcagtcgtg ataaacgcca tagtcacaat tcttctgacc tatcccttga
    18301 ccgtcaagga tctgccgggc agacttttat attccgtatc tttgattttt atcctcaaca
    18361 taggggtggg tggcatccac gaatttctgt tctttaaggg cagagggatt ttcggcacag
    18421 ttttccccta cataatcacc ggttcattta ccttaattaa cgtgttcgta ttaagggccg
    18481 tctacaacgc caggtataag gatgcgaaag aagaggcggc caagggagca aaaggggaaa
    18541 tgtcctcctt tttcaagggg tttttgccca gggttattaa acccgtgttg gggctttcgg
    18601 cgctgcaata tgcaatcatg tggaactcat actatccgag acagctgatg tatatatcag
    18661 acccggcaaa gttttctccg gtaatgactt ttcggaatct ggttctaggc ggacaggccg
    18721 aaatatatga attcttgacc ccggagatca ttaaactggc tgcaatggtt tccataccgg
    18781 ggattatcat cctgcttttg ctgatgatat tcggtgggga agaattgttt gcaagccaga
    18841 taagaaaatc ttaaaaaaat gggggtggcc aagggccacc cttttgatta acctgtcgtg
    18901 ttgcttcatt aactgggtaa tagactattg aaaagctaat gacttcattg attgggcttc
    18961 tagaaattag aagttggaag ttcgaaacaa aacaagcagg acgccttaat ccgattccgc
    19021 cagcaggtca gttaatcaca tagacatgtc cggcagcaac gccaatacat ccagtaacca
    19081 atagcgcata gatgaataac atattactaa tggagtctcg gaaaacacat ggtttcatgg
    19141 gcttggtcgc gaccatggtg atgaaaattt cagaaactcc gattgattag ttgttttagg
    19201 taataactat atgttttacg acagacattc tacgctggta cacaggtgtc gtaaaatatt
    19261 tccttaattt gcaacacaac taatcaaata aatgagggct tcattgtttt tttaacatcg
    19321 aagcctttgc ccgattgggc ccaaaggatt gggcggaata aggggtggta agatgttagg
    19381 ttctattctc ttggtattgg cattatccct ggatgcgttt gctgccagta tcgcatatgg
    19441 taccaataaa atcagaatac cattaggatc cattctggtt atcaattttg tatgttcttt
    19501 gtccctgggg atatcgcttt tgatcggttc ggcggtcaaa atgatcgtac ccgggaatat
    19561 cctaatcgtt ataagctttt taatgctaat ggtgcttggt gtatattacc tctttgaagg
    19621 aattataaag gcaagcatag gaaggagtac aggccaaaat aaaagggtga aactcaggct
    19681 ctttgatgta aagtttatta tagagatcta tgtggatgga actaaggcgg attttaacta
    19741 ttccaagagc ctcagctcaa gggaggctat atatttggcc atggtcttat ccctggattc
    19801 cctggctgtg ggattcggaa gcagtatagt agccatcaat tgctggcagg tgctgatatt
    19861 ctcattagta ttcgggatcg cagccatatg gggcggaatg tatatcggca gaagactggc
    19921 caagaaaatc gaggtggatg tgtcttggat aggcggaatt atcctaatta ttctggcatt
    19981 tatgaagctg atttgacacc ctatttggcg atccccgttt agaggtaaat cctaattcaa
    20041 catatccata taccggcgca attgcttaca atgttttatt caattaataa attgtttata
    20101 gattggacat attttcgtgt tagaattgtg ggtatgaggt gatataggta tgactgatat
    20161 atcgagaatg cagaaaaaga ataagaaaat aaagtccaac aaacatatat taaaatccat
    20221 agccctagta gggttggcta tcattttggt ggccggattt tttggtgcag gagcggtgaa
    20281 atccatcatc gaggacgccc cgccactgga tataggggta atagaggagc agagccagac
    20341 ctccttcttc tatgaccagg atggcgaacc cataaccgaa tatttcggct ttgaaaaccg
    20401 ggtctgggca accttggatg agattcccgt aaggctccaa caggcgttta tcgccatcga
    20461 ggacaagcgg tttaaagagc ataagggtat ggattttatc aggctcggcg gtgccttcat
    20521 aaacaacctc aaggggggcc atgtgcaagg ggccagcacc ctgacacaac aattggtaaa
    20581 aaccctctat ctgaccccgg aacggagtta taagaggaag attcaggaag cctacctatc
    20641 cataaaattg gaaaaggaat tctccaagga aaagatactg gaaacctatc taaacacggt
    20701 gaatttcgaa gaggggaact acggcgttaa ggccgcttcc aaggactatt ttgggaagga
    20761 ggacatgaac gatctgaccc tcaaggagat tgcggtattg gccggactgg taagaaatcc
    20821 ggcatcctat aatccccgga ggaattatca tgggggcgag gacagaaggc atataacaga
    20881 ggatcgtgcc caactggtct taaaggcaat gttgggaaac ggctatataa ccgagaagga
    20941 atataacgct gcgaaggatg aggaactaca ggtagtaaag gaggccacca ggttaaagct
    21001 ctacgagatg gcccccttta tagaatacgc catctatgag gtaagggacg ccataattaa
    21061 attaaaggga tggcaagatg ataaaagcgg ccttaaaaaa gctgatgaat tcatatattc
    21121 caacgggtta aagatatata caaccctgga tagggatatc caaaagacgg tggaggacac
    21181 ggtctataac tgggacggat atccggattt agaggatgac gataatcccc ttccccagcc
    21241 ccaggctgct gcggtggtca tggacaataa ggggcactta aaggctattg taggagggcg
    21301 cacacctcct accaacaaga gagagctcaa cagggctata tcgcctgtta tggtgggctc
    21361 caccataaag cccgttgcgg tttacggacc tgccttggag gagggaaagt cgcctgcaac
    21421 catatatcac aatattcctg ttaagatcca aggctggaac gatcagcagg attttcccaa
    21481 aaactatggc ggcggaagtt acagcgggcc aaccaccatg agagacggtc taataaaatc
    21541 actcaatata gtagctgccc aggcccttac ctacgatgta gggtttgaaa gatccaaggt
    21601 atatctggag ggtctgggtg ttgatccgtc ccatatccag gcaaacggat cgggattggc
    21661 cctggggaca tcgtccatta cccccgttga actggcaggg gcatatagcg ccatagcaag
    21721 cggcgggatt tttcatgaac cgatatcggt tttaaaggtg gtagaccggg acggtaagac
    21781 cataatcgac agaacaggga acaggctgca gagaaaggtg tttagcgaat ccacatcatg
    21841 gttattggtg gatatgatga aggatgcagt gaagtacggg acgggtaaaa acgccttaat
    21901 acccgaaatg acagtcgcgg gtaagacggg caccaactcg gactataagg gtgtagcctt
    21961 tgcgggcttt actccctact acacgggcgt cgtttggatt ggacacgatg aaggtaagcc
    22021 cttaggcagt aatgtccagg gcggcaaata tgcagcacct ctatggcagg cctttatggc
    22081 caaaattcat gaggatgcgg gctttgagga caggaatata ttgacaatgg atcctgaagg
    22141 attgggtttg gtgcagaaaa gggtatgcaa gatatccggc aaactggcaa gtccggattg
    22201 cccccaggat gatgtaatag tggattggtt tgatcctaag tcggtaccgg aggaggtatg
    22261 cgaccggcac ggtacctttg aggtttgtgc agaatccggc aagctgccgg gcccatattg
    22321 cccggaggaa tccatatcca caagaacggc gtacttttta gaatctactt ctccgtattg
    22381 ggaactgtcc cgggatcaga taaggaagtg gatacccaag gcccatttcg aatttagcag
    22441 cttatgggag ataagcggtt tggactacag caatccggag catacaaaat acttctgcga
    22501 tattcatacg gagccgccga taccggaagc aggaggggga ttagacgatg cttttgataa
    22561 gcttaaggac ttttttgatg ggcttttccc cgggaaaaat aaaggggaga accagactca
    22621 ggaggatccc gacgacggca tcaacgaaga gaatgtgaac ggtgaagacg aacttcacgg
    22681 cgacgatgtt gacgacgggc atgaggataa cgacggttga ttgaggccgg gaatactata
    22741 attgcccttg ggagttgctc catttaccct caagggcaat tcgttatgca atttatcaac
    22801 attagacttg caaaaatctt gacatacatc cctggggggc atataattat gattaaatta
    22861 aaattctatt aactcaaaat ttcataatta tagtcatagg ggtaatgttt attggagtct
    22921 tggcgaataa tataggttat ggaattttta gattcggagg aaataatatg ggcaatggat
    22981 acaatcccta tagggagatg ctggaggttt tggatcgggc cgctgaaaag ttggggctcg
    23041 aggagcggga ctatattcaa ctaaaatatc ctgaaagaga attgaaggtc tctattcctg
    23101 tggaaatgga tgacggttcc atagaggtgt ttgaaggtta tagggtacaa cattccgggg
    23161 ttagaggtcc ctataagggg ggcatacgct atcaccagaa cgtcgatatg gatgaggtaa
    23221 aggccttggc tgcctggatg agctttaagt gtgccgtggc ggatattcca tatgggggcg
    23281 gaaaaggtgg ggtcacggtc gatgccacca aactctcaaa gagggaactt gagaggctca
    23341 ccagaaaata tgctacgctg ctctatcctg tggtaggacc tgaaatcgat attcctgccc
    23401 ccgacgtgaa caccaacgcg gaggtaatgg ggtggtttat ggatacatac agtactctaa
    23461 ggggccacct gtcacccggg gtggtaacgg gtaagcccgt tcctatcggc ggatcgctgg
    23521 gcagaggaga ggcaacgggg cgtggagtta tgcttatggt cagggagatc tctaaaaagc
    23581 tgcacctgcc tttagaaggg gccagggttg cagtacaggg tgcgggcaac gtaggtggaa
    23641 ccgctgccag acttcttcac agggaaggtt gtaaggtggt tgcattaagc gacgtatcag
    23701 gcggaatact ttcagaggac ggccttgata tggatgagat aatggatttt ctgaccgctc
    23761 agcgcggccg attgctcaag gattatgatg cgccaaattt ggttagaatc tcaaacgagg
    23821 atcttttgct tcaggatgtg gacatcctaa ttcccgcagc cctagaaaac cagattacag
    23881 aggagattgc gccccatata aaggcaagga tcgtagttga gggggccaac gggccgacta
    23941 cagtaggggg ggacaggata ctcgaagaaa aaggcatagt ggttgtaccg gatattctcg
    24001 ccaattccgg aggggtgacc gtatcctact ttgaatgggt acagaacctc caatccttca
    24061 ggtgggagga ggaggagatc agagagagat tgaaaaacat catgatcaag gcttttaatg
    24121 aggtctggga tacggccgta gaggcggaaa caaccctgag aatgggtgct tatatgctgg
    24181 cggtggatag gatagttact gccagcaaga tgaggggatt atcctaggac cgcttaatta
    24241 aataacgtaa gcaaaaaata tgaaagtggg acaccattgt ttggcgtccc actttcatat
    24301 gactaaaaca tcggaagcaa ttgcattggc aattgcaaca cacaggtttc aacgagacga
    24361 gtgatttgta cccaccgcat gtttacttta ctaatgggaa ctcaccgctt atagaaacgg
    24421 gtgacatctt gccgactcgt tatgattgca tatcgggcta atggagtggg taaacccgtt
    24481 tattcccttg tccgggcatg gacaacgaac cgggcgtcct tttcctcgta ggtacaggtg
    24541 gccagggtta gcatcccgtt attgtccgcg aagtcaatgt cagatgggtg gagggatctc
    24601 ttttttgctc cggaaaaata tttttcgtac ccttttttat cgccaaaggc aaggatgagg
    24661 tccacattat cagctctgga tatgtacgct gagaatattt catattccac ggtttttccg
    24721 cccatatcca aaaatatgct gccgttgtct tcataaaaac ttctcttttt gtacttcatg
    24781 agatccttaa acatggttcc gtccctcata tgatggccgt aaataaccaa attttcatca
    24841 actgggggat cgtttctata atccatgaag atggcgccgt gtctgtcgat atttcctaaa
    24901 aggtcatggt tcagatagta gctattatcc cttcctttga ctatgggata atcgatctta
    24961 gtacccggaa tggttatcca gcctattata tccgggttta tatcctttaa tctgttaatt
    25021 cttgaccttc cgcccgcatc tttttcatga tcctttacat cgttggtatc agccgaattt
    25081 ttatcttccc catcttctcc gctgtcggct aaaccctcat agtatatccc cgagatgtcc
    25141 ctatataggt ttcccgtatc cctagcgctt atgaggtact caccgatttg gtacatagaa
    25201 aagagcaata cggctataga gacgattaga ataaaccttc tcatggaaaa acctccaacc
    25261 agaatgaaga gtgatatctc ggtacgatgc ggtcttagct aaggccatta taccaatacg
    25321 cctgagggtt taccacacca tttttccctt aaaacgggct tttctatctt accggtgggg
    25381 tttctgggta ccttatcgaa gattaccttg cggggcctct tgtatctggg cagggaccta
    25441 caaaaatcca aaatggctgc ctcccccaac cggcgtccgg atttgggctc tatgatggcg
    25501 acgggtattt ctccaagcct ttcatccgcc atgcctatga cagcggcatc ctttatatca
    25561 tggtgttctt ggagaaaatc ttcaatctcc acgggaaaga tgttctctcc cccggaaata
    25621 atgatatcct tcttcctgtc caccagatag ataaaacctt cggaatcctg gcgggccata
    25681 tctcccgtat gaagccaacc gtcccttaag accttgtcag tctccgcctc gttgttaaaa
    25741 tagcccttca tgacccccgg gcctttgaca aggagttcgc ctgtttgacc gtaggacaag
    25801 gcattgccgc tcttatccac gattttggct tgccaattaa agcccggtct tcctatggcc
    25861 cctaccttat ggatgttttc aatgcccagg tggacacagc cgggccctgt cgattcgcta
    25921 agaccataat tggtatcata ggattgattg gggaagtgtc ttctccaccg cttgatgagg
    25981 cttgggggta ccggctgggc ccctatatgc ataagccgcc attgatccaa tttaaagtcg
    26041 tctaggtcca cttggttgga ttcgatagcc tccaggatat cctgcgccca aggtaccagc
    26101 aaccacacta tggtggcctg ttcctcgctg atggcatcca atatatactc gggttttaca
    26161 ccttttaaaa taactgcctt ggagcccacc attagactgc cgaaccaatg catcttcgca
    26221 cccgtatggt agaggggcgg aatgcagagg aagttgtcct catgggtctg gagatggtga
    26281 tggttctccg ttatacaggc tgaaaccaga ttggaatggg tgagcactat gggctttggt
    26341 gtaccggtgg tcccggaagt aaaatagagg gccgcttcct gatcgtcaga aatatcaata
    26401 ccgggagcgt ctgtgggaac atccctaata atatcaaaaa aattggctat gccatccata
    26461 ccggatggct gtccatcgcc gacataaaaa gcgtgttcca ggcacttggc tttgccggct
    26521 atgcctctaa tccgatcctc gaattccggg ccgaagatga gtatctttgc cccggcggtt
    26581 tccaggcatt tttttatctc ggggcccgta aaccgaaaat ttaggggtat tgccaatgcg
    26641 ccggttttta gaataccaaa atagatgggg agccattcca gacaattcat cattaggatg
    26701 gctaccttgt cacttttttc tatgccatgg gatattaaag cgttggctaa tcggttggac
    26761 atgttgtcaa attctgacca ggtaatttcc cggcggagcc ccagggccgg atccctttca
    26821 ataaggctgg ttttattggg atatatctgt gcattcatgg atagcatttc cgtgataggc
    26881 atcaaaagaa acctccgttc taccgttcta ctattgtgga tgaattaagc aatctactgt
    26941 actgtctgta ctaccctatt gttttgatgt ataattgcac ttgtccggaa gatgggactt
    27001 agtgaaaatt atatcaacga tatatagata tattgtatgg agaatccggg cacctgtcaa
    27061 gggcgtattc tcaataaggg tgcataatat aaatttgaag aataatattc caatcactta
    27121 agttccaagg ctaagaacct gtaaatttgc tccggggcct tctcaaactc gctacgctca
    27181 aacagtgatt cggcctatcc tccgctttat taacaggttc taagcccctt cacaatgtga
    27241 tctccatata attcttcaaa taaaaaaaca cgccctctca acaatatatt gtatatgatg
    27301 ctaattatag ctctttttgg atatttatcg tttaacaatc ctaatgggaa agggcatcaa
    27361 tttcaaatgg ggtaaactat tcgattagga tcagcatggt cgttataggg agaatgcatt
    27421 tttgcagaat gtgtgtaaaa gggaatgctc tatcgtataa aggacaatgg aacgtcagtt
    27481 ggaggcacat gaaattgata ggaggtaatg attttggaaa gacagtttca tattgattta
    27541 gatgatggtc acggtgccga gtatgctata ctgccgggcg atcccgaaag ggtggaggag
    27601 atagcaggat accttaaaaa ccccgaattt ctcgcctcta aaagggaata caccagctgg
    27661 atcggcgagc tgggcggcga aaaggtcata gtaacctcca ccggtattgg gggcccctcc
    27721 gcttcaatcg ccatcgagga gctatacaga acaggggtca gaaccttcat aagggtggga
    27781 acctgcgggg gaatgcaact tgatgtagtg ggcggcgatg ttgtcattgc gacgggctct
    27841 atcagaaacg agggaaccac cagggagtat atgcccattg agtttcccgc agtcgcggac
    27901 tttcatgtgg tacaggcgtt ggtacaggct gcagaaaggc tgggatactc ctatcacacc
    27961 ggggtggtac aatccaagga ttccttctac gggcagcata gccccgagag gatgccggcc
    28021 gggtatgaat tgttaaacaa gtgggatgca tggattaggg ccggggccct agcgtcggag
    28081 atggaaagtg cggctctgtt ttgtgtatcc tcctatctta gggcccgagc gggctgcgtc
    28141 ttaaacgtta tatggaatca ggagcggggg aaggcgggca tggataatcc ccggcatcac
    28201 gataatacca gggcaataat caccgccgtc gaggccataa agatcctgat cgaaaggtag
    28261 gggccggctc tcttgcttag ggagaaaaac ggaagcacgg gggagaaaca aaggggttat
    28321 ttttttcccc aaaataaaag tgaagcaaag gggattatcc ctttgcttca cttttattta
    28381 cgttctctgt gagactatta tactataggg ctttcagaca tagccttgta gatattgtat
    28441 ctgtccttgg catcgttttc ggctacctgg aagaattcct ttgcaacctc cgggaaggtc
    28501 ttggccaagg tggtatagcg cacctcggta ttcaagaaat cctggaagga ttctgtgggc
    28561 tccttagaat ctaagatgaa ggggtttttg ccttcttcct ttaggagcgg gttgtagcga
    28621 tataggtgcc agtagccgga ttgtacagcc agcttcgttc tgctggagct gttaaccatg
    28681 ccgcccctca ttccatgtgc gatacacggt gagtaggcaa tgatcaacga tggtccggga
    28741 taggcttcag cctctctcag aacccttacg gtgtggttca tatcagcgcc gagaccgatc
    28801 tgggcgacgt atacatagcc gtaagtcatg gcaatgcggc ccaaatcctt cttgcgaata
    28861 cgttttccgg ctgcggcaaa cttggccaca gcggctgtgg gcgtagcctt tgaggcctgg
    28921 cccccggtgt tagaatagac ctcggtatca aagacgaata cgtttacatc atcgccggat
    28981 gccaatacat ggtccaatcc gccaaagccg atatcgtagg cccaaccgtc accgccgaaa
    29041 atccactggg attttttaat caggtattgt tttagatcaa gaataccctt aacaacggcg
    29101 ttgtcattgt gtttattcaa taagggaagc aattccttcg tagctgcttg ggattcttcc
    29161 ccaagatcct tgttctccag ccatttgttt aaagcctgtg taagatccgg atcgatgttt
    29221 gcatcgatgg cttcatcaac cagaccggcg agtctatcgc ggttttgctg taccccaagg
    29281 tgcatgccga aaccatattc cgcattatct tcaaacagag agttggccca tgcgggtcca
    29341 cagccctctt tatttaccgt atatgggcta gcgggatagg aaccgcccca gatggagctg
    29401 catcccgtag cattggctat gatcattcta tccccaaaga gctgagtcaa cagcttggca
    29461 tatggagtct caccacagcc ggcacatgcc cccgagaact cgaataaggg ttcttggaat
    29521 tggctaccct ttaaggtgaa tttattcata aggtccttct tgggggaaac ctcattgatg
    29581 gcatactccc agaggggttt ttgtttttct atctgggtat ctaggggttt cataacaaga
    29641 gcttttttcc tggatgggca aacgtcagcg cagaccccgc atccggtaca atccaaggtg
    29701 cttacctgga tcctgtatcc cattccctta gctgttctgc cgatggcagg cttggtctca
    29761 aaatcctcag gtgcgttggc catttcatct tcgtccaata cgaagggtct gatggctgca
    29821 tggggacata ccagggaaca ttggttacac tggatacaat tggcaatctg ccattgggga
    29881 acctgggctg caatacctct cttctcataa gccgcagttc cggcgggcat ggtgccgtct
    29941 tcaagtccta caaaggtgct gacgggaaga tcgtcgcctt cttgccttgc tatgggctct
    30001 aggatattct taataaaagc gggcttgtta tcgtcatcag agacaacctt gccatccttc
    30061 acatcggccc agcttgcggg tacctccacc ttaactaaag cggccaaggc ttggtccaca
    30121 ccatcaaagt tcatcttgac tatatgttcg cccctatggc cgtagctatt ttggatagcc
    30181 tctttgaggt aacctatggc ttcgtccacg ggaatgatgt tagccagttt aaagaatgcg
    30241 gtctgcatta tcatgttgat tctgccgccc aggccaacgc cctcagcgat atgaagggca
    30301 tcgattatat aaaaatctat attgttcttt gccaaatatc gcttaatgga agccggcaac
    30361 ttctcgtcca actcctcggg gctccaggtg cagttgagta ggaaagtgcc gttttcggca
    30421 agtcccttaa ttacatccaa ttggtctaca tatgcctcgt tatggcaggc gatgaaatcg
    30481 gcttcatcta ttaaataggt ggattttatg ggttgctccc caaagcggag atgggagacc
    30541 gtaactccac cggatttttt agaatcgtag gagaaatacg cctgggcata tagatcagta
    30601 ttgtcaccga taatcttgat agcattttga ttggcaccaa cggtaccgtc agagcccaga
    30661 ccccagaatt tgcagcggac cgtcccctta ggtgaggtgt tcacatactc agaggggatt
    30721 aaggatgtgt tggtcacatc gtctatgatc cctaaagtaa agccgtcctt ggcatctgcc
    30781 tctttgaggt cgtcaaacac agccttgata tgggtaggtg tggtgtcctt tgaacccaag
    30841 ccatatctgc cgcctgtaat ggtcggggca ttttcccttc catataggat agctttgata
    30901 tcctgataca ggggttctcc cacagcgccc ggctctttgg tgcgatccag gactgcaatc
    30961 ttcttaacgg tggacggtaa aacgtccagg aagtattttt cagagaaagg cctgtataga
    31021 tgaaccttaa tgagccctac tttttctccc atggccaata agtaatccac ggtttcttct
    31081 attgtctgtg tgacggagcc catagccact ataatgtgtt cggcatctgg tgcgccgtag
    31141 tagttaaagg gcttatattc acgaccggtc agttctgaga tcttgatcat gtactcattt
    31201 acgatatcag gtaccgcgtt gtaaaaggga tttgcggcct ctcttgcctg gaagaaaacg
    31261 tcagggtttt gggccgtgcc ccttgtgacg ggactttccg ggctaaggga atttttgcgg
    31321 aatctatcta tggcttcata gtccaccaat gaagctaagt cgtcatattc tagtacttcg
    31381 attttttgat actcatggga agtccggaaa ccgtcaaaga aatgtagaaa cgggacccta
    31441 gatttaatag cagccaggtg cgctattccg gccaggtcca tgacctcttg gacactcccg
    31501 gatgccaact gcgcaaatcc ggtttggcga gtagccataa cgtccgaatg atcaccaaaa
    31561 attgaaagcg catgggctgc tatggcgcgg gcgcttacat gcataacccc cggcaatagt
    31621 tcccctgcca tcttgtacat atttggaatc ataaggagca acccttgaga tgccgtaaag
    31681 gtggttgcca acgatcctgt tgccaatgca ccgtgcatag cgccggcagc gcctgcctca
    31741 gattgcaatt cggacaacct tacggtttgc ccaaatatat tttttcgacc atgtgcactc
    31801 cattcatcga ctacttcagc catattagac gatggagtaa tggggtagat agcagctaca
    31861 tctgtaaacg catatgcgac atgcgaggca gcagtattgc catccatagt cctcatcttt
    31921 ttacccatat gaatgaccct cctataataa gttaattagg aagcttcaaa aaagactttg
    31981 ttaaatgaga cgggatataa tgttgataat ccgagttttt tgaaacttca actgatcaga
    32041 tatttttagg taaaacattg tacatatttt acataacaat tctattacgt atatagggat
    32101 ttgtaaaggg tatattgata aaacaacgga ttccgtcact tttttgtcaa tgcccctgga
    32161 atattctact taaaaggcta tattttggtc taaaaaccca tgcttttcag ggatccatcc
    32221 acagcgaaca tcaccttcat cgtttacgct acatccatat gt
//
LOCUS       DASRNX010000138         4279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_28213,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000138 DASRNX010000000
VERSION     DASRNX010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28213"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFD89_05715"
     CDS             <1..814
                     /locus_tag="VFD89_05715"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZJ57723.1"
                     /translation="FLGDFRPDIVICTHVFAAQIINYLQRKGTIDTKSIGIITDFTIH
                     PFWEGTDLDFYVTANELLTWQASKKGIDTKKILPIGIPIHPKFGLKMDPSEARKCLGV
                     EDKTTVFIMGGSMGYGDLESILMDLDDLDMDFQIISVCGSNKNLKKRIDGMIIRKRIH
                     NFGFVDNIDIIMDAADCIITKPGGLTTSESLAKGIPMILINPIPGQEERNMEFLINNG
                     LAIMVTETYPAGEALYQLMNNPWRRKFMIRMTEMFGKPNATRDLCDHILIVS"
     gene            1094..2422
                     /locus_tag="VFD89_05720"
     CDS             1094..2422
                     /locus_tag="VFD89_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HZJ57724.1"
                     /translation="MFIPKSKKYVKACAAVGLSAMMFFSTPSLSSAAVQLRLGSSGNE
                     VQKVQSQLKDLGFFKYPSITGYYGTITRDAVIKFQAAKGISQDGVVGTTTRKYLFTTG
                     TSESGGAANASNPVLRKGSRGAAVTRLQELLKAKGYYKNGSIDGSFGGITYSAVIAFQ
                     KGSGLQVDGVAGPMTWAALLSEGSSPKGSGSSGITSTGALKRGSKGGAVTDLQKVLKD
                     LGYLSGKVDGSFGPQTERDVKAFQKANGLVVDGKAGPKTMARLNDIIAKGQGSTSDRG
                     SEGKPTPPISGAVNELLAWADVDKLWPRNSYATITDFDTGKTFKVMRSGGYNHADVET
                     ATAADTEIYKEIYGGVYSWNRRAIVVSVNGRTIAASMAGMPHAGRDDKPNRAMVYDRS
                     CDYGYGQNLDAVKGNNMDGVFDVHFYKSKTHATNKVDDKHQAQVKRAAGK"
     gene            2628..2855
                     /locus_tag="VFD89_05725"
     CDS             2628..2855
                     /locus_tag="VFD89_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HZJ57725.1"
                     /translation="MNEEFRPILGLGSVGPEVEMLQRILFSIGINPGPIDGIFGPLTR
                     NAVVRFQMDNGLVPDGIVGPETWAAIDLVYP"
     gene            complement(2940..4043)
                     /gene="aroC"
                     /locus_tag="VFD89_05730"
     CDS             complement(2940..4043)
                     /gene="aroC"
                     /locus_tag="VFD89_05730"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZJ57726.1"
                     /translation="MSGSIFGKNFSIATWGESHGKALGVVIDGCPAGVSLDVHDIQKD
                     LNRRKPGRSPYATPRSEDDSVEILSGIFEGRTTGTPISMVIFNRDQRSRDYGNIKNTY
                     RPGHADYVYDQKYGFRDYRGGGRSSGRETAARVAAGAVAKKILSLLGIDILTYTLSIG
                     NIITNPNRFDAEEILLNPLAMPDREASVRAGLFLDQLKEKGDSAGGVIECMVNGLPPG
                     LGEPVFEKLDSVLSKAIFSIGAVKGLEFGIGFGAATLTGSQNNDPLMMDPQGKITKET
                     NNAGGITGGISDGSPIVFRAAVKPTSSISLPQKSIDRDAGKNIEIVISGRHDPIIVPR
                     AVIVVEAMTAITLVDFLFQRILSRMDLIQKALL"
BASE COUNT         1189 a          912 c         1088 g         1090 t
ORIGIN      
        1 ctttttgggg gactttaggc ccgatatagt gatctgtacc catgtctttg cggcacagat
       61 cataaactac ctgcaaagaa agggaactat cgatactaag agcataggta tcatcacgga
      121 ttttactata cacccgttct gggagggtac ggatctcgat ttttatgtca ccgccaatga
      181 actgttgacc tggcaggcct ctaaaaaggg aatagatacg aaaaagatat tgcccatagg
      241 tatacccatc catcccaagt ttggattgaa gatggatccg tcagaggcca gaaaatgctt
      301 gggcgtggaa gataagacca cggtttttat tatgggcggc agcatgggct atggggacct
      361 ggaatcgatt cttatggatt tggacgattt ggatatggat tttcagataa tatccgtatg
      421 cggaagcaat aagaacctga agaaaaggat agatgggatg atcatacgca aaaggataca
      481 taattttggc tttgtagaca atatagacat aataatggac gccgcagatt gtatcatcac
      541 taaaccaggg gggctgacca cctcggagag tttggctaaa gggataccca tgatccttat
      601 aaatcccata ccggggcagg aagagagaaa tatggaattt cttataaata acggattagc
      661 cataatggtc acagaaacct atccggccgg tgaggctctg tatcaattga tgaacaatcc
      721 atggagaaga aaatttatga tccggatgac tgaaatgttt ggaaaaccaa acgccacccg
      781 ggatctatgc gaccacatct taatagtttc gtaataaatc cgtaaaagta ttttaaaaaa
      841 cccttgaatt tgtcatatat atattatatg attatcctat atatgttacg gaaaagacct
      901 tttactgcaa aatcggctaa attatccgat tctacgttag aacaatgtat tttcataata
      961 tacgaagtat ttttcgtagc atacgaatta aggataatgt tttatggcat ccgtgtgcca
     1021 atgtggaatg tttgtcgtaa aacatatagt caatcacaga gtatattgac ttattttgta
     1081 ggggggaatc tgcatgttta taccgaaatc taaaaagtat gtaaaggcgt gtgctgctgt
     1141 cgggctatcg gctatgatgt tcttttctac accttccctt tcatccgcgg ctgtccagct
     1201 gagattgggc agttcaggca atgaggtcca gaaggtgcaa tcccagttaa aggatttggg
     1261 gttttttaaa tatcccagca tcacgggata ctatggtacc atcacccggg atgcggtgat
     1321 aaagttccag gccgcgaagg gaatttccca ggacggggtt gtaggtacaa ccaccagaaa
     1381 gtatctgttt actacaggga cttcggaatc cggaggagcc gctaatgcat caaatccggt
     1441 tctcagaaag ggcagcaggg gagcggcggt aacccggctt caggagctgc ttaaggccaa
     1501 gggatattat aaaaatggca gcatagacgg aagctttggt ggtattacat actctgcagt
     1561 tatagcattt caaaagggaa gcggtctaca ggtggacggg gtagcgggcc ccatgacttg
     1621 ggctgccctc ctatcagagg ggagttcgcc taagggatcg ggatccagtg ggattacaag
     1681 cacaggggcg ttaaagaggg gcagcaaggg cggggcagtc accgaccttc aaaaggtcct
     1741 caaggacctg ggctatctgt ccggcaaagt cgacggaagc ttcggccccc aaaccgaaag
     1801 ggatgttaaa gcgttccaaa aagccaacgg tctggtggtg gacggaaaag ccggtcctaa
     1861 gaccatggca agattaaacg atatcatcgc caaggggcag ggttccacaa gcgaccgggg
     1921 cagtgagggt aaacctacac cgccgatctc cggagccgta aacgagctgt tggcatgggc
     1981 ggatgtggac aagctatggc ccaggaattc atatgcgact ataacggact ttgataccgg
     2041 gaaaaccttt aaggttatgc gctccggcgg atataaccac gccgatgtag agacggcgac
     2101 agcggcggat acggagatat acaaggagat atacggggga gtatactctt ggaacagacg
     2161 agccatagtg gtaagtgtaa atggaaggac tatagccgcc agcatggccg gtatgcctca
     2221 tgcgggtcgg gacgacaaac ccaatagggc aatggtctat gaccgaagct gcgattatgg
     2281 gtatggtcaa aacctggatg ctgtgaaggg taataatatg gacggggtat tcgacgtaca
     2341 cttttataag agtaaaaccc atgccaccaa caaggtggac gataagcacc aggcccaggt
     2401 aaagagggca gcaggaaaat aatcgacata gatgggtatc acccaaattt cccgattcat
     2461 ataataaaag taaggcaatg cctcgtgctg gttcattatc tcaattgtat ggtgaaagca
     2521 atgggctata gccattaccg atatatttaa ccagtataac cggttaaggt atatactttt
     2581 tatggtaaag gcatagcatg tacttttatg atgggtgggt gatatgaatg aacgaagaat
     2641 ttaggcctat cctgggtttg ggatccgtag gtccggaggt ggaaatgctt cagaggattc
     2701 tctttagcat cggtataaat cccggcccca tagacgggat cttcgggccc cttaccagga
     2761 atgcagtggt aaggtttcaa atggataacg gattggtacc cgacggtata gtcggccccg
     2821 aaacttgggc ggctattgat ttggtgtacc cataggagtt taaaaagcgg ggctgtccga
     2881 acgggctgca atggtagccc cgcttttttc atatccttta agtggccaag ccccatgtgt
     2941 tacaaaaggg ctttttgtat taaatccatg cgggacaata tacgctgaaa cagaaaatcc
     3001 accagggtta ttgcggtcat agcctctact acgataaccg ccctggggac aattatggga
     3061 tcgtggcggc cgcttatgac gatctctata ttcttgcccg cgtccctgtc aatgcttttt
     3121 tggggcaggg atatagacga ggtgggcttg acggctgccc tgaatacaat gggagaaccg
     3181 tcgcttatac cccccgttat gccgccggcg ttattcgttt ccttggttat cttgccttga
     3241 ggatccatca tcaaggggtc attgttttgg gacccggtca gagttgcggc accaaaacct
     3301 atgccaaatt ccagtccctt gacggcaccg atggaaaata tggctttgga aaggacggaa
     3361 tccagctttt caaagaccgg ttctccaagt cccgggggca ggccatttac catgcattct
     3421 ataaccccgc cggcggagtc acccttttcc ttgagctgat ctaaaaatag cccggccctt
     3481 actgatgcct ccctgtcggg catggccagg gggtttaaca gaatttcctc ggcatcaaat
     3541 ctgttaggat tcgtgattat attgcctatg gaaagggtat aggtaagtat atcgatgcca
     3601 aggagggata ggatcttttt agcaactgcc cctgcagcaa cccgtgcggc ggtctctctg
     3661 cccgaggagc gacctccccc cctataatct cggaatccgt atttttgatc atatacataa
     3721 tcggcatgac cgggcctata ggtattcttt atattgccgt aatccctaga cctttgatcc
     3781 ctattaaata tcaccatgga tataggggtg cccgtagtcc tgccctcaaa gattccggat
     3841 aatatttcta cgctgtcgtc ctctgagcgg ggggtggcgt atggagaacg tcccggcttt
     3901 cgtctgttta gatccttttg aatgtcgtgg acatctaagg aaaccccggc agggcaaccg
     3961 tctattacta cccccagcgc ctttccatgg gactcgcccc atgtggctat gctgaagttt
     4021 ttaccgaaga ttgaacctga caatgctatc accgcctaaa tgagaatatt aaacaatata
     4081 ttagctgtcc ctattggatt gattaatgcc attgaaccat atataaaccc gaatttcaat
     4141 tttatcacaa aaactgatgt cttggtataa attctccggg atttcggaca aggtattaaa
     4201 aaacaaccca cgggttggga ccatgaaatg cttaaaccgg tgtattcatt aatattcttt
     4261 atataggtgg atataaatg
//
LOCUS       DASRNX010000139         6768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_241953,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000139 DASRNX010000000
VERSION     DASRNX010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241953"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VFD89_05735"
     CDS             <1..774
                     /locus_tag="VFD89_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HZJ57727.1"
                     /translation="IMKGQTQEQGGQAGGMSIQVDSPIIHPKLILGCHVVPDRWLLQG
                     GSVGGGGTLKWFNQQLGAFEQSLAEERGISPYEIMSEEAAGVDPGSGGLIFLPYMAGE
                     RSPIWDSYARGVFFGLSYDKTRGHMIRSIMEGVGFSLLHNLETAKEVGAYADILNSVG
                     GAANSHVWTQIKADITGKTINVPYSDHATSLGAAILAGVGTGVYRDFEHGVDETVRIQ
                     RTHYPDERNHRIYKGYYELYLELYERLRDSFVKLYDLHT"
     gene            1119..2282
                     /locus_tag="VFD89_05740"
     CDS             1119..2282
                     /locus_tag="VFD89_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ57728.1"
                     /translation="MKDTLNIGLVGYKFMGKAHSNALQKVGMFFDPSLKIRMRAICGR
                     DEEWVKESAEKFGWEGYETSWENLIKRDDIDAIDITAPSNFHKEIAIAAAKAGKHIFC
                     EKPLALNLKDAREMLDVVTKANVKHQIGFNYRFAPAVQLAKKIIDEGKIGKVYHYRAL
                     YLQDFIIDPSFPLVWRLDKDVAGSGSHGDLGAHFVDLARFLVGEFDEVIGMSETFIKE
                     RPIVERMTGLSGKAQEGAELGKVTVDDATIFMARFKNGALGSFEATRFAAGHKNDMFF
                     EINGSKGSIRFNIERMNELEYYSASDEEGLQGFRLIQVTEDIHPYADAWWPVGHVIGY
                     EHTFVHELYEFIEAIAKDKPTNPSFYDGVKCSQILEAVDLSIESRRWVEVDSL"
     gene            complement(2616..3002)
                     /locus_tag="VFD89_05745"
     CDS             complement(2616..3002)
                     /locus_tag="VFD89_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57729.1"
                     /translation="MTGKPFPVITDIKAIPLWRGQGLVVRNIKVAVAVNLTGFPSAVR
                     QRVSVSKASLPVLIMLLPLAFISPKVIYLRIWDLTLAMSSMGLNRRLQPIHTGLPYIR
                     TPFPKNYTIVASLNRLALHRFDEYAR"
     gene            3072..4604
                     /locus_tag="VFD89_05750"
     CDS             3072..4604
                     /locus_tag="VFD89_05750"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZJ57730.1"
                     /translation="MDEFWNSKGIDQDAPDIRGCQISLAVRDELVNAPHEYSRLYSIK
                     KNLTKEKYLYLGLLAAFIAGFGLLIFSVANQNYRQELGKVIGRVLGKVFLEFKILFLL
                     TVWIILNDILRGYHWAGILRILSAATIALWLAYFVFIDIHTNHGDILTKNMIWKMVKW
                     YRQLEGNRPFHKGQLLRLRTLIVLEAIFIFFTFVLVMMAATGSEGIFLVSVFVLMGVG
                     IFLFYLYTRRFAHNLMDIDMVVEHTGRIRKGDIDTRLQLNPGSDLKVLEENLNTIQEG
                     MANAIENSIKSERMKVELITNISHDLKTPLTSIISYVDLLSKEGGLSDEVRDYVRILG
                     QKSDRLRLLIQDLFDLSKVVSGQMEFHMKTLDLGKLIQQTLADLDDKITQSGLEFRVN
                     LPDDPVLITSDGNKLYRVFANLFSNALKYSLIGTRVYVDLTVEDGSAVAVIKNTANYE
                     MNFSEEEITERFVRGDKARSSEGSGLGLAIAQNFTRSCGGDFHISVDGDLFKVILSFD
                     IL"
     gene            4905..6188
                     /locus_tag="VFD89_05755"
     CDS             4905..6188
                     /locus_tag="VFD89_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZJ57731.1"
                     /translation="MNYTQNEKIMSITERTLVVGVDIAKSIHYARAFDYRGVELAKVF
                     RFENTREGLELLKEWIGTLERENNKSTSIIGMEPTGHYWFNLGHYLKDEGIRLVLVNP
                     FHVNRSKELDDNSPTKNDQKDPKTIAKLVIDGRYSIPYIPEGIYRDLRIVMDMRDSLS
                     KSLIRIKNKVTQWLDKYFPEFFNAFADWEGKAALITLREFPTPDKIVAAGIDDIVVTW
                     RKEVKRGVGAKRAARLVEAAKGSIGIREGLRMAQKELRAIMQQYDLYMEQLGEVETDM
                     EELALQVPGVSEMLTIKGVGILTAAAFMAEVGDISRFSHPSQIQKLAGLNLVESSSGK
                     HKGQTTISKRGRSRLRSTMFKVMMPLVAKNDEFKALHEHYTTRRENPLKKKQSLILLS
                     CKLIRIFYALITKEVAYSPEKLLKNISYLQLQDAA"
BASE COUNT         2053 a         1259 c         1673 g         1783 t
ORIGIN      
        1 attatgaagg ggcaaaccca ggagcagggg gggcaggccg ggggcatgag tatacaggta
       61 gatagcccca ttatccatcc taagctgatc ctgggctgtc acgttgtacc cgatagatgg
      121 ctcctacaag gcggcagtgt agggggcggt gggactctaa agtggtttaa ccaacagctt
      181 ggggcctttg aacagtcatt ggctgaggaa cggggaatca gtccctacga gattatgagt
      241 gaagaggcag cgggggttga cccgggaagt ggcggcctta tatttttgcc ctatatggcc
      301 ggggagaggt ccccaatttg ggacagctat gcaaggggtg ttttctttgg gctatcctac
      361 gataagacta ggggacatat gatccgctct ataatggagg gtgtaggctt ttcacttttg
      421 cataaccttg aaacggccaa agaagtagga gcctatgcag atattctgaa cagcgtcggg
      481 ggagccgcca acagccatgt atggacccag ataaaggctg atatcactgg gaaaactatt
      541 aatgttccct attcagatca tgctacttcc ctgggtgcag ctatccttgc aggggtgggg
      601 acaggggtgt atagggactt cgaacatggt gtagatgaaa ccgtgcgaat tcagcgaacc
      661 cattaccccg acgaaaggaa ccataggatt tataagggct actatgaatt atatctggaa
      721 ctgtatgaaa ggcttaggga ttcgtttgtt aaactatatg atctgcatac ctaaccccta
      781 tgccacgaaa tgacgggaac ttttggcatt ttctctgtgt ttttgccgat atcagcttga
      841 aaacgggccc gattatagta taaactttta ttaatgaagc acgttgcgtt attttatcca
      901 tttaccatgt aacaaaggca aggggctgca taaaaatgga tgtcgggaag tttcaaaaat
      961 acgttattaa tagcatctgc tacaataatg ttaatttgct tttttgaagc ttcaactgcg
     1021 cacttaccct tatggcgggg cttgacgttc aatagtcatt gccgaaatat taaattggag
     1081 ctgcaggtta aaaaataaaa tttataggtg gtgttagcat gaaggataca ttgaatatcg
     1141 gtttggtggg ttacaagttt atgggcaagg cccacagcaa cgctctccaa aaggtcggaa
     1201 tgttttttga tccgagtcta aagatcagaa tgagggccat ctgcggaagg gacgaggaat
     1261 gggttaagga atccgctgaa aagtttggct gggagggtta cgagacctct tgggagaatc
     1321 tgatcaaaag ggatgacatc gatgctatag atataaccgc ccccagcaac tttcataagg
     1381 agatagccat agcggctgcc aaggccggga agcacatttt ctgtgagaag ccattggctc
     1441 taaaccttaa ggatgcaagg gagatgctgg atgtggttac aaaagccaat gttaagcacc
     1501 agataggctt taactaccgc tttgcaccgg ctgttcaact ggccaaaaag atcatcgatg
     1561 agggcaagat aggcaaggtc tatcactata gggctctgta tctgcaggac tttattatag
     1621 acccgagctt ccccctggtg tggaggctgg ataaggatgt ggcaggttcc ggctcccatg
     1681 gggatttagg tgcccatttt gtggatttag ccaggttctt ggtaggtgaa ttcgatgaag
     1741 taataggtat gagcgagacc tttataaagg agcggcccat agttgagagg atgaccggcc
     1801 tcagcggcaa agcccaggaa ggggcggagc tggggaaggt aacggtagac gatgccacca
     1861 tatttatggc gaggtttaaa aacggcgccc tcggttcctt tgaggctacc cgttttgctg
     1921 ccggccataa gaacgatatg ttctttgaga taaacggcag taaaggcagt ataaggttta
     1981 atatcgagcg gatgaatgaa ctggaatact attctgcttc agatgaagag ggtctccagg
     2041 gtttcagact cattcaggtt acagaggata tccatcccta cgcagatgcc tggtggcctg
     2101 tgggacatgt tatcggttat gagcacacct ttgtacatga gctgtacgaa tttatagagg
     2161 ctatagccaa ggacaaaccc accaacccca gcttttatga tggggtaaaa tgctcccaaa
     2221 tccttgaagc ggtagacctc tccattgaaa gcaggcgatg ggtagaggtg gacagcctgt
     2281 aggattatta acctacatac cggggcaatc cattcaaaga ctaaaaatgc tgtatgactg
     2341 gcgcaatggg cggataccac ctgttgcgcc ggacttttat cgggaaaagc ctgtagtgta
     2401 tgaattggcc tatcgtgcta gttgaacata ccggtaatgg ctattgaacg cttatcgcgc
     2461 aatgagttga tgagcttgcg caacgcggta attaagattt ccgtaagaaa taggataact
     2521 tttttgtaag gatattcgtc tatcataacc atagggcgaa gtggtttatt ctatgatgaa
     2581 ataaatgcag ccaataaagt aaaacctttg gggtttcatc tggcgtattc atcgaatcgg
     2641 tgcaaggcaa gccgattaag gcttgcaact atggtataat ttttggggaa gggggtgcgg
     2701 atatatggaa gccccgtgtg tattggttgt agacgacgat ttaatcccat ggagctcatg
     2761 gccagggtca aatcccagat acgaaggtat atgaccttgg gtgatatgaa cgccaaagga
     2821 agtaacatta ttaggaccgg aaggcttgct tttgacaccg aaacccgctg cctgaccgca
     2881 gacgggaacc ctgtcaggtt aacagcgacg gcgaccttga tatttctgac aaccaacccc
     2941 tgaccccgcc aaaggggtat ggcttttata tccgttatga cgggaaaagg tttaccggtc
     3001 aagatgcggg caatccccta gatatacaca acagcaatag tggatattgg aacacagcga
     3061 tttatagtta tttggatgaa ttttggaatt caaaggggat tgatcaggat gctcccgata
     3121 ttcggggctg tcagatatcc ctggcggtcc gggacgaatt agtaaatgcc ccccatgagt
     3181 atagcagact gtattccata aaaaagaatt tgactaagga aaagtacctg tatttaggcc
     3241 tgttggccgc ctttattgca ggctttggct tgctcatatt ctccgttgca aatcaaaact
     3301 acagacagga attggggaag gttataggaa gggtcttagg caaggtattt ctggaattta
     3361 agattctatt ccttcttacg gtatggataa tcttaaacga tattttaagg ggatatcact
     3421 gggcaggcat tttaaggatt ttatctgccg caactatcgc cctgtggtta gcatattttg
     3481 tctttataga catccacaca aaccatggag acattctcac aaagaacatg atatggaaaa
     3541 tggtaaaatg gtacagacaa cttgaaggga accgaccctt tcataagggg caactcctca
     3601 gactgcgtac cttgatcgta cttgaggcga tatttatatt ctttaccttt gtgcttgtta
     3661 tgatggccgc taccggcagc gaaggtatat tcctcgtctc ggtatttgtt ctgatgggtg
     3721 ttggtatttt cttattctat ctctatacga ggcgatttgc acataattta atggatatag
     3781 atatggttgt ggagcatacg ggtcgcataa gaaagggcga tatagatacc aggctgcagc
     3841 taaaccccgg ttcggacctg aaggtcttgg aagagaatct aaataccata caagagggga
     3901 tggccaatgc cattgaaaac agtattaaga gcgaacggat gaaggtagag cttataacca
     3961 acatatccca cgatctaaag actcccttaa cgtccattat aagctatgtg gatctcttat
     4021 caaaggaagg cggattatcg gatgaggtaa gggattatgt aaggatactg gggcaaaaat
     4081 ccgatagatt aaggctcctc atccaggatc tcttcgactt gtccaaggta gtgagcggac
     4141 agatggaatt tcatatgaaa accctggact tagggaagct tatacagcag accttagctg
     4201 atctggatga taagatcaca cagtccgggc ttgaatttag ggtaaatcta ccggatgatc
     4261 ctgtactcat aacaagcgat ggcaacaagc tctatcgggt gttcgcaaac cttttttcaa
     4321 acgctttaaa atattccctg ataggtacca gggtatatgt ggacttgacc gtcgaagacg
     4381 ggagcgccgt tgcggttata aagaacaccg caaactacga aatgaacttt agtgaagaag
     4441 agataaccga acggttcgtc aggggggata aggcacgctc cagcgaaggt tctggattag
     4501 gccttgccat tgcccaaaac tttacccggt cttgtggcgg agacttccac ataagtgtgg
     4561 acggagacct atttaaagtc atattatcct ttgatatact ctagaaataa ttattgaaaa
     4621 ttatgatgca taatggtatg cttgacttaa cattatgcaa cgtcaagcaa atgaccggtg
     4681 aattgaaatt ttgaaaagtc caattgtcgg tgttatgacc tcgagtatca ataatgtatt
     4741 ttttgaaact ttcttaatga caaatatagt aaacattata taaagcattc ctgtaatgaa
     4801 ctaatttgaa ctttgaaaaa agaagtacct cctgatacaa tacggggtat aaggtgttgc
     4861 aacacttatc ccttattaat aaatatcaga tggaggtacc tcaaatgaat tatacacaga
     4921 atgagaagat aatgtcaata acagaaagaa ctttagtagt gggagtagat atagcgaaat
     4981 ctatccatta tgccagagcc tttgattatc ggggcgttga gctggcaaag gtttttcgct
     5041 ttgagaacac tagagaagga ttagaactgt tgaaggaatg gataggcacc ttggaaagag
     5101 aaaataacaa gagcaccagt attattggaa tggagcctac agggcattat tggtttaacc
     5161 taggacatta cctaaaggat gaggggatac gattagttct tgtaaatcca tttcatgtga
     5221 atagaagtaa ggagctagat gataattctc caaccaagaa tgaccaaaag gatcccaaaa
     5281 caatagcaaa gctggtgatc gatggtagat attcaatacc atatatccca gagggtatct
     5341 atagggattt aaggatagtc atggatatga gggattccct aagcaagtca ctcatcagaa
     5401 ttaagaacaa agtaacgcaa tggcttgaca aatactttcc ggagttcttt aatgcatttg
     5461 ctgattggga aggaaaggct gccctaataa ctttaaggga gtttccaaca ccggataaga
     5521 ttgttgcagc tggcatagat gatattgtag tcacatggag aaaagaagta aaacgaggag
     5581 taggagctaa aagagcagca aggctagtcg aggcagcaaa aggatccata gggattagag
     5641 aaggacttag aatggctcag aaggagctca gggctattat gcagcaatat gatctatata
     5701 tggagcaact gggtgaagta gaaacagata tggaagaatt ggctcttcag gtaccgggag
     5761 tcagtgaaat gctaactata aaaggcgtag gtatacttac agctgcggca tttatggcgg
     5821 aggtaggaga cataagtagg ttctcacacc ctagtcaaat acaaaaatta gcaggcctaa
     5881 acctagttga aagtagctca ggaaagcata aaggacaaac taccattagc aagcgaggac
     5941 gctccagact ccgaagcacc atgtttaagg tgatgatgcc ccttgttgcc aaaaatgatg
     6001 agtttaaggc gctccatgag cattacacta cccggcggga aaacccgcta aagaaaaaac
     6061 aatcattaat tctgctaagt tgtaaattaa taagaatatt ttatgccctt atcactaagg
     6121 aagtggctta tagcccagaa aagttattaa agaacattag ttatttacag ttacaggatg
     6181 ctgcctgata gaatctttat actggttttc catagaatcc tatcagacag gttaataaaa
     6241 cagtgcatat ccacaggcct ggctaccact ggttctccgg tgagcaaatt atgtggaaaa
     6301 cagtctagga atcttattat ttttagaacc ttgataaaag aagctaggat tagtcagtgg
     6361 aaaatcacat ccatcagggc aatgacccgg taataaagca tatactgaca tccgcctctt
     6421 ggataaactg aacgaaggaa tttaggacat ttgatcctgc gagacatgga agggtaagtt
     6481 accagagcga ttagcggaca tccaatggca accatattta tctagaacaa cacacatctt
     6541 atttaggttg ccccattgct cccatgtttt tcaataatta caaggtatat ccatgcggct
     6601 cctattgcat taagaggaat cctaagaatg agagagatat ttagagataa ttaaagatta
     6661 tagaagaagg aggatgaata gtggctgttc aaagaaatgc atctcaaata gtgttgcaaa
     6721 ttatttttac tatatttgtt tcgcttattg tattaatatc tttctatc
//
LOCUS       DASRNX010000140         4080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_506094,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000140 DASRNX010000000
VERSION     DASRNX010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506094"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1002)
                     /locus_tag="VFD89_05760"
     CDS             complement(52..1002)
                     /locus_tag="VFD89_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZJ57732.1"
                     /translation="MMELFGKFDENIRLLEEEFRIKIIVRQTQLRIIGGEKSVDMAFT
                     VLNRLLDGIGDGTRVDKGRVRYLIQLVKAGETDHIVELTGDVICVTYKGKQIKCKTLG
                     QTQYVKAMRRNDIVFGIGPAGTGKTYLSMAMAVNAFKNKEINRIILTRPAVEAGEKLG
                     FLPGDLQNKVDPYLRPLYDALHDLMGLDTYTRLIERGIIEVAPLAYMRGRTLDDAFII
                     LDEAQNTTSEQMKMFLTRLGIGSKAVINGDITQIDLPTGAKSGLVEAAEILNGVKGIG
                     FVYLTHRDVVRHELVMSIIRAYDNFHRKGQAPLETTGNTE"
     gene            complement(1065..2255)
                     /gene="yqfD"
                     /locus_tag="VFD89_05765"
     CDS             complement(1065..2255)
                     /gene="yqfD"
                     /locus_tag="VFD89_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HZJ57733.1"
                     /translation="MIFLKVWNYFLGYLVIKVDGLSLEKFINLCVSRGIKLWGIKRIS
                     YTSLTANIGIKDFKSLPPITRIVHSRIKIQNRKGFPFLLYHFRHRKMLLTGIIIFMMA
                     LYVLSSFIWQVEVVGKLKIGSEIIYSNLEKKGIRPGAYKGKLDTWKIENEMLIEIPEL
                     SWISIEFKGTRAIIRAVEGVKPPKLLDAKTPCNIIAAKDGIIDQLIVLEGDTIVEIGQ
                     TVRKGQLLVSGIIHHEDTEILRYVHAQAKVLARTWYEGKGNVDTGELSKARTGKKITH
                     KVLETGEWEIDINKKEIPFEEYEVEEKREPFLGLAKFGWISWSIRDYFEIKPVSRENA
                     EELAKERASAVAMEDAFKRIPKGAKIIDKKSKYYIIKDKGYEAVVYIEVLEDIAEQVG
                     LSTN"
     gene            complement(2359..2637)
                     /gene="yqfC"
                     /locus_tag="VFD89_05770"
     CDS             complement(2359..2637)
                     /gene="yqfC"
                     /locus_tag="VFD89_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfC"
                     /protein_id="HZJ57734.1"
                     /translation="MKKKKLQTVKSTVSEMFELPKEITLNMPKINLIGNNQMLVENHR
                     GIIEYTPSLIRVNSTVGVIRVQGNELNLRNIAAEDIMISGDIRSMEFI"
     gene            complement(3007..3408)
                     /locus_tag="VFD89_05775"
     CDS             complement(3007..3408)
                     /locus_tag="VFD89_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57735.1"
                     /translation="MVEILIFLTIASIIVNIIKAVGRAKTPQIRGANGETPPDPQGIN
                     PQQPSAEKTGPKYTIRMGSPDVGRPVVTEGGLKSDPPNTARRGKQRQIVPRTQPHTKG
                     MFDKLTRDRLIEGIILSEILAAPKSKRRGIS"
     gene            complement(3488..>4080)
                     /locus_tag="VFD89_05780"
     CDS             complement(3488..>4080)
                     /locus_tag="VFD89_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZJ57736.1"
                     /translation="IGIIAIIIEIFTKGFGIFGIVGISALALYFGANFIAGYSQWWPI
                     FLFLIGAILLGIEALIPGFGLPGISGISAMLIGVVFASSDPVKGVKNLGISSALALVI
                     TPALIYILIRTGTFKSLVLPDAIVGEPEIAKDNKTVELPGRRGVALTPLRPSGIISID
                     GVRFDAVTQGEYIASGTDIKVVQSRGLSVIVERDGV"
BASE COUNT         1038 a         1019 c          723 g         1300 t
ORIGIN      
        1 agaatctaca catcaagggt cctatcatta aaattcaata gtcatgctac ttcattcagt
       61 attaccggtt gtctcaaggg gtgcctgccc cttcctatga aagttatcat aggccctgat
      121 aattgacatt accagttcat gtctgacaac gtccctatgg gttaggtata caaagccgat
      181 gcccttaacc ccatttaaaa tctccgcggc ctcgaccaag ccggattttg cccccgtggg
      241 cagatctatt tgtgttatat ccccatttat tacagccttg gacccaatcc caagccgggt
      301 taaaaacatt ttcatctgct ctgaggtggt attctgggct tcgtccaata ttataaaagc
      361 gtcatccagt gttctgcccc gcatataagc caatggggct acttcaataa taccccgctc
      421 aattagcctg gtataggtgt ctaggcccat caggtcatgc aatgcgtcat acaaaggcct
      481 tagatatggg tcgaccttgt tctgtaggtc acccggtaaa aatcctagct tttctccggc
      541 ctctaccgcc gggcgggtca aaattattct gtttatctcc ttattcttaa aggcattcac
      601 cgccatggcc atggacaggt atgtctttcc ggttccggca ggcccaatcc caaacactat
      661 atcatttcgt ctcatggcct ttacatattg ggtttgtcct aaagtcttgc actttatttg
      721 cttaccttta taggtaacac atataacatc ccccgtaagc tcaacaatat gatcagtttc
      781 cccggccttt accagttgaa taagatacct taccctacct ttatctactc tggtaccatc
      841 ccctattccg tctaaaagcc tgtttagaac ggtgaaagcc atatcaactg atttttcacc
      901 gcctatgatt cggagttgag tctgtcgcac tattatcttt attctaaact cttcctccaa
      961 caatcggata ttttcatcaa actttccgaa aagctccatc atatggctca agttttgtat
     1021 atcaatcagt ctttccttct gtatttcctc caaataattt cctcctaatt ggttgaaagc
     1081 cctacctgtt ctgcaatatc ttccagcacc tctatataaa ctactgcttc ataacctttg
     1141 tcttttataa tatagtattt ggactttttg tcaatgattt tcgcaccttt ggggattctt
     1201 ttaaatgcat cctccatggc cactgcggaa gccctttctt tggctaattc ttcggcattt
     1261 tctcggctta ccggctttat ttcaaaatag tcccttatag accaggaaat ccaaccaaac
     1321 ttagctagcc ctaaaaaagg ctctcttttc tcctcaacct catattcttc aaagggtatt
     1381 tctttcttat ttatatctat ctcccattcc cccgtttcca acaccttgtg ggtaattttc
     1441 tttcctgttc tggcctttga aagttcccct gtatctacat tcccctttcc ctcataccag
     1501 gttctggcta ataccttggc ttgtgcatgg acataacgaa gtatttcggt atcttcatgg
     1561 tgtattattc cgctgactag gagttggcct tttctgacgg tttgacctat ctctactatg
     1621 gtatctccct ccagcactat aagctggtca ataataccgt cctttgctgc aataatatta
     1681 cagggtgttt ttgcatccaa aagtttaggt ggctttaccc cttccaccgc cctgatgata
     1741 gccctggttc ccttaaactc tatactgatc catgaaagct cggggatctc tataagcatt
     1801 tcgttctcta tcttccatgt atccagtttt cccttatatg ccccgggtct tattcctttc
     1861 ttttcgaggt tggaatagat aatttctgag ccaattttaa gtttccccac tacctccacc
     1921 tgccaaataa aggaggatag cacatataga gccatcatga atataattat accagttaat
     1981 agcatctttc ggtgcctaaa atgatatagc aggaagggga aaccctttct attctgtatc
     2041 tttattctgg aatgcactat cctcgtgatc ggcggcaggg acttaaaatc cttaatccct
     2101 atattggcag ttaaggatgt gtagcttatt cgttttattc cccatagctt tatgcctctg
     2161 ctgacgcata gattgatgaa cttttccagg gacaagccgt ccacctttat aaccagatag
     2221 cccaaaaaat aattccaaac ctttaaaaat atcaagatgc tcacccctag ccggcgtata
     2281 tttattcggg tgttatttta aaaaaacggg ataatccgct ccaatccatg ggctaaaccc
     2341 ggcgcaaggt tctcagccct atataaattc catagacctt atatcaccgc ttatcattat
     2401 atcttcggcc gctatattgc gtagattcaa ttcatttccc tgtaccctaa ttacccccac
     2461 tgtcgaattg actcttataa ggcttggggt atattcgata attcccctat ggttttctac
     2521 cagcatctga ttgttgccga tcaaattgat ctttggcatg ttgagggtaa tctccttggg
     2581 tagctcaaac atctctgaaa ctgtagattt aactgtctgt aacttctttt ttttcatata
     2641 aactacctct tgcaatgtta tttgaatatt gttaaaggcc gcccatacgg gggttgaacc
     2701 tatggaataa aattgtattt gctctatccg aattaaaggg tacccatacg ggggttgaac
     2761 aggactgatt aacgattttt tgccattaca cccagaatcg gtaaaatctg ttcccacaga
     2821 ctcatttttg ggccacatgg cgaacctttt catgacatat atagttaatt ctatgctaga
     2881 aataaaaaac tagaatgaat tcaccattct agttcttaca atggactatg ccttgacccg
     2941 gttatccggc ccatgtcctt taaaggcatc ttttcccctg ctatgtgatt ggggggatag
     3001 gcaaacttag gatatgcccc tccttttact ttttggagca gctaaaattt cagatagaat
     3061 aatcccctct atcagcctat cacgggttag tttgtcgaac atcccctttg tatggggctg
     3121 cgtcctgggt actatttgtc tttgttttcc ccttcgagca gtatttgggg gatcagattt
     3181 caaaccccct tctgtcacta caggccttcc gacgtcaggg cttcccattc tgatggtgta
     3241 tttcggaccc gttttttctg cgctgggctg ctgggggttg atcccttgcg ggtcgggtgg
     3301 tgtttcccca ttcgctcccc ttatctgcgg ggtcttcgct cgccctacag ctttaataat
     3361 gttgactatg atggatgcaa tcgttaaaaa aatcagaatc tcaaccatta tataaatcct
     3421 ccttaagact tcatcattaa acagctttta ttgtctgctg ttaaaggttg catgaaataa
     3481 attcaatcta taccccatcc ctttccacga tcacggatag accccgagat tgaaccacct
     3541 taatatccgt accggatgct atatattcac cttgggtaac cgcatcaaac ctaactccat
     3601 cgatacttat gatcccagag ggtctcaagg gagttagggc cacacctctt ctcccaggga
     3661 gttcaacggt tttgttatcc ttagctatct caggttcacc gacaatagca tccggtagaa
     3721 caaggctttt aaaggtgccc gtacgtatca gtatatagat aagtgcgggg gttataacca
     3781 aggctaatgc tgatgaaatg cccagattct taacaccttt cacaggatct gaggaggcaa
     3841 acacaacccc tatcagcatg gcgcttatcc cactgattcc cggaaggcca aatcccggga
     3901 ttagagcctc tatacccaaa agaattgcac ctatcaagaa cagaaagatg ggccaccatt
     3961 gtgaataacc cgcaataaag ttcgcaccaa aataaagagc aagggcactt atacccacga
     4021 ttccgaatat cccaaacccc tttgtaaata tctctataat aatagctatt attcctatgg
//
LOCUS       DASRNX010000141         4443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_187456,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000141 DASRNX010000000
VERSION     DASRNX010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187456"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFD89_05785"
     CDS             <1..996
                     /locus_tag="VFD89_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002134329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZJ57737.1"
                     /translation="GFFGGIGGASKKGILVKGGNFLEAMTDIDTVIFDKTGTLTKGVF
                     AVTQINPVPGVGEADLLHYAALAETHSTHPIAISILRAYSGKIDGNQIEDYKEIPGLG
                     ITVKTREYVILAGNEGLMKSNDIKYRSTNALGTIVYIAVDGKYMGNIVVSDELKKDAK
                     MAVTRLKGLGVKSLVMLTGDSRKVGEKVAEDLGLDGVYAQLLPHEKVEKLESIEKNKT
                     TGGRLVFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADIVLMTDEPHKLAEAIQIS
                     RKTKKIVLQNIVLALSVKGIVLLMGAMGIATMWEAVFADVGVALLAVLNSMRAVR"
     gene            1073..1999
                     /locus_tag="VFD89_05790"
     CDS             1073..1999
                     /locus_tag="VFD89_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HZJ57738.1"
                     /translation="MDTIKIKRLWSGFWQLADPKIWIASTIPMLVGASLSYSVTGKFD
                     PLWFVLSLMGIYSIEIGKNSINEYIDYRSGVDLNIAPENRTPFSGGKKTVVQGRLTLK
                     EVLLITLGTFAFACGIGLLIVAFREPGVLWVGLLGVLVSIFYSVPPLKFAYRGLGEFA
                     VGFTFGPLITLGIYLVMSGTFDFKVMVAGLPLGFLIANVLWINQYPDYEVDLKGNKRN
                     WVVRLGKKRGILIYALLFILAYASFIPVAVLYKNPVWLIGWISIPLAAKSTLIAGKEF
                     NNIPKLIRANAFTIFIYQVTGLAMIVASLVMK"
     gene            2070..3191
                     /locus_tag="VFD89_05795"
     CDS             2070..3191
                     /locus_tag="VFD89_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57739.1"
                     /translation="MGIKGKVRRVFPGGNTPTGFHSFFDHITGKNYTRRYIIKGGPGA
                     GKSTFMAKIGEHMLNLGFDIDEYRCPTDPDSLDAISIPKLGIALLDGTSPHVVDPKNP
                     GITDDIIWLGRYWDDARLAESKDEILKLQKKGGRLFKSAFSRLKEAKVAYDEWRSYAE
                     DDLNENEYHTVIRQAMSDLFKGYKGRPHEETYHNHLFASAISGKGVRNYTGGLIEPDH
                     RVYAFSGRPGSGVGKAIDRLAREAGELGLSTQRFHSPIDTDELDILIIPQLNRVLVNV
                     NQTIGNDNSWLDDRPVERHIDFDRCLGNVTIREFADEIEAAKMRFYNLIEGAVNFISG
                     AGEIHSQLEKYYIEAMDFEKVNSKREEVLREILKISEIS"
     gene            3252..4256
                     /locus_tag="VFD89_05800"
     CDS             3252..4256
                     /locus_tag="VFD89_05800"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HZJ57740.1"
                     /translation="MPRLMNTRIVIGNNLDPWRNLAMEEYLLEDVKPKECILYLWQNQ
                     NTVVIGKNQNAWKECRIRLLESEGGKLARRSSGGGAVFHDTGNLNFSFLVDKGFYDES
                     RQLEVVLDAVNDMGIKAVFSGRNDLTVDGKKFSGNAFCFRKNSALHHGTILIAADMDK
                     LTRYLEVPEDKIRSKGIESIRSRVVNLREYYPKLDVPSMIQALIRNFGRVYGQTQDIS
                     YGEEGFDQNLLNKLNEKYKSWEWRFGESPKFDIELNTRFPWGGIHLGLELKQGKISKA
                     HIYSDAMDEALIEMIPDIIQGCLFNSPNIADSLAEVKVGAKRGEMLRDIADWIRSKGF
                     "
BASE COUNT         1304 a          814 c         1185 g         1140 t
ORIGIN      
        1 ggtttttttg gcggaatagg cggggcatcc aaaaaaggta ttttagtgaa gggcggaaac
       61 tttcttgaag cgatgactga tatagatacg gtaatctttg acaaaacagg cacattgact
      121 aaaggggttt ttgccgttac acaaatcaac ccagtccccg gtgtcgggga ggctgacctg
      181 ttgcattatg cggcattagc ggaaactcat tcaactcacc ccattgccat atctattttg
      241 cgggcctata gcggtaaaat tgatggtaat caaatagagg actataagga aattccgggg
      301 ctcggcataa ccgttaaaac acgggaatat gttattttgg ccggaaatga agggctgatg
      361 aaatccaacg atatcaaata cagatcaact aatgcccttg gaaccatagt atatatcgcg
      421 gtggacggaa agtatatggg taatatcgta gtatccgatg aactcaagaa ggatgcaaaa
      481 atggctgtaa caaggctcaa gggcctaggg gtaaaatctt tggttatgct taccggagat
      541 tcaaggaagg ttggcgaaaa ggtagccgag gatctgggat tggacggggt atacgcgcaa
      601 ctgctgcccc atgagaaagt agaaaaattg gaatctatag aaaagaacaa gaccaccggt
      661 gggaggctgg tcttcgttgg ggatggtatc aacgatgcgc ccgtactggc cagggctgat
      721 gtggggatag ccatgggagg tttgggatct gatgcggcta tagaagcggc ggatatagtt
      781 ctgatgacag atgaacccca taagttggca gaggccattc agatatccag aaaaacgaag
      841 aaaatagtat tgcaaaatat tgtattggcc ctctcggtca agggaatcgt attgcttatg
      901 ggcgccatgg gtatagctac aatgtgggag gcagtttttg ctgacgtagg ggtagctcta
      961 ctggctgtgt taaattccat gagagcagtc cgttgacatc tgtaatgcaa aagcccttcg
     1021 gggctattgg catatgttcc gattatatat tatgaaaatg gagaattgga ttatggatac
     1081 cataaagata aaaaggctat ggagtggatt ttggcaactg gcagatccaa agatatggat
     1141 cgcatccacg atccccatgt tagttggggc atcattatcc tatagcgtaa cggggaagtt
     1201 cgaccctctg tggttcgtcc tatcgttgat ggggatctac tcaatagaga tagggaaaaa
     1261 ttctatcaat gagtatatag attatagatc gggtgtagat ctaaacatag ccccagaaaa
     1321 cagaacaccc tttagcgggg gtaagaaaac cgttgtccag gggagactta ccctaaaaga
     1381 agtcctgctt atcaccttgg ggaccttcgc ctttgcttgc ggtatcggcc tgttgatagt
     1441 ggcgtttagg gaacccggcg ttttatgggt aggcctgtta ggggtccttg tatccatatt
     1501 ctatagcgtt cctccgctaa agtttgccta caggggattg ggggaatttg ctgtggggtt
     1561 tacctttgga cccctaataa ccctgggtat atatttagtt atgtctggga cctttgattt
     1621 taaggtgatg gtagccgggc ttcccttggg ttttcttatt gccaacgtat tatggatcaa
     1681 tcaatacccc gattatgaag tcgatttgaa gggtaataaa agaaactggg tcgttaggct
     1741 ggggaagaaa aggggcatcc tgatttatgc tttgctgttt atattggcgt acgcttcatt
     1801 tatcccagta gccgtgttgt ataaaaatcc tgtttggcta attggatgga ttagtatacc
     1861 attagctgca aaatctactc ttatagcagg aaaggaattt aataatatac caaaactgat
     1921 tagagccaac gcatttacaa tttttatcta tcaagtaacc ggattggcta tgattgttgc
     1981 ttcccttgtt atgaagtagg gccttccaaa attcccccac cggcatatca tatagagtaa
     2041 gaatatgctt atggaaggag acgaaacgta tgggtattaa aggtaaggtg agaagagtat
     2101 ttcccggtgg gaatacccca acgggattcc attccttttt tgatcatatt accgggaaga
     2161 actataccag gcgctacata ataaaggggg gtccgggtgc agggaagtcc acgttcatgg
     2221 ccaaaatagg ggaacacatg ctgaatctgg gctttgatat agatgaatac cgatgcccaa
     2281 cggatccgga ttctctggat gccatttcta taccgaaact ggggatcgca ttgttagacg
     2341 ggacctcgcc ccatgttgta gatccaaaga acccgggcat aaccgatgat ataatctggc
     2401 tgggacggta ttgggacgat gctagactgg ccgaatccaa ggatgagatc ttgaaactcc
     2461 aaaaaaaggg cggcaggtta tttaaatcgg cctttagtcg tcttaaggaa gccaaggtag
     2521 cctacgatga gtggaggtca tacgctgaag acgacctcaa cgaaaatgaa tatcatactg
     2581 ttattaggca ggcgatgtcc gatctcttta aaggatacaa gggtcgaccc catgaggaga
     2641 cgtatcataa ccatttgttt gcatcggcta ttagcgggaa aggagtgcgc aattataccg
     2701 gtggcctgat agaaccggat cacagggtct atgctttttc gggcaggccg ggatcggggg
     2761 tagggaaggc tatcgatcga ttagctcgag aagcggggga gctggggcta tctacccagc
     2821 gatttcattc acccatagat accgatgaat tggatatact aattattccg cagctgaata
     2881 gggtactggt caacgtgaac cagaccatag gcaacgataa ttcgtggttg gacgataggc
     2941 cagttgaaag gcatatagac tttgatcgtt gtttgggaaa tgtaactatc cgggaattcg
     3001 cagacgagat agaggcggca aaaatgcgtt tttacaacct gatagagggg gcagtgaact
     3061 ttatatccgg ggcaggtgag atccatagtc aattggaaaa atactatatt gaggctatgg
     3121 actttgaaaa ggtcaattct aaaagggagg aagtattgag ggagatactg aaaatatccg
     3181 aaatctcata ggggattttt tacagaaggg gggaacgggc atttcctaca cgatggaatc
     3241 cgtgagggct tatgcccaga ctaatgaaca cgagaatagt tataggtaat aatctagatc
     3301 catggcgcaa tctagcgatg gaagaatatc tgttggagga cgtaaaaccc aaggaatgta
     3361 tcctctatct gtggcagaac cagaacaccg tagttatcgg gaagaatcaa aacgcttgga
     3421 aagaatgtag aattagactg ttggaatcag aggggggaaa gctggcacga aggagttccg
     3481 gcggtggggc ggtttttcat gacaccggca atcttaactt ttcattttta gtggataagg
     3541 ggttctacga tgaaagcaga caattagagg tcgtcctaga tgccgtgaat gatatgggta
     3601 taaaagccgt ttttagcggt agaaatgacc tgacggttga tgggaaaaag ttctcgggaa
     3661 acgcattttg tttccgtaaa aattctgctc tacaccatgg gactatactc attgcggcgg
     3721 atatggacaa attgaccagg tatctggaag tgcccgagga caagatccgt tcaaaaggta
     3781 tcgaatccat aaggtcaagg gtagttaacc tacgtgaata ctatccgaaa ttggatgtac
     3841 ccagtatgat ccaggctcta ataaggaatt ttggacgggt atacgggcag acccaagata
     3901 tcagctatgg ggaagaggga ttcgatcaaa atctattaaa taaactcaac gaaaagtaca
     3961 aatcctggga atggcggttc ggggagtcgc ctaaatttga tatagaacta aatacgcggt
     4021 ttccatgggg gggaatccat ctggggttag aactgaagca gggcaaaata tccaaggccc
     4081 atatatattc cgatgctatg gatgaggctc tcatagagat gattcccgat ataatacagg
     4141 gatgcctgtt taattcccct aacattgccg attctctggc tgaggtaaag gtaggtgcaa
     4201 aaagggggga gatgttgcga gatatagcgg attggattag aagcaagggc ttttagaccc
     4261 aaaaaggccc ggttcattta gtcgtcccgg gttttaccat agactgctga atcagcatca
     4321 gaacgcctgc agacagtatt atatctccca ggcttataaa ttggcgcaag gggaaggaaa
     4381 tccggatagt atcacctaaa aaccaaagta agacaccggg ctctataagt ttatatgaat
     4441 aaa
//
LOCUS       DASRNX010000142         5927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_135904,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000142 DASRNX010000000
VERSION     DASRNX010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135904"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..752)
                     /locus_tag="VFD89_05805"
     CDS             complement(273..752)
                     /locus_tag="VFD89_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZJ57741.1"
                     /translation="MISDVDLMHLRRCVELAEIALDKGDEPFGSILVSANGDVLFEDH
                     NHVAGGDHTRHPEFALARWAAANMTPEERSRATVYTSGEHCPMCAAAHGWVGLGRIVY
                     ASSSKQLVQWLDEMGANPSRVRNLPIQDVIRDTVVDGPVPELAEKIRKLHMSRGRDK"
     gene            complement(951..1808)
                     /locus_tag="VFD89_05810"
     CDS             complement(951..1808)
                     /locus_tag="VFD89_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZJ57742.1"
                     /translation="MGTPKYKVSRIKTIDDAVIEYVHIKGDGTPIILIPGAGDGLSMV
                     GKFPNPYTLARMFKGYAIKHNIIIMSRREPIPKGYTVRDHARDYIWAMDELGIDKAHI
                     ETNSAGGPIGQWIAIDAPDRVRSLVLGASMAHTDRHLNVILRQWIEWSKDGEWYKLNL
                     DSTIRTYTPRYYRKYRWLFPLLRLLPKPNNPKRVVRIFEGLLDFDNKPYLNKITCPTL
                     VIGGDADEITSLKLQTEMARLIPDSKQIIIPGVGHGASQEANREHEEKVLLFYENVDK
                     SSKRETLVK"
     gene            complement(1993..3051)
                     /locus_tag="VFD89_05815"
     CDS             complement(1993..3051)
                     /locus_tag="VFD89_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZJ57743.1"
                     /translation="MGNLDSREYRGDKITLGSTGITTPRNAFGALPIQRVNVETSVMI
                     LRKAYEGGMTFFDTARGYSDSEEKLGLAFDGMRERIFIATKTGAKTPEGFWKDLETSL
                     TLLKTDYIDIYQFHNPDVCYRPDDGTGMYECMLEAKKQGKIRHIGMTNHRLNLANECI
                     DSGLYETLQFPFSYLSSDKDVDLINKCKEANMGFIAMKALSGGLITDSAAAYAFMAQY
                     DNVLPIWGIQRESELDEFLSYIENPPELTDERRALIDEDKDELIGEFCRACGYCMPCP
                     MGIEINICARMSLLLRRSPSDNWLTPRMQEAMARIEDCTECGQCMEKCPYDLDTPNLL
                     KKNYEDYKKVLAGEVNVS"
     gene            complement(3163..4029)
                     /locus_tag="VFD89_05820"
     CDS             complement(3163..4029)
                     /locus_tag="VFD89_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZJ57744.1"
                     /translation="MRTRRLPFYECRPGIFEIDEFDCGSIFVIVGDERALVMDTGTGI
                     GDLRWVIENKITDKPYDAVITHSHGDHIGGAGFFDSVWVHANDMDWDNEKFMPRLDFR
                     KRYATLIADREGKHYSYDIERDIVEWGKMPHKEELKDGQVFDLGGRSVTVYHCPGHTP
                     GQCVAIDDLTATLLCGDACNNNLLICKESGKTDLESIETAYRSLNRIWEMKDSYEEVY
                     NFHHDYRGFGSPLAENVLPNAVECLRRLVDGKAELKMVPDALSDTGAEKMVAVFDNVQ
                     ITYMGGDISKLR"
     gene            complement(4204..4578)
                     /locus_tag="VFD89_05825"
     CDS             complement(4204..4578)
                     /locus_tag="VFD89_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57745.1"
                     /translation="MKYRGRLVRRLHTPFHRDGDFCLSSSQMLGQDPLIQPKMTARSI
                     SGKTPFLLIENDCMIVTGDSLLNAFDRLEVAEYSVKTITDSAIHGNIVAVNDDEIAIL
                     NGPLTFKFREADNEGHYVCRHF"
     gene            complement(4717..5775)
                     /gene="rsgA"
                     /locus_tag="VFD89_05830"
     CDS             complement(4717..5775)
                     /gene="rsgA"
                     /locus_tag="VFD89_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZJ57746.1"
                     /translation="MILKKYGFNPDILSSLNVSETETIADVARVIAVHKERYELVCEY
                     GGTFGRLKTGVYYAGAYESFPTTGDFVLINYNPIGDSQIIKTLPRRSCFTRRDPTPGR
                     GEQSVASNFDYVFIMQSLNLDFNIKRLERYTTLAWQSGALPVIILTKVDLVQDFSQQV
                     REAEKVAPGVSVHPVSAVNGYGIDALAYYLEPQKTIVFLGSSGVGKSSLVNALAGNDI
                     MEVKEIREDDSRGRHTTTHRQLIMLPGGAMIIDTPGMRELGMWDVSRGLPEAFEDVEQ
                     YFGLCKFSDCKHQREPGCAIRAAIENGDLPPGRWDSYISLRREAEYSDDRASYLRQKQ
                     QRHKEIAKWSKQNKKNGK"
BASE COUNT         1481 a         1551 c         1119 g         1776 t
ORIGIN      
        1 taattatact ctgccgtgca taccggtgga atcggcgaaa gacgtcaggc aaaagatgaa
       61 atgttccggc aaaaatatca tctgccccat ccccttgaca tatggggtac agaagaaatg
      121 tgccggtaat aatatatcga acaccccgtc ctcccggcac gtttatttat tggatattcc
      181 gtccgctcgg tatgtacatt ccactaacat gagtcactta ccccgtcccc ttgacacgcg
      241 tccccttgac acgtccattc cactaacatg tgtcacttat cccgtcccct tgacatatgg
      301 agctttcgga ttttttcggc aagctcggga acgggcccat cgactacagt atcgcgaata
      361 acatcttgaa tgggcagatt cctaacccgc gacggattag cccccatttc atccagccat
      421 tgtaccagct gcttggatga acttgcataa acaattctgc cgagacccac ccaaccatgg
      481 gccgcagcgc acatagggca atgctcaccg gatgtatata ccgttgccct gcttctctct
      541 tcgggtgtca tgtttgcagc tgcccacctc gccaatgcga attccggatg tctggtatga
      601 tcgccaccgg caacatggtt atggtcttcg aagagcacat ctccattcgc agagacaagg
      661 attgaaccga agggttcatc gcccttatcc aaggcaattt cagctaattc aacgcaacgc
      721 ctcaggtgca ttagatctac atcgctaatc attttataat cccctccaac aaataactca
      781 ggttaattcc caatgcgtga tttaaattgt tctgatagct ccaattcata actctatcac
      841 acacgttact attgtacccc gtactatcgt atcccgctgc tatcacccct tgttactata
      901 agatcatatt ctgccaataa tatgtagtgt tttacattta ctggacctat ctattttacc
      961 agtgtttccc gtttactgga cttatctaca ttttcataga acagcagtac tttttcttca
     1021 tgctccctgt ttgcttcttg gctggcgccg tgaccgacac ctggaataat gatttgcttt
     1081 gaatctggaa ttaggcgggc catttcagtt tgcaatttta gacttgttat ttcgtcggca
     1141 tccccaccta ttactagggt aggacacgtt attttgttca aataaggttt gttatcaaaa
     1201 tcaagaagcc cctcaaatat cctgactacc ctctttggat tattgggttt cggtaagagc
     1261 ctgagaagtg gaaatagcca cctgtacttt ctataataac gtggtgtata ggttctaatt
     1321 gtgctatcta aattcagctt ataccactcc ccatccttgc tccattctat ccattgccgt
     1381 aagatcacat tcaaatgcct atcagtatga gccatactgg cgcctaacac gagactcctt
     1441 accctatccg gggcatcaat tgctatccat tgccctatgg gcccaccggc ggaatttgtt
     1501 tctatatgtg ccttgtcaat tcccagctca tccatcgccc aaatataatc cctcgcgtgg
     1561 tccctgactg tatatccttt tggaataggt tctctcctgc tcatgatgat aatattatgt
     1621 ttgattgcat accctttaaa catccttgca agggtgtacg gattagggaa ttttccaacc
     1681 atgctaaggc catctccagc acccgggatt aggattatcg gtgttccatc cccttttata
     1741 tgcacatatt cgataacagc atcatctatc gtctttatcc gtgatacctt atattttgga
     1801 gtacccaccg ataatcacac ccattcatta attgttaatc gtccataggt ccatgttatt
     1861 attgataata tttgccggca tttgccgttc tgtccgcttt tagtttagct gtcattttat
     1921 cgttccagtg tcgccgatag caccgtatta tcgtacttcc ccttttgtcc cgtgtgtcgc
     1981 tgaccccgtc ccctatgaca cattcacttc gcctgccagc accttcttgt agtcttcata
     2041 gtttttcttt agcaggttgg gggtgtcaag gtcgtagggg catttttcca tacattgacc
     2101 gcactctgtg caatcttcaa ttcgtgccat ggcttcctgc atcctaggcg tcagccaatt
     2161 gtcagatggt gagcgtctga gcaaaaggga catcctggca cagatattaa tttcaatccc
     2221 catggggcaa ggcatacaat aaccgcaggc cctgcaaaat tcccctatta gttcatcttt
     2281 atcttcatcg atcaatgctc ttctttcgtc cgttaactct ggtggatttt caatataact
     2341 taaaaattca tccagttccg attcacgctg gattccccat atggggagca cattatcata
     2401 ctgggccata aatgcgtatg ccgccgcaga atccgtgatc agtcctccgg acaatgcctt
     2461 catggctata aaccccatgt tagcttcctt gcatttgttg ataaggtcaa catccttatc
     2521 tgacgataaa tagctaaagg ggaactgtag ggtttcgtat agcccagaat ctatgcattc
     2581 attggccaga ttcagcctat ggtttgtcat accgatatgc ctaattttgc cttgtttctt
     2641 ggcctccagc atacattcat acatccccgt cccgtcgtcg ggcctatagc aaacatccgg
     2701 attatgaaat tggtaaatat ctatataatc cgtctttaaa agggttaacg aagtctcaag
     2761 atccttccaa aacccttccg gcgttttagc ccctgtcttg gttgcaataa atattctttc
     2821 ccgcatccca tcaaaggcta accccagttt ttcctcactg tccgaataac cccgagcggt
     2881 atcaaaaaag gtcatacccc cctcataagc ctttcttaaa atcatgaccg aagtttccac
     2941 gtttaccctt tggatcggca gagcaccgaa cgcattgcgt ggggtcgtga taccggtgct
     3001 tcccaatgtt attttatcgc ctctgtattc cctggagtct aaattaccca taccttccac
     3061 cctttcacta taatttttaa agagatcctt acaattaatc ttgcgtccaa cagacatatt
     3121 tcctttatga tttaacggag tctactgata tccccttata atttaacgaa gtttactgat
     3181 atccccgccc atataggtta tctgtacatt atcaaatacc gctaccatct tttcggctcc
     3241 tgtatccgac agggcatccg gtaccatttt tagttccgcc tttccgtcta ccagccgcct
     3301 taggcattct actgcattag ggagaacgtt ttcagccaat ggagagccaa acccccgata
     3361 atcgtgatga aagttataga cttcctcgta gctgtccttc atttcccata ttctgtttaa
     3421 tgatctgtat gcggtttcta tggattctaa gtcggtttta ccgctttcct tgcatattag
     3481 aaggttattg ttgcatgcgt ccccgcacag cagggtagcc gtcagatcat caattgctac
     3541 gcattgcccc ggcgtgtgtc ccggacaatg ataaacagtt acgcttcgtc ctccgagatc
     3601 gaacacctgt ccgtccttta attcctcttt atgaggcatt ttcccccatt ccacgatatc
     3661 cctttcgata tcatagctgt aatgcttacc ctccctgtcg gcgataagcg tcgcatacct
     3721 ctttcgaaaa tccaacctag gcataaactt ttcgttatcc caatccatat cgttggcatg
     3781 tacccaaacg gagtcaaaaa aacctgctcc gccgatatgg tcaccgtggc tgtgtgtgat
     3841 aacggcatca tagggtttat cggtgatctt attctcaatt acccagcgta ggtcccctat
     3901 tcccgttccg gtgtccataa caagggccct ttcgtcaccg acgatcacaa aaatacttcc
     3961 acaatcaaat tcatcaattt cgaaaatgcc cggcctgcat tcgtaaaacg gtaatcttct
     4021 ggttctcatt taacttcctc cctataatta aaattctctg gcggtaataa gcctaaaata
     4081 tgccggcaga tatgcagtaa caagctcaaa aaaccgccag tagatatgca gtaacaatca
     4141 aaaatcatgc cggcaaacac acagtaatgg gttcaaaaac gcaacagatg cgcagcaacg
     4201 agcttaaaaa tgacggcaga cgtaatgtcc ttcattatcc gcctcccgaa atttaaaggt
     4261 caaaggccca ttcaatatag ctatttcatc gtcgttaacc gctactatat tcccgtgtat
     4321 ggcggagtct gtgatagtct taacgctgta ctcggcaacc tccaatctgt cgaaggcatt
     4381 taggagcgag tcaccggtga caatcataca atcattttct atgagcaaaa agggcgtttt
     4441 cccggaaatg gatctggcgg tcatcttagg ttgtattaaa gggtcttggc ccagcatctg
     4501 ggaagatgat agacaaaaat ctccgtcccg atgaaacggt gtgtgcaatc ttcgaactaa
     4561 ccgtccccga tatttcaata ttaccccttt tttctttcct cttcaaccca aatagtctct
     4621 tttgatgcaa caacagatct attttgcctt atgggaacac tttttgccgc tataggcgga
     4681 ccgattaatc ggcaggggct ttaacctatc aggatcttat ttcccgtttt tcttgttttg
     4741 cttgctccac tttgcaatct ccttatgccg ttgctgcttt tgtctcagat aggaagccct
     4801 gtcatccgaa tactcggcct cacgtcttag gcttatatag ctgtcccatc tccctggggg
     4861 cagatcccca ttttcgatag cggccctaat tgcgcatccc ggctccctct gatgcttaca
     4921 atcactgaat ttgcataatc caaaatattg ctccacatcc tcaaatgctt ccggaagtcc
     4981 ccggcttaca tcccacattc caagctcacg catacccggg gtgtcgatga tcatagcacc
     5041 tccgggcagc atgataagct gcctgtgggt ggtggtgtgc cgacccctgc tgtcgtcttc
     5101 gcgtatttcc tttacctcca ttatatcatt tcctgccagc gcattgacga ggctagattt
     5161 accgacaccc gacgatccaa gaaatactat ggttttttgg ggttctagat aataggccaa
     5221 ggcatcgatc ccgtatccgt ttactgcgct tacaggatgt accgaaacgc cgggggcaac
     5281 cttttctgct tctcggacct gttgggaaaa gtcttgaacc aagtccacct ttgtcaggat
     5341 aattacaggc aacgccccgg attgccaagc cagggtggtg tagcgttcca gccgtttaat
     5401 attgaaatct aaattgagtg actgcataat aaatacataa tcaaagtttg acgcaacaga
     5461 ttgttccccg cgacccggtg tcggatccct cctcgtaaag catgacctgc ggggcagggt
     5521 tttaataatc tgactgtccc ctatgggatt atagtttatg agcacaaaat cacccgttgt
     5581 cggaaaactt tcgtaagcac cggcgtaata aacgcccgtt tttagtctcc caaaggtccc
     5641 gccatattca catacaagtt catatcgttc cttatgaacg gctataaccc ttgctacatc
     5701 ggctatagtc tctgtttcag acacgtttag cgaagaaagt atatcaggat taaatccata
     5761 tttttttagt atcaattctc aaatcctcca atttaattaa tattcatgat catggggcaa
     5821 ataactgaca aagtaaggtt tcaaaaaagt caggttgtcg gtatcggtat cgtgaacaaa
     5881 atcatctgat aacacatttt ttgaacctcc gctattgaaa taagtta
//
LOCUS       DASRNX010000143        12921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_295398,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000143 DASRNX010000000
VERSION     DASRNX010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12921
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295398"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /gene="pknB"
                     /locus_tag="VFD89_05835"
     CDS             complement(<1..1030)
                     /gene="pknB"
                     /locus_tag="VFD89_05835"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HZJ57747.1"
                     /translation="MLGKILGGRYELVEKIGTGGMAIVYKAKCRLLNRFVAVKILRPE
                     LVEDEEFVYRFKRESQAAASLSHHNIVNVYDVGQEDGIYYIVMEYVWGKTLKEFIREK
                     GNLESKEAIRIALQIAAALEHAHENGIIHRDIKPQNILIGNDYTIKVADFGIARAVTS
                     ATVTLAGPSVIGSVHYFSPEQARGGYIDAKSDLYSLGIVLYEMVTGTLPFEGDTAVSI
                     AIKHIQEMVKPPGEINPRIYKSLQAVIQKSISKLPEERYQNAGEMIKDLRRAAEEPNG
                     DFVVANYNSDAPTQVLKPISIDNHREVGGAISEPVISDNNPKKRPWIKAIVMILVFAM
                     IMGLAFYIG"
     gene            complement(1036..1794)
                     /locus_tag="VFD89_05840"
     CDS             complement(1036..1794)
                     /locus_tag="VFD89_05840"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HZJ57748.1"
                     /translation="MILEYGGYSHKGMVRKSNEDYYYIPSADKIQDLAMIADGMGGHN
                     AGELASRMAVETAIEYLTHRIDRDGYSQDPAGLIEEAIGVSNGRIFDVSLANEAYNGM
                     GTTLITAYFSHEILYLGHVGDSRGYLIRGGSVKQLTKDHSLVQELLDNGTITPDEVDK
                     HPQKNIITRALGTENVIDIDCHKIALKENDIILLCTDGLILHVDLQESIDLVESAHSM
                     QELALGLVDQAVERGGLDNVTVVAIKYISKKKEG"
     gene            complement(1857..2918)
                     /gene="rlmN"
                     /locus_tag="VFD89_05845"
     CDS             complement(1857..2918)
                     /gene="rlmN"
                     /locus_tag="VFD89_05845"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZJ57749.1"
                     /translation="MDILDKSPAEIVEILKQIGEQGFRGKQVVNWLYKGIEDFDDMTN
                     LPMTVRTKLRERYVIGRVNQMEKIVSDKGDTIKYLCLLHDDNIIEDVVMGYDYGNTLC
                     LSTQVGCRMGCSFCESTRQGLIRNLEVGEMVAQVLMANTDMGTVGTGERNIRNIVLMG
                     SGEPLDNYDNVVKFLRLIHDPETLNIGYRNITLSTCGLVPGIRRLADEDMPITLAISL
                     HAPNDKLRRRIMPAASAYGILEIVEAAKYYFERTGRRVSFEYSLMDGINDGSDEAMEL
                     GKLLGDFPCHVNVIPINTIEGAPYNRSPEARIQLFEKNLRSAGIQVTRRRELGQDIGG
                     ACGQLKSGYLNDFDGESFS"
     gene            complement(2930..4273)
                     /gene="rsmB"
                     /locus_tag="VFD89_05850"
     CDS             complement(2930..4273)
                     /gene="rsmB"
                     /locus_tag="VFD89_05850"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HZJ57750.1"
                     /translation="MDRVRKTALIILKDVNEEGKYANISLKENLNRISFQDRDASFVT
                     RLVYGTLENQAFIDNVLRKFAKMRGVNPWVENILRLGCYQILFLDRVPDSAACNESVK
                     LCVGFGFGSLKGFVNGVLRNISRHKKELMASEPIPTDTKSLASLYGFPLWLVDLWAGE
                     YGVDVARAIMESTIGANYITLRINKARISRDGLINELKQQGIKAAESPYFNNALRVEG
                     IGNIQANPLYKKGMFTVQGESSMLVSQILTPKPGECILDACSAPGGKAIYIAELMGMT
                     GKVYARDIHEHRVGLIDKSIERMGITNIETGVQDAGEFNPKMEQSMDRVLIDAPCSGW
                     GVLHKKPDIRLRIEERDMESLYRLQREILSTCRRYVKPGGTLVYSTCTINPWENHKAI
                     EGFVEKYPEFVLEDFNRELPMGLKDATIGEGMIQLFPHRHGVDGFFIAKMRRSAL"
     gene            complement(4321..5010)
                     /locus_tag="VFD89_05855"
     CDS             complement(4321..5010)
                     /locus_tag="VFD89_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HZJ57751.1"
                     /translation="MPFLFYDSTMLILLPALILSFYAQYKVQSTFNRFIRVANYGGYT
                     GAEVARKILDTMGLADIPVNRTGGHLTDHYDPRKKVLRLSDAVYNGRSIASIGVAAHE
                     AGHALQHANGYAPLSIRNAIVPVANFGSHGAFLFIFLGLVMGATVMLDIGIYLFLGVV
                     AFQLITLPVEFNASSRAVELLSIQGFVTREEEGNVKKVLNAAALTYVAAAASAVAQLL
                     RLLLIRDRRRR"
     gene            complement(5231..6178)
                     /gene="fmt"
                     /locus_tag="VFD89_05860"
     CDS             complement(5231..6178)
                     /gene="fmt"
                     /locus_tag="VFD89_05860"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZJ57752.1"
                     /translation="MKIIFMGTPEFSIPSLNAIISEGCELIGVVTQPDRPRGRGKRLL
                     PSPVKAWAMEKGIKVFQPSRARDEGFIVQITDLAPDLIITAAYGQILPKTILDIPSLG
                     CINVHASLLPKYRGASPIQQAVMDGETQTGITIMYMDVGMDTGDIILQREIPIHPGEN
                     SGDLHERLADLGGQALRESLRLFAEGKPVGIPQVHEEATYCQKIDKAFGKIQWDMDID
                     RIRNHIRALTPWPGAYTHIDGIRLKVWEAGQGEYFELKDIVPGQVVHADEENGLVISG
                     SNGLIRLFKVQAPGKRVMEDVEFLRGNPIKVKTLLDAED"
     gene            complement(6185..6670)
                     /gene="def"
                     /locus_tag="VFD89_05865"
     CDS             complement(6185..6670)
                     /gene="def"
                     /locus_tag="VFD89_05865"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZJ57753.1"
                     /translation="MALREIKHYERDDVLRKKARPVEKIDKRILLLLDDMAETMYNAE
                     GVGLAAPQVGILRRVAVIDIGDGIIELINPEIVERQGEQADMEGCLSIPGLTGIVKRP
                     DKVKIVGLNRDGEKIELEGEALLARALCHEIDHLDGILYIDRIEPGTLKRVDQETENR
                     G"
     gene            complement(6772..9033)
                     /gene="priA"
                     /locus_tag="VFD89_05870"
     CDS             complement(6772..9033)
                     /gene="priA"
                     /locus_tag="VFD89_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZJ57754.1"
                     /translation="MSDKLYAGVIVDQAHPAIDKIYHYEIPEDLKAGIQIGMRVLVPF
                     GAGNRNIDAYIMGLNKEIFIPHNRVKPIKEIRDPYPVLLPELVPLVWWMKKEYHCLTI
                     EAIRCFIPPGLRRNMDGKVEKMVFLKVETNIDEKIAQVESRSRYMAEILRILNEGDGI
                     SLPELADMAGGAPLSSFKNLEKRGWIFLEDQEVYRNPWDQPIDVLETELEPTKEQAIA
                     IETIGEGISMSQGIYLLHGVTGSGKTEVYMHLVEKTLKNRKRAIILVPEISLTPQTVG
                     HFKKRFGADVAILHSRLSTGERFDEWRRIYNRQVNIVIGARSAIFAPLQDLGLIVIDE
                     AHDDSYKSESRPRYHTIGLAAQRSKEQGAVLVLGSATPSLDDFYNSEKGLYTRVTLDH
                     RINYRPLPGVEIIDMRLEIKRGNRSIFSQKLYDELQGALSRGEQAIILLNRRGYAQFV
                     SCRSCGYVVKCNNCDISMTYHSKSNVLKCHYCGNTKPYPRICPECKSTYIKHFGVGTQ
                     KVEQEIGRLLSSARIIRMDMDTTSTKGAHQRILDAFRHREYDILLGTQMVAKGLDFPN
                     VTLVGVVTADTSLNLPDYRSSEKTFQLITQVAGRAGRGSREGKVIIQTYHPDHYAIQY
                     SSKHDYLGFYNEEIKIRNQFKYPPFSHIVRVLITGEIEDNVIKTALDIKKWMEYKGAE
                     DPIIKSGTIELGAYPAPLERIKNRYRWHVLARIRNERIFIERYHEIVDDCQREFFNAA
                     GTIVVDFYPTSLL"
     gene            complement(9152..10351)
                     /gene="coaBC"
                     /locus_tag="VFD89_05875"
     CDS             complement(9152..10351)
                     /gene="coaBC"
                     /locus_tag="VFD89_05875"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZJ57755.1"
                     /translation="MNSAFAGKNILIGISGGIAAYKAADLTSAMIKLGAGVKVVMTEN
                     ATKFIHPLTLESLSGNQVYHDTFHTTDRYDIEHIALAKWADVFIIAPATANVIGKAAG
                     GIADDLLSTTILATDAKTIFAPAMNTVMYQKGVVQDNIKKLKALGYTFIPPGSGRLAC
                     GDWGEGKLADVDDILKVLEDNLVENRDLIGKRVLVTAGPTIESIDPVRFITNRSTGKM
                     GYAMAKACAKRGAVVDLVSGPTNLEPLAGVNIHPVKSAREMYNTTLSLYDNAHIVFKT
                     AAVADYRVREVASNKIKKGEGPLTLELVRNPDILLELGRRKKQQILVGFAAETQELEQ
                     HALKKLREKNLDFILANDVGAKESGFQSDFNQGVLYRNNGDAIAIPMMDKEDFAHRII
                     DEVVKVD"
     gene            complement(10352..10558)
                     /gene="rpoZ"
                     /locus_tag="VFD89_05880"
     CDS             complement(10352..10558)
                     /gene="rpoZ"
                     /locus_tag="VFD89_05880"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZJ57756.1"
                     /translation="MIYPSLDELIDKVDSRYTLVVQVAKRARQLVDGQKTTISTDSDK
                     PVTVAIHEIKAGKVHYKKTKEGIK"
     gene            complement(10536..11171)
                     /gene="gmk"
                     /locus_tag="VFD89_05885"
     CDS             complement(10536..11171)
                     /gene="gmk"
                     /locus_tag="VFD89_05885"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZJ57757.1"
                     /translation="MGGKGLLVVISGPSGAGKGTVCREYLKSHPETLLSISMTTRRPR
                     KGEVDGVNYFFRDVKTFEELIEEGGFLEYAKVYGNYYGTPKGFVRQNLLSGRDVILEI
                     DIQGALKIKEKFDEGIFIFIVPPTMAELKKRIEGRGSETAEELLRRFKSSYRELNFIT
                     RYNYLVINDTVEEAAMKIESIVTAEKCRVDRNVDFYAKLQGGIDNDLSIFR"
     gene            complement(11186..11461)
                     /locus_tag="VFD89_05890"
     CDS             complement(11186..11461)
                     /locus_tag="VFD89_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HZJ57758.1"
                     /translation="MNIKLINIGFGNIVSANRLIAIVSPESAPIKRIIQDARDRGMLI
                     DATYGRRTRAVIITDSDHIILSAVQPETVAHRLNVKDSQAVAVSEEE"
     gene            complement(11476..12393)
                     /locus_tag="VFD89_05895"
     CDS             complement(11476..12393)
                     /locus_tag="VFD89_05895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HZJ57759.1"
                     /translation="MKGLLLSMERKRYMAQSMTGFGRAKIEGRDLEFIVEIKTLNHRY
                     LDINMRLPKVVSFLEEDIRGRIQEGLDRGRIEVYIQGSPKTGDKIEVQLNKPLVESYI
                     GCFEYLTENYNIKPDISLSALTGIQDLFQLVEKEQDEEIVKGMVLTALDEALGKVLEM
                     RNKEGYRLGEDIIMRGRFIRDMVEAIETRAPEVVDEYRAKLNNRVRELIQATEFDEAR
                     FNTEVVLFADRSNVTEEIIRLKSHLEQLGEILKQRGAIGRKLDFLVQEMNREANTIGS
                     KANDLNIINLVVDIKSEIEKIKEQVQNIE"
     gene            complement(12567..>12921)
                     /locus_tag="VFD89_05900"
     CDS             complement(12567..>12921)
                     /locus_tag="VFD89_05900"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZJ57760.1"
                     /translation="GGGGTTTASGVQVYYRDSSSDNGYAVNQKYYKNYNDSKRLKLAK
                     AMLKNTRENTDFKGRWTTPFKKDFHVLREQNLPSVLMEIGFVNNPDDVRLLNQKRTRE
                     NAAKGMYLGIVEYFK"
BASE COUNT         3224 a         3361 c         2347 g         3989 t
ORIGIN      
        1 taccaatata gaacgctaaa cccattatca tagcaaagac gaggatcata acaatagcct
       61 taatccaagg ccttttcttg ggattattat cactgattac tggttcagat atcgcacccc
      121 cgacttccct gtggttatct atactaatgg gtttcaatac ctgagttgga gcatcgctat
      181 tatagttggc aacgacaaag tcaccgttgg gttcctctgc agcccgtctc aaatccttaa
      241 tcatttctcc cgcattttga tatcgttctt cgggaagttt actgatggat ttttgaatca
      301 ccgcttgaag actcttatag atccttgggt taatctcccc ggggggtttt accatctcct
      361 ggatatgctt tatcgcaatt gatacggctg tatcaccttc aaagggcagg gtaccggtga
      421 ccatctcata gaggactata cccagagaat acagatcgga tttcgcatct atatatcctc
      481 ccctagcctg ctctggcgaa aaataatgca ccgaccctat tacacttggc ccggccaagg
      541 tgactgtggc cgaggttacg gctcgggcaa ttccaaaatc cgctacttta atagtgtagt
      601 cattcccgat caatatgttt tggggtttga tgtctctgtg gataattccg ttttcatggg
      661 cgtgttccaa ggccgccgcg atttgaaggg ctattctaat tgcctctttg gattccaaat
      721 tacccttctc cctaatgaat tccttgaggg ttttgcccca tacatattcc attactatat
      781 aatatattcc atcctcttga ccgacgtcgt atacattaac aatattatgg tgggatagac
      841 ttgctgcagc ttgggattcc cttttaaagc ggtatacaaa ctcttcgtct tctaccaatt
      901 caggtcttaa tatcttcact gcaacaaacc tgtttagaag acgacatttg gccttatata
      961 caatagccat ccctccggtg cctatctttt caaccagctc gtatctccca ccaagaattt
     1021 tacccagcaa cagcatcacc cctctttctt cttgctaata tatttgatgg ctacaacagt
     1081 gacgttgtca agccctcccc tttcaacggc ctggtctacc aagcccaggg caagctcttg
     1141 catagagtgg gcggattcca ctagatcaat gctttcttgt aagtctacat ggaggattaa
     1201 tccatcagta cacaataaaa taatgtcatt ctccttcaag gctattttat gacagtctat
     1261 atcaatgaca ttttcggtac ccaatgctct ggttattatg tttttttggg ggtgtttgtc
     1321 tacctcgtcc ggtgtaatag taccattatc caatagttcc tgtaccaggg aatgatcctt
     1381 ggttaattgt ttaacgcttc cccccctgat caaatacccc cggctatcac caacatggcc
     1441 taggtagaga atttcgtggg aaaaataggc cgttatcaag gtagtaccca tcccgttata
     1501 ggcctcattc gccagggaga catcaaatat cctgccgttg gaaaccccaa tagcctcttc
     1561 gataagtccc gcaggatctt gactgtatcc gtcccggtca atcctatggg ttagatattc
     1621 tattgcagtt tctacagcca tacggctggc cagttcccct gcattgtgtc cccccatgcc
     1681 atcggcgatc atagccaagt cttggatttt atcggcgctg ggaatatagt agtaatcctc
     1741 attggatttt ctaaccatgc ctttatggct atatccacca tactctaata tcatttaccc
     1801 cacctcacct tgttgctcct ttgaccgatg tgcctgccat cgtcaaacta tatattttac
     1861 gaaaaagatt ccccatcaaa gtcatttaaa tacccactct ttaattgtcc gcatgcccca
     1921 cctatatcct gtcctaactc tcttcgtctt gtaacctgga tacccgcgga cctaaggttt
     1981 ttttcaaaga gttgtatcct ggcctcaggg cttcgattat acggtgcacc ttcaatagta
     2041 ttgatgggaa taacattcac atgacatgga aaatccccaa gcaacttgcc taattccatt
     2101 gcttcatcgc tcccgtcatt aataccgtcc ataagggaat actcaaagct tacccttctc
     2161 ccggttctct caaaatagta tttcgctgct tcgactatct ccagtatccc ataagctgaa
     2221 gctgccggca taatcctacg ccttaactta tcgttgggag cgtggaggga tatagccaag
     2281 gtaatgggca tatcctcgtc agccaatctt cttatccccg gtaccagtcc gcaggtggaa
     2341 agggttatat tcctatagcc tatattcaag gtctccggat cgtgaatcag acgcaggaac
     2401 tttactacat tgtcatagtt atccaaaggc tcgccgctac ccataaggac aatatttcga
     2461 atgttgcgtt ctccggtccc gacagtgccc atatccgtat tcgccataag gacctgggct
     2521 accatttcac ccacctctaa attacgtatc agaccctgcc tggtagattc gcaaaaactg
     2581 catcccatcc tgcatccaac ttgggtggac aggcatagag tatttccgta gtcatacccc
     2641 attacaacat cttctattat attatcatca tggagtaaac acaaatattt tattgtatcg
     2701 cctttatcgg agacgatttt ttccatttga tttactcttc ctatcacata tctttcccta
     2761 agtttcgtcc tgacggtcat agggagattg gtcatatcat caaagtcttc aattccctta
     2821 tacagccaat ttacaacctg ctttcccctg aacccctgct ccccgatttg ttttaatatc
     2881 tctacaattt ctgcaggtga tttgtccagt atatccattc tcgtacccac tatagtgcgg
     2941 acctcctcat ttttgcaata aaaaacccat ctactccatg gcgatggggg aacaattgga
     3001 tcattccctc acctattgta gcatctttaa ggcccatagg cagctcccgg ttgaaatctt
     3061 ccaatacaaa ttcaggatat ttctctacaa acccttcgat agccttatga ttttcccacg
     3121 ggtttattgt gcatgtactg tagaccaaag tccccccggg tttaacatac cttctgcaag
     3181 tactcaatat ttcccgttgg agcctataaa gggattccat atccctttct tctattctca
     3241 gcctaatatc cggttttttg tggaggacgc cccaacctga acagggagca tctattaaga
     3301 ccctgtccat actttgttcc atcttgggat tgaattcgcc ggcatcctgc acacccgttt
     3361 caatgttggt aatacccatt ctctctatgc ttttgtctat tagtcctact ctatgctcat
     3421 ggatgtccct ggcgtacacc ttgcctgtca tacccataag ctccgctata tatatagcct
     3481 ttccgccggg agcgctacac gcatccaaaa tgcattcccc gggcttcggt gtcagaatct
     3541 ggcttacaag catggaactc tcaccctgta cagtaaacat ccccttttta tacagggggt
     3601 ttgcttgtat gttcccaatt ccctccaccc tcagagcatt attaaaatat gggctctccg
     3661 cagcttttat cccctgttgt ttcaattcat ttataagtcc atccctactg atcctagcct
     3721 tatttatcct aagggtgata taattagccc ctatagtaga ttccattata gctctagcca
     3781 cgtctacccc atattccccg gcccataaat ccaccaacca caacgggaac ccgtatagcg
     3841 atgccaagga ctttgtgtcc gtgggtatcg gctctgatgc catcaattct tttttatggc
     3901 gagaaatatt ccttaagaca ccgttgacaa aacccttcaa ggatccaaac ccaaatccga
     3961 cacatagctt taccgattca ttacaggcgg cggaatccgg caccctgtca agaaacagta
     4021 tctgataaca gcctagcctg aggatatttt ctacccaagg attaaccccc ctcatcttgg
     4081 caaatttcct aagaacatta tcgataaagg cttgattttc cagggtcccg tataccagtc
     4141 tggtaacaaa ggaggcatcc cgatcctgaa aactaatcct atttaggttc tccttgagac
     4201 ttatattggc gtattttccc tcttcattta catcttttaa aataatcaaa gcagtttttc
     4261 gaaccctatc cataactcct ccatacccat tcaaaaaagg gcctattagc ccttattttg
     4321 ttatctcctt cttctgtccc ttatcaaaag caaccttagc aattgggcaa cagccgatgc
     4381 ggcagctgcc acgtaggtaa gggctgcagc gtttaatacc ttctttacat ttccctcttc
     4441 ttctctggta acaaaaccct ggatgctgag gagctcaaca gctcggctac ttgcgttgaa
     4501 ttctactgga agggtgatca attggaaggc tacaactcca agaaagagat atattcctat
     4561 atccagcata accgtagccc ccataacaag gcctaaaaat atgaagagaa aggccccatg
     4621 ggagccaaaa ttggctacgg gcacaatcgc attccgaata ctaaggggcg catatccatt
     4681 ggcatgttgt aaggcatggc ccgcttcatg ggcagccacc ccaattgatg caatcgatct
     4741 accgttatac accgcatcgg atagcctaag aacttttttc ctgggatcgt aatggtccgt
     4801 taaatgtcca cctgtcctat ttacgggtat atccgccaat cccatagtgt ctagaatttt
     4861 tcttgctacc tctgccccag tatatccgcc ataatttgct actctgataa acctattaaa
     4921 agtgctttgc accttgtatt gcgcataaaa ggatagaata agggcgggca acaaaattag
     4981 catagtagaa tcgtaaaaaa gaaaaggcat tatatcccac ctttcgaaat tagacttctt
     5041 gataccttaa aacatcggaa gcaattgcat tggcaattgc aacacacagg tttcaacgag
     5101 gcgggtgatt tatacccacc gcctgtttta ctataatcgg aactcgccgc ttatagaagc
     5161 gggagacatc ttgccgactc gttatatgat acaaattctt ctaccccttg aatcaactga
     5221 tagcccggtt ctaatcctcg gcatccagaa gggttttgac cttaatcggg ttcccccgta
     5281 aaaactccac atcctccata acccgtttac cgggcgcctg tactttgaac aacctgatca
     5341 atccattgga tccggatatc acaagcccgt tctcctcatc agcatggaca acctgtcccg
     5401 gaacaatatc ctttaattca aagtattccc cttggcccgc ttcccatact ttcaggcgta
     5461 taccgtcaat atgggtataa gcccccggcc acggggttaa ggccctaata tggttcctta
     5521 ttctgtctat atccatatcc cattgaatct tgccgaaggc tttatctatc ttttgacaat
     5581 aggtggcttc ctcgtgtacc tggggtattc ctacgggttt tccctcagca aatagtctga
     5641 ggctctccct aagggcctgg ccgcctaaat ctgctagccg ttcatgaaga tccccactat
     5701 tttcacctgg gtgtatgggt atttcccttt gtaaaatgat atcccccgta tccataccta
     5761 catccatata cattatggtt attcccgttt gggtttcccc atccataaca gcttgctgta
     5821 taggcgaggc tccccgatac tttggtaaaa gggatgcatg tacattaata cagccaaggg
     5881 aaggtatatc caaaattgtt ttgggtagga tctgcccata ggcggcggtt attataagat
     5941 ccggagccaa gtcagtaatt tggactataa atccttcatc ccgcgccctt gacggttgaa
     6001 ataccttaat ccctttttcc atggcccatg ccttcacagg tgagggcagt aagcgttttc
     6061 ctcggcccct tggtctgtca ggctgggtaa caaccccaat cagttcgcat ccttcagata
     6121 taatagcatt taacgaagga attgaaaatt ccggtgtacc cataaaaatg attttcaatt
     6181 taactcatcc cctattttct gtttcctggt cgaccctttt tagcgtaccc ggctctatcc
     6241 tatctatata caaaatccca tctaggtggt caatttcgtg gcaaagagcc ctagccaaaa
     6301 gagcctcacc ctccagttct attttttcac cgtcgcggtt tagccccacg attttgacct
     6361 tgtcaggtcg ttttactata ccggtgaggc cgggaatgct caggcaacct tccatatcag
     6421 cctgttcacc ttgcctctca acgatctcag gatttattaa ctctataatt ccatccccta
     6481 tatctataac ggcaacacgc cttaaaatac ccacctgggg cgcagccaac cccacgcctt
     6541 cggcattata catagtttcc gccatatcgt ctaatagaag caatattctt ttatcaattt
     6601 tctccaccgg tcttgccttt tttctcaata catcgtctct ttcgtaatgt ttaatctccc
     6661 ttagggccat attatcacct ctttttaaat caacaaatta aaacatcggg agcgggggac
     6721 atcttgccga cccgcaatag tctgggatgt aggatatatg catccatctg cttatagtaa
     6781 actagtggga tagaaatcaa ccactatagt accagctgca ttaaaaaatt ccctttggca
     6841 atcgtccact atttcatgat atctttctat aaaaattcgt tcattacgaa tacgggccag
     6901 aacatgccac ctatatctat tctttatcct ctcaaggggt gcaggatagg cacccagctc
     6961 tatagtcccc gattttatga tgggatcttc tgccccttta tattccatcc atttttttat
     7021 gtccaaagcc gttttaatga cgttgtcctc aatctctcca gttattaata ccctgactat
     7081 atgagaaaaa ggcggatatt tgaattggtt ccttatcttt atctcctcat tataaaaccc
     7141 cagatagtcg tgtttggaag aatattggat agcatagtga tccggatgat aggtctgtat
     7201 aataacctta ccttccctac taccccttcc agctcgtcct gccacctggg ttatgagctg
     7261 gaaggtcttc tcgctactcc gataatcagg tagatttaag gaggtgtcgg ctgtaactac
     7321 gcctaccaag gtcacattgg gaaaatccag tcccttggca accatctggg ttcccaataa
     7381 gatatcatat tctctatgtc taaaggcatc caaaatcctt tgatgggccc cttttgtaga
     7441 tgtagtgtcc atatccattc gaattatcct agctgaggat aagagcctgc ctatttcctg
     7501 ttccaccttc tgggtcccta cgccaaaatg tttaatatat gtgctcttac actccgggca
     7561 aatcctagga tagggtttgg tgtttccgca ataatggcat tttaatacat tgctcttgga
     7621 atggtatgtc atggaaatat cgcaattatt gcatttaacc acatagccgc aggacctaca
     7681 ggatacaaac tgggcgtatc cccttctgtt tagcagaata atagcctgtt ctcccctgga
     7741 caaggctcct tggagttcat cgtatagctt ttggctaaag atactgcggt tgcctctttt
     7801 gatttcaaga cgcatatcta ttatctcaac cccgggcaaa ggtctgtagt taattcgatg
     7861 atccaacgtc accctagtat aaagcccctt ctcgctatta taaaaatcat ccaaagaagg
     7921 agtagcactg cccaacacaa gaacagcccc ctgttccttg gacctttggg ctgctaatcc
     7981 tatggtatga tatcttggcc tggattcgga tttatagcta tcgtcatggg cctcatcgat
     8041 aactattaaa cctaggtcct gcaaaggggc aaatatagct gatctagccc ctattactat
     8101 atttacctgg cggttgtata ttcttctcca ttcatcaaac ctttcacctg tggaaagcct
     8161 gctgtgaagg atcgccacat ctgcaccaaa ccgtttcttg aaatgaccaa cagtttgtgg
     8221 ggtcaatgaa atttcgggta ccagtataat ggcccttttt ctattcttga gagttttttc
     8281 taccagatgc atatatactt ctgtcttccc gctccccgta actccgtgta ggagatagat
     8341 cccttgggac atggaaatcc cttcgccaat ggtttcaata gctatcgcct gctccttagt
     8401 aggctccagt tccgtttcca atacatcgat gggctgatcc caaggatttc tgtagacctc
     8461 ttgatcttct agaaaaatcc atccccgctt ttcaaggttt ttgaagctgg ataggggggc
     8521 accccctgcc atatccgcca attcgggcaa tgagatacca tctccttcat ttagaatcct
     8581 gaggatttct gccatatatc tggagcgact ttccacctgg gctattttct catcgatatt
     8641 agtctccact ttaaggaaga ccattttttc tactttacca tccatatttc tacgaagccc
     8701 cggagggata aagcatctta tggcctcaat ggtgagacag tgatattcct ttttcatcca
     8761 ccaaactagg ggaactaatt caggaaggag cactggataa ggatcccgta tttcctttat
     8821 gggctttact ctattatggg gaataaatat ctccttgttc agccccataa tataggcatc
     8881 tatattcctg tttcccgccc caaagggcac tagcaccctc attcctattt ggatcccggc
     8941 cttgagatct tccgggattt catagtggta tatcttgtct atggccggat gggcctggtc
     9001 tactataacc ccagcataaa gcttatccga catggcctac ccaacctccc tgcttattat
     9061 ataagttaac tttttgcgct agttaaatat atcgcatagt aagataataa accagcgcaa
     9121 taggttatcc ccgtataagg gtcttgagca actaatccac ctttactacc tcatcaataa
     9181 tccgatgtgc gaaatcctct ttatccatca tgggtatagc tattgcatcc ccattgttac
     9241 ggtataggac accctgatta aaatcgcttt ggaagcccga ctctttggcg cctacatcgt
     9301 tggccagaat aaaatccaga ttcttttccc tcagcttttt tagggcatgc tgctccaatt
     9361 cctgagtttc agcagcaaag ccgacaagga tctgttgttt ttttcttctg cccagctcca
     9421 acaaaatatc tggattgcgg accagttcta aggttaaagg accctccccc ttttttatct
     9481 tattggatgc gacttctcgc accctgtaat cggctacagc cgctgtttta aatactatat
     9541 gagcattatc gtagaggcta agagttgtat tatacatctc cctcgccgat tttacaggat
     9601 gaatattaac ccctgccaga ggctccagat tcgtaggccc tgataccagg tctaccacag
     9661 cacccctttt agcgcatgcc tttgccatgg cataccccat ctttcccgta gatctattgg
     9721 taatgaatct gactgggtct atggattcga tagtgggccc ggcggtgacc aagaccctct
     9781 tacctataag atcccggttt tccaccagat tatcttccag aacctttagg atatcgtcta
     9841 cgtctgccag ttttccttcg ccccaatccc cacaggcaag ccttccactt ccggggggga
     9901 taaaggtata gcccaatgcc ttgagttttt ttatgttatc ttgaacgacc cctttttgat
     9961 acattacggt gttcatggca ggcgcaaaaa tggtctttgc atcggtggcc aagatcgttg
    10021 ttgacaatag atcgtctgca ataccaccag cggctttacc tataacattg gcagtcgcgg
    10081 gggcgataat gaagacatcc gcccatttgg ccagagcaat atgctctata tcatagcgat
    10141 ccgttgtatg aaaggtatcg tggtatactt gatttccgga taaactctcc agggtcaagg
    10201 gatggataaa ctttgtggcg ttttccgtca taactacctt tacccctgcc ccaagtttta
    10261 tcattgcgct ggtcaggtca gccgccttat aagctgctat acccccggat attcctatca
    10321 aaatattttt tcctgcaaaa gcgctgttca tttatttaat accctctttt gtttttttgt
    10381 aatgaacctt gcctgccttt atctcgtgta tggcaacggt aaccggcttg tcggaatcag
    10441 ttgatatcgt agtcttttgt ccatccacca gctgtctggc ccttttagca acctgaacca
    10501 ctagggtata cctgctgtcc actttatcta tcaactcatc taaagatgga taaatcatta
    10561 tctatacctc cttgtaactt agcataaaaa tctacattcc tatctaccct gcatttctca
    10621 gcagtgacaa ttgattctat tttcatagcg gcttcttcaa ccgtatcgtt gatcactaga
    10681 tagttatagc gggttatgaa gttaagttcc ctgtaggaac ttttaaatct tctaaggagc
    10741 tcttccgccg tttcgcttcc ccgcccttcg atccttttct ttaattctgc catagtaggc
    10801 ggtacaatga agataaatat cccctcatcg aatttttcct ttatttttag ggcgccctgg
    10861 atatcaattt ccagtattac atcccttcct gataacaggt tctgccttac aaaacccttg
    10921 ggtgtaccat aatagttgcc atagaccttt gcgtattcca aaaatccccc ttcttcaatc
    10981 agttcctcga aggtttttac atccctaaag aaataattga caccatcgac ttcccctttt
    11041 cggggtcgcc tagtggtcat ggagatggac aaaagggttt ccgggtgact ctttaggtat
    11101 tcccgacata ctgtgccttt accggcccct gatggtccgg atatcacaac cagcagtcct
    11161 tttcccccca tacatatatc ccccttcact cttcttctga aacagctacg gcttgactgt
    11221 cctttacatt tagcctatgg gcaacagtct caggttgcac agctgataat attatatgat
    11281 cgctatcagt gatgattact gcgcgggttc ttctgccata ggttgcgtct atcaacatgc
    11341 ccctatccct tgcgtcctgt ataatccgtt ttataggcgc tgattcgggg ctaacaatgg
    11401 caattagcct gttagccgaa acgatgttgc caaaacctat gttaatcaat ttaatgttca
    11461 tattagtacc gcctctcatt caatattttg tacttgttcc ttgatttttt caatctcgct
    11521 ctttatgtcc actacaaggt taattatatt taaatcgttg gcctttgaac ctattgtgtt
    11581 tgcctcccta ttcatctcct ggactaaaaa atccaatttt ctaccgatgg cgcccctctg
    11641 ctttaatatt tcccctaatt gttctaggtg actcttgagt cttataatct cctcggtgac
    11701 gttggatcta tcggcaaaga ggacgacttc cgtgttaaat ctagcctcgt caaattcagt
    11761 tgcctggata agttccctaa ccctattatt taatttagcc ctatactcat caacaacctc
    11821 cggggctctg gtttctatgg cctctaccat atcccttatg aaacgcccac gcattattat
    11881 gtcttccccc agcctatacc cctctttatt tcgcatttca aggactttac ctaaggcttc
    11941 atccaaagcc gtgagaacca tccccttaac tatctcttca tcctgttctt tttcaaccag
    12001 ttgaaagaga tcctgaattc cagtcaaagc tgacaaggag atatccggtt ttatattata
    12061 gttttcggtg aggtattcaa aacagcctat gtaggattca accaagggct tgttcagctg
    12121 cacctcgatc ttatcccccg tctttgggga tccctgaatg tatacttcaa tcctgcccct
    12181 atcgagcccc tcttgaattc tccctcttat atcctcttct aagaagctta caaccttcgg
    12241 cagtcgcata ttaatatcta aatatctgtg atttagagtt tttatttcta caataaactc
    12301 caagtctcga ccctcaatct tggcccgtcc aaacccggtc atactctgcg ccatgtacct
    12361 cttcctctcc atgctcagca ataatccctt catatcctac ttaagggagc cttttccttg
    12421 tattatagca caaaggccgg gtatttaaaa cttatctttt tttggtggat acagctattt
    12481 caattctcgc cccacataaa agataaaaag attgggggat aagtaccccc caacctcctt
    12541 tggatattac atcatcacgg aatttcttat ttaaagtatt ccactatacc caagtacatg
    12601 ccctttgcgg cattttcacg ggtccgtttt tgatttaaca gtcttacatc gtcgggatta
    12661 ttaacaaacc caatctccat caagacagac ggcagatttt gctccctaag aacatgaaaa
    12721 tccttcttaa agggggttgt ccatcttccc ttgaaatccg tattctcccg ggtattctta
    12781 agcattgcct tagccagctt taagcgctta ctgtcgttat agtttttgta gtatttctgg
    12841 ttgaccgcat atccgttgtc ggagctgctg tccctataat atacctgcac tccggaagct
    12901 gtggttgtac cgccgcctcc c
//
LOCUS       DASRNX010000144        17203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_400307,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000144 DASRNX010000000
VERSION     DASRNX010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17203
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400307"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..956)
                     /gene="hisIE"
                     /locus_tag="VFD89_05905"
     CDS             complement(282..956)
                     /gene="hisIE"
                     /locus_tag="VFD89_05905"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HZJ57761.1"
                     /translation="MCDVDIKFDDQGLIPAIIQDIETDEVLMMAYMNKESLDKTLMTG
                     KTFFWSRSRKRLWQKGEESGNIQQVKEIYYDCDKDTLLVKVIQKGGACHTGHRSCFYR
                     KLEHVNFIIPGKEDKDFGASMVHGKATILQSLYDTIMDRKKNPREGSYTSYLFEKGID
                     KILKKVGEESSEVIIGAKNGDEDEVVYETADLFYHLIVMLVDQGITLDRIYEELGKRR
                     RSSQDR"
     gene            complement(961..1725)
                     /gene="hisF"
                     /locus_tag="VFD89_05910"
     CDS             complement(961..1725)
                     /gene="hisF"
                     /locus_tag="VFD89_05910"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZJ57762.1"
                     /translation="MLTRIIPCLDIRDGRVVKGVKFADLKDAGDPIEIAKAYAGAGAD
                     ELFLLNVAATAENRAITLNIIREITRKTDIPLTVGGGISKLENFHDFFSAGAHKVSIS
                     SAALKTPELITEVADHFGSKHIVVAIDVIKGPQGNWSVCVDGGKVNTGMDAVKWAMRA
                     EELGAGEILLTSMDRDGTKEGYDIELLRTVSGSIDIPVIASGGAGRMAHFLDAIIKGG
                     ACAVLAASLFHYKELEIGTLKEYLWQNGVKTSGKEG"
     gene            complement(1729..2448)
                     /gene="hisA"
                     /locus_tag="VFD89_05915"
     CDS             complement(1729..2448)
                     /gene="hisA"
                     /locus_tag="VFD89_05915"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HZJ57763.1"
                     /translation="MNIYPAIDIRDGRCVMLMQGDADKETVYSNNPVEVARRWQDMGA
                     KFLHIVDLDGAFQGESRNIEVIMEVIESVSISLQIGGGFRTLKKIELFLEDPKVARVI
                     LGTSAVTKPQLLEKAIGIYGDRIAVGIDAKDGQVAIRGWVEKTETDSIEFGKRLYDMG
                     VDTVIYTDIEKDGMLKGPNIEATKAMIDETGLKIIASGGVTSLEDIKLIKGIGAEGVI
                     IGKALYSGAIALGDALAYEGV"
     gene            complement(2466..3065)
                     /gene="hisB"
                     /locus_tag="VFD89_05920"
     CDS             complement(2466..3065)
                     /gene="hisB"
                     /locus_tag="VFD89_05920"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007957660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZJ57764.1"
                     /translation="MARTGTVKRESFETKVSATVEIDGSGRNNIKTGIGFFDHMLDLL
                     SSHGRFDIDISCIGDLQVDGHHSVEDIGIVLGRAFKKALGQRMGINRYGNMTLPMDET
                     LAEVSLDISGRPYLHLEMPALTQFVGDFDTQLLEEFLRSFSNHLGLTLHVRVAYGRNT
                     HHIIEGIFKALGRALRQAVAVDSTLADRDVIPSTKGILD"
     gene            complement(3284..4351)
                     /gene="hisC"
                     /locus_tag="VFD89_05925"
     CDS             complement(3284..4351)
                     /gene="hisC"
                     /locus_tag="VFD89_05925"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZJ57765.1"
                     /translation="MKINYLRRDLMNLKAYDAEEKSCIYKMDANESPWDLPDSFREIL
                     ARDLMDGKDFNRYPDSKSTNLRRAIGEHWGVSSDQVIVGAGSDELIQILITGFAGKGE
                     GVAYPTPSFGMYQIFTLIAGATPMPIELGEGYEYDMEAFTSAISKYNPKVLFICNPNN
                     PTGNLMDPDQLKKLINAFPGVVVIDEAYGEFAKASMIEYAIQVPNLVVLRTFSKAFGL
                     AGLRVGYCITSPELASQINRVMPPYNLNTFSQDAAVLALANREQMDIRVKKIIEQRQI
                     MYNALGKIDGLRIYPSQANFLLIKVPRGHLTWQQLLDRGILVRDFSGEPYLRDCLRVT
                     IACEEANRAFISSMYEIFSEG"
     gene            complement(4348..5649)
                     /gene="hisD"
                     /locus_tag="VFD89_05930"
     CDS             complement(4348..5649)
                     /gene="hisD"
                     /locus_tag="VFD89_05930"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZJ57766.1"
                     /translation="MILQIDGRGTESSEIIALLKRPSQLDFPEEGNVVKEILSEIKSG
                     GSAALFKYIEQFDGVNYKDPQKMLVSGEEIKTAYTVVSKGYIDIIDRAMDRIARYHKK
                     QKQNSWISFEDDSGVVLGQKIEPMEWVGMYVPGGRAAYPSSVLMNAIPAEIAGVDNII
                     MATPPGPGGVVNPYTLVAADMAGIGDIYKMGGAQSIAAMAFGIEPVPKVDKITGPGNI
                     YVTLAKKEVYGYVDIDMIAGPSEVVVIADSSANREYVAADLLSQAEHDPLATSVLITD
                     NIDLYNNIGCEIERQAKTLPKLDIVEESLSNFGAIILVDNIELAIELSNYIAPEHLEL
                     AIENPFEKLGKVKNAGSIFLGHYSPEPLGDYMAGPNHILPTSGSARYFSPLSVDDFIK
                     KSSILSFSKEALEDLYKDVADFARIEGLEAHARSVEARFRQ"
     gene            complement(5651..6283)
                     /gene="hisG"
                     /locus_tag="VFD89_05935"
     CDS             complement(5651..6283)
                     /gene="hisG"
                     /locus_tag="VFD89_05935"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZJ57767.1"
                     /translation="MITIALPKGRLGERTIELLQRAIGNLNDLDISSRKLIIYSNNEK
                     IRFLLVKPSDVPTYVEYGVADIGIAGKDTLLEEERPLYEVLDLKFGKCKMAVAGFPEK
                     KDAWITKSHTRVATKYPNIARNYFSKAGETIEVIKLNGSVELGPLVGLSDVIVDIVES
                     GRTLKENGLVVLEDICDISARLVVNRISFKTKNEPIKNLIESIERVLEEG"
     gene            complement(6280..7482)
                     /gene="hisZ"
                     /locus_tag="VFD89_05940"
     CDS             complement(6280..7482)
                     /gene="hisZ"
                     /locus_tag="VFD89_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HZJ57768.1"
                     /translation="MKKWKQHTPQGVQDYLPEECYNKGKIEESIRQMFFSRGYDEIES
                     PVFEFYDVFTGDKTYIEQEKMYKIVEAGGRIQVLRPDITMPIARMVGTKLRDAMLPIR
                     LSYLGNVYRYEGLQTGNQREVAQAGIELFGVDGPEADAEVISTAIEVFLSLGLEDFQI
                     DIGQVDFFKGLTEDAGLTLEVREELRFLIEQKNLLALETLLGELPIDGALKNIFMQLP
                     SMYGDIDVLKQGLGYTKNPRCLKAIENIHEVYEILDDFGLSKYITFDLGMVHSLNYYT
                     GIIFRGFTKNLGFPICGGGRYDNLLREFGYDLPATGFAMGLKRLLVALDRQKKLTPLP
                     GIDILVVPDRERGREGYNLIKRLREEGNRVEVYLKADKGQDPFEYARARGILKVIDMK
                     DYDREGVL"
     gene            7814..8617
                     /locus_tag="VFD89_05945"
     CDS             7814..8617
                     /locus_tag="VFD89_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HZJ57769.1"
                     /translation="MDYHIHTRYSSDGEMTIEEACKRAIKIGLKEIAITDHIDIDWPN
                     PDISFDIANIDEYIQEIHDNKERFKSQLIVKVGIEIGLQPHVLEESAKIIRSHPFDFV
                     IGSIHIIKGMDPYMGDYFYGKTKSESYEIYYRETIDLIKEFDDFDVLGHLGYIKRYFP
                     QPYSKEDEYLHLDLVDEILEILIQKNKGIEVNTSGYKHSSACPMPSLNTIARYKQLGG
                     SIITVGSDAHSVDSIGYGIGRGTNAIKNLGFDSVTSFTSRKPEFRSIGD"
     gene            complement(8675..10696)
                     /locus_tag="VFD89_05950"
     CDS             complement(8675..10696)
                     /locus_tag="VFD89_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZJ57770.1"
                     /translation="MNCEFSDLVDIDRFQELFDSIYKATGIAAGIADANGIVLTRTGW
                     TDVCTRFHRGDPRIANRCVESTKSAPELAAKAIAKGEGYVYYKCENGIMHAITPIYIK
                     DKCVAVIYKSQFFLSDPDLEYFKAQARTLGLPEGEYIEAIKKVPVYSKEEMDRFMEFA
                     SRIAKLFAQIGYDQITLLEEQTKSEHRYQELMAKHQELMAIYEEIGDMEEELHYRKHK
                     IEHQEGALKVSQERIRYLAYYDAVTGLPNRLNVSDRITHWIASAKRENKKGALFFLDI
                     DNFKVINDTFGHGFGDKVLKRFGTGLAKISEGKGLVGRFGGDEFIMVRFGIEDRQDVE
                     NTISSILGIFDAPWVIDGFEVYPTVSVGITMFPDDGDTTEVLLKNADIAMYEAKKYGK
                     NTYEFFEKSMNDRVIHRMEMDRDLRNALDRDEFHLVYQPQVNSKTGRVEAVEALIRWN
                     HPKHGLIPPAYFIPFAEDTGLIIPIGEWVLRTACKQNKKWQDKGYNPIRVSVNISPLQ
                     LRRWNFISLVRDILEDVELDPSYLELEITENNIMESTENNIEALQQLYQMGVRIALDD
                     FGTGYSSLNYLHRLPINNLKIDRSFVEDITNDSDKRYIAQVIINLAHRMNLMVTAEGV
                     ETEEQLRMLVNQRCDMIQGFLFSRPLPSEDIEEILRRGRLDIAEYVQMV"
     gene            complement(11020..11469)
                     /locus_tag="VFD89_05955"
     CDS             complement(11020..11469)
                     /locus_tag="VFD89_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HZJ57771.1"
                     /translation="MQENYHRHQISDFLTKHLKTIRSTRNHILKEFYEEHTGERDAFK
                     GMMDQYLDFLKGFIDANPEVSVKQRWPLILIGSVAEVQDLDYSEIERLKIVPPFYGDG
                     GGGLDCASCLSPVGYALLFKGVGDRVTVDTPLGNCRFMIKSVELPPV"
     gene            complement(11655..12809)
                     /locus_tag="VFD89_05960"
     CDS             complement(11655..12809)
                     /locus_tag="VFD89_05960"
                     /inference="COORDINATES: protein motif:HMM:NF019097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HZJ57772.1"
                     /translation="MERIRINKKSNLSYNLVVILLIIGIALLSYRLLSNHEAFLGKGD
                     GFPDESTNIKEKSDAYISGNKGDFFSDIWIRLKEMNFTDPAFILSSHMPYLSAAYAQN
                     EPIYAENGAESVVAAVATPEQETSRQANRPTDLDLERYEEAVGEVSEEIQIQVKKISD
                     DLPPIALKGDGPQILIYHTHSREAYRQDPNNPYKDIAVFRSTDFSQTVVGVGEELTNQ
                     LQNRGIQVLHDTTDHEVYPWKSGLYPRSLETVKKRQKEYKSLKIYLDIHRNYIKGLKD
                     PDKEVTIIDGERVAKFFVLIGTAQGVSGGFSQKPEWRENYQFGLKITNKANEKYPGLA
                     NKVKLQTGRYNQHVSNRAIIIEVGNHLTTFAEAKRSAKYIAEILSEIIEK"
     gene            complement(13362..14789)
                     /locus_tag="VFD89_05965"
     CDS             complement(13362..14789)
                     /locus_tag="VFD89_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HZJ57773.1"
                     /translation="MKSFKILLCTVLLTLLLTGVTIPALAYTGGSSTVYTMYINDEQV
                     GIVKFPARALSIYDDIERRQRGEYQEEVFIDSKIYFKETNVGADEITDDDELANSIEE
                     LIDIRVDAYSIDIDGTRVCYVKTMEDAQEVIYNIKAPYIEKIKEKEDSQLEDVAFKQD
                     IDFQEGITSVKNILSPEKAAEAILHGGQEIKEYRVQEGDSLWSIAQQNHVSVSDLELA
                     NPDLDGDIIQPEDIIMIGQQKKLLTTVTKEKVRYSKEIPFETETKEDNGLEKGKTKVV
                     QQGENGKKDIVALVTREDGQEVSRDIIEEKVEKEPVKHIIAKGTKEKPKPAPRPKPVR
                     RPATNSKSTSKPKSSGNTTTDRGTVSGSASSVVDFAMKYKGYKYVFGTSGPNTFDCSG
                     FTKYVYSQFGVSLPHSSKSQRSVGSGVSKENLKPGDILCFSGHVGLYIGGNQFIHASN
                     KKDGVKISNLSSYNKRLITARRIFK"
     gene            complement(14929..15759)
                     /locus_tag="VFD89_05970"
     CDS             complement(14929..15759)
                     /locus_tag="VFD89_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HZJ57774.1"
                     /translation="MKPLVKTIRNENNDFQRIEVVKRNREKRNRYREFFVEGVKPINF
                     AIENNWEIRSFVYSGEAPLSDWAKGVLRNSTAKTHFELPLRLMDKLSDKDEELSELIA
                     IVSIPGDDIARIRVGDDTLLVVIDRPSGYGNLGTIIRSCEALKVDGIVVTGHAVDIYD
                     SRTIRASMGTLFAVPIIRLQSHRQLLPWFNDIRKIHRDFKIVGSSAGGDALIMDADLA
                     TRPMALIIGNETLGLSYNYKQLCDVLVRIPMHGHITSLNIACAASIMLYELERQRNFN
                     "
     gene            complement(15924..16796)
                     /locus_tag="VFD89_05975"
     CDS             complement(15924..16796)
                     /locus_tag="VFD89_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZJ57775.1"
                     /translation="MKLGVSSYSLINLVRSGQIKQIDVISLAKDLGCDLIEFVPFILE
                     ENETNEDFAVRAKAECARVGIEMGSYTVGADFINGSDGDLDAEIQRVKGEVDIARLLG
                     SKTMRHDATVGFSKEHTGPRDFDAALPRLIEGCRAVTEYAQEFGIQTMIENHGFFCQD
                     SDRVEKLINGVDHPNFGLLLDMGNFVCVDESPETAMGRLMPYAFHVHAKDFHIKSGMH
                     PDPGQGWFRSRGGNYLRGAIIGHGDVAIAQCLRIAKDSGYKGALSIEFEGMEDPKAGV
                     GIGLENLRKYLALI"
BASE COUNT         4527 a         4285 c         3134 g         5257 t
ORIGIN      
        1 caacagagaa gtacatagaa ttgatttatc taagtttcga aataatccta tattcactat
       61 accacatacc cgagtccatt gccataatgg atttttattt gaaaggccga attcattcta
      121 aaggcttata ggtatagatc cctgctatta ttttcctcgg ggagcaagga gaacattaaa
      181 atttaaatgg tatattcaaa accaaaagga ccgattagtg tcatagaaca ctttcgatcc
      241 ctactttact atatatcctg gtcttattcc gctttccggt tttacctatc ttgggaactt
      301 ctgcgtcttt tccccaattc ttcatatatt ctgtctaagg ttataccctg atctaccagc
      361 ataactatga gatgataaaa tagatcggct gtctcataga ccacctcatc ctcgtcgcca
      421 tttttagcgc ctattattac ctccgatgat tcctcgccaa cttttttcaa tatcttgtct
      481 atcccctttt caaataggta gctggtatag gaaccttccc tagggttttt cttacgatcc
      541 attatcgtat catataggct ctgtaatatt gttgccttac catggaccat ggatgcccca
      601 aaatccttat cctctttgcc cgggattatg aaatttacat gttccagctt tctataaaaa
      661 caggacctat ggcctgtgtg gcaggctccg cccttttgta taaccttcac caaaagggta
      721 tccttatcac agtcataata tatttccttc acttgttgta tatttcccga ttcttcgcct
      781 ttttgccaaa gtctttttct ggacctgctc caaaaaaacg tctttccggt catcagagtt
      841 ttatctagag attccttgtt catataggcc atcatcaata cctcgtcggt ctctatatcc
      901 tgtattatag cggggattaa accctgatca tcaaatttta tatccacatc acacatagta
      961 ttaaccttcc ttccccgagg tttttactcc attttgccat aggtattcct ttaatgttcc
     1021 tatttccaat tccttatagt gaaaaagaga tgcagctaaa acagcgcatg ctcctccctt
     1081 gattatggca tctaagaaat gtgccattct acccgcacct cctgatgcaa taaccggaat
     1141 atctatgctc cccgaaaccg tccttaatag ctctatatca taaccttcct tggttccgtc
     1201 cctatccata ctagtcagca aaatttcccc agcccccagc tcctcggctc tcatcgccca
     1261 cttaaccgca tccatgcctg tattgacctt cccgccgtct acacacaccg accagttccc
     1321 ctgaggtccc tttataacat caatagcgac tacaatatgt ttactgccaa aatgatcggc
     1381 tacctcggta atcaattccg gcgttttaag agcagcagag cttatggata ccttatgggc
     1441 tcctgcacta aaaaaatcat gaaaattctc cagtttacta attcccccgc ccaccgttaa
     1501 ggggatatcc gtctttctag tgatttccct gataatgttt aaggtaattg ccctattttc
     1561 ggctgtagca gcaacattca ataaaaacaa ttcatctgca ccggctccgg cataggcttt
     1621 agctatttca atgggatccc ccgcatcctt taaatcggcg aatttgaccc cctttacaac
     1681 tcgcccatcc cttatatcca aacaaggtat aatccttgta agcatttatt aaaccccctc
     1741 ataggctagg gcatctccaa gggctatagc accgctgtaa agcgctttac cgatgataac
     1801 accctccgcc ccgatccctt tgatcaattt tatatcttca agggaggtga cccccccgga
     1861 agctattatt ttaagccctg tctcatctat catggccttg gtcgcctcaa tattgggtcc
     1921 ctttagcata ccgtcctttt caatatccgt atatatgacg gtatctaccc ccatgtcata
     1981 tagcctcttg ccaaattcaa tactgtccgt ttccgttttt tctacccagc ctcgaattgc
     2041 gacctgtccg tcctttgcat cgatacccac cgcaatccta tctccataaa ttccaatggc
     2101 tttttcaagg agctggggct tcgttaccgc tgatgtgccc aatataaccc tggcaacctt
     2161 tggatcctct aaaaataatt ctatcttttt caaggtccta aagcctcccc ctatttgaag
     2221 ggagatggat acagactcta tcacttccat tataacttct atgttccgag attcaccctg
     2281 aaatgcccca tccagatcca caatatgtaa aaactttgcc cccatatcct gccatctcct
     2341 ggcaacctct accggattgt tactatatac ggtctccttg tcagcatcgc cctgcatgag
     2401 catgacgcat ctaccgtccc ttatatctat tgcgggataa atattcatct atacacctcc
     2461 aaaccttaat ccaatatgcc cttggtcgag gggataacat ccctgtctgc caaagtagaa
     2521 tcaacggcta cagcctgcct caaggctctg cccagggctt taaatatgcc ctctataata
     2581 tggtgggtat ttctgccata ggctactctc acgtgcagag ttaagcctag gtggttagag
     2641 aaggagcgta ggaattcctc cagtagctgg gtatcaaaat cgccgacaaa ttgggtcaaa
     2701 gccggcattt ccaggtgtag atatggtcta ccgcttatgt caaggctcac ctccgccaga
     2761 gtttcatcca tgggcagggt catattgcca tatctattta tgcccattcg ctgccccaat
     2821 gcctttttaa acgccctccc caacactata ccgatatctt caacgctatg atgtccatct
     2881 acctgcagat cccctatgca actgatatca atatcaaatc tcccgtggct tgataagaga
     2941 tctaacatat gatcaaaaaa acctataccg gttttgatat tattacgacc gctcccatct
     3001 atttctacag tagctgaaac cttggtctca aaggattctc ttttaactgt tcctgttctt
     3061 gccatattct tcttcccctt gtctatattt gatcctgttc cccggtgtag cgcccctgtc
     3121 gactaaactg cttgctacgg taatccccgc aaccaatccc ccataaaagg cgaattgata
     3181 ttcccctcat ctgtttcacc aattgaaatt agctccctat agaattttaa acatcgttac
     3241 gggtccttct attgttagaa atagaatata taccgcaatt tgtctaccct tctgagaaaa
     3301 tctcatacat ggaactaatg aatgcccggt tagcctcttc acaagctatg gtcaccctta
     3361 aacaatccct aaggtagggc tctcccgaaa agtcccttac caatattccc cggtcgagaa
     3421 gctgctgcca tgtcaaatgc ccacggggta ccttgataag gaggaaatta gcctgggaag
     3481 gataaatcct taaaccatct atttttccca aggcattgta cataatctgc ctctgctcta
     3541 ttattttctt cacccttata tccatctgtt ccctgttggc cagggccagt acagcagcat
     3601 cttgagaaaa ggtattcaaa ttatatgggg gcataaccct atttatttgg ctcgccaatt
     3661 ctggacttgt aatacaatat cctacgcgta accccgccaa gccaaaggcc ttggaaaagg
     3721 ttcttaagac cactaggtta ggaacctgta tagcatattc tatcatggag gctttagcga
     3781 attcgccata ggcctcatct atcaccacca cacccgggaa ggcattgatt agtttcttta
     3841 attgatcggg atccataagg tttcccgttg gattgtttgg attacagatg aataggacct
     3901 tagggttata tttggatatg gccgaagtaa aagcctccat atcatattcg tatccttccc
     3961 ctaactctat aggcatgggg gtagcccccg caattagagt aaagatctgg tacattccaa
     4021 aggacggggt agggtaagcc accccctcac ccttacctgc aaacccggta attaaaattt
     4081 gtatcagctc gtcagagccg gcaccgacta taacttgatc cgaggatact ccccaatgct
     4141 cccctatggc ccttcgtaaa tttgttgatt ttgaatcggg gtacctatta aaatccttcc
     4201 catccatcag atccctagcg agaatttccc taaaagagtc aggcaaatcc caggggctct
     4261 cgttagcatc catcttgtat atacacgatt tttcttcggc atcgtaggcc tttaaattca
     4321 tcagatccct tctaaggtaa ttgatcttca ttgtctaaac ctcgcttcta cggaccgggc
     4381 atgggcttca aggccctcta tcctggcaaa gtccgcaaca tctttatata gatcttccag
     4441 ggcctcttta gaaaaggata agatgctgga ttttttgata aaatcgtcca cggatagcgg
     4501 cgagaaatat ctggcgctcc cactggtagg tagtatatga tttggccctg ccatgtagtc
     4561 tcctaaaggt tcaggagaat aatggcctaa aaatatagat ccggcatttt taaccttgcc
     4621 cagcttttcg aatggattct cgatagccag ctctaaatgc tcaggggcaa tatagttact
     4681 aagttcaatg gccagctcta tattatccac taggattata gcgccaaaat tactcaagga
     4741 ttcctctacg atgtccagtt taggcagtgt tttggcctgt ctttcaatct cgcagcctat
     4801 attattgtaa aggtcaatat tatcggtgat gagtacggag gtagccaggg gatcatgctc
     4861 tgcctgggat aatagatccg ctgccacata ttccctgttt gcgctgctgt cagctatgac
     4921 taccacttcg ctgggcccgg cgatcatatc tatgtctaca taaccgtaga cctccttctt
     4981 ggccaaggta acatagatat ttccggggcc ggtgatctta tctaccttgg gaacgggttc
     5041 gatcccaaaa gccattgcag caatggattg ggcgcccccc atcttataga tgtccccaat
     5101 gcccgccata tccgcagcta caagggtata ggggttaaca acaccgccgg gccccggagg
     5161 ggtcgccata ataatattat ctacgccggc tatctctgcc ggaatcgcat tcataagaac
     5221 cgacgaagga tatgctgccc ttccccccgg cacatacatc cccacccact ccatgggctc
     5281 tattttttgc ccgagaacaa cccctgaatc atcctcaaag gatatccagc tgttctgttt
     5341 ttgcttctta tggtatctgg cgatcctatc catagcccgg tctattatat ctatataccc
     5401 cttagaaacc acggtatagg ctgttttgat ttcttcgccg gataccagca tcttttgggg
     5461 gtctttataa ttaacgccgt caaattgttc tatatattta aataatgccg ccgatccccc
     5521 cgactttatt tcggacaaaa tttcctttac tacattgcct tcctcgggaa agtccagttg
     5581 ggaaggtctc tttaaaaggg caataatctc agaactttcc gtacctctcc cgtcaatttg
     5641 cagtatcatt ctatccctcc tcaagcactc tttctataga ttcaatcaaa ttcttgatag
     5701 gctcgttctt agtcttaaag ctgatccggt ttaccactaa cctggcgcta atatcgcaga
     5761 tatcctccaa aaccaccaaa ccattttcct ttagggtacg accgctttct actatgtcga
     5821 cgataacatc ggaaaggcct accagtggac ccagctctac agagccgttg agctttatga
     5881 cctctatggt ttctccggcc tttgaaaagt agtttctggc aatattgggg tatttggtcg
     5941 ctactcgggt gtgggatttg gtaatccatg catccttctt ttcggggaac ccggcgaccg
     6001 ccatcttaca tttcccgaat ttaagatcca atacctcata gaggggtctt tcctcctcca
     6061 gcagggtatc cttcccggct atcccgatat cggctacccc gtattctaca tatgtgggaa
     6121 catcgctggg ttttactagt aagaatcgaa ttttctcgtt attggaatat atgatcagct
     6181 ttcttgatga aatatccaaa tcgtttaaat ttcctatagc cctttgtaac aattcaatag
     6241 ttctttctcc caatctcccc ttgggcaagg cgatggttat cacaatacac cctccctatc
     6301 ataatctttc atatctataa cctttaagat tccccttgct cttgcatatt caaaaggatc
     6361 ttggccctta tcggctttga ggtacacttc cactcgattt ccttcttccc taagcctttt
     6421 aattagatta tagccttccc tgcccctttc cctatcaggc accactaaaa tatcgatacc
     6481 cggtaatggc gtcaatttct tctgccgatc caaggccacc agaaggcgtt ttaatcccat
     6541 tgcgaaaccg gtagcaggca aatcgtatcc aaattcgcgt aaaaggttgt cgtaccgacc
     6601 ccctccacaa ataggaaatc ccaagttctt agtaaaccct ctaaaaataa tacccgtata
     6661 ataattaagg ctatgcacca tccctaaatc gaaggtgatg tattttgata atccaaagtc
     6721 gtctaaaatt tcatatactt catggatatt ctctatggcc tttaaacagc gtggattttt
     6781 agtatatcca agaccctgct ttaaaacatc aatatcgcca tacatagagg gtagctgcat
     6841 aaatatattc tttaaggcac catcaatagg aagttccccc aaaagggttt ctaaagccaa
     6901 caagttcttt tgctctatta aaaatcttag ctcctcacga acctccaagg ttaatcccgc
     6961 atcctccgtc aagcccttaa aaaagtcaac ctgtcctata tctatctgaa aatcctcgag
     7021 ccccagactt aagaatacct ctatagcggt agaaataact tctgcatcgg cctccggtcc
     7081 atccacaccg aacagctcga taccggcctg ggccacctcc cgctggtttc ctgtttgaag
     7141 cccctcgtat ctgtatacat tgcccagata tgatagccgg atgggaagca tggcatccct
     7201 tagttttgtc ccaaccatac gcgctatggg catagttata tcaggccgta agacttggat
     7261 cctaccccct gcttccacta ttttatacat tttctcctgc tctatatagg tcttatcccc
     7321 agtaaaaaca tcatagaact caaagacagg ggattcgatc tcatcatagc ccctgctaaa
     7381 gaacatttgc cttatggatt cctcgatttt ccccttattg taacattcct ctggcagata
     7441 gtcctgaacg ccttgcggtg tatgctgctt ccactttttc atttcaatcc ccattcagat
     7501 aatttagtgt tgtaaagtag taacttgata aatttattcg attgtattat actttcctgg
     7561 taatgtcaat ataaatttct gtatatgtta tgaaagaggc ctttcaccgc agaaccagtc
     7621 caattatctg gttttgcatt aaaacaatgc acttttcgta acatatgaat taagtgtaat
     7681 gttttacgtc actcgtatac tataggtaga caacatggaa cctagattta tcatgacgat
     7741 aattttttgt tggaactgtt tatctataat acagaatgtt tgtcgtaaca catatagaag
     7801 gggtcattaa gatatggact accatattca taccagatat tcctccgacg gggagatgac
     7861 aatagaagag gcttgtaaaa gggcaattaa aatagggctg aaggaaattg caattacaga
     7921 ccacattgat attgattggc cgaatcccga tattagtttt gatatagcca atatagacga
     7981 atatattcaa gaaatacatg acaataagga aaggtttaaa agtcaactta tcgtcaaagt
     8041 tggaatagag atagggctgc agccccacgt attggaagaa tccgctaaga ttatacggag
     8101 ccatcctttt gattttgtta tcggatcgat acatataatt aaagggatgg acccttatat
     8161 gggggactat ttttatggaa aaaccaaaag tgaaagctat gaaatctatt atagggagac
     8221 catagattta ataaaggaat ttgatgattt cgatgtctta ggtcatctag gatacataaa
     8281 aagatatttt ccccaaccct attcaaaaga ggatgagtac ctgcatcttg atcttgtaga
     8341 cgaaattttg gagatcttga tacaaaagaa caagggcata gaggtgaata catcgggata
     8401 caagcatagc tctgcatgtc ctatgccttc ccttaatacc attgcaagat ataaacagct
     8461 gggaggttct ataataaccg tcggttcgga tgcccacagt gttgatagca taggctatgg
     8521 aatagggcga gggaccaatg ccataaagaa tttgggtttt gattccgtta cttcttttac
     8581 ctcaagaaaa cccgaattcc gcagtattgg ggactgatcc caatattaat gaaaggtatt
     8641 gtgcaccccc gtatgatttc attgaaaatt atcgttacac catttgaaca tattccgcta
     8701 tatccaatcg accgcgcctt aatatctctt cgatatcctc tgatggcaag ggcctgctga
     8761 agagaaaccc ctgaatcata tcacatcgtt gatttaccag catacgcagc tgttcttctg
     8821 tttctactcc ctccgctgtc accataaggt tcatcctatg ggccaggtta atgatcacct
     8881 gtgcaatata ccttttatcc gaatcattgg ttatgtcctc tacaaaggat ctgtcgatct
     8941 ttaaattatt gataggtaaa cgatggagat agtttaggga ggagtatccg gtcccaaaat
     9001 catccagtgc aatccttacc cccatttgat acaactgttg aagcgcttct atattgtttt
     9061 ccgtggattc cattatatta ttttctgtta tttcaagctc caggtagctg ggatccagct
     9121 ccacatcctc cagaatgtcc ctgaccaagg aaataaaatt ccatcgcctc agctgaagtg
     9181 gagatatgtt aactgaaacc cggatagggt tgtacccctt atcctgccat ttcttatttt
     9241 gtttacaagc tgttctcaac acccattctc caattggtat aataagaccg gtatcctctg
     9301 caaaggggat aaagtaggca ggggggatga gcccatgctt ggggtggttc catctaatca
     9361 atgcctccac agcctcaacc cgcccagttt ttgaattaac ctggggttgg tataccaaat
     9421 ggaattcgtc cctatccaac gcatttcgaa gatctctatc catctccatc ctatggataa
     9481 ccctatcgtt catggatttt tcaaaaaatt cgtaggtgtt tttaccatac ttctttgcct
     9541 catacatggc gatatcggcg ttttttaata gtacctctgt cgtatcccca tcatcgggaa
     9601 acatggtaat accgacacta accgtaggat acacctcaaa tccatcaata acccacgggg
     9661 catcaaagat tcctaaaatg ctgcttatgg tattctctac gtcttgccta tcctctatcc
     9721 caaatctgac catgataaat tcgtcaccgc caaacctgcc aaccaggcct ttgccttcgc
     9781 tgatcttagc cagccctgtt ccgaatctct tgaggacctt gtccccaaat ccatggccga
     9841 atgtatcatt tatcaccttg aaattatcga tgtctaaaaa aaacaaggca ccttttttat
     9901 tctcccgctt tgcacttgca atccagtggg ttatccgatc cgaaacgttt agcctgttag
     9961 gaagacccgt caccgcatca tagtatgcta aatagcgtat cctctcctgg ctaaccttca
    10021 gagccccctc ctggtgctca attttgtgtt tacgataatg gagttcctcc tccatatccc
    10081 ctatttcttc gtatatagcc atgagttctt gatgcttggc cataagttcc tgatatcggt
    10141 gctctgattt agtctgttct tccaacagag taatttgatc atacccaatc tgagcaaata
    10201 gcttggctat ccgggatgcg aactccataa acctatccat ctcctctttg gaatagacag
    10261 gcaccttttt aatggcttct atgtattctc cctcgggtag ccccagggtc cgtgcctggg
    10321 ccttaaagta ttctaaatcc ggatcggata gaaagaattg tgatttataa ataaccgcta
    10381 cacatttatc cttgatgtag atgggggtaa tggcatgcat aattccgttc tcgcatttat
    10441 agtaaacata tccctcgccc tttgcaattg cctttgctgc caattccggt gcgcttttag
    10501 tggattcaac gcatctgttt gctatacggg gatcccctct atgaaacctt gtacatacat
    10561 cggtccagcc tgtcctagtt aaaactatac cgttagcgtc ggcgatacca gccgctattc
    10621 ccgtagcctt atatatactg tcaaataact cttgaaacct atcaatatcc actaaatccg
    10681 aaaattcaca attcacgcca ccagccacct tacatgttta gtttagtaat gattatatat
    10741 tttatatgag actccttgta tcggaattta cgaaatagga atcatcttgt tcctaacgcg
    10801 ttacggaaaa tacattgttt taaaaacaag atcagaagat acaagtggtc ctgtaacgaa
    10861 aggcccccgt aacgcataca attataatac aaaatcccct aaaatggaat actccctcag
    10921 tgctattttc gacaattacg ctgtctatta atgcgaaatg aacctatccc agcaataggt
    10981 ccgtgtaatg cactattttt aaaggcgcta tctatgatct tacaccggag gaagctctac
    11041 ggattttatc ataaaacgac aattgcccaa gggggtatct acagtgaccc tatcccctac
    11101 ccctttaaat agaagggcgt agcctacggg ggacagacaa gatgcacaat caagtcctcc
    11161 tccaccatca ccatagaagg ggggtactat ctttagtctt tctatttcgc tatagtcaag
    11221 atcctggact tccgcaacgc tccctattaa gattagaggc catcgttgtt ttacagagac
    11281 ctcgggattt gcatcaataa atccctttaa aaaatccaga tattggtcca tcatgccctt
    11341 aaaagcatcc ctttccccgg tatgttcttc gtagaattcc tttaaaatat gattcctggt
    11401 gcttcttatg gtcttcaaat gttttgttaa aaaatcggaa atctgatgcc gatgataatt
    11461 ctcctgcatt gctatcctcc ttgtaataaa acgattactt taataaacgc tactcaataa
    11521 aataacgaac aggcccacgg tgccgtcaac ttttatggga tacgaaacaa ggcatcccat
    11581 ggacccccac gaaatgcctt ccatgacaat attaccatag gtgtaaatca ttgtcaatgg
    11641 tggtaaatta tctcctattt ctcgattatc tcgctgagta tttcagctat gtatttggcg
    11701 ctgcgcttgg cttcggcaaa ggtagtcaag tggtttccca cctcaattat gatggcccta
    11761 ttggacacat gttgattata ccgtcccgtc tgcagcttta ctttatttgc cagtccggga
    11821 tatttttcat tggccttgtt ggtaatctta aggccgaatt gatagttttc ccgccattca
    11881 ggtttctggc taaacccgcc gctcacccct tgggcagtac ctatgaggac gaaaaacttt
    11941 gccacccgtt ccccgtctat gatggttact tccttatccg gatctttgag gccttttata
    12001 taattacgat gaatatctaa atagatcttt agggatttat attctttctg tcttttctta
    12061 acggtttcta acgatcgggg ataaagcccc gatttccaag gatatacctc atgatccgtg
    12121 gtatcgtgaa gcacctgtat gccccgattc tgtagctgat tggtcaattc ctcccctact
    12181 ccaacgaccg tttgactaaa atcagtgctt ctgaaaactg ctatatcctt atatggattg
    12241 ttaggatcct gtctgtaggc ctcccttgaa tgggtatgat agatcagaat ctggggccca
    12301 tcacccttca aggcgatagg cggaaggtca tcactgattt ttttgacctg gatctgtatc
    12361 tcttcagaaa cttctcccac agcctcctcg taacgctcaa gatccagatc cgttggccga
    12421 ttggcctggc gtgaagtctc ctgctccggg gtggcaactg ccgcaaccac ggattcagcc
    12481 ccattttccg catatatagg ttcattttgg gcataggcag cgctgagata aggcatgtga
    12541 gaggataata taaaggctgg atcggtaaag ttcatctcct ttaatcggat ccatatatcc
    12601 gagaaaaaat cacccttatt cccagatatg taggcatcgc ttttctcttt aatattcgtg
    12661 gattcatcgg ggaacccgtc acccttccct aggaaggctt catggttgga tagcagacga
    12721 taactgagaa gggcaatccc gattattaat aaaatgacaa ctaggttata tgacaaattc
    12781 gattttttgt ttattcttat tctctccata atccccacca cttcctatag caattagttt
    12841 atttgcccga tatacccatt tggcccatat atatccttag tcaattgtct aaataaaacc
    12901 ttgtttatga cccatattgt agataaacaa ttccaactat aaaatctaac ggctatagat
    12961 agatctagtt tccgtatttt ccatctataa tatagcgctc taataaatat attagactac
    13021 tatgggggaa tatgactggt atggcagtac ttttaaaaac attggaagca attgcattgg
    13081 caattgcaac acacaggttt caacgaggcg gaggatttgt acccaccgcc tgttttacta
    13141 atgggaactt gacgcttata gaagcgggag acatcttgcc gactcgttct atttacaaag
    13201 cgagggcgac catttacggt cgccctcagc taaaaatcaa cttttactac ctaaagtttg
    13261 gcccacccct gtcccctgtt ccgggcaacg ggagtaagct ttttcaaatc ataatctatg
    13321 ggtcttgatt gtcaaaccga ttccaaaaga ccctaatcat attatttgaa gatcctcctt
    13381 gcagtaatca gacgcttgtt gtaagagctt aaattactga tttttacccc atccttctta
    13441 ttggatgcat ggatgaattg attgcctcca atataaaggc ctacatgccc tgagaagcaa
    13501 agtatatctc cgggttttaa gttttcctta gatacaccgc tgcccactga ccgctgtgat
    13561 ttggaactgt ggggaagact taccccaaat tggctatata cgtacttggt aaacccggaa
    13621 caatcaaagg tatttggtcc ggaagtcccg aaaacgtact tgtagccctt gtatttcatc
    13681 gcaaaatcca ctacagaact cgccgagccg ctgacggtac ccctatcggt tgtggtatta
    13741 ccggatgact ttggtttgga agtggatttg gaatttgtag caggtcttct gacgggcttt
    13801 ggtctaggag ctggtttggg tttctccttg gtccccttag caataatatg cttaaccggt
    13861 tccttttcca ccttctcctc tattatatcc cgggagacct cttgtccatc ttctctggtc
    13921 accagggcga caatatcttt tttgccgttt tcgccctgct ggactacctt ggttttccct
    13981 ttttccaaac cgttatcttc tttcgtctcg gtttcaaagg gtatttcttt agagtagcga
    14041 accttttcct tcgttacagt tgtcaacagc ttcttttgtt ggccaatcat aataatgtct
    14101 tccggctgaa ttatatcccc atccagatcg ggattggcaa gttccaaatc cgaaacgctt
    14161 acatggttct gttgggctat tgaccacagg gagtcacctt cctgtaccct gtactcttta
    14221 atctcctggc cgccgtgcag gatcgcctct gcggccttct caggactgag gatatttttc
    14281 acagaagtga taccctcctg aaaatcgata tcctgtttaa acgcaacatc ctccagttgg
    14341 ctgtcctctt tttcctttat tttttctatg tatggggcct taatattata aatcacttcc
    14401 tgggcgtctt ccatcgtctt tacatagcat acccgtgtcc cgtcgatatc tatagaatag
    14461 gcatcgaccc ttatatcaat taactcttca atggaattag ccaattcatc atcgtcggtt
    14521 atttcgtctg cccctacatt tgtttcctta aaatagatct tggaatcaat aaatacttcc
    14581 tcctggtatt ctcctctttg cctcctttct atatcgtcat aaattgacaa cgctctggcc
    14641 gggaacttga ctattcccac ctgctcatcg ttgatgtaca ttgtgtagac ggtcgatgaa
    14701 ccccccgtat aggcaagtgc ggggattgtc accccagtta acaatagagt caacagcacc
    14761 gtacacaata aaatcttaaa gcttttcata gtaacctcca tcttgaacag cctttttatt
    14821 acggttctat ctcgaacctt tacggaattg ttacgttatt attctatata ttctacataa
    14881 atgttaaata tccttctttt cataggtatt atatccaaaa actttttttt aattaaagtt
    14941 ccgttgcctt tccagctcat acaacataat ggaggcagcg caggctatat ttaatgaggt
    15001 aatatgccca tgcatgggta ttctgacaag aacgtcacat agctgcttgt agttgtagct
    15061 aaggcccagg gtttcattgc ctatgatcag tgccataggt cttgttgcca aatccgcatc
    15121 cataataagt gcatccccgc ctgctgatga accaactatc ttaaaatccc tatggatttt
    15181 cctgatatca ttaaaccagg gtaacagctg tctgtgtgat tgcaacctaa ttatgggaac
    15241 cgcaaacaag gttcccatac tggcccttat ggtcctggaa tcgtatatat cgacggcatg
    15301 tcccgtaaca acgatgccgt caaccttaag tgcctcacat gagcgtataa tcgttccgag
    15361 gtttccgtaa ccggagggcc tgtcaataac caccagtagg gtatcatccc ctacccttat
    15421 tcgagcgata tcatcacccg gaatggatac aatggcaatg agctccgata attcctcatc
    15481 tttatcgctg agcttatcca ttaacctaag gggcagttca aaatgggttt tggccgtaga
    15541 atttcggagc accccttttg cccaatcgga taggggtgcc tccccggaat atacaaaaga
    15601 ccggatctcc caattgtttt ctatggcaaa gttgatgggc tttacccctt cgacaaagaa
    15661 ttccctatat ctgtttctct tttccctatt cctttttact acttcaatcc gctgaaaatc
    15721 gttattctcg ttacggattg tcttgacaag cggtttcatc tatctactcc taaaattatc
    15781 gtgatatttt tggcggggaa taagtatggg cataacgggc tattttttta aatgaattaa
    15841 cccaccttga gattctcaga taaacagctt caactattaa atttatcagc tgtagataaa
    15901 ttaaattccc gtattgctta tctttagatg agtgctaagt atttgcgcag attctcaagc
    15961 ccgatcccaa cgcccgcttt cggatcttcc atcccctcaa actcaatgga caatgcacct
    16021 ttatagcctg aatccttagc tatcctgagg cattgcgcta ttgctacatc cccgtgtcca
    16081 attatggcgc ccctcagata attcccgccc ctggacctaa accagccttg ccccggatca
    16141 gggtgcatgc ccgatttaat atggaagtcc ttggcatgaa catggaaggc gtagggcatc
    16201 agtctgccca ttgccgtctc gggactttca tcgacacaga caaagttgcc catatccagc
    16261 aatagcccga aattgggatg gtctactccg ttgattaact tttctaccct atcgctgtcc
    16321 tgacagaaaa aaccgtgatt ttctatcatg gtttggatac caaattcctg ggcgtattct
    16381 gtaacagccc tgcagccttc tatcaacctt ggaagagccg catcgaaatc ccttggtccc
    16441 gtatgctcct tagaaaatcc gacagtagca tcatgcctca tggtcttaga gccgagaagc
    16501 cttgcaatgt ccacctcacc cttgactctt tgaatttccg catccaagtc cccgtccgag
    16561 ccgtttataa aatccgcacc caccgtatag cttcccatct ctatgcctac tctggcacat
    16621 tctgccttgg cccttacagc gaagtcctca ttggtttcgt tctcttccaa tataaagggc
    16681 acaaattcaa tgagatcaca acctaaatcc ttggctaaag aaataacatc gatctgcttt
    16741 atttgtccgc tgcgtaccag gttgattaaa ctataggagc tcacacctaa cttcataata
    16801 ttcccccctc gtataatatg attatagggt ttaatttatt ttagcctgcc acaccaggca
    16861 attgtaccta tgccgataaa ttcagtttcc atattgctta tctatagata aacaactcca
    16921 actataaatt ttttcaccat gaaattgcat agacccattg caactatcct accgaaaaat
    16981 tcagttttcg tattgcttat ctatagattc tataccatgt cccacgcttt ttccacaata
    17041 actggtaaaa actttttttc ttgacttttc ccatggattt ggtaatatag ttgtgtattg
    17101 tgatgatggg aaggagtagt tgaatcccct agccaaagcg acccgggggg agtgagagct
    17161 cggggctatg tttcaatgaa gggcgtctcg gagcattttt atg
//
LOCUS       DASRNX010000145         4920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_295505,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000145 DASRNX010000000
VERSION     DASRNX010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295505"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFD89_05980"
     CDS             complement(<1..711)
                     /locus_tag="VFD89_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HZJ57776.1"
                     /translation="MDKESCFEIIREHLEAKQFIIDNKREISYGIQFEIIHELERGIF
                     RIYEGKKGIRLDSSQIRHAGLRESIERIIKSTMAGKSNIIGATDVGLGNDRINKPLMA
                     AKEAESVINVVQDPKQIIGIDESGKGDYFGPLVIAGVYTDEKTGPQLKHAGVADSKKI
                     TDNRIRELAMFIKDTCPYDIVVIENERYNDLYSAIKNLNRLLAWGHARVIENLLERVH
                     CEHALSDQFGRPELIQKAL"
     gene            complement(728..1552)
                     /gene="proC"
                     /locus_tag="VFD89_05985"
     CDS             complement(728..1552)
                     /gene="proC"
                     /locus_tag="VFD89_05985"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZJ57777.1"
                     /translation="MPTYNLGVIGAGNMASSIVKGVVKNKLFDPKQIFVFDILSHQLT
                     KLADETSVSAAKDNIDLLEKCSVVIIAVKPEALDALLDEISDVITPNHVLISIAAGIS
                     TEYIKDGIGNKCRVVRVMPNTPAMVGEGMSAICNNHGLDHEELEAISTIFSSLGRIEF
                     VDETLINGVTAISGSSPAYVYMFIEALADGGVMMGLPRDQSYRMAAQAVLGAAKMVLD
                     SNMHPGSLKDMVSSPGGTTIEAIYTLEKNGFRGSIMEAVRNCALKGASMDKEGRDR"
     gene            1852..1927
                     /locus_tag="VFD89_05990"
     tRNA            1852..1927
                     /locus_tag="VFD89_05990"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1885..1887,aa:Trp,seq:cca)
     gene            complement(2010..3203)
                     /locus_tag="VFD89_05995"
     CDS             complement(2010..3203)
                     /locus_tag="VFD89_05995"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZJ57778.1"
                     /translation="MELSKKNQGITASLTLEIDAKAKRMKAQGMDVIGFGAGEPDFNT
                     PSFIIEAAKEALDKGYTRYTPSSGIMELRQSICHKLLRDNGVDYKPNEILVSNGAKHS
                     LYNVFQAILNPGDEVIIPSPYWVSYPEMVRMADGIPVFVDTLEEENFGINPDRLKEVI
                     TDRTKAIIINSPNNPCGCVYDREQLEGIAELAIEHGFFIVSDEIYEELLYEGSQHLSI
                     ASLDPQVRDITLLINGMSKAYAMTGWRIGYTAGNPKIIDVMANVQSHATSNPNSIAQY
                     ASLAALNGAKDIIKDMVSQFDDRRKFMVDKINRIDGIKCTTPLGAFYVMMNINDVLKK
                     EHNGRTLGGSLGFADALLESKNVAVVPGIAFGADGHVRLSYATSKDNIGEGLCRIENF
                     IQELV"
     gene            complement(3324..4259)
                     /locus_tag="VFD89_06000"
     CDS             complement(3324..4259)
                     /locus_tag="VFD89_06000"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZJ57779.1"
                     /translation="MKIIDGHCDTLTHLLDKSSSMDDISNHVNIDALIMGGVNIQFFA
                     AWIGPRQRYGSPLQRGIKLIDRYYTMLDEYSAIFVPVFRYADVKGIKHNQIGSLLTVE
                     GGDILEGEIANLRVLHKLGVRLMTLTWNNRNEISDGIMETSSRSGLSRFGASVVREMN
                     RLNMLIDLSHISVRGFWDAIEHSSDPVIATHSNARAICSHPRNLDDDQILAISEVGGI
                     VGINFYPPFLTDEKEAHIEDIIRHIEYISGLVGIDSLGFGSDFDGIEQLPEDVGGPGD
                     FPKIIERLLRLNYSERDVQRICSDNHLRVIKRVLK"
     gene            complement(4417..4677)
                     /locus_tag="VFD89_06005"
     CDS             complement(4417..4677)
                     /locus_tag="VFD89_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HZJ57780.1"
                     /translation="MEVLKVSSRSSPNSVAGALAGVLRERGSAELQAIGAGALNQAIK
                     AVAIARGFVAPGGLDLVCIPAFTDIIIDGEERTAMKLIVEPR"
BASE COUNT         1315 a         1196 c          877 g         1532 t
ORIGIN      
        1 tagagctttt tgaatcaatt caggtctccc aaattgatcc gataaagcat gctcacaatg
       61 aaccctttct aaaaggtttt ctatgaccct ggcgtgtccc caggccaata atctatttaa
      121 gttcttaatc gctgaatata agtcgttata tctttcgttt tctatgacaa ctatatcata
      181 tggacaggtg tccttaataa acatggccaa ttcccggatc cgattgtccg ttatcttttt
      241 gctgtctgct accccggcat gtttcaactg gggcccagtt ttttcatcgg tatagactcc
      301 ggcaataacc aatggaccaa aataatcccc tttccccgat tcatctatgc ctataatttg
      361 tttagggtct tgcacaacgt tgatgacact ttctgcctct ttcgccgcca ttaaaggctt
      421 gttgatccta tcgttcccta gacctacgtc agttgcacct ataatatttg atttccccgc
      481 cattgtggat ttaatgatcc tctcgatgct ttccctaagg ccagcatggc gaatctggga
      541 ggaatctagc cgtatgccct ttttaccctc atatattcta aaaatgccac gttctagctc
      601 atgtattatt tcaaattgta ttccatagct tatttccctc ttattatcta taatgaattg
      661 tttggcctct aaatgctccc tgatgatttc aaaacagctt tccttatcca tagaattcag
      721 atacccccta cctatcccga ccttctttgt ccatggaggc ccctttaagg gcgcaattcc
      781 gtacggcctc cataatgcta cccctaaaac cgttcttttc caacgtatat atagcctcta
      841 tggtcgtgcc ccccggggag cttaccatat cctttaggga tccgggatgc atatttgaat
      901 ccagcaccat ctttgccgca cccaataccg cctgggcggc catcctgtag gactgatccc
      961 gaggcaggcc catcatgaca ccgccgtcag ccagtgcctc tatgaacata tagacatatg
     1021 ccgggctgct gccgctaatt gcggtcactc cgttgattaa agtttcgtcc acaaattcga
     1081 tcctccccaa ggaagaaaat atagtgctga tcgcttctaa ttcttcatgg tctaaaccat
     1141 ggttattgca tatcgcactc atcccctccc ccaccatcgc aggcgtgttg ggcataaccc
     1201 tgacgaccct acacttattc cctattccat cctttatgta ttccgtagaa atacccgcag
     1261 caatggagat tagaacatga ttgggtgtga ttacatcaga aatctcatcc agcaatgcgt
     1321 cgagggcttc aggtttaacc gcgataataa caacggaaca tttctcaagc agatcaatat
     1381 tgtcctttgc ggcagacaca gaagtttcat cggctaactt tgttagctgg tgcgacagaa
     1441 tatcgaatac aaatatttgt ttcggatcaa ataatttgtt ttttacaact cctttaacta
     1501 tggaagaagc catatttccg gcccctataa cccctaaatt ataagttggc atcaatacca
     1561 tctcctatca gtaatctata tgttctacga caagcatccc atattgatat acaggtgtcg
     1621 taaaacatta cccttaattc atatgttatg aaaaacacat agtttcatca caaaatcaga
     1681 gaatttagct ggttttgtga tgggagactt tttcgtaaca tatctagatt ataccatatt
     1741 atgagcgaga ttttgccgga tagcccagtt agcacatgaa aatattcaaa acctattgac
     1801 ttatccggac agttatccta taatattgaa tgtgcctaaa aacataattt taggggagta
     1861 gctcaattgg tagagtagtg gtctccaaaa ccattggttg cgggttcgat tcctgtctcc
     1921 cctgccataa gtaaagagtg gatttcatcc actctttttt gtatctaagt attcaatcat
     1981 tagagcgaat ttaaaaggat ttatcccggt tataccaact cctgtatgaa attctctata
     2041 cggcatagcc cttcgcctat gttatccttg gaggtagcat aggaaagcct gacatgtcca
     2101 tcggcaccga aggcgattcc gggcaccaca gctacattct ttgattctaa cagggcatcc
     2161 gcaaaaccaa gggagccgcc caaggttcgg ccgttatgtt cttttttaag gacgtcattg
     2221 atattcatca taacataaaa agcgcccaag ggtgtagtac acttgatacc atcaatcctg
     2281 ttgattttgt ccaccataaa ctttcttcta tcatcaaatt gggagaccat atccttgatt
     2341 atatcctttg caccatttag ggccgccaag ctggcgtatt gagcaatgga gttggggttt
     2401 gaggtcgcat ggctctgtac attggccatt acatcgatta tctttggatt ccccgctgta
     2461 tagcctatac gccaacctgt catggcgtag gccttagaca tcccatttat caatagggta
     2521 atatctctta cctgtgggtc caatgaagca atgctcaaat gctgggaccc ttcgtataac
     2581 agctcttcat agatctcatc tgatacgata aaaaagccgt gctctatagc caactctgca
     2641 atgccttcca gttgttccct gtcataaaca cacccgcaag gattattagg gctattaatg
     2701 attatagcct tggtcctatc ggttataact tcctttaatc tgtctgggtt aataccgaag
     2761 ttttcctctt ccagggtatc aacaaatacg ggtataccgt cggccattct aaccatttcc
     2821 gggtagctta cccaataggg tgagggaata ataacctcgt cccccgggtt taatatagcc
     2881 tggaagacat tgtaaaggga gtgttttgcg ccgttggata ccagtatttc attgggcttg
     2941 taatcaaccc cgttgtccct tagcagctta tggcatatgg attgcctcaa ttccattatc
     3001 cccgatgatg gggtatacct ggtataaccc ttatccaacg cttcctttgc agcttctata
     3061 ataaatgagg gtgtattaaa atccggttca cccgcaccga aacctattac atccattccc
     3121 tgggccttca ttctctttgc tttagcatct atttctaatg tcaatgacgc agtaataccc
     3181 tggttctttt ttgatagctc cattcattct gcctcccaaa atgaaagatt gttttctgtt
     3241 ccatcatttt cttcccatta taccatatca tatgcctaca gtccacgaca aagtgaagga
     3301 ttgttatatt tattttgcat taatcatttt aggacccttt taataacccg taaatgatta
     3361 tcgctgcaaa tcctttgaac atccctttcg gaataattta atcgtaacaa tctttcaata
     3421 attttgggaa aatccccggg cccgccgaca tcctcgggca attgctctat cccatcaaaa
     3481 tccgatccaa atcccaggga gtctatgcca acaaggcctg atatatactc aatatgccgt
     3541 attatatctt cgatatgtgc ctctttctcg tcagttaaaa aaggcggata aaaattaatc
     3601 cccactatgc ccccaacctc ggatatagcc agtatttggt cgtcgtctaa atttctagga
     3661 tgcgaacaga tagcccttgc attggaatgg gttgcaataa ctggatccga tgaatgctca
     3721 atcgcatccc aaaacccccg aaccgatata tgtgacaggt ctataagcat attcaatctg
     3781 ttcatttctc ggacgacgga tgctccgaat cttgataatc cgctgcggct gctggtttcc
     3841 attatcccat cacttatttc atttcgattg ttccaggtca aggtcatgag gcgaaccccc
     3901 aatttgtgta acactcttag gttagctatc tctccttcta ggatatcacc gccttcgacg
     3961 gtgagtaaag acccaatctg attatgcttt atccctttaa catccgcata tcgaaatacc
     4021 ggcacaaaga tagctgagta ttcatccagc atagtatagt acctatcgat taattttata
     4081 cctctttgaa ggggtgaacc atacctttgc cttgggccta tccatgcagc aaagaattga
     4141 atgtttacgc ctcccataat gagtgcgtca atgttaacat gattcgagat gtcatccatg
     4201 ctactggatt tatccaatag atgggttaaa gtgtcgcaat gcccgtcaat tattttcata
     4261 aattaacccc atttcttctg caaatgatca gaagcaaagc agatagctat ctaatctcat
     4321 aataaaacca aggattcaaa acccattaac gatggaataa ttattttatt taaccctttt
     4381 caagttcaac aaaaaaccta ccattagacg gtaggtttat cggggttcaa ctatgagttt
     4441 cattgcagtt cgttcttcac catcgattat gatatcagta aaagctggga tgcaaaccaa
     4501 atccaatccc ccgggagcaa caaatcctct cgcaatagct actgctttta ttgcctggtt
     4561 taaagcacct gcgccaatgg cttgcaattc cgctgacccg cgttctctaa gcacaccggc
     4621 caaggccccc gccactgaat tcggactaga tcttgatgat actttcaata cttccatgat
     4681 ttgaccccct cattcattgg tgtatgctta taatattcta catcttcgca ggaaatcctt
     4741 cttttaagac tcttttgctt taaatgtcgg cgaaatacaa aaggtgtagc tgtgctacac
     4801 cttattttgc atattcaatg gctctagttt cacgaataac gttaatttta atttgtcctg
     4861 ggtattctaa ttctttttca acacttttta caatgtctct agccattagg atggcttgat
//
LOCUS       DASRNX010000146         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_559908,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000146 DASRNX010000000
VERSION     DASRNX010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559908"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /gene="spoIIIAC"
                     /locus_tag="VFD89_06010"
     CDS             complement(<1..186)
                     /gene="spoIIIAC"
                     /locus_tag="VFD89_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HZJ57781.1"
                     /translation="MNVDIIFKIAAVGILVAVLNQVLLRAGREEMAMLTTLTGVVVVL
                     LMVVNLINNLFNSIKSIF"
     gene            complement(211..732)
                     /locus_tag="VFD89_06015"
     CDS             complement(211..732)
                     /locus_tag="VFD89_06015"
                     /inference="COORDINATES: protein motif:HMM:NF021090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HZJ57782.1"
                     /translation="MIIKLISSIAIIVCCSFLGFHMSYRYTQRINNIRDLRNALSYLE
                     TEIVHYSTLLVHAIKNSSISVMGDWKPFFHEIAELLEGRVECSLAEVWQQSLKALEHN
                     PYIDKAEYEIMQRFGLQLGNSDKSSQEKYFQLAQEQLKAEEKSAREARVKYSRMYYNL
                     GIIVGLGIAIILF"
     gene            complement(799..1788)
                     /gene="spoIIIAA"
                     /locus_tag="VFD89_06020"
     CDS             complement(799..1788)
                     /gene="spoIIIAA"
                     /locus_tag="VFD89_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HZJ57783.1"
                     /translation="MKQKMDDLGYVIQPVLDALPDNLRGIIMSVSQVVLKGLEEIRVR
                     QGRPLMVHSHGRDYFLCRNGGVTRHLQNAYIVSANDARRILELISDYSIYAFEEELSS
                     GYITLQGGYRVGLSGKMTSDKKGTPKFQYITSFNIRIAREIYNVANKIIPYAVKGNRV
                     FHTLILSPPQMGKTTLIRDLARQLSNGFPGFSGVKVAIVDERSEIAGCYQGMPQNDVG
                     VRTDILDGCPKATGIIMLIRSMSPDVIITDEIGRPEDVTAIEDALNAGITIITTAHSH
                     GLEDARRRPILKSMLESNIFQRIMVLGNSLGTGTLERVYDPIEGKELLDLPIR"
     gene            complement(2043..2441)
                     /locus_tag="VFD89_06025"
     CDS             complement(2043..2441)
                     /locus_tag="VFD89_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57784.1"
                     /translation="MDYLMEKVAYLKGLMDGLEIDDRTNEGKILINVIDILHDIVHEI
                     GDLNASKNELDEYIQTIDEDLSDVEDELYGSDDDDEYTDDLHYIEVECPHCHETVYFA
                     DDLFDTEDELHCPNCNESIYDEEAPPELDS"
     gene            complement(2520..>2653)
                     /locus_tag="VFD89_06030"
     CDS             complement(2520..>2653)
                     /locus_tag="VFD89_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006556749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZJ57785.1"
                     /translation="DTATGGSKPATLETGAVVNVPLFVDVGDMIRVDTRSGEYMERA"
BASE COUNT          703 a          656 c          476 g          818 t
ORIGIN      
        1 gaatatactc tttatgctgt taaacaggtt attaattaga ttaactacca taaggagcac
       61 gaccaccaca cctgtaagcg ttgttaacat ggccatttcc tcccggccgg ctcttaaaag
      121 gacctgattt aatacagcca ccaatattcc cacagcggct attttaaaaa taatatcaac
      181 gttcacgtcc tcttcctcca ttcgtttatg ctaaaagagt attatagcga tgcccaggcc
      241 cactataatc cccaaattat agtacatcct gctatacttc actcgagctt cccgggcact
      301 tttttcctct gctttcaatt gctcctgggc gagttgaaag tatttttcct ggctgctctt
      361 atcgctgtta cccagctgaa gcccgaacct ttgcattatt tcatattccg ctttatcgat
      421 ataaggatta tgttccaaag cctttaggga ttgttgccat acctctgcca gggagcattc
      481 cactcttccc tccagcaatt cagcgatctc gtggaaaaac ggtttccaat cgcccataac
      541 ggatatgctg gaatttttta ttgcatggac caatagagtg gaatagtgaa caatctcagt
      601 ctccaagtaa gataatgcat ttcgcagatc cctgatgttg tttatccttt gggtatagcg
      661 ataggacata tggaacccta agaaggagca acaaacgata atagcgatac tggaaattag
      721 ttttattatc atactgtttt tagcccccca ttaatagact ttcgtatatg gcattgtgca
      781 attaaaaagg atcacaacct atctaatagg cagatccaat agctcttttc cttctatcgg
      841 atcatagacc ctttccaggg tccccgtccc aagactattt ccaagaacca ttatccgctg
      901 aaatatattg ctttcaagca tagattttag aatcggtctc ctcctggcat cctccaatcc
      961 atgactatgg gcagtggtta ttattgtgat accggcattt agcgcatcct ctattgcagt
     1021 aacatcctca ggccttccta tctcatcggt tatgataaca tccggagaca tggaacggat
     1081 gagcataata atccccgtgg cctttgggca tccgtctagt atatccgtcc ttacacccac
     1141 atcgttttgg ggcataccct gatagcatcc cgcgatttcc gagcgctcgt cgactatagc
     1201 taccttaaca cctgagaaac cgggaaaccc attgctgagc tgtctggcta aatcccgtat
     1261 cagagttgtt ttccccattt ggggcggaga cagaatgagg gtatgaaata cccgattccc
     1321 tttaacggcg taagggatta ttttgttggc tacattatat atttcccttg ctattcttat
     1381 attgaaggat gttatatatt gaaacttggg tgttcccttc ttatcgctgg tcatcttacc
     1441 cgataatcct accctatatc ccccctgaag cgttatatac ccgctactga gctcctcttc
     1501 aaaggcataa atagaatagt cggaaatcaa ttccaatatg cgcctcgcat catttgctga
     1561 aactatatag gcgttttgaa ggtggcgggt tacgcccccg ttccggcata gaaaatagtc
     1621 ccttccatgg ctgtgtacca tgaggggacg cccttgacga actctgattt cctctagtcc
     1681 ctttagcact acctgggaaa ccgacatgat gatccctctt aaattgtccg gtaaagcatc
     1741 caatacgggt tgaatgacat atcccaaatc atccatcttt tgtttcatct taactcgtcc
     1801 tttccatatg tataatatac ctatgttcat gtggactagt ttatacttga ttgcaataaa
     1861 aaaagaccgg caataaccgg tcaggataac tacatgtcaa aagatccctc tttttgtaaa
     1921 tgggggatcc ccgtcagcta aataggctgc aaaacagatc ccccattagg tggaatttag
     1981 aatgctgttt atatctgtac ataatgatct aacatatcat atggtcggtg tgtaggacct
     2041 tattaactgt ccagttccgg gggggcctct tcgtcatata tggattcgtt acaattaggg
     2101 caatgcaatt catcttccgt atcaaaaaga tcatcggcaa agtagaccgt ttcatggcaa
     2161 tgtggacatt ccacttctat gtagtgtaaa tcatcggtgt attcatcgtc atcgtcgcta
     2221 ccgtataatt cgtcctccac atccgataaa tcctcatcaa tggtttggat atactcatcc
     2281 aattcgttct ttgaggcatt cagatcacca atttcatgca cgatgtcatg cagaatatcg
     2341 atcacattta ttagtatctt tccctcattg gtgcggtcgt caatttccaa tccatccatt
     2401 aatcccttta gataggcaac cttctccatc aaataatcca taattcatcc tcttattcta
     2461 tacattattt atttaaaacc cataaatttg ggcttatggc tctgtcaata tgattcccct
     2521 caagcccttt ccatatactc tccgcttctg gtatctaccc gtatcatatc ccctacatcg
     2581 acaaacagag gtacattaac aaccgcacct gtttcaaggg tggcaggttt gctccccccc
     2641 gtagctgtat ccc
//
LOCUS       DASRNX010000147         7865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_348442,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000147 DASRNX010000000
VERSION     DASRNX010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348442"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..1774
                     /gene="spoIVA"
                     /locus_tag="VFD89_06035"
     CDS             296..1774
                     /gene="spoIVA"
                     /locus_tag="VFD89_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HZJ57786.1"
                     /translation="MEKFDLYRDIAERTDGDIYMGVVGPVRTGKSTLIKRIMDLLVLP
                     NIENEHKKERAIDEMPQSGAGRTIMTTQPKFVPDEAIEITIRENASLKVRMVDCVGYL
                     VKGAMGHMEGDSPRMVRTPWFDYDIPFEEAAELGTRKVITDHSTIGLVVTTDGSITDI
                     SRSNYIEAEERVVRELKELDKPFVMILNSKTPDVPETINMREALEEKYDVPVLLLDVM
                     NLTLEDIDNILSNVLFEFPLTEIRIDIPKWVQSLDDDHWLIGHIFDSIGEFTRDVARV
                     RDVDRIKDALPDSEYVKAPTITEISLGNGKVLVDMDTKPGMFYRVLGEISGYNIQGDY
                     QLVGLLKDLAVAKREYDRVADALDDVRNTGYGTVSPAMEELVLEEPEIVKQGGRFGVR
                     LRASAPSLHFIRADIQTEVSPIVGSEKQSEELINYLLDEFESDPAKLWESNIFGKSLH
                     ELVKEGLANKLTRMPEDAQFKIQETLQRIINDGSGGLICIIL"
     gene            2244..4322
                     /locus_tag="VFD89_06040"
     CDS             2244..4322
                     /locus_tag="VFD89_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZJ57787.1"
                     /translation="MSKHKNRDEPGIKNTDFPISIGKRVKRPRAGNGIYIVISILIFM
                     FSLAYVCIYNCIGRAQQHFVENGVLDLRDWDGLGKRNIRLTGEWEFYPGELKEPSDMP
                     NSAKGSFDIHDGDEDIGYIKVPGDWSPYLNHRGTPDGAGTYRLVIRVPEDGIYGIKTE
                     TIRTAGRVFLNGDEIITMGKPAITRTDFVPSSRYAMGFSKSKEGIVELIIQVSSFGYP
                     SGGILKDIRFGSIETIVNHVNRERAIEAFTIAMSLITGIMFLFVFFQRGYETYQGYFS
                     MGNIFMALYLSTMNNQLLSLILNYDLILRLRIQIFTMTAVALCSLRFMHHFLRDFSNK
                     RVVRVLSIIGLSVFIFVINDPRRAISFRVLTGQIYVSLTMIIIYAYSSWILLKAIKDG
                     TGFLKYIILITSSLTVYWFSMMSKILFEFHIGNLPQYMILVTVLGIVLLINARLHAEF
                     KEVSQLTEERLEKEFKYFYSQISPHFLFNTINTIIALSFVDTEKTRKALNNLSVYFRG
                     KLELHMEKGLIPLEEELDMVIAYLEMEKLRYGDELLFEVDIEGDLAAVIPPLTLQPLV
                     ENAVNHGIKPKEGPGTIKLTVKYAGREQIEISIEDDGVGMTPEKRAQLIRGDLKRLGF
                     KNIIRKIKILKGFSIDVQSALNIGTTIRITLPGVKGHENFDIGTVSLKALSEPRLFGA
                     SGLYDRHKGS"
     gene            4356..5255
                     /locus_tag="VFD89_06045"
     CDS             4356..5255
                     /locus_tag="VFD89_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZJ57788.1"
                     /translation="MKVILVDDEPAALMVLSSLLSKYEDIEILGSYTCPMDALEASKK
                     IRPDVVFLDIEMGKVNGLEAAELFSGQGRQEIVFITAYSQYAVEAFEVNALDYLLKPI
                     QERRLHRTIQRLKTKFKDDMLLGVEDNETQDSLRNGLIISSLGNFEVSNLKGENIYWR
                     TQKTKELFAYLWIHNGTYINRSMIMEVIFPDKNLSSATALLHTTIYQLRKALESLGFS
                     DGIVYTNESYMLQLPIESDVEDLNSIIGLNSYSHKEIGAILEIYRGDFLEQEGYSWAI
                     GIQESLRDAVYAILEAYAFLQLD"
     gene            5295..5483
                     /locus_tag="VFD89_06050"
     CDS             5295..5483
                     /locus_tag="VFD89_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57789.1"
                     /translation="MYKLNPFCETMAKMMIEFFGIQRKKPELKKFFKKFADELWEEMG
                     IKPMKSTADLYNEFLNSL"
     gene            5969..6646
                     /locus_tag="VFD89_06055"
     CDS             5969..6646
                     /locus_tag="VFD89_06055"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZJ57790.1"
                     /translation="MEEKQVYTMPLIGEDAPSFKAITTQGEINFPEDYEGKWVILFSH
                     PADFTPVCTTEFMTFATMAEEFRELNTELVGLSVDSLYAHIAWLRKIEELEWNGMKNV
                     EVKFPLIEDIKMDVANKYGMIQPGQSNTQAVRAVFIIDPEAKIRTILYYPLSMGRNFD
                     EIKRIILALQKADKDNVATPADWRPGDDVIIPTAGSCGTAKERMESQDDDMYCLDWFM
                     CFRREKK"
     gene            7121..>7865
                     /locus_tag="VFD89_06060"
     CDS             7121..>7865
                     /locus_tag="VFD89_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZJ57791.1"
                     /translation="MDTKKYDKPSFISTKRGYLFLLIGISLILVGLPMIHHHVGPILF
                     AHIDPLTFHYIGMVISQFLTVSILAIILWKNRSLEERNISMIRFLRETNDKNIDLLKE
                     QERIMGDLEDSRRMYQDIIEFLPDATFVVDNHDRVLTWNHAMDHLTGIKEGDILGKDQ
                     SVYSVPFYGKPHNMLINMVNSPTLDTIEGYNNLVQTGLSISADRYVSNLNQGRGAYLW
                     STASPLFDGQGNKIGAIQSLRDITHLKQTE"
BASE COUNT         2448 a         1480 c         1857 g         2080 t
ORIGIN      
        1 agggtacgga gaataagata acatctacag gcggacctag gggactaaag taatcaatga
       61 agtcatccgc tattaagtag ctcataacta ttatcatgcg gcaagataat gagttagccc
      121 gacatgataa gtttagttga cctattgttt atctataatt tatgacaact tgtcataaac
      181 cttttcccat tccaatatac attattatag atatttatgt atgctgatgt tgtactgata
      241 tctttggtaa tccaatggtt atcataacca tttaataaca gggagggaaa aggtgatgga
      301 gaaattcgac ttatatcggg atattgccga acgtaccgat ggcgatatat acatgggagt
      361 ggtagggccg gttagaacag gtaaatccac tctgataaag aggattatgg atttactggt
      421 tctcccgaat attgagaacg agcacaagaa ggagagagcc atcgatgaga tgccccaaag
      481 cggtgcaggg cgtactatta tgaccaccca acccaagttt gtgcccgatg aagcaatcga
      541 gatcactatc cgtgagaacg ccagcctaaa ggtaagaatg gtggactgtg tcggatactt
      601 ggtaaagggt gccatggggc atatggaagg ggatagccct aggatggttc gcaccccctg
      661 gttcgactat gacatcccct ttgaagaggc cgcggagctg ggtaccagaa aggtgataac
      721 agaccattct accatcggat tggtggtaac taccgacggg agcatcacgg atatttcccg
      781 atccaactac atagaagccg aggaaagggt ggtaagggag ctcaaggaac tggataaacc
      841 ctttgtgatg atattaaatt ccaagacccc tgatgtcccc gaaaccatta atatgcggga
      901 ggccttggag gaaaagtatg acgttcccgt tttattattg gatgtaatga acctaaccct
      961 tgaggatata gacaatatcc tgagcaatgt gctcttcgaa ttcccattga cggaaatccg
     1021 catagatata ccaaaatggg tgcagagcct agacgacgac cattggctca tcgggcatat
     1081 atttgattcc ataggggaat tcacccgaga tgttgcaagg gtaagggatg tggataggat
     1141 taaggacgcc cttcccgatt ccgaatatgt aaaggccccg accatcaccg aaataagcct
     1201 gggaaacggc aaggttttgg tggatatgga tacgaaaccc gggatgttct acagggtttt
     1261 aggggagata agcggttata atatccaggg tgactaccag cttgtggggt tattaaagga
     1321 tctggccgtg gcaaagaggg aatatgacag ggtagcagac gccttggatg acgtcagaaa
     1381 taccggctat gggacagtat cccctgccat ggaagaattg gtattggagg agccggaaat
     1441 agtcaagcag ggcggcagat tcggggtacg cctgcgggcc agtgcgcctt ccctgcattt
     1501 cataagggcg gacatacaga ccgaggtctc gccaatagtc ggcagcgaaa agcagagcga
     1561 ggaacttata aactacctat tggatgagtt tgaatctgat cctgccaagc tttgggaatc
     1621 caatatattc ggaaaatccc ttcatgaact ggtgaaggaa ggccttgcca ataagctgac
     1681 taggatgccg gaggatgccc agtttaagat ccaggagacc ctccagcgca ttatcaacga
     1741 cggcagcggg ggactcatct gcataattct gtgatagacg gctcaaggca aatcacagtg
     1801 cagctgggat attgatcccc ttttgccata caagaacgac gaaaacctgg tttatactgg
     1861 ttcgaatatt atatagttga actcataacc tactaggtgt taaaatggta ttaccaaagt
     1921 acacctgttg ggtatggggt tcaactatat tgctttttgg aaagcctaac cctttactct
     1981 ccctctcatg tatgtcatcg tatgaaattc aactatattg cttttttagg gacacctttt
     2041 ttgctttaac cacagacaga caatgcggaa actaaattta tcggcataac gattgcggcg
     2101 catctataca atttcatggt gttaaatttt atagctgggg ttgtttatct ataatcccgt
     2161 gtttcaaata agaggacggg ggttatcaaa ggatagtgat aatgagagaa tttttctgaa
     2221 gctttttgta aggggtagta gatatgagca agcataagaa tagggatgaa ccggggataa
     2281 aaaacacaga ctttccaata tccattggga aaagggttaa gcggccaagg gccggaaatg
     2341 ggatatacat agttatttca attctaatat tcatgttttc acttgcctat gtttgcatat
     2401 ataattgtat cggtcgggca caacagcatt tcgttgaaaa tggggtactg gacctcagag
     2461 attgggacgg attagggaaa aggaatataa gacttacagg ggaatgggaa ttttatcccg
     2521 gtgagctgaa agaaccatct gatatgccca actctgccaa agggtctttt gatatccatg
     2581 atggcgatga agacatagga tatataaagg tcccggggga ttggagccca tatctaaacc
     2641 atcgaggtac cccggatggt gcgggaacct atcgcttggt gataagggtg ccagaagacg
     2701 gcatctacgg aataaagacg gagaccatca ggacggcagg ccgggtattc ttaaacggtg
     2761 atgagattat aaccatgggg aaacccgcca taaccagaac tgattttgtg ccttcaagta
     2821 ggtatgccat ggggttttca aagagcaagg aaggcattgt agagcttata atacaggtat
     2881 ccagctttgg atacccttcg ggggggatac tgaaggacat caggtttgga tccattgaaa
     2941 caatagtaaa tcatgtaaat cgggaaaggg caatagaggc ctttaccatc gctatgagcc
     3001 ttataactgg aataatgttt ttatttgtat tttttcaaag gggttatgaa acctatcagg
     3061 ggtatttcag catgggcaat atatttatgg ccttatacct atcgactatg aacaatcagc
     3121 ttctcagtct gattctcaat tatgatctaa tcttgagact gaggatacaa atatttacaa
     3181 tgactgctgt cgcactctgc tctctgcggt tcatgcatca ttttctgagg gactttagca
     3241 ataagagagt ggttagggta ctgagcataa taggcctttc tgtgttcatc tttgttatca
     3301 atgatccgag aagagccata tcgtttcggg tcttaaccgg acagatttat gtaagtctta
     3361 ccatgataat tatatatgcc tattcatctt ggatcttact gaaggccatt aaagatggga
     3421 caggctttct aaaatatata atactgatta ccagctcgtt aacggtctat tggttcagca
     3481 tgatgtctaa gatcctgttt gaatttcaca tcgggaacct tccccaatat atgatactag
     3541 ttactgtgct gggcattgtc ctattgataa acgccaggct tcacgcagaa ttcaaagagg
     3601 tttcccaatt gacggaggaa aggctggaaa aagaatttaa atacttttat tcccagatat
     3661 ccccgcactt tttattcaac accataaata ccataatagc cctcagcttt gtagatacgg
     3721 aaaagaccag gaaggcactt aacaacctct ccgtatattt caggggaaaa ttagagctgc
     3781 atatggaaaa ggggctcatc cccctagagg aggaattgga catggtgatc gcgtatcttg
     3841 agatggaaaa gctgagatat ggggatgaac tattatttga ggttgatatc gaaggggatt
     3901 tggcggctgt gataccccca ttaaccctgc aacccttggt tgaaaatgca gtaaaccatg
     3961 ggataaagcc caaagaaggg cctggaacaa tcaaactcac agttaaatac gcaggccggg
     4021 aacagataga aataagtata gaggacgatg gggtgggcat gacaccggaa aaacgcgccc
     4081 aattgattag gggcgattta aaaaggctgg gatttaaaaa tatcatcaga aagataaaaa
     4141 ttctcaaggg gttttcaatc gatgtacaaa gcgcgttgaa cataggcaca accataagga
     4201 taaccctgcc cggggtaaag ggccatgaaa actttgatat agggacggta tccctaaaag
     4261 ctctgtctga gccccgcctt tttggggcat ctgggctcta tgacagacat aaaggatctt
     4321 gaagataaag cgatattatc aaaaggggtg aggcaatgaa agtaatattg gtagatgacg
     4381 aaccggcggc tttaatggtt ctgtcgtctc ttctttcaaa atatgaggat attgaaattt
     4441 tgggcagcta tacatgcccc atggacgctt tggaggcgtc caagaaaata aggcctgatg
     4501 tagtgttttt ggatatcgag atggggaagg taaacggatt ggaggctgcg gaactgttca
     4561 gcggccaggg caggcaggaa atagtcttta taacagcata ctctcaatat gctgttgagg
     4621 ccttcgaagt aaatgctttg gactatctat taaaacctat acaggaaaga cggctccata
     4681 gaaccataca aaggctcaag acaaaattca aagatgatat gctcttgggc gtagaagata
     4741 atgaaactca agattcgctc agaaatggat tgatcatatc aagccttggc aactttgagg
     4801 tgtccaacct aaagggcgaa aatatatatt ggcgtacaca aaaaacaaag gagttatttg
     4861 catacctttg gattcataac gggacataca taaatagatc aatgataatg gaggttattt
     4921 tcccggataa gaatctatcc agtgcaactg ctcttttaca tacgaccata taccaactca
     4981 gaaaggcctt ggaatcatta ggattttccg atggtatagt atatacaaat gagagctata
     5041 tgctacaatt gcccattgaa agcgatgtag aggacctgaa ttctattatt ggattaaaca
     5101 gctattctca taaggagata ggagccattc tagaaatcta taggggcgat tttttagaac
     5161 aggaaggcta tagctgggct ataggcatcc aggagagcct aagagatgca gtatatgcca
     5221 ttttggaggc gtatgccttt ctgcaattgg actaaaaaga aatcaacgaa acgttgagta
     5281 agtgtttaga attaatgtac aaactaaatc ctttttgtga aaccatggct aagatgatga
     5341 tagaattctt cgggattcag cgaaaaaagc cggaactaaa aaagtttttc aagaaatttg
     5401 ccgatgagct atgggaagag atgggcatta agcctatgaa aagtacagct gatctctaca
     5461 atgaattttt gaactcatta tgatggtttc cagtatcatc aatacaggca tagcgataaa
     5521 tcgggaatat tgtgtatggg aaaatatatt tgattaagta gaatataatt cagcagacta
     5581 tggaccagat gaggatttgg taatgatatt taaaacgctg cggcccaaac aattcgtatg
     5641 tttaagcccc gtatcttgtg tgtatatata gatatagggt cctttaggaa taactaaaca
     5701 tcagaaacga ttgcccttaa tgattacatc caacgtttct atggaacggg aaatatctgt
     5761 ctgccgactg tttaactagc gagagcccgc catttattta ggcgtggtat agattccagc
     5821 cccgccgcaa ttatgttgga tgcaatcaaa ctacataatt taggacctaa tggaaacctt
     5881 tcaaaaaatg atccattacc tttttgaaag accaacaatc agctttataa acgataatga
     5941 cataacaaaa gatgaggagg aaagtatcat ggaagaaaaa caagtttaca cgatgccatt
     6001 gattggcgag gacgcaccta gctttaaagc cattaccacc cagggagaaa ttaattttcc
     6061 ggaggattac gaagggaaat gggtaattct attctcccat ccggccgatt ttaccccggt
     6121 ttgtaccacc gaatttatga catttgccac tatggctgaa gaatttaggg agcttaatac
     6181 cgaactggta ggcctttccg tcgattcgct ctatgcgcat atcgcatggc ttcgcaagat
     6241 agaagagctg gaatggaacg ggatgaagaa cgtagaggta aagttcccct taattgagga
     6301 catcaagatg gatgtagcaa acaaatatgg tatgattcag ccgggtcaat ccaataccca
     6361 agcggtaagg gcggtcttta taatcgaccc cgaagcaaag atccgtacca ttctctatta
     6421 tccattatcc atgggtagaa actttgacga aatcaagcgc attatcctgg ctctacaaaa
     6481 agctgataag gacaatgttg caactcccgc ggattggaga ccaggggatg atgttattat
     6541 ccctacagct ggttcatgcg gcactgccaa ggagagaatg gaaagtcagg acgacgatat
     6601 gtactgccta gattggttca tgtgctttag gcgagagaag aaatagttac ggatttgcgg
     6661 taaggcaatg cctaatcgtt tgatcccaca ctctttgtaa aaaacagccc taatcccaac
     6721 ggattacggc tgttttttgc tatattggag tatctagggg caaaccgatc acgtataaaa
     6781 gcatgatact ttacagttga atatagcctt taggtgggat ccactgccgg taccgtatat
     6841 ccaggaacgt tccaagagat actttatcca tttttgccat cagttaaatt acatatcaat
     6901 cggaggttat cggctatagt atacagtatg taagcactgg gttaaatata tgggaaaaat
     6961 gttctaaaat gtttgaataa ttctataatt cgacatataa tattggcaaa ggtctataag
     7021 gactacaagc ataatccggg gaagagtatt ggtggccggg aaaaataata taaaggggta
     7081 gcacaatgaa aaacatcaaa gtttggggtg aaaatatcgg atggatacaa aaaaatatga
     7141 caaacctagt tttatttcta ccaagagggg atatctattc ttattaattg gtatatcact
     7201 tatcctagta gggctaccga tgatccatca ccatgtaggg cctatcctat ttgcccatat
     7261 agaccctctt acattccatt atataggtat ggttattagt caattcctca ctgtttccat
     7321 actggcaatt attctctgga aaaatcggag tttagaggag cggaatatat ccatgatccg
     7381 cttcttacgg gagactaacg ataaaaatat tgacctattg aaagagcagg aaagaataat
     7441 gggggacctg gaggattcca ggagaatgta tcaggatata atcgaattct tgccggatgc
     7501 tacctttgtc gtggacaacc acgaccgcgt tttgacatgg aatcacgcca tggaccattt
     7561 aaccgggatt aaagaagggg atatactggg caaggatcaa tccgtgtatt ctgtcccctt
     7621 ttatgggaaa ccgcataata tgttaataaa tatggtcaac agccccaccc ttgatactat
     7681 tgaaggatat aataatcttg tacagacggg attaagtatc agtgcggacc gatatgtgtc
     7741 caacctaaac caaggcaggg gagcctacct ttggtccacc gcatcccccc tctttgacgg
     7801 tcagggcaac aagataggag ccattcaatc ccttagggat atcacccatc taaaacagac
     7861 tgagg
//
LOCUS       DASRNX010000148         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_771527,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000148 DASRNX010000000
VERSION     DASRNX010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771527"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VFD89_06065"
     CDS             complement(<1..835)
                     /locus_tag="VFD89_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZJ57792.1"
                     /translation="MRPYVKRPIIWITISYMIGVLAEGSIKWKFCYPYIALGAAAIFV
                     GLVKLRRRGLYLPLVLLIFTLAGILNTYIHSIPNTDLDDLIGQAPVTYGQIMEKVKQS
                     DSSASFILEARGIDHNNAQYGVNCKIRMTVYFSPDEGDDPGIDIGDWVEIRGKIERPQ
                     GRRNPKGFDYRSYLGRRGIYYTMGIPGGNVLSIKAGKLPWPNSWLRSAGDYMARVFNT
                     YVGGGESGLIKAMLIGEKWALPYRVTEEFRNTGIAHILAISGLHIGFIILLLSWLTGI
                     LK"
     gene            complement(919..2037)
                     /locus_tag="VFD89_06070"
     CDS             complement(919..2037)
                     /locus_tag="VFD89_06070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HZJ57793.1"
                     /translation="MDQRESEDLTPAGEGAVDGSLEGQDTGEDHEEQGDMEPIVDEPI
                     EEPPDEPLEEPPEEPIEEPPEDIVITISLAGDCTIGTDETFTYTNSFPDRLEKVDGDW
                     SYFFAGVVDIFENDDLTLVNLETTFTKVTKKADKRFRFKGDPSYVNILKEGSVEMVNL
                     SNNHIYDYLQRGFDDTIETLDDAGILYSGEGYKAFYESQGITMASLGYQGWNTNIKDR
                     VKKDIEEVRDKADIVIVSFHGGIERDNYPNSVQKELGRFAIDEGADIVAGHHPHVIQG
                     IEEYKDKYIIYSLGNFCFGGNRNPDDKDTFIFQSQFTFTDGELKESQGNIIPCSVSSR
                     KDVNDYQPTVLEGEEAERVMGRILKYSSKLKYGIGTDD"
BASE COUNT          730 a          690 c          537 g          824 t
ORIGIN      
        1 actttaagat tccggtaagc caagataata atagaatgat aaatcctata tgcagtcctg
       61 atatggccag aatatgtgca atccccgtgt tcctaaattc ctctgtaacc ctatatggta
      121 atgcccattt ttcgcctatg agcattgcct ttatcagtcc ggattccccg ccccctacat
      181 atgtattaaa gacccgggcc atataatccc cggcggatct taaccaagaa tttggccagg
      241 ggagttttcc ggcctttatg gataaaacat ttcccccggg aatacccatg gtataataga
      301 tccccctgcg tccgagatag gacctataat caaagccttt gggatttcta cggccttggg
      361 gtctttctat ttttcccctt atctctaccc aatcacctat atctatcccg gggtcatccc
      421 cctcatccgg ggagaaatag accgtcatcc taattttgca attgacccca tattgggcat
      481 tgttgtggtc tatcccccgg gcctccaaga taaaggacgc actagaatcg ctttgtttta
      541 ccttttccat aatctgtcca taggtcacgg gggcttgtcc aataagatca tccaaatccg
      601 tattgggtat gctgtggata tatgtgttga ggatacccgc taaagtaaat atgagcaaga
      661 ccaggggcag atacaaaccc ctgcgtctta attttacaag cccgacaaat atagctgctg
      721 ccccaagggc tatgtatgga tagcaaaact tccatttaat agaaccttca gccagcactc
      781 caatcatgta agagattgtt atccatataa taggacgctt tacgtacggc ctcatacttt
      841 cccccaaatt tgttttcccg gataaattga tctattatat gcagataccg ccaaagccgc
      901 ctcctatggg ggcctttgtc aatcatccgt cccgatcccg tatttcagtt tactgctgta
      961 ttttagtatt cggcccatta ccctttcggc ctcctcgcct tccagcacag tcggctgata
     1021 atcgtttacg tcctttctgg atgaaacgct gcagggaatg atattgcctt ggctctcctt
     1081 aagctcccca tccgtgaagg taaattggct ctgaaatata aatgtatcct tgtcatcggg
     1141 gttccgattg cccccaaagc aaaaattgcc caggctgtag attatatatt tatccttgta
     1201 ttcttcaatg ccctgaatca catggggatg atgacccgcc acaatatcgg cgccctcatc
     1261 aatggcaaac ctgcccagct ccttctgtac gctgtttgga taattgtccc gctcgatgcc
     1321 cccatggaag gagacgataa ctatatcggc tttatccctg acctcttcta tgtccttttt
     1381 aacccggtcc ttgatgttgg tattccaacc ttggtatccc agactagcca tggttatccc
     1441 ctgggattca taaaaagcct tatagccttc gccggaatag agtattccgg catcgtccaa
     1501 ggtttctatg gtatcatcaa agcccctttg cagatagtca tagatatggt tgtttgaaag
     1561 gtttaccatt tctacgctgc cttcttttaa gatattgaca taggagggat cacccttaaa
     1621 tctaaacctc ttatccgcct ttttagtaac ctttgtaaag gtagtctcca aatttaccaa
     1681 ggtaaggtcg tcattttcaa agatatccac taccccggcg aagaaatagc tccaatcgcc
     1741 atcgaccttt tccaaacgat ccggaaaaga gttggtgtag gtaaaggttt catcagtacc
     1801 tatggtacaa tcccccgcca aagaaattgt gatgacgata tcctccggcg gctcttctat
     1861 gggctcttcc ggtggttctt caagcggttc atccggcggt tcttctatgg gctcatcgac
     1921 tataggttcc atatcacctt gttcttcatg gtcttccccg gtatcttgtc cctccaatga
     1981 tccgtcaacg gccccttccc cggccggtgt cagatcctcc gattctcgtt gatccattaa
     2041 gccctccgtt atatatgtgc aagaagtaaa aaaaaccaga caaagacaaa gggttatgat
     2101 caaaaataat atatagcggt gcattcattt cacgtccttc ttcatttact gcaatcaccc
     2161 cagcccatac aaaccctaga aacgtatcgc cgggaatatg ccctttctat tccatatgca
     2221 gtcccgaata taacgtctgc ataatgtttc catattctac atcattgaca aatatcctct
     2281 ttattagtcc aaattcttcc catgtttttg tatcacttgg tagctgtctg agcgcagcga
     2341 gttcgggaag gtcccgaggc aagcttacag gttatttacc ttggaactta agcgatggga
     2401 atattatttt cctggttcgg attattcgcc caatagaaca tgaaaaattg cgaattatca
     2461 gtagataata tataatgggt atgttacgaa aaagattatc atcttaagtc agctaagcca
     2521 tttggttttg cactaaaaca acgtgttttc gtaacctatg taataaagaa gctgttctac
     2581 gatgcctgcg ccagggtgca ggatctttat agtcgaacat gtagatgccg gacagccaaa
     2641 tataatttca aagtccaagg ggaagtgatt atcatcaggg attcattgtt tagaaattca
     2701 atgcataaaa atttgaccct ggatcaagtg tacgaaatgc tcgtgcaatt tttaaggcaa
     2761 gccccacaca acgaatatcg t
//
LOCUS       DASRNX010000149         8339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_716634,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000149 DASRNX010000000
VERSION     DASRNX010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8339
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716634"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFD89_06075"
     CDS             <1..301
                     /locus_tag="VFD89_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57794.1"
                     /translation="LPYLMEGGINCLFPFEVNGCSHPAELFAEYGKELRIMGGVDKIE
                     LGKGPEAIKAYLETLVPLVERGGYIPFCDHRCPPNVRPQDYLYYLDLKEKMFGLT"
     gene            complement(312..1553)
                     /locus_tag="VFD89_06080"
     CDS             complement(312..1553)
                     /locus_tag="VFD89_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57795.1"
                     /translation="MKIESYEIGILRELAKEYAEVAALDIHRVNKERMRDNNDLKLGR
                     PPVLLDEVPWHEMDIDGQLINRCKGDFAVNMETYFRRRLFQWKYFPGDMVVENFYPIN
                     KSIHSTGYKPETKENIAITDAANNIISHQYIDQLETEADLEKLTIPHITGDKGADKRS
                     VEMAQDILGGILPVKLRGYYLYYAPWDEIPRLRGVSNIFYDMMDRPEFTHKIIQRFTE
                     IQLSTMEQYEEQGLLDYDLADLHCTPPYSGDLPAVDYDGGKVRLKDIWIRSMAQMFSS
                     ISPEQHEEFDLQYSRPLFEKCGLVYYGCCEPLENKMELLKRVPNMRKLGVSPWSDQEK
                     SAEQIGGDYVYARKPNPASVAVKTDPDEIRKDIKETVEICLKYGCPYEFVLKDISTVS
                     YRPENLIVWEQTVKEVLDSYY"
     gene            complement(1696..2292)
                     /locus_tag="VFD89_06085"
     CDS             complement(1696..2292)
                     /locus_tag="VFD89_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZJ57796.1"
                     /translation="MDSERLKKQIDFIVEVDKLKNIYRQTLLMDGSRNENDAEHSWHL
                     ALMAMVLKEHSPKKDLDITKVLKMLLVHDLVEIYAGDTFCYDEKANLDKAHRELVAAE
                     RLYSLLPSDQGQELRALWDEFEEKETPEALFASSLDRLQPLICNYHTGGHTWVKYGIK
                     SSKVIERNLPIKEGAPSLWELVQDMVEDSIEQGILERD"
     gene            complement(2323..2478)
                     /locus_tag="VFD89_06090"
     CDS             complement(2323..2478)
                     /locus_tag="VFD89_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57797.1"
                     /translation="MADFITIVIAILAIVLIMKIAAKILKFVLTIAVIGVVLYVLTNY
                     GFLNGLF"
     gene            2863..4305
                     /locus_tag="VFD89_06095"
     CDS             2863..4305
                     /locus_tag="VFD89_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003490811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZJ57798.1"
                     /translation="MFSNICLGDHRPLYLQIRDYIKRMILKGLLGQGQKLPSTRELCD
                     TLGVSRNTVILAYEYLEDEGFVQIVRGRGTFVSDINIDYQSVWSMDWTPRLTPYADLA
                     NELDIVKHEAKWQKGMISFKSIAPDDNLFDLEDFKKAFLNRFSLEGEKLLNYGYAQGY
                     KPLIDYLTHYMENKGVNIQGKSILITNGFTEGFDIALSALTRPGDHILCENPTHNTAI
                     KIMKLHGLKIVGVDINEDGIDLNILKEALSNNSISMGFFIPSYHNPTGIVMSPEKRVE
                     VLKIFGRYRVPIIEDGFNEELRYSGSHLSPLAALCGRGNSIIYIGSLSKVLFPGLRIG
                     WILGDSELISRLESIKRSRNIHSSFLDQAILYEYLAAGNLEKYLKKARRIYREKYDYA
                     LECAKRYIPCQRVLGEGGLHIFIELEGNLNARKVLDKCIRENVIFMPGDIFYIHGKGA
                     NTFRLGFARSSLEEIEKGFKIIGNTVRGLS"
     gene            complement(4364..5575)
                     /locus_tag="VFD89_06100"
     CDS             complement(4364..5575)
                     /locus_tag="VFD89_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57799.1"
                     /translation="MEKKNLVINAAVCDARNVSETVLESYKDISINAAMVLVSQETKD
                     LLAKHNVAMNTAEVTEVSKDVEIMVQNGSYEIKDSTLLSKPVILIVNGSLNIETRSQE
                     VLDKFTHIQVNGLVSYPDNIKDRLPIIKVNGSTDSYPGDAIRLRSELVIDKTFILRAK
                     GEKYYVRNKVVMSDKDLNVASLNEMGTKFITKEAIIVESLLEGALPLFDEHAEIKTIP
                     DGLVYTEGQVLNNVLIRKHGDRLYVDGDLTIGPESEAALDDLTKAKIDGTVFISSRLV
                     DKFHDIDVEYNAIEETKGHRMGDRAFLTIDKRKLDKYADGIRVFDCGIVNIKDDIDSD
                     EIEEKLEFVDCGVINCSEDQKSAVETVSKGVGMITDSQKGKLDTLKGLMGGLGMGDKD
                     TKIINAASYTM"
     gene            complement(5581..6045)
                     /locus_tag="VFD89_06105"
     CDS             complement(5581..6045)
                     /locus_tag="VFD89_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZJ57800.1"
                     /translation="MINKLYKNYRNKLLWYCLNLSRNNPSIAEDIVQETFMKALENTH
                     ILNDLTDAQCRSWLYRAAKNIFIDRVRRVANAPKIEEPLFKEDDLSKIIVSQLCAGLP
                     DKERTLFWMRYIEGYTSRELGELFNLSPSTVRSRLSSARRKMSEIYYRERER"
     gene            6239..6421
                     /locus_tag="VFD89_06110"
     CDS             6239..6421
                     /locus_tag="VFD89_06110"
                     /inference="COORDINATES: protein motif:HMM:NF020945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HZJ57801.1"
                     /translation="MSEFFELNQKIEILRGKLNELLSDKNPVSKDRIIRYSRELDELI
                     AKYHAIENMQGPTEPL"
     gene            complement(6430..6600)
                     /locus_tag="VFD89_06115"
     CDS             complement(6430..6600)
                     /locus_tag="VFD89_06115"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZJ57802.1"
                     /translation="MAASKHLSNTYQIEEKLKGLCVKFINVLKDAYDSGILTEDEYLE
                     HVKTKEEFLKKG"
     gene            complement(6893..7777)
                     /locus_tag="VFD89_06120"
     CDS             complement(6893..7777)
                     /locus_tag="VFD89_06120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZJ57803.1"
                     /translation="MQELDIVLDKLHIFEKLYDVMRIVDPIRKKVLELKGDQFFDADI
                     PCYKLWKKRELCENCISIRAFNEDDAIYRLEHKEGHIYMFTGIPVIIHGKRLVVELLK
                     EATKSLIMGIDKESQGSEAYTMIEYMNRIAIRDPLTDLYNRRYINERLPVDLLNASTR
                     GEPLSIILADIDFFKNINDTYGHAIGDEILKEFAGQLKGYIREGKDWVARYGGEEFLI
                     CLSNTDTKGAKKVAERMRRGIEKKQFIIDGGAISLTCSFGVYMVYGGAEDMTVEDVVG
                     LVDKRLYKAKRLGRNRVQ"
     gene            8114..>8339
                     /locus_tag="VFD89_06125"
     CDS             8114..>8339
                     /locus_tag="VFD89_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57804.1"
                     /translation="MEPVSKTPDGQTLIETHRHCKFIEASVSLSEKTIRRWDPYFCNL
                     KFVLIFLVILGHLIEKHIYVNHYLYIIYTVI"
BASE COUNT         2323 a         1896 c         1483 g         2637 t
ORIGIN      
        1 ccttccgtac cttatggagg gcggtatcaa ctgtttgttc ccctttgaag taaatggttg
       61 ctcccatcct gcggaactat ttgccgagta tggcaaagaa ctgcgaataa tgggaggcgt
      121 cgataagatc gagctgggca agggccctga ggcaataaag gcctacttag aaaccctggt
      181 gccccttgtt gaacgagggg gctatattcc attctgcgat caccgttgcc ccccaaacgt
      241 aaggccccag gattatctgt actaccttga cctaaaggaa aagatgtttg gactgaccta
      301 atacagatcc gtcaataata tgaatctagg acttctttga cggtctgctc ccatactatc
      361 aggttttcag gccgatatga aacggtgctg atatccttta ggacaaactc ataagggcag
      421 ccgtacttta ggcagatttc aacggtctct ttaatatcct ttcggatttc atccggatcg
      481 gtcttcacag ctacgctggc ggggttgggc tttcgcgcat atacataatc cccgcctatt
      541 tgttctgcac tcttttcctg atctgaccag gggctgaccc ctaattttct catattgggc
      601 acccttttta agagttccat cttgttttcc agaggctcgc aacagccgta gtataccagc
      661 ccgcattttt caaagagggg tctgctgtat tgtagatcga attcttcatg ctgctccgga
      721 gagatagatg agaacatctg tgccatactg cgaatccaaa tatccttcag ccgtaccttt
      781 cccccgtcat aatccactgc aggcaaatcc cctgaatatg gaggcgtgca atgaaggtcc
      841 gccagatcat aatccagcag tccctgctcc tcatattgct ccatggtgga caactgtatt
      901 tcagtaaacc tttgaattat cttatgggta aactccgggc gatccatcat atcgtagaat
      961 atattggata ccccccttag cctgggaatc tcgtcccatg gggcatagta aaggtagtat
     1021 ccccgtaatt taaccggcag gatacccccc aggatatctt gagccatctc cacacttctc
     1081 ttgtcagcgc ccttatcgcc cgttatatgg ggaatagtaa gcttctcaag gtcggcctcg
     1141 gtttccaatt gatcgatgta ttggtgggag atgatattat tggctgcatc ggttatggct
     1201 atgttctctt tggtttccgg cttatatccg gtagagtgaa tggatttatt gattgggtag
     1261 aaattttcca ccaccatatc cccgggaaag tacttccatt gaaatagcct tctcctgaag
     1321 taagtctcca tattcacggc aaagtcgcct ttgcatcggt ttatcagttg gccgtctata
     1381 tccatttcat gccatggaac ctcgtctaat aacaccggag gcctgccgag ctttaaatcg
     1441 ttgttgtccc tcatgcgttc tttattcacc cggtggatat caagagccgc aacttcggcg
     1501 tattccttag ccagttcccg aaggatccct atttcataac tttcaatctt cattctaccc
     1561 agcccctttt aacatataaa ataaaatcca tcaaattcat atgctataac gggtgcataa
     1621 tctaaataaa aaagacgcac ccccataatt taaactgcgg accttatgcc ccatttatgc
     1681 aaacgctaaa acccatcaat ccctctctag gatcccctgc tctatagaat cctccaccat
     1741 atcctgaacc aactcccata ggctgggggc accttccttt ataggcaaat tgcgttctat
     1801 caccttactg ctcttaatac cgtatttcac ccatgtatgt ccgccggtat gataattaca
     1861 tataagaggt tgcagcctat caagggaaga cgcaaagaga gcctccgggg tctccttttc
     1921 ctcaaactca tcccatagag ctcttagttc ctgtccctgg tccgagggta ggaggctgta
     1981 gagcctttcc gctgccacca attccctatg ggctttatcc aagttcgcct tttcatcata
     2041 gcaaaaggta tctcccgcat agatttctac caaatcgtgt accaataaca tctttaagac
     2101 tttagttata tccaaatcct ttttaggtga atgttccttc agcaccatcg ccattagggc
     2161 tagatgccag gagtgttcgg catcgttttc gttcctcgac ccgtccatca acagtgtctg
     2221 ccggtagatg tttttcagtt tatccacttc tacaataaaa tcaatttgct tttttaatct
     2281 ttcgctgtcc atccaatatg acatctcctt ataacaagcg gtttaaaaca aaccgtttaa
     2341 aaatccataa ttcgttaata catatagaac aacgcctata acagctatgg taagcacaaa
     2401 cttaagtatc tttgctgcga tcttcattat caacacaatt gccagtatcg caataactat
     2461 agtgataaaa tctgccattt ttatccctcc aaatattgtt ttagcctaga tgatgatata
     2521 ggctaggact catcataact atttgcgcaa tatacaatca tgccagctct aataaataat
     2581 gctatgataa attacgaaaa tctgatacca ttatatcatg agtgcaagcc ttaattggct
     2641 tgcttcacat caattcaata aatgcatcag agaaaaccct aaaggtttta ttaccttgat
     2701 tgcatttatt atatcatgca aacggctccg gattcaattg taggattgcc agagcccccg
     2761 aagaattcat tgttataggg tacagatatt tatgtgtttt tctttgaaag tgctataatt
     2821 aagattcgta tgcaatatcc gactatggag ggattgatta aagtgttttc aaatatttgt
     2881 ctaggagacc atagacccct ttatctacag ataagagatt atataaagcg aatgatctta
     2941 aaggggttgc ttggccaagg ccaaaaacta ccctccacca gagagctttg cgacaccctc
     3001 ggcgttagcc gtaacaccgt tattttggcc tatgaatatc tggaggatga aggttttgtc
     3061 caaattgtca ggggcagggg aacctttgtg tcagatatca acatcgatta tcaaagcgta
     3121 tggagtatgg attggactcc caggttgaca ccctatgccg acctagccaa cgagctggat
     3181 atcgtcaaac atgaagctaa gtggcaaaag ggcatgatat cctttaagag cattgccccg
     3241 gacgacaatt tgtttgatct agaggatttt aaaaaagctt ttttgaaccg attttccctg
     3301 gaaggagaaa agctattaaa ctatggctac gctcaggggt acaaacctct catagattat
     3361 ctaacgcatt atatggagaa taaaggggtc aatatccaag gcaaaagcat tctaataact
     3421 aacgggttta ccgaagggtt tgatatagca ctctccgccc ttactaggcc gggagaccat
     3481 atactatgtg aaaatccaac ccataacaca gccataaaga ttatgaagct ccatggactg
     3541 aaaattgtag gcgtggatat aaacgaggat ggtatagacc tcaatatact aaaagaagcc
     3601 ttatccaata attctatatc tatgggtttt tttattccat cctaccacaa ccccacggga
     3661 atagtcatgt cccccgagaa aagggtcgag gtgctcaaaa tttttggtcg ctatagggtc
     3721 cccattatag aggatggttt caacgaagaa ttaagatatt cgggctctca cctatcccca
     3781 ttagctgccc tatgcggtag gggtaatagt ataatctata taggaagcct atctaaggtt
     3841 ttgtttccag gccttcggat tgggtggatc ttgggggata gcgaattgat ctctcggctg
     3901 gagagcatta aacggagtcg aaacatacat tcttcatttc tggatcaagc tatcctctat
     3961 gaatatctgg ctgcaggaaa ccttgaaaag tatctaaaaa aggcgcgcag gatctaccgt
     4021 gaaaagtatg attatgccct ggaatgtgcc aaaaggtata taccctgtca gagggttctt
     4081 ggggaaggcg gccttcacat attcattgaa cttgagggta acctcaatgc ccgaaaggtt
     4141 ctggacaaat gcatccggga aaatgtcata tttatgccgg gggatatctt ttatatccat
     4201 ggaaaaggtg ctaatacctt tcgtttaggg tttgcccgct cctctttgga ggaaatagaa
     4261 aagggcttta aaatcatagg caatacggtt aggggcctta gctaggcccc taactttgta
     4321 aatcccatat tcgtatcttt tttgcctcta gaaaatcctt taattacatg gtgtagctgg
     4381 cagcgtttat aatctttgta tccttatctc ccatgcctaa tcctcccatt aagcccttta
     4441 acgtatccag tttacctttt tgactgtctg taatcattcc tacacccttt gaaacggttt
     4501 ctacggcgct tttttgatcc tcgctacaat ttataacacc gcagtctacg aattctagtt
     4561 tctcttctat ctcatccgag tctatgtcgt cttttatatt tacaataccg caatcgaaaa
     4621 ctctaatccc atccgcgtat ttatccagtt ttctcttatc tattgtcaaa aaggccctat
     4681 cccccatgcg atgccctttg gtttcttcta tggcgttata ttctacatct atatcatgaa
     4741 atttatccac aagcctgcta cttataaata ctgttccatc tatttttgcc tttgtcaaat
     4801 catctaaagc agcttcgctt tccggcccta tagtcaaatc tccgtctaca tatagcctat
     4861 ccccgtgttt tcttattaaa acattattta gcacttgacc ttctgtatag acaagcccat
     4921 caggtatagt ctttatctct gcatgctcat cgaaaagggg taaggcgcct tccaataggc
     4981 tttccactat aatagcctct tttgtgataa atttcgttcc catctcattt aaagaagcca
     5041 catttaaatc cttatccgac ataacaactt tattccttac ataatacttc tcgccctttg
     5101 cccttaagat aaaggtctta tctataacta attcgcttct gagcctaatg gcatccccgg
     5161 gataggaatc tgtactgcca tttactttaa ttatcggcag cctatccttt atattgtcgg
     5221 gataagagac cagaccattt acttgaatat gagtaaactt gtctagcacc tcttgggatc
     5281 tcgtttctat atttagactt ccgttcacaa ttaatattac aggttttgat aacagggtac
     5341 tatctttaat ctcataagat ccgttctgaa ccattatctc aacatccttg gaaacctccg
     5401 tcacctcagc ggtattcata gctacattgt gctttgccaa aagatcctta gtctcttggg
     5461 acactaaaac catggccgca tttatgctta tatccttgta ggattcaagc accgtctccg
     5521 aaacatttct cgcatcacag actgcagcat taattactaa atttttcttt tccacaacaa
     5581 tcacctttcc ctctctctat aatatatttc agacattttc cttctagccg aagatagcct
     5641 tgacctaaca gttgaggggg ataaattgaa tagttcaccc agttcccttg aggtataccc
     5701 ttctatatac ctcatccaaa ataatgtcct ttctttatcc ggcaagccag cgcacaactg
     5761 acttacaata attttgctta aatcatcttc tttaaacaga ggttcttcta tcttgggcgc
     5821 atttgctaca cgtcttaccc tgtcaataaa gatatttttt gcggccctgt atagccatga
     5881 ccggcattgc gcatctgtta aatcattaag aatatgggta ttctccaagg ctttcataaa
     5941 ggtttcctgt acgatatcct cagcgattga agggttgttt cgggagaggt ttaagcagta
     6001 ccacagaagt ttattgcgat agttcttgta taatttattg atcaaattac ccctcctttt
     6061 gaaatgtctc tacttataag acgaatggaa agtgataagt gttgatttta ttttcatcta
     6121 ctgagaccac tacacttctg tctggtcaaa ttctaagatt aatattttat accttgattc
     6181 tgcgaaaaat atataatata atatgttttg gttcgcttag tagattgggg ggattgattt
     6241 gtctgagttt tttgaattaa atcagaaaat tgagattctg cggggtaagt taaatgagct
     6301 tttatcagat aaaaacccgg taagtaaaga ccggatcatc cgatatagcc gtgaattgga
     6361 cgaacttatc gcaaaatatc atgccatcga aaatatgcaa ggacctacgg aaccactata
     6421 acttttttat caccccttct ttaaaaactc ctcttttgtt ttaacatgtt ccagatattc
     6481 atcttcagta agaataccac tatcgtatgc atcttttagt acatttataa atttaacgca
     6541 gagtcccttc aatttttcct ctatttgata tgtatttgat aaatgtttac tcgctgccaa
     6601 ataatctacc tccaaatact agtttcaatc tgattgttaa ttgtcaaaca attgatcgcg
     6661 aatatttagg gtgataatcc gtgtccccct gtccttgtaa cgcattattg tcgatccccc
     6721 caaaacaagt ttgtatagaa ccggcgaatt tgaataaagc ctaactatgg tgctttcaag
     6781 ggagaaacca ttaggtagtt attctatact attctataca aaatcccata ttcctccacg
     6841 tattaatata taatcatcaa attagtatta atttcctaca tactttgctt tcctactgta
     6901 ccctgtttcg gccaagcctt ttggctttgt agagccgctt atccaccaac ccaacgacat
     6961 cttcaactgt catatcctcg gcaccgccat aaaccatata cacgccgaag ctacaagtta
     7021 aactgatggc ccctccatct atgatgaatt gcttcttctc tataccacgc ctcatccttt
     7081 ccgccacttt ctttgcaccc ttggtatctg tgttggatag acatataaga aattcttcgc
     7141 ccccgtatct tgccacccaa tccttaccct ctcttatgta ccctttcaat tgtccggcaa
     7201 attccttcaa tatctcatcc cctatggcat gaccataggt atcatttatg ttcttaaaaa
     7261 aatctatatc agcgagaata atcgataatg gttctcccct tgtggaagcg ttcagaaggt
     7321 ccaccggtaa tctttcgttg atatacctgc gattataaag atccgtcaag ggatccctta
     7381 tggcaatgcg gttcatatat tcaatcatag tataagcctc tgacccttgg ctttccttat
     7441 ctatgcccat gattaaactc ttggtggctt ctttgagaag ctctaccact agtcttttac
     7501 catggatgat cactgggatg ccggtaaaca tatatatatg accctcctta tgctctaacc
     7561 tataaatagc atcatcttcg ttaaatgctc ttatagaaat acagttttcg cataactccc
     7621 gttttttcca aagcttgtag caaggaatat ctgcatcaaa aaattggtct ccctttaact
     7681 caagcacttt ttttctaatt ggatctacta ttcgcattac atcatataat ttttcgaaaa
     7741 tatgaagttt atcgagaaca atatctaatt cttgcattat gtttcctcca gataaaaccc
     7801 ttgaattcta ctctattata acataattag caggatattt atcttttttc gacaatttac
     7861 atcatttgtc agcaatatct gaccagtgcc ataattaata gggagcaaac aggattcctt
     7921 tgtacgcctt tatgtgaaaa atacgtcatc tagctatcct ctcctcttga aacaaggata
     7981 gagtaggtgt atgatgatta cccagaaggt attgtggtga taatcataca atttatttat
     8041 catgggcctt ttggtctatg agcatttagg ataacctata gaggcagagt aactaaagaa
     8101 ttgaggggaa aatatggaac cagtatctaa aacacccgat ggccaaactt tgatagaaac
     8161 ccataggcat tgtaaattta ttgaagcctc tgtgtccctt agcgaaaaaa caattagaag
     8221 atgggatcct tatttctgta acctcaagtt tgttctaata tttcttgtga tattaggcca
     8281 tttaatagaa aaacacatat atgttaacca ctatttgtat ataatataca cggttattt
//
LOCUS       DASRNX010000150         5803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_507393,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000150 DASRNX010000000
VERSION     DASRNX010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507393"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            542..1108
                     /locus_tag="VFD89_06130"
     CDS             542..1108
                     /locus_tag="VFD89_06130"
                     /inference="COORDINATES: protein motif:HMM:NF016216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HZJ57805.1"
                     /translation="MTHIYDILESNPIIGALPQMVLPNEKEQEKVEVFFLLSGSVMEI
                     EKRVAFLKGLGKKVFIHADLIEGLAKDIIAIDYIKTHIKPDGIMSTRSNLLRHGKEIG
                     LITVQRIFVIDSLSFESGIKMIENYRPDFVEVMPGIVPSAISVLKGKISPPIIAGGMI
                     KRKSDVIQALKAGAMAISTSKRELWDLD"
     gene            1164..2660
                     /gene="glpK"
                     /locus_tag="VFD89_06135"
     CDS             1164..2660
                     /gene="glpK"
                     /locus_tag="VFD89_06135"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZJ57806.1"
                     /translation="MGEKYVVALDQGTTSCRTIIFDRLGRAVTQVSKEFSQIYPEPGW
                     VEHDAVQIWECQLSTLKDALKSKNISPDDIASIGITNQRETLVVWNPKTGKPIYNAIV
                     WQCRRTAGICDELKQKGMEDIIYKKTGLVIDAYFTATKLYWILNQVPGARAMAEDGEL
                     LAGTIDTWLMWNLTGGKVHATDYSNASRTMLFNINELCWDDDLLETLEIPFRMLPEVK
                     DSSGYFGSLDKELLGREIPITGVAGDQHAALFGQACFEKGMVKNTYGTGCFILMNTGD
                     ERKFSANNLLTTIAWRIGGRTEYALEGSVFSAGAAVQWLRDEMGIIETAPETEALALA
                     VDDTNGVYVVPAFVGLGAPYWDPYARGTILGLTRGANRKHLVRAVLESIAYQSTDVLK
                     VMEKDSGIDIIQLRVDGGASANNFLMQFQADISGLPVVRPENTETTARGAAFLAGLEV
                     GVWKGKKDIEAIWKEERKFDPKMEQETRDEKYRLWQKAVSRSTGWIEP"
     gene            complement(2664..3563)
                     /locus_tag="VFD89_06140"
     CDS             complement(2664..3563)
                     /locus_tag="VFD89_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57807.1"
                     /translation="MIKFTKNKGFVAVLLIALVLLAGVGCDKVFRRPKEKQSKQDQEQ
                     KKSPPKTLTSLENTTETMIKSIQEVEEKRAEAAQQKQGKEQESGQDKGKQEEEKKEEK
                     PEDKDKKQEEKLEEGGQQEKSKEKQEKPEPLDWDRMQKNVEKLQKSWSDYVNTALKDG
                     ASTDLVKEYENQLDTLTTKVMEEQEESLLDTANDLYKYYPKFFDLYKHKAPPNIKDMK
                     YYIRKIIIAGEKSQWTDTQKSMDAIKQAWDIAKSRMEKPDKDLNRRVETAIDSFSRSV
                     KQENTHLVKLKGNILMDMLEEIK"
     gene            4106..5764
                     /locus_tag="VFD89_06145"
     CDS             4106..5764
                     /locus_tag="VFD89_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HZJ57808.1"
                     /translation="MPEQEIIKVRLGDKTLEVERGTVIEELLSDHGAGKSTQVVAAMV
                     DNKIQELTQTLDRDCRVDFVDLTSKDGIRIYVRGLTFIFIRACRELFPECKVTIEHSM
                     SKGLYCEVHGNFTLTPSKVKRVEERMREIVKLDEPFERNIVSIDEAKGIFSKMGFDDK
                     LEILEYRREEDIRLYSCGWMTDYLYGYMVPRTGYLKAFELLFYLPGVILRHPTQETDG
                     KIPEFVDSPNLYRVFRDSEKWGQSLNVENTAQLNRTIKEGRGGDLIRVCEAHLENQIA
                     RIAEEINSHADSIRLILIAGPSSSGKTTFAQRLRVQLMVHEIDPIPISMDDYFYNRED
                     IPVDENGERDLESIDIIDIELFNEQITSMIQGEEVELPRYNFQKGQREYRGNKVKLTS
                     DQLIIVEGIHALNEKLTAMVPQSNKYKIYISALTQLNLDNHNRIPTTDTRLIRRMIRD
                     HQFRGASIEETLNMWGNVRKGEEKNIFPFQEQADVMVNTSLVYELAALKPYILPLLEE
                     FDEDNPYYIEVNRLKAFMKYFVELKAKEIPNNSILREFIGGSCF"
BASE COUNT         1559 a         1197 c         1525 g         1522 t
ORIGIN      
        1 ggatatgaat atatcatttg catgggccgg gaaatgattg gggtaattgg ggatgcatgg
       61 gccactaggc ataaaccgac attttttcag ccccacagac accgtttttt attggcaggc
      121 gcacccttta tatttgaaga attatatgga aatcacatag tgtaaaggct tagaacctgt
      181 cgtgcagcgg aggataggcc gaatcattgt ttgggcgtag cgagttcgag caggccctgc
      241 agcaagctta cagattcttg gccttgaaac tttagtggtt ggaatattat tctttaattt
      301 agattatgca ccctttattg acgcatcaat tgaagccagt gactttagtg tggtataatg
      361 tatatggttt cacggcgggt gataccctta tacacgtttt tgatacttat cttcagataa
      421 gcaatgggtc aactaaattt atttacatga taattgcata ggctttatgc aatctcacga
      481 tgataagttt tgtagctgga attgtttgtc tatatttggg atcttatggg ggggaagagt
      541 tttgacccat atatacgata tactggaatc caaccctatt attggagcac tgccacagat
      601 ggtattgccc aacgagaagg aacaggaaaa ggtagaggta ttttttttgt tatccggcag
      661 cgtaatggaa atagaaaaaa gggtagcttt tttaaagggg ctggggaaaa aggtttttat
      721 tcatgcggac cttatcgaag ggctggcaaa ggatataatc gccatagatt atataaagac
      781 ccatattaaa ccggatggga ttatgtcaac tcgcagcaat ttgttgcgac atgggaagga
      841 aatcgggctc attacggtac agcgtatctt tgtaatcgat tccctgtcct ttgaaagcgg
      901 gataaagatg atagaaaatt ataggccgga ttttgtggag gtgatgcccg gaatagttcc
      961 cagcgctata tccgtgctta aggggaagat atcgccgccc attatagccg gcgggatgat
     1021 taagagaaag agcgatgtga tacaggctct aaaggcgggt gccatggcaa tttccaccag
     1081 caagagagaa ttgtgggact tggattagag taccgccgag agaaactgaa ataaatgcat
     1141 ttattgaaag aggggcgagg taaatggggg aaaaatatgt agttgcattg gatcagggga
     1201 ccaccagctg caggaccatt atttttgaca ggctgggaag ggcagtgact caggtatcca
     1261 aggaatttag ccagatatat ccggaacccg ggtgggtgga gcatgatgct gtacagatat
     1321 gggaatgcca gctgtctacg ctaaaggatg cactgaagag caaaaacata tcgccggacg
     1381 atatagcatc catagggata accaaccagc gcgagaccct ggttgtatgg aacccaaaaa
     1441 ccggaaaacc catctataac gctatcgtat ggcagtgcag gcgaaccgcc ggcatctgcg
     1501 atgagctgaa gcaaaagggc atggaggata taatctacaa aaaaaccggt ttggtaattg
     1561 atgcatactt tacggctaca aagctgtatt ggattttgaa ccaggtacct ggggccaggg
     1621 ccatggcaga ggacggagaa ctgttggcgg gaaccattga cacctggctt atgtggaact
     1681 taacgggcgg gaaggtccat gccactgatt attcaaacgc atccaggacc atgctcttta
     1741 acataaacga gctgtgctgg gacgatgacc tgctggagac cctggaaatc ccctttagga
     1801 tgctgccgga agtgaaggac agcagcggat attttgggag cctggacaaa gaactgttgg
     1861 gccgtgagat tcccataacg ggagtggctg gggatcagca cgccgctcta tttgggcagg
     1921 cctgctttga aaagggaatg gttaagaaca cctacggtac cggatgtttc atcctgatga
     1981 acacggggga cgagagaaaa ttctccgcca acaatctcct tacaaccata gcctggcgaa
     2041 tagggggcag gacagaatat gcgctggagg gcagcgtgtt ctccgccggc gcggctgttc
     2101 agtggctcag ggatgagatg gggattatag agactgcccc ggagaccgag gctctggctc
     2161 tggccgtcga cgataccaac ggggtttatg tggttccggc ctttgtaggg ttgggtgccc
     2221 cttattggga tccctacgcc aggggcacga tattgggtct gacccggggt gccaacagaa
     2281 agcatttggt aagggccgta ttggaatcca tagcctatca atccacggat gttttaaagg
     2341 tcatggaaaa ggattccggg atagacataa tccagttgag ggtggacggc ggcgcatcgg
     2401 ccaataactt cctgatgcaa tttcaggcgg atatatcagg ccttccggtg gtaaggcccg
     2461 aaaataccga gaccacggcc cggggtgcag cctttttagc cggccttgag gttggggtat
     2521 ggaagggtaa aaaggatatc gaagccatat ggaaagagga acgaaagttt gaccccaaaa
     2581 tggagcagga gacgagggac gaaaagtata ggctgtggca gaaggccgta tccaggtcta
     2641 ccggctggat agagccttga agcctatttt atctcctcca gcatatccat cagtatattg
     2701 ccctttagct taaccaggtg ggtgttttcc tgttttacgg atctggagaa actgtcgatg
     2761 gcggtttcta ccctgcggtt taaatcctta tccggctttt ccattcggga ttttgcgata
     2821 tcccaggcct gtttgatggc atccatggat ttttgggtat ctgtccattg acttttctct
     2881 cctgctataa ttatttttcg gatatagtat ttcatatcct ttatattggg cggcgccttg
     2941 tgcttataca ggtcaaagaa ttttgggtag tacttatata ggtcgttggc ggtatctaaa
     3001 agcgattcct cctgctcctc catgaccttg gtggtcaagg tatccaactg gttttcgtat
     3061 tcctttacaa gatcggtgga ggcgccgtcc ttgagggccg tgttgacgta gtcactccac
     3121 gacttttgga gcttctccac gtttttttgc atcctgtccc agtccagggg ctccggcttt
     3181 tcttgttttt ctttggactt ctcttgttga ccgccttctt cgagcttttc ctcctgcttt
     3241 ttatccttat cctcgggttt ttcctctttc ttttcttcct cctgttttcc cttatcctgg
     3301 ccgctctcct gctccttgcc ttgcttttgc tgggctgcct cggcacgctt ttcctctacc
     3361 tcttggatgc tcttaatcat ggtctctgtt gtgttttcaa gggaggtaag agtctttggc
     3421 ggggatttct tttgctcctg atcttgcttt gactgctttt ccttaggacg cctaaacacc
     3481 ttatcgcatc ccacacctgc aagcagcacc aatgcaatca gcaaaacggc tacaaatccc
     3541 ttgttcttag tgaatttaat cataattaca cacctcaaaa attcatatgg tctagggatt
     3601 agtatttcac tcggcgcctc ataaataccc aatatttatt tttaagagat aaagcccgta
     3661 tgctgccgat tggatcaatc atctcggagg ggactttata ggccgtccta aaaataagcg
     3721 gttgtatttt ttatccccta gattgtttga taataatggc tgggtgttag tataatatag
     3781 acaaacaact ccaaccgcca aatttatcat ctgcagataa atttagttga cctcttgttt
     3841 atctacggtt gtttatctat ggacgttgga aagggattac ccgccaaaat ctggatttgt
     3901 gccttggagg tcggggactt gaagttaaaa tgctacatag aaaaaagcgt aggccctccg
     3961 gaccggtggg tttggccttg gtaatatcgt tagggtgcta cataacaaaa agcgtaggta
     4021 cccttcctag ggatttgcac aaggatatgg acgatttaaa aatcataaga attagagaaa
     4081 ttcgattata ggagtgaaga aacgtatgcc ggagcaagaa attataaagg ttaggttagg
     4141 ggacaagacc cttgaggttg agagggggac ggtcatagag gagctactat cggatcatgg
     4201 cgccggaaaa tcaacgcagg tggtggcggc catggtggac aacaaaatac aggagctgac
     4261 ccagaccctg gacagggact gcagggttga ttttgtggat cttacatcca aagacgggat
     4321 taggatatat gtaaggggcc tgacctttat atttatccgc gcctgtcgcg aactgtttcc
     4381 cgaatgcaag gtgaccatcg agcattccat gagcaaaggg ctctactgcg aagtgcacgg
     4441 taactttacc ctaaccccca gcaaggttaa aagggtagag gagcggatga gggagatagt
     4501 aaagttagat gaaccctttg agcgcaatat agtttcaata gatgaggcta agggcatctt
     4561 cagtaaaatg gggtttgacg acaaattgga gattctagaa taccgccggg aagaggatat
     4621 acgcctctac agttgcggtt ggatgacgga ttacctgtat gggtatatgg ttccgaggac
     4681 cggttatcta aaggcctttg aactcctgtt ttatttgccc ggagttatac tcaggcatcc
     4741 aacccaggag accgacggga agataccgga gtttgtagac agcccaaacc tatacagggt
     4801 tttcagggat tccgaaaagt ggggtcaaag ccttaatgta gagaacaccg cacaattgaa
     4861 taggaccatc aaggaaggca ggggcgggga tctaatcagg gtctgcgagg ctcatttgga
     4921 aaatcagatt gcgaggatag cggaggaaat caattcccat gccgacagca ttaggcttat
     4981 cctgatagcc gggccgtcgt cgtcgggcaa gaccaccttt gcccaaaggc tccgggtgca
     5041 attgatggtc catgagatag atcccatacc catatccatg gacgattact tttataacag
     5101 ggaggatatt cctgtggatg agaatggaga acgggactta gaatccatcg acatcataga
     5161 tatagagctc ttcaacgagc agataacctc catgatccag ggtgaggagg tggagctgcc
     5221 cagatataac ttccaaaaag ggcaacggga atataggggc aataaggtaa agctgacgtc
     5281 ggatcagctg attatagtcg agggtatcca tgcgctcaac gaaaagctga cggcaatggt
     5341 cccccaaagt aataagtaca agatatatat cagcgcattg acccagctaa acctcgataa
     5401 ccataacagg atacccacca ccgatacccg gcttataagg aggatgatca gggatcatca
     5461 atttaggggc gcatccatag aggagacctt gaatatgtgg ggtaatgtac gaaaaggtga
     5521 ggagaagaat atatttccct ttcaggagca ggcggatgtc atggtaaata catccctagt
     5581 ctatgagctg gcggctctaa aaccctatat cctgcccttg cttgaggaat tcgatgagga
     5641 taacccctac tatatagagg tcaatcgctt gaaagccttt atgaaatatt ttgtcgagct
     5701 aaaggccaag gagattccca acaattcaat cctcagagaa ttcataggag gaagctgttt
     5761 ttagatacgg gcataaaaag agcaacccgg gtgggttgct ctt
//
LOCUS       DASRNX010000151         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_717143,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000151 DASRNX010000000
VERSION     DASRNX010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717143"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..2084)
                     /gene="brxL"
                     /locus_tag="VFD89_06150"
     CDS             complement(36..2084)
                     /gene="brxL"
                     /locus_tag="VFD89_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HZJ57809.1"
                     /translation="MPDLLEREIVTDKLFKHFAGKIVRKDLTKGIKEGANVPVYVLEY
                     LLGMYCSSQDEVAIEQGVQNVKEILARNYVRPDEAQKIISKLREIGSYTVIDKVSVKL
                     NYRTDIYEAEFSNLGLRGVPIPGVYPSDYERLLAGGIWCIVQMEYFYDEGDRNRNPFI
                     INKLTPVQMPGIDMDEFKRSRGEFSTQEWIDVVLRSTGMEPDKLTEREKWLHLIRLVP
                     LVENNYNFCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRQVGLVGMWD
                     CVAFDEVAGITFKDKDGVQIMKDYMASGSFARGKEEKNAMASFVFVGNINHSVDVLLR
                     TSHLFEPFPEAMGMDTAFLDRMHSYSPGWEIPKYMPDFFTDDYGFITDYLAEVMRELR
                     KTTYADSYEKYFRLGNQLNQRDSIAVKKTVSGMIKLLYPHGEYTKDDVEEILRFAMEM
                     RRRVKEQLKKIGGMEFYDVNFSYIDNDTFQEEYVPVPEQGGGKLIPEGIGKPGHIYSI
                     GRGSSGMLGVYKLETQMTSGSGKFERTGIGANPRARESVDNAYKYLRANSTKISGTIS
                     TTTKDYLIHIQDLNGVGMTTSLTLPTIIAISSVALGKPLVTSAAVLGDVSIGGTLIKV
                     EELANTLQVCQDSGAKKVLLPLTSAGDLGVVPPELVGSFNIIFYKSAEDAVFKALGVE
                     "
BASE COUNT          536 a          583 c          379 g          637 t
ORIGIN      
        1 cctttatatt attctgcata tatcccctta ttcctttatt ccacccccag cgccttaaac
       61 accgcatcct ccgcggattt ataaaatata atgttaaatg aacctacaag ttccgggggc
      121 accactccca aatctccggc ggaagttaag ggcaacaaga ccttttttgc cccactatcc
      181 tgacacacct gaagggtatt ggccaattcc tctaccttta ttagggtacc ccctatactc
      241 acatccccca aaacagccgc gctggtaaca agaggtttgc ccagggctac cgacgatatg
      301 gctattatag ttggtagggt gaggctagtg gtcatgccta caccgtttag atcctgtata
      361 tgtataagat aatcctttgt agttgtgcta atggttccgc ttatcttggt actattagcc
      421 cttaggtatt tataggcgtt atccacagac tccctagccc tgggattggc tcctatacct
      481 gtccgttcga acttgccact tccggaagtc atctgggttt ccagcttata aacccccagc
      541 atcccggacg aaccccttcc tatgctgtat atgtgcccgg gctttcctat gccttcgggg
      601 attagctttc caccaccttg ttccggtacc ggtacatact cttcctgaaa ggtatcatta
      661 tctatatagg aaaagtttac atcataaaat tccataccgc cgatcttctt aagctgctcc
      721 tttacccttc ttcgcatctc catggcaaac cttaggattt cctctacatc atcttttgta
      781 tactccccat ggggatatag aagttttatc attcccgata ccgtcttttt tacggcgatg
      841 ctgtcccttt ggtttagctg atttcccagc ctaaaatact tttcataaga atcagcgtaa
      901 gtagtttttc taagctctct cataacctct gctaaataat ccgttataaa gccatagtca
      961 tcggtaaaaa agtctggcat atactttggg atctcccatc cgggagagta agagtgcatc
     1021 ctatctaaaa atgccgtatc catccccatg gcttccggaa agggttcaaa gagatgggat
     1081 gttctaagca gtacatcaac cgaatgattt atatttccca caaagacaaa ggaggccatg
     1141 gcgttctttt cttccttgcc cctggcaaag gaaccggatg ccatataatc cttcattatc
     1201 tggactccgt ccttgtcctt aaaggtgata cccgccacct cgtcaaaggc tacgcaatcc
     1261 cacatcccaa ctaaacccac ctgcctggag gccatgttgt aaaacaggtt tgcaacggtg
     1321 gtttgacccc cggaaaccaa gatggaatta ggggatatct ccttatatac atgagatttg
     1381 cccgtgcccc tgggcccaag ttcacaaaaa ttataattgt tttctaccaa gggtaccaat
     1441 ctaattagat gcagccattt ttcccgctcc gtcagcttat caggttccat tccagtgctt
     1501 ctaagcacta catctatcca ttcttgggta gaaaactctc ccctactcct tttaaattca
     1561 tccatatcta ttcccggcat ctgtaccgga gtcagcttat ttatgataaa ggggttgcga
     1621 tttctatcac cctcatcgta gaagtattcc atttgtacaa tgcaccatat ccccccggcc
     1681 aatagccgct cataatccga tggatagacc cccgggatgg gcacacccct taagcctagg
     1741 ttggaaaatt ccgcctcata gatatccgtc ctatagttca gtttgacgga aaccttgtca
     1801 attacggtat agctccctat ttcccggagc tttgatatga tcttttgggc ctcgtctggt
     1861 cttacataat tccttgccag aatctcctta acattttgca ctccctgttc tatggcaacc
     1921 tcatcctggg atgaacaata catcccaaga agatattcca gaacatacac ggggacattg
     1981 gctccttcct ttatcccctt ggttagatcc tttctcacga tcttcccagc gaaatgctta
     2041 aacagcttat ctgttactat ctccctttct aataagtccg gcactaaaat ccccccttat
     2101 atatcaaatc caaaatcgtc tgcaaaggct atatc
//
LOCUS       DASRNX010000152         4166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_296805,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000152 DASRNX010000000
VERSION     DASRNX010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296805"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFD89_06155"
     CDS             <1..631
                     /locus_tag="VFD89_06155"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HZJ57810.1"
                     /translation="TNTKSNSPKGTILYQDKSNTVIGIRTTINITVSQGKVIYVPDFV
                     APKGSGYDAAITREKAIAICEELNIIPVFKEASKQGRLPGEVWSQSIEAGKEITEGST
                     ITLKYVPANVKVTVPDFKGKTEDQVLAGGHNKNFDIRFEVGMEFVEGFEGKVVSQSPK
                     ASSKTAAGSTITLTLGPEIPDGPDPSGNSGDSDPGLSGGSGGSETPDSD"
     gene            835..1137
                     /locus_tag="VFD89_06160"
     CDS             835..1137
                     /locus_tag="VFD89_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57811.1"
                     /translation="MDNKILKQIKDLRRELRDVNITDIPQLQDISNTTRAVDFWFDNI
                     FADLNVRNKIRGNKEQTIKLMGSINGIIRRLERNRAGIHGEIERLEQDKIDLIIRD"
     gene            1151..1888
                     /locus_tag="VFD89_06165"
     CDS             1151..1888
                     /locus_tag="VFD89_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57812.1"
                     /translation="MDILKVVYSKTVIDSKRKRISFLLKERSTNIKSGVIEIISTGDL
                     GLLFGLYDEIFFDNWFRDSFKGKIKFSLSRRMTKSAGKTLCPKDIDRIDEKELVMEIR
                     IGTDFFFNYGELKGNKSVCGIQTNTALEALQLVFEHEICHVLEFINFKESSCSGDRFK
                     TLANNLFGHMESYHKLPTNRQIANQKYGFKIGDRVSFVFDGRQLSGILYNITKRATVM
                     VEDDNGIMADDRGNRYTKYYVPLPLLK"
     gene            2063..2252
                     /gene="ssrS"
                     /locus_tag="VFD89_06170"
     ncRNA           2063..2252
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFD89_06170"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2242..2382
                     /locus_tag="VFD89_06175"
     CDS             2242..2382
                     /locus_tag="VFD89_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57813.1"
                     /translation="MDYLLDDISHRCIWDASMAFLTAAYSPLYKFIGRSILAKNSKKL
                     SC"
     gene            2826..3407
                     /gene="rpsD"
                     /locus_tag="VFD89_06180"
     CDS             2826..3407
                     /gene="rpsD"
                     /locus_tag="VFD89_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZJ57814.1"
                     /translation="MAKPKKPRFKVARHLGENVYNHPKALKRGVKSRRKQSEYGKQLL
                     EKQKLKEYYGLLERQFRRNVREALRSKENPGVVLVQNLERRLDNIVYRLGYGSTLRQA
                     RQIVGHGHIRVNDQKVDIPSLRLNPDDRISLNERSRNKDLFRDNFQAAPLTLSYLEKD
                     EETLTGRLVSIPDIDEIPINVDTTPIIEFYSRS"
BASE COUNT         1324 a          749 c          990 g         1103 t
ORIGIN      
        1 gaccaatacc aagagtaatt cccccaaggg gaccatcctc tatcaggata agagcaatac
       61 ggtcatcgga ataaggacca ctataaatat aaccgtctct caggggaagg tcatatacgt
      121 accggatttt gtcgctccca aaggttccgg atacgatgcg gcaatcacca gggaaaaggc
      181 catagccata tgcgaggaac tgaacatcat acccgtgttt aaggaagcca gtaaacaggg
      241 aaggctgccg ggcgaggtat ggtcccagag catcgaggcg ggcaaggaga taacggaggg
      301 gtctaccatt accctcaagt atgttcccgc caacgtaaag gttacggtac cggactttaa
      361 agggaagacg gaggaccagg ttctggcagg gggccacaat aagaactttg atatcaggtt
      421 tgaggtgggc atggaatttg tagaaggctt tgagggaaag gttgtcagcc agtccccaaa
      481 ggcatcctca aaaacggctg cgggcagcac cataacctta accttagggc cggagattcc
      541 ggatggtcct gacccgtcgg ggaattcggg cgattctgac cccggactat cgggtgggtc
      601 gggcggatct gaaacaccgg attctgatta atggataaaa acccggataa atgaatatac
      661 cagtaacacc gtcgataaag gcggaacgca cattgcactg tgtatgttct gcctttattg
      721 ttaaatcgat catacacatt ttcgatttta gttgttagtt agtggcctga accttatatg
      781 tgtttatatt ttcagaaatg aaaccgtctg atcatctgta tatggggtgt tttaatggat
      841 aataagatac taaaacaaat taaggatttg cgaagggaac ttagggacgt taatataact
      901 gatatccccc aattgcagga tatcagtaac acaacaaggg ctgtagattt ttggtttgac
      961 aatatatttg cagatctcaa cgttcgaaat aagattaggg gcaacaagga gcagacaatt
     1021 aagctaatgg gctctattaa tggtatcatc agaaggcttg aaaggaatag ggcaggtatt
     1081 catggggaaa tagaaagatt ggagcaagac aagatcgatc ttattattag ggattaggcg
     1141 ggctggaaga atggatatac taaaggttgt atacagtaaa actgtaatag acagcaagcg
     1201 aaaaaggata tcgttcctat taaaggaaag gtccaccaat attaaatccg gggtgataga
     1261 gatcatatca acgggtgatc tgggcctgct atttggatta tatgatgaga tattctttga
     1321 taactggttt cgggattcct ttaaaggcaa gatcaaattt tcgctttccc ggcgaatgac
     1381 caaaagtgca ggcaaaaccc tgtgtccaaa ggacatagac agaatagacg aaaaagagct
     1441 ggtaatggaa attaggatcg gaacggattt tttcttcaat tatggggaac taaagggcaa
     1501 taaatccgtt tgcgggatac aaactaacac cgccttggag gcattgcagc tggtcttcga
     1561 acatgagatt tgccatgtat tggagtttat taatttcaaa gaatcaagct gtagcggtga
     1621 tcggtttaag accctggcga acaacctgtt cgggcatatg gagagctacc acaaattgcc
     1681 cacgaaccgg cagatagcta atcagaagta tgggtttaaa ataggtgata gggtttcctt
     1741 tgtttttgat ggcaggcagc tttcaggcat cttgtataac attaccaaga gggccaccgt
     1801 tatggtcgaa gacgataacg gtataatggc ggacgatagg ggaaatagat ataccaaata
     1861 ctatgtaccc ctgccgcttc taaaatagat ccgattcaaa gattttccta ttgaaattgg
     1921 cgttaaaaat ggtataatga taattgaaga aacggcccgg gaaatttcaa agaacacacc
     1981 attcaatacc ttaaggttac aatgggaata atttgggttt cttgaaactt caactgaaca
     2041 gttatttatt tgagtatata tttacaatct gggatattcg tgaagctttt attttgaacc
     2101 tacattgtgc atgagggagt ccaacatgaa gcagtggata tcaagcccat tatgagtccc
     2161 attgcggcgg gggaagatag aaattgtttt gaggacaccc acctatcgag agataggtgt
     2221 caaaatagag gcgacggtat cttggattat ctattagacg atataagcca tagatgcatc
     2281 tgggatgcgt ctatggcttt tttgacggcg gcttactcgc cgttgtataa atttataggg
     2341 cgtagcatct tagccaagaa ttcaaagaag ctgagctgct gaactaatgg tggtattcca
     2401 taggccttaa aataatgccg tatcttttga aacatttctt gttaacagga tctattccct
     2461 gagcaatgtg attcccaaga aaatattcaa atggaaagat atattatgga ttaagtttat
     2521 atgagcctgc gaaacaaaag tgttaaaatc gccgataatg tggtattaat aataacgaaa
     2581 cgggtaaata tgacccgttt cgttgaaacc tgtgtgttgc aattgctaat gcaattgctt
     2641 ccgatgttat aacggggcgg acaaacaacc cccactttta caagtagcgg gtttcgatta
     2701 gttataacaa gcgatggata tggatcgttt cgaatgattt aaagcgatat tttaatcctt
     2761 gggattggcg gttttattcc aagggatttt ggataggcac cttcaaaaaa taaggaggga
     2821 ttgtgttggc aaaaccgaaa aagcccaggt ttaaagtagc gaggcatctg ggcgagaatg
     2881 tatataatca cccaaaggcc ctaaagaggg gtgtaaagtc ccgaagaaaa caatcagaat
     2941 atgggaagca actgctggaa aagcagaagc tcaaggaata ttacggcctt ttagaacgcc
     3001 aattcaggcg taacgtcagg gaggctctga ggtctaagga aaatccgggg gttgtcctgg
     3061 ttcaaaatct ggagagaagg ttggataaca tagtctacag gttagggtac ggatccacct
     3121 tacggcaagc caggcaaatc gtaggacatg gccatataag agtcaacgat cagaaggtgg
     3181 acataccttc ccttcggtta aatcccgacg acaggataag tctcaacgaa agatcaagga
     3241 ataaggatct gtttcgggat aattttcagg cggcgccctt gaccctcagc tatctggaaa
     3301 aggatgaaga gaccctaacc ggacgtttgg taagtatccc cgatatagac gagataccca
     3361 taaacgtgga tacgacacct ataatagagt tttattccag gagttagtag tagataagta
     3421 atacgcaaac taaatttatc ggtatgataa tcgcaacggg tttatgtgat tttatggcga
     3481 tagatttcat agttgtagtt acttatctat gtagataagc aatacggaaa ctaaatttat
     3541 cggaatcggg attctaaccc cgattctttt ctatttctct tgacaaaaag taacatttta
     3601 tttattagat tccccaagaa cactcgtgac tttagtcgtg agatgaattg gggaaaatag
     3661 ccagcataca gattagtctg tatgtagtgg tagctcataa acccccttgt ataattaaca
     3721 atgtattaag cagtgaagga aatcactgca taacttgtaa gtctggcaag ggtaccaatc
     3781 ccttcccaag tagaaatcaa ttctaaatat catacatatt ccggtgccat atcttagtcg
     3841 attttgccag tctttgttgg cctgaacctt gaaagcatgt ataatattat ccggacttgg
     3901 aggcttgatg taaaactccc tgggacagac cataatgggc tgattacccg tgagttagaa
     3961 tattgatcca ttccggtaag tctcacgttt tggctggcaa taccgcttta ccaaaatatt
     4021 gttataatag tcgaaacatg gtataatagc cttatgaaag tgaggtgaaa aaccatgtat
     4081 ttgactgtaa aacagcaagt aaagcagtta tccaaggaag agtataaaaa tatcaaaaag
     4141 ctatgccata tagccaagaa tctggc
//
LOCUS       DASRNX010000153         3954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_401910,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000153 DASRNX010000000
VERSION     DASRNX010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401910"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..2068)
                     /gene="recG"
                     /locus_tag="VFD89_06185"
     CDS             complement(8..2068)
                     /gene="recG"
                     /locus_tag="VFD89_06185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZJ57815.1"
                     /translation="MDLLERSINTIKGIGPKKTELLAKMDIHTLGDALYFFPRDYEVR
                     PEITGMGSVANEEIVSLCGKFKGKPNIIRKHRGFSILEWRAVDKTGEVVCTWFNQPYR
                     TKQYTTDVPYYIYGKAVRAYGKVQIQNPMIEEYMPSRHNCGGLDPIYALVDGLTQKDL
                     RNISQHIIEGIEENIDDDLSPDITGKYGLMDKACALKTIHYPSDSSTLESARRRLIFD
                     ELFFMQLALHGHREARYNAKNGLAFKWDHQTMADFIGKLGFPLTGAQVRVVEEIFRDL
                     KSKTPMNRLIYGDVGSGKTVIAAIALYAAVLDGYQGVLMAPTEILARQHFETLERLFR
                     GMGIRIGLLIGGKTEKEKKRVKELLSADEIQLVVSTHGILQEDVEFSRLGLIITDEQH
                     RFGVRQRTTLVKKGRNTPHVLVMSATPIPRTLALILYGDLDISLIDELPPGRKSIKTY
                     HVPPSMRERIYAFIRKQAKMGYQTYLVCPLVKESQQIHAESAISMYDTLKTGALSNLR
                     LGLIHGRMRSDEKDDVMASFQKGDIDVLISTTVIEVGVNIANANLMVIENAERFGLAT
                     LHQLRGRVGRSKSQSYCILISDLKSDNARERMDVMVKSNDGFEISQRDMELRGPGEFL
                     GVKQHGLPKFKIADLARDIDILNEARIAAEEILKSRDYRYKDTMLKKAYDRFNREMGK
                     VVLN"
     gene            complement(2187..3803)
                     /locus_tag="VFD89_06190"
     CDS             complement(2187..3803)
                     /locus_tag="VFD89_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HZJ57816.1"
                     /translation="MDIEYIDGIKLREMFWAAAQFLEKNKQTLNALNVFPVPDGDTGT
                     NMSLTMVAAIKEMQGTNSNSVAQVADAVSKGSLKGARGNSGVILSQLFRGFSRALQGE
                     TRMSTRECARALQEGAKTAYKAVMKPVEGTMLTVARITGEEAIKLSKKHPDFKTFSNE
                     VIKVAKDTLERTPDMLPPLKEAGVVDAGGMGVLYILMGATQALEGISDMELMPVSEEI
                     FDDMAPEIGTGADIRFTYCTEFFVKNLFPYVTEGDVDKLKERLANLGDSIVVVGDPEL
                     IKVHVHTNMPGKALQLALRFGELSGVKIDNMKEQHRHVIIETKQKEFGIVAVAMGDGI
                     CSIFKDLSVDHVIEGGQTMNPSIEDISKAVERTSAKNVFVLPNNSNIILSAKQAQEIS
                     DSNIVVIPSKSIPQGLAAILAFNPDSDIDTNTTNMTEAMEGVNSGQVTYAVRDSEYNG
                     ITIEKDDIIGLANGKIVTAGKDIAKITTSLLDKMIDDDSEIVTLLYGQDIGQEQAEQI
                     IAYLEESYPDLDIEMHPGGQPLYYYIISVE"
     gene            complement(3831..>3954)
                     /locus_tag="VFD89_06195"
     CDS             complement(3831..>3954)
                     /locus_tag="VFD89_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZJ57817.1"
                     /translation="YGISIAAVAKNIIETVKYNIENLTGMIVKKINVTVEGVRV"
BASE COUNT          987 a          992 c          714 g         1261 t
ORIGIN      
        1 tccctaatta gtttaataca accttaccca tttcccgatt aaatctgtca taggccttct
       61 ttagcattgt atctttatat ctgtagtccc ttgatttcaa tatctcttcg gctgcaattc
      121 ttgcctcgtt taggatatca atatcccttg caaggtcagc aattttaaat tttggcaatc
      181 catgttgctt aactccaaga aactccccgg ggcctctgag ctccatatcc ctttgtgata
      241 tctcaaagcc atcattgctt ttgaccataa catccattct ttccctggca ttatcacttt
      301 ttaaatctga aatgagtata cagtatgatt ggcttttact acggcccacc cttcctctca
      361 gttgatggag tgtcgctaat ccaaaccttt cagcgttttc tataaccatc aaattggcat
      421 tggctatatt tacacccacc tctataaccg tagtggatat tagaacatca atatcaccct
      481 tttggaagga tgccataaca tcatcttttt catcggatct catcctacca tggatcaggc
      541 cgagtctcag gttggaaagg gctcctgttt ttagcgtatc gtacatgctt atcgctgatt
      601 cagcatgaat ttgctgggat tcctttacca atggacaaac cagataggtc tgataaccca
      661 tctttgcctg tttacgaata aaggcataaa tacgttccct catagaaggc ggtacatggt
      721 aagtcttgat ggattttctg cccgggggca gttcatctat cagggaaata tccagatctc
      781 cgtagagaat aagagccagg gtccttggta tgggagttgc tgacattacc aggacatgcg
      841 gggtatttcg tcccttttta acaagagtgg ttctttgcct tacgccaaat ctatgctgct
      901 catcggtaat aatcaaacca agcctactga actccacatc ctcctgcaaa atcccatggg
      961 tggatacaac taattgaatt tcatcggcgg ataataattc tttcactcgt ttcttttctt
     1021 tttctgtttt tccaccgatc agaagaccta tccttatccc cattccccta aaaagtcgtt
     1081 ccaatgtctc aaaatgttgc ctagccagaa tttcggtagg agccattagt accccttgat
     1141 aaccgtccaa gacagccgca tataaggcta tagcggctat aacagttttc ccggatccca
     1201 cgtccccata tataagccga ttcatgggtg tcttgctctt aagatcccta aatatttcct
     1261 ccaccaccct tacctgcgct ccggtcaagg gaaaaccaag ttttcctatg aaatcagcca
     1321 tagtttgatg gtcccattta aaagccaatc catttttggc gttatatcgc gcctcccgat
     1381 gcccatggag ggccaattgc ataaagaata gctcgtcaaa tatcaaacgt ctcctagcac
     1441 tttccagggt actgctgtcc gagggataat gtattgtttt aagtgcgcat gccttgtcca
     1501 tcaggccata ttttcccgtt atatccggcg agagatcatc atctatattc tcttcaatgc
     1561 cctcaatgat atgttggcta atatttctta aatctttctg cgttaacccg tctaccaaag
     1621 cataaatcgg atcaagcccg ccgcaattat gacggctcgg catgtattct tctatcatag
     1681 gattttgtat ctggaccttt ccataagccc ttaccgcttt accatatata tagtatggaa
     1741 cgtctgtggt atattgtttt gttctatagg gctggttaaa ccatgtacat accacctccc
     1801 cggtcttatc taccgcccgc cattccagaa tggaaaagcc cctgtgtttt cttataatat
     1861 tgggctttcc cttaaatttc ccacaaaggg acactatttc ctcgtttgca actgagccca
     1921 tgccggtaat ttccgggcgt acttcataat ctctcgggaa aaaatatagg gcatccccca
     1981 gtgtatggat atccatcttt gccaataatt cagttttctt ggggcctatc cccttaattg
     2041 tattgatcga tcgttccaac agatccatgc tgtttcacct attatatgcg tatttgaata
     2101 ttttacgaaa aaggctagga aattgtttcc cagccttcaa atccataatt cccatcgtgt
     2161 caacccgtag aaaagatagg agttaatcat tcaactgata tgatataata atataatggc
     2221 tgccctccgg gatgcatttc gatatccaga tcaggatagc tttcctctaa ataggctata
     2281 atctgttcgg cttgttcctg tccaatatct tggccataca ataaggtaac gatctcgctg
     2341 tcatcgtcta tcatcttatc caacaacgag gtcgtaattt tggcaatgtc cttaccggct
     2401 gtaacgatct ttccattggc cagtcctatt atatcgtcct tttcgatggt tatcccatta
     2461 tattccgagt ccctgacggc atatgtcacc tgcccggaat ttacaccttc cattgcctcg
     2521 gtcatattgg tagtgtttgt atctatatcc gaatcgggat tgaaagctaa tatggcagcc
     2581 aaaccctggg gtattgattt gctgggtatc acaacaatat tgctgtcgct tatttcctgg
     2641 gcctgctttg cagataaaat aatattgcta ttattgggca atacgaatac attttttgca
     2701 cttgtccgct ccacagcctt ggatatatct tcaatgctgg ggttcatggt ttgtccccct
     2761 tcgatgacat gatcgacgga gagatccttg aaaatggaac atatcccgtc gcccatagcc
     2821 actgcgacaa taccaaattc cttctgcttt gtctctataa tgacatggcg atgttgttcc
     2881 ttcatgttgt caattttaac gcctgataac tcgccaaatc tcagggctag ctgcaatgct
     2941 ttccccggca tattggtatg cacatgaacc ttgatgagct caggatctcc taccaccaca
     3001 atggagtcac ccagatttgc gagcctctcc tttagtttgt ctacgtcccc ttctgttaca
     3061 tagggaaata aatttttcac aaaaaattcc gtgcaatatg tgaaccggat atctgccccg
     3121 gtcccaatct caggggccat atcatcgaat atctcctctg agacaggcat taattccatg
     3181 tccgagatac cctcaagggc ctgggttgcg cccatcaata tatataaaac acccattccc
     3241 ccggcatcta ctacccccgc ctcttttagg gggggcaaca tatcaggagt cctttcaagg
     3301 gtatctttgg cgacttttat tacctcgtta ctaaaggttt taaaatccgg atgctttttg
     3361 cttaatttta ttgcctcctc acctgttatc ctggcgacgg tcagcattgt cccttcgact
     3421 ggtttcatta cggctttata ggcagtcttc gcaccctctt gcaaggctct tgcgcactca
     3481 cgtgtagaca ttcttgtttc accctgcaat gccctggaaa aacccctaaa tagttgggat
     3541 aaaatgaccc ctgaattccc ccttgcccct tttagggaac ccttggagac agcatctgca
     3601 acttgtgcaa ccgagtttga gtttgtaccc tgcatctctt tgatcgcagc caccatggtc
     3661 agggacatat ttgttcctgt atctccatca ggaacaggaa aaacatttaa ggcgttcagg
     3721 gtctgtttgt ttttttctaa aaactgtgca gccgcccaaa acatttctct tagcttaatt
     3781 ccgtctatat attctatatc caatgcgtta cctcctttgg cgacagaaac ttataccctg
     3841 acaccttcta ccgttacgtt aatttttttc acaatcattc cagttagatt ttcaatgtta
     3901 tacttaactg tttcaataat atttttggct actgcggcaa tggaaatacc atac
//
LOCUS       DASRNX010000154         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_297012,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000154 DASRNX010000000
VERSION     DASRNX010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297012"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..464)
                     /gene="rpmB"
                     /locus_tag="VFD89_06200"
     CDS             complement(273..464)
                     /gene="rpmB"
                     /locus_tag="VFD89_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZJ57818.1"
                     /translation="MARICDICEKGKMSGKNISHSNRKTNRFWQPNIKKIRAMIDGSP
                     KTVKVCTQCIRSGRVERAL"
     gene            1076..>3560
                     /locus_tag="VFD89_06205"
     CDS             1076..>3560
                     /locus_tag="VFD89_06205"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HZJ57819.1"
                     /translation="MIDYGRFKNCPMGYRSAPFWSWNHRLETDEIIRQIRQMNEQGIG
                     GFFMHPRGGLMDEYLGENFMKAIGAAVKEAHDLGMKAWLYDEDRYPSGSAGGMVVENN
                     PEYGAKALEMLEVPYSQIDGMDTNLKVYLPCGDGENASYKDITIISGGDGVEKDALVW
                     VFRIVFMHSQTNCNNKPYTDLCHKDAVAKFIEITHERYSKDLKEYFGNTIPGIFTDEP
                     NFNVITAGKNYLPWTHEFEKSFEDRKGYCIVEHLPELFFMVGDYKKVRFDFWDVMTRL
                     FNESFVQGVYQWCERNNLNLTGHFWEHAFPNPTHGGSVMPHYEFMQYPGIDMLFNTEE
                     EIEQVGNDLIVKEVSSVGNQFGKERILSETFGASGWDIDFAEMKRVADWQFALGINLI
                     CQHLVLYSVEGFRKRDFPSSFLDHQPYWDSYHILGDYLARMSYIMSQGEYMADILVLH
                     PSSSTWSEFDILEDDNPELDGISDSVKELVRTLCQSGYLFDLGDDIIIERHGRIDESG
                     FVIGKRRYSTVILPDMTVMRESNLELLREFAQSGGKIITTGARPYLLDGNHDVGLVNF
                     FNGDDTIKCPIDDLADSLDKLGHGRIIIEGLNGHEVRDIYSHKRVAGNKQIIFLCNYS
                     RNEKHEIRLQLDPAMGKVESWDATGGEKQELFSYTENGKRFIDLLFYPSGSHLLILDG
                     GCPGSTDLTDPSQSAAISKEYKLTHWKVERNDYNALTINRCSVSIDDAQWSEPGNVVA
                     LHDDIRKQLGLDPGNIFAPQPWTYTEKERKRVVPLKAKYTFKLTEHIKGDLRLAAESM
                     DTFIVSVNGRVVENHGTIYKDRAFQVYDIK"
BASE COUNT         1069 a          663 c          832 g          996 t
ORIGIN      
        1 ttttgggata aattaatcgc tattattcta attgtcggtg gggcattgtt aatacttact
       61 tatattccca tatgggcttg ggtagctcta tttgggcttt ttttgatagg tctgggagtc
      121 atgcttctgt tggggcggag gtgatgagcc ttatgagaat ctatgtcgta aaactaccgg
      181 ggtttttagg tagattgata aggaagcttc gtcgcaaaga ctacagatag acaaaaaacg
      241 tgctggtgcg cacgtttttt ccatacccgt aattataggg ctctttctac tctacctgat
      301 cttatgcact gtgtgcatac cttgacagtc ttcgggctac cgtcgatcat tgcccttatc
      361 ttttttatgt taggctgcca aaacctattg gtttttctat tagagtgact gatattcttt
      421 ccggacatct tccctttttc acatatatcg catatccttg ccattttcta cacctccttt
      481 gaaaacaatg gcataaaaga ttagtgccaa tttcaaaagc catatgcttt acgatgtagg
      541 ccccatacat tgccatctat atcgcaaaac attttcattg atccatatgc tgcgaaaagt
      601 acacggtttt aatgaaaatc aaacggtcta actgatttca aatagtgggt atttttcgta
      661 ccatatacaa ataaacatca atattgtacc acttagaagc cgttttgaca agaaaaacag
      721 ataataaaaa atatgaattt aaccggatcc caattgaaaa ctgcaaatag gcttatgctt
      781 taacaagtcg tagaatgtca atcccctggc ggagccacgc tagctttacc gcctgataaa
      841 ccccatagtt tggtgcttta ggatttttat acttgcacca gtgcacagca tcggatttac
      901 agatatttta gtgtcatagc cagtaataat gccggaatta tccagtttca tcttgtcata
      961 ttcggtcaaa tactttaaaa tacacaataa aggggcctca aaatgaaata ttttactggc
     1021 ctcggaaaac atatgggaga gggatacgga ttttaaaact tagggggaaa aatctatgat
     1081 tgattacgga cgctttaaaa actgtcctat gggttatcgt tctgcaccgt tctggtcatg
     1141 gaatcacagg ctggaaacag atgagatcat tcggcaaatc cgccaaatga atgagcaggg
     1201 gataggcgga ttctttatgc atcccagggg cggcctcatg gatgaatatc ttggcgaaaa
     1261 ctttatgaag gctatcggtg ccgccgtaaa ggaggcacat gacctgggga tgaaggcatg
     1321 gctctacgat gaggatagat accccagcgg cagcgccgga ggaatggtgg tagagaataa
     1381 tccggaatat ggtgcaaagg ccttggaaat gcttgaagta ccatatagtc agatagatgg
     1441 gatggacaca aacctaaagg tatatttgcc ttgtggagat ggagaaaacg cttcctataa
     1501 agacataacc atcatttcag gcggtgacgg ggtagagaag gatgctttgg tatgggtatt
     1561 taggattgta tttatgcaca gtcaaactaa ttgcaacaac aaaccatata ccgacctctg
     1621 tcataaggat gcagtagcta aatttattga aatcacccac gaaagataca gcaaagattt
     1681 gaaagagtat tttgggaaca ctatacccgg gatctttacc gacgaaccta actttaatgt
     1741 gattacagcg ggaaagaatt atttgccctg gactcacgaa ttcgaaaaat ccttcgagga
     1801 tcgaaagggt tattgcattg tagaacattt gccggaatta ttttttatgg taggggatta
     1861 caaaaaggtt agatttgatt tttgggatgt catgaccagg ctctttaatg aatcctttgt
     1921 acagggcgta taccaatggt gtgaaagaaa caatctaaat ctcacggggc atttttggga
     1981 acatgcattt ccaaatccta cccatggcgg cagcgtaatg ccacattacg aatttatgca
     2041 atatccggga attgacatgc tctttaacac cgaggaagaa atagagcagg tgggtaacga
     2101 cctaatagtc aaggaagtgt ccagcgtagg aaaccaattt gggaaggaac gaattctatc
     2161 cgagaccttc ggtgcatcgg gttgggatat tgattttgca gagatgaaaa gagtagcgga
     2221 ttggcaattt gcgctgggta tcaatctcat atgtcagcat ctggtccttt attctgtgga
     2281 aggcttcaga aaaagggatt tcccatcttc cttcctggac caccaaccct attgggatag
     2341 ctatcatatc ctaggggatt atttggccag aatgagctat attatgtccc agggggaata
     2401 tatggccgat atcttggtcc tgcatccctc cagttccact tggtcggaat ttgatatatt
     2461 agaggatgac aatccggagt tggatggaat atccgattct gtcaaggaat tggttcgtac
     2521 tttatgtcag tccgggtatc tctttgatct aggtgacgac ataattatcg aaaggcatgg
     2581 gcgaatagac gaaagtggat tcgtaatcgg caaacgcaga tattcaactg tcattttacc
     2641 tgatatgacg gttatgaggg agagtaacct tgaattatta agggagtttg cccaaagcgg
     2701 gggtaaaata ataacgaccg gagccagacc ctatctttta gacggcaatc atgatgtggg
     2761 tttagttaat ttttttaatg gagatgatac aatcaaatgc cccatagatg atctcgcaga
     2821 ttcattagat aaattaggcc atgggcgcat aattatagag ggcttaaacg gacacgaggt
     2881 tcgggatata tactctcaca agagggtagc gggaaataaa caaattattt tcttatgcaa
     2941 ctacagtaga aatgaaaaac atgaaatccg tctccaactg gacccggcca tggggaaagt
     3001 tgaaagttgg gatgccacag ggggagaaaa gcaagaattg ttttcctaca cagagaacgg
     3061 gaaaagattt atagacctgt tgttctaccc atcggggtcc cacctgttga ttttggatgg
     3121 tggttgtccc ggaagcactg atttaacaga tccaagccag agcgcagcga ttagtaagga
     3181 gtataaatta acccattgga aggtagagcg aaatgattat aatgccttaa ccattaatcg
     3241 gtgtagcgtt tccatagacg atgcgcaatg gtcagaacct ggaaatgtgg tagcgctcca
     3301 tgacgacatc aggaagcaat tggggctgga tccgggcaat atctttgccc cacagccttg
     3361 gacctatacc gagaaggaga ggaaaagggt tgttccgtta aaggcaaagt atacctttaa
     3421 actaacggag catataaaag gtgaccttcg cttagctgcc gaatccatgg atacatttat
     3481 cgtgtccgtc aacggaaggg tagtagaaaa tcatggtaca atatataagg atcgagcctt
     3541 tcaggtatat gatataaagg
//
LOCUS       DASRNX010000155         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_189553,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000155 DASRNX010000000
VERSION     DASRNX010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189553"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFD89_06210"
     CDS             <1..209
                     /locus_tag="VFD89_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57820.1"
                     /translation="NVDRYIDDCIEELRRSQLERRRNYLMDQISKMDGMEEHNSIKYK
                     TLVEELSAINRMTKLGPLGKEEVM"
     gene            206..1288
                     /gene="rpoD"
                     /locus_tag="VFD89_06215"
     CDS             206..1288
                     /gene="rpoD"
                     /locus_tag="VFD89_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HZJ57821.1"
                     /translation="MKTGEAGLKRIKELIERGRSKGVLTYKEIMDMLEEIDLEPEQIE
                     KVYDTLETLGIDIVDEESTADDDDEEDEEADQSDDSILEGVNVDDPVRMYLKEIGKVS
                     LLTAEEEVDLAQRMEAGDELAKRQLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGL
                     IKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVSRQL
                     LQEYGREPLPEEIAQEMGITEEKVRDILKIAQEPVSLETPIGEEEDSHLGDFIPDDDA
                     PAPAEAAAFTLLKEQLMEVLDTLTPREEKVLRLRFGLDDGRARTLEEVGKEFNVTRER
                     IRQIEAKALRKLRHPSRSKRLKDFLD"
     gene            1379..2080
                     /locus_tag="VFD89_06220"
     CDS             1379..2080
                     /locus_tag="VFD89_06220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZJ57822.1"
                     /translation="MLSSRLAALAQQIPIGAKIADIGTDHGLMPVYLGKNGVSDRIIA
                     TDISSDSLRKASILIDKENLQDIIETRIGPGLKVIKPGEVDTVVIAGMGGLLISSILD
                     EGAKTLGAVRRLILQPMNNTELVREWLVNNGFAIEDESLVKEGRHIYEIIVSRLGYQG
                     VSDDIYFEVGFKFIENRDPLFQEFLAIKIRKTKAIISKLEKQNTENARNAMVGFSTKL
                     KKYDEVLECFARWEG"
     gene            2056..2889
                     /locus_tag="VFD89_06225"
     CDS             2056..2889
                     /locus_tag="VFD89_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HZJ57823.1"
                     /translation="MFCTVGRIAQMMEEIAPTRWAEEWDNVGLLLGREDSRVEKVMVV
                     LDISPTVVEEAVEKKIDMIIAHHPLIFSPLKRITDGSLEGRLILSLVSHNIAVYCAHT
                     NLDMTAGGVDDTLARLLGLKDINYLDPNTMQTHSKPGFGRWGKLEFPVNLMDFIKSVG
                     KILNTASLDLIKGTGDIKDKLIHIVALCAGSGADYMKYAQNIGADLFLTGEIKYHDAL
                     MAYWLGIDVLAAGHFYTEIPMVKQLINRLQMIMDSLQYKVEIIESKMQRSPYTRIFVD
                     E"
     gene            2949..>3136
                     /locus_tag="VFD89_06230"
     CDS             2949..>3136
                     /locus_tag="VFD89_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57824.1"
                     /translation="MEQLDYLWNYQELDLKIDELDFQKKNSPLRRELYRSIRYLKKQQ
                     QDIEKLTGDVDKKNHIYN"
BASE COUNT         1010 a          517 c          847 g          762 t
ORIGIN      
        1 ataatgtaga tagatatata gatgattgta tcgaagaact ccggcgctca caactggaaa
       61 gaagaaggaa ttatttgatg gatcaaatat caaaaatgga tggaatggaa gaacataatt
      121 ccataaaata taaaacatta gttgaagagt tgagcgccat aaataggatg actaaattgg
      181 gtcctttagg aaaggaggag gttatgtgaa gactggggaa gcaggcttga agcggataaa
      241 ggagttaata gagagaggca ggtccaaagg ggtcctgaca tacaaggaaa tcatggacat
      301 gcttgaagag atagatttag aacccgagca gatagaaaag gtctacgata ctttggagac
      361 ccttggaata gatatagttg atgaagagag taccgccgat gacgatgatg aggaagatga
      421 agaagccgat caatcggacg atagtatctt agagggggtc aatgtagacg atcctgttag
      481 aatgtatctt aaggagattg gaaaggtatc acttttaacg gcggaagaag aggttgactt
      541 ggcccagagg atggaggctg gggatgaact tgcgaaacgc caactggctg aggcaaacct
      601 tcggttggtg gtcagtattg caaaacgcta tgtaggtagg ggaatgctat ttttggattt
      661 aatacaagag ggtaatttag gtctcatcaa agcggttgaa aagtttgatt accggaaggg
      721 atttaagttt agcacctatg ctacctggtg gattagacaa gccattacaa gggctatagc
      781 agatcaagcc aggaccattc gcattcctgt ccatatggtg gagactatta acaaattgat
      841 cagggtgtcg agacaattac ttcaagaata tggcagggag cctctgcctg aagagatagc
      901 tcaagagatg gggattaccg aggagaaggt acgggacata cttaaaatag ctcaagagcc
      961 cgtatcgctg gaaactccca ttggggaaga agaggatagc catcttggag attttatccc
     1021 cgatgatgat gctcctgccc cggctgaggc agcagccttt acgctgttaa aggagcagct
     1081 gatggaggtc ttggatactc ttacccctag ggaagaaaaa gtgttaaggc taaggtttgg
     1141 cctagacgat ggtcgtgcca gaacccttga ggaagtaggc aaagaattca acgtgacgag
     1201 ggagaggata cgacaaatcg aggctaaggc tttaagaaaa ctaaggcatc ctagtaggag
     1261 taagagactg aaggattttc tggattaaga caaaggcacc aattaaaagg ggtgcttttt
     1321 tgtgtagcct gttgtgtggc ctatagcgcc actttaggtc gcatgggggg gaatgatttt
     1381 gttaagctct aggttggcag cgttagccca gcagataccc ataggcgcca aaatcgccga
     1441 tattggaacc gatcacgggc taatgcccgt atacctgggc aagaacgggg tatccgacag
     1501 gattatcgca acggacatca gcagcgattc cctccgcaag gccagtatct taattgacaa
     1561 ggaaaattta caagacataa tagaaacccg tataggaccc ggcttaaagg tcataaagcc
     1621 cggcgaggtt gatactgtcg ttatagcggg gatgggcgga ttgctgataa gttcgatatt
     1681 agatgaggga gctaagacct tgggggccgt tcgcagacta atacttcagc ctatgaacaa
     1741 tacggaatta gtcagagaat ggctggtcaa caacggcttc gctattgagg atgaatcctt
     1801 ggtcaaagag ggaaggcata tatacgagat aatagtatct cggctgggat atcagggagt
     1861 aagcgatgac atctattttg aagttggatt caaattcatt gaaaataggg atcccctgtt
     1921 tcaagagttc ctcgccatca agataagaaa aacgaaggcg ataattagca aactggagaa
     1981 gcagaacacc gaaaatgccc gaaatgccat ggtgggtttc tcaactaagc ttaagaaata
     2041 tgatgaggtg ttagaatgtt ttgcacggtg ggaaggatag cccaaatgat ggaggagatt
     2101 gcacctaccc ggtgggctga agagtgggat aatgtaggcc tgttgctggg ccgcgaggat
     2161 tcaagagtag aaaaggtgat ggtagtcctc gatatatctc caacagtagt agaagaggcc
     2221 gttgaaaaga agatagatat gatcatagcc catcatccat tgatctttag cccgttaaaa
     2281 agaataaccg atggcagcct ggaaggcagg ttgatcttgt ccctagtttc ccataacatt
     2341 gctgtgtatt gtgcccatac caacctagac atgacagccg ggggtgtaga tgataccttg
     2401 gccaggcttc tggggttaaa ggatattaac tatctcgatc ctaatactat gcaaactcat
     2461 tcaaaaccgg ggtttgggag atggggcaaa ttggaattcc cggttaattt gatggatttt
     2521 ataaaatcgg ttggcaagat ccttaatacg gcgagtttgg atttaattaa gggtacgggg
     2581 gatataaaag acaaattaat tcatatagta gctctatgtg cgggctcggg tgcagattat
     2641 atgaaatatg cccaaaatat cggggcagac ctatttttaa ccggggagat aaaataccac
     2701 gatgccctta tggcatattg gctgggtata gacgtactgg cagccggtca cttttatacc
     2761 gagatcccaa tggtaaaaca attaattaac cgtttacaaa tgattatgga tagtttacaa
     2821 tataaggtag agataattga gtcgaaaatg caaaggagtc cctatacacg gatttttgtt
     2881 gatgaataag tgaatatgga tactgacggt ttttggatag aaaagggccg tggaaaggag
     2941 ggaacgatat ggagcaattg gattatttgt ggaattatca ggaactggat ttaaaaatag
     3001 atgaattgga ttttcaaaag aagaattcgc cgctgagaag agagctatac agatcaataa
     3061 gatatttaaa aaaacagcag caggacatag aaaaattaac cggcgatgtg gataagaaga
     3121 atcatatata taatag
//
LOCUS       DASRNX010000156         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_615079,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000156 DASRNX010000000
VERSION     DASRNX010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615079"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VFD89_06235"
     CDS             <1..738
                     /locus_tag="VFD89_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018930575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HZJ57825.1"
                     /translation="ENIINLIRCAWAGSQRYGALLWSGDIHSSFESMRNQLAAGLNVG
                     LAGITWWTTDIGGFHGGNPDDPEFREVFIRWFQYGTFCPVMRLHGDREPHSEPLGTSG
                     GGVCTSGADNEVWSYGKEAYDICKKYMNIREELKPYIKEAMKSAHEKGTPVIRPIFYD
                     FHEDETAWEIEDQYMFGPDLLVAPVMYSKIEKREVYLPKDTQWEDAWTGKVFEGGQTI
                     DVATPIDIIPLFIKKGANLVLKGHRTV"
     gene            complement(1071..2276)
                     /locus_tag="VFD89_06240"
     CDS             complement(1071..2276)
                     /locus_tag="VFD89_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ57826.1"
                     /translation="MKPVKIAMIGVGDISGIYLENIANVFREVELYGVCDLIPERAQK
                     GVSYIKDQQDKGIKINTPKIYADMYEAFEDPEVEVVLNLTRPYEHYEVTKQALLHGKH
                     VYSEKPLGIDVEEGQELVELAQKNGLFMGGAPDTFMGAGIQTARKLVDDGIIGKVIGA
                     NCAMICHGHETWHHDPEFYYKRGGGPMMDMGPYYVTALLQLLGEAEGIMAMTKKSLDK
                     RVITSSPHYGEIIDVDVDTYLTGNILFSNGAIAQIFTTFDVHYSGSAQARFEVYGTDG
                     TLIVPDPNTFGGPVLLLRPEDQKAASGTDPALEAKDVPNFYKGYKEIPLMFDYSKNSR
                     GLGLADMCKAIRTGRGFRADYQQQYHVLEILTSFTKSSEEGKYIPLKTQYNRTAPMAN
                     SLIHGILDD"
BASE COUNT          629 a          504 c          539 g          644 t
ORIGIN      
        1 gaaaatatta ttaacctaat aagatgtgcc tgggcaggga gtcagcgata cggcgcatta
       61 ttatggtccg gtgatatcca ttcctccttt gagagcatgc gaaatcaatt agctgccggt
      121 ttaaatgtag ggcttgcagg tatcacttgg tggacaacgg atatcggggg ctttcatggg
      181 ggaaatccag atgatccgga atttcgtgag gtatttataa ggtggttcca atacggaacc
      241 ttctgccccg ttatgcgtct gcatggggat cgagaacccc attctgaacc gctgggaacc
      301 tccggcgggg gagtatgtac atcgggcgcg gataacgagg tatggagcta tggaaaagaa
      361 gcatacgata tttgtaagaa atacatgaac atcagagagg aactaaagcc gtatataaag
      421 gaagccatga aaagtgccca tgagaagggt acccccgtta taaggcccat cttctatgac
      481 tttcatgagg atgagaccgc gtgggagatt gaggatcaat atatgtttgg accggatctt
      541 ctcgttgccc cagtcatgta ttccaagatt gagaaaagag aagtctacct tccaaaagat
      601 acccagtggg aggatgcatg gacaggtaag gtatttgagg gaggtcagac catagatgtc
      661 gctacaccca ttgacattat tcccctattt attaagaagg gggccaattt ggtgttaaaa
      721 gggcatcgca ctgtataagc attataaaga atgaaagtgg aaccgcggta gatattcagt
      781 atcaacatgt aaattagccg cggttcattc aaatctaatt gtgctcgtta cagccagaga
      841 catattaact caaagaatag acccattaaa tttaaaagga tatgcaaata actggtgtgc
      901 acattccaat gaatcagaat gagtgaacta ttcattcatt aaaactgggg gggtatgtaa
      961 ttatgtttca gatatcgaaa atcattttcc ttgatctata tgttacgaaa actacattgg
     1021 tctattgata ggttttttcg caacatatat aaataacata acgcgctaat ctaatcgtct
     1081 aagataccat gaatcaggct gttggccata ggcgcggttc gattatattg tgtcttgagg
     1141 gggatgtatt tgccctcttc actggatttt gtaaaactgg tgagtatctc cagcacatgg
     1201 tattgctgct gataatcggc acggaatccc cgtccggtgc ggattgcctt gcacatatca
     1261 gcgagtccca atccgcgaga atttttacta taatcaaaca tcaaggggat ctccttgtag
     1321 cccttataaa aattcgggac atccttggct tccagagccg gatcggttcc cgatgctgcc
     1381 ttttgatctt ccggacgaag cagtaagaca ggtccaccga aggtgttggg atcggggact
     1441 ataagggtac cgtccgtacc gtacacttca aaacgggcct gtgcgctccc gctgtagtga
     1501 acatcgaagg tggtaaatat ttgtgctatg gccccgtttg aaaaaagaat attcccggtt
     1561 agatatgtat ccacgtccac atcgatgatc tctccgtaat gggggcttga ggtgattaca
     1621 cgtttatcca gagacttttt cgtcatggcc atgattcctt cggcttcgcc caatagctgg
     1681 agaagagcag taacataata tgggcccata tccatcatcg gaccgccgcc gcgcttatag
     1741 tagaattccg gatcgtgatg ccatgtctca tgaccatgac agatcattgc acaatttgcg
     1801 ccgatgacct tcccgattat accatcatct acaagcttgc gggctgtctg aataccggcg
     1861 cccatgaagg tatcaggtgc gccccccata aagagcccat ttttttgggc aagttccact
     1921 agttcctgtc cttcttccac atcaatcccc aggggttttt cagaatagac atgtttgccg
     1981 tgcaaaaggg cttgctttgt aacctcataa tgttcatagg gacgggttag atttaggaca
     2041 acctccactt cgggatcctc aaaggcttcg tacatatcag cataaatctt tggtgtgttg
     2101 atctttatcc ctttatcctg ctgatccttt atgtaggaaa cccctttttg ggcacgctcc
     2161 gggatcaggt cgcaaacccc atagagctca acttccctaa aaacatttgc gatattttca
     2221 aggtaaatac cgctgatatc gcctacgccg atcatcgcaa ttttaacagg tttcatactc
     2281 tcgttcctcc ttgtacaatt tgttttgtat tagtag
//
LOCUS       DASRNX010000157        13538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_457133,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000157 DASRNX010000000
VERSION     DASRNX010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13538
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457133"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFD89_06245"
     CDS             <1..307
                     /locus_tag="VFD89_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005549527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HZJ57827.1"
                     /translation="LKKGNFMGRDALVEQAEDGPKRRLVGFEMIDRGIPRTGYRVEAD
                     GRDIGFVTSGGMSPTLKKSLGLALIDTDYSGLGNEITVEMRGRAQRAKVVDTPFYVK"
     gene            326..709
                     /gene="gcvH"
                     /locus_tag="VFD89_06250"
     CDS             326..709
                     /gene="gcvH"
                     /locus_tag="VFD89_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZJ57828.1"
                     /translation="MKVLEGLKYAKGHEWVRVEESRAYIGITDFAQDELGDIVFIELP
                     EVGTGVEEGDILGSVESVKAASDIYAPVSGTVIEVNDELEGSPELVNEEPFESWIAVI
                     EMEDTEELNDLMDAEEYQKFCEEEA"
     gene            712..2061
                     /gene="gcvPA"
                     /locus_tag="VFD89_06255"
     CDS             712..2061
                     /gene="gcvPA"
                     /locus_tag="VFD89_06255"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZJ57829.1"
                     /translation="MSRYIPNTKTQQQAMLKEIGYTSIGELFGDIPEGVKLGRELAIH
                     GPMAEMELLRHMQKMASSNKHANGHACFLGAGAYDHYIPAAIDHIISRSEFYTSYTPY
                     QPEISQGTLRAIFEYQTMICELTGMDVANASLYDGATAVAEAVLMACQATRRDKVIVA
                     RSLHPEYRETAATYTKFQDIATIEVGYKDGTVDLKNLEKELNDEIAAVVIQSPNFFGI
                     IEDMDAIAHLAHKNKSLLIACVDPISLGILKSPGEAGADIVVGEGQSLGNPLSFGGPY
                     LGFFATTKRLMRRMPGRVVGETVDRDGNRGFVLTLQTREQHIRREKATSNICTNQALN
                     ALIATIYLSLMGRAGLKEVACQSLQKSHYLYDRLLDTGLFTPIFNGPFFKEFALKSTI
                     PIDKLNAHLLDRGIIGGYSLGRTYPELDGGWLIAVTEKRTRGEMDALVEGVGEIADE"
     gene            2054..3508
                     /gene="gcvPB"
                     /locus_tag="VFD89_06260"
     CDS             2054..3508
                     /gene="gcvPB"
                     /locus_tag="VFD89_06260"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZJ57830.1"
                     /translation="MNRKLIFELSKEGKKGYSLPELDVDGKKIERLIPKELLRHEDAR
                     LPEVSEPEVVRYYTGISRLNHGVDIGFYPLGSCTMKYNPKVNEDISRLEGFSGIHPYQ
                     PQETVQGCLKLIYELNDMLCEITGMAGVTLQPAAGSHGELTGMMIIKAYHVNNGEGER
                     DKILIPDSAHGTNPSSAAMAGFEVVEVPSNDRGSVDVEALRRMADHETAGLMLTNPNT
                     LGLFEDEIEEIAKIVHGVGGLLYFDGANANAIMGITRPGDMGFDVVHLNLHKTFSTPH
                     GGGGPGSGPVGVVKKLIPYLPGPTVQFDGEKYCLRQDSPRSIGRIRSFFGNFGVYLKA
                     YAYILTMGAAGLRHVSETAVLNANYIMEKLKPYFELSYDRICMHEFVLSANRQKEKGA
                     SAMDVSKRLMDFGFHPPTTYFPLIVREALMIEPTETESRETLDEFIDAMIRIAKEIDT
                     DPEKAQTAPHNAPVGRLDETNAARNLRLRWLPDE"
     gene            3597..3854
                     /locus_tag="VFD89_06265"
     CDS             3597..3854
                     /locus_tag="VFD89_06265"
                     /inference="COORDINATES: protein motif:HMM:NF015643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuS family protein"
                     /protein_id="HZJ57831.1"
                     /translation="MKHIAVQEGLGPVINYLKDSGYMVSEFSSRQKSSKDFLDGFDAV
                     VLTGIDDNVMGIQTANNNRPFIEARGMTPEEIKDAIEQRIQ"
     gene            4409..6046
                     /locus_tag="VFD89_06270"
                     /pseudo
     CDS             4409..6046
                     /locus_tag="VFD89_06270"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            6266..6814
                     /locus_tag="VFD89_06275"
     CDS             6266..6814
                     /locus_tag="VFD89_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003836099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZJ57832.1"
                     /translation="MLGYIVAGVVVILVLYGISAYNGFVTFRNRVRNAWAQIDVQLKN
                     RADLIPNLVETVKGYASHEKETFDQIVKARSGVLSANTPEESMEANDMLSRALNRLMV
                     VVEAYPELKANANFLGLQNDLRGVEDKIRYSRQFYNDTVLKFNTRIQTFPNNVVAGLF
                     GFKEEVFFRAEEGDRDVPRVQF"
     gene            complement(6971..7345)
                     /locus_tag="VFD89_06280"
     CDS             complement(6971..7345)
                     /locus_tag="VFD89_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HZJ57833.1"
                     /translation="MDVFGTLIQTGDWKGEKHVPIIHCPENVKAGENFELKVTLGDAI
                     GHPNTLEHYISWFKVYYQADGGKFPIELASFDFGAHGESDVFTDYQGAVTVKLPKSGT
                     VYAISYCNIHGLWGNKVDVAVE"
     gene            7713..8060
                     /locus_tag="VFD89_06285"
     CDS             7713..8060
                     /locus_tag="VFD89_06285"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZJ57834.1"
                     /translation="MFGPSVKKIERWAQKGKIAKLEKIIAGPNVEFREAAFKALGQSG
                     DHEAVTHLTYYVRHPNAEFRRLAAQAMGESGAERTVEFLRKLYRDDEDENVREAANEA
                     ILKINEAQAAETE"
     gene            8114..9481
                     /locus_tag="VFD89_06290"
     CDS             8114..9481
                     /locus_tag="VFD89_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin oxidoreductase/NADH oxidase"
                     /protein_id="HZJ57835.1"
                     /translation="MAVHKKFLYRSLDEIRADIDRLGIEIPILDDPSLLKTPISVNNT
                     TIPNSIGIHPMEGCDGTSHGGPSDLTFRRYERFARGGAGLIWFEATAVTEEGRANPRQ
                     LYICEENKDDLKRLLGETLKASRQEYGQGYKPYTVLQLTHSGRYSRPRKEPAPIIATR
                     NPYLNRNIPDDYPLITDDELKYLEDKFVVAASLAKEIGFDAVDIKSCHGYLISELLSA
                     FTREGEYGGSFENRTRFLLNIVDKINDEIGDELDITLRLNAYDAVPYPYGWGVDKEDF
                     QVPDLKEPVRLVQTLYNRGIGLINISTGNPYYNPHVGRPYDTGYYTPPEHPLTGTARM
                     LDIIRQIQKGVPNMAVMATGFSWLREFAPFVAAGGIQKGWFTIAGFGRQSLAYPSFPR
                     DIITEGSMDRKKCCIACGKCSEIMRDGGRAGCVIKDAKIYAPIYREGRVGKPPVDGSR
                     VAYHV"
     gene            9822..10598
                     /locus_tag="VFD89_06295"
     CDS             9822..10598
                     /locus_tag="VFD89_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HZJ57836.1"
                     /translation="MSGEKVAIITGGSRGIGYGIAERLASEGYNLAIFGTSDEAAVRG
                     ALDRLQAHGGEVLYIQGSLNSHVDRQVLIDRVSSEFGRIDVLVNNAGVAPKVRKDILE
                     TTEESFDFVVDTNLKGTFFLTQGVAKRMVDGAQKGQGYDPIIINIASISSYTSSPSRG
                     EYCLSKAGISMMTKLFAHRLAEYGINVYEIRPGIIHTDMTSAVKDKYDELIDGGLIPI
                     KRWGYPQDVAKAVWALCSGLLPYSTGEVINVDGGFHLRRL"
     gene            10755..12749
                     /locus_tag="VFD89_06300"
     CDS             10755..12749
                     /locus_tag="VFD89_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZJ57837.1"
                     /translation="MLHSIANINDTKINIYSLNTVVVGSGAAGLNAADRLYALGQRDI
                     AIVTEGLNMGTSRNTGSDKQTYYKLTQAGENLDSVYDMARTLFGGGSMHGDIALIEAT
                     LSSRCFYRLVDIGVPFPHNRYGEYIGYKTDHDPRQRATSAGPLTSHYMTQKLQDQVMA
                     KDIKIFDGYQVIGVLTDRGRTRALGLLTLNLNALEDPDNRYVAFNCTNIVYATGGPAG
                     MYGSSVYPESQTGATGIALEAGAVGINLTESQYGIASTRFRWNLSGTYQQVLPRYVST
                     DRDGSDEREFLWDYFKESGKLLDAIFLKGYEWPFDPRKIEDYGSSFIDLLVYHETAIK
                     GRRVFLDYTKNSGGAGNDEILDFSLLGDEAYEYLQRSGALFGTPIERLAQMNRPAIDL
                     YMDNGIDLSKEYLEVAVCAQHNNGGLGGNIWWESNLKHFFPIGEANGSHGVYRPGGSA
                     LNSGQVGGLRAAEFIAARYREGPMDAAEFVDLTRWEIDEKIGLGKKFMANTQDRDDVH
                     SIRKGIGKRMDLWGAHIRSLEGAKRGVGKAREALSTLGEATAISSVLELPEAYKNYDL
                     LTAQYVYLSAISDYIKRGGGSRGSYLICGEEGDREIGGLPQGFEFILDGGRTDGMVQE
                     VTLRDGRPNFKWKKVRPIPKGADWFEDVWKDYRGEVIFDT"
     gene            12829..>13538
                     /locus_tag="VFD89_06305"
     CDS             12829..>13538
                     /locus_tag="VFD89_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HZJ57838.1"
                     /translation="MKQWDIIATATFGLEAVVARELRDLGYKDLKVENSRVIFKGSAF
                     DICRTNLWLRSADRVFIRVGEFSALSFDELFERTKALPWEEWLPEDAEFPVLGKSVNS
                     KLFSVSDCQAIVKKAIVERLKQKYAKSWFEETGPKYTIEVGLLKDIATLTIDTSGVGL
                     HKRGYRELASGAPLKETLASALVGLSYWNTGRQLVDPFCGSGTIPIEAAMMGLNIAPG
                     LNRGFDAERWPQISGKLW"
BASE COUNT         3807 a         2803 c         3679 g         3249 t
ORIGIN      
        1 gcttaaaaag gggaatttta tgggcaggga tgccctggta gagcaggcgg aggatgggcc
       61 caaacgaagg cttgtaggtt ttgagatgat agaccgggga atacccagaa ccgggtatag
      121 ggtggaagcc gacggcaggg atatcggttt tgttaccagc ggcgggatgt cgcctaccct
      181 aaagaaaagt ctgggtcttg ccttaataga taccgactat tccggcttgg gcaacgaaat
      241 cactgttgag atgcggggca gagctcaaag ggcgaaggtt gttgatacgc ctttttatgt
      301 aaaataatta aaggaggaaa ggataatgaa ggtattagag ggattgaagt atgccaaggg
      361 gcatgaatgg gtgagggtgg aagaaagcag ggcctacata ggaattaccg attttgcgca
      421 ggacgaactg ggggatatag tctttattga gctgcccgag gtgggcacag gtgtagaaga
      481 aggagatatc ttagggtccg ttgagtccgt caaggcggct tcggatatct acgctcctgt
      541 ttccggaacg gtgatagagg ttaatgacga actggaaggc agccccgaac ttgtaaatga
      601 agagcctttt gagtcctgga tagcggttat tgagatggag gatacggaag aattaaatga
      661 ccttatggat gccgaagaat atcagaaatt ctgcgaagag gaggcctgat gatgtccagg
      721 tacatcccca acactaaaac acaacaacag gcgatgctga aagagatagg ctatacttca
      781 attggggaat tatttgggga tattcctgag ggagtcaagc tgggcaggga actggccatc
      841 catgggccca tggccgagat ggagcttttg cggcatatgc aaaagatggc ttcttccaac
      901 aagcatgcca acgggcatgc ttgcttttta ggggccggcg cctacgatca ctacataccc
      961 gcagccatag atcatattat ctcaaggtcg gaattttaca cgtcctacac tccctatcaa
     1021 cccgaaataa gccagggtac cttgagggca atatttgaat accagaccat gatttgcgag
     1081 ctgacgggta tggatgtagc caatgcctcg ctgtatgacg gtgccaccgc cgtagcggag
     1141 gctgtcctaa tggcgtgcca ggctaccaga agggataagg ttattgtcgc taggagcctg
     1201 caccctgagt acagggagac tgcggcaaca tataccaaat tccaggatat agcgacgatt
     1261 gaggtgggat acaaggatgg gacggttgat ctcaagaatc tggaaaagga actcaacgat
     1321 gaaatcgcgg ccgtggttat ccaatccccg aacttctttg ggataataga ggatatggac
     1381 gctatagccc atctagccca taaaaataag tcgctgctaa tcgcatgtgt tgaccccatc
     1441 tcccttggaa tcttaaaatc cccgggagag gccggggcgg atatcgtggt gggggaaggc
     1501 caatctctgg gaaatccctt aagctttgga ggcccgtacc taggtttttt tgctactacc
     1561 aagcggctta tgaggaggat gcccggaagg gtggtaggcg aaaccgtgga cagggacggt
     1621 aaccgtggat ttgtcctgac cttgcagacc agggagcagc atattagaag ggaaaaggcg
     1681 acctctaata tctgcaccaa tcaggcatta aatgccctca tagccaccat atacctttcc
     1741 cttatgggaa gggcgggtct aaaagaagta gcctgccaat ccttgcaaaa atcccactat
     1801 ctctatgaca ggctcttaga taccggcctg tttaccccga tattcaatgg accttttttt
     1861 aaggaatttg ccctaaagag cacaattccc atcgacaaat tgaatgccca cctattggat
     1921 aggggaatta taggaggata ttccttgggg aggacctatc cggagctgga tgggggatgg
     1981 ctcatagccg tgactgagaa gcgcactcgc ggggagatgg acgctttagt tgaaggagta
     2041 ggtgagatag cggatgaata ggaaactaat atttgaactg agtaaggaag gtaaaaaagg
     2101 atattcccta ccggagctag atgtggacgg gaagaaaatt gaaaggctta ttccaaaaga
     2161 gctattgcgg catgaggatg cgcgacttcc ggaggttagc gaacccgaag ttgtcagata
     2221 ctataccggc atatcaagat taaaccacgg ggttgatata ggcttctacc cgctgggctc
     2281 atgtaccatg aaatataatc caaaggtcaa cgaggatatt tccaggctgg agggcttttc
     2341 ggggatccac ccctaccaac cccaagagac cgtccagggt tgtctaaagt tgatatacga
     2401 attaaatgac atgctatgcg agattacggg catggcaggg gtcaccctgc agcctgccgc
     2461 gggttctcat ggggaactta cagggatgat gattatcaag gcctatcatg ttaataacgg
     2521 agaaggggaa agggacaaga tactcatccc cgacagcgcc catggcacga atccctcatc
     2581 agcggctatg gcggggtttg aggtggtgga ggtaccctcc aatgaccggg ggagtgttga
     2641 tgtagaagcc ctaaggcgga tggccgatca tgagaccgcc gggcttatgc tgaccaatcc
     2701 caacacgctg ggcctatttg aggatgaaat agaggagatt gccaagatcg ttcacggcgt
     2761 gggaggcctg ttatactttg acggagccaa cgcaaacgcc atcatgggca taactcgccc
     2821 gggggatatg ggctttgatg tggttcattt aaaccttcat aaaaccttta gcactcccca
     2881 cggcggaggc ggcccgggct cgggtcccgt aggtgttgtt aaaaaactaa taccctattt
     2941 gccgggtccc acggtgcaat ttgacggcga aaagtattgt ctccgtcagg acagtcccag
     3001 atctatcggt aggataagat ccttctttgg gaattttgga gtatacctaa aggcctatgc
     3061 ctatatcttg accatggggg cggcgggact gcgccatgtc tcggagacgg ctgtattaaa
     3121 cgccaactat ataatggaaa agctaaaacc ctactttgag cttagttatg accgcatttg
     3181 tatgcacgag ttcgtattgt ctgccaatag acaaaaggaa aagggcgcat ctgccatgga
     3241 tgtatccaaa cggcttatgg actttggatt tcatcccccc acaacctact ttccccttat
     3301 agtgagggag gcgttgatga ttgagcccac cgaaacggag agcagggaga ccctagatga
     3361 attcatagat gctatgatca gaatagccaa agagatagat acggatccgg aaaaggccca
     3421 aactgcgccc cataacgctc ctgtgggaag gttggatgag accaatgccg cccggaatct
     3481 aagacttcgc tggctgccgg atgaataggg agcaaattat tccgacccac agtataatcc
     3541 gaaatggata attaggtttc tatggtgaaa caatagttaa agaggaggtt ttgcctatga
     3601 aacatatagc tgtacaggaa gggttggggc ccgtcatcaa ttatctcaag gattcgggat
     3661 acatggtcag cgaatttagc tccaggcaaa agtccagtaa ggattttttg gacggctttg
     3721 acgctgtcgt actcaccgga atcgatgata acgttatggg aatacaaaca gcaaacaaca
     3781 acagaccctt tattgaggcc aggggcatga cccctgaaga gatcaaggat gctatagagc
     3841 agaggatcca ataaaatacc aatccggtgt gattcacccc gacagacaaa aggccctttt
     3901 ttacgggtct tttgttctta tagacgcccc atggcgaagg ccggcggcag agcccatata
     3961 tctaatggca ccatgccaag tcccatttta gggtgcaatt atcgggctgt ggtcctgttt
     4021 ttttatccat attgggctat agcattttgc tattacatat tataacgcgg ggggtgggta
     4081 taaatctccc gcttctataa aaaattatca gtttcagctg ccgtctttgt ggtaaaataa
     4141 gtatatagaa atccccttgg ggactttata gcatctatcg cccgacagct atcatcaatg
     4201 tatactttac ctacaagacg caatgattta ggctgtagcg ctgtgtatcg tatatttggt
     4261 ggctccgggt gggtcggttg attatttaat gaaaaagggg atttttagga tggattacgg
     4321 atcatagaat agggtcaaat atatctataa gggggtcagg gctatatata aaagaagaat
     4381 catactactt attctattgt gcgggcttat ggccttatct attgtgctgc cgtctgcagc
     4441 gtcaaaggcg gacgagagga gctataatat aagtcactac gacatggtca tagatattaa
     4501 gcctaacggt gatgcccata tcaaagaggg gattacctat gacttctacg gggactataa
     4561 cgggatattc agagacattg acctagatgc cacgggcggt atcaaggagc tagccgtata
     4621 cgtgaaggag ggggacggat ccaggcggtt tcagcaatcc cagggtgagg ggcaggatgt
     4681 atacgagctt acaaatgaag ataaccttac caagcttaag gtctatgaaa aatccagcta
     4741 tgaggaaaag accttcgtat tcgaatatgt gctcttaaac gtagctgaaa ggtataatga
     4801 tattggtgta tttaaccgca aggtcatcga tagacattgg gatgtttact taaacgacgt
     4861 gaatatcacc atcaccatac ccgaaggtgc caagaaggaa gagcttaggg tattcgccca
     4921 cggcccttta acaggggtat cggagattac cgatgagcgc accttttcct ttgctatcac
     4981 cgagataatg ccggaaacct ttatagagac tctggccgta ttcccaccgg ggcttatacc
     5041 tgattccgcc cgggtctacg atgaagtcag attgccccat atcctagaca acgagcagag
     5101 gctggcggac gaagctaatg ccgaaaggga gcgggccagg gaagagctgg agagactgga
     5161 agaggctgaa aggcaaagag cagccaggga agcaagactt aagaggatca gggataggtt
     5221 tctgcccgtc ttcgccttag tcctagttgg gggcatatat gggctgatag tctttatctt
     5281 tagatactcc agggatctta aaccggaatt tcagggcgac tactataggg agcttccggg
     5341 agactatacc cccgccataa tgacctatct tctaaccaag gggaatgtgg attctaagga
     5401 tataatggct actattatgg acctggtgag gaataaaaag atcaatataa ggaagatcga
     5461 aaccgagaag ggactaatat ttaagcgcat tcaggatcaa tatatgattt ccataataga
     5521 cggggtcgac ttggagggtc tataccccca tgaggatttt ttaatatcct ggtttctcta
     5581 taagctgggt acggggggaa gcctaatctt ggacgattta aaggacatat taaagagaaa
     5641 agccagggca ttggaatttc aaagggacta tgaaaccttc aagagtatgg ttaaaagcca
     5701 gggtgagagg caaggattct ttatgaaaaa cgatctaaag ggcagcggga tatacgccct
     5761 aatgggttta atccttctga tctccggcgt tgtctcggca atatatctca aggccttgat
     5821 gggaattatc atggccggaa tagggattgt taccttggtc tctatgttgg tagtcgcagg
     5881 catcagaaag tggaccaggt atggagcgga gcaggcggcc atgtggaagg cctttaggag
     5941 attcttactc catttttcca gcttggatag ggcagagatt ccgtccatcg tcatctggga
     6001 acattatctt gtctacgcca tttcccttgg ggtagcaaaa gaggttatag atcaattgcc
     6061 aagggtattt actgaagccg agctaatgga tccttctcta acctatatgg ggggctatag
     6121 gtccctagga aaccttatga ttatcaacag catgttaaac agcactacag catccataaa
     6181 caacgccata agtacggcgg cggtggcggc gccttctgat ggatatcaaa ttggatatca
     6241 aattattgaa gaaaaggggg attttatgtt aggttatatt gttgcaggtg tagtagtcat
     6301 cttggtattg tacggtatat ccgcgtataa cgggttcgtt accttcagaa acagggtaag
     6361 aaatgcatgg gcccaaatcg acgtgcagct aaagaacagg gcggatctta ttcccaacct
     6421 ggtggagacc gtaaagggct atgccagcca tgaaaaggaa acctttgatc agatcgttaa
     6481 ggctaggagc ggggttttaa gcgctaatac cccggaggag agcatggaag caaatgatat
     6541 gctatcacgg gctcttaaca ggctgatggt ggtggtagaa gcctatccgg aacttaaggc
     6601 caacgccaat tttctgggtc tccaaaacga tcttagaggc gttgaagata agataaggta
     6661 ttcgcggcaa ttctataacg ataccgttct caaatttaac accagaatac agacgtttcc
     6721 caacaatgta gttgcggggt tgtttggatt taaggaagag gtatttttta gagccgagga
     6781 aggggacaga gacgttccca gggttcagtt ctagaagggg gcaaccggac aaattcatag
     6841 cttattcacg ggtgaagccc atgactcccc ggccgaacaa cgggacagaa aaaggataga
     6901 ttgcataggt ttatatgtaa tctatccttt ttttaatgtc agtccattac gttcctagtt
     6961 aattgggttt ttattctacc gctacatcga ccttgttacc ccaaagtccg tggatattgc
     7021 aatagctgat ggcgtaaaca gtaccgcttt tgggtagctt gaccgtaacg gcaccctgat
     7081 aatcggtgaa tacgtcgctc tctccgtggg caccgaaatc aaacgaggcc aattcaatgg
     7141 ggaacttccc cccatcggct tgatagtata ccttaaacca tgagatatag tgctccaagg
     7201 tgttaggatg accgatggca tcgcctaggg tgactttcag ttcaaaattt tcccccgctt
     7261 ttacgttttc gggacaatgg ataattggca catgtttttc acctttccaa tctccggttt
     7321 gaataagggt tccaaataca tccataaaca attctccttc ctatttatct gttttcatat
     7381 acttacccat tcatggagat tccaaatcat gaagatgact taaagccttt ttttcccagg
     7441 gaccggtaca ggtgcttaat ataacacaac gtcctaatta aatacatatc ggtaagaatt
     7501 atttggcgtt taccaagatg ctgctttaaa gaagactcgg gcttacccca ttcgaaaggc
     7561 attttccttg actctaataa gcataccaga tatgattgat atgcggatat tatacaaaat
     7621 aaagagagca acagccgccc cctacaattc acctgtaagg actactgaag gctatcatag
     7681 ctggattaat aaaattttgg gagtggatgc aaatgtttgg accttctgtt aagaagatcg
     7741 aaagatgggc acaaaaaggt aagattgcca aactggaaaa gataatagcc gggcccaacg
     7801 tggaattccg ggaagccgct tttaaggccc ttgggcagag cggggatcat gaagcagtga
     7861 cccaccttac ctattatgtt cgccatccga atgccgagtt ccgtaggctg gcagcccagg
     7921 caatgggcga gagcggagcg gaaaggaccg tagaattttt aagaaaactc tatagggatg
     7981 atgaagacga aaacgtaagg gaagccgcaa acgaagcaat tttaaagatc aacgaagcac
     8041 aggcggcaga gacggagtaa ggggcttaca acctgaaggc cagggggcgg ccaatatatt
     8101 atgggggaat aaaatggcgg ttcataaaaa gtttttgtac cgttctttag acgagataag
     8161 ggcagatatt gacagactgg ggatcgagat ccctatactg gatgatccga gtctgctgaa
     8221 aaccccaatc tccgtgaaca atacaaccat accgaattct ataggtatac atcccatgga
     8281 aggctgtgat gggacatccc atggcggccc cagtgaccta acctttaggc gatacgaaag
     8341 gtttgccagg ggcggggcgg gattaatatg gtttgaggct actgcagtga cagaagaggg
     8401 cagggccaat ccccgacagc tttatatctg tgaagaaaat aaggacgatc taaaacggct
     8461 cttgggcgaa accctaaaag catcacgaca agaatacgga cagggctaca agccatatac
     8521 cgtgctgcag cttactcatt cgggacgcta tagcaggcca agaaaggagc ccgctccgat
     8581 aattgccacc cgaaatccat atctaaatcg caatattcct gacgattatc cgctaataac
     8641 cgatgatgaa ctcaaatact tagaggataa atttgtagtc gctgcatctc tggctaagga
     8701 gataggcttt gatgcagtgg acataaagag ctgccacggt tatttaatct ccgaactcct
     8761 gtccgcattt actcgagaag gcgaatacgg gggaagcttt gaaaatcgta ctcggttttt
     8821 attgaatatc gtggataaga tcaatgatga aattggggat gagctggata taaccctgag
     8881 gctaaacgcc tatgatgctg ttccctatcc ctatggctgg ggtgtagata aggaagactt
     8941 tcaagttccg gatctgaaag aacccgttag gttggttcaa accctgtata atagggggat
     9001 tgggctcatc aatatcagca ccggcaatcc ctattataat ccccatgtag gcaggcccta
     9061 tgatacgggt tactatacgc ctccggagca ccccttaacg gggacggcca ggatgctgga
     9121 tataatcagg cagatccaaa aaggtgtccc aaatatggcg gtaatggcca ccgggtttag
     9181 ttggctcagg gaatttgctc cctttgtggc agctggagga atacaaaagg gttggtttac
     9241 catagccggg tttggacggc aatccctagc atatccaagt ttccctaggg atattataac
     9301 cgagggcagt atggacagaa agaaatgctg tatagcttgc gggaagtgtt cggagataat
     9361 gagggatggg ggcagggccg gttgtgtaat caaagatgcg aaaatatacg cccctatata
     9421 tagggagggg cgggtaggta aaccgccagt tgacggaagc cgggtggcgt atcatgtgta
     9481 gcatgacgct atttataagt ctacatacag ggtttaccaa aagaaaattc accagctagc
     9541 gctatgggca gagccggaac cggagcaccg gaaccggttc tttttaatct atgattaaaa
     9601 catcggaagc aattgcattg gcaattgcaa cacacaggtt tcaacggggc ggaagactta
     9661 tacccactgc ccctgttcta ctaatgggaa ctcgccgctt atagaagcgg gggcatcttt
     9721 ccgccccgtt atatttgtat agtggcggtt acgatatttg tggcattata aaagatcaat
     9781 atatctggtg tagcaaaaaa catggaggga gaccttgaag gatgtctggg gaaaaagtag
     9841 cgattattac ggggggcagc cggggaatag gctacggaat agccgagagg ctggccagtg
     9901 aaggctataa cctggctata ttcggtacgt cggatgaggc agcggtcaga ggggctttgg
     9961 accggctgca ggcccatggg ggagaggttt tatatatcca aggaagcctt aatagccatg
    10021 tggataggca ggtgctaata gatcgtgtat catcggagtt tggaaggatc gatgtcttgg
    10081 taaataacgc gggagtagca cctaaggtaa ggaaggatat attggaaact acggaggaga
    10141 gcttcgactt tgtagtggat acgaatctga agggtacctt ctttttgacc caaggagtag
    10201 caaaaagaat ggtagatggg gcgcaaaaag gtcagggtta tgaccccatc attataaata
    10261 tcgcatccat atcctcctat acctcctcgc ccagtcgggg cgaatattgt ttatccaagg
    10321 cgggcatcag catgatgacg aagctctttg cccacagact ggccgaatac ggcatcaacg
    10381 tttatgaaat aagaccgggt ataatccaca ctgatatgac cagtgctgtg aaggataaat
    10441 atgacgaact gattgacgga ggcctaatcc ctataaaacg ctggggttat ccccaggatg
    10501 tagccaaggc ggtatgggcc ttatgctcag gcttgctccc ctattccaca ggggaggtca
    10561 taaatgtgga cgggggattt catctgagaa gattataacg agtcggcaag atgtctcccg
    10621 cttctataag cggcgagtcc caattagtaa ggtataacag acggtgggta taaatccccc
    10681 gcctcgttga aacctgtgtg ttgcaattgc caatgcaatt gcttccgatg ttttgaacaa
    10741 ggagggatgc gggattgttg catagcatag ccaatatcaa tgatacaaag ataaatatct
    10801 attccctaaa tacggtggtg gtgggctcgg gagcggcggg actcaatgca gcagacaggc
    10861 tatacgcctt gggtcagagg gatatcgcca tcgtcacgga agggctcaat atggggacgt
    10921 ctaggaacac cggatccgac aaacagacct attataagct gacccaggct ggggaaaatc
    10981 tcgattcagt gtacgatatg gcaaggaccc tcttcggcgg gggcagtatg cacggggata
    11041 tcgcgctgat agaggctacc ctatccagcc gatgctttta tcgtttggtg gatataggtg
    11101 tcccatttcc ccacaatcgc tacggggagt atataggcta caaaaccgat catgatccca
    11161 ggcagcgggc tacctcggcg ggccccctga catcccatta catgacccaa aagcttcagg
    11221 atcaggtgat ggcaaaggat ataaagattt ttgacggata tcaggttatt ggtgttctga
    11281 cggatagggg taggacccgg gcccttggac tccttacctt aaacttaaat gccctagaag
    11341 acccagacaa caggtatgta gcctttaatt gtacaaatat agtatatgcg acagggggcc
    11401 ctgcggggat gtatgggtca tcagtttatc ccgaaagcca gactggagct acgggaatcg
    11461 cactggaggc aggtgctgtt ggaataaatc tgacggaatc ccagtacgga atagcgtcta
    11521 caaggtttag atggaactta tcggggacat accaacaggt gcttcccagg tatgtgtcta
    11581 ccgataggga tggtagcgat gagcgggaat ttctgtggga ttattttaaa gaatcgggaa
    11641 agcttttaga tgctatattt ttaaagggtt atgagtggcc ctttgacccc agaaaaatag
    11701 aagactacgg ttcatccttt atagatctgt tggtctatca cgagacagcc ataaagggga
    11761 gaagggtatt tttagactat accaaaaact ctgggggagc cggtaatgac gaaattctgg
    11821 atttctccct tttaggggat gaggcctatg aatacctcca aagatcgggg gccctatttg
    11881 ggacccccat agagaggctt gcccaaatga atcggcccgc aatcgatcta tacatggata
    11941 acggcatcga cctttctaaa gagtacctgg aggtagcggt atgcgcccag cataataacg
    12001 gcggtttggg ggggaatata tggtgggaga gcaaccttaa gcattttttc cccatcggtg
    12061 aggccaacgg atcccatggc gtatatcgcc ccggggggag cgcactgaat tcgggacagg
    12121 tagggggcct gagggctgct gagttcattg cggctaggta tagggaagga cctatggatg
    12181 cagcggaatt tgtggattta acccgttggg aaattgacga aaagatcgga ctcggtaaaa
    12241 agtttatggc caatacccaa gaccgggatg atgtacatag catcaggaag ggaataggca
    12301 aaagaatgga cctttggggt gcccatatac gctctttaga gggtgcaaaa aggggagtag
    12361 ggaaggcccg ggaagcgtta tcaaccctgg gcgaggctac ggccatatct tcggttttgg
    12421 agcttcccga agcatataaa aactatgacc tcttaacagc ccaatacgtt tacctatccg
    12481 ctatctcgga ttatattaaa agaggtggcg gaagccgggg ctcttatcta atctgcgggg
    12541 aagagggaga ccgggagata gggggactac cccagggatt tgagtttatt ttggatgggg
    12601 gcaggacgga tgggatggtc caagaggtaa ccctgcgaga cggaagacct aattttaagt
    12661 ggaaaaaagt ccggcctatt cccaaggggg cggattggtt tgaggatgta tggaaggact
    12721 atcgtgggga agtgatattt gacacatagt ctcacctgtt gcgctagttc attaccaata
    12781 tatgtcaaac tgttatagct ggttagttta taaagaaaga ggatggatat gaagcaatgg
    12841 gatataatcg ctactgccac cttcggttta gaggcggtag tagctaggga actgagggat
    12901 ttaggatata aggatttaaa ggtggaaaat tctagggtga tattcaaggg ttcggccttt
    12961 gatatatgcc gcaccaacct atggctcaga agcgcggaca gggttttcat aagagtaggt
    13021 gagttcagcg cccttagctt tgatgaattg tttgaaagga caaaggccct accttgggaa
    13081 gaatggttgc ctgaggacgc agagtttccc gtgctcggca agtccgtcaa ttctaaactg
    13141 ttcagcgtct ccgactgcca ggccatagtg aagaaggcca tcgttgaaag acttaagcaa
    13201 aagtatgcca agagctggtt tgaagagacc ggacctaaat acaccataga ggtcggactg
    13261 ctcaaggata tcgccaccct gaccatagac accagcggtg taggcctcca taaaagggga
    13321 tatagggagt tggccagcgg ggccccccta aaagagacct tggcatctgc gctggtggga
    13381 ctcagctatt ggaatacggg aaggcagctc gtagacccct tttgtggttc tggcaccatc
    13441 ccaatagagg cagccatgat ggggcttaac atagcccccg ggttaaacag gggatttgat
    13501 gccgagaggt ggccccaaat ctccggcaag ctatggga
//
LOCUS       DASRNX010000158        19759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_719099,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000158 DASRNX010000000
VERSION     DASRNX010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 19759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19759
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719099"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..569)
                     /locus_tag="VFD89_06310"
     CDS             complement(87..569)
                     /locus_tag="VFD89_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZJ57839.1"
                     /translation="MKKVISILLMLMLVVSVFAGCGGDKKGDDSKKDDGAAALKDGTY
                     TAEEPDFDEKSGWKDNVTLEVKDGKIASVDWNSTHKDGGKDKKTSSKDGDYGMVAHGD
                     AVAEWHEQAEKVEAFLIEKQDVNAIELKDGGKTDTVAGVTMIVGGFVDMVKDALSKAK
                     "
     gene            1060..2859
                     /locus_tag="VFD89_06315"
     CDS             1060..2859
                     /locus_tag="VFD89_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZJ57840.1"
                     /translation="MSSKHIIILGAGYGGLQAAKTLHKRLRKHDDVRITLIDQNNYHT
                     LLTELHEVAGHRIEPNGVQISIEHVLEYTKVDFVKDQIVGADLKAKKLFSDETQYDFD
                     YLIIGIGSEPAYFNIEGMEDHSFPLWSLDDAKRIQRHITHMFELASHEEDADKKKEML
                     TFVVGGGGFTGVEMMGELIEWVKTLCKKYEIPREDVRLVQVEGLPHLCPILDSKLVTK
                     VERYLQKKGVEVLTDSFITGVTPDSLTIGEAKTIPTRTVIWTGGIKANAFVKDLGISL
                     GNRDRIATNRYLQTKEYPYVYAVGDNMEFFDADDEILPPLVETAVQSADCAAFNIAAD
                     IKGGDKKGLKANLHGVMVSIGSFYSVAQLSGMPLLAGFHATLIKHLVNIHYLFGIGGI
                     ELILEYLSHQFFYKSRRPNRLLETGMGHIRRRNFTLWLVPLRIWLGVMWLVSGIQKVQ
                     NDWLGSWPMIGLTGSADATASASITPLIGPNTPNWYKAIAEKLIMPNALFFQKAIVMV
                     EIGLGIAFILGIFTFIAGIASIGMHINFALGAGLPQANGLPDLWWIMASFAMFAGAGR
                     AFGIDYYLIPFLRNQLRYLQSNKRLNILKGWEL"
     gene            complement(3071..3490)
                     /locus_tag="VFD89_06320"
     CDS             complement(3071..3490)
                     /locus_tag="VFD89_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HZJ57841.1"
                     /translation="MNRKLYRSRKQRMLAGVCGGIAEYFGIDVTIVRLIWAVLTLPTF
                     GSGLLIYLIGAIVIPEKPFSEQDYDPVASEEVFPPVDRTKIMSILGLVLVVVGIMALI
                     SGMFPVLWRLVKNGLWPAIIIIIGILLIHSSWKNSGK"
     gene            3797..4084
                     /locus_tag="VFD89_06325"
     CDS             3797..4084
                     /locus_tag="VFD89_06325"
                     /inference="COORDINATES: protein motif:HMM:NF018633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HZJ57842.1"
                     /translation="MEEERDILVFVDEDGEEIEMEVLDYFYHEDQEYALLTTYDEDCQ
                     CEDCDCEEKELYIMKVVLDGDIEEFEPVEEERMESLIQAVEALYYEDDEEL"
     gene            4226..5302
                     /locus_tag="VFD89_06330"
     CDS             4226..5302
                     /locus_tag="VFD89_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ57843.1"
                     /translation="MQRTDGMNYAPKGKPKPVCEKGDFVFSAIALDHGHIYGMCNGLT
                     EAGGLLKSVYDPDPQKVEAFCEAFPGVKVASSEEEILGDKEVMMVAGAAVPSERAPLG
                     MRVMDSGKDYFTDKTPFTTLEQLDLARAKAVKTNKKYMVYYSERLHTESGVFAGELIK
                     DGAIGKVIQVLGLGPHRLNALDRPGWFFEKEKYGGILCDIGSHQAEQYLYYAGEKDAT
                     VVKSQVANYAHPQYPELEDFGEAMILGENGTSNYFRVDWFTPDGLRSWGDGRTTILGT
                     KGYIELRKYIDIARHNTPDHLFLVDQEGEHYFDCSGKVGYPFFGELILDCLNRTENAM
                     TQEHAFKAAELCLKAQADAVRISG"
     gene            complement(5404..6120)
                     /locus_tag="VFD89_06335"
     CDS             complement(5404..6120)
                     /locus_tag="VFD89_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZJ57844.1"
                     /translation="MSKKALIVQGGWDGHEPVQVSDIFKGLLEESGFQVELSDTLDSF
                     LDGEKLKGLDLIVPVWTMGQITDDQMWPVVEAVASGVGIAGCHGGMCDAFRNCVQWQF
                     MTGGNWVSHPGSDGVEYMINIKKGSSPIVEGIEDFPVKSEQYYLHVDPSIEVLATTRF
                     PVVDGPHATNGEVDVPQIWTKRWGAGRVFYNALGHHADIFDIKPALEIMRRGFIWAAE
                     GKEIAKKEGAKVYENLRKMF"
     gene            complement(6275..7741)
                     /gene="xylB"
                     /locus_tag="VFD89_06340"
     CDS             complement(6275..7741)
                     /gene="xylB"
                     /locus_tag="VFD89_06340"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HZJ57845.1"
                     /translation="MYYIGIDLGTSAVKLLLMSGDGTILKSVSKEYPISFPRPGWSEQ
                     NPEDWYENTLEGLSELLSGIDKSRVAGIGFGGQMHGLVVLDEEDRVIRPTILWNDGRT
                     AEETDYLNEKIGKERLSKYTANIAFAGFTAPKILWMKKHEPENYKRIHKIMLPKDYIA
                     YRLTGVHCTDPSDASGTLLFDVENRRWSEEMMEICGVTREQLPDIFESYEVVGTLKGE
                     TAEELGLNKDVKVVAGAGDNAAAAVGTGTVGEGKCNVSLGTSGTIFISSERFGVDENN
                     ALHAFAHADGNYHLMGVVLSAASCNKWWMEDILDTEDFHGEQEGIENLGKNSIYFLPY
                     LMGERSPHNDPNAKGAFIGMTMETGRKDMTQAMMEGVAFAIRDSYEIAKALGIRVERT
                     KMCGGGAKSPLWRRILANVLNLKVDILEAEEGPALGGAMLAAVGCGEYGSVEEAAERL
                     VRTVLTVEPDPDLVSLYNERYKVYTDIYPRLKGIFREM"
     gene            complement(7849..9168)
                     /gene="xylA"
                     /locus_tag="VFD89_06345"
     CDS             complement(7849..9168)
                     /gene="xylA"
                     /locus_tag="VFD89_06345"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HZJ57846.1"
                     /translation="MSEIFNEIPKIKYEGPSSKNPLAFKYYDEDKVILGKTMKEHLPF
                     AMAWWHNLSATGADMFGVGTADKSFGAQVDTMEHARAKVDAGFEFMEKLGIEYFCFHD
                     ADLVPEAATIAETNARLDEITDYIKEKMDATGKRLLWGTANMFTNPRFMNGAGSTNSA
                     DVYCFAAAQVKKALDLTVKLGGKGYVFWGGREGYETLLNTDMKFELDNIAKLMKMAVD
                     YGRSIGFEGDFFIEPKPKEPTKHQYDFDAATAIGFLKEYGLDKDFKLNIEANHATLAG
                     HTFQHELRVSAINGMLGSIDANQGDYLLGWDTDQFPFDVYEATLCMYEVLKAGGLENG
                     GFNFDAKNRRPSYTHEDMFHGFILGMDTFALGLIKAAQIIEDGRIDDFVKERYSSFDS
                     GIGAKIKNGETSLVELAEYAEKLGAPELPGSGRQEYLEGVINSILFG"
     gene            complement(9372..12539)
                     /locus_tag="VFD89_06350"
     CDS             complement(9372..12539)
                     /locus_tag="VFD89_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HZJ57847.1"
                     /translation="MLEAFKGFHKKPDYHNQYITQINRAPAHFPWSAYESADQARLGG
                     DSKFTFSLDGEWDFLLVDNPESMPAGFNQPDAELSEWGKIKVPGNWELQGYGKPVYVN
                     TLYPFKDKVGEKYLLDIGKKKDYDIYQRYNPPYVPEENACGIYRRSFTLPESFGDRSV
                     FINFGGVESAYYLWINGHPVGYSQDSKLPSEFEITEYLNSGENIITLAVLRFCDGTWL
                     EGQDYFHLSGIFRSVNLIAKPVLRIQDFKVDARPCGNGGGIINAHCFVNRTEGFADSS
                     VRLSLFDEKGRLLAERTKPVDTTSPIMGMGSGWGFKGMRPISESAFFELEVDDVLPWD
                     FDRPNLYRVVFTLIDGVGDEKDFEALNIGFREVSIENNVIKLNGKRIVFRGTNRHEHF
                     WLTGRTVSREHMIEEIKLMKRLNFNSVRTSHYPDDPIWYDLCDKYGLMVVCETNLETH
                     GLGGNLTNNPEWAEAMLERARRMVLIHKNHPSIVSWSLGNESGYGAGHAAMANWIREY
                     DDTRLVQYENNDPGLIASDIKCTMYPTMELLDYMIADNNDRRPIVLIEYAYQITNSSG
                     HFEQFNRLAEKYEIFQGGFVWDWQDKCLPAVNEDGETFFGFGGDWGEDLTDWVCPYYM
                     CANGIVMADLTPKPSGLELKQAQSPIIIEPDEGVCGGYIARNRTQSMGFDELTLEYEI
                     LVHGERIDGGDIHLPIADGPCGDIAFRVDLSPVEDETDEVYINIRVKTADDYLWAKKG
                     HEIANYQFEIKGPAPMVPKMSADGGDLSIEEADNLLTVKGQDFKVIFDLEENTLHSYM
                     RKGKEYILRSGVENFDRSRTGLHLESKWWGESDDLWPSFMPGKLKRNPLAMDYGISQR
                     EGSARIAFTNRIDGDKGSIYSRVSYTVYRDGDIGVSAAIDIDSNYVHLPRVGLGFIAP
                     EGFNTLHWYGRGPGESYCDRKLAAPVGKYSSSVEDTHFPFVPVSHNGSHADTRWFTLE
                     DEDGSRITVKGSSFSFDVHHNTVEDYWNNYHEHELVRRDEVYINIDGAMAGIGGDMAW
                     STQLDPKHKVLADKHQFEFLMSFSG"
     gene            complement(12620..14077)
                     /locus_tag="VFD89_06355"
     CDS             complement(12620..14077)
                     /locus_tag="VFD89_06355"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HZJ57848.1"
                     /translation="MRKSRNLIIVLVVLGVLAGSYFFLANRPEPEGPEEVEKISVLKL
                     EKDEIQKMEIKNEFGELTFTRGEKEVEVEKDGKKEKEKKSVWEVDYPHEILLKEMRVD
                     DLAYSFANLQAERIIEEETPDDLAPYGLKEPQATGTVTLKDGSKNILYLGNQTPIGNT
                     YYLMLDGDPKVYEVWTNHGDHLRYTLADVRDKSLPQVNPEALSYLLVDKKDDRPIEIR
                     LSEEDEEDAVQVNLGLWQMIRPYRETMNVNGEAVSKYLEAMPMLSIEDFVEDGAEDLS
                     KYGLDEPKAEFIIKDDENSIHLLIGDEYDDGMVYFKLPDSNSVYGMKKANTKFLDVTA
                     FELVDKFIYIVSIDNVDKIEVEGLGQKHTLTLERETKPAEEEDEEDEVVTTYRVDGKE
                     VEDKPFKKVYQSVVGVLADSEVKPGAAGRPEIKATFHLNKGKNRQMLINYVPYDNDFY
                     AVFIDGESEFAVNKPMVHQMLTDIGALYRGEFKVD"
     gene            complement(14074..15516)
                     /locus_tag="VFD89_06360"
     CDS             complement(14074..15516)
                     /locus_tag="VFD89_06360"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HZJ57849.1"
                     /translation="MKKLNRIKILDSFKNKKFKYGGYATLMTAFVLAILVIINLVVGQ
                     IPFRVDLTENRLFSLSDQTDTILKNLDQKIEIIGLYQTGNEDKVFDEILQRYRRGNKN
                     ISISYVDPVKNPTFSSKYTKDGTSLRDGSYIVETEDKYKVIDYYDLFNVKTDQYGNTR
                     AESLAMEERVTSAIQYVTAEELPVVYTLEGHMEEALPYELRKQMELENYEIKTLSLLT
                     EDQVPEDASVLMIIAPQRDMTGEEEEKVREYLENKGRALFLMDIVEDEMPNFDALLKS
                     YGVALNKALTVEGDKNHYFQHPAWIVPNMQSHGIISPMKANKMQVLAMGAQGIIELDM
                     KKRSIEIEPLLITSNDAWGRADMKSTAPEKEEGDLDGPFNIAVAIADKTWDEKVGKYD
                     ETKLVVMGDAEFIDPRFASIGNADFVLNSLNWLQDKQESISIRPKSMLSQYLNINAFQ
                     RLLFAGIAVILIPLLILGAGLVMWLRRRHL"
     gene            complement(15529..16395)
                     /locus_tag="VFD89_06365"
     CDS             complement(15529..16395)
                     /locus_tag="VFD89_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZJ57850.1"
                     /translation="MLAIYKRELRAYFTSSTGFIFMGFFLILSGFFFAMTNLFPASPN
                     YNSVLGSITFIFLIVVPILTMRLMPDDKRQRTDQLLFTSPLSLTGIVLGKYLAAVSVF
                     VLTLLITCVYPIILSFFGNLAIWEIVGGYIGFLLLGSAFIAIGLLVSTFTDNQVIAAV
                     ITFSALLFMWIIDWVVQGLPTDRTSGIIFAGIIALALGAFVYFNTRNIYVGVATALVG
                     AAAIVLTYFLADRYFFDGFIVRVFEWFSLLKRYEEFQMGILSLGPIVYYLTFSAAFVY
                     MTVRVLEKRRWS"
     gene            complement(16397..17347)
                     /locus_tag="VFD89_06370"
     CDS             complement(16397..17347)
                     /locus_tag="VFD89_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ57851.1"
                     /translation="MIEIQNVTKRFGQHLAVDNLDFTVNEGEILGFLGPNGAGKSTTM
                     NIITGYISATEGTVTVDGYDILDNPEEVKKRIGYMPEFPPLYVDMTVREYLDFVFEIK
                     KVDDKDKKQDMDRILELVRITDVRDRLIKNLSKGYKQRVGLAQALVGNPQVLVLDEPT
                     VGLDPKQIIEIRNLIKQLGKKHTIILSSHILPEVSAVCQRLVIINKGRIVASDTPDNL
                     SKRLGDSSKMSLRIAASERQAVKVLKDVAGVKYVEAQGIMEPDTIDVWVEAEKDIDVR
                     RPLFNALSRAGYPILQMKSMDLTLEDIFLQVTTQEKVEVS"
     gene            17670..18503
                     /locus_tag="VFD89_06375"
     CDS             17670..18503
                     /locus_tag="VFD89_06375"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HZJ57852.1"
                     /translation="MLKIGSHLSVAKGFEAMGLDAIKIGANTFQFFTRNPRGSKAKDI
                     DPEDAQKLKDIMRENDFAKILAHAPYTLNPASNKENVREFARLIMEDDLRRMEYFPNN
                     LYNFHPGSHVGQGVPKGIEYITKLLNEILKPEHTTTVLLETMAGKGTEIGRNFEELNA
                     IIDGVKLSEKVGVCLDTCHVYDGGYDIVNDLDGVLEEFDRVIGLNRLHAIHINDSKNP
                     FKSHKDRHEKLGQGTLGLETIVRIINHPMLRHLPFYLETPNELEGYAEEIRVLRRSYS
                     P"
     gene            complement(18680..>19759)
                     /locus_tag="VFD89_06380"
     CDS             complement(18680..>19759)
                     /locus_tag="VFD89_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57853.1"
                     /translation="LYTDEDGNYAWDVPEGLWQVQYELDGYETIYSNWLPVPPPQTDV
                     NIDMISITAPKLESLNVFADRIDIVFDKYIDPATVNGILIKDKTGGKIGYKIQYSEEE
                     TSENGTVYTETFVLKFDSKQSINDIMSIDIPNTVQSYSGVPIEAYSKNFLSDDPDGDQ
                     GWKKNSNGEWYYLSEDGKMVTGWMLDADKWYFMDEKTGIMQTGWLKSGGKWYYFNKSG
                     VMQSGWVPVGGKWYYMDSSGAMTTGWQKVGGKWYYMNASGAMTTGWQKVGGKWYYISA
                     SGAMTTGWQKVGGKWYYMGASGAMTTGWQKVDGKWYYMDKSGIMQIGWLLSGGKWYYL
                     YPDGSMAVNTTIGKYRIDNKGVWTA"
BASE COUNT         4895 a         5084 c         4209 g         5571 t
ORIGIN      
        1 gtcctatttt tttcgctagt attttcatat aaaaggccca cccaagcggg caggcccttt
       61 actgcaattt aatttttata gagcgattat ttggcttttg agagagcatc ctttaccata
      121 tccacaaatc cgccaacaat catagttacc ccggcgacgg tgtcggtttt cccgccgtct
      181 tttaattcaa tagcgttgac atcctgcttt tctatcaaga acgcctccac cttttcggcc
      241 tgttcgtgcc attcggctac ggcatctcca tgtgccacca tgccgtaatc cccatccttg
      301 gaagaagttt tcttgtcctt acctccgtct ttatgggtgc tgttccagtc aacgctggcg
      361 atctttccgt ctttaacctc aagggttaca ttgtctttcc aaccactctt ctcgtcaaag
      421 tctggctctt ccgccgtgta ggtgccatcc tttaaagctg cagcaccatc gtcctttttg
      481 ctatcgtcgc cttttttatc cccaccgcaa ccggcgaaga cagagacaac gagcattaac
      541 ataagtaaaa tactaatgac ttttttcata cctgagacct cctattattt aattaatcag
      601 tccctataat cgtctccatt gtttcgccaa tgttaggggc atgattttta taattatgca
      661 aattgtatca caatgtcctt ggcttttcat agtccgaata tatccccatg atccttgttt
      721 tgtagcattt atccgtatat atccaaacta catccgcaat acgtaatgta ccattgaata
      781 tattcgtata tctgcccttt ttgtttttag atttatcaga tatccatcta taatatcttt
      841 tttttgttat aaaataaact tatagcccaa taataggatt taagctgatt tatgtccttt
      901 ttaacgggta tcatttatgt atgttatgaa taaacaactg cagctataaa atttatagcc
      961 acgaagttcc atagaaccgc tgtaactgtc gtgccgataa atttagtttc cgtaatgttt
     1021 atctatatag attcgcatat ttaggaggat ggattcttta tgtcaagcaa acacattatt
     1081 atactggggg cgggatacgg cgggctccaa gctgccaaaa ccctccacaa aagacttaga
     1141 aaacatgatg atgtcaggat aaccttgata gatcaaaaca attatcatac attgctcacg
     1201 gagcttcacg aggtggcagg tcatcgcatc gagcccaatg gagtccagat atccattgag
     1261 catgttctgg aatatactaa agtggatttt gtcaaagacc aaatagtagg ggccgaccta
     1321 aaagccaaaa agctcttctc cgatgaaacc caatacgatt ttgattatct aataatcggg
     1381 atcggcagtg aaccggcgta tttcaacata gagggtatgg aggatcattc tttcccgcta
     1441 tggtccttgg atgatgcgaa gaggattcag cggcatatca cccatatgtt tgaactcgcc
     1501 tcccatgaag aggatgccga caagaagaag gagatgttga ccttcgtggt cggcggcggc
     1561 ggatttaccg gcgtcgagat gatgggcgaa ctcatagagt gggtaaaaac cctgtgtaag
     1621 aaatacgaga tcccaaggga ggatgtgcgg cttgttcagg tggaggggct cccgcaccta
     1681 tgcccgattc tggattctaa gttggtgacc aaggtagaaa gatatttaca gaagaagggt
     1741 gtagaagtcc tcacagattc ctttattacg ggggttacgc ccgatagcct gaccatagga
     1801 gaggcgaaga ccatccccac aaggaccgtc atatggaccg gcggcatcaa ggccaacgcc
     1861 tttgtaaagg atcttggtat ctccctcggc aatcgggata ggatcgccac caacagatac
     1921 ctgcagacca aggagtatcc atatgtctat gccgtcgggg ataatatgga gttttttgac
     1981 gcagatgacg agatccttcc gcccttggta gagacagccg tccaatctgc ggattgtgca
     2041 gcttttaaca tagctgccga tataaaaggc ggcgataaaa aaggactaaa ggccaacctc
     2101 cacggggtta tggtatctat aggttccttt tattccgtag cccagctgtc gggtatgccc
     2161 cttttagcgg gttttcatgc aacccttata aagcaccttg taaatattca ttatctcttt
     2221 ggcatagggg gcatagagct tatcttagag tatctcagcc atcagttttt ctacaaatcc
     2281 agaaggccca acaggcttct ggagacggga atgggccata tacgccgaag gaattttacc
     2341 ctctggctgg tgccccttcg catttggctg ggcgttatgt ggctggtctc cggcattcaa
     2401 aaagtgcaaa atgattggct gggatcttgg cccatgatag ggcttacggg atccgcagat
     2461 gctacggctt ctgcttcaat caccccccta ataggaccta ataccccaaa ctggtataag
     2521 gccattgcag agaagctgat tatgcccaac gccctattct tccaaaaggc catagtcatg
     2581 gtagaaatcg ggcttggcat cgcctttata ttggggattt tcacctttat agccggcata
     2641 gcctccatcg gaatgcatat taactttgcc ctgggcgcgg gtcttcccca ggccaacggg
     2701 ctcccggacc tctggtggat tatggcctcc tttgccatgt tcgcaggggc aggaagagcc
     2761 tttggtatag actattacct cattcccttt ttgaggaatc aattgagata cctgcagagc
     2821 aataagcggc ttaacatcct gaagggatgg gagttatagg attgtgggaa tgggaattgt
     2881 agcacaaaga ataatgggat ttatagcaca aaaagggatg ctgttcttat attgcagcat
     2941 cccttttaca tgttaatttt tattattttc ggcacacctt ctgtatgctt atttaattcc
     3001 gctatttgcg gcgggccttt tacatgcctg tttatttccg tcgttttcga tatcctgtct
     3061 atatgcttat ctattttccg ctattcttcc atgatgaatg tatcaaaaga attccgatga
     3121 tgatgataat tgcgggccat aatccattct ttacaagcct ccacaggacg ggaaacatcc
     3181 cgcttataag cgccattatc cccacaacta ccaggaccag gcccaggata gacattattt
     3241 tggtcctgtc taccggcggg aatacctctt cgctggcgac ggggtcgtaa tcttgttcgc
     3301 taaaaggctt ttccggtatt acaatagcgc ctattaaata gatgagaagc cccgatccga
     3361 aggtgggcaa cgtcagcacc gcccatataa gccttacaat cgtcacgtcg ataccaaaat
     3421 attctgcaat accgccgcat acgcccgcca gcatcctctg tttccttgaa cgatataatt
     3481 ttctattcat agttcccatc cccctgtatt taataaccca acgggtccca gcatttcttg
     3541 gcccattgtt ctctacgcag ttttatcata ggtttatacc ataattggtt taattcacac
     3601 ggttttgatc aacgcaccgg ataaatccta aagatttttt cgccctgcct tagtcaatta
     3661 taccacactt ttagcgcctg aggaccccca tgtttttgaa attagccatg aattttaaat
     3721 aatcgttgcc ccatgtgaaa agatggggta aaatgaggtt acaatcttaa ttgtttatga
     3781 aagagaggta tttctcatgg aagaggaaag agatatattg gtctttgtcg atgaggacgg
     3841 agaggaaatc gagatggagg ttttggacta tttctatcac gaggatcagg aatatgccct
     3901 gttaacaacc tatgacgagg attgccagtg cgaggactgc gattgtgagg aaaaagagct
     3961 ttacataatg aaggtcgttt tagacggaga tatagaagag tttgaacctg tggaagagga
     4021 gaggatggag tctctgatac aagccgtcga ggccctctac tacgaggacg atgaggaact
     4081 ttaacctttg tcaggcagca atgcaatgaa ctgaggcgat aggccaattt aaatagataa
     4141 tcccgtaagg atgccttata ccgccggcac caatattgct cgttatataa gatgatgtgg
     4201 ataatggata aggagtggat atgttatgca aagaaccgat ggtatgaact atgcacctaa
     4261 gggcaagcca aaaccagtct gcgaaaaggg ggattttgtc ttctctgcca tagccttgga
     4321 tcacggccac atctatggta tgtgcaacgg tctcaccgaa gccgggggac ttttaaaatc
     4381 cgtatatgac cctgaccccc agaaggttga ggctttttgc gaggcctttc ccggggttaa
     4441 ggttgcatcc agcgaagagg agattttagg cgataaggag gtcatgatgg tggccggcgc
     4501 tgctgtcccg tcagagcggg caccccttgg catgagggta atggatagcg gcaaggatta
     4561 ctttacggat aagacgccct tcaccaccct tgagcagctg gatttagcaa gagccaaggc
     4621 ggtaaagacc aataaaaaat atatggtata ttatagcgag cggctccata cagagagcgg
     4681 cgtttttgcc ggagagctca tcaaggacgg ggccattggc aaggtgattc aggtgctggg
     4741 tctggggccc caccgattaa acgccctcga caggccaggg tggtttttcg aaaaggagaa
     4801 atacggcggc atcctctgcg atataggaag ccatcaagcg gagcagtacc tatactatgc
     4861 aggtgaaaag gatgcaaccg tagtcaaaag ccaggttgcc aactacgccc acccccaata
     4921 cccggagcta gaggattttg gtgaagccat gatcctgggg gaaaacggga ccagcaacta
     4981 ttttagggtg gactggttta cccccgacgg cctgagatcc tggggtgacg gcagaacaac
     5041 tatcctcggg accaagggat atattgagct tagaaaatat atagatatcg cccggcataa
     5101 taccccggat catctgtttt tagtagatca agagggagag cattactttg actgtagcgg
     5161 caaggtggga tatcccttct tcggggaact aatcttagat tgcctaaacc ggactgaaaa
     5221 cgcaatgacc caggaacacg cctttaaggc cgcagaactt tgcctaaagg cacaagctga
     5281 tgccgtcaga ataagcggct aaaacataag aataagagaa aagcacaatg ggacggtcct
     5341 cctgtgtcat cggacacaga aggaccgtcc ccttgtgctg cctgcgttct tatattcccc
     5401 tagctaaaac atcttcctta agttttcgta tacctttgcg ccctcttttt tggctatctc
     5461 cttgccctct gcggcccaga tgaagcccct tcgcatgatc tctaaagcag gctttatatc
     5521 aaagatatcg gcatgatggc ctaaggcgtt atagaagacc cggcctgccc cccacctctt
     5581 ggtccagatc tggggtacgt ccacttcccc gttcgtggca tgggggccgt ccactacggg
     5641 aaatcttgtg gtagccaaaa cctctatgga aggatccaca tgcaggtagt attgttcact
     5701 ctttaccgga aaatcctcga tcccctcaac tatgggacta gagcccttct ttatgttgat
     5761 catgtattct accccatcgc ttccgggatg ggatacccag ttccctccgg tcataaattg
     5821 ccattgcaca cagtttctga aagcatcgca catgcctcca tggcatcccg caatacccac
     5881 tcctgatgcc actgcctcca cgacgggcca catctgatca tcggtaatct ggcccatggt
     5941 ccacaccgga acgatcagat ccagaccttt aagcttctcc ccatccaaaa acgaatccaa
     6001 ggtatcggac aattccacct gaaaccccga ttcttccagc aaacccttga aaatgtcgga
     6061 tacctgtacc ggctcatggc cgtcccagcc gccctgtact atcaaagcct ttttactcac
     6121 aatataacct ccttgagttt ttaatggatt tcctataact tcatccgtaa aacttcggtt
     6181 tattattcat agttggtttt taaaactccc ggctgaccaa attttcttga tgataccata
     6241 tttgacaatt atatatttgg tgatatccgg tacatcacat ctcccggaat attcccttaa
     6301 gccggggata aatatccgtg tagaccttgt atctctcatt atagagtgaa accaaatcgg
     6361 ggtccggctc taccgtaagc acggtcctta ccagcctttc agccgcttct tctacggagc
     6421 cgtattcccc acaccctacc gcggccagca tggccccgcc cagggccggt ccttcctccg
     6481 cttccaggat atccaccttc aggtttagta cattggccag aatcctcctc cataaggggc
     6541 tcttcgctcc gccgccgcac atctttgtcc tctcgaccct tatccccagg gccttggcaa
     6601 tctcgtagga atcccttatg gcaaaggcga ccccctccat catggcctgg gtcatatcct
     6661 ttctgccggt ttccatggtc atacctataa aggcaccctt tgcgtttgga tcattgtgag
     6721 gggaacgctc ccccatcaaa tagggcagaa agtatatcga atttttgccc aggttttcta
     6781 tgccttcttg ttccccgtga aagtcctcgg tatctaggat atcttccatc caccacttgt
     6841 tacaggaagc ggcgcttagg acgaccccca taaggtgata gtttccgtcg gcatgggcaa
     6901 aggcatggag ggcgttgttc tcgtccactc cgaacctttc gctggagata aatatagtgc
     6961 cggaggttcc cagggaaacg ttgcattttc cctccccgac ggtcccggtc cccacagccg
     7021 cagcggcgtt gtcaccggcc ccggccacga cctttacgtc cttgttaagg cccaattcct
     7081 ccgcggtttc tcccttaagg gtccccacaa cctcatagct ttcaaaaatg tcgggcagct
     7141 gctcccgggt taccccgcag atttccatca tttcctctga ccagcgtcta ttttcaacgt
     7201 caaacaacag ggttcccgat gcatcggaag ggtccgtaca atgaaccccc gtcagtctgt
     7261 atgctatata gtccttgggc agcatgatct tatgtatccg cttatagttt tccggctcat
     7321 gctttttcat ccacagtatc ttgggcgccg taaaaccggc aaaggcaata ttggccgtat
     7381 atttagacag cctttccttc ccgattttct cgtttaagta atcggtctcc tccgcagttc
     7441 tcccgtcgtt ccagagaatg gtaggcctta taaccctgtc ctcctcgtcc agaaccacca
     7501 gcccgtgcat ctgcccgcca aatccgatcc cggcgacccg agatttgtct ataccgctga
     7561 ggagttctga aagtccctcc agagtatttt cataccagtc ttcggggttc tgctcggacc
     7621 agcccggcct gggaaagctg atgggatatt ccttagatac gctctttaaa atagttccgt
     7681 ccccggacat cagcaatagc tttaccgccg acgtcccaag gtcaattcca atatagtaca
     7741 tgttacacct cttttccgat agactaatgt gtagccgaat ctaacaggat aatcagctta
     7801 aggcgtcctg cttggtcggc gaactaacac aacgcgttaa tttatgaatc agccaaagag
     7861 tatgctgttt ataacgccct ctaagtactc ctgtctgcca gacccgggca attccggagc
     7921 ccccaacttc tcggcatact cagccagctc taccaagctg gtctccccgt ttttaatctt
     7981 ggcgcctatc ccgctgtcaa agcttgagta cctctcctta acaaaatcgt cgatcctgcc
     8041 gtcctctatt atctgtgcgg cctttataag acccagggca aaggtatcca tccccaggat
     8101 aaacccatgg aacatatcct cgtgggtata gctgggcctg cggtttttgg catcaaagtt
     8161 gaacccaccg ttttcaaggc ctccggcctt taaaacctcg tacatgcaca gggttgcctc
     8221 gtatacatcg aaggggaatt ggtccgtatc ccagccaaga agataatcgc cctgattggc
     8281 gtcaatgctt cccagcatcc cgtttatggc gctgaccctg agttcatgct ggaaggtatg
     8341 tcccgccagg gtcgcatggt ttgcttctat gttcagcttg aagtccttat ccaagccata
     8401 ttcctttagg aaaccgatgg cggtagccgc atcgaaatcg tactggtgct tggtgggctc
     8461 tttgggcttt ggttcaataa agaaatcccc ctcaaagcct atgctgcggc cataatccac
     8521 tgccatcttc ataagtttgg ctatattgtc cagttcaaac ttcatgtcgg tgtttaaaag
     8581 ggtctcatag ccctctctgc cgccccagaa tacgtagccc tttccgccta atttgacggt
     8641 caaatccaga gcttttttaa cctgggctgc tgcaaagcag tatacatcag ccgaattggt
     8701 actacctgca ccgttcataa acctggggtt ggtaaacatg ttggctgtgc cccacagaag
     8761 ccttttaccc gtggcatcca tcttttcctt tatgtaatcg gtgatctcat ccaggcgagc
     8821 attggtctcg gctatggttg cggcctccgg taccaaatcc gcatcatgaa agcaaaaata
     8881 ctctatgccc agcttctcca taaattcgaa tccggcatcc accttggccc ttgcatgctc
     8941 catggtgtca acctgggcac caaagctctt atcggcggtt cctaccccga acatatccgc
     9001 accggttgcg gagaggttgt gccaccatgc catggcaaag ggcagatgct ccttcatggt
     9061 cttacctaaa atcactttgt cttcatcata gtacttaaat gccaaggggt ttttgcttga
     9121 cggcccctca tattttatct ttggaatctc attaaaaatc tcagacatgc taatcctcct
     9181 cgttatatta tgatcatatc cctattatac actaaatttg gacattacac atactttaat
     9241 cccctgtccc ggtccattat ctgtaggtaa tggaccggga cagggcatat gactttggcg
     9301 gtttattcta aatccatgac gaagttaacc tttagtagct atggatagac ctttaatagc
     9361 gatagataga cctaaccgct aaagctcatt aaaaattcga actgatgctt atcggccaag
     9421 accttgtgct ttggatccag ctgggtggac cacgccatat ccccgccgat acccgccatg
     9481 gccccgtcga tgtttatata gacctcgtct ctcctcacaa gctcatgctc gtgatagtta
     9541 ttccaataat cctctacggt gttgtgatgc acatcaaagg agaaggagga acccttaacg
     9601 gtaatcctgc tgccgtcctc atcctccagg gtaaaccacc tagtatcggc gtggctgcca
     9661 ttgtgggaaa cgggcacgaa gggaaaatgg gtgtcctcaa ccgacgagct gtatttaccc
     9721 acgggagccg cgagctttct gtcgcaatag ctctcgccgg gccctcttcc gtaccagtgc
     9781 agggtattaa acccttccgg cgcaataaag ccaaggccga ccctcggaag atggacgtaa
     9841 ttgctgtcta tatctatggc tgcgcttaca ccgatatccc cgtccctgta gacggtgtag
     9901 gatacccggc tgtatatgct gcccttgtcg ccgtcaatcc tgttggtaaa cgcgattctg
     9961 gcgctgcctt ccctttggct gatcccgtag tccatagcca agggatttct cttcaatttg
    10021 ccgggcataa aggaaggcca taaatcatcg gattcccccc accatttgga ttctaagtga
    10081 agacctgtcc tgctcctatc gaagttttct accccgctcc ttaagatgta ctccttaccc
    10141 tttcgcatat agctgtgtag ggtattctcc tccagatcaa agatcacctt aaaatcctgt
    10201 ccctttaccg ttaaaaggtt atccgcctct tctatggaca gatccccgcc atctgcggac
    10261 attttcggca ccatgggcgc cgggcctttt atttcaaact ggtagttggc tatctcatga
    10321 ccctttttgg cccagagata atcatccgca gtctttaccc ttatatttat atatacctca
    10381 tcggtttcat cctctacagg ggacaggtcc accctaaagg caatgtcccc gcatggtcca
    10441 tccgctatag gaagatgaat gtccccgccg tctatcctct caccgtgtac caggatttca
    10501 tattcaagag tcaactcatc aaagcccatg ctctgggtcc tatttcttgc tatatagcct
    10561 ccgcaaaccc cttcgtcggg ttctatgatg atgggggatt gggcctgctt cagttcaagg
    10621 ccgctgggtt ttggggtaag gtccgccatt acgatcccgt ttgcgcacat ataataggga
    10681 cacacccaat ccgttagatc ctcgccccaa tctccgccaa atccaaagaa ggtctcccca
    10741 tcttcgttga cggcgggcag gcatttgtcc tgccaatccc atacaaaacc gccctgaaag
    10801 atctcatatt tctcagccag gcggttgaac tgttcaaaat gcccgctgga attggttatc
    10861 tggtaagcat attctattag gactatgggc cttctgtcgt tgttgtcggc aatcatatag
    10921 tccaggagct ccatggtggg atacatggta cattttatat cgctggcgat tagccccgga
    10981 tcgttgtttt cgtattgtac caatctggta tcgtcgtact ctctaatcca gttcgccatg
    11041 gccgcgtgtc ccgccccgta tccggattcg tttcctaagg accaggatac gattgaaggg
    11101 tggtttttat gtatcagaac catacgtctg gcacgctcta gcatagcctc cgcccattcc
    11161 gggttgttgg tcagattgcc tccaagcccg tgggtctcta agttggtttc gcagacaacc
    11221 atgaggccat atttatcgca taggtcatac catataggat catcgggata gtggctggtc
    11281 cttacggagt taaagttaag ccttttcatg agcttgatct cctctatcat atgctccctg
    11341 ctcaccgttc ttcccgtcaa ccaaaaatgc tcgtgcctgt ttgtgcccct aaagactatt
    11401 ctttttccgt ttagcttgat gacgttgttt tcgatggata cctctctgaa tccaatgttt
    11461 agggcctcaa aatccttctc atccccgacc ccgtcaatca aggtaaatac aaccctgtac
    11521 aaattcggcc tgtcaaaatc ccaggggagt acgtcgtcaa cctcaagttc gaagaaagcg
    11581 gactcgctta taggccgcat ccccttaaag ccccacccgg agcccatgcc cataatgggg
    11641 cttgtggtat caacgggctt tgtgcgctct gcaagtagcc tgcccttttc gtcaaagagg
    11701 gagagcctta cagaagaatc cgcgaagccc tccgttctat ttacaaagca gtgggcgttt
    11761 ataatgccgc caccgtttcc gcagggtctt gcgtccacct taaagtcctg gatgcgaagc
    11821 acaggctttg cgattaggtt aaccgatctg aatatcccgg acagatgaaa gtagtcctgc
    11881 ccttccagcc aagtaccgtc acagaacctt agtaccgcca gggttataat gttttcacca
    11941 gaatttagat actccgttat ctcaaattcc gacgggagct tgctgtcctg ggaatagcct
    12001 accggatgtc cgttaatcca aagataatac gcggactcca ctcccccgaa gtttataaat
    12061 acgcttctgt ccccaaagga ttccggcagg gtaaaggatc ttctgtagat gccgcaggca
    12121 ttctcctccg gtacatatgg gggattatat ctttggtaga tatcataatc cttctttttg
    12181 cctatatcaa gtaagtactt ctccccaacc ttatccttaa agggatagag ggtatttaca
    12241 tatactggtt tgccgtagcc ttgaagttcc cagtttcctg ggacctttat cttgccccat
    12301 tccgatagtt cggcatctgg ttgattgaac ccggccggca tggactctgg attgtccacc
    12361 agtaaaaagt cccattcccc gtctaaggaa aatgtgaact tagaatctcc gcccagcctt
    12421 gcctgatctg cgctttcata tgcggaccaa ggaaagtggg caggtgccct gtttatttgg
    12481 gttatgtatt ggttatgata atccggtttc ttatgaaaac ctttaaacgc ttccagcatt
    12541 atattgcctc cttaaaaaag gggcggcttc ccatccgtta gaagcaggag gagccgtccc
    12601 tttcgctcta gtttttattt taatctactt tgaattctcc ccgatatagt gcccctatat
    12661 ccgtcagcat ctggtgaacc atgggtttat ttaccgcaaa ttcggactca ccgtctataa
    12721 acaccgcgta aaagtcgttg tcatagggca cgtagtttat aagcatctgt cggtttttcc
    12781 ccttgttaag gtggaaggtg gccttaattt ccggccttcc tgcggcaccc ggcttgacct
    12841 ccgaatccgc cagaacgccg acgacgctct gatacacctt tttaaagggt ttgtcttcga
    12901 cttcttttcc atctaccctg taggtggtta ctacctcgtc ctcttcgtcc tcttcttctg
    12961 ccggttttgt ctcccgttcc aaggtcaggg tatgcttttg ccccaggccc tccacttcga
    13021 tcttatccac attatcgata cttacgatat agataaattt atccaccagc tcaaaggccg
    13081 ttacatccaa gaacttcgta tttgccttct tcatcccata gacggaattc gaatccggca
    13141 atttaaagta taccatcccg tcgtcatact catccccaat cagtaggtgt atgctgtttt
    13201 catcgtcctt aatgataaat tccgcctttg gctcatcaag gccgtacttt gaaagatcct
    13261 ctgccccatc ttctacaaaa tcttcgatgg acagcatagg catagcctct aaatacttag
    13321 acaccgcctc gccgtttacg ttcatagttt ccctgtatgg ccttatcatc tgccacagcc
    13381 caaggttaac ctgaacagca tcctcttcat cttcctcgct tagcctgatt tcgatgggcc
    13441 tgtcgtcctt cttgtccacc agaagatagc tcagcgcctc gggattaacc tggggcaggc
    13501 tcttgtccct gacatcggcg agggtatacc ttaggtgatc cccgtggttt gtccagacct
    13561 catagacctt cgggtcccca tctagcataa ggtaataggt gttgcctatg ggggtttggt
    13621 tcccgagata gaggatattt ttagaaccgt cctttaaggt aaccgtaccc gtagcttggg
    13681 gctcctttag cccgtaaggc gcaagatcgt ccggggtctc ctcctctata atacgttccg
    13741 cttggaggtt agcaaagcta tatgctaaat cgtccacccg catctccttt aaaaggattt
    13801 catgggggta atccacttcc catacggact tcttttcctt ctccttttta ccgtcctttt
    13861 cgacttcgac ttccttctct cccctggtaa aggtcaattc gccgaattcg tttttaatct
    13921 ccatcttttg gatttcatcc ttctcgagct ttaggacaga aatcttttcg acctcttccg
    13981 gaccctcggg ttcgggccta ttggcaagga aaaaatacga ccccgccaat accccaagca
    14041 ctaccaatac tatgataaga tttctggact tcctcatagg tgtctcctcc taagccacat
    14101 taccagcccc gcacccagaa taagcagggg tattaaaatt acggcaatgc cggcaaacaa
    14161 taatcgctgg aaggcattaa tgttaaggta ttggcttagc atgctcttag gtctaatgga
    14221 gatgctctcc tgtttatcct ggagccagtt taggctgttt agaacaaaat ccgcatttcc
    14281 gatgctggcg aacctgggat ctataaactc tgcgtctccc attaccacca gctttgtctc
    14341 gtcgtactta ccgacctttt cgtcccaggt cttatcggct atggctacgg cgatattaaa
    14401 gggtccatct aaatccccct cttccttttc aggggcggtg gatttcatat cggcccttcc
    14461 ccaggcatcg ttgctggtta taagcagggg ttcgatctct atgcttctct ttttcatatc
    14521 cagctctatg ataccctggg cgcccatggc cagtacctgc atcttattag ccttcattgg
    14581 gcttatgatc ccatggcttt gcatattggg cactatccat gcaggatgtt gaaagtaatg
    14641 gtttttatcc ccttctacgg ttaatgcctt gtttaaggct acaccgtagc tctttaaaag
    14701 ggcatcaaag ttgggcatct cgtcttccac gatatccatc aaaaataatg cgcgtccctt
    14761 gttctccaaa tattccctga ccttctcttc ctcttcaccg gtcatatccc tctggggggc
    14821 tattatcatc agcacggatg catcttccgg tacctggtcc tccgtcagaa gggacagggt
    14881 cttaatctcg tagttctcaa gctccatctg ctttctcagc tcgtagggca gggcctcttc
    14941 catatgcccc tctaaggtat atactacggg gagctcctcc gccgtcacat attggatagc
    15001 gctggttacc ctctcctcca tggccaagga ttcggcacgg gtgtttccgt actggtcggt
    15061 ctttacgtta aagaggtcgt agtagtctat aaccttatac ttatcctcgg tctctacgat
    15121 gtagctgccg tctcttaggc tggtaccgtc cttggtatac ttgctggaaa aggtggggtt
    15181 ttttacggga tccacatagg atatggagat gtttttgttc ccccgcctat acctttgcag
    15241 tatctcgtca aacaccttgt cctcattacc cgtctggtaa agaccgatga tctctatctt
    15301 ttgatccaga ttctttagta tcgtgtccgt ctgatccgac agggagaaca atctattctc
    15361 agtcaaatcc actctgaagg ggatctgtcc taccaccaag tttattataa ccaggatagc
    15421 cagtacaaat gccgtcatca aagtcgcata tccgccgtat ttaaactttt tattcttgaa
    15481 tgaatccaag atcttgattc tatttaattt cttcaccctg actcccccct aactccatcg
    15541 tcttttctcc agcaccctaa cggtcatata gacgaaggct gctgaaaagg taaggtagta
    15601 tactatggga cctagactca aaatgcccat ttgaaattct tcgtaacgct ttaacagcga
    15661 aaaccattca aaaaccctga cgataaatcc gtcaaagaag tatctgtccg ccagaaaata
    15721 ggttagtact atagccgccg cacccactaa ggcggtggct acgccaacgt atatgtttct
    15781 ggtgttaaag tatacgaatg ctcctaaggc cagggcaata atacctgcga agataattcc
    15841 ggatgtccgg tccgtgggaa gtccctggac tacccaatcg attatccaca taaataatag
    15901 ggcgctgaag gtgattaccg cagcaattac ctggttatcg gtaaatgtgg atactaagag
    15961 gcctatggca atgaaagccg atcccaagag caaaaatcct atatacccgc ctactatctc
    16021 ccatattgcc aggtttccga aaaatgacag gatgatggga tagacacagg ttataagaag
    16081 ggtaaggaca aacaccgaaa ccgcggctag atattttccc agtactattc cggtcaagga
    16141 aagcggactg gtaaacaaca gctggtcggt cctctgcctc ttgtcatcgg gcatcagcct
    16201 catggtcagg ataggcacca ctatcaggaa gataaaggtg atgctgccca gtaccgaatt
    16261 atagtttggg ctggccggga acaagttggt catggcgaaa aagaatcccg acaatataag
    16321 gaaaaatccc ataaatataa agcccgtaga tgaagtaaaa taggctctca attcgcgttt
    16381 atatatagct aacatattag gacacctcca ctttttcctg ggtggttacc tgtaggaaaa
    16441 tatcctctag agtaagatcc atggatttca tctgtaggat agggtacccg gcacggctta
    16501 aggcgttaaa caacggtctt ctcacgtcaa tatccttttc cgcctctacc cacacgtcta
    16561 tggtatccgg ttccataatc ccctgggctt ctacgtactt tacgccggcc acatccttta
    16621 acacctttac agcctgcctt tcggaagcgg caatcctgag gctcatctta ctgctgtccc
    16681 caagcctttt agatagatta tccggcgtat cgctggcaac aatcctgccc ttgttgatga
    16741 tgaccagacg ctgacacacg gcgctgacct ccgggagtat atgagagctc aatatgatgg
    16801 tatgcttctt accaagctgc tttattaggt ttctgatctc aattatctgc ttgggatcaa
    16861 ggcccaccgt aggctcgtcc aataccaaaa cctggggatt gcccaccaat gcctgggcca
    16921 ggcctaccct ctgtttgtac cctttggata ggttcttgat aagccggtcc ctaacatcag
    16981 ttattctcac cagctctaaa atcctgtcca tatcctgttt tttgtccttg tcatcaacct
    17041 tttttatttc gaatacaaaa tccagatatt ctctgacggt catatccaca tataggggcg
    17101 gaaactccgg catatagcct atgcgtttct ttacctcttc gggattgtct aagatgtcat
    17161 atccgtccac tgtgacggtg ccttctgtag cagaaatata gcccgtgata atgttcatgg
    17221 tagtggactt acccgccccg ttgggcccta gaaagcccaa tatttcgcct tcgtttacgg
    17281 taaaatccaa attatccacg gctaaatgct gcccaaaccg tttagttacg ttttgaatct
    17341 ctatcaatta ttctcccccc atttccgcga tctaaggaat actaaacaca catgattata
    17401 tcatcactgc tgattatagc aaagttattt aaataaaata tgaataaagt gttaacaatg
    17461 gagttgagat gggcttatct ttatggtagg cttgtaccat gtctcttttt ggtcggtccg
    17521 ttattatttt attattgtcc tgcctaatta ggttgaataa gaagggatgg atcggcattg
    17581 ttttaatacc ggataagatt gcactaaatt ccagaaaaat gtgcactttt acaccggaat
    17641 acgcaccgac ttgggggttt gaatgaatta tgttaaagat aggctcgcat ctgtccgtag
    17701 ctaaaggttt tgaagccatg ggtctggatg ccataaagat tggggctaat acctttcagt
    17761 tctttaccag aaacccaagg ggtagtaaag caaaggatat agatcccgaa gatgcccaaa
    17821 aactaaaaga tatcatgagg gagaatgatt ttgcaaagat ccttgcccat gcgccctaca
    17881 cattaaatcc tgcatccaac aaggaaaatg tcagagaatt tgcaaggctt attatggaag
    17941 acgatcttag aaggatggag tatttcccca ataatctgta caacttccat cccgggagcc
    18001 acgttggcca gggtgtacct aaggggatag aatacatcac taagctgcta aatgagatct
    18061 taaaacctga acatacgacc acggtactgc tggaaacaat ggccggaaag ggaaccgaga
    18121 ttggtaggaa ttttgaagag ctgaatgcaa taattgatgg ggtaaaatta tctgaaaagg
    18181 ttggcgtatg ccttgatacg tgccatgtat atgacggagg atatgatata gtaaacgatt
    18241 tagacggggt attagaagag tttgataggg ttataggtct taataggctc cacgctatac
    18301 atataaacga cagcaagaat cctttcaaaa gccataagga taggcatgaa aagttagggc
    18361 aaggtacatt ggggttagag accatagtaa gaatcatcaa ccaccccatg ctcaggcatt
    18421 tgccctttta tctagaaaca ccgaatgagc tggaaggcta tgcggaagag attagggtgc
    18481 tgaggaggag ttatagtccc tgattgctcc cagtcccctc attgtagatt aatagcccca
    18541 attacacgta attatttacg cccccctaaa tctatcagca tgataattgc acagatccat
    18601 tataattatc atgctgataa atttagtttc ccgtattgtt tatctatagc taatattcca
    18661 accagctacc aaataaaaat tacgctgtcc ataccccttt attatctatc ctatatttgc
    18721 ctatagtagt gttaactgcc atagaaccgt ctggatacag ataataccat ttgccaccag
    18781 ataataacca acctatttgc attattccac ttttgtccat gtaataccac ttaccatcga
    18841 ctttttgcca acccgttgtc atagcaccac ttgcgcccat gtagtaccac ttaccgccaa
    18901 ccttttgcca acctgttgtc atagcgccgc ttgcgcttat atagtaccac ttaccgccaa
    18961 ccttttgcca acccgttgtc atagcgccgc ttgcattcat atagtaccac ttgccgccaa
    19021 ccttctgcca acccgttgtc atagcaccgc ttgaatccat gtagtaccac ttgccgccaa
    19081 cgggcaccca acctgattgc atcactccgc ttttgttaaa gtaataccat ttgccgcccg
    19141 attttaacca tccggtctgc ataatcccag ttttctcatc cataaagtac cacttgtctg
    19201 cgtctaacat ccagccggtt accattttcc cgtcttcact tagataatac cactcaccgt
    19261 tgctgttctt tttccaacct tggtccccat cggggtcatc ggacaagaaa ttcttgctat
    19321 atgcctctat tggcacacct gaataacttt gtacggtgtt gggaatatca atgctcatga
    19381 tgtcgtttat gctctgtttc gaatcaaatt tcaaaacgaa ggtttctgta tatacagtac
    19441 cattttctga cgtttcctct tcactatatt gaattttgta acctatcttg ccacctgtct
    19501 tgtcctttat taaaatacca ttaaccgttg caggatcaat atatttatca aatactatat
    19561 ctatccgatc agcgaacaca ttaagacttt ctaacttagg agcagttatt gatatcatat
    19621 ctatatttac atctgtttgg gggggcggta ccggcaacca attgctgtat atagtttcat
    19681 agccatcaag ttcatattgc acctgccaca acccctcggg tacgtcccat gcatagttac
    19741 catcctcatc ggtatatag
//
LOCUS       DASRNX010000159         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_775125,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000159 DASRNX010000000
VERSION     DASRNX010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775125"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1104
                     /locus_tag="VFD89_06385"
     CDS             256..1104
                     /locus_tag="VFD89_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57854.1"
                     /translation="MTTKLIIIEGLPGLGKSTTARMVYDILKSKGIKSELFSEGDYNH
                     PADYDGVAYFSDGDFNKLKNMHLENENILNKIKIRHGSGYLIPYRKIIEEKKISFPDR
                     LLSDITKNNVYELPLDLHTVLILNRWEDFVNRCVHEDKTVVFECCFIQNPVTMTMIKN
                     NSTPQVTIDYVQNLAKMVVPLKPILLFLEQKNIEMSLKKAISERPKEWFESFEDYYTN
                     QGYGLDNNLKGLDGVISILKSRYRLEQEIYESLSLTKYRIDNSAFDLNLHRETIGSIL
                     EPHLPL"
     gene            1192..1383
                     /locus_tag="VFD89_06390"
                     /pseudo
     CDS             1192..1383
                     /locus_tag="VFD89_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1569..2345
                     /locus_tag="VFD89_06395"
     CDS             1569..2345
                     /locus_tag="VFD89_06395"
                     /inference="COORDINATES: protein motif:HMM:NF015081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZJ57855.1"
                     /translation="MKVYRSKKAGRMLSKTYDCLLDQWKVGFKQIDVETRYGLTHVIE
                     CGDKKADPLILFHGVGDDSALMWIYNIGALAEHFHVFAIDTMGGPGKSRPNSSYNKGF
                     DQVLWMDDTLDSLNIESGYMAGVSNGGYMVQRYTIERPNRVRKSVCMSSSIFDEISEN
                     PMKTMMKVFLPEALFPTDKNVVKLIKKLSGAHSKVFTENDLKTSEAIALAIATHRFQR
                     DGRFIPTACFTNRNSPLIETGDILLTRYNGTLQMVAQGVQ"
     gene            2347..2583
                     /locus_tag="VFD89_06400"
     CDS             2347..2583
                     /locus_tag="VFD89_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57856.1"
                     /translation="MAMRFHRIQMFSPDQIRLLVGKTMFICGGMDPMGDTSKAVGKFK
                     EYGIIYRIFEDAGHGVNHECADKVNNLLITYFRE"
     gene            2715..3125
                     /locus_tag="VFD89_06405"
     CDS             2715..3125
                     /locus_tag="VFD89_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018883054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZJ57857.1"
                     /translation="MYISINYAGNDDLDYLAQNDIHISREMIEKKIGDREIIAARVDG
                     KLVGWLRYNYFWDNTPFMNMLYLEGDFRKRGIGSKVVLYWEKVMKEQGYNMVMTSTQA
                     DEEGQFFYRENGYRDAGCLLLENQALEILFKKTI"
     gene            3199..>4483
                     /locus_tag="VFD89_06410"
     CDS             3199..>4483
                     /locus_tag="VFD89_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HZJ57858.1"
                     /translation="MFNKEGQRLIFSYDSEKLWIEPWGDNALRIRATKMAEMPKEDWA
                     LLSHDNDSMADISITPYGATIVNGKIKAELNSAGKITFYNQKGELLLEEYVRNRREYT
                     FQPNTKPTGFASALEIEAREFKPILGGDYSITVRFESTREEKIFGMGQYQQPFLNLKG
                     CDLELAQRNSQASVPFMISNLGYGLLWNNPAIGRVTFGKNLTTWQSHSSKVIDYWICA
                     GDSPSQIAETYGEAVGTVPMMPDFAMGFWQCKLRYQTQEELLQVAREYKRRGLPISVI
                     VIDFFHWTMQGEWKFDLDYWPDPDAMIEELKEMGIELMISVWPTVDHRSENYKEMVEK
                     GYLIRTDRGHRIAMDFHGNTAHFDPTNPEAREYVWKKIKKSYYDRGIKIFWLDEAEPE
                     YTYYDFDLYRYHMGPNMQIGNIFPVMYAKAFFDGME"
BASE COUNT         1417 a          748 c         1115 g         1203 t
ORIGIN      
        1 gtctcgatgc agctactgat ggatttgatt atgtggaagc ctatgtaaat gaaatttata
       61 caaaacttga tttcagaggt gcagtggcga tgtacgaaaa atgcggattt accaaatttg
      121 ccgagcgtgg gggtaaggcg gttatgcgga agggactgaa ataaattgat gttgatttca
      181 tggtaatatc agggcctatc ctcggggcct ttgtttggaa atatataaac atatgtgaag
      241 cggagcggaa agaaaatgac aaccaagcta attattattg agggactacc aggattaggg
      301 aaatctacga cagcccgaat ggtatacgat attctcaaaa gcaaaggcat taaatcagaa
      361 ctttttagtg agggtgatta caatcatcca gctgattatg atggagtagc ctactttagt
      421 gatggggatt tcaataaatt gaaaaacatg catttggaga acgaaaatat cctaaacaaa
      481 ataaagatta gacatggcag cggatatttg atcccataca gaaaaataat tgaggagaaa
      541 aaaatatcct tccccgacag gttgctaagt gatataacta agaataatgt atatgaatta
      601 ccacttgatt tacacacggt gttaatattg aatcgttggg aagactttgt taaccgttgc
      661 gttcatgagg ataaaactgt tgtttttgaa tgctgtttca tccaaaatcc cgtgactatg
      721 actatgatca aaaacaattc taccccacag gtcacaatag actatgtaca gaatctggca
      781 aaaatggtgg tcccgttgaa acctatcctt ttatttttgg aacaaaaaaa cattgaaatg
      841 tcattaaaga aggctatcag cgaaagacca aaagagtggt tcgaaagttt tgaggattat
      901 tatacaaatc agggctatgg tttggacaat aatctgaagg ggttagacgg agtaataagc
      961 attctaaaat caagatacag acttgagcag gaaatttatg agtctctttc gttaaccaaa
     1021 tatagaattg acaactctgc tttcgactta aacttgcata gggagacaat tggttccatt
     1081 ttagaacccc acctgccctt gtaagcattc aagatgggga tataatgtgg gatctaggtg
     1141 tattccgtag taatgttttt gtcatttaat ttattttcag gaggtaggaa tatgaccata
     1201 ttcgagaaaa cccgtatgtt cgtctatcgt aactcacgtc ccttggatat tgctaggtgg
     1261 caatatcatt ttgaggatgg aagtaaagaa gctgtactaa ccgcccttgc tccttatcag
     1321 aacgaggatg gcgggtttgg tcatgcactt gagccagatt cgtggaaccc aaattctacc
     1381 ccgaggaatg ggccatcagc aaaaactggt ggaagggtag cgatgcggta ttgaatattc
     1441 tatacttaaa ggggatggga agattatgat aggcaacccg tttcaataaa agaacgcttt
     1501 ttcttgtggc gggacgatat aggggacaag ttgtacaggt tatctttaat ttttgggggg
     1561 gtgtgaacat gaaggtatat cgaagcaaaa aggcaggcag gatgttatcg aagacatatg
     1621 attgtctatt agaccaatgg aaagttgggt tcaaacagat cgatgtagaa acccgttacg
     1681 gattaaccca tgttatcgaa tgtggggata agaaggcaga tccattgata ctttttcatg
     1741 gtgtaggtga cgattcagcg ctaatgtgga tttacaatat tggggcctta gccgagcatt
     1801 ttcatgtttt cgctatagac actatgggcg gaccagggaa aagcaggcca aatagttctt
     1861 ataataaagg ctttgatcaa gtcctgtgga tggatgacac attggactcg ttaaatattg
     1921 aaagtggata tatggcaggg gtgtcaaatg gaggatatat ggttcagcga tataccatag
     1981 agagaccgaa tcgggttaga aagagtgttt gtatgtccag ctccatattt gacgagataa
     2041 gcgagaatcc gatgaaaacc atgatgaagg tctttctgcc cgaggcatta tttcctacag
     2101 ataagaatgt ggtcaagctt attaaaaagt taagtggggc acatagcaaa gtatttacgg
     2161 aaaatgatct taaaacatcg gaagcaattg cattggcaat tgcaacacac aggtttcaac
     2221 gagacgggag atttataccc accgcctgtt ttactaatcg gaattcaccg cttatagaaa
     2281 cgggagacat cttactgact cgttataatg gaacattaca aatggttgct caaggggttc
     2341 aataatatgg caatgagatt tcataggatc caaatgttct caccggatca gatcagacta
     2401 ttggttggaa aaactatgtt catttgcggg gggatggacc cgatggggga taccagtaaa
     2461 gcggttggaa aattcaagga atatggtata atatatagga tctttgagga tgcagggcac
     2521 ggagtaaacc atgaatgtgc agataaggtt aataatttgc ttatcacata tttcagagaa
     2581 taatttttct gatgtagtgc ttgctcaagc tggactggtc aggaatatcc ggtgccttct
     2641 agaattattc aggactcgtc gtattgctaa ggaccgggac tggccttaga acaggatagg
     2701 tgtgaggtga atttatgtat atttctataa actatgccgg aaatgacgat ttggattatc
     2761 tcgcccaaaa tgatatacat ataagcaggg aaatgattga aaaaaagata ggggataggg
     2821 agataatcgc agcccgtgta gacgggaaat tagtcggatg gctgagatac aattatttct
     2881 gggacaatac accgttcatg aatatgctct atctagaagg ggattttagg aagcggggga
     2941 taggttcaaa agttgtgctc tattgggaaa aagttatgaa ggaacaaggg tataatatgg
     3001 ttatgacatc cacgcaggca gatgaggaag ggcagttctt ttacagggaa aatggctata
     3061 gggatgccgg gtgcttattg cttgaaaatc aagctctgga aatccttttt aaaaagacca
     3121 tataggggga gctatacaga tatatcagaa attgcccctt gcaaaaaaaa gtttttacaa
     3181 tacgacagga ggaattatat gtttaacaag gaaggacaac ggctgatttt tagctatgat
     3241 tcagaaaagc tatggataga accctgggga gataatgccc tcaggataag ggctacgaag
     3301 atggcagaga tgccgaagga agattgggct ttgttaagcc atgataatga tagtatggct
     3361 gacatttcca ttacaccata tggggcgacc atagtgaacg gaaagatcaa agcagaattg
     3421 aacagtgccg gtaagatcac cttctataat caaaagggag agctattgct ggaggagtat
     3481 gtaagaaaca gaagggaata cacttttcaa ccgaatacaa aaccaacggg gtttgcttct
     3541 gcccttgaga tagaggcccg ggaatttaag cccatcctag gaggggacta ttccatcacc
     3601 gtacgctttg aatctacccg ggaagaaaag atatttggga tgggtcaata ccaacaaccc
     3661 tttcttaatc tcaaggggtg cgatttggag ttggcccagc gtaactccca ggccagtgta
     3721 ccgtttatga tctccaatct cggctatgga cttctatgga acaatccggc aattggccgg
     3781 gtcacctttg gaaaaaatct gactacatgg cagtcccatt catcaaaggt gatcgattat
     3841 tggatttgcg ccggtgatag cccctcgcaa atagcggaga cctacgggga agccgtcggt
     3901 acggtcccta tgatgccgga tttcgctatg ggattctggc agtgtaagct gcgctaccag
     3961 acccaggagg aacttttgca ggtggcccgt gaatataaaa gacgcggatt gcctatatcc
     4021 gtaattgtaa ttgacttttt ccattggacc atgcagggtg aatggaagtt tgaccttgat
     4081 tattggccgg atccggatgc tatgatagag gaattaaagg agatgggaat tgagctcatg
     4141 atttccgttt ggcctacagt tgatcacagg agtgaaaact ataaggagat ggtggaaaag
     4201 ggctatctca taagaaccga caggggacat agaatagcta tggacttcca tggaaatacg
     4261 gcccatttcg atcctaccaa tcccgaagca agggaatatg tatggaagaa gatcaagaaa
     4321 agctattatg ataggggaat aaagattttt tggctcgacg aggcggagcc cgagtacaca
     4381 tactacgatt ttgacctgta tagatatcac atgggaccta atatgcagat aggaaatatc
     4441 tttccggtga tgtatgccaa ggcctttttt gacggtatgg aag
//
LOCUS       DASRNX010000160         5461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_247253,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000160 DASRNX010000000
VERSION     DASRNX010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247253"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFD89_06415"
     CDS             <1..616
                     /locus_tag="VFD89_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZJ57859.1"
                     /translation="RWGTFAKVMQTTYMMPYFLSYAVVAIVANAFLHRDNGLINSLIR
                     MSGGVGKTNWYHQLRLWPPLLVFVNAWKGVGYQTVLYLAVISGISVEYYEAAVLDGAS
                     KMQQARYITIPHLRYIVGISLILAMSSIFRGDFGLFYNVPMNQGMIYPVTDVIDTYIY
                     RALIYLNNVGMSTAAGLYQSVVGFILVIIVNHIVNRIDPESAMF"
     gene            652..1575
                     /locus_tag="VFD89_06420"
     CDS             652..1575
                     /locus_tag="VFD89_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZJ57860.1"
                     /translation="MAKEEKHINKLNSISEGWNMVFVIILLIIALLVIIPMVLIIVIS
                     FSSEISIADKGFSFIPTQWSISGYKYLLKTGDQLLDSYIITIIYSVTGTFMSLAVMTM
                     YAYVISQRNFKFSRLFTWLLFFTMLFGGGLVPSYILNVRYLHINNTIWIFLLPSLVNA
                     YYTIILRTFIRTTIPDSLFDAARIDGAGHFRIFISIVLPLFKTGIATVGLFGLVSRWN
                     DWFIGMLYIQNPKLIPLQTLLTKLQDSIKFLKENAAIAGTPDGIALLRALPQESLRMA
                     CTVAVILPILFAYPFFQRYFVQGLTIGSIKG"
     gene            1712..3343
                     /locus_tag="VFD89_06425"
     CDS             1712..3343
                     /locus_tag="VFD89_06425"
                     /inference="COORDINATES: protein motif:HMM:NF023435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZJ57861.1"
                     /translation="MKKLWITRTLLFLLVIAMVIPLFACSKPAVTPEKPEDTAKVPEE
                     KPDDSEKEDEEPEPKDELEFVTLDWYLGLSPMPDNEMVNDAVNDYLKEKVNANVNIFY
                     WGASDWETKMTTMVSAGQDVGIIGFGSQSKLDYVVQASRGSFYPLNDLLNEYGAGTKA
                     LFSDEIWDCMTINDNIYGIPSLKDNCYIISIVYNADMAEALEIDMDALEYKNWRNIEP
                     FLEDVLAKRTEKFPEYDEYPLCGGAFLEMPYNFAIETFLNDSFLLACNINPFNDIAGY
                     DDNTVFNLYATDEYREFAKSRQRMVDKNIFAYDYTDKSEWNYTGGMFAWVGWGYTYME
                     EHLYGDAFKTKMVVSDNIWTDTNNYFSAGTAISANCADPERAMMILNLVNTDPEFATM
                     MRFGVEDKHYVINDEGKMQFEGSERNGGDRADYGYYYWYAAPVGNLTIVNAPESLTGP
                     DGIMLTEMVNYNNNAVIPAHMGFVFDTEPITNELASCTSVVMEYRDVLRNGQLGSVAE
                     VDAAVDEFNQKLKDNGVEKLLTEAQSQIDAWNAAR"
     gene            3441..>5461
                     /locus_tag="VFD89_06430"
     CDS             3441..>5461
                     /locus_tag="VFD89_06430"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HZJ57862.1"
                     /translation="MSTLKFTHYEMPSASLGKLNPLPDLKKTVDAHASIPIDESTVTR
                     EESKYMGWGRVNGILPYLLQDGYDRKKKPRKWNAAILENDSLRAVFLPEIGGRLWSLV
                     DKTTGKELLHKNPVFQPCNLGLRNAWVSGGVEWNIGIIGHTPFTVDSMFAEELALDDG
                     TPVLRMFQYERIRRLVYRIEAMLPKDSKYLYVRVRIDNPLDEDTAVYWWSNIAVDERE
                     DVRVLVPSDKSYRWGYGGNLSKVSIPYMKVKIPKAGHDGEQPQQECWDISRTTQLPQA
                     MDFFFDIPADNRPWISAVGDDGYGFVQTSTDELRGRKLFVWGMGAGGRNWQTFLSQPG
                     HAYLELQAGLARTQLEHLPMKANETISWMEAYGPISAEADIVHGSDWDSAAKAIEESL
                     EKACPQAMVDDMHQRAKSELDGKAGDLRSVGEGWALVDRELLGERFRDVELEFPRLNL
                     GEREEPWLKLVREGSLPCPEVLEEPGGYQIGSEWESLLEKSIKGGGSRHWYGYYQHGV
                     MLAHGGRMEEAEVAFDKSLECEISPWALRSKALLRDLAGDQESAARLLLQAVVMLPEP
                     NIAKETLQILSKAGLHSEIIELYHKLPEEVKGLGRLKVILIEALLDTGDIDGVEDMLK
                     GDIELTDVREGEVKLTDLWFRMTAMKRAREFGVEADDDLLKQVQRECTPP"
BASE COUNT         1503 a         1205 c         1405 g         1348 t
ORIGIN      
        1 tcgctgggga acctttgcca aagtaatgca aaccacctat atgatgccgt attttttgtc
       61 ctatgcggtg gttgccatcg tagcaaacgc ctttttacat agggacaatg gacttatcaa
      121 ttctttaata aggatgtcgg ggggagtcgg gaagacaaat tggtatcacc agttgcggct
      181 atggccccca ttattggttt ttgtcaatgc atggaagggc gtgggctatc aaactgtgtt
      241 gtatttagcc gttatttcgg gaatatcagt agagtactac gaagctgcag tactagatgg
      301 cgcatctaag atgcaacaag cccgctacat cacaataccc cacctcaggt atatagtggg
      361 gatatcctta atcctggcca tgtcctctat cttccgcggg gatttcggat tgttctacaa
      421 tgtgcctatg aatcagggga tgatctatcc ggtaacggat gttatagata cctacatata
      481 tagggctctt atatatctaa acaatgtggg catgtctaca gcagccggtc tgtatcaatc
      541 cgtggtggga ttcatattag tgatcatagt caatcatata gtaaatagaa ttgatcccga
      601 gagtgctatg ttctaaggga cttatatggg attaggaaag gagatctaaa catggcaaaa
      661 gaggaaaagc atataaataa gctcaactca atttcagagg gttggaatat ggtgttcgtc
      721 attattctgt tgattatagc cctgttagtt ataataccca tggtgttaat aatcgtaata
      781 tccttttcaa gcgagatcag catagccgac aagggattct cattcatacc tacccaatgg
      841 tccataagcg gctataagta cttactcaaa acgggggatc agcttttaga ttcctatatc
      901 attacaatca tctattccgt tacggggaca ttcatgagtc tggccgttat gaccatgtat
      961 gcatatgtca tatcccaaag gaatttcaag ttttcccggc tgtttacttg gctcctgttc
     1021 ttcaccatgc tctttggagg aggactggta cccagttata ttctaaatgt acgctaccta
     1081 catataaaca acaccatatg gatattctta cttccttccc tggtaaatgc atattatact
     1141 atcatactac gtacctttat cagaaccact atcccggata gcctctttga tgccgcgcgt
     1201 attgatggag ccgggcattt tcgtatcttt ataagcattg tgcttcccct ctttaagacg
     1261 ggtatcgcca cggtgggtct tttcggcctg gtgtcccgtt ggaacgactg gtttataggc
     1321 atgctctaca tacaaaaccc aaaattgatt cctcttcaga ccctgcttac caaactacag
     1381 gatagtatca aattcctcaa agaaaatgcg gctattgcag ggacgccgga cggtatagca
     1441 ctgcttcggg cactacctca ggagagccta cgtatggcct gcaccgtagc ggttattctt
     1501 cccatccttt tcgcgtaccc cttctttcag cgctactttg tacagggcct gaccataggg
     1561 agtatcaaag gttgatgaat atgggctccc catatcataa ggggggatac atagacccgg
     1621 tcgcggccta aagagtcagg tctcggcctg gacaaattta aaaatccgcc atattaaggc
     1681 gggacaatat attattaaag gaggatcttt catgaagaag ctgtggatca cccggacact
     1741 tctattccta ttggttatag ctatggttat acccctcttt gcttgttcga aaccggctgt
     1801 aaccccggaa aaaccagagg acactgccaa agtccccgag gaaaaacccg acgatagcga
     1861 aaaagaagat gaggaacccg agcccaagga cgaattggaa tttgtaaccc tggactggta
     1921 tctgggccta agccctatgc cggataacga gatggtaaat gatgccgtca atgactacct
     1981 taaagaaaag gtaaatgcca atgtcaatat cttctattgg ggcgcaagcg attgggaaac
     2041 aaagatgacc accatggtaa gtgcgggaca agacgtgggc ataatcggat ttggcagcca
     2101 atccaaactt gactatgtgg tacaagcaag ccggggttct ttctatccct taaatgacct
     2161 actaaatgaa tacggcgcag gtaccaaagc cctattctcc gatgaaatct gggattgtat
     2221 gaccataaac gataatatct atggtatacc gtccctcaag gataactgct acatcatatc
     2281 catcgtatat aatgcggata tggcagaggc tcttgaaatc gacatggatg cactggaata
     2341 caagaactgg cggaatatag aaccatttct agaggatgtt cttgcaaagc gtacagagaa
     2401 attccccgag tatgacgaat atcccctatg cggcggagca ttccttgaga tgccatataa
     2461 ctttgccatt gaaaccttcc taaacgattc cttcctgctg gcatgcaata tcaatccctt
     2521 caacgatata gcgggttatg atgacaacac cgtattcaac ctatacgcaa cggatgagta
     2581 ccgtgaattt gccaagagca ggcagcggat ggtggataag aacatatttg cctatgatta
     2641 tacggataag agcgaatgga actataccgg cgggatgttc gcctgggttg gttggggcta
     2701 tacctatatg gaagagcacc tctacggtga cgcattcaag accaagatgg tagtttccga
     2761 taatatatgg acggatacca acaactattt cagcgcaggg accgctattt cagccaactg
     2821 tgctgatccg gaacgcgcta tgatgatcct aaatctggtt aataccgatc ccgaattcgc
     2881 aaccatgatg cgctttgggg ttgaagataa acattacgtc atcaacgatg aaggcaagat
     2941 gcaattcgag ggcagcgaac gaaacggcgg agacagggca gattacggtt actactattg
     3001 gtatgccgca cctgtcggga acttgaccat agtcaacgcc cccgaatccc taaccggccc
     3061 agacggaatc atgttgacag agatggtaaa ttacaacaac aatgcagtta ttccggcaca
     3121 tatgggcttt gtatttgata ccgaaccgat taccaatgag ctggcgtcct gtaccagtgt
     3181 cgttatggag tatagggacg tacttcgaaa tggacagctg gggtctgtgg cagaagtgga
     3241 tgcagctgtt gacgaattta accaaaagct taaggataac ggggttgaga agctgctgac
     3301 cgaggcccaa tcccaaattg acgcatggaa tgcagccaga taaactcagc tgagagctaa
     3361 tctattgtag taacggtggc tccccataac ttataagggg agccacaaca tgttcaaaaa
     3421 tagttaagga gtgatagata ttgtctacct tgaagtttac ccactatgag atgccgtcag
     3481 cttccctagg taaactcaac ccattgccgg atctcaagaa gacagtcgat gcccatgcat
     3541 ctattcccat agacgaatct actgttactc gcgaagaatc caaatatatg ggctggggta
     3601 gggtaaatgg catactgcct tacctattac aggacggtta tgaccgtaag aaaaaacctc
     3661 gcaagtggaa cgcggctata ctggagaacg actctttacg tgcagtattc ttaccggaaa
     3721 ttggcggacg cctttggtct cttgtagata agactacggg gaaggagctg ttacacaaaa
     3781 atcccgtatt tcaaccctgc aatctggggc tgcgcaacgc ctgggtttcg gggggcgtgg
     3841 aatggaatat aggaattatc ggacacaccc catttacggt ggactctatg tttgccgagg
     3901 aattggccct tgatgacggt acgccggttc tgcgaatgtt ccaatatgag cggatacgaa
     3961 ggctggtcta ccggatagag gcaatgctac ccaaggattc taagtatctg tacgtacggg
     4021 tccgcataga taatccgttg gatgaggata cggccgtcta ttggtggagc aacattgcgg
     4081 tggatgaaag ggaagatgta agggttttgg ttccgtcgga caaatcctat cgttggggat
     4141 atggaggaaa cctcagcaag gtctccatcc catatatgaa ggtgaagatt cctaaagccg
     4201 ggcatgacgg cgagcaaccc caacaggagt gttgggatat aagccgcacc acccagctgc
     4261 cccaagctat ggattttttc tttgatatcc cggcagacaa caggccctgg atatcggctg
     4321 taggcgatga cggatatggg tttgtacaaa cttctaccga cgaactccgt ggaagaaaat
     4381 tgtttgtctg ggggatgggt gccggcggca gaaactggca gacattccta tcccaaccgg
     4441 gtcacgcata cctggaattg caggccggtc tggcccgcac ccagttggag cacctgccta
     4501 tgaaggccaa cgagaccatc agttggatgg aggcttatgg cccgatttcg gccgaggccg
     4561 atatcgtcca tggaagtgat tgggattccg ccgccaaagc tatagaggag agccttgaaa
     4621 aggcctgtcc ccaggctatg gtggatgaca tgcaccaaag ggctaaaagt gagctggatg
     4681 gtaaagcagg ggatctaagg tctgtaggcg agggttgggc cctcgtggat agggaacttt
     4741 tgggggagag gtttcgggat gtggaactgg agtttcctag gttgaacctc ggagagcgag
     4801 aagaaccatg gctcaagttg gtccgtgaag gcagtcttcc ctgccccgaa gtgcttgaag
     4861 agcccggcgg ctatcagatc gggtccgaat gggaatccct attggaaaaa tccatcaaag
     4921 ggggaggaag caggcattgg tacggctatt accaacacgg ggtaatgttg gcccatgggg
     4981 gaaggatgga ggaggcggaa gttgcctttg acaaatccct ggaatgcgaa atcagcccct
     5041 gggctcttag aagcaaagcc cttttaaggg atttggccgg ggaccaagaa tccgctgcca
     5101 gattgttgct ccaggccgta gtcatgctcc cggagcccaa tatagcaaag gagaccctcc
     5161 agatactgag taaggccggg ctgcatagcg agatcataga gctttaccat aagctaccgg
     5221 aagaagtcaa gggactggga aggctcaagg tcatattgat cgaggccctt ctcgatacgg
     5281 gggatataga cggggtagag gatatgctaa agggtgatat agagcttacg gatgtaaggg
     5341 aaggggaggt taagctcact gacctgtggt tccgtatgac ggctatgaag cgggcccggg
     5401 agttcggagt agaagcagac gatgatcttc tgaaacaggt ccaaagggaa tgtacaccgc
     5461 c
//
LOCUS       DASRNX010000161         9312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_191836,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000161 DASRNX010000000
VERSION     DASRNX010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191836"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFD89_06435"
     CDS             <1..627
                     /locus_tag="VFD89_06435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZJ57863.1"
                     /translation="LYLIEQVLVADYTTKTIAKVVLFVGIPSVYSALMKNSSIRGIIR
                     LIKVEINTLKYGLTLGLISFASIFAGYFVLGKYIDFNGIVQELGSMSGITASNFIFTG
                     LYITFGNSFLEEFFFRGFIFLNLFYRGSKKFAYIYSSLLFGIYHIAIFKTWSSPALTA
                     LALLGLVSVGMVFNRLDTKTGNFMNSWLVHILADSAIIIIGMKMFNII"
     gene            726..2141
                     /locus_tag="VFD89_06440"
     CDS             726..2141
                     /locus_tag="VFD89_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZJ57864.1"
                     /translation="MNNNSLSKNNTRLITTKKKLLFITAGLFILCIISYTSISLYYNT
                     HFYINTVINGVDTSNMTVEQAEEAIQEQFKNYSLSLNGRNDLTEQIQGRDFNIYAKFD
                     GSLDELLDTQNSFMWVGALFKTPKMEIKTRPEYDADLLREVVSNLAYFQEENMIKPVN
                     AYISEYDKNKGYEIIPEVFGAWPDFDILFGAVKAAIANLEPTISLEDIGCYKKPEIIS
                     ESHNLVRVTNELNKIAGAEIVYEFGRDTEILDGTLISEWLSVSEDDNVILDETGVKEY
                     VDYIGKTYNSFGRTRTFKTSYGTTIKVKGGDYGWWLNRPLEVSELIELLLAGEKAKKE
                     PAYFQTAQQYGPDDVGDTYVEVNLKAQHLFFYKNGKLIVETDFVSGNLAKGYGTPPGT
                     YPVQYKERNATLKGEDYTTPVDYWMPFNRGIGFHDATWRNKFGGDIYKTKGSHGCINM
                     PPKAAKKMFEHIKRGVAVIVY"
     gene            complement(2223..4415)
                     /locus_tag="VFD89_06445"
     CDS             complement(2223..4415)
                     /locus_tag="VFD89_06445"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZJ57865.1"
                     /translation="MDRIISRFVPMMLSLSVSGTILALIIISLKVVVKQAVSKTFMYY
                     VWILVLLRLVIPFGLWDGLFRTTSSGFYALTQVNNDFHGNSQYPGAYPHGEEISTDME
                     NIRPSYSPASQSISNEPMDITPNKIPWVNSSRAFIFFIWLMGALITFSYSFISYLLFT
                     KKTIQTGNVPPKRINDIFRSLHNFRNLGLICSKYAKTPMQIGIIKPYIVIPQGRYEES
                     EIELKHILRHEIIHYRRGDLVYKWLVHLVASLHWFNPFMIIIRKSVEDACELACDEEV
                     IRNMLARQRMEYGEVLISQSHESGISAGRLAASLYYPKNNLKERILGIKTYRPRSLAT
                     NILALILAIALTGCAFSLGPEVPDGDTNPSAKDFILQEEGSKTDDNGYPMDDIPNLTT
                     YLSGSDNSLRLKPINIESKMPMFELPDKSPLLYPDSKGQIYKVEPVDVRANIQTAEHT
                     AHELWDNVTQMYSESSLNLNPNILAFVSDTAGAHYGSYRESIRIDGRDPLNMEYLGYD
                     NAFLDNPALSPLPGQDSLKRSDNEFISIAKNFASRIPETADYIVDVPRVHKVLMEYYR
                     SNLEGDVQIPDVTAVCWDHWVDGLRVYNDGLYISFLGNNDRPLSLSLRKHLLSKIDGE
                     NPDFLLRADEALHCINYVRSNRELWSTNPPGMAIFSNRQEIVSVNPILSNVFSNKGDV
                     FVPTWEFIIFPFPHPDSDCFSMDILVNALTGEVYIGANGRLYSPPLEM"
     gene            complement(4419..4784)
                     /locus_tag="VFD89_06450"
     CDS             complement(4419..4784)
                     /locus_tag="VFD89_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010277591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HZJ57866.1"
                     /translation="MIDKITESEFEIMKALWDYENPLTFSDIRKYVQRNNGWSPSTIK
                     TLLYRLCEKGAVSQKKEDVYYYRPLISQEEYSDHTTQSLIDKLYNGKAANLIATLVKN
                     DTLAKADIDELRKLLHGED"
     gene            5317..6726
                     /locus_tag="VFD89_06455"
     CDS             5317..6726
                     /locus_tag="VFD89_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57867.1"
                     /translation="MIKFRSHTKARLFLVLFTISILLAACGGEGVDMGDKYVHGQDSQ
                     FFYSPVFEEGPYLATTEDGYYFVINAFVYFIDKETLKAVPLCNKPNCKHDSDECNAAF
                     HTYDNFLSFYKGSLYVKGEASDKEERALKNYIYQVSLDGSERKTVLEYKDGFQFGEPK
                     TIHRGYIYFENITPFIWESINVGASISRYDLKDPSEREILFESDLEDANVRLGSIYGN
                     YLYFYELGYRGEENISRSMIYNLETGEIKEMGNLGDDKIHPHFSTIFGGKLMYLGALY
                     ITDYDDYDVVEELGGKDAYLKAKNLTTIPLYEADLWGENPKKVMDINIINSNSDIIAD
                     SDYVYEYSYNYYRFLERRWLQEEGSEADPNECLKNRYLKVMDKDFNEVAMVDIGSLNL
                     PGNYLQIYLTQGEHIFLKAFDRGNVDDTDYADGLNDGDEVLYVIDKKDFGKEKVEVRE
                     ILRRQIQDMGWSIDYNELP"
     gene            6856..8988
                     /locus_tag="VFD89_06460"
     CDS             6856..8988
                     /locus_tag="VFD89_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57868.1"
                     /translation="MDDTAKIYEKYGIYEKLSAHINYLDGYGEYIESIKDKADEMLTF
                     SIFFKKGSFSYRNILKTPGDYQHLRGIPIKLGIEEGIIAATNFRIADILVIIFTFLVC
                     ALLFWHEKENGIFNLIKSCKNGRTGAIAAKLMATGLVCILSAVILYGSVFCISGWIFG
                     FGDTSRFIQSMPYFEKSNLLLTTGQYIMLFLMGKVSVVLLISLVFALLFMMFDNSTKV
                     YLSIIAILAAGYTTYTYIMPLSPFNIFKYINIFAFLDMGDLLGRYNNISFFGLPIGRV
                     TISGIVIFASIMSLSITNIMLFAVQRKATTRDSFGPLGERLKGFLYRRFKWLGSTSLI
                     WHESYKLMFSGGAILIILSLIYIGHSDLLKKAPESDFYEMVYDGYMEKLEGAITPEKE
                     TFILNEREYFETLSDRQTSLGEMYESNEITKAQYDIAMADIASSQVRERVFNDIEEQY
                     LYLLDLEGSGRTASFINRINSDYIFHYKQRDIKNALILSAMIIIAISTVFCREYKNDM
                     INILLPTKNGRSDLFIRKVIVSSVLAILLTLLVYLPEYINMLKFYNLGDYRASVQSIQ
                     RFKNLGSINIGPKNLRYVSPGPNEIGLGSPGFNISIGGFIAAKWLVTILGAVSMSLFV
                     IFISIISKSQAITVFIAALSIIAPILMEALGMGILRFFSYNNTFLINNTLEKEKGTGI
                     AIVYLLGMVLVTVCMTRLSYKKYTRINR"
     gene            9150..>9312
                     /locus_tag="VFD89_06465"
     CDS             9150..>9312
                     /locus_tag="VFD89_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZJ57869.1"
                     /translation="MILSIDSVSKTYQKGRKKALDSFSAELTAGVYGLLGPNGAGKST
                     LMNIITDNIK"
BASE COUNT         2807 a         1716 c         2076 g         2713 t
ORIGIN      
        1 ctgtatttga ttgagcaagt tttagtggcg gattatacaa ccaagaccat cgcaaaagtg
       61 gttttatttg taggcatacc atccgtatat tcggcactta tgaaaaattc cagtattagg
      121 ggaattataa ggcttataaa agttgaaata aatacactaa aatatggctt aaccctcggt
      181 ctgatttcct ttgcatctat atttgcaggc tattttgtac tgggaaaata tattgatttt
      241 aacggtattg tacaggaatt agggtctatg tccgggataa cggcatcaaa ttttattttc
      301 acaggactat atataacctt tggtaattca ttcctggaag aattcttctt caggggattt
      361 atatttctga atctctttta taggggatct aaaaagtttg cttatattta ttcttcatta
      421 ttgtttggga tataccatat agcgattttt aaaacatggt ctagtcctgc cctaacggct
      481 ttagcccttt taggcctggt cagcgtgggc atggttttta accggcttga taccaaaacc
      541 gggaacttta tgaattcatg gctggtacat attttagcgg atagcgccat aataataatt
      601 ggcatgaaaa tgtttaatat aatctgatta atggtatagt tatgataatc ttgggcaatt
      661 tgggggaatc ctccttagaa taatagtata aagggcaata cgccctgtga aaagggtggt
      721 tcgatatgaa caacaattca ttaagcaaaa ataataccag gttaataaca acgaaaaaga
      781 agctgctatt tataacggcc ggacttttca ttctgtgcat aatatcatat acatccataa
      841 gcctttatta taatacccat ttttatataa ataccgtcat caatggagta gacacctcta
      901 atatgacagt ggaacaggca gaagaggcga tacaggaaca atttaaaaac tattccctta
      961 gccttaatgg acgtaacgat ttaaccgaac aaatccaggg cagggatttt aatatatacg
     1021 caaaatttga tgggagcttg gatgaattac tggatacaca aaatagtttt atgtgggtgg
     1081 gtgcattgtt taaaaccccg aagatggaga tcaagaccag accggaatat gatgcggatc
     1141 tattgaggga agtagtatcc aatcttgcct actttcaaga agaaaatatg ataaaacccg
     1201 tcaacgccta tatatctgag tacgataaga ataagggtta cgagataatt cctgaggtct
     1261 tcggagcgtg gccagatttt gatatattgt ttggggcagt gaaggcggcc atagccaatc
     1321 tggagcctac gatatcattg gaggatatcg gttgttataa aaagcctgaa atcatttccg
     1381 aatcccataa tctggtcagg gtgacaaacg aattaaataa aattgcagga gcggagatag
     1441 tctacgagtt tggcagggat acggaaattt tagacggaac cttaataagc gagtggctct
     1501 ccgtttcaga agacgacaat gtgattttgg atgaaaccgg tgtgaaagaa tatgtggact
     1561 atatagggaa aacatataat tccttcggaa gaacacggac ctttaagacc tcctatggca
     1621 ctaccataaa ggtcaaaggc ggagattacg gctggtggct gaatagaccc ctggaggtct
     1681 ctgaacttat agaattactc cttgccggag aaaaggcaaa gaaggagccg gcttattttc
     1741 agaccgcaca gcaatacggt ccggatgatg taggggatac ctacgtggag gttaacttaa
     1801 aagcccaaca cctatttttc tataaaaacg gcaaactgat cgtggagacg gactttgtgt
     1861 caggaaatct tgccaagggg tatggcaccc cgccgggtac ctatccggtt cagtacaagg
     1921 aaagaaacgc taccttaaag ggggaggact acaccacccc ggtggattat tggatgccct
     1981 tcaaccgggg catcggcttt catgatgcta cctggcgaaa caagttcggg ggagatatat
     2041 ataagaccaa gggttcccat ggatgtatca atatgccccc aaaggcagca aaaaaaatgt
     2101 ttgaacatat aaagcggggc gtagccgtca tagtatacta ggcaccaaag atgccaaggg
     2161 gatgtttcgg atgtcgtaaa cgttgacctc aattcatatg ttgcggaaaa tacatttttt
     2221 aattacatct caagtggcgg cgaatacagc ctaccatttg cgcctatgta tacctcacct
     2281 gtgagtgcgt taacaaggat atccatggaa aaacaatcgg aatcagggtg ggggaaggga
     2341 aatatgataa attcccatgt gggaacgaat acatcccctt tgtttgaaaa tacatttgaa
     2401 agaattggat ttacagatac tatttcctgc ctatttgaga atatggccat gccaggtggg
     2461 ttagtactcc ataattcgcg attgcttcgg acataattta tacagtggag ggcttcatcg
     2521 gccctaagga gaaaatccgg attttccccg tctatcttag acagcaaatg ttttcttaag
     2581 gacaaggaca gaggtctgtc attgtttccc aaaaagctta tgtacaaccc gtcgttatat
     2641 actcgtaatc catcaaccca atggtcccag cagaccgccg ttacgtcagg gatttggaca
     2701 tcaccttcaa gattggatct ataatactcc attaggactt tatgtaccct tggcacatcc
     2761 actatatagt cggctgtttc tggaatacgg gatgcaaaat tcttggcaat ggaaatgaat
     2821 tcattatcgc ttcttttaag ggaatcctgc ccggggaggg gagacaaagc ggggttgtcc
     2881 aagaacgcat tatcgtatcc taagtattcc atattcaatg gatcgcggcc gtctattcta
     2941 atactttctc tatatgagcc ataatgggcc cctgccgtgt ccgatacaaa ggctagaata
     3001 ttgggattaa ggttcaggct ggattcggaa tacatttggg ttacgttgtc ccatagctca
     3061 tgggcagtat gctcagctgt ttggatgttg gccctaacat ctaccggctc cactttatat
     3121 atttgaccct tgctgtccgg atagagaagg ggagatttat cggggagttc aaacatgggc
     3181 atcttgctct caatgtttat aggcttaagc ctgaggctgt tgtcgctacc tgaaaggtag
     3241 gtggttaaat taggaatatc gtccatgggg tatccgttat catccgtttt cgaaccctcc
     3301 tcctgcagga taaaatcctt ggctgatgga ttggtatcgc catctggcac ctccggtccc
     3361 agactaaagg cacagccggt cagggctatc gccaggatta aggcaaggat attagtggca
     3421 agggaacggg gacgataggt ctttatgccc aggatcctct ctttcaggtt attctttgga
     3481 taataaaggg aggctgccaa ccttcccgcg gatatcccgg attcatggga ttggcttata
     3541 agaacttccc catactccat acgttgccgt gctaacatat tccttatgac ttcctcatcg
     3601 caggccaatt cgcaggcatc ttctacagac tttcgaatga taatcataaa aggattaaac
     3661 caatggaggg acgcgacaag atggacaagc catttataga caagatcccc tctcctatag
     3721 tgtataatct catgacgcag gatatgtttt aattcaattt cactttcttc gtatctcccc
     3781 tgagggatga cgatataggg cttgatgatc ccaatttgca tgggcgtttt ggcatatttg
     3841 ctgcaaataa gccccaggtt tctaaaatta tgtagggagc gaaatatatc atttatccgt
     3901 ttagggggaa cattgcctgt ttgtatagtc tttttagtaa atagaagata ggatataaaa
     3961 gagtaggaaa aggtgatcag agcccccatg agccatataa aaaagatgaa agccctgctg
     4021 ctatttaccc atgggatttt atttggtgtt atgtccatgg gctcgttact tatgctctgg
     4081 gaagccggag aataggatgg ccggatattt tccatatcgg tggatatttc ctcgccatgg
     4141 ggataggcgc cgggatattg gctattccca tgaaaatcgt tatttacttg ggttagggca
     4201 taaaatccgg aggaggttgt cctaaagagc ccatcccata aaccaaaggg tatcacaagg
     4261 cgcaagagca ccagtatcca tacatagtac atgaatgttt tagagactgc ctgcttaact
     4321 acgactttta gcgatattat tatcagagcc aaaatggttc cggaaaccga taatgagagc
     4381 atcataggca cgaaacgaga tataattcta tccatgattt aatcctcccc gtggagcagt
     4441 tttcggagtt cgtctatgtc agccttggcc agggtatcat tttttactag agtggcgata
     4501 aggtttgcag cctttccatt gtatagctta tcaattaagg actgggtagt atggtcgcta
     4561 tattcttcct ggcttattag aggtctatag taatacacat cctctttttt ttgtgataca
     4621 gcgccttttt cacagagcct atagagtagg gtcttgatgg tagatgggct ccacccattg
     4681 ttcctttgaa catatttgcg tatatctgaa aatgtcagcg ggttttcata atcccacagg
     4741 gctttcatta tttcaaattc agattcggta atcttgtcga tcaaatcact ttcatccttt
     4801 ctgtgtatag cttattgtat ataaaaagct ttgaaaaacg atgcccaatt atcaaggtaa
     4861 taatcccaat cctgtgggtt gtctgcaccc tggacggtta gtcgtctatg ataatatgta
     4921 gcccgataca gaatttagat atataacgtg acaaatgtag catgtactta tataatattt
     4981 tatatattgc cctatgtcaa ccctaagatc tgagagtttt ttctgttgaa tggatttatt
     5041 ttgcgtacca taaaatgaat gatttaatcc cataggaatt tgggaatttg ggaatttggg
     5101 aatttgagtt ttccgtatta taccttaaat tagcggataa tacgctttgg gaatcccacc
     5161 aatcaataga tataaaaacc tcctattggt gcatcaatat ttaataaatg ggattttaaa
     5221 acaaattaat gttgacaagc cagaagataa tatggtagga tatccatagg tgacaaatgt
     5281 cacccgagat agaactttat catggaggga ttgggtatga ttaaattcag aagccatact
     5341 aaggctaggt tatttcttgt gctttttacg atatccattt tgctggctgc ctgcggcggg
     5401 gaaggcgttg atatggggga taagtatgta cacggccagg attcacagtt tttttatagc
     5461 cccgtttttg aagaaggccc atatctggcc actaccgaag acggttacta ttttgtgatt
     5521 aacgcctttg tctattttat tgataaggag accttaaaag ccgtacccct atgcaataaa
     5581 cccaattgta agcatgacag cgatgaatgt aatgccgcct ttcataccta tgataacttt
     5641 ctcagcttct ataagggaag cctctatgtt aaaggggaag cctcggataa ggaggaaagg
     5701 gcgctaaaga attacatata ccaggtttcc ctcgatggca gcgaaagaaa gacagtgctg
     5761 gagtataaag acgggtttca gttcggggaa ccaaaaacca ttcatagagg atacatatat
     5821 ttcgaaaata taaccccctt tatatgggag agcattaatg taggcgcctc aatatcaaga
     5881 tacgacttaa aggatcccag tgaaagggag atcctattcg aatcggattt ggaggatgcc
     5941 aatgtcaggc tcggcagtat atacggaaat tatctgtact tttatgaatt gggttacagg
     6001 ggcgaggaaa acatttcaag atccatgata tataacctcg agacagggga gataaaagaa
     6061 atgggtaatc ttggggatga caagattcac ccacattttt caacgatctt tggcggaaag
     6121 ctaatgtatt taggggctct ttatatcact gactatgatg attatgatgt ggtagaggag
     6181 cttgggggca aggacgcata tcttaaggcc aaaaacttaa ctactatacc cttatacgaa
     6241 gcggatttat ggggagaaaa cccaaagaag gtcatggata taaatatcat taattccaat
     6301 tctgatataa tagccgattc tgattacgtc tatgagtata gctataatta ttaccgcttt
     6361 ctcgaaagac gctggttgca ggaggaaggc agcgaagcag atccaaatga gtgcctaaaa
     6421 aatcgatact taaaggtcat ggataaggat ttcaatgagg ttgccatggt ggatataggg
     6481 tctttaaacc taccggggaa ttatcttcag atatatctaa cccaaggtga acatatcttc
     6541 ttaaaggcat ttgacagagg caatgtggac gatacggatt atgccgatgg tttgaatgat
     6601 ggtgatgagg tcctctatgt tatagataaa aaggattttg ggaaagaaaa ggtcgaagtt
     6661 agagaaatct taagaagaca aattcaggac atgggatggt ccattgatta taatgaactg
     6721 ccctagtccg gcatttgaat aaaacccaaa atctaagggt gaaacataag gacaagggtt
     6781 tgcatagcta tagataaaca acttcaattc tataataaaa acccggaatt ggttgaggag
     6841 tatattacta aatacatgga tgatacggcg aaaatctacg aaaagtatgg catctatgaa
     6901 aagctaagcg cccatataaa ctatttagac ggatatgggg aatatataga gagtataaag
     6961 gataaagccg acgagatgct gaccttttcg atatttttta aaaaaggcag cttctcctat
     7021 agaaacatat tgaaaacccc gggagattat cagcacctta ggggcattcc gataaaacta
     7081 ggaatcgagg aaggtataat agccgcgacc aattttagaa ttgccgatat cttggtgata
     7141 attttcacct ttctagtctg cgctttactt ttttggcatg agaaagaaaa cggaattttt
     7201 aaccttataa agtcctgcaa gaatggcagg acaggggcta tagccgcaaa gcttatggcg
     7261 acgggactgg tatgcatcct atcggccgtt attttgtatg gatctgtatt ctgtatctcc
     7321 gggtggatat ttggctttgg ggatacatca agattcatac agtccatgcc ttattttgaa
     7381 aaatccaatc tcttgttaac caccgggcag tacataatgt tgtttctcat gggcaaggtt
     7441 tccgttgtcc ttcttatatc ccttgttttc gcacttctat ttatgatgtt tgataacagt
     7501 acgaaggtct acctttctat catagctata ctggcagcag gatatacgac ctacacatac
     7561 attatgcccc tctcaccctt taacatattc aaatatataa atatatttgc ctttttggat
     7621 atgggcgatc tacttggcag gtataacaat atatcatttt tcggacttcc tataggtagg
     7681 gtaacgattt ccgggatagt aatttttgcc tcaataatgt ctttaagtat aacgaacatt
     7741 atgttgttcg ctgtacagag gaaggctacg acaagggatt cgtttggtcc tttgggtgaa
     7801 aggctaaagg gatttttata tcgccgtttc aaatggcttg gaagcaccag tctaatctgg
     7861 cacgaatcct ataagttaat gttttcagga ggggccattc ttataatatt atcccttatc
     7921 tatatagggc atagtgacct tctaaagaaa gcaccggaat cggatttcta tgagatggtc
     7981 tatgacggtt atatggaaaa gttggaaggc gctataaccc ctgaaaagga gacgtttatc
     8041 ctaaatgaga gggaatactt tgagacctta agcgacagac agacaagtct aggcgaaatg
     8101 tatgagagca acgaaataac aaaagcccaa tacgatatag ctatggcgga tattgcctca
     8161 agtcaggtca gggaaagggt gttcaacgat attgaagaac aatatctata tctcctggat
     8221 cttgagggaa gcggaaggac tgccagtttt ataaacagga ttaattccga ttatatattc
     8281 cattataaac aaagggatat aaaaaatgcc ttgatcctct cggctatgat tataattgct
     8341 attagtacgg ttttttgtag ggaatacaag aatgatatga tcaatatcct tcttcccacg
     8401 aaaaatgggc ggagtgacct atttatcaga aaggtgatag tgtcatcagt tcttgctata
     8461 cttttaaccc tgttggtata cttgccggag tatataaata tgcttaagtt ctataatttg
     8521 ggtgattata gggcatccgt tcaaagcatt caaagattta aaaacctagg gtctataaat
     8581 atagggccta aaaacctaag gtatgtcagt cccggcccta atgaaatagg gcttggaagc
     8641 ccgggcttta atatttcaat aggcgggttc attgccgcga aatggctggt gactattctc
     8701 ggtgcggtat ccatgtcctt atttgtaata tttatatcca tcatatctaa aagccaggcc
     8761 ataaccgttt tcattgcggc gcttagcatt atcgccccta ttctaatgga ggccctgggc
     8821 atgggaatcc ttagattctt ttcctataat aatacttttc ttatcaacaa taccttagag
     8881 aaagaaaagg ggacgggtat tgccatagtt taccttctgg gtatggtact ggttacggtt
     8941 tgtatgaccc gcttatcata taaaaagtac accaggatta accgatgacc gttaaaacct
     9001 gcccaaatcc cttattttta tgaaagagga tatggttttt gtgatatatg tatccgaagt
     9061 ggtactccac ttgacccaaa ttccggtgca ggatgtttat cataaaagac catagaagga
     9121 atcccagttt ttttattgga ggtgtagagg tgatcttatc catagacagc gtatcaaaaa
     9181 cctatcaaaa aggtagaaag aaggccctgg acagctttag cgcagagctg acagccgggg
     9241 tatacggatt gttaggccct aacggggcgg gaaaatccac tctgatgaac attataacgg
     9301 ataatataaa ag
//
LOCUS       DASRNX010000162        17190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_404733,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000162 DASRNX010000000
VERSION     DASRNX010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17190
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404733"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..2002
                     /locus_tag="VFD89_06470"
     CDS             326..2002
                     /locus_tag="VFD89_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HZJ57870.1"
                     /translation="MSNNKENTKVEVNIGSNFIHDIIDRHLEDGVYKNVHTRFPPEPN
                     GYLHIGHAKSICLNFGTADKYNGLCNLRFDDTNPLKEEVEYIDSIIEDVRWLGFDWED
                     RLYYAADYFDKMYEYALLLIKKGKAYVDDLTAEEIREYRGTLTQPGKNSPYRDRGVEE
                     NLDLFERMKAGEFEDGSRVLRAKIDMASPNMNMRDPVIYRIAHVPHHRSGDKWCIYPM
                     YDYAHPISDALENITHSICTLEFEDHRPLYEWVLDEIDLFPNPPIQIEFARLELTNTV
                     MSKRKLRVLVEEGKVAEWDDPRMPTLSGLRRRGYTPSSIRDFSERVGIAKNNSVVDVA
                     LLEHCLREELNRDAQRVMGVLDPIKLVITNYPEDQVEYMEVENNQEDPSTGTRMVPFA
                     RELYIEREDFMEDPPKKFFRLTPGKEVRLKNAYIIRCEGVIKDQETGEILEVHCTYDP
                     DTKSGSGSANRRVRGTLHWVSVVHGIPVMIRLYDYLFTEDEDGGMQYNPDSIKELKGY
                     VEPSLEGTEGGSRFQFFRQGYFCTDIKDSTPDNLVFNQIVPLKSSYRPNR"
     gene            2069..3532
                     /gene="gltX"
                     /locus_tag="VFD89_06475"
     CDS             2069..3532
                     /gene="gltX"
                     /locus_tag="VFD89_06475"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZJ57871.1"
                     /translation="MRLRETRTRFAPSPTGYMHIGNLRTALYTYLIAKHDGGKFILRI
                     EDTDQKRYVEDAVDVIYNTLNKVGLKHDEGPDVGGDYGPYVQSERRNIYREYAEKLVE
                     LDGAYYCFCSRERLDDLRQQAEGDRITFQYDGYCKSLSKEEVQEKLHAGEPHTIRQRI
                     PKTGKTTFEDAVYGTITVENSELDEGVLLKTDGLPTYNFANVVDDNLMGITHVIRGSE
                     YLSSAPKYNLIYGAFGWNIPTYVHLPLIMKEGGRKLSKREGDASFEDFYEKGYLPEAI
                     INYIALLGWSPGTEQEFFTLDELVEQFDIKGLSKSPAVFDVNKLRWMNGEYIRKMDLE
                     DFHESALPYYKKYISSADVDLSLLSKLLHTRVDALEDIGDHVDFIEALPQYDIDLFIH
                     KRMKTTHGNALASLKKAYEVLADLEDWGDEAVHKVLFDTIKEMGIKNGQMLWPIRTAL
                     SGKPSSPGGGTDLLVLLGRKESLKRIKTGIKKLGIIK"
     gene            3552..4337
                     /locus_tag="VFD89_06480"
     CDS             3552..4337
                     /locus_tag="VFD89_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZJ57872.1"
                     /translation="MRIYSWNVNGIRAVQKKGFIEWVKEEQPDILCLQETKAHLSQLD
                     DELINIEGYESYWNYPERKGYSGVALYTKTEPKSVYYGIGIDIYDEEGRIIVAEYDDF
                     VLLNIYFPNGQMSDERLKYKLDFYDAVLKYCNMRVAQGDKVVVSGDYNTAHSEIDLKN
                     PKSNADRSGFLPIERAWMDKLVKSGYTDTYRYLYPDTVKYSWWSYRFRSREKNIGWRI
                     DYHFVSNNMLDRVKDSLILNHVMGSDHCPIMLELIPKGNPSGK"
     gene            complement(4512..4946)
                     /locus_tag="VFD89_06485"
     CDS             complement(4512..4946)
                     /locus_tag="VFD89_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HZJ57873.1"
                     /translation="MGNRTDSQGIYELVEKYRNAKFLHNGRSLDEGIDCLGFIILFYR
                     DLGVNLPNDDGKPIEEDWFKTNPNRYIRAIKNLGYKEVRFRDLRPLDMVYFAISRNII
                     THTGIMISKREFAHMSPNKNFLISKLERGWLSRARGAVRVIE"
     gene            5372..5575
                     /locus_tag="VFD89_06490"
     CDS             5372..5575
                     /locus_tag="VFD89_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57874.1"
                     /translation="MGFGFGHGGGRGADNSLLFFFLLLVIIFGNCGLFDDGSELLFFF
                     LLLVILFFGENGIGFFGVEAEIE"
     gene            5776..6006
                     /locus_tag="VFD89_06495"
     CDS             5776..6006
                     /locus_tag="VFD89_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57875.1"
                     /translation="MGYGFGASGHGKGGFDQSMIIWILVIFLLMGDGGFNDFGKGCFG
                     KGDDYSILIIIIVLLLFSGGGGFLGLGAPTEE"
     regulatory      6343..6449
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            6608..7903
                     /locus_tag="VFD89_06500"
     CDS             6608..7903
                     /locus_tag="VFD89_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HZJ57876.1"
                     /translation="MKRDYEGHFDTIAVHGGQTPDSDTGSRAVPIYQTSSYVFDSVEH
                     AANLFALKEGGNIYTRIGNPTTSVLEERLAMLEGGVGALATSSGQAAITLAILNITGS
                     GHNFISSKYIYGGTHTLFLHTLGRLGIEVRFADLSDPNECRGLIDENTRFIYTESIGN
                     PKNNVDDFKALAELAHSHGIPFIVDNTVTSPYLFRPFEYGADIVVHSLTKYIGGHGTS
                     IGGAIVDSGKFDWSDPKFAELNQPDPAYHGLNYWEAFKETGAAYITKARVQLMRDLGP
                     SLSPFNSFLFLQGLETLPLRMKRHCENAMKASVWLKSHPAVSWVNYPGLEEHKDFDNA
                     KRYLPTGQGAIVGFGIRGGKPAGIKFIESLDLISHLANIGDAKSLIIHPASTTHQQLT
                     PKEQEESGITDDFIRFSVGLEDIDDILTDLDQALIKSQG"
     gene            8360..9586
                     /locus_tag="VFD89_06505"
     CDS             8360..9586
                     /locus_tag="VFD89_06505"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZJ57877.1"
                     /translation="MKNVNKKSDSKSKKKYETPDSPWSVGWTSPHVVTLADEDNPLLL
                     DCGEELAPISVEYEVYGEMNPRRDNVILLLHALSGDAHAAGWDKEAQDMGRSWRLSRP
                     GWWDTMIGPGKALDTNIYCVICSNVLGSCYGTTGPVSIDPDTGKPYGLDFPIVTVGDW
                     VRLQERLVSYLGIEKLYAVVGGSLGGQQALEWSLAYPDRVERAVILASSARLSSQGLA
                     FNAVARNSILNDINFNGGDYYEGDQPAQGLAVARMLGHITYLSETSMRDKFGRRYRKN
                     GDRPGFHLGVDFEVESYLEYQGQSFVSRFDANSYLYITRAMDYYDAADRGGGDLDKAC
                     KTAKARFLLISFSSDWLYTPEQMKELTFALYRTKKDVSYVEIPSTYGHDAFLLEVDRI
                     TPLISKFLKGVACHGV"
     gene            9576..10187
                     /gene="metW"
                     /locus_tag="VFD89_06510"
     CDS             9576..10187
                     /gene="metW"
                     /locus_tag="VFD89_06510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HZJ57878.1"
                     /translation="MEFELQQDMRWDHKVIFDLVTDGASVLDLGCGNGDLLKKLIEQK
                     NVWGMGVERNMERVAGCIENSVPVYHADLDHGLPQLPGHFFDFVILEKTLQAVASPMG
                     LLDEMLRLGKVGIISFPNFGYKEVVASFVQSQRMPRTEALPYYWYNTPNIHLFTARDF
                     LDLVKERDLKIEMGVSWIRGETRPFSEEESDEAEEILFMISRR"
     gene            10368..10946
                     /locus_tag="VFD89_06515"
     CDS             10368..10946
                     /locus_tag="VFD89_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZJ57879.1"
                     /translation="MQVLKDEVRNNLIEAATEEFLAEGYNNSSLRIIASKAGMTIGNI
                     YSYFSSKDNLFETVIDPALKGLKSLISLDVTGYESKDQPSLIEITEEICQVFMANKRG
                     FLILMNGSKGSQFKDIRQDIIDFISDRIEREIYPRASSAQMDPLFAKALASGIVSSFI
                     TVFNNYGGDEGRLFYLVNELIYVVLGNIESRL"
     gene            11013..13469
                     /locus_tag="VFD89_06520"
     CDS             11013..13469
                     /locus_tag="VFD89_06520"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZJ57880.1"
                     /translation="MKKKRYRLLESIAEVIIDRRNLFFLFYIITIIFSLFSMGWVDVE
                     EDIANYLAEGSKTRQGITIMEEEFTTYGMANVMVSNIAPTNAMNIARGIEEIDGVDSV
                     MFENNEDHYRDTSALFIITFLGEETDEITLQAMDEVRNLISPYDFSVSTTIGSSIASQ
                     LTKDMTLIGILAGIIIVIVLLFTSKSYAEVPVLLITFGVAALLNMGTNFLLGKISFIS
                     NSVAVVLQLALAIDYAIILCHRFSEENEIHPPREAAIVALRKAIPEISSSSLTTIAGL
                     GALAFMEFGIGKDLAMVMIKAILLSMLSVFTLMPGLLVLFSGLIHRSRHRSFVPSVSA
                     LGRFSIKTRYIIPPIFALVLVVAFILSAKCPFVFSLSGIRAQRLSEAQIAEDRIEEKF
                     GSSNMVALMVPSGNYEGEKKLLRILEGYEEVDKAIGLANTEAIGGYMLTDILTPRQFS
                     ELMDLDYEVAQLLYTAYAIGDEDYGKVISGIGQYGIPLIDMIQFSYDQVKGGFVTLDG
                     ELMEELEDSNSQLSSARNQMESEKFSRMFLALNLPGEGQETFDFLTMIQKEAAKYYDK
                     GSIYLAGESTNSWDLSSTFDRDNIIISILSVLFVVIILTFTFQSAGLPFLLISVIQAS
                     IWINFSVPFIRNQGLYFLGFLLVSSIQMGANIDYAIVMSSRYLSLRENMSRDKAVIMA
                     LNQGFPTVITSGTILAVAGILIGQISTDGATSVLGSYLGYGTIISMILVIFVLPQLLY
                     LGDIMVEKTSFRLKLLTSRYQASRGKVQLRGKLKGYVDGEIDAYVEGSITGRIQGTFD
                     IDEEPKEDLEGETGDAYEEV"
     gene            13450..16662
                     /locus_tag="VFD89_06525"
     CDS             13450..16662
                     /locus_tag="VFD89_06525"
                     /inference="COORDINATES: protein motif:HMM:NF019217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HZJ57881.1"
                     /translation="MHMRKFSTILAVIIILSTLIPDVIGASPEPAYRIISIDDAKDLR
                     KLSEDCSYDAFSRGIKVILNADIDLKDEDFIPIPIFAGIFDGGGHTIKGLSVAVEGSN
                     QGLFRYLEEDATIRDLKVQGAVTPTGEKSTIGGLVGYNQGTIENCEFSGYIKGKDIVG
                     GLAGWNGSTGMITDSWTKGAVYGRRKVGGIAGYNTGTILSCTNESSVNTTIEEYKVDL
                     GEIDIENLDMAELSKLSSDITDIGGISGINTGTIKNTQNHGTVGYPQVGYNIGGIAGR
                     QTGYISGCLNQGIVQGRKEIGGIVGQMEPYIDVLIPPSKLNKLHEELNTLHSSINKMI
                     SFTQTTSADMNQKLSKIQDGINAGKAHAQELISMSEDLIDKTGTAGEEILGRIKPIIE
                     GLNDIPKDLENAISTMEESMDYIRRILEKIEEYDWDDERLTLKAEHLLSIAKDVRDGL
                     GRAGADIDQVLKKLQTGETEGIPELLQSVSESLNSVMDVVSEGLEDIREIGEIIGELI
                     VSLDGDISEDLKAALDYMDRAFDLLGDVFNKIQDISKQINDLIGYFTDMPQLDFEAIR
                     DVYEKTKEDLFDSMGDMSVTLSDLMDSMFVQGKIMIGDMQGINDQLFLVMDLSFSLID
                     DLMNEEKGLDNVYQDVSREDIDKKTDGKVFRSKNLGTIEGDINVGGIAGAMAIELKFD
                     PEDDFNIIKNTPINSVFQTSAIIQQCENGGGVVGKKNNVGGIVGSMDLGYILDGVAAD
                     LVESTDGDYVGGIAGKAHGPIVNSYAKSTLRGANYIGGIAGYGTEILDSHTLIRIERS
                     NACVGAIAGDVDKNNVIKANSFVSKTLSGIDGISYIERAEPISYRELISKENLPSIFK
                     EFRLAFVAEDEIIRTQNLNYGDSVSKIDLPDVPEKDGYHGEWEEFDGEDITFDTTIKA
                     IYTPIATVLESDEKWDNVLPMVLVEGQFAYDDTLTLGFDDEAELPEFKKGAEADQLTV
                     IIPDDGESKHIIRYLPSVAGREMDLYILSSGNWSRSKSHTDGKYLVFEAEGNTVTFAV
                     VYNKLPYKGLIVLVAVISIVVVLTIYVKNRKRHPKSIGLG"
BASE COUNT         5131 a         3284 c         4244 g         4531 t
ORIGIN      
        1 catatatagt tcattatcca aatgatagac tattgaaaag cggatgactt cattagttat
       61 gataccatat tgcatagaaa attaacagac ccgggaaatt tcgaaaccac caaaaattcc
      121 taagttgatg aaaaaattga aaggtgataa aataagaaat aatggggaaa ccggaccttt
      181 atccattgga acaagggatt aaggcaggat atttatatcg gttgattttg aagaaacgat
      241 ttatgtcgtg taatacatgg aacataatat ttggcatagg tccgtagcat aacgctttaa
      301 ataaaaagta aggagaatgc aatatatgag caacaacaaa gaaaatacca aagtggaggt
      361 aaatataggt agtaacttta tccacgatat catcgacagg catttagagg atggggttta
      421 taaaaatgta cacactcgat tcccccccga accaaatgga tatctgcata tcggacatgc
      481 caaatccata tgtctaaact tcgggacagc ggataaatat aatgggctat gcaatctgcg
      541 ctttgatgat actaatccct tgaaggagga agtagaatac atagattcta ttatcgagga
      601 cgtaaggtgg ttgggattcg attgggaaga tagactgtat tatgctgcag attattttga
      661 taagatgtat gaatatgccc tccttctcat aaaaaaggga aaggcatatg tagatgacct
      721 gactgctgag gagataaggg aatacagggg gacccttacc caaccgggga agaacagccc
      781 ctacagggac cgaggggtgg aagagaattt ggatctcttt gagcgaatga aggcagggga
      841 atttgaagat ggttcaaggg tattgagggc aaagatcgat atggcttccc caaatatgaa
      901 tatgcgagat ccggtaatat ataggattgc ccatgttccc catcatagaa gcggggataa
      961 atggtgcata taccccatgt atgactatgc tcatcccata tccgatgcat tggagaatat
     1021 tacccattcc atatgtactt tggaatttga ggatcataga cctctttacg aatgggtttt
     1081 agatgaaata gacctttttc caaatccgcc gattcagata gagtttgcca ggctggaact
     1141 caccaacacc gttatgagca agcgaaagtt aagggtattg gtagaagagg gcaaggtagc
     1201 agaatgggac gatccaagga tgcccaccct atcggggctt cgccgaaggg gatacactcc
     1261 ctcttctata agggattttt cggaaagggt tggaatagcc aagaacaaca gcgtggtgga
     1321 tgtcgccctt ttagagcatt gcctaaggga agagctcaac agggatgccc aaagggtgat
     1381 gggggtatta gatcctataa agctggtaat aaccaattat cccgaggacc aggtagaata
     1441 tatggaggtg gagaacaacc aggaggatcc gtccactgga accagaatgg tgccgtttgc
     1501 tagggaacta tatatagagc gtgaagactt tatggaagat ccacccaaga agtttttccg
     1561 gttgactccg ggcaaggaag tgcgcctaaa gaatgcctat ataattcgct gtgagggcgt
     1621 tatcaaggat caggagacgg gcgaaatttt agaggtccat tgcacctatg atccggatac
     1681 caaaagcggt tcggggtcgg ctaacagaag ggtcagaggt actctacact gggtgtctgt
     1741 agtccatgga atacccgtta tgatcaggct ctatgattat ctatttacag aggatgaaga
     1801 tgggggaatg cagtataacc cggattctat aaaggaactg aaaggctacg tggaaccctc
     1861 attggagggt accgaggggg gcagtcgatt ccagttcttc agacagggct acttctgtac
     1921 cgatataaag gattcaacgc cggacaacct ggtgttcaac caaattgtgc ccctaaagag
     1981 ttcctatagg cccaatagat aaaaagggct ttaaacatcg aacctaattt taacataaga
     2041 tctgattacg taatttatcc gggaggttat aagattgagg gaaacaagaa ctaggtttgc
     2101 ccctagcccc acgggctata tgcacatagg caatctgagg actgcattgt atacctatct
     2161 gattgcgaag catgacggag gtaaatttat actcaggatt gaggatacgg accagaaaag
     2221 gtacgtagag gatgcagtgg atgtcatata taatactcta aataaggtgg gccttaaaca
     2281 cgatgaaggc ccagatgtag gcggtgatta tgggccatac gtacagagcg aacgccgaaa
     2341 tatttacagg gaatacgccg aaaaattggt tgagctagat ggcgcatatt attgcttctg
     2401 cagcagggaa aggcttgacg atttgagaca acaagcagaa ggggatagga tcacatttca
     2461 atacgacggg tattgtaagt ccctcagtaa ggaagaggtc caagagaagc tgcatgcagg
     2521 ggaacctcat acgatcaggc agcggatacc taaaacgggg aagaccacct tcgaagatgc
     2581 agtatatggg accatcaccg tagagaattc ggaactggac gaaggcgttc tcttaaagac
     2641 cgacggtctg cctacctaca attttgccaa cgtagtggac gataacctta tgggaataac
     2701 ccatgttata agagggagcg aatatctctc ttccgctccc aaatacaact tgatctatgg
     2761 cgcctttggt tggaatatcc caacctacgt tcatttgccc cttatcatga aggagggggg
     2821 cagaaagctc agcaagaggg aaggggatgc ctcctttgag gatttctatg aaaaggggta
     2881 cttaccggaa gctataatta actatatagc cctgctgggc tggagtccgg ggacagagca
     2941 ggaatttttc accttagatg agcttgtaga acaattcgat ataaagggac tgagcaagtc
     3001 cccggcggta ttcgatgtca ataagctcag gtggatgaac ggagaatata taaggaagat
     3061 ggacttggag gattttcatg agtcggctct gccctattat aaaaagtata tctcatctgc
     3121 ggatgttgat ctgagccttc ttagcaagct tttgcatacc agagtagatg ccttagagga
     3181 tataggggat catgttgatt ttatagaagc attaccccaa tacgatatag atctttttat
     3241 ccataaaagg atgaagacta cccatgggaa tgctttggcg agcctaaaaa aggcatacga
     3301 agttcttgcc gatttagagg attggggaga cgaagccgtc cacaaggtat tgttcgatac
     3361 aataaaggag atggggatta aaaatgggca gatgctgtgg ccgatacgga cagcactatc
     3421 cggcaaaccg tcgtctccgg gcggaggcac cgaccttttg gtgcttctgg gaaggaagga
     3481 atcccttaaa aggataaaga cagggataaa aaagttgggt atcatcaaat aaagggcttt
     3541 ggggtgagtt tgtgagaatt tattcttgga atgtcaatgg gataagggcg gttcaaaaaa
     3601 aggggtttat tgagtgggta aaagaggagc agccagatat attatgcctt caagaaacta
     3661 aggcccacct gtcccaattg gacgatgaac ttataaatat agaaggctat gagtcctatt
     3721 ggaactatcc cgaaagaaag ggatatagcg gtgttgccct gtacaccaag acggagccta
     3781 aaagcgtata ctacgggata ggcatagata tatatgacga agagggcaga ataatcgttg
     3841 cggaatacga tgatttcgtt cttctcaaca tatattttcc caacgggcag atgagcgacg
     3901 agaggcttaa gtataaacta gatttctacg atgcagtact aaaatactgc aatatgaggg
     3961 tagcccaggg ggacaaggtc gttgtttcgg gggattataa taccgcccac agtgaaatag
     4021 acttaaaaaa ccccaaatcc aacgcggatc gttcgggatt tttacctata gagagggctt
     4081 ggatggataa gttagtaaaa agcggataca cggataccta tcgatacctc taccccgata
     4141 ccgtaaaata ttcctggtgg agctatagat ttcgatccag agaaaagaac atcggctgga
     4201 ggatagacta ccactttgtt tcaaacaata tgctggacag ggtaaaggat tcactaatat
     4261 taaaccatgt aatgggttcg gatcattgtc ctattatgct ggaattaatt ccaaagggta
     4321 atccaagcgg taaatagata tgctgcgata gaggcctttc attacaggtt cagttaaatt
     4381 atctgatcca ctgttaaggc aatgtacttt ccgcaacata tgaattgagg ataatatctt
     4441 acgacatttg tatgtcggtg cagaatgttt gccgtaaaat gtaaggatag ggcaggaata
     4501 aaagcattcc cctactctat aacccttacg gcccccctag ccctggagag ccagcccctt
     4561 tctagcttgc ttatgaggaa gttcttattt ggggacatat gtgcaaactc ccttttactg
     4621 atcataattc cggtatgggt aattatatta cggcttatgg cgaaataaac catatccaac
     4681 ggcctaagat cccggaatcg aacctcttta tatcccagat tttttatagc cctaatatag
     4741 cggttgggat tggtcttaaa ccaatcctcc tctatgggct tcccatcgtc attaggcaga
     4801 ttcacaccaa gatccctgta gaagagaatt ataaagccca ggcaatcaat tccctcatcc
     4861 aaggatctac cgttgtgaag gaacttggca tttctatact tttcaaccaa ttcatatatc
     4921 ccttgactat ccgttctgtt tcccatggat ttacccccta ttatcaatgt ttatgcccgg
     4981 ccaaatatct aaagatatcg tggcaatcgc atcatatgat tttaacgaca tgacagtccc
     5041 tgcacgcaat ttatttagcc tatcggaata tctaatccat ggtgaaggta ggtatcattg
     5101 cgcgtcgtaa tattcataat atggagttaa atataaaggg tgataaggac cccgaggatt
     5161 ttatcacatt aaaaatcctc ttggcataat atatagtgat gttatagatg agcaatttag
     5221 gctgctacag tttcacatag ggtgcgttat ttaatgtaaa tgccttaacc cggtttaaca
     5281 ctttataacc accatgtgta tgattagccg gcataatcat tatacgtagc ttttgtctat
     5341 agttgtatct attcaaggga ggagtgaaga aatgggattt ggtttcggtc atggcggagg
     5401 aagaggcgca gataactcac ttctattctt ctttttgttg ctggtaataa tatttggtaa
     5461 ctgcggctta ttcgacgatg gaagtgaact attattcttt ttcctgcttc tggtgatatt
     5521 gttttttggg gaaaacggca taggcttctt tggagtagag gcagaaatag aataatggga
     5581 ggcaaaaaat gctgttcctg ctcttatggg gacagcattt ttaaatttcc tcacacattt
     5641 taggtgcggc gggaatagta tataagtatc ctatgtactt actaaggata aacaactcca
     5701 actataaaat ctatcatggc gataaatttt ggttccgtat tgtttatcta caaataaatt
     5761 aagagaggtg ctggtatggg atacgggttt ggcgcaagcg ggcatggtaa gggtggtttt
     5821 gaccaatcca tgatcatttg gattctagtt atttttctat tgatgggaga cggaggtttt
     5881 aacgacttcg gcaagggctg tttcggaaaa ggggacgatt actccatact tattatcata
     5941 attgtgctcc tgctctttag tggcggcggc ggatttttag gtttaggggc ccccacggaa
     6001 gagtaattta ttctttcctt aaggaatttt ataacatgat gggttttaac atgtgtttca
     6061 gcgttgatac tatgaacctt tagcaacaat ggattgggga taataccagg atataagtgt
     6121 gggatgctcg atgtaagcgt acggtaagaa cggtagggcc cggataaaaa agatggcatg
     6181 ttattttact gccatctttt ttggaattat tccccggcac tgcacatcga gaatgtaata
     6241 tatccgctat gctgacatta ccccttgtta aaaacaaaac tattgttgac atgccggtga
     6301 taagtcgtat ataatgcata caattaaaaa catataaata acttcttatc cagagggggg
     6361 gagggactgg ccctatgaac cttcggcaac cttcagagcg tatctgaaaa ggtgccaatt
     6421 ccagctgata tagatcagac agataggagg aggataattg gcattgtagc ccttctttct
     6481 gtaaccgaaa ggagggcttt tgagtatctt tatcccaata cctctatgcc gaggtaacca
     6541 agccagtatt cacggattcg attattaatg caatgtattc aatattcaaa cgaaaggaat
     6601 gatagatttg aaaagggatt acgaaggaca ttttgatacg atagctgtgc atgggggaca
     6661 gactccggat tcggataccg gatccagagc cgttcctata tatcagactt cgtcttatgt
     6721 atttgattcg gtagaacatg ccgctaatct ctttgccctt aaagagggcg gcaatatata
     6781 cacccgtata ggaaacccta cgacttctgt acttgaggaa cggctggcca tgctggaagg
     6841 cggggtagga gcgctggcta cctcatcagg gcaggcggct atcaccttgg ctatactcaa
     6901 cataaccggt tcggggcaca attttatatc atcaaagtac atctacggcg gaacccatac
     6961 cctctttctg cataccctgg gtagactggg catcgaggta cggtttgcgg atctgtcgga
     7021 cccgaacgaa tgccgtgggc tgattgatga aaataccagg tttatatata cggaatccat
     7081 aggaaacccc aaaaacaatg tcgacgattt taaggccctg gcggagttgg cccatagcca
     7141 cggtatcccc ttcatagttg ataataccgt gacaagcccc tatcttttcc gtccctttga
     7201 atatggtgca gatatcgtag ttcattccct gacaaaatat atcgggggac atggaacatc
     7261 cataggcggg gccatcgttg attctgggaa attcgattgg agcgatccca aatttgcaga
     7321 actcaatcaa cccgatcccg cataccatgg actaaactac tgggaggcat ttaaggagac
     7381 gggggctgcc tacataacca aggcaagggt ccagcttatg agggatttgg gaccgtccct
     7441 ttctcctttc aattcatttt tgttcttaca gggtctggag accttgccac ttcggatgaa
     7501 gcggcattgc gaaaatgcta tgaaggcatc agtgtggcta aaatcccacc cggcagtatc
     7561 ttgggtcaac taccccggtt tggaagaaca taaggatttt gacaatgcaa aaaggtatct
     7621 ccccacgggt caaggagcca tagtcgggtt tggtataagg ggcgggaaac ccgcgggaat
     7681 caagtttata gagtccctgg acctaatatc ccacctggct aatatagggg acgcaaagag
     7741 cttaattata catccggctt ccaccaccca tcagcagttg acacccaaag aacaggaaga
     7801 atcggggata accgacgact ttatcaggtt ttccgtgggg ctggaggata tagacgatat
     7861 attgacggat cttgatcagg cgctaattaa gagtcagggc taacggtttc gcttaggcga
     7921 tcattccgtc atacagctgc ttatggggtt taactgggcg ttcaacagct attatcgaat
     7981 taaggctgat ccgtgcaatg gattaattag gcagattcgg tataacgagt cggtatgatg
     8041 tccccccgct tctataagcg acgagtaagt tccgattagt aaaacatgcg gtggatataa
     8101 atcacccgcc tcgttgaaac ctgtgtgttg caattgccaa tgcaattgct tccgatgtta
     8161 taacgagtcg gcaagatgtc cccccgcttc tataagcgac gagtaagttc cgattagtaa
     8221 aacatgcggt agatataaat cacccgcctc gttgaaaccc gtgtgttgca attgccaatg
     8281 caattgcttc cgatgtttta accgagatag tatatccaaa gaaaatagtg cataggcgta
     8341 cttaaggaga gactaggaat tgaaaaatgt caacaaaaag agcgactcaa agagtaagaa
     8401 aaaatatgaa accccggatt ccccatggtc tgtggggtgg acaagtcccc atgtcgttac
     8461 cctggccgac gaggacaatc cccttttgtt ggactgcgga gaggaattgg ctcctatatc
     8521 tgtagagtat gaggtgtacg gggagatgaa tccccgtaga gataatgtta tattgcttct
     8581 ccatgcccta tccggagatg cccacgccgc aggctgggat aaagaagccc aggatatggg
     8641 ccgcagctgg agactcagca ggccggggtg gtgggacacc atgatagggc ccgggaaggc
     8701 cctggacacc aatatatatt gtgtaatttg ttccaacgta ctagggagct gttatggaac
     8761 cacggggccg gtttccatag atccagatac gggtaaacca tatggcctgg atttccctat
     8821 tgttacggta ggagattggg ttaggctgca ggaaagactg gtttcatatc tcggaattga
     8881 aaagttgtat gcagtggtgg ggggctccct aggtgggcaa caggcccttg aatggagcct
     8941 ggcatatccc gatagggtag aaagggccgt tattctggct tcttccgcca ggttaagttc
     9001 ccagggactt gcttttaatg cggtcgcccg caattctata ttgaatgata taaactttaa
     9061 cgggggagat tactatgagg gcgatcaacc cgctcaaggc ctggctgtag ccagaatgtt
     9121 agggcatata acctatcttt cagaaacatc catgagggat aaattcggca gaaggtatcg
     9181 caagaacggt gatcggcctg gatttcattt aggggtagat ttcgaggtgg aaagctatct
     9241 cgaatatcaa gggcaatcct tcgtatccag gttcgatgcc aacagttatc tgtatataac
     9301 aagagcaatg gattattatg acgctgcaga tagaggggga ggggacttag acaaggcctg
     9361 taaaactgcg aaggcgaggt ttctcctaat atccttttct tcggattggc tctacacccc
     9421 ggagcagatg aaagagttga ccttcgcatt gtacaggacc aaaaaggacg tatcctatgt
     9481 cgaaattcca tcgacttatg gtcatgatgc cttcctttta gaggtggacc ggattacgcc
     9541 ccttatatct aaatttttaa agggggttgc atgtcatgga gtttgagtta cagcaggata
     9601 tgaggtggga ccacaaggta atatttgatc tggtaaccga tggagcttcg gtcctggatc
     9661 ttggatgtgg aaacggggat cttttgaaaa aactcataga gcaaaagaat gtatggggta
     9721 tgggtgttga aagaaatatg gaaagggtgg caggctgcat cgaaaatagt gtaccggtat
     9781 atcacgcgga tctcgatcat ggactacccc aactgcccgg acattttttt gattttgtaa
     9841 tcttagagaa gaccctacag gcggtagcaa gccccatggg gctattagat gaaatgctga
     9901 ggctaggaaa ggtgggcata atcagtttcc caaactttgg gtacaaagag gtggtagcat
     9961 ccttcgttca aagccaaagg atgccccgca ctgaagccct tccctattat tggtataata
    10021 ctccaaacat ccacctcttc acagccaggg atttcttgga tttagttaaa gagagggacc
    10081 tcaagattga gatgggtgtc tcatggataa ggggtgagac aagacccttc tcggaagaag
    10141 agtcggatga ggcggaggaa atactgttta tgatatcaag aaggtgaaac ttacttatta
    10201 aagggcggcc gatggccgtc ctttaatgtt ttcataagaa aattagtccg cttaagatcc
    10261 tgcctaccga aatatttaag tataccttat ccaaaaccaa aaagatcatt gacatatggg
    10321 aataatgcat tagaataaag ataataccac taagcaggtg attagtattg caagtactaa
    10381 aagatgaggt cagaaataac ttaatagagg ccgctactga ggagtttctg gcagagggct
    10441 ataacaactc ctccctccgt atcattgctt ctaaggcggg gatgactata ggtaacatct
    10501 attcctattt ttccagtaag gataacttat ttgaaaccgt cattgatccg gctttaaagg
    10561 gtctaaaaag cctaatctct ttggacgtta cggggtatga aagcaaggat cagcccagct
    10621 tgattgagat aaccgaggag atctgccaag tatttatggc aaataaacgg gggtttttga
    10681 tccttatgaa cggtagtaag ggctctcaat ttaaagatat cagacaggac ataatagatt
    10741 ttatcagcga tagaatagaa agggagatat atcccagggc ctcaagcgca cagatggatc
    10801 cgttatttgc aaaagccttg gcatccggaa tagtcagcag ctttatcacc gttttcaaca
    10861 actatggggg ggacgagggg agactattct atttggtcaa cgaactgata tacgttgtct
    10921 tgggtaatat cgagagcaga ctttaaatag atgcattatg tcgactcttt accggtatgt
    10981 attatttgaa atatcctgat aggatgggga agatgaaaaa gaaaagatat agattgttgg
    11041 aaagcattgc ggaggttatt atagataggc ggaacttatt tttcttgttc tacatcatta
    11101 cgatcatctt cagcctattt tctatgggct gggtcgatgt ggaagaggat attgccaatt
    11161 atttggccga gggcagtaag acacgccagg gaataacgat tatggaagag gaatttacca
    11221 cctatggcat ggcaaatgta atggtttcga atattgcacc taccaatgct atgaatattg
    11281 cccggggcat cgaagaaata gacggggttg attctgtaat gtttgaaaat aatgaggatc
    11341 attacaggga tacatcggct ctgtttatta taaccttttt gggcgaggaa acggacgaga
    11401 ttacccttca agctatggat gaagttagga acctcatatc cccttacgat ttttctgttt
    11461 ctacgactat cggctccagt atcgcttctc aattgacaaa ggacatgacc ttaatcggta
    11521 tccttgccgg catcatcatc gtaatcgtgc tcttatttac atcaaagtcc tatgccgaag
    11581 tgcctgtact ccttataact ttcggggtag ccgccctttt gaatatgggg accaattttc
    11641 ttttgggtaa gatctccttt atatccaact cggtagccgt ggtcctccag ttggccttgg
    11701 ccatagatta tgccattatc ctatgtcata ggtttagtga agaaaatgag attcacccac
    11761 ctcgggaagc ggccatcgtt gccctgagaa aagcaatccc cgaaatatcc tccagcagcc
    11821 tgacaacaat tgcgggtttg ggtgcccttg cctttatgga atttgggata ggaaaggatt
    11881 tagccatggt tatgatcaag gctatactgc tcagcatgct gtccgtattt acgctcatgc
    11941 cggggctttt ggtattgttt agcgggctta tacataggtc acggcatagg agctttgtac
    12001 cctcagtttc cgcattgggg cgattttcta taaaaactag atatatcatt ccgccaatct
    12061 ttgccctagt cctagtagtt gcctttatac tgtctgcaaa atgccccttt gtctttagcc
    12121 taagcggtat acgtgcccaa aggctttctg aggcccagat tgcggaagat cgaatagagg
    12181 agaagtttgg ttccagcaat atggttgcgt tgatggtacc ctccggaaat tatgaagggg
    12241 aaaagaagct cttaaggatc ttggagggat acgaagaagt agacaaggct atcggactag
    12301 ccaatacaga ggccattggc ggatatatgc tcacggatat actgaccccc agacaatttt
    12361 ctgagttgat ggatcttgat tatgaagtag cccaacttct ctacaccgcc tacgcaatag
    12421 gcgatgagga ttatggaaag gttattagcg ggatcggtca atatgggatt ccccttatag
    12481 acatgattca attttcttat gatcaggtaa aaggggggtt cgttacccta gatggtgagc
    12541 taatggagga gctggaagac tctaatagtc agctgtcgag tgcccgcaac cagatggaaa
    12601 gtgagaaatt cagcagaatg tttctggccc taaacctccc gggcgaaggg caagagacat
    12661 ttgattttct gactatgatt caaaaggagg ccgccaaata ctatgacaag ggttctattt
    12721 atctggcggg ggaatccacg aattcctggg acctgtcatc aacattcgac agggataata
    12781 tcataatcag cattttatcc gttttattcg tcgtcatcat tttgaccttc acgttccaat
    12841 cggcggggct tcccttttta cttatatccg taatccaggc cagcatctgg attaactttt
    12901 ctgtaccttt tataagaaat caggggctat attttttagg attcctgctg gtatcgtcca
    12961 ttcagatggg cgccaatata gattacgcca tagtcatgtc aagccgctat ctgtccctta
    13021 gagagaatat gtccagggac aaggctgtaa tcatggcttt gaaccaggga tttcccaccg
    13081 taataacctc cggaactatt ctggccgtag cgggtatact gataggccaa atttctacag
    13141 atggtgccac ttcagttttg ggatcctatt tggggtacgg taccattatc tccatgatac
    13201 ttgtcatatt cgtacttcca cagctgctct atctgggaga tatcatggtt gaaaaaacct
    13261 catttagatt aaaattactc acatcccgat atcaggcgtc gagggggaaa gtacaactga
    13321 ggggcaaatt aaaggggtat gtggatggtg aaatcgacgc ctatgttgaa ggctctatca
    13381 cggggagaat acagggaaca tttgatattg acgaagaacc gaaggaggac ttagaggggg
    13441 aaacagggga tgcatatgag gaagtttagt accattcttg cggtgattat aattctatcg
    13501 accttgatac ctgacgttat aggtgcatcg ccagaaccgg cgtacaggat tatttccatt
    13561 gacgatgcca aggatcttcg caaattatca gaggattgtt cctatgacgc cttctcccgc
    13621 ggtataaagg tgattctaaa tgccgatatc gacctaaagg acgaggattt tattcccata
    13681 cctatctttg cgggaatctt tgacggaggc gggcatacca ttaagggttt gtccgtagcg
    13741 gttgaagggt caaaccaggg gcttttcagg tatcttgagg aagatgcgac tataagggac
    13801 ttaaaggtcc aaggcgctgt cacccctaca ggggagaaat ctacaattgg cggcttggtg
    13861 ggctataacc agggcactat cgagaattgc gaatttagcg ggtatataaa gggcaaggac
    13921 atagtggggg gtctggcagg ttggaatgga agcaccggta tgattacgga ctcatggacc
    13981 aagggcgcag tatatggaag aagaaaggtc gggggcattg cgggttacaa cacgggtacc
    14041 atcctcagct gcaccaatga gtctagcgta aatacaacga tagaggaata taaggtggat
    14101 ttaggggaaa ttgatataga aaacctggat atggccgaat tatcgaagct atcatcggat
    14161 ataaccgata tagggggtat ttccgggatc aacaccggta ccataaaaaa cacgcagaat
    14221 cacggaacgg tggggtatcc gcaggttggc tataatatag gaggtattgc gggcagacaa
    14281 accggctata tatccggttg cttaaaccaa ggaatcgtac agggacgcaa ggagataggc
    14341 ggtatagttg gacagatgga gccatatata gatgttttaa ttcccccatc aaagctaaat
    14401 aagctccacg aagaactcaa cactctgcat tcttccatta ataagatgat aagctttaca
    14461 caaaccacct cagccgatat gaaccaaaag ctgtctaaga tacaagacgg tataaatgcc
    14521 gggaaagccc atgcacagga attaatcagt atgtcagagg accttataga taaaaccggc
    14581 acagcagggg aggagatact tggtagaata aagcccatta tagaaggatt aaacgatata
    14641 ccgaaagatc tggaaaacgc catatccacc atggaagaat ccatggatta catcaggcgg
    14701 atattggaaa agatagaaga atatgattgg gatgatgaga gacttacact gaaggcagaa
    14761 cacctcttat ctatagcaaa agatgtccgg gatgggctag gtagagctgg cgccgatata
    14821 gaccaggtgc tgaaaaaact tcaaacagga gaaactgagg ggatccccga attattgcag
    14881 tctgtatcgg agagccttaa ttcggtcatg gatgttgtat cagagggcct ggaggatatt
    14941 agggagatag gggaaatcat aggggagctt atagtctcct tggacgggga catcagtgaa
    15001 gacttaaaag ctgcgttgga ctatatggat agggctttcg accttctggg ggacgtattt
    15061 aataagattc aggatatctc caaacaaata aatgacctta taggctattt caccgatatg
    15121 ccccaacttg attttgaggc tataagggat gtctatgaaa aaaccaaaga ggatcttttt
    15181 gactccatgg gagatatgtc agtaacgctt tctgatctca tggatagcat gtttgttcaa
    15241 ggtaaaatca tgatagggga tatgcagggg attaatgacc agctcttttt agtaatggat
    15301 ctatcattta gcctgattga tgatttaatg aatgaagaaa aggggcttga taatgtctac
    15361 caagatgtat cccgagaaga tatcgataaa aaaacggatg gaaaggtatt ccgtagcaag
    15421 aacttaggta caatcgaagg agatataaat gtgggtggca tagcgggggc tatggctatt
    15481 gaattaaaat ttgatcccga agatgatttt aacataatta aaaacacccc cataaattca
    15541 gtgtttcaaa ccagtgcgat cattcagcaa tgtgaaaatg gcggaggggt agttggcaaa
    15601 aaaaacaacg taggcggtat tgtaggcagt atggatctcg ggtatatcct agatggtgta
    15661 gcggctgatt tggtagagag cactgacggt gactatgtag gcggcatagc aggaaaggcc
    15721 catggtccca ttgtaaacag ctatgcaaaa agtaccctaa ggggtgcgaa ttatataggc
    15781 ggtattgccg gatatggaac cgaaatacta gattcccata cccttataag aattgaaaga
    15841 tccaacgcct gtgtaggagc tatcgcaggt gatgtggata aaaataatgt cataaaggca
    15901 aattcttttg taagtaaaac cttatcgggc atagacggta taagctatat tgagagggct
    15961 gaacctatca gctacagaga acttatatca aaggagaatc tcccatccat ctttaaggaa
    16021 tttcgcttgg cttttgtggc cgaagacgaa ataatccgta cccaaaatct taattacgga
    16081 gactccgttt ccaaaattga tttaccggat gtaccggaaa aggatggcta ccatggggag
    16141 tgggaggaat ttgacgggga ggatatcacc tttgatacca ccattaaagc aatctatacc
    16201 ccaattgcaa cggttctcga aagtgatgag aagtgggata atgttttacc tatggtcctg
    16261 gtggaaggac aattcgcata tgatgatacc ttgaccttag gatttgacga cgaggccgaa
    16321 ctgcctgaat ttaaaaaggg agcggaagct gatcaattga ccgtaattat tccggacgac
    16381 ggggaatcca agcatattat caggtatctt ccatcggtag caggaaggga aatggatcta
    16441 tatatcctgt cgagcgggaa ttggagcaga tcaaagagcc atacagacgg gaagtatctg
    16501 gtatttgaag ctgaaggaaa taccgttaca tttgccgtgg tatataataa gctcccatat
    16561 aaaggtctga tagtcctagt agcggttata tctatagtag ttgtgttgac catatatgtg
    16621 aagaatagaa agagacatcc gaaaagtatc ggactgggct aataatatgt aattagggcg
    16681 aagaaagctt cttcgccctg ttactaaaaa agtcatgata agccgggata caaccatcag
    16741 gcgaaaggcc atatccccta atcatatgtc ataattcaag catcaaaagg gcatggggca
    16801 aaagttcact accacatatg aactgagcag gtatggaccg aaccgctata ctcatcacta
    16861 tatatggtgt atcctgttgg aaaaatgtat gcttgatacc ccaatcgtgt tgtagtatac
    16921 gactcgatca aattaaatca tttgcccatg gaaggcatgg atactgtgat cgccatagtg
    16981 attgaattgg gttttatgaa tgaatttatt taaaaagaat aaaaaattat ggtgttgaca
    17041 tactataaat gctgtgttac tatataaatc gttgttcaaa agacatcgat tcaggtgctg
    17101 gaggacgtta accacaaatt ggaatcaaaa gaattagaaa aaatggttga caaagcatcc
    17161 gaaatttgat aacatagtaa gagtcgcccc
//
LOCUS       DASRNX010000163         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_404780,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000163 DASRNX010000000
VERSION     DASRNX010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404780"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..636
                     /gene="greA"
                     /locus_tag="VFD89_06530"
     CDS             157..636
                     /gene="greA"
                     /locus_tag="VFD89_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HZJ57882.1"
                     /translation="MMVNKETLLTPEGVTKLEQELNFLKTVRRRQIAERIKQAIAFGD
                     LSENSEYDEVKNEQAFIEGRIVTLENMLRNARIIDDEDISTDVVSIGSTVKILDIEFD
                     EVMEYTIVGSAEADPGQNKISNESPVGRALIGQQVNSIVDVSVPDGIIKLKILEIKK"
     gene            963..2459
                     /gene="lysS"
                     /locus_tag="VFD89_06535"
     CDS             963..2459
                     /gene="lysS"
                     /locus_tag="VFD89_06535"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZJ57883.1"
                     /translation="MNETREDGIAQENLNEILKVRRDKLVKLREEGRDPFKITKFNPT
                     YKSVTIINDYENLEGQQVVIAGRIMTKRVMGRASFAHILDSDGQIQIFVQINELGREA
                     YEDFKEIDLGDIVGVTGTVFKTKKGEISVKAKEITLLTKSLQPLPEKWHGLRDTDLRY
                     RQRHVDLIVNPDVRKTFIIRSKIIQGIRNYLDQRGFLEVDTPILHNTAGGATARPFIT
                     HHNTLDIDMYMRIATELHLKRLIVGGFDKVYEMGRIFRNEGMSVKHNPEFTTIELYQA
                     YADYEDIMDLTEDMISTVAKDVIGVTDIEYQGQPVDLTPPWNRLTMVGAVKKYTGIDF
                     DSFSSGEEAAIALKGIGIEISKAITWGEALNLAFEEKVEEQLVQPTFITEYPVEISPL
                     AKRMDRDPCLTYRFELFITGRELANAFTELNDPIDQRERFLDQARQREAGDEEAHMMD
                     EDFINALEVGMPPTGGLGIGIDRLIMLLADSASIRDVILFPTMKPRSH"
     gene            2505..3155
                     /locus_tag="VFD89_06540"
     CDS             2505..3155
                     /locus_tag="VFD89_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57884.1"
                     /translation="MYRVKLNRERLKNHIQYDWWKYILCIIATIAIWNVVTTVTKPKT
                     PADKKIEIFLIGDYMFDEPAAAVSEGILDDFPNLWEVNINNISLGGEDLQMDYMGRQK
                     LMVMLASQTGDIYAFEKDEFAQIAEQGAFISLDEFVEENGDLIGEDRLGKSKKMILEE
                     DVEPHYYGISMNGLELFMDTGYDVSDKVIGIMGYSDNPSMAVEVLKWILNDGVKKD"
BASE COUNT         1110 a          567 c          816 g          946 t
ORIGIN      
        1 gcttattgta ttgtaaccca atggagtctt aaaagccctt agaataaaag aaaaatatag
       61 gcatatatta tcatcatatc tcttgccgat gtgtagggac atttgtttaa atatcctaga
      121 tatagggaag gcgtaataaa ataaagggga gacttgatta tggtaaataa agaaactcta
      181 ttaactcctg agggtgtcac aaaactcgaa caggagctaa actttttaaa gacggtaagg
      241 cgaagacaga ttgcggaaag aataaaacag gctattgctt ttggggatct cagtgaaaat
      301 tccgaatacg atgaagtcaa aaacgaacaa gcctttatag aaggccggat agtaactctg
      361 gaaaatatgc tcagaaatgc aaggattata gatgatgagg atatttccac cgatgttgtt
      421 agtatcggtt ctacagttaa aatacttgat atagaatttg acgaggttat ggaatacaca
      481 atcgtaggat ccgcagaggc cgatcccggc cagaacaaga tatccaacga atcgcctgtg
      541 ggaagagcgt tgattggtca gcaggttaat tcaatagtcg atgtcagtgt tcccgatgga
      601 attataaagt tgaaaatatt ggagatcaag aaataagaag ggatcgctat cagactatat
      661 gcccataggg tatattttat ttgtagctga agggttagat gtggtattat taaacgagac
      721 taaacaatat ctccattact ttaaatggcg gatcctactg gcgaattaga ttatgggcaa
      781 aaggttccgc cttgttgaaa cggtgtatta taattactga agcaattgta ccggcattat
      841 gatccggacg gtttatcaat caccatttcc gtatgttttt agttatccgt tcctatgcgc
      901 tctatcggta atttggtccg ctggtcgcta tcctatatat agttggaaag gtatgaaagt
      961 cgatgaatga aacgagagaa gacggtattg cacaagagaa tttaaatgag attcttaagg
     1021 tcagaaggga caagcttgta aagcttcgtg aagagggcag ggaccccttt aaaataacaa
     1081 aattcaaccc tacatataag tccgtaacta taattaacga ttatgaaaac ttggagggtc
     1141 aacaggtagt aatcgcaggg cgaattatga ccaaaagagt tatgggtagg gccagctttg
     1201 cccatatcct agatagcgat ggtcaaattc aaatttttgt tcagataaac gagcttggca
     1261 gagaagccta tgaggacttt aaggagatag accttgggga catagtgggt gtaaccggga
     1321 cagtgttcaa gactaaaaaa ggcgagatat ctgtaaaggc aaaggaaatc actcttttga
     1381 caaagtccct gcagcccttg cctgaaaaat ggcacggcct tagggatacc gatcttcgtt
     1441 atagacaaag acatgtggac cttatagtaa atccagatgt taggaagacc tttatcatcc
     1501 gttctaaaat catacagggg attcgaaatt accttgatca aaggggattt ttagaggtag
     1561 atacccctat tttgcacaat actgccgggg gggcaacagc cagacctttc attacccacc
     1621 ataatactct ggatatagat atgtatatgc gcattgcaac agagttgcat ctgaagagat
     1681 tgatagtagg tggttttgat aaggtttatg agatgggtag aatctttcga aatgaaggaa
     1741 tgtctgtaaa acataatcct gaattcacta ctattgaact ttatcaggcc tatgcagatt
     1801 atgaggatat aatggatcta acggaggaca tgatctccac agtggcaaag gatgtaatag
     1861 gtgtaactga tattgaatac caaggacaac cggttgatct tactcccccc tggaatcgtt
     1921 tgactatggt tggggcagtt aaaaagtata ccggtattga ctttgattcc tttagcagtg
     1981 gggaagaggc ggcaatagcc ttaaagggaa ttggtataga gatcagcaaa gccattactt
     2041 ggggtgaggc attaaacctc gcctttgaag agaaggttga ggaacaatta gtacagccga
     2101 ctttcattac ggaatatccc gttgaaattt cacctttggc aaaaagaatg gatagggatc
     2161 cgtgcttgac gtatagattt gaacttttca tcaccggccg ggaactagcc aatgccttta
     2221 cagaattaaa cgatcccata gaccaaaggg aacggttttt agatcaggcc agacagaggg
     2281 aagcgggtga cgaggaagcg catatgatgg atgaagactt tatcaatgcc ctagaggtgg
     2341 gcatgcctcc caccggcgga ctgggaatag ggatcgatcg ccttattatg ttattggcag
     2401 attctgcatc aataagggat gttattttat ttcctaccat gaaacctagg agccattaac
     2461 aaaaaatatg ggctaagttt gtatatcgat aggggtgtgt tgatgtgtat cgcgttaaac
     2521 tgaataggga aaggttaaaa aaccatatcc aatatgattg gtggaaatat atcctatgca
     2581 taatcgccac tattgctata tggaatgttg tgacaactgt tacaaagccc aaaacgcctg
     2641 cagataagaa gatagagata tttttaattg gcgactatat gttcgatgaa cctgctgcag
     2701 ctgtatcgga agggattctc gacgatttcc ccaatctatg ggaggtcaat atcaataata
     2761 tttccctggg cggggaggat cttcagatgg attacatggg ccgacaaaag ttaatggtca
     2821 tgttggctag ccaaaccggg gatatatatg cctttgaaaa ggatgagttt gcacaaattg
     2881 ctgaacaagg tgcattcata tctttagatg agttcgtaga agagaatggg gatcttatag
     2941 gtgaagatcg cctcgggaag agtaaaaaaa tgatcctgga agaggatgta gaacctcatt
     3001 attatggtat ttctatgaac ggcctggaat tatttatgga caccggctat gatgtatcgg
     3061 ataaggttat agggataatg ggctacagtg ataacccatc gatggccgtt gaagtactaa
     3121 aatggatatt aaatgacggc gtaaaaaaag attgagttgg atttatgtcg ggtcctacaa
     3181 cccggttcga acaatgttat ttttagtttt tgttgacaac gtatttggga tatgctatca
     3241 tataattccc cttcaggggg gataacatgt ctaaaatctt ttgttgacat aacatatttg
     3301 ctgtgttact atataaatcg ttgttcaaaa gacatcgatt caggtgctgg aggacgttaa
     3361 ccacaaattg gaatcaaaag aattagaaaa aatggttgac aaagcatccg aaatttgata
     3421 acatagtaag agtcgcccc
//
LOCUS       DASRNX010000164        10462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_405340,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000164 DASRNX010000000
VERSION     DASRNX010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10462
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405340"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1556
                     /gene="amrA"
                     /locus_tag="VFD89_06545"
     CDS             126..1556
                     /gene="amrA"
                     /locus_tag="VFD89_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HZJ57885.1"
                     /translation="MSTRRIAMGRIQGVYLAPHPPIIVDEVGKGDREGARTTIDSMGI
                     LGREIKSKNPDTIIVISPHGPVFADGIGIMSEDILKGDFSRFGAPGVSMAFQTHGDLI
                     ERIIGEAQRIGILAIDLDRDMCSSYNISGTLDWGVLVPLYFVNQHFTGYRLVTMGVSQ
                     LPYHELYAFGIAMKRAIESQDKDVVIIASGDLSHRLKEDGPYGFHPAGVELDKTITSL
                     IEEGDVEGLLTIDPELISEGGECGLRSIIMALGALDGQAFRPEVHSYEGPFGVGYCVA
                     SFEPEDADSSRNLMDKLIENSRRSIRDTREKEDDYVRLARESLETYVRTGETMDLGDR
                     LPDSMLDNRAGTFVTIKQHGQLRGCIGTIEPSRESIAHEIVENAISAGTRDPRFFPVA
                     EEELDSLVYSVDILMEPEPAEGFGDLDIKKYGVIVSHGSRSGLLLPNLEGISSPQEQV
                     SIALQKAGIDPDEDYEMQRFEVKRHL"
     gene            1553..2554
                     /gene="amrS"
                     /locus_tag="VFD89_06550"
     CDS             1553..2554
                     /gene="amrS"
                     /locus_tag="VFD89_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZJ57886.1"
                     /translation="MKEALFYTRAEGGKLRCLLCPNVCIIEEGDRGSCNVRVNMEGRL
                     IAQGYGNISAIAMDPIEKKPLYHFYPGSNILSVGGLWCNLRCSFCQNWHIAHREGETQ
                     FISPESLITMAKEYGSIGIAFTYNEPIINYEYILDTGILAADHNLKIVLVTNGYILPE
                     PLEKLLPHIDAMNIDVKGFTEEYYREVCGGSLSPIKRAVELAAGHCHVELTTLVVGGV
                     NDGAQEMESLFRWIKGIDPYIPLHLSRYLPNYKMNLPGTEVETMMSLKALGDKYLNHI
                     YLGNMGGVDNNTYCPNCRELLIDRGHYRARAAGISQEARCSGCSSDTRRILGAWGDK"
     gene            2854..3702
                     /gene="cdaA"
                     /locus_tag="VFD89_06555"
     CDS             2854..3702
                     /gene="cdaA"
                     /locus_tag="VFD89_06555"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HZJ57887.1"
                     /translation="MISDLLQRLEPYLRFPDVIRHVIDVAVVAFVLYQLLLLIRKTRA
                     EQVLKGLVFLFFIYFVTKGLRLNTIAWILENTINFGITAGVIVFHPEIRRALEQIGRG
                     RIFDRTLLMRDEKDRDARSIVDGIVAAVANMSKTRIGALIVIEKNTGINEIIETGIWI
                     DARLTRELLENIFVPNTPLHDGAVVIRGNRVAAAGCFLPLTENPNLSKQLGTRHRAAL
                     GISESSDALAIIVSEETGVISMANSGRLTRYLDIDGLKNVLGSIYVKDSDIGPFSFIK
                     RRSKNG"
     gene            3695..4909
                     /locus_tag="VFD89_06560"
     CDS             3695..4909
                     /locus_tag="VFD89_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HZJ57888.1"
                     /translation="MGSFFERNTTLKIISIIIALILWGMTPNNRDPVRDMSFRDIPIK
                     IENQQKLSENGLMISSEMPDSYSFDIRAKGSMVKFLDRSKIVALVNLSDINKTGEQSV
                     SVELQGLPGTSNIEIKSAPDIKIDVERIVSKSIPVLPKIADEDSLELGKRFYEIEPRS
                     VEVRGPESLIATAAYGQVSVSLGDKDKKLERSSAVQLFNDEDEALDMGFITINPEYCV
                     VTIYPNKTVGIDAVITGKPADGFVVMGEEVKPREVTISGNPEVLEAMDSIQTEILDIE
                     GATSNIVKEVRLQQQEDIRVSPGEPSTVQILVRIEKIVDKQVSIEDVEFRNIAEGVEA
                     NVEEDGGDISLTIRGPQSLVNAFGPEDLRLYVDLNESSRGGRTYPILVDKLPSGLEIV
                     DIEPENLKVIFE"
     gene            complement(4911..5318)
                     /locus_tag="VFD89_06565"
     CDS             complement(4911..5318)
                     /locus_tag="VFD89_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZJ57889.1"
                     /translation="MNKLILKVLMVDNSYNARRDIDSKVKWVEHDFELVGEANNGEEG
                     LGLLPHVAPHVILTEIRMPKMDGITFIETAKKRWPNIKYVIVSNYDNFDYLRQAMKVG
                     AYDYILKPVKVEEINQVLLRAKTEIETGVRKFF"
     gene            5586..5661
                     /locus_tag="VFD89_06570"
     tRNA            5586..5661
                     /locus_tag="VFD89_06570"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5619..5621,aa:Arg,seq:ccg)
     gene            5828..7222
                     /locus_tag="VFD89_06575"
     CDS             5828..7222
                     /locus_tag="VFD89_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZJ57890.1"
                     /translation="MVPDKKFEIIALTMRYWFIGLIIFVVIRVFLETTGPKRGQNFRD
                     EGPFGHIVFFMVIFLISAFALLSYRGAGGFDIYTAILGLIVGASFILQYNLFRLIFPD
                     SDRLLLIVVDILAILGFVMLYRFSPDQGMHQIKYFGYGNIGMLFFVLFTRRIRMGKKL
                     GYTIMALACILLLLPIIFGKEIYGAKNWVSIMGFSFQPSEFVKIILVFVFAIWFNTGQ
                     RFRERLPALLFAGVSVMLVVLQKDLGAALLYFYIFLFVYYIATSDWIIAALALGTAAL
                     GSVISYRLFSHVRVRIEAWKNPWRDIENKGYQIAHSLIAISSGGLIGTGLGLGSPQVV
                     PAFKTDFIFATLCEEFGIITGLCVIGLYAVIVIRGMTIAIRSRESYWSLLALGATVAL
                     TMQAFTIIGGVIKMIPLTGVTMPFVSSGGSSMISSLALIGVLEGVAMGVPDVEDDDAD
                     EENHETYGEGDNEE"
     gene            7219..8679
                     /locus_tag="VFD89_06580"
     CDS             7219..8679
                     /locus_tag="VFD89_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZJ57891.1"
                     /translation="MKALRQNLKRGLVALMAAFVLLAAYYCYVLFFYGDRWFANSYNP
                     RVRMDAWEPKVIPGDIKDRKGKALASTIKENRKNPDTNEKQVFYYRSYSGGEQRAKSA
                     AHVVGFNSPQFGRSGVEALQVRYLLGYNNPFYERIYQKVFLSREVGNSLYLTIDADLQ
                     QYADDILGKNRGSVVVMNYQTGEVLAMVSHPTFNPNRLGEGIAGDVLVSRATEGLYAP
                     GSIFKIVVAASALENIDGALDDTFDCLGEIEIGTQKISCYGGEPHGQVDLARAMVLSC
                     NGDFARIGVELGKGKLLKTGEAFGFNQDFIFPDLKLVASRLPLKANVSKEKLALSSIG
                     QGDVQVTPLHMAMVVSAVANQGEMMEPKLVYQVVGRTGKEQKKMEPKVYRKPMGEEVS
                     RIIGDMMKDVVTHGTGKAAAIKGKQVAGKTGTAETAGQDSNPRNNAWFVGFVSDNSSK
                     VAIAVVLEDMPSGQTGGKAAAPLAGKILKKAVDLGY"
     gene            8727..>10462
                     /gene="uvrC"
                     /locus_tag="VFD89_06585"
     CDS             8727..>10462
                     /gene="uvrC"
                     /locus_tag="VFD89_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZJ57892.1"
                     /translation="MTEETTQKGWDFMMLLDEKLKNLPENPGVYLMRDETDNIIYVGK
                     AISLRNRVRQYFQSSKNHSPKVRAMVANIRDFEYIITDSELEALILECNLIKKHRPKY
                     NILLKDDKSYPYIKVTMEEDYPRIMLTRDVARDKNLYYGPYSSTKVVRDTIEVIKKLF
                     PIRTCKKNLNNMKKGDRPCLYYYINQCQGPCLGNITKEDYREVIRDVRKFLDGKYEDL
                     MVGLKEKMKEAAAELNFEKAASIRDKIISVEKVMETQKIVSVDMLDQDVIAIALGESE
                     SIVQMFFIRNGKLTGSEQFVLNTKNDNDIKEILGSFIKQFYLTSSFIPKEILLQQSID
                     EALIIERWLTSRKGNRVYIKTPQRGDKKKLVDMALKNAMEALENLKQKILREEARTIG
                     ASEELMQDLDLPYVPTRIEAFDISNIQGANSVASMVVFEKGRPSKQDYRRFRIKEIIG
                     PDDYASMSQVITRRFRRGLKEQGEMEAQGRDPNKGKFSRFPDLLLIDGGRGQLNVAAA
                     ALRELGIDSIPIISLAEKFDEIYTERMEEPLVIPKNSLGLQLLQRVRDEAHRFALTYH
                     RSLHSKTSIRSI"
BASE COUNT         2881 a         2126 c         2799 g         2656 t
ORIGIN      
        1 cgggcgaaag taagaagata catgtgtatc agaagagccg tatgcgggaa aaccgcacgt
       61 acggttctgt gaggggcaat agacattccc ttcacgatat aaatattttg aaaggagtgt
      121 cgagtttgtc tactcgacga atcgcaatgg gaaggattca aggagtatat ctagcccctc
      181 atccgccaat cattgtagat gaggtgggga agggggatag ggaaggcgct cggaccacca
      241 tagactccat gggtattttg ggcagagaaa tcaaaagcaa aaaccccgat acgattatcg
      301 tgataagccc ccatggaccg gtcttcgctg acgggatcgg cataatgtcg gaggatatat
      361 tgaaggggga tttttcgaga tttggtgccc caggtgtatc catggccttt caaacccatg
      421 gggatctcat agaaagaatc atcggcgagg cccaaaggat agggatttta gccatagatc
      481 tagataggga tatgtgctct tcatataata tatcgggtac gttggattgg ggcgttttag
      541 ttcccctcta cttcgtcaac caacacttta ccggatacag gttagtgact atgggcgttt
      601 cccagcttcc ctatcatgag ctgtacgcct tcgggatagc catgaagaga gcgatagaat
      661 cccaagacaa ggatgtcgtc ataatcgcca gcggggatct ctcccatcgc ttgaaggaag
      721 acggacccta cgggttccat cctgcagggg tggaattgga taagaccatt acgtctttga
      781 tagaagaggg cgatgtcgag gggcttttga ccatagatcc tgaacttata agtgaaggcg
      841 gggaatgtgg gctgcgatcc attatcatgg ctttaggtgc cttggacgga caagctttta
      901 ggccggaagt ccattcttac gaaggcccct ttggcgtggg ttattgtgtg gcatcctttg
      961 aacctgaaga cgccgacagt tcacgcaatc ttatggataa acttatagag aatagccgcc
     1021 gcagcataag ggatacgagg gaaaaagagg acgactatgt acggttggcc agggaatccc
     1081 ttgaaaccta tgtgaggacg ggggagacta tggatttagg cgacaggctc cccgattcta
     1141 tgctggacaa tcgggccggc acatttgtta ccattaaaca acacggtcag ctccggggat
     1201 gtatcggaac catagagccg tcacgggaga gcatagccca tgaaatcgtg gaaaatgcca
     1261 tcagcgccgg caccagggat cccaggtttt tccccgtggc ggaagaagag ctggatagtc
     1321 tggtgtattc cgtagatata ttgatggaac cggaaccggc agagggcttt ggggatctgg
     1381 acatcaaaaa atacggggta atagtcagcc acggctctag atccggtctc ctccttccaa
     1441 acctagaggg tattagttcc ccccaggagc aggtaagcat tgcccttcaa aaggccggaa
     1501 tagacccgga cgaggattat gagatgcaaa gatttgaggt gaagaggcat ctatgaagga
     1561 ggcattattc tacacgaggg ccgaaggggg aaagctaaga tgccttctat gccccaacgt
     1621 ctgtatcatc gaagaggggg acagggggag ctgcaacgtc agggtgaata tggagggcag
     1681 attgattgcc cagggatacg gtaatatatc cgccatagcc atggacccta tagaaaaaaa
     1741 gcccctctat catttctatc ccggctctaa catcctgtcc gtgggcggac tgtggtgcaa
     1801 cctaaggtgt tccttttgtc agaactggca cattgcccat cgtgagggcg agacccagtt
     1861 tatttctccc gaaagtttaa tcaccatggc caaagaatat ggcagcatcg gcatcgcatt
     1921 tacctataac gagcccatca taaactatga gtatattctt gacaccggta tcctggcggc
     1981 ggatcataat ctaaagatcg tgctggtcac caacgggtac atcctgccag aaccccttga
     2041 aaaattattg ccccatatag atgcgatgaa tatagatgtc aagggcttta ctgaggagta
     2101 ctacagggaa gtctgcgggg gaagcctctc gccgataaag agggccgtag aattggcggc
     2161 gggacattgc catgtagagc tgaccaccct tgtagtcgga ggggtcaatg acggggcgca
     2221 ggagatggag agcctcttta gatggataaa ggggatagac ccctatatac ccctgcacct
     2281 ttcccggtat ttaccgaact ataaaatgaa cttacctggc actgaggtgg agaccatgat
     2341 gtcgttaaag gcccttgggg ataaatattt gaatcatatc tatctgggga atatgggcgg
     2401 ggtggataat aacacctatt gtcctaattg ccgggaactt ttaatagaca ggggtcacta
     2461 tagggccagg gcagccggaa tatcccagga ggcaaggtgt agcggctgta gcagcgatac
     2521 aaggaggatt ttgggggcat ggggggacaa atagtaagat gattatcata ttttttacgc
     2581 ttgctataga aaaacatcat agaatgatgt tataattata ggggcggtct tatggggcct
     2641 atctaaaggt gtaattatgc aaacttcaaa aggtgtatta tactatggat taattcgtaa
     2701 taacgataat ctgattttat atggtttcta tttatagtta aacgataggt caaccatatt
     2761 tatctgtagg tgagaaattt catagttaga gttgtttata tgtagcggtt attctttcat
     2821 aatacctaaa gcgggggtgg aaatatgtgg ccgatgatat ctgatttatt gcagcgtttg
     2881 gaaccgtatt taaggtttcc ggatgttatt cgccatgtta tagatgttgc ggttgtagcc
     2941 ttcgtgttat accagctcct tttgctcatt agaaagacca gagcggaaca ggtgctgaaa
     3001 ggcttggtct tcctgttttt tatatacttt gtgactaagg gcttaaggct aaataccata
     3061 gcatggatcc tggaaaacac catcaatttt ggtattacgg cgggcgtaat tgtttttcat
     3121 cccgaaatta ggagggccct ggaacaaata ggcagggggc gtatttttga cagaaccctt
     3181 ctgatgcgcg acgaaaagga cagggatgca aggagcatcg tagacggcat cgtggcggct
     3241 gttgccaata tgtccaagac cagaataggg gcccttatag taatagaaaa gaataccgga
     3301 atcaatgaaa tcatcgaaac gggaatttgg attgacgcaa ggctgaccag ggagctgctg
     3361 gaaaacatat ttgtccccaa taccccactc catgacggag ccgttgtgat tcgggggaac
     3421 agagtggccg cagccggttg ttttctgccc ttgaccgaga acccaaatct gagcaaacaa
     3481 ttgggtaccc gccatcgagc ggccctggga atatcggaga gctcagatgc cttggcgata
     3541 attgtgtctg aggagacggg ggttatttcc atggccaaca gcggcaggct tactcgctat
     3601 ctggacatag acggcctcaa aaatgtcctg ggttcaatat atgttaagga ttccgatatt
     3661 gggcctttta gctttattaa aaggaggtcc aaaaatgggt agcttttttg aaagaaatac
     3721 aacccttaag ataatatcca tcataatcgc ccttatcctt tggggcatga ccccaaacaa
     3781 ccgggatccg gtgcgggata tgagctttag ggatattccc ataaagattg aaaaccagca
     3841 gaaactctcc gaaaacggac tgatgatatc gtcggagatg ccggacagct acagctttga
     3901 tatacgagcc aagggcagca tggtaaagtt tttggacagg agcaagatag ttgccctggt
     3961 taacttgtcg gatatcaaca agacgggtga acagagtgta tccgtcgagc tccaaggact
     4021 tccgggcacc agcaatattg agattaaatc agctccggac ataaaaattg acgtggagag
     4081 gatagtatcc aaatccatcc ctgtgttgcc caagatagca gacgaggaca gcctggaact
     4141 tggcaagcga ttctatgaaa tagagcccag atctgtagag gtcagaggcc ccgaatcact
     4201 catcgctacg gcggcatatg ggcaggtgtc cgtttcgttg ggggataagg ataaaaagtt
     4261 ggagcgctcg tctgcggttc agctgtttaa cgacgaggat gaggcccttg acatggggtt
     4321 tataaccata aaccccgaat actgcgtagt aaccatctac ccgaacaaga ccgtcgggat
     4381 agatgcggta attaccggta aaccggccga tggcttcgtc gtaatggggg aagaggtaaa
     4441 accccgggag gtaaccataa gcgggaatcc cgaggtattg gaggctatgg attccataca
     4501 gactgagata ttggatatcg aaggtgccac cagtaatatc gtaaaggaag tcaggctgca
     4561 acaacaggag gatataaggg tgagccccgg cgaaccatcc actgtgcaga tcctggtcag
     4621 gatagagaag atcgttgaca agcaggtctc cattgaggat gtagagtttc gcaatatagc
     4681 cgagggagtg gaagccaacg ttgaagagga cgggggagat atatccctta ccataagggg
     4741 cccacaaagc ctggtaaacg cctttggtcc cgaggatcta aggctgtatg tggacctcaa
     4801 cgaatcctca aggggcggga gaacctatcc catattggtg gataaattac cctcgggatt
     4861 ggagatagtg gatatagagc ctgagaatct aaaggtgata tttgagtgat tcaaaagaac
     4921 tttctcaccc cggtttcaat ctccgtcttt gcccggagca atacctggtt tatttcctcc
     4981 accttcacgg gctttaaaat ataatcgtag gccccgactt tcatggcctg ccgtaggtaa
     5041 tcaaagttat cgtagttgct gacgattacg tattttatat taggccagcg cttctttgcc
     5101 gtttcgatga aggtgatacc gtccatcttt ggcatcctga tctcggtgag tattacatgg
     5161 ggggctacat ggggcagaag gcccaatcct tcctcaccgt tatttgcctc gccgaccagt
     5221 tcaaaatcgt gttccaccca tttgaccttg ctatctatat ccctgcgtgc attatagctg
     5281 ttgtccacca ttaaaacctt taaaatcaac ttgttcatcc tctcttgcag caagtttgct
     5341 ggatttttta gtatagggtg ccaccaaatt aatggttggt aaattaaggt ttcaaaaagt
     5401 tgaattatag atattatgta ctaaggagtc ctataacatt tttttgaaac accccaatat
     5461 atttagattg attatagcat ctaaaacggg atataggaag aaagtttgtg ggaaccgcct
     5521 aaaaatgggg aaataattag aactggcatt tgacttaaat aaatgatata atcaagtata
     5581 tatttgcgtc tgtagctcag tggatagagt gttggactcc gactccaaag gccaagggtt
     5641 cgaatccctt cagacgtacc agtgaagaac ccctagatat ctaagtctct gggggttttg
     5701 tttttaatga ttccccatag gatcttgggt ttgtgtgcca ccttgtgtgc cgccgccctt
     5761 tgtttgtttt aatagatatt gggttaagat ggttgtatag gggctgaacc tgcagaaagg
     5821 ctgtgatatg gtgcctgata agaaattcga gataatagcc ttaaccatgc gctattggtt
     5881 tattggattg attatattcg ttgtcattag ggtgttttta gaaacgacgg gcccaaaaag
     5941 aggtcaaaat tttcgggacg agggtccatt cggacatatt gtttttttta tggtgatttt
     6001 tctcatctcc gccttcgcct tgctttccta taggggcgcc gggggatttg atatatatac
     6061 cgctatcttg ggattgatag tgggcgcgtc ctttatattg cagtataacc tctttcgatt
     6121 gatctttccg gattcggata ggctcttgtt gatagtagtc gatatattgg cgattttagg
     6181 tttcgtgatg ctctataggt ttagtcccga tcagggtatg caccagatca aatactttgg
     6241 ttacggaaat atcggaatgc tgttttttgt gctttttacc cgacggatca ggatgggtaa
     6301 aaaattgggc tacacaatta tggcgcttgc ctgtatattg ttgttattgc cgattatatt
     6361 tggaaaggaa atctatgggg ccaagaattg ggtatccata atgggtttta gctttcaacc
     6421 gtcggagttt gtgaagataa tcctggtatt tgtttttgcc atatggttta atacgggcca
     6481 aaggtttcgg gagcggttgc ctgccctttt gtttgcaggg gtttctgtta tgctggtggt
     6541 acttcagaag gatttaggtg cagccctgtt atacttttac atcttccttt ttgtctatta
     6601 tatagctacc tccgattgga taatcgccgc tttggccttg gggacagccg ccttggggag
     6661 cgtaataagt tatcgcttat ttagccatgt gcgggtacgt atagaggcgt ggaagaaccc
     6721 ttggagggat atagagaata agggatacca gatagcccat tcccttatag ccataagcag
     6781 cggcggtctt atagggacgg gcctgggttt aggctcgccg caagtggttc ccgcgtttaa
     6841 gacggacttt atattcgcga ccctatgcga ggagtttggg atcattacgg ggctctgcgt
     6901 catcgggctc tacgccgtca tagttattag ggggatgacc atcgccataa ggagccggga
     6961 atcatattgg tcactgctgg ctttgggggc aacggtggcc ttaaccatgc aggcttttac
     7021 catcataggg ggcgttatca agatgatacc cctaacgggt gttaccatgc ctttcgtcag
     7081 ttcagggggc agctcgatga tatcttcgct ggctctcata ggggttttag agggtgtggc
     7141 gatgggcgta cccgacgttg aggacgatga tgcagatgaa gaaaaccatg aaacctatgg
     7201 ggaaggggat aatgaggaat gaaggcttta aggcagaacc taaagagagg tctcgttgcc
     7261 cttatggctg cctttgtctt gttggctgcc tattattgct atgttctctt cttctacggg
     7321 gacagatggt tcgcaaattc atataatccg agggtgcgta tggatgcatg ggagcccaaa
     7381 gtcatccccg gggatataaa ggatcgaaag gggaaggccc tggcttcgac cattaaggag
     7441 aatcgcaaga acccggacac aaacgaaaaa caggtctttt actataggag ttattccggc
     7501 ggcgaacaac gggctaagag tgcagcccat gtggtggggt ttaacagccc ccagtttggc
     7561 agatccgggg tagaggcatt acaggtccga tatctcttgg ggtacaacaa tcccttttac
     7621 gagaggatat atcaaaaagt atttttatca cgggaggtgg gaaacagcct atacctcaca
     7681 atcgatgccg acctccagca atatgccgat gatatcctcg gaaagaacag gggcagcgtc
     7741 gtagtcatga actatcaaac gggggaggta ttggccatgg tcagccatcc gaccttcaat
     7801 cccaaccgct taggggaggg tattgccggc gatgtcctgg taagcagggc gacggaaggg
     7861 ctttatgccc ccggctcaat ctttaagata gtagttgccg catctgccct ggagaatata
     7921 gacggtgccc tcgacgatac ctttgattgt ctgggagaaa tagaaatcgg tacacagaag
     7981 atctcatgct acgggggtga accccacggt caggttgacc tggcccgggc aatggtgctg
     8041 tcttgtaatg gcgatttcgc ccgaataggt gtagaattgg ggaagggaaa gctccttaaa
     8101 accggcgagg cctttggctt taaccaggat ttcatatttc ctgatttaaa gctggttgcc
     8161 agccgtttac ccctcaaggc caacgtttcc aaggagaaat tggccctatc ctccataggc
     8221 caaggggatg tgcaggtaac tcccctgcat atggccatgg ttgtgtctgc ggtggccaac
     8281 cagggcgaaa tgatggaacc caaactcgtc taccaggtgg tcggaaggac cggcaaggaa
     8341 caaaaaaaaa tggagcctaa ggtctatcga aagcctatgg gcgaggaagt atcccgaatc
     8401 ataggggata tgatgaagga tgtagttacg catggcacgg gaaaagccgc tgctatcaaa
     8461 gggaaacagg tagcgggtaa aaccgggact gccgaaacgg caggccagga ttccaatccg
     8521 aggaacaatg cttggtttgt tggatttgtt tccgataaca gctccaaggt agccatcgcc
     8581 gtagtcctcg aggatatgcc ctcgggtcag accgggggaa aggctgcagc gccgctggcc
     8641 ggaaagatac ttaaaaaggc cgttgatctg ggttattgaa tgaaatttat caacgcggaa
     8701 tgatccaagc tattagtgga ttgtttttga ctgaagagac tacacagaag ggttgggatt
     8761 ttatgatgct attggatgaa aaattaaaaa acctgcctga aaaccccggc gtttacctga
     8821 tgagggatga gacagataat ataatctatg taggtaaggc catttccctt agaaatcgtg
     8881 tccgccaata ttttcaatca tcgaaaaatc actcgcctaa ggtgagggcc atggttgcta
     8941 atatcaggga ctttgaatat ataatcacgg attcggagct ggaagccctg atcctggaat
     9001 gtaacctaat aaaaaaacac aggccaaaat ataacattct attaaaggat gataagagtt
     9061 acccgtatat caaggttacg atggaagagg attatccgag gattatgctg acccgggacg
     9121 tagccaggga taaaaacctc tattacgggc catacagcag caccaaggtg gtcagagata
     9181 ccatagaggt cataaaaaaa ctgtttccca taaggacgtg taagaagaac ctaaataaca
     9241 tgaaaaaagg ggatcgaccc tgcctgtatt actatataaa tcaatgtcag ggcccttgcc
     9301 ttggaaatat caccaaggag gattataggg aagtcataag ggatgtgcgt aaatttctag
     9361 acggtaaata tgaggacctt atggtggggt taaaggagaa gatgaaggaa gctgccgcag
     9421 agctgaactt tgagaaggcc gcctctatca gggataaaat catttcagtt gaaaaggtaa
     9481 tggaaacaca gaaaattgta tccgttgata tgttggatca ggatgttata gccatcgccc
     9541 ttggcgaatc tgaaagtata gtacagatgt tttttataag aaatggcaag ctgacgggga
     9601 gcgagcagtt cgtattgaat accaaaaatg acaacgatat aaaagagata ttgggctctt
     9661 ttattaagca gttttatctg acatcgtcct ttatccccaa ggaaatcctt ttacagcaaa
     9721 gcattgacga ggctttaatc atcgaacgct ggcttacttc tagaaagggg aatcgggtat
     9781 atataaaaac gccccagaga ggagataaga aaaagctggt ggatatggcc ctaaagaacg
     9841 ccatggaggc attggaaaac ctaaagcaga agattttgcg ggaggaggca aggaccatag
     9901 gtgcctccga ggagctgatg caggatctgg accttcccta tgtgccgacc aggatagagg
     9961 cttttgatat atccaatatc cagggagcta attctgtggc ttccatggtt gtatttgaaa
    10021 agggtaggcc cagcaagcag gattacagga ggtttaggat aaaagaaatt attggacccg
    10081 acgactatgc cagcatgtcc caagtgatta ccagaaggtt tagaagaggg ctgaaagagc
    10141 agggagaaat ggaagcccaa ggcagagatc ccaataaggg taaattttca aggtttccgg
    10201 atcttttatt gatagatggt gggagaggcc agctaaacgt cgcagctgcc gcattgaggg
    10261 agcttgggat tgattctatt cccataatta gcctggcaga aaaattcgat gaaatctaca
    10321 cagagcggat ggaagagccc ttggtgatcc ccaaaaacag tcttggactc cagctgctcc
    10381 agagggtgag ggacgaagca cacaggttcg ctcttaccta tcacaggagc ctccatagca
    10441 agaccagcat caggtccata tt
//
LOCUS       DASRNX010000165         4147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_564244,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000165 DASRNX010000000
VERSION     DASRNX010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564244"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..714
                     /locus_tag="VFD89_06590"
     CDS             70..714
                     /locus_tag="VFD89_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57893.1"
                     /translation="MILIDDAGSGSLVGGTCIAALRSETNEYVYDFIPISYYQNGNFY
                     EKLYLHKSKDIVFELIAKLGITKKDEILICRGYMFDIARKFLSDSQLNWTSTKIVDPL
                     QSKIEHTFNQYALGLGVPVQYVNYIKYPLHFHRILRWVYADYPNRFNLCKTDWKSFKK
                     YGNLNVSISQDILYKKNYTCLKCGRKINPGTKVKVLKYISNCPNTIYLHHNCSP"
     gene            complement(792..2189)
                     /locus_tag="VFD89_06595"
     CDS             complement(792..2189)
                     /locus_tag="VFD89_06595"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HZJ57894.1"
                     /translation="MAKVKITDTILRDAHQSQAATRMRLDEMLPIADKLDRVGYHSLE
                     VWGGATFDSCLRFLKEDPWERLRTIRKAIPNTKLQMLLRGQNLLGYKHYADDVVDEFV
                     KKSIDNGIDIIRIFDALNDVRNMEQAIKSTKKYKGHVQAALCYTTSPVHTIDYFVDLA
                     KRLENLGADTICIKDMANLLLPYKAYELVTKLKNTLKVPIQLHTHNTTGTGDMVYLKA
                     IEAGVDVVDTALSPLGNGTSQPATEPLVATLQETQWDTGLDLNLLNEIADHFKVVANR
                     LLEDGFLNPKVLSIDINTLIYQVPGGMLSNLLSQLKQQNAEDKFEEVLKEVPRVREDF
                     GFPPLVTPTSQIVGTQAVLNILTGERYKMITNESKGLIRGEYGQLPGEVSDDIRKLII
                     GDAAVITHRPADDIPPELDKYRDEIKEYLEQEEDVLSYASFPQVSMEYFKYRQAQKYN
                     IDNTLINKEEMTHPI"
     gene            complement(2213..3373)
                     /locus_tag="VFD89_06600"
     CDS             complement(2213..3373)
                     /locus_tag="VFD89_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014041810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HZJ57895.1"
                     /translation="MDKPLMEVITGFLADTGFAQLSWQQGIMLLVSFVLIYLAIKKEY
                     EPLLLLPIAFGMLLANLPLAGLNDAPVGIYGEDNFQPGGLLYYLYMGVKLGIYPPLIF
                     MGVGAMTDFAPLIANPRSLLLGAAAQLGIFVAFLLAILLGFPGNVAASIGIIGGADGP
                     TAIFVTTKLAPEYLGAIAIAAYSYMALVPIIQPPIMKALTTKKERQIVMEQLRPVSKL
                     EKIIFPIIVTVLGSLLLPSAAPLLGMLMLGNLFRESMVVDRLTKTAQNELINIITIFL
                     GVTVGATAKAENFLTPDTLKIIALGLLAFSFGTAGGVLLGKVMCKVSGGKINPLIGSA
                     GVSAVPMAARVSQVVGQQENPGNFLLMHAMGPNVAGVIGSAISAGVFLNLFG"
     gene            complement(3407..3808)
                     /locus_tag="VFD89_06605"
     CDS             complement(3407..3808)
                     /locus_tag="VFD89_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZJ57896.1"
                     /translation="MRKFVINVNGKSYEVEVEEVGQGTTKPMPVSAAPVSNPIATPAP
                     APKRTETPKPASGGAVPQGANTIKAPMPGTILDIKVQTGDRVSKGQVLAILEAMKMEN
                     EIMSPGDGEIVSVQVAKGASVNAGDVLIAIQ"
     gene            complement(3844..>4147)
                     /locus_tag="VFD89_06610"
     CDS             complement(3844..>4147)
                     /locus_tag="VFD89_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57897.1"
                     /translation="YVIELINKILNLNEGQSNKENKHIVMDSSEFGNGEESPDAIDDP
                     NQEHDDEEIVAVIAAAVAASLSYSTHDIIVRSIRRISSNTPAWNRAGRSQQIAGKL"
BASE COUNT         1150 a          958 c          834 g         1205 t
ORIGIN      
        1 gccacttttc atacaataga gtaaatcctg ttagatttaa aaggttgcat aatctaaagg
       61 ggtgaatctg tgatactaat agacgatgcg ggcagcggta gtcttgtggg tggtacatgt
      121 attgccgcac ttcgttcgga aacaaatgaa tatgtctatg attttatacc aatcagctat
      181 taccagaatg gaaatttcta tgaaaagctt tatttgcaca agagcaagga tatagtcttc
      241 gagctcatag caaaactcgg gataactaaa aaagatgaaa ttttgatttg tcggggctat
      301 atgttcgata tagccagaaa gttcctgagc gattctcaat taaattggac aagcaccaag
      361 atcgttgatc ccttgcagag caaaatagag catacattta atcagtatgc cctggggctt
      421 ggcgtgccgg tgcaatatgt taattatata aaatatccct tgcactttca tcgaatatta
      481 agatgggtct atgcagatta tcccaatagg tttaatcttt gtaaaacgga ttggaagagc
      541 tttaaaaaat atggcaacct aaatgtttct atttcccagg atatactgta taagaaaaat
      601 tatacatgcc tcaagtgcgg cagaaaaata aacccgggta ctaaagtgaa ggtcttaaaa
      661 tatattagta actgccccaa taccatatac ctccaccata actgttctcc ctaaccgctt
      721 ttcggtccat aaaacaaaca aatccaaagg taaaagtccg gggatccact gggttccccg
      781 gacttaaaca attaaatcgg atgtgtcatc tcttccttat tgataagggt attatctata
      841 ttgtattttt gcgcctggcg atatttgaag tattccatag atacctgggg gaaggatgca
      901 tatgataaaa catcctcttc ctgttctaaa tattccttta tttcgtcccg atatttatca
      961 agttcgggcg gaatatcatc cgcaggccgg tgggttataa cagcggcatc gcctataata
     1021 agtttccgaa tatcgtcact gacctcaccc ggcagctgcc catattcccc gcgtattaac
     1081 cctttagatt cgttggtgat catcttgtat ctctcacctg tcaagatatt tagtaccgcc
     1141 tgggtgccta ctatttggct cgtgggggta accaacggcg ggaacccaaa atcttcccta
     1201 acccttggca cttcctttaa tacctcctcg aatttatctt cagcattctg ttgctttagc
     1261 tgggataata aattggacag catcccgccg ggaacctgat aaatcaaggt gtttatatct
     1321 atactcaaaa ccttcgggtt taagaaccca tcctctaaga gcctgtttgc cacaaccttg
     1381 aaatgatctg ctatctcgtt cagcaaattc aaatccagac cggtatccca ctgtgtttcc
     1441 tggagggtag ccaccaaagg ttcagttgcc ggctgggaag tcccgtttcc taagggagag
     1501 agtgcggtgt ccactacatc cactccggcc tctattgcct ttaaatagac catatcaccg
     1561 gtaccggtgg tgttatgggt atgaagctgg atgggaacct taagggtatt tttcagtttc
     1621 gtgaccagct cataggcttt atagggcaac agcagatttg ccatatcttt gatgcatatg
     1681 gtatctgccc ccaagttttc caaccttttt gcaagatcca caaaataatc tatggtgtgt
     1741 acagggctgg tagtatagca cagcgcggcc tgaacatggc ccttatattt ctttgtggac
     1801 ttaatagcct gctccatatt tcggacatca ttgagggcat caaaaattcg aatgatatcg
     1861 ataccgttat ctatggattt tttaacaaat tcgtctacaa catcatctgc atagtgttta
     1921 taaccaagca aattttgccc tctaagcagc atttgcagct tggtatttgg gatggctttt
     1981 cttatggttc taagtctttc ccagggatcc tcctttaaaa agcgaaggca tgaatcaaac
     2041 gtagcaccgc cccacacttc cagggaatga tatcccactc tgtctaattt atctgcaata
     2101 gggagcatct catccagtcg cattctagtt gctgcctgag attgatgggc atcccttaaa
     2161 atggtatcag ttattttaac ttttgccatg accattacct cctatataat gattagccga
     2221 aaaggtttag aaaaaccccg gctgatatag cggacccgat tactcccgcg acattgggac
     2281 ccatagcgtg catgagtaaa aagttgcccg gattttcctg ttggcctact acctgagaca
     2341 cacgcgctgc cataggcacc gcggagacac cggcagaacc tatcagggga ttgatcttgc
     2401 cacctgaaac cttgcacata acttttccta ataatactcc ccctgcggtt ccaaaagaaa
     2461 aggctaaaag tccgagggca attattttca gggtgtccgg agtaagaaag ttttcggcct
     2521 ttgccgtagc ccctacggtt acccccagaa agatagttat tatgttaatc aattcatttt
     2581 gtgcggtttt ggttaaccga tctaccacca tactctccct aaagagatta cccagcatta
     2641 gcattcccag taacggggct gcagatggga gtaataagga ccccaaaaca gttacaataa
     2701 tgggaaaaat aatcttttcc aatttagata cgggacggag ttgctccatc actatttgtc
     2761 tttctttttt tgtggtcaag gccttcatga tgggcggttg aataattgga accaacgcca
     2821 tatacgaata ggcagcaatt gcaatggctc ccagatattc cggcgccagc tttgtggtta
     2881 cgaatatagc cgtaggtccg tcagcaccgc caattatgcc gatggaagca gcaacattac
     2941 ccggaaatcc caataagata gccagtaaaa aggctacaaa tatacccaat tgggcggctg
     3001 cacccaaaag aagacttctg ggatttgcta taaggggcgc aaaatctgtc attgcaccaa
     3061 cacccataaa aatcaaaggc ggataaatgc ctaatttaac tcccatgtac agataatata
     3121 acagtccgcc gggctggaag ttgtcttcac catatatgcc cacaggagca tcgttcagac
     3181 ccgccaaagg taggtttgcc agtagcatac caaaggctat gggaagaagg agcagcggtt
     3241 catattcctt tttaatagca aggtatatga gtacaaacga aaccaaaagc attattcctt
     3301 gctgccatga cagctgtgcg aaacctgtgt ccgccaaaaa cccggttatt acctccatta
     3361 atggtttatc cataaaatat ccctcctcgt tgctatttgg tgttttctat tggatggcaa
     3421 tcagaacatc cccagcattt acagatgcac ctttagcaac ttgaacagaa acaatttcac
     3481 cgtctccagg actcataatt tcattttcca ttttcatggc ctccaaaatt gccaggactt
     3541 gaccctttga aaccctatcg ccggtttgta ccttgatgtc taaaattgta cccggcatag
     3601 gtgcctttat ggtattagcc ccttgtggaa ctgcaccccc tgaggctggt ttgggggttt
     3661 ccgttctttt tggagcggga gcaggtgttg ctattggatt tgatacagga gcagcactaa
     3721 caggcatcgg tttggttgtc ccctgaccta cttcctcaac ttccacctca taggatttcc
     3781 cattgacatt tataacaaac tttctcataa tttttcctcc tcaatataga aacctgtact
     3841 ttcttatagt ttccccgcga tctgttgact acgacccgcc ctattccacg caggggtatt
     3901 agaagatatc ctcctaatgg acctaacaat aatatcatgg gttgaatagc ttagactggc
     3961 ggcgacagca gccgcgatca cggcaactat ctcctcatcg tcatgttcct gattgggatc
     4021 gtcaattgca tcagggcttt cttccccatt cccaaattcg ctagagtcca ttacaatgtg
     4081 cttattttct ttattgcttt gaccttcatt caggtttaat atcttgttaa ttaattcaat
     4141 aacatat
//
LOCUS       DASRNX010000166         5910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_720797,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000166 DASRNX010000000
VERSION     DASRNX010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720797"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFD89_06615"
     CDS             <1..185
                     /locus_tag="VFD89_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57898.1"
                     /translation="RKIAYVDDQDIPQIDVASFENIRGNAVRSVFFEVYKNGGYGILF
                     RNHTLGKWEETLLINP"
     gene            379..1923
                     /locus_tag="VFD89_06620"
     CDS             379..1923
                     /locus_tag="VFD89_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZJ57899.1"
                     /translation="MNIVFAFGDDWGRYASAYRQHDKPNSVNQLITTPNFDRVADEGV
                     LFTNAFVPAPSCTPCRSSVLSGKYFWQTGLGAILIGAVWDDEIPSFPLELEKKGYHIG
                     HTYKVWSPGKSYNAPYGGKRTSYEAAGTDFNQFSHHVTERANIVGIEAAKQELLDEVK
                     MNFNDFLSSRPEDKPFCYWWGPTNTHRTWEQGSGKNLWGLEPDDLKARMPEFLPDVPE
                     VREDFCDYLGECLAFDAGLGVILKTLEEKGELDNTLVVVSGDHGIPGMPRAKCNLYDI
                     GCRVALGMRWPKHIDPGRVVDDYINLMDLAPTFMDAAGAAIPQSMVGRSLMPILKSKK
                     SGHIEQERDFVVLGRERHVHSARQGYLPYPQRAIRTEDYLYIINFAPDRWPMGDPMGL
                     DNPTATPPGYEDLKWDTFVAYADLDASPTKAWMVYNRGEDDVKPLFDLGFHKRPREEL
                     YDLRKDKDYMINEASNPEYSAVKNRLHHRLMEVLEKYDDPRVVETPCPFEMAPFAGEL
                     QEFQRE"
     gene            1957..2085
                     /locus_tag="VFD89_06625"
     CDS             1957..2085
                     /locus_tag="VFD89_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57900.1"
                     /translation="MKMGAATPSPKMEWISHETEDLRLIPYGSTNLRMTEIPTLDD"
     gene            2097..3785
                     /locus_tag="VFD89_06630"
     CDS             2097..3785
                     /locus_tag="VFD89_06630"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZJ57901.1"
                     /translation="MKLKIRAFDQNKKAIPCKITIQKLSFDDSQYAEGSVNTVYCSGT
                     YECSLENGLYDINVYRGKLFKPYRERIHMGGGDIYIDAVLHELLDIEAMGLYAFDGHS
                     HVSRDRELVSGNLQKAALVMKGEGFHFFFAGSPYDNETHLECLKDSYTEGKSYRKHFA
                     STLDRLNDDEFFIDIGNEIIKCRYGHIFLMNYTQYSPFNKYYDHEFDPWLFNKEGEEP
                     PYRIPYMYEALALEKEGHSVAVFAHPTSWWWHDNGEFITNIATTLGFEILAESIDAMV
                     VMGYKSDQIHYQQLWFDALNNGYFLPGIAETDTLLDTVPSKFTSFKTYTYFDGKFGNI
                     DALCDAIKKGRNIVSTGPLVTLKVEGELPGSVLSYSDDDRFHIDVEAFACFEGFLSKV
                     QLIINGEVYKEYNIRQNRFRAEECMGFQGDSFIIAKCYDYAGNVSFTNPVYIRNSPFV
                     NRGYLSHVKVTVLKDNGPASGVYSIEGGSEHPFENEIDIKMGVSSGLRITVGDEIRTI
                     RLFELEELQDIFKNLYFGRFNAHNRYLPGEVPADCFRLRRIRQILDEVELTLKF"
     gene            3801..4832
                     /locus_tag="VFD89_06635"
     CDS             3801..4832
                     /locus_tag="VFD89_06635"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZJ57902.1"
                     /translation="MTAVNRGLFLDAEHRLKLREFPIPKPAGGELLVEIRANGICGSD
                     VQFFKEGRLGNFEVTVPYIPGHEASGVVVGTGPDVKHFKEGDKVVVEPGFPCGRCPFC
                     KTGRYNLCPDVIFLSAPPINGTFCDYITMPESFAHPIPEGMSFEKAAVAEPAAVAVHA
                     VNCAKYANGAHGVIIGTGPIGLLTLQAFKAAGGGQVTCVDLDAKRLQAAKKLGADRVI
                     NLKDEDMPMDIGDVVFETAGSTEATAGLFSVARVGGTVVQVGWPGDNIVNMNIASFIE
                     KELRYLGVNRYANAFPTAIQWLADGRISTDGIITHRFPLSRGPEAFRIASDNTQGSIK
                     VIVYNSEDN"
     gene            5226..>5910
                     /locus_tag="VFD89_06640"
     CDS             5226..>5910
                     /locus_tag="VFD89_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZJ57903.1"
                     /translation="MIKRYLGLLLVIVLFISLTSCSGGKTKGKTDQYGNDEDDIIGIT
                     IKGETLEVVITYPREKAIVFEEVVADFEDITGAIAEITAYHNDYENKLQARMAANSLP
                     DIFHAHGWSILDYREYFKEYFADLQNESWVGLYTDSALEVTRDDDGSVYVLMISQQAN
                     ATLVNLDVCAKAGVDPYLIYDWDDFTDACQRIMDAGFTPIAAAAGNPGTLTNIAGSWL
                     NYKGVPVDET"
BASE COUNT         1634 a         1235 c         1560 g         1481 t
ORIGIN      
        1 ttcgaaagat tgcatatgtg gacgatcagg acattccaca gatcgacgta gcatcctttg
       61 agaatattcg tggcaacgca gtccgctcgg tcttctttga ggtctataaa aacggtgggt
      121 atggaatact gtttcgcaac catacccttg ggaagtggga ggaaacgctt ttaattaacc
      181 cgtaacgaat acgagagagc tgccagactg tagcatgtgt ggggggaatc gctaccatgg
      241 gcgtagcctt gatgtaacgt gaggatatgg gaggaatcgt caggctgttt tgcatggtgg
      301 aaccgcccct aggggcggac cagcgttttc aaagaacaca taggaaccgt ccccctgtgt
      361 taaaaggagg agatttcgat gaatatagtt tttgcatttg gcgatgattg gggcaggtat
      421 gccagcgcct atagacagca tgataagcca aattcagtaa atcaattgat aaccacgcct
      481 aactttgatc gggtagcgga tgaaggcgtt ctatttacca atgcttttgt gcccgcgcca
      541 agctgtaccc cctgccgtag ttcagtcctt tccggaaagt atttttggca gacaggactt
      601 ggagcgatac tcattggcgc cgtatgggat gacgaaatcc catcgtttcc cttagaactc
      661 gaaaagaaag gctatcacat cggccataca tacaaggtat ggtctcccgg aaaatcctat
      721 aacgctccat atggcggaaa acggacatcc tatgaagccg cgggtaccga cttcaaccaa
      781 ttttcccatc atgtaacgga gcgcgccaat atagtcggaa tcgaggccgc caagcaggag
      841 ctattggacg aagtgaagat gaactttaat gattttctta gttctcgtcc ggaggataag
      901 cctttttgtt attggtgggg accgacaaac acccaccgaa cctgggaaca agggtcgggg
      961 aaaaacctat ggggcttaga accggatgac ttaaaggcca ggatgcctga gttcttaccc
     1021 gatgttccgg aagtgcgtga agacttttgt gattatctgg gcgaatgcct ggcttttgat
     1081 gcgggacttg gggtcatctt aaagaccttg gaagaaaagg gggaacttga taatactttg
     1141 gttgttgtca gcggagatca tggcatcccc gggatgcccc gtgccaagtg caacctatac
     1201 gatatcggat gtagggtggc tttgggaatg cggtggccaa agcatataga ccccggccgc
     1261 gtggtagacg attatataaa ccttatggac ttggccccta cctttatgga tgcagcggga
     1321 gccgcaattc cccaatccat ggttggaagg agtctaatgc ccattttaaa gtccaaaaag
     1381 agcggtcata tagagcagga acgggacttt gtggttctgg ggcgagaacg ccatgtccat
     1441 agcgctcgcc agggttatct cccatatcct caacgggcga tccgaacgga agattaccta
     1501 tatattatca attttgcccc tgataggtgg cccatgggtg atcctatggg actggacaat
     1561 ccgactgcca caccgcccgg ctatgaggat ctaaaatggg atacatttgt tgcctacgcg
     1621 gatttggatg caagccccac caaggcttgg atggtctata atcgaggcga agatgatgta
     1681 aagccgctct ttgatttagg atttcataaa cggccccggg aagagctcta tgatctacga
     1741 aaagataagg attacatgat aaacgaggcg tcaaacccgg aatatagtgc ggttaaaaac
     1801 aggctgcacc ataggttaat ggaggtccta gaaaaatacg atgatccacg cgtggtggag
     1861 acaccctgtc cctttgaaat ggctccattt gccggcgaac tccaagaatt tcaaagggaa
     1921 tgagccgggt ataagggaga gccaagggac tgggggatga aaatgggggc ggcaacccca
     1981 tcgcctaaaa tggaatggat ttcccacgag acagaggatc tcaggctgat cccgtatgga
     2041 tccacaaatc tgcgcatgac ggaaattcct acattggacg attgaggggt ggcattatga
     2101 aactgaaaat cagggcattt gatcagaaca aaaaagcaat accatgcaag attacgatac
     2161 aaaagttgtc atttgatgat tctcaatatg cggaaggatc agttaatacg gtttattgta
     2221 gcggcacata tgaatgcagc ctagaaaatg ggctctatga tatcaatgta tataggggta
     2281 agctatttaa accctacaga gagcgtatcc acatgggcgg tggcgacata tatatagatg
     2341 ctgttctgca tgagctgctg gacatagagg ccatggggct gtacgccttt gacgggcatt
     2401 cccatgtatc cagggacagg gaattggttt ccgggaacct ccaaaaggcg gcccttgtca
     2461 tgaaggggga aggttttcat ttcttctttg ccggttcgcc ctatgacaac gagacccatc
     2521 tggaatgttt aaaggatagt tatactgaag gcaaaagcta taggaaacac tttgcatcca
     2581 cattagatcg actcaacgac gatgaatttt ttatcgatat cggaaacgaa atcataaagt
     2641 gtcgctatgg gcatatcttt ttaatgaact acacacaata ttcgccgttc aacaagtatt
     2701 acgaccatga atttgatccg tggcttttta acaaggaggg ggaagaacct ccatatagga
     2761 ttccctatat gtacgaggca ctggcacttg aaaaggaggg acattccgtt gccgtatttg
     2821 cacaccctac aagctggtgg tggcatgata acggtgaatt cataaccaat atcgccacga
     2881 ccctgggctt tgaaatactg gcagaatcca tcgatgccat ggtggttatg ggctataaaa
     2941 gtgaccagat tcattaccag cagctttggt ttgatgcgtt aaataacggg tatttcctgc
     3001 cgggaattgc ggaaaccgat acactcttgg acacggtccc cagtaaattc acttcattta
     3061 agacatatac gtactttgac ggtaaattcg ggaacattga cgccttgtgc gatgcgataa
     3121 agaagggacg caatattgtt tccaccggtc ctttggttac ccttaaggta gaaggcgaat
     3181 tgccgggatc cgttttaagt tattccgatg atgaccggtt ccatatcgat gtggaggcct
     3241 tcgcctgctt tgagggtttt ttgagcaagg tacagctcat cataaacggg gaagtataca
     3301 aagaatataa tatccgacag aaccgtttta gagcagaaga atgcatgggc tttcaaggag
     3361 acagctttat tatagccaaa tgctatgatt atgcgggaaa tgtttcattt accaatccgg
     3421 tgtatataag aaacagccca tttgtaaata ggggatattt atcccatgtg aaagtaaccg
     3481 ttttaaaaga taacggtccc gcctccggtg tgtatagtat tgaggggggg tcagaacatc
     3541 catttgaaaa tgaaattgac atcaagatgg gggtatcgtc cggattaagg attacagtcg
     3601 gtgacgagat aaggacgata cggctgtttg aattggagga actgcaggat attttcaaga
     3661 acttgtactt tggtaggttt aatgcccata acagatatct gcccggggag gtccccgcgg
     3721 attgttttag acttaggcgt ataagacaaa tcctggatga ggtggaattg acattgaaat
     3781 tttaagggga gggagcaaaa atgacggctg taaacagagg tcttttcttg gatgcggagc
     3841 atagactcaa gctgagggaa tttcccatac ccaagccggc cggtggggaa ctccttgttg
     3901 aaattcgtgc caacggcata tgcggatcgg atgtccagtt ttttaaagag ggcaggctgg
     3961 ggaattttga ggtgacggtc ccctatattc ccggacatga ggccagcggg gttgtggtcg
     4021 gaactgggcc ggatgtaaag catttcaagg aaggggacaa ggtggttgtg gaaccgggat
     4081 ttccatgcgg aaggtgtcct ttctgcaaga ccggcagata taatctgtgt cccgatgtga
     4141 tattcctttc cgcaccgccc ataaacggta cgttttgtga ttatataacg atgccggaaa
     4201 gctttgcgca ccctattccg gagggcatgt ccttcgaaaa ggctgcggtt gccgagcccg
     4261 ccgcagttgc tgtacatgca gtcaactgcg caaagtatgc aaacggcgcc catggggtta
     4321 ttataggaac cgggcctata gggctcttaa ccctgcaggc ctttaaagcc gccgggggcg
     4381 ggcaggtcac gtgtgtggat ctggatgcga agcggcttca ggcagcgaaa aagctcggag
     4441 ccgacagagt tataaaccta aaagatgagg atatgccaat ggacatcggc gatgtcgtat
     4501 tcgagaccgc aggatctacc gaagccacgg cagggctatt ttcagtcgcc agggtgggcg
     4561 gaaccgtagt tcaggtgggc tggccgggag ataacatagt caatatgaac attgcatcct
     4621 ttattgaaaa ggaactccgg tatctggggg tcaaccggta tgccaatgca ttcccaacgg
     4681 cgatccaatg gcttgccgac ggcagaatct ctacagacgg aatcatcacc catcgctttc
     4741 ccctaagtcg cggccctgag gcctttcgta tcgcctccga taatacccag gggtcaataa
     4801 aagtaattgt gtataacagc gaggataatt aattatatca caagggacta ttccgttaag
     4861 ctggaggcca atatacatgg tcggttattg aattatcaaa ttggcacaag gtttaggata
     4921 ccggagtacc ggcgtacatt agataacgat gaacaatacg gaaactaaat ttattatcat
     4981 aataattgca gtggattcat gcaatgtcat gataatcaat tttctatatg agatcttttt
     5041 ctataacgag tcggcaagat gtctcccgct tctataagcg gtgagttccg attatagtaa
     5101 aacaggcggt gggtatgaat ttcccgcctc gttgaaacct gtgagttgca attgccaatg
     5161 tcttgcttcc gatgttataa caacgcgtat acggctgaaa tttaagatta atggggggtg
     5221 aggggatgat aaaaagatat ctgggtctac tactcgtaat agtcttgttc atatcgctta
     5281 cttcgtgcag cggcggtaaa acgaagggta agaccgatca atacggcaac gatgaggacg
     5341 atattatagg gattacaatt aagggcgaaa cactggaggt tgtcataacc tatcccaggg
     5401 aaaaggccat agtatttgaa gaggtggttg ctgattttga ggacattaca ggggcaatcg
     5461 ctgaaattac cgcataccac aacgattatg aaaacaaact tcaagcccgt atggccgcga
     5521 attccctgcc ggatattttc catgcacatg ggtggtctat tttagattac agggaatatt
     5581 ttaaagaata ttttgccgac ctccaaaatg aatcctgggt gggcctgtat accgattctg
     5641 ccctggaggt aacaagggac gacgacggat cagtttatgt gcttatgatt tcccagcagg
     5701 caaacgctac actggtcaat cttgatgtat gcgcaaaggc cggcgtcgat ccttatttaa
     5761 tttatgattg ggacgacttc acagacgctt gccaaaggat tatggatgcg gggtttacac
     5821 caattgctgc cgctgccggc aaccctggaa cccttacaaa tatagcaggt agctggctta
     5881 actacaaggg agttccggta gacgaaacgg
//
LOCUS       DASRNX010000167         8785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_671627,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000167 DASRNX010000000
VERSION     DASRNX010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671627"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..2120)
                     /locus_tag="VFD89_06645"
     CDS             complement(321..2120)
                     /locus_tag="VFD89_06645"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZJ57904.1"
                     /translation="MKCITKNGVTRLYFYESYYKDQKSRQRLVKSLGRLDELKKSYSD
                     PVAHFKELAAEETLRQEADKNMSFTIALGETLEVNEENMKNVGYGILKILYNDLELDK
                     FCKTKIRNRSIKYDFELIFRLLVFSRILYPASKRETYRHRSIYFESLEGFSIDDVYHA
                     LDLIDEHQEALQKWIYNRSGKICKRDLSVAYFDCTNYYFDIGRPDVDIFDEKGQAINE
                     EGKAVETKYRKRGPEKNRRPDPIVQMGLLMDRNSIPIAYDLFPGNESEKLHMRPIIKR
                     VKTEFSDSRIIFVADRGLNTSDNIYYLNGDNKRENNQRDGYVYGQSIRGADAEFKAWV
                     LTGGYKKTTFKDDTGEEIIFTHKSRIYPKTLKVNLTVPGSKKKKKKTISVDQKQMVYY
                     SEKYAKKQRMDREVMIKRAINMIQYPKRYDKVTSAGSAAYVKNIAFDKTTGEIIEGKI
                     LELNLEKIEEEEKYDGYYSIVTSELNMSDIEMREVYSGLSQIEETFKISKSDFKSRPV
                     HVRTNEHIDAHFATCFTALVLIRLLQAKLKNKYPVKQILNSLRKYNCTNIDTNIWQFL
                     YYDEILQKCETVFDLELNKKYRSQLQIRRLLRY"
     gene            2405..3277
                     /gene="pdxS"
                     /locus_tag="VFD89_06650"
     CDS             2405..3277
                     /gene="pdxS"
                     /locus_tag="VFD89_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HZJ57905.1"
                     /translation="MNRNYEQYKPVLEKLMGGVIMDVTTPEQAKIAEDAGACSVMALE
                     RIPADIRAVGGVSRMSDPKMIRGIQETVSLPVMAKVRIGHFVEARILEAIGIDFIDES
                     EVLSPADDKYHIDKNTFKTPFVCGAKNLGEALRRISEGAAMIRTKGEPGTGDIVQAVR
                     HMRSINQEMAALKGLREDELYFFSKELQVPYELAKYVHENGALPVPNYAAGGVATPAD
                     AALMMHLGADGVFVGSGIFKSGDPAKRARAIVEAVANYKDFKRVAELSEDLGEAMVGI
                     NEDEIEVMMANRGI"
     gene            complement(3411..3686)
                     /locus_tag="VFD89_06655"
     CDS             complement(3411..3686)
                     /locus_tag="VFD89_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HZJ57906.1"
                     /translation="MDLGIPFGVVIGYAAGLVLLYLVGWLLLVPLKMLLKFIYNGILG
                     GIILWILNLVGGLIHVTVAVNPITALIVGFLGVPGIILILLLQFILE"
     gene            complement(3755..3994)
                     /locus_tag="VFD89_06660"
     CDS             complement(3755..3994)
                     /locus_tag="VFD89_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaL family protein"
                     /protein_id="HZJ57907.1"
                     /translation="MVEDARDQAEFLAILAQARSEWLQAQNYFEIVSDPDLVDYAIYQ
                     LEAAKLKYMYLIKQARILGFENKQVIQEVPRQMFN"
     gene            complement(4283..4687)
                     /locus_tag="VFD89_06665"
     CDS             complement(4283..4687)
                     /locus_tag="VFD89_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57908.1"
                     /translation="MRIRNEIIVLIIVLASFAPLLMGCSSGGYAKEIIGEWKVDDLKF
                     KNDSDDMIADLQKMLIGIVFEPDSIIEFINKEKVSLMMNSVNYNWIEEGKLQIGDEDG
                     GDTPLFFDVTIDSDDMTFDNQMVTVYLNKVLQ"
     gene            complement(4798..6279)
                     /gene="araA"
                     /locus_tag="VFD89_06670"
     CDS             complement(4798..6279)
                     /gene="araA"
                     /locus_tag="VFD89_06670"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HZJ57909.1"
                     /translation="MADLKKYEFWFVTGSQHLYGEETLIQVKEHSEIIARALDKSQGI
                     GGNVVFKGVVTTSSEITRIMEEANFHKNCAGVITWMHTFSPSKMWIGGLSILNKPLLH
                     FNTQFNREIPWGEIDMDFMNLNQSAHGDREHGFIGARLRIPRKVSVGYWEDEEVHKTI
                     GDWMRSAIGLGESRSLRLARFGDNMREVAVTEGDKVEAQIKLGWSVNYHPVGDLVEYI
                     DGVSDNEVADLAEEYRDLYDINTPYMDSVNYQARIELGMTKFLEEGEFGAFTTTFEDL
                     HGLRQLPGLAVQRLMAKGYGFGGEGDWKTSALTRVMKVMAAGLPGGTSFMEDYTYHLE
                     KDNELILGAHMLEVCPSIAAGKPTVEVHHLGIGNRENPARMVFEGREGDAILASLIDM
                     GGRMRLIVNDVKAKSPLRDMPKLPVAGVMWKPMPSLKTSAEAWILAGGAHHTVMSYAL
                     TAEHMRDFAEMAGIEFLHINKDTDIVEFKKELLWNDMVWKFNN"
     gene            complement(6341..7048)
                     /gene="deoD"
                     /locus_tag="VFD89_06675"
     CDS             complement(6341..7048)
                     /gene="deoD"
                     /locus_tag="VFD89_06675"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HZJ57910.1"
                     /translation="MSLPTPHNKARMGEIAPTVIMPGDPLRAKFIAENYLEDARCFND
                     IRGMLGFTGFYKGLRVSTMGHGMGIPSVGIYTYELFNFYGVENIIRVGSAGGIRDDVK
                     LRDIVIAMGACTNSNYALQYGLPGAFAPIADYSLLERAVSTAVQKDLEIKVGNIFTSD
                     TFYDDSESLGKWKQMGVLAVEMEAAALYMNAAKAGKNALCICTISDLPFTGEACTSEE
                     RQTTFTQMIELALDIAE"
     gene            complement(7227..8399)
                     /locus_tag="VFD89_06680"
     CDS             complement(7227..8399)
                     /locus_tag="VFD89_06680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZJ57911.1"
                     /translation="MDNFTFYSPTYFVFGKGTENEAGHYVSRFGGTKVLVHYGGGSVK
                     RTGLLDRVIASLEASEIPYVKLGGAMPNPRSGLVYEGIDLCRKEGVDFVLAVGGGSAI
                     DSAKAIASGVKYDGDFWDFYSKDMLITEALPVGTILTLAAAGSEGSSSSVITHEDGML
                     KRGASGDAIRPVFSILNPELTFTLPPEQTANGVADIMAHVFERYFTNTKDVEVTDRLC
                     EAVLLTMIKESPKAIENPEDYSARANIMWAGMVAHNNLCGVGREQDWSSHGMEHELSA
                     LYDVAHGAGLAVMFPAWMKYVMKHDVNRFAQIAVRVWGCHMDYVNPEVTALEGIKRLE
                     EFWKSLGLPLNFKELGAKEEDIPFMVKNIGLGEEEHIGSFVPLYNDDIAKIYRLAL"
BASE COUNT         2401 a         2094 c         1702 g         2588 t
ORIGIN      
        1 gtgtatatta tattgtataa taatgggaat gtcaatgggg tgaacaaatt attttttggg
       61 gaaccgaaaa gatatatgtg tacgcatatt atttttgtaa aacttgtgag tttgtgatat
      121 tctaatacaa taatatgaag agttgcaatt gggaaaaaca gataaactat acgacataat
      181 catgttcaac gcaaaaggat agagatctca agttttgaag tgaaatcgtt aaaaggccag
      241 caaccatcag atttagtgga ctttgcctac ctgtggtatc taagtatatt ttaggaaagt
      301 ttaaaataat ttttaaatta ttaataccgt agtaagcgtc gaatttgcag ctggctacgg
      361 tattttttat taagttcaag atcaaatacc gtctcgcatt tttgtagtat ttcatcatag
      421 tagaggaatt gccagatgtt agtatcaatg ttcgtgcaat tgtatttacg taatgaattt
      481 aggatttgtt tcacgggata tttgtttttt agtttcgcct gcaacagacg aattaacacc
      541 agtgctgtaa agcaggtggc aaaatgtgcg tcgatatgtt cgtttgtgcg tacgtgtaca
      601 ggtctggatt taaaatcaga tttagaaatc ttgaacgttt cttctatctg agaaaggccc
      661 gagtatacct cgcgcatctc tatgtcactc atattaagtt cactggttac gattgaataa
      721 tatccatcat acttttcttc ttcttcaatc ttttctaaat taagctctag tatttttcct
      781 tcaataattt caccagtggt tttatcaaag gcgatatttt tcacataagc tgccgaacca
      841 gcagaagtta ctttgtcata ccttttggga tattggatca tattgattgc ccgtttgatc
      901 ataacctccc tatccatacg ctgctttttt gcatatttct cagagtagta aaccatttgt
      961 ttttggtcaa cactaatggt ttttttcttt ttctttttgc tacctggtac cgtaaggttg
     1021 acttttaagg tttttggata aatacgtgat ttgtgtgtaa atataatttc ttcccccgta
     1081 tcatccttaa atgttgtttt tttgtatcct ccggttaaaa cccaggcctt aaattcagca
     1141 tctgcacccc gtatggattg gccatagacg tatccatccc gttggttgtt ttcccgttta
     1201 ttgtccccat tgaggtaata aatattgtca gatgtgttaa gcccacgatc tgcaacgaat
     1261 attatccggc tatctgaaaa ctctgttttt actcgcttaa taattggacg catatgtaat
     1321 ttctcggact cgtttcctgg aaaaagatcg taggcaatgg gaattgaatt cctgtccatt
     1381 agaagtccca tctgtacaat cgggtctggc cgtcggttct tttcaggacc acgtttacgg
     1441 tatttagttt ctaccgcctt cccttcttcg tttatagcct gtcccttttc atcgaatatg
     1501 tctacgtccg ggcgaccaat atcgaagtag taattcgtgc aatcgaagta agcaaccgat
     1561 aagtcgcgct tgcaaatctt gccggaacga ttgtaaatcc atttttgaag tgcctcttgg
     1621 tgttcatcaa taagatccaa agcatgatat acatcatcaa tagaaaatcc ttcaaggctt
     1681 tcgaaataga tgctcctatg cctataggtt tccctttttg aagcaggata gagaatgcgg
     1741 gaaaaaacaa ggagacgaaa aattaattca aagtcatatt taattgaccg gttgcgtatt
     1801 ttcgtcttac agaacttatc aagttcaaga tcattgtaca gtatctttag gattccatat
     1861 cctacatttt tcatattctc ttcgttaact tctaaagtct cccctaaggc tatggtgaac
     1921 gacatattct tgtctgcttc ctgccgtagg gtttcttctg ccgctagctc tttaaaatgt
     1981 gccactgggt cagaatatga tttcttgagc tcatcaagac gcccaagact tttaaccaga
     2041 cgttggcgtg atttctgatc tttgtagtaa gattcataga aatacaaacg agtgacgccg
     2101 ttcttagtaa tacatttcaa aaacataata tcacctcact acaatagtat atcatgtttt
     2161 aatatatacc acaatatacc acatgtagga atattcaacc cttgacataa aaatatccag
     2221 tagccataat ggtttctgga ttaaaaattg atattaaact tcaaaactca ggattttaac
     2281 accaatccat aaacaaattc aagagtctac aaaatagttt aaatttttat cccggtgaat
     2341 tattcaaata tcctattttg tggtatagta tatattgcaa tataattttt ttgggagtga
     2401 ttagatgaat agaaattatg aacaatataa acccgttctg gaaaagctca tgggcggggt
     2461 gataatggat gttactaccc cggaacaggc gaagatagcc gaggatgccg gtgcctgctc
     2521 ggttatggct ttagaaagaa tccctgcaga tattcgtgca gtaggcgggg tttccaggat
     2581 gagcgatccc aagatgatca ggggcataca ggaaactgtt tcacttccgg taatggcaaa
     2641 ggtacgtata ggtcactttg tagaagcgcg gatattggaa gctatcggta ttgactttat
     2701 cgatgagagt gaagtactgt ccccggcgga tgataagtat catatcgata agaatacctt
     2761 taaaactccc tttgtgtgtg gtgccaaaaa tctcggtgag gccttgagaa ggatcagcga
     2821 aggcgccgca atgatacgga ccaaaggtga gccgggaacc ggagacatag tccaggctgt
     2881 tcggcatatg aggtcaataa accaggagat ggcggcctta aaggggcttc gggaggacga
     2941 gctctatttc ttttccaagg aacttcaggt cccttacgaa cttgcaaaat atgttcatga
     3001 aaacggagcg ttgcccgtac ccaattatgc ggccggcggt gtagcaaccc ctgcagacgc
     3061 ggcccttatg atgcatttag gtgcagacgg tgtgtttgtc gggtccggaa tatttaaatc
     3121 cggtgatcct gccaagaggg ccagggccat agttgaagca gtagccaact acaaggattt
     3181 caaacgcgtt gccgagcttt ccgaagatct tggagaagcg atggtaggga taaatgagga
     3241 tgaaattgaa gttatgatgg caaatcgggg aatataataa agggccggga taaaagcatc
     3301 tttgggccta tatctactaa gcggaacaag ccatggattt ataaatccca tgcccctagg
     3361 ggattatggg aacaataaat ccatggcttg ttgaatattc tactggtttt ttattccaat
     3421 atgaattgaa gcaacaaaat caatattatt ccgggtaccc ccagaaaacc gactattaaa
     3481 gcggttattg gattgacggc tacagttaca tggataagtc cccccactag attcaaaatc
     3541 cacaggataa tgccccctaa tatcccatta tatatgaatt tgaggagcat ttttagcggc
     3601 actaaaagta gccagcctac caagtagagg aggaccaaac ccgctgcata accgataaca
     3661 acaccaaagg gaattcctaa atccataccc cagaccccat ttcgcaaatt ggttcactaa
     3721 atgtatatgg ataggcattc ccttttatga cgtattaatt aaacatttgt ctgggtacct
     3781 cttgaataac ctgtttattt tcaaacccca atatacgtgc ctgcttaatt agatacatgt
     3841 actttagctt cgcggcctcc agctggtata tagcgtaatc tacaaggtcg ggatcgctga
     3901 caatttcaaa atagttctgt gcctgcaacc actcacttcg ggcctgggcc agtattgcca
     3961 aaaattcagc ctggtcccta gcatcttcca ccatagttac aacctcccct tataccaaga
     4021 tttatttcga tccatccatt accatatata tgccctacaa taaacatcaa tcccttccct
     4081 ttgtatcata gagcatttcc atcggtatat atgttgtgaa acaccttgcg ttaatggcaa
     4141 tgggacggct taacctatat aaacatcagg tccatctcac aacataccca atctatattg
     4201 ttaccattat tgtgtattct aaacttcgtt ctatggatgg caaatagata taacgggagc
     4261 gttggggctc tttttcgccc tattattgta ataccttatt tagatatacg gtaaccatct
     4321 ggttatcaaa ggtcatatcg tcactatcta ttgtaacgtc aaaaaataaa ggcgtatcgc
     4381 cgccgtcctc gtccccaatc tgtagtttcc cctcttcgat ccaattataa tttacgctgt
     4441 tcatcatgag gcttaccttt tctttgttaa tgaattctat tatgctgtcc ggttcaaata
     4501 ctatacctat aagcatcttc tggagatccg caatcatatc atcgctgtca tttttaaact
     4561 ttaggtcatc gactttccat tcccctataa tctcctttgc ataacccccg gacgaacatc
     4621 ccattaatag cggagcaaat gatgccaaaa caatgattaa tacaataatt tcgttcctta
     4681 tcctcacttt ataccctcct aaaattaaaa tcacattcta cacaatgtag catattatag
     4741 catatcatgt agaatgtgac cataaatttc cgtctagggt atcaactctt atgttcttca
     4801 gttattgaac ttccatacca tatcgttcca caggagttct tttttaaatt caacgatatc
     4861 ggtgtccttg ttgatatgaa ggaattctat tcctgccatt tcagcaaaat ccctcatatg
     4921 ctctgctgtt aaggcatagc tcattacggt gtgatgggcg cctcccgcca gtatccaggc
     4981 ctctgccgaa gtcttcagac tgggcatagg tttccacatc actccagcca cagggagttt
     5041 aggcatatcc ctaagggggg atttggcttt tacatcgttg actatcaggc gcatgcgccc
     5101 acccatatct atcaaagaag cgaggatggc atccccttct cgcccctcaa agaccatccg
     5161 cgcgggattc tcccggttac ctatccccag atgatggact tcaacggtgg gttttccggc
     5221 agcaatggaa ggacaaacct ccagcatatg ggcacctaag ataagttcat tgtccttttc
     5281 cagatgatat gtatagtcct ccataaatga tgtgcccccc ggaagtccgg cagccattac
     5341 cttcatgacc ctggttaggg cagaggtctt ccagtccccc tcgcccccga agccgtaacc
     5401 cttggccatc aatctttgaa cggccagtcc ggggagttgc ctaaggccat gcaaatcctc
     5461 aaaggtggtg gtgaatgccc caaattcccc ctcttctaaa aactttgtca tacccagttc
     5521 tatcctggct tggtaattta ccgaatccat ataaggggtg ttaatatcgt acagatcacg
     5581 atattcttct gccaaatccg ctacttcatt atccgaaacc ccgtctatat attctacgag
     5641 gtcgcctacc gggtggtaat tgaccgacca acccaattta atttgcgctt cgaccttatc
     5701 cccctcggta acggccactt ccctcatgtt atcaccaaag cgagcaagcc ttaaggaacg
     5761 ggattcgccg agacctatgg ccgatctcat ccagtcccct atggttttat ggacttcctc
     5821 atcctcccag tatcctactg aaacctttct tgggattctt aatcttgccc caatgaaccc
     5881 atgctccctg tccccatgag ccgattggtt gagattcata aaatccatat caatctcgcc
     5941 ccaaggaatc tccctattaa attgggtgtt aaaatgcaag agcggcttat tcaatataga
     6001 aagcccgcct atccacatct ttgatggtga aaaggtatgc atccacgtaa tcacaccggc
     6061 acaattttta tgaaagttag cctcctccat tatcctcgta atctcggagg acgtggtgac
     6121 tacgccttta aagaccacat tgccgccaat cccttgggac ttatccagcg cccgggcaat
     6181 gatttcggaa tgctccttta cctgtatcaa ggtctcctcc ccatagaggt gctggcttcc
     6241 cgtgacaaac caaaattcgt atttttttaa atccgccatt gtatatccct cctcaaatta
     6301 gttatatgat tatcgactcc agggtaatat tccaataccc ttattctgca atatcgagtg
     6361 ccagctcaat catctgggta aaggtggtct gcctttcctc gctggtacat gcctcccccg
     6421 taaagggcag atccgatatg gtacagatac acagggcatt ctttcccgct ttagccgcat
     6481 tcatataaag ggccgcggcc tccatttcaa cggccaaaac gcccatctgc ttccatttac
     6541 ccaggctttc agaatcatca tagaaggtat ccgaggtaaa gatgttacct acttttatct
     6601 ccaaatcctt ttgtacggcc gtactaaccg ccctttctaa aagagaataa tcggctatgg
     6661 gcgcaaaggc gccgggaagt ccgtattgga gggcataatt tgaattggtg cacgccccca
     6721 tggcaataac gatatcccgt aacttcacat cgtcccttat tccccctgcc gatcccaccc
     6781 gtatgatgtt ctcgactcca tagaaattga acagctcata ggtatagatt cccaccgagg
     6841 gtatgcccat accatggccc atggttgaaa cccttaaccc cttataaaat ccagtaaaac
     6901 ccaacatgcc ccttatatcg ttaaagcatc tggcatcctc taaataattt tccgcgatga
     6961 acttagccct taaaggatcc cccggcatta ttacagtcgg agctatttcc cccattctcg
     7021 ctttattgtg aggtgtcggt agtgacacgg caattctcct ctccataaga gttattttta
     7081 tatgtcaaag cgtaggggcg acctaaaaag tcgcccctac aaattacacc ctgttgttgt
     7141 ctgatcccgt ggttaatagg tccttgaatc cccagaggca gccccctatg cttttctgtt
     7201 tttcccggga cttgatcccc aacccgttat agggccagcc tatatatctt cgcgatatca
     7261 tcgttataca ggggaacgaa actgcctata tgctcctctt ctcccagccc tatgttttta
     7321 accataaagg gaatatcctc ttccttggca cccaactctt taaagtttaa ggggagaccc
     7381 aatgatttcc aaaactcctc taatctctta atgccttcta aagccgttac ttcaggatta
     7441 acataatcca tatggcatcc ccagacccga actgcgattt gggcgaagcg attaacatcg
     7501 tgcttcataa catatttcat ccatgcgggg aacattacgg ccaggcctgc accatgggct
     7561 acgtcataga gggcggagag ttcatgctcc attccatggc tgctccagtc ctgttcacgt
     7621 ccaaccccac acaggttatt atgcgctacc ataccggccc acataatatt agccctggcg
     7681 ctataatcct cgggattttc tatagctttg ggcgattcct ttatcatggt caacagcacc
     7741 gcctcacaaa gcctgtcggt aacctccacg tccttcgtat tggtgaaata acgttcaaag
     7801 acatgggcca ttatatcggc aacaccgttg gcggtttgct ccgggggcag ggtaaaggtt
     7861 aattccggat ttaagatgga aaagacgggg cggattgcat ccccagatgc ccctctcttg
     7921 agcatcccat cctcatgggt tatgacggaa ctggaagaac cttcactgcc cgcggccgca
     7981 agggttaata ttgtacctac gggaagcgcc tctgtaatga gcatatcctt ggaataaaaa
     8041 tcccagaagt ctccgtcgta ctttactccg gaggcaatag ccttagccga atcgatggcg
     8101 ctacccccgc cgacggccag aacgaaatcc acgccttcct ttcggcacag atcgatacct
     8161 tcatatacca aaccgcttct cggatttggc atcgcaccgc ccagcttgac gtagggtatc
     8221 tcggaagcct ccaatgatgc tataactcta tccaacagac ctgtcctctt tacagacccg
     8281 ccaccgtagt gaacaagcac cttggttccg ccaaaacggg atacataatg tcctgcctcg
     8341 ttttcagtac ccttaccgaa cacaaagtat gtaggactat aaaacgtgaa attatccatg
     8401 caacaccctc ctaaatagat tctccattta ttgttgtaat ataattatac actattattg
     8461 atgtgctgac acatcaaagt cgtatttatt gggacataat cgtattaacc cagccgttat
     8521 atggtaaaag aacatatctc ctatggccat ccccgccatt tctccaaaat ttagggggta
     8581 taaccgtcca tgcggatata cccccacctc tataaaacat tggctttaca gcctaccacg
     8641 taccctgaac ctcccatggc taaagtcacg gggttctaga gttcataaga actcatttac
     8701 gttcatagca gctaagctac tttgatacgt acaggcgtgt ccagttcgcc tctaccgtat
     8761 agccctgtta agggcacaac gctac
//
LOCUS       DASRNX010000168         4836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_511813,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000168 DASRNX010000000
VERSION     DASRNX010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511813"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..1513
                     /locus_tag="VFD89_06685"
     CDS             314..1513
                     /locus_tag="VFD89_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZJ57912.1"
                     /translation="MEKIIETREVPFIGEYDIVIAGGGIAGVAAALAAKRLGSKVLLV
                     EKSVMLGGLATLGLISWYEPLCDGNGRHIIKGIAEELLLLSIKYGFNDLPDHWRTGEG
                     KDSSLRRYATHFSPTIFALALNDLMKTEGIDVRLDMLASYPIMEQNRCKGLTVESKSG
                     REFFKAKVLIDATGDADILFRGGVPCVQGENFLSYVAHGFGAKDAQKYMEAKDALNIR
                     RWIACASNLNGVGHPEGLGKFSGTTNEDVTEFVQLGQQILFDKIKDDPPRERDIITIP
                     GMAQYRTTRHIQGEYELTIRDEGKHHGDSVGAIGDFRKRNVHFEIPYGCLYNSEFPNM
                     LAAGRVVSASGDGWEVTRVIPVAALTGQVAGSAAHLASTKNQTVADIDVAFLQGLLAE
                     QEIVLHF"
     gene            1775..2050
                     /locus_tag="VFD89_06690"
     CDS             1775..2050
                     /locus_tag="VFD89_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HZJ57913.1"
                     /translation="MSLTEGQWTYVILINIIAFILMGIDKAKARRGAWRISERTLIIL
                     AVIGGSLGMLFGIHMFRHKTKHAKFKLGIPFIIVLQILLFFLWKMVI"
     gene            2204..2368
                     /locus_tag="VFD89_06695"
     CDS             2204..2368
                     /locus_tag="VFD89_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57914.1"
                     /translation="MYNSKNQVIKTIKLEGPDRSVFDLPPEYGRDFAWIGMYPSPDDR
                     PSKGVSGGRG"
     gene            2549..>4836
                     /locus_tag="VFD89_06700"
     CDS             2549..>4836
                     /locus_tag="VFD89_06700"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HZJ57915.1"
                     /translation="MRLLYNKPAEKWVEALPLGNGRIGAMVFSIPDRDVFMLNEDTLW
                     SGYPRDTNVADAAEYNPKALELVKQKKYKEAEECIEDHMLGGYTQSYMPLGDLRLDFP
                     SIDRENVTSYSRELNLNNAIVTTSFFHKGVRYKREAFVSYPDQCMIIRLSADKGQSIS
                     FSASFSSQLKSATAVEGNTALLDGICPSHVEPNYINSHNPIIYEKEDHKKGIGFRAAA
                     IIENHGGKVVAKDNSIHVEDANSVVIKLCVRTSFNGYDKQPFLEGKDYKRDCQDDHDK
                     IQGQPFDILKARHVEDYQELFNRVLLNLDSDKKDCIPTDRRLELFQETQDDKGLYELI
                     FQYGRYLLIASSREGTQPANLQGIWNKEMRPPWSSNYTLNINTEMNYWPAEMCNLSEL
                     HQPLFDLIDGLRVTGAKTAKLHYGAGGFTAHHNTDIWRLSNPVGDFGRGTAGYAFWPM
                     AAGWLCRHLFEHYEYTLDKEFLEYRAYPAISDAATFYMDMLVEDEEGYLIMSPTTSPE
                     NAFIYGGEICKVSKTATMTIAIIKEVFANFIKCCEALSIEDEMSRGIRDKISRLYPYR
                     VGSKGQLLEWEEEFQEPEPHHRHISHIYPLYPGFEITRLETPDLAEACRKTLNIRGDD
                     GTGWSLGWKINTWARLHDGNRALKLLKRQLRYVSESNTDYMGGGGTYANLFDAHPPFQ
                     IDGNFGACAGIAELFLQSYDDKVLILPALPDELREGRIKGLCAKGGIVTDITFYNGGL
                     EKMDFYTTSGESRQVTFIYKDR"
BASE COUNT         1440 a          904 c         1239 g         1253 t
ORIGIN      
        1 acggtcccgc tgtgttaaca cggcggaacc gaaggacaat aaaataaaga gcgccgagaa
       61 aagcttcata taagcccaaa gagccgaaag ggaaagacat tgacacgaaa agccacattg
      121 tttttcgggg taggccctca gccacaaaag tgactacata atttggttgt gtatcaaaca
      181 atgtgttttt gtgacatatg aattaatgat agcgtttttg gcgtttggtt atcaatgtaa
      241 aagggctatc gtgtaatatg taggttgatt tctactgcgg tatccaaaat caaaaatcca
      301 aggtggggaa ataatggaga aaattataga aacgagagaa gtgcccttta ttggagaata
      361 tgacatagtt attgcagggg gcggtatagc cggagttgcg gcggctcttg ccgcaaaaag
      421 attgggtagc aaggtcctat tggttgaaaa aagcgttatg ctcggtgggc ttgcgaccct
      481 agggttaata agttggtacg aaccgctttg cgatgggaat ggaagacata ttattaaagg
      541 tatagccgaa gagctattgc tattgtccat taaatatggg tttaatgatt tgcctgacca
      601 ttggagaacc ggggagggga aagactctag tttgagaagg tacgctaccc acttttcccc
      661 tactattttt gctcttgccc ttaacgatct tatgaaaaca gagggaatcg atgtaagatt
      721 ggatatgtta gcatcatatc caataatgga acagaacagg tgtaaagggc tgacagtgga
      781 gagtaagagc ggaagggaat ttttcaaagc caaggtatta atcgatgcca caggagatgc
      841 ggacatttta tttcgtgggg gagtgccatg cgttcagggc gaaaattttt taagctacgt
      901 cgcccacgga tttggggcaa aggatgcaca aaaatacatg gaagctaagg atgccctaaa
      961 catacgaagg tggatagcat gcgcctctaa tttgaatgga gtaggccatc ctgagggttt
     1021 aggcaaattt tccggtacaa ccaatgaaga cgtgaccgaa tttgtccagc ttgggcaaca
     1081 gatattgttc gataaaatta aagatgatcc gccaagggaa agggatatta ttactattcc
     1141 gggtatggct caatatcgga ctactcgcca tattcaagga gaatacgagc tgacgattag
     1201 ggatgaaggg aaacatcatg gggactccgt aggggccatt ggagattttc gaaaaagaaa
     1261 tgtccatttt gaaattccct atggctgtct atataatagc gaatttccaa atatgctggc
     1321 tgcaggaagg gttgtcagcg ccagcggcga tgggtgggag gtcacccgtg ttattccggt
     1381 tgctgccctc acagggcaag tagcaggtag tgccgctcac ctggctagca caaaaaatca
     1441 gactgttgct gatatcgatg tggctttctt gcagggtttg ctcgccgaac aggagattgt
     1501 actccacttc tagtgccaag gtgacgtttc aactgtcatc aaagcggata ggaagcatgt
     1561 gaaaaggcaa gctagggacg actgcccgag gcttgcacaa ggctgagaat tggaatggta
     1621 ctaaatgctg aagaatcatt gcgggttggc acaaaaaatg ctaaaatata actatcaaca
     1681 aggcaaatgg accaaagggt tttttgcctt gtttttatat agaatgattc agctgttcat
     1741 cagaagaaag atagcttact ttaggaggca aactatgagc ctaactgaag gacaatggac
     1801 ttatgttatt ctcatcaata ttatagcgtt tattctaatg ggcatcgaca aagcaaaggc
     1861 tcgcaggggg gcatggagaa tatcggagag aacccttatt attttggctg ttataggggg
     1921 atctttgggc atgttgtttg gtatacatat gtttcgccac aaaaccaaac atgcaaaatt
     1981 caaattaggg atcccattca ttatagtttt acagatactg ttatttttcc tatggaaaat
     2041 ggtcatctaa acagcatcta ataagtggag tgccaagaag acatttcatt tgtcatcaaa
     2101 tgatgccagg ggatgtttcg tttgtcatcg ctttggctga tgtagaggct cggaaggcgc
     2161 agttcgggag atttgacttt attatttaag gggtgctgta taaatgtaca atagcaaaaa
     2221 tcaggtaatt aaaacgataa aattggaagg acctgatcgt tcggtctttg accttccccc
     2281 agaatatggc agagactttg cctggatagg catgtaccca tctccggatg acagaccgtc
     2341 aaagggtgtg tcagggggac ggggttagtg acacaatttg ccatgggatt gccggattgc
     2401 catggggatt ttgggaagtc tgtccgttct tggtatggca gattaataag aaatggtgat
     2461 tgaaagcgtg tcattaaccc cgtccctgcg acacaccgac atcgtccctg cgacatacgt
     2521 cacaataata taggaggtat acaataagat gagacttttg tataataagc cggcggaaaa
     2581 atgggtagag gcgctgcctc tgggtaatgg caggataggt gcaatggtat tttccattcc
     2641 ggatagggat gtttttatgc ttaatgagga taccctctgg tcggggtatc ctagggatac
     2701 aaatgtagca gacgccgcag agtataaccc gaaagccctt gaacttgtaa aacagaaaaa
     2761 gtataaagaa gccgaggagt gtatagaaga tcatatgctg ggcggataca cccagtccta
     2821 tatgcccctt ggggatctta ggctggactt cccttctatt gatagggaaa atgttacatc
     2881 ctacagccgg gaactaaatc tcaataacgc aatagtaact acaagctttt ttcataaagg
     2941 ggttcgatat aaaagagagg cctttgtatc ctatccggac caatgcatga ttataaggct
     3001 ttccgcggat aagggccaat ctatcagctt ttctgcatca tttagcagcc agcttaaatc
     3061 cgctactgcc gtggagggca acactgcact attagatgga atatgtccgt cccatgtgga
     3121 accaaactat ataaattccc ataatccgat tatatatgag aaggaagacc ataagaaagg
     3181 gataggcttt cgggcggcag ctattattga aaaccatggt ggcaaagttg tggcaaagga
     3241 taatagtatc cacgttgaag atgcaaatag cgtcgttata aagctttgtg ttcgcaccag
     3301 ctttaatggc tatgataaac agccctttct agaaggtaag gattacaaga gggactgcca
     3361 agatgaccat gataaaattc agggacaacc atttgatatc ttaaaagctc gtcatgtgga
     3421 agactatcag gaattattca atcgggtcct tcttaatcta gactctgaca aaaaggattg
     3481 tattccaacg gatagacgat tggagctttt tcaagaaacc caggacgata agggcttgta
     3541 tgaactcatc ttccaatatg ggcgttatct attgattgcc agctccagag agggcactca
     3601 gcctgcaaac ttacagggga tatggaataa ggagatgcgt ccgccctgga gcagcaatta
     3661 tacgttgaat ataaacactg agatgaacta ttggcctgca gagatgtgca atctctcaga
     3721 actacaccag cccttatttg atctaataga tggactccga gtaacagggg caaagactgc
     3781 gaagctgcac tatggagccg gaggttttac ggcacatcat aacacagata tatggagatt
     3841 atccaatcca gtcggggact ttggccgggg gacggcagga tacgccttct ggcccatggc
     3901 cgcaggatgg ctatgtcggc atctatttga gcattatgaa tatacattgg acaaggagtt
     3961 tttagaatat agggcatacc cggccattag tgatgctgcg acattctaca tggatatgtt
     4021 ggtagaggat gaggaaggct atcttattat gtctcccacc acttcaccgg aaaacgcgtt
     4081 tatctatggt ggggaaatct gcaaggtttc aaagaccgcc actatgacca tcgctatcat
     4141 aaaggaggta ttcgctaact ttattaagtg ctgcgaagct ctaagtatag aggatgaaat
     4201 gagccggggt ataagggaca agatttcaag gctctaccca tatagggttg gatcaaaggg
     4261 acaactttta gaatgggaag aggagtttca agagccggag ccacatcata ggcatatttc
     4321 ccacatctat cctctatatc caggctttga aataacccgg ctggaaacgc cggatttggc
     4381 tgaggcgtgc aggaaaacct taaatattcg gggggatgat ggtacaggat ggagccttgg
     4441 gtggaagata aatacttggg ccaggcttca tgatgggaat cgtgcgctaa aacttttaaa
     4501 gcgccagcta cgatacgtca gcgaaagcaa taccgattat atgggcggcg ggggtactta
     4561 tgccaatctc tttgatgccc atcccccttt tcaaattgac ggaaactttg gagcctgtgc
     4621 gggaattgcc gaattattcc tgcaaagcta cgatgataag gttttgattt tacccgccct
     4681 gccggatgag cttagagaag ggcgtataaa gggcctttgc gcaaagggcg ggatagtaac
     4741 cgatatcaca ttctataatg gcgggctaga gaaaatggat ttttatacta catcgggaga
     4801 aagcaggcag gttacattta tctataagga tagata
//
LOCUS       DASRNX010000169         4072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_86301,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000169 DASRNX010000000
VERSION     DASRNX010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86301"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..748)
                     /locus_tag="VFD89_06705"
     CDS             complement(308..748)
                     /locus_tag="VFD89_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZJ57916.1"
                     /translation="MDIAKMKRRLRGLKKFENKIRFSHLPQGIPKKYVWDEYFSTKVI
                     HDLTVKYPILDLLKHDRQGLKEIFEEFFYSVYFQLYKDFGLDFDEIKDPTVLDHFGLA
                     PGASVEDIKKRFRELAKKHHPDHGGRDERMIEILDAYNRLLNKK"
     gene            1274..2296
                     /locus_tag="VFD89_06710"
     CDS             1274..2296
                     /locus_tag="VFD89_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZJ57917.1"
                     /translation="MKEKNLKAKAGIPMLIFFISLYFVAIGLIILGGILINAGIGIGA
                     LPLSIGLIWIIIGIVPFFGLKIIKPQEALVLTLFGEYIGTLREPGFYYVHPFAVSVNP
                     ASRTRLGQSGDVDSSPGSKLVISAEGNMVQTNTATKKISLKIMTLSNNKQKVNDVLGN
                     PIEIGIAVMWRVVDTAKAVFNVDNFKEYLSLQCDSAVRSIVRIYPYDVAKGIDTTGDG
                     EPDEGSLRGSSAVVAERIRKEIHDKVQDSGLEILEARITYLAYAPEIAAVMLQRQQAS
                     AIIDARKMIVDGAVGMVEMALDKLNENDVVKLDEERKAAMVSNLLVVLCGSKDAQPIV
                     NSGSLY"
     gene            2299..2481
                     /locus_tag="VFD89_06715"
     CDS             2299..2481
                     /locus_tag="VFD89_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HZJ57918.1"
                     /translation="MSMGKKQIPLRLSKKLYEEIAAWAEDEFRSINGQIEYLLTQCVR
                     DRKGIDKGISDDKKES"
     gene            complement(2952..3836)
                     /locus_tag="VFD89_06720"
     CDS             complement(2952..3836)
                     /locus_tag="VFD89_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZJ57919.1"
                     /translation="MKISTLTSHLAKVYGEENAIRKLARIGYDCYDFSMFGIGEEGHP
                     INSNSFESYVQKLRKAADDSGIECNQSHAPFPSYLHDDKEYNKRMFPLLVRAIEVTGL
                     LGGEIVIVHPAVFPGHKIGGDSLCWDTNMELYHALLPHAKKSNVKIALENMFNWYEGA
                     STASPATCSSPEEFVKYMDALDPEYFVACVDIGHSAMENAGGRSPAEMIRALGGKRLK
                     ALHVHDNDLVRDVHTLPFNQKIDWDEVMTALRDIGYDGELTFEADAFLSQFPNELAVH
                     ASRLMLEVGRYFVDKYEL"
BASE COUNT         1150 a          907 c          848 g         1167 t
ORIGIN      
        1 gacagattta ataccatata agaaatgaac cgctgcgctc atcactatat atggtgtatc
       61 ttgctagaaa atgtagtttt gacaccctga tcattataaa taactataat accgattgtc
      121 cgtgccgggt ataggggcgg aaggccgtgc agtaacgtct aacattgcca actgcattcg
      181 tcagagcggg gcgcatgtta atataaccgc agctattttt cgtctgttca caaacaatta
      241 cccggtatac gcccagatgg ggacgatcag tttatccaag tgactttctg cacggcctag
      301 cgggttttta cttcttattt agcaatctgt tataagcatc taatatttct atcatcctct
      361 catcccttcc gccatggtcg ggatgatgct ttttggctaa ctctctaaac ctctttttaa
      421 tatcttctac agaggcccca ggtgcgagac cgaaatgatc caagacagtg gggtctttga
      481 tttcatcaaa atccaatccg aaatccttat ataattggaa gtatacgctg tagaaaaact
      541 cttcgaagat ttcctttaat ccctgtctgt cgtgttttag cagatccaat ataggatatt
      601 tgaccgtcaa atcatgtatg acttttgtgg aaaagtattc gtcccataca tatttttttg
      661 gtattccctg cggtaaatga ctgaatcgaa ttttgttctc aaactttttt aagcccctga
      721 gcctgcgctt catctttgct atatccaaac agacacccca ccttcgaatg atctactctt
      781 aatatctgcg gaaacagagc cacccttatg atgttacccg gtgttcaata tccattacag
      841 gtgtatgttt aactgacaca atactttagt taatttttga atatagatta ctataccaac
      901 ttcagcgttg tgcgggcgac aaacaaccgt gccgaaataa tgatgcctgg cgaaagcccg
      961 gaagcctctt tgatccatta tatcgttttt atccgacgaa tcataggaga gattccgata
     1021 gaacaaattt taattgaatt tcccccataa atccgatata acgagtaggc aagatatctc
     1081 tagcttctat aagcggcgaa tttcgattag caaaataggc gatgggtata aatcccccgc
     1141 ctcgttgaaa cctgtgtgtt ttaattacca acgcaattgc ttccgatatt ttaataattg
     1201 ccattttatg cttgttaatt tgatatcatt atgatatcat aagtatatca taatgatttg
     1261 tgaggtggaa tatatgaagg aaaaaaactt aaaagccaaa gccggaatac ctatgctgat
     1321 tttctttatt tcgctctatt ttgtcgccat aggcctgata attctgggcg gaattcttat
     1381 taatgccggt atcggtatcg gtgcgctgcc cctgtccatt ggacttatct ggattatcat
     1441 tggcattgta ccctttttcg gactcaagat aataaaaccc caagaggcac tggtgcttac
     1501 cttatttggg gaatacatcg gcaccttgag agaacccggt ttttactatg tacacccctt
     1561 cgcggtgtcg gtcaatccag cctctagaac tcgtcttggg cagagcgggg atgtggatag
     1621 tagccccggc tccaagcttg ttatctccgc agaaggaaat atggttcaga caaataccgc
     1681 gactaaaaag atttcgctta agatcatgac cctcagcaac aacaagcaaa aggtcaacga
     1741 tgtcttaggc aatcccatag agataggaat cgcggttatg tggcgggtcg tcgacacagc
     1801 caaggcggtt ttcaacgtag ataatttcaa agagtatctt tccctccaat gcgacagcgc
     1861 tgtgcgaagc atcgttcgta tctatcccta tgacgtggcc aaaggcatcg atactacagg
     1921 cgatggtgaa ccggacgaag gaagtcttcg cggttccagc gccgtggtgg ccgaacgtat
     1981 ccgaaaggaa atacatgata aggtgcagga ttcgggcctt gaaatcttgg aagcccgcat
     2041 tacctacctg gcctatgcgc cggaaatcgc tgcggtaatg ttgcaaagac aacaggccag
     2101 tgccataatc gacgccagaa aaatgatagt ggacggtgct gttggtatgg tggaaatggc
     2161 attggataag ctaaacgaaa atgatgtggt aaaacttgat gaggagcgta aggccgctat
     2221 ggtatccaat cttttggttg tcctctgcgg aagcaaggat gcccagccta ttgtcaacag
     2281 tggcagcctg tactgataat gagcatggga aaaaagcaga tccctctccg cttgtctaag
     2341 aaactatacg aggagattgc cgcatgggct gaggatgaat ttcgctccat taacggacag
     2401 atagaatatc ttctcaccca atgcgtcagg gacaggaagg ggatagacaa aggcatttcg
     2461 gatgataaga aggaatccta ggacttagtg aaaaaggttg tctaccgcat gggggcggtt
     2521 gacaaccttt tttcttgtat aacgcaactt agttatcaaa aaaatataac accggaagca
     2581 attgcattgg cagttgcaac acacaggttt caacggggcg ggggatttat acccaccgct
     2641 tgttttaata atcggaactc gccgcttata gaaacgggag acatcttgcc gactcgttat
     2701 aatacagtcc tgcacaacca taaacagcct aggcagttag tattacaact atatggtcaa
     2761 atatatagtt tgtgttcttg aagttcccgt taagctatca gtattacaac tacataccgt
     2821 acgggttgtt gatgaaatgg ggttttgaca ctctaatgat aagaatatct aaatcgatac
     2881 tctgttccag cgagtattcc cttgccaaat cccggccata atatcaaaca gatcttctac
     2941 ccttctctat tctagagttc gtatttgtct acaaaatagc gtcccacttc cagcatgagc
     3001 cgtgacgcat gtaccgccag ttcatttgga aattggctga ggaaggcatc tgcttcaaag
     3061 gttagctccc catcataccc aatatcccta agcgctgtca tgacctcatc ccagtcaatt
     3121 ttctggttga agggcaacgt atgtacatcc ctcaccagat cgttatcatg gacatgtaat
     3181 gcctttagcc ttttgccccc gagggcacgg atcatttcgg ccggtgacct tcccccggca
     3241 ttttccatag cgctatgtcc gatgtcaacg caggctacaa aatattccgg atccagagca
     3301 tccatatatt ttacaaattc ctccggagaa gaacaggtag caggcgaggc cgttgaagcc
     3361 ccttcatacc agttaaacat gttttcaaga gcgatcttta cattggattt cttcgcatgg
     3421 ggaagcaagg catggtaaag ctccatattt gtatcccaac atagggaatc cccgcctatc
     3481 ttatgcccgg gaaaaacagc ggggtgaaca attacaatct ctccccccaa taatcccgtg
     3541 acctcaatag cacgaacaag gagcggaaac atccgcttat tatactcctt atcatcatga
     3601 agatagcttg gaaagggtgc gtggctctga ttacattcaa taccgctatc gtctgcggcc
     3661 ttccgtagct tttgtacata ggattcgaaa gaattgctgt ttatcggatg gccttcctct
     3721 ccgatcccaa acatggaaaa gtcatagcaa tcgtacccaa tcctagcaag ctttcgaata
     3781 gcattttctt ctccgtatac ctttgcaagg tggcttgtaa gggtagaaat tttcatttga
     3841 tccttcactc gctttctttg atattatttg aaatgtttaa ttagggaaca aaacttcttg
     3901 ttaccatatg ttttacgaca aacgtcctag ataatgttct gatgtcgtaa aacattctcc
     3961 ttggatttat atattacgaa aaatacattc atataagata ggtccagatt gtctaactga
     4021 tttgtcaata atatagataa ccaactttaa ctataaaatt tatcaccatg aa
//
LOCUS       DASRNX010000170        24146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_721201,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000170 DASRNX010000000
VERSION     DASRNX010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 24146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24146
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721201"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1563
                     /locus_tag="VFD89_06725"
     CDS             52..1563
                     /locus_tag="VFD89_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HZJ57920.1"
                     /translation="MKKAKMILDKDFKVGQVDDRIYGSFIEHLGRAIYGGIYEPEHPE
                     ADEKGFRKDVTRLVKELKMPIVRYPGGNFVSGYNWEDGVGPKENRPRRLELAWRTIET
                     NQFGTNEFVDWARQADTEVMMAINLGTRGVDAARNFVEYCNHPGGTYWSDLRRSHGYE
                     KPHNIKTWCLGNEMDGPWQIGHKTADEYGRIALEAAKVMKWVDPTIELVACGSSNIEM
                     PTFAQWEATVLDHTYELVDYISLHQYYGNRDDDTPNFLARTMDMDLFIKSVVSICDYM
                     KAKKRSKKTINLSFDEWNVWFHSNEADKKIEPWTVAPPQLEDVYTFEDALLVGSMLIT
                     MLRHCDRVKMACMAQLVNVIAPIMTEDGGAAWRQTIFYPYMHASLYGRGVVLDPVISS
                     PVYDTKEYSDVPCLDSTAVFNEEEEELTIFAVNRGMEDSLVLECDLRNFPGYRVLEHI
                     TMEHDDVKATNTRDNPYNVVPADRQDTEVADGQLTTRLKNLSWNVIRLGKSGQ"
     gene            1585..2403
                     /locus_tag="VFD89_06730"
     CDS             1585..2403
                     /locus_tag="VFD89_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HZJ57921.1"
                     /translation="MEDDKEDLTLVGKKQMLRNIKYFILDMDGTFYLGDKLLDGSLEF
                     IDTLEKTGHDFLFFTNNSSKNPDVYIDRLKGMGCTVENNKVVTSGMVTIHHLKKHHKG
                     ARVYLLGTPELEEQFINEGIELVDSNPDLVVAGFDTTITYDKLSRACTSIRQGATFIA
                     THPDFNCPTEDGFIPDCGAICAFITASTGVKPKYLGKPYTETLEFILDHLNCTADDIA
                     FVGDRLYTDIAIGVNHGVTGILVMTGEATEEDIKKSEIQPDIIVERLVDITKWL"
     gene            2718..3500
                     /locus_tag="VFD89_06735"
     CDS             2718..3500
                     /locus_tag="VFD89_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018977748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HZJ57922.1"
                     /translation="MAFIQCDFFSEVLGLSTSMNVILPQPALGQIGMEGSKASDKYPV
                     LYLLHGLSDDHTIWGRRTSVERYVASLGLAVVMPAVHKSFYTDMAKGDKYWTFVSEEL
                     PQIVRSFFPISAAREDNFVAGLSMGGYGAFKLALRCPDKFIAGASLSGAVNIAEIAKN
                     RDPGRAWENVFGNLDEIKGSDNDLLHLAEEVSKSNGPKPMLYQCCGTEDFLYEDNIVF
                     RDWANELGLNLTYEEEPGTHEWGYWDKKIQRVLEWLPMNNSK"
     gene            3900..4988
                     /locus_tag="VFD89_06740"
     CDS             3900..4988
                     /locus_tag="VFD89_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HZJ57923.1"
                     /translation="MEELNKKLKMRNLPHVLVDQEGKIINDDEAWASRRQEIVKLFGS
                     QVYGFSPPAPLKVSSKLVREDEDAFAGKAVHSIIDLSFDTPGGEFSFPVNLVIPKGVS
                     KAPLFLHIAFSPDVPDRYFPVEEIIDGGFAVASFYYEDITRDEDDDFKSGLAGMYSGE
                     NRKPDEWGKISMWAWSAQRVMDFIQTVEAIDKGKVAVVGHSRLGKTALWCAAQDERFI
                     MGISNESGCSGAAISRGKNGERIKDIVKTFPYWFCDNYKDYSDNEENMPFDQHFLISA
                     IAPRYVYVSSAVGDEWADPESEFLGCVAAGDVYELFGVKGLVTEDVLPKVGTKLHDGH
                     IGYHLRGGTHYLSRYDWQGFMEFMKRKL"
     gene            5300..7285
                     /gene="tkt"
                     /locus_tag="VFD89_06745"
     CDS             5300..7285
                     /gene="tkt"
                     /locus_tag="VFD89_06745"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZJ57924.1"
                     /translation="MKQIEKNSINAIRVLSAEMVERAKSGHPGMPMGAAPTAYSLWAR
                     QMKHSPNNPDWLDRDRFVLSAGHASALIYSLLHLFEYGLSMEDLKNFRQWDSKTPGHP
                     EYGHTVGVETTTGPLGQGFATGVGMALAEAHLAAKFNRPGYEIVDHYTYILSGDGCMM
                     EGITSEAASLAGTLELDKLIVLYDSNAITIEGGTDMAFKEDVGKRFEAYNWHVLRVED
                     GNDVDAIASAIEDAKAEVKKPSLIIVTTQIGYGSPAKQGKASAHGEPLGAENLKAAKE
                     YLKWPYEEEFFVPQDVREHMASIRDKGAEKEKAWQEKWDAYSKEYPELAREWDLWHSK
                     DLPIDLLNDEEFWSFDGKDATRSSSGKVINRLTKILPNLIGGSADLGPSNKTFMEGRD
                     SFSAENYGGSNLHFGVREHAMAAMGNGMVLHGGLRAYVATFFVFTDYMKPAMRLSALM
                     ELPLIYVLTHDSIGVGEDGPTHEPIEHLAALRSIPGFTEFRPADSKETAAGWYAALTR
                     GDSPTGLILSRQNLPLYKETGKEALKGGYILLDSEKEIPDIILMASGSEVELIYEAHA
                     VLQEQGIHARVVSMPSFKIFEEQSPEYREGVLPNMVRARLAVEAGSSFGWHKYVGLDG
                     DIIALDRFGASAPGEILFEEFGFTVDNVVSRAKALIG"
     gene            complement(7399..7665)
                     /locus_tag="VFD89_06750"
     CDS             complement(7399..7665)
                     /locus_tag="VFD89_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57925.1"
                     /translation="MLRVWGIIRKNNKILKDMVADYAGDDIREIDMLHHCIQRICYDF
                     DLQRPMWLPKNQREYEDYRRILLTQDNFIEPIDFDALELEILDD"
     gene            7874..8695
                     /locus_tag="VFD89_06755"
     CDS             7874..8695
                     /locus_tag="VFD89_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZJ57926.1"
                     /translation="MIGRFLNFIGCMYRRFLDDDILALGAQMAYYLVLSFFPFLIFLI
                     SLIGYSPISSRQVLEGLLGILPEEAYILVRDTIVEIRGTRSGGLVSVSGVLSLWFASN
                     GIGSIITGLNKAYDEREKRSFIKVKLISVFFTLLLSVAIVFSFALLVFGGVIKRRLID
                     CISLGPFHRGLWDVGRYVFISSVILLIFIILYRYTPSRRMGWREVVPGAIFSTAGWLG
                     TSTGFAYYVDNFGHYSTFYGGIGGVVILLIWLYFSAVIILIGGELNAALVYNKAK"
     gene            8971..9756
                     /locus_tag="VFD89_06760"
     CDS             8971..9756
                     /locus_tag="VFD89_06760"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HZJ57927.1"
                     /translation="MNIYIMVDAEGISGVYNSDQVMSTGSRYQEGRELMVRDINACVE
                     GCKEAGADRIYVRDCHGSGSNVVWSRLSDLADYYIIGDTGQDRFPGLDECHGVILLGY
                     HAMAGTPGAILEHTMSSEAVQNYWINGVKVGELAIDAGIVGDRGKPVIMVSGDDKVCL
                     EAKGLLPDVITAQVKKGITWRGGMLLPPQKSYNLIKEKAKEAVNLLNMQQALVFSKPI
                     RLRVEMTERGILPLQYSKPYMTVIDGRTYEVEGSTVEEALFRL"
     gene            9958..11994
                     /locus_tag="VFD89_06765"
     CDS             9958..11994
                     /locus_tag="VFD89_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57928.1"
                     /translation="MKNIVNLESFALENGEAFVLDREHALIKLNKGQGRLVYEGEPMN
                     LGEADWKDAKYLVFDVKGYEDWDIPLLIEFWNPDNKTETPTLHYIVGLLPKIRTRVSL
                     PLEALSSEEMFLTRTPVKLKTVIHGDRVYLEEANRFAISTRPSSFEQRLEITNMYLTD
                     KEPDYPVPDVVLVDELGQYTKREWPGKVHSEDEMVNYLHRELETRNKNDLSEFSDEWS
                     IYGGWKGKQFSSTGYFRTEYDGKRWWLVDPEGYAFYSNGLDCIHPHEQFSHISGIEKF
                     ASWLPEKDGKFRDAWRMSEGRWEASGEGSEEYDFAISNLIRAFGDKWFDSWAGMTKGR
                     MIDWGFNTVANWSDAGFIRFAKMPYVWPMRDFPETEKKIFRDFPDVFSDEYKVNALRY
                     AQQLREFEGDRNMIGYFLRNEPIWAFVDMNIAEKLLENEHMTDTKAELIKTLSDKYSG
                     DIAGFNAAWNLDLDSFGELKNSIRGATRLSPRALEDLEEFSRVMIERYVKIPSDAVKR
                     VDPHHLNLGMRYGTLSRDNLAAGCENFDVFSINCYSMNPKDDLIKAGTLTGMPVMIGE
                     YHFGALDRGLMATGLRAVSSQSERGKAYRYYCENAAATKYCVGTHYFTLGDQSFLGRG
                     DGENYQIGIVNVCNRPYEEIIGGMIETHKRIYEVAEGTREAYNTRPDEIGFIAY"
     gene            12274..13590
                     /locus_tag="VFD89_06770"
     CDS             12274..13590
                     /locus_tag="VFD89_06770"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZJ57929.1"
                     /translation="MGIKVAKFGGSSLADADQFRQVRSIVMMDEGRRYVIPSAPGKRH
                     SGDHKITDLLYLCQEHAQKGVPLGEIFELISTRYLQIVKELELFLDLKPILEDIKDKV
                     LGGYSVEYTASRGEYLNGLILAEFLGYEFLDPADIIFFTQSGIYDEQRTQEAVEKALA
                     DIPRAVIPGFYGSRPDGGIQTFSRGGSDITGAIIARGIKADLYENWTDVSGFMMADPK
                     IVKNPRPIEEITYRELRELAYMGAKVLHEDAVFPVRQARIPINIKNTNEPEAVGTLIV
                     PNGGEFARAGMITGIAGNKDFTVITVEKAMMNTELGFGRRLLSIIESRGISFEHMPSG
                     IDSISLVISDCQLNEKLAGIIEDIEQQLKPDAVDVHSNMALIATVGQGMARTPGISGT
                     LFSALGGEDINVRMIAQGSGEMNIIIGVENEDFERAIQTIYYAFVN"
     gene            13612..15309
                     /locus_tag="VFD89_06775"
     CDS             13612..15309
                     /locus_tag="VFD89_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZJ57930.1"
                     /translation="MGKKVLIVGGVAGGASAAARIRRLDESAQIIMFERGPDVSFSNC
                     ALPYHLSGIIEDADSLVLMDPEDFVTKYNIEARTHSEVVEIKRAEKKIVVKDLVKGCE
                     YEESYDKLVLSPGARPILPKSIAGIDGPNVFSVRNVSDIRSLNEYISHNGITDIAVVG
                     GGFIGIEVAENLALAGKNITLIEALDQVMAPFDYEMAQLLHKEIHDNGIRLVLNDGLK
                     AVHDGNVELQSGKIIGAGAVVMAIGVSPEVDLARDAGLLIGETGGIKVDHNYLTNDRD
                     IYAVGDAIEVYNRLTHRMCRLALAGPAQRQARAAADHIYGIPHNNNGVIGSSIVRLFS
                     LNAASTGLNEKTAEAEGIPHDFVYIIANDKVGLMPDSSPMHFKLVYEYPTGRILGAQA
                     IGKGNVDKRIDIIATMILMGGTLEDLKELELCYAPLFGTARDIVNLAALVGLNILNGE
                     FRQVPVYKVRELVEKDAFIIDVREKYEYEAGHLINAVNIPLSELRDRLDEIPKDEPVY
                     LACRSAQRSYNAIMALQHLGYQNLYNISGSFLGICCYEYFMDMVTGREKIVTGYNFR"
     gene            15764..17236
                     /locus_tag="VFD89_06780"
     CDS             15764..17236
                     /locus_tag="VFD89_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HZJ57931.1"
                     /translation="MEILLRQLRDVSLSVLPITILVVILNYTIVPMDNQMFIRFLIGA
                     FLVILGLGIFLFGAHLGIAPIGNLMGETIAKTNKSYFVGSLGFLLGFLITIAEPDLQI
                     LAHQVNLASGGIVPSSLILIVVSVGVGIMVGIGLLRILYEKPLNKLFTLVYFIILILG
                     FKTSQEFLAISVDASGATTGAMTTPFILALGYGVSQLKGGKTSEEDSFGMVGLASAGP
                     IFAIMIMSIVMGLTDIQGEVGDLAVNLGILNPYLRTFPKVAREAVVTLIPLLLLFLIF
                     DKLRFKLNIRSRKSILKGLLYTFIGLTLFLVGVNAGFMEVGRVMGEGIARDYTWLLPI
                     IGFLLGMVVVLAEPAVHVLTEQVEDVTAGHIKRVFILIALSMGIAFAVSMSMLRIMIP
                     GLKLWHFLLPGFALGAFLSYRVPPIFVGIAYDSGGVASGPMTATFILAFAQGAAKAIP
                     SANVLIDGFGVIAMVAMAPLVAIQTLGLIFKIKSKKKESN"
     gene            17237..17902
                     /locus_tag="VFD89_06785"
     CDS             17237..17902
                     /locus_tag="VFD89_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZJ57932.1"
                     /translation="MDWLNKGLVLFWCILDFGKGSRLFRLSNEMGGIGATIFLGRGTI
                     KNELLNILGIMDTRKEIFFTVIDEELEQTFYERVTGKFSLDRPSHGIAFSMPIKYCSR
                     VDGSKNIRGPKKEGGYNLGYEAIFVVVDKGSIDDVLEASESAGSTGGTIIHGRGSGTK
                     EKETLFNIQIEPEKEIVLILSKAQKTESIVGAIKARLNLNEPGTGIIFVMEVGRTVGL
                     YEE"
     gene            18081..19490
                     /locus_tag="VFD89_06790"
     CDS             18081..19490
                     /locus_tag="VFD89_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HZJ57933.1"
                     /translation="MIEFGTGGWRAIIGDEFTKDNVCLLTQALADIINEEGHGSQGVV
                     VGYDRRFLSDKAARWITQVLAGNGINVLFIEEYAPTPLVMYAVNKYKTPYGTAVTASH
                     NAYDYNGIKVFTRGGRDADEGLTNKIEDQIARTDLIDVNSVEFLAGVNKGLIRTIDPF
                     NEYIDSILSMLDLNAISKMRLKVLFDPMFGASKISLNTVLNIMRCQVDIINAKHNPLF
                     GGRIPSPTSYTLKALSNLVPEQGYDLGLGTDGDGDRLGIVDERGEFVHPNDILCLLYY
                     YFLEYKGWKGGVVRNIATTNILDRIAEEYGQDCFEVPVGFKHISSAMDKHDAVIGGES
                     SGGLTIRGHIMGKDATIAAGLMIEMISVTGMSISQLMDGIGQRFGYTYFDERNIEFNG
                     GSRARISRLLFEDKRLPAFPYDIDRISYRDGIKIYFKNRGWAVLRFSGTEPLLRVFVE
                     MPTREDADKTLNIMEAFIQ"
     gene            complement(19587..20534)
                     /locus_tag="VFD89_06795"
     CDS             complement(19587..20534)
                     /locus_tag="VFD89_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZJ57934.1"
                     /translation="MRYRLSAYLALVIVFSIILGGCSGAKTLNIAIVLSEDSQDTNNN
                     FLQGAKAAAEEMDINLTYKITAKNTSISKQAMFIDSLKDDGIDALVIYPLEYHKIDSK
                     LKDLNIPVVVINSLPTSEINGDKNSYVYTDPVETGELMAESFLEKLNSGNQIAAILPN
                     VSNSIAIEQLNSMKAALGENGIHVVEVSLDSDDALTAHKQIATFIAEHTYIKAIAAFN
                     AIAVEGVAEAVVQQDVDMFMVSSDASIATINYLENGIVETAIAQSPFRVGYLGVQNAI
                     KMAGGDNVSRITSLRPQRVTLENLFDPDTENLIFPFQAP"
     gene            complement(20501..22246)
                     /locus_tag="VFD89_06800"
     CDS             complement(20501..22246)
                     /locus_tag="VFD89_06800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZJ57935.1"
                     /translation="MKILRAERSVSKLLYGSILFLFIIAIGLLFVLNYFLFANILNRG
                     YTSQLTTTTSQLKSNLELTMSSFRNTFNNVAVTILHADNLDSGDLNAKLSTIFSTNND
                     IVTIALFDYDGDLIYSQTENRLKPTVDAKEQSWFAEANTINKFTVPHVQNLFTGKYDW
                     VMSLSRRIAYKKDGAIHPAVLLMDIKFSSIAAVCSGAQSKSSGYVYIVSNEDLIYHTK
                     QQLVLMGLWKEDFQGVLTQSINHTYTSEINGKKYMNHVDYMPYENWYVVNAAYLGDLG
                     YTWRFYVTRLILISVFMLMLILPTSYLFTRRITLPLKDISEKIYGINDRHLDVTFDED
                     NSILEINMLAESINRLIVYIKRLLREQELKQIELRKAEFASLQTQITPHFLYNILGSI
                     IWMIEDGKKRGAIEMITALTDIFKLNASTTASTIPLSFELEYVDRYVHIQSLRYGNKF
                     EFYIECGPETEAFLCPRMILQPIIENAIEHGLSTLDSGKIFVKAYTHIGLLKISVKDN
                     GIGMTEDSLKELRRSLANTTGTLDSKPGSKAHGIALRNIHNRIQLLYGDGYGLRIESE
                     EDEGTCVIVCLPILR"
     gene            complement(22243..23844)
                     /locus_tag="VFD89_06805"
     CDS             complement(22243..23844)
                     /locus_tag="VFD89_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZJ57936.1"
                     /translation="MYKLVLVEDEIETLENIVRNIDWETCGFTLAGKALSGQEALSIV
                     DQVKPDLIITDIRMPYMDGFEMIKRVHEKDNSICFLIISGHDEFDYAQLALQYGVLDF
                     IIKPVTKEIIEKSLGKAKAHLDKQYAIRNDMDTLRQQLKESMDLLRSSFLIEILSKGV
                     SDEYFNMAVGSYDLPQRSTFSIIAIQTESTGKGRKLIDRHFSGKQEIMWLALRNLVEE
                     QINADFTSYILQYQERLVIYAFSHKRNLIALCDRIVAAAEKLWGLKLIVGISNPCSSL
                     RNANVYYLQAVRALNLDFVNNEQRVYCYSDLQLEQDESPTLLDLTLDESVIINGEKDD
                     IAGVFDNIAQSLAEAQLSEQRLSSTQAEINSQLLRLADDFDVDVLSKFPVHQIYDHFQ
                     DINGWIQLITLIAEYINREIQDKNYCTGTLLMAHAKSYIDENYTDPELSLDRICDHLD
                     ISASYFSALFKRDFSQTFLQYLTSLRIQKACSLLLNTDKKSSQIAREVGFTSPNYFSY
                     RFRQYVKTSPVEYRRKGREREKQSQ"
BASE COUNT         6723 a         4892 c         6155 g         6376 t
ORIGIN      
        1 ccatataaat atatcaataa aattatttta gcatgaggag gtaattggtg tatgaaaaag
       61 gcgaagatga tacttgataa ggattttaaa gtaggtcagg tggatgatag gatctacggc
      121 tccttcatcg agcacctagg acgcgccatc tatgggggaa tctatgagcc ggagcatccc
      181 gaggcagatg aaaagggctt tcgaaaggat gtcactaggc ttgtaaagga acttaagatg
      241 ccaatcgtcc gttacccggg ggggaacttt gtgtcaggat ataactggga agacggggta
      301 gggcctaaag agaatcgccc aaggcggttg gagctggcct ggaggaccat cgagaccaat
      361 caatttggca ccaacgagtt tgtggattgg gcaaggcagg cggataccga ggtgatgatg
      421 gccataaacc ttggcacacg gggcgtagac gccgcacgca actttgtaga atattgcaat
      481 catccggggg ggacctattg gagcgatctc agaagatccc acggatatga aaagccccat
      541 aacataaaga cctggtgcct tggcaatgag atggacgggc catggcaaat cgggcacaag
      601 actgccgacg aatatgggcg gattgctctg gaggcagcca aggtgatgaa atgggtggat
      661 cccactattg aactggtagc ctgtgggagc tctaatatag agatgcccac ctttgcccag
      721 tgggaggcga cggtactgga ccatacatat gaactcgtag actatatctc cctgcatcag
      781 tactatggga accgagacga tgatacgcct aactttttgg ccaggaccat ggatatggat
      841 ctgtttataa agtccgtagt ctccatatgc gattatatga aggccaaaaa gcggagtaaa
      901 aagaccatca acctatcctt cgatgaatgg aatgtatggt ttcattccaa cgaagccgat
      961 aaaaaaatcg agccttggac agtagcaccg ccccaattgg aggacgtcta tacctttgag
     1021 gatgctctgc tggtaggttc catgttaatt accatgctta ggcattgtga tagggtaaag
     1081 atggcctgta tggcacagct ggtaaacgtt attgcgccta ttatgacgga agatggcggg
     1141 gcagcatggc gccaaaccat cttttatcca tacatgcatg catccctata cggaagggga
     1201 gttgtccttg atccggtgat ttcctcaccc gtatacgata ccaaagaata ctccgatgta
     1261 ccctgtttag attctacggc ggtattcaat gaagaggaag aagagctgac catattcgct
     1321 gtaaataggg gtatggagga ctctttagtc ttggaatgcg accttagaaa cttcccggga
     1381 tatagggttt tagagcatat taccatggag catgacgatg ttaaagccac aaataccagg
     1441 gataatccat ataacgttgt cccggccgat aggcaggata cagaggttgc agacggacaa
     1501 ctgacaacaa ggctcaaaaa cctttcctgg aacgtaataa ggcttggaaa aagcgggcaa
     1561 taggaaggat aaggagcttg ccggatggaa gatgacaagg aggatttgac tttggttggg
     1621 aaaaaacaaa tgctgagaaa tataaaatac ttcattttgg atatggacgg aaccttttat
     1681 ttaggggata agctattgga tggctctttg gaatttatag ataccttgga gaagacaggg
     1741 catgattttc tcttctttac caataattca tccaaaaacc ctgacgtata tatagacaga
     1801 ctaaagggga tgggatgcac cgtagagaac aacaaggtag ttacctccgg gatggttacg
     1861 attcatcacc taaaaaaaca tcacaagggg gccagggtct atttattggg cactccggag
     1921 cttgaggagc agttcattaa tgaggggata gaactggtag atagcaatcc ggatttggtg
     1981 gtagcgggat ttgacaccac tataacctat gataagctga gccgcgcctg tacctctata
     2041 aggcaggggg cgacctttat agctacccac ccggacttta actgtcctac ggaagacggg
     2101 tttatccccg actgcggagc catctgtgca ttcattacgg cgtctacggg ggttaagcca
     2161 aaatacttgg gtaaacccta taccgaaacc ctagagttta tcttagatca cctaaattgt
     2221 acggctgatg atatcgcctt cgtcggggac aggctttaca cggatatagc cataggggta
     2281 aaccatgggg taaccggcat tcttgtaatg accggtgagg ccacagaaga ggatattaaa
     2341 aaatccgaaa tacaaccgga tattatagtg gaaagacttg tagatattac caagtggcta
     2401 taaaatggtt ataggcaacc tattgtgcta attaaatata tcggtaatga ctattgaacg
     2461 ctacctccaa cattgatttg gagaaaacta aggcttattc catttgaaaa gcctatcgct
     2521 tccaatccgg cctcctgccg gtggaatcgg cttaaggcgt cctgccttaa gctacggctt
     2581 ttctgcatta cataagccaa gttttcttgg cccaatcaat gaagtcatcc gcttttcaat
     2641 agcccattac ctttataatg cactaagata atggactatc acaatgcgat aaataagaaa
     2701 acgaagggga gataatgatg gcatttattc aatgtgattt cttttcggag gtgttgggtt
     2761 tatccacctc catgaacgtg attttgcccc agcccgcctt gggccaaatc ggtatggaag
     2821 gcagcaaggc ttcggataag tatcctgttt tgtatctact ccatggattg tccgatgatc
     2881 ataccatatg ggggcgccgt acttctgttg aacgctatgt ggcatcttta ggtctcgctg
     2941 tagtaatgcc cgccgtacat aagagctttt acacagatat ggccaaaggg gacaaatatt
     3001 ggacctttgt aagcgaggaa ttgcctcaga tagtgcgatc ctttttcccc atatccgcag
     3061 ccagggagga taactttgtg gccggcctat ccatgggcgg gtacggggcg tttaaattag
     3121 cccttcgttg ccctgataag ttcatagcag gggcaagcct ttccggtgca gttaatattg
     3181 ccgaaatagc caaaaacaga gacccgggaa gagcttggga gaacgtattt gggaatttag
     3241 atgagatcaa gggaagcgat aatgacctgc ttcacttagc agaagaggtc tcaaaatcca
     3301 atggtccaaa gcccatgctc tatcaatgct gtggcactga agatttccta tatgaagaca
     3361 atatcgtttt tagggattgg gctaatgagt tggggctgaa tctgacttat gaagaggaac
     3421 ccgggaccca tgaatggggt tattgggata aaaagatcca aagggtgctg gagtggttgc
     3481 ccatgaataa ttccaaatag gattggacaa ataagattcg acaaaacaaa gagccgcgga
     3541 ataaattcgc ggctctttat gtaaggggtt tgggggtttg ggtaacttac gttattatag
     3601 taacccattt tatgaaaaat aaacattaaa aataaaaaaa gttttgggtt tatcgaacct
     3661 gttgataatt agcggataaa tatgtactat ataagggagg gtaccggata atagggaatc
     3721 atattacaaa ggcgcaatga taattatttt tgcgataaaa attattttat ggataaacaa
     3781 tttggaatat aaaatcttcc attatgaaat tgggggcccg ttacatatac caatcatacc
     3841 gacagattta gttgatccat tgtttatcta cagatacagc cggaaggagc gaattataaa
     3901 tggaagagtt aaacaaaaag ctaaagatgc gaaacctccc ccacgtgttg gtggaccaag
     3961 aaggcaagat catcaacgac gacgaggcgt gggcaagccg caggcaagag attgttaaat
     4021 tatttggaag tcaagtgtat ggattttcac cgcccgcacc attaaaggtc tcttcgaaat
     4081 tggtgaggga ggatgaggac gcattcgcgg gtaaggcagt ccactccatt atagacttaa
     4141 gctttgacac tcctgggggt gaattttcct tccctgttaa cttggttata cccaagggtg
     4201 tgtcaaaggc gcccctgttt ttacatatcg cttttagccc ggatgttccc gacagatact
     4261 ttccggttga agagatcata gacggcggat ttgcggttgc ctccttttat tatgaggata
     4321 ttaccagaga tgaggacgat gattttaaat ccggtctggc gggaatgtat agtggggaga
     4381 atcgcaaacc cgatgaatgg ggaaaaatat ccatgtgggc atggtccgcc caaagggtta
     4441 tggattttat tcagaccgtc gaagcaatag ataaaggcaa agtagccgta gtcgggcatt
     4501 ccaggctggg gaagacagcc ctatggtgtg ccgcccagga cgaacgcttt ataatgggca
     4561 tatccaacga atcaggttgc tccggcgcag ctatcagtcg gggcaaaaat ggggaaagga
     4621 taaaggatat tgtcaaaacc ttcccctatt ggttttgtga taactacaag gattattctg
     4681 ataatgaaga gaatatgccc tttgatcagc actttttaat ctcggccata gcacccagat
     4741 atgtttatgt gagcagcgcc gttggcgacg agtgggccga tccggaatca gagtttttag
     4801 gctgtgttgc agccggggat gtctacgaat tatttggggt taaaggcctg gtcactgagg
     4861 atgtgctccc gaaggtaggg acaaaattgc atgacggaca tattgggtac cacctaaggg
     4921 gaggcaccca ttatctcagc cgttatgact ggcagggctt tatggagttt atgaagcgaa
     4981 aactgtaata attcaccctg ctgatctagt tgaatatagc ggtaatggct attaaacgct
     5041 tctttcatca ctgattcgga gaaaattagg gcttattcca tttgaaaagc ctatcgcccc
     5101 caatccggcc tcctgccggt ggaatcgact taagacgtcc tgtcttgagc tacggctttt
     5161 cttcatttca taagccaagt tttcttggcc gaatcagcga agtcatccgc ttatcaatag
     5221 tccattacct ttagggcaca gtaaaaaaat ggctagcaca atgggtcaat caaaacttta
     5281 gatatatggg aggagaaaaa tgaaacagat tgagaaaaac agcataaatg ccatacgcgt
     5341 tctttcggca gaaatggtag agagggcaaa atccggacat cccgggatgc ctatgggagc
     5401 ggccccgacg gcgtattccc tttgggctag gcagatgaag catagcccaa ataacccgga
     5461 ttggctggat agggatcgat tcgtgttgtc tgcaggtcat gcgtcggcac ttatctattc
     5521 cctgctccac ctatttgaat acggactttc catggaggat ttaaagaatt ttcgtcaatg
     5581 ggacagcaag acgccgggac acccggagta cggtcatacg gttggggtag agactaccac
     5641 cggccccttg ggacagggct ttgccaccgg tgtgggtatg gctctggcag aagcccatct
     5701 ggctgccaag ttcaacaggc cgggatatga gatagtggac cattatacat atatcctatc
     5761 cggtgacggt tgcatgatgg aaggtataac ctcagaggct gcttctctgg cgggtaccct
     5821 agagctagac aagctcattg tactctatga ctccaacgcc atcaccatcg aaggcggcac
     5881 cgatatggcg tttaaggagg atgtgggcaa gcgttttgag gcctataact ggcatgttct
     5941 aagggtggaa gacgggaacg atgtagatgc tatcgcgagc gcaatagagg acgcaaaggc
     6001 ggaagtgaaa aaaccttccc tgattattgt aaccactcag ataggatacg gttcccccgc
     6061 aaagcagggt aaggcttctg cccatggcga acctctgggt gccgagaacc tcaaagccgc
     6121 aaaggaatat ttgaaatggc cctatgagga agaattcttt gtaccccagg atgtaaggga
     6181 acatatggca tccattaggg ataagggcgc agagaaagaa aaggcttggc aagaaaaatg
     6241 ggatgcctat tcaaaagaat atccggaatt ggccagggaa tgggatctat ggcatagcaa
     6301 ggatttgccc atagacctat taaacgatga ggaattttgg agttttgatg gtaaggatgc
     6361 cacccgctca tcctcaggaa aggttattaa ccgcttgacc aagatattgc ccaatctaat
     6421 cggcggctct gcggatctgg gtccttccaa caagaccttt atggaaggca gggacagttt
     6481 ctcggcagaa aactatgggg gatccaacct gcactttgga gtccgcgagc atgctatggc
     6541 agctatggga aatgggatgg tgctccatgg tgggctgagg gcatatgtcg ccaccttctt
     6601 tgtatttacg gactatatga aaccggccat gagattgtcc gccctaatgg aactgcccct
     6661 catatacgtg ttgacccatg acagcatagg ggttggggag gacggaccta cccatgagcc
     6721 tatagagcat ctggcggctc tgaggagtat accgggcttt accgaatttc gtcccgccga
     6781 ctccaaggaa accgcggccg ggtggtacgc agcacttacc agaggggaca gtcctacggg
     6841 cctaatcctc agtcgtcaaa acttacctct gtacaaggaa accggcaaag aggccctgaa
     6901 gggcggctat atcctgttgg attcagaaaa ggagatccct gatatcattt taatggcctc
     6961 cggttcggaa gttgagctta tctacgaggc ccatgccgta ttacaggaac aaggaataca
     7021 tgcaagggtt gtaagcatgc cttccttcaa aatatttgag gagcaatccc ccgaatacag
     7081 ggaaggggtg ctgccgaata tggtaagggc acgtctggca gtagaggcgg gctcatcttt
     7141 cggatggcat aagtacgttg gtcttgatgg ggatataatt gccctggacc gctttggtgc
     7201 ctccgcaccg ggtgaaattt tattcgagga gttcggattc acagtggaca acgtcgtaag
     7261 cagggcaaaa gcccttatag ggtaaactgc ttatatcaca gggctatgta ctgattaaaa
     7321 tttccaccat ggcgattgca gattttcagc gcaatctcat ggtggaattt ttgtaatagg
     7381 ctggctataa gttcggcctt aatcgtccaa tatctccaat tccaaggcat caaaatcgat
     7441 gggctcaatg aaattatcct gggttaacaa tattctcctg taatcctcgt actccctttg
     7501 attttttggg agccacatag gccgttgaag gtcaaagtcg tagcatatcc tttgaataca
     7561 atggtggagc atatcaattt cccttatatc atcccccgca taatctgcaa ccatgtcctt
     7621 taggattttg ttattttttc tgattattcc ccatactcta agcatatcgc agttctcctc
     7681 acataataat aaccttagtt ttccccaagt caatgtcgaa agtaaggaag tttcataaaa
     7741 acattaaccg gcacaatcgg tcaaattaat tgtttctcat tatagcagac aataggtccc
     7801 aaggggaagg gaaaactttg gtttagcccc ctagggatag ggaaagatag gggtaaggca
     7861 ggaggtataa agcttgatcg gaaggttctt aaattttatt ggctgcatgt atcgcagatt
     7921 tttagacgat gatattctcg ctctgggagc tcaaatggcg tattatttgg tgctgtcctt
     7981 tttccccttt ttgatatttc tcatatccct cataggctat agccccatct ccagtaggca
     8041 ggtattggag gggctgctgg gcatcctgcc ggaggaggcc tatatcctgg tcagggatac
     8101 catcgtagag attaggggga caagaagcgg ggggctggtt tctgtaagcg gggtgttgtc
     8161 cttatggttc gcttcgaacg gaatcggatc cattataacg gggttgaata aggcctatga
     8221 cgaaagggag aagagatcct ttatcaaggt aaaactgatc tccgtctttt ttaccctcct
     8281 cctttcggta gctatcgtat tttcgtttgc ccttttggtt tttggtggcg ttattaagag
     8341 acgattgata gactgtataa gccttggacc cttccaccgg ggtttatggg acgtgggacg
     8401 ctacgtattt atctcgtccg ttatactgct gatatttata atattatata gatacacccc
     8461 cagcaggcgt atgggatggc gggaggttgt tcctggggca atattttcaa ccgccggctg
     8521 gcttgggacc tctacaggtt tcgcctatta tgtggataat tttggccatt actcaacatt
     8581 ctatggcggt ataggcggcg tggttatcct ccttatttgg ctgtatttca gcgctgtcat
     8641 aatactgatt ggaggggagc tgaacgcggc cttggtgtat aataaggcga agtgatgcca
     8701 gtgggcacta ttaagggaca tgccgatgtc cgatgacaag catatcggtc taaggtgcgc
     8761 ttgcccttta atatcatcgg tgtgctacaa ttgctgcgac aatcgccaca ataacccttt
     8821 ggaacttaaa tgattggaat actattctta gaatttagat tatacaccct atggggagat
     8881 gtcttttgac agtagaacaa tattttggta aaattcttgt ggtcgggacg ggtttgggtc
     8941 ttaaaatgaa tgagaaacgg ggtgtgctat gtgaacatct atattatggt ggatgcagaa
     9001 ggtatcagcg gcgtatataa cagcgatcag gtgatgtcaa cggggagcag gtatcaagag
     9061 ggcagggaac tcatggtacg cgatatcaac gcctgcgtag agggatgcaa agaggcgggt
     9121 gcagatagga tatatgtaag ggattgtcac ggcagcggat caaatgttgt atggagccga
     9181 ctttcggacc tggcagatta ctacataatt ggggataccg gccaggatcg ttttccgggt
     9241 cttgatgaat gccatggggt aatactcctg ggatatcacg ctatggcggg cacgcccggt
     9301 gctatcttag agcataccat gagctcggaa gcggtacaga actattggat taatggggta
     9361 aaggtcggtg aattggcgat agatgccggt atagtggggg acaggggaaa acccgtaatt
     9421 atggtctccg gagatgataa ggtgtgcctg gaagccaaag gattgcttcc cgatgtgatc
     9481 accgcccaag ttaagaaggg catcacatgg aggggtggga tgcttcttcc tccgcagaag
     9541 tcctacaatc tcataaagga aaaggctaag gaggccgtta acctgctaaa tatgcagcag
     9601 gcgctggtat tttctaagcc cataaggctg agagtagaga tgaccgaaag gggaatactt
     9661 cccctgcaat attccaaacc ctatatgact gttatcgacg gtaggacata cgaagtcgag
     9721 ggcagtaccg tagaggaggc cctttttaga ttataacgag tcggcaaaat atcccccccg
     9781 cttctatcaa gtggcaagct cccattagta aaataggcgt tgggtataaa tcatcctccg
     9841 cctcgttgaa acctgtgtgt tgcaattgcc aatgcaattg cttccgatgt tataacgagt
     9901 tgggtatagc ctggaacctt ggcagctgaa ataacgttaa gaaaggattg aacctatatg
     9961 aaaaacatag tgaatctcga aagctttgcg ctggagaacg gtgaggcctt tgttttggac
    10021 agggagcatg ccctaatcaa gctgaataag gggcagggaa gactcgtcta tgaaggcgag
    10081 cctatgaacc tgggagaagc cgattggaag gatgcaaagt atctggtttt cgatgttaaa
    10141 ggatatgagg attgggatat tccgctcctc atagaatttt ggaatcccga caataaaaca
    10201 gaaacaccca ccctgcatta tatcgtgggt cttttgccta agatccgcac cagggtatcc
    10261 cttcccttgg aagccttaag ctctgaggaa atgtttttga ccaggacccc ggttaagctt
    10321 aagaccgtaa ttcatggcga cagggtatac ctagaagagg ccaacaggtt tgccataagc
    10381 actaggccat cgtcatttga gcagagactg gagataacca acatgtacct gacggacaaa
    10441 gagccggact atcctgttcc cgatgtagtc ctggtagacg aattaggcca gtatacaaag
    10501 agggaatggc cgggtaaggt tcatagtgag gacgaaatgg tgaactatct tcatagggaa
    10561 ttggaaactc ggaacaaaaa cgatttgtcc gaattttccg atgagtggag tatatatggt
    10621 ggttggaagg gcaagcaatt ctcatccacg ggatatttta ggacggaata tgacggcaaa
    10681 agatggtggc tggtggatcc cgaagggtac gccttctaca gcaacggtct tgattgcata
    10741 cacccccacg aacagttctc ccatatctcc ggaatcgaaa agttcgcatc atggcttccc
    10801 gaaaaggacg gtaaatttag ggacgcttgg cgcatgtcag agggaaggtg ggaagcctcc
    10861 ggtgaaggct cggaagaata tgattttgct atatccaatt tgatacgtgc ctttggggat
    10921 aaatggttcg acagctgggc cggaatgacc aaaggaagga tgatcgactg gggctttaat
    10981 accgttgcca attggtccga tgccgggttc atacgctttg cgaaaatgcc ttatgtttgg
    11041 cctatgaggg attttcccga gacagaaaaa aagatattca gggatttccc cgatgtattc
    11101 agtgatgaat ataaagtgaa cgccttaaga tatgcacaac agctgaggga gtttgaaggc
    11161 gatagaaata tgatcgggta ttttctgagg aatgagccga tatgggcctt tgtcgatatg
    11221 aacatcgccg aaaagctcct tgaaaatgag catatgacgg atacaaaggc ggaacttatt
    11281 aaaacccttt cagataagta cagcggtgat atcgccgggt ttaatgctgc ttggaatctt
    11341 gatttggata gtttcggcga attgaaaaac agtatcaggg gcgctacaag gttatccccc
    11401 agagctttgg aagacttgga agagttctcc cgggtcatga tcgaacgcta tgtaaaaata
    11461 cccagtgatg cagtaaagag ggtagatcca caccatctaa acctgggcat gaggtatggg
    11521 actttatcca gagataatct agcggccgga tgcgaaaatt ttgacgtttt ctccatcaat
    11581 tgctatagca tgaacccgaa ggacgacctc atcaaggctg ggacccttac gggaatgccg
    11641 gtaatgatag gcgaatatca ctttggtgcc ttggaccggg ggcttatggc cacggggcta
    11701 agggcggtat cgtcccaatc ggagagggga aaagcctata ggtattattg cgaaaatgca
    11761 gccgctacga agtattgtgt gggaacgcat tattttaccc tgggagatca gtcctttttg
    11821 ggacgagggg acggggaaaa ctaccagatt ggaatagtca atgtatgcaa taggccttat
    11881 gaagaaatca tagggggcat gattgagacc cataagcgga tttatgaggt tgcggagggc
    11941 acaagggaag cgtataatac acgccctgac gaaattggat ttattgctta ttgacaattt
    12001 gttctctgat aatataatgg ggcagaagat gtcttgcgtt tctataagcg gtggatttcg
    12061 gctaattaaa atggatagcg gacgtatgtt tcccgcccca ttgaaacctg tgtgttgcaa
    12121 ttgcattgca attgcttccg gtgttacaat agttacagtc caaaagatta tagataaaca
    12181 accctgagat aaaatttatc atctacagat aagtttggat ttcgtattgt ttatctgcaa
    12241 atgagattat acatgtatgg aggtttttta tatatgggta tcaaggttgc caaattcggg
    12301 ggttcttccc tggctgatgc agatcaattt aggcaggtgc gctccatcgt catgatggac
    12361 gaggggcgca ggtacgtaat tccgtctgcc ccggggaaaa ggcattccgg cgatcacaag
    12421 atcacggatc tattatacct ctgccaagag catgcccaga agggggttcc cctcggagaa
    12481 atttttgaat tgatttccac aagatatcta cagattgtga aggaattgga gttgttcctg
    12541 gatcttaaac cgatccttga agatatcaag gacaaggtac tgggcggata ttctgttgag
    12601 tacaccgcca gcaggggaga atacctaaac gggctgatac tggccgaatt tttggggtat
    12661 gaatttttag atcccgcaga tataatattt tttacccaat ctgggatata tgacgaacaa
    12721 cgaacccagg aggcggtaga gaaggccctt gctgatattc caagagcggt tatcccaggc
    12781 ttttacggtt caagaccgga cgggggtatc caaaccttct caaggggcgg ttctgatatc
    12841 acaggcgcta ttatagccag ggggatcaaa gccgatcttt acgagaactg gaccgatgtt
    12901 tccggattta tgatggcgga tcccaagatt gtaaaaaacc ccagaccaat agaggagata
    12961 acatataggg aattgaggga actggcatat atgggggcaa aggtgctcca tgaagatgcg
    13021 gtcttcccgg tgagacaagc taggatcccc atcaatataa agaacaccaa cgaaccggag
    13081 gctgtgggaa ccctgatcgt accgaacggc ggagaatttg ccagggccgg tatgataacc
    13141 gggatagcag gcaataagga ttttacggtt atcaccgttg aaaaggctat gatgaatacc
    13201 gaattgggat tcggtcgcag gctcctgtcc ataatagaat ctcggggcat atcctttgag
    13261 catatgccat cggggatcga cagtatttcg ttggttatat ccgattgtca actgaacgaa
    13321 aagctggcgg gtataattga ggatatagag cagcaattaa agcccgacgc tgtggacgta
    13381 cattccaata tggcccttat tgctacagtc ggacagggca tggccaggac cccgggaatc
    13441 tccggaacac ttttttctgc gttgggcggt gaggacatta atgtcagaat gattgcccaa
    13501 gggtccggag aaatgaacat cataattggg gtagaaaatg aggacttcga aagggcgata
    13561 cagaccatat actacgcttt tgttaattag taaaagaagg aggatattgc catgggcaag
    13621 aaggttttga tcgtaggcgg agtggccggc ggtgcctcag ctgcggccag gataagaagg
    13681 cttgacgaat cagcacagat tattatgttt gaaaggggac cggatgtctc cttctccaat
    13741 tgtgccctgc cctatcacct aagcgggata atcgaggatg ccgacagcct ggtgttgatg
    13801 gatcccgaag attttgtaac taaatataat atcgaggccc ggacccacag cgaagttgtc
    13861 gagataaaaa gggcagaaaa gaagatcgtg gtaaaggatc ttgtaaaggg ttgcgaatat
    13921 gaagaatcct atgacaaact ggtattgtcc cccggtgcca gaccgatact cccaaaaagc
    13981 attgcgggaa tagacgggcc caacgtattt agcgtaagga atgtgtcgga tatcagaagc
    14041 ctcaacgaat atattagcca taacggcatt accgatatcg ccgttgtcgg gggcggcttt
    14101 atcggaatag aagtcgcgga gaatctggct ttggcgggga agaatatcac cttgattgag
    14161 gcccttgacc aggtgatggc accttttgat tatgagatgg cgcagctgtt acataaggaa
    14221 attcacgata acggtattcg gctggttcta aatgacggat taaaggcggt tcatgatggt
    14281 aacgtagaat tgcagtcggg taagataata ggggcagggg ccgtggttat ggcaatcggg
    14341 gtcagtcccg aagtcgatct ggccagggat gccggcctgt taataggcga aaccggcggc
    14401 attaaggtag accataatta cctgaccaac gacagggata tatatgccgt aggtgatgcc
    14461 atcgaagtgt ataatcgcct aacccatagg atgtgcagat tggccttggc tgggcctgcc
    14521 cagcgtcagg caagggcggc cgcagaccat atctatggga tcccccataa taacaacggt
    14581 gtaataggtt catccatagt ccggttgttt agcctgaatg cggcatctac aggattgaat
    14641 gaaaagacgg ccgaggcaga ggggattccc catgactttg tctacataat cgccaacgat
    14701 aaggtgggcc tgatgccgga ttccagcccc atgcatttta aattggtcta cgagtatccc
    14761 accggaagga tattgggtgc ccaggccata ggaaagggca atgtggataa aaggatagat
    14821 ataatagcaa ccatgatcct catgggcggt accttggagg acctcaagga actagaactt
    14881 tgctacgcgc ctttatttgg gacggctagg gatatagtaa atctggctgc cttggtaggg
    14941 cttaacattc taaacgggga gtttaggcag gtgcctgtat acaaggtaag ggagctggta
    15001 gagaaggacg cctttatcat agacgtaagg gaaaaatatg aatatgaggc agggcacctt
    15061 ataaatgcgg tcaatattcc cttgagcgag ctaagggaca ggctggatga gatccccaag
    15121 gatgaacccg tatacttggc ctgcagatca gcccagagaa gctataacgc gattatggcc
    15181 ctacagcatt tggggtacca aaacctctat aatatctccg gttcctttct gggaatctgt
    15241 tgttacgaat actttatgga tatggtgacc ggaagagaaa agatagtaac cggctataat
    15301 ttcaggtaag actatcaaaa ttgggccgta aaccatacat actgttaggt ttacggtctt
    15361 ttattgtctt caatccggga aaagttatag caagtatatt gaaactctgg tagaataaag
    15421 ttgggaaaat gaaggccctg tggctatcat atatagttga ttaatgcatt gaaataaatt
    15481 aacgcgttat gctatatgtt ttacgacaaa cattctacat tggtacatag gtgtcgtaaa
    15541 acattatcct taacccgtat gttacgaaaa cacattgttt taaggtaaaa tcggataatt
    15601 ttgccgattt tacattgaaa gatctttttc gtaacatata tagttcgtca acttactgtg
    15661 tgataaaggt aattagctat tgaaaagcgg acgacttcat cgattgctta cctatagcta
    15721 gcgcaataga ttaaatcatt acacataaga aaggggaagt tatttggaaa tactgttaag
    15781 gcaattgagg gatgtatctc tatccgtttt acctataaca attttggttg taatcctaaa
    15841 ctatacaata gtgcccatgg ataatcagat gtttattaga tttttaatag gtgcattttt
    15901 ggtgatcctg ggacttggga tattcttatt tggtgcccac ctgggtatag cccccatagg
    15961 caatctcatg ggggagacca tagcaaagac aaataagtcc tattttgtgg gcagcctcgg
    16021 ctttctattg gggtttctta ttacgatcgc ggaacccgac ctacaaattc ttgcccatca
    16081 ggttaatttg gcatccggcg gtattgtacc cagctcttta atcttgattg tggtctcagt
    16141 cggggttgga ataatggtag gcattgggct tctgaggatt ctatacgaaa agcccttgaa
    16201 caagttgttt accctggtct attttataat attaatactc ggctttaaaa catcccagga
    16261 atttctggct atatccgtcg atgcttcggg tgctaccacc ggggctatga caaccccctt
    16321 tatattagcc ttaggctacg gtgtatcaca attaaagggc ggaaagacat cggaggagga
    16381 tagctttgga atggtgggtc tggcctccgc aggtccgatc tttgcgatta tgataatgag
    16441 catagttatg ggccttaccg atatccaggg tgaggtgggg gatttggcag ttaatttagg
    16501 gattttgaac ccatacttgc gcacctttcc taaggtagcc agggaagcag ttgttacgct
    16561 aatacccttg ttgctcctgt ttctgatctt tgataagctt aggtttaaat taaatataag
    16621 gagcaggaaa tccatcctca aaggactcct atatacgttt atagggctta ccctgttttt
    16681 agtcggggtg aatgccggat tcatggaggt cggaagggta atgggggagg gtatagcaag
    16741 ggattatacc tggcttctgc ccataatcgg gttcttgcta ggcatggtgg tggtcttggc
    16801 ggagccggct gtacacgtat tgacggagca ggtggaggat gtgaccgcgg gccatattaa
    16861 aagggtattt atcctgatag cattgtccat gggtatcgcc ttcgcagtct ctatgtctat
    16921 gctgaggata atgataccgg gattaaagct atggcacttc ctattgccgg gattcgccct
    16981 cggggcattt ctatcctata gggtaccccc tatatttgta ggcatagcct atgactcggg
    17041 aggcgttgca tccggtccga tgaccgcaac ctttatcctg gcttttgccc agggggcagc
    17101 caaagcaata ccatctgcaa acgttttaat tgatggattt ggcgttattg ccatggtagc
    17161 catggctcct ctggtggcta tccaaaccct cggacttata tttaaaatta aatcaaaaaa
    17221 aaaggagagt aattagatgg actggctaaa caagggtctc gtcctattct ggtgtattct
    17281 ggacttcggg aagggaagca gactatttag gttatctaat gaaatggggg gtataggcgc
    17341 taccatattc ctgggcagag gtacgataaa aaacgaatta ttgaacatat tgggaattat
    17401 ggataccaga aaggaaatct tttttacggt tatagacgaa gagttggaac aaacctttta
    17461 tgaaagggta actgggaaat ttagtttaga cagacctagt catggcatag ccttttccat
    17521 gccgataaaa tattgctcca gggttgacgg ctccaaaaac atcaggggcc ctaaaaagga
    17581 aggtggatat aatttgggtt acgaagctat ctttgtcgtt gtagataagg gttcgataga
    17641 tgatgtctta gaggcgtccg aatcagccgg ctcgacaggt gggaccataa tacacgggag
    17701 gggatccggg actaaggaaa aggaaacatt gttcaatatc caaatcgaac cggagaagga
    17761 aatagtcctt atcctgtcaa aggctcaaaa aaccgaatcc attgtagggg cgattaaggc
    17821 gagattaaat cttaatgaac cggggacagg aatcatattt gtaatggagg taggcagaac
    17881 cgtcgggctg tatgaagaat aaaaggattg cgaaacctat attaccgagg tcgaagtcgg
    17941 cggataatta agacaagaac ttaaaagggg ggatcttccc ccttccggga acatcaaaca
    18001 ggagcctaag ggggaaggac cggaccagtg acgagtcttt taatatgctt taatgtgcct
    18061 tataaagggg gaaggaaaag atgatagagt ttgggacagg cggttggagg gctattatag
    18121 gtgacgagtt tacaaaggat aatgtttgtc ttttaaccca ggccttggcg gatataatca
    18181 atgaggaagg gcatggcagc caaggtgttg tggttggata tgacagaagg tttttgtcgg
    18241 acaaggctgc ccggtggata acacaggtgc tggcaggcaa cggaataaat gtgcttttta
    18301 ttgaagaata tgcccctacc cctttggtga tgtatgctgt caacaaatat aaaactccct
    18361 acggtacggc tgtgaccgca agccataatg catacgacta taacggaata aaggtattta
    18421 cccgcggggg tcgggatgcc gatgagggat tgacgaataa aattgaggat cagatagcaa
    18481 ggaccgactt aatcgatgtc aattctgtag agtttttagc cggcgtcaat aagggtctga
    18541 taaggacaat agatcccttt aatgagtaca tagacagtat tttgtccatg ctagatttaa
    18601 atgccatatc aaaaatgcgc ctaaaggtac tctttgatcc catgttcggg gcatctaaga
    18661 taagtctcaa cacggtattg aatattatgc gctgtcaggt ggacatcatc aatgccaagc
    18721 acaatccgct ttttggaggt aggataccct cccccacctc atatacccta aaggccctgt
    18781 ccaacctagt gccggaacag ggttatgacc ttggtctggg taccgatggc gacggtgatc
    18841 gattgggcat agtagacgag cggggggaat ttgttcatcc taacgatata ctctgccttc
    18901 tgtattacta ttttttagaa tacaaggggt ggaaaggcgg cgttgtgagg aatatagcca
    18961 caactaatat tctggaccgt atagccgaag aatatggaca ggactgcttt gaggtgccgg
    19021 tggggtttaa acacatcagt tcagctatgg acaagcatga tgccgtaata ggcggtgaaa
    19081 gcagcggggg tcttaccata aggggccata taatgggcaa ggatgcaact atagcagccg
    19141 ggcttatgat tgaaatgata agcgtgacag ggatgtccat ctcccagcta atggatggga
    19201 taggccaaag atttggctat acctattttg acgaacgaaa tatagagttc aacgggggaa
    19261 gcagggccag gatctccagg ctattgtttg aagacaaaag gctgcccgcc tttccctatg
    19321 atattgatag aatcagctac agggatggga taaagatcta ttttaaaaat cggggctggg
    19381 cagtgctcag gttttccggg acggagcccc ttttaagggt atttgttgag atgcccacaa
    19441 gggaggatgc ggacaaaacc ttaaacataa tggaggcctt cattcaataa catgcaacct
    19501 cttatattat gatcatttac aggaagatat tatagcgcag aatggctatc gcagaacata
    19561 tggttagaga aatacgggtt ctatccctaa ggcgcctgga atgggaaaat tagattttca
    19621 gtatccggat caaagaggtt ttccaacgtt acacgttggg gtcttaggga cgttatacgt
    19681 gaaacattgt ccccccctgc cattttaata gcattttgta ctcccagata tcctacacgg
    19741 aaaggggatt gggcaatggc tgtttcaacg atgccgtttt caaggtagtt gatagtagct
    19801 atggatgcat cggaagatac catgaacatg tctacatcct gctgaacaac ggcctcagcc
    19861 acaccctcaa cggcaattgc attaaaggcg gcaatggctt taatatatgt atgctctgca
    19921 ataaaggtcg caatttgctt atgggcggtc agggcatcgt cgctatctag ggaaacctct
    19981 actacatgaa tcccattttc ccccaaggcg gccttcatgg agttcaattg ttctatggct
    20041 atactgtttg aaacattggg taatattgcc gcaatttggt tcccactgtt caatttttct
    20101 aaaaaagatt ctgccattaa ttccccggtc tctacggggt ccgtatacac ataagaattt
    20161 ttatcgccat ttatttcaga ggtaggaaga gaatttatga ccacaacggg gatattcaag
    20221 tcctttaatt tgctatctat cttatgatac tccaggggat atataacaag ggcatctatc
    20281 ccatcgtcct ttaggctatc gatgaacata gcctgtttcg agatactggt attcttcgcc
    20341 gttatcttgt aggtaagatt aatgtccatt tcctctgcgg ctgccttcgc accctgaaga
    20401 aaattattgt tggtatcctg ggaatcctcg gacaaaacaa tagcaatgtt tagggttttt
    20461 gcaccgctac aaccgcccaa tatgatggaa aatactataa ctagcgcaag ataggcagac
    20521 agacgataac gcatgtaccc tcatcctcct cactttcaat cctcagtcca tatccgtctc
    20581 cgtagaggag ctgaattctg ttatggatat ttctcagggc aattccatgg gccttactgc
    20641 cgggcttgga atctagtgtc cctgtagtat tggccaggga tcggcgtaac tcctttaggg
    20701 aatcttcggt cattcctatc ccattatcct tgaccgagat cttcagcagt ccgatatggg
    20761 tatacgcctt gacaaagatt tttccgctgt ccaaggtaga taggccatgt tctatagcgt
    20821 tctcgattat aggctgcagg atcatccggg gacagaggaa ggcctctgtt tctggtccac
    20881 actctatata gaattcgaat ttattcccat agcgcaggga ttgaatatgc acatatctat
    20941 ccacatactc cagttcaaag gagaggggga ttgtcgatgc cgtcgtggag gcattgagct
    21001 taaagatgtc ggtgagggcg gtaatcatct ctatggcccc ccgctttttg ccgtcttcaa
    21061 tcatccagat gatggatcct aggatattat agagaaaatg gggggtaatc tgggtctgga
    21121 gggaggcgaa ttcggccttc ctaagctcga tttgttttag ttcctgttcc cgcagaagcc
    21181 gtttgatata gacaatcaga cgattgatgg attctgccag catattaatc tccaaaatag
    21241 aattatcctc atcaaaagtc acgtccagat gcctatcgtt aataccataa attttttcgc
    21301 tgatatcctt taggggcagg gtgattctcc tggtaaacaa atagctggtg gggagtatga
    21361 gcataagcat aaacacggat atcaaaatta aacgggtgac atagaatctc catgtatagc
    21421 caagatcccc cagatatgcg gcattgacta cataccagtt ttcgtaaggc atgtaatcta
    21481 cgtggttcat atactttttc ccattgatct cgctggtata ggtatggttt atagattggg
    21541 ttaaaacacc ttgaaaatcc tctttccaca agcccatcaa taccagttgc tgcttcgtat
    21601 ggtagataag gtcttcattg cttactatat agacatatcc cgaactcttg gattgtgcgc
    21661 cggaacatac agccgcaatg gacgagaatt ttatgtccat cagaagcact gcgggatgaa
    21721 tcgccccatc ctttttatag gcgatgcgcc tgctcaggga catgacccaa tcgtattttc
    21781 ctgtaaaaag gttttgcacg tggggtacgg taaatttatt gatggtgttg gcctcggcaa
    21841 accagctttg ctccttggcg tcgacggttg gttttaaccg attttccgtc tgggaataga
    21901 taaggtcacc gtcataatca aacagggcaa tggtaacaat gtcattgttg gtggaaaaaa
    21961 tcgttgacag cttcgcattt aaatccccgc tatctagatt gtccgcgtgt aaaattgtca
    22021 cagcaacatt attgaaggtg ttgcggaagg aggacatagt tagctcaagg tttgatttta
    22081 attgggaagt agtcgtggta agctgcgagg tataacccct gtttaggatg ttcgcaaaga
    22141 gaaaataatt gagtacaaat aaaagcccga tggctatgat aaacaggaag agtatggagc
    22201 catataaaag cttgctgacc gatcgttccg cccttaatat tttcattggg actgtttctc
    22261 cctttccctt cccttacgcc tatattccac cggcgaagtt tttacatact ggcggaaacg
    22321 gtaggaaaaa taattggggc tggtgaagcc cacctcccgt gcaatctggg aagacttttt
    22381 gtcagtattt aagagaaggg aacacgcctt ctgaatccgc aacgatgtaa ggtactgcaa
    22441 aaatgtttgg gaaaaatccc gtttgaacaa agcggaaaaa taggaggcac ttatatctag
    22501 gtgatcacag atcctatcaa gggagagctc cggatccgta tagttttcgt ctatataact
    22561 cttggcgtgg gccataagca gcgtgccggt acagtaattc ttgtcctgta tctccctgtt
    22621 tatatattcc gctatcagag taataagctg aatccatccg tttatatcct gaaagtgatc
    22681 atatatctgg tgtaccggaa attttgaaag cacatcaaca tcaaaatcat ccgccaatcg
    22741 gagcaattgg ctgtttatct ccgcctgggt agagctgagt cgctgctctg acagctgggc
    22801 ctcggccaag gattgcgcta tattatcaaa aactcctgcg atatcgtcct tttcaccgtt
    22861 gatgataacc gattcgtcca atgtcaggtc caatagggtc ggcgattcgt cctgttctaa
    22921 ctggagatcg gaatagcaat aaacccgttg ctcgttgttg acaaaatcca gattcaaggc
    22981 cctcaccgcc tgcagataat aaacattggc gtttctcaaa gaactacacg ggttgctgat
    23041 accgacgatc agctttagcc cccagagctt ctcggctgcc gccacgatcc tgtcgcatag
    23101 cgcgatcaaa ttccttttat ggctgaaggc atagatcacc aatctttcct gatactgaag
    23161 gatatatgaa gtaaaatccg cattaatctg ctcttctaca agatttcgca gggccagcca
    23221 catgatctcc tgttttccgc taaaatgccg atcaattaat tttctgcctt ttcccgtgga
    23281 ctcggtctgt atggcaatta tggaaaatgt ggacctttgg ggcaaatcgt aggatcccac
    23341 ggccatatta aaatattcgt cggatacacc cttacttagg atttcgatca aaaaggagga
    23401 gcgtaaaaga tccatactct ccttaagctg ttgtctgagg gtgtccatat cgttgcgtat
    23461 ggcatactgt ttatctaaat gtgctttggc cttacccaat gatttttcta tgatctcctt
    23521 ggtaacaggc tttataataa agtcaagcac gccgtattgg agcgccagtt gcgcatagtc
    23581 gaattcgtca tgaccgctta ttattaaaaa gcagatggaa ttgtcctttt catgtacccg
    23641 ctttatcatt tcaaagccat ccatataggg catacgtatg tcggttataa tgaggtcggg
    23701 ctttacctgg tccactattg atagcgcctc ttggcccgat agggcctttc cagccaaggt
    23761 aaatccgcag gtttcccaat ctatattcct gactatattt tcaagggttt caatttcgtc
    23821 ttctacaaga actagtttat acactgataa gtatcctttc aaagaatgat atcatgcgcg
    23881 aataatagcc ggatccagaa caaatacggc ataatgaatt atagtatata gggtctaaaa
    23941 aagcaacaga gggtagttat tgccagcgtt taagccggga tccggtcgag ttttatcaga
    24001 tagcaatccc caccatatat acacatctaa atccctttga ttgatgggag gcgggtttat
    24061 gggttctttc ccctctattc aattcaacga cccaatttta cccaacggca tatgaaaata
    24121 agagtatttt gatattcctg tagtaa
//
LOCUS       DASRNX010000171         7680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_142120,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000171 DASRNX010000000
VERSION     DASRNX010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142120"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1280)
                     /gene="ugpC"
                     /locus_tag="VFD89_06810"
     CDS             complement(165..1280)
                     /gene="ugpC"
                     /locus_tag="VFD89_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HZJ57937.1"
                     /translation="MASVTLKQMYKVYPGGVTAVSDFNLDVADKEFIVLVGPSGCGKT
                     TTLRMVAGLEEITEGELYIGDKLMNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGLK
                     LRKTPKAEIDRRVKEASRILDIEHLLNRKPKALSGGQRQRVALGRAIVREPKVFLMDE
                     PLSNLDAKLRVQMRTEITKLHNRLQTTFIYVTHDQTEAMTMGTRIVVMKDGFIQQVDS
                     PQNLYDYPVNLFVAGFIGSPQMNFIDVTLTKEGDAVYAVFGENKIRIPEGKIKKLKDS
                     SYTGQEVTMGVRPEDMHDEEIFLQSAADSTIKAYVDVVELMGSETYLYLNVAGANITA
                     RVDPRSTARAGDTIRIALDPNRLHFFDKDTEETLLVR"
     gene            complement(1525..1716)
                     /locus_tag="VFD89_06815"
     CDS             complement(1525..1716)
                     /locus_tag="VFD89_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZJ57938.1"
                     /translation="MKVFVDQDLCISCGLCISVCEDVFSWNEYDKAEAIDEDVPVELE
                     EDVEEAVETCPTEAIQYQE"
     gene            complement(1930..3699)
                     /gene="hflX"
                     /locus_tag="VFD89_06820"
     CDS             complement(1930..3699)
                     /gene="hflX"
                     /locus_tag="VFD89_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZJ57939.1"
                     /translation="MIQGNIDGLSKATIAELESLFDIVVSKDEFCNHEIIEKICRLTT
                     DTNREISVYIERSGRVLDVSVGDVGQVSLPYMSARRSKVRLNGIRCIHTHPGGSGVLS
                     EVDLNALTSMRLDSMAAIGVLKGKFNQGYAAFINPPDSDREITIAGPLSQAEFCHSEL
                     MEYIYERDKAIQHRDIVEVADDGQETAILVGLDTYGEGAASLEELEQLAQTARAVVIS
                     KVIQNRRVPDAATYIGKGKAEELALLCRAENVNLVIFDDELSPSQIRNLGDILGIRII
                     DRTTLILDIFAGRAESKEGKLQVELAQLRYRLPRLMGMGLILSRLGAGIGTRGPGEKK
                     LETDRRHIRRRIGEIEEELDKVRDRRHALRERRERNQIPIVALVGYTNAGKSSLMNAL
                     SGSSVLVEDRLFATLDPVHRGISLPNGQEILLADTVGFINKLPHDLIEAFKSTLEEAV
                     YADLLLHVVDAGSPDLQEQMSVVEHLLDSLGCNQTIITVYNKMDLVGEEDYGQPAKKP
                     AAYISATERTGLEDLMGLIAENLSIKRRRMELLIPYEDGHMLSPIHDQGQIFKEEYRE
                     DGIYFYVELDPVSYGRLVEYEIK"
     gene            complement(3865..4437)
                     /locus_tag="VFD89_06825"
     CDS             complement(3865..4437)
                     /locus_tag="VFD89_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HZJ57940.1"
                     /translation="MWIYEKKLQFPIKIKNPNPQMATLVVSQYGGPDGELAASLRYLS
                     QRYIMPIPEGKGLLTDIGTEELAHFEMVGAMVYQLMKGASAEEIKASGAGPYFTDHGR
                     GVYPTAAAGSPFTAAYIQSKGDPIADLNEDLAAEQKARGTYEWLLRMADDPDVIEPLK
                     FLRQREIVHYQRFGELLMKVQDYLESKKYF"
     gene            complement(4454..4711)
                     /locus_tag="VFD89_06830"
     CDS             complement(4454..4711)
                     /locus_tag="VFD89_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HZJ57941.1"
                     /translation="MKINDRDQLLDEIRQLEFMAVELNLYLDNNPNDQRAVRDYNSFT
                     KQLMAVKDEYQRRYGPLSNFGTALSQFPWAWINDPWPWDID"
     gene            complement(4727..4939)
                     /locus_tag="VFD89_06835"
     CDS             complement(4727..4939)
                     /locus_tag="VFD89_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HZJ57942.1"
                     /translation="MIDVYEHDQPISVLGSDCIVLPFPPNSKLARAFFVVQRYTTSLP
                     PSEALNRGTMFPELLTPWPSHQGKGM"
     gene            complement(5186..5524)
                     /locus_tag="VFD89_06840"
     CDS             complement(5186..5524)
                     /locus_tag="VFD89_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZJ57943.1"
                     /translation="MIISRNDKGVYVQPKGSISFLTVDAIKSQVYDFIGKGDTALLIN
                     LSKVDFLDSAGLGLLLSFFKYMKSHNGEMIVEYPKLGVQKLLEMTRMDELFVVKKKPE
                     STTGSWGEFI"
     gene            complement(5560..5847)
                     /locus_tag="VFD89_06845"
     CDS             complement(5560..5847)
                     /locus_tag="VFD89_06845"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HZJ57944.1"
                     /translation="MRVFVAVAEIDIRLYNSQSLKEKRQILKSLVSKVRNQFNVSIAE
                     IGVLDAWQRANLGFACVANNSIQAEKQMNRVLEYIEQDIRLEVIDIQKEIL"
     gene            complement(5844..7484)
                     /locus_tag="VFD89_06850"
     CDS             complement(5844..7484)
                     /locus_tag="VFD89_06850"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HZJ57945.1"
                     /translation="MKIAVAQYNPKVGDLEGNASKIIDFIAEAKEQKCDLVIFPELSI
                     TGYPPRDLLLRSAFLKACNRALHRIIGYTGEIGVIVGTVMAGPRDDLLCNSAILIEDG
                     DIVGRVDKSLLPNYDVFEEARYFRPSEQIKCLQFKGKRLGVSICEDIWNDEDLFGHLK
                     YPRNILDELYEDEPDIFINLSASPYHYGKHQIRMDMIAHYTRKYGIPFIYANQVGGND
                     ELVFDGSSMVYDDSGQLILNAKAFEEDLIIYDTGKAYPKLLIHKEDISWIYRGLVLGV
                     KDYFHKTGFKRTLLGLSGGIDSALLACIAREALGPENVLGVFMPTRYTSEESRKDADD
                     LASNLGIEYRTIPIEDTFHEYLKIFNGDGQARGDLAEENIQARIRGNMLMFIANREGR
                     VLVCPSNKSEIAVGYSTLYGDSCGGIAVLGDILKTDVYELCRYINSKDEIIPNNIIVK
                     APTAELKDNQKDEDSLPPYSLLDEVLRMYIEENVPLEEIIQKGYDRKTVYRTINLIDR
                     AEYKRRQIPPIIKLSHGDFGIGRKHPVVHGFRPEVYEP"
     gene            complement(7489..>7680)
                     /locus_tag="VFD89_06855"
     CDS             complement(7489..>7680)
                     /locus_tag="VFD89_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57946.1"
                     /translation="AVIGMESIQDRDDFQILEELASVSGEDIPPGLMELKDKPILHRT
                     TLAAGNMRQAINNFLELDG"
BASE COUNT         1966 a         1962 c         1439 g         2313 t
ORIGIN      
        1 agctaagcta ctttgatacg tacaggcgtg tccagttcgc ctctaccgta tagccctgtt
       61 aagggcacaa cgctactttt atgggattct ccaattcatc tcacgactta agtcacgagt
      121 gttcttgagg agcctaataa aaatttcata gatatttgtc tgtactatct gacaagcagg
      181 gtctcctctg tgtccttatc aaagaaatga agcctattgg gatccagcgc tatcctgatg
      241 gtatcaccgg ctcttgcagt agatcttggg tctaccctgg cggtaatgtt agcacccgct
      301 acgttgagat atagataggt ctcagaaccc atcaattcaa caacgtcaac atatgcttta
      361 attgtgctgt cagctgcact ttgtaggaaa atttcctcat catgcatgtc ctcaggcctt
      421 acacccatgg ttacttcctg cccggtatag gaggagtcct ttagtttctt gatcttgccc
      481 tcgggtattc tgatcttgtt ttcgccaaat acggcatata ctgcatcgcc ttctttggtt
      541 aaggttacgt cgatgaagtt catctgagga cttccgataa atccggcaac aaaaaggttt
      601 acaggataat cgtataggtt ttgaggagaa tcaacctgtt ggatgaatcc gtccttcata
      661 acaacgattc ttgtacccat ggtcatagct tctgtttggt catgggtaac gtatataaag
      721 gttgtctgta atctgttatg gagcttggtg atctcggttc tcatttgaac cctcaactta
      781 gcatctaagt tagatagagg ttcgtccatt aggaaaactt taggttcccg aacaatagca
      841 cggcccaagg ctaccctttg tctttgtccg cctgataggg ctttgggttt tctgttgagc
      901 agatgctcaa tatccagaat ccttgatgcc tctttaacac gacggtctat ttcagcttta
      961 ggggtctttc tcagttttag tccaaaagcc atattatcat agacggtcat atgaggataa
     1021 agggcatagt tttggaaaac cattgcgata tccctgtctt ttggggctac gtcattcatt
     1081 aacttatcgc caatgtaaag ctccccttca gtaatttctt ctaaccccgc gaccattctc
     1141 agggtagtgg ttttcccaca tccggaaggt cctacgagaa cgataaattc tttatcggct
     1201 acatcaaggt taaaatcact gacggcggta acgccccctg ggtaaacttt atacatttgt
     1261 ttcagggtta cacttgccat taaaatccct ccacataatt attttgcatc aattgtgatg
     1321 caaatcaagg gttcatgtta ttataagaca attatatccc tataaggaca atctgactat
     1381 agctatatta ccaaaaatca gggtggtttt tttgaaaaga ttgcataaag aaaatacggc
     1441 aacttacact aaatgggtta aatgtataat atatgaataa cccaccatag gaatatctct
     1501 ccaaggtggg ttatttgagt tttgtcattc ctgatattgg attgcttcag tgggacaagt
     1561 ctccaccgcc tcctcgacat cttcctctaa ttccactgga acgtcttcat cgatggcttc
     1621 cgccttatca tattcgttcc atgaaaatac atcctcgcat acactgatgc acagcccaca
     1681 gctgatgcac agatcctgat caacaaacac cttcaaggtt atccctccca tccttattgt
     1741 tatatgatta ttccccataa agaattatag gtccgcttta acggccgcga ctaatgccag
     1801 aatggttcta ctcataatag atatagggac tgatcttagt agagcccact tcgcgggtga
     1861 taatcaccat atgatttatt cgtacattta tacctctaat ctatacttca aaacaaaaaa
     1921 tatactccct tatttgattt catactccac aagacgtcca taggatacag ggtccaactc
     1981 tacataaaaa tatatcccat cctccctgta ttcttcctta aaaatctgcc cctggtcgtg
     2041 tattggagac agcatatgcc cgtcctcata gggaataagc aattccatcc ttcgtctttt
     2101 gatggatagg ttttctgcta tcaaccccat aagatcctcc aaacccgtcc tttccgtagc
     2161 ggaaatgtat gcggcaggtt tttttgcagg ttgcccgtaa tcttcctctc ccacgagatc
     2221 catcttatta tagaccgtaa tgattgtttg gttgcaaccc aaagaatcca gaaggtgttc
     2281 caccacgctc atctgttcct gtaagtcagg tgatcctgca tccaccacgt gcagaagcag
     2341 atcggcgtag actgcctcct ccaatgtgga tttgaaggct tcaataagat catggggcaa
     2401 cttattaata aagccaacag tgtccgctaa gagaatttct tgcccgttgg ggagactaat
     2461 ccccctatgt acggggtcca gagtagcgaa taacctatcc tcaaccaaca cgctactccc
     2521 gcttaatgca ttcatcagac tgcttttacc ggcatttgtg tagccgacca gggctactat
     2581 aggtatctga tttctttctc ttctttcccg aagggcatgt ctcctgtccc tgaccttatc
     2641 cagttcttcc tctatctccc caatccttct gcgaatgtgc cggcgatcgg tttccagctt
     2701 cttttccccg ggcccccttg taccgatccc tgcgcccaac ctggaaagta tcagacccat
     2761 ccccattagc cttggcaatc gatacctcaa ttgggccagt tctacttgga gcttaccttc
     2821 cttggattcg gctctcccgg cgaaaatgtc caaaatcagg gttgtcctgt caatgattct
     2881 gatccctaaa atatcgccta ggtttcgaat ctgggacggg gaaagttcat cgtcaaaaat
     2941 aactaggttc acattttcag ccctgcacaa tagggcaagc tcttctgcct ttccctttcc
     3001 tatgtaggta gccgcatccg gtactctcct gttttgaatt accttgctga tgacaaccgc
     3061 ccttgctgtt tgcgccagtt gctccaattc ctctaacgag gcggcaccct caccgtatgt
     3121 gtccaatccc accagtatag ccgtctcctg cccatcgtct gctacctcta ctatgtcacg
     3181 atgctgaata gccttgtccc tttcgtatat atactccatc agctcactgt ggcaaaattc
     3241 cgcctgggac aggggccccg ctatggtgat ctctctatca ctatccggcg gattaataaa
     3301 agctgcatac ccttgattga acttgccttt caatacccct atggccgcca tggaatccaa
     3361 cctcatagat gtcaaggcat ttaaatccac ttcagatagg actccgctgc cgcccggatg
     3421 agtatgaatg catcttatcc cgttaaggcg caccttgctc ctgcgggcgc tcatgtaagg
     3481 caatgaaacc tgacctacat ctcccactga aacatccaaa acccttccgc tgcgctcaat
     3541 atataccgat atctccctgt ttgtatccgt cgtcaaccta catatttttt cgataatctc
     3601 atggttacaa aactcgtcct tggatactac gatatcaaat agagattcca attccgcgat
     3661 tgttgccttc gataggccat caatatttcc ctgtatcata tcggtgtctc tgcctccttc
     3721 caagtataaa ccccatattc cctctaaatt atacatcaaa aggataaaac aaccaagtcc
     3781 ccccgtaggg ccggcccata ttttaaatcc catggaaata acgtatgggc cgatttttct
     3841 atgcgggcgg taatcatata atacctaaaa atattttttt gattctaagt aatcctggac
     3901 cttcattaat aattctccaa acctttgata atgtactatc tcccgttgtc ttagaaattt
     3961 caggggttct ataacatcgg gatcgtccgc cattctcaag agccattcat aggtcccgcg
     4021 ggccttttgt tcggccgcca agtcttcatt taagtccgct atgggatcgc cttttgattg
     4081 aatgtatgct gcggtaaatg gggagcccgc ggctgctgta ggatataccc cccgcccatg
     4141 gtcagtaaaa tatggtcctg cacccgatgc cttgatctct tcagccgagg cacccttcat
     4201 cagctgatac accatagcgc ccaccatctc aaaatgggca agttcttcag tgcctatatc
     4261 cgtcaacagt ccctttccct cgggtattgg catgatatat ctttgggaca gatatctgag
     4321 ggatgcggct aattcgccgt ccgggccccc atattggctg actacaaggg ttgccatctg
     4381 tgggtttggg tttttgatct taatgggaaa ttgcagtttt ttttcatata tccacatata
     4441 aatattcctc cctctaatct atatcccatg gccatgggtc gtttatccat gcccagggaa
     4501 actggctcaa tgctgttcca aaattgctca agggaccata ccttctttgg tattcgtcct
     4561 ttacagccat taattgcttc gtaaaggaat tatagtccct gaccgccctt tggtcattgg
     4621 ggttattgtc taaatacagg ttgagctcca ccgccataaa ttccagttga cgaatttcat
     4681 ccaaaagttg gtctctgtcg ttaatcttca tcaaaaacat cctcctttac atacctttcc
     4741 cctggtgact gggccagggg gtcaacaatt cagggaacat agtcccccta tttagggcct
     4801 cacttggggg gagactggtc gtataccttt ggacaacgaa gaatgcccgg gccaattttg
     4861 aatttggcgg gaaaggcagt acaatacaat ccgatcccaa tacgctaatg ggctgatcat
     4921 gctcatatac atcgatcata tctttactcc tccatattaa tgttatgata ttaacatgct
     4981 atgtgaatat atggcaaaat gagcctgacc cgccggttat gtccttaaac tacccacagt
     5041 atgaaatcat gaaaaggtat aacaggatat gccgtgacaa tcctctaatg tgtctgggca
     5101 aagtttgtct gccgaaaaaa agaaaacatc ggacatatat tttcatgtcc gatgttttcc
     5161 cacctatata ggctttatat aaaccttata taaactctcc ccagctgccg gttgtggatt
     5221 ccggcttttt cttaaccaca aacagctcat ccatacgggt catctccaat agcttctgca
     5281 cccccaattt agggtattcc acaatcattt ccccgttatg acttttcata tacttaaaga
     5341 aagagagcaa aagtcccaac cccgcactgt ctaaaaagtc aacctttgag agattgatta
     5401 acaaggcggt atcccctttt ccgataaaat cgtagacctg actttttatt gcatcaacgg
     5461 tcaaaaagga tatgcttccc ttgggttgga cataaactcc tttatcattc cgtgagatta
     5521 taataagtca tcccccctta ggaccgggta tcccatacct cacaatattt ccttttggat
     5581 atctatcacc tcaagccgta tatcttgctc tatatactct aaaactctgt tcatctgttt
     5641 ctcggcctga atagaattat tggctacaca ggcaaagcct aaatttgcac gttgccaggc
     5701 atccaaaaca cctatctccg ctatggatac attaaactga tttctcacct ttgaaacaag
     5761 actttttaat atttgtcttt tctccttcaa cgattgagaa ttatacaacc taatatctat
     5821 ttctgcgaca gctacaaata ccctcatggt tcatatactt ccggcctaaa accatggacc
     5881 acaggatgct ttcttccaat accaaaatca ccgtggctga gcttgattat tggcggtatc
     5941 tgtctgcgct tgtattcggc cctgtcgatc aagttaattg ttctgtatac ggtttttctg
     6001 tcgtatccct tttgaataat ttcttccaat ggaacattct cttcaatata cattctcagg
     6061 acttcatcca atagggaata ggggggcagg gaatcctcat ccttttggtt gtcctttagt
     6121 tctgccgtag gggcctttac aatgatgtta ttcggaataa tttcatcctt gctatttatg
     6181 tatctgcaaa gctcatagac atcggtcttt agtatatccc ctaaaaccgc tatgcccccg
     6241 caggagtcac cataaagggt ggaataacct acggcgattt ccgatttatt gctgggacaa
     6301 accagaaccc gtccctccct attggcaatg aacatcagca tgttgccccg gatcctagcc
     6361 tggatattct cctctgccaa atccccacgg gcttgcccgt ctccgttaaa aattttcaga
     6421 tattcgtgga atgtatcctc gattgggatg gttctatact ctataccaag gttggatgcc
     6481 aggtcatcgg catccttcct gctctcttcg gacgtatacc tggtgggcat aaatactccc
     6541 aggacgttct ccggacccag ggcctccctc gcgatacacg ccaatagcgc agagtctata
     6601 cccccgctta accccaagag agtcctttta aaacccgtct tatgaaaata atccttgacg
     6661 cccagcacca agcccctata tatccaagat atatcctctt tatggattaa aagttttgga
     6721 tatgccttcc cggtatcata tataatcaaa tcctcctcaa aggctttggc attgaggatc
     6781 aattgtccac tatcgtcgta aaccatactg gagccgtcaa atacaagttc gtcatttccc
     6841 cctacctggt tagcatatat gaaagggata ccatacttcc tagtataatg ggctatcata
     6901 tccatacgga tttggtgttt tccataatgg tatggagatg ccgataggtt tatgaatata
     6961 tccggttcgt cctcatagag ctcgtctaag atattcctcg ggtattttag gtggccgaac
     7021 agatcctcat cgttccaaat gtcctcacat atggacaccc ccagcctctt tcccttgaat
     7081 tgaagacact ttatctgttc cgagggccgg aagtatctgg cctcttcaaa tacatcataa
     7141 ttcggcaaaa ggcttttatc caccctccct acaatatccc catcctcaat caaaatggca
     7201 ctattacata gtaaatcatc tcgagggccg gccattacag ttcccacaat aactcctatt
     7261 tccccggtat agcctatgat tctatgaagg gctctgttgc aggcctttaa aaaggcactc
     7321 ctcagcagaa gatcccgggg cggatatccc gttatgctaa gctccggaaa gattaccaga
     7381 tcacattttt gctctttggc ctcggcgata aaatcgataa ttttagatgc gttgccctct
     7441 aaatcgccaa cctttggatt gtattgtgct actgctattt tcataatttt atccgtccaa
     7501 ttccaaaaaa ttattgattg cttgacgcat attaccggca gctagggtag tccggtgcaa
     7561 tatgggttta tcctttaatt ccatcaggcc cggcggaata tcttccccag atacagatgc
     7621 caattcctcc aatatctgaa aatcgtccct gtcttggatg gattccatgc ctatgacagc
//
LOCUS       DASRNX010000172         5924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_142205,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000172 DASRNX010000000
VERSION     DASRNX010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142205"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..1463)
                     /locus_tag="VFD89_06860"
     CDS             complement(234..1463)
                     /locus_tag="VFD89_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZJ57947.1"
                     /translation="MKSKSMKLDYKKTFILGLGFFSASIIWPIYNNYIPIMLESYIKS
                     GTFIGAIMTIDNIFAVIFQPLFGALSDKTNTRFGKRMPFLLVGVPLAAIFCSLIPWST
                     SLVTLMIFIIIMNFAMSIYRAPTVALMPDVTPSPLRSKANGVINFMGGFAAVIAYGLG
                     GFLYKVNTGYPFYMASVVMVAALALLYAFIKEPQDLKVEEKEKEIKKDVAKNRSLIFM
                     LLAIFFWFTGFNAVETFFSLYGQHILGIEPGTSSIVLTSFSLAFLVFAIPAGFVAGKL
                     GRKRTILFGIIGALLVFVPLIFIRNTLIIPILLVSGGIFWALININSYPMIVEMAPKG
                     LTGAYTGYYYFFSSAAAIVSPILFGWIKDLVNSYKPLFIYASMGFVLALVCMFFVGHG
                     DIKDVDRAKAGAAELGV"
     gene            complement(1478..2215)
                     /locus_tag="VFD89_06865"
     CDS             complement(1478..2215)
                     /locus_tag="VFD89_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HZJ57948.1"
                     /translation="MNMALIIAHRGASGCAPENTMAAFEEALKMGAEGIELDVHMTKD
                     GEVVVIHDHTIDRTSDGKGMVGGFSLEEIRGFDFGAWFDPKFEGARIPTLGEVFELLR
                     DWDGLLNIEIKSGPIFYPGIEEKLVHMVRDYDFPGRIIFSSFNHYSLRDIKVMDPNME
                     IGLLHMAGLVEPWKYAKDLGAEALHPLYYNVIPELVAGCKENGVKLNPFTIDDEKEIE
                     MIMRAGVDGIITNYPDRALNIKKRLSS"
     gene            complement(2762..4048)
                     /locus_tag="VFD89_06870"
     CDS             complement(2762..4048)
                     /locus_tag="VFD89_06870"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZJ57949.1"
                     /translation="MAIIRIGLLGLGNVGSGVTNIFYENMEKITEYIGAEIQVEKVLV
                     RDIEKPRQAILDRQILTTDPRDILENPNIDIVVELMGGIEPAFSYIKQAMENHKHVVT
                     ANKAVMATHCKELFEIAKANDVEIRFEGSVGGGIPLIDTITTKLAGNRIDGLVGIING
                     TTNYILTRMTREGMGFDKALRKAQEKGYAEADPSSDIEGQDAAYKLAILAATAFGLYI
                     DPEDIPCEGITKVTEAEIRYAAELGFAVKLLATAKNKEGGLEIHVHPAIIPDNHPLAG
                     VHNEFNALLVEGNAVGELMLYGKGAGSLPTGSAVVGDILDIIRMMRQSAKTGSSLVAG
                     RNNINVVGEDTGSYYIRITVVDKPGVLGVITTTLGKHGISIASVAQQSTGQRLVPVIF
                     ITHEVERAKLDEGLDQLRNFSDVVKEVACILRVEGY"
     gene            complement(4179..4961)
                     /locus_tag="VFD89_06875"
     CDS             complement(4179..4961)
                     /locus_tag="VFD89_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HZJ57950.1"
                     /translation="MRKLAKLFFLTLVISLTLFACNEYLKAPSPSEDDPRGVEFTLSG
                     RAKIQRILNDQDLYPERLIESIKRNPEIVDFAFDYLEKKGTYNENIDLSPKYTRGEIP
                     MLMQWDEDWGYAPYGQGVIGLDGCGPTALSMVYVGLTGDFSYNPQAVAKFSEDNGYLD
                     NKNDITLWTLMSEGAKKLGLESRELPLDENIMAREVCGGHPIICSMKPGDFTTTGHFI
                     VLYDYIDGEFFVRDPNSISRSQKRWSYSVIESQISNIWAFSK"
     gene            complement(5248..5322)
                     /locus_tag="VFD89_06880"
     tRNA            complement(5248..5322)
                     /locus_tag="VFD89_06880"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5288..5290),aa:Asn,seq:gtt)
     gene            complement(5394..5510)
                     /gene="rrf"
                     /locus_tag="VFD89_06885"
     rRNA            complement(5394..5510)
                     /gene="rrf"
                     /locus_tag="VFD89_06885"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(5738..>5924)
                     /locus_tag="VFD89_06890"
     rRNA            complement(5738..>5924)
                     /locus_tag="VFD89_06890"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1552 a         1511 c         1175 g         1686 t
ORIGIN      
        1 agctaagcta ctttgatacg tacaggcgtg tccagttcgc ctctaccgta tagccctgtt
       61 aagggcacaa cgctactttt atgggattca ccaattcatc tcacgactta agtcacgagt
      121 gttcttgagg agcctaataa atgaccgatt gactgggatt tcaaaggact gtaattgtcc
      181 gcattacagc ctataacatc tgattatatg tcccttgatc cttctcccag ttgtcatact
      241 cccagttctg cggctcccgc tttcgccctg tccacatcct ttatgtcacc atgccctaca
      301 aagaacatgc atacaagggc cagcacaaat cccatgctgg catagataaa taagggtttg
      361 tagctgttaa ccaaatcctt tatccaaccg aacaatatag ggcttacgat cgccgccgcg
      421 gatgaaaaga aataatagta tcccgtataa gcgccggtca ggccctttgg tgccatttca
      481 actatcattg gatacgagtt tatatttatg agcgcccaga atataccgcc ggataccaag
      541 agtattggga taatgagggt gtttcgaatg aatataaggg gaacgaacac caaaagggca
      601 cctattatcc caaagagtat ggtccttttc ctacccagct tacctgcgac aaatccagcg
      661 ggtatggcaa agacaagaaa tgccagggaa aatgaggtga gtactataga cgatgtcccc
      721 ggctctatcc ccaggatatg ctgtccatag agggaaaaga aggtttccac agcattaaac
      781 cccgtaaacc aaaagaatat agccaggagc atgaagataa ggcttctgtt ctttgctacg
      841 tcctttttga tctccttttc cttctcctcc acctttaggt cctggggctc ctttataaaa
      901 gcatatagaa gagccagggc agcaaccatc accactgagg ccatatagaa gggatatcct
      961 gtattcactt tatagagaaa gccgcccagg ccataggcta tcacggccgc aaaccctccc
     1021 ataaaattta ttacaccgtt tgctttgctc cgtaaggggc taggggtgac atcgggcatc
     1081 aatgccaccg tcggtgccct atatatgctc atggcaaaat tcataataat gataaatatc
     1141 ataagggtaa ccagggaggt ggaccagggt attagagaac aaaatattgc tgccagggga
     1201 accccaacca gaagaaacgg catgcgtttg ccaaatctgg tattggtctt atcgctcagt
     1261 gcgccgaata ggggctgaaa aataacggcg aagatattat caatcgtcat tatcgccccg
     1321 ataaaggtcc cgctttttat gtaggattca agcattattg ggatgtagtt gttgtagatg
     1381 ggccagatta tactggcaga gaagaaaccc aatcccaaaa taaaggtctt cttataatcc
     1441 agtttcatag atttcgactt catggaatta tcccccttta actgcttagt ctcttcttaa
     1501 tattgagggc cctgtcggga tagttggtta tgatgccgtc cactcccgcc ctcattatca
     1561 tttctatctc cttttcatcg tctatggtga agggatttag tttcaccccg ttttccttgc
     1621 acccggccac cagttcagga attacattgt agtacagggg atggagtgcc tcagcgccca
     1681 gatcctttgc atatttccag ggctccacca aacccgccat atgtaaaagt ccaatctcca
     1741 tatttgggtc cattacctta atatccctga ggctgtagtg gttgaatgaa gaaaatataa
     1801 tcctgccggg aaaatcgtaa tcccttacca tatggacgag tttctcttcg atccccggat
     1861 agaatatggg gccgcttttg atctcaatgt ttaagagccc gtcccagtcc cttaataatt
     1921 caaatacttc ccccaaggtt ggaatccttg caccctcaaa cttgggatcg aaccaggccc
     1981 caaaatcgaa tccccttatc tcctccaaag aaaatccccc taccatgccc ttaccatcgc
     2041 tggttctatc gatggtatga tcgtgaatga ctactacttc cccatctttt gtcatatgga
     2101 catcgagttc gatgccctcc gcgcccattt tcagcgcttc ctcaaaggca gccatggtgt
     2161 tttcaggagc gcaacctgaa gcacccctat gggctattat caaagccata ttcatacacc
     2221 ccttctgttt ttattatata tgttatgaaa agaccttccg tcgcaaaacc agctgagcta
     2281 tctgattttg tgttatagca acggattgtt cgtaacatac gaattaagga taatgtttta
     2341 cgacttctgc ataccatcgt aggatgcttg tcgtaaagca tataggcggt cccggaaaaa
     2401 aagccttcta acgatgcatt aaatcgcggt atcctttgtc cggcacatct tctagtcgaa
     2461 tatatgtcgg taatggttgt tgggcaatct acatcgtcgc gagggtaact gaatagttgg
     2521 aattgttata gaatacctta tatctattct atgaacaaac aaaaaatcct ctgcttttgt
     2581 acaaaaaagc aaaggatttt tgcccataat ccatatttgg tgttatggga tgaaattatt
     2641 atacacataa cgaaagatat ctgtcactat aagatccatg aaagcacctg ttcttctctt
     2701 aagggatgac caccgcttgg tataaagatc aaagaaaata ttatatagcg cccaagatgc
     2761 cctagtaacc ctccaccctc aggatgcatg ccacctcttt tactacatca ctaaagtttc
     2821 tcagctggtc gagcccttca tcgagctttg ccctttccac ttcatgggta ataaatataa
     2881 ccggtacgag cctttgcccc gtactctgtt gggccacaga cgcgatacta atgccgtgtt
     2941 tccccaaggt ggttgtaatc actcctaaaa cccccggctt gtccacgacc gttatacgga
     3001 tataatagga cccggtatcc tcacccacga cgtttatatt atttctgccg gccacaaggc
     3061 tgctgccggt cttggcagat tgtctcatca ttcttattat atccaaaata tcgccgacta
     3121 ccgcactgcc ggtgggaagc gatcccgcac cttttccata cagcattaat tctcccacgg
     3181 cattgccctc tacgagcagc gcattaaatt cgttatgaac ccctgccaag ggatgattgt
     3241 ccggtatgat ggcaggatgg acatgtattt caaggccccc ctccttgttc ttggcagtag
     3301 ccaatagctt gactgcgaat cccagctccg ccgcatagcg gatctccgcc tcggtcacct
     3361 tcgtgatccc ctcacagggg atatcctcgg ggtctatata cagcccaaaa gcggttgcgg
     3421 ctaatatcgc cagcttataa gctgcatctt gcccttctat atccgacgat ggatcggcct
     3481 ctgcataccc cttttcctgc gcctttctta aggccttatc aaatcccatg ccctccctgg
     3541 tcatacgggt aaggatataa tttgtggtcc catttataat tcccaccagt ccgtcgatcc
     3601 ggttacccgc aagtttagtg gttatggtat caattaacgg tatccctccg cccacgctac
     3661 cttcaaatcg aatttctaca tcgttggcct ttgctatctc aaacaattcc ttgcaatggg
     3721 tagccataac agctttgtta gcagtaacta catgcttatg gttttccatg gcctgcttta
     3781 tatacgaaaa tgcgggttct atacccccca tcagctctac cactatatct atatttgggt
     3841 tttctaagat atcccgggga tcggttgtca gtatttgccg gtccagtatg gcctgcctgg
     3901 gtttttctat atccctgact aagacttttt caacttggat ttcggcacct atatattccg
     3961 taattttttc catgttttca taaaagatgt ttgtaacccc gctgcctaca tttcctaatc
     4021 ccaataatcc tattctgatg attgccaacc caatcttccc ctctctatct ttggtaaaca
     4081 attttattca ttctaacatc gattccggag atattcaacc ggactttgat aagcgcgtcg
     4141 cctttcccca tgcccagggg atcgaatcat atcaaggctc atttggagaa tgcccagata
     4201 ttgctgatct gagattcaat cacggagtaa ctccacctct tttggctcct gctgatactg
     4261 ttagggtccc tgacgaaaaa ttctccgtca atataatcat aaagaacgat gaaatgtccg
     4321 gtggttgtaa aatcccccgg cttcatactg catattatgg ggtgaccgcc gcatacctcc
     4381 ctggccataa tattctcatc cagcggcaac tccctggatt caagtcccaa ttttttggct
     4441 ccctccgaca tcagcgtcca cagggttata tcatttttgt tatccaagta cccattatcc
     4501 tcgctaaact ttgccaccgc ttgcggatta taggaaaaat cccctgtaag gcctacatac
     4561 accatagaaa gggcggtagg tccgcatcca tcaaggccga taactccttg tccataaggg
     4621 gcatagcccc aatcctcgtc ccactgcata agcatgggaa tttcccccct tgtgtatttt
     4681 ggggaaagat caatattttc gttgtaagta ccctttttct caagataatc gaaggcgaaa
     4741 tccacgatct cgggattgcg ctttatagat tcaataagcc tttccggata gaggtcttga
     4801 tcgttcagta tccgttgaat cttggctctg ccggatagag taaactctac accccgtgga
     4861 tcatcttcag aaggggacgg ggcctttaga tactcgttac aagcaaacag ggtgagagat
     4921 attaccagcg tcaagaaaaa cagtttagcc aacttccgca tatatcatta ctcctcataa
     4981 tttcctctta aagaaccggt taccaaataa tctactcatc tcagtcttcg cattccatct
     5041 agcctatgca gtctatttag cctttggatt ctatccccgg ggatatcccc atagggaatt
     5101 ataccacttt tagaacattt ttaatacacc atgtgaccca aatacccttg gcagattaac
     5161 ggttgaagga tccacctggc cgtggctttt gcactttaac gtaaaaagcc cctttagcga
     5221 tttgctaaag ggacttttat atacaattgg ctccccaggc tggattcgaa ccagcaaccc
     5281 ctcggttaac agccgagtgc tccaccgttg agctactgag gaatattaag atatagaaac
     5341 atccccatgt ttatgggata tctccctagg gggatgaact tatttaaatt agaatccggc
     5401 agcgacctac tctcccaggg ggtctccccc caagtaccat cggcgctgaa gggcttaact
     5461 gctgtgttcg ggatgggaac aggtgtatcc ccttcgccac cgccaccgga aatttgctag
     5521 aggttagaaa ttagaagttg gaagtcggat gcttttaggt ctgcccttag ggtctgtatc
     5581 atacacacgt ctccggatta ctaactctac ttctctataa tctttatagg tatatacaat
     5641 cctttgcctc gaaaatattc aagcctttag atgtagaata tatcgctgcg cgaatattcg
     5701 tgtatacacc ctcaaaacta cataatgaaa agttttattg atcaagccct cgacctatta
     5761 gtatcggtca gctacatgca ttgctgcact tacacctccg acctatctac ctcgtaatct
     5821 acaaggggtc ttaatccata aaggatggga aatcttatct tgaggggggc ttcacgctta
     5881 gatgccttca gcgtttatcc cgtccgtact tggctactca gcta
//
LOCUS       DASRNX010000173         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_142222,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000173 DASRNX010000000
VERSION     DASRNX010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142222"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..517)
                     /locus_tag="VFD89_06895"
     CDS             complement(239..517)
                     /locus_tag="VFD89_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57951.1"
                     /translation="MSILLSTLKEVNAMNVYSKDNLAKRLDTSREVVDHILFKLERMG
                     YIKEESIAISKCSSCPSFKNGCMGPVAGRPINALTITNKGKRFLGTKN"
     gene            complement(538..>2377)
                     /gene="feoB"
                     /locus_tag="VFD89_06900"
     CDS             complement(538..>2377)
                     /gene="feoB"
                     /locus_tag="VFD89_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HZJ57952.1"
                     /translation="GGYSEDEAVARDYILKGNHDIIINVVDATNLERNLYLTTLLMEM
                     GANIIIALNMVDDAEKKHIKVDYERLSEEIGVPVVPTIASRSRGIDELMQKTVDAMGG
                     SNHKRSISYGKEIDAKVALLKDLIEKHQTDREYPSGWLAIKLLENDEGIIEKYHDNDN
                     FKGKVLAEIKKTADEILATSGMEPEMVILDKRYEFVSDVVRKAVARPKEDIETVTDKI
                     DRVITHKYFGIPIFALIMFIIYQLTFTIGKDILGEGLAEGIEGLGEVVAGWLGAINAP
                     EILTLFISDGLFGGVGAVLEFIPIIMVMYMLFGILEDSGYLARAAYIMDRSMRALGLH
                     GKTFISMLIGTGCNVPGIMSTRTLDNKKDRMIAILINPFMSCGARLPIYMVFIAAFFP
                     KYGGLVLFSLYAIGIAVALIMGKLFSKVLFKGEESFFIMELPPYRLPTIKGIGLYMWE
                     QVWSFIHRAGTVIFLVVSLLWVLSILPYGVEPYSEASLLGMIGSFIAPIFRPAGFGTW
                     QASVGLFAGIAAKEAVVATLGMVYAGVEQGARLITSIQGVFTPLSAYSFLIMSLLYTP
                     CAATIAIIRKETNSKGWAWFAAVYTFVIGWMGAVLVYQVGKLFGLG"
BASE COUNT          636 a          639 c          463 g          639 t
ORIGIN      
        1 agctaagcta ctttgatacg tacaggcgtg tccagttcgc ctctaccgta tagccctgtt
       61 aagggcacaa cgctactttt atgggattca ccaattcatc tcacgactta agtcacgagt
      121 gttcttgagg agcctaataa aatacatagc aaaacacttt gaatttcaaa agcccggatt
      181 atcggcattg cttctggggc tccataaaat atacccggcg gaggcttccc gatattggct
      241 aatttttcgt gcccaagaac cttttaccct tgttcgtgat agtaagagca ttgatgggtc
      301 tccccgcgac gggtcccata cacccatttt taaagcttgg gcaggagcta cacttactta
      361 tagctatgct ctcttccttt atatagccca tcctttccaa tttaaacagg atatggtcta
      421 caacctcccg gctggtatca agccttttgg ccagattgtc cttagaatag acgttcatgg
      481 cattgacttc ctttaaggta ctcaacaata tgctcaccaa tcattacccc ccaacattca
      541 accgagtcca aacaacttac cgacctgata gaccaagacc gcccccatcc agccgataac
      601 aaaggtatag acagccgcaa accaagccca gcccttggag ttggtttctt tacgaatgat
      661 tgcaatggta gctgcgcacg gggtatacaa aaggctcatt atgaggaagg agtatgcact
      721 taggggagtg aatacaccct gaatggatgt aatgagtctg gcgccttgtt ctacccccgc
      781 atataccatt cccaaagtag ccaccaccgc ttccttggcc gcaatgcccg caaagagacc
      841 tacggatgct tgccatgtcc caaaccctgc aggcctgaag atcggcgcaa taaagctccc
      901 tatcatcccc aaaaggctgg cttcactata gggttccacg ccatagggaa gtatggatag
      961 gacccatagg aggctgacta cgaggaatat aacggtcccg gccctgtgaa taaaggacca
     1021 cacctgctcc cacatataaa ggccgatccc tttaatggtg ggcagcctat aaggaggtaa
     1081 ttccattatg aaaaatgatt cctcaccctt aaacaagacc ttggagaaca gtttacccat
     1141 tattaaggca acggctatcc ctatggcata tagactaaac aataccaatc cgccgtactt
     1201 agggaaaaaa gccgctataa acaccatata gatgggtaat ctggcaccgc aggacataaa
     1261 gggatttatg agtatcgcaa tcatcctgtc ttttttatta tctagggttc tggtagacat
     1321 tatgcccggg acgttacacc cagtacctat cagcatagat ataaaggtct tgccatggag
     1381 ccccaaagcc ctcatggatc tgtccattat ataggctgcc ctggctaaat atccgctgtc
     1441 ttccaatatc ccaaacaaca tatacataac cattatgatg gggatgaatt ccaacacagc
     1501 accgactccc ccaaaaaggc cgtcggatat gaacaaggtc aaaatttccg gagcattaat
     1561 agccccgagc caccctgcaa cgacttcccc cagcccctct attccctcag ccaaaccttc
     1621 gcctaaaata tccttaccga tggtgaaggt gagttggtaa attataaaca taataagggc
     1681 aaatatgggg atcccgaagt acttatgggt aatcaccctg tcgatcttgt cggttacggt
     1741 ctctatatcc tctttcggcc tggctacagc ctttcttacg acgtcggaaa cgaactcgta
     1801 tctcttatcc agaatcacca tttcaggttc catacccgac gtagcaagga tctcgtcagc
     1861 agtctttttt atttcggcga gaacttttcc tttgaagtta tcattgtcat gatacttctc
     1921 gattatcccc tcatcgtttt ccaaaagctt tatggccagc catccggaag gatattccct
     1981 gtccgtttga tgtttttcga taagatcctt taagagggct accttcgcat ctatctcttt
     2041 accataactg atacttctct tatggttgct cccgcccatg gcatccaccg tcttttgcat
     2101 caactcatcg atccccctac tcctagacgc aatagtggga acgaccggga cgcctatctc
     2161 ctcggataat ctttcatagt ccactttaat gtgcttcttc tccgcatcgt ctaccatatt
     2221 tagtgctata attatattgg cacccatctc catgaggagg gtcgtcagat agagattcct
     2281 ttcgaggttg gtagcgtcta ccacatttat aataatatca tgattgcctt ttaatatata
     2341 atccctggct acggcctcgt cctcggagta ccccccc
//
LOCUS       DASRNX010000174        11929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_777228,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000174 DASRNX010000000
VERSION     DASRNX010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11929
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777228"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..795)
                     /gene="rsmG"
                     /locus_tag="VFD89_06905"
     CDS             complement(73..795)
                     /gene="rsmG"
                     /locus_tag="VFD89_06905"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZJ57953.1"
                     /translation="MDHNLRKLELGMEKLGIAVTGDQIEKFDRYISLLIEWNKKMNLT
                     AITDPNKIIIDHFLDSIALLKDICLKEHNRIIDVGTGAGFPGVPIKIMKPKIELVLLD
                     SLRKRTEFLSALRDELKLSGIEIIHSRAEDLARKTEYREQFEIVLSRAVAPLNILTEY
                     CMPFVKPGGFFVSYKGPAAQDELIMAQDAIKVLGGNNSCGIKDAYVPFSDKSRNLIII
                     EKVALTPKKFPRSPGKIKKSPL"
     gene            complement(801..2678)
                     /gene="mnmG"
                     /locus_tag="VFD89_06910"
     CDS             complement(801..2678)
                     /gene="mnmG"
                     /locus_tag="VFD89_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZJ57954.1"
                     /translation="MELFAGEYEVIVIGGGHAGCEAALAASRMGYSTAIFATNLDNIA
                     LLACNPSIGGTSKGHLVREVDALGGQMGLNIDDSFIQIRMLNTGKGPAVHSLRAQADK
                     NDYHRIMKATLENQPGLSVIQGEITEIKVKSGRVVGVVTDLGDYFACRSAVVATGVYL
                     KSRIIIGEYSVNSGPSGFFPANSLSNSLKDLGFTLQRFKTGTPARIDGATINYDRMHV
                     QPGDEVIIPFSFLNDTLAREQIPCWLTYTNEKTHDIIRANLHRAPMYSGEIEGIGARY
                     CPSVEDKVVRFADKKSHQIFIEPEGRHTNEMYPQGLSTSMPMDIQRAMYRTVPGLENV
                     RIMRPAYAIEYDCIDSKELKLSLESKSIEGLFFAGQINGSSGYEEAAGQGLIAGINAV
                     QYIRDKEPVILDRSQGYIGVLVDDLVTKGTDEPYRMMTSRAEYRLLLRQDNADMRLTK
                     IGYDVGLVDENRYDWFMRKYERVEGEVNRLKDLIISPTREVLEYLEGRGSSAIKSGIS
                     LYELLKRPEIAYKDIESLGGEAEGLLRQDKEQVEVSIKYEGYIDKQLRQAQQFKRLES
                     KRIPVGIDYTNISGIRLEARQKLNLVRPESIGQASRISGVSPADISVLMVFLEKRRRE
                     N"
     gene            complement(2687..4069)
                     /gene="mnmE"
                     /locus_tag="VFD89_06915"
     CDS             complement(2687..4069)
                     /gene="mnmE"
                     /locus_tag="VFD89_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZJ57955.1"
                     /translation="MLFEETIAAISTPPGQGGIGIVRISGPKSLGIAREIFVFPGDRV
                     YPFRDRYLHYGKVRDNENRIIDEVLLTYMKAPRSYTAEDVVEIHCHGGIISVKRILEE
                     VIKNGARPAQPGEFTKRAFLNGRIDLAQAEAVMDLISAKTERSARVSVDQMGGGLSGR
                     IRDIRERLLNMMAHVEVTIDYPEEDIDEPMISRMREETTEISKEIEGLLKTAEEGKII
                     RQGIKAVIVGRTNVGKSSLLNALLRENRAIVTDIPGTTRDVIEDYINIRGIAVRIVDT
                     AGIRETPDQIEMMGIERSKKNMDDADLVIMVLDSSAPLGYDDRQIMKYLRDKKSLVLL
                     NKMDKEPLLTREDVAVELGGDIPIVNTSMTLGTGIDKVEDMIYKMFFEGEIESGHDVT
                     VTNFRHQDALIRAHNHLADIIEGLDTQIPLDIVSIDLRGALDSLGSITGETVADDLIH
                     RIFSEFCLGK"
     gene            complement(4285..4902)
                     /gene="jag"
                     /locus_tag="VFD89_06920"
     CDS             complement(4285..4902)
                     /gene="jag"
                     /locus_tag="VFD89_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HZJ57956.1"
                     /translation="MKIVESSGKTIDEAILMAVAQLGVQRDNLDIEILEEPVKGLFGL
                     IGGKDAKIRASIKQTMGDRTKGFVTDLLTYMDVEAIVETNETNEVIDVELRGRNMGLL
                     IGHRGETLDALQYLTSLVVNKEGQDYKRIALDTENYRQKREDTLRRLAKRLGYKVSKT
                     RRKITLEPMNPFERRIIHATLQNDGYVRTYSEGDEPYRRVVITCK"
     gene            complement(4899..5888)
                     /locus_tag="VFD89_06925"
     CDS             complement(4899..5888)
                     /locus_tag="VFD89_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HZJ57957.1"
                     /translation="MWQSLVGFLQTIITGINSVVGNFGISVILFTILTRLALLPLDLK
                     AKASTKKMGELNPEIQKINEKYKNDPDKKNKKTMELYQKHGVNPMGGCLPMLLQLPLM
                     FAMFAALRAISSAQLEGFYVDLIRFKDSAIGPVLDNFVKGIENSAAIKQNVGDVLTSL
                     FSNPNNERLITQLGEVVGKTDMTTIVDAIKNISTPQALDFLKTSESSLRFLWIKNVWI
                     ADSPLRDIIGRTIPFMEGGWFGAWNGLFVLSGLAGITSYLQMKLTSPSGGDNQQTKGM
                     GAIMPIMSVWFTSMYTSAFSIYWVTSNIFQIVQQLIYNRIEPGAKTGKEGAEK"
     gene            complement(6069..6278)
                     /gene="yidD"
                     /locus_tag="VFD89_06930"
     CDS             complement(6069..6278)
                     /gene="yidD"
                     /locus_tag="VFD89_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZJ57958.1"
                     /translation="MKKILIGGIRLYQRFISPLLPGSCRFYPTCSHYAILSLEKYGVL
                     RGSWKAIKRISKCHPFHPGGYDPLV"
     gene            complement(6275..6628)
                     /gene="rnpA"
                     /locus_tag="VFD89_06935"
     CDS             complement(6275..6628)
                     /gene="rnpA"
                     /locus_tag="VFD89_06935"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZJ57959.1"
                     /translation="MNKKYRLRKRKEFSYTYKRGKSLANSCLVLIYRRNGLDVSRVGF
                     SVSKKYGNAVARNRIKRRLKEIYRHRISVIKPGYDLIFIVRIGTRGASFKRLENQLVN
                     LLKRAVLFIDNGQIK"
     gene            complement(6781..6915)
                     /gene="rpmH"
                     /locus_tag="VFD89_06940"
     CDS             complement(6781..6915)
                     /gene="rpmH"
                     /locus_tag="VFD89_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZJ57960.1"
                     /translation="MKRTYQPKNRQRKKEHGFRKRMSTKNGRAVLRRRRLKGRKRLSA
                     "
     gene            7791..9122
                     /gene="dnaA"
                     /locus_tag="VFD89_06945"
     CDS             7791..9122
                     /gene="dnaA"
                     /locus_tag="VFD89_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZJ57961.1"
                     /translation="MLQKIKKVWEETLPFIKLELTEVSFNTWIKTIEPLTIIDNNFYL
                     MVPNDFTKSILESRYMDLLVNALKEVSSADYTVILALPSEANRYLNAGKDEKDSSETD
                     RGALNAKYTFDSFVIGNSNRFAHAACLAVAEAPANAYNPLFIYGGVGLGKTHLMHAIG
                     HFILSQTPHTKVLYVSSEKFTNELITSIQTNKNVEFRNRYRNVDVLLVDDIQFIAGKE
                     STQEEFFHTFNTLHESNKQIIISSDRPPKEIPTLEDRLRSRFEWGLIADIQPPDLETR
                     IAILRKKAEQEEIQVDDDILLFIAQRIQSNIRELEGALIRILAYSTLNNNRLDVDIAD
                     EALKDIISNNRPRAITPPLIMEIVSSFYNIRMDDFKSKKRNRAISFPRQIAMYLCREL
                     TDLSLPKIGEEFGGRDHTTVIHAYDKIANDIANNQELSKTVTQIRKELGES"
     gene            9335..10435
                     /gene="dnaN"
                     /locus_tag="VFD89_06950"
     CDS             9335..10435
                     /gene="dnaN"
                     /locus_tag="VFD89_06950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZJ57962.1"
                     /translation="MKFTCMQKKLSDSISIVQKAISPRTTHPILEGIFLKAYKGILKL
                     IGNDLDLGIECFLNANIHTEGSVVIPSRLFGDIIRRLPDCQIEIEVTDNYLVCIRAEN
                     SLFEIQGIKPDEYPDLENIHENDPIEINQALLKEMIQTTIFAAAVDETRPILTGALIE
                     IGNGNINMVCLDGYRLAVRQGSIDNGKEVNVIIPGKALDEVSRIIDNSDEKINITIDE
                     KHVLFDMGYTRIISRLLSGEFLNYNQIIPQEYKTRIKFDTRTLYNSIERASILAREGK
                     NNLIKLSIKEDKLVINSNSEIGKAHEEVPISMEGEEMTIAFNARYFMDILKGIGGEDS
                     YIDFTTSISPCVFRPLQGGNFTYLLLPVRINV"
     gene            10486..10704
                     /locus_tag="VFD89_06955"
     CDS             10486..10704
                     /locus_tag="VFD89_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZJ57963.1"
                     /translation="MENIVISTEFIKLSQLLKWAGIADTGSRANDMIAEGLIKLNGIP
                     ETRRGKKIYHGDKILLEDKYEFLVIREG"
     gene            10711..11820
                     /gene="recF"
                     /locus_tag="VFD89_06960"
     CDS             10711..11820
                     /gene="recF"
                     /locus_tag="VFD89_06960"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HZJ57964.1"
                     /translation="MYLRELGLTNYRNYSKLELDFNSNLIIFSGRNAQGKTNILESIF
                     LSCTGRSHRTPRDKELILQGEKEAAIRTMVQKREGPSLIEITLGVKENKKIHINGMAA
                     QRLGELMGHINSVLFSPEDLKLVKGGPAERRRFMDMEISQIKPQYFYSLQQYNRILSH
                     RNNLLKDINENSSLKSTLPVWDEQIARVGTYIIAERLDFTENIGKIAHNIHRQISSSG
                     ENLEVLYDPSIRFDSPHKNEIEECYLRTLESNHEEDIRRGRTGKGCHRDDLIIGIDDM
                     DVRTFGSQGQQRTSVLSLKLSEIEMMFRETGEYPLLLLDDVLSELDRYRQEQLLSYFD
                     KLQTFMTLTDYPDILGDMGNDSEIYEVLKGRVTKI"
BASE COUNT         3373 a         2743 c         2255 g         3558 t
ORIGIN      
        1 agtatatata ggttttgctg gatattactc catattgcga ctggttttac tatattttca
       61 caaacaaagt cattataacg gtgatttctt tatttttccc ggcgaacggg ggaatttttt
      121 aggcgttaat gctacttttt caattataat aagattgcgg cttttatccg agaacggaac
      181 atacgcgtcc ttgatgccgc aggaattatt tccgcccaat actttgatag catcttgggc
      241 cattatcagc tcatcctgtg cggcaggtcc tttataggat acgaaaaacc cacccggctt
      301 tacaaaaggc atacagtatt ccgttaggat attcaaagga gcaaccgccc ttgagaggac
      361 gatttcaaat tgctccctgt attcggtttt tctcgctaaa tcctccgccc tcgaatgaat
      421 aatttcgata cccgacaatt tcaattcgtc ccttaaggcg gataaaaatt cggtcctttt
      481 cctcaatgag tccagaagaa ccagttcgat cttaggtttc attatcttaa tgggcactcc
      541 gggaaagcct gcaccggtcc ccacatcaat tatccggtta tgctccttta gacatatatc
      601 cttcaataag gcgatggaat ctaagaaatg gtctattata attttattag gatcagttat
      661 tgctgtcaaa ttcattttct tattccactc tattaatagg gaaatatacc tatcaaactt
      721 ttcaatctga tcccctgtaa cggctatacc cagtttttcc atgcccagtt ccagcttgcg
      781 tagattgtga tccatatccc ttaattctct ctccttctct tttccaaaaa gaccattaag
      841 accgaaatat ccgctgggga taccccagaa attctactgg cctgtcctat tgactctggc
      901 cttactaaat tcaatttttg tcttgcctca agcctgatcc cggaaatatt agtataatca
      961 atacccacag gtattctttt agactccagt cttttaaatt gttgagcctg ccttaactgc
     1021 ttgtctatat agccctcgta ctttatagat acttcaacct gctctttgtc ctgccttaag
     1081 agcccctcag cctcgccacc caggctctct atatccttat atgctatctc cggcctcttt
     1141 aataattcat agaggctaat accacttttg atagccgacg atccccttcc ctcgaggtat
     1201 tctaatactt ccctcgtagg ggaaataata agatccttaa gtctgttaac ttccccttcc
     1261 accctttcat acttcctcat aaaccaatca tagcgattct cgtctaccag gcccacatcg
     1321 tagccgatct tggtcagcct catatccgcg ttgtcttgcc tgagcaataa tcgatactcg
     1381 gctcttgagg tcatcatcct gtagggttca tcggttccct tagtcactaa atcgtctacc
     1441 aatacaccaa tatagccctg ggacctgtcc aatattacag gctctttatc tctaatgtat
     1501 tggaccgcat ttataccagc tataagcccc tgtcccgcgg cttcttcata tcccgaactg
     1561 ccgtttatct gtccggcaaa aaaaagtcct tctatggatt tgctctctaa agatagcttt
     1621 agttcctttg aatctatgca atcatattcg attgcataag ccggccgcat tattctcaca
     1681 ttttccaaac cggggacagt cctatacatg gccctttgta tatccatagg catactggta
     1741 gacaacccct gaggatacat ttcattggta tgccttccct ccggttcgat aaatatctga
     1801 tggctcttct tgtctgcaaa tctgaccacc ttatcctcca cagacggaca atatctggcc
     1861 ccaattccct caatttcacc cgaatacata ggcgccctgt gaaggttggc tctgatgatg
     1921 tcatgggtct tttcgttagt atacgtaagc cagcatggaa tctgttccct ggctaatgta
     1981 tcgttcaaaa aggaaaaggg tattatcacc tcatcccccg gctgaacatg catcctgtca
     2041 taatttatgg ttgccccgtc tatccttgcc ggggtacccg tcttgaatct ctgcagggta
     2101 aagcccaaat cctttaggct attcgaaagt gaattggcag gaaaaaaacc actgggtcca
     2161 ctatttacgc tgtattcgcc tattattatc ctgctcttta agtatactcc cgtcgccaca
     2221 accgctgatc tgcaagcaaa ataatcgcca agatcggtta ccacccctac tacccttccg
     2281 ctctttacct ttatctcggt aatctccccc tgtataacgc taaggccggg ttggttttcc
     2341 aaggtagctt tcatgatccg gtggtagtca ttcttatcgg cctgagccct gagggaatgt
     2401 acagccggtc ccttacccgt gttcaacatc cttatctgta tgaaggaatc gtctatatta
     2461 aggcccatct gcccccccag tgcatctact tcccggacga ggtggccctt tgatgtgcct
     2521 ccaatggagg gattgcaagc taacagggca atattatcca ggttagtcgc aaagatagcg
     2581 gtactatacc ccattctaga cgcagccaag gcagcctcac atcccgcatg gccccctcca
     2641 atcactatca cttcgtattc cccggcaaag agctccatac attctcctac tttcctaaac
     2701 aaaactcaga aaatattcta tggatcagat catcggcaac tgtttcacct gtaatgctcc
     2761 ccaatgagtc caaagcaccg cgcagatcga tagaaactat atccagaggg atttgggtgt
     2821 ccagcccctc tataatgtct gccaggtggt tatgagccct tatcaaggca tcttgatgcc
     2881 gaaaattggt taccgtaaca tcatgacctg attctatttc cccttcgaaa aacattttat
     2941 atatcatatc ctcaactttg tcaataccgg ttcccaaggt catggaagtg ttgactatgg
     3001 gtatatcgcc gcccaattca actgctacgt cttccctggt caataggggc tccttgtcca
     3061 tcttatttag taggaccaga gactttttat cccttagata cttcattatc tgcctgtcgt
     3121 cataccctag gggtgccgag gagtctaaga ccattatgac gagatccgca tcatccatgt
     3181 tctttttaga tctctcgata cccatcatct ctatttgatc cggggtttcc ctaattcccg
     3241 cggtatccac tatccttacg gctattcccc ttatgtttat ataatcctct attacatccc
     3301 tcgtagtccc cggtatatcc gtaactatgg cgcggttttc cctgagtaac gcattcaata
     3361 gggacgactt gccgacattg gtcctcccga ctataaccgc cttaatcccc tgccttatga
     3421 ttttaccttc ctcggctgtc tttagcaatc cctctatctc tttggagatt tccgtggtct
     3481 cctccctcat gcgggaaatc atcggctcat cgatatcttc ttcgggataa tcgatggtca
     3541 cctccacatg agccatcata ttcaggagtc tctcccttat atcccttatt ctcccggaga
     3601 gccctccgcc catctggtct acggataccc tggcggatct ttccgtcttg gccgaaataa
     3661 ggtccataac cgcctccgcc tgtgctaaat cgatcctgcc gtttaaaaat gccctttttg
     3721 taaattcccc cggctgagct ggtctggccc cgtttttaat tacttcttcc aagatcctct
     3781 taaccgatat aatacccccg tggcaatgga tttctactac atcctcggcg gtgtaggacc
     3841 ttggggcctt catataggta agaaggacct cgtctataat cctattttca ttgtccctta
     3901 ccttcccgta atgtaagtat ctatccctga aaggataaac cctatcgccc ggaaaaacaa
     3961 atatttccct tgcaatgccc aaggattttg ggccgctgat ccttactatc cctataccgc
     4021 cctgacccgg cggagtcgaa atagcagcta tggtctcttc aaaaagcata tctatccttc
     4081 ccctttttag gaaacaaaaa agtttaaccc agtatagtta tactgggcta ttttctagcg
     4141 gattaaacaa ttcaaatgtt acaaaaatgc aatttatatc acaaaatcgg atgataaagc
     4201 ctattctgca atgaaaagcc tttacgcaac atatatagtc ctactaagtc tcgcggataa
     4261 taatcctaca acgcaatctc gggtctattt gcaggttatt acaacccttc tgtagggctc
     4321 gtccccctcg ctgtaggtcc ttacatagcc atcattttgg agggtcgcgt ggataattct
     4381 tctctcgaat gggttcatag gctctaaggt gatcttccgg cgagtcttag aaactttgta
     4441 gcccagcctt ttggccaaac gcctgagggt atcctccctc ttttgtctgt agttttccgt
     4501 atccaaggca atccttttat aatcttgccc ttccttattg actaccaggc ttgtcagata
     4561 ttgaagagca tccaaagtct cacccctatg gccgatcaat aagcccatgt ttcgacctct
     4621 cagctctacg tcaatgactt cgtttgtttc gtttgtttcg actattgctt caacatccat
     4681 ataggttaaa agatccgtga caaaaccctt cgtcctgtcg cccatagttt gctttatact
     4741 tgcgcgtatt ttggcatcct ttcccccaat aagcccaaac aaacccttta ccggttcttc
     4801 caatatttca atatccaggt tatccctctg tacccctaat tgtgcaaccg ccatcaaaat
     4861 agcttcatca atagtctttc cactactctc aacgattttc atttctctgc accttccttg
     4921 cctgtttttg ctccaggttc tatccggtta tagatcagtt gttgtacgat ctggaatatg
     4981 ttactggtta cccaatatat gctgaaagct gatgtataca tcgaagtaaa ccaaaccgac
     5041 ataatgggca taatagcccc cattcctttt gtctgctggt tatcaccgcc tgatgggctg
     5101 gtcaatttca tttgaagata ggatgtaatt cccgccaatc ccgataaaac aaataatccg
     5161 ttccatgcac caaaccatcc gccttccata aatggtatgg tcctgcctat tatatccctc
     5221 aagggagagt ccgctatcca tacattttta atccaaagaa acctgagact tgattcggaa
     5281 gttttaagaa aatccaaagc ctggggtgta cttatatttt taatggcatc tacaatcgtt
     5341 gtcatatcgg ttttccccac aacttcgccc aattgtgtaa tcagccgttc attgtttgga
     5401 ttgctaaaga gactggtaag cacatcaccc acattttgtt ttatggctgc ggaattctct
     5461 atacccttta caaaattatc aaggacaggg ccgatggcgc tatctttaaa ccttataaga
     5521 tcgacataaa aaccttccaa ctgtgcgctg gatatagccc taagcgccgc aaacatcgca
     5581 aacataagag gcagttgtaa taacatgggg agacatccgc ccatgggatt gactccatgc
     5641 ttctgatata gctccatagt cttcttgttc tttttatcag ggtcattttt atatttttca
     5701 tttatctttt gaatttccgg gttcaattcg cccatttttt tagttgaagc cttggctttt
     5761 aggtcgagag gcagcagcgc caaacgagtt agtatagtaa aaaggatcac tgatatgcca
     5821 aaattgccga cgacgctgtt tatacccgtg attattgttt gaagaaaacc aaccagggat
     5881 tgccacatat tataacaccc gcctcttcac ctgttttatg gatttgtaat ctattaattc
     5941 cattcctatt tttacctccg gaacatattt gtccaataat gaacctttaa atatcggaag
     6001 caatcgctaa agcaattcca acacaccgtt tattgaagtg aggggcgcat ttcaccctgc
     6061 tataattatc acaccagggg atcatatcca ccgggatgaa atgggtgaca cttggatatc
     6121 cttttaattg ccttccacga acctcgcaaa accccgtatt tttccaacga taatatagcg
     6181 taatgagaac aggtgggata aaatctgcag ctacccggga gcaacggcga tataaacctt
     6241 tgatataatc taattccacc tatcaaaatc tttttcattt aatctgtccg ttatcgataa
     6301 ataatactgc ccttttcaat aaatttacca attgattttc caatctttta aagcttgctc
     6361 cccttgtccc aattcttaca ataaagataa gatcataacc tggcttaatc acgcttattc
     6421 tatgtctata tatttctttt agccttctct tgatcctatt tcttgctaca gcgttcccat
     6481 atttcttgct aacggaaaat cccaccctgg aaacgtccag tccgttcctt ctgtatatca
     6541 ataccaaaca agaattcgca agggatttcc ctctcttata ggtgtaactg aactcttttc
     6601 gctttctcag ccgatatttc ttgttcacaa aataccctct cgctatgaat tattttctca
     6661 gtatttgtcc caagacagac aaataggtta caccaagtcc caagagaaaa atcgcatcgg
     6721 gccactaagg attatcccaa gatttccaat aaggcagaag aagccaccgc ggaagtggcc
     6781 ttaagctgat aatctctttc tacctttaag tctccttctt ctaagcaccg ccctgccatt
     6841 tttggtgctc attctcttcc taaaaccatg ttcctttttc ctctgtctat tcttaggttg
     6901 atatgttctc ttcattccgg tatcccccct tttatatgct tttgccaagg agtacagatc
     6961 taattccatg gggcaaaagg catttccatg attcatatgc tatgaaaagt acattttttt
     7021 gactagtctt cttcttaaca tatggagcca tccatcaaat aggcccgaat caattttgta
     7081 acaatatcca aaatctttcc tatttggccg atgtcggcac tttactatag tattataagc
     7141 gaatagccct tttgctgtca agaaaatcaa caaattcaat tgattatcca cagattattt
     7201 gatattatat ctttactgtt gataacttcc tatttataat agaagggaat ggttataagt
     7261 tatacacatt ctgtggataa ctttgtgaat aactttttac gcctttcact ttttggttaa
     7321 agggccagtt ttttttggtt gttagtattg tttataactt ttacattttt gtgggatatt
     7381 atgcctggtc ttatatataa tttgattagg gtttaggttt tggtaaaacg ccccgatttc
     7441 cgggacgatt ctattacgat ggactctgtg tacatacttt agcttatttt agtatccatg
     7501 aatccaaaat tataatacta acccgaaggg tttaaccgtc tacagggttt taaaaagaat
     7561 aaattggtaa aaatatgtat taaaacatcg gaagaaattg cattggcaat tgcaacacac
     7621 aggtttcaac gaggcggggg atttataccc accacctgtt ttactaatcg gaactcgccg
     7681 cttatagaag cgggagacat cttaccgact cgttataacg agagggtata atgcgattac
     7741 cgaggtaatt gccccgatgt tataacggta tcttatggga ggtttccttg atgttacaaa
     7801 aaattaaaaa ggtatgggaa gaaacgctgc cttttattaa gctggaacta actgaggtca
     7861 gtttcaatac ttggattaaa acaatagagc ctttgacaat catcgacaat aacttttatc
     7921 ttatggtccc caacgatttt acaaaaagca ttttagaatc aagatatatg gatctgctag
     7981 tcaacgcctt gaaggaggtt tcatccgcag attataccgt tattctcgcc ttacccagtg
     8041 aggcaaacag gtatctcaat gccggcaaag acgagaagga cagttccgag actgacagag
     8101 gtgccctaaa tgcaaaatac acctttgatt catttgttat cggcaacagc aatagatttg
     8161 cccatgctgc ctgtcttgcc gtagccgaag ctccggctaa cgcctataat cccttgttta
     8221 tatatggggg agtcggactt gggaagaccc atttaatgca tgctatcgga cactttattc
     8281 tttcccagac cccgcatact aaggtattat acgtttcgtc agaaaaattc accaacgagc
     8341 ttattacatc cattcagacc aacaagaacg ttgaatttcg gaacaggtat cgcaatgttg
     8401 atgtgctttt agtggacgat attcagttta ttgcaggcaa ggagagtacc caggaggagt
     8461 ttttccatac cttcaatacc ctccatgaat ccaataaaca gattatcata tccagcgacc
     8521 gacccccaaa ggagataccc accctagagg acaggcttcg ttccaggttt gagtggggcc
     8581 ttatagccga tatccaaccg ccggatcttg aaaccagaat agctatacta agaaagaagg
     8641 cagaacagga agaaatccaa gtggatgatg atattctttt atttattgcg cagcgtatcc
     8701 agtccaatat cagagaattg gaaggtgccc tcattcgaat tctggcttat tccaccctca
     8761 acaataacag gttggatgtt gatatagccg atgaggcact taaggatatt atctccaata
     8821 accgtccaag ggctattacg cccccattaa taatggagat cgtttcgagt ttttacaata
     8881 taaggatgga cgattttaaa tctaaaaaga gaaatagggc catttcattt ccccgtcaaa
     8941 tagcaatgta cctatgtagg gaacttactg atctttccct gcccaagata ggagaagaat
     9001 ttggggggcg ggatcatact accgtcatcc atgcctatga caaaattgct aacgatatag
     9061 ccaacaatca ggagttatca aagacggtaa cccagattcg aaaggagttg ggggaatcat
     9121 aacccgtata ttacgattat ttagcaattt tagctaacag gtcgtagtgc tgatttgtgg
     9181 ataactttta aaaaaacttt tattcttcca cacacttatc cacatacctt tttcgaggtt
     9241 tatgcgatta tttgacataa ttcacataat cacagcccct actactatta ctactaaatt
     9301 attatatata ttcaacatac tcaggaggta tgtcatgaaa tttacatgta tgcaaaaaaa
     9361 attatctgat agcataagta tagttcaaaa agccattagc cccaggacca ctcatcccat
     9421 tcttgaggga atctttttaa aagcatataa gggaatcctc aagctcatcg gcaatgattt
     9481 ggatttgggt atagaatgct tcttgaatgc caacatacat acagaaggat ctgtcgttat
     9541 accctctcgg ttgtttggcg atataatcag aaggttaccc gattgccaaa ttgaaataga
     9601 ggtcacggat aattatttgg tctgcataag agcggaaaat tccctctttg aaatccaggg
     9661 aataaaaccc gacgaatatc cggatcttga gaatatccat gaaaatgatc ccattgagat
     9721 taatcaggct ctccttaaag agatgatcca aacaactatc tttgcagcag ctgtggacga
     9781 aacgaggccg atcctaactg gagcccttat agagattggc aacggcaata tcaatatggt
     9841 atgcttagac ggctatcgat tagccgtgag acaggggagc attgacaatg gaaaggaagt
     9901 aaatgtaata ataccgggca aagccctaga tgaggtcagc aggatcatag acaacagcga
     9961 tgagaagatc aatattacca tagatgagaa gcatgtactc tttgatatgg gttatactcg
    10021 aattatttcc aggttgctca gcggggaatt cctcaactat aaccaaataa tcccccagga
    10081 atataagacg cgaataaagt ttgacaccag aaccctctat aattccatag aaagagcctc
    10141 tatactggca agggaaggaa agaacaacct cataaagctg agcataaaag aggataaact
    10201 ggttataaac tccaactcgg agataggaaa agcccatgaa gaggtgccca tctccatgga
    10261 gggggaggaa atgaccattg ccttcaatgc cagatacttc atggatattt taaagggaat
    10321 aggcggggaa gattcatata tcgactttac taccagtata agcccctgtg ttttcagacc
    10381 cttacagggc ggcaacttca cctatctttt attgccggtg aggattaacg tataattttt
    10441 tttggtaatg gtttaatttg atgcgttacg atggaggaaa tatcattgga aaatattgtt
    10501 ataagtacag agtttataaa gctaagccag ctcttaaaat gggccgggat tgccgatacc
    10561 ggttcccgtg ccaacgatat gatagccgaa ggtctgatta agttaaatgg cataccggag
    10621 accagaaggg gaaaaaagat atatcatggg gataaaattc tattggagga caagtacgaa
    10681 ttcctggtca taagggaagg atgaaggaaa ttgtatctga gggaattggg tctgacaaat
    10741 tatagaaact atagtaaatt agagttggat ttcaactcta atttaattat tttctcgggt
    10801 agaaatgccc agggtaaaac caatatccta gagtctatct ttttgtcatg tacgggcaga
    10861 tcccatagaa cccccaggga caaagagctg attttgcagg gtgaaaagga ggccgccata
    10921 agaacaatgg tccaaaaaag ggagggaccg agcctcattg agattaccct aggcgttaaa
    10981 gaaaataaaa aaattcatat aaacggtatg gctgcccaga gactgggtga gcttatgggg
    11041 catataaaca gcgtcctttt ctcacctgag gatttgaagc tggttaaggg cgggcctgcc
    11101 gagcggagaa gatttatgga tatggaaata tcccaaataa agccgcaata tttctatagt
    11161 ctccagcaat ataacagaat cctaagccat agaaacaacc ttctaaagga tatcaatgaa
    11221 aatagtagtc taaagagcac cttacccgta tgggatgagc aaattgctag ggtgggaacc
    11281 tatataatag cggagagact ggactttact gagaacatcg gaaagattgc ccataatata
    11341 cacaggcaaa tatcctccag cggtgaaaat ttagaggtgc tatacgatcc ctccattagg
    11401 tttgattcgc cccataagaa tgaaatagag gaatgctatt taaggaccct agaatcaaat
    11461 catgaagagg atataaggag ggggaggacc ggaaagggat gccataggga cgatttaatc
    11521 ataggcatag atgatatgga tgtgcgaact tttggttcac agggacaaca aaggaccagc
    11581 gtattatccc ttaaattgtc ggaaattgag atgatgtttc gggaaaccgg cgaatatcca
    11641 ctcctgcttt tagatgatgt cctatcagaa ctggatagat atagacaaga gcaactgctt
    11701 tcatactttg ataagttaca aacctttatg actttgacgg attacccgga tatattggga
    11761 gatatgggaa atgattcgga gatatacgag gtattaaagg gcagagtcac gaagatttaa
    11821 tttttcttta agtataggat aaaataggat ataatagaga tgttaaggag tcataaaaca
    11881 aacaacccat cggtgggggt ttcaaaaata aattattgtc attataacc
//
LOCUS       DASRNX010000175        11277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_354068,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000175 DASRNX010000000
VERSION     DASRNX010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11277
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354068"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1037
                     /locus_tag="VFD89_06965"
     CDS             42..1037
                     /locus_tag="VFD89_06965"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZJ57965.1"
                     /translation="MKLLISGYGGGKIDSIGMYDVGAEGRDKKLLWSSNIENPSYLDY
                     HDNMLFGLTEKGRGNNVCLFLEEEGGYRLADSRDLEGNGLCHITYLPNNRALVGSCYG
                     SGDIFSIGVEDQAFGELHSMLRQGEEAKVTTRAHSAVFDPDEKRVYSANIALDRIYVY
                     GMDQGALKEIDYFQLDEGEGPRHIALYPELNLIYIITEYSNRIIILKSEESGFKMIQS
                     ISTLPEDFEGKSFCSTLCFTKDRRFLYGANRGANTIGIFKVKEDGTLEKIGDSSCFGD
                     WPRHMDLVGNDKFIAIANERSGEVVIAERDEETGLIGEEINRIEFGRPSFVVEKR"
     gene            1334..1699
                     /gene="acpS"
                     /locus_tag="VFD89_06970"
     CDS             1334..1699
                     /gene="acpS"
                     /locus_tag="VFD89_06970"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZJ57966.1"
                     /translation="MVLGIGIDIIEIHRIAKAMDNPVFMDKIYTLREQEYFRTRNNNP
                     STAAGYFAAKEAAAKALGTGFGRVKWTEIEITRDEYGKPCMKLYGNAKSVMESMGGST
                     VLVSISHSRDNAISEVLIC"
     gene            1712..3313
                     /locus_tag="VFD89_06975"
     CDS             1712..3313
                     /locus_tag="VFD89_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZJ57967.1"
                     /translation="MYVVTPEEMRKIDNRAIEEFGIPGIVLMENAAIRTVDVVEREYP
                     NLYNCGRILVLVGGGNNGGDGLAIARHLKLRGADVKAALFVDGDKLAGDAKINYDIYR
                     ALDGEILTITRENPNRLGELDSYIGWADLVIDGILGTGITRDIEGIIFDAIKRVNISP
                     APVVAVDIPSGINGITGQVMGIAVRADYTVTFGNIKRGHLFYPGREYSGKLFVSPISL
                     PQCSAGATGVKAFTLDDGEVSGFLKGRPKDGHKGTFGKVGIIAGSLGMAGAACLTSMA
                     ALKSGAGLVTLGSPSSLVPIFQSKMTEVMTYPLEDGEEGYLTKDAIPSVGNFLKDKDV
                     LAIGPGLGPKCHGLEILRYILGEFDISIIMDADALNHISGDTGLLSAYKGSAVITPHP
                     GEMSRLTGLSIDEITKSPIDAATDFARETDVVVLLKGATTVVAHPDGRIYLNTSGNSG
                     MGTGGSGDILTGMIAALAAQGYSAFDAAVYGCYIHGRAGDYAMDEWGETGLVAGDILG
                     MVPLVFKDMYALKKVRPGRCNKEPI"
     gene            3469..4086
                     /locus_tag="VFD89_06980"
     CDS             3469..4086
                     /locus_tag="VFD89_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57968.1"
                     /translation="MKAFKFALILGWVLCFVGCKGRTPEDTFIAAQKALISLESYSAT
                     LTYKVMDDEDGREYRFNQWVSMPHSFKIQLIAPDNLVGKTIISNGNEILIEHSKVEDS
                     IKFNVKSLEQQRPLFIGDFLNSFLLSEEVDKRVHTEDDIEYVILACPSMNNKVLGDTQ
                     ELWLKAPKMIPVKLVTYDADGGISSLILFEEFDRSWKPEENFFDL"
     gene            4247..4522
                     /locus_tag="VFD89_06985"
     CDS             4247..4522
                     /locus_tag="VFD89_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HZJ57969.1"
                     /translation="MAELKKILVSLPDSLLREVDEIVAMGKKNRSEFIREAMRLYIRE
                     RQKIEIREKMKKGYEEMARINSELTEEGANSDIEALERYEAVLTGRE"
     gene            4526..4873
                     /locus_tag="VFD89_06990"
     CDS             4526..4873
                     /locus_tag="VFD89_06990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZJ57970.1"
                     /translation="MIKRGDIYYADLSPVVGSEQGGVRPVLVIQNDIGNRYSPTIIVA
                     AITSQIHKGKLPTHIEISASDYGLPRDSVILLEQIRTIDKRRLKEKIGFLAPETMGKV
                     DESLQISFGLIDI"
     gene            4936..5778
                     /locus_tag="VFD89_06995"
     CDS             4936..5778
                     /locus_tag="VFD89_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HZJ57971.1"
                     /translation="MVKRSAYSDYIFITLGTLVTGLAFNLFLIPHRIAPGGISGIATI
                     IYYITKGRIPVGISMLGLNIPLFIIGIREMGRGFGLKTLISTILLSLFIDILKVPSVT
                     DEPILASIYGGVVMGVGLGMVFRGNATTGGTDLFAKIIHKHFHFIGVGWVLFVVDSLV
                     VLGAAVAFGPTLALYALVSLYLGTKLIDLILEGIHTAKAFILISDRSVEIAQRIMEDL
                     DRGVTLLQGKGAYTMDEKDVILCVTDRVQVTKLKEIIGEIDPAAFVLVADVREVMGEG
                     FSLE"
     gene            5839..6825
                     /locus_tag="VFD89_07000"
     CDS             5839..6825
                     /locus_tag="VFD89_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57972.1"
                     /translation="MEERTNVLKPMTVSEILDDTFRVYGKNLWTVLIYSAVIGGIFSL
                     IVSFITGQMMVPVDTFFKDWMNILENPNIDPETAFGPIMEMMPRFFALQGMVTLLSLI
                     GLIFVNPFIQGGIINVTYSYITGRSMSINESLISTLKKFWKLILTSLSLIPYFIGVGI
                     AFVILTVISIIPLVLSGISMSKDPTGGRIVGFIFLIFVAVALMIILAVLAGMFITFTF
                     HVATTEESYGFNAIGRSFKLVAKKFWRVLGISLLINLIVGIIAGIIALVSGITSLVTP
                     FAAISNYMTGFITSAFITPIIHISTTLLFMDTKARVEPRVHDGYYEQIQNQG"
     gene            6888..7682
                     /locus_tag="VFD89_07005"
     CDS             6888..7682
                     /locus_tag="VFD89_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZJ57973.1"
                     /translation="MDVGISTASFYPDALTEQSIPLIRGLGAEKIEVFLGSFSEYEED
                     FCNRLRDIIDENGLSVYSVHVLGTQFEPQFFSFTERQRNDSRDLFLKVFKCAKILGAQ
                     AYVFHGPPVRVRAKPDIDFKRIGPISDELAYLAGDHGLKFSWENVSWCWYSYPEFADD
                     LLEHTKGDNIFFTLDIKQAMQTGHDPMDFLERMGDRLINIHAIDFNEKRELTLPGRGI
                     FDFRSLADKLESINYGGPIFLEVYRGNYEDYSDLKESMDFLRGIFC"
     gene            complement(7979..8545)
                     /locus_tag="VFD89_07010"
     CDS             complement(7979..8545)
                     /locus_tag="VFD89_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57974.1"
                     /translation="MDAKKRPVNGLLILTIMAAAIWYGSSVQKNPYMGKQFAAFIHMG
                     QGLGDVESVETSKADKYGYIRPQVLDGFSDEPIEGAVVVIPETNASYRTDEKGYTPNI
                     KIEVVPDAHYRDINPKTWGEATLIAYKEGYVEYVLLHINVWESQDRAGPKIRLFPVEN
                     QELDQPMSIVEGPNQLWINSLVEKYRPH"
     gene            complement(8780..9637)
                     /locus_tag="VFD89_07015"
     CDS             complement(8780..9637)
                     /locus_tag="VFD89_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZJ57975.1"
                     /translation="MHRDNCDTYVGHRGRLMDRDHDLIVYHYGHEKCRGDHFWTGVRD
                     HYLIHYIISGKGVFVYNNKTYHLKTGQGFLSCPNTLSYYQAHSTEPWEYCWVGFQGRK
                     ARHHLNLLNLSNHNPIIDCRPNPSFKMIVEEMIDTKNITTGRELVLTGLLYHFFALLL
                     QNERTLHHTEESRNYSKVYVDKAIDYIEKNYSHKITIEGIADYIGIDRKYLSSLFKDI
                     RLTSPQNFLIKYRMDKACILLTDELLSITHVAHSVGYDDPLLFSKMFKKYKGMPPTQY
                     RKRVLGKLS"
     gene            9874..11196
                     /gene="melA"
                     /locus_tag="VFD89_07020"
     CDS             9874..11196
                     /gene="melA"
                     /locus_tag="VFD89_07020"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HZJ57976.1"
                     /translation="MKKIAFIGAGSFGFTRSLVRDILSFPAFRDATIALMDISEERLS
                     YIHQAVDRIIKEGEYPAKVMATTDRIEALKDADGVIITILQGGVHVWRHDVEIPKKYG
                     VDINVGDTRGPAGIFRALRTIPVMLDICKDINEYCPKAIVLNYTNPMAMLCRAMQAEY
                     PNLAISGLCHSVQGTAHMLAKWIGAPKDEITYKCAGINHQAFYLDFKWNNEDAYPLIH
                     KAITENQDIYNEEQVRNEMYLHLDYYVTESSGHNSEYNAWFRKRPDLIEKYCTHGTGW
                     NPGVYAYILDEYLAREDNWKDDIEEWLNQDEIDLERGEEYAAYIFNAIFGDGTMFEFN
                     GNVRNFGLIENLPEGCCVEVPVLASKRGLDPIHVGPLPEHLAILNNINAGCEELAVEG
                     ALEGDKRKIFHAVCLDPLTSAVLSLQEIQDMVDEMFEANKDWLPHFDI"
BASE COUNT         3100 a         2289 c         2903 g         2985 t
ORIGIN      
        1 cagcattaaa aatgctatca ttaaattagg aggttgattt tatgaaactg ttaatttcag
       61 gttatggcgg cgggaagatc gattcaatcg gtatgtatga cgtaggtgca gagggtaggg
      121 ataaaaagtt gctgtggagc agcaacatag aaaacccttc atatctcgac taccatgaca
      181 acatgctgtt tggtctcacg gaaaaagggc gcggaaacaa cgtgtgcttg ttcttagagg
      241 aggagggggg ctatagatta gcagactcca gggacttaga agggaatgga ttatgtcata
      301 tcacctacct gcccaataat agggctttgg tgggcagctg ctatggaagc ggggatatat
      361 tctccattgg agtggaggat caggcgtttg gggaacttca cagcatgctg aggcaaggtg
      421 aagaggccaa ggtaacaacc agggcccaca gcgccgtatt cgatcccgat gaaaaaaggg
      481 tttattctgc caatatagct cttgatagga tctatgtcta cggcatggat cagggagcac
      541 tcaaggaaat agactacttc cagctggatg agggcgaggg gccgagacat atagccctgt
      601 atcccgaact aaaccttatt tacataatca cagagtattc caatagaata attatcctaa
      661 aaagtgaaga gtccggcttt aagatgattc agagcatcag taccctacct gaagattttg
      721 aaggaaagag cttctgttct accctgtgtt tcacaaagga taggcgattt ttgtacgggg
      781 caaaccgcgg agccaacacc atcggaatat ttaaggtgaa agaagacggt accctagaga
      841 agataggcga cagttcctgc tttggggatt ggcccaggca tatggacctg gtgggcaacg
      901 ataaatttat tgccatagcc aacgagagaa gcggcgaagt ggtaatagcc gagagggacg
      961 aagagaccgg tctcataggc gaagagataa acaggattga atttggcaga cctagctttg
     1021 tggtggagaa gcggtagatt aacctgttta ctataactat ggggtaatga actattgaac
     1081 gctacttccg acattgattt ggagaaaact aaggcttatt ccatttgaaa agcctatcgc
     1141 ttccaatccg gcctcctgcc ggtggaatcg gtttaaggcg tcctgcctta aactacggct
     1201 tttctgcatt ccataagcca agttttctta cccaatcaat gaagtcattc gcttttcaat
     1261 agttcattac ccagatactg gattggtata atgcagtaaa ttgtaggcaa acaacgatga
     1321 aagaggtggc attttggtac tggggattgg gatagatatc atcgaaatcc acagaattgc
     1381 caaggccatg gacaacccgg tatttatgga caagatatat actcttaggg aacaggagta
     1441 tttcaggacc aggaataata atcccagcac cgcggcgggt tactttgccg caaaggaagc
     1501 cgccgcgaaa gctctgggta cgggttttgg ccgggttaaa tggacggaga tcgagataac
     1561 ccgagatgaa tatggaaaac cctgcatgaa actctatgga aatgcaaaga gtgttatgga
     1621 gagtatgggt ggaagtaccg ttttggtatc aatttcccat tccagggata atgccatttc
     1681 agaggtttta atctgttagg ggtgattgga tatgtatgtg gttacgcctg aagagatgcg
     1741 gaaaatagat aacagggcta ttgaggaatt cgggattccg ggaatagtgc ttatggagaa
     1801 tgcggcgatt agaacagtcg atgttgtgga gagggaatac ccgaacctgt acaattgcgg
     1861 caggatttta gtgctggtcg gtgggggtaa caacggcggg gatggattag ctatcgcaag
     1921 gcatttaaag ctgagagggg cagacgtcaa ggctgcccta tttgttgatg gggataaatt
     1981 agccggcgat gcgaagataa actatgatat ctacagggct ttagacggtg aaatcctcac
     2041 cattacccgg gaaaacccca atcgtttggg cgaattggac agttatatag ggtgggccga
     2101 cctggtaatc gacggcatct taggaactgg gataaccagg gatatagaag gtatcatctt
     2161 tgatgccata aaaagggtga atatatcgcc tgcaccggtg gtggctgtgg atattccctc
     2221 gggcataaac ggtataaccg gacaggtaat gggcatagcc gtcagagccg attataccgt
     2281 gacctttggc aatatcaaaa ggggacacct gttttatccc ggccgggaat attccggcaa
     2341 actctttgta tcccccataa gcctgcccca atgcagcgcc ggggccacag gggtcaaggc
     2401 ctttacctta gatgacgggg aggtatccgg gttcctgaaa ggaagaccta aagacgggca
     2461 taagggcacc ttcgggaagg tggggattat tgcgggctct ctgggtatgg caggcgctgc
     2521 gtgtcttaca tctatggctg ccctcaagag cggcgcaggg cttgttaccc tgggcagtcc
     2581 ctccagcctt gtgcccatat ttcaaagcaa gatgaccgag gtcatgactt accccttaga
     2641 agacggggag gaaggctatc tgaccaagga cgccataccc tctgtcggaa attttctaaa
     2701 ggacaaggat gtactggcca tcgggccggg gctgggacca aaatgtcatg gtttagaaat
     2761 tttaaggtat attttgggcg aatttgatat atccataata atggatgcgg acgcactaaa
     2821 tcatatatct ggggacacag gattattgtc cgcgtacaaa gggtctgcag tcataactcc
     2881 ccaccccggg gaaatgagca gattgacggg gttgagcata gatgagatta ctaaatcccc
     2941 catagatgcg gctaccgatt ttgcaaggga gacggatgta gttgtactgc tgaagggtgc
     3001 gactaccgta gtggcccatc ctgacgggag aatatattta aatacctcag ggaattccgg
     3061 aatggggacc ggcggaagcg gggatatcct tacggggatg atagccgcac tggccgccca
     3121 gggctattct gccttcgatg ccgcagtata tggatgctat atccatggca gagccgggga
     3181 ctatgccatg gatgaatggg gagaaaccgg gctggtggca ggggatatat tgggtatggt
     3241 accgctggtc tttaaggata tgtacgccct taaaaaggtg cgacccggca gatgtaataa
     3301 agaaccgatt taataacggg gcttaaaggt gtttcccact tctgtaagtg gtgaattccc
     3361 attagctaaa ataggtgggc atatatactc cgcctcgttc acacccgtgc gttgcgattg
     3421 tcagcgcaat tgcttaagga gttataacct atatgggggg gatggcgttt gaaggccttt
     3481 aaatttgctc tgatccttgg atgggtccta tgctttgtag gatgcaaggg gagaacgccg
     3541 gaggatacct ttattgctgc gcagaaggcg ctaatcagcc tagagagcta ttctgctacc
     3601 ctgacatata aggttatgga tgatgaggac gggagggagt atcgctttaa tcaatgggtt
     3661 agcatgcccc attcctttaa aattcaattg attgcaccgg ataacctggt aggaaagacc
     3721 atcataagca acggtaacga gatattgata gagcattcta aggtagagga ctccataaaa
     3781 ttcaatgtca agtccctgga acaacaaaga cccctattca taggcgattt cctcaattcc
     3841 tttttgctgt cggaggaagt cgataaaagg gttcatacag aagatgatat agaatatgtg
     3901 atcctggcct gcccgtcaat gaacaataaa gttcttgggg atacccagga attatggctt
     3961 aaggcgccga agatgatccc tgtcaaattg gttacctacg atgctgacgg cggtatttcc
     4021 agtctaatcc tatttgaaga atttgatagg agctggaagc cggaggagaa tttcttcgat
     4081 ctgtaaggct cttagccttg gaactttagt gattggaata ttattctcca aatttagatt
     4141 atgcatccta tgatttcaaa ttctgttgcc aaactatatg ttttgggata taatattgca
     4201 tatatgctgg gaatgtaacc tatcatatat attaggaggt gctgtcttgg ccgagcttaa
     4261 gaagatattg gttagtttgc ccgacagttt gctacgcgaa gtggatgaaa tcgtggctat
     4321 ggggaagaag aacagaagcg aatttatcag ggaagcaatg aggctttata tcagagaaag
     4381 acagaagatt gagatacgag agaagatgaa aaagggctat gaagagatgg ccaggatcaa
     4441 ttcagaattg acagaagagg gagcaaattc tgatattgag gccttggaaa gatacgaagc
     4501 cgtattgact gggcgtgagt agcgtatgat aaaaagggga gatatttatt atgcagactt
     4561 gagtccggta gtgggatccg aacagggcgg tgtccggccg gtattggtta tacaaaacga
     4621 tataggcaat agatacagcc ctactataat agttgccgct attacatccc agatacataa
     4681 gggtaaactg cccacacata tagaaatcag tgcctctgat tatggtttac ctcgagactc
     4741 tgttatctta cttgagcaga ttagaaccat cgataagaga agactaaagg agaagatcgg
     4801 gtttctggct ccggaaacca tgggcaaagt cgatgaaagc ctccagataa gttttggact
     4861 gatcgatata taatcggatt gcattaatgc aatctttttt ttggaataaa tcattcttag
     4921 acgggagaac aggtcatggt gaaaaggtcc gcatactccg attacatatt tataaccctg
     4981 gggactttag tgacgggttt agcctttaac ctctttttaa tcccccatag gatagccccg
     5041 ggtggcatta gcggtattgc caccatcata tactacataa ccaaaggcag gatacccgtc
     5101 ggcatcagca tgttgggact aaacattccc cttttcatca ttggcattag ggaaatgggc
     5161 cggggattcg gccttaaaac cttaatatcc accattctgt tatcattatt catcgatatt
     5221 ttaaaggttc catccgtaac ggacgagccc atcctggcct caatttacgg cggagtcgtg
     5281 atgggagtag gtctcgggat ggtctttagg ggcaacgcaa ctaccggggg cacagacctt
     5341 tttgcaaaga taatccacaa gcatttccac ttcatcggtg tgggatgggt gctctttgta
     5401 gtcgattcct tggtggtctt aggtgctgcc gtagcctttg gaccgaccct cgccctatat
     5461 gcgttggttt cactgtatct ggggactaag ctcatcgatc ttatactgga gggcatccac
     5521 acggccaagg cctttatatt gatatcggac cgttcggttg agatagccca aaggattatg
     5581 gaggatttag atagaggggt taccctgctc cagggaaagg gagcatatac catggacgaa
     5641 aaggacgtta tcttgtgtgt aacggacagg gtacaggtga ccaagctgaa ggaaataata
     5701 ggtgagatag accctgctgc ctttgtgctg gtagcggatg taagggaagt tatgggggag
     5761 ggattctccc tcgaatagcg ggcataacga ttgccgcaga tgatttaatc agatgtttta
     5821 aaaggaggga taataacaat ggaggaaaga actaacgtgc taaagcccat gaccgtatcg
     5881 gagattctgg acgatacctt tagggtatac ggaaaaaacc tatggacggt tttaatctac
     5941 agcgccgtta taggcggaat tttttccttg atcgtatcct ttataaccgg gcagatgatg
     6001 gtacccgtgg atacattctt taaagattgg atgaatatat tggaaaaccc caatatcgat
     6061 ccggaaactg cctttggacc tattatggaa atgatgccgc gtttttttgc cctccagggc
     6121 atggtaacgc tgttatcgtt aatcggtctt atctttgtaa atccttttat ccaaggcggt
     6181 attataaacg ttacatacag ctatattacg gggcgaagta tgagcatcaa tgaatcattg
     6241 atctctaccc tgaagaaatt ctggaagttg attctgacca gtctttcttt gataccctat
     6301 tttatcgggg tgggcatagc gtttgtcata ctgaccgtca tatccataat tcccctggtc
     6361 ctatctggga tttccatgtc aaaggatccg acggggggca gaattgtggg ctttattttc
     6421 ctgatatttg ttgccgttgc gcttatgata atcttagctg tactggccgg gatgtttata
     6481 acctttacct ttcatgtcgc gaccacggag gagagctatg gttttaatgc catagggagg
     6541 agctttaagt tggtcgccaa aaagttttgg agggttttag ggatcagcct cctaatcaat
     6601 ttgattgtag gcataattgc cgggattata gcccttgttt cgggcataac ctctttggta
     6661 actccatttg ccgctatttc aaattatatg acgggcttta ttaccagcgc ttttataaca
     6721 cccattattc atataagcac cacattattg ttcatggata ccaaagccag ggtagaaccc
     6781 cgggtccatg acggctatta tgagcaaata cagaaccagg gatagttgcg gataaaagga
     6841 acacatgggg gcccaggggc tttgtgttac ccaatagagg tgaaaaaatg gatgtaggta
     6901 tatctacagc tagcttctac ccggacgcat taactgaaca atccatcccc ttgataagag
     6961 ggctgggcgc agaaaaaata gaggtgtttt tggggagttt cagtgaatat gaggaggatt
     7021 tctgtaaccg attacgggat attatagacg agaacggatt atccgtctac tctgtccatg
     7081 ttctgggtac acagtttgag ccccaattct tttcattcac tgaaaggcag agaaacgatt
     7141 cccgggatct gtttttaaag gtcttcaaat gcgccaagat cctaggggcc caggcctatg
     7201 tgtttcacgg cccgcccgtg agggtgaggg caaagcccga tatcgatttt aagaggatcg
     7261 gacctataag cgacgagctg gcatatctgg caggtgatca tgggctaaag ttttcctggg
     7321 agaacgtgtc ctggtgctgg tactcctacc cggagtttgc agacgatctg ctggagcaca
     7381 ccaagggtga taatatcttt tttacccttg atatcaaaca ggccatgcag acgggccatg
     7441 accccatgga tttcttggag cgtatggggg ataggcttat caatattcat gccattgatt
     7501 ttaatgaaaa gcgggaattg accctgcccg ggaggggaat attcgatttt cgaagcctag
     7561 cggataaatt ggagtctata aattatggag gacccatatt cttggaagtt tacaggggaa
     7621 attacgaaga ttatagcgac ctcaaggaat ctatggactt tctcaggggg atattttgtt
     7681 gattaaccaa tgatgccagc tcgttacctt attactaaca aaggtgatga gctattgaaa
     7741 agcggatggc ttcattgatt gggctcctgg aaattggatg ttggagatta gaggctagaa
     7801 gttgggttcc gcggaattgt tactagggag gatggatttg tggaagcagc ttcccgatgt
     7861 agtttggaag atgccggaat aaaccatccg cccatgcaaa acaagcagga cgccttaagc
     7921 cgattccacc ggcaggaagc cgtattggaa gcgataggtt tttcaaatgg aataggcctc
     7981 agtgtggcct atatttttcc accaacgaat tgatccataa ttggtttggt ccctccacta
     8041 tggacatggg ctgatccagt tcttggtttt ccacggggaa aagacgaatt ttaggccctg
     8101 cacggtcctg ggattcccaa acgtttatat gcaacaatac atattcaaca tatccttcct
     8161 tgtaggctat cagggtggct tctccccagg ttttcgggtt aatatccctg taatgagcgt
     8221 cggggactac ttcaatctta atatttgggg tgtacccctt ctcgtcggtt ctgtagctgg
     8281 cgtttgtttc gggaatcact accacagcgc cttcgatggg ttcatcacta aacccgtcca
     8341 gcacctgggg tcttatgtat ccatatttgt ccgctttgct tgtctctacg gactcaacat
     8401 ccccaaggcc ttgtcccata tggataaagg ccgcaaactg tttgcccata tatggatttt
     8461 tttgaacaga gcttccatac catatagcgg ccgccatgat agtgagaatg agcagcccgt
     8521 taaccggcct ttttttcgca tccaaaatta taccccattt ctgtcggaaa tagcattcaa
     8581 tagccattac tgatatatat ctggtcggca caattataat gccggtaaat ttaattgatc
     8641 tattgtttat ccacagtaac ccctggtatt taaatgttta ttcctatacg aacgtattag
     8701 aaccccttta aggtccggaa attaggcata aaaagcatgg gacaattgag aatgcaaggc
     8761 tgcctgacac ttcgcttaat caggacaact ttccaaggac gcgttttcgg tattgtgtag
     8821 gcggcatccc cttatatttt ttaaacatct tggaaaaaag taaggggtcg tcatagccta
     8881 cggaatgagc cacatgggta atggacaaaa gctcatcggt cagcagaata caggccttat
     8941 ccatccgata ttttataaga aagttttggg gagaggtgag acgtatgtcc ttaaacaggc
     9001 tgctgaggta ttttctgtct atacctatat agtcggctat gccctctatg gtgatcttat
     9061 gggagtaatt cttttcaata taatctatag ctttgtccac atataccttt gagtagttac
     9121 gactttcctc cgtatgatgg agggttctct cgttctgtaa gaggagggca aagaaatggt
     9181 atagaagacc ggtcaggacc agctcccgcc ccgtcgttat attctttgtg tcaatcattt
     9241 cttcaacaat cattttaaag gacggattgg gacggcaatc gatgatggga ttatggttgc
     9301 tcaggtttaa cagattcaaa tggtgccggg ccttacgccc ttgaaaaccc acccaacaat
     9361 attcccacgg ctctgtgctg tgggcctggt agtatgaaag ggtgtttggg cagcttagaa
     9421 acccctggcc cgtctttagg tgataggttt tattattata gacaaaaaca cctttgcccg
     9481 aaataatata gtggatgaga taatggtccc tgacccctgt ccaaaaatgg tctcccctac
     9541 atttttcatg gccatagtga tagacgataa gatcatgatc cctatccatg agccggcctc
     9601 tatggcctac gtacgtgtca cagttgtccc tatgcacagc gtcacccccg tttatctgac
     9661 attataccat atatcttgga ctttatacca ttcactttag gggtatagat tataaaatga
     9721 agttaggaag aaaggtttat atggttctgc cccaaaagat tatattttta gcagcgattg
     9781 tatttcatag aaagatggtt tatctaagat gtctttttgg cataaccatt gaatcgaatc
     9841 atggacaaaa tttacgaagg agagtggaga tcagtgaaaa agatagcttt tataggtgcc
     9901 ggcagttttg gttttactag gagcctggtc agagacattc tgtcctttcc ggcctttagg
     9961 gacgctacca tagccctcat ggacatcagc gaagagcgct tatcctacat ccaccaggct
    10021 gtggacagga taatcaagga aggggaatac ccggcaaagg ttatggctac caccgacaga
    10081 atagaggctc ttaaggatgc cgacggcgta attatcacca tactccaggg cggggtccat
    10141 gtatggcgcc atgatgtgga gataccgaag aaatacggtg tcgatatcaa cgtgggagac
    10201 accaggggac cggcgggtat tttcagggcc ctcaggacca ttccagtcat gctggatatc
    10261 tgcaaggaca tcaacgaata ctgtcccaag gccatcgtac ttaactatac caaccccatg
    10321 gccatgctgt gcagggctat gcaggccgaa tatccgaacc tggctatatc cgggctttgc
    10381 cacagcgtac agggaacggc acatatgctg gcaaaatgga taggcgcacc caaggacgag
    10441 attacctaca aatgtgccgg cataaaccat caggcctttt atttggattt taaatggaac
    10501 aacgaggatg cctatcccct gattcacaag gccatcaccg agaaccagga tatatacaac
    10561 gaggaacagg tccgtaacga aatgtatctg cacctagact attatgtaac cgaatccagc
    10621 gggcacaact ctgaatataa tgcctggttc agaaagagac ccgatctcat tgagaagtat
    10681 tgcacccatg gtaccggttg gaaccccggt gtatacgcgt acatcttaga cgaatatttg
    10741 gccagggagg ataactggaa ggacgatata gaagaatggc tgaaccagga cgaaatcgac
    10801 ctggaaaggg gcgaggaata cgcagcctat atatttaacg ccatatttgg tgacggaacc
    10861 atgttcgaat ttaacggcaa tgtccgtaac tttggtctta tcgagaacct gcccgaaggc
    10921 tgctgtgtag aggtgccggt acttgcctct aagcgaggtc tggatcccat acacgtgggg
    10981 ccgctaccgg aacatctggc catcttaaac aatatcaatg ccggctgtga agagcttgcc
    11041 gtggaagggg ccctagaagg ggataagaga aagatattcc acgcagtttg cttggatcct
    11101 ttgacatcgg cggtattgag cctgcaggag atccaggata tggtggacga gatgtttgaa
    11161 gccaacaagg attggttgcc ccattttgac atttaagacg gtatagcggg gtttgtcgcc
    11221 aagcctctaa agggacggcg atggaattaa ttttgattag agttttttac aaaatat
//
LOCUS       DASRNX010000176        18682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_672600,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000176 DASRNX010000000
VERSION     DASRNX010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 18682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18682
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672600"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(477..2849)
                     /locus_tag="VFD89_07025"
     CDS             complement(477..2849)
                     /locus_tag="VFD89_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HZJ57977.1"
                     /translation="MDQRTLRVLEYHKIIGLLEDFVLSELGKSAVGKLNPSSSFARIQ
                     KMLDETSQAHAIINHCGYSPMDIFPDISTILQKAKIGSVLSAGELLKVGLVLRISGRV
                     KGGIEEYPYPEAVPHILHKSKALKLHKIQMDEIFRCIETEDRISDYASDRLANIRRRI
                     TRANERIRERLNSLIRSPQFQKLLQEPIVTIRNERYVVPVKQEHRGSVPGMIHDQSAS
                     GATVFIEPMAVVEANNELRELMIEEQREIEKVLLELTMEVQSSYETILNNLELLAALD
                     VIFAKASFSRSYKGIHPEVVPEPYIDIINGRHPLIDKDEVVPITVSLGERSTCLIITG
                     PNTGGKTVTLKTVGLFALMTQSGLHIPADYGSKMGIFEGVFADIGDEQSIEQSLSTFS
                     SHMVHIANIMEEADDKSLVLFDELGAGTDPIEGAALAMAILNFLCARRTCTIATTHYS
                     ELKVFAMTQAGMENASMEFDIDTLRPTFRLLMGIPGKSNAFEIARRLGLKEKLIDAAR
                     EFISQDEKHFEDVLSDMEKSRIQAKEDQEEAKAHLDEIRAIRGKLDEKERLIKGDEEK
                     ILRDARGEARNILRKAKDQADEILHEMRMLANQSQDKEQRREMEQARKRLKRSLDSME
                     EAIGDSLKPKDGLLIPPKNLKPGESVYIANLDQTGQVLSISAQGDEVQVQVGIMKINV
                     HISNLKRVQNESRDSILRTSSVAIKNKAISMELDLRGQDLEGAIVNVDKYLDDMFLAG
                     LQEVVLIHGKGTGVLRNGIHKHLRQHPHVDSFRLGRFGEGESGVTIVELK"
     gene            complement(2987..3214)
                     /locus_tag="VFD89_07030"
     CDS             complement(2987..3214)
                     /locus_tag="VFD89_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57978.1"
                     /translation="MEDRQMDQVITSIENNIKKCDILNRVLKTKIAGEDIDFNWYYKR
                     GYSEKQVLVGTELVDLYLKLKSHLNSPDDSR"
     gene            complement(3735..4073)
                     /gene="zapA"
                     /locus_tag="VFD89_07035"
     CDS             complement(3735..4073)
                     /gene="zapA"
                     /locus_tag="VFD89_07035"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HZJ57979.1"
                     /translation="MGRKNKVIVRIGGREYAMRGSESEEYIHKVAIYVDKKMEEISAK
                     QPPLSISMLAILTAINLADEVIKQKDEIQSLKRELNEVKSLLKMTEGDRKIAEGDRTN
                     IYDMPKKMRR"
     gene            complement(4314..6707)
                     /gene="pheT"
                     /locus_tag="VFD89_07040"
     CDS             complement(4314..6707)
                     /gene="pheT"
                     /locus_tag="VFD89_07040"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZJ57980.1"
                     /translation="MLVPIKWLKDYIDLDVSAQELADAMTLTGSNAEGVMRLAVDIEK
                     VVVGKIIKVKQHPNADKLLICEVDVGDEVLQIVTGAPNVAPNSYVPVAVDGALLPGGI
                     RITAGELRGEISQGMMCSASELGLAQLGYIDNGVDGILILHEEYPLGMKINDALELDD
                     EVIDFEITSNRPDCLSMVGIAREAAVTLETSYREPEIVLDPGIGNIDDEAKVIVEDPH
                     LCSRYCARVVKDVKIGPSPRWMRRRLAAAGIRPINNIVDITNFVMLELGQPMHAFDID
                     KVAQRTIVVRKAEQNERLITLDEQARDLTEDMLVIADPEKAVGIAGVMGGYNTEITPG
                     TRNIILESAKFDGGSVRLTSKALGLRSEASSRFEKGVDIINVERAIDRAVQLIQNLDA
                     GTVVEGKIDVCSDDLAPRIIKTQWKKINRLLGLKLDRDEICGILEALSFKAEIVGDSL
                     KVIIPSFRQDVEGVADLAEEVARIYGYDRIPMTLMENSVALGSRTREQILTNKAKNVL
                     AGMGLYESITYSFVSPKVYERIGFEPREHPETVIIENPLGEDQSSMRTTLIPSILEVL
                     SRNQNRSIEVCKVFEISNIFIPKSQPMTELPIERLTLGMGQYGEDVDFYSLKGQLEAL
                     FSVFGFGDEIEFVPQLHPTFHPGRTAGIMLSGRKLGILGQVHPSVCQTYSLDDNVMLS
                     ELDFALMLEQAKTEKQFTRLPRYPAVTRDLAIIVNKKVLASSIEKLIVDSGGKILEGV
                     ELFDIYEGDQIPEGHRNLAYSLSYRAWDRTLKDTDINHIHDRIISALETKFEAQLRK"
     gene            complement(6794..7813)
                     /gene="pheS"
                     /locus_tag="VFD89_07045"
     CDS             complement(6794..7813)
                     /gene="pheS"
                     /locus_tag="VFD89_07045"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZJ57981.1"
                     /translation="MREKLDDIREQALGSIEDARQSVNLEAIRIKYLGKKGELTRILR
                     GMGKLSPEERPVMGQLANKVRGDIESALSLKIREMEQIALTNRLKEEAIDVTLPGKKT
                     PLGRLHPLTTVLNQITDVFLGMGFNVAEGPEVELDYYNFEALNMPKNHPARDTQDTFY
                     INENILLRTHTSPTQIRVMEEQAPPIKIIVPGRVYRSDAVDATHSPIFFQIEGLVVDK
                     AITMGDLKGVLDIFAKKMFGEQTRTRFRPHHFPFTEPSAEMDVSCVTCGGSGCRICSN
                     TGWIEILGAGMVHSRVLEYGKIDPNEYSGFAFGMGLDRIVNQIYGIDDIRLLYENDIR
                     FLRQF"
     misc_binding    complement(7887..8107)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8104..8220)
                     /locus_tag="VFD89_07050"
     CDS             complement(8104..8220)
                     /locus_tag="VFD89_07050"
                     /inference="COORDINATES: protein motif:HMM:NF025372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HZJ57982.1"
                     /translation="MRIQRRWFPVLADAIWRIFEATGSVSAYMLYKKLITLR"
     gene            complement(8245..9069)
                     /locus_tag="VFD89_07055"
     CDS             complement(8245..9069)
                     /locus_tag="VFD89_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZJ57983.1"
                     /translation="MSKIITSPNNPTIKLTRSLHLKKYRDEHGLYFVEGPKMVREALN
                     RKDTIHTLIYSEDYNTQGLGLDVRDSNVPITCVDEKIFRQISDVKSPQGIIGILRKKK
                     YDMGMVFGQSPGFWVILDRIQDPGNMGTIIRTIDAAGGSGVILLKGCVDPYNPKTIRS
                     TMGSIFRVPIIEIHDNEDFFFKLIENNVDILASAMEGDSIFNWEGRRDRGIKALIIGN
                     ESRGISEEIRGLATSIISIPIIGDAESLNASVAAGIMIYEIMRKEGNLGRKFSCIC"
     gene            complement(9174..9533)
                     /gene="rplT"
                     /locus_tag="VFD89_07060"
     CDS             complement(9174..9533)
                     /gene="rplT"
                     /locus_tag="VFD89_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZJ57984.1"
                     /translation="MARVKGAVNARKKHKKVLKLAKGYFGAKSKQFRTANQAVMKSLS
                     YSYTGRKLRKRDFRKLWITRINAAARINGLSYSRFINGLKLANVQIDRKVLADMAVND
                     SNSFTQLVTLAKQKLDA"
     gene            complement(9555..9752)
                     /gene="rpmI"
                     /locus_tag="VFD89_07065"
     CDS             complement(9555..9752)
                     /gene="rpmI"
                     /locus_tag="VFD89_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZJ57985.1"
                     /translation="MPKIKTHRGAAKRFSLTKSGRVKRAKAYKSHILTKKTTKRKRNL
                     RKGAYIAAAEEKNIRRLIPYK"
     gene            complement(9775..10293)
                     /gene="infC"
                     /locus_tag="VFD89_07070"
     CDS             complement(9775..10293)
                     /gene="infC"
                     /locus_tag="VFD89_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZJ57986.1"
                     /translation="MNINNRELSVNEGIRDREVRVIDANGEQLGIMSPRKAMEVAEQR
                     QLDLVKVAPQAKPPVCRIMDYGKYRFEMAKKEKEARKNQRTISVKEVRLSASIEEHDM
                     GVKAKNADKFLKAGDKVKVTIRFRGREMAHTSIGYDVMNKFSKMVTQENAVERRPRLE
                     GRSMIMILAPKE"
     misc_feature    complement(10506..10633)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(10829..12733)
                     /gene="thrS"
                     /locus_tag="VFD89_07075"
     CDS             complement(10829..12733)
                     /gene="thrS"
                     /locus_tag="VFD89_07075"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZJ57987.1"
                     /translation="MIKITLKDGSSKEYRNGITVGEVATDISKGLARAAVAGQVDGQT
                     VDLMHPLHEDCELNILTFEDIEGKKAFWHTTSHVMAQAVKRLFPNIKLAIGPSIDNGF
                     YYDFDTQRPFTPEDLEFIESEMTKIIKENLELERFELPREEAIDFVAEKDESYKIELI
                     EDLPEDSVISFYRQGEFVDLCAGPHVPSTGKIKSFKLLNLAGAYWRGEESNKMLQRIY
                     GVSFPKKSQLDEYINRLEEAKRRDHRKLGRELDLFSIHEEGPGFPFFHPKGMVLRNTL
                     EEFWRKEHVKWGYEEIRTPIILSRDLWIRSGHWDHYQENMYFTNIDEADYAVKPMNCP
                     GSMILYKRKMFSYRDLPLRMGELGLVHRHELSGTLHGLMRVRCFTQDDAHIFMLPEQV
                     KDEIVGVIDLVDYFYKQFGFNYHVELSTKPEDAMGSDEDWDVAIDALKEALEERNMKY
                     VINEGDGAFYGPKIDFHLEDSIGRTWQCGTIQLDFQMPERFDLTYVGADGEKHRPAMI
                     HRVVFGSIERFIGILIEHYAGAFPVWLAPVQVRLLSITDRSAEYIKKLVTDLEESGVR
                     VETDLRNEKIGYKIREARLQRIPYMLVVGDKEVESRQVAVRSRDEGDLGPMGVQDFMD
                     RIRQEIQQRL"
     gene            complement(13239..14096)
                     /gene="aroE"
                     /locus_tag="VFD89_07080"
     CDS             complement(13239..14096)
                     /gene="aroE"
                     /locus_tag="VFD89_07080"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZJ57988.1"
                     /translation="MIISPKTSYAAVIGHPIAHSLSPKLHNSIYDKLGLDMVYLAFDI
                     SHRDLQSAVGGLRSLNFSGFNVTIPYKIEVMDYLNEIDPMAIAIGAVNTVKIEDGRMI
                     GYNTDGLGFLSSLKNRNIRCHGRRILIIGAGGSARAIAITLAEDSPKEILILNRNAAR
                     GEQLAEDINRYAGRDLSTVVEEAPATIDIIINTTPLGMWPNVQNSPLSGYSFSPETVV
                     CDIVYNPLETTLLKEARKSGSITVGGIGMLVGQGIKAVEIWTGRGIDDSISGLVYGEI
                     HNSLKESSQ"
     gene            complement(14093..14587)
                     /locus_tag="VFD89_07085"
     CDS             complement(14093..14587)
                     /locus_tag="VFD89_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HZJ57989.1"
                     /translation="MREALKPNESHDSVFTIDLAGLYERGYKNILIDVDNTITPWNAD
                     RVSERLRGWLKQCQILGFTVCLFSNGTSSRVNRLAAELMISAVPKGGKPLRAAFKRAL
                     GYIDGRPQNTIMIGDQIFTDIWGGNLTGLYTILVNPISTREFWGTKFNRFLERVLAKR
                     GELG"
     gene            complement(14935..15648)
                     /gene="sigK"
                     /locus_tag="VFD89_07090"
     CDS             complement(14935..15648)
                     /gene="sigK"
                     /locus_tag="VFD89_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HZJ57990.1"
                     /translation="MLIAAGGIVSFLKCIYFLASHVLGTHSFPQPLGPEEEAHHIRLC
                     AQGSEDSKNTLIEHNLRLVAHIVKKYANTGKEVDDLISIGTIGLIKAISTFNPERKTR
                     LATYAARCIENEILMTIRADKKIRGEISLQDPIGVDKEGNEISLLDVLGSETDSVLDE
                     VELKLQIHRLYDKMQSVLKQREKMVLEMRYGLINGKNKTQREIAQILGISRSYVSRIE
                     KKAIMKLGKEFSGDGFGIG"
     gene            complement(15793..17466)
                     /locus_tag="VFD89_07095"
     CDS             complement(15793..17466)
                     /locus_tag="VFD89_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZJ57991.1"
                     /translation="MKKETITHKRVIFFGLALSILFTALVMRLLYIQLIMGGHYKQKA
                     HSQGILPIVVKHARGDILDRNGIVLTRGRLKAYLLIFPAYFNDRNSLYDIVGPMTGLG
                     LDELSDSVLGGSPFIKCEILNPDPKIERSIRQGEYPGLMIDYETQRYDDFTLARHLMG
                     YFKKSDNAPQSGIEKEYDAFLRSDDVLTVYAMTDAKRRILSGTVHRNKSKNDEYYDIG
                     LTIDYYVQSALERAMDSHAQKAGGVVVEVETGDILAIASRPNFDQNDIETAKNHKDSL
                     WAVPVKAFSPGSLFKIIVATAGIENGICNGETMHICQGGITINSVRYSCHSERGGLGD
                     ISMRQAFAHSCNDAFIGIASRVGGDSIIKMAQKMGLGQPLDIGIHNERGSLPQKVEYA
                     GAGIGNLALGQGKVEVTPIQIAQMMSIIASDGIKKDLSLIREITDQSGYRVENIYEEQ
                     TGERLIKSGTARELRAWMGDVTEYGTGIKAYSVKAGGTAGKTGTPQIFGDADAGYYGW
                     FAGFFPTDTPKYAIVILVREESSGGEKAALIFRDTADEITRYTEKLRIP"
     gene            complement(17529..>18682)
                     /locus_tag="VFD89_07100"
     CDS             complement(17529..>18682)
                     /locus_tag="VFD89_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HZJ57992.1"
                     /translation="VYMAGKRFGLRAFAGNFQRDEINEAVEYAHSINKKVYVTMNIFA
                     HNEDFKDMDEYMKFLSKIGVDAAIISDPGILSLAREAMPNVPIHMSTQANNTNWRSAK
                     FWHEQGVKRIILARELSLNEIGQIRENVPDTLEIETFVHGAMCISYSGRCLLSNVFTG
                     RDANRGQCAHPCRWKYSIVEEKRPGEYYPVLEDDRGTYIMNSKDLCMLGHIPQLMGTG
                     IDGFKIEGRMKSSYYVATVVQAYRREMDAYLEDQDGYRWDPKAIAEIQKASHRAYTTG
                     FYFKKPAGEDHQYSDSSYIRNYDFVGMVLGYDKERGLMEVEQRNAFEVGDVLEIMMPG
                     EDYMDYTVTTILDEEGRPINRAPHPQQRVYLPLDRGITGYSILRREQKV"
BASE COUNT         4732 a         4801 c         3395 g         5754 t
ORIGIN      
        1 ccataatggg catcattaat aagagcatta tatacattat aaatataatc agggaaatct
       61 ccctgtttgc ccttaggaaa ttgcctttta taagcatata aacaaatgcc aaccctggga
      121 tgttccccag ggacacgaga gatagccccc tgacgaatct tctcccattg atcaaggaac
      181 caaatgtttt tccgtaatga aaccaaaacc taaagcatgg gattccggtc cccagcagta
      241 taagccaaac aggcaatggg accggtaatc ctaaatgacg gacccaatat atcgccttga
      301 ataaaccaaa ggaaaggcat cctatgatca aataaatgaa aaaattgata accgcaagtc
      361 tcgcaccctc cgctatgtga ctgctatgtc gtggaaaaag cgccttcgtt ctaaatccca
      421 tgagcacccc accttatctg tatgtctaca cattgatatt tccctgtaat ttaccgttat
      481 ttaagttcaa ctatagttac ccccgattca ccttcaccga acctacccag cctaaaggaa
      541 tccacatggg ggtgttgcct caaatgcttg tgtatgccgt ttctcaaaac tcctgtgccc
      601 ttcccatgga ttaaaactac ctcctgaagt ccggctagaa acatatcatc taaatattta
      661 tccacgttta ctatagcccc ttcaaggtcc tgccctctca aatccaattc catggatatg
      721 gctttgtttt ttatagctac tgagcttgta cgcaatatgc tgtctcttga ttcattttgt
      781 actctcttaa ggttggaaat atgcacattt atcttcataa ttcctacctg tacttgtacc
      841 tcatccccct gagcggatat actaaggact tgccctgttt ggtccaagtt agcgatgtag
      901 actgattccc ccggtttcaa gtttttcggc ggaatcaaga ggccatcctt gggttttagg
      961 ctgtcaccaa tagcctcttc cattgaatcc agacttcgct ttaacctctt tcgtgcctgc
     1021 tccatttccc tgcgctgctc tttatcctgg ctttgatttg ccagcatacg catctcatgc
     1081 aggatctcat cagcttgatc cttggccttt cgcaaaatat tcctggcctc accacgggca
     1141 tctctgagaa ttttttcttc atccccttta attagcctct ctttttcatc taatttaccc
     1201 cttatcgctc tgatttcatc caggtgggct ttagcctctt cctgatcttc ctttgcttgg
     1261 atcctgcttt tctccatatc agataacaca tcttcgaaat gtttttcatc ttggcttata
     1321 aattcccttg ctgcgtctat caatttctct ttcagaccga ggcgtcttgc aatctcaaat
     1381 gcattgctct tgcccgggat gcccatcaag agccgaaatg tggggcgcag ggtgtctata
     1441 tcaaattcca tggatgcatt ttccataccc gcctgagtca ttgcaaaaac ctttaattca
     1501 ctgtagtgag tggttgcgat agtacaagtc ctccttgcac agagaaaatt aagaatagcc
     1561 atggccaaag cagccccttc aataggatca gttcctgccc ccaattcatc aaagagtaca
     1621 agggatttat catcagcctc ttccattatg ttagcgatgt gaaccatatg ggaagagaaa
     1681 gtgctgagac tttgttcaat gctctgttca tccccaatat ccgcaaaaac cccctcaaat
     1741 atacccatct tactgccgta atcggcaggt atgtgtaaac ccgattgggt cattaatgcg
     1801 aacaaaccga ctgttttgag ggttaccgtc tttccaccgg tgttaggacc ggtaataatc
     1861 agacatgttg atctttcacc caaacttacc gttatcggca ctacctcatc tttatcaatc
     1921 aagggatgcc gcccgttgat tatgtctatg taaggctccg gtaccacctc cggatgaatc
     1981 cccttgtagg aacggctaaa gctagcttta gcaaatatca catcgagcgc ggctagtagt
     2041 tccaggttgt ttaatatggt ctcgtaggat gactgcacct ccatagttaa ttcaagcaga
     2101 accttctcta tctccctttg ctcttcaatc atcaattccc taagctcatt gttagcctca
     2161 acgacagcca taggctcaat aaacaccgtc gcaccactgg ctgattgatc atggatcatg
     2221 ccgggaacac tgcccctatg ttcctgcttc acaggtacaa catagcgttc gttcctgata
     2281 gtcactatgg gttcttgcaa aagtttttga aactggggtg agcgtattaa actgtttaac
     2341 cgttcccgaa tcctttcatt ggctcttgta atacgtcttc ttatatttgc tagccggtcg
     2401 ctcgcatagt cggaaatccg atcctcagtt tctatgcacc gaaaaatctc gtccatttga
     2461 atcttgtgta actttaatgc ctttgactta tgcaatatat ggggaacggc ctccggatac
     2521 ggatattctt caattccgcc cttcacccgc ccggatatcc ttaatacaag ccctaccttt
     2581 agaagttcac cggcggacaa tacagatcca attttggcct tttggagaat agtactgatg
     2641 tcggggaaaa tgtccatggg ggaatatccg caatgattaa ttatagcatg ggcttggctt
     2701 gtctcatcca gcatcttctg gatcctagca aaactggacg aaggattaag cttccccacc
     2761 gcactctttc ctaattccga taaaacaaag tcctccaata acccgataat tttatgatac
     2821 tccaataccc tgagagtcct ctgatccaag ttcattacct cctaaattct aacccctaat
     2881 cccatagttc atctttcttt gacaagataa cagcacaaat tctaaaatct ccacataaaa
     2941 aaaggcgcac aaatgcacca tgaacgaatt attgcaaaat aaaagactat ctggagtcat
     3001 cgggactatt caagtggctt ttcaatttga ggtacagatc cactaattca gtccctacca
     3061 aaacctgttt ctcgctataa cctctcttat aataccaatt gaaatcgata tcttctcctg
     3121 caatttttgt ttttaacacc cggtttagaa tatcgcactt ttttatattg ttttcaatac
     3181 ttgtaatcac ctgatccatt tgcctatctt ccacgagaaa tcccccatct atcaatttat
     3241 ccctaccatg aaatgtggtc gatgatgcaa tctgcaaaat aaatctagca aaaggctttc
     3301 gtaattatcg caacgaacat catatttcta agattcagca tttgcgatag aaatccttta
     3361 tttccccaat atttcccttt aagattccat ctttagataa acaataggtc aaataaattc
     3421 gcctatggat gataaatttt atagtcgaag tcgtttatct ataaatcgcc cacatagttg
     3481 tagtatattc atatttttcc caaccataca ttaatctatc caaaatacaa caatttacta
     3541 tgacctatgg agtttgggaa aaaggttgta aagtgaatta gggtatgatt taaattaagc
     3601 ttttcaagat cccaagctga ataaccattt ttgatagcat ccaatggggt gttttattct
     3661 tgtatagggt attccccccg gaaaacagat atgtgcaagg aacacagtta ctataatgac
     3721 cgggtacaaa acctttagcg ccgcattttc tttggcatat cataaatatt cgttctatcg
     3781 ccttcagcaa tttttctgtc gccttcagtc atttttagca aactcttaac ctcattcaat
     3841 tcccttttaa ggctttgtat ttcatccttt tgttttatta cctcgtcagc taaattaata
     3901 gccgttaaga ttgcgagcat agagatacta aggggtggtt gcttcgcaga gatttcctcc
     3961 atttttttgt ccacataaat ggctacctta tggatgtatt cctcggattc gcttcccctc
     4021 atagcatatt ctcttccgcc gatcctaacg ataaccttgt tttttctgcc caataccgac
     4081 gtcccccttc taaaatattc ttgatatatt ttgtcaaaac aagaagcagg atgttggata
     4141 acttgggtat attatatatt acttgacatt cttatacaag cgtattgtcg aaataaatac
     4201 aatctttgta tggaccccac cgttatacat accaatggaa taacggtctt tacattatgg
     4261 ccccggagat tataagggca gacgttgacg tctgccctta tcgtttgttt cctctatttc
     4321 cttaactgag cttcgaattt agtttcaagg gcacttataa tcctgtcatg aatatgatta
     4381 atatccgtat cctttaatgt tctatcccat gccctatagc ttagggaata ggctaaattc
     4441 ctatgaccct cggggatctg gtctccctca tagatatcaa agagctctac tccttctaaa
     4501 atcttacccc cagaatccac tattagtttt tcaatgctgg atgccaacac ctttttgttt
     4561 actattatag ccagatccct agtaaccgca ggatatctgg gaagtctggt gaactgcttt
     4621 tctgttttcg cctgctccag cattaatgcg aaatccagtt ctgacaacat cacattatca
     4681 tcgaggctat aggtctgaca tacgcttggg tgtacttgtc ccaatattcc caatttccgg
     4741 cccgaaagca tgatcccggc cgtcctaccc ggatgaaaag tcgggtgtaa ctgcggtaca
     4801 aattcaattt cgtccccaaa tccaaatacg gaaaacaggg cttccagctg accctttaga
     4861 ctataaaaat caacatcctc gccatattgt cccatgccta aggtaagtct ttctataggc
     4921 agctccgtca tgggctggga tttgggtatg aatatattgc taatctcaaa caccttgcat
     4981 acctcaatac tgcgattttg atttctggac aatacctcca gtatgctggg gatcaacgta
     5041 gtacgcatag agctttgatc ctcccccagg gggttctcaa ttattacagt ttcgggatgt
     5101 tcccgaggtt cgaatccaat cctttcatat acctttggac tcacaaagga atacgtgatg
     5161 gattcataca gccccatacc agcaagcaca tttttggcct tattcgtcaa tatttgctcc
     5221 cttgtcctcg aacccaaagc aacagagttt tccatcaagg tcatgggtat cctatcgtat
     5281 ccgtagatcc tcgctacctc ctccgccaga tcggcaacgc cctctacatc ctgcctaaag
     5341 gatgggatta ttaccttcag ggaatcccct acgatttctg ctttaaagga taatgcttct
     5401 aagattccac atatttcatc gcgatccagt ttcagcccca gtaatcggtt gatcttcttc
     5461 cattgggttt tgataatacg gggtgctaaa tcatcggaac aaacatcaat cttcccctct
     5521 accacggtac ctgcgtccag attttgaatc aattgtaccg ccctgtcaat tgccctctcc
     5581 acattgatta tgtcaacacc tttttcaaaa cggctagagg cttcactcct aagcccaagt
     5641 gctttggaag tcaaccttac gctcccgcca tcgaacttgg cgctttccaa tatgatatta
     5701 cgggttcccg gggttatttc ggtgttatat ccccccatca ctccggcaat gcctacagcc
     5761 ttttccggat ctgcgataac tagcatatcc tctgtgagat ccctggcttg ttcgtccaag
     5821 gttatcagcc tttcgttttg ctcggccttc cttaccacta tagttctttg ggctacctta
     5881 tcgatatcaa aggcatgcat gggctgcccc agctccaaca taacgaaatt ggttatatct
     5941 acaatgttat tgataggcct tatacccgcg gccgccaacc ttctcctcat ccagcgcggc
     6001 gatggtccaa ttttcacgtc cttgaccacc ctagcacagt atcggctgca aaggtgagga
     6061 tcctccacga taaccttggc ctcatcgtct atattcccta tacccggatc caagacaatc
     6121 tccggctccc tataagaagt ctccagggta acggcagctt cccgtgctat gcctaccatg
     6181 cttaaacagt ccggtcggtt ggaagtgatc tcaaaatcaa ttacctcatc atccaattcc
     6241 aaggcatcat ttattttcat tcccagggga tattcctcat ggagaattag aatcccgtcc
     6301 actccgttat caatataccc cagctgagca agccccaatt cgcttgcaga gcacatcatg
     6361 ccctgagaaa tttctcccct cagctcacca gccgtgattc gtattccgcc gggaagcaat
     6421 gcaccgtcaa ccgctacggg tacatagcta tttggcgcta cgttgggggc gcccgtaaca
     6481 atttggagca cctcatctcc gacatccact tcacatatca ataacttgtc tgcattggga
     6541 tgctgtttta ccttgataat tttccccact acgaccttct caatatcaac tgctaatcgc
     6601 attacaccct cggcgttgga accggttaag gtcatggcat cagccagttc ctgtgcggat
     6661 acatccaagt ctatataatc ttttaaccat tttattggta ctaacatcgc tcttctcctc
     6721 tccaactcaa tctatatgcc acgttaagga tttatccggg aagctctaaa aggcccgatt
     6781 gtctttaggc ccattaaaac tggcgtaaaa atcttatatc attttcatat aacagccgta
     6841 tatcgtctat gccgtatatc tgattgacga tcctatccag tcccatccca aaagcgaatc
     6901 ccgaatattc atttggatct attttcccgt actccaacac tctagagtgc accatgcctg
     6961 ctccaaggat ctcaatccaa cccgtattgg agcatatcct acatccgctg cctccgcatg
     7021 ttacacagct aacgtccatt tcagcgctgg gttcagtgaa gggaaagtga tggggcctga
     7081 aacgggtcct agtctgttcc ccaaacattt tctttgcaaa gatatccaat acgcccttta
     7141 agtcccccat tgtgatagct ttgtctacta ccaatccctc tatttgaaag aaaataggcg
     7201 aatgggtggc atcaacggca tcggatctgt atacccttcc cggtactatt atctttatcg
     7261 gaggagcctg ttcctccata acccgaattt gggtaggtga ggtatgggtg cgcaacaaaa
     7321 tattctcatt tatataaaag gtatcctggg tgtccctggc aggatgattt ttgggcatat
     7381 tcaatgcttc gaaattgtag taatccagct ctacctccgg cccctcggct acattaaatc
     7441 ccatccctaa aaacacatca gttatttgat ttagtacggt tgtcaacggg tgcagcctac
     7501 ccaggggcgt tttctttccg ggcaaggtga catcgatagc ttcctccttt aatctattcg
     7561 tcaaggctat ttgctccatt tcccttatct taagggataa ggccgattcg atatcccccc
     7621 taactttatt agctaattgg cccataacag gccgttcctc cggagatagc ttccccatac
     7681 ccctcagaat ccgggttaat tcgccttttt tgcccagata cttaatcctt atcgcttcca
     7741 ggttgacgga ttgacgggca tcttctatac tgcccagcgc ttgttcacgt atatcgtcta
     7801 acttctctcg cacaatctgt tactcctttc tatgctatga ttcccctgta acggagccat
     7861 aatataaata aaacccccat ccctaaatat gggacggagg taactttatc cgcggtacca
     7921 cccaagttca tttgtacaaa acaaacctct tgaattcata acggctatgc cggttagacc
     7981 tactaccact tcagcctaca gctcaagagc gaacttccat tttctgatta tagggatgct
     8041 tccagcccat gacacccctt ctctggataa taagggaaaa tgtacttctc tccgtcattg
     8101 cttttatcta agggttatta attttttata aagcatatag gcagaaacgg aacccgtagc
     8161 ttcaaatatc ctccaaatag cgtcagccag cactgggaac catctccttt gaatccgcat
     8221 ttttaattat tatagcatat accttcagca gatacaagaa aatttccgcc ctagattacc
     8281 ttcttttctc attatttcat atatcataat gcctgccgct accgaggcat tgagggattc
     8341 cgcatcgcct ataataggta tcgatatgat actggtggcg agcccacgaa tttcctcgct
     8401 aatgccccgg gattcgttac ctattatcag agcctttatg cccctatccc tgcgcccttc
     8461 ccagttgaat atgctatcgc cttccatggc gcttgccaga atatctacgt tattctctat
     8521 cagtttaaaa aagaaatcct cgttgtcatg gatttcgatt atgggaaccc gaaatataga
     8581 acccatggta gacctaatag tcttgggatt gtacggatca acacatccct taagtaatat
     8641 aacaccgctt ccgcccgcgg cgtctatagt tctaataatt gttcccatat ttcccgggtc
     8701 ttgaattctg tccaggatga cccaaaatcc tgggctttgt ccgaacacca tacccatatc
     8761 gtactttttc ttcctgagta tgccgattat cccttgggga ctcttgacat cgcttatttg
     8821 tctaaatatt ttttcatcga cacatgttat cgggacgttt gaatccctca catcaagccc
     8881 cagcccctgt gtattgtaat cctctgaata gattagggta tgaattgtat ccttccgatt
     8941 cagggcctct ctcaccatct ttgggccttc tacaaaatat agtccgtgtt catccctata
     9001 ttttttcagg tgtaacgatc ttgtaagctt tatagttggg ttatttgggc tggtaataat
     9061 tttgctcatg gtatgttcgt ctccaccttt cataggctaa attgcaggtt tcaacttgaa
     9121 tgatgtcttt tgctttatgc ataaaaaaag accccaatgc agaggtcttt gatttatgca
     9181 tctagtttct gttttgccaa agtaaccaat tgagtaaaac tgtttgaatc atttaccgcc
     9241 atgtccgcta ataccttgcg atcgatctgt acattcgcaa gttttaatcc attaataaat
     9301 ctgctgtagc tgagtccatt tatacgcgca gcggcgttga ttctggttat ccatagcttc
     9361 ctaaaatccc tttttctcag ttttcttccc gtatatgaat aggacaagga cttcatcact
     9421 gcctgattag cagtcctaaa ttgtttgctt ttcgcgccga agtaaccctt ggccaatttt
     9481 agtacctttt tatgtttctt tctggcattt actgcacctt ttactcttgc catgtctcta
     9541 cctccttaac tgccttattt ataaggaatc aatctcctga tgtttttttc ttcagcggca
     9601 gcaatatagg cgccctttct gagattcctc ttcctctttg tagttttttt ggtcaatata
     9661 tgactcttat atgcttttgc tctctttact cttccagatt tagtcaacga aaaccttttg
     9721 gctgcccctc tgtgtgtctt gatttttggc atagggcttc ctcctctctt gcaattattc
     9781 cttgggtgct aagatcatga tcatgctcct gccctcaagt ctgggtctgc gttcaacagc
     9841 attttcttgg gtaaccatct tggaaaactt gttcattaca tcatacccga ttgatgtatg
     9901 ggccatctca cggcctcgga aacggatagt tacctttacc ttatcgccgg cctttaaaaa
     9961 tttatcagca ttctttgctt ttacgcccat atcatgttct tcaatgctgg cagacagcct
    10021 tacctcttta acgcttatgg ttcgctgatt tttgcgggct tccttttcct ttttagccat
    10081 ctcaaagcga tatttaccat aatccatgat tctgcataca ggcggctttg cctgaggcgc
    10141 aaccttcacc aaatccaact gtctttgctc ggcaacctcc attgcttttc ttggagacat
    10201 tatacccagc tgctccccgt tggcatctat gacccgaact tccctatctc ggattccctc
    10261 gtttactgag agttccctgt tattaatatt cattcacctc cgctattttt taacaagtaa
    10321 ttttaacatt atttcatttt gatcacgtac tgaaaaaaca ataattgggt taaagtccca
    10381 aacagttaac caccgataaa acggggtaac ccggtttatg ttttcttatt gaaacttccc
    10441 caccgttatg aaccaatccc atacaagtgt atgcatgagt ttggtgacaa tggcctaaaa
    10501 tatccacaca acaaaaagag tgggcgactt ttacccactc taaataactc attcaaatcc
    10561 aatacagatt taatagtgtt aacctccctg gcagtgccga aaggtgagaa gtggatatct
    10621 tctactttag ccagatatag actatcatat agcggctctc atgtcaaata aatattttcc
    10681 taagggtaca tgatccaaat ttaaagaata cataggctac gcccttttcc tataagccgc
    10741 gccgctaaca caagcccagg gtaaatgcct tttccccaca tttaatatac tgcccattac
    10801 cggtatatca atgaaccatg gcgttaatct atagcctttg ttggatttcc tgccgaatcc
    10861 tgtccataaa atcctgtaca cccataggtc ctaaatcccc ctcgtccctt gacctgaccg
    10921 caacctgtct gctttcgacc tccttgtctc ccaccaccag catgtagggt atcctttgga
    10981 gtctggcctc acggatttta tatccgattt tttcgttcct tagatccgtt tctacccgaa
    11041 cacccgactc ttccaaatcc gttacaagtt tctttatata ttcggcagat cggtctgtaa
    11101 ttgacagcaa cctcacctgt acaggggcga gccaaaccgg aaaggctccc gcataatgct
    11161 ctattaggat acctataaat ctttcgatgc tcccaaatac caccctatga atcattgccg
    11221 gacggtgttt ttccccgtca gcgcccacat aggtaagatc aaaacgctcg ggcatttgga
    11281 aatccaattg gattgtaccg cactgccaag ttctgcctat gctgtcttcg aggtgaaagt
    11341 cgatctttgg cccatagaag gcaccgtcac cttcgttaat aacatacttc atatttctct
    11401 cttccagggc ctctttgagg gcgtctattg caacatccca atcctcgtca gagcccatag
    11461 catcttcggg cttagtggaa agctccacat ggtagttaaa cccaaactgt ttgtagaaat
    11521 aatccacaag atcgattacg ccgacgattt catcctttac ctgttccggc agcatgaaga
    11581 tatgggcgtc atcttgggta aaacaacgaa ctctcatcag accgtgcagt gtgcctgaca
    11641 gctcatgtct atgaacgagt cccagctctc ccatcctcaa aggtaagtcc ctatagctaa
    11701 acattttcct cttatacagg atcatgctcc ccggacagtt catgggcttt accgcataat
    11761 cagcttcgtc tatgttggta aagtacatat tctcctgata atggtcccag tgcccggaac
    11821 gtatccacag atccctactt aagataatgg gagttcttat ctcctcatat ccccacttaa
    11881 catgttcctt acgccaaaat tcttccaatg tattccttaa aaccatgccc tttggatgga
    11941 aaaaaggaaa tcccggtccc tcttcatgaa tgctgaagag atccaactcc cttcccagct
    12001 tgcgatggtc ccttctcttg gcctcttcca agcgattaat atactcatcg agttgacttt
    12061 tcttcggaaa tgaaacccca tagatacgtt gaagcatctt gttgctttcc tcgcctctcc
    12121 aatatgctcc tgccaaattc agaagcttaa aggattttat cttacccgtg cttgggacat
    12181 gaggaccggc gcacagatcc acaaactccc cttgtctgta aaaggatatt acggaatcct
    12241 ccggtagatc ctcaatgagt tcgatcttgt aggattcatc tttttctgct acaaaatcta
    12301 tagcttcctc ccttggaagt tcaaatctct caagttccaa attctccttt attatctttg
    12361 tcatttcgga ttcaatgaac tctaaatcct cgggagtaaa gggtctttgc gtgtcgaagt
    12421 cgtaatagaa gccgttgtct atagaaggtc cgatagccag ctttatattg ggaaataacc
    12481 tcttaacagc ctgggccatt acatgagagg tggtatgcca gaaagccttt ttaccctcta
    12541 tatcctcaaa ggtcaagata ttgagttcac agtcctcatg caatggatgc atcaggtcca
    12601 cagtttgacc gtccacctga cccgctactg ccgccctggc aaggcccttg ctgatatccg
    12661 tggcaacctc gcccacggta ataccatttc tatattcctt ggaggatcca tcctttaaag
    12721 taattttaat catactaatt catctcctta tgatgcaata tgtcctttta ataaatccca
    12781 tatccgggat ttgcccgtac aataacccct gaagttaaat aaaacgtggt gggttttcat
    12841 cacccggccg cgagccagat gtggcatata tacaaaaatc cccgtccttc aaaggacggg
    12901 gataacccgc gattccaccc ttgttgattt ttagccatat agcggttccg cccaagaagt
    12961 atcattatag ggcttaccta ttgggtaata accgtactaa ataattagct caaaatccgg
    13021 ctctatagtc tgtaaaggga ccatccattc gctccggagt ggtattcaat cgaacctgtt
    13081 ggggaatgct ttcagcccgg gacattccct ctcttgacac cggtttccga tctactggtc
    13141 tctttccttg cgaattatta tcttatttaa taattataat tataacgcga taaaaagtca
    13201 ataatcttgt ttaaaatata aagcaaatag ctctatggtt attgggagct ctctttcaga
    13261 ctgttatgta tttccccata gacaagcccg gatatggaat catcaatccc cctgcccgtc
    13321 catatttcaa cggccttgat gccttggccc actaacattc ctattcctcc aacagttata
    13381 ctcccggatt ttctcgcctc ttttaagagc gtagtctcca aagggttata cactatgtcg
    13441 catactacgg tttccggact gaatgaatat ccggacagag gactgttttg cacattaggc
    13501 cacatgccca agggcgttgt attaattatt atatcgattg ttgccggtgc ctcttctaca
    13561 acggtggata gatcacggcc tgcgtatctg ttgatatcct cggccagttg ttctcccctg
    13621 gccgcattcc ggtttaatat gaggatttcc tttgggctgt cttccgctaa agtgattgct
    13681 atggccctgg cagatccccc ggctccaatt atcagaatcc ttcggccatg gcacctaatg
    13741 tttcgatttt ttaaggagct tagaaatccc aatccatcgg tattatatcc aatcatcctt
    13801 ccgtcttcta tcttcacagt atttacggcg ccgatagcaa tcgccatagg atctatttca
    13861 tttagatagt ccattacctc tatcttatag ggtatagtta cattaaaccc tgaaaaatta
    13921 agggagcgca atccccctac cgcactttgc aaatccctat gggatatatc aaaagctaag
    13981 taaaccatgt ctaatcccag cttatcatag atactgttat gcaacttggg ggacaggctg
    14041 tgggcaatcg gatgcccaat aacggcggca taggatgtct ttggcgagat gatcatccca
    14101 actcacctct ttttgccagt accctttcca aaaatcgatt gaatttggta ccccaaaatt
    14161 ccctggtgga tataggattg actaatattg tataaaggcc cgtgaggtta ccaccccaga
    14221 tatcggtaaa tatctgatcc cctatcatta tcgtgttttg aggtcttccg tctatatagc
    14281 cgagggcacg tttaaaggca gctcttaggg gctttccgcc tttgggaact gcggatatca
    14341 tcaactccgc cgctagtcta ttcaccctgg atgaagtgcc attggagaac aaacagactg
    14401 taaaccccag gatttggcat tgttttaacc aaccccttaa tctttcactt actctgtcgg
    14461 cattccaagg cgtgatagta ttatccacat cgataagtat gtttttatac cctctttcat
    14521 agagtccggc cagatcaatg gtaaaaacgc tatcgtggct ttcattgggt tttaaagctt
    14581 ctctcatgtt gtcccctttc gtttcatctg cgcctgcatg gtcttaccgc aattgggact
    14641 gccatgctaa tccggcatta gaattcttgt atgtataatt catcgcagcc gcttttaggt
    14701 gatggatacc atatattcct ttggcgacgg tatgcacaaa atatcaccca atcccaccaa
    14761 catccgggat ggtgattcca gcggtttcat ccccctcaaa cgcctgtaaa aacaatttta
    14821 gtataccata ttctgcccat gggaacaatc gttcaccctc tatggtagtc ccccacgtgt
    14881 tcggccccat cgtggattca cggacattct cggaaaattt acaataaaag acttttaacc
    14941 gataccaaaa ccgtcaccgc tgaattcttt gcctaatttc attatagctt ttttttcaat
    15001 cctggaaacg taagaccttg aaatgcccag gatctgggca atttccctct gggtcttatt
    15061 cttcccgtta atgagaccat aacgcatttc taggaccatt ttttctcgct gctttaagac
    15121 cgattgcatt ttgtcataaa gcctgtgtat ttggagtttt agctccacct catcgagtac
    15181 gctgtcggtt tcgctcccca atacatcaag aagggatatc tcatttccct ccttgtcgac
    15241 acctattgga tcttgcaatg agatttcccc tctaattttt ttatctgccc gtatcgtcat
    15301 caatatctca ttttcaatgc acctggccgc ataggtagcc aatctggtct ttctctcagg
    15361 gttaaaggtc gatatagcct taataagtcc aatggtgccg atagatataa ggtcgtctac
    15421 ctccttgccg gtgttggcat attttttaac tatatgagca actaacctca gattatgttc
    15481 gattagggtg ttcttcgagt cctccgatcc ctgagcacaa agtcttatat gatgggcctc
    15541 ttcttccggt ccgagaggtt gaggaaatga gtgggtaccc aatacatggg aagccaaaaa
    15601 atagatacac tttagaaaag aaacgattcc accggcggct ataagcattg gtcagcacct
    15661 ccaaaaaaga tgatggtctt atatcctatg atggagggta taaaaaagtg catgaccccc
    15721 tgctaatggg acataactaa actcgttacg ccagttcatt tccgacatgc tcagctggta
    15781 aggcaggtta acttaaggga tcctcagttt ttcggtatac cttgtgattt catcggctgt
    15841 gtccctgaag atcagggctg ctttttcccc ccctgatgat tcttcccgga ccaggatcac
    15901 tatggcatat ttaggcgtat ctgtcggaaa aaagcctgca aaccagccgt aatacccagc
    15961 atcggcgtct ccaaagatct gtggcgtgcc ggtctttccc gctgttccgc ctgctttcac
    16021 actatatgct ttaatgccgg tcccatattc agttacgtca cccatccaag ccctcaattc
    16081 cctggcagtg ccggacttta taagcctttc ccctgtctgt tcttcatata tgttttccac
    16141 cctatagccg gactgatctg ttatttctct gatcagggac aggtcctttt ttataccgtc
    16201 gctggctatg atgctcatca tttgggctat ctgaatcggc gtgacctcta cctttccctg
    16261 tcccaatgcc aaatttccaa ttcccgcccc ggcatattcg accttttggg gtagactccc
    16321 tctttcgtta tggatcccta tgtccagcgg ttgtcccagt cccatttttt gggccatttt
    16381 tattatagaa tcccccccta ctcggcttgc aataccaata aatgcgtcgt tacaggaatg
    16441 tgcgaaagcc tgcctcatac ttatatcgcc gagtcctccc ctctccgaat ggcagctata
    16501 cctgacgctg ttgatggtaa tcccgccctg gcatatatgc atagtttccc cgttacagat
    16561 accattctct atccccgcag tagccacaat aattttgaac agggaacccg gcgaaaaggc
    16621 tttcacgggt acggcccaaa ggctatcctt atgatttttg gccgtttcta tatcgttctg
    16681 atcaaaattt ggcctgctgg ctattgctaa gatatcgccg gtttcaactt ccactacaac
    16741 ccctccggct ttttgggcat ggctatccat ggccctttcc aatgcgctct ggacgtagta
    16801 atctatggtg aggcctatat cataatattc atcattcttg gacttattcc tatggactgt
    16861 acccgaaagg atccgccttt ttgcgtcggt catagcatac accgtcagaa catcatccga
    16921 gcggaggaat gcatcatatt ccttttctat ccccgattga ggagcattat cggacttttt
    16981 aaaatatccc attagatggc gggcaagagt aaaatcgtca tatctttggg tttcatagtc
    17041 tatcattaaa cccggatact ccccttgtcg gatcgacctt tcaattttgg ggtccgggtt
    17101 aagaatctcg catttaataa aggggctgcc tccgaggacg gaatcagata attcgtccaa
    17161 accaagcccg gtcatgggcc ctactatatc ataaaggctg tttctgtcat taaaatatgc
    17221 cggaaaaata agaagatatg cctttagcct gcctctggtg aggactatcc cgtttcgatc
    17281 caaaatatct cccctggcat gtttgacaac tatgggcaag atcccctgag aatgtgcttt
    17341 ctgtttgtaa tgaccaccca tgattaactg gatgtagagc aacctcataa ctaacgccgt
    17401 aaatagtatg gataaggcca atccgaaaaa aataaccctt ttatgggtta tggtttcctt
    17461 tttcatcgct acccctttgg ttaaatagaa tataactgtt ttctatatgt taaccggaag
    17521 ggcgatttct ataccttttg ttcccttctc agaatagagt atcccgtaat acccctatct
    17581 aagggcaaat ataccctctg ttgcgggtgg ggggccctat taataggcct gccttcttcg
    17641 tccaatattg tggtaacggt atagtccatg tagtcttccc cgggcatcat aatctcaagc
    17701 acatccccta cctcgaaggc atttctttgc tctacctcca tgagcccgcg ttctttatca
    17761 tatcccagca ccattccaac aaaatcgtag tttctaatat agctgctatc tgagtattga
    17821 tgatcttccc ccgccggttt tttgaaatag aaccccgtcg tatatgctct atggctggct
    17881 ttttgtatct cagcaatggc cttaggatcc catctgtagc catcctgatc ttctaggtaa
    17941 gcatccattt cccttcgata tgcctgtacc acagtcgcta cataatagct ggatttcatc
    18001 ctgccctcaa tcttaaatcc gtcaattcct gtacccatga gctggggtat gtgtcccaac
    18061 atacaaaggt ccttagagtt cattatataa gtccctctat cgtcttctag gaccggataa
    18121 tattccccgg gccttttctc ctcaacaata gaatacttcc atctgcaagg gtgcgcacat
    18181 tggcccctgt tggcatcacg gccggtgaaa acgttgctta gaagacatct gccggaatag
    18241 gatatgcaca tggcgccgtg aacaaaggtt tcaatctcca atgtatccgg tacattttct
    18301 cttatctggc cgatctcatt taaggacaac tcccttgcca aaataatgcg tttgaccccc
    18361 tgctcatgcc aaaacttggc gctacgccaa ttagtgttat ttgcttgggt actcatatga
    18421 attggcacgt tgggcatcgc ctcccgggca agggacaaaa tgccgggatc cgaaattatg
    18481 gctgcgtcta cacctatttt cgacaggaat ttcatgtatt catccatgtc cttgaaatcc
    18541 tcattatggg cgaagatatt catcgttaca tatacctttt tgtttataga atgagcatat
    18601 tccacagcct cattgatttc atccctttga aaattacctg caaaagcacg aagaccaaat
    18661 cgtttacccg ccatatatac ag
//
LOCUS       DASRNX010000177         3993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_406864,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000177 DASRNX010000000
VERSION     DASRNX010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406864"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1237
                     /locus_tag="VFD89_07105"
     CDS             59..1237
                     /locus_tag="VFD89_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZJ57993.1"
                     /translation="MNLTWILKPLVKVAAIKNSPKRIRAYIDGLGIKPADNISKDNTQ
                     DIHKESIRVCAVQEEIRLLKNHRQYTDRMHGFIKEAADRGAQLVTFPEENGTLILGML
                     PFMGTALKLLNRFTGTKKSEDRDKAQRIRPKRIPEKAQADAYKEGKAEKKNQGKARAP
                     LDIAKILAPLSSFLVDVFETTFSELAKAYGVYIMAGSMVLEDGGKVYNRAYLFGPEGN
                     IIGTQDKTHLVELEMGMGLSMGSNLSVYKTDIGRLAFPVCMDATYFETFKILKDQGAQ
                     VVIIPIANMEEYNYYFALRGIWPRVQESGVYGIKPALVGNLFGLKFTGKAGIYAPINM
                     TPEMDGVLGEAGTYNRDELIICDLDLKAIDEYRDEYFSDENLRFFKRHFPRVYANKEA
                     "
     gene            1337..1412
                     /locus_tag="VFD89_07110"
     tRNA            1337..1412
                     /locus_tag="VFD89_07110"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1370..1372,aa:Thr,seq:cgt)
     gene            1420..1496
                     /locus_tag="VFD89_07115"
     tRNA            1420..1496
                     /locus_tag="VFD89_07115"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1454..1456,aa:Pro,seq:cgg)
     gene            1715..2263
                     /gene="pgsA"
                     /locus_tag="VFD89_07120"
     CDS             1715..2263
                     /gene="pgsA"
                     /locus_tag="VFD89_07120"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZJ57994.1"
                     /translation="MNIPNILTSLRFCLVGVFIYIFYNPNIEGNLKWGIIIFIIAGIT
                     DVLDGYIARKYDMITKWGKLMDPLADKLMLIVVLVSLYTVDLVPLIVIIVVLAKELLM
                     VLGAIFIYRNRHTVVQSNFVGKASSAAFYVAIVALVLNIPYAFHILGAAVILTLVALI
                     QYGVLNLRKPQGGTKSNLDNIS"
     misc_binding    2316..2554
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2631..2843
                     /locus_tag="VFD89_07125"
     CDS             2631..2843
                     /locus_tag="VFD89_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ57995.1"
                     /translation="MPDRVLENLQRINPVGVIALIAAGFVAYGAKFIATVILRVPDDR
                     VFKVIALLKVLGLIIGLFGLYMIARK"
     gene            2960..>3993
                     /locus_tag="VFD89_07130"
     CDS             2960..>3993
                     /locus_tag="VFD89_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZJ57996.1"
                     /translation="MDEGKTIPKIYDPNRTEDRIYKEWMEGGYFKGKVDKDKEPYCIV
                     IPPPNITGQLHMGHALDNTIQDILIRWKRMQGYSALWMPGTDHASIATEVRIVEEMAE
                     EGLTKNDIGRDGFLDRAWNWKEKYGGRIVDQLKKLGSSPDWSRERFTMDEGCSHAVKE
                     AFIRLYDKGLIYQGDRIINWCPECKTALSDAEVEHQEQGGHFWHILYPIKDSDEKLTI
                     ATTRPETMLGDTAVAVHPGDERYGHLIGKTVILPLMDREIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGLRHDLEQLRVMDDAGVMNEYAGKYKGLDRYDARERIVNDLKDQGL
                     LAKVEDHSHNV"
BASE COUNT         1170 a          774 c         1041 g         1008 t
ORIGIN      
        1 gcccgtgtaa aacccgtacg ccttttaaaa aaagccatag tttaagggag ggacagggat
       61 gaatcttacc tggatattga aaccgttagt taaggttgcc gctataaaaa acagccccaa
      121 aaggatcagg gcctatatcg atggtttggg cataaaaccc gcagataata tctcgaaaga
      181 taatacccag gatatccata aagaatccat aagggtgtgt gctgttcaag aggagatcag
      241 gttacttaag aaccataggc aatatacaga cagaatgcac ggcttcatca aggaggctgc
      301 ggatcgagga gcccagcttg tgacctttcc cgaggagaac ggcaccctga tactggggat
      361 gctacccttt atgggaaccg ctttgaagct tctaaacagg tttaccggca ctaaaaagtc
      421 ggaagatcgg gataaggcgc aaagaattag gcctaagcgg attccagaaa aggcgcaagc
      481 ggatgcatat aaagagggta aagcagaaaa gaagaatcag gggaaagccc gagccccgct
      541 ggatatagcc aaaatattgg cccctttatc ttccttttta gtggacgtct ttgagactac
      601 cttttcggaa ctggccaagg catatggggt ctacattatg gccggtagta tggtgctgga
      661 agacggcggt aaggtctaca acagggccta cctcttcggg ccggagggga atataatcgg
      721 tacccaggat aaaacccatc tggtggagct ggaaatgggc atgggcctca gcatgggctc
      781 aaatctttca gtgtataaga ccgatatcgg cagactcgcc tttcccgtat gtatggatgc
      841 aacgtatttt gaaaccttca agatattgaa agaccaaggg gcccaggttg ttattattcc
      901 catcgccaat atggaggagt acaattacta ctttgccctt aggggcattt ggcctagggt
      961 gcaagagagc ggggtatacg ggattaaacc cgccctggtc ggaaaccttt ttgggctgaa
     1021 atttacaggc aaagcgggga tatatgcgcc catcaatatg actcccgaaa tggacggggt
     1081 attaggagaa gccgggacct ataaccgaga tgagctaatc atctgtgacc ttgacctaaa
     1141 ggccatcgat gaatacaggg acgaatactt ttcagatgaa aaccttaggt tctttaaaag
     1201 acattttccc cgggtgtatg cgaataagga agcatgatat cgaaaaagag gagcgaaagg
     1261 cttcataata gcacaaaaat aagatgacaa ttcgaaagac ctgtggtata atttataatt
     1321 gtattggaga ttgctagcca atatagctca gctggtagag caacggtttc gtaaaccgta
     1381 ggtcaggagt tcgagtctcc ttattggctc caaagatttc gggatgtagc gcagtttggt
     1441 agcgtgtctc gttcgggacg agaaggtcgc aggttcaaat cctgtcatcc cgaccattat
     1501 atctcaacga aattccttcg gatcctttgg aggaattttt acttataaga tgctattaaa
     1561 agcggtaggc caatggggac cctaaaaact atgttatggg gtttaattag tcccatctgg
     1621 cggtccattt ttcaaacatt tttatggtat agcatagaaa aatgacacgc tttgtgatag
     1681 atttgtcata tgttaaggaa gagggggcca taccatgaac attcccaata tattgacatc
     1741 tttaagattc tgtctggtcg gtgtctttat ctatattttt tataatccca acattgaagg
     1801 caatcttaaa tggggcatta taatattcat tatagcgggg attaccgatg tgctggacgg
     1861 atatatcgcc agaaagtatg atatgattac caaatgggga aagctgatgg acccgctggc
     1921 tgataagctg atgctaatag tagtccttgt tagcctatat acagttgatc ttgtgcccct
     1981 aatcgtcatt attgtagtgc tggcaaagga attattgatg gtcctaggcg ccatatttat
     2041 atacaggaac aggcataccg tcgttcagtc caattttgtt gggaaagcat ccagtgcagc
     2101 cttttatgta gccattgtag ccttggtcct caatatacca tatgcattcc acattttagg
     2161 tgctgcggtt atcctcaccc tggttgccct aatccaatac ggtgtactaa acttgagaaa
     2221 accccaggga ggaacaaaat caaatcttga caatatatcc taattgggta taatatcttc
     2281 tatatcatcc atggggaaat gggaataatt caatattatc ggtaatgagg aagaggagta
     2341 cattttttag gccgacagaa aaggggatcc gctggctgaa aggtccctcc cggttcgaca
     2401 aaatggaagg tcgcttctga acttttcaac tgaaatgatg agtaggttga aacgctaatc
     2461 ccgttaagga tttcgagtgt ttgtctaaac ccatatattt ttgtgggtat aagatgaaaa
     2521 tcaaggtggt accacgaagt cttgcgtcct tgtcatagga tgtagggctt ttttatattc
     2581 tattggtgtt tgtcgtgttt atcgtaaaac ctacagaaag gttgttgttt atgccggata
     2641 gagtattgga gaatctgcaa agaataaacc ctgtaggcgt tatagccctt atagcggccg
     2701 ggtttgtcgc ctacggggca aagtttatag cgacggttat actccgggta cccgacgaca
     2761 gggtatttaa ggttatagcc ctgctaaagg tcctggggct aattataggg ctctttggac
     2821 tgtacatgat tgcgaggaaa taagacatta ctaaaccaaa atcgcggttg aatctaccgg
     2881 taatagctat tggacgccca gttacatcga atgggtttag aatatttgta gagaaagcat
     2941 atgggaagga gaaatagaaa tggacgaagg aaagactatc cctaagatat atgatcccaa
     3001 tcggacggag gataggatat ataaggaatg gatggagggc gggtacttca aggggaaggt
     3061 agacaaggat aaggagccct attgtatcgt gattccgccg ccaaacataa cgggacagct
     3121 tcacatgggg catgccctcg ataataccat ccaggatatc ctcataaggt ggaaaaggat
     3181 gcagggctat tccgcattgt ggatgcccgg cacggaccat gccagcatag ccactgaagt
     3241 gagaatcgtt gaggagatgg ccgaagaagg gcttaccaag aacgatatag gcagggacgg
     3301 gtttttagat agagcctgga actggaagga aaagtacggc gggaggatag tagatcagct
     3361 gaagaagctg ggctcatctc cggattggtc cagagaacgc tttaccatgg acgagggatg
     3421 cagccatgcc gttaaggagg cctttattag gctctacgat aagggtttaa tctatcaggg
     3481 ggacaggatt ataaattggt gtccggaatg taagaccgcc ctttccgatg ctgaggttga
     3541 acaccaggag cagggtggac atttctggca tatactctat cccataaagg acagcgatga
     3601 gaagctgacc atcgctacca cccggcccga gactatgctg ggggatactg cagtggccgt
     3661 tcacccaggt gacgagaggt acggacattt aatcggcaaa acggttatac ttcccctcat
     3721 ggatcgggag ataccgataa tcgccgatga atatgtagat atggaatttg gtaccggagt
     3781 ggttaagata acgcccgccc acgaccccaa cgactttgag gttggcctaa ggcatgactt
     3841 agagcagctt agggttatgg atgatgccgg ggtcatgaac gagtatgcgg gtaaatataa
     3901 gggactagac agatacgatg ccagggagcg gattgtaaat gatctaaaag atcagggact
     3961 attagccaag gtagaggatc acagccataa tgt
//
LOCUS       DASRNX010000178         5511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_143263,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000178 DASRNX010000000
VERSION     DASRNX010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143263"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..1070
                     /locus_tag="VFD89_07135"
     CDS             321..1070
                     /locus_tag="VFD89_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HZJ57997.1"
                     /translation="MNYTIQKQLNHRTIRAWKDKAVEADILNTLFDVANRTASSVGMQ
                     SYSIIRVTDPEKRDRISKVCNQAYVKDVPELLIFLVDIFRNSQIAMEQGQSSPNKSTM
                     DKFFQGFTDGAIACQNLVNAAESFGLGTVYFGSILNDPEVVIDVLGLPKLTFPVIGLG
                     IGYPDEDPQLKPRMDIDLKVFENEYIIRDNYLKAIKEYDREMKTYYDLRDVSKPLDEF
                     SKQVVSFLGGDNPKRSRILDIIEKQGFILKR"
     gene            complement(1285..2058)
                     /locus_tag="VFD89_07140"
     CDS             complement(1285..2058)
                     /locus_tag="VFD89_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZJ57998.1"
                     /translation="MKNKVILILVDGMRPDGLLECENPYVPHLLESSCTFMDSQTIMP
                     SVTLPCHMSLFHSVPADRHGILTNTWVPQVRPVKGIFDVLAQNNKKSASFYNWEELRD
                     LGKPGTLTKSLYLALGDHENSDNILTDEAIKLAEESEPDFIFLYLGETDEIGHKHGWM
                     GKEYKDTVNNAVSCIERVIDATKGAYTFIITADHGGHDRSHGSDMPEDMTIPIIIHGK
                     GIPENIAGSPTILDIAPTVTHILGIAPDRDWEGISILGR"
     gene            complement(2227..2314)
                     /locus_tag="VFD89_07145"
     tRNA            complement(2227..2314)
                     /locus_tag="VFD89_07145"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2278..2280),aa:Ser,seq:cga)
     gene            complement(2421..2867)
                     /gene="tadA"
                     /locus_tag="VFD89_07150"
     CDS             complement(2421..2867)
                     /gene="tadA"
                     /locus_tag="VFD89_07150"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HZJ57999.1"
                     /translation="MFMREALKEAQKAYDLGEVPVGAVIVRNNKIIARGHNLREKDQN
                     PVLHAEIIAIQKAALEVKSWRLTGCEIYVTIEPCPMCAGAMVQGRLERLVFGAKDPKS
                     GCAGSLYDLVRDQRFNHRLEVVEGILGNECAQIIKDFFRRKRDQRL"
     gene            3203..4480
                     /locus_tag="VFD89_07155"
     CDS             3203..4480
                     /locus_tag="VFD89_07155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HZJ58000.1"
                     /translation="MFIQSEYTATDELSAIVKEGGIVIITRRAILKKIDPELMKTMSA
                     MSEQTVSIILHSHGGCDNRCQEILDKLGGEVKYEYPLIDSFSVEISSSKLMELASARY
                     VRYIAADVSVNAQMDIASQEIKSNVLNKSGYKGKGIGIAIIDTGIYPHADFLRPGNRI
                     KEFKDFVDKKDSPYDDNGHGSFVAGVAAGNGYLSRGKYQGIAPEADIIALKALNKDGS
                     GNTSDILAAIQWVADHQKENNIKVLSMSLGTDANRLSGNDSMVRAVEALWDRDITVVV
                     AAGNSGPKPSTITSPGISPKVITVGAVDDKRTPNISDDTIAEFSSRGPVGIRIKPDVT
                     APGVKVVSVNSNKDYKSGQRNITSTVPYTTMSGTSVATPMVSGSAAVLLQKYPSWTPR
                     QVKGALMDNAVPIVKNNNAEGRGLIDLERVTRD"
     gene            complement(4610..>5511)
                     /locus_tag="VFD89_07160"
     CDS             complement(4610..>5511)
                     /locus_tag="VFD89_07160"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HZJ58001.1"
                     /translation="YVLNKKFNLDIKTLSKANFYIFVPVFVFTNLYATHIPPEVFKVF
                     LFAVLVLIINVLIGVLISAITDHDIGKKYAFLNSIMFYNSGNVGLPLITLVFSSAPYI
                     IGGKTPYLDVAVTAQITVLAIQNITTNTFGFFNAGKASLHWRDSVKRIFGMPVIYMIP
                     AAFILKLVPYDLTRIPVWIGLEHIRNGMISLALITLGIQLSKTEFHVKNRDAYTAALI
                     RLVGGPFIAFILIKIMGFSGAIAQALMISTSVPTAVNTALIAVECDNHAEFSSQTVLI
                     STIFSAITLSLVIYLSRIIFPVA"
BASE COUNT         1678 a         1190 c         1136 g         1507 t
ORIGIN      
        1 tagtaaactc tggcttagta taatattaca ataatccggg gatcattgca cggttttttc
       61 gcacttttgc cccaaaattc tgcggactat cgggaatcaa cttgcgaccc ccaggtaccc
      121 atagtatact atattagggg cataataacc cggcggtcac caaaatttgc ttttgaaggg
      181 tttaaaaagc tgccggttca aatacgcaac ctatgggaat agacatctat gctgtaattt
      241 ttaataacgg tagtctttga cacactttta acaccgggtg ctttggttgc cttaactaat
      301 aaagcaagga gtggaagata ttgaactaca caatacaaaa acaattaaat cataggacta
      361 ttagggcatg gaaggacaag gctgttgaag ctgatatctt gaataccctt ttcgatgtgg
      421 ctaatcgtac tgccagcagc gtcgggatgc aatcctacag cattatacgg gttactgatc
      481 ccgaaaaaag ggacagaatc agtaaggtat gtaatcaggc ctatgtaaag gatgtccccg
      541 aacttttaat attccttgtg gatatattcc gtaattccca gatagcgatg gagcagggcc
      601 aatcaagtcc taacaaaagc actatggata agttcttcca gggctttacc gacggagcaa
      661 tcgcctgcca aaaccttgta aatgcggcgg aatcttttgg tctgggcaca gtctatttcg
      721 ggagtatact aaatgacccc gaagtggtta tcgatgtact ggggcttcct aaactaacat
      781 ttcctgttat aggcctggga attggctatc ctgacgagga tccgcagcta aaacctcgga
      841 tggatataga cctaaaggta tttgaaaacg agtatataat ccgggacaac tatctaaagg
      901 ccataaagga atatgacagg gaaatgaaga cctattatga tctaagggat gtctcaaagc
      961 ccctggatga gttcagtaaa caggttgttt ctttcctagg cggcgataac cctaaaagaa
     1021 gcaggatact tgatataatc gaaaagcagg gcttcatttt aaaaagatag agctttaaag
     1081 gatctagggc ccctttctct tctacccccc tgtaactaca taatggttat tatccggaag
     1141 gtattgcggg tctactttaa ccactatctc tggtgtcata atgatcatgc tgacgccaag
     1201 gatagtggtt aattttggta caacctgctt tacgggtgat gattgtccta tcaagaccca
     1261 caggtcacat ttcccccgcc caaattaacg acccaatata cttatgccct cccaatccct
     1321 atcgggggct atacctagaa tatgggttac agttggggct atatctaaga tagtaggaga
     1381 tccggctata ttttccggga tcccctttcc atggataatt atggggatgg tcatatcctc
     1441 aggcatatca ctgccgtggc tcctgtcgtg gccgccatgg tcagcggtaa tgataaaggt
     1501 gtaggcgccc tttgtggcat caatgaccct ttctatacag gaaaccgcat tgttgacagt
     1561 gtctttatac tccttaccca tccatccatg cttgtgtcca atttcgtcgg tttcaccaag
     1621 atacaggaag ataaaatcgg gttccgattc ttcggccagt tttatggcct cgtccgttaa
     1681 aatattgtcg ctattttcat ggtcacccag ggccagataa aggcttttgg tcagggtgcc
     1741 gggtttgccc aaatccctta actcttccca attataaaag gaggcggatt ttttattgtt
     1801 ctgagccagc acatcaaata ttcccttgac ggggcgcacc tggggaaccc aggtattggt
     1861 tagaatacca tgccggtctg cagggacgct atggaacaag gacatatggc aaggcagggt
     1921 caccgagggc attatagttt gggaatccat aaaagtgcag gaactttcta gaagatgggg
     1981 tacatatgga ttttcgcatt ccagtaaacc gtcggggcgc atgccgtcta ctaatattaa
     2041 aatcaccttg tttttcaatg taatatcctc aactttctat accattttta cggattgttt
     2101 tacaatacag ctttattatc ccttacagaa aagtcatata ggttttccat tgcactaccg
     2161 ctaaaagaaa acctgtgaaa ataaaaacga ccccccatat aaacagagaa gtcgttacat
     2221 cacagatggc ggagggttag ggattcgaac cctagatacc ttgcggtaca ctggttttcg
     2281 agaccagctc cttcagcctc tcggacaacc ctccatattt atatgtccct tatgataaat
     2341 ccttatatga aatatcttaa aaaggtaata tctatacaag gtatgctata caaaaagttt
     2401 atttttaaag gtatcagctt ctaaagtctc tgatctcttt ttcggcgaaa aaaatcctta
     2461 attatctgcg cacattcatt cccaaggatc ccttctacta cctctaatct atggttaaat
     2521 cgttgatccc gaactaaatc gtagagactt cctgcacatc cggatttagg gtccttggct
     2581 ccaaatacga gtctttccaa cctcccctga accatagccc cagcgcacat tggacacggt
     2641 tcaatggtaa cataaatctc acaacccgtc aatctccagc ttttaacttc cagagccgct
     2701 ttttgtatcg ctataatttc tgcatgaagg acaggatttt ggtccttctc ccttaggtta
     2761 tgccctcttg caattatttt gttattcctc actattacag cgcctacagg tacttcaccc
     2821 aaatcataag ccttctgggc ctcctttaag gcctccctca taaacatctc atacaatgac
     2881 ttcacatcct aaaccataag atgggataca gtcgatatta tggcatattt tccacgcaat
     2941 atcaataatg aagttttaaa atacgattca ataggttaag tgccaatttt tatgatataa
     3001 agtttctgaa gcgcctactt actaggcgtt tttgatagct tctaatcgtg tcacaattta
     3061 atcccccctc atattatata tcagagatat ctaccaggtt tttatcaggc taaaaacccc
     3121 catgaaaggg cctagcctaa aatcgatatc ttacaaattt tataaataga acaatttgtt
     3181 tgccttctta gaatcaaaca gattgtttat ccaatcagaa tatacagcga cagatgaatt
     3241 atcagcaata gtaaaagagg ggggaatagt tataattaca agaagagcaa tactaaaaaa
     3301 gatagatccg gaactaatga aaaccatgag cgccatgtct gaacaaactg tctctataat
     3361 actacattcc catggtggct gtgataatag atgtcaagag attttagata agcttggcgg
     3421 tgaggttaag tatgaatatc cccttattga cagttttagc gtggagatat cctcgtccaa
     3481 acttatggaa ttagcttcag ccagatatgt aagatatata gcggctgatg tctctgtcaa
     3541 tgcccagatg gatatcgctt cacaagagat aaaatccaat gtgctcaata aatcaggata
     3601 taaaggcaag ggtattggta tagcaattat agatacggga atttatcccc atgccgactt
     3661 tttaagaccg ggcaatagaa taaaggaatt caaagatttc gtggataaaa aggattctcc
     3721 ctacgacgac aacggccatg gcagttttgt agccggtgtc gcggcgggga atggttacct
     3781 ttcgcgggga aagtaccaag ggatagcacc tgaggcggat attatagcct taaaggcttt
     3841 aaacaaggac ggaagcggca atacttccga tatcttagcc gctatacaat gggtcgctga
     3901 tcatcaaaag gaaaataaca tcaaggtgct atccatgtcc ctagggactg atgcaaacag
     3961 gctatccggc aatgactcca tggtaagggc ggtagaagcc ctatgggata gggatataac
     4021 tgttgtggtt gctgccggaa attcaggccc aaagccttcc acaattacct cccccggtat
     4081 cagcccaaag gtaatcaccg taggtgccgt ggatgacaaa agaaccccaa acatatccga
     4141 tgataccata gcggaatttt ccagtagggg tcctgtgggc atacgcatca aacccgacgt
     4201 aactgcaccg ggcgtaaagg tggtttccgt taatagcaat aaggactata aatctggaca
     4261 gagaaatatt acctctacgg tgccatatac cactatgtcg ggtacatcgg tagctactcc
     4321 aatggtgtcg ggctcagcgg cagtattatt gcaaaaatat ccatcatgga caccgaggca
     4381 agtgaagggt gccctaatgg ataacgccgt accaattgta aaaaacaaca atgcggaagg
     4441 acgcggatta atcgatctag aaagagttac cagagattag ttcatgctaa atagaagggc
     4501 tgccccctct tttaataata tattgaaaag gggtggcctt tttatatcca ctggaaaaat
     4561 gctacctgcg ctatgggatc ggctaagcca tacaatccca tgtcctgccc taagctacgg
     4621 gaaaaattat cctcgataga tagattacaa gggatagggt tatggcgctg aaaatagtag
     4681 atattaatac ggtttgtgaa gaaaattcgg catggttatc gcattctacg gcaattaagg
     4741 cagtatttac agccgtgggg actgaagttg atatcatcaa tgcctgggcg attgccccac
     4801 taaaacccat gatcttaata agaataaaag caataaaggg tccccccaca agcctaatca
     4861 gagccgcggt ataggcatcc ctattcttaa catggaactc cgtctttgag agctgaatcc
     4921 ccagagtaat aagggcaagg gatatcatcc catttcttat atgttccagc ccaatccata
     4981 ccgggatccg ggttaaatca tagggaacga gcttaaggat aaatgccgct ggtatcatat
     5041 atataaccgg cataccaaat attctcttta ctgaatccct ccaatgaagg ctagccttac
     5101 cagcattaaa aaaaccaaag gtattggtgg taatattttg aatcgctaac accgtaatct
     5161 gagcagttac ggctacgtct aaatagggtg tctttccccc tattatataa ggtgcactgc
     5221 taaagaccaa ggtaattaag ggaagtccca catttcccga attatagaac ataatagaat
     5281 ttaaaaaagc atattttttc ccgatgtcat ggtccgtaat agcggatatc aaaacgccta
     5341 tcaacacgtt tattatcaaa accaataccg caaaaagaaa caccttaaac acttccggag
     5401 gaatatgggt agcatacaaa ttggtaaaca caaagacggg aacaaatata taaaaatttg
     5461 ccttgctcag agtctttata tccagattaa atttcttatt cagcacatat c
//
LOCUS       DASRNX010000179         7378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_193713,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000179 DASRNX010000000
VERSION     DASRNX010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193713"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFD89_07165"
     CDS             <1..894
                     /locus_tag="VFD89_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZJ58002.1"
                     /translation="IMVLMYHGIGDGEDVWVRTPENFRKDLKTLYDNGYRAIGMKDYI
                     EGNINTPPGTTPVVITFDDGLQGQFNVLGDGDEFIVDPDSAVGILEDFNKEHPDFGLK
                     ATFYVFYPIPFRQEKWISKKYEYLTEKGMEIGSHAYSHENLRMDMNTKEPRDAQFIQE
                     ALGKNVKATKEILPDYEVDSLALPYGATPKDEDVYKYVVKGSFEGVEYHNKAILLVGA
                     NPARPSYHKDTNMAKIPRIRASEMDTEGVGMYDWLDYFERHPEERYISDGDPKTISFP
                     KEMEEHIDADRIGDKVIRTYE"
     gene            1056..1475
                     /locus_tag="VFD89_07170"
     CDS             1056..1475
                     /locus_tag="VFD89_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HZJ58003.1"
                     /translation="MFEITVFFIVSELLDSHLTVIVFSYISQKARHRIGSAGTTENLD
                     MGKRKVSLKSIFPYFIIFFVAAAIINSLNFIPIGMGHVVNRTSKFIMIMALGAIGLKT
                     DLKEIKNSGLFPMLHGFIISAIVVVVSIAVQMALGQV"
     gene            1605..2174
                     /locus_tag="VFD89_07175"
     CDS             1605..2174
                     /locus_tag="VFD89_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HZJ58004.1"
                     /translation="MINKTGTKGLILISIFASLTAIGAFIKIPIPYVPITLQLLFCAY
                     SGILLGARWGLYSQLLYLAIGLIGIPIFTNGGGPSYILQPTFGYLVGFALCSYIIGRF
                     TENLRELTFHRVLGAVLAGMVGLYLCGLTHLYIIMNFYMQKPSSIQAVIGAGLIPFIF
                     TDLLSAIIASLSSVHILPILRRTGLLLKN"
     gene            complement(2211..2438)
                     /locus_tag="VFD89_07180"
     CDS             complement(2211..2438)
                     /locus_tag="VFD89_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZJ58005.1"
                     /translation="MKTVNVKLTLAENVKNFVNIVSKYPYEIDLRSGRHVIDAKSILG
                     IFSLDLSKPIIVEIYSDDCDDLLEEIKDFTI"
     gene            2766..3749
                     /locus_tag="VFD89_07185"
     CDS             2766..3749
                     /locus_tag="VFD89_07185"
                     /inference="COORDINATES: protein motif:HMM:NF021504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HZJ58006.1"
                     /translation="MKIGIPRALSYFDYFPLWQTFFKGLGFETIISPTTNKDILNKGV
                     SLCVDDACLPVKLFHGHVADLMGRVDAIFIPRLVSISPGEFICPKFIGLPEMIKNSIK
                     GLPPLLILNYDLHKRKKDHTKAFYDLGRQLGMDKSKTIRAYKEALERQYTYDMVLEAG
                     QSPLSILHPKERWDDVTHSRGTIGVIGHPYLLYDRYLSMDMIKKIYDKGYNVKVPANV
                     SDERIEDRLKDMPKKLFWTYGKRILGSGMDWLGDTEIDGIIFLSSFGCGIDSFTEELL
                     RRFNARGRRLPYAEFILDEHSGQAGFETRLKAFLDMIEWRDNSGSYLSSHG"
     gene            3721..4836
                     /locus_tag="VFD89_07190"
     CDS             3721..4836
                     /locus_tag="VFD89_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HZJ58007.1"
                     /translation="MVVTFPHMGKMYIPIKALFDELGVDIVVPPKINKRTLEVGIKSC
                     PEMACLPLKISMGNYVESIKRGADTIVLSGSCGPCRFGYYGVVQKEILRDLGYDIDVV
                     ILDPPREGIKEFLNRIRKITGNSSLYNGLKALKKAFKVSEETERLLKSVLIKRCREVE
                     KGSADRILNEFEDRLREINGADSILACIESARLRLESIEIDTDKRPVKIGLVGEIYTL
                     IEPYVNLDVEKRLGNMGVEVHREMTVDNWVKYHLAPDFSHRRRQKRILDQAKDYLGLC
                     IGGHAWETVAHSLHYAREKMDGIIQVMPFGCMPEIVAESILPTLARDEGIPIMTLVVD
                     ELTGETGYQTRLEAFVDLLQFERSKSSDEFRKVLSGY"
     gene            4805..5800
                     /locus_tag="VFD89_07195"
     CDS             4805..5800
                     /locus_tag="VFD89_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HZJ58008.1"
                     /translation="MNLEKYYLGIDVGSVSINLVLMDEDQRIKEKLYVRTEGKPISIL
                     KKAMEDILRDRSNNVYISGVGVTGSGRDLAAVMVGADVVKNEITAHGVAVLSSIPQAR
                     TVIEIGGQDSKIILIRNGVVSDFAMNTVCAAGTGSFLDRQAARLNIPIEEFGKISLRS
                     KTPVRIAGRCAVFAESDMVHKQQMGCPPEDIVRGLCEALTRNYLSNVGKGKEILPKVV
                     FQGGVAANQGIKAAFEETLGYEVIIPQHYDVMGAVGVAILAQREVKANGRTLFRGMEN
                     IKQDFKARTFECTSCPNACEIIEFKAGDTILARWGDRCGKWAVKAGDKQRLRSLA"
     gene            5973..6170
                     /locus_tag="VFD89_07200"
     CDS             5973..6170
                     /locus_tag="VFD89_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58009.1"
                     /translation="MPRKPAPSHEQKRYVASVCPEYHPGHDGSLTSSIDEGDASCDAC
                     IHWNDGECVIFDDVLTGIDQE"
     gene            6248..6424
                     /locus_tag="VFD89_07205"
     CDS             6248..6424
                     /locus_tag="VFD89_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58010.1"
                     /translation="MDEIGKMEDYMGAYNPYTDKDVFTIDNKGLLMGDDQCVHLYYCK
                     SCHLIGYGSAPYIL"
     gene            6587..6762
                     /gene="ssrS"
                     /locus_tag="VFD89_07210"
     ncRNA           6587..6762
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFD89_07210"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            6885..6958
                     /locus_tag="VFD89_07215"
     tRNA            6885..6958
                     /locus_tag="VFD89_07215"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6917..6919,aa:Gln,seq:ctg)
     gene            6988..7062
                     /locus_tag="VFD89_07220"
     tRNA            6988..7062
                     /locus_tag="VFD89_07220"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7021..7023,aa:Glu,seq:ctc)
BASE COUNT         2209 a         1459 c         1817 g         1893 t
ORIGIN      
        1 ataatggtct taatgtacca tggaataggc gatggggagg acgtatgggt aaggacaccg
       61 gaaaacttcc gaaaggacct taagacccta tacgataacg gataccgtgc aatcgggatg
      121 aaggactata tagagggaaa cattaacact cccccgggca caacccccgt ggttataacc
      181 tttgacgacg gccttcaagg ccagttcaat gtcctagggg atggagatga atttattgta
      241 gaccctgaca gtgcggtggg cattttggaa gatttcaaca aggagcatcc ggattttggg
      301 ctcaaggcta ccttctacgt attctatccc atacccttta gacaggaaaa atggatatcc
      361 aaaaagtatg aatacttaac tgaaaagggt atggagatag gcagccatgc ctacagtcac
      421 gagaacctta ggatggatat gaacaccaaa gaacccaggg acgcacaatt tatacaggaa
      481 gccctaggaa agaatgtaaa ggcgaccaag gagattctgc cggactacga ggtagacagc
      541 ctggctctgc cttacggggc tactccaaag gacgaggatg tctataagta cgttgtaaag
      601 ggcagctttg aaggcgtgga atatcataac aaagccatat tattggttgg tgccaaccct
      661 gccagaccct cctaccataa ggataccaat atggccaaga tccccagaat tcgggccagc
      721 gaaatggaca cggaaggggt cgggatgtac gattggctgg attactttga aagacacccc
      781 gaggagaggt atataagcga cggggaccct aaaaccatat ccttccccaa ggagatggaa
      841 gagcatatag atgccgatag gataggggac aaggtcattc gaacctatga ataatcaaat
      901 cccaaaagtt ttgtaattgc tttgatatat acttgttttt atgatagatg atattgaatt
      961 tgcgcttgaa tttaatatca tctattttta tgtggttgag cctttgatcc ctaagctcct
     1021 cttccaccat catttttgag ataaccgtta ctccgatgtt tgagatcacc gtattcttta
     1081 tagtttccga actattggat tcccacttaa ccgttatcgt attctcatat atatcccaaa
     1141 aggcgaggca taggatagga tctgccggta ctacagaaaa tttggatatg ggcaaaagaa
     1201 aggtgtccct aaaatccatc tttccgtact ttatcatatt cttcgtagcg gcagccataa
     1261 taaacagtct aaattttatt cccattggga tggggcatgt agtaaacagg accagtaaat
     1321 ttatcatgat catggcctta ggagcaattg gactaaaaac ggatcttaag gagataaaaa
     1381 attccggact gtttcctatg ctccatggct ttataatatc cgccatagta gttgtggtat
     1441 caatagcggt acagatggct ttgggacagg tttaaggacg ataatagggc gccgagatga
     1501 attgtcaacc acagaataga ctgtggttga caatgcgaat aaaattatgg tacaattgca
     1561 ccggttttaa aattttataa caaatacagg aaatggagta ctatatgatt aataagaccg
     1621 ggactaaagg tttgatccta atctcaatat tcgcatctct gaccgccata ggcgccttta
     1681 tcaagatacc cataccctat gttcccatca ccctgcaact actcttttgt gcgtattccg
     1741 ggatactctt aggcgcaagg tggggattgt attcacagct tctgtacctc gctattggac
     1801 ttataggtat ccccatattt acaaacggag gcgggccctc ctatattctg cagcccacct
     1861 tcggctatct ggtggggttt gcactatgtt cctatataat tggcaggttt acagaaaatt
     1921 taagggaatt aacatttcat agggttttgg gcgcagtctt agccggcatg gtcgggctgt
     1981 acctgtgcgg actgacccat ctttatatca ttatgaattt ttacatgcaa aagccgtcgt
     2041 ccattcaggc ggtaattggg gcagggctta taccctttat cttcacggac cttctctctg
     2101 ccataatagc atcccttagc tccgtgcata tcttgcctat cttaaggaga actggactgt
     2161 tgctaaaaaa ttaggccaca aaaaaaccct cgaatcctcg agggtttttg ttaaatggta
     2221 aagtctttaa tttcttctag aagatcatcg caatcatcgg aataaatttc aacaatgatg
     2281 ggtttgctaa ggtcaaggct gaaaataccc agtatagact tcgcatcaat tacgtgtcgt
     2341 ccagagcgaa gatcgatttc ataaggatac ttgctgacga tattgacgaa gttctttaca
     2401 ttttcagcga gggtcagttt aacgtttacg gttttcataa gatcattcct cctaaataat
     2461 tattatatac ataagacatc ttccaatagc ggcggaatcg gggaataaca cgttagaata
     2521 aaagcatatg taacaaattc caccattttt agtatatacc atccggccca agtatcaata
     2581 cttttcactg gccatttttt gcccgccggg tcaaaaatcc catttgtaca ttttccagtt
     2641 taataatccg tccgcgagtc aataataaaa aggattgatg cattttttaa gaagatcaat
     2701 taatccatta gatagatacc caaactttta agattcaaaa catgcataat aaggaggtac
     2761 gacttttgaa aatcggaatc ccaagggctc tctcctattt tgattatttt cccctatggc
     2821 aaaccttttt taagggcctg ggctttgaaa ctattatatc ccctaccacc aacaaggata
     2881 tattaaataa gggggtatcc ctgtgcgtcg atgatgcatg ccttcctgta aagctgtttc
     2941 atggccatgt ggcagaccta atgggaaggg tggatgccat attcatacca agattggtca
     3001 gcatatcgcc gggagagttt atctgcccaa aattcatcgg acttccggag atgatcaaaa
     3061 attccataaa gggtctgcct ccgctgttga tccttaacta cgacctacac aagcgtaaaa
     3121 aggatcatac taaggccttc tatgatttgg gacggcagct gggcatggac aaaagcaaaa
     3181 ctatcagggc atataaagaa gccttggaga ggcaatatac ctatgatatg gtcctagagg
     3241 ccgggcaaag cccactgagc atcctccatc caaaggagag atgggacgat gtcacccatt
     3301 caagggggac tattggggta atcggacatc cctacctcct atacgacagg tacctttcca
     3361 tggatatgat taaaaaaatc tatgacaagg gatacaatgt aaaggtgcct gccaatgtct
     3421 cggatgagag gattgaggac aggctaaagg atatgcctaa gaagctattt tggacctacg
     3481 ggaagaggat actcggcagt gggatggact ggttagggga cactgaaata gacggcatta
     3541 tatttttatc aagctttggc tgcggcatag attcctttac agaggagctc cttcgaaggt
     3601 ttaacgctag ggggagaagg cttccctatg cagaatttat cttggacgag catagcggcc
     3661 aggcggggtt tgaaacaagg ctgaaagcct ttttagacat gatagaatgg agggataata
     3721 gtggtagtta cctttcctca catgggtaag atgtatattc ccatcaaggc attgttcgac
     3781 gagctggggg tggacattgt agtgccccct aagataaaca aaagaacctt ggaggtcgga
     3841 atcaaatcct gcccggagat ggcatgcctt cccttgaaga tatccatggg aaactatgta
     3901 gagagcataa aacggggcgc agacacaata gtgctgtcgg gaagctgcgg cccatgcagg
     3961 tttggctatt atggcgtggt acaaaaggag attctaaggg atttaggata cgatatagat
     4021 gttgtgattt tagatccccc cagagagggg ataaaagaat ttttaaacag gataagaaag
     4081 atcaccggga atagctcctt gtataacggg cttaaggccc taaaaaaggc ctttaaggtg
     4141 agtgaggaga cggaaaggct cctcaagtcg gtgcttataa aacgctgccg ggaagtagaa
     4201 aagggcagcg cagacagaat cttaaatgaa tttgaggata ggctcagaga aataaatggg
     4261 gcggatagta tcttagcctg cattgaaagc gctagattaa ggctggagag tattgagata
     4321 gatacggaca aaaggcccgt taaaatcgga ttggtggggg agatatacac cctcatagaa
     4381 ccctatgtga atctggacgt ggaaaaaagg cttggaaata tgggcgtaga ggtacatagg
     4441 gagatgacgg tggacaactg ggtgaaatat catctggccc cggattttag ccacaggcgg
     4501 aggcaaaagc gcatcctgga tcaggcaaag gattatctgg gcctgtgtat aggcggccat
     4561 gcctgggaaa cggtggctca tagcctgcat tacgccaggg aaaagatgga cggaatcata
     4621 caggttatgc cctttgggtg catgccggaa atagtcgcag agagcattct gcccaccctt
     4681 gcaagggacg aaggtatccc gataatgacc ttggtggtgg acgagctaac gggggagacg
     4741 ggttatcaga caaggcttga agcctttgta gaccttttgc agtttgaaag gagcaaaagt
     4801 agcgatgaat ttagaaaagt actatctggg tattgatgta ggctccgtca gcatcaatct
     4861 ggtcctgatg gatgaggatc aaaggattaa agaaaaacta tatgtacgca ccgagggaaa
     4921 acccatatcc atcctaaaga aggccatgga agatatatta agggacagga gcaataatgt
     4981 ctatataagc ggggtaggcg ttaccggcag cggaagggac ctggcggcgg ttatggtcgg
     5041 ggcggatgtg gtaaagaacg agataaccgc ccatggcgtt gcagttctca gttctatacc
     5101 ccaggcaagg accgttatcg agatcggggg ccaggattct aagattatcc tgattcgaaa
     5161 cggagtggtt tcagattttg caatgaatac ggtgtgtgca gccgggacgg gttcgtttct
     5221 cgacaggcag gcagccagac taaacatccc catcgaggag tttggcaaga tttccctaag
     5281 gtcaaaaacc cctgtcagga tagccggtag gtgtgcggtc ttcgcagaat cggacatggt
     5341 ccataaacaa cagatgggtt gtccccccga ggatatcgta agggggctgt gcgaagccct
     5401 tacaagaaat tacctgagca acgtgggaaa gggcaaggag attctcccaa aggtggtctt
     5461 tcaagggggc gtggctgcta accagggaat aaaagctgcc tttgaagaga ccttggggta
     5521 tgaggtgata atcccccagc actatgatgt aatgggcgcc gtgggggttg caatccttgc
     5581 tcaaagggag gtaaaagcta atggacgtac cctctttagg ggcatggaaa acatcaaaca
     5641 ggactttaag gccaggacct tcgaatgcac cagctgcccc aatgcctgcg agataatcga
     5701 atttaaggca ggggacacta ttttggcaag atggggggac aggtgcggca aatgggccgt
     5761 aaaagcaggg gacaaacaaa gacttcgttc actggcatga atatatggga ttaagggtga
     5821 aatactaaaa ccagcattaa aatattgatg tgctctgtaa gtgaattata gataaacagc
     5881 tccaactata aaatctatcg cctatagata aattcggttt ttgtgttgtt tatctgtacc
     5941 aaatatcact ctaaattaag gaggaggatt ttatgcccag aaaaccagct cccagccatg
     6001 aacaaaaaag gtacgtagcc tcggtatgcc ccgagtatca tccgggtcat gacggttctc
     6061 taaccagctc catagacgaa ggggatgcca gctgtgatgc ctgcattcac tggaatgatg
     6121 gagaatgcgt aatattcgat gatgttctca ccggaataga tcaagaatag gtatggggac
     6181 cagggtctgc cccatttgta acggcctgct ccaggtccat gaattatgcc ctaggtgtag
     6241 tagggagatg gacgagatag ggaagatgga agattatatg ggggcctata atccatatac
     6301 ggataaggac gtctttacca ttgacaataa agggctattg atgggtgatg accaatgcgt
     6361 tcacctgtat tattgtaaat cctgccacct aattgggtat ggttctgctc catatatcct
     6421 gtagatatgg ccaataggcc aacgttgatt gggttacgta aaccaaggga aaccgaaaaa
     6481 acgtgataca tctggggttt aagcgggata tggcgggaat ttggatataa ttatctattg
     6541 aaaatatgcc cgacttatac tatcatatag atacagcaaa agacggataa tcctgtgatg
     6601 tgcgtgaatc tattgttttt aacccacaga gttaatacgg gagtccgggt ttgaccctcg
     6661 gatgccgtgc cccttagggg gaaggcagaa ttgggcaata ggacacccac ctgccaagag
     6721 gcgggtataa aaatagtaga atgacggcat tatgggaata gtaatccccc attaatagtg
     6781 ggggattact attatcctgg gatttggcgt attttgggaa ataaaaaatt ctgttgacaa
     6841 taattgcccg tatgatataa tattatccgt tgacaaatga atattgcgga atggtgtaac
     6901 ggtagcacat gtgactctga ctcatagagc ctaggttcga atcctagttc cgcagccatt
     6961 gaacttgcac ccgcaagttc attttacgcc cccatggtca agcggtcaag actccgccct
     7021 ctcacggcgg catcaggggt tcgagtcccc ttgggggtac cagataaaag ccttatggtt
     7081 ttcaagacta agcgaaatca taaggtcttt tttatgtaaa ttttttgata ttatattcac
     7141 attgtgagat accgaataga gatggaatga caaaagaatt aaaagattga tataacaggc
     7201 tttccagata accctagata tgagaccaat catcgggaca gcactttatt tcttaaaaca
     7261 aaccgtttaa tcaataatta ttacatgatg tatgtcaaga ggatttacaa tcaaagtaaa
     7321 tcccttcagt tttattgaat tatgctggaa agtatagtaa aattggatat acaagcaa
//
LOCUS       DASRNX010000180        13392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_512894,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000180 DASRNX010000000
VERSION     DASRNX010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13392
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512894"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /locus_tag="VFD89_07225"
     CDS             <1..1565
                     /locus_tag="VFD89_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZJ58011.1"
                     /translation="LVLIVASFVGCGSSDNVKDPAPGKEVTPPAGDTDDNGDDTEDVT
                     PTLDTSKKIELVSWFAGDGPAGLDDILGPLNEKISEDLNATLKVNYLGWADYQTRYSL
                     LLNAQEECDLLYSANWLNARDYYRKGAFLDLNDYLTDYAPELYAFLDRWEDVSISGNI
                     YAIPNNAVRHNSGGITYREDLRKKHDLPLPDSIENIEAYFEGIKQNEPTIIPTSDRAV
                     MDAMFDASRFTWGKYDTIGAKFGANATGYDESKLAEVTYDWETSEFLEMLKLMKSWAD
                     KGFWSRSLLSAQNDSAEDFEAGKTAAIVSGQHTDKWSGYVTRHEEEGTEGWEVEFIPY
                     YNKYGISLLTSAEQDMTVIPIQSKDPARAVALVEKIFMDKEYFQLIQYGIEGEHYVVN
                     EEGQYEPGPKQEEYGVGGMGSWGWRNIDFYLDNPSVPAKVTAMHEIMDTFAPVSPSPF
                     SMDNSSVEAEAASLQTAIEQYLNPLKFGQHDNPEKGLEEFMKQAKIAGYDKVKEEFES
                     QWDVFLGEMGLK"
     gene            1660..4623
                     /locus_tag="VFD89_07230"
     CDS             1660..4623
                     /locus_tag="VFD89_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HZJ58012.1"
                     /translation="MNRMGNCLENGTSVYGKRPHDPLAVYLTPENFPVKADGITDDTD
                     AIQQAINEAQRRSGYGIVFMPEGNYRISKTIYISKGIRLIGYGKKRPVITLEENCPDF
                     QEADPEDKGQANYMVWFTDRLPKPGEPVRDAGAGTFYSAISNIDFVIQDGNPTAVALR
                     THYAQHSFISHVDIHIGQGKAGMFDVGNEMENVCFFGGEYGIYTTKTSPGWPFMMVDT
                     HFEGQRRAAIRTQEAGLTIVRMTARSVPVVIETDPNLYEKLYMEDSQLEDISDAALII
                     SNEFNTHTQINLRNVHCRNVKRIAKYRESGKIIEGSENIYSIETLIHGNHIDGLKIPA
                     EMKVTCHIKDLDSFPNPPKKDIPQTPGIETWVNLKELGAVGDGEADDTEIIKDIISKH
                     RVIYAPQGFYRVTDTIELRPDTVLIGLNPIATRFILKDNTEGFGGVGAPKALLEAPKG
                     GRNIVMGISLDTAGKNPRAVGCKWMAGADSYMNDVKFLGGHGGMNPEGGYVPSYNATR
                     TADANPEYKWDTQYWSLWVTDGGGGTFKDIWTASPYTAAGLYVSDTSTEGRIYAMSVE
                     HHVRNEVRFKNVSNWKVLALQLEEEVAEGPYCLPLEIQDCRDMVFANLYLFRVIWHTN
                     PYPYAIKTWNCKAVEFLNIHNYTQIKYTFDNTLFDVTTNTQIRPWEIARLYLDGKSCV
                     DGEDVTDTCVCSDATGDIRELADDFEFIDGICADSKGDIYFCDNRWKRIYRWSAGDNS
                     LALLTEIPYKPLALGFDTEDNLLVVVEYSPAKGATIHGELEIYAKPEDSRGTSHGYWY
                     SVNSTTKVIAIDPNNPDTSIESLDRVDIDSVKEVFKALHPANRWRDGHDFLEVSVNKP
                     SKCFVAPDGITIIPVCYDLMRATNLLEAYPNKPFYGVDEYNKKTAIFDVNSKGYLENP
                     RIFAEKGESNVAVDHGDNVYIPDGQVYIYSADGNLLDEIEVPTRPSGVIIAGEDKDTL
                     FIAARSSLYAVSIKQ"
     gene            4925..5680
                     /locus_tag="VFD89_07235"
     CDS             4925..5680
                     /locus_tag="VFD89_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZJ58013.1"
                     /translation="MRIFGGNLEKLSALGYRGMEGGEALLKGDVEDNLKRFHDLGLKV
                     LTVSADMEGLRQGNYDGIIEKAKQLGTDRATVWACAINSSFWNKEPVYDEVMRDIEAM
                     EKAAVKLDEEGICLRYHNHHQDFLVSFKGITCFDHLIYNTEKLSIELDVGWVTNGLQD
                     PSVVMERIKDRLDAIHVKDYLNGEYREKEGGWAPIFTTLGNGELKLLDALETAQGLGI
                     EWAVVEQDQMHNLSPMESLTASYLNMKETGIVL"
     gene            5943..7331
                     /locus_tag="VFD89_07240"
     CDS             5943..7331
                     /locus_tag="VFD89_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HZJ58014.1"
                     /translation="MKNRDKLVVGVLPVKRGFLSLEVAMEEKKKLFNAIGGIKDDFVT
                     LVDVDDVCEGGVLFKLDEVQAAVDKFKKADIDALFICHCDFGEEQALIKVAREFDVPV
                     LLWGNRDKFPNTFEKRGRDTQCGMFASTKVLSRNKITFSYIYNVDVETDEFMDGYLTF
                     LRTANVVKAMKRLKIAQIGLRPQQFMSVIASEGLMVEKFGIEVVPFSVPAIIRNVEEL
                     AAAKGSQVQEGIASFKERMDCSQMTEEQLAKIAALKITVKDMMEDSGCRAGAIECWSL
                     FSREIGVAPCMIVSELADLGLPLSCEMDLNGAITSVIADAVTLGESAVFFADLTIRHP
                     ENDNAELLWHCGPFPYSLKDPDKPARMINAQAIWELKKGDITILRCDDIDGEYKVIAS
                     EGKAVDGPETTGSYVWFESPCWKSLEEKFIFGPYIHHVAGVYGNYSRAIEEAARYLPI
                     KWDKVGTGPRSL"
     gene            7419..8273
                     /locus_tag="VFD89_07245"
     CDS             7419..8273
                     /locus_tag="VFD89_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HZJ58015.1"
                     /translation="MPIERTSVILKDAEKHGYGVAAFNIFNYETIAWAIQAAEEERIP
                     VIIQFYPGFDRFIPMEVVTAATKALAAKASIPVGLHLDHSNKYEDAIRGAECGFTSIM
                     IDGSTLDFDENADLTKRVVDTAHKMGVEVEAELGHVGSGNNIDDFTNSSHFTDPEDAI
                     RFIEITNADSLAVSVGNGHGHYVATPSLDFDRIQDIHDSISVPIVMHGGSDIPDDQIQ
                     RSIACGMSKFNIATEYNRSFYNAMKKCMEEDQGHGYMFRCLAQAENEVKEFLKAKLRV
                     LNPKSYRL"
     gene            8612..9712
                     /locus_tag="VFD89_07250"
     CDS             8612..9712
                     /locus_tag="VFD89_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HZJ58016.1"
                     /translation="MEKIRGVNLGNWLVLEKWMHPPLFHGVDAEDETDLCLLLSDEDK
                     RARLKAHRDSYITEYDFRYIKSIGMNIVRLPVPHFVFGDRPEFCEPYVPCIEYVDKAL
                     EWAKMTGLKLLIDLHTAPDSQNGFDNGGICGVCKWHTKEENIERTLEVLEMLAKRYKD
                     NDGLYGIQLLNEPVAPFMWEFVKERYPATDPERAKGSGGVPTEVLRDFYTRGYHRLRK
                     HLDEDKVIMFHDGFRLKEWEGFMQGPEYKNIMFDAHIYLAMTDHDMPETIRLSHYLKK
                     LEEYAEGLKHMRQFFPVIIGEWSLMHRITGMEDFSPAELRHSYSTVANAQLFTWESAT
                     DGWIFWSYKLLSESPGWSLRDCIQKGWFPERV"
     gene            9845..10444
                     /locus_tag="VFD89_07255"
     CDS             9845..10444
                     /locus_tag="VFD89_07255"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012849698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZJ58017.1"
                     /translation="MRFPQDIFYGIKDEEIIEVINSIPRETFIPKSLKGYAYTDTALP
                     IGSDQTISQPSLVAYMTQLLGLTGKERVLEIGTGSGYQTAILARLAQKVYTIEIIREL
                     HDKSKQILEEMGILNVCYRLGSGYEGWEEHAPFDGILVTAAPPKIPAKLIEQLKDNGR
                     MVVPVGARGQTQILKLLVKSGDTIMEEDKTYVRFVPMVE"
     gene            complement(10458..10931)
                     /locus_tag="VFD89_07260"
     CDS             complement(10458..10931)
                     /locus_tag="VFD89_07260"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZJ58018.1"
                     /translation="MGNVFYYDTPIGRLMIGGQDDNITYLLFEGNPVPRGYSIGETPI
                     LAEAARQLDEYFCGRRKEFDLRLEPMGTEFMNKVWTSLLKIPYGQTRSYKDIAESIGK
                     NKAYRAVGLANNRNPISIIIPCHRVVGSNGALVGYGGGLEIKTFLLNLEQENSSL"
     gene            11246..12289
                     /locus_tag="VFD89_07265"
     CDS             11246..12289
                     /locus_tag="VFD89_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HZJ58019.1"
                     /translation="MDGYKIIGYLADWADWTAEDVDAQKLTHINYAFATIDDGRVVAK
                     VLDRRLGKLHILKEIKAQNPHLKTLISVGGWGADGFSDAALTEESRELFADTAVKFMK
                     DYGFDGVDIDWEYPCSSAAGIVSRPEDKENFTLMLEELRQQLDREGEQDGRHYLLTIA
                     VGAGQYFIDGSEMGKIQKYLDFVNIMTYDYANGLDEHTGHHTHLFESKVTPGSSSDKS
                     VRLFMGQGIPASKLVLGVAFYGRAWSGVSGENNGLGMGGIPGAGGLDTSYTNLSSNLV
                     DKNGFDRYWDEDAKAPYLYNGDIFISYDDEESIGHKVDYIKETGLGGVMFWEYSSDKS
                     GALLKKLYEGLRS"
     gene            12961..13260
                     /locus_tag="VFD89_07270"
     CDS             12961..13260
                     /locus_tag="VFD89_07270"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HZJ58020.1"
                     /translation="MELKTLDSLGVGEKGIITAINGGRTATIRLCEMGFNRGSEVCIV
                     KNDIGPIIVGLAGSKIALGWGLANKIMVQQKEPGLQHAGAWGKGGMRLADDSISR"
     gene            13235..>13392
                     /locus_tag="VFD89_07275"
     CDS             13235..>13392
                     /locus_tag="VFD89_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015294839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HZJ58021.1"
                     /translation="MQTIALVGNPNSGKTTIFNALTGTNQHVGNWPGVTVEKKEGIVK
                     FSDNEYSV"
BASE COUNT         3861 a         2727 c         3446 g         3358 t
ORIGIN      
        1 ccttggttct catcgtcgca agttttgtcg gctgcggttc ttcggacaat gtcaaagatc
       61 ccgcccccgg taaagaggta acccctcccg ccggtgacac cgatgacaac ggtgatgata
      121 ccgaagatgt gactccaaca cttgacacat ccaaaaaaat agaattggta tcctggtttg
      181 ccggtgatgg acctgcaggc ctagatgata tccttgggcc tcttaacgag aaaatttccg
      241 aggatctaaa cgccacccta aaggttaatt atttaggttg ggctgactat caaaccagat
      301 actccctact gctaaatgcc caagaggaat gtgaccttct ctattctgcc aactggttaa
      361 atgccaggga ctattatcgc aagggtgctt tcttggatct aaacgattat ttgacggatt
      421 atgctcccga gctatacgct ttcttggata gatgggaaga cgtttcaatc agcggcaata
      481 tctacgctat tcccaacaat gccgttcgtc ataacagcgg cggtatcacc tatagggaag
      541 acttgcgtaa aaagcatgac ctcccgctgc ccgattctat agaaaatatc gaggcatact
      601 ttgaaggaat caagcaaaac gagcctacaa taatacccac ctcagatagg gctgttatgg
      661 atgccatgtt cgatgccagc cgtttcacct ggggtaagta tgacaccata ggcgcaaaat
      721 tcggtgcaaa tgcgacagga tatgatgagt ctaagcttgc agaggtaacc tatgattggg
      781 agacctccga attccttgag atgctaaagc tgatgaagag ctgggccgac aaaggattct
      841 ggagccgcag cctgttgtct gcacagaacg actccgccga ggactttgaa gccggaaaga
      901 ccgctgctat agtgagcgga cagcacactg acaagtggtc cggttatgtt acaagacatg
      961 aagaagaagg aactgagggc tgggaagttg agtttatccc ctattacaac aaatacggta
     1021 taagcttgct tacctcagca gaacaggata tgacggttat tccgatccag agcaaggatc
     1081 ccgcacgcgc ggttgccctt gtagagaaga tcttcatgga taaggaatac ttccaactga
     1141 ttcagtacgg tattgaaggc gaacactatg tggttaacga agaaggccaa tacgagcccg
     1201 gtccgaaaca agaggaatac ggagtaggcg gcatgggttc ttggggttgg agaaacatcg
     1261 atttctatct cgacaacccg tctgtacccg ccaaggtaac cgcaatgcat gagataatgg
     1321 atacctttgc tcccgtatca ccttcaccct tctcaatgga taacagcagc gttgaggctg
     1381 aagccgcatc attgcagact gccatagagc agtatctgaa tccgttgaag tttggtcagc
     1441 acgacaatcc tgaaaaggga ctcgaagagt ttatgaaaca ggctaagata gccggttacg
     1501 acaaggttaa ggaagaattc gagagtcaat gggatgtctt cttaggcgaa atgggcctta
     1561 agtaaatcct gttggtaaaa cttataataa ccctattaaa tatctcccct agtgttatac
     1621 taggggggaa tttaatattt ctaggaaggt gaaagtttga tgaacagaat gggaaattgt
     1681 ttagaaaatg gaacctctgt ctacggaaaa cgtcctcatg atccccttgc tgtctattta
     1741 actccggaaa actttccggt caaagctgac ggtataaccg atgacacgga tgctattcag
     1801 caggcgataa acgaggcgca aagaaggtcg ggctacggca ttgtctttat gcctgaggga
     1861 aattatcgca tcagcaagac aatatacata agcaagggta tacggttgat cggttacggc
     1921 aagaaacgac ccgtaattac cctggaagaa aactgcccgg attttcagga agcggatccc
     1981 gaagacaagg gccaggcaaa ctatatggtc tggtttacgg acagactgcc aaagcccggt
     2041 gagcccgtca gagatgccgg tgctggaacc ttttacagtg ccatatccaa tattgacttt
     2101 gtgatccaag acggtaatcc tacggcggta gcgctgcgca cccactatgc ccagcacagc
     2161 ttcatatccc atgttgacat ccatataggc caaggcaagg cgggaatgtt cgatgtcggt
     2221 aacgagatgg aaaatgtttg cttctttggc ggtgaatacg gcatctatac caccaagaca
     2281 tcgccgggtt ggccctttat gatggtggac acccattttg aggggcagag gagggccgct
     2341 atcaggaccc aagaggcggg acttaccatc gtcagaatga cagcccgcag tgtacccgtt
     2401 gtcattgaga ccgacccgaa cctctatgaa aagctgtata tggaggattc gcaattagaa
     2461 gacataagcg acgccgccct tataatcagc aacgaattca atacccatac ccaaatcaac
     2521 ctacggaacg tccattgtcg taacgtaaaa cggattgcta aatataggga gagcggaaag
     2581 atcatagagg gttcggaaaa catttacagc atcgaaaccc ttatccacgg taaccatatc
     2641 gatggcctga aaatccctgc agagatgaag gtcacatgcc atattaagga tttggactcc
     2701 ttcccaaatc cgcctaaaaa ggacataccc cagaccccgg gtattgagac atgggtaaat
     2761 ctgaaagaac taggggcggt aggcgacgga gaggccgacg ataccgaaat aatcaaggat
     2821 atcatctcaa agcatcgggt catctatgca ccacagggct tttatcgtgt aacggatacc
     2881 atagaattaa ggcctgatac cgtcctaatt ggtcttaatc ccattgccac aaggtttata
     2941 ctgaaggaca atacggaagg atttggcggt gtcggtgcgc caaaggctct gttggaggcc
     3001 ccaaaaggcg gcagaaatat agttatgggt ataagcctcg atacggcagg taagaatccc
     3061 cgggccgtgg gatgcaaatg gatggcgggg gcagattcat acatgaacga cgttaagttt
     3121 ttgggaggcc acggaggcat gaatccggaa ggcggctatg tgccttcata caacgcaacc
     3181 cgcacggcag atgcaaaccc agaatacaaa tgggataccc aatactggag cctatgggta
     3241 accgacggcg gaggcggtac atttaaggat atctggacag ctagtcccta tacagctgcg
     3301 gggctctatg tgtccgatac ctcaacggaa ggccgaatat atgctatgtc cgtagaacat
     3361 catgttcgca acgaggtaag gtttaaaaac gtatccaact ggaaggtgtt agccctacag
     3421 ctcgaagaag aggtagccga gggtccgtac tgtctgcccc tagagatcca agattgcagg
     3481 gatatggtct ttgcaaacct atatctgttt agggtaattt ggcatacaaa tccctatcca
     3541 tacgccataa agacatggaa ttgtaaagct gtcgagtttc tcaatataca caactacacc
     3601 cagataaaat atacctttga taataccttg tttgacgtta caaccaacac ccagatcagg
     3661 ccttgggaga tagccaggct ttatcttgat gggaagtcgt gtgtagatgg ggaagatgta
     3721 accgatactt gcgtctgctc agatgcaaca ggggacataa gggagctggc ggacgatttt
     3781 gagtttatag acggaatatg tgcggacagc aagggggaca tctacttctg tgataacaga
     3841 tggaagagga tatacaggtg gtctgccggg gataattctc tggcgctgct cacggagata
     3901 ccctataagc ctttggcctt gggcttcgat acggaggata atttgctcgt agtagtggag
     3961 tattccccgg ccaagggagc tacaatccat ggggagcttg agatatatgc aaaaccggag
     4021 gattcaagag gtacttccca cggatattgg tacagcgtaa attctacgac caaggttatt
     4081 gccatagatc caaataatcc cgacacgagc attgaatctt tagatcgggt ggacatagac
     4141 tcggtaaaag aggtatttaa agccctgcat ccggcaaaca ggtggaggga tggtcatgac
     4201 tttttagagg tctcagtcaa taaaccgtcc aagtgcttcg tggcacccga tggaattacc
     4261 attatcccgg tatgttatga cttgatgagg gccaccaact tgttggaggc ttatcccaat
     4321 aagccatttt atggggtaga tgaatataat aaaaagacgg cgatattcga tgtgaattcc
     4381 aaaggatatc tggaaaatcc cagaatcttc gccgaaaaag gggaaagcaa tgtagccgtt
     4441 gaccatgggg ataatgtcta tatacccgac ggacaggtat atatttacag cgcagacggc
     4501 aatttgctgg atgaaattga ggttcctacc aggccttccg gtgtgattat tgcgggtgaa
     4561 gacaaggaca ccctatttat tgcagcacgt tcttcgctat atgcggtttc cattaaacaa
     4621 taaatgtagt atactggata tgttccttga gcaatctaag cggtaagccc caaaaagtgg
     4681 atttgaactt ttatgctttc tggggcgaag ccgtctacat gttttacgat aaacacccta
     4741 tattgacatc tgggtgtctt aaaacataat ccataattga tatgttgtga aatacattgt
     4801 tttaatacaa aaataaatga tataattgat tttgtaacga tgagttttca cagcatatcc
     4861 aaataatagg gggtattata catggaaaaa atgaaagctg gttggattgg ttttataaga
     4921 cctgatgagg atttttgggg ggaacctgga aaaactctca gcccttggct accggggtat
     4981 ggaaggtggc gaggctctcc tcaaggggga cgtggaggat aacttaaaga ggtttcatga
     5041 tttaggcctt aaggttttga cggtttccgc ggatatggaa ggcctgcgtc agggcaatta
     5101 tgacggcatc atagaaaagg ccaaacaact gggaaccgac agagctaccg tttgggcatg
     5161 tgccattaat agctcgtttt ggaataagga gcccgtatat gatgaggtta tgagggatat
     5221 agaggccatg gaaaaggcag cagtgaaact ggacgaagag ggaatttgtc ttcgttatca
     5281 taaccaccat caggattttt tggtaagctt taagggaata acctgttttg atcatctcat
     5341 atacaatacc gaaaagctga gtattgaact ggatgtgggt tgggtgacca atggccttca
     5401 ggatccctcc gtagtcatgg agcggataaa ggatcggtta gatgctatcc atgttaagga
     5461 ttatctaaac ggcgaatatc gtgaaaagga aggcggctgg gctcccatat tcacgaccct
     5521 tgggaatggg gaattaaaac tgctggatgc tcttgaaact gcgcagggac ttggcattga
     5581 atgggcagtc gttgagcagg atcaaatgca caatctatcg cccatggaat ccttaactgc
     5641 gagttatctc aatatgaagg aaaccggtat agtcctctaa tcgaattcat ctgaagaagc
     5701 ggtgacatcg atgctttaag gaggtcccag aaggacatca tccaatattc tatacgtttt
     5761 acgataaaga ttctagattg agatacaggt atcgtaaaac attttcctta attcatatat
     5821 agctatatgc tttactacac ctataagtat tggataaata atataaatag ctactgagaa
     5881 ggggtgacgg ccgcaaatta gtcggctatc gccctatcat aaaagcttat ggagggatta
     5941 atgtgaaaaa tcgagacaag ctggttgtag gcgttctccc ggtaaagaga ggctttttga
     6001 gccttgaggt tgccatggag gagaagaaaa agctgtttaa tgccatcggt ggaatcaaag
     6061 atgattttgt aactctggtg gatgtagacg acgtgtgcga aggcggtgtc ctgtttaagc
     6121 tggacgaagt acaagctgcc gtggataaat ttaagaaagc cgacatagac gccctgttca
     6181 tctgtcattg cgactttggt gaagagcaag cattaataaa ggtcgctagg gaatttgatg
     6241 taccggttct tctatggggc aacagggaca aatttcccaa cactttcgag aagcggggta
     6301 gggataccca gtgcggtatg ttcgccagca ccaaggtgtt aagccgcaac aagataacct
     6361 ttagctatat ctataacgtg gatgttgaga cggacgaatt tatggacgga tacttaacct
     6421 tcctgaggac cgcaaacgta gttaaggcga tgaaacggct caagattgca cagatcggtc
     6481 taaggcccca gcaatttatg agcgttatcg ccagcgaagg gttgatggta gagaaatttg
     6541 ggatagaggt agttcccttt tctgttccgg caatcatccg taacgtagaa gaattagccg
     6601 ctgccaaggg ttcccaagtg caagagggaa tcgccagttt caaggagcga atggattgtt
     6661 cccagatgac ggaggaacag ctggccaaga tagctgccct taagataacc gtaaaggaca
     6721 tgatggagga ctcaggctgc agagcaggcg caatagaatg ctggagcctg ttttcaaggg
     6781 agataggcgt ggccccctgt atgatagtga gcgagttggc agatttgggg ttgcccctgt
     6841 cctgcgagat ggacctaaac ggcgccatca catccgtaat cgccgatgcc gtgaccttag
     6901 gagagagcgc ggtattcttt gcggatctga ccattcgcca ccctgaaaac gataacgctg
     6961 agcttttatg gcattgcggt cccttcccct attccctaaa agatccagac aaaccggcta
     7021 ggatgatcaa tgcccaggcc atatgggaac ttaaaaaggg tgatataacc attcttaggt
     7081 gcgacgatat agacggtgaa tacaaggtta ttgcctcgga aggcaaggcg gtagacggcc
     7141 ccgaaacgac gggttcttat gtgtggttcg aatccccatg ctggaagtcc ttagaagaga
     7201 agttcatctt cggcccatat atccatcacg ttgcaggggt atacggtaac tatagcagag
     7261 ctatagagga agccgcccgc tatctcccca tcaaatggga taaggtaggg acagggccta
     7321 ggagcttata aaaattggga tagtgtttta cgatacttga atactaatgt ataacgctta
     7381 tcgtaaaaca tacaggatag agatgggagt gacggaagat gcctattgaa aggacatctg
     7441 taatactaaa agacgctgag aaacacggat atggtgtagc cgcctttaat atattcaact
     7501 atgaaaccat cgcttgggcg atacaggctg cagaagaaga gaggataccc gttataatac
     7561 agttttatcc gggatttgac aggttcatac ccatggaagt ggtaacggcc gctactaagg
     7621 cgttggcggc taaggccagt atcccggtgg ggcttcactt agatcacagc aacaaatatg
     7681 aggatgccat aaggggtgcc gaatgcggtt tcacgtccat tatgatagac ggttctacgc
     7741 tggattttga tgaaaatgct gatttgacta aacgggttgt cgacacagcc cataagatgg
     7801 gcgtagaggt ggaggccgaa ctgggtcatg taggctcggg taataatata gacgacttta
     7861 caaacagcag ccactttacg gatccggaag atgctataag gtttatagag attaccaatg
     7921 cggacagctt ggccgtttcc gttggcaacg gtcatggaca ttacgtggct actccatcat
     7981 tggactttga tcggatacag gacatccacg attcaatatc cgtacccatc gttatgcacg
     8041 gcggctctga tatcccggat gaccagatac aaaggtccat cgcatgcggg atgagcaaat
     8101 ttaacattgc cacagagtac aatcgaagct tctacaacgc gatgaaaaaa tgtatggaag
     8161 aggatcaggg gcacggctat atgtttaggt gcctagccca ggcagaaaat gaagtcaagg
     8221 agtttttaaa ggccaagctg agggttctaa atcctaagtc atatcgcctg taacctgtaa
     8281 agtgggtatt gaccacagat ggaatcccta aataaggcgc agtgattggc aatcaattga
     8341 ctatcacgac aaaacatata tgcggttccc gatgctttga aaatagagtc ttggggaccg
     8401 ctaattttat cggggattgg ataatattcc ggggatgggc atattagata ttgacagcca
     8461 ttggggatag gtccggactt ctgacttcta atccccaact gctatctttt gggacctcaa
     8521 tcgatgaagt agtccgcttt tcaatagttc attgtctgga taatgaatgg gcataacgtg
     8581 ttaaattatt ttattaggag gttataagac tatggaaaaa atcagaggag ttaatctggg
     8641 aaattggttg gttcttgaaa aatggatgca tcctcccctg tttcatggag tggatgccga
     8701 ggatgaaacc gatctatgcc tgctgctttc cgacgaggat aaaagggccc gcctaaaagc
     8761 acatcgggac agctatatca cggagtatga ctttcgctac ataaagagta ttggtatgaa
     8821 tatagtgcgc ctgcctgtac cgcacttcgt ctttggggat cggccggaat tttgtgagcc
     8881 ctatgtccct tgcattgaat atgtggataa ggccttggaa tgggcgaaga tgacggggct
     8941 taagctttta attgatctgc ataccgcacc cgattcgcaa aatggctttg ataacggcgg
     9001 catatgcggg gtctgcaaat ggcataccaa ggaagagaat atagaacgca cccttgaagt
     9061 gttggagatg ctcgcaaaaa gatacaaaga taacgatggg ctgtatggaa tacaattgct
     9121 caacgagccc gttgccccct ttatgtggga atttgttaag gagcgctacc cggctaccga
     9181 tccggaaagg gcaaagggtt caggcggtgt gccgacggaa gtgttaaggg atttttatac
     9241 ccggggctac catagactgc gcaagcactt agatgaagat aaggtaatca tgtttcatga
     9301 cggttttcgc ctgaaggaat gggaaggttt tatgcagggg cccgaatata agaatatcat
     9361 gttcgatgct catatctacc ttgctatgac ggatcatgat atgccggaga ccatccgcct
     9421 ttcccattat cttaagaagc tggaggagta cgctgagggc ctaaagcata tgcgtcaatt
     9481 ctttccggtt attatagggg aatggtcact tatgcatagg ataaccggta tggaggactt
     9541 ttcaccggcg gagcttaggc attcctatag caccgttgcc aatgcccagc tctttacctg
     9601 ggagagcgca acggatggct ggatattctg gagctacaag ttgctttccg aaagtccggg
     9661 ctggagcctt cgagattgta tacaaaaggg atggttccct gaaagggtgt agcctatatc
     9721 attaatccat gacggattaa aatcattgtg tttaaaatag tggccttaac tttttatggt
     9781 ttctgttcat aaatttagtt gacctatcgt ctatctgcac aaaaattgat ggaaggtggc
     9841 gaatatgaga tttccacagg atattttcta tgggataaag gatgaggaga taatagaggt
     9901 gataaactct atccccaggg agacctttat accgaagagc ctaaaagggt atgcgtacac
     9961 agatacggca cttcctatag ggtccgacca gaccatatcc cagccgtcat tggtagccta
    10021 catgacccag cttttggggc ttacgggcaa ggagagggta ttggagattg ggacgggttc
    10081 cggatatcag accgctatac tggcgcgttt ggcccagaag gtatatacca ttgagataat
    10141 tcgggaactc catgataaat ccaaacagat cctagaggaa atgggcattt taaatgtctg
    10201 ctacaggctg ggaagcggct atgaggggtg ggaagaacat gctccctttg acgggatttt
    10261 ggtcacggca gcacccccga agattcccgc caagttgata gagcagctaa aggataacgg
    10321 aagaatggtg gtgccggtag gcgccagggg ccagacccaa atccttaagc tactggttaa
    10381 atcaggggat acaattatgg aagaggataa aacctatgta aggtttgtgc ccatggtcga
    10441 atagcggctc ttccccgtca aaggctgctg ttctcttgtt ctaaatttag gaggaaggtc
    10501 tttatttcaa ggcctccgcc atagcccacc aatgctccgt ttgatcctac aacacggtgg
    10561 cagggtataa ttatggatat agggtttctg ttgtttgcca ggcccacggc tctgtaggcc
    10621 ttatttttgc cgatggactc ggcgatgtcc ttgtagcttc gggtctggcc gtaggggatc
    10681 tttaaaaggc ttgtccatac cttgttcata aattctgtgc ccataggctc aagccttaag
    10741 tcaaattcct tcctccgacc acaaaaatat tcatccagtt gtctggcggc ttctgccaaa
    10801 atgggagttt cccctatgga ataacccctg ggaacggggt ttccttcaaa caagagatat
    10861 gtaatgttgt catcttgacc cccgatcatc agcctgccga ttggggtatc ataataaaag
    10921 acattaccca taataaaccc ttcctttcct tcaagccggt actaaaaatg tgcgatcaat
    10981 agccatcact tgcgttatca gccgataaaa atgaaaatat tcttcaccga tatacgacaa
    11041 aaccagccaa gagatttttt caatatagcc tatgtgttaa ttttatcaaa agaactatta
    11101 ttgtacaatg caaaaagatg tcgattatta ttatgactgg aaagggcccg aacccgccac
    11161 attgtttaaa ttgtatatcc acttcataac atatacaatt tggaccaaat tatggtacaa
    11221 taaccaaaag gaagggggat ttttcatgga cggatacaag atcattggat atttggccga
    11281 ttgggccgat tggacggccg aggatgtgga tgcccaaaag ctcacccaca taaactacgc
    11341 ctttgctacc atagatgacg gcagggtagt agcgaaggtt ctggatcgta gattggggaa
    11401 actccatatc cttaaggaga taaaagccca aaatccacat ctaaagaccc tcatatctgt
    11461 cgggggttgg ggggcggacg gtttttccga tgccgcactg actgaagaat ccagggagct
    11521 gtttgccgat actgccgtga agtttatgaa ggattacggt tttgacggtg ttgacatcga
    11581 ttgggaatac ccatgctcat ctgcagcggg aatagtctca agaccggagg ataaagaaaa
    11641 ctttaccctt atgctagagg agctccgcca gcagttggac cgggagggag aacaggatgg
    11701 caggcattat cttctcacca tagcagtcgg ggcgggacaa tattttatag acggcagcga
    11761 gatggggaag attcagaaat acttggattt tgtaaatatc atgacctacg attatgcaaa
    11821 cggtttagat gagcatacag gacatcatac ccatcttttt gaatccaagg tcaccccggg
    11881 gagcagcagc gataagtccg taaggctctt tatggggcag ggtatacccg catccaaatt
    11941 ggtattggga gtagcctttt acggtcgtgc ttggtccgga gtatccgggg aaaataacgg
    12001 cttaggcatg ggtggaatcc cgggtgcggg aggattggat acatcctata ccaatttgtc
    12061 atccaacctg gtcgataaaa acggatttga ccggtattgg gatgaggatg ccaaggcccc
    12121 ttacctctat aatggggata tatttatatc ctacgatgat gaagaatcca tcggacacaa
    12181 ggttgattat attaaggaga ccggacttgg cggcgtaatg ttttgggagt actcatcgga
    12241 taaaagcggt gcattactga aaaagctata cgaaggactt aggtcctgat ggtttaatga
    12301 tgatattggc aagggccagg gggattacag acaatctatg tataccctgc aaattaaccg
    12361 gggatgggga ttcgggtatc gtctgcatcg gttggagagc atgtatctgc aatgaaaaga
    12421 ttatcagtta tagtccacga gatcgatgat atgaatctca ggcttaatga aaatattaag
    12481 aatttcaaag tttagcacgc cacatgaaaa tccgacttac aattatcgac cttaaaaaga
    12541 tctcgtcata ggggtctttt tttatggtta ggtctggcta tcagcagttg aggttggctg
    12601 tctgtatttt accaatccca agacaaggat tgatgggcta acatacaagt cttgatttta
    12661 tcatggtgcg tattaaaatc cgggtattga cgcccaaaac gggtcatact ctttctggtt
    12721 taagaggggc tttgcataaa tcgcatcttt ggattgcgta catgctgaaa tgtcttgaca
    12781 tgtgtaataa atggatgtat tatataatgg caatgataac cgttatcaat taggatgaat
    12841 taatgggaat ccgcgtgttt tatgctaaat acttcatatt atgacctatt aggcataaaa
    12901 agcacctgta atcgcaaaag aatttcagca aatgatatcg gataataggg ggatgggaat
    12961 atggaactta aaactttgga ttctctcggc gttggggaaa aaggtataat aaccgctata
    13021 aatggcggta gaacggccac tataaggctg tgtgagatgg gatttaacag aggcagtgaa
    13081 gtctgcatag taaaaaatga tatagggccc attatcgtcg gccttgcagg cagtaagata
    13141 gctctcggat ggggattggc caacaagatc atggtgcagc agaaggagcc gggcttgcag
    13201 catgcagggg catggggtaa ggggggcatg agacttgcag acgatagcat tagtaggtaa
    13261 tcccaatagc ggcaagacca ctattttcaa cgcactgacg ggtactaacc agcatgtggg
    13321 gaattggcca ggggttacgg tggaaaaaaa ggaaggtatc gtaaagttta gcgataatga
    13381 atactccgtt at
//
LOCUS       DASRNX010000181         8670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_618997,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000181 DASRNX010000000
VERSION     DASRNX010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618997"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFD89_07280"
     CDS             complement(<1..285)
                     /locus_tag="VFD89_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HZJ58022.1"
                     /translation="MNKAIVIIIAALLVIAIGIGSFIIFLLIPSNKKDDEQPGYVYST
                     GDDFLTNLESEGHYIKADILIEVADKETLKILERNNHKIRDQIIEILGHID"
     gene            complement(343..1152)
                     /locus_tag="VFD89_07285"
     CDS             complement(343..1152)
                     /locus_tag="VFD89_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZJ58023.1"
                     /translation="MARKRTPNEDKKGAAEWLLTYGDLVTLLLTFFVMLFSFSTIDAQ
                     KWQSLVSSLAGQSGILEGGKVVNDTPNTGDNPSIDDFLELFPGGSDNLDDFNGNEDSE
                     YDDNDEFVRLYKAIEGFLEEKGIPAEVKISGTHTEILLRFKDSVLFDSGAANIKHDAK
                     EILKGVSQVLKEFHDHISRVRIDGHADIRPVDPRKHPTNWELSAERAVNVVRYFCEDQ
                     GFEASKLSAAAYGEFHPIEDDRTEEGLSRNRRVEIFIVRVVDSEEVTELDR"
     gene            complement(1155..1940)
                     /locus_tag="VFD89_07290"
     CDS             complement(1155..1940)
                     /locus_tag="VFD89_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZJ58024.1"
                     /translation="MDISTIAGIVAGIAFIIVGIIIDGSLQSFYDLPSIMIVLGGTLA
                     AIMVSYPLRRLAGFTKVANKLFFSSEERPREIISRIIELANIARKEGLLALEEAAYGT
                     DDPFLQKGILLIVDGTDPELVRNILETELDFVEERHREGQGIFETMASLSPAFGMIGT
                     LIGLINMLKQLQDPSSLGPSMSVALVTTFYGSMLANLFFTPMAIKLKYKSTREILLKE
                     IMLEGMLSIQAGENPRIIEEKLKAFLPPRDRTDEGGNPPEEDE"
     gene            complement(2028..2216)
                     /locus_tag="VFD89_07295"
     CDS             complement(2028..2216)
                     /locus_tag="VFD89_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HZJ58025.1"
                     /translation="MIKVTRLNDKEFVINTEIIEFIESTPDTVITTTTGKKVIVKEDV
                     DEVIDRVIAFKKRIFREK"
     gene            complement(2282..3616)
                     /locus_tag="VFD89_07300"
     CDS             complement(2282..3616)
                     /locus_tag="VFD89_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HZJ58026.1"
                     /translation="MLRSMFSGVSGLRSHQTKMDVIGNNIANVNTVGFKASRVTFQEV
                     YNQTLKAASAPTSATGRGGTNAHSIGLGVSVASIDVFHTQGGAQRTDKSTDLSLDGDG
                     FFVLSDGTRNYYTRAGNFDEDPFGYLVSANGLKVQGWAAKDGDGNIVTTGTTREINLA
                     NLSMPPHASSKIRFEGNLNSGLEDDGIVEYTTSIIDTLGEEHSIRLVFSRAERASDEG
                     GLEIDPNKWHLLIEPAGSDSKITTGTLYDTRDTGTFGAAEPIEFDGNGKLIYPLEGIV
                     IPSIEFSNGAVPIEQSGNTGGLRIEFDPEKMTQNDSESTFKIAESDGYEYGTLNNITI
                     DSMGRVIGIYSNGKARMDAVLAISRFNNPAGLLKLGDNLFEVSANSGDPIVGQAGIDG
                     RALINPGALEMSNVDLAREFTDMIITQRGFQANSRIITTSDEILQELVNLKR"
     gene            complement(3694..4125)
                     /locus_tag="VFD89_07305"
     CDS             complement(3694..4125)
                     /locus_tag="VFD89_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HZJ58027.1"
                     /translation="MKLGNENININRMVTVGKPIGNRYKSGINSSEAGPNATKVREQR
                     DFKDVLKTAIDNGNIGDVKFSKHAIRRMEDRRIELSAEQLRDIGDALNKVKNKGVKDS
                     LILMENTALIVNVPTGTVVTAVDNNSMRENVFTNIDGAIII"
     gene            complement(4139..4597)
                     /locus_tag="VFD89_07310"
     CDS             complement(4139..4597)
                     /locus_tag="VFD89_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HZJ58028.1"
                     /translation="MRVQSDNYIGGASEPSREPKTQLGKDEFLKILIIQLQNQDPLNP
                     MEDKDFIAQMAQFSTLEQIQNLGKDFQSFKAMNLVGRMIYGEIDIKNSSQIIPVLGRV
                     SSVTFSEGRMHLGVGDYTLGLDELMTVFSEEEAKTIELSNEADSTDNLGN"
     gene            complement(4610..5998)
                     /locus_tag="VFD89_07315"
     CDS             complement(4610..5998)
                     /locus_tag="VFD89_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HZJ58029.1"
                     /translation="MLQSINSCFIRLDDDIKVSDADHGESRPDRDKFRDILKTRSQVS
                     PKSHRRTKKEQIQTEEPNEGKKDITLPMDELLNVGAEVLYGQLIIPSIVPTPAGEEIL
                     YPKIPLISQYVGDVTDDVMDVIKETAFEGEADHDFNLAEEAVIEGIPSLVHRDEIISS
                     GKLHDNETLESVTEKVDIPLIKSETGDGPIENGLYDNDMEANEPTTSGPAIEELALEP
                     DILVRDKRFLENDAKGQMRDLEQVFVSSDDEDPGNSIRNEKAEIKFPAEAPGEYATRL
                     DNAHDGGDPRGKGLSDNDDDPIYNRDYNSDYLSNTISDAVLDFSKVLDAPNPEGKIEF
                     IEQLAEKAVVTLGNGKSALEIEVKPNHLGKLLLKVAVEDGNLTGRIYTDNRDVRDFLQ
                     RNLDDLWSTLKDQGLVFTSLDVNVGEQPSFDEFRHLKNVKSRARKIILANDIPLIKTG
                     EAVGSNRIDFFA"
     gene            complement(6102..6653)
                     /locus_tag="VFD89_07320"
     CDS             complement(6102..6653)
                     /locus_tag="VFD89_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58030.1"
                     /translation="MAKKRNRVPVMPIILLFLLVSGSLVIGFNIGGMRDVILAYIGKG
                     TARDTSGVDSDQIPDETDREFIKAEKTKLAALRTDLENYRTQLQDRENELQEKDAALL
                     DREREVQKLDEKLSSRFDDLIELTKIYERMDGEEAAAILSQIEDTHQVILIIKNLKKE
                     KSAEILGLMEPKKAADILGAMYE"
     gene            complement(6646..7110)
                     /locus_tag="VFD89_07325"
     CDS             complement(6646..7110)
                     /locus_tag="VFD89_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HZJ58031.1"
                     /translation="MNLKGFKFSLEAPLKVKGIHKTELEIKLAKAKNEMDDHKRTLDR
                     LSMESDGIQSRLGALLSTGIRAADLIEVNKYLGFLGDKIVDSQHRYHKASDKFTELRN
                     SLIGLMNEIDMLEKLRDQHMGEYLKGMQKKEERDLEERINYQTFMRGGKIYG"
     gene            complement(7107..8420)
                     /gene="fliI"
                     /locus_tag="VFD89_07330"
     CDS             complement(7107..8420)
                     /gene="fliI"
                     /locus_tag="VFD89_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HZJ58032.1"
                     /translation="MEPITFDRYREMLGDIKSISHTGKVTQVIGLTIESNGPSIELGQ
                     TCMLHNYKRTDSIKAEVVGFKEDRILLMPLGEPKGIGPGSLVESTHDTLKVAVSDDLK
                     GRVLDGLGNPMDDKGPITNQKLISIDNPPPSPLERKRITEPISLGIKAVDGLLTCGKG
                     QRLGIFSGSGVGKSTLLGMIARNTEADVNVIALIGERGREVQEFIHKDLGEDGLKRSV
                     LVVATSDQPPLIRVKAALVATTIAEYFRGKRLNTLLMMDSITRFAMAQREIGLAIGEP
                     PVARGYTPSVFAQLPKLLERSGNSSEGSITGLYTVLVDGDDFNEPISDAVRGILDGHI
                     ILSRDLANSNHYPAIEVLGSVSRVMPDIVSEDHLHIAGKIKNLLAVYRESRDLINIGA
                     YSRGSNPIIDEAIDKMAKINEFLQQSVGNRFDMGSTLERMSRIVE"
     gene            complement(8436..>8670)
                     /locus_tag="VFD89_07335"
     CDS             complement(8436..>8670)
                     /locus_tag="VFD89_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58033.1"
                     /translation="GKASLRVGEGDYPKILENKGLLLSKLDGIEEIDIVEDTGLEGGS
                     YVVDTGSGIIDGSVDAQMEVIESALLKSEDSIL"
BASE COUNT         2138 a         2232 c         1576 g         2724 t
ORIGIN      
        1 gtctatatga ccgagtattt cgataatttg atccctaatc ttgtgattgt tcctctccag
       61 gatcttcagt gtttccttgt ccgctacttc aattagtata tcggctttta tataatggcc
      121 ttcactttcc aggttggtta aaaagtcatc accggtactg tagacgtacc cgggttgctc
      181 gtcgtctttt ttattggaag gtatcaacaa aaatataata aaagacccaa tcccaatggc
      241 tataacgagc agtgccgcta taatgattac tattgcctta ttcatgataa gtgtcttgcc
      301 cccttatacg gttagtaata tcatccagta gcaatacagc tgttacctat ctaactcagt
      361 cacttcctcg gaatcaacta cccttactat gaaaatttct acccttctat ttctggataa
      421 accctcctcg gttcgatcat cctctatggg atgaaattca ccgtatgcag cggctgatag
      481 cttggacgct tcaaaacctt gatcctcgca aaaatatctg accacattga ctgcacgctc
      541 cgccgacagt tcccaatttg tcggatgttt tctgggatca acaggtctaa tatccgcatg
      601 tccgtctatc cttaccctgc ttatatggtc atggaattct tttaaaactt gactgacccc
      661 ttttaaaatt tccttggcgt catgctttat attagccgca ccggaatcaa aaagaacgga
      721 atccttgaat cttaatagta tttcggtatg ggtaccggat atttttacct ccgcgggtat
      781 tcccttttcc tccaagaagc cttcgatggc cttatagagc cgcacaaact catcgttgtc
      841 atcatattcg ctatcctcgt ttccattgaa atcatccaaa ttatcagaac cccccgggaa
      901 tagttcaaga aaatcgtcaa tgcttggatt gtcaccggta tttggagtat cgttgactac
      961 cttacccccc tccagaattc cactttgtcc cgccagggag gaaaccaggc tctgccattt
     1021 ctgggcgtct atggtggaga atgaaaatag catcacaaaa aaggtcaaca gaagggtaac
     1081 cagatcaccg taagtcaata accattcagc tgcacccttt ttatcctcat ttggcgttct
     1141 ttttctggcc aatcttattc atcctcttct ggtggatttc ctccctcgtc tgtcctatct
     1201 cttgggggga gaaaagcctt taatttttct tcaatgatac gggggttctc ccctgcctga
     1261 atggaaagca ttccttccag cataatctct ttcaggagaa tttccctggt gctcttgtac
     1321 ttgagcttta ttgccatagg agtaaaaaac aagttagcta acatcgatcc ataaaatgtc
     1381 gtaactaaag ccaccgacat actaggccct agggatgagg gatcctgcag ctgtttcaac
     1441 atgttaataa gcccgatcaa tgtgcctatc atgccgaaag caggcgacag agacgccatg
     1501 gtttcaaata ttccctgccc ctcccggtgt ctttcttcca caaaatccaa ctcggtctca
     1561 aggatatttc ttacaagctc ggggtctgtt ccgtcaacaa tgagcagtat ccccttttgt
     1621 aaaaatggat cgtcagtgcc ataagcggcc tcctctaggg ctagtagacc ttcttttctc
     1681 gctatatttg ccaattcaat aattcgggag attatctccc ggggtctctc ctcggatgaa
     1741 aagaagagct tgttggccac cttagtaaaa ccggccagcc gcctcagtgg ataagatacc
     1801 ataattgcgg caagggttcc ccctaaaacg atcataatgg aaggcaaatc ataaaaactc
     1861 tgtaatgaac cgtcaataat tattccaact ataataaatg caatcccggc aactatacca
     1921 gctattgtcg atatgtccat cgactacacc tcacttatga aatagataaa cgactctaac
     1981 tataaaactt atcacctata gacaaatttg gcttacctgc tgtttatcta tttctcccta
     2041 aaaattcttt ttttaaatgc tataacccta tcaattacct catcgacgtc ctcttttact
     2101 atgacctttt tacccgtggt tgtagtgatc accgtatcgg gtgtgctctc aataaactct
     2161 attatttcgg tgtttatcac aaattcttta tcgtttaacc ttgttacctt gatcatgtaa
     2221 acattcacca cccctagact aacttttacc agccggggct ataaccccgg ctggatatgt
     2281 cctaacgctt caggttcacc aattcctgta atatctcatc cgatgtggtg atgattcttg
     2341 agttagcttg aaatcccctt tgggtaataa tcatatccgt aaattctctg gccaggtcta
     2401 cgttggacat ttccagagct ccgggattta tcaatgctct accatctatc ccggcttgac
     2461 ccacaatggg atcaccggaa tttgccgaga cctcaaatag attgtctcct aattttaata
     2521 agcccgccgg attattgaat ctggatatag ccaataccgc atccatccgc gctttgccat
     2581 tggagtatat tccaattacc ctgcccatgg aatctatggt aatgttattt agagttccat
     2641 actcatatcc atcggattcg gctatcttaa acgtggattc gctatcattt tgagtcatct
     2701 tttcgggatc aaattctatc ctcaaacctc ccgtgttccc tgattgctct atgggcaccg
     2761 caccgttact aaattcgatg gatggaatta caatcccctc caacgggtag attagtttac
     2821 cgttaccgtc gaattcgatt ggttctgctg ctccaaatgt acctgtatcc ctagtatcat
     2881 ataacgtacc ggttgtaatt ttactatcgc tgcctgcggg ctcgatgaga agatgccact
     2941 tatttggatc aatttctaat ccgccctcat ctgatgccct ctccgccctt gagaatacaa
     3001 gccttatgct gtgctcttca cctaaggtgt ctataatact agttgtatac tctacaatcc
     3061 catcatcttc caacccgctg ttaagattcc cttcaaacct gattttagaa ctcgcatgtg
     3121 ggggcatgga caggttagct aggttgattt ccctggtcgt acccgttgtc actatattgc
     3181 cgtcaccgtc cttagcggcc caaccctgca cctttaatcc gtttgcagat actaggtatc
     3241 caaatggatc ctcgtcgaaa ttgccggcac gtgtataata gtttcttgtg ccgtccgaaa
     3301 ggacaaagaa gccgtcacca tctagggaca gatccgttga cttatcagtc ctttgtgcac
     3361 ccccttgggt atgaaacaca tctatagaag ctacagagac gcccagacct attgaatggg
     3421 cgttggtacc cccccgcccc gttgcactgg taggcgcaga agctgctttc aatgtctggt
     3481 tgtatacctc ctgaaaggta accctgcttg ccttaaaacc aacggtatta acattggcaa
     3541 tattattgcc tataacatcc attttagttt gatgagaacg gagtcccgaa acccccgaaa
     3601 acatggaacg caacataatt taacctcctt agttttgtgc gatcattgct ccttccttcg
     3661 ttccagagca tatagccccc taaaaaggtc cggctagata attatcgcac catcaatatt
     3721 ggtaaagaca ttttcacgca tactgttatt atccacagct gtcacaacag ttccagtagg
     3781 aacgttaaca atgagtgctg tgttctccat taaaatcaag gagtctttga cgcctttgtt
     3841 cttgaccttg ttcaaggcat cccctatgtc ccgaagctgc tctgcggaaa gctctatcct
     3901 gcgatcctcc attctacgga tcgcatgctt actgaactta acatctccta tgttaccgtt
     3961 atcaatggcg gtctttaata catccttaaa gtcccgctgc tccctgactt ttgttgcatt
     4021 tggtcccgcc tccgaagaat tgatgcctga cttatatcga ttgcctatgg gctttcctac
     4081 ggtcaccata cggtttatat tgatattttc atttcccagt ttcattttat ccaccatcct
     4141 aattgcccag gttatcagtt gaatctgcct cgttggacaa ctctattgtc ttggcctctt
     4201 cctcagaaaa aaccgtcatc aattcgtcta aacccagcgt ataatcgcct actcccagat
     4261 gcattctgcc ctcagaaaag gtcacgctgc ttacccttcc taacacaggg ataatttgag
     4321 atgagttttt tatatcgatt tctccataga tcatccttcc cacaaggttc atagccttaa
     4381 aggattgaaa gtccttgccc aagttttgaa tttgctctaa agtactaaat tgtgccatct
     4441 gggcaataaa atccttatcc tccataggat ttaggggatc ctgattctgc agttgaatta
     4501 ttaggatctt taggaattca tcctttccca gttgagtttt tggttcccta gaaggttcgc
     4561 ttgcaccccc tatgtagtta tccgattgta cacgcataat ttcacccccc tatgcaaaaa
     4621 aatcaatcct attcgatcct acggcctcac ctgtttttat caagggtata tcattggcaa
     4681 ggataatctt tcttgccctt gatttcacat ttttcaggtg cctaaactcg tcaaaactgg
     4741 gttgttcacc tacgttaaca tccaatgaag taaatacaag accctggtcc ttgagggttg
     4801 accacaaatc atccaagttg cgttgtaaaa aatcccgtac atctctattg tccgtataaa
     4861 tcctcccggt tagattgcca tcttcaacgg caaccttgag gagaagtttt cccagatgat
     4921 tgggttttac ctctatctcc aacgcagatt tcccattacc cagtgtgacc acagcctttt
     4981 ctgccaattg ctctatgaat tcaattttcc cctccggatt aggggcatcc aggacctttg
     5041 aaaaatccag tactgcatct gaaatggtat tgcttaagta gtccgaatta tagtcccggt
     5101 tatatatagg atcgtcgtca ttgtcagaaa gccccttgcc ccgggggtct cccccatcat
     5161 gggcattatc caacctggta gcatattccc ctggggcttc cgctggaaat ttaatctccg
     5221 ccttttcgtt cctaatagaa ttgccggggt cttcatcatc tgatgacacg aaaacctgtt
     5281 ccaggtcccg catctgcccc ttggcatcat tttctaaaaa ccgtttatcc cttacaagaa
     5341 tatccggttc aagagccagt tcctctatag caggcccaga ggtggtaggt tcgtttgctt
     5401 ccatgtcatt gtcgtataag ccgttttcta tggggccatc cccagtctcg gattttataa
     5461 gagggatatc caccttttct gttacggatt caagggtctc attgtcatgt agcttccccg
     5521 aggatatgat ttcgtctcta tgtaccaaac tcgggatacc ctctataacg gcttcctcag
     5581 ccaaattaaa atcatggtca gcttccccct caaaagccgt ttcctttata acatccataa
     5641 catcatctgt aacgtcgccc acatactggg aaattagggg tatcttagga tataagatct
     5701 cttcacctgc tggtgttggg acgattgacg gaattattaa ttggccgtat aaaacttcag
     5761 ccccaacatt taacagctca tccattggca gggttatatc ctttttccct tcattaggct
     5821 cttcggtttg aatctgttcc tttttagtgc ggcgatggga tttaggactg acttggcttc
     5881 tggtttttaa tatatcccgg aacttatcgc gatctgggcg gctctcccca tggtctgcat
     5941 ctgaaacctt aatgtcatcg tcgagtctta taaaacatga attaattgat tgtaacactt
     6001 ttacaccccc tttcattggt atttcttaat agatccatac ctggttttat aaaatgatca
     6061 atcaaagcag agtccaatag ttgccgcaac gttccccgcc attactcgta catagcgcct
     6121 agtatatcgg cggctttttt tggttccatc aatcccagta tctctgcaga cttttctttc
     6181 ttcaagtttt taataatcag gattacctga tgggtatctt cgatttggga tagaatagct
     6241 gctgcctctt ccccatccat cctctcgtag atctttgtga gttctatgag gtcgtcaaat
     6301 cgtgaggaaa gtttttcatc taatttttgg acctcccgct ccctgtcgag taatgcagca
     6361 tccttctctt gcagctcatt ttccctgtcc tgtaattggg tcctgtaatt ttccagatca
     6421 gttctaagtg ccgctaactt tgttttctcc gccttaatga attccctatc cgtttcatca
     6481 ggaatctgat ctgagtctac gccggaagtg tccctggcgg tacctttgcc tatataagct
     6541 aggatcacat ccctcatacc tccgatatta aagcctatca ccagggagcc ggataccaat
     6601 aaaaataaga ggattatggg cataactgga actcgattcc tttttttagc cataaatttt
     6661 tccaccccgc ataaaggttt ggtagttaat cctttcctct agatcccttt cttccttttt
     6721 ttgcatcccc ttgaggtatt cccccatatg ctgatccctg agcttttcca gcatatctat
     6781 ttcattcata agtcctatca gtgaatttcg taattcagta aacttgtcac tggccttatg
     6841 atatcggtgt tgactgtcca ctatcttgtc acctagaaaa cccaggtatt tattgacctc
     6901 tattaaatct gctgccctta taccagtcga tagcaaagcc ccgagtcttg attgaatccc
     6961 atcactctcc atacttaaac gatccagggt tcttttatgg tcatccattt catttttagc
     7021 cttggctagc ttaatttcca attctgtctt atgtatccct tttaccttga ggggggcttc
     7081 cagactaaat ttaaaaccct tcaaattcat tctactatcc tcgacatccg ctccaaagtg
     7141 gaacccatat caaacctgtt acccacggat tgctgaagaa attcgttgat cttagccatc
     7201 ttatctattg cctcgtcgat tatgggattg ctccccctgc tataggcacc tatatttatt
     7261 agatcccggg actccctata gacagctagg agatttttaa tctttccggc tatatggaga
     7321 tgatcctcgc ttactatgtc cggcataacc ctgctgacac tacctaaaac ctctatggcc
     7381 ggataatggt tgctgttagc taaatccctt gaaaggatta tatgcccatc taatatgccc
     7441 cttaccgcat cgcttatggg ttcgttaaag tcatcaccat ctaccaatac cgtgtagagt
     7501 ccggtgatgg agccttcgga tgaattaccc gatcgctcca gtagtttggg taattgggca
     7561 aataccgaag gtgtatatcc ccttgcaacg gggggttctc caattgcgag accaatttct
     7621 ctctgggcca tagcaaatct ggtaatggag tccatcatca aaagcgtatt tagcctttta
     7681 ccccgaaagt attctgcaat cgttgttgcc accaaagcgg ccttgaccct aatcaatggg
     7741 ggttggtctg atgtagcgac cactaaaact gagcgtttta atccgtcttc ccctaaatcc
     7801 ttatgtataa actcttggac ctccctgcca cgctccccta tcaatgcgat aacatttaca
     7861 tcagcttctg tatttcttgc aatcattcca aggagggtgc tcttccctac accgctaccg
     7921 gaaaatatgc ccaacctttg acccttgccg caggttagaa gaccatcgac tgcttttata
     7981 cctagggata taggctctgt aatccttttt cgctccaagg ggctaggcgg cggattatca
     8041 atggagatta gtttttggtt ggtgatgggt cctttgtcat ccatggggtt acctagtcca
     8101 tccaatactc tgccctttag atcatcgctg accgctacct tcagggtatc atgtgtggat
     8161 tccaccaagc tgccgggtcc tattcctttg ggctcaccca agggcatcaa taggatccta
     8221 tcctctttaa agcccactac ttccgcttta atagagtctg tccttttgta gttatgcaac
     8281 atacatgtct gccctaattc gattgatgga ccgtttgact ctatagtcaa cccaataacc
     8341 tgggtaacct ttccggtatg cgatatactc tttatgtccc ccaacatttc cctatatcta
     8401 tcaaaggtaa tgggttccaa ccatatcccc cctatttaca aaatgctgtc ctctgattta
     8461 aggagagcgg attcaataac ctccatctgt gcatccacgc taccgtcgat gatgccgctc
     8521 cctgtgtcaa ctacataact cccgccctct aaaccggtat cctctacgat atcaatttct
     8581 tctattccat ccagctttga aagcaagaga cctttgtttt ccaatatctt tgggtaatcc
     8641 ccttccccta ccctaagtga ggcctttcca
//
LOCUS       DASRNX010000182         5503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_566538,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000182 DASRNX010000000
VERSION     DASRNX010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566538"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..134)
                     /locus_tag="VFD89_07340"
     tRNA            complement(58..134)
                     /locus_tag="VFD89_07340"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(98..100),aa:Val,seq:gac)
     gene            complement(550..1128)
                     /gene="yfbR"
                     /locus_tag="VFD89_07345"
     CDS             complement(550..1128)
                     /gene="yfbR"
                     /locus_tag="VFD89_07345"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HZJ58034.1"
                     /translation="MSHFFAYLARMKFINRWGLMRNTLNENIQEHSLQVAMIAHLLVL
                     IHNRFYGGKVDVERVMTLAVFHEASEVITGDLATPIKYFNPEIKKAYKDIEEVANRRL
                     LEMLPKELQEDYAHILIEEKRDKNAYAFVKAADKISAYLKCLEELKAGNREFEKAKNS
                     IEEQIWEMDSPEIAYFMENFAPSFSLTLDELN"
     regulatory      1404..1496
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            1577..2101
                     /locus_tag="VFD89_07350"
     CDS             1577..2101
                     /locus_tag="VFD89_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HZJ58035.1"
                     /translation="MDNRIRNVSLAGLFVALSIVLARFFAGDILLAGTSVLRISFGPV
                     PIYLSGMLLGPVYGAMTGILADSLGYLVKPLGAYFPGFAINGALSGIIPALLAKYYRE
                     KESWARLFLIVVPTEIITSIFLTPLWLSMMMDKAFIAFIPSNLISRIFLVPAYVALIK
                     TVLKLSKQAIPSLK"
     gene            complement(2181..3533)
                     /locus_tag="VFD89_07355"
     CDS             complement(2181..3533)
                     /locus_tag="VFD89_07355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HZJ58036.1"
                     /translation="MRKSRIHIALMLCLLLIYALLAGCSLIKRFSAGPNIRNIGQNHV
                     TEDDNNKDNNNRGDNNKDDNNKMGDDGRSQVISEGSPNGLEDPSNGAEETIADPKPKG
                     LTKLKISVVGDIMVHNHQLKAAREPDGSYDFTGVFEDISPYLKEADIAMGNLETTIST
                     DEIGYTAYPRFRSPKSLVTALKGAGFDILTTANNHSLDGVEFGVEHTLDVLDEHGIPH
                     TGTARSPEERDRILIVEENDIRVAILAYTYGTNGMEGSVNKDKLTYMVNYLKDTSKIE
                     DDIQRAKDEGAEFIVVCAHWGNEYVRTPNEAQKQMADKLFDMGADVIFGSHAHVIQPM
                     ERRDITTPQGEEKETFVIYSLGNIVSNQRDRYRDSGLILNLEIIKDYDNETIGLGEID
                     YIPTWVYRFTKNNRLHYRILPVRDFLEADFGAADNKRIKEVWKETTEHLDTEGIRVRD
                     "
     gene            3918..5357
                     /locus_tag="VFD89_07360"
     CDS             3918..5357
                     /locus_tag="VFD89_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HZJ58037.1"
                     /translation="MKNLKYLFCTVLVFLTLLSLIVPTIILANDPPTLYTMYINGQEI
                     GIVRFPARALAIYDEIETGLREKYTEEVFIGFDVYFKEIAAGSRQVTNESILTKSIEE
                     TVDVKVNAYAVSINGAKMFFVNTLQEAEKVVQEIKAPYIKRIEDKGESKLEEVTIKED
                     ISLDEELISQSQVITPEEAIEMVLNGTEGFREYKVEKGDSLWDIANRNNVKVSDLQLA
                     NPDIDNDIIQPGDILKIGRQEKLLTVLTKEQAEYTKEIPFETEVKEDKDLEKGKSKVL
                     QEGEKGEKKIVALITREDGQEIARDIISEEVTKEPIKHIEAKGTKVKPKPQARPTPSR
                     SSNKSSSKSSTKKVSAPSSRGSGSGSDIANYARKFVGYPYKWGTAGPNSFDCSGFTYY
                     VYKQFGIDLKTTGSVAQRKVGSKVSKSDLKPGDIVCFTSSSASGHVGIYIGDGKMVHA
                     SGKKTGVKISDINSGSYPKRYVTSRRVVK"
BASE COUNT         1515 a         1262 c         1151 g         1575 t
ORIGIN      
        1 ttattaatat ggcaatatag cgatatagca ccggaatatt ccgatgctat atcgctctgg
       61 tggtggctac tggagtcgaa ccagtgaccc ccacgatgtc aacgtggtac tctaaccagc
      121 tgagctaagc caccaaataa tgatgtttca aaaaaagtgt ccaattgctt aaggcataat
      181 acctataatt cgttttatta aacccaacct cattgggcaa acacttgata acccataagc
      241 aaaattatta tagcatgctt ttatctccca gtcaaacatc gcaggaattt ttttacccct
      301 aggcgcaagt tataaattca agaaggacct tcataaaaag aaggtcctcc gtaaaagggc
      361 tgttacgatc ccaataacaa tagatattca ccttgttgcg actacacgct gccatctacc
      421 gaatcccctg caggttttac aggtcgtaat aaaacccttg tgagaatatt gttgtcgaaa
      481 aacagaaaaa taaacatggt aatatttttt ggtccctatt atctgatatt tccccatacg
      541 atgtcactgt taattcaatt catccagggt gaggctaaag ctgggcgcaa agttttccat
      601 gaagtaggct atttccggcg aatccatttc ccaaatctgc tcttcgatgg aattctttgc
      661 tttttcaaac tccctgtttc cggcctttag ttcttcaaga catttgagat aggcgctgat
      721 cttgtctgcg gctttaacaa atgcataggc gtttttgtct cgcttttctt caatcagaat
      781 gtgggcataa tcctcctgca attctttggg aagcatctcc aagagtcttc tgttggcaac
      841 ctcctctata tccttgtatg ccttctttat ctccgggtta aaatatttta tcggcgttgc
      901 caaatccccg gttatgactt ccgaagcctc atgaaatacc gccagggtca tcaccctttc
      961 gacatccacc ttcccgccat aaaatctgtt atggataagg accaacagat gggctatcat
     1021 agctacctgc agactatgct cctggatatt ttcatttaaa gtgttgcgca tcagtcccca
     1081 cctgtttatg aacttcatcc tggctaagta cgcaaaaaaa tggctcatag atactcctcc
     1141 atttccatag acatgctact ttccttttcc cttctacaat aattcatatc ttattcgtct
     1201 gcaacttttt ttatttacat atctttttga tttgaagaat tatatggaat attattctta
     1261 aaattttaga ttatgaatcc ttttttatgc ccgtcttgca acttctatca cattttgtta
     1321 taatcagtaa tactatgaaa cgtatgcaat ataacccata tgggatccat tttaaaaatg
     1381 aatattgcgt ttcatacaga atgggcagag taagtattgt gcgttaagtg ttaagggatg
     1441 ggaagtcgcc cttaaacgaa ggatcttatc cgcggtacaa tgctgcgtcc gctgccacat
     1501 acaagagact tttaatgagc acctactctc tttgttgtgg gcttctttgc cgttcaaaga
     1561 gaaaggaggt gtttttttgg ataacagaat aagaaacgtt tccttagcgg ggctttttgt
     1621 tgcgctgagc atagtcctcg ctcggttttt tgcgggggat atcctcctgg ccggtacatc
     1681 cgtccttagg ataagctttg ggccggtacc catctatctg agcggaatgc tcttagggcc
     1741 cgtctacggg gccatgaccg gcatattggc ggattcattg ggctacttgg tcaagcccct
     1801 gggtgcgtat tttccgggct ttgccataaa cggtgcactg tctggaataa tccctgcgct
     1861 cctcgccaaa tattacaggg aaaaggaatc ctgggccaga ctatttttga tcgttgtacc
     1921 gacagagatt ataacatcga tttttcttac cccgctatgg ctcagcatga tgatggataa
     1981 ggcctttatt gcttttattc caagtaatct gatatccagg atctttttgg tgccggcata
     2041 cgtggcgctc atcaaaacgg tcctgaagct ttcaaaacag gcaattccct cactaaagta
     2101 acccatatac gaatcgatac aaaactaaaa aaggataggg ctacccaagc ggttgggtcc
     2161 tatccttttt aattttacaa tcaatccctg acccttatcc cctctgtatc caaatgctcc
     2221 gtggtctcct tccacacctc ttttatcctc ttgttatccg ccgcgccaaa gtccgcctct
     2281 aaaaaatccc tgacgggcaa aattcgatag tggagcctgt tgttctttgt aaacctatat
     2341 acccatgtgg gaatgtagtc gatttcccct aggcctatgg tctcgttgtc gtaatctttg
     2401 ataatctcaa ggtttaaaat cagcccgctg tccctgtatc ggtcccgctg gtttgaaact
     2461 atgtttccca aagagtatat cacaaaggtt tccttctctt ccccttgggg agttgttata
     2521 tcccttcttt ccatgggctg aattacatgg gcatggcttc cgaagattac atctgccccc
     2581 atgtcgaaga gcttatccgc catctgcttt tgcgcctcgt tgggggttct aacgtattca
     2641 tttccccaat gagcacatac gacgataaat tctgccccct catcctttgc acgttggata
     2701 tcatcttcta ttttggaggt gtccttcagg tagttgacca tataggttag tttatcctta
     2761 ttgaccgacc cctccatgcc attggtcccg tatgtataag ccaagatggc gacccttata
     2821 tcattttctt cgacaatcaa tatccggtcc cgctcctcag gcgacctggc tgttcccgta
     2881 tggggtatgc catgctcgtc cagcacgtcc aaggtatgct ctaccccaaa ttctacaccg
     2941 tctaagctgt ggttgttggc ggtggtcagg atatcaaacc ccgctccttt aagcgcagta
     3001 acgagggatt tagggctcct gaacctggga tatgcggtat aaccgatctc gtcggtgctt
     3061 atggtggtct ccaagttacc catggcgata tcggcctctt taaggtatgg gcttatatcc
     3121 tcgaatactc ccgtaaaatc ataggatccg tcgggttccc tggcagcctt tagttggtgg
     3181 ttatgtacca tgatatcgcc aactacgctg atctttagct tagttaggcc ctttggtttt
     3241 ggatcggcta tggtttcttc cgctccgttt gaggggtcct ccaatccgtt cggagatcct
     3301 tcagaaataa cttgggaccg cccatcgtct cccattttat tgttgtcatc tttgttgttg
     3361 tcacctctat tgttgttatc tttattgttg tcatcttcag ttacatggtt ttggcctatg
     3421 tttctgatgt tgggcccggc agaaaacctt tttataagtg agcagccggc caatagcgcg
     3481 tagatcagta acagacacag cattaaagca atatgtatcc tagactttct cattctcttt
     3541 ctcccgtaat ctattaattc accggtgtta ttttaattta tgtcaactcg gcttaaagtc
     3601 tcggcaatac cgatccttta atagtgcaga ccctccgtga gcacgggccc ttacgcgaat
     3661 ccttaaatgg ggcccgtagc aattcatgca tattacacag aaatagtacc agaagtaaat
     3721 ggtaatttca atatgcaatg agtcgtattg accaaaactt tcctaaatag atttcataaa
     3781 aaaaatattt ttgtaaataa tacaaaacta ggaaggatat tttacattta tgtagaatat
     3841 agaattatca atgtaacact tttgttaaac tttgagataa aattgtaata gactggatat
     3901 cttaactgga ggttatcatg aagaatttaa agtatctgtt ttgtaccgtg ctggtatttc
     3961 taacccttct ctcccttatc gttcccacga taatcttggc caatgatccg cctaccttat
     4021 acaccatgta cataaacggt caggagatag gaatcgtcag attccccgcc agggcgctgg
     4081 caatctacga tgagatagag acgggtctta gggagaagta taccgaagaa gtttttatcg
     4141 gtttcgacgt ctattttaag gagatagcgg caggttccag gcaggtcacc aacgagagca
     4201 ttctgacgaa atccatagaa gaaaccgtcg atgtgaaggt taacgcctat gctgtaagta
     4261 tcaacggggc aaagatgttt ttcgtaaata cactccaaga agccgaaaag gtcgttcagg
     4321 aaatcaaggc cccctacata aagaggatag aagataaagg ggaaagcaag ctggaagagg
     4381 tcaccatcaa ggaggatatc agcctcgacg aggaattgat atcccaaagc caggtcatca
     4441 ccccggaaga agccatagag atggtgctta acggtaccga aggctttagg gaatacaagg
     4501 tagaaaaagg tgactccctt tgggacatag ccaatcgtaa taacgtaaag gtatccgacc
     4561 tccaactggc aaacccggat attgacaacg atataatcca acccggggat attctgaaga
     4621 taggccgaca agaaaaactg cttacagtcc ttacaaagga gcaagcggaa tataccaagg
     4681 agattccctt tgagacggag gtaaaggaag ataaggatct ggaaaagggt aagtccaagg
     4741 tcctccaaga gggcgaaaaa ggggagaaga agatcgtcgc cttgatcacc agagaggatg
     4801 gacaggagat cgctagggat atcatcagcg aagaggtgac taaggagccg ataaaacaca
     4861 ttgaggccaa gggcaccaaa gtaaaaccca agccacaagc aaggcctaca ccctccaggt
     4921 catccaataa atcatcgtct aagtcttcca caaagaaagt ctccgctccc agtagcagag
     4981 gcagtggcag cggcagcgat atagccaatt atgcccgtaa atttgtaggc tatccctaca
     5041 aatggggaac cgcaggccct aactccttcg attgttccgg atttacatat tacgtgtaca
     5101 agcagtttgg aatcgatctg aagacaactg gctccgtcgc ccagcgtaag gttgggagta
     5161 aggtttccaa atccgacctt aagcccggag atatcgtctg ttttacaagc tccagtgcca
     5221 gcggccatgt tggaatctat atcggcgacg gcaaaatggt acatgcatcg ggcaagaaga
     5281 ccggggtcaa aatcagcgat ataaactcgg ggagctaccc aaaacgctat gtaacatcca
     5341 gaagggtagt aaagtaagct aaaaattaat gaagtcatcc cctttacaac agcatacttc
     5401 tcagataatg aaccggcacg atgcgtcaat ttgttaagac cattattaag gccataggat
     5461 aattaataga aatgattaat atacaaagac accaaagagc aac
//
LOCUS       DASRNX010000183         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_88194,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000183 DASRNX010000000
VERSION     DASRNX010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88194"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..995
                     /locus_tag="VFD89_07365"
     CDS             141..995
                     /locus_tag="VFD89_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019153503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZJ58038.1"
                     /translation="MKYLVKRNLLIFFRDRGSVFFSLLAVFIIIGLYVLFLGDMLINN
                     MQEMPGARFLMDSWIMAGLIAVTPITTSLGAMSSIIVDKKYGMFKDFAASPIKRSSIA
                     LGYIASGFLISLILTLITYVLAEVYIIAFGGNLLPFTAMIKVLGMILFTTASSSIMMF
                     YLVSLFKSINAFSAASTVVGTLIGFLTGIYIPVGSLPPAVQTAVKLFPPSHGAVILRR
                     IMMGQAEKITFRGAPAAALDAFRLEMGASFRVGDIRLNSYASLLYMALFMAGFTVLAL
                     IRIKRKGD"
     gene            1259..2491
                     /locus_tag="VFD89_07370"
     CDS             1259..2491
                     /locus_tag="VFD89_07370"
                     /inference="COORDINATES: protein motif:HMM:NF022889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HZJ58039.1"
                     /translation="MPKDLLQIILKSAGDSFIQVTVFVGAVLLLFGYINFRQQGAFVE
                     RIEKSKRYQPLIGAFLGMTPGCGGAIFVMPLYVKGTVSFGTVVATLIATAGDSAFVTL
                     TKAPKDFVIVTSISFVVGAITGYIIDHYNIGDWVRKRSRRLSKIDVEREHKHAEAMMD
                     ELYCDNPNACRSNTLRHIGHEEGDEVDLALHHSKPLDVTSLGYRVTHGAYVVFWIILA
                     AGFILGILDLAMVDINALPGLPNLGLIIGLLGTIATVLYMIFSKKFIKATSHEEVEHK
                     LFSLKETFIHNAEETAFVGTWVFAAYLAYELGVFAIGGEHVIEAALTSTGLTAVLIGA
                     AVGLIPGCGPQIIFVSLYLKGMFPFAALLANAISQDGDALFALIALDPKSAFWSTVYN
                     TIPALIVGILAYYIQLAL"
BASE COUNT          731 a          542 c          643 g          824 t
ORIGIN      
        1 accataaccc tgaaactgaa gtccaccatg gaggcattgc ctatattgga aagggtgggg
       61 gacagtatcc ggggttttca ggtaattgaa ggggacatgg acgatgcctt tatcaatatt
      121 acagggaggg agatgaggga atgaaatatc ttgttaagcg gaacctgctg atttttttcc
      181 gtgaccgggg cagtgtcttc ttctcactct tggcggtatt tataatcata gggctttatg
      241 tcttgttttt gggtgatatg ctgataaata atatgcagga aatgccgggt gccagatttc
      301 ttatggacag ctggataatg gcgggtttga tagccgtaac gccaattact acctccctgg
      361 gtgccatgtc gtctatcatt gtggataaga aatatgggat gtttaaggac tttgctgctt
      421 cgcccataaa aaggagctct attgcactgg gctacatagc aagcgggttt ttgataagcc
      481 ttatactaac ccttataacc tatgtgcttg cggaagttta tataatagcc ttcggcggaa
      541 atctgctgcc ttttactgcg atgataaagg tgctgggaat gatcctgttt accacggcat
      601 cctccagcat aatgatgttc tatctggtat ccctctttaa gagcatcaat gccttttcag
      661 ctgccagtac cgtcgtgggt accctgatag gatttctcac gggtatctat atcccggtgg
      721 gatctttgcc gcccgctgtc caaacagctg ttaaactttt tccaccttcc catggggcag
      781 ttatactgag aaggatcatg atgggccagg cagaaaaaat cacctttagg ggtgcacccg
      841 cagcggccct tgatgccttt cgcctagaaa tgggcgcatc ttttagagta ggggatataa
      901 ggctaaattc ctatgccagc cttttgtata tggcattgtt tatggcgggc ttcacagtgc
      961 ttgcccttat aagaatcaaa agaaaagggg attaggttaa tatgctttaa aatatcggaa
     1021 aattgtttga tgtatcccca tggggttata aatactctgg tattggatta accagttgtg
     1081 ctaattcttt atactaccat agataagcta ctctaaagat aaaatttatc gtcatttctt
     1141 gcatagaccc attgcatttg tcatattgat aaatttagtc tttgtattgt ttatctataa
     1201 ttaatttctt tcgatcattt aaaaccaaat tatcatggaa agtggggaag gggataaaat
     1261 gcccaaggat ttgttacaaa ttatattaaa gagcgccggg gattccttta tacaggtaac
     1321 ggtcttcgtc ggtgcagtcc tattattatt tggttatata aacttcagac agcagggggc
     1381 cttcgtggaa agaatagaaa aatccaagag atatcagccc cttataggcg catttttagg
     1441 aatgaccccg ggatgtggcg gggcaatatt cgtaatgccc ctctatgtaa agggtaccgt
     1501 cagctttgga actgtagttg ctaccttgat agcaaccgcc ggagactcgg cttttgtgac
     1561 cttaaccaag gctccaaagg attttgtcat agtaacctct atctcctttg tcgtaggggc
     1621 cattactggg tatataatag accactacaa tataggggat tgggtccgaa aaaggtcccg
     1681 aaggctgtcc aaaatagatg tagaaaggga gcacaagcat gcggaagcca tgatggatga
     1741 actctactgt gataatccca atgcctgtag atccaatacc ctgaggcata taggacatga
     1801 ggaaggcgat gaagtggact tagcacttca tcacagcaag cccttagatg tcacaagtct
     1861 tggatatagg gtcacccatg gcgcctatgt tgtattttgg atcatattag ctgcgggatt
     1921 catcttaggt atcttagatc tagccatggt ggacataaat gcattgccag gcctacccaa
     1981 tctaggcctt ataattgggc tgttaggaac cattgccacg gtattatata tgatattttc
     2041 taagaagttt attaaggcta ccagccatga agaggtggag cataagctat tttctctaaa
     2101 ggaaaccttc atacacaatg cagaggaaac agcattcgtg gggacctggg tctttgccgc
     2161 ctacttagct tacgagttgg gggttttcgc catcggcgga gaacatgtga ttgaagcagc
     2221 cctgacatcc acaggtttga cggcagtctt aattggggcg gcagtaggat taatacctgg
     2281 gtgcggtccc cagattatat tcgtatccct ctatttaaag ggcatgttcc cctttgcagc
     2341 ccttttggcc aatgctattt cccaggatgg agacgcatta tttgccctta tcgctctgga
     2401 tccaaagtca gcattctggt ccacggtgta taatactatc ccggctttga tagtgggtat
     2461 cctggcgtat tatatccaac ttgcccttta gagcgattgc gtatgaaatt taactatagg
     2521 tatttagcat ggggttcttc ggccatgaaa ttattacatc ggaagcaatt gttctggcaa
     2581 ttacaacaca atattgcttt atctcaaaat taagtcaaat tgggattgct tgaatgattt
     2641 actttaatat agtagactcg aatctggatt attgatattt tgcttaagga ggccactatg
     2701 agtcaatata cctttgggat aagacccagt ttagctactg
//
LOCUS       DASRNX010000184        11813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_251149,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000184 DASRNX010000000
VERSION     DASRNX010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251149"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VFD89_07375"
     CDS             <1..674
                     /locus_tag="VFD89_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZJ58040.1"
                     /translation="FKTNINILIMYLIPILLAYIYDNFIPGMPEGFALSFGLLFLVVM
                     VGMYVPSMMIAEEKEKKTLEVLLLSPASSVEIFIGKGLLTFISILISFIILVAISGRV
                     GIDHIGVILIGGLLTAVFCIQVGIIVGLLSKNQMATGVVGTPVYMALLLIPMLGSMGS
                     PVLKKIAKVFPTYYYNDMLYKALGGFEGSQTLLDMGPELGILLGSILIAFVLLLVVYR
                     KKGLN"
     gene            756..1442
                     /gene="sfsA"
                     /locus_tag="VFD89_07380"
     CDS             756..1442
                     /gene="sfsA"
                     /locus_tag="VFD89_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HZJ58041.1"
                     /translation="MKYNGIRRAKFIDRPNRFIANVELDGKREMVHVKNTGRCREIFV
                     EGTTVILEEGKNPNRKTRYSIIGGYKGDLLINTDSQVPNAVVYEAITQNKIEEVQNVT
                     KVKREVTFANSRFDIYFEDMSRKGFIEVKGVTLEYDGVCMFPDAPTQRGARHVHELIE
                     AKRQGYHSYIIFLVQMEGMKCFTPNYRMDPEFGEALKTAHSRGVKILVYDSVVGEDEI
                     AIGNSLGFHL"
     gene            1482..3407
                     /locus_tag="VFD89_07385"
     CDS             1482..3407
                     /locus_tag="VFD89_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58042.1"
                     /translation="MMRNKRSVIFKSLISLTLIMALALPGYGNKLSIAEGKITSSERL
                     VGDNVGLIEGNYYVLSQSGFMLNYEIQNGTENNITQIILEETLFDESGRVVPGEGAPD
                     TEFIIEAGQKKTFQGKKFKAQEKPTAYTLEYSIQYILEDGEGPGLISSGKRQITVLST
                     GLSVVYKASTGGPIPQGSEVTYTFEIKSSANININNILVQDSVLGDIGVIPILAPDET
                     ATLSMTLKLNETTKSYPIIIFEGPMDGQGNIKREFKNAAVEVVVEEKQVENPLVIIGK
                     ASKSKIAPNEEVDFSLIVENKGNRTLTKVSLTDWTGKEILTKDNLAPGKEGVIIHAWR
                     VEPGKEYTFKATAVEEGTDRKVQASYTAKFSAIEAEMEIANRVTPQEVAAGDTVTIEY
                     ILKNTGKATMVDVRVQEPEFGEVGSFDVLKPGQTETFAIERVIEKDTGSHPMVYAKDK
                     DSGYEYEFQGDLIEIAVNIVETRPLLTIKLATEPESLTEAGAVDLICTVINEGDVKID
                     NIDLVLNERELGIGSILTLESGDEETLTLPGLDIGEDTSFTVTARGVTYLGEEVEFVS
                     APYEITIGGDDIPEEPIKNPKISFLKKLLGIIAILGVATVGGMVYLLRDLRRGGKKKT
                     VGGGSKQRARKRKTTRK"
     gene            complement(3448..4008)
                     /locus_tag="VFD89_07390"
     CDS             complement(3448..4008)
                     /locus_tag="VFD89_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58043.1"
                     /translation="MLRSIIRPVITLWEKFFSWAENINPIENSPYGLLRMSVHPYKGR
                     PTSLDDGTEVSPGDYVLEIHISNLTLAQGVVAGVEVASDIQLLRLFREEIAHLACLAQ
                     RGKLDPKVKAVWGVTMMGPAMKRLGFALEPMDEDMGSKIIIAWMTFLKWVFSPGDTKT
                     ASRKKRRRKGYQYWMSLDQLVNKYTH"
     gene            complement(4025..4708)
                     /locus_tag="VFD89_07395"
     CDS             complement(4025..4708)
                     /locus_tag="VFD89_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZJ58044.1"
                     /translation="MWWLFPAVLWIIFLIYCLLPNYWARNCSSKVLRGGSSNKRKIYL
                     TFDDGPNPDYTPRLLDILGEHQIQATFFLMGRQAELYPDIVKRIAEEGHTIGCHSYSH
                     RHAWLMSPVLTFKDMSRAYEAISLASGIFPKWYRPPWGGFNLFSIAAAGRLGLGIAYW
                     SIEAQDWDINTAAEHIHETVVDKAVPGSVIVLHDNQGDPGAPENTLMALPMIIRSLRK
                     DGYSFASLE"
     gene            complement(4767..5966)
                     /locus_tag="VFD89_07400"
     CDS             complement(4767..5966)
                     /locus_tag="VFD89_07400"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZJ58045.1"
                     /translation="MSQEDFKKCDVLILSADFGTGHHQVSVALKGVFKERRPHWEVGI
                     YNYFQYIGPLFNKSLKFCYSQMIRHFSWGYDWFYRITRETDSRSKWQQMLNSMGRKRI
                     YELILEHSPKVIICTFPTPAGVVSQLKREGKIDIPLAVVITDVTAHGQWIHPMADAYI
                     VAADVVSQLLIEKGIPAKNIHVTGIPIRSQFEAPVPDPGIWIRHNLDQSLFTLLIMGG
                     GAGLMPGIERICERLGDMGLPMQIVALAGSNRALAKNLEEVARRSPVPVRVLGYTENI
                     APVMRGSHLLLSKAGGITVFEALAVKLPILLYKPLPGHEMGNVNFLLDNGAALIAQNE
                     NQVVELIKNAVEDPSVLENISHAMAHLSKPSSARHAVSAIVGLVSHDHYIEPGDEISL
                     EKKDRYA"
     gene            6491..7072
                     /locus_tag="VFD89_07405"
     CDS             6491..7072
                     /locus_tag="VFD89_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZJ58046.1"
                     /translation="MNIESQLIERSVSGDTDAFGELVLLYDKRIYNYCFRMTNNADDA
                     EDLAQEVFIKVYRNLGSFKGNCKFSTWIYRIAHNTCIDNHRKKRFRLLSLSLQGDEKG
                     EMDIPDKGPSPEEQIVSREQYGLMKECIARLKPEYKSVIILRDIQNYTYQEISDILEI
                     PLGTVKSNISRARALLRDTLKSRLAAYGERRVN"
     gene            7072..7872
                     /locus_tag="VFD89_07410"
     CDS             7072..7872
                     /locus_tag="VFD89_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZJ58047.1"
                     /translation="MDCRDFDYYISLYIDKMLDEGDKEKLVNHLKECQRCSEGFRKTQ
                     QIVSMINSLESEPLPHDFNDKLLGKIGHGKRIASPHWLRWTGAVAGILVIFFSIKIAL
                     DVGVTDKASSTATLTEGMKGEDIADGSELMQMESPGEARDDLEADSAQYDGVEDSVER
                     EGIGDGYDEEMVSKEDSQLNDLPRIQSNEVEIYVEDICITPETLKFIAIDNELELIES
                     GEGFIVIGIHSQKERDILHRELSKLGEVREVGEEMDTDQVKLIIRAAE"
     gene            7982..8794
                     /locus_tag="VFD89_07415"
     CDS             7982..8794
                     /locus_tag="VFD89_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58048.1"
                     /translation="MQDNTAKSFLDPILISLMKKEVNRSKDADEPVNRAFEVIAEKSG
                     LKFNTVRNYYYRYIHEKDNKQGTPDMDHGEASKGSEVAGTTFTVDEVKGLMTAMLTGQ
                     AEGKSVRGCANELARNDKRLLLRYQNKYRNVISGNPEYVKGLMKQMSAQGLMYYDPFR
                     KQVVRGRNTTPEGELLDSIGRLVSNINEIRGSNLDGLLEGLQELSEMAVQFQRQYNIG
                     AGARQLPDYDDLVAINRYFLKLTPAGKLTGLSDYIRQLEDCIDGMEASQQSQ"
     gene            8993..9850
                     /locus_tag="VFD89_07420"
     CDS             8993..9850
                     /locus_tag="VFD89_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZJ58049.1"
                     /translation="MDRYGTTKRVAAWGIGGNLFLLVVKLIIGFASGSQAMIADGFNS
                     AGDVFTSIMTYLGNRISSLPGDQDHPYGHGKAEYIFSMIISFSLLLVTYQIFKNSLEF
                     VINKQSFMFSWQLAAVAFITIALKAILWGYTRRAGEKWDNLLIIANAEDHRNDILVTS
                     ATLIGIILGSRGIYWVDGVVGIGISLWIGFTGIRIFISAYNVLMDTNIDEKYTDRLIR
                     DIEAIHGVDHVDSINAKPIGVSFIIIVKVSVSGTMTVEEGHAIAAQIKTKIKGNKNVG
                     DVVVHVNPV"
     regulatory      10121..10305
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10502..11551
                     /locus_tag="VFD89_07425"
     CDS             10502..11551
                     /locus_tag="VFD89_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZJ58050.1"
                     /translation="MERKVSRFAALLIVMIMLVSWAVGCVAPKTPNGHGDEDREDAIG
                     QEDTDGINDEGEDKADANGEDEPEDDLTQDQEGLFPLEVVDSLGRSITIEKMPEKVIS
                     LAPNATETLFALGIGDRVIGVSQQCDYPEEAQTKDKMGDFWEPNIELIVEANPDILFV
                     GNAAPEEFLDKMDESGITVLALEGFDLEGTYGSIIDTGRAMGASQEAETLVKSMKDKA
                     ADIRQKVDSAEEPQVYFVISYGEYGDFTAGGKSFIDELITMAGGKNIAGDTEEEWPQY
                     SMEKLVEKDPEIVFSTMQAQPDGIADTPGYKELTAIKEGRLIVLDDNLINRAGPRLIE
                     GLEDLAKGMHPDLFD"
BASE COUNT         3475 a         2342 c         3013 g         2983 t
ORIGIN      
        1 attttaagac caatattaat attcttataa tgtatctgat acccatactg ctggcttata
       61 tctacgataa ttttattccc ggcatgccgg aagggtttgc gctttccttt gggctcttgt
      121 ttctggtcgt catggtggga atgtatgtcc cgtccatgat gatagctgag gaaaaggaaa
      181 agaagactct ggaggtgttg cttctatccc cggccagctc cgtagaaata tttataggaa
      241 aggggctctt gacgtttatc agcatactga taagcttcat tattctcgta gctatttccg
      301 gcagggtcgg gattgatcat ataggcgtta tcttaatcgg cgggttattg accgctgttt
      361 tctgcataca ggtggggatt atcgtaggcc tgttatctaa aaatcagatg gctacaggag
      421 tggtgggtac ccctgtatat atggcattgc tgttgatacc gatgcttggc agtatggggt
      481 ccccggtttt aaaaaagatt gcaaaagtct ttccgaccta ttattataat gatatgctct
      541 acaaggccct tggcggattc gaagggagtc aaacgctcct tgacatgggg cccgaactcg
      601 ggattctatt gggaagcata ttgattgcat tcgtgttact gctggtagta tacagaaaaa
      661 aggggttaaa ttgacgcctt aacccgattc tgtcggcaca acgaattaaa tccaaaaaag
      721 gctatgcgcc taaaattaga ttggactgaa tttgtatgaa atataatggt ataagacgtg
      781 cgaaatttat tgacagacca aaccgtttta tcgccaacgt cgaactggac gggaaaaggg
      841 agatggtgca tgtcaagaat accggaagat gcagagaaat atttgtagag gggactacgg
      901 taatcctaga ggaagggaag aacccaaacc gtaagaccag gtattcgata ataggcgggt
      961 ataaggggga tctcctcatt aataccgatt ctcaagtgcc caacgccgta gtttatgaag
     1021 ctattaccca aaataaaata gaagaggtgc aaaacgttac caaggttaaa agggaagtaa
     1081 cctttgcaaa ttcccgtttt gatatctact tcgaggacat gagcagaaaa gggtttattg
     1141 aggttaaggg tgtgaccctg gaatatgacg gggtatgcat gtttcccgat gcccctaccc
     1201 aaaggggagc aaggcatgtt catgagctga tagaggccaa aagacagggc tatcacagct
     1261 atatcatctt tttagttcag atggagggta tgaagtgctt tacacccaac tacaggatgg
     1321 atccggaatt tggcgaagcc ctaaaaactg cccatagccg gggggttaaa atcctggttt
     1381 acgatagcgt ggtaggggaa gatgaaattg ctataggcaa tagtctgggg tttcatctct
     1441 aatgcctgag gctccatgtg attatattga aggggagaag gatgatgagg aataagcggt
     1501 ctgtaatatt taagtcttta atttcgttga cgttgataat ggccttggca ttaccgggtt
     1561 acgggaacaa actatccata gcagagggaa aaattacgtc ctcggaacgt ctggtagggg
     1621 ataacgtggg tttgatagag gggaattatt acgtcctttc ccaatctgga tttatgctca
     1681 attacgagat acagaacgga accgaaaaca atataaccca aataatttta gaagagaccc
     1741 ttttcgacga aagcggccgc gtcgtaccgg gggaaggggc ccccgatacc gagtttataa
     1801 ttgaagcggg acagaagaaa acatttcagg gcaaaaaatt caaggcccag gaaaagccga
     1861 cggcctacac tctggaatat tccattcaat atattttgga agatggggag gggccgggtc
     1921 tgatctccag cggtaaaaga cagataacgg tcctgtccac ggggctcagc gtcgtatata
     1981 aagccagtac aggcggccct ataccccaag ggagtgaggt tacctacacc ttcgaaataa
     2041 aatcatcggc caatattaac atcaataata tcctggtaca ggacagcgtg ctgggggata
     2101 taggcgtaat accgattctt gcaccggacg aaaccgcaac cttgtccatg acccttaaac
     2161 ttaacgagac caccaaaagc taccccatta ttatttttga gggtccgatg gacggacagg
     2221 gcaatataaa aagggaattc aaaaacgcgg cggtagaggt agtagtagag gaaaagcagg
     2281 tggaaaaccc tttagtaatt atagggaagg ccagtaagtc caagatagcg cctaacgaag
     2341 aggtggactt tagcctgata gttgagaata aagggaacag aaccctgact aaagtcagcc
     2401 tgacggattg gacggggaaa gagatattaa ccaaggataa cctcgctccg ggaaaagagg
     2461 gggtcataat acatgcctgg agggtggaac cggggaagga gtatacattt aaggcgacag
     2521 ccgttgaaga aggtacggat cgaaaagtgc aggcatcata tactgccaaa ttttccgcta
     2581 tcgaggcgga aatggaaata gccaacaggg taacgcctca ggaggtggcc gcaggcgata
     2641 ccgtcacgat agaatacatt cttaaaaata cgggaaaggc aactatggtg gatgtgcggg
     2701 ttcaggagcc ggaatttggt gaagttggga gttttgatgt gctaaagccg ggtcaaacgg
     2761 agacctttgc aattgaaagg gtaattgaaa aggataccgg cagtcatcct atggtgtatg
     2821 cgaaggacaa agattcagga tatgagtatg agtttcaggg cgatttaatt gagatcgccg
     2881 tcaatatcgt tgagacacgt cctcttttga ctattaaact tgccacggag ccggaatcgt
     2941 taacagaggc cggagcggtg gaccttatct gtactgtgat aaacgaaggg gatgtaaaga
     3001 ttgacaatat tgatctcgtc ctaaatgaaa gagaactagg catcggaagc atcctgaccc
     3061 tggagtccgg ggatgaggaa acccttaccc tgcccgggtt ggacatcgga gaggacacat
     3121 catttacggt tacagccagg ggtgttacct acttgggcga ggaggtggag tttgtaagcg
     3181 ctccatatga aattacaata ggcggtgacg atattcccga agaacccatc aaaaacccaa
     3241 aaatcagttt cctgaaaaag ctacttggga tcattgccat cctgggcgtt gcgacggtgg
     3301 ggggtatggt ctatctcttg agggacctaa ggcgcggtgg caagaaaaag acagtcggtg
     3361 gtgggagcaa acaaagggca agaaaaagga agaccactcg gaaatagata cctgtttttt
     3421 aatctgccgg gtatgaatgg gcaattgcta atgcgtatat ttattcacca attgatccag
     3481 ggacatccag tattgataac ccttacgcct ccgttttttt ctagatgcag tttttgtatc
     3541 ccccggagag aacacccatt ttagaaaggt catccaagct ataattatct tggagcccat
     3601 atcctcatcc ataggctcca atgcgaagcc caacctcttc atggcagggc ccatcatggt
     3661 aacaccccag actgctttaa ccttggggtc cagctttccc cgctgtgcca agcaggctaa
     3721 atgggcaatc tcttcccgaa aaaggcgtag aagctggatg tcggaagcga cctctacccc
     3781 ggccaccacc ccttgggcga gggtcagatt ggaaatgtgt atctccagca catagtcacc
     3841 ggggctaacc tcggtcccgt cgtctaagga agtaggtcgg cccttgtatg ggtgtaccga
     3901 cattctcaaa aggccatagg gtgaattttc tatgggattt atgttttctg cccacgagaa
     3961 aaacttttcc cagagggtta taacgggtct gattattgat cttaacatgg tgtaccccct
     4021 aaatttattc taggcttgca aagctgtatc cgtctttacg aagggatcta attatcatgg
     4081 gtagggccat aagcgtgttt tccggagccc cgggatcccc ttgattgtcg tgtagcacta
     4141 tgacagagcc cgggactgcc ttatccacga cggtttcatg tatatgttct gcggcggtat
     4201 ttatatccca gtcctgagcc tctatggacc aataggctat accgagcccc aacctcccgg
     4261 cagcagctat actaaagaga ttaaatcccc cccatggggg gcgataccat tttgggaata
     4321 tgcccgaggc aagggatatg gcctcatagg cgcgggacat gtccttaaag gttaggacgg
     4381 gggacataag ccaggcatgg cgatgggaat aggaatgaca ccctatggta tggccttcct
     4441 ccgctatgcg tttcactata tcaggataaa gctccgcttg cctccccatt aaaaagaaag
     4501 tagcctggat ttggtgctcc ccaagaatgt ccaacaatct gggggtgtaa tccggattgg
     4561 gcccgtcgtc aaaggtaagg tagatttttc ttttatttga ggagcctccc ctgagcacct
     4621 tggatgagca gttacgtgcc cagtaattgg gcagaaggca atatatcaaa aaaatgatcc
     4681 ataggacggc tggaaatagc caccacatat tttcacttcc cttaagcaat atttaatctc
     4741 tatagcggcg tcaatgggat ctgcctttag gcgtacctat cctttttttc caacgaaatc
     4801 tcatcaccgg gttcaatata atgatcatgg gatacgagtc ctacaattgc gctgaccgca
     4861 tgtctggcgg atgagggctt tgaaagatgg gccatagcat gggatatgtt ctcgaggaca
     4921 gaagggtcct ctacggcatt tttaatgagt tccaccacct gattttcatt ttgggcaata
     4981 agagccgccc cattgtctaa caaaaagttt acgttaccca tctcatgccc cggaaggggc
     5041 ttatacagca ggattggaag cttaaccgct agggcctcaa aaaccgttat tccgcccgcc
     5101 ttcgagagaa gcaaatggct gcccctcata acaggggcga tattttctgt atatcccagg
     5161 accctaacgg gaacaggaga gcgccgggcc acctcttcca ggttcttggc aagggccctg
     5221 ttggaaccgg ccagggcgac tatctgcatg ggcagtccca tatcccctag cctttcacat
     5281 attctttcga tacctggcat aagccccgca cctcccccca taattagcag agtaaatagg
     5341 gactgatcca aattatgtct aatccagata ccgggatccg ggacaggggc ttcgaattgt
     5401 gatcttatgg gtatacccgt aacatgtatg ttttttgcag gaatgccttt ttctataagc
     5461 aactgggaga ccacatccgc agccactatg taggcatcag ccatgggatg tatccattgg
     5521 ccatgggcgg taacatccgt aataacgaca gctaggggaa tatcaatctt accctctcgt
     5581 tttagctgcg aaactacccc ggcgggggtg gggaaggtgc aaataataac cttgggggaa
     5641 tgctccaaga tcaattcgta tattctcttc ctacccatac tgttcaacat ttgttgccat
     5701 ttagagcggg aatctgtttc ccgggttatt ctgtaaaacc aatcatatcc ccaggaaaaa
     5761 tgccggatca tttgggaata gcagaacttt agggacttat taaacaaggg gcctatatac
     5821 tgaaagtaat tatatattcc aacctcccaa tggggacggc gctccttgaa aacacctttc
     5881 agggcaacgg aaacctggtg gtggcccgta ccaaaatcag cagataaaat caacacatca
     5941 cattttttaa agtcttcttg ggacatccat acactcccct aaccgtcact tatcatctat
     6001 atattttacg acagacattc tacattggaa tgcgggtgtc ataaagcatt atctttaaat
     6061 ccataagtta tgaaaaatac attgttctac tacaaaagca tgtagactaa ccgaatctgt
     6121 aatgaagatt ttttcacacc atatctatat ataaaattcg actatgcccg ttttcggaac
     6181 taaaccatta taccaaagta tatatgggtt tgtacaccga aaatctgttg gggggctata
     6241 agccattgat atctccaagg tcctaggcac cggaaattta ctataggggt gcaaaaaaca
     6301 aacaactcca actatgaaat ttatcgtcat gaaattgcac aaacccattg caattgtcat
     6361 accgatatat ttagtttacg tattgtttat ctataatttt tgatggtata tcacaatata
     6421 aatactgtct atgatataat tgtacggtgg ttatcattca gatagcccgt tttaagggag
     6481 gggagaccgc atgaatatag aatcccaatt gatcgagagg tcagttagcg gtgatacgga
     6541 tgcttttgga gagctggtat tattatatga caaaaggata tataattact gctttcgtat
     6601 gaccaacaat gccgatgatg ctgaggattt ggcccaggag gtttttataa aagtgtatag
     6661 aaatctgggt tcctttaagg ggaattgcaa attctctacc tggatttata gaattgcaca
     6721 taatacctgt atagataatc atcgaaagaa gaggtttagg cttttgtccc taagcctgca
     6781 gggggatgaa aagggagaaa tggatattcc tgacaaaggg ccgtcacccg aagaacaaat
     6841 agtaagccgt gagcaatatg gtcttatgaa ggaatgtata gcgagattaa aaccggaata
     6901 taaatccgta atcatattga gggatataca aaattatacc tatcaagaga tttccgatat
     6961 tttggaaata cccctgggaa cggtaaagtc caacataagc cgcgctaggg ccttacttcg
     7021 ggatacctta aaatcccgtt tggcggcgta tggtgaaagg agggtaaatt gatggattgc
     7081 agggattttg actattatat aagcctttat atcgataaaa tgttggatga gggggataag
     7141 gaaaagcttg taaatcacct aaaagagtgt cagagatgca gtgaaggctt tcggaaaacc
     7201 caacaaatcg tttccatgat aaatagcttg gaatcggagc cgttacccca tgactttaac
     7261 gataaattgc tgggcaagat aggacacgga aagaggatag catcgcccca ttggttaagg
     7321 tggacaggcg ccgtggcagg aattttagtt atattttttt caataaagat agccctggat
     7381 gtaggcgtta cggacaaggc atcgtccacc gcgaccctca cagaagggat gaaaggggag
     7441 gatatagcag atggctcaga gttaatgcaa atggaatctc cgggggaggc cagggatgac
     7501 ttagaggcag acagcgccca atacgatggt gtggaagata gtgtggaaag agagggcatt
     7561 ggggatggct atgacgaaga gatggtaagc aaagaggatt cccaattaaa cgatttgccc
     7621 cggatacaat cgaatgaagt ggaaatatac gtggaggata tatgcataac cccggaaacg
     7681 ctgaagttta tagcgataga taatgaactg gaactcatcg aaagcggcga aggtttcatt
     7741 gttatcggga ttcatagtca aaaggaaagg gatattcttc accgtgagct atccaaacta
     7801 ggggaggtcc gggaggtagg ggaagaaatg gacaccgatc aggttaagct aattataagg
     7861 gctgccgaat gatggagtgg atttaaaacc aacaaaattg gatacgggat ttagctgtat
     7921 aaataggata attatggtga ataatatatc cataacacat aaaccagagg agatgaggtt
     7981 aatgcaggat aatacggcta aatccttttt agatcccatt ctgatatccc ttatgaaaaa
     8041 ggaggtcaac agatcaaagg atgcggatga accggtaaat cgtgcctttg aagtgatcgc
     8101 agagaagagc gggctcaaat ttaacaccgt aagaaattat tattaccggt atatacatga
     8161 aaaggacaac aaacagggga cccccgatat ggatcacggg gaggcatcaa aaggttcaga
     8221 ggtagcaggt actactttta cagtagacga ggtaaaaggg ctcatgacgg cgatgctgac
     8281 aggtcaagcc gagggtaaat ccgttcgggg atgcgccaat gagctggcga gaaatgataa
     8341 aaggctcttg ctgagatacc agaacaagta cagaaatgtg atatctggaa atccagaata
     8401 tgtaaaggga cttatgaagc agatgtctgc ccagggattg atgtactatg acccatttag
     8461 aaagcaggta gtccggggac ggaataccac acctgaagga gaattgctgg atagcatagg
     8521 tcgactggtt tcaaacataa atgaaatccg aggttccaac ctggacgggc tgcttgaggg
     8581 actccaagaa ctaagtgaaa tggctgttca atttcagcga cagtataata taggggcggg
     8641 tgccagacag ctacccgatt atgatgatct ggtggccata aacaggtatt ttcttaaact
     8701 cacacctgcc gggaagctga ccggtctatc ggactatata agacaactgg aagattgtat
     8761 cgatggaatg gaggcaagcc aacaatccca atgaaattgg aatgtatcat ttcctttttg
     8821 ggattataag gaggcaccca tcccttgggc aacgcatcca cagggattgt cataaaatgg
     8881 gcttgcctgc cgctgtatgt ttgggtatga tattgctata gtgcttgatt tacgccgtga
     8941 aatgggaacg cccgtgtttt tcgcggcgta tgatttagtg gggggtgttt gtatggaccg
     9001 ttatggcaca accaaaaggg tcgccgcatg gggtatcgga ggcaacctct tcttgttggt
     9061 tgtaaagctg atcataggat ttgccagcgg gagtcaggca atgatagccg atggctttaa
     9121 tagcgccggc gatgtcttca cctcaattat gacctaccta ggtaatagaa tatccagttt
     9181 gccgggggat caggaccacc cctacggaca cggcaaagcc gagtatatct tttctatgat
     9241 tattagcttt tcactgcttt tggtaacata tcagatattt aaaaactcat tggagtttgt
     9301 gataaataag cagagcttta tgttttcttg gcaactggca gctgtagcct tcataaccat
     9361 agccctaaag gcgattttat gggggtatac ccgcagggca ggcgagaaat gggataacct
     9421 attgattatt gccaacgcag aggatcatag aaatgatata ttggtcacct ctgctaccct
     9481 catagggatt atcctcggga gcagggggat atactgggtt gacggagtag tcggtattgg
     9541 tatttccctg tggatagggt ttacaggtat tcggatattt attagcgctt acaatgtgct
     9601 tatggacacc aacatcgacg aaaaatacac tgacaggctc ataagggata tagaggctat
     9661 acacggcgta gatcatgtgg attccataaa cgccaaaccc ataggggtaa gtttcatcat
     9721 catagtaaag gtatcggttt ccgggaccat gaccgtagag gaagggcatg ccatcgcagc
     9781 ccaaatcaag acgaagataa aaggaaataa gaacgtgggc gatgtagtgg tgcatgtgaa
     9841 tcccgtctga ttaattcgtt atgccgccta tatatgtggt gatataaaat tgtgtcataa
     9901 ccctttgtca gctgaatcat attatcatat gtacaactgg tttatatgac aagtcgccca
     9961 ccaaatttaa gaagataacc actggaaaac cttagggata gatgatatac tctacaggta
    10021 agggggcgtg ctattaagga catagtctat ttggcatgca gcatgcccaa acaagcgatt
    10081 aatatatcgc aataaccaat gcaaggcgtt ataaaattaa acagcattta tatccatagg
    10141 tgtccgggat accggattaa aagggaaagc ggtaaaaagc cgctgcagcc cccgctactg
    10201 taaatgagga catgtttact gaaataaacc actgggaaac tgggaaggtg cgtaaacatg
    10261 aatgattcat aagccaggaa acctgcctat gggtatgtaa aagctagttc cttcggaggg
    10321 aaggattggg agcttatatg tttattttaa aatccaatcc aattcaattg gatttttatt
    10381 ttgggttgga tcatattcca agttcccctc ctaaagcgaa tttcttgcct atggtgtgaa
    10441 tttgactaaa aggcgggtat ccgcgattta gatcgtcttg caaaatatgg agggattttt
    10501 tatggaaaga aaggtttcaa gattcgctgc acttttaatc gtaatgataa tgttggtctc
    10561 ctgggcggta ggatgcgttg cgccaaagac tcctaacggc catggcgatg aggaccgaga
    10621 agacgcaatt ggacaagagg atacggatgg gatcaacgat gaaggggaag ataaggccga
    10681 tgccaatgga gaagacgaac ctgaagacga tttgacccag gaccaagagg gtctatttcc
    10741 tttggaggtt gtagatagcc taggtagaag tataaccata gagaagatgc cggagaaggt
    10801 tatatcattg gcccctaacg ctacggaaac gctttttgcc ctaggcatag gtgatagagt
    10861 gataggcgta agccaacaat gcgattatcc cgaggaggcg caaacgaagg ataagatggg
    10921 ggatttttgg gagcctaaca ttgagcttat agtggaagcc aaccccgata tcctatttgt
    10981 aggaaatgct gctcccgaag agttcctaga taaaatggat gaaagcggga taacggtact
    11041 ggctctggag gggttcgacc ttgagggtac atacgggtct ataattgata caggtagagc
    11101 catgggagca tcacaagagg ccgagaccct ggtaaaatcc atgaaggata aagccgctga
    11161 tatccggcaa aaggtggaca gcgccgaaga gccccaggta tatttcgtca tttcttacgg
    11221 tgaatacggc gattttactg cggggggcaa aagctttatc gacgaattga tcaccatggc
    11281 ggggggtaag aatatagccg gggatacgga agaggagtgg ccccagtaca gcatggaaaa
    11341 gctagtggaa aaggatccgg agatagtctt ttctactatg caggctcaac ccgatgggat
    11401 agccgacact cccggataca aggagcttac cgctataaaa gagggccggt tgatcgtgct
    11461 ggacgacaac ctcataaaca gagctggacc taggctgata gagggtttgg aagacctggc
    11521 aaagggcatg caccctgacc tgtttgatta ggcttttgaa ctgttatact ggtttcatac
    11581 cccggtagtg ttgtaatagg gcgcattaaa acaccgttgt cattccgggc attgcctgga
    11641 aacttccgag gtctttggct ttgcctaaag tgacatgttg tttgatgcgc tttttaaatt
    11701 ccatctttgg gggttaaaga tataggggga tagggcatat gatttatatc cataggagaa
    11761 ggcattatag gaaggtctat gttgccttgc tcatatcgct gtttatagtt att
//
LOCUS       DASRNX010000185        13981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_302613,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000185 DASRNX010000000
VERSION     DASRNX010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13981
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302613"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFD89_07430"
     CDS             complement(<1..564)
                     /locus_tag="VFD89_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HZJ58051.1"
                     /translation="MKRFLLILLSALLILSLFAGCAKSGDDKKILVGASPTPHAEILE
                     IAEEELESKGYELEVKEFTDYVQPNMTLDSGDLDANYFQHKPYMDDFNEKNNTKLVSI
                     AAIHYEPFGIYPGKTQTIDELPDGAQIAVPNDATNEARALLLLEAQGLIKVDPDAGLE
                     ATVIDITDNPKDIKILEIEAAQLARSLP"
     gene            complement(817..1476)
                     /locus_tag="VFD89_07435"
     CDS             complement(817..1476)
                     /locus_tag="VFD89_07435"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HZJ58052.1"
                     /translation="MFEPQIVSLIGWGFLETLYMTLVSTLIAYILGLPLGIALIVTDK
                     DGIYPKRYFNLVLGIIINLLRSVPFLILLILVFPITRFVVGTTIGSNATVVPLVIAAA
                     PFIARLVESSLKEVDGGVIEAAESMGASPAQVIYKVMLPEARPSLILGSAIAVTTILS
                     YTATAGIVAGGGLGDIAIRYGLYRYKTDIMMVTVAILVIIVQILQEIGMKLAKIGDKR
                     K"
     gene            complement(1469..2482)
                     /locus_tag="VFD89_07440"
     CDS             complement(1469..2482)
                     /locus_tag="VFD89_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZJ58053.1"
                     /translation="MIRNPIIEIESLSKTFTSTNGEFHALDNINLQILEGEIFGIIGM
                     SGAGKSTLVRCINFLEKPTQGTVFFNGRDLSRLTKRELYSIRQSMGMIFQQFNLLMQR
                     NVMGNICFPLEIAGMDKRSAKMRAQELLELVGISEKAYSYPAQLSGGQCQRVAIARAL
                     ATNPKVLLCDEATSALDPATTQGVLTLLKDINKRLGITIVVITHEMRVIQDICNRVAI
                     IDHSRIAEVGDVEKIFSNPKTAAAKRLVFPQDQSIRHFSGTRCLRIVFDGNSSFEPVI
                     AGMVLDFRQPVNILFADTKNIDGRAFGQIVIQLPQDESVADGMIEYLRAKNLTVEEVE
                     GYV"
     gene            2985..3485
                     /locus_tag="VFD89_07445"
     CDS             2985..3485
                     /locus_tag="VFD89_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZJ58054.1"
                     /translation="MNVIFKRRSIRAYTDRTVPDELIEKVLRAGMAAPSAGNQQPWHF
                     VVIDDRNTLNEIPKIHPHSDMLREASHAIVVCGDIPSQKYEGYWVQDCSAATQNMLLM
                     ATELGLGSVWLGVYPIEDRIEALKNLFGMPDEVIPLCIVSIGYPAETKEPEDRFDASR
                     IHKNKW"
     gene            3510..3965
                     /locus_tag="VFD89_07450"
     CDS             3510..3965
                     /locus_tag="VFD89_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZJ58055.1"
                     /translation="MDKSVLGTRVVTQVAVLVNDIEKACQDYADFLGVENPGYSLTDT
                     IDKTQAEFRGEPCNARAKLAFFPVGENVTIELIEPDKNPSVWREDLDKNGEGFHHIAF
                     VIDGMKEKVMLLEKNGMPLVQKGEYTGGRYAYVDAREKLKLMLELLEND"
     gene            4421..5641
                     /locus_tag="VFD89_07455"
     CDS             4421..5641
                     /locus_tag="VFD89_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019686048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HZJ58056.1"
                     /translation="MSELINNREYRQKVLKELIMELHDGKPVEKVRERFAKLIEGISV
                     AEISEMEQNLIMEGMPIEEVQRLCDVHAAVFKGSIEDIHKPTSMEEIPGHPMHTFKWE
                     NREIEKKLKSGAEPALADFKGSDSSENINNLLQNVAELLEIDKHYSRKENLVFPHLEH
                     AGITAPPQVMWGVDDEIREKLKEAHALLSDYKGNKEEVVQTVEGVVGDIREMVFKEEN
                     ILFPLMAETLSQDEWLTVYRDSDEIGYTFIAPKAKWVPERADYVDDTSPSHIDSDVIR
                     FDSGVLTAHEVETLLNHLPVDITFVDKEDRVKYFSQSSERIFTRPKSVIGRTVQNCHP
                     PASMHVVNQIVEDFKNGVKDHEDFWIRMGEKYVLIRYFAVRDEDGEYMGTMEVSQDIA
                     PFQKIQGERRLLSE"
     gene            complement(5643..5906)
                     /locus_tag="VFD89_07460"
     CDS             complement(5643..5906)
                     /locus_tag="VFD89_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58057.1"
                     /translation="MKVLMKPIEMIAWFTQDGLPTPIRFRMEQPDGSYMVIKVAQTIS
                     RKEERLAGNPMIIFTCQSVVHNMERLYEIRYEIRTCKWYIYKI"
     gene            6600..7520
                     /locus_tag="VFD89_07465"
     CDS             6600..7520
                     /locus_tag="VFD89_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZJ58058.1"
                     /translation="MKPKENYVINVLNQRIDLENRVPFRAKDTMEITLDAFDARSDTA
                     IYWLGGGGFLLNSRGTILMVDPVLEMDKELMKCETGHNMLMPYPISASMVPKADCVLY
                     THPDLDHVGPTTSQILSQKGIKRIAPSPVAIKLIEYGADIDDITVCRSGDRYRVGNLE
                     IEVIKADHPWQLINTKLFGKPLRGDDACGFVVRTPDGSFLFPGDTRLLESHLDIPSID
                     VILVDTSKCLFHLNVEGAIILCNSYPDAYLIPYHYGMFDEPQTPAQEQGDPKGVFKAV
                     HDAEDRAFTLAPGEPFVLAEGRAELKDNDL"
     gene            7622..8863
                     /locus_tag="VFD89_07470"
     CDS             7622..8863
                     /locus_tag="VFD89_07470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZJ58059.1"
                     /translation="MIKRALKDRPEIFIFFVMIAALSLGNALSDGVYSNYFKEVYNVT
                     AFQRGLIEFPRELPGLLCAFVIGTLGFLGDLRVAFIAQILAIIGVTLLGLFAPVFGIM
                     LIFLFINSMGMHLLMPLQDSIGMALAEKDKIGRRMGQYSSVRAALGLVAGILVFFGFR
                     IGFFTFNTTVKWIFLVSALAFVVALIMTAVMIGRIKPRRLAPQKVRLVIKKEYRHYYL
                     LTILYGVQKQVTGVYSTWVIVDLLLKKADTLALLSIVVGFISIFFLDKIGKWTDKFGI
                     KHMMYADALIFIGIYAIYGFVVWGITSKALPSQGLSIWIVYLLFVLDKLSMQMGMVRS
                     VYLRSITTDEDELTSTLSMGVSLDHLVSIFAAIAGGYVWSKWGSQWVFFLAALFSLGN
                     LYVAHRIRREEQSHILSMEIP"
     gene            complement(9064..10311)
                     /locus_tag="VFD89_07475"
     CDS             complement(9064..10311)
                     /locus_tag="VFD89_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZJ58060.1"
                     /translation="MNNRIIFHIDVNSAYLSWEAAYRLQHGSTLDLRDIPSAVGGDAT
                     TRHGIILAKSIPTKPFKIQTGETLYSARLKCPHLVVVPPRYHIYMQCSKAMVSVLQEY
                     SPSIQRFSIDEVFLDYTNMETHWGHPIEAAHRIKGRIRDELGFTVNIGISGNKLLAKM
                     ASDFQKPDNVHTLFIDEVQKKMWPLPVEELFMVGRATTSKLRNKGIFTIGELATTDPE
                     LLQQWLKSHGRLIWEFANGIEDSPVGGGPPVKGLGNSTTLPFDVEDRKTGHMTLLSLT
                     ETVAMRLRDIGRSAGVLSVSIKDRDFVSVSRQKKLDVPTHSTGIIYKNACTLFDELWK
                     GQPLRHMGLRASDLYSDDLYQLSLFEADIENQRALDTAVDEIREQYGIDSIIRSCFLH
                     SGLNPMTGGVIQEEEYPMMSSIL"
     gene            complement(10766..11080)
                     /locus_tag="VFD89_07480"
     CDS             complement(10766..11080)
                     /locus_tag="VFD89_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZJ58061.1"
                     /translation="MFYDAHILIWDICRKTYRLPAKIGEFMYSIEKQICLDRLVQVQD
                     FVILASKYECDVKVKSKNSITDGKSILGILSLALWHPVTVSAYGADAKQFAAGLTKFR
                     SH"
     gene            complement(11292..12089)
                     /locus_tag="VFD89_07485"
     CDS             complement(11292..12089)
                     /locus_tag="VFD89_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZJ58062.1"
                     /translation="MKIEHDLHVHTHLSSCSDDPAATAENYIKLARDRGLKLIGFANH
                     FWDSNIPGASEWYKPQDFDHIMRIKEELPQDISGIKVFIGCETEYCGGGKVGITKEVA
                     EQLDFVLMPMSHLHMEDFVRDKDITAPKDVAKLMVDRFNEVLELDLATGIAHPFIPLG
                     FSNVDEILSHISDDEFRNCLGRAAELGVSIELNASSFPGNYGRETDLFHDESLIRAFS
                     LAKECGCLFHFASDAHSLKSFENFFAMERTINILGLTEEDILPLCRV"
     gene            12566..13792
                     /locus_tag="VFD89_07490"
     CDS             12566..13792
                     /locus_tag="VFD89_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HZJ58063.1"
                     /translation="MDKIRLARPTKPQMMWQDLEMGMFCHFGINTFCDQEWGDGTDDP
                     MVFNPSSLDARQWVKTAKSAGFKYFILTAKHHDGFCLWPTKTTDYSVKSSPWKDGRGD
                     IVKECAKACREEGMLFGLYLSPWDRHEPRYQDKDAYDDFYAEQLTELLTGYGPLVEVW
                     FDGAGSEGREYDWPRIIGLVKKYQPETMIFNMGAPTIRWVGNEDGVAPYPCWNTAEDA
                     RISMFTDDTVKWDTDTPLWLPAECDVPIRRRQWFWHPDAEENLLSLEDLKNIYYRSVG
                     HGTNLLLNIAPDDRGLIPDSDAKRAMEFGRWVQETFSDPIKEISGAGDRLEIRLDSPQ
                     TISHIILMEDIEHGERVRDYAIDYRKDGRWVELVCGSAIGHKKIDRFEPITTDHVRIR
                     ILETADKPIIRKFALV"
BASE COUNT         3940 a         2978 c         3291 g         3772 t
ORIGIN      
        1 gggtagggaa cgcgcaagct gtgcggcttc aatttccaga attttaatgt ctttaggatt
       61 atccgtaatg tcaattaccg tagcctcaag gcctgcatcg gggtcaacct tgataagtcc
      121 ctgagcttcc agaaggagca aggcccttgc ctcgtttgta gcatcattgg gtacagctat
      181 ctgggcaccg tccggcaatt cgtcgatagt ctgtgtcttg ccgggataga taccaaaggg
      241 ctcatagtgg atggcggcga tggatacaag cttggtatta tttttttcat taaagtcatc
      301 catgtagggc ttatgctgaa aatagtttgc gtccaaatcc ccggaatcca gggtcatatt
      361 gggttggaca taatcggtaa attctttgac ctcaagttca taacccttac tctccaactc
      421 ttcctcggca atctctaaaa tttccgcatg tggggtaggg gaagcaccca ctaaaatctt
      481 tttgtcgtcc ccggactttg cgcagccggc aaacaatgat agtatcaaca gcgcgctcag
      541 cagaataagt aaaaaccttt tcatagacct gacctcctaa ttttacgtat tggtataata
      601 ttatttaggg cgcagtgaga catctgttaa ataaccccac cgcgctctaa aattcattag
      661 ttttattgtg caaggataca acgacccggc accgtaggtt aaaacaatat attctcggca
      721 acacatggat aaatgataat gttttgcgat ataagcgggg atatgcgggg atatgacgta
      781 tttatcgtaa aacatatagg caacagatca tagcagttat tttctcttat ctccgatttt
      841 ggccagcttc atcccaattt cttgaaggat ctgtactatt atgaccaaaa ttgctaccgt
      901 taccatcata atgtccgttt tgtatctata aagtccgtac cttatggcta tatcccccag
      961 gccgccgccg gctactattc cggcagtagc ggtatagctt aaaatggtgg tcaccgcaat
     1021 agcactgcct aggattaaag acggtctggc ctccgggagc ataaccttat agattacctg
     1081 tgcaggggat gcgcccatgg actcagccgc ctcaataact ccgccgtcca cctctttcaa
     1141 ggaggactct accagtctcg ctataaaggg agccgctgca attaccaggg gtacgacggt
     1201 cgcattggaa ccgatagttg tacctaccac aaaacgggta atgggaaaca ccaaaatcaa
     1261 caggatcaaa aagggaactg atcttaaaag atttatgatg ataccaagaa ccagattaaa
     1321 atatctcttt gggtatatac cgtccttatc cgtaactatt aaggcaatcc caaggggcag
     1381 gccgaggata taggctatca aagtagaaac caatgtcata tacagggttt ccaggaatcc
     1441 ccatccaata agacttacta tttgtggctc aaacatatcc ttcaacctcc tccaccgtta
     1501 aattctttgc tcttagatat tctatcattc cgtcggcgac gctctcatct tggggcagct
     1561 ggattacaat ctggccaaag gccctgccgt cgatgttctt tgtatccgcg aataggatat
     1621 tgaccggctg tcggaaatcc aacaccatac cggctatcac cggttcgaag gacgaattcc
     1681 cgtcaaaaac tattctcaag caccgggtcc cagaaaaatg ccttatggac tggtcttggg
     1741 gaaagaccaa tcgtttagct gcggctgtct ttgggttcga aaagattttc tcaacatccc
     1801 ctacttcagc tatgcggctg tgatctatta tagccaccct gttacatata tcttggatca
     1861 cccgcatttc atgggtgatt acaacgatgg taattccaag gcgtttgttg atgtccttca
     1921 aaagggtcag cactccttgg gttgttgccg gatccagtgc gctggtcgct tcgtcgcaca
     1981 ggagcacctt gggattggtt gccaatgccc tggctatggc caccctttgg cattgtccgc
     2041 cggacagttg ggccggatag gaataggctt tctccgaaat ccccacaagc tccaacagtt
     2101 cttgagcacg cattttggcc gatctcttgt ccattcccgc tatctccagg ggaaagcaga
     2161 tgttacccat tacgttcctt tgcatgagca gattaaactg ctgaaatatc atccccatgg
     2221 attggcgtat gctgtacagc tctcttttcg taagacgtga aaggtcccta ccattaaaaa
     2281 aaactgtacc ttgggtgggc ttttccagga agttgatgca tcgtaccagg gtgcttttgc
     2341 cggcaccgct cataccgatg atcccaaata tctccccttc aaggatttgt aagtttatat
     2401 tatcaagggc atgaaattct ccatttgtac tcgtgaatgt tttgcttaaa ctttctattt
     2461 caataattgg atttcttatc atcaccccac ccccttacca tttttataaa gacttaattt
     2521 ataaccgcaa aatcacaata tatgcccccc cggacatcaa tggaaaattg atgcaggcgg
     2581 gttttagaca acggagcacg cccgtgtgta ttgatatttt gctaaagtat atgacacata
     2641 tagtaccact attacgacaa ataaacaagc ttctggacta atccgataat agcccaagca
     2701 tgttttggtt gtagcatatt gatgtagcat aggaaatcat ccaccagacc atgccgacca
     2761 gaccatgccg gctaacgata aatatagtag ccggactaat tctcatccca taaaattcgg
     2821 acatagaaga taatcgataa caatcgaata aaaggggata gggaaaacct tgaagcccgg
     2881 tactcaggta tggtacaatg ggcatggtgg tattatgatt tatagggaaa aatgtaaggc
     2941 atgggacagg attattctgg taatatataa ggaggagatg taaaatgaat gtgatcttta
     3001 agagacggag tatcagggcg tacaccgata ggactgtacc ggatgagctt atcgaaaagg
     3061 ttttaagggc cggtatggcg gcgccgtcgg cgggcaacca gcaaccatgg cattttgtcg
     3121 taattgatga cagaaatact ttgaacgaga tccccaaaat tcatccccat tccgatatgt
     3181 taagggaggc gagccatgct atcgtcgtat gtggggatat cccgagtcaa aaatatgagg
     3241 gatattgggt acaggattgt tcagccgcta cccagaatat gctgctcatg gcaacggagt
     3301 taggcttggg atctgtatgg ctgggggttt atcccattga agacaggata gaagccctca
     3361 aaaacttgtt tggcatgccg gacgaagtaa tcccactatg cattgtatcc ataggctatc
     3421 cggcagagac caaggaaccg gaagatcggt tcgatgcttc aaggattcat aagaataagt
     3481 ggtaatatta aggagaggag gtgacatata tggataagtc tgttctagga accagagttg
     3541 taacccaggt tgcagtcttg gtaaatgata tcgaaaaggc ctgtcaggac tatgccgatt
     3601 ttctgggcgt ggaaaacccg gggtattccc taaccgatac catagataaa acccaagccg
     3661 aattcagggg cgaaccctgt aacgcacggg caaaattggc ctttttcccc gtaggagaaa
     3721 atgtgaccat tgaattaatc gagccggata agaatcccag cgtctggaga gaggatttgg
     3781 ataaaaacgg cgaagggttc catcacatag cctttgttat tgacggaatg aaggaaaagg
     3841 ttatgctcct cgagaaaaac gggatgcccc ttgtacagaa gggcgagtat acaggcggtc
     3901 gttatgcata cgtagatgcc agagaaaagc tgaagctgat gcttgagctg ttggaaaacg
     3961 actgatgatg cccggcatat gaatgttttg gtacatatgc ccatttcggg ctatgccgtg
     4021 aagtttcctg tctaataaaa cctgtggtcc acacaaagtt atgtgggccg caggttttta
     4081 ttgtaatcgg atatcctatc cgtaattgcg aataatatat atgattcaaa taccctatag
     4141 gggttattta tgctatagat agacaacttt aatgataaaa tttatcacaa tgaaattgca
     4201 tagatccgat gcaattatta cgccgatgaa tttattttct gtattgttta tctgcatagg
     4261 aagcgcgcgt tgtgccggat aagcgcatac cggcaatggc tattgggcgc tgattcatat
     4321 ttagagataa cccattactt tcatcatgca tcagggtaat gcatcgacac aagaggttaa
     4381 gaggatttac agccaaaaga caatggagga ggataaagag atgagcgaac ttataaataa
     4441 cagggaatat aggcaaaagg ttcttaagga attaataatg gagctacacg acggaaagcc
     4501 cgttgagaag gtcagggaac gctttgccaa attgattgaa ggtatatccg tagctgaaat
     4561 ctcggagatg gagcaaaacc tcataatgga agggatgccc attgaggagg tccaaaggct
     4621 gtgcgacgta cacgcggcgg tcttcaaagg gtccatcgag gacatacata agcctacttc
     4681 tatggaggaa atcccgggtc accccatgca tacattcaaa tgggagaata gggagataga
     4741 aaagaaactg aaatccgggg ccgagcccgc attggcggat tttaagggct cagacagttc
     4801 ggagaacatc aacaatttac tccagaacgt agcggagctt ctggagattg ataagcacta
     4861 tagccgcaaa gaaaacctag tatttcccca tctggagcat gccggaataa ccgccccgcc
     4921 ccaggtgatg tggggagtgg atgacgaaat ccgagaaaaa ctcaaagaag cccatgccct
     4981 cctcagtgac tataagggga acaaagaaga ggtggtccaa acggttgaag gggtagtcgg
     5041 ggatatcagg gagatggtat ttaaggaaga gaacatactg tttccattaa tggccgaaac
     5101 cctgtctcag gatgagtggt taaccgtata tagagatagt gacgagatag gttatacatt
     5161 tatagcacct aaggcaaagt gggtaccgga aagggcggat tatgtcgacg atacctcccc
     5221 atcccatatt gattccgatg taatccgctt tgacagcggg gtgttgacgg cccatgaggt
     5281 ggagaccttg ctcaaccatc tgcccgtaga catcaccttt gtggataaag aggatagggt
     5341 aaaatacttt tcacagagca gcgaaaggat ctttacaagg cccaagagcg taatcggaag
     5401 aacggtgcag aactgccacc cccccgccag catgcacgtg gtaaaccaga tagtggaaga
     5461 ttttaaaaac ggggtcaagg accatgagga cttttggatc agaatggggg aaaagtacgt
     5521 cctaatccga tactttgcag taagggacga ggatggggaa tatatgggga ccatggaggt
     5581 ctcccaggat attgcgccct tccaaaagat ccaaggggag agaaggctcc tgtctgaatg
     5641 aactaaattt tgtagatata ccacttacag gtccttatct catagcggat ctcatagagt
     5701 cgctccatat tgtggacaac gctctgacag gtgaatatta tcatagggtt gccggccaat
     5761 ctctcttcct tgcgagagat ggtctgggct accttaataa ccatatagct tccatcgggc
     5821 tgttccatcc ggaaacggat tggggtgggc aacccgtcct gggtaaacca ggcaatcatt
     5881 tctatgggct tcattaggac tttcatatat atcaccacaa ttggtcggta tcgccccgga
     5941 gaaaattata ttccggggga tagccgttta tataatcagc cgggcctacg gccatattct
     6001 atactatggg ggtccggttg attttatttc ctttaatata ctatactttc ccaaaaagaa
     6061 caagtgttct ttttgtatcc gagtgcctcg gataccagag ggacgtttcg tcagtcatca
     6121 ttcgccatgg caccaaggga ataccctgtc tttcttcgga agtgttacat ttgctatgcc
     6181 aactgaacga aataaaaatg atgcatggaa gtacatccat tatagttgta tttatcaatt
     6241 cctaatgtat aatgtaggta cagataaaca actccaacta aaaatttatc accatgaaat
     6301 tgcacaaacc caatgcaact atcacgctga taaatttagt ttccgtattg tttatctgta
     6361 gattatgatt tgggcgtcaa aattacactt ttctggcaag atacaccgta cataatgaag
     6421 ggcatagagg cccagctcat atatggtagt gcgattttgt attatgccct ttcgacgcct
     6481 gaatgatttc atataaccaa gctagatttc aaagtggaat gatccccgtt taaaattgtc
     6541 ctgtggggat agggatgaaa ctgtttaatc cataacttta tgtaaaaggg ggtttgggta
     6601 tgaaacctaa agaaaactat gtaatcaacg ttttaaacca aagaatagac ttggaaaacc
     6661 gggtcccatt tcgtgccaag gataccatgg aaattacatt agatgctttt gatgcccgaa
     6721 gtgatactgc catctattgg cttggcggcg gcggatttct attaaattcg cggggaacca
     6781 tcctaatggt agatcccgta cttgagatgg ataaagaact catgaaatgt gaaacaggac
     6841 ataatatgct tatgccatat cctatctcag cttctatggt accaaaagct gattgtgtat
     6901 tgtatactca tcctgattta gaccatgtag gccccactac atcccaaatt ttatcccaaa
     6961 aaggcatcaa gcgaatagcg ccttcccctg ttgccattaa gcttatagaa tatggggcag
     7021 acatagatga tatcaccgtc tgtcgctcag gggatagata cagggtcgga aatttagaaa
     7081 tagaagttat aaaggctgac catccatggc aacttatcaa tactaaatta tttggaaaac
     7141 ctttaagggg cgatgacgcc tgcggttttg tcgttcgcac acctgacgga agctttctgt
     7201 tccccgggga taccaggctt ttagagagcc atctggatat tccgtcaata gatgtcattt
     7261 tggtggacac ttccaaatgc ctttttcatt taaatgttga aggggctata atcctctgta
     7321 attcataccc cgacgcctac cttattccct atcactatgg gatgtttgat gaaccccaga
     7381 ccccggcgca ggagcagggt gatccaaaag gcgtatttaa agctgtacac gatgccgaag
     7441 acagggcgtt tactctggcg cctggtgagc cttttgttct ggccgagggt agggcagaac
     7501 tcaaagataa tgatctatga tatgcccatt atccttgttg ggtagcaata tagtgaacca
     7561 gcacaataga ttaagtaata aacaataaaa tatagcagac tagcgaaagg gagttaccga
     7621 catgataaaa agagcgttaa aggaccgccc cgaaatcttt atatttttcg ttatgatcgc
     7681 cgccctatcc ctgggaaacg ccttgagcga tggagtatat tccaactact tcaaggaagt
     7741 ttataatgtt acggcctttc agcgggggtt gattgaattt ccccgggagc taccgggttt
     7801 actgtgcgcg tttgttattg gaaccttggg atttcttgga gacctaaggg tagcttttat
     7861 agcccagatc ctcgccatta taggcgttac attgctgggg ctatttgccc ccgtttttgg
     7921 tataatgctt atatttctct ttatcaattc tatgggcatg catcttttga tgccacttca
     7981 ggactccatt gggatggcat tggcggaaaa ggataagatt gggcgtcgca tggggcaata
     8041 ttccagtgtg agggcggcct tgggcctggt cgccggaata ttggtgtttt tcgggtttag
     8101 aattggattt ttcaccttta acactaccgt taagtggata tttttagtat ccgccttggc
     8161 atttgtagtt gccctcataa tgaccgccgt catgatagga cggattaagc cacgcagact
     8221 ggcgccccaa aaggtccgac tggttatcaa aaaggaatac cgccattatt accttcttac
     8281 catattgtat ggggtccaaa agcaggtgac gggggtctac agtacatggg ttatcgttga
     8341 cctgttattg aagaaggccg atactctggc gcttctcagc atcgtggtcg ggtttatttc
     8401 catcttcttc ttggataaaa tcggcaaatg gacggataaa ttcggtatca aacacatgat
     8461 gtatgcagat gcccttatat ttatcggaat ttacgctatt tatggttttg tggtatgggg
     8521 cattacgtca aaggcactcc ccagccaggg cttatccata tggatcgtgt atttgctgtt
     8581 tgtattggat aaattgtcca tgcagatggg tatggtaagg tccgtatatt tgcgctccat
     8641 taccacggac gaggatgaac tcacctctac attgtccatg ggagttagcc tggatcatct
     8701 ggtgtccata ttcgcagcta ttgccggggg atatgtgtgg tcaaagtggg gcagccaatg
     8761 ggtattcttt ttagcagcgc tattttctct gggaaatcta tacgtggccc acaggataag
     8821 gcgggaggaa caatcgcaca ttttatccat ggagattcca taaaagaatt ctaaaacagt
     8881 cccttatccc gtcacccggt gggatagggg attatttttg tgccatttgc agtttgcaaa
     8941 attaaaacat cggaagcaat tgcaacacac aggtttcaac gaggcgggtg atttgtaccc
     9001 accgccttgt tttactaatc gaaacttgtc gcttatagaa gcgggagaca tcttgccgac
     9061 tcgttataat atgctggaca tcatgggata ttcctcttcc tgtattacgc ctcccgtcat
     9121 gggatttagg cccgaatgga ggaagcatga gcgtatgatg gaatcgatgc catattgttc
     9181 cctgatctca tccaccgccg tatctaaggc cctttgattt tcgatatcgg cttcaaacag
     9241 ggaaagctgg tagagatcat cgctgtatag atccgatgcc ctcagcccca tatgccgcag
     9301 tggctgtccc ttccacagct catcaaacaa tgtgcatgca tttttataaa ttataccggt
     9361 ggagtgggtc gggacatcca gtttcttttg gcgggagacg gatacaaaat cccgatcctt
     9421 tatggataca gacagaacac cggctgatct tccgatatcc cggagcctca tagccacggt
     9481 ttcagtcagg gataataagg tcatgtgtcc cgtcttccgg tcctcgacat cgaagggaag
     9541 ggtagtagaa ttgcccagac cctttacagg gggaccccct ccaacagggg aatcctctat
     9601 cccgttggca aattcccaga taagcctgcc gtggctttta agccattgct gcagtagttc
     9661 cgggtcggtg gtggctagct cgcctatagt gaatatgccc ttattgcgga gcttggaagt
     9721 tgtagccctg cccaccataa ataattcctc cacagggagc ggccacattt ttttctggac
     9781 ttcatctata aacaaggtat gtacattgtc cggtttttgg aaatccgatg ccatctttgc
     9841 aagaagctta ttgccggaga tgcctatgtt aactgtaaat cccaattcat ccctgatgcg
     9901 accctttatc ctatgggcag cctctattgg atggccccaa tgggtctcca tatttgtata
     9961 atctaaaaat acctcgtcta tggaaaacct ctgtattgac ggtgaatatt cctgaaggac
    10021 agagaccatt gccttactgc attgcatgta tatatgataa cgtggcggaa caaccaccag
    10081 atggggacat ttcaacctgg ctgagtacaa ggtttcccca gtctgaattt taaatggttt
    10141 agtaggaatg gatttggcca ggattatgcc gtggcgggta gtggcatctc cccctactgc
    10201 cgaagggatg tcccgaaggt ctagggtaga gccgtgctga agccgatagg ctgcttccca
    10261 ggacagataa gcggagttca catcaatatg aaatataata cgattattga tttccatgat
    10321 attcctcctt cagagtaaca tcattataac agaacatatg ttcggagtaa aggacaaaag
    10381 tattagaatg ttttaaacga aatggcgtag atataagata atttaatgct tgatagatca
    10441 tgatttattt ctggtgattt ttgagttata acaccgaaag taattgcagc acacaggttt
    10501 caacggggcc ggggatatat gcccaccgcc tattttacca attggaactt ggtgcttata
    10561 gaagaggggg atatctttct gccccgctat ttggcttcta aagaaagaat gagggggata
    10621 tttagagatg attaaagatt atagagaaaa cggaatcgtg agagtgagat agatatttag
    10681 agattatcaa agattatagg gaaagggaga gatgtaggga taataaaaga aggtatctcc
    10741 aactatgaaa agtggtgatg cccctttaat gggagcgaaa ttttgtaaga cctgctgcaa
    10801 attgtttggc atctgcacca tatgcggata ccgtcaccgg atgccataat gccagggaaa
    10861 ggatccccaa tatgcttttg ccatcggtta tggaattttt acttttcacc tttacatcgc
    10921 attcgtattt ggatgcaagt atgacgaaat cctggacctg gaccaaccga tcaagacata
    10981 tctgtttttc tatactatac ataaattcac ctatctttgc tggtaatcta tatgtcttac
    11041 gacagatatc ccatattagg atatgggcat cgtaaaacat tatccggaaa aagcccccct
    11101 gcatctggga tgttgaaatc ttgatctata cagaggacct tggatattga tattatacca
    11161 ccatattaag ggatttaggc caagtttgca ccagaatcaa aataatttta cattttgttt
    11221 caaaaagacc ggaaaatttg tgcactatat atagtattcc aacccagttc cgggaccata
    11281 ggcatatctt tctaaaccct gcacagagga aggatatcct cttctgtcag gccaaggatg
    11341 ttgattgtcc tttccatggc gaagaagttt tcaaagctct tcaaggaatg ggcatcgctg
    11401 gcaaaatgga ataggcatcc gcattctttg gccaacgaaa atgccctgat cagggattca
    11461 tcgtgaaaca aatccgtttc cctaccatag ttaccgggaa aactgctggc attgagctct
    11521 atgcttacac ccaattctgc ggctcttcca agacaatttc taaattcgtc atcggatata
    11581 tgggataaga tctcatctac attactgaag cccaggggaa taaatggatg ggcaataccc
    11641 gtagccagat ccaattctaa aacttcattg aaacgatcaa ccataagctt ggctacatct
    11701 ttcggggcag tgatatcctt atccctaaca aaatcctcca tatgcaggtg ggacatgggc
    11761 ataagaacaa aatccaactg ctctgcgacc tctttggtta tcccaacctt accgccgccg
    11821 cagtattccg tctcacagcc gatgaaaacc ttgatgccag agatatcctg cgggagttcc
    11881 tctttaattc tcatgatatg atcaaaatct tggggcttat accattcaga ggcaccagga
    11941 atgttactgt cccaaaaatg gtttgcaaac cctatgagct ttaagcccct gtccctcgcc
    12001 agctttatat agttttcagc cgtggcagcg ggatcgtccg aacaggatga tagatgtgtg
    12061 tgaacgtgta aatcgtgctc tattttcatt accatacccc tttcccttag tgaatatcat
    12121 aattatagca taaatggata caataatgct tctgtatgtt attaaaaagc ttgacaacaa
    12181 caaaataaaa gtaataccct atgataccga aaacccatgc taatcgttta tcgcaaaaca
    12241 tgcgggtatt atccattgga taatatagag ttgattttag tgacaccttt aatatagaat
    12301 aggggtgggc aataagccgg gaccggtttg tcggtaaccg aggctttttg ctgatttgac
    12361 ggatttagcc tgttaataac agcattttta gcagcatttt gaagtttttg aggcacttat
    12421 aagacggggc gaatatgggg ataaccctcc tgggggattc cagcctattt aattccttta
    12481 aaatgaggcg gtggatggaa gtgtattgtg ccgttgaaat ctatgtatgc aatttttgag
    12541 aaatccaatg aaaagggatg ataagatgga caaaattagg ctggcaagac caacaaaacc
    12601 acagatgatg tggcaggatc tggagatggg gatgttttgt catttcggga ttaacacctt
    12661 ttgtgatcag gagtgggggg acggcaccga cgaccctatg gtgtttaacc ctagttcatt
    12721 agacgcccgc caatgggtaa aaaccgccaa gtctgcggga tttaaatatt ttattctcac
    12781 cgccaaacac catgacggat tttgcctctg gcctacgaaa accacggatt attcggttaa
    12841 atcaagccct tggaaggatg gcaggggaga tatagtgaaa gagtgtgcca aggcctgccg
    12901 ggaggaaggg atgctctttg gtctgtacct gtccccctgg gacaggcatg aaccccgata
    12961 tcaggataag gatgcttatg atgatttcta tgctgaacag ttgaccgaac tcttaacagg
    13021 gtatggaccc ttggtagagg tatggttcga cggggcaggt tcggaaggcc gggagtacga
    13081 ctggcccagg atcataggct tggttaaaaa gtatcagccg gaaaccatga tctttaatat
    13141 gggtgccccg accataagat gggtaggtaa tgaagacgga gtggccccat acccctgctg
    13201 gaacacagcc gaagatgcca gaatcagcat gttcaccgat gacaccgtaa aatgggacac
    13261 agatactccc ctttggctgc cggcggaatg cgacgtgccc atcagacgga gacaatggtt
    13321 ttggcacccc gatgccgaag aaaatttact ctccctcgaa gacttaaaga atatatatta
    13381 tcgatccgtt gggcatggaa ccaatctact gctcaacata gccccggatg acaggggcct
    13441 gattccggat tccgacgcta aaagggccat ggaatttggc cgttgggtcc aagaaacctt
    13501 ttcggatccc ataaaagaga tatccggagc aggggatagg ctggagatta ggcttgattc
    13561 cccacagacg atatcccata ttatcctcat ggaagatatt gagcatgggg agagggttag
    13621 agattatgct atagactaca gaaaagatgg cagatgggta gaactggttt gtggtagcgc
    13681 tatagggcac aagaagatcg ataggtttga accaataacc acggaccatg tgcggattag
    13741 gatattggaa acggcagata aacccattat aagaaagttt gccttagtat aattataacg
    13801 aggcggtagg tataaatccc ccgcctcgtt gaaacctgtg tgttgcaatt gccagtgcaa
    13861 ttgcttccga tgttataacg agtcggtaaa atatcccctg catctataag cggcgagttc
    13921 cgattagtaa agtataacag gcgatgggta taaatccctc gacccgttga aacctgtgtg
    13981 t
//
LOCUS       DASRNX010000186        20705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_514097,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000186 DASRNX010000000
VERSION     DASRNX010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 20705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20705
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514097"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..224
                     /gene="rrf"
                     /locus_tag="VFD89_07495"
     rRNA            108..224
                     /gene="rrf"
                     /locus_tag="VFD89_07495"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            436..1104
                     /locus_tag="VFD89_07500"
     CDS             436..1104
                     /locus_tag="VFD89_07500"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HZJ58064.1"
                     /translation="MRGRIFIGILLIALGLGFLLDRLGIILFSTLISTYWPAILILVG
                     ASQLFSKGHSEISGIILIFLGSFFLLKKLELLPEDIGKYFWPALLIIIGIIIIFGRSR
                     HSGMPVSGEDAVNHFVVFSGLENKCISQDFKGGSATAIFGSIDLDLRDANLREEGAFL
                     DLTAVFGGISLRVPDRWKVIATGTPLFGGWENKTRTPSDMPQDQPNLKIRCFALFGGM
                     EIKN"
     gene            complement(1106..2032)
                     /locus_tag="VFD89_07505"
     CDS             complement(1106..2032)
                     /locus_tag="VFD89_07505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZJ58065.1"
                     /translation="MVPHKKLKVVSIIVFFFLGMALGIISARGDRAKSPGDMQYTTPE
                     ADKKQFINFDAHKAHPRNQDDYNNGVAGAIQEDEDNKVNGDLQPDVVYYKKARVLQNK
                     YPDIFIMNSTTEKKRIALTFDDGPENITTPKILDILKENKISATFFVLGREIKRYPDV
                     MERMINEGHQVANHSWSHLRPTSLMDPEFIEEITLAKEILDDYMDLDVPFYYRPPYGL
                     LTPSQIEQMGEKGYMAISWSVDSLDWTGSASEEIQDKVIDSVHPGAIVLMHCAGGKDR
                     RSETTKALPRIIETLQEQGYKFVTIDDLLNNE"
     gene            2329..2658
                     /gene="trxA"
                     /locus_tag="VFD89_07510"
     CDS             2329..2658
                     /gene="trxA"
                     /locus_tag="VFD89_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZJ58066.1"
                     /translation="MSKNIVYLNQQNFKNEVLNNKNPVLIDFYADWCAPCRMITPILE
                     QVAKEYEGKATIAKLDVDANNALASQFGVMSIPTLVFFRGGKEVQRVVGVRPKSELTR
                     TLDKMIG"
     misc_binding    3160..3370
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3417..4688
                     /gene="serS"
                     /locus_tag="VFD89_07515"
     CDS             3417..4688
                     /gene="serS"
                     /locus_tag="VFD89_07515"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZJ58067.1"
                     /translation="MLDPKFIRENIEIVKEGLNKRKEDIDLDKFLLLDQNRREYIAQT
                     EQLKSRRNAVTQDIAKLKRQKKDASHLIKEMQDVSKAIKELDDKVREAQEGLDELLLT
                     IPNIPSPSVPVGETDENNETMREWGQPTTFDFEPKAHWDVGEGLGILDFAKAGKVTGA
                     RFTFYKGLGARLERALINFMLDLHATEHGYTEIFPPFMVHRRSMVGTGQLPKFEEDAF
                     RIANTEYFLIPTAEVPVTNMYREEILDEERLPIKHVAYSACFRAEAGSAGRDTRGLIR
                     QHQFNKVELVKFVKPENSYEELEKLTNDAENVLRLLELPYRVSMLCSGDLGFASAKTY
                     DLEVWMPSYGRYVEISSCSNFEDFQARRADIKYRPARGGRAAFVHTLNGSGIAIGRTV
                     AAILENYQQDDGTVIIPERLIPYMGGIDRIK"
     gene            4882..4970
                     /locus_tag="VFD89_07520"
     tRNA            4882..4970
                     /locus_tag="VFD89_07520"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4916..4918,aa:Ser,seq:tga)
     gene            5076..5166
                     /locus_tag="VFD89_07525"
     tRNA            5076..5166
                     /locus_tag="VFD89_07525"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5110..5112,aa:Ser,seq:gct)
     gene            complement(5230..6087)
                     /locus_tag="VFD89_07530"
     CDS             complement(5230..6087)
                     /locus_tag="VFD89_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HZJ58068.1"
                     /translation="MGNLLVFQSDFGIADGAVSAMYGVAYSVSRDLTICDLTHEIPQY
                     NIWEASYRLIQTVQYWPKGSVFVSVVDPGVGSTRKSVAVRTKGGQYIITPDNGTLTHI
                     KRMSKIIEAREIDEETNRLPYSGKSHTFHGRDIYAYIGARLATGIIEFQDIGQEIDTN
                     DVVELNVPEASKINDIITGAIDIMDVRFGSLWTNISRELFDELGIVYGGRVEIAILND
                     TREVYRNIIRYGKSFADVHIGEPLVYINSLDYIAVAINQGSFSKAYNIHTGSNWIITI
                     RRAPKIIYE"
     gene            complement(6109..7464)
                     /locus_tag="VFD89_07535"
     CDS             complement(6109..7464)
                     /locus_tag="VFD89_07535"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HZJ58069.1"
                     /translation="MKYNFIDYRSDLIRDVSELIKIPTVFHGASATEVMPYGKNVYEG
                     YIWLKEKAQNDGFKVTEFDGHALAIRIPQNRAPERIDVASHVDVVEPGKGWEHEPFGG
                     AITGGYIHGRGAQDMKGPLIATYYALKYIKDNQIPCKRELRIVIGCDEERTMNDMRYY
                     IAKANEPAFAFTPDGRFPYSYGEKGALMWTIDEEIDAPIIELNGGVQCNVVSPVATAL
                     IGGIEAVDAYRSMLFKYGYEGTVIIKDGKTLIGIMGKAAHASRPADGVNATVRLLRLV
                     GEVTGDKISQLLYRCFSDFYGKGANIAYDIDPMGRLTLNLGTLKVADRHLKAEIDCRY
                     PHGIASDILTMRMNKALKPLKAELAYDDKPTLTDRESPFLKVLLSTYREIAHDPTAAP
                     SIGLGVTYSKVFSNCVAFGPLRENDQSLAHQANERIEIKLLCKLMRIYAHAMIRLANM
                     D"
     gene            complement(7461..8315)
                     /locus_tag="VFD89_07540"
     CDS             complement(7461..8315)
                     /locus_tag="VFD89_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HZJ58070.1"
                     /translation="MMKSPLLNLIPGDTYLHKLTGKTKVRSFVILLVYIIMSFDLRLL
                     LPLLVAGIIGLISLRPQWKTLRYFFIIVAAVNLLNILLFWLVNPDIGAYWCGGSRTVL
                     VSFSEKFFISLETLWYLSIRFLKMITSFLISTVFILSVTPSEMAAGLYSIKVPYKICT
                     IFSIAFRYIPDIGRDYESIKISMQARGVELDPKKVSLVDRIKQNVLILIPLIITSFER
                     VGNISNAMDLRGYGRGKTRTYYSEHEERKADRIFKTFYSGLLLFCAYWVVSRLVSPPP
                     SKMWYPFA"
     gene            complement(8293..10011)
                     /locus_tag="VFD89_07545"
     CDS             complement(8293..10011)
                     /locus_tag="VFD89_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58071.1"
                     /translation="MRKPIVEFIDFGFRYKSQSSPTLFNINLAIYPGEKILILGPSGS
                     GKSTLANCINGLNPFFYDGEITGSCKVAGRETKTASIFALSKTVGTVLQDSDAQFVAL
                     SVGEDIAFALENQSVPREEMIPKVQWASEIVGMEDFLLHLPYELSGGQKQKVALAGVI
                     HDDVDLLTFDEPLASLDPQMGMYAIDLIHEIHVDQKKTVIIIEHRLEDVLYRHVDRVI
                     LMNEGRIVFDDTPDKLLTSNLLPKYGIREPLYIMAMKYAGCTFTGHEVLSDIISMDLS
                     PFEEKLKDHYRTEIGRTAIPAKDEILRVRDVSFSYGDGDVLKDFSFSIDRGEKVAFIG
                     KNGAGKSTMAKLLCGIIRPQKGEIHVEDSNYLKFTIKEIGEKIGYVMQNPNQMLVKDI
                     ILDEVELALLLRGKSKTEIDAAVERALQMCGLYGMRNWPIFAVSYGQRKRVTIASILV
                     LQPDIIILDEPTAGQDYYHYTEIMNFLDDLNRQYGITIIFITHDMHLAIEYTDRAIVF
                     SDGKLIADDSVFKVLSNDEVITKANLKQTSLYTLATRLDLDPETYIEHFIAYERMKKD
                     DEKSIT"
     gene            complement(10013..10606)
                     /locus_tag="VFD89_07550"
     CDS             complement(10013..10606)
                     /locus_tag="VFD89_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013739995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HZJ58072.1"
                     /translation="MKSTSKRILGVWNTKTIVAVAIGAALFGVLMNFGGIPVFTNTKL
                     TTAMIIPVIVGAMFGPVPAALACAVGNLIADLIGGWGYWFDWSIGNGVLGFIIGLLPL
                     YGANIDEGIFKPIHGVIYTITCIVGNYLAFGVITPLLTTLFYGGELEVTFLQALAAGL
                     SNTLILVVVGIPVLILLARRNARGTDLKLEEKEEQNI"
     gene            11594..12424
                     /gene="thyA"
                     /locus_tag="VFD89_07555"
     CDS             11594..12424
                     /gene="thyA"
                     /locus_tag="VFD89_07555"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZJ58073.1"
                     /translation="MSRADTIFMKMCRDIIDEGFSSEGQKVRAVWEDGTPAHTIKKFG
                     VVNRYDLSEEFPILTLRGTNLRAAIDELLWIWQKKSNNTRDLSSRIWDSWADESGSIG
                     KAYGYQMGVKHRYAEGEFDQVDRVLYDLKHNPYSRRIMTNIYVHGDLHEMNLYPCAYS
                     MTFNVTGQKLNGILNQRSQDVLVANNWNVCQYAILMHMFARVSNLEVGELVHVIADAH
                     IYDKHIPLVKGLISRESYPAPKLIINPDVGDFYDFKVDDFVLENYQRGPQIKNIPVAI
                     "
     gene            12579..13076
                     /locus_tag="VFD89_07560"
     CDS             12579..13076
                     /locus_tag="VFD89_07560"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZJ58074.1"
                     /translation="MQAILSADLNWGIGYRGGLLMRVPEDMKFFKAMTIGKVVVMGRE
                     TFESLPGKKPLKERINIVLSKTAKYNDEGIVLCRSMEELFKELKKYPPNDIFIIGGES
                     VYTQLLPYCSKVYVTRFEKEFTADRHFPNLDTTENWEMAEESQRHYYKDLSFKFLTYV
                     PGGRG"
     gene            complement(13368..14744)
                     /locus_tag="VFD89_07565"
     CDS             complement(13368..14744)
                     /locus_tag="VFD89_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HZJ58075.1"
                     /translation="METKRNTNIYTFGLGTVGRDMVYAMVTMYLIFYLTDVLGVSSGA
                     LWWINGIILIARVFDALNDPIMGVIVDNTHTRYGKFKPWIAFGALASGILTILLFTDF
                     NLGEKGFVTLFGLIYILWGMAYTTNDISYWSMLPSLSMDQREREKIGAFAKISANIGL
                     FAVVAGIVPITAALGNKLGDMVKAYTIFTVALVLIMWAMQAITLFGVKEKEGLFKQEE
                     RTNLKGMFRAIVDNDQLLYIAIAMSLFMIGYTTTTSFGLYFFKYSYGDENMYSIFAVI
                     LGLSQITALIVFPLFSKAHSRKTLYTVATGLVIVGYIIFFFSPMDMIFIGISGILIFL
                     GQAFIQLLMLMFLADTVEYGQWKLGKRNESVTFSLQPFIYKIGGAISSGIVGATVIIS
                     GINDANTAADVTSGGLLMMKGAMLILPLLFILASYLIYRYRYRIDSDMYGKIISELQD
                     RGDINPIA"
     gene            complement(14863..17103)
                     /locus_tag="VFD89_07570"
     CDS             complement(14863..17103)
                     /locus_tag="VFD89_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZJ58076.1"
                     /translation="MNIYSRYAPFIQDFIYQNNWESLRAIQVAAGDIIFNTDHNLLLS
                     ASTASGKTEAAFFPILTLFREDPPKSIGALYVGPLKALINDQFLRLNDLCREADIPVW
                     HWHGDVAQSHKERLLKKPSGILQITPESLEALLMRRHSYIPRLFSDLRFIVIDEIHSL
                     MRGDRGGQTLCLIQRLSRLAGVNPRRIGLSATIGDLEAAGKFLSGGSGRETAAPKISV
                     KGAKWRLSMEHFFIQSQQAGDDRDDSAAHLMALDEKTDTAPAAADPGIGYIYEHTRDK
                     KCLVFVNSREECEAVTTTLRQYCEYQRTPDRFLIHHGNLSTSFRETAEAIMKDDEEYQ
                     TTVTTATLELGIDIGRLERAFQIDAPYMVSSFLQRMGRTGRRDLPPEMWFVMREDEAE
                     SRAMLPATIPWKLIQGIALVQLYLEERWVEPPNLDRLPYSLLYHQTMSTLASSGEMSP
                     PMLASKVLSLSYFHRVTQDDYKSLLRHLISIDHLQRTERGGLIVGLSGERITNSFKFY
                     AVFKENEEYTVRTKSQELGTIVMPPPAGEKIAIAGHVWIVEEVDHKRRLVYCEKVKGN
                     VPAYFGLCPGDINTKVLEKMRDVLKTGTVYPYLMKNAVSRLGEARYIATKSALTERPL
                     INLGGDMWCLFPWLGTYAFLALERFLKLICAKELGIASVDSSRPYFIQFKMKATEEAF
                     FKTLRELEKSALNPMDLVYPGEIPVFEKYDEFLPEELVKKGFAYGVLGIREMKKRIRS
                     WGNPVY"
     gene            complement(17113..18429)
                     /locus_tag="VFD89_07575"
     CDS             complement(17113..18429)
                     /locus_tag="VFD89_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ58077.1"
                     /translation="MKVPKRVAQVLINSLKGGVVPRIGLPYITVGREAEINALLHDID
                     IVGEGGASFRFIVGKYGSGKSFLLQAIRNYSMDRNFVVVDCDLSTERRLQGTRGQGLA
                     TYKELIRNMSTKTKPEGGALTLILDRWLSNVQTQVMKESNLSFKDPELPLLVEKKIFK
                     VIDDLHEMVHGFDFAKLLGIYYSAYINDQELEKAKVVKWFRGEYTNKTEARAELGVNI
                     VITDDDWYEYIKLFAMFLKKAGYSGLLILVDELVNIYKIPNSISRQYNYEKILTMYND
                     TLQGKAHYMGIVMCGTPQCIEDTRRGVYSYEALRSRLQVGRFGSENLIDFLAPIIRLS
                     PLTGEEMLVLVEKLSKIHSGLFGYESTLTQEDLINFIKIEFGRIGADINITPREVIRD
                     FIELLNIVYQNPAIDVTALLNSDGFQYAKGGLDEGPTERTFEEFEI"
     gene            complement(18426..19865)
                     /locus_tag="VFD89_07580"
     CDS             complement(18426..19865)
                     /locus_tag="VFD89_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HZJ58078.1"
                     /translation="MNDAKKLVSLINNVVYKDEPILTTAADLLKFTPPKYRQMRRIAK
                     ENHTYLETDPAIFYKQGKFMENFVDDYEYTGEFFRYYPTYQSMNDQQLRGYFTWRTRV
                     RQGTIVRTSLSFVFVYIYELINGIGTVTPMDGYTKLKTLWDYYGPIDPKINRYMALWL
                     KDYVIYYNLDRSLLEILPGIGFNDAATVLLDYRSREPQGVFSALNSLSPYNLKNSRFY
                     KSYPDDVQYVVFNVFDSLSEYHIKHRKNSLLEKFFGKVFKSDYGIFYSAIFSDPLKVK
                     DATYEISKAHKFRCRNGKWSEERFFNYKEKNKEIGRLLKTVDFVMREHYGFKHKLKVD
                     KVTKLYSRAILGAVAELVQYNRRQARSNVKIDVSKLGGIRASALATQERLITESEIEE
                     MAPEPGLLANELNPCNLTAQEYKFVRCLICGGDYRDILNSHGIMESVIVDSINEKLFE
                     VFDDIVIDFNGVKYEVFEEYMEELKGIFL"
     gene            20356..20517
                     /locus_tag="VFD89_07585"
     CDS             20356..20517
                     /locus_tag="VFD89_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58079.1"
                     /translation="MAKVTCSICGYPFEQGDITICPDCSAYVCEECSYMYRGHCKDCY
                     QEVTLDFEE"
BASE COUNT         5686 a         4507 c         4669 g         5843 t
ORIGIN      
        1 tagagaagta gagttagtaa tccggagacg tgtgtatgat acagacccta agggcagacc
       61 taaaagcatc cgacttccaa cttctaattt ctaacctcta gcaaatttcc ggtggcgatg
      121 gcgaagggga tacacctgtt cccatcccga acacagcagt taagcccttc agcgccgatg
      181 gtacttgggg ggagaccccc tgggagagta ggtcgctgcc ggattccaat atatcaaagc
      241 cccatgagaa atgactcttg gggctttgct ttatgtcttt atttatattt tattgaaggg
      301 ttatcctagt tggatagctg ctacatcgga aacgatgatc cgccaaccat ggcccccatt
      361 taaaaactta ttgacttgag gtttatattg ctataagctg gaagtggatt aaattgatgc
      421 atggggggag aaaatatgcg aggtcgtatt ttcattggaa ttttgcttat tgcactgggg
      481 ttgggattcc tactggacag actgggtatt atcctattct ctactctgat atctacatat
      541 tggcctgcta tattaattct ggtaggcgca agccaacttt tttccaaggg acattcagaa
      601 atatcgggga ttattctaat cttcctgggt tcgttctttc tccttaaaaa acttgaactg
      661 ttaccagaag atattggtaa atacttttgg cctgctctgt taattattat aggcatcatt
      721 attatttttg gcaggtcaag acattccggt atgcccgtgt ccggggaaga tgcagtaaac
      781 cattttgttg tcttttcggg attggaaaac aaatgcatct cccaagattt caaggggggg
      841 agcgctaccg ctatattcgg aagtatcgat ctggatctcc gggatgcaaa cttacgggaa
      901 gaaggtgcgt ttttagattt aactgctgtg ttcgggggta ttagcctaag ggttcccgac
      961 agatggaagg tgatcgcaac gggaacacca ttatttgggg gatgggagaa taaaacaagg
     1021 acaccaagtg atatgcctca agaccagcca aaccttaaga tcagatgttt tgccctgttt
     1081 ggcggcatgg aaattaaaaa ttagactatt cattatttaa aaggtcatct attgttacaa
     1141 acttatagcc ttgctcctgg agggtttcga taatccgggg cagggctttt gttgtttctg
     1201 atcgtctgtc tttgcctccc gcacaatgca ttaagactat agctccggga tgtacagaat
     1261 ctataacctt gtcttgaatc tcttccgacg cggagccggt ccaatccagc gaatctacgg
     1321 accaactgat ggccatataa cccttctctc ccatctgttc gatttgtgat ggggtcaata
     1381 gtccatatgg agggcgataa taaaatggta catctaaatc catataatca tccaaaatct
     1441 ccttagctag cgtgatctct tcaataaatt ccggatccat gagcgaggtg ggtcgtagat
     1501 gcgaccagct atgatttgca acttgatgcc cttcattaat catcctttcc attacatcgg
     1561 gatagcgttt gatttcccga cctaggacaa aaaaggtagc cgaaatcttg ttttctttta
     1621 gaatgtcaag tattttaggg gtggttatat tttcgggacc gtcatcaaat gttagcgcta
     1681 ttcttttttt ttcggtggta gaattcatta tgaatatatc aggatattta ttttgtaaga
     1741 cccttgcctt cttatagtat actacgtccg gttgtaaatc cccattcact ttgttatctt
     1801 catcttcttg gattgccccg gcaaccccat tgttatagtc atcttgatta cggggatggg
     1861 ccttatgtgc atcaaaattt ataaattgtt ttttatcagc ctcaggagtc gtatattgca
     1921 tatctccggg gcttttcgcc ctatcgcctc gagccgaaat aatccccaat gccataccga
     1981 gaaaaaagaa tactattatg gatactacct ttagtttttt atgcggtacc aactgaagcc
     2041 ctccatttag acaggtgatt cgattgctat cattatagtt tcaccaaacg tataaaaaat
     2101 aacaataaag ttcaaaaata cattatccaa cggtttagat gctgctgata attcagactc
     2161 ttgatacatc ggctgctctg tattttttga tagcgtctaa tggatgctgt aggagtgaag
     2221 ggatcattat ttatagataa aatattttaa aaagtagttg acaaagggct aataacagtg
     2281 tattataata ttacaatata acgattaaat tgtagggagg cctttataat gagtaaaaat
     2341 atcgtttatt taaaccaaca aaattttaaa aatgaagtgt taaacaacaa aaaccctgtc
     2401 ctgattgact tttatgctga ctggtgtgct ccatgtagga tgataacacc gatcctcgag
     2461 caggtggcta aggaatatga gggaaaagct accatagcga aacttgatgt agatgcaaat
     2521 aatgcgttag cttcacaatt tggagtgatg agtatcccca ctttggtctt ctttagaggc
     2581 ggtaaggaag ttcagcgcgt cgttggagta cggcctaaat cagaacttac ccgtacccta
     2641 gataagatga ttggatagaa agcaataccg gctgtctcat ggcagccggt ataaatatat
     2701 ataaaaggag gtcaaaaaaa caacttgttt ccataattgt ttagtcctag ttgggttgtt
     2761 aacaaattat cattgtttct taaatctatt atactcctaa acggataaat ttcaacaact
     2821 ttcgataaat tcagcattat atataaacat aactatccgt ttttagaatt tatgtataga
     2881 aaacctgccc taaccaaatg atttccttaa atggcatctg ttattctaaa attatataac
     2941 gggtcggcaa gatgtctctc gcttccataa acggcgggtt tcgattagta aagttaaact
     3001 ggcggtgggt acaaatcccc cgcctcgttg aaacctgtgt gttgtaattg ccaatgcaat
     3061 tgcttccaat gttttaaaaa attaagaagt taaaacataa ggcgggacta ttgcatatcc
     3121 aaggcaatat agtataatgg tagataacaa ttgaattagt gaagagcaat gaagagaaat
     3181 agtagataag caaaggttct taagagaacc ggtggtaggt gtaaaccggt ggaccttttt
     3241 atcaaatccg tctctgagct atgggccgaa gttatagtaa gctttatcgc cggctgggcg
     3301 ttatccggtt atgaaggtgg aggatatcaa tcctcaacta gggtggcaac gcgggtttct
     3361 cgtccctttt tagggaacgg gatttttttg ttttggtaaa cttttggagg tgtattatgt
     3421 tagatcccaa atttataaga gagaatatag aaatcgtaaa agaaggacta aataagcgaa
     3481 aagaagacat cgatctagat aagtttttgc tgctggatca aaacagaagg gaatatatcg
     3541 cccagactga acagctaaag agcaggagaa atgcagtcac ccaggatata gccaaattaa
     3601 aacggcaaaa aaaggatgca agccatctta tcaaggagat gcaagatgta tctaaagcca
     3661 tcaaggaatt agacgacaag gtaagggaag cccaggaggg gctggatgaa ttattgctga
     3721 caatacccaa tatccccagc ccatctgttc ccgtaggcga aaccgacgag aacaatgaaa
     3781 caatgagaga atgggggcaa cccacaacct ttgactttga acccaaggcc cattgggatg
     3841 taggggaggg tttggggata ctggactttg ccaaggcagg taaggttaca ggagccagat
     3901 ttacctttta caagggcttg ggtgccagat tggaaagggc actgatcaat ttcatgctgg
     3961 accttcatgc cacggaacat gggtatacag agatattccc gccctttatg gtccatagaa
     4021 ggagtatggt cggaaccgga caattgccca agttcgaaga ggatgccttt aggatagcga
     4081 atacggaata cttcttaata cccactgccg aagtgccggt gaccaatatg tacagggaag
     4141 agatactgga tgaagagcgg cttcccataa agcatgtagc ctatagcgcc tgctttcgag
     4201 cagaggcagg ctctgcaggt agagataccc gcggtcttat caggcaacac cagttcaata
     4261 aagtggagct ggtcaagttt gtaaagcccg aaaattcata tgaggaattg gaaaagctga
     4321 ccaatgatgc ggaaaacgta ctcagactct tagagctccc ctatagggta tcgatgctgt
     4381 gtagtgggga tctgggcttc gcctcggcaa aaacttatga tcttgaagtg tggatgccaa
     4441 gctacgggag gtacgtggag atctcctcct gcagtaattt tgaggatttt caggcccgaa
     4501 gagcagatat aaaatatagg cccgcaaggg ggggaagggc agctttcgtt cataccttaa
     4561 acggctcggg gatagccata gggcgtaccg tagcggccat tctggaaaac taccagcaag
     4621 atgacggaac ggttataata cccgaaaggc ttattccata tatgggcggg atcgacagga
     4681 ttaaataaaa catagaaccg atatggcacc gatagaccga ttaggatgaa atggtaacaa
     4741 cgcccacctt tcactaaatt tgaccgcgaa aagctggttg acagggtatt ttacttttgg
     4801 tatacttaga aagtgtcgat tgtgggatct acaagggcat aggtcccgca agacacttta
     4861 gatcttagca gaatccattg tggagaggtg tccgagaggt ttatggagct ggtcttgaaa
     4921 accagtgaca ccgcgaggtg ccgggggttc gaatccctcc ctctccgcca agtagggagt
     4981 tagggattag ggggcaaatg gatagattaa aagaaatagc cgtcccgtcc tttccatagc
     5041 agacccgatt cccgaaacac tccaacatat aatacggaga agtacccaag tggccgaagg
     5101 ggcgcccctg ctaagggcgt aggtcgggtg accggcgcga gggttcaaat ccctccttct
     5161 ccgccagaaa cccgcaatta aggatttaat ccttgtattg cgggtttaat attttctctg
     5221 gaccttttat cattcataga taatttttgg agctcttctg attgttatta tccaattaga
     5281 accggtatgg atgttatagg cctttgaaaa agaaccctgg tttatagcaa cggcaatata
     5341 atccaaagaa ttaatataca ccagaggctc gcctatatgg acgtctgcaa aggattttcc
     5401 gtaccttatg atatttctat agacctcccg ggtatcgttt aggatggcta tttcaaccct
     5461 gcccccatag actatgccca gctcatcaaa gagttccctg cttatattgg tccacaggct
     5521 gccgaagcga acatccatta tatctatggc cccggttatg atatcgttta ttttgcttgc
     5581 ctcggggacg ttcagctcta caacgtcatt tgtatcaatc tcttggccta tatcctgaaa
     5641 ttcgattata cctgtggcca gccttgcacc tatataggcg tagatgtccc tgccatggaa
     5701 ggtatgggac ttgccggaat agggcaatcg atttgtttct tcatcgatct ccctggcttc
     5761 tattatcttc gacatacgtt ttatatgtgt aagggtcccg ttatcagggg tgataatgta
     5821 ttgacccccc ttagtcctaa ccgcaacact ctttcgggtc gaaccgacac cgggatccac
     5881 taccgataca aatactgagc ctttgggcca atattgtacc gtttggatca atctataaga
     5941 ggcttcccaa atgttatatt gggggatttc atgggttagg tcacagatag tcagatcccg
     6001 actgacggaa taggccacgc catacatggc gctgacggcc ccgtcagcta taccgaagtc
     6061 tgattgaaaa accaataagt ttcccatagc tacctcctaa aatgcatatt agtccatatt
     6121 agccagcctg atcattgcat gggcatatat tctcataagc ttacaaagca gtttgatttc
     6181 aattctctca ttagcctgat gagccagtga ttggtcattc tcccttaaag ggccgaaggc
     6241 aacacaattg gaaaagacct tgctgtaggt aacccccaac ccgattgaag gcgcagctgt
     6301 cgggtcatgg gctatctccc tgtaggtgct taggagaacc tttaggaaag gggattccct
     6361 gtccgttagg gtgggcttat cgtcgtatgc taattcggct tttaaaggct ttagggcctt
     6421 gttcatcctc attgttaaga tatcagaggc tatgccgtgg gggtatcgac agtctatctc
     6481 tgcttttagg tgccgatccg ctaccttgag cgtacccaaa ttcaaggtta gcctacccat
     6541 agggtctatg tcataggcta tattggcacc ttttccataa aagtcggaaa aacatcggta
     6601 taaaagctgt gaaatcttat ctccggttac ttctcctact agcctgagaa ggcgaacggt
     6661 tgcattaacc ccgtctgccg gtctggatgc atgggccgct ttacccatta ttccgatcag
     6721 ggtctttcca tccttaataa tgacagtacc ctcgtaaccg tacttaaaaa gcatgcttct
     6781 gtaagcatcg acagcctcta tgcccccgat caaagccgtt gctacagggc tcactacgtt
     6841 acattgtact cccccgttga gctcgatgat cggagcatct atctcctcgt ctattgtcca
     6901 catcagagcc cccttttcgc cataggagta ggggaacctg ccgtcggggg taaaggcgaa
     6961 ggcgggttca tttgcctttg ctatgtagta tctcatatcg ttcatagtgc gctcctcatc
     7021 acagcctatg actatgcgca gctccctttt gcatgggatt tggttatcct ttatatattt
     7081 tagggcatag tacgtcgcaa tgaggggtcc cttcatatcc tgagctcctc ggccatggat
     7141 ataaccgccc gttatagccc cgccaaaggg ttcgtgctcc catcctttcc cgggctccac
     7201 tacgtccaca tgggaagcca catctatcct ttcaggagcc ctgttttgtg gaatacggat
     7261 agccaaggca tgaccgtcaa attccgtgac tttgaatccg tcattttggg ccttctcctt
     7321 taaccatatg tagccttcat aaacattctt accgtagggc atgacttcgg tcgccgaggc
     7381 cccatgaaag acggtcggta tctttataag ctctgagaca tccctaatta aatcactacg
     7441 ataatctata aaattatact tcatgcaaat gggtaccaca ttttagaagg cggtggagat
     7501 acgagtctgg agacaaccca gtatgcgcag aaaagcaata ggcccgagta gaatgtttta
     7561 aagatcctgt cggcctttct ctcttcatgc tcgctgtagt aggttctggt ctttcccctt
     7621 ccatagcccc ttaaatccat agcgtttgaa atattaccaa cgcgttcaaa ggatgtaatt
     7681 attagcggaa tcaatatgag cacgttttgc tttattcgat ccactaagga taccttcttt
     7741 gggtccagtt caactcctct cgcctgcata gaaattttaa tgctctcata atcccttcct
     7801 atatcgggta tatacctaaa ggcaatggaa aaaatcgtgc atatcttgta tgggaccttg
     7861 atggaataaa gccccgccgc catttccgat ggagtgacgg atagtatgaa gaccgtggat
     7921 atcagaaatg atgtaatcat ctttaaaaac cgtatggata gataccaaag ggtttctagg
     7981 gagataaaga atttttcaga aaaactaact aagacggtac ggctgccgcc acaccaatat
     8041 gcacctatat cagggttcac cagccagaac aaaaggatgt ttagaagatt taccgcagct
     8101 acaattataa agaagtatct cagggtcttc cactgtggcc ttagggatat aagtccaatg
     8161 attcccgcaa cgagaagggg cagcaacagt ctaaggtcaa aggacatgat gatgtacacc
     8221 aaaaggatga cgaaggagcg caccttggtt tttcccgtca gcttatgaag atatgtatcg
     8281 ccggggatta aattaagtaa tggacttttc atcatccttt ttcatccttt cataggcaat
     8341 aaaatgttct atataggttt ccggatcgag gtccagccgt gttgccaagg tgtagaggga
     8401 ggtctgtttg aggttggctt tagtgattac ctcatcgttg gataggacct taaataccga
     8461 gtcatcggct atgagtttcc catcggaaaa cactatagca cggtccgtat attctattgc
     8521 aaggtgcata tcgtgggtaa tgaaaataat agtaatccca tattgcctgt ttaaatcatc
     8581 caaaaagttc atgatttccg tataatgata gtagtcttgg ccggccgtcg gttcatccaa
     8641 gatgattata tccggctgta acaccagtat cgatgcaatg gtgactcgtt ttctttggcc
     8701 atagctcacc gcaaatatgg gccaatttct catcccatag aggccgcaca tttggagggc
     8761 tctctctacg gcggcatcta tctcggtttt gctcttgcct cggagcaata atgccagttc
     8821 gacttcatct aaaatgatat ccttaaccaa catttgattg gggttttgca tgacataacc
     8881 gattttttcc cctatttcct tgattgtgaa tttgaggtaa tttgaatcct caacatggat
     8941 ttcgcctttt tgaggtctga ttatcccgca aaggagcttg gccatggtgc tcttgcccgc
     9001 accgtttttc cctatgaagg ccaccttttc ccccctgtct atggaaaagg agaagtcctt
     9061 taatacatcg ccatccccgt atgaaaagga cacatcccgg actcttagga tctcatcttt
     9121 agcagggata gctgtcctcc caatttcggt tctataatgg tccttaagct tctcttcaaa
     9181 gggggataaa tccatactaa taatatcgga cagaacctca tggccggtaa aggtacatcc
     9241 tgcgtatttc atggccatga tatagagggg ctctctaatg ccgtatttgg gcagcaaatt
     9301 ggaggtaagc agtttatcag gggtatcatc aaacacgatt ctaccctcat tcattaggat
     9361 gaccctgtct acatggcgat atagtacatc ctcaaggcga tgctctatga taataacggt
     9421 ttttttctga tccacatgga tttcatgaat aaggtcaatg gcgtacatcc ccatctgggg
     9481 atcaagggat gccaagggct cgtcaaaggt caaaagatcc acgtcatcgt ggattacccc
     9541 cgccaaggcc accttttgtt tttgtcctcc cgacagttca tagggcaggt ggagaaggaa
     9601 gtcttccatt ccgactattt cacttgccca ctgcactttg ggtatcatct cttccctggg
     9661 tacgctttgg ttttccaagg caaaggcgat atcttctccg acgctcaagg cgacaaattg
     9721 ggcatccgaa tcctggagga cggtaccgac ggttttgctc aatgcgaaaa tgctggcagt
     9781 tttggtttcc ctgccagcaa ctttgcatga tcccgttatt tcaccatcgt aaaaaaaagg
     9841 atttaatccg ttgatgcaat tggccaaggt tgacttaccg ctcccgctgg gacccaaaat
     9901 caaaatcttt tcccccggat aaatagctag attgatatta aagagggtgg gggatgattg
     9961 cgatttgtat cggaatccaa aatcgataaa ttctacaatg ggttttctca ttttagatat
    10021 tttgttcttc cttttcttct aatttgaggt ctgtacctct ggcatttcgt cttgccaaga
    10081 ggatgaggac ggggataccc acaacgacca atatcaatgt gttagacagc ccggccgcca
    10141 gcgcctgaag gaaagttacc tccaattctc cgccgtaaaa cagggtggtc agcagtggcg
    10201 taattacgcc aaaggccaga tagttaccta cgatacaggt gattgtatag atgaccccat
    10261 gaatgggttt aaagataccc tcatctatat tcgcaccata taagggcaac agtcctatta
    10321 tgaaacccaa taccccgttg ccgatggacc aatcaaacca atagccccac ccgcctatga
    10381 ggtccgcgat aaggttaccc acggcgcagg ccaaagccgc gggaacaggt ccaaacattg
    10441 ccccaactat aacaggaatg atcatagcag ttgttagttt agtattagtg aacacaggga
    10501 tgccgccaaa gttcatcagg acaccaaata gggccgcacc aatggcaacg gcgacgattg
    10561 ttttggtgtt ccatacacca aggattctct tagaagtgct cttcatcttt tatccctcca
    10621 ttatgtattt tgatgtacac agccggatcg gctgtgaaac attaaaggct aataaatatt
    10681 caccggataa acaacttttt ggtcccgtca aaataattaa gaaatcgtta tcgtctttaa
    10741 tgtaacctag aagatgcaag tttgggtcag gcaattgatg catatgcgaa ttaatgggtt
    10801 atacaatgat gatttgttgg cttttataaa cgatgtcagc gatttatgga taaacaatgc
    10861 gagaaccaaa tctatcggta ttataactgc aatgggtccg tgcaatttca tgatgataaa
    10921 ttttatagtt ggagttgctt atctgtatat gctacagaaa attatgttat aggactcaac
    10981 atacagtctg tgatgtccag tatatatgtt attaatatca ctcatttcta attatatttg
    11041 tatctgagta taaatgcaag caatttatgg gtatgttgga taaattccgg ggatttccta
    11101 tcataagaga ctatatcatt tgtaattgga atggggtgac ttaaattagg tgggactaca
    11161 taaatccaat ggaaagatag gataaaacca agcctacagg ctaaagactg agatgtgatg
    11221 gaaatttcat tccgatccat gtataataca attgggattt cactttaacg gctatatctg
    11281 attagggtta tcaatatagg cagtacaatc aaaggattca ggtgtcaaac attcataata
    11341 tgattttgta ccatcatata ctgtctgacc cgtcggtgtt ataatgttat ataacgaggc
    11401 ggtaagatgt ccccccgctt ctataagcgg cggattctga ttagtaaaac aggcggtggg
    11461 tacaaatcac ccgcctcgtt gaaacctgtg tgttgcaatt gcttccgatg ttttagtact
    11521 gattgttcac gaaatggggg tttggcaccc taatcctcat actaattatt tgtgaaaggt
    11581 tgatggggta tacatgagca gagcagatac catttttatg aaaatgtgca gagatattat
    11641 agatgagggt ttttcatcag aagggcagaa ggttagggcc gtttgggagg atggaacccc
    11701 ggcccatacc attaaaaagt ttggtgtggt aaatagatac gatctttccg aggaatttcc
    11761 catactgacc ctgcggggga ccaatctaag ggcggcaatc gatgagttgc tatggatatg
    11821 gcagaaaaaa tccaacaata caagggattt aagcagccgt atatgggaca gctgggccga
    11881 cgaatcaggt tctatcggta aggcctatgg atatcagatg ggggttaagc ataggtatgc
    11941 agagggcgaa tttgatcagg tggacagggt gctctacgat ttaaagcaca atccctacag
    12001 ccgacgtatt atgacaaaca tatatgtaca tggggatctc catgagatga acctctaccc
    12061 ctgcgcatac agtatgacct tcaatgttac cggtcagaag ctaaatggga tactaaatca
    12121 acgttcccag gacgtgctgg tggcaaacaa ctggaatgtg tgtcaatatg ccatactcat
    12181 gcatatgttt gccagagtta gcaacttaga ggtcggtgaa ctggtccacg tcattgcgga
    12241 tgctcatatc tacgacaagc acatacccct agtcaaaggg ctcataagcc gtgaatccta
    12301 tcccgctccc aagctcatca taaatccgga tgtaggggat ttctatgatt ttaaggtgga
    12361 tgatttcgtc ctggaaaatt atcaaagggg gccccagata aaaaacattc ccgtggccat
    12421 ataaaggcgg gaatttgtaa gaggtctata aatttaaaag ggcgataacc ggcatgaagc
    12481 atgccaaagg gtgatgcgtt tccagggtga aatcatacga cggattctca ccttggcata
    12541 tgggcacttc ataatagatt ctggaaggtg aaacgattat gcaagctatt ttatcggcag
    12601 atttaaactg ggggatagga tacagagggg ggctccttat gcgggtccct gaggatatga
    12661 agttttttaa agctatgacc attggaaagg tcgtggtcat gggcagggaa acctttgagt
    12721 cacttcccgg gaaaaagccc ctcaaggaaa ggataaatat tgtcctcagt aaaactgcca
    12781 aatataacga cgaaggaatt gtcctatgcc gctccatgga agaactattt aaagagctga
    12841 aaaaatatcc tccgaatgat atctttatca taggggggga atccgtatac acccagcttt
    12901 tgccctactg ttcaaaggta tatgtcacca gatttgaaaa agaatttaca gccgatcgac
    12961 actttcctaa tctggacaca acggaaaatt gggagatggc ggaggagagt caacgccatt
    13021 attacaagga tcttagcttt aaatttctca catatgtgcc agggggacgg ggttagtgac
    13081 acatgtgcca atgtgatgcc aaggggacgg gggtcagtga catagggccg gggatattac
    13141 cgccatgtga ccaggatagt cttagaaaat atgtcatgtg atgtattaag caataataac
    13201 gttaatttca tttttttagg ctttggatta ccgattatct atagacaaac aactacaact
    13261 gtagaatata tcggcatgaa attacacaga cccattgcaa ttgtcatacc gataaattta
    13321 gttgacctat tgcttatctg taattagtgg tgactagggg ggcgcgacta agcgatgggg
    13381 ttaatatcgc ctctgtcttg cagctccgat attattttcc catacatatc actgtcaatt
    13441 ctatatctat accgatatat taaatagctg gccagtataa aaagcagcgg caggatgagc
    13501 atggcgcctt tcatcatgag gaggccccca ctggtgacat ccgctgccgt attggcatcg
    13561 tttatgcctg agatgataac ggtggcccct actatgccgc tggatattgc tccgcctatt
    13621 ttatatataa agggctggag ggagaaggta acgctctcgt tgcgtttgcc cagcttccat
    13681 tggccgtact ccaccgtatc ggctaaaaac atcagcatga gtagttggat aaaggcctgg
    13741 cctaaaaata tcagtatccc tgagatccct atgaagatca tatccatggg ggagaaaaag
    13801 aaaattatat agcctactat aacaagaccc gtagcaaccg tataaagggt tttgcggcta
    13861 tgggccttac tgaatagggg aaatactatt agcgccgtta tctgggacag acctaagatg
    13921 acagcgaaga tggaatacat attctcatcg ccataggaat acttaaaaaa gtatagtcca
    13981 aagctggtgg tcgtggtata ccctatcata aatagggaca tcgcaatggc aatgtagaga
    14041 agctgatcgt tgtctactat ggctctaaac atgcctttta agttggttcg ctcttcctgt
    14101 ttaaaaagac cttccttttc cttgacccca aagagggtta tagcttgcat cgcccacatg
    14161 ataagcacca gcgctaccgt aaatatggta taagccttta ccatatcacc taatttgttg
    14221 cccagagcag cggtgatagg tactataccg gccaccaccg caaagagccc gatattggca
    14281 gaaatcttgg cgaaggcgcc tatcttctcc ctttcccttt gatccatact caaggaaggc
    14341 agcatggacc aataggatat atcgttggtg gtataggcca tgccccacag gatgtagatg
    14401 agtccgaaga gggtaacaaa gcccttttcc cctagattaa agtcggtaaa tagaagtatg
    14461 gttaagatcc ctgaagctaa ggctccaaaa gctatccaag gcttaaactt cccatatctc
    14521 gtatgggtat tgtctactat tacccccatt ataggatcat ttagggcatc aaaaactctg
    14581 gctataagaa taatcccgtt tatccaccac agagccccgc tggatacccc cagcacatca
    14641 gtaagataga agatgagata cattgttacc atggcataga ccatgtcccg gccaacggtt
    14701 ccaaggccaa aggtgtagat attggtattc cttttggttt ccataggaga cctcccttaa
    14761 attaaagcat aattaagttc aatttataca tagcagagaa cgcactgttt atggcatgaa
    14821 atcagataat ctaattgatt ttataaccca tatagacaag ccctagtata cggggttacc
    14881 ccagctgcga atacgctttt tcatctcccg aatgcctaaa acgccatagg caaagccctt
    14941 tttaaccagt tcctccggca gaaattcatc atatttttcg aacacaggga tttcccccgg
    15001 atatacaaga tccattgggt tgagggctga cttttccaac tccctcagtg tcttaaagaa
    15061 cgcctcttcc gttgccttca tcttaaactg aataaagtag gggcgtgatg agtctacaga
    15121 tgcgataccc agttcctttg cgcagatcag ttttaaaaat cgttccaggg cgaggaatgc
    15181 ataggtaccc agccagggga ataggcacca catatcccct cctaggttga ttaagggtct
    15241 ttctgtaagt gccgacttgg tggctatata ccgcgcctca cccaaccggc tgacagcgtt
    15301 tttcataaga tagggataga cggtccccgt ttttaagaca tccctcatct tttccaggac
    15361 ctttgtattg atgtccccgg ggcatagccc gaaataggcg ggaacattgc cctttacctt
    15421 ctcgcaatag accaagcggc gtttgtggtc cacctcttcc actatccaaa catggcctgc
    15481 aatcgcaatc ttctcacccg ccgggggcgg cataacgatg gtgcccagct cctgggattt
    15541 ggtacggacg gtatattctt cgttctcctt aaagaccgca tagaatttaa atgaattggt
    15601 tattctttcc ccggaaagcc cgacaatcag cccccctctt tctgtccttt gaagatgatc
    15661 gatggagatt aggtgcctta aaagggattt gtagtcatcc tgggtaaccc gatgaaaata
    15721 gctgagggaa agaaccttag atgcaagcat gggggggctc atttcaccgg aggatgccag
    15781 ggtactcatg gtctgatggt aaaggaggct gtagggcagt ctgtcaaggt ttggcggctc
    15841 tacccagcgc tcctctaaat agagttgtac gagggcaatg ccttggatca gcttccaggg
    15901 gatggtagcg ggaagcatcg ccctggactc ggcctcatct tctcgcataa caaaccacat
    15961 ctccgggggc aagtctctgc gtccggtcct tcccatcctc tgaaggaatg atgaaaccat
    16021 gtagggagca tcgatttgaa aggccctctc cagcctcccg atatcgatac ccagctctaa
    16081 ggtcgcagtg gtgacggtgg tttgatattc ttcgtcatcc ttcattatgg cctctgcggt
    16141 ttcgcgaaag gatgtagaca ggttgccatg atggatcaaa aaccggtcag gggtgcgctg
    16201 atattcgcag tattgcctga gggttgtggt aaccgcctca cactcctccc tggaatttac
    16261 aaagaccagg cattttttat ccctggtgtg ctcatatatg tagccaatgc cgggatcggc
    16321 cgctgccggc gctgtatccg tcttttcatc cagagccatg agatgggctg ccgagtcatc
    16381 cctgtcatct ccggcctgct gactttggat aaaaaaatgt tccatggaga gccgccactt
    16441 tgctcccttg acagagattt taggggcagc ggtctctctt ccggatcctc ccgataggaa
    16501 tttccctgca gcctctaaat cgccgatggt tgcagaaaga cctattcttc gggggtttac
    16561 accggccagc ctcgataaac gctggattag gcatagggtt tgccctcccc tatcccctcg
    16621 catcagggag tgaatctcgt ctattacaat aaagcgcaga tcagaaaaga gccttgggat
    16681 gtaggagtgc ctgcgcatca acagggcctc taaggattcc ggggtaatct ggaggatacc
    16741 cgatggtttt ttcagcaatc tttccttatg ggactgggct acatccccgt gccaatgcca
    16801 gacgggaata tcggcctccc tgcaaaggtc attgagacgc aaaaattggt cgtttattag
    16861 ggcctttaag ggaccgacat acaaggcgcc tatagatttt ggcggatctt cccgaaacag
    16921 ggttaggatg gggaagaagg ctgcctccgt tttgcccgag gcggtagatg cggataataa
    16981 tagattatga tctgtgttaa atattatgtc ccctgcggct acctgaattg cccgcaggga
    17041 ttcccagttg ttttggtaga taaaatcctg aataaagggt gcatagcggc tgtatatatt
    17101 catacttccc ccttaaattt caaactcttc aaaggttctt tccgtgggtc cctcatccaa
    17161 tccaccctta gcatattgga acccatccga attaagaagg gcggtgacgt cgatggccgg
    17221 gttttgatag actatgttca aaagctcaat aaaatcccga atgacctccc tgggggtaat
    17281 gtttatatcc gcacctattc ggccgaattc gattttgata aagttaataa ggtcctcctg
    17341 ggttagggtg ctttcatacc caaagagccc gctatggatt tttgacagct tttctacgag
    17401 gaccagcatc tcttcgccgg taaggggaga aagtcttata atgggtgcta aaaagtcgat
    17461 taaattttca ctaccgaatc gacccacctg gagtcgggat cttaaggctt catagctgta
    17521 tacacccctt cgggtatcct cgatgcactg cggggttccg cacataacta ttcccatata
    17581 gtgggccttg ccctgaaggg tatcgttgta catggtgaga atcttttcat agttgtattg
    17641 cctggaaatg ctgttgggga tcttatagat attgacaagc tcatcaacca ggattaagag
    17701 cccgctatat ccggcctttt tcaaaaacat tgcaaagagc tttatgtatt cataccaatc
    17761 atcatcggta atgactatat tgaccccaag ttcagccctt gcctcggtct tatttgtgta
    17821 ttccccccga aaccatttta caaccttggc tttttccagc tcctgatcgt ttatgtaggc
    17881 cgaatagtag atgccaagga gctttgcaaa atcgaaaccg tggaccattt catgaagatc
    17941 gtctatgact ttaaatatct ttttttccac aagaagagga agctccggat ctttaaagga
    18001 aagattgctc tccttcatta cctgggtctg gacattgctg agccatcggt ccaaaataag
    18061 ggtcagggcc ccgccctcgg gctttgtctt tgtggacata ttccggataa gctccttata
    18121 tgtggccaag ccttgtcccc ttgtcccctg taatcgcctt tccgtggaga ggtcacaatc
    18181 taccactaca aaattcctat ccatggaata gtttcggatt gcctgaagta aaaagctttt
    18241 accgctcccg tatttgccca ctataaagcg aaaggatgct ccgccttccc ccacgatgtc
    18301 tatatcgtga aggagagcat ttatctcggc ctcgcggccg actgttatgt agggaaggcc
    18361 gatccttggg acaacgcccc cctttaatga gttgattagg acctgggcta ctctctttgg
    18421 taccttcata gaaatattcc tttcaattct tccatatatt cttcaaagac ctcatatttc
    18481 acaccattaa agtcgattac gatgtcatca aaaacttcga atagcttttc gttgatgcta
    18541 tctactatga cggattccat tatcccgtgg ctattgagga tatcccggta atccccgccg
    18601 catataaggc atctaacaaa tttatactct tgggcagtca ggttacaggg attcaattcg
    18661 tttgctaaga gtccaggctc cggggccatc tcttcaatct cactctctgt gatgagcctt
    18721 tcctgggtcg ccaaggcgga ggcccttata cctccgagct tggagacatc gatttttaca
    18781 ttggagcgtg cctgccttct gttatactgt actagctctg ctacggcacc taggatagcg
    18841 cgggaatata gcttggttac cttatcgacc tttaatttat gcttaaaacc ataatgttct
    18901 ctcataacaa aatcaacggt ctttaaaagt ctgccaatct ccttgttctt ttccttgtaa
    18961 ttgaagaacc tctcctcact ccacttacca tttcggcaac gaaacttgtg cgccttgctt
    19021 atctcatagg ttgcgtcctt gacctttagg ggatcagaaa atattgccga gtaaaagatt
    19081 ccatagtcgc ttttaaatac ttttccaaag aacttttcta aaaggctatt cttcctatgt
    19141 ttaatatgat actcggataa gctgtcaaat acattaaaga ccacgtattg gacatcatcc
    19201 gggtatgact tataaaacct ggagtttttc aagttgtagg gggaaaggga gtttagcgca
    19261 ctaaaaaccc cttggggctc acgggatcta tagtccaata aaaccgttgc ggcatcattg
    19321 aaaccgatcc cagggaggat ttctaaaagg gacctgtcta ggttatagta gattacataa
    19381 tcttttagcc atagggccat ataccggttt atcttagggt ctatgggacc ataatagtcc
    19441 cataatgttt ttaatttggt atacccgtcc atgggggtga cagttccgat gccatttata
    19501 agttcatata tgtatacaaa aacaaaggag agagatgttc ttacaatagt tccctgtctg
    19561 acccttgtgc gccaggtaaa atagccccgc aattgttggt cgttcatgga ttgatatgtt
    19621 gggtaatacc taaagaattc gccggtatat tcataatcat ccacaaagtt ctccatgaac
    19681 tttccctgtt tatagaagat ggcagggtcc gtttccaggt atgtatgatt ttccttggca
    19741 attcgccgca tttggcggta cttaggaggt gtaaatttta atagatccgc agccgtcgta
    19801 aggatgggtt catccttgta aactacgttg tttataaggc ttacaagctt ttttgcatcg
    19861 ttcaaaatat caccgcctta gggctgaaag cataatgggc cccagataag ggaagatccg
    19921 ataatgtttt agatggtacc gatatatcct tggaagattt tatttaataa tgggctctgc
    19981 ccatggggcg atactctcta aaacatttca cgctctaggg attgatactc tgttaattgt
    20041 accccaaatt gtcataaaaa tcaactaaaa tcgaatgtat gttcggtcgg tttttcaccc
    20101 atcgcccatc atttgagggc acgatcggcg ggggaggctc ctatccccgc cgtacggtaa
    20161 ataaacaatg aaaggttcat ggattcagcc ccttaagcgg tgggtataaa tctccagcct
    20221 cgttgaaacc tgtgtgttgc tattgcccat gcaattgctt ccgatgtttt aattgtaggc
    20281 ccaacataat ttgaataaat tatcgcggcc tagatataat aatggaagta acacatggga
    20341 aatgagggaa cgtctatggc taaagtaact tgctctatat gcgggtatcc ctttgagcaa
    20401 ggggatataa ccatatgccc ggactgtagc gcatatgtct gtgaggaatg tagctatatg
    20461 taccgcggac attgcaagga ttgctatcag gaagtaactt tggattttga agaataaagt
    20521 cttcaaaaag tattggattt tttgtcgaat cagtgctata atctcaagtt ttgaagttaa
    20581 atcgttaaaa ggccagcaac catcatgctt agtggatttt gcctacctgt ggtatctaag
    20641 tatattttag gaaagtttaa aataattttt aaattattaa taccgtagta agcgtcgaat
    20701 ttgca
//
LOCUS       DASRNX010000187         3229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_722841,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000187 DASRNX010000000
VERSION     DASRNX010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722841"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VFD89_07590"
     CDS             complement(<1..1452)
                     /locus_tag="VFD89_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58080.1"
                     /translation="MKRIITILLAIIMLVISVPVTLAAGDQLVIDNKNIYEGMNKSYS
                     KGYKPTVKKGKATIILPLIYEGTNKIVGDRITVTPDLGNPADSPFSYSNYQMDVLLSN
                     NAVNDGKSTASSFLVRLEIPLAANRINGTYPVVINTGYDLKLGEQDKQKTADGSDKSE
                     GQDQPQVTDDKVEQSFTVYVTITDGKDPNAQEPIPEPEPEPEPRPEPKIIIDQYELDP
                     EIVAGKSFDVLVRLKNTEKKWSAHNIKVTYKGETEDVLPGGKTNTFFIDEIPKGKTHE
                     LTLEMKTRQDADPRPQKILLTIEYEDSARSSYSVNEEILLEIRQPLRLEMDEISIPSA
                     VNAGDSLPITTNIFNMGRSTLYNVRCTLEMPGVIPDGSAYLGNMEPGTSGSAEIYAFF
                     GTLDMGGENKSEDNSQSEDDQDDEDSNKNTSRDDGVGKYGRSEGMMTITYEDEYGDVH
                     TKIAELSTNIERPVFDSSRDQGEEPEEKPDRASQ"
     gene            complement(1449..2156)
                     /locus_tag="VFD89_07595"
     CDS             complement(1449..2156)
                     /locus_tag="VFD89_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58081.1"
                     /translation="MENKKVEPIILARNLTKIYKTQKTRLRALASVDMDVLPGEFVAI
                     VGTSGSGKSTLLSLLAGLESPSAGRIVIKGRGIHRMSEEQLVSFRLEHIGFIFQSFNL
                     FEVLTAQENVAFPLMAQGVGREERMEKAKELLTIFELQKHLYHKPGELSGGQQQRLSI
                     ARAIISDPDIVFADEPTGNLDSHNADQIIEILRDICINRGTTILMVTHDMERAKHADR
                     IVYIEDGRIVDREDKAL"
     gene            complement(2156..2812)
                     /locus_tag="VFD89_07600"
     CDS             complement(2156..2812)
                     /locus_tag="VFD89_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZJ58082.1"
                     /translation="MLDEKKDIAISVDHVYKSFGEEEVLHDVNHKFEEGKIHGIVGNN
                     GSGKTVLFKCICGFLHPTKGKIMVDYQQVGKDVDFPEDVGIIIETPGFLPYATGMKNL
                     QILASLNRKVNNRQIGEIIRRVGLDPKMKKPVSKYSLGMRQRLGIAQAIMEDPRILIL
                     DEPFNGLDKHGVSDMRRLIKELKGRGKTILLASHNQADIEELCDTVCEMDSGILTVVR
                     "
     gene            complement(2812..>3229)
                     /locus_tag="VFD89_07605"
     CDS             complement(2812..>3229)
                     /locus_tag="VFD89_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58083.1"
                     /translation="VKTMEMSTPYECMLTIFLLMLLYTLLCVLIMFVMNLKKGNFWGM
                     GSVFIFSLYGFLLNPQLFKEALKLNDEQMYIANVATGWASPLKHATYHMHNFGYDLLP
                     RLWQTYLIFGILIFISFLIAFFSMRKYNFNFAGGPN"
BASE COUNT          783 a          796 c          652 g          998 t
ORIGIN      
        1 ctgggatgct ctgtccggct tttcctcagg ctcttcaccc tgatcacggc tactatcaaa
       61 gaccggcctt tctatattgg tagatagctc tgctattttg gtatggacat ctccatattc
      121 atcctcatag gttatggtca tcataccttc agatctgccg tatttgccta ctccatcatc
      181 tctagatgtg tttttattgg aatcttcatc gtcctgatca tcttcacttt gggaattatc
      241 ctcggattta ttctcccctc ccatatccaa ggtcccaaag aaagcataga tctctgcgct
      301 gccggaggtc ccgggctcca tattgccaag ataagcgctg ccatcgggga ttacaccggg
      361 catctccagt gtgcatcgaa cattgtagag ggtactccta cccatattga atatatttgt
      421 ggtgatgggc aagctatccc ctgcattgac ggcagaggga atgctaattt catccatctc
      481 taatctaagg ggctgcctta tctccaataa tatctcttca ttcaccgaat aggagctgcg
      541 ggcgctatcc tcatattcta tggtcaataa gattttctga ggtctgggat cggcatcttg
      601 cctggttttc atctccaggg tcagctcatg ggttttgcct ttggggatct cgtctataaa
      661 aaaggtattg gtcttaccac cgggcaagac atcttctgtt tcgcccttgt aagttacctt
      721 tatattatgt gcgctccact tcttttccgt attctttaat cttaccaaga catcaaagga
      781 ttttcctgcg acaatttcgg gatctaactc atattggtct atgatgattt taggctccgg
      841 tcttggttca ggctcgggtt ccggttccgg aataggctcc tgtgcatttg gatccttacc
      901 atcggtgatg gttacataga cggtaaagga ttgttccact ttatcatcgg ttacctgggg
      961 ctgatcttgg ccttctgact tatccgatcc atccgctgtt ttctgcttat cctgctctcc
     1021 aagctttagg tcatacccag tatttattac aacgggatag gtcccattta ttctattggc
     1081 tgccagggga atctcaaggc gcaccagaaa gctagatgcc gtagatttcc catcgtttac
     1141 ggcgttattg gaaagtaaaa catccatctg gtaattggaa taggaaaagg gagaatcggc
     1201 agggtttcca aggtccggtg taacggttat cctatctcca acaatcttat tggttccctc
     1261 atagataagg ggcaatataa tggtcgcctt gcccttcttt actgtgggtt tatatccctt
     1321 tgaatatgac ttattcatcc cctcataaat attcttgttg tcgattacca actggtcccc
     1381 cgctgccaag gtaacgggga cagatataac cagcataatg atagctaata atatagtgat
     1441 aattctcttc atagcgcctt gtcctcccta tcgactattc gtccgtcctc tatgtaaaca
     1501 atacgatctg catgttttgc tctttccata tcgtgggtta ccattagaat ggtagtccct
     1561 ctatttatgc atatatcccg taatatttct attatctgat ccgcattatg agaatccaga
     1621 ttccctgtag gttcatctgc aaaaacgata tccggatcgc tgattatagc ccttgctatg
     1681 gagagccttt gctgttgacc cccggatagc tctcccggct tgtgatatag gtgcttttgc
     1741 agctcaaata tcgtcaataa ttcctttgcc ttctccatcc tctcttccct tcccacccct
     1801 tgggccatta gaggaaatgc tacattctct tgggctgtca atacttcaaa gagattaaag
     1861 gattgaaaga taaaccctat atgctctaat ctaaaactaa ccagctgttc ttcgctcatc
     1921 cggtgtattc cccggccctt tataacaatc cgacctgcac tgggggattc taagccggcc
     1981 aacagtgaaa gcagggttga tttcccgctc cccgatgtcc ctacgatggc aacaaattcg
     2041 ccgggcagga catccatatc caccgaggcc aaggccctta atcgagtttt ttgggtctta
     2101 taaattttgg tcagatttcg ggctaaaatg attggttcaa cctttttgtt ctccattacc
     2161 gcaccaccgt caatatgccg ctatccatct cacaaaccgt atcgcaaagc tcctcgatat
     2221 cggcttggtt gtgacttgcc agcagtatag ttttacctcg gcctttaagc tcctttatga
     2281 gccggcgcat atctgagacc ccgtgtttat caagcccgtt aaagggttcg tctaggataa
     2341 gtattcttgg atcctccatg atggcttgag caatgcccag tcgctggcgc attcccagag
     2401 agtattttga taccggcttt ttcatcttgg gatctaagcc cacccgccga attatctccc
     2461 caatctgcct attatttacc ttgcggttaa gggaggccaa tatctgtaga tttttcatgc
     2521 ccgttgcata gggcaagaag ccgggggttt cgatgatgat ccccacatcc tcgggaaaat
     2581 ccacatcctt gcccacctgt tgataatcca ccattatttt ccccttggtg ggatggagaa
     2641 agccgcatat acatttaaac aatacggtct taccactgcc attgtttcct actatgccat
     2701 gaatcttgcc ctcttcaaat ttatgattca cgtcatggag cacttcctcc tcaccaaagg
     2761 atttatatac atggtctacg ctgattgcta tatccttctt ttcatctagc attaattggg
     2821 cccccctgcg aagttaaaat tgtactttct catggaaaaa aatgcgatta agaaacttat
     2881 aaagattaaa ataccgaaga tcaaataggt ttgccataag cgtggcagaa ggtcgtagcc
     2941 aaagttgtgc atatggtagg ttgcatgctt gaggggggat gcccaacctg tcgccacgtt
     3001 ggctatatac atttgctcat catttagctt taacgcttcc ttaaatagct gggggtttag
     3061 taaaaagcca tacagactaa agataaagac acttcccata ccccaaaaat tccccttctt
     3121 aagattcata acaaacataa tgagaacgca tagcagtgta tatagtagca taagcaagaa
     3181 gatggtcaac atacactcat agggggtgga catctccatg gtcttgacg
//
LOCUS       DASRNX010000188         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_621362,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000188 DASRNX010000000
VERSION     DASRNX010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621362"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFD89_07610"
     CDS             complement(<1..429)
                     /locus_tag="VFD89_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZJ58084.1"
                     /translation="MISAGDFRKGITIDIDGQVYTIVDFQHVKPGKGAAFVRTKIKNA
                     VTGSVLERTFSPSERVPRAHVETKEMQYLYNDGNLHYFMDIETYEQIPLNHGQVEDAI
                     TFIKENMNVTIKFFKGEAFSVEPPFFVELEVTETEPGFKGD"
     gene            complement(484..930)
                     /gene="aroQ"
                     /locus_tag="VFD89_07615"
     CDS             complement(484..930)
                     /gene="aroQ"
                     /locus_tag="VFD89_07615"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019532713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HZJ58085.1"
                     /translation="MKILILNGPNLNLLGTREPAIYGKTDLNTLVKGLEKQAQTLGVE
                     VYHFQSNSEGSLIDQIHGAVDKANGIVFNPGALSHYSYALRDAIASVGIPVIEVHISN
                     ISARETFRNLSVIAPVCLGQISGLGMDGYGYALDYLVKHLAKPQLA"
     gene            complement(1002..1532)
                     /locus_tag="VFD89_07620"
     CDS             complement(1002..1532)
                     /locus_tag="VFD89_07620"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HZJ58086.1"
                     /translation="MAPKMPNIILVGFMGAGKSTIGRALAKRLSLTFWDSDCRIEEIA
                     GDSIPDIFKTHGEEYFRGLEYKVLCDLAELQGAIISTGGGAVTNGGLLDLLLSKGLVI
                     HLEASKETIWRRLKGGDDRPMLHHGDLWERINELYRIRYPKYMRAHYTVKTDGKSVAL
                     VVKEIVGLIDGEIASK"
     gene            complement(1522..1821)
                     /locus_tag="VFD89_07625"
     CDS             complement(1522..1821)
                     /locus_tag="VFD89_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HZJ58087.1"
                     /translation="MTQYNEEPIFLKNYVEELVTNMLDELSVRGDFCTCRRCRLDMIA
                     YALNRIPAKYVVTQTGEVYTKISHLEQQLEVDITVALTQAIETVSKNPRHDSHGS"
     gene            complement(2047..>2622)
                     /locus_tag="VFD89_07630"
     CDS             complement(2047..>2622)
                     /locus_tag="VFD89_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZJ58088.1"
                     /translation="DPDKMPDRDLKMEILVKVLNKEIPLKAHAHRADDILTALRIRNE
                     FDLDMTLEHCTEGHRISDFLLEENAKVIVGPLFSDRSKVELKNMTFKAPGVLSKAGVK
                     VAMMTDHPVIPIQYLPVCAAIAVKEGMDEMEALKSITINAAEITGIDDRVGSLEPGKD
                     ADIAIFDGHPLDFRTKAVWVFIDGYAVKRPE"
BASE COUNT          677 a          659 c          490 g          796 t
ORIGIN      
        1 atccccttta aagccgggtt cggtttctgt gacttccagc tctacaaaaa atggcggttc
       61 tactgaaaat gcttcaccct taaagaattt tatagttaca ttcatatttt ctttaataaa
      121 tgtaatggca tcttcgacct gtccgtgatt tagtgggatt tgctcgtatg tctcaatgtc
      181 cataaaatag tgcagattgc catcgttata taaatattgc atctccttgg tctctacatg
      241 ggccctcgga accctttcac tggggctgaa ggtcctctct aaaacgctac cggttacggc
      301 gttcttaatt ttcgtcctca caaaggcagc gcctttaccg ggtttcacgt gctgaaaatc
      361 tacaattgta taaacctgtc catctatatc gatggtaata ccttttctga aatcacctgc
      421 tgaaatcatt gttcccatct cccatccata ataaaaatat agggtcccta tccccaatta
      481 cacctatgcc agttgcggtt tagccaaatg tttcactaaa taatccaacg catagccata
      541 accgtccatt ccgagcccgc taatttgtcc caagcatacg ggggcgataa cggataaatt
      601 acggaaagtt tccctggcac ttatatttga aatatgtacc tcgataaccg gaatgccaac
      661 tgaagctatt gcgtctctaa gggcataact ataatgactt aatgcccccg ggttaaaaac
      721 aatgccattg gctttatcca cggcaccatg gatttggtct atcaaagaac cctcgctgtt
      781 ggattgaaag tgatatactt cgactcccaa ggtctgtgcc tgtttttcca agcctttaac
      841 cagggtattg aggtccgtct tcccatatat agcgggctct ctggtgccca ataggtttaa
      901 attgggaccg tttagtataa ggattttcat gaaaccaaat gccccctaac atttcatgta
      961 catattatca caaaatgatc tgtcttaata gtaaaagatg atcattttga ggctatttcg
     1021 ccgtctatca gccccacgat ttctttcacg accagagcca cactcttccc atccgtctta
     1081 acggtatagt gggccctcat atacttagga tatctaatcc tataaagttc atttattcgc
     1141 tcccatagat ccccgtggtg tagcataggc cgatcatccc caccctttag ccttcgccat
     1201 atagtctctt tggatgcctc taagtggatt accagccctt tcgacaacaa aaggtcaagg
     1261 agtcccccgt ttgtaacagc tccgccgccg gtagatatta tcgctccttg cagttcggcc
     1321 aaatcacata ataccttata ttccagccct cgaaaatatt cttcaccatg ggttttgaaa
     1381 atatccggga tagagtctcc ggctatctct tcaattctac aatccgaatc ccaaaaggtt
     1441 agggacaacc ttttggctag agcccttcca atggtggatt taccggcgcc cataaatcca
     1501 accaggataa tattgggcat tttaggagcc atgggaatca tgcctcggat tcttgctgac
     1561 agtttcaatt gcctgagtaa gggccacggt tatatcgacc tccaactgtt gttccaggtg
     1621 ggatatcttt gtatatactt cgcctgtctg ggtaaccaca tatttagcgg ggatacgatt
     1681 aagagcataa gctatcatgt ccaaacgaca tctccggcag gtgcagaaat caccccgcac
     1741 cgacagctca tctaacatat tagttaccag ttcctcaacg tagtttttta aaaaaatagg
     1801 ttcctcatta tattgagtca tagcatgatc acccccaggt aatgctcgac aaccttattt
     1861 cgaagagcat cctatatata ttctatttac cttgattatg tttattatat cataagtgca
     1921 agcctcgatt ggcttgcttc acaccgattc aataaacgca ttggataaaa cccataggtt
     1981 ttatctcctt gattgcgttt attatatcac attttgtcaa aagattgtta aatacttatc
     2041 tattgtttat tccggtcttt ttactgcgta gccatcgatg aacacccaaa ccgccttggt
     2101 acgaaaatcc aagggatgtc cgtcgaagat agctatatcg gcatccttac ctggctccag
     2161 gctccctacc ctatcatcta ttcccgtaat ctcggcggcg tttatggtaa tggattttag
     2221 agcctccatt tcatccatgc cctccttgac ggctatggcc gcgcataccg gcaggtattg
     2281 tatgggaata accggatgat ccgtcatcat ggctaccttg acccccgcct tggatagaac
     2341 ccccggggcc ttaaatgtca tattcttgag ctcaacctta ctgcgatcgc tgaacaaggg
     2401 ccctacgatc accttggcgt tttcttccaa tagaaaatcc gaaattctat ggccctcggt
     2461 acaatgttcc agtgtcatat ccaaatcgaa ttcgttcctg attctgagtg cagtcaaaat
     2521 atcatctgcc ctgtgggcat gggcttttag gggtatttct ttgtttaata cctttaccag
     2581 aatctccatc ttcaggtccc tgtccggcat cttgtcggga tc
//
LOCUS       DASRNX010000189         3901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_723315,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000189 DASRNX010000000
VERSION     DASRNX010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723315"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VFD89_07635"
     CDS             <1..918
                     /locus_tag="VFD89_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZJ58089.1"
                     /translation="ILSGILRGLGDSVSALVFLIISTLLNVVLDIWFVAGLKMGVPGV
                     ALATVIAQGVSAILCIIKLLKMRDTFDLNLEMLRPVKEYSAQVMRLGLPSGLTQAIFA
                     LAMIAVQSLTNSFGEMVIACNVIVMRVDGFAMMPNFTFGNAMTTFAGQNVGAGKIDRV
                     DKGSGDGIKLAVSVSTVITIIILIFGKHLVRIFTDTSELVDLSMHMMRILAVGYIAMA
                     VTQSLSGVMRGAGDTVTPMWISLITTIILRVPIAYTIAYFTRSAAYPTGRPEATYVSL
                     LVSWVFGAIITILFFRKGNWRKKGLANQQ"
     gene            1148..2071
                     /locus_tag="VFD89_07640"
     CDS             1148..2071
                     /locus_tag="VFD89_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019154632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58090.1"
                     /translation="MELLRIQGLTKTIKKKKILDNISLTVNSGEIVGFLGPNGAGKTT
                     TIKLIMGLFTITEGHINVCGHDIVTDFEAAMENVGGIIENPDLYKRLTGRQNLEYFAS
                     MYKNVNKENIDNIVDMVKMSGRINDKVKTYSLGMSQRVGIAQALLHNPRLLVLDEPTN
                     GLDPLGIKELRDLLKYLAKEAGVGVFISSHLLSEMELMCDRIYIIDEGVIIGEKAIHE
                     EEETVKDDIYEYTLLTSDRDKTLEYLNSLQANCRVENGNVIVVMKRSEMPNIIKNLVY
                     RDIGIYAASEKHRTLEQDFITITTGTKTQIR"
     gene            2103..>3901
                     /locus_tag="VFD89_07645"
     CDS             2103..>3901
                     /locus_tag="VFD89_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZJ58091.1"
                     /translation="MLFLRQVRFEIKNILRSKFILIIGILILAICIATPIISLIGLRS
                     SKNEGMYYGYSTGMGGMGMIRSVAYDMPWYPGPGGYEEDPIIVDGVTIKYDNPFFWNV
                     KSLIDERGYMENDKGNFSDPKVLDIILSTMDAEQEYFVYMAEHITDHRDYRVELSWAG
                     RESIYDKFAYEHTNLPKEQLYEAMSYRMGYDPEVFDEKFINITPEQRLAAIDEIDEQL
                     NLLYKVVETGDFPLFIDLRIKQETKNIEDIKERITVQEKIIIDDPSQEEWVSQYIMDM
                     NNEIDLIETNNIPTLEYRLENNIIPGDDIWQNTALSNIEMNKRNLMYNKILTEEEFNK
                     DRYMVEEYKTYRRYESIMQEERDGYNNQIIIAERSLDAGKPDMNFEPWGARSRTFNFL
                     SYSSFVAMFAILIGGWLMASEFQQGTIRLLMIRPKTRTKILMAKFLSSFIIFLGLYAL
                     GSLLNMVTNGACFGFKDFLYPNFTISGETNFFAYYLPKFAVCIMPAIFTFSLSFLLSV
                     LIRNIAVAIIIPIALFIGSTILMQLLVYGNSVAKWLAFTPIPYMDFTNFFSQYSIVKN
                     AIQKGIPLGIGYGILMLLGLSVVFVIFSIFTFK"
BASE COUNT         1149 a          775 c          935 g         1042 t
ORIGIN      
        1 attctgtcgg gaattttaag agggctaggg gattcggttt cagcactggt atttttaatt
       61 atttctactt tactaaacgt ggttttagat atttggtttg tcgcaggatt aaaaatgggg
      121 gtacccgggg tggccctggc aacggtcatc gcccaggggg tatcggcaat tttgtgtata
      181 ataaagctcc ttaagatgag ggatactttt gacctgaatc tagaaatgct gaggcccgta
      241 aaagagtatt cggcccaagt tatgaggctt ggattaccct ccggtctaac ccaggctatt
      301 ttcgccttgg ccatgattgc ggtgcaatct ctgaccaaca gctttggcga aatggtcatt
      361 gcctgtaacg ttatcgtaat gcgggtagac gggtttgcca tgatgcccaa cttcaccttc
      421 ggcaatgcca tgacgacctt cgccggacaa aacgttgggg ccgggaaaat tgacagggta
      481 gacaagggat caggtgacgg gatcaaactg gctgtaagcg tatcaacggt gattactata
      541 attatattga tcttcggtaa acatctggtg aggatattta ccgacacctc ggagctggtg
      601 gatttgagca tgcacatgat gcggatcctg gcggtgggtt atatagccat ggctgttacc
      661 cagagcctat ccggtgtaat gcggggggca ggggataccg taacccccat gtggatctcg
      721 ctgatcacca ccattatctt gagggtaccc atagcctaca ctatagccta ttttaccaga
      781 agtgcggcat atcccacagg aagacccgaa gcaacctacg tgtccttgct tgtttcctgg
      841 gtctttgggg cgattattac tatactcttc tttaggaagg gcaattggag aaaaaagggc
      901 ctggctaatc aacaatgaaa gcgtaacaat gacaaggatt taacgaattg cttacaaatt
      961 ccttacaaat ggaaacatac cttttattgc catagtataa gatgtacaat atgacatatg
     1021 catataggtt ctgctgcaga tctacaatat taaatatttt ttttgggact catcatttgg
     1081 aacttatatt ctatataaac cgtctaagta tataaacgca catgtatatg tgggaaagga
     1141 attgccgatg gaattattaa gaattcaggg tcttacaaag acaataaaaa agaagaagat
     1201 actcgacaac atctctttaa cagtgaattc cggtgaaata gtggggtttt tagggcctaa
     1261 cggggccggt aaaaccacca ccatcaagct tatcatgggt ctctttacca tcaccgaggg
     1321 tcacataaac gtttgtggac atgacatcgt tacggatttt gaggctgcca tggaaaatgt
     1381 gggggggatc atagaaaatc cggacctata taagaggctt acgggcaggc agaacttgga
     1441 gtactttgca tccatgtaca aaaatgtgaa caaggagaac atcgataata tagtggacat
     1501 ggtaaagatg tccggaagaa tcaacgataa ggttaagaca tattccctgg gcatgagcca
     1561 gcgggtgggg atagcccaag cgctgctcca taatcctcgt ctattggtat tggatgagcc
     1621 caccaacggg ctggacccct tgggcattaa ggaactgagg gacctcttaa aataccttgc
     1681 gaaagaggca ggggtaggag tatttatatc aagccatctg ctttccgaga tggagcttat
     1741 gtgtgacagg atttacataa tagatgaagg ggtaattatc ggagaaaagg ccatccatga
     1801 ggaagaggaa acggtcaagg acgatatcta tgaatacacc cttctaacca gcgacaggga
     1861 caagaccttg gagtatctaa atagcctgca ggcaaattgc agggtagaaa acggtaacgt
     1921 tatcgtcgtt atgaagcgaa gcgagatgcc aaacatcata aagaatcttg tatatcggga
     1981 tataggcata tacgctgcca gtgaaaagca taggaccctt gagcaggatt tcatcaccat
     2041 taccaccgga acgaaaacac agatcagata atttccaatt aaattttaag ggggatatgt
     2101 cgatgctttt tttaagacaa gtcagatttg aaattaaaaa tatactaaga tccaagttta
     2161 tattgattat cggtatcctg atactggcca tatgtatagc cacacccata attagcctaa
     2221 tcggtctaag atccagcaag aacgagggga tgtactatgg ctatagcacg ggtatggggg
     2281 gcatgggaat gattaggtct gtcgcctatg atatgccctg gtatccgggt cccggcggat
     2341 acgaggagga ccctataatt gtagatggtg taaccatcaa atacgataac cccttttttt
     2401 ggaacgtgaa gagcctcatt gatgagcggg ggtatatgga aaacgataag ggcaattttt
     2461 ccgaccccaa agtgttagac attatactct ccaccatgga tgcggaacag gaatactttg
     2521 tatacatggc agagcatata acggaccata gggattaccg cgtcgagcta tcctgggccg
     2581 gcagggagag tatctatgat aaattcgcat atgaacatac taatctccct aaggagcagc
     2641 tctatgaggc catgtcctac aggatggggt atgatcccga ggtgtttgat gagaagttta
     2701 tcaatattac ccccgaacaa aggctggcgg ccatagatga gatagacgaa cagctgaact
     2761 tgctttataa agtggtggaa accggcgatt ttcccttatt tattgacctg cgtatcaagc
     2821 aggagacgaa aaacatagag gatattaagg aaagaataac cgtgcaggaa aagatcataa
     2881 tcgacgatcc gtcccaggag gaatgggtca gccagtatat aatggatatg aacaatgaaa
     2941 ttgatctcat tgagaccaac aacattccca ccttagaata cagacttgaa aataatatca
     3001 taccggggga cgacatctgg caaaataccg cactttccaa tatagagatg aataagcgaa
     3061 atcttatgta caacaaaatc cttacggaag aggaatttaa caaggacagg tacatggtcg
     3121 aggagtataa gacatacagg cgatatgaga gcatcatgca ggaagaacga gacgggtata
     3181 acaaccagat aataattgca gagcgttcat tggatgcagg taagccggat atgaactttg
     3241 aaccttgggg cgcaaggagc agaaccttta actttttgag ctattcttcc tttgtagcca
     3301 tgtttgccat ccttatagga ggctggctaa tggccagtga atttcaacag ggtaccatca
     3361 ggctgctgat gatcaggccg aaaacccgta caaagatatt gatggccaag ttcctatcct
     3421 cctttattat cttcttgggc ctatatgcct tgggaagcct tctcaatatg gtcaccaacg
     3481 gtgcctgctt tgggtttaag gattttctat acccgaactt caccatatcg ggggagacga
     3541 atttctttgc ctattatctg ccgaagtttg cggtttgcat tatgccggca atctttacat
     3601 tctccctatc cttcttgtta tcggtactca tcagaaatat agcagttgcc atcatcatcc
     3661 ccatagcgtt atttatcggc tctaccatat tgatgcagct gttagtttac ggtaacagcg
     3721 tagctaaatg gcttgccttt acgccgattc cctatatgga ctttactaat ttcttctccc
     3781 agtattctat agtaaagaat gccatccaaa agggtatccc actgggcata gggtatggta
     3841 tactgatgtt gctgggtttg tctgttgtat ttgttatatt ttctatattt accttcaaga
     3901 g
//
LOCUS       DASRNX010000190         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_195273,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000190 DASRNX010000000
VERSION     DASRNX010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195273"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..962
                     /locus_tag="VFD89_07650"
     CDS             219..962
                     /locus_tag="VFD89_07650"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZJ58092.1"
                     /translation="MRDTFLFDLDGTLLPFDIDEFIYIYFKEMGKHFHDMIDGKLLAE
                     YIMASTEAMIGNLEPLVNEEKFMLHFSGLIGEDALQKYKDRFDLFYDDRFDAVKECVA
                     HVPEIVKSIEVLKDKGYELVVATNPIFPLKAVHKRIEWAGLCVDDFSYISCYEKNSYC
                     KPNIQFYEEVLDAIGKEPENCYMVGNDVQEDLVAGELGIETYLITDYLIDRTRGEVES
                     TYKGNYRDFYEFVKGLEPVRDFEKVRYSI"
     gene            1073..2551
                     /gene="rhaB"
                     /locus_tag="VFD89_07655"
     CDS             1073..2551
                     /gene="rhaB"
                     /locus_tag="VFD89_07655"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HZJ58093.1"
                     /translation="MRTLDLLAFDFGASGGRGILGRFDGEKLAMDELHRFSNDPVEVG
                     GHLYWDVLRLFWNMQEALLKYGARENGSFASLGIDTWGVDYGLLDRDGELLGNPYHYR
                     DVRTEDMMEEAFTKMPREMIFQKTGIAFQKFNTLYQLLSTVIKKPDMMDRAETLLFMP
                     DLLAYFLSGEKSTEFTEASTGQLLDANTGHWCGDLLEAMSIPKNIFTNIQQPGSLRGR
                     IKADLAKTLGVGSVPIIAVASHDTGSAVAAVPNTEGNYAYLSSGTWSLMGVEVDKPVI
                     NEKTLGWNYTNEGGVNGTYRLLKNIMGLWIIQECKREWDRRGEVYSYDEMAAMASAAA
                     PFKAFIDPDHDSFYSPGEMPKKIQKFCRDTGQWVPETKGEILRCVFESLAMKYRWSLE
                     KLEEITEKSLDVLHIVGGGSQNGLLNQLTANAISRPVICGPVEATAIGNLMVQAMALG
                     EVANLREIRQVVKRSFPTEDYLPKDTRAWDSAYEEFSKHNEG"
     gene            2583..>2890
                     /locus_tag="VFD89_07660"
     CDS             2583..>2890
                     /locus_tag="VFD89_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HZJ58094.1"
                     /translation="MGNTKPGGECIWYIPDCYYPEITTEGHYVSHEAICVLNTGLEDA
                     NLSITLYFEDREPLTDFKAECRAERTNHIRLDMIVDEKGNGIPKGVPYAVMVESNVPI
                     "
BASE COUNT          829 a          552 c          797 g          712 t
ORIGIN      
        1 gtcagggact gagccaaagg taaagctata cttctctgcc ataggcagcg acgcaaaaga
       61 ggctaggagc aagatggaaa atctcaggga tgccgtttgt gccagggttt gaaattgcac
      121 acaaccgttg caattatcat aacgataaat ttagttaagg acaaggtagt ttattttttt
      181 aacacactac gactagaatt gaaccggagg ttacagctat gcgtgacaca tttttatttg
      241 acttagacgg tacattgctt ccctttgata tagacgagtt tatctatatc tactttaagg
      301 agatgggcaa gcactttcat gatatgatag acggaaaatt gctggcggaa tatatcatgg
      361 catccacgga ggccatgata ggaaaccttg aaccgttggt aaacgaagaa aagtttatgt
      421 tgcatttttc agggttaata ggggaggatg ccctacagaa atataaggac agattcgacc
      481 tgttctatga tgacaggttt gatgcggtca aggaatgtgt agcccatgtt ccggaaattg
      541 ttaagagcat agaggttctg aaggacaaag ggtacgaatt ggtggtagct accaacccta
      601 tctttccttt aaaggctgta cataagcgga tagaatgggc aggcctttgc gttgatgact
      661 tttcctatat ctcctgttac gagaaaaatt cctattgtaa gcccaatata caattctatg
      721 aggaggttct ggatgctatc ggtaaggaac cggaaaattg ttatatggtg ggtaacgacg
      781 tccaggagga cctagtagcc ggcgagctgg gtatagagac ctatctgata acggactatc
      841 tgatagacag gactaggggt gaggttgaga gcacctacaa gggaaattac cgggactttt
      901 atgaattcgt caagggtctt gagcccgtta gggattttga aaaggtgcgc tattccattt
      961 aaagggtcca tcacttccaa gttgaaacct atgtgatgta atgaccagag caattgctta
     1021 aggtgctaaa atatagccgg cccggtgatg ccggtatggg ggaggggagc atatgagaac
     1081 attggatttg ttggcctttg attttggtgc cagcggcgga aggggaatat taggacggtt
     1141 cgacggggaa aaactggcta tggacgaact ccataggttt tctaacgacc ccgttgaggt
     1201 agggggacat ctctactggg atgtattaag gcttttttgg aatatgcaag aggcactttt
     1261 aaagtatggg gcccgggaaa atggcagctt tgcatccctg ggcatcgaca cctggggtgt
     1321 ggattacgga ttgttagaca gagacgggga actattgggt aacccctacc actacaggga
     1381 tgtacgcacc gaggatatga tggaagaggc ctttaccaag atgcctaggg agatgatctt
     1441 tcaaaaaacg ggtatcgcct ttcaaaagtt taataccctt tatcaattgc tgtctacggt
     1501 tattaaaaag ccggacatga tggatagagc cgagaccctg ctcttcatgc cggatctttt
     1561 ggcctatttt ttgtctggtg aaaaatctac cgagttcaca gaagccagca cggggcagct
     1621 attggatgca aataccggac attggtgtgg tgacctttta gaggctatgt caatccctaa
     1681 gaatattttc acaaatattc agcagcccgg cagtctgagg ggcaggataa aagcggactt
     1741 agctaagacc ttgggggtgg gcagcgtgcc catcatcgca gtggcatccc atgataccgg
     1801 atctgcagtg gcagctgttc ctaacacgga aggtaactat gcctacctga gcagcggaac
     1861 ctggtccctt atgggggtag aagttgataa gcccgttata aacgagaaga ccctgggatg
     1921 gaactatacc aacgaaggcg gagtaaacgg aacctacagg cttttaaaaa atatcatggg
     1981 cctgtggatt attcaggaat gcaagaggga atgggatcgc aggggtgagg tttacagcta
     2041 tgacgagatg gcggctatgg catcggctgc tgcgcccttt aaggcattta tagacccgga
     2101 ccacgacagc ttttacagcc cgggggaaat gcctaaaaaa attcagaagt tttgcaggga
     2161 cacagggcag tgggttccgg aaaccaaagg cgagatttta aggtgcgtat tcgaaagttt
     2221 ggccatgaag tatcgctggt cgttggagaa gcttgaggag atcacagaga aaagtctgga
     2281 tgtcctccac atcgttggtg ggggcagcca gaacggactt ctaaatcaat taaccgctaa
     2341 cgccatatca cggccggtca tatgcgggcc cgttgaggca acggctatcg gcaacctcat
     2401 ggttcaggcc atggcccttg gtgaggtagc aaaccttagg gaaatccgtc aagtagtcaa
     2461 aagatccttt cctacggaag attatttgcc aaaagatacg agggcatggg acagcgcata
     2521 tgaggagttt tcaaagcata acgaggggta attttattga aataaaagag agggtggggt
     2581 aaatgggaaa cacaaagccg ggaggagaat gcatttggta catcccagat tgctattatc
     2641 cggaaataac aactgagggg cactatgtaa gccatgaggc catatgcgtt ttaaatactg
     2701 gcctagagga tgccaacctc agtataaccc tatactttga ggatagggaa ccattgacgg
     2761 actttaaggc ggagtgcagg gccgagagaa caaaccatat tcgtctggat atgatagtgg
     2821 acgaaaaggg taacggaatc cctaagggtg tcccctatgc cgttatggta gagagcaatg
     2881 tacctataat
//
LOCUS       DASRNX010000191        12904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_462334,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000191 DASRNX010000000
VERSION     DASRNX010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462334"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(436..1605)
                     /locus_tag="VFD89_07665"
     CDS             complement(436..1605)
                     /locus_tag="VFD89_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZJ58095.1"
                     /translation="MYRIRILNNISQNGLELLPSKFYDVSPDEPAPHGILLRSHNMHD
                     MEVPPSLLAVARAGAGVNNIPVDQYTEKGIVVFNTPGANANAVKELVLAGIFLSSRKI
                     VEGVEWANSLKGKGKDVASLVEKGKSNFVGPEVKGKKLGVIGLGAIGVLVANDAHAVG
                     MEVMGYDPFISVQSAWGLSRGIHRADNLDDLLSESDYISIHIPLMDKTKGIINKEAIS
                     KMKKGARLLNFSRGQLVNDDDLLEAIENGHISKYVTDFPNDKLLGVENIIPIPHLGAS
                     TPESEENCAHMAVSELRNYLENGNIANSVNYPECELPPCPDTVQRITVAHKNVPNMVG
                     QITSRLAAEHINISNMINKSKGNYAYTIMDLNTRVGDDILDELSNIPGNIRVRSI"
     gene            complement(1643..2725)
                     /gene="serC"
                     /locus_tag="VFD89_07670"
     CDS             complement(1643..2725)
                     /gene="serC"
                     /locus_tag="VFD89_07670"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HZJ58096.1"
                     /translation="MKPVYNFSPGPSALPKPVLEKAQKELLDYKGTGMSVMEMSHRSK
                     LFQEIVDDAQELLKGLMGIPDNYKILFLQGGATTQFSMVPMNLFTENKKADYAITGSF
                     AKKAAQEAKRYGTVNVVASSADGNFSYIPDVESMKVSPDSDYFHITTNNTIYGTKFTE
                     LPDTGSVPLVADMSSNILSEVFDVSKFGLIYAGAQKNIAPSGLTVVVLREDLMGNAMD
                     ITPTMLDYKIQADANSLYNTPTTFSIYMAKLVFEWLKDLGGIPAIGEINREKANLLYG
                     CIDDSTIYKGTANPKDRSLMNVTFTLPSEDMTAKFVKEAENNGLVTLKGHRSVGGIRA
                     SIYNAMPIEGVRALVDFMQEFELSNK"
     gene            complement(3327..4379)
                     /locus_tag="VFD89_07675"
     CDS             complement(3327..4379)
                     /locus_tag="VFD89_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZJ58097.1"
                     /translation="MKHIFNQKFIWLTVAILVFVVFLGGCGQQNRVTLKVYNWGEYID
                     KDVIKDFEKEHGIRVIYTTYATNEDMYVSMKHSGSTYDVAFPSDYMISRMIDEDMLHK
                     IDMNNIPNYKYIDDIFKDLSFDPNNEYSVPYMWGTLGIVYNKTMVDDPVEGWDILWNE
                     KYSKQILMLDSQRDSIGLTLQMLGYSMNSRNVEELEEAKEKLMEQKPLVLAYVGDEVK
                     DKMVGGEAALAVVWSGDAVAMKWENPDLEYVLPKEGGNLWFDSAVIPKTSQHQKEAEL
                     FINFLCETDIAFRNTDYIGYSTPHVEAMGMLDDELLSDSAAYPSDEDIMHSEVFEDLS
                     DTLDVYNRIWTEIKAK"
     gene            complement(4376..5161)
                     /locus_tag="VFD89_07680"
     CDS             complement(4376..5161)
                     /locus_tag="VFD89_07680"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZJ58098.1"
                     /translation="MMRHIKRFYIGIIFFFLYAPILILMIFSFNNSRYMSRWDGFSLK
                     WYADLFSDRDILSALYYTVLIAILSTIIATVIGLVAAVGIHDMNKLGKGIVINISYLP
                     VLNPDIVTGISLMMLFVLMNMEPGFLTLLLAHITFNIPYVIFSILPKLKQMNKHLYEA
                     ALDLGATPFYAFRKIILPEIMPGVVTGMILAFTLSIDDFVISYFTTGAGVSNLSIIIY
                     SMARVGIKPTINALSTLLFVSVLLLLLIVNKRTTNNTGKENVL"
     gene            complement(5158..5982)
                     /locus_tag="VFD89_07685"
     CDS             complement(5158..5982)
                     /locus_tag="VFD89_07685"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZJ58099.1"
                     /translation="MKKTWIAYPYVVWMILFIVVPLMLIVFYGAAKTTPEGIEFSLEH
                     FQRFFEPLYIMVLIRSLYLALISTIVCLLLGYPVSMILASREYRDKRILIFLMVIPMW
                     MNFLLRTYAWLTLLEKNGIINTFISFLGLPKMDLLYNQWAVVLGMVYNFLPFMVLPIY
                     SVLIKIDRSVIEAAEDLGANSGTVFRKIIFPLSLPGVVSGITMVFMPAVTTFVISRLL
                     GGGQFLLIGNLIEELFLKSGEWGFGSALSIIMMILILFSMAIMSKYEKENEGGGLL"
     gene            complement(5979..7025)
                     /gene="potA"
                     /locus_tag="VFD89_07690"
     CDS             complement(5979..7025)
                     /gene="potA"
                     /locus_tag="VFD89_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1902047 - polyamine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HZJ58100.1"
                     /translation="MSYEIINLIDISKAFDDTLVLENIDLYIRRNEFLTLLGPSGCGK
                     TTTLRIIGGFEDPTTGKVLFEGKDITHIPPYKRKLNTVFQRYALFPHMNVAENIAFGL
                     NIKKLAKSIINEKVRDMLSLVNLRGFEKRSVDSLSGGQQQRVAIARALINEPEVLLLD
                     EPLGALDLKLRKGMQIELKNIQQKLGITFVYVTHDQEEALTMSDTIVVMNEGRIQQIG
                     TPIDIYNEPNNAFVADFIGESNIVDGIMLEDFVVNFAGYVFKCLDGGFDRDEKVDVVV
                     RPEDIKLVSPEQGMLKGRVISVIFKGVHYEMMIDSNGIMWKVHNTLMEPVDTMVGINI
                     FPDDIHIMKKVMAP"
     gene            complement(7137..7676)
                     /locus_tag="VFD89_07695"
     CDS             complement(7137..7676)
                     /locus_tag="VFD89_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZJ58101.1"
                     /translation="MNIGEKIKHLRIKNGLTQQELANRCELSKGFISQVERDLTSPSI
                     ATLVDILECLGTNLKDFFNESSNEKIVFHKDDVFLQEDQDLGHSIHWIVPNAQKNRME
                     PILMRIQPGGSSEPDSPHDGEEFGYVVAGSVFLHLGNQKLKVKKGESFYYKPSLVHYL
                     SNESRFPATIIWVSSPPSF"
     gene            8428..9522
                     /locus_tag="VFD89_07700"
     CDS             8428..9522
                     /locus_tag="VFD89_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ58102.1"
                     /translation="MSAQTQDAKLRIGIIGCGGIANGKHMPSLAKVEEVEMVAFCDII
                     VERAEKAAKEYGTPDAKVYADYKELLKDESLDVVHVCTPNKSHADITIDALESDKHVL
                     CEKPMAKTAADARRMVEAAKRTGKKLTIGYQNRFNPESQYLHGTCRRGDLGDIYFAKA
                     HAIRRRAVPTWGVFLNEEEQGGGPLIDIGTHALDLTLWMVDNYKPKTVVGTTYRKLAE
                     QRDTANAWGDWDPEEFTVEDSAFGFVVMENGTTIILESSWALNTLDVGEAKTTLCGTK
                     AGADMRDGLRINGVEFNKMYTKKPSLGAGGVDFYDGEADDPSYLEARAWIDAIINDTD
                     PVVLPEQALAVTEILEAIYESAKTGKAITF"
     gene            9718..10683
                     /locus_tag="VFD89_07705"
     CDS             9718..10683
                     /locus_tag="VFD89_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZJ58103.1"
                     /translation="MKLGVMTVLLGNMKAEEAFKYLSESGVQAVELGTGGYPGNAHAN
                     PDELLKDDGKIEELKNLVDKYNLEISSLSCHGNPVHPQKEIAAEFHGDFEKTVLLAEK
                     LGVTRVNTFSGCPGDSPNSMYPNWVTCPWPEDFLKVLDYQWNDVLIPYWEKTAAFAKD
                     HGVDMICLEMHPGFAVYNPETLLKLRAAVGDAIGANFDPSHLFWQGIDPVAAIRELEG
                     AIYHFHAKDTKIDARNTAVNGVLDTKHYGDEINRSWIFRSVGYGNDYQMWKDMVSTLR
                     MVGYDYVLSIEHEDSLMTPNEGLQKAIAFLKEVMVFEDKGEMWWA"
     gene            11043..11744
                     /locus_tag="VFD89_07710"
     CDS             11043..11744
                     /locus_tag="VFD89_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006838620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HZJ58104.1"
                     /translation="MTKTNFTEADLYKPIYEYLTRLGYTVRGEVKDCDVVAIKGEELI
                     IIELKKGFSVELLTQAAARQRITSGVYVALPRPKASLRSSKWKGIRLLLRRMELGLIF
                     VNMNLDKPKAEVILHPLPYKLRRAKKAREGIISEAEGRSGNFNPGGSNRKKIMTAYRE
                     NAIFIACCLERDDILSAAELRKLGTGSKTYSILYNNFYGWFHRVARGKYSLSEAGKSA
                     LAQYPEIADHYREKL"
     gene            complement(11739..12764)
                     /locus_tag="VFD89_07715"
     CDS             complement(11739..12764)
                     /locus_tag="VFD89_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HZJ58105.1"
                     /translation="MNRVKGILKKGLKRYGLDAFSAMALGLFSSLIIGLILSQLSRIP
                     GLGFISDFSDILSAQSPVVGAAIGVAIAHGLKAQPLVLFSSAATGAFGYSYMNAGPMG
                     AFMAAVIGAELGRLIAGKTRVDIVLVPSVTILAGGLAGKFIGPYISGFMVWLGHVINT
                     ATTLQPFPMGILVSTSMGMILTAPISSAALAISTQMTGLAAGAATVGCSTQMIGFAVA
                     SYRENKVGGLIAQGLGTSMLQVPNIIRRPQIWIPPTLASAILGPLSTTILKMQGNPIG
                     AGMGTSGLVGQFAVWATMDGTIPTWLLLTEILMMHFILPALLSLVISEIMRKRGWIKY
                     GDMELQL"
BASE COUNT         3652 a         2822 c         2939 g         3491 t
ORIGIN      
        1 ataagaaccg ataatagggc atcataaaac atcagaaagc attggggaac attggggata
       61 tcacaaaagg cccatggaat ctcataaagc acttgaaaca cctgaaacat ttgaaacccc
      121 atacgtggca tggcgaaata attaaaaaaa agcgaagaaa agacaaaggg cgaataagaa
      181 aacaaaatac gaatggaaag cagataaaag atcaataaaa aatcaataaa aggtcaacaa
      241 aaagccgctg actatattac aagttcagcg gctttgcttt taaatacatc atccctttga
      301 ctaagcaatc catttacctt cccattaacc cattgcttaa tttcccatta atccatcgcc
      361 ttaattaccg gtaaaggaac tacaaaaggg acaattttcg aaggtaaatc atctgtaaat
      421 acagatttct tatacctata tcgagcgcac cctgatattt ccggggatgt tggatagctc
      481 atctaggata tcgtccccaa ccctggtgtt tagatccata atggtatagg catagtttcc
      541 cttgctcttg ttgatcatgt tggagatatt tatgtgctct gcagccagac gggaggttat
      601 ttggcccacc atattaggaa cattcttatg ggctacggtt atcctctgta ctgtatcggg
      661 gcaagggggt agctcacatt cggggtaatt tacggaattt gcgatattcc cgttttcaag
      721 gtaattgcgg agctcactga ccgccatatg ggcacagttc tcttcagatt ccggggtaga
      781 agcgccaagg tgggggatgg gtataatgtt ttccacccca aggagcttat cgtttggaaa
      841 gtcggtgaca tatttgctga tatggccgtt ttcgatggcc tctaagagat catcgtcgtt
      901 gaccagctgt ccccgggaga agtttagaag ccgtgcgcct tttttcatct tggatatggc
      961 ctctttgtta ataatcccct tggtcttgtc cataagtggt atatgaatgg agatatagtc
     1021 ggactcggat agaagatcat ccagattgtc ggccctgtgt ataccccttg ataatcccca
     1081 ggcagattgc acggagatga aggggtcgta tcccataacc tccatcccta cggcatgggc
     1141 atcgttggca accagaactc cgatggcgcc cagtcctata acacccagct tcttgccctt
     1201 gacctcgggt cctacaaagt tagacttgcc cttttcaacc agggaagcca catccttgcc
     1261 cttaccctta agggagtttg cccactctac accttcgaca atttttctgg aagagaggaa
     1321 tatccccgct aaaaccaatt cctttacggc attggcgtta gccccggggg tgttaaatac
     1381 cactatccct ttttcggtgt actggtcaac aggtatattg tttacacccg caccagccct
     1441 ggcaaccgcc aatagagacg ggggtacttc catatcgtgc atgttatggc ttctgagcag
     1501 gatcccatgg ggtgcaggct catcgggtga tacatcatag aacttactgg gcagcagttc
     1561 caagccgttt tgtgatatat tgtttaatat tctaatccta tacatttgca tcctcttttc
     1621 tatatgctta cggtagacga aatcatttat tgctcagttc aaactcctgc ataaaatcaa
     1681 ctaacgccct tacgccctct atgggcattg cgttatagat gctggccctg atgcctccca
     1741 cggatctatg accctttagg gtaaccaaac cgttgttctc ggcttcctta acgaatttgg
     1801 cggtcatatc ctcactgggt agggtaaatg taacgttcat caaggaccgg tccttgggat
     1861 tggcggtacc cttgtatata gtggaatcat ctatgcagcc atagagaagg ttagcctttt
     1921 ccctgttgat ctctccgatt gcaggaatcc cgccaaggtc ctttagccac tcaaagacca
     1981 gcttggccat atatatgcta aaggtggtgg gggtattgta cagagaattt gcatcggcct
     2041 gtatcttata atccagcatg gtgggcgtaa tgtccatggc attgcccatg agatcctcgc
     2101 ggagtacgac tacggtgagg ccggaaggcg ctatgttctt ctgggcgcct gcgtagatga
     2161 gaccgaattt ggatacgtca aaaacctcag acaaaatatt agacgacata tccgctacca
     2221 gaggcaccga accggtatcg ggtagttcgg taaacttggt cccatatatg gtattattgg
     2281 tggtgatatg aaaatagtcc gaatccgggc tgaccttcat ggattctaca tccggaatat
     2341 agctgaagtt gccatcggca gaggaggcca ccacattgac tgtcccatag cgtttagcct
     2401 cttgtgcagc ttttttggcg aaagagccgg taatggcata gtccgctttt ttattttctg
     2461 taaataagtt catgggcacc atggaaaact gggttgtggc gccgccttgt agaaatagaa
     2521 ttttgtagtt atccggtatg cccataagtc ctttaagcaa ttcctgggca tcatcgacta
     2581 tctcttgaaa caattttgaa cgatggctca tctccatgac ggacatacct gtgcccttat
     2641 aatcaagcag ttctttttgg gctttttcca gcacaggctt cggcagtgca gacggtcccg
     2701 gcgaaaaatt ataaactggt ttcatttgga tcctcctcgt taatagtaat aatattttta
     2761 ggttgcgcaa taaattaagg acaacctatc aagcaaagtt gcatatatcg ttgtatgatg
     2821 caataagtat atgtttgacg acaaatacaa tacgacatat ttcagatatc aaaaacatat
     2881 ttcttatcta tatgctttac gacaaacatt ctacattagt atacgggtgt cgtaaaacat
     2941 tatcgtcaat tcatatgtta cggaaaaata cattgcttta acgcaaaatc aaataactta
     3001 actgattttg tattgaaaga tctttttcgt aacatatctc gtatgatata gaaaagccat
     3061 tgttttaggt ataatcaaag ccgcatatta agaatataac cattatacta tatattttac
     3121 taaagtaaac tactgaatgg atgaaattta tcattttttt acatagattg tgaaaaaaat
     3181 agctatgggt agaaaatcag ccccgactag agttttgtcg ccctaatcct tgtatacaga
     3241 tagggcaacc caaaatatag ccatatttcg ggctgcccat taaaatccca ttgttccata
     3301 tataattgct cgaagcagag gctattttat tttgccttaa tctcggtcca aatcctgttg
     3361 tatacatcca aggtgtcgct taaatcttca aagacctcag agtgcataat atcctcatcg
     3421 ctggggtatg cggcgctgtc gcttagaagc tcatcgtcca gcatgcccat agcctccaca
     3481 tgaggggtgg aatagcctat atagtcggta tttctaaagg caatatccgt ctcacataga
     3541 aaattaataa ataattccgc ttctttttga tgttggctgg tcttcggaat aacggccgaa
     3601 tcaaaccata ggttgccgcc ttctttggga aggacgtact ctaaatcggg attctcccat
     3661 ttcatagcca cggcatcccc ggaccatacc acggccaaag cggcttcgcc gcccaccatc
     3721 ttatccttta cttcatcgcc gacataggca aggaccaagg gcttttgttc cataagcttt
     3781 tctttggctt cttcaagctc ctctacattc ctgctgttca tagaataccc cagcatctga
     3841 agtgtaaggc cgatggaatc tctctggctg tccagcatca ggatctgctt tgaatacttt
     3901 tcgttccaaa ggatatccca gccttctacc ggatcatcta ccatggtctt attgtatact
     3961 atccccaagg ttccccacat atagggcacg gagtactcgt tgttcggatc aaaggacaga
     4021 tccttgaaga tatcgtctat atatttgtag ttgggtatat tgttcatatc aattttatgg
     4081 agcatatctt cgtcgatcat cctgctgatc atatagtcgg aggggaatgc aacatcgtag
     4141 gtgctgccgc tgtgcttcat gctcacatac atgtcctcgt tagtagcata ggtggtgtat
     4201 attaccctta taccatgctc cttttcaaaa tccttgataa catccttgtc gatgtattcc
     4261 ccccagttgt acacctttaa ggtcacgcgg ttttgttgtc cgcacccgcc aaggaacacg
     4321 acaaagacta gaatcgctac tgtcagccaa ataaactttt gattaaatat atgtttcata
     4381 gaacattctc ctttccggta ttattggttg ttctcttatt tacaatgagg agcaataaaa
     4441 gaacagagac aaacaagaga gtcgataggg cgttaatggt gggcttgatt cctaccctgg
     4501 ccatagaata gatgatgatg gaaaggtttg acacaccggc accggtggta aagtagctta
     4561 taacaaaatc atctatggac agtgtaaagg ctaggatcat cccggtaacc acccccggca
     4621 ttatttccgg tagtataatc tttctgaagg cataaaatgg ggtagcaccc aaatccaacg
     4681 ccgcctcgta cagatgcttg ttcatctgtt ttagtttagg taatatagaa aaaataacgt
     4741 agggtatatt aaaggtaata tgggccagca aaagggtcaa aaaccccggt tccatattca
     4801 taagaacaaa gagcatcatt agggatatcc ccgtcactat atcgggattg agcaccggaa
     4861 ggtacgagat gttgatcacg ataccctttc ccagcttgtt catatcgtgt atcccgactg
     4921 cagccacgag gcctatgacg gtagcgatga ttgtagacaa tatggctatt aggactgtat
     4981 agtagagggc cgataggata tccctatcgc tgaacaaatc cgcgtaccac tttagactaa
     5041 acccatccca acggctcata tacctggaat tattgaagga aaagatcatg aggatcagaa
     5101 tcggagcata gagaaagaag aatattatgc cgatatagaa cctctttata tggcgcatca
     5161 tagaagtcca cccccttcgt tttccttttc gtacttggac attatagcca tgctaaatag
     5221 gattaaaatc atcattataa tggagagagc cgaaccaaat ccccattccc cggactttag
     5281 gaagagctct tcaataaggt taccaataag gagaaactgt cctccgccaa ggagcctgga
     5341 gattacaaag gtagttacag caggcataaa gaccatggtt atgccggaga cgacccccgg
     5401 aaggcttaag gggaagatga tcttccgaaa tactgttccg gagtttgccc ccaaatcctc
     5461 cgcagcctct atgacactcc tatctatttt gattagcacc gaatagatgg gaaggaccat
     5521 aaaggggaga aagttgtaca ccatacccag gacaacggcc cattgattat atagtaggtc
     5581 catctttggc agtcctaaaa atgagataaa ggtatttata attccgttct tctcaagcag
     5641 ggtaagccag gcgtaggtcc taagtagaaa gttcatccac atggggatga ccatcaagaa
     5701 tataaggatt cttttatccc tatattccct actggctagg atcatggaca ctgggtagcc
     5761 cagcaggaga cagactatag tgcttataag ggccaaatac aatgacctta taaggaccat
     5821 gatataaagg ggttcaaaaa acctttgaaa atgctccaga gaaaactcta ttccctctgg
     5881 ggtggtcttg gctgccccgt aaaagactat gagcatgagg ggaaccacga tgaataagat
     5941 catccacacc acataagggt acgctatcca tgtctttttc acggagccat caccttcttc
     6001 attatatgga tatcatcggg aaagatattt attcccacca tggtgtcaac gggttccatc
     6061 aaggtgttgt ggaccttcca cataatgcca ttggagtcta tcatcatctc gtaatggacg
     6121 cctttgaata ttacagagat taccctgccc tttagcatac cctgctccgg ggaaaccaac
     6181 ttgatatctt cgggcctgac taccacatcc accttttcat ctctatcgaa tccgccgtct
     6241 aggcatttaa agacatagcc ggcaaaattc actacgaaat cttcaagcat gattccgtct
     6301 actatattgc tctcgccaat aaaatccgct acaaaggcgt tgttaggctc gttatatata
     6361 tctatgggtg tcccaatttg ctggatacgt ccctcgttca taaccacaat ggtgtcggac
     6421 atggtcaggg cctcttcctg atcatgggta acgtagacga aggtgatacc cagcttttgc
     6481 tggatgtttt taagttcaat ctgcatgccc ttgcgaagct ttaaatccaa agcccctaag
     6541 ggttcatcca gaagcaggac ctcgggctca ttgataagcg ccctggctat agccactcgc
     6601 tgttgctgac ccccgctcag agaatcgacg gaccgctttt caaatcccct gagatttacc
     6661 aggctgagca tatccctaac cttctcgttg atgatggact tggccagctt ctttatattg
     6721 aggccgaagg caatgttctc cgctacgttc atatggggga agagggcata cctttggaaa
     6781 accgtattga gtttccgctt gtacgggggg atgtgagtga tatccttccc ttcaaagagg
     6841 accttccctg ttgtaggatc ttcgaaccct ccaattatac ggagggtagt tgtcttaccg
     6901 catccgctgg gtcctaaaag agttaaaaat tcattgcgcc ttatataaag gtctatattc
     6961 tccaaaacaa gagtatcatc gaaagccttg gagatatcta ttagatttat aatttcatag
     7021 gacatttatc ataccccctg aattgtaatg gttagtttat atgcttgtga taaaggctgc
     7081 gtcagaaaac accatacatt acccttaaac ctataaggga tttcttaacc aagagattaa
     7141 aaacttggcg gtgacgagac ccaaataatc gtggcgggaa acctggattc gtttgaaagg
     7201 taatgaacca gggatggctt gtaataaaag ctctcaccct tttttacctt gagcttttgg
     7261 ttgcctagat gcaagaaaac ggaacctgcc accacatacc cgaattcttc cccgtcatgg
     7321 ggggaatcgg gttccgagga gccgcctggc tgtattcgca tcaggatcgg ttccatgcgg
     7381 tttttttggg cattagggac tatccaatgt atagaatgcc ctaaatcctg atcctcttga
     7441 aggaaaacat cgtctttatg gaatactatc ttttcgttgc tgctttcgtt aaaaaaatcc
     7501 ttgaggttgg ttcccagaca ctcaagaatg tccaccaagg tggctatgga cggggaggtc
     7561 aaatcccgct ccacctggga gataaatccc ttggacagtt cgcagcggtt agccagctcc
     7621 tgctgggtga gcccattttt aatcctcaaa tgctttattt tttctccaat gttcatgggc
     7681 attgctcctt tggggttggt tttggataac taaacatttt gtttacatct gctaaacaaa
     7741 actgccaata tatttttaac acaataaaat aaaaatgtca atagtcgatt tttactcaga
     7801 aagcatctag ttctccttag gctgctatat ctgttattaa acgcttcaat taatattaga
     7861 tatggcagcc tattatagaa gaacttgctt tgtggtaaaa atcgatagtc gatttttact
     7921 cagaaagcat ctagttctcc ttaggctacc gcatgtcaca ctgcacggac tatctataat
     7981 aatagaatcc atattttaca gaaaaatggt ttattccaga catctttcaa atcacaccgg
     8041 gaagccgcct tatatgtccc atctttgcgg gattattgac ggcgcttccc tcagaagctc
     8101 atctttgagg tcgttgaaat ctgcttccac aaatccattt tccttagtca atttgagtta
     8161 atgcaatgaa atcatgcttc aattaatatt agatgtggca gcctattata gaagaacttg
     8221 ctttgtggta aaaatcgata gtcgattttt actcagaaag ggcagatatt ttttggcagg
     8281 gttgaaaata ctaagcaaat aatgtaaaat ataatgggcg gagggggaac agatccgaat
     8341 tttccccgga agaagcaggt aggtccataa gttttgtaaa cgcataaggc gaatacaata
     8401 taaatataac ccataggagg aataataatg agtgcacaga cacaggatgc caaattacga
     8461 atcggaataa tcggttgcgg cggtatcgcc aacggcaaac atatgcccag cctagccaag
     8521 gtcgaagaag ttgagatggt tgctttttgt gacatcattg tagagagggc ggaaaaagcc
     8581 gctaaagagt acggcacacc ggatgcaaag gtttacgccg attacaagga actgcttaag
     8641 gacgagagtc tagatgttgt tcacgtatgt actccaaata aatctcatgc cgatataacc
     8701 attgatgcct tagaatcaga caagcatgta ttgtgtgaga aacccatggc caaaacagcg
     8761 gctgatgcca gaagaatggt agaggcggcc aaacgtaccg gcaagaaatt gaccattgga
     8821 tatcaaaaca ggttcaatcc agaatcccaa tatctccatg ggacatgccg caggggcgat
     8881 ctaggggata tctatttcgc aaaggctcat gccataagaa gacgtgctgt tcctacatgg
     8941 ggagtgttcc ttaacgaaga ggagcaaggc ggcggtccat tgatcgatat cggaacccat
     9001 gcccttgacc ttaccctttg gatggtagac aactacaagc ccaaaacagt tgttgggact
     9061 acttatagaa aattagccga gcagcgtgat acggccaatg catggggaga ctgggatccc
     9121 gaagagttta cggtagaaga ttctgccttt gggtttgttg taatggagaa cggaaccacc
     9181 ataatcctag aatccagctg ggctctcaat acccttgatg taggcgaagc gaagaccacc
     9241 ctatgcggga ccaaggccgg agcggatatg agggatggac tccgtataaa cggtgttgaa
     9301 tttaacaaga tgtataccaa aaaaccgtca ttgggtgcag gcggcgtcga tttttatgac
     9361 ggcgaagccg atgatccctc ttacttagaa gccagggcat ggatcgatgc tattataaac
     9421 gataccgatc ccgttgtatt gcctgagcag gcgctggcag taaccgagat cctagaggct
     9481 atctatgaat cagccaagac cgggaaagca atcacattct aaaaagctta ataggttctg
     9541 aaggattaac ggactatata aattctatat ttgtgtagtc cgttaattta agatgctctt
     9601 aaggcatcat acttacctta actcgttgta ctcgttcatt gccctttccc atggaggggg
     9661 taatgacctg taaaagacga gcgatttcaa tgaattcaat gtgaaaggag aaaaactatg
     9721 aaactgggtg taatgactgt tttactaggc aatatgaagg ctgaagaagc ctttaagtac
     9781 ttaagcgaat ccggcgttca ggccgttgaa ttgggtaccg gcggatatcc aggaaatgcc
     9841 cacgcaaatc ccgacgaact gctaaaagat gacggaaaaa ttgaggagct aaagaatctg
     9901 gttgataaat acaacctaga gatcagctct ttaagctgtc acggaaatcc cgttcatccc
     9961 caaaaagaga ttgcagccga attccatgga gactttgaaa agactgtatt gttggcggaa
    10021 aagctgggag taaccagggt aaataccttt tcgggctgtc ccggagattc accgaattct
    10081 atgtatccta actgggtgac atgcccttgg ccggaagact ttttaaaggt gttagattat
    10141 cagtggaatg atgtcttaat tccctattgg gaaaagactg ccgcttttgc aaaggatcac
    10201 ggggtagata tgatatgtct ggagatgcat ccgggctttg ccgtgtacaa tcccgagacc
    10261 ctcttaaaac tcagggcggc tgtcggcgat gccatcggtg cgaactttga cccaagccat
    10321 ctgttctggc agggcatcga tcctgtggcc gctatacgcg aactagaggg tgcaatatac
    10381 catttccatg ccaaggatac caagattgat gccagaaaca cggctgtcaa cggcgtgttg
    10441 gatacaaaac actatggcga cgagatcaac agatcttgga tattccgttc cgttggctac
    10501 ggtaacgact atcagatgtg gaaggacatg gtgagcacac ttagaatggt aggctacgat
    10561 tatgttctaa gtatcgagca tgaggatagt ctaatgaccc ccaacgaggg attgcaaaag
    10621 gctatagcct tcttgaaaga ggtaatggta tttgaagata aaggtgagat gtggtgggcc
    10681 taagtcctat tgaactcggc agggtatagg gtctatgatg gttttttatt acggatagac
    10741 actgcaatgt acactttcat tatttacgtg gaataggatt aaaggatcga gaacagaatt
    10801 agggacccaa acggagctaa ggggtttagg atagggttaa gggctttgta aggggaccgg
    10861 gaaatttaaa acagtaccaa tggataggga gatagaaact tttcttctcc ctatttttat
    10921 ccgtatgaaa ggacatcttc ttttcaatat ccaattcact ttactgcaca gtgggataac
    10981 gaaccagcac aggagaataa attattttcg cttttagaag gggagcgtac aggggtattg
    11041 ttatgactaa aacaaatttt acagaggctg acttgtataa acccatatat gaatatctca
    11101 cccggctagg atacacggtg cggggagagg tcaaagattg cgatgtcgtc gccattaagg
    11161 gcgaagaatt gattattata gagctgaaga agggttttag cgtggaactg ttgactcaag
    11221 ccgctgccag acagaggata accagtggtg tatacgtagc tctgccaagg cccaaggcta
    11281 gtttgagatc atccaaatgg aagggcataa gattgctcct gaggcgtatg gagctggggc
    11341 tgatctttgt caatatgaat ttggacaagc ccaaggcaga agtaatcctt cacccccttc
    11401 cctataagct gagaagggcc aagaaggcca gggagggaat aattagtgag gcggagggcc
    11461 gctccgggaa ttttaatccg ggaggcagca acaggaagaa gataatgacg gcttacaggg
    11521 aaaatgctat attcatagca tgctgcttgg aacgagacga tatattatct gcggctgaat
    11581 tgaggaaatt gggcacgggt tctaagacct attctattct ctataacaac ttctacggct
    11641 ggtttcatag ggtggccagg gggaagtatt ccctgagtga ggccggaaaa tccgccctgg
    11701 cccagtatcc ggagatagcc gatcattata gggaaaagtt atagttgtaa ctccatatcc
    11761 ccgtatttta tccagcccct ctttctcatg atttcggaaa tgactaggct gagaagagcc
    11821 ggaaggataa agtgcatcat aaggatttcc gttagaagta accaggttgg gatagtccca
    11881 tccatggtcg cccagacggc aaactggccc acaagcccac tggttcccat gccggcccct
    11941 atgggattgc cctgcatctt tagaatagtt gtagacaggg gacctaagat ggcgctggcc
    12001 aaggtagggg ggatccaaat ctgcggcctt ctgattatat tgggcacctg cagcatggag
    12061 gtgccgagac cctgggctat taagcctccc actttgttct cacggtagct tgctactgca
    12121 aagcctatca tttgggtgga acatcccacc gttgcggcac ccgcagcaag gcctgtcatc
    12181 tgcgtggaga tagccaaggc tgcgctgctg atgggagcgg tcagtatcat gcccatggaa
    12241 gtggagacca atatccccat ggggaagggc tgaagggtag tggcggtgtt tataacatgc
    12301 cccagccaaa ccataaagcc ggagatatat ggccctatga atttcccggc aagacccccg
    12361 gccagaatgg tgacggaggg gaccagtact atatcaaccc tggtctttcc ggcaattagc
    12421 ctgcccagtt cggctcctat cactgcggcc ataaaggcac ccatgggtcc ggcattcata
    12481 tagctgtatc cgaaggctcc cgtggcggca gaggagaata aaaccaaagg ttgggccttg
    12541 agcccgtggg caatggccac ccctatggcg gcgccgacta cgggggactg ggccgataga
    12601 atatctgaaa aatctgaaat aaatccgaga cccggtatcc tagagagctg ggataggatt
    12661 agtcctatta taagggatga aaaaagtccc agggccatgg cactgaaagc atctaggcca
    12721 tatcttttta gccccttttt taaaattcct tttactctgt tcatcttaac acttcctcgc
    12781 tatgtttttg tctataatgt attttgcagt attctattta attaaagcat tatgtcggtt
    12841 cattaccata ctatgcgaca aaggtaatgg gttattgaaa agcggatgac tatattttca
    12901 ataa
//
LOCUS       DASRNX010000192        11038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_410835,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000192 DASRNX010000000
VERSION     DASRNX010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11038
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410835"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1047)
                     /locus_tag="VFD89_07720"
     CDS             complement(247..1047)
                     /locus_tag="VFD89_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZJ58106.1"
                     /translation="MIKLGVNSVLFSGYDFKTAAENIALAGYDGVEISAIKGMCEHLE
                     LDKWQEQADELTTITSDHGLEFLAMEVASLDEDRLLKAFEAGAEMGIPVINVGPGGKS
                     DVEDDLKKTIDHLAKMADRAHEFGVYLCVKAHVGASIYNTPTTLKAMEAIESPGFGID
                     MDPSHIYRSGENPEDALPKVLSRVKHIHIRDCIERVGSPGTPANQACGRGNIDLFAYC
                     RAMVEGGYSGPVNLEVIGAGDLSLPQVSIIAAESYGYLNACLKILGAR"
     gene            complement(1391..2380)
                     /locus_tag="VFD89_07725"
     CDS             complement(1391..2380)
                     /locus_tag="VFD89_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ58107.1"
                     /translation="MALKVGVVGVGNIGTIHAGIYKDNPKTELVAVCDIIKEKADAAA
                     EKFGAKAFYSVKEMLDSGIHLDACSMATKGEENGGDHFTPTMELLEAGIPVLGEKPIS
                     NRIDEGEKMVELARSKKIPYAVNLNHRFTPAALLAKEWIDEDRLGRLHMINMRMWINN
                     PVETSPWFHLRALHPHSFDVIRYFAGDVKKVAAFMMKGEGRAIWSNAQVLLYFENGMI
                     GNLVGSYDAGASYGLEQCDVVGAKGRFVLEDACEHLTFYPRHSISTETYSYLGGMRSF
                     GETFNSRISDWIDQLDAGAKYDEINGSGEDALRAQYIIEAAIKSFETGQIIEL"
     gene            complement(2569..3954)
                     /locus_tag="VFD89_07730"
     CDS             complement(2569..3954)
                     /locus_tag="VFD89_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZJ58108.1"
                     /translation="MATRIKDMTDGKPARLIVTFALPLMLGSVFQQTYTIVDSIVVGR
                     VAGVEALAALGVSDWVNWMILGLVMGFAHGFTILISQRFGAEDYAGLRKTVAMCTILS
                     GIIGVAMTVVGLSLAKPILRAMQTPDNIIDNAHLYISIYSLGFPMLTAYNVSSSILRG
                     MGDSKTPLWAMIIASVINIILDIIFVVVFRLGVAGVAVATIMAQTFSFLYCLRAIKKL
                     PILRMEPGDWKLDKGIIGHLLRLSIPMACQNAIIGAGGIVVQYVINGFGFVFVAGFTS
                     TNRLYGLLEMAAMSFGFAMATFAGQNLGAGEYRRIREGVNSLVKIAIGTACAITLAAI
                     AFGRRVLMLYITGDPGEIEAVVNVAYKYLFIMAAFLFILYLLHVYRSALQGMGDTVMP
                     MVSGIVELVMRISTIILLPLLVGEIGLYFAEIAAWLGAVVVLMSAYYLRINRLENSSI
                     PREGKAMPVNL"
     gene            complement(4106..4645)
                     /locus_tag="VFD89_07735"
     CDS             complement(4106..4645)
                     /locus_tag="VFD89_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZJ58109.1"
                     /translation="MGKMALNLNNRVRMLWMQHSEWTRMAFTSVIFNNAEERQVVQRL
                     LRNPIDFACFLASFYGEGIGGRFGVLLTEHLSLAVALVRATIAQDTGNAEKIKTKLFH
                     NAEEISAFLASINPYWHYEIWKEMFFIHLDLAIEMAGQQINGEYEESINTYDRFEAEV
                     MEMARMMFQGIMKQFACAH"
     gene            complement(5169..5624)
                     /locus_tag="VFD89_07740"
     CDS             complement(5169..5624)
                     /locus_tag="VFD89_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZJ58110.1"
                     /translation="MVETQYVIFRLGEDLYGATIKDIQEIILPQSPTKVPNNPDFIEG
                     IIDYRDKVIPVLDLKKRFRLGNSEYKGQSRFVVATVDGSDVAFAVDEVTEILRAQRDE
                     IESAPDATRIGKEYIYGATHIGDRLIILLELSKVLSVDERDMLGSVGIE"
     gene            complement(5883..6566)
                     /locus_tag="VFD89_07745"
     CDS             complement(5883..6566)
                     /locus_tag="VFD89_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HZJ58111.1"
                     /translation="MNKTYKYETHMHTREGSACASATGREMVRAHIEKGYSGMIVTDH
                     FFNGNTAVPRNLPWKDRVNLFCKGYENAVDEARGTNFNVLFGWEYAFDGTEFLTYGLE
                     KKFLLDYPDILSWNVEKYFDIVHEHGGFISHAHPFREAFYIDTIRLYPEHVDAVEVIN
                     SSHTDPKFDERALAYAQKHNLLMTAGSDSHHLDGLHGGGMVFNKKINSIGDFIRAIKE
                     QTYILIKAS"
     gene            complement(6647..7748)
                     /gene="prfB"
                     /locus_tag="VFD89_07750"
     CDS             complement(join(6647..7672,7674..7748))
                     /gene="prfB"
                     /locus_tag="VFD89_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_120168821.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZJ58112.1"
                     /translation="MFELENLQETLDHINTVFTQVGDSLDIPGCQSKVKEYELQMNDP
                     SFWEDLEKSQRVNKDIKHLKNKIDRYETLKSRIEDTDILIQLGIEEEDSSVMEEAQIE
                     VGKLEKDVEALRLETLLSGPYDRNNAIISVHAGAGGTEAMDWASMLLRMYTRWCEAKG
                     YEVETLDLLPGEEAGIKSVTLGITGENAYGYLRAEKGVHRLVRISPFDSSGRRHTSFA
                     SLDVMPELDNDVELDIKPEDLKIDTYRSGGAGGQHVNKTESAVRITHLPTGIVAQCQN
                     ERSQIQNRETAMKVLKARILEQKEAEAQAEIDKMKGEMKRIEWGSQIRSYVFHPYSMA
                     KDHRTNEEMGNIQAVMDGEIDIFINSYLQMMA"
     gene            complement(7959..10661)
                     /gene="secA"
                     /locus_tag="VFD89_07755"
     CDS             complement(7959..10661)
                     /gene="secA"
                     /locus_tag="VFD89_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZJ58113.1"
                     /translation="MMGLLKRIFGDSNSREIKRIEKIVDEIEGLEPAIKALSDEQLRA
                     KTDEFKDRLKQSETLDDILTEAFAVVREASIRTLGMRHFRVQLIGGVVLHQGRIAEMK
                     TGEGKTLAATLPAYLNALTGRGVHIVTVNDYLAQRDSEWMGKIYKFLGLSVGLIIHDM
                     KSSNRRENYNADITYGTNNEFGFDYLRDNMVIYKEEMVQRELNFAIVDEVDSILVDEA
                     RTPLIISGAGEKSTDMYMKADRFVVRLRNEIDFEMDEKANTINLNEEGVAKAEAYFKV
                     EDWSNLDAAEDNPELAYLPHHVNQALKARFLMKRDTDYVVKDGQVIIVDEFTGRLMTG
                     RRYSDGLHQAIEAKEGVNVRRESKTLATITFQNYFRMYKKLSGMTGTAKTEENEFKTI
                     YGLDVVVIPTNEEMIRNDHNDVIHMSLKGKFNAVVEEIVQRYSLGQPVLVGTVTIENS
                     EILSSMLKRRGIPHEVLNAKFHEKEAEIVAQAGRYKAVTIATNMAGRGTDILLGGNPE
                     FMAKKEMKKQGYSDELLNQVSGTQKTDDPDVVAARRVYGELYNKFVMESNLEHQKVIE
                     LGGLHIIGTERHESRRIDNQLRGRAGRQGDPGASRFYISLEDDLMRLFGSDRIQGIVS
                     RLGMDDDQAIEYGLLSKQIEAAQKRVEGNNFNIRKHVLQYDDVMNTQREVIYSQRRKV
                     LEGEDLKDSIMEMVAAVIDGAVEMYVGELTSPEDWDIKGLTEHLERVFLPRGSVNISD
                     TEIFDLDKEMLKDKILEAAHKAYALQEQENGEDNMREAERVILLRVVDQKWMDHIDAM
                     DQLRQGIGLRAYGQRDPVVEYQVEGFEMFEEMIRSIQEETVHILYNIRVEARVPQRER
                     VAKPTSASHGDSGPKKPVVKGEKVGRNDPCPCGSGKKYKRCCGA"
BASE COUNT         2727 a         2930 c         2189 g         3192 t
ORIGIN      
        1 tggatggatt tgtggaggca aattccaacg gcctcaaagg aagacctcta agggaagggc
       61 cattaacact cccccaaaga cgggacccgt aaggccgctt cccgatgtag tttggaaaat
      121 gacggaataa accatcctcc ctagcaaaac agataagatg ccttaaatca ctttcatttg
      181 aaaggattcc agtttggaaa cgacaagctc ttcaatatgc agtattggac aatattgaag
      241 aggggcctat ctcgctccca atatcttaag gcaggcattt agatacccat agctctccgc
      301 tgcgattatt gaaacctggg gcagggataa atcccccgca ccgattactt ctaagttaac
      361 gggccctgaa tatccgccct caaccatagc cctgcagtag gcaaataggt caatgtttcc
      421 tctgccgcag gcttggttag ccggcgtacc ggggcttccc accctttcga tacaatccct
      481 tatatggatg tgctttaccc tgcttaatac cttaggaagg gcatcttcag ggttttcacc
      541 agaccgatat atatggcttg ggtccatatc aatgccaaat cccggggact caatagcttc
      601 catggctttt aaggtggtgg gagtgttgta gatactggcc cccacatgag cctttacaca
      661 aaggtacacc ccaaattcat gggccctgtc tgccatctta gccaggtggt ctatggtttt
      721 ctttagatca tcttcaacat ccgattttcc gcccgggcct acgtttatta cggggattcc
      781 catttcggca ccggcttcaa aggcctttag cagacgatcc tcatctaggg atgccacctc
      841 catagccaaa aattctagcc cgtgatcaga tgtgatagtc gttagttcat cagcctgctc
      901 ttgccacttg tccaattcca aatgttcaca cattcccttg atggccgaaa tctccacccc
      961 atcataaccc gccagggcga tattctctgc cgctgtttta aaatcatatc ccgaaaaaag
     1021 gacggaattg acccctaatt ttatcactaa aatccctccc atactgatag gtatatattt
     1081 ttcaattgga gaattctacg taagataggc cgaatcgtcg ttttaagggt aatgaggatc
     1141 aatcggctcc acagcaagtt cacagattct tggccttgaa acttaaatga ttgaaatgtt
     1201 attccccaaa tttatattat acacccatcg ctgatcttca tattacaaat ccgttttcat
     1261 tactgcaagc ctgctttctg ctatccaaaa tcattttttt attgaaaagc acgtaccgaa
     1321 ttccaagtcc atttcctgct gcctggaatc tttccccact gaaaaatatt tactgatgag
     1381 tataaggtcc ttagagttct atgatttgtc ctgtttcaaa ggattttatt gcagcttcga
     1441 ttatgtattg tgccctcagg gcgtcctctc ccgaaccgtt gatctcatca tatttagccc
     1501 cggcgtccaa ctggtcgatc caatcgctga tgcggctgtt gaaggtttcg ccaaaggagc
     1561 gcattccgcc caaatagcta taagtttccg tagaaatgct atgccttgga taaaaggtca
     1621 gatgctcaca ggcgtcctct aggacgaaac gccccttagc gccgactacg tcgcattgtt
     1681 ccagaccata gctagcccct gcatcataac tccctacaag gttaccgatc atcccgtttt
     1741 caaagtataa gagtacctgg gcgttggacc agatagctct gccttccccc ttcatcataa
     1801 aagcggctac ctttttaaca tcgccggcaa aatagcggat aacatcaaat gaatgcgggt
     1861 gaagggccct caggtgaaac cacggtgatg tctctaccgg attattgatc cacatcctca
     1921 tgtttatcat gtggagcctt ccaagcctat cctcgtctat ccactcttta gccaggagag
     1981 ccgccggtgt gaagcggtgg tttagattga ctgcgtaggg tatctttttg gacctggcca
     2041 gttccaccat cttctcccct tcgtcgatgc gattggagat gggcttttcc ccaaggaccg
     2101 gaatgccggc ctctagaagc tccatggtag gtgtaaagtg atcgccacca ttttcttccc
     2161 ccttggtagc catgctacag gcatctaaat gaatgccgct gtcaagcatt tccttcacgc
     2221 tataaaaggc ttttgcaccg aatttttcag cggcggcatc ggctttctcc tttatgatat
     2281 cgcagacagc caccagctca gtcttgggat tgtccttgta tatccctgca tgtatggtcc
     2341 caatatttcc caccccaact actccaactt tcaatgccat agatatgtcc tcctatccac
     2401 atatttcctg tgtatattat accataaaaa aagtaccgtt gggaatatta cggcactttc
     2461 tagaggtttc tggcgctatg ttgtaatgct gtcgttatgt tacaacctat agaagtatgg
     2521 gacatctttc cgtcttgttt taatagggaa taagatacgg tgattaccct ataagttcac
     2581 gggcatagcc ttgccttccc tgggtatact tgagttttcc agcctattta tcctgagata
     2641 gtaggcgctc ataaggacaa ctacggcgcc cagccaggca gcgatttctg caaagtagag
     2701 cccaatctcc ccaaccaaaa ggggaagcaa tattatggtg gatatacgca tgaccagttc
     2761 aactattcct gagaccatgg gcatcactgt atcgcccatt ccctggagcg cggagcggta
     2821 tacgtgtaat aaatacagga taaacaagaa cgcagccatt ataaacagat atttatatgc
     2881 tacatttact actgcctcaa tttcccccgg atcgccggta atatagagca ttaagaccct
     2941 gcggccgaat gctatggcgg ctaaggtgat agcgcaggcg gtgcctatag ctatctttac
     3001 cagagaattt acgccctctc ttatccgcct gtattcccct gcccctaaat tctgccccgc
     3061 aaaggtggcc atggcaaatc caaaggacat agcggccatc tccaagagtc catagagcct
     3121 gttggtggat gtaaagcccg ccacaaatac aaatccgaat ccgtttataa cgtattgtac
     3181 tactatgccc cccgcaccta ttatggcatt ttgacaggcc atgggaatac tgagccttaa
     3241 caggtgccca ataataccct tatcgagttt ccaatccccg ggttccattc gcaaaatagg
     3301 aagcttttta atggccctca gacaatagag aaaggaaaat gtctgtgcca taatggttgc
     3361 aacggctacg ccggccacac ccaaacgaaa taccactaca aatatgatgt ctagtataat
     3421 attgatgaca gaggcaatga tcattgccca taggggggtc ttgctgtcac ccataccccg
     3481 taatattgac gacgacacgt tataggccgt gagcatgggg aagcctaaag agtatatact
     3541 gatgtataga tgtgcattgt caattatatt atccggtgtt tgcattgccc tgagtatcgg
     3601 ttttgccagg ctgagaccta cgacggtcat cgcaacaccg ataattcccg agaggattgt
     3661 gcacatagca acagtttttc tgagtcctgc ataatcctct gccccaaaac gctgggaaat
     3721 caatatagta aagccatggg caaagcccat caccagtcct aatatcatcc agtttaccca
     3781 gtcggatacc cccagagctg ccaacgcctc taccccggct actctaccga caactatgga
     3841 atcaactatg gtatatgtct gttgaaatac gctccccagc atcaggggca gtgcaaaagt
     3901 cactattagc ctggccggtt ttccatcggt catgtccttt attctggtcg ccatctacac
     3961 attctcctat acccgcaaat aatatccggc atacaataac cggcggttac ccgcttatcc
     4021 ctgtagcgcc gggagtgctg tatggattat atcataatat tgaccgccgg ggtataggtt
     4081 gattgatgga atttttaggc ccttgttaat gagcgcaggc aaattgtttc ataatcccct
     4141 gaaacatcat ccgggccatc tccataacct ccgcctcaaa cctgtcatag gtatttatac
     4201 tctcctcgta ttccccgttt atctgctgac cggccatctc tatggccaga tccaggtgga
     4261 tgaagaacat ttccttccag atctcataat gccaataggg attgattgac gccaagaagg
     4321 ccgatatctc ttccgcatta tgaaacagtt ttgtttttat cttttccgca ttccccgtat
     4381 cctgggctat ggtagccctc accaatgcca ccgccagact taggtgttcc gtcagcaata
     4441 ccccaaacct gccccctatt ccttccccat agaagctcgc cagaaagcat gcaaagtcta
     4501 tggggtttct tagaagcctt tggactacct gcctttcctc tgcattatta aatataacgc
     4561 tggtaaaagc catcctggtc cattcgctat gctgcatcca cagcatacgg accctgttat
     4621 ttagatttaa cgccattttg cccatatgtt ccatccaaat cccctgcctt ttcccatcaa
     4681 tatgggtatt ataaaaaaca ttatccctat atgatatgcg cggggattgg ataatgtcat
     4741 gaaaaaacac cgtgcctcag ctacggtgtt atgtatcttt atatgggctg tttttgaagt
     4801 actgcctttt gatcataaat caacctgttg ttctagctaa ttgtgtcggt aatagctgct
     4861 gaactagcat aatgcgttaa attaatatga tacttttttc aatcataaag aaataagtta
     4921 aaccaaattc gtatacaata tcaattccgg actaagcctt gccaatacag tatcaggtac
     4981 acgaagattg actatgctac ctaaggttaa ctctaacgcc tagggtcaac tataatacct
     5041 gaggtcaact ataataccca aggctaacta tactacctga ggtcagttat agcaccttcg
     5101 gtcatgaaat cgtatcgaaa tgtgaccgta tcaaatgtta tacacaaaat atccttcatt
     5161 gtacatatct attcgatccc tacagaccct aacatatccc gttcatccac ggacaagacc
     5221 ttggaaagtt ccaataggat tataagccta tcgcctatat gagtcgcccc ataaatatat
     5281 tccttgccta tacgtgtcgc atcgggggca gattcgatct cgtccctttg ggccctgagt
     5341 atttctgtaa cctcatccac tgcaaaggca acatcgctgc cgtctaccgt ggctaccaca
     5401 aacctggact ggcccttata ttcgctattc cccaatcgaa aacgcttctt tagatccaag
     5461 acgggtatca ccttgtccct ataatctata attccctcta taaagtccgg gttattgggt
     5521 accttggtgg gactttgggg aagaatgatt tcttgaatat cctttatggt cgccccatac
     5581 aggtcctcac ccagcctaaa gatgacatat tgtgtttcaa ccatgaatag accccctctt
     5641 tcaccaaacg tcgcgaagtt atgataattg taccatatgg ggcatatatt gccaagatgt
     5701 ttgttttgta ggataattga gcaggataat cagacaaatt aatttgtcaa cgtatttcat
     5761 atcttaaaat actatctccc gggtatccac atctctacta tccggtccgc acatcattac
     5821 acatacattc tgctagacat tccttccgtg agattccacc agagtattcc catacgcctc
     5881 cgttaggatg ccttgattag gatataggtc tgttccttta tggcccggat aaagtccccg
     5941 atagaattga tctttttatt aaataccata ccacctccgt gaagtccatc taggtgatgg
     6001 gaatcggagc cggcggtcat cagtaaatta tgcttttggg cataggccaa tgccctttca
     6061 tcgaatttgg gatctgtatg gctggagttt attacctcca ccgcatccac gtgctcaggg
     6121 tataacctta tggtatcaat ataaaatgcc tccctaaagg ggtgggcatg ggatataaag
     6181 ccgccatgct catgcactat gtcaaagtat ttttcaacat tccaagaaag gatgtcggga
     6241 tagtccaata gaaacttttt ttccagcccg taggttaaaa attccgtccc gtcaaaggca
     6301 tattcccagc caaaaaggac attaaagttc gttcccctgg cctcatccac ggcattttcg
     6361 tagcccttac aaaaaaggtt caccctatcc ttccagggaa ggtttcttgg aacagcagta
     6421 ttcccgttaa aaaagtggtc cgttacgatc attcccgaat atcccttttc aatatgtgcc
     6481 ctgaccattt ccctgcccgt agccgaggcg caggcacttc cctccctggt atgcatatga
     6541 gtctcgtatt tatatgtctt attcaatcgt gtccccctaa ccccatatga accaaaatcc
     6601 ggaatgggcc gctattagcc cccggaccgg ttatatggat agttccttat gccatcatct
     6661 gcaggtatga gtttataaaa atatctatct ccccgtccat gacagcctgg atattgccca
     6721 tctcctcatt tgttcggtgg tccttggcca tactgtatgg atggaagacg taggacctta
     6781 tctggctgcc ccactcgatc ctcttcatct cgcccttcat cttatcgatc tctgcctggg
     6841 cctcggcctc cttctgctcc aaaattctgg cttttagaac cttcatggcc gtttccctat
     6901 tctgtatctg ggagcgttcg ttctggcatt gggccacaat gcccgtgggt aaatgggtaa
     6961 ttcttacggc ggattccgtc ttatttacat gctgtccccc tgccccgccg gacctatagg
     7021 tgtcgatctt taagtcctcc ggctttatat ccagttccac gtcgttgtcc agttccggca
     7081 tcacatccag ggatgcaaag gaggtatgcc ttcgccccga cgaatcaaag ggggagatcc
     7141 gcaccaacct atgaacccct ttttcagccc ttagatagcc ataggcattt tcgccagtta
     7201 tccctaaagt cacacttttt atgccggctt cctcgccggg caacaggtcc aaggtctcaa
     7261 cttcatagcc cttggcctcg caccacctgg tatacatgcg aaggagcatg gacgcccagt
     7321 ccatggcctc agtaccgccg gcccctgcat gtacggatat gatggcattg tttcgatcat
     7381 atgggccgct taggagggtt tccagcctta aagcctccac gtctttttcc aattttccta
     7441 cttcaatttg ggcctcctcc ataacggagg aatcctcttc ttcgatgccc agttgaatca
     7501 ggatatcggt atcctcaatc ctggatttta aggtttcgta cctgtcaatc ttatttttaa
     7561 gatgctttat gtccttgttc accctttggg atttctctaa gtcctcccaa aatgaggggt
     7621 cattcatctg tagttcatat tctttgacct tactttggca tccggggatg tcaaagtgaa
     7681 tcacctacct gggtaaaaac tgtatttatg tgatccagag tttcctggag gttttcaagt
     7741 tcaaacatat gcgtcacacc tttcgttttg aaataataaa tatctaatct aacttcgtta
     7801 atccagtata atctcttgcg atagttcgtt atctaggtaa tgagctgttg ataagcggat
     7861 ggaagtagcg ttcaatagcc attaccaata tatctaacta acaaggtcca ttaatccacc
     7921 aatggaaatg ggtttgggga tctaaaattt actatacctc aagccccgca gcacctttta
     7981 tactttttcc cgcttccaca gggacaggga tcgttccttc ccaccttctc gccctttacc
     8041 accggctttt tagggccgct gtcgccatgg cttgcactgg tgggctttgc taccctctcc
     8101 cgctggggga ctctagcctc cacccttata ttatagagga tgtgtaccgt ctcttcttgg
     8161 atgcttcgga tcatctcctc aaacatctca aaaccttcca cctgatactc tacaacggga
     8221 tccctctggc cataggccct gagtccaatc ccttggcgca gctgatccat agcatcgata
     8281 tggtccatcc atttctgatc tacgaccctt aagaggatga ccctttcggc ctccctcatg
     8341 ttgtcctcgc cgttttcctg ttcctgaagg gcatatgcct tatgggccgc ctctaaaatc
     8401 ttatccttca gcatctcctt atccaaatcg aatatctcgg tgtctgaaat attgacagag
     8461 cccctcggca aaaataccct ttcaaggtgc tctgttagcc ccttaatgtc ccagtcctca
     8521 gggcttgtga gctcgcccac gtacatctcg acagcgccgt ctataacggc tgccaccatt
     8581 tccattatgg aatccttaag gtcttcccct tccagcactt tacggcgctg ggagtatatt
     8641 acctcccttt gagtgttcat aacgtcatcg tattggagga catgctttcg gatgttgaag
     8701 ttgttcccct ctaccctctt ttgggcagct tcaatctgct tggacaagag tccgtattct
     8761 atggcctgat catcgtccat cccaagcctg cttacaatcc cctgtatcct gtcggaaccg
     8821 aagagcctca tcagatcgtc ctctaaggag atatagaacc tggaagcccc gggatctccc
     8881 tgcctgccgg cccggcccct taactggttg tctatacgcc tgctctcgtg acgttccgtg
     8941 cctattatat gaagcccacc aagttctata accttttgat gctccagatt ggattccatt
     9001 acgaacttat tgtaaagctc tccatatacc ctacgagccg ccaccacatc gggatcatcg
     9061 gttttttggg taccgcttac ctggtttagg agttcgtcgg agtagccctg ctttttcatt
     9121 tccttctttg ccatgaattc gggattaccc ccgaggagta tatccgtacc cctgccggcc
     9181 atattggtag ctatggtaac cgccttatac ctgcctgcct gggcgactat ctccgcctcc
     9241 ttctcgtgaa acttagcgtt taaaacctcg tgggggatcc ctctgcgctt tagcatggaa
     9301 cttaaaatct cagaattctc tatggtaacc gttcccacca ataccggctg tcccagggag
     9361 taacgctgaa ctatctcctc taccacggcg ttgaatttac ccttgaggct catatggatg
     9421 acatcgttat gatcatttcg gatcatctcc tcgtttgtgg gtattaccac aacgtccaac
     9481 ccgtatatgg tcttgaattc attctcctct gttttggccg tacccgtcat tccggacagc
     9541 tttttataca tcctgaaata attctggaat gtgatggtgg ccagggtctt actttcccgc
     9601 ctgacattga ccccttcctt tgcttctatg gcctggtgga gcccgtcgct ataccttcgg
     9661 cccgtcataa gcctgccggt aaattcgtcc actattatga cctgcccgtc ctttaccaca
     9721 tagtccgtat cccttttcat aagaaaccgg gcctttaatg cctgattaac gtgatgaggg
     9781 aggtatgcca gctcgggatt atcctcggca gcgtccaaat ttgaccagtc ctctacctta
     9841 aagtaggcct cggccttagc caccccttct tcatttaggt taatggtatt ggccttttca
     9901 tccatctcaa aatctatctc gttccttagc cttactacaa accggtctgc cttcatgtac
     9961 atatccgtag acttttcccc ggccccggaa atgatcaaag gagttctggc ctcgtccacc
    10021 aagatggagt ccacttcgtc aactatagcg aagtttaact ccctttgcac catctcttcc
    10081 ttatatataa ccatgttatc tcgcaggtaa tcaaacccaa attcgttgtt ggttccgtag
    10141 gtgatatccg cattatagtt ctccctgcgg ttgctggatt tcatatcatg gattatcagt
    10201 cccaccgaaa ggcccaaaaa cttatagatc ttgcccatcc attcgctgtc cctctgagca
    10261 agatagtcgt ttaccgtaac gatgtggaca cccctgcccg taagggcatt tagataggcc
    10321 ggcagggtag ctgccagggt cttaccctca ccggttttca tctcggctat ccgcccctga
    10381 tgaagcacca cgccgccgat gagctggacc ctaaagtgtc tcattcccaa ggtccttata
    10441 gaggcttctc ttaccacagc gaaggcctcg gttaagatat cgtcaagggt ctcgctttgt
    10501 tttagtcgat ctttgaattc atcggtctta gcccgaagct gctcgtcgga gagggcctta
    10561 atagctggtt ccaacccctc tatctcgtcc actatctttt ctattctctt tatctcccta
    10621 gagttactgt cgccgaatat cctttttaac aaccccataa tatatacact ccatctttca
    10681 aatattcccc ggctggatta agccccagaa cgcgactatc gggggagtat aggcagtcta
    10741 ttaaattgat atctaattct aacattaaag ccttaaacat tcaaggattt gggttttcca
    10801 tggtgcataa tctcaaaata tgttttccat ataacctcac aaataaaaga taggctcgtt
    10861 tttatatatg ttacgaaaaa gacctgccaa tagatcaatg tggttttcat aacatataaa
    10921 tcaagaaaaa tgtttttcga tatctggaac ataatgtaag atgtttatcg taacacatat
    10981 agacggataa gggtttttaa aacatgccgc caaaaataga aattcccgaa ctctgtcc
//
LOCUS       DASRNX010000193        13699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_675072,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000193 DASRNX010000000
VERSION     DASRNX010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13699
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675072"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..650
                     /locus_tag="VFD89_07760"
     CDS             36..650
                     /locus_tag="VFD89_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58114.1"
                     /translation="MFRKSRKKGYLISILVLGAMMMGGYILGTLMGRQENGENVSKKG
                     DEPGYQVVRSSDNDDLDTQGHQVPPIEVITGDTFLVFEKKYQKCGHKRVEERTAGEDE
                     IGLSIQSITLKFPEWNITEYTTERVVFVREVNDFCPGHFILKDRDGLVVIYMPTDEGD
                     VYKSVEETQISTGALPAEIQDEIRKGLVLDTLEEVEHFMENLES"
     gene            762..1583
                     /gene="proB"
                     /locus_tag="VFD89_07765"
     CDS             762..1583
                     /gene="proB"
                     /locus_tag="VFD89_07765"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HZJ58115.1"
                     /translation="MTALRKKIIDAKTIVIKVGTSTITHANGSMDFHLMEKLVRVLAD
                     LRCDGRNIVLVTSGAIGVGRFRMGMEEKPDSLPQKQALAAIGQVSLMYIYSKFFSEYD
                     QIAAQVLITRDVVDDTLKKKNTHNTFCTLLDYGVIPIVNENDTVATEEIEPYFGDNDT
                     LSAVVAELIDADLLILMSDIEGLYDKNPKCHQDAHFIPLVYDVYEQTIDGVEDTHNNL
                     GTGGMKTKIEAAKVCHGAGIPMIITNGDDPDHIYEILEGREIGTLFVPCTDFVRV"
     gene            1615..2862
                     /locus_tag="VFD89_07770"
     CDS             1615..2862
                     /locus_tag="VFD89_07770"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HZJ58116.1"
                     /translation="MEVLNKARLAKGASRELGILSSIQKDTALEMMAKALEDNIQGLI
                     AENELDVSMAVKNGRPKALIDRLVLNEERIKAMAEGLRVIKELPDPVGEVISQWKRPN
                     GMKIGQQRVPLGVVSIIYESRPNVTADAIGLCIKTGNAVVLRGSSEAIHSNKAIVEVM
                     SGAAYGAGIPKGAIGLIEDTRRESVGELVRLNGLIDVVVPRGGSGLINAVTENATVPV
                     IETGIGNCHVYIDGECDLDMAAEIAFNAKVQRPGVCNAAETLLIHEKTAPAILPGIVE
                     DLKKSDVEIRGCKRTMAIVPEILPADSGDWDTEYLDYILAVKVVDSVDDAIGHINKHG
                     SGHSEAIVTSNYYNAEKFLDQVDAAAVYVNVSTRFTDGFEFGFGAELGISTQKLHARG
                     PMGLKELTTTKYIIRGSGQIRGN"
     gene            2946..3449
                     /gene="ruvC"
                     /locus_tag="VFD89_07775"
     CDS             2946..3449
                     /gene="ruvC"
                     /locus_tag="VFD89_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZJ58117.1"
                     /translation="MVILGIDPGLAIVGYGVIESRNNALKAIDYGVITTPSTLEMPKR
                     LVSIYDGVHELITRYQPDEVAVEELFFNKNVKTALTVGHARGVAVLAAAKTGLDLYEY
                     TPLQVKQSVVGYGRAEKSQVQQMVKLLLSLRDIPKPDDAADALAVAICHSHSANMGRR
                     FGGRAFY"
     gene            3574..4170
                     /gene="ruvA"
                     /locus_tag="VFD89_07780"
     CDS             3574..4170
                     /gene="ruvA"
                     /locus_tag="VFD89_07780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZJ58118.1"
                     /translation="MFAYIKGKLAEIHPQKVVLEVGGIGYEIKVSESIMSKLPGKGNQ
                     IKLHTYFNVREDTQELYGFFDMEEKSIFEKLITVSGIGPKVSMAILSVLTPTQLALAI
                     VTDDIKTLCTAPGVGKKTAQRMVLELREKIDKEALMGRGEADFEDVAVDGHNEVIEAL
                     MALGYQPQDARFAFNAIEDKDQDVSTLIRLSLKSMDRR"
     gene            4217..5236
                     /gene="ruvB"
                     /locus_tag="VFD89_07785"
     CDS             4217..5236
                     /gene="ruvB"
                     /locus_tag="VFD89_07785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZJ58119.1"
                     /translation="MQEEDRIITPTMVETDREIEQSLRPRTIDEYIGQEKLKEKLDIF
                     IQATKQRSEALDHVLLYGPPGLGKTTLAGIIANELGSGIRITSGPTIERPGDLAAILT
                     NLGEADVLFIDEIHRLNRTVEEILYPAMEDFALDIIIGKGPSARSIRLDLPKFTLIGA
                     TTRAGMLTSPLRDRFGIINRMELYNQDELKLIVTRSAGILDIAIEEDGAVEIAKRSRG
                     TPRVANRLLKRVRDYAQIKADGIITYEVACEALDLFEVDSIGLDEVDRKILLTMIEKF
                     VGGPVGVDTLAASTGEETITIEDVYEPYLLQLGFIARTPRGRIVTPLGYKHMGIPHEE
                     QTTLV"
     gene            5332..6357
                     /gene="queA"
                     /locus_tag="VFD89_07790"
     CDS             5332..6357
                     /gene="queA"
                     /locus_tag="VFD89_07790"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZJ58120.1"
                     /translation="MKVLDFDYDLPEELIAQHPIPIRDQSRLLVYHRQYKKTEDRLFS
                     HIKEYLRPGDCLVINDTRVIPARLYGVREDTGGRVEILLLEKGISNEWQALVRPGRRV
                     RVGSNLSFGDGRLKARVMDILADGSRVMRFYFKGIFEEILDELGVMPLPPYIHERLDE
                     GERYQTVYNRYAGSSAAPTAGLHFTDELLDDIENIGVKIARITLHVGPGTFRPVKVNN
                     IREHKMHFESYSVSEDAAEIINRCRSNGGRIIAVGTTSTRTLETVADEEGIVYPGSGK
                     TDIFIYPGYAFKGIDGLITNFHLPKSTLIMLVGALIGKDEVLGVYKKAVEARYRFFSF
                     GDAMLIL"
     gene            6445..7563
                     /gene="tgt"
                     /locus_tag="VFD89_07795"
     CDS             6445..7563
                     /gene="tgt"
                     /locus_tag="VFD89_07795"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZJ58121.1"
                     /translation="MAFIFKFELIKTCSETGARLGKLYTPHGVIDTPVFMPVGTQATV
                     KATTPKVLDELGAEIILSNTYHLYLRPGHDLIHDAGGLHGFMNWNKPILTDSGGFQVF
                     SLADLRRISDDGVEFQSHHDGSLHFFSPEKVMEIENKLGADIIMAFDICSPYPCDYED
                     AKKDLSRTLKWAKRCKEAHHREDQMLFGIIQGGMYEDLRLKSAEEMLAIDFPGYGIGG
                     LSVGEPKGLMYEVLEALDPVMPKNKPRYLMGVGSPDCLVEGVLRGIDMFDCVLPTRIA
                     RNGTAMTSQGRLVVRNAAYQRDFTPIDPKCDCYTCRNFTRAYIRHLVKAGEMLSSILI
                     TIHNIRFLINLMKDMRQAILEDRLLEFRESFFDEYGRF"
     gene            7830..8144
                     /gene="yajC"
                     /locus_tag="VFD89_07800"
     CDS             7830..8144
                     /gene="yajC"
                     /locus_tag="VFD89_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZJ58122.1"
                     /translation="MLLTPIVNPQEGGLGAILMSLLPIILLIAVFYFMIIRPQRKKDK
                     ENREMLASLTVGDNITTIGGICGKITSIREDIVIVEVGADKVKLVFERWAIRNVDKIT
                     AD"
     gene            8323..8730
                     /locus_tag="VFD89_07805"
     CDS             8323..8730
                     /locus_tag="VFD89_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HZJ58123.1"
                     /translation="MRKMRKAPNKQQPESIRTDILAILKSSIIAIIITLICFTFFAII
                     IKVADLQETIIPPVVQVIRTLSIAFGGMLAAKSSKKVGWLKGGITGVVYVLLAFIISS
                     LFGGSIFMGTLIFSDILLGAIAGAVGGIIGVNI"
     gene            9178..10422
                     /gene="secD"
                     /locus_tag="VFD89_07810"
     CDS             9178..10422
                     /gene="secD"
                     /locus_tag="VFD89_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZJ58124.1"
                     /translation="MKNKRILYLVLIFSFILGISLVSYVALKGLSLWGPYEIPPIGQQ
                     VRQGLDLKGGLYVVYEAKMEPGEPDKEDKISGAIRVIRNRLDKEGQHEATIAKQGENR
                     IQVEIPGIKNPRELAEILAKPAVLEFEGPEGDIIITGKDIKSAKPAYGEGHTPVVDFE
                     LHAEGTKKFADATTEYKGQIIRILLDKNPISEPRVKTAIVGGKGIIDGMKDIEEAKRL
                     ANLIESGALPVNLEQAETRTIGATLGVNALYRAIQAGIIGVVGVLLFMLIFYRLPGLV
                     ADLALTVYMFILMTVIVSAKVTLTLPGIAGIILSVGMAVDANVIIFERIKEEIRSGKT
                     LNSSVDSGFSKAFRAILDSNITTLIAAVVLLYFGTGPIQGFAKTLIIGILASMFTAIS
                     FTRFILKLVMGLNIKNKWLYGA"
     gene            10436..11326
                     /gene="secF"
                     /locus_tag="VFD89_07815"
     CDS             10436..11326
                     /gene="secF"
                     /locus_tag="VFD89_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZJ58125.1"
                     /translation="MKFHVVKHIKLWIAIPLAIILIGFGLMAFRGFNLGIDFIGGSVI
                     HAGMDTDYDVNDVRDIINKLGINADIVSAGQNNQDIIIRLRYMEGQQEIHDKIIAGLQ
                     ERYNLDEEKFIIEFVGPTMGRELIRDAVTSVLIASGLILVYIWIRFELKSGFAAIIAL
                     IHDILVMIAVTSIVGIQVNSSFIAAILTILGYSINDTIVIFDRVRENAKRLGRKMDMQ
                     EIVDQSINDSIIRTLNTSLTTIFAVAALYILGVESIRDFTLPILVGLISGTYSSIFIA
                     SPIWAVWDKGPGHRRTATAK"
     gene            11389..>13699
                     /gene="recJ"
                     /locus_tag="VFD89_07820"
     CDS             11389..>13699
                     /gene="recJ"
                     /locus_tag="VFD89_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZJ58126.1"
                     /translation="MAILELIKSPLEQTDRAIHRLQRALDISKVTSRVLVNRGIYDEE
                     HAKIFLNPNLEQLFDPFELCDMDKAVHRINEAADKREHITIFGDYDVDGITSSAILYD
                     LLTDLGCKVDVYIPNRHREGYGVNLEAIGAICNKGSTLMITVDCGITSVDEVEFAGNK
                     GMDVIITDHHQCGPILPEAIAIINPSRNPNLHEKHPLAGVGVVGKLVQAIGGQSYLKR
                     YLDLIALGTVADVVPVIGDNRVFVAEGLIKMNRDPCIGIEALMSISGLGDKDINTGRI
                     AFGLAPRLNAAGRIDDPLAGYRLLSAKRLSDAIPIARLLEEQNRQRQAMEAQIIQDAQ
                     EMMETQVDLGSDKIIVLGKEGWNSGVIGIAASKIVEKYCRPCILIAIKDGLGVGSARG
                     IGGFSIYDAIDSCSHLLKKFGGHHQAAGISLAEGKIPDLRSGLLEYCDKAIRDDMLIP
                     RHKYDAELGPEDISYSLIDELQALQPFGIGNPSPTFLVPEAYLAEYARVGGQGNHLKV
                     SVDLGKRLWDGIAFGMGERSEFLSTVNKVSIVTGLDRNEWRGVSKIQFNIKHMDMVLE
                     CRNDWTNFLAFFHLKFFDAFFRDFMYNKHCNYAIFPQQPGMASRKTIEEVFSELKQNR
                     MGNLILINSLWISQELIGLILDSPLLDRIEYSYGQSYNWDGIGVNRLVFAPIYSELDF
                     SGYRNVYLFEGEISCCLIDPNDINSEIYTIAGELGRADQDSRLREEYLVEHRDFALFY
                     RWIRSLSPGRNIWRDWLELVNSYRELDPCI"
BASE COUNT         4010 a         2487 c         3599 g         3603 t
ORIGIN      
        1 cactataacc caaatatgag tccggagtga ttatagtgtt tcgcaaaagt aggaaaaagg
       61 gttatttgat ctctatactt gttttaggcg ccatgatgat ggggggttac atcctgggga
      121 cccttatggg aagacaggaa aatggggaaa atgtaagcaa aaaaggggat gaaccggggt
      181 atcaggttgt tcggtcgtca gacaacgatg atttggacac ccagggtcat caagtaccgc
      241 caatagaggt tattaccggg gacacatttt tggtattcga aaagaaatac caaaaatgtg
      301 ggcacaaacg ggtagaggaa agaacagcag gcgaggatga gattggtctg agcatacaaa
      361 gcatcacgct aaagttccct gaatggaata ttaccgaata cacgacagag agggttgtgt
      421 ttgtaaggga agtcaatgat ttttgtccgg gccatttcat acttaaggac agggacggat
      481 tggtggttat ttacatgcca accgatgaag gtgatgtata taagagcgtt gaagagaccc
      541 agatatccac cggcgcacta cctgcggaga tacaagatga aatacgaaag ggcctggttt
      601 tagacaccct cgaggaagtt gaacatttta tggaaaacct ggagagttag cccgatttgt
      661 tgtcttaccc ccttagtcaa ctcagtaagg gggttttcta ttgccattta tgagagaaaa
      721 gggtaaaata ctatcaatga cctaataggg gtgaaggtaa tatgactgcg cttagaaaaa
      781 aaattattga tgccaaaact atcgtgataa aggtgggcac atcgactatt acccatgcaa
      841 acgggagtat ggacttccac ctcatggaaa aactggtaag ggtattggca gacctgagat
      901 gcgatggaag aaacattgta ctggttacat ctggcgccat cggtgtcggt cgttttcgta
      961 tgggtatgga ggaaaaaccc gactcgctgc cgcaaaagca agccttggca gccattggtc
     1021 aggtcagcct tatgtatatt tattccaaat tctttagtga gtacgatcag attgcggccc
     1081 aggtgcttat aacccgagac gttgtagatg atacccttaa aaagaaaaac acccacaata
     1141 cgttctgtac ccttctcgat tatggggtca taccaattgt taacgagaat gataccgttg
     1201 ccaccgaaga gatagaaccc tattttgggg ataatgatac cctatctgcc gtggtagccg
     1261 aacttataga tgctgatctc ttgattttga tgtcggatat agagggatta tatgataaaa
     1321 accccaaatg tcaccaggat gcgcatttca ttccccttgt ttacgatgta tatgagcaaa
     1381 ctattgacgg agtagaagat acccataaca atctcggtac cggtggaatg aagacaaaga
     1441 tagaggccgc aaaagtttgt cacggagcag gcataccgat gatcatcacc aacggggatg
     1501 atcccgatca tatatatgag atactggaag gcagggaaat aggcacccta ttcgttccct
     1561 gcacggattt tgttcgagtt taaatagagt gtaaagggaa aggggcgaaa gcaaatggaa
     1621 gtgttaaata aggcgagact ggctaagggg gccagcaggg aattggggat tctctcatcg
     1681 attcaaaagg atacggcctt ggagatgatg gccaaggcct tggaagataa catacagggg
     1741 ctaatagccg agaatgaatt agatgtgagc atggccgtaa agaatggccg gcctaaagcc
     1801 ttgatagacc gcttggtcct taatgaagaa aggataaaag cgatggctga ggggcttagg
     1861 gtgataaaag agctgccgga tcccgtgggc gaggtaattt cccaatggaa aagacccaat
     1921 ggtatgaaaa tagggcagca aagggtcccg ctaggtgtgg tctctataat atatgaatcc
     1981 cgccccaatg taacggcaga tgctattggt ctatgcatta agaccggtaa tgccgttgtt
     2041 ttaaggggaa gttccgaagc tatccattcc aataaggcta tcgtagaggt aatgagcggg
     2101 gcagcttatg gagcggggat tcccaaaggg gctatcgggc tcattgagga tacccgaagg
     2161 gaaagtgttg gggaattggt caggctaaac ggattaatcg acgttgttgt gcccaggggc
     2221 ggttcagggc tcattaatgc tgtaacggag aacgctacgg ttcccgtcat agaaaccggg
     2281 atagggaatt gccacgttta tatagacggt gaatgtgacc ttgatatggc agctgaaatt
     2341 gcatttaacg ccaaagttca aaggccgggg gtatgtaacg ctgcggagac actattgatc
     2401 catgaaaaga cagctccggc cattttaccc ggaatcgtcg aagatctcaa gaaaagcgac
     2461 gttgaaatac ggggctgtaa acgaactatg gctattgttc ccgaaatttt accggccgac
     2521 tcaggtgatt gggataccga atatttagat tacatccttg ccgttaaggt ggtggatagc
     2581 gttgatgatg ccataggcca tataaataaa catgggagtg gccactctga ggctatcgtc
     2641 acttcaaact attataatgc agagaagttt ctggaccaag tcgatgcagc agcggtgtac
     2701 gtcaacgtat cgacccgatt cacagatggt ttcgaatttg gatttggtgc agaattgggc
     2761 atcagcacac agaagcttca tgctaggggt cctatggggc tcaaggagtt gaccactacc
     2821 aaatatataa taaggggaag cggccagata aggggaaatt gattgattcc ctaaggcatt
     2881 taaagtctta gtaagaaaag agtgttggag aatcaaaata gagggattta ggatgtggat
     2941 attacatggt gattcttggg atcgatccag gtttggcaat agtgggatat ggggttatag
     3001 agagccgcaa taatgcattg aaggctatag attatggggt gattactacc ccttccacct
     3061 tagagatgcc aaagaggtta gtatccatat atgatggggt acacgagctt atcacccgat
     3121 atcaaccgga tgaggtagcg gtagaagagc tatttttcaa taaaaacgtt aagacagctc
     3181 tgacagtagg tcatgccagg ggggtggcgg tactggcagc agcaaagacc ggactggatc
     3241 tctatgaata tacgcccctt caggttaagc aatccgtagt tggctacggt agggctgaga
     3301 aaagtcaggt ccagcagatg gtgaagttgt tattgagcct cagggatata ccgaagcccg
     3361 atgatgcagc tgatgccttg gccgtagcta tatgccatag ccatagtgcg aatatgggca
     3421 gaaggtttgg ggggcgggca ttttattgag gtacttatgg acaaaatcaa cagttacatg
     3481 tcagacccta tcagacttta gtttggactg gccggtagat cgcagtgata taaaaagatc
     3541 gcaaaataga atgctcgttg aaaggagcgt agaatgtttg cttatataaa aggaaagctg
     3601 gcagaaatac atccccagaa ggtggtatta gaagttggcg gaatcgggta tgagattaag
     3661 gttagcgaat ccattatgtc caagctcccc gggaagggaa atcaaataaa attacacaca
     3721 tatttcaacg ttcgggagga tacccaagaa ctatatggtt tttttgatat ggaggaaaag
     3781 tccatattcg aaaaactcat cacggtatcg gggattggac ctaaggtatc tatggccatt
     3841 ttatcggtgc tgactccgac acagctggct ttggctattg ttacggacga tatcaaaacc
     3901 ctgtgtaccg cacccggggt cgggaaaaag actgcacaaa gaatggtatt ggagcttagg
     3961 gaaaaaatcg acaaggaagc attgatggga cggggggaag cggacttcga ggatgttgcc
     4021 gtcgatggac ataatgaagt tattgaggcc cttatggcat tgggatatca gccccaggat
     4081 gcgaggtttg cgtttaatgc catcgaagat aaagaccagg atgtctccac tttgatcaga
     4141 ttatccctaa agtcgatgga taggcgttag agaagggaac ggttacagaa cggacattga
     4201 tgggggtgaa tcattaatgc aagaggaaga ccgtataatt acaccaacca tggtggaaac
     4261 agacagggaa atagagcaga gtttgaggcc caggactata gatgaataca tcggtcaaga
     4321 aaagctaaag gaaaaactcg atatttttat tcaggctacg aagcagcgct ccgaagcctt
     4381 ggaccatgta ttgttatatg gaccgccggg actggggaag accaccttag ccggtataat
     4441 cgcaaacgaa ctgggctccg gaataaggat tacttcgggc cctaccatag aaaggcccgg
     4501 ggatctcgcc gctatattga ccaacttagg tgaggcggac gtgttgttta ttgatgagat
     4561 ccatcgtctg aacagaactg tagaggagat tttatatccg gcaatggagg attttgccct
     4621 ggatattatt attgggaaag ggcccagtgc cagatccata aggctggatc tgcctaagtt
     4681 tactcttatt ggagctacta ctcgtgccgg catgctgacc tcccccctga gggataggtt
     4741 tggtattatc aataggatgg aattatataa tcaagatgaa ttaaagctca ttgtaaccag
     4801 atctgccggg atattggata tagctataga agaggacggt gcagtagaga tagccaagcg
     4861 gtccagggga accccaaggg ttgctaatcg cctgttaaag cgggttaggg actatgccca
     4921 gatcaaagcg gatggcataa tcacctatga ggtagcttgt gaggccctgg atttgttcga
     4981 agtggattcc ataggattgg atgaggtgga caggaagata ttgctaacca tgattgaaaa
     5041 gtttgtcggg ggacccgtgg gtgtagacac cttggctgct tcaacaggag aggagactat
     5101 taccatagag gatgtatatg aaccgtatct gctgcaatta ggttttatag ccagaacacc
     5161 caggggtagg atagttacgc cgctgggata caagcacatg ggtatccccc acgaagagca
     5221 gacgactctg gtataaagcc gatataatga caccgggcac cccatagcca ttaccggcat
     5281 atttaattgg cgcgatgcgt tgctttacta tcttacaaaa ggagaaatga cttgaaggta
     5341 ttggactttg attatgacct accggaggaa ttgatcgccc agcatcctat ccccataagg
     5401 gatcagtcaa ggttactggt ctatcataga caatataaaa aaacagaaga tcggcttttc
     5461 tcacacataa aggaatattt aagacccggg gattgccttg taattaacga tacgagggtt
     5521 atacccgcca ggctatatgg tgtgcgggag gacacaggcg gcagggtgga gatactatta
     5581 ttggaaaagg gaataagcaa tgagtggcag gccctggtca ggcccggcag aagggtaagg
     5641 gtcggttcca atttatcctt tggtgatgga cgattgaagg ctagggtcat ggatatcctt
     5701 gcggacgggt cccgggttat gaggttttac tttaaaggta tttttgaaga gatattggat
     5761 gaattagggg ttatgccgtt gcccccctat attcatgaaa ggcttgatga aggggaaaga
     5821 tatcaaacgg tatataatag gtatgccgga tcatctgcag cacccaccgc aggtctgcat
     5881 tttaccgatg aactcctgga cgacattgaa aatattggag taaagatagc aagaatcact
     5941 ctacatgtgg gcccgggaac ctttagaccg gttaaagtga ataatatccg ggaacataaa
     6001 atgcatttcg aatcctattc tgtatccgag gatgcggctg agatcatcaa tcgatgtcgg
     6061 agtaacggag gcaggattat agcagtgggc actacctcta cccgtacctt ggaaaccgtt
     6121 gctgacgagg aaggcattgt atatcccggg agcggtaaga cagatatctt tatctatccg
     6181 ggttatgcct ttaaaggtat tgacgggctt ataaccaatt ttcacctgcc aaagtctaca
     6241 ttgataatgt tggtaggagc cttgatcggc aaagacgaag tcttgggtgt atacaaaaaa
     6301 gccgtagagg cgaggtacag attttttagc ttcggtgatg cgatgctgat tctgtaacta
     6361 tagagtgatc aggctacaga taggaactat gtatggatcg ggaatccggg aattaaaaac
     6421 gattttgcag gaatttcatc ggaggtggct tttatattta aatttgaact tataaagacc
     6481 tgttctgaaa caggagcacg ccttgggaaa ctctatactc cccacggagt tatagacacc
     6541 cccgtcttca tgcctgtggg tacgcaggcc actgtgaagg caacaacccc gaaggttttg
     6601 gacgaattag gcgctgaaat tatattatca aacacttacc atctgtatct gaggccggga
     6661 catgatctta tccatgatgc aggcgggctt catggattta tgaactggaa taagcctatc
     6721 cttacggaca gcggggggtt tcaggtattt agtctggcag atttaaggag gatcagcgat
     6781 gacggggtag aatttcaatc ccatcatgat gggtcccttc acttttttag ccccgaaaag
     6841 gtcatggaga tagagaataa attaggggcg gatatcataa tggcattcga tatctgttca
     6901 ccctacccct gtgactatga ggatgccaaa aaggatctga gcaggacatt aaagtgggct
     6961 aaaagatgta aagaggcaca tcaccgggaa gatcagatgt tgtttggaat aatccagggc
     7021 gggatgtatg aggatctgcg gctcaaaagt gccgaagaga tgctggcaat agatttccca
     7081 ggttatggca tagggggtct gagcgtaggg gagcccaagg gtcttatgta tgaggtacta
     7141 gaggcattag atcctgttat gcctaagaat aagccaagat atttaatggg cgtaggatca
     7201 ccggattgtt tagtggaagg ggtacttagg ggaatagata tgttcgattg tgtcttgcct
     7261 acccgaatag cgcggaatgg gaccgctatg accagccagg gaaggttggt agtgcgaaat
     7321 gcggcttatc aaagggattt tacgcctata gatccgaagt gcgattgcta tacgtgcaga
     7381 aattttacca gggcttacat ccggcacttg gtaaaggcgg gagagatgct ctcctccatt
     7441 ctaattacta tacataatat aagattttta ataaacctga tgaaggacat gcgccaggct
     7501 atactagaag acagacttct cgagtttagg gaaagttttt ttgatgagta cggcagattc
     7561 tagggctgtc aacaggtatg agagcctatg gcgtttaatt gcaataccta tgcctgaaaa
     7621 tttaggcttt gctacattca ggataataat gtataattta aaaaggacca gttatagata
     7681 aacaatacgc aaactaaatt tattggcacg acaattggaa tggttttata caatttcgtg
     7741 ttaataaatt tcatagctgg agttgtttat caacaaatat ccatgaacat gatgattagg
     7801 aaaaagctgt atgaaaggaa gggatgaaga tgcttctaac acctattgtt aatccacaag
     7861 aaggtggcct gggcgctatc ttaatgagcc tgttacccat tattttgcta atagccgttt
     7921 tctattttat gattatacgg ccgcaaagaa aaaaggataa ggaaaacagg gaaatgttgg
     7981 cttcattgac tgtgggggac aacatcacta ccattggagg gatttgtggc aaaatcacct
     8041 ctataaggga ggacatagta atagttgagg taggcgcaga caaggttaaa ctcgtgtttg
     8101 aacgctgggc cattagaaat gtagataaga ttaccgccga ttaaaaagga gctcgttcta
     8161 aaaaaagaac gggctttttt aatacaaccg ttcaaacctt gacatctgct gtaatgggaa
     8221 tatataggcc ggggatcgca taatttagaa ttgaaggtct ttcaaataat aaagcggaag
     8281 tgttaattca gccattacaa ttattttaag gggatggatt gaatgagaaa aatgcgtaag
     8341 gcgcccaata aacagcaacc cgaatccatt aggacggata ttttggcgat acttaaaagc
     8401 tcgattattg ctataattat cacattgatt tgttttacat tcttcgctat aatcatcaaa
     8461 gtggcggatt tacaggaaac gataatcccg cctgtggtcc aggtgattcg caccttgagc
     8521 atagcttttg gcggaatgct ggctgctaaa tccagtaaaa aggtagggtg gcttaaaggc
     8581 ggtataaccg gggtggtata tgttctgtta gcttttatta tcagctccct gttcggcggt
     8641 tccatattta tgggcacgct tatattctct gatatactct tgggggcgat tgccggtgcg
     8701 gtgggcggta ttattggggt gaacatttaa gtttttcggt caataaattc gggaggctgt
     8761 tgggagtgtc tgtcctacgg acgcacccat acggtcacgg ggcgtattaa ttcgccataa
     8821 atccggccgt actatatgac ccacatccgg gtggatgcta aaagggctag ataatttatt
     8881 acgccctttt ttaagctgta aaaaagtcaa ctggggtata tcatcggatt ttcagaagct
     8941 tcccaaatgc tcgttcgcta ttgacgggag ttctccaaag taatataatt gttgaagtgg
     9001 gcatcattat aggtaatgag aagtttttta tgtgtaggga gatgtggatt ctcaaaagcg
     9061 tatctttccc ttataggtat ttgacaatgg tttaaatatc ttaaaaatga tcatacaaat
     9121 tttcgttccc ggataagtcc cataataatt caatggatga tagtaggggg atatgatatg
     9181 aagaataagc gtattttata tctggttctc attttttcat ttattttagg catttcactg
     9241 gtctcctatg tagcgctgaa ggggctatcc ctatggggac cctatgagat tccgcccata
     9301 ggacaacagg tccgtcaggg attggattta aaagggggcc tgtatgtagt ctatgaagcg
     9361 aagatggaac ctggagagcc ggacaaagag gataaaatct ccggtgccat aagagttata
     9421 agaaataggc tggataagga aggacagcat gaggctacca tcgccaagca gggggagaac
     9481 cgaattcaag tggagatccc ggggataaaa aacccaaggg agcttgcgga aattttggca
     9541 aaacccgctg ttttagaatt tgagggtcct gaaggtgata ttataatcac cggtaaggat
     9601 attaaatctg caaaacctgc ctatggggaa ggacatactc ccgtggtaga ttttgaactg
     9661 catgcagaag ggactaagaa atttgccgat gcgaccaccg agtataaggg acagataatt
     9721 agaattttat tggataagaa ccctatatcg gagcctaggg tgaaaactgc cattgtaggc
     9781 ggaaaaggca ttatcgacgg catgaaggat atagaagagg caaagcgttt ggcaaatctt
     9841 atcgaaagcg gtgccctacc ggtaaattta gagcaggcgg agacccgtac catcggagct
     9901 accttgggcg tcaacgcttt atacagggct attcaagccg ggattatagg tgtagtgggc
     9961 gtgttgttgt ttatgttgat tttttacagg cttccgggat tagtggcaga cctcgcgctg
    10021 acggtgtata tgttcatctt gatgacagtg atcgtttctg ccaaagtaac cttaaccctt
    10081 ccgggcatag cgggaattat tttgtccgtg ggtatggctg tggacgccaa cgttattatc
    10141 tttgagagga ttaaggagga aatccgttcc ggcaaaacct taaattcatc ggttgattca
    10201 ggtttttcca aggcctttag agccatattg gattccaata taactacatt aatagctgct
    10261 gttgtcctgc tttattttgg gacaggccct atacaggggt ttgctaaaac gctcataata
    10321 ggtattttag ccagcatgtt cacggctatt tcctttacgc gttttatttt gaagctggtt
    10381 atggggctga acataaagaa caaatggctc tatggtgcat aagggaggaa aaattatgaa
    10441 atttcatgtt gtaaaacata taaagctatg gatagccatt cccctggcaa taatcctgat
    10501 cggattcggg ttaatggcct ttaggggctt caatctggga atagatttta taggcggaag
    10561 tgttatccat gccggcatgg acacggatta tgacgtaaat gatgtacggg atatcataaa
    10621 taaattgggg atcaacgctg atatcgtaag tgccggccaa aataatcagg atattataat
    10681 caggttaagg tatatggagg gccagcagga gattcacgat aaaataattg cagggcttca
    10741 ggagagatac aatttagatg aggagaaatt catcatagag tttgtcggcc ccaccatggg
    10801 tagggaatta atccgagatg cggtcacatc cgtcctgata gccagtgggc tgatccttgt
    10861 atatatatgg attagatttg aattgaaatc cggctttgct gctataatcg cgctgattca
    10921 tgatatattg gttatgatag cggtaacgtc cattgtggga atacaggtca atagctcctt
    10981 tattgcggca atactaacta ttctcggata ttccataaac gataccatcg ttatttttga
    11041 cagggtcaga gagaatgcca agcgcctggg aaggaagatg gatatgcaag agatagtgga
    11101 ccagagcatc aacgatagca ttatcaggac cctcaacacc tccctgacca caatatttgc
    11161 cgtcgctgct ctctatattc ttggagttga atccataagg gattttacac ttcctatttt
    11221 ggtaggcctt atttccggga cctactcgtc aatatttata gccagtccca tctgggcggt
    11281 ctgggacaaa ggaccgggtc atcgtaggac ggcaacggca aaatagtttg agtgtaaaag
    11341 cttccacttg gaagctttta attttattac gggttaaagg tggagtttat ggctatttta
    11401 gaactgataa aatccccgct ggagcagaca gacagggcaa ttcatcgcct gcaaagagcg
    11461 ttggatatat ccaaagtaac ctctagggta ttggttaacc ggggaattta tgacgaagag
    11521 cacgcaaaaa tatttctaaa tcctaatttg gaacaattat tcgacccatt tgaactatgc
    11581 gatatggaca aggcagtcca taggataaat gaggcagcag acaaaaggga acatattact
    11641 atctttgggg actatgatgt agatggaata accagctctg ctatcctgta tgatttgctg
    11701 acggatctgg gatgtaaggt ggatgtctac attccaaatc ggcatcgaga agggtatgga
    11761 gtcaatttag aggcaatagg cgcaatctgc aataagggtt caactctaat gatcaccgtc
    11821 gattgcggta taacctccgt cgatgaagtc gagtttgctg gtaacaaggg catggacgtt
    11881 attattacgg atcaccacca gtgcgggcct atccttcctg aggccattgc cataatcaac
    11941 ccgtcccgaa atccaaacct tcacgaaaag catcccctgg caggggtagg ggtagtaggc
    12001 aaactggtgc aggccatagg cggacaatca tatctcaaga ggtatttaga tcttatagcc
    12061 ctcggcactg tggctgacgt tgtcccggta atcggggata acagggtgtt tgtagcagaa
    12121 ggtcttatca aaatgaacag ggatccgtgt atagggatcg aggcccttat gagcatttca
    12181 ggacttggag acaaggatat aaatacgggg agaattgcat tcggtttggc acccagatta
    12241 aatgcggccg gtaggataga tgaccctttg gcgggatata gacttctcag tgccaagaga
    12301 ttatccgatg cgatccccat agccagactt ttagaagagc aaaacagaca aagacaggct
    12361 atggaagcac aaattataca ggatgcgcag gagatgatgg aaacccaggt agatctaggt
    12421 agtgataaaa taatagtatt gggtaaggaa ggctggaatt ccggagttat agggatcgct
    12481 gcatccaaga ttgtggaaaa atactgtagg ccttgtatac ttattgcaat caaagacggt
    12541 ttaggggttg gatcagccag ggggattggt gggtttagca tatatgatgc tattgattca
    12601 tgttcgcacc tgcttaaaaa atttggcggc caccatcagg ctgcaggtat ttctttggct
    12661 gaggggaaaa tacccgattt gcggagtgga ctcttagagt attgtgataa agcaattcgg
    12721 gatgatatgc tgataccgag acataaatac gatgcggaat tgggccctga agacatcagt
    12781 tacagcctga tagatgagct ccaggcgttg caaccttttg gtatagggaa cccttcgccg
    12841 acatttttag taccggaggc atatctggca gagtatgcac gggtgggggg acaaggcaat
    12901 catcttaaag tgtctgttga tttggggaaa agactttggg acggaattgc ttttggaatg
    12961 ggtgagcgct ccgaatttct atcgaccgtt aataaggtgt caattgttac gggattggat
    13021 aggaatgagt ggaggggagt cagcaaaatt caatttaata taaagcatat ggatatggta
    13081 ttggagtgcc gtaacgattg gacaaacttt ctggctttct ttcatttgaa attttttgat
    13141 gcatttttcc gtgattttat gtataataaa cattgtaatt atgctatttt tccccaacaa
    13201 ccgggaatgg catcgcgaaa gaccatagaa gaggtctttt cggaattaaa acagaataga
    13261 atgggaaatt tgatcctgat taattcttta tggattagtc aggagcttat agggcttatt
    13321 ttagatagcc ctcttttgga taggatagaa tacagctatg gtcaatccta taattgggat
    13381 ggtattgggg taaacaggtt ggtctttgcc ccaatataca gcgaattgga tttttccggc
    13441 tataggaatg tttacctttt tgagggtgaa atttcttgct gtcttattga tccaaatgat
    13501 attaacagtg aaatatacac catagccggc gaactggggc gggctgacca agactcccgg
    13561 ctacgggaag aatacttggt ggagcacaga gattttgctt tattttacag gtggatacgt
    13621 tccctttccc ccggacgcaa tatttggcgg gattggttgg aattggtgaa tagctataga
    13681 gaattggatc cttgtataa
//
LOCUS       DASRNX010000194         5219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_516337,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000194 DASRNX010000000
VERSION     DASRNX010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516337"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..996)
                     /locus_tag="VFD89_07825"
     CDS             complement(13..996)
                     /locus_tag="VFD89_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZJ58127.1"
                     /translation="MMIAMRYGKVKGLEKDVSRIVLGTDYMTTQQLDKNFSMLDTAFD
                     LGCNTLDTAHVYGGGDGERVIGRWMESRGNRDEVVILTKGAHHNADRKRVTPFDITSD
                     LHDSLARLRTDHVDIYVLHRDDPSLPVGPIVEVLNEHHAAGRIKAFGGSNWHHTRIQE
                     ANEYAQKHGLISFTASSPNYSLAEQVADPWGPGCVAIGGPKETEAREWYLQTQTPIFS
                     YSSLGRGFFSGRISRANFDETRKMLDKPSLTAYCHDQNFDRLDRVEVLAEEKGLSVAQ
                     VAMAFLMNQSLYVFPIVGVVNKKELEENIVAAKTKLTQEELDWLDLIRDDR"
     gene            complement(1019..2002)
                     /locus_tag="VFD89_07830"
     CDS             complement(1019..2002)
                     /locus_tag="VFD89_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ58128.1"
                     /translation="MEKKIRWGILGAGSIAHKFATGFGGVDDGELWAVGSRSVEKADE
                     FADQYDIPKRYGSYQELVADPDVDAIYVATPHPFHKPHTILCLNSGKAVLCEKPMAVN
                     ADEVREMVECAQKNKVFLMEAMWTRFLPVYYEVEKWLAQGLIGDIRLVTADFGFRAGV
                     DPKARLFDLNLAGGALLDVGVYTVSFASLFMKDAPCDIVSMAHLGETGVDEQSSMILG
                     YDGGRMAILYTAIRTNTIHEARIIGTEGSVYLPSFWNTTTAILKRPGQEDEVAEIPHL
                     ANGYCYEIQEVNRCIREGRLQSDRMPWDESISVMETADKLRHQWGLKYPME"
     gene            2377..5097
                     /gene="mgtA"
                     /locus_tag="VFD89_07835"
     CDS             2377..5097
                     /gene="mgtA"
                     /locus_tag="VFD89_07835"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HZJ58129.1"
                     /translation="MNKIAQDKMSNEPSGEGVQRDEVSERLIRISKSHASEIYDMLES
                     SPKGKTEAQVISARNLYGENIIIYDKDNTLAKRLAQAFINPFTLILIALAIVSIFTDI
                     ILADPAEKDITTVMIITVMVLIAGVLRFVQETRSGNAAEKLLEMIETTANIERAGVGA
                     KEIPLDEIVVGDIVHLSAGDMIPADIRILEAKDLFVSQSALTGESKTVEKISAVSDND
                     GPILAMNNLAFMGTNVVSGFSKGIVVSTGSDTIFGEIAQALNKEPTVTSFEKGVNSVS
                     WVLIRFMLVMVPIVFFINGITKGDWLGALLFAISIAVGLTPEMLPMIVTTGLAKGSVA
                     MSKKKVIIKNLNSIQNLGAIDILCTDKTGTLTEDKVRLQYHLDIDGNSDTRVLRHGFL
                     NSYYQTGLKNLLDEAIIDKTMELRDSDGNIERSITRYTKVDEVPFDFDRRRMSVVVED
                     ETGKTQMITKGAVEEMLAISGYVEYKGEVTELTEEKKKIVLDRVSDLNNNGMRVLAVS
                     QKTNPSPVGEFSVSDESDMVLIGYLAFLDPPKESAMDAIKALKFHGVTVKILTGDNEE
                     VTKSVCRQLKIEFDRVYIGSDIDGLSDHELTELAEKTTVFAKLSPTQKARIINVLRDM
                     GHSVGYMGDGINDAAALEASDVGISVDNAVDIAKESADVILLEKDLNVLENGIVEGRK
                     TYANIIKYIKLTASSNFGNVFSVLVASAFLPFLPMASLHLILLNLIYDISCIGLPWDN
                     VDEDFLERPKAWDASSISKFMLWIGPTSSVFDITTYLLMYYIISPMFTGGLLFHQLTD
                     PKMIALYIAVFQAGWFVESMWSQTLVIHMIRTPKIPFIQSRASAQLTLLTFAGIVLLT
                     IIPFTSFGASVGLAPLPAIYFLYLALTIFLYMALVTVVKGLYVRRFGELL"
BASE COUNT         1402 a         1234 c         1195 g         1388 t
ORIGIN      
        1 ctattatatt atttatctgt cgtcccttat aaggtccagc caatcaagtt cttcctgcgt
       61 cagttttgtc ttagcagcta ctatattctc ttccagctcc tttttgttta caacgcctac
      121 tatggggaaa acatatagag actggttcat caaaaaggcc atggcaacct gggccaccga
      181 taggcccttt tcctcagcta aaacctccac cctatcaagc ctgtcaaaat tttggtcgtg
      241 gcaataggcg gttagactgg gcttatccag catctttctg gtctcatcaa aatttgccct
      301 ggatattctg cccgagaaga atcccctccc aaggcttgaa taggaaaata taggggtctg
      361 ggtttgcaga taccattctc ttgcttctgt ttccttcgga ccgcctatgg caacacagcc
      421 cgggccccat ggatccgcca cctgctccgc taagctgtaa ttagggctac tggccgtaaa
      481 ggatatcaga ccatgctttt gggcatattc gttggcctct tgaatcctgg tatggtgcca
      541 gttggagccg ccaaaagcct taatccttcc ggctgcgtga tgctcattca gcacctctac
      601 aattggtcct acgggcaggg acggatcgtc tctgtgcagc acatatatat ccacatgatc
      661 tgtccttaag cgggccagag aatcatggag atccgaagtt atatcaaaag gggttaccct
      721 ctttctatct gcattatgat gggccccttt agtgaggatg acaacctcat ccctatttcc
      781 cctagattcc atccatctcc ctataaccct ttcaccatcg ccgcccccat atacgtgggc
      841 cgtgtctagg gtattgcacc ccagatcaaa ggcggtgtcc agcatggaaa agtttttatc
      901 caattgctgg gtcgtcatat aatcggtgcc caatacaatc cttgaaacgt ccttttctaa
      961 acccttcacc tttccgtatc tcatagctat catcatcgcc ccttaacata atttttattt
     1021 attccatagg atacttaagt ccccactggt gcctcagttt gtccgccgtc tccataacgg
     1081 atatggattc gtcccatggc attctatcgc tttggagtct gccctcccta atgcacctgt
     1141 tgacttcttg gatttcatag caataaccgt tcgccaggtg ggggatctct gctacctcat
     1201 cctcttgccc gggcctttta agtatagcgg tggtcgtatt ccaaaagctt ggaagataga
     1261 cagagccctc ggtcccgatt atccttgcct catggatggt attggttcga atcgccgtat
     1321 ataaaatggc catccttccc ccgtcgtaac ccagaatcat ggaggattgc tcatccaccc
     1381 cggtttcccc caggtgggcc atgctgacaa tgtcgcaagg tgcatccttc ataaacaggg
     1441 aggcaaagct gaccgtataa acgcccacgt ccagcagcgc gcccccggca agattgagat
     1501 caaacaatcg ggctttcgga tcaacgccgg cacggaagcc aaagtccgca gtcaccagcc
     1561 ttatatcgcc tatcagtccc tgtgccagcc atttttccac ctcatagtag acaggcaaaa
     1621 acctggtcca catggcttcc ataagaaaaa ccttgttctt ctgcgcgcac tctaccattt
     1681 cccttacctc atcggcattg acggccatgg gcttttcgca aagtacagcc tttcccgaat
     1741 ttaggcacaa aatggtgtga ggtttatgaa agggatgggg agtcgctacg tatatggcgt
     1801 ctacatccgg atctgcgacc aattcctggt agctcccata ccttttggga atatcgtact
     1861 ggtccgcaaa ttcatcagct ttttctacgg atctggaacc aacagcccag agttctccgt
     1921 cgtccacccc accaaatccg gtagcgaatt tatgggcaat gctgcccgca cccaatatac
     1981 cccaccttat cttcttttcc actatgcatt cctcccttta taacaccgga agcaattgta
     2041 tagataattg cagcatacag gtttcaaaac gaggtgggag atatatgtcc gtcgcctgtc
     2101 ttaactaatc gaagcccgcc agactgctta caggagtggg agacactttt ccgccccatt
     2161 aagataatga ttaaaacccg agtactgcaa tttttattcg attgctaccg gataatattg
     2221 acataccctt atacgttcac tacatattct actatatatt actctaatat aataggcccg
     2281 aatgtatttt gtcgggcaca tccctcctat tcgccccaat cggggttatt taacggggtc
     2341 tgggaaataa tgaattatga ataggaggta tttccgatga acaagatagc ccaggataaa
     2401 atgtcaaatg aaccttcagg ggaaggggtc cagcgagatg aggtcagtga aagactaatt
     2461 cgtatatcta agagccatgc cagtgaaatc tatgatatgc tggagagctc ccctaagggt
     2521 aagacagaag cccaggtgat atcggccaga aatctgtacg gtgaaaatat cataatctat
     2581 gacaaggata ataccctagc aaagaggctg gcacaggcat ttatcaatcc ctttactctg
     2641 attttaatag cccttgccat agtctctata tttacagata ttattttggc tgaccccgca
     2701 gaaaaggaca ttactacggt aatgataatc accgtcatgg tacttatagc cggggtgctc
     2761 cggtttgttc aagaaaccag aagcggtaac gcagcagaaa agttattgga gatgatcgaa
     2821 accacggcca atatcgaaag ggccggggtc ggggcaaagg aaatcccctt agatgaaata
     2881 gtcgtcgggg atattgttca tctgtccgcg ggggatatga tccccgccga tatcagaatc
     2941 ctagaagcga aggatttatt cgtaagtcaa tctgccctga cgggagagag caaaacggta
     3001 gaaaagatat cggccgtttc agataatgac gggcctatcc ttgcgatgaa caatctcgca
     3061 tttatgggga ccaatgtcgt cagcgggttt tctaagggga ttgtggtttc aacgggatcc
     3121 gatacaattt ttggagagat cgcacaggcg ctcaacaagg agccgacggt aaccagcttc
     3181 gaaaaggggg taaactcggt ttcatgggtg ttgatccggt ttatgctggt tatggtcccc
     3241 atcgtatttt ttatcaacgg gatcaccaag ggagattggc tgggtgcact tttgtttgcc
     3301 atatcaatag ccgtgggatt gaccccggag atgctgccta tgatcgtgac tacaggactg
     3361 gcaaagggat ccgtggccat gtccaagaaa aaggtgatta taaaaaacct gaactcgata
     3421 caaaacctgg gagccattga catactctgt accgataaaa cagggaccct tactgaagat
     3481 aaggtcaggc tgcaatacca tctggatatt gacggaaaca gcgataccag ggttttaagg
     3541 cacggctttt tgaacagcta ttatcaaacg ggcttaaaga atctattgga cgaggccata
     3601 atagacaaga ctatggaatt gagggacagc gatgggaaca tagaacgaag catcacccga
     3661 tatacaaagg tagacgaagt cccctttgat tttgatcgca gacgaatgag cgtagttgtc
     3721 gaggatgaaa caggaaaaac ccagatgata accaaaggtg cagtagagga gatgctcgct
     3781 atatccggtt atgttgaata caaaggggag gttaccgaac tcacggaaga gaagaaaaaa
     3841 atcgtattgg atagggtcag tgatttaaac aacaacggca tgagggtgct ggcggtttcg
     3901 caaaagacca atccctcacc ggtaggggaa ttctccgttt cagatgagag cgatatggta
     3961 ctcatcggtt accttgcctt cctggaccca cctaaagaaa gcgctatgga cgccataaag
     4021 gctttaaaat tccatggggt aacagtcaag atcttgacgg gggacaatga agaggtgacc
     4081 aaaagcgttt gtcgccagct taaaatagaa tttgataggg tctatatagg ctctgatatc
     4141 gacggactgt cggaccatga acttacagaa ttggccgaaa aaactactgt tttcgcaaag
     4201 ttatccccta cgcaaaaggc cagaataata aacgtcttaa gggatatggg ccattctgta
     4261 ggctatatgg gtgacggtat taacgatgct gccgctctgg aagcatcgga tgtgggcatt
     4321 tcagtggaca atgcagttga tatcgccaaa gaatcagccg atgtcatttt gctggaaaag
     4381 gacttaaacg tccttgaaaa cggcatagtc gaagggagaa agacctacgc caatataatc
     4441 aagtacatca aattgacggc ctcttcaaac tttggaaatg tgttttcggt attggtagca
     4501 agcgcatttt taccctttct gcccatggca tcactacact tgatcctgct gaatttgatc
     4561 tatgatatat cctgtatcgg actcccgtgg gataatgtgg atgaagactt tttggaaagg
     4621 ccaaaggctt gggatgccag ttccatctcc aagtttatgc tctggatagg gcccaccagc
     4681 tccgtattcg atatcaccac atatttgctg atgtattaca tcatatcgcc aatgtttaca
     4741 ggcggactat tgttccatca attgacagac ccaaagatga tagcattata catcgctgtc
     4801 tttcaggcgg gttggtttgt cgaatccatg tggagccaga ctctagttat acatatgata
     4861 aggacgccta aaattccctt tatccagagc cgcgcttcag cgcaactaac cctgctcaca
     4921 tttgcgggaa tagtcctgct gaccataata ccctttacca gctttggagc atcggtaggc
     4981 ttggcgccgc tccccgcgat ctatttttta tatctcgcct tgacaatatt tctctacatg
     5041 gctttggtaa ccgtcgtaaa gggattgtat gtcagaaggt ttggggaatt gctctaaaag
     5101 gggccgtatg tctggagatt tggggaatct ctctaaatgg tatcgtttgt gcctttgggt
     5161 ttgtggttaa atataaccga gaaatttaat atcacctaaa aagattggag gatatatat
//
LOCUS       DASRNX010000195         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_411645,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000195 DASRNX010000000
VERSION     DASRNX010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411645"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1760)
                     /locus_tag="VFD89_07840"
     CDS             complement(30..1760)
                     /locus_tag="VFD89_07840"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HZJ58130.1"
                     /translation="MEFNLRYQRWLNSPIIDDETKEELRSISGDEKEIEDRFYKNLEF
                     GTGGLRGVIGAGSNRMNHYTVGMATQGFADYIKKQGREAMDRGVVIAYDSRRMSPEFA
                     ERVALVLNGNGIRTFLYSELQPTPVLSFSVRELGAIAGAVITASHNPPEYNGYKVYWE
                     DGAQVTLERANAIIQNINEIQDFDEIRDMPREAALKEGLFNMVGEDVLTEYFGKVAGL
                     IQDPSLVKKMGESLGIIYTPLHGSGNKPVRRALKMAGFKNVSVVPQQELPDPEFTTVG
                     YPNPEERRVFELAIEMAEAGGSDTDIIIGTDPDCDRVGVVAKNSAGEYAMLTGNQVGA
                     LLTDYILGAMKRADNLPQNGTIIKTIVTSKMGEKIAQGYGVGVIDTLTGFKFIGEKIK
                     EFEETGSHTYLFGYEESYGYLAGDFVRDKDGVSSSMLVCEMAAYYKSKGMNLHDALVG
                     LWERYGYFYEGLESIEMEGKEGMERIQKLMGDFRCDPPKELAGIKVSVIDDYMDSKSH
                     DLKEGTSSNIELPVSDVLRYTLEDGSWFAVRPSGTEPKVKLYFSAIGSDAKEARSKME
                     NLRDAVCARV"
     gene            complement(1790..2677)
                     /gene="galU"
                     /locus_tag="VFD89_07845"
     CDS             complement(1790..2677)
                     /gene="galU"
                     /locus_tag="VFD89_07845"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HZJ58131.1"
                     /translation="MTIRKAIIPAAGLGTRFLPATKAQPKEMLPIVDKPTIQYIIEEA
                     IDSGIESIIIVTGRNKRSIEDHFDRSLELEDALRKSKNEDLLRLVDDISNMVDIHYIR
                     QKEPKGLGHAINCASAFIGDEPFAVLLGDDIVHSEVPCLKQLIGVYEKHKSTILGCQF
                     VDKNHVDKYGIVDGIPMDDDLYEVRHLVEKPRVEDAPSNLAILGRYIISPNIFKHLDE
                     TQPGAGGEIQLTDALRALAEEEAMYAFNFQGKRYDVGNKLGYLEATMEFALRNPELRD
                     NFKDYVENLISTDLFCKGE"
     gene            2826..3548
                     /locus_tag="VFD89_07850"
     CDS             2826..3548
                     /locus_tag="VFD89_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58132.1"
                     /translation="MIEIKNLSKSYNKGAVKAVEDLNLHVRPGEIFGFLGPNGAGKTT
                     TIKMMVGLLIPDSGTISLAGYDNAQNPLEAKQNIGYVPDNPDVYDRLTGMEYLNLMAD
                     VYRVSKEDRYERTNYFLNMFDLTDAVGDLIKSYSHGMKQKIVLTGALLHNPTVWILDE
                     PMVGLDPKSSHLLKEFMRKHCDLGNTVFFSTHILEVAEKLCDRIGIINRGKLIAIGSM
                     DELRHGDQAGSLENIFLELTGL"
BASE COUNT          877 a          972 c          798 g          988 t
ORIGIN      
        1 tatgataatt gcaacggttg tgtgcaattt caaaccctgg cacaaacggc atccctgaga
       61 ttttccatct tgctcctagc ctcttttgcg tcgctgccta tggcagagaa gtatagcttt
      121 acctttggct cagtccctga cgggcgaacg gcaaaccagg aaccgtcctc cagggtatag
      181 cggaggacgt cggaaacggg tagttctata ttagatgaag tcccttcctt caggtcgtga
      241 ctcttgctgt ccatgtagtc atcaataacg gacaccttga tccccgctag ctccttgggc
      301 gggtcgcatc gaaaatcacc catcaatttc tgaatccgct ccataccctc tttgccttcc
      361 atctcaatgg attccaatcc ctcgtagaaa taaccgtatc gctcccacag cccaaccagc
      421 gcatcgtgga gattcatgcc cttggacttg taataggccg ccatctcaca gaccagcatg
      481 gatgaggata ccccgtcctt atccctgaca aaatcacccg ccagataccc atagctctcc
      541 tcatatccaa agagatacgt atggcttccc gtctcttcga attctttgat cttctcgcct
      601 atgaacttaa aacccgttag ggtatcaatg acccctacgc catagccctg cgctatcttc
      661 tcgcccatct tgctggtgac aatggtcttt ataatggtac cgttttgtgg gaggttgtcg
      721 gcccgtttca tggcccccaa tatgtaatcc gtcagaaggg cgcctacctg attgcccgtc
      781 agcatggcat attccccggc tgagttttta gccaccacgc ctaccctgtc acaatccggg
      841 tcggtaccta ttattatatc ggtatcgcta cccccggctt cggccatctc tatggccagc
      901 tcaaataccc gccgttcctc ggggtttgga tagcccacgg tggtgaattc ggggtccgga
      961 agttcctgct ggggtacgac gctgacattt ttaaaccccg ccatcttaag cgccctgcgg
     1021 acgggtttgt tcccggaacc gtggaggggg gtatatatga tccccaggct ctcgcccatt
     1081 ttcttcacca gagaggggtc ttggataagg cccgctacct tgccaaaata ctcagtcagg
     1141 acgtcctccc ctaccatgtt aaagagtcct tccttaaggg ccgcttccct tggcatatcc
     1201 ctgatctcgt caaaatcctg tatctcgttg atattttgta ttatagcgtt ggcccgctcc
     1261 aaggttacct gggccccgtc ttcccaatag accttgtagc cgttatattc aggggggttg
     1321 tggctggcgg ttataaccgc accggcgatt gcgcccagct ccctcactga gaaggataat
     1381 acgggtgtag gctgaagctc gctatagaga aaggtcctaa taccgttccc atttaataca
     1441 agggctaccc tttccgcaaa ttccggcgac atcctcctgg aatcgtaagc gattaccacg
     1501 cccctgtcca tagcctccct gccctgcttt ttgatgtagt cggcaaaacc ttgggtggcc
     1561 atccctacgg tataatggtt catgcggttg gaccccgcac ctattacgcc ccgcaatcca
     1621 ccggtaccaa attcaagatt tttgtagaag cgatcttcta tctccttttc gtccccggaa
     1681 atggatctta attcttcctt cgtttcatca tcgattatgg ggctgttaag ccatcgttga
     1741 taccttaaat taaattccat atcttcacct cgccaatatt gttttcccac tactccccct
     1801 tgcaaaacag gtcggtactg atcaaattct ctacataatc tttaaaattg tcccttaatt
     1861 ccgggtttcg aagggcaaat tccatggttg cctctaagta tcccagctta ttgcccacat
     1921 cgtagcgttt gccttgaaag ttaaaagcgt acatggcctc ctcctcggcc agagcccgaa
     1981 gtgcgtcggt gagctgaatc tctccaccgg caccgggttg ggtttcatcc agatgcttaa
     2041 agatgtttgg gcttattatg tacctgccaa gaatggcaag gttggagggc gcatcctcta
     2101 cccttggttt ctctaccaga tgcctcacct catatagatc atcatccatg ggtattccgt
     2161 ctactatgcc atacttatcc acatgatttt tgtccacgaa ttggcagccc aatatggtgg
     2221 acttatgctt ttcgtatacc cctatcaatt gctttaaaca tgggacctcg gagtgcacga
     2281 tatcgtcccc caacaaaact gcgaaaggct catccccaat aaaagcactg gcacaattta
     2341 tggcatgtcc caggccctta ggctcctttt gtcttatata atggatgtct accatattgg
     2401 atatatcatc taccaaccgc aatagatcct cgttcttgct ctttctcagg gcatcctcca
     2461 actccaagga cctatcaaaa tggtcttcga tggacctttt gtttcgcccc gtaactatta
     2521 ttatgctctc aattccggaa tcaatagcct cttcaattat gtactgaatg gttggcttgt
     2581 ccactatggg cagcatctcc ttgggctgcg ccttggttgc cggtaaaaac cgggtcccca
     2641 ggccggcagc cgggattata gcctttcgta ttgtcaactc caacacccca atctaaaaat
     2701 ttacatcttc tgtatactac cgattatagc atattaaaga cccaatcttc aattaaatcc
     2761 gaatatccac tggtgaaatt tgtataaaac tatggtagaa tcaataaagg taaaggagtg
     2821 atgacgtgat agagattaaa aatctttcca agagctataa caagggcgca gttaaggcag
     2881 tagaggactt aaacctccat gtgaggcccg gggagatatt cgggttttta ggccccaacg
     2941 gtgccggcaa gaccacgacc atcaagatga tggtgggact cttgattccg gatagcggaa
     3001 ccatcagtct cgcaggatat gacaacgccc aaaatccctt ggaagccaag cagaatatcg
     3061 ggtatgtacc ggataacccc gatgtgtacg accggttaac cggcatggag tatctgaatt
     3121 tgatggcgga tgtatacagg gtctccaaag aggatagata tgagcgtaca aattattttc
     3181 tcaacatgtt tgacctaacg gatgcagtgg gcgacctcat caagagttat tcccacggta
     3241 tgaagcagaa gatagtgctg acaggggccc tccttcataa cccaaccgtc tggatattgg
     3301 atgagcctat ggtggggctg gacccaaagt cttcccacct tctgaaggag tttatgagaa
     3361 agcattgtga cctgggtaat acggtcttct tttccaccca catattggag gtagccgaaa
     3421 agctctgcga ccgaatcggc attataaata gaggaaagct gatagccata gggagtatgg
     3481 acgaactcag gcacggcgat caggcgggat cccttgagaa tatcttcttg gagctgacgg
     3541 ggctatgagt agatttattg cacttttaaa agcgagtctc aacgtccatt tcggtatttc
     3601 ttctctgaaa taccgcttta ctaaggaaaa aaagc
//
LOCUS       DASRNX010000196         5014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_411825,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000196 DASRNX010000000
VERSION     DASRNX010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411825"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VFD89_07855"
     CDS             complement(<1..595)
                     /locus_tag="VFD89_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HZJ58133.1"
                     /translation="MSINSMYSLNRMRFGGLASGLDTDTIVRDLMRIEQMKVDKLKQE
                     KQIMEWRKEDYRSTTNLLRGFYDEYFDILYSKTNMTSQSAYNILKVQSSDEKYVTATA
                     TTGAYVSDRTISKIEMAKAAQLEGTSVSAPLESGELTGDINLVGLHRITVTLDGATKV
                     IEFDVKENQIQGQDGLLEAFQGGLDKAFGMDRINVAFE"
     gene            complement(654..1052)
                     /locus_tag="VFD89_07860"
     CDS             complement(654..1052)
                     /locus_tag="VFD89_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HZJ58134.1"
                     /translation="MRVIGPISAGRTVDINRGNAPETQAVDEKVITDKGASQRDLPQN
                     FRTMDPKKNEPITIGELELIEAIERANNTLRGINTNFQFSIHEDTKAIMVKVINSDTK
                     EIIREIPPEKILDMVAKMWEMAGIFVDRHI"
     gene            complement(1175..1564)
                     /locus_tag="VFD89_07865"
     CDS             complement(1175..1564)
                     /locus_tag="VFD89_07865"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZJ58135.1"
                     /translation="MMLTRKQRAQLDKVATLIGEDVVLKDGILYAQNTLRIDGQYRGE
                     INSKGAVVIGGNGIVDGSIRGENIIVSGKVNGDIKSSNQVHIRDTGSVTGDVSCLTII
                     IEEGGIFMGKCNITKAIAGEVMSEADE"
     gene            complement(1561..2616)
                     /locus_tag="VFD89_07870"
     CDS             complement(1561..2616)
                     /locus_tag="VFD89_07870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZJ58136.1"
                     /translation="MKTRIKKKRSRYLNIVIVPDAEFRPKTLRIPKWAISSLTGIAVV
                     GIMAFSGMILYTHWLGGQLTIKEDKLQAYKGRVANNESVIESLEGEIDYYSGELKSIM
                     SVAFSLEEKTSEIADNNNQILGEIGHLLGLESMEKELESIVEKAAHDIGTLSSRSGSR
                     PTPEPQDKTTDLSLEQNYVRTVNEFVAADKRVSDAEGFNEHLSKKVSEMQDYLLAYPS
                     ILPVEGRITCPMGERDNPFGGKKKEYHSGLDMAAPTGTDVKATGKGRVIFSGAKGGYG
                     NLVIIDHGFDLKTYYAHNSRLLVEVGDRVERGDIIAKSGSTGRSTGPHVHYEVRIKDI
                     PKDPLDFALNFNEGDDE"
     gene            complement(2718..3380)
                     /locus_tag="VFD89_07875"
     CDS             complement(2718..3380)
                     /locus_tag="VFD89_07875"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZJ58137.1"
                     /translation="MSIITVDFDGTLYQHNSIIATLKGGKELFAFRQWVYILGDIIKG
                     LVKDVIGGGSSFRILFLESFFRQMKGKSMEELHGFFESLVTAGHQGINFDLVSRLAKH
                     FEDGDRIIVLSGGLQPFLEVFIQKLGLQANAIGTLLFYDDEGICTGEIGKINSGAEKV
                     NRLNLWINENDAGGETVWAYADSESDIPLLEFADKAIVVNPSKALKDIALSKGWEFCE
                     PV"
     gene            complement(3447..4703)
                     /locus_tag="VFD89_07880"
     CDS             complement(3447..4703)
                     /locus_tag="VFD89_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZJ58138.1"
                     /translation="MTSEISLPTGRGGLKIDTKDTTYYSYKEIGQPDEENIRIFICDS
                     IAKRILKGSKNPVYPKTFGLLLGSKIQKGDGFIIHINEAMPLGRLKVHGNPKALSQKS
                     WETVQNKVYNLYSGMSILGWYGLRNGWEAMLTHQDRAIHNEHFIKSWQVIYLLDGSNG
                     SSNFFCWDGDKLKPSGGYYEYRDEDISESGKNKLGNQKKLIIGLIGAIIIILTVYLSG
                     QYGEGYFSPKDNQNIAGDVQPENTGDNLGDNYGNGNDRGNETAPGQDTSEKQGSAQSD
                     HVQDGVIRAGEDGPNHTVEDYQAALVDLESKLKGKQQKIENLEQVVESLEKETKGLDE
                     EIKDIIQEIEEKEAADKLDEGLSDGADIYVIQSGDNLYKISQRFYGTDRYSQALAKVN
                     RIKDQKALVVGDYLVIPSIEEVEKWD"
BASE COUNT         1211 a         1293 c          921 g         1589 t
ORIGIN      
        1 cctcaaatgc tacgtttatc ctatccatcc caaaagcttt gtccagtcca ccctgaaaag
       61 cctctagcaa gccatcttgg ccttggatct gattttcttt aacatcaaat tctataacct
      121 tggtagcgcc gtctaaagtg acagttattc tgtgaagacc caccaaattt atatccccgg
      181 tcaactcccc gctctctaaa ggggccgaaa ccgacgtacc ttcaagctgg gcagccttcg
      241 ccatctctat cttcgaaatg gttctgtcgg atacatatgc gcctgtagta gctgtagcgg
      301 ttacgtattt ttcatcactg gattgtacct ttagtatgtt ataggcactt tgtgaggtca
      361 tattggtctt ggagtagagg atatcaaaat actcgtcata aaaccccctg agaaggttgg
      421 tggtgcttcg ataatcttct ttgcgccact ccattatctg cttttcctgc ttaagcttgt
      481 ccaccttcat ctgctcaatc ctcataagat ccctgacgat ggtatcggtg tccaggccac
      541 ttgccaagcc tccaaacctc atccggttta atgaatacat ggaatttatg ctcatatcct
      601 cacctctcgc cttcctaaat ccttaagttc tgtaatcttt aagccctgcc agcttaaata
      661 tgcctgtcta caaatatccc tgccatctcc cacatcttag ccaccatatc caaaatcttc
      721 tccggaggaa tctctcggat tatctctttg gtatcgctgt ttatcacctt taccatgatg
      781 gccttggtat cctcatggat ggaaaactgg aagttggtat ttatgcccct tagagtgttg
      841 ttggctcttt ctatagcttc gatcagctct aattcgccta tggtaatggg ctcgttcttt
      901 tttggatcca tggtcctaaa attctggggc agatcccttt gggaagcacc cttgtctgtg
      961 atcactttct cgtctactgc ctgggtttca ggagcattgc cccggtttat atccactgtc
     1021 ctgccggcgg atatgggacc tattactctc atggcgttcc ccctccaatc tctttctaca
     1081 aactatatcg gaagatcagg gataaaactt taggggtttg ccataaaatt aattccgctt
     1141 cgacttttag ccgctgattt ctaatcgctc ctttttattc gtcagcttcc gacatgactt
     1201 cgccggcgat agcctttgtt atattacact ttcccataaa tataccaccc tcttcgatta
     1261 tgatggtaag gcaagaaaca tcacctgtta cagagccggt atcccttata tgaacctggt
     1321 tggaggactt gatgtccccg ttaaccttcc cggaaacgat tatgttctcg ccccggatgc
     1381 tgccgtccac tataccatta ccccctatga caacggcacc tttactgttt atctcgcccc
     1441 tgtactgacc atcgatcctc agggtatttt gagcatacaa aatcccgtcc tttaaaacaa
     1501 catcctcccc gattaaagtc gcaaccttat cgagttgcgc cctctgcttt ctggttagca
     1561 tcattcatcg tccccctcat tgaaatttaa tgcaaagtcc aacggatcct ttggaatgtc
     1621 ctttatcctt acctcataat gaacatgggg gccggtgctc cgacctgtgc tgccggattt
     1681 tgcgataata tcgccccttt ctactctgtc ccccacctcc accagcaacc tggaattatg
     1741 ggcgtaatag gtctttagat caaagccgtg gtcaattata acgaggttcc catagccacc
     1801 cttggcgccg gaaaagatca cccgcccctt tcccgtagcc tttacatcgg tccctgtggg
     1861 agctgccata tcaaggcctg aatgatactc ctttttcttg ccgccaaaag gattatcccg
     1921 ctcccccata gggcaggtta tccttccctc caccggaagg atggagggat aggccaaaag
     1981 gtaatcttgc atctcagata cctttttcga taaatgctca ttaaagccct cagcgtctga
     2041 aaccctttta tccgccgcaa caaattcatt gaccgtccga acataatttt gttccaggga
     2101 tagatccgtg gttttatctt ggggttcagg cgtcggtcgg gaaccggacc tggaagatag
     2161 agttccaata tcatgggctg ccttctccac tatggattca agttcttttt ccatggattc
     2221 taggcccaat agatgcccga tttccccaag gatctgatta ttattatctg cgatctccga
     2281 ggttttttct tctagggaaa atgctacgga cattatggat tttagttcac ccgaatagta
     2341 atctatttcg ccttccaggc tctctataac gctttcatta ttcgcaactc ggcctttgta
     2401 ggcctgcaac ttatcctcct tgatagtcag ctgaccgccc agccaatggg tatagaggat
     2461 catccccgaa aatgccatga tgcccactac tgctatgccg gtaagggacg atatcgccca
     2521 ttttggtatt ctaagggttt taggcctaaa ttccgcatcg ggaacaatga ctatattcag
     2581 gtacctgctc ctcttctttt taatccttgt cttcatattt actccatcct tatatgtata
     2641 tgtttgacgg tatgtcaaat ggctttttcc agattaatta accatgctga atataataca
     2701 accaatacga tatattatca tactggttcg cagaattccc atccctttga taatgcgatg
     2761 tcttttaatg ctttggacgg atttacaaca atagccttat ctgcaaattc gagcaatgga
     2821 atatccgatt cgctgtctgc atatgcccag acagtttccc caccggcatc gttttcattt
     2881 atccacagat ttagtctatt tactttctca gccccgctat ttattttgcc gatctctccc
     2941 gtacagatcc cttcatcatc ataaaacaat agtgtcccaa ttgcgttcgc ctgtaagccc
     3001 agcttttgaa tgaacacctc taaaaagggt tgcaggcccc ccgataaaac gatgatacga
     3061 tcaccgtctt cgaaatgctt ggctaatctt gataccagat cgaaattgat gccttgatga
     3121 ccggcagtaa ctaaggattc aaaaaatcca tgcaattctt ccatgctctt tcccttcatt
     3181 tgcctgaaaa aagattccag aaaaagtatg cgaaaacttg accccccgcc tattacgtct
     3241 tttacaagcc cttttattat atccccgaga atatataccc attgtctaaa ggcaaataac
     3301 tccttgccgc ccttaagtgt agcgatgatt gaattgtgct gatatagggt cccatcaaag
     3361 tctaccgtta taattgacat atcttagctc cttactattt ttaaagccca atattaggtg
     3421 aatcttagag tatcaggaaa aaacatttaa tcccactttt ctacctcttc aatggatggg
     3481 attaccaaat aatcccccac taccagagcc ttttggtcct ttattctatt tacctttgcc
     3541 aatgcctggc tgtacctgtc ggtcccatag aacctttggc tgatcttgta taggttgtca
     3601 ccgctttgga ttacgtagat atcagcccca tctgaaagcc cttcgtctaa tttatccgca
     3661 gcctctttct cctcaatctc ctgtattatg tcctttatct cttcatctag gcccttcgtt
     3721 tctttttcca ggctttcaac cacctgttcc aaattctcta ttttctgttg tttacctttc
     3781 agcttgctct caaggtcaac taatgcagcc tgataatctt ctaccgtatg gttaggccca
     3841 tcttctccag cccgtattac gccgtcttgg acatggtcgc tttgagctga cccctgcttt
     3901 tctgatgtgt cttgacccgg agcagtttca ttacctcggt catttccatt cccgtaatta
     3961 tctcccaggt tatcgccggt attttctggt tgcacgtccc cggctatatt ttgattatct
     4021 ttaggagaaa aataaccctc gccatactgg ccggatagat agaccgtgag aataattatg
     4081 atggcgccaa taagaccgat tatcagtttt ttttggttac ccaatttgtt tttgccggat
     4141 tctgatatat cttcgtccct gtattcataa tacccgccgc tgggcttcag cttgtcccca
     4201 tcccagcaaa aaaagttact ggatccgttc gatccatcca acagatagat aacctgccag
     4261 gactttataa aatgctcatt atgtatggcc cggtcctggt gggtcagcat tgcctcccat
     4321 ccgtttcgaa gaccatacca tccaaggatg ctcatccccg aatagaggtt atacaccttg
     4381 ttttgaacgg tttcccaact cttttgagat agggctttag gattaccatg gacttttagt
     4441 ctgcccaagg gcatggcctc atttatatgt attataaacc cgtcaccctt ttggatcttg
     4501 ctaccgagga gcaggccaaa agtctttgga tagaccgggt ttttgctccc ctttaagatc
     4561 cttttagcaa tactgtcgca gataaatatc ctaatattct cctcatccgg ctgaccaatc
     4621 tctttataac tataataagt agtgtccttg gtatctattt tgagtcctcc tctacccgtt
     4681 ggtagtgaaa tttcagacgt catccctgtt gcgatgactt tttggaatac cacctttatc
     4741 ttacactatt ttaatgccaa aatcgatccc aatttaacag gaaggagagc ccccatgcct
     4801 atgataatgg gatcgccttt gaagtcttta gtaagagatg tgattgaaag tgatgagatc
     4861 ccagttgata taaaagtgcg aacagtgtat aatattgcca attaatgcgg ctgtattgta
     4921 cattagccct ggtggagata tagaaactac atccaactgg agatgagtat agatttcata
     4981 tgtttttatt tgaataaggc aaacagggct atca
//
LOCUS       DASRNX010000197         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_412276,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000197 DASRNX010000000
VERSION     DASRNX010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412276"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFD89_07885"
     CDS             <1..369
                     /locus_tag="VFD89_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58139.1"
                     /translation="GYFMSDLNTAPNNTGSEEIPVFDQEDIEKNKAMAGLAYFIFFLP
                     LVACPESQFGKFHANQGLLLLILGLGGSLVLSLIPIIGWMILPIYPIFIGILGIMGLI
                     NGFGGKAKELPLIGKYRIIK"
     gene            398..910
                     /locus_tag="VFD89_07890"
     CDS             398..910
                     /locus_tag="VFD89_07890"
                     /inference="COORDINATES: protein motif:HMM:NF042527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6591 domain-containing protein"
                     /protein_id="HZJ58140.1"
                     /translation="MLRKLIILSICIAVLLSFVGCGYKRKVEEKLANKITEGILDKVA
                     GNNTNVNIEDDKITIEGEDGSKLAIGSSEWPEGKAADLIPEFKRGKIASTMDMGASCT
                     IIVDDVERKDFDEYLQVIKDKGYTKDSMDMSTEELISYFASYEKDKSTISLGYTMETK
                     EMMISITIQE"
     gene            1095..2810
                     /locus_tag="VFD89_07895"
     CDS             1095..2810
                     /locus_tag="VFD89_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZJ58141.1"
                     /translation="MKVIKRAQGWLLMLMMVLSMLLAPAAAYAGNDMDTRSLALINKP
                     GVVLLQSTWYADVVIDQLSIDEGIFEVIDVAIEQMGEEGQLTGDMDQFTSIYIQIFTQ
                     LLPDYTYPSGIPTATSAQSSFIGTGFIVTPDGYLLTNAHVVETDEQTMLMGFAYDAAA
                     SYVAGEIQEFAQGIYDAMGVVPAQEDLDLLAETYYSLLIQEMQVNNLSAEYLCFMGNT
                     IGGTDPSVKAVTLDLRKKGASIPDKDIAILKMDKTNLPTVALGDDSMIKTGDTVYAMG
                     YPAVATVNGTLNIDQAIQEPTLTNGIISAKKQMAGGWSILQTSADIHGGNSGGPLFNE
                     SGEVVGINTFGMLEESGASASGMNFAIPISIAKQFLSEINVTSSESQFTTDYRKAVML
                     YGQEDYRGAADLLRQINEMNPGYPVVVELLSECSSLAGAQPVSTQEAPVTADADDVID
                     DEESPKQTDKEADLKPIPWLPIGLGGGGLVLVVIIIISVAAVKKRKRIQPPKPEVTAH
                     PSTADEPDGSICPICAVFNVKGIKFCKECGGKLDTPAPTIKCQACGMENIPGTKFCGE
                     CGGKL"
BASE COUNT          911 a          596 c          749 g          757 t
ORIGIN      
        1 ggttatttca tgtctgatct aaatactgct ccaaacaaca ctggaagtga agagatccct
       61 gtttttgatc aagaggatat tgagaagaac aaagccatgg ctgggctggc ctattttatc
      121 tttttcttac cgctggtagc ttgtcctgag tcccagtttg gaaaattcca tgcaaatcaa
      181 ggattattac ttctcatcct tggattaggt ggtagtcttg ttctaagcct cattccaatt
      241 atcgggtgga tgatattgcc aatataccct atatttattg gaatactagg gatcatggga
      301 ttaataaatg ggtttggtgg taaagccaag gaacttcctc taataggaaa atatagaata
      361 atcaaataga acccaataag aaagaaggta tacttttatg ctaagaaaat taattatact
      421 atccatatgc atagcagtac ttctatcatt tgtaggctgt ggctataagc gaaaagttga
      481 agaaaagcta gccaataaaa taacagaagg tatcttagac aaggtcgcag gcaataatac
      541 taatgtaaat atagaggatg acaagattac tattgaaggt gaagatggat caaaacttgc
      601 tataggcagt agcgaatggc cggaaggtaa ggctgcagat ctaattcccg agtttaaaag
      661 aggaaagata gcatcgacta tggatatggg tgcctcttgt accatcatag ttgatgatgt
      721 tgaacggaag gatttcgatg aatatcttca agtaataaag gataagggct ataccaagga
      781 tagcatggat atgtctacag aagaactaat aagttatttt gcctcatatg aaaaggataa
      841 atccaccata agtctaggct acacaatgga aacaaaagaa atgatgatat ctattacaat
      901 acaggagtaa agcttttagt acagagactg tctaggcact agataatctc tgtacaatct
      961 ttgaatcaag ttccacctta acctcgcact attaacatta gaagatctca attagaaata
     1021 cgaaatttga aataaaaaat aagtttggac cattcaaaac tagatctaat catattatgc
     1081 ggggaggaat ccagatgaaa gtaataaaac gagcacaggg ctggttgttg atgcttatga
     1141 tggtgctgag catgttgcta gcaccggccg cagcttatgc tgggaatgac atggacacac
     1201 ggagccttgc acttataaat aaaccggggg ttgttttatt gcaatccaca tggtacgccg
     1261 atgtagtaat tgaccaactg tccattgatg agggaatctt cgaggtaatc gatgtagcta
     1321 tagaacagat gggcgaagag gggcagttga caggtgacat ggatcagttt acctcgattt
     1381 atatacagat tttcacacag cttttgcccg attacaccta cccaagtggc atcccaaccg
     1441 ccaccagtgc tcaaagttcc tttataggca caggtttcat cgtgacgccg gatggctatc
     1501 tgttgactaa tgcccacgtg gtggaaactg acgaacagac catgttgatg ggatttgcct
     1561 atgacgcagc agcaagctat gtggctgggg aaatccaaga gttcgcacag ggtatctacg
     1621 atgccatggg tgtcgttcca gcacaagagg atctggacct actagcagaa acctactatt
     1681 ccttgctaat tcaggagatg caggtcaaca acctttccgc cgaatatctg tgtttcatgg
     1741 gcaacaccat agggggaacg gatccctcag tcaaggctgt taccctggat ctgcggaaaa
     1801 agggcgcatc catcccagac aaggatatcg ccatcctcaa gatggataag accaatctgc
     1861 ctacggtggc gctgggcgac gatagcatga ttaaaactgg ggataccgtc tatgccatgg
     1921 gttatccggc agtcgctaca gtgaacggaa ctctgaacat cgatcaggct atccaggaac
     1981 ccacgctgac caatggcatc ataagcgcta agaaacaaat ggcggggggt tggagcattt
     2041 tgcagaccag tgcagatatc catggaggta atagcggggg acctctattc aacgagagtg
     2101 gcgaggtcgt gggtattaac accttcggta tgctagaaga aagcggtgca tctgcttcgg
     2161 gcatgaattt tgctattccc atcagcatcg ccaaacaatt ccttagcgaa ataaatgtca
     2221 catcctcgga aagccagttt accaccgact ataggaaggc agtcatgttg tatgggcagg
     2281 aggattacag gggcgctgcg gatttgcttc gccagattaa tgagatgaac cccggatatc
     2341 ccgtggtggt ggaactgctt agtgaatgta gttcccttgc gggtgcacag ccagtatcta
     2401 cacaggaagc accggttact gctgatgctg atgatgttat tgatgatgaa gaatccccaa
     2461 agcaaacgga taaagaggcc gacctgaaac caattccatg gctccccatt ggtttaggtg
     2521 ggggtggact cgtcctagtg gtgatcataa tcataagcgt agccgctgtg aaaaaacgta
     2581 agaggataca gccccctaaa ccggaggtga ctgcccatcc aagcactgct gatgaaccgg
     2641 atggctccat ttgcccgatt tgtgccgtat tcaatgtgaa ggggataaaa ttctgcaagg
     2701 agtgtggtgg gaaactagat acccccgctc ctacaataaa gtgccaggcc tgcggcatgg
     2761 agaatatacc cgggacgaag ttctgtggag agtgcggcgg caagctgtag aatctttaat
     2821 cgagttccat cataaaatat tggaggttgg agaaacatgg ccttttataa gcaaagctgt
     2881 attcgatgtg gctcccttat agaccgggat tgagctagcc aagggagtca aattttgcgg
     2941 ggaatgcgga gcaagagcat gattaggggg gactgagaat gggggaatcc ggaaccgaat
     3001 tatactatct gtc
//
LOCUS       DASRNX010000198        11006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_724319,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000198 DASRNX010000000
VERSION     DASRNX010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11006
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724319"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /locus_tag="VFD89_07900"
     CDS             <1..1528
                     /locus_tag="VFD89_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZJ58142.1"
                     /translation="LYDKKTDKVIAGASTMGLTEVGTTKMTGTRVSFMPDDRVFEDIN
                     FNAETIAKRLRELTFLNRGLEIVLKDLRQGADSDVIPKRVFLYNGGLMDFVEYINENK
                     SPIHPEIVFLEGEKDGIYAQIAIQYTDSYTESIFSYVNNIPTSEGGTHETGFKTAITK
                     VLNDYARKFNYLKERDNNLNGEDYREGMTALISVKMRNIQFEGQTKTKLGNSEARTAL
                     EAITTEQLIHFIENTTHEHVIRLIIEKAIKAAKVRDAARKAREITRKKNSLEGAPLVG
                     KLASCTGRDVSINELFIVEGDSAGGSAKQGRDRRFQAILPLRGKPLNVEKKRLDQVLA
                     NEEFRTIISALGTGIDEDFNIKLLKYHRIIILSDADQDGAHIRAILLTFFFRYMKELI
                     TEGHVYIGLSPLYKIAKGKTVEYAYNEVELKEKIKKVGRGYTVQRYKGLGEMNPDQLW
                     ETTMDPQNRSIIQVNIDDAAQADKLVTVLMGDKVDPRRQYISTYANFNKLDVFQEMGV
                     "
     gene            1532..4000
                     /gene="gyrA"
                     /locus_tag="VFD89_07905"
     CDS             1532..4000
                     /gene="gyrA"
                     /locus_tag="VFD89_07905"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZJ58143.1"
                     /translation="MAKNRKTIDEFVQNKDLIIHKPLEEVMHESIMPYAEYIILERAL
                     PRVEDGLKPVQRRILYTMHELGVTPEKPHRKSARVVGDALGKYHPHGDTSVYDAMVRM
                     AQNFVMRAPLVDGHGNFGSMDGDSAAAMRYTEVRMTPLATELLKDIGKDTVDYSLNFD
                     DTLKEPDVLPGRFPNLLVNGASGIAIGLATNIPPHNLAEVIDGVILQMENPDIALDQL
                     LNVIKGPDFPTGGVIIGQEEIQKAYETGRGKIIVRAKVDVEDIAGGKKLLVIKELPYQ
                     VNKATLLERILRLSEERKGVLTGIADIRDESDRNGVRAVIEVKRDGDAEKILNYLYKY
                     SDLQVTFGANMVAIAHGRPQQLGLKDILGHYIQHQKEVVTRRTRYDLDRAKAREHILE
                     GLIVAIHNIDRVIAIIRESKNPGIARTNLMTEFVLTEIQAQAILDMRLQRLTNLEVVS
                     LEREYREVKKTIKRLEVILASEKRLIGLIKKELNEVKDKYSSGRRTGIIKDDSEAEIR
                     TEDLIHVEECVVTLTRNQDIKRVPLKSFNRSSKDVEVVDTREMDFIEFLIDSETDHRI
                     LLLTDRGNSYSLDALEIPEEKWRDKGSQLMSLIKGFDRNERIIAMFSIKEFEEDLFIQ
                     FYTAMGQVKKTSLAEYNTRRSRIQACGLNQGDLCIGAELVAQDKETIIVTAEGMSIRF
                     NGAEVNATGRTAKGVRGIQLKGDDRVVFGGQIEGEGNIIVVTDRGIGKRSSVADYQVQ
                     GRGGIGFKTLTFYKNRANGRYILGAFYAKAPYEVILQQTDGTIARLNTNDLPLAARDG
                     RGSTIVTVAEDNIVEYVYRNYNDA"
     gene            4302..5078
                     /locus_tag="VFD89_07910"
     CDS             4302..5078
                     /locus_tag="VFD89_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZJ58144.1"
                     /translation="MRVIYISKKKTARVLLIFCLVLVSVAYSINFRDNAVAIFLNTKR
                     ELPIYCVERDDKRISISFDAAWGTEYTDELLEILDERNIKCTFFLVGFWIDAHPEKVR
                     LIAQKGHEIGNHSTNHPHMSNLGKEQIIKEIETTQEKIEELAGDRAVKLIRPPYGDYN
                     NLLISTCRELGFYPIQWDVDSLDWKELGVDHMYRQVTGKVKPGSIVLFHNNAKYTSTA
                     LPSILDKFLDEGYEFVPISQLIYKDSYDIDHTGMQKKKGS"
     gene            5393..6043
                     /locus_tag="VFD89_07915"
     CDS             5393..6043
                     /locus_tag="VFD89_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZJ58145.1"
                     /translation="MTEHIAENNKVVVVGKVHSPMEFSHEIFGENFYQFDLSVPRLSD
                     YVDLLPITISERLLGGIELAEGMEIIIEGQLRSYNKFIGGYNRLILTVFARELLLKDE
                     GIKNPNQIYLNGFICKEPVYRVTPFNREITDILLAVNRAYGKSDYIPTIAWGRNARFS
                     QHLNVGDNIRIWGRIQSRTYQKKLPKGEVLEKVAYEVSISKMEVARDEMESQTLET"
     gene            6302..6598
                     /locus_tag="VFD89_07920"
     CDS             6302..6598
                     /locus_tag="VFD89_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HZJ58146.1"
                     /translation="MKYKSKLRNQQVDELFDAVLELRDLEECYRFFEDICTINEVKSL
                     AQRLGVAKMLRAGRTYQDIADETGASTATISRVNRSLTYGADGYNIILDRLLDK"
     gene            6712..8970
                     /gene="pcrA"
                     /locus_tag="VFD89_07925"
     CDS             6712..8970
                     /gene="pcrA"
                     /locus_tag="VFD89_07925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HZJ58147.1"
                     /translation="MELTRLLNPQQKKAVLHTKGPLLVLAGAGSGKTRVLTYRVAYLV
                     EKLGVRAGNILAITFTNKAAKEMNERIHSLVGSKSRDIWISTFHSTCVRILRKEIERI
                     GFTRNFVIYDADDQLTLIRDCIKELDLNEKYFNAKDIRFRIGQLKDQIKSPADYRRET
                     GGRFREEKIADLYELYQNRLAKNNALDFDDLINRTLELFRLRPEVLDYYASRFQYILV
                     DEYQDTNHAQYMLIKLLSTKHQNLCVVGDDDQSIYGWRGADVRNILEFERDFPNAEVI
                     KLEENYRSSQVILDAANEVIRNNSGRKEKRLWTQKGQGEKIVVYKVPDEHREADMICR
                     HIHGHIVEDNKAPGDFAVLYRTNAQSRVIEDALMRYGIPYRMYGGLRFYDRKEIKDII
                     AYLRVVANPADDISIKRIINTPRRGIGNVTLGNLEEISIREDESIFSIILDLDQYREF
                     TPRMGKMIKEFGEAISNLIAMKEVMPLTEFIEALLEHTGYEQAIKDEGTLESETRLEN
                     IREFISAARDFEGDYEDAGLVEFLENVALVSDLDNLGEGQSAVSMMTMHSAKGLEFPM
                     VFIAGMEEYLFPHSRSMDSQQEMEEERRLCYVGITRAKERLYLSHAVRRNIFGNYNRN
                     SPSRFLEEIPQTLMEYSDQSLEDIWGTRQNRNITGNRYAGNGRDKVGVGALGSQPGPF
                     QTSGTPMGVNKGGTHGIRNFSLGQKIVHKKFGEGTIVAMSGSGDDQELKIAFEQGGIK
                     SFMATYAPLKKL"
     gene            8989..10992
                     /gene="ligA"
                     /locus_tag="VFD89_07930"
     CDS             8989..10992
                     /gene="ligA"
                     /locus_tag="VFD89_07930"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZJ58148.1"
                     /translation="MDKSDILKKIESLRDEIRHHNYQYYILDRPIISDTEYDSLIHRL
                     IDLEEENPEFYSPDSPTQRVGGGSLEEFGTAVHSSPMLSLANAFSPDDLLDFDRRVRG
                     MLEKEVEYIAEFKIDGLSVVLEYENGVFTRGATRGDGLVGEDVTENLRTVRSIPLRLN
                     KPYSLETRGEIFISKEHFKQLNQRRELHGQQTFANPRNAAAGSLRQLDPRITASRHLD
                     IFLFNLERVEGVDIHSHLQTMDVMKDAGLKISPFLYTTTSMEEIIELCIEWTDKRHDL
                     PFDIDGLVIKVNSIGQRQLLGATSKTPRWAIAYKFPAQQEETRLLEIEVQVGRTGVLT
                     PIAILDPVQIAGSTVARASLHNEDYIMEKDIRIGDYVIIQKAGDIIPEVVRVQKDKRN
                     GDERNFEMPDRCPVCGAHTVRIEGEAAIRCTGNACPAQQRRLVMHYVSRDAMDITGLG
                     PALVDQLIENDLIGDAGDLYYLDYNQLIHLERMGDKSADNLLKAIEISKKNELRRLIF
                     GLGIRLVGVRAAQLIAQKFGHLDDLLRAGEDDLLAIDEIGPKIAQSVTAFFGEEQNLR
                     LIDKLRRAGVNFSQSQRASDQENGAPTHGWRDKTFVLTGTLKEYTRNDAKSLIEERGG
                     RVTGNVSKKTSYVLAGENPGSKLNRARDLGIVIIDEDQFNSML"
BASE COUNT         3426 a         2024 c         2851 g         2705 t
ORIGIN      
        1 cctgtacgat aaaaaaactg acaaggtaat tgccggggcc tcaaccatgg gactcacgga
       61 ggttgggaca acaaaaatga caggaacccg ggtgtccttt atgcctgatg atagggtttt
      121 tgaagatatc aattttaatg ccgagaccat agccaaacgt cttcgagaat tgaccttttt
      181 aaataggggc ctagaaatag ttctgaagga tttgaggcaa ggggcggata gcgacgtaat
      241 ccctaagagg gtttttttat ataatggcgg attgatggat tttgtggaat atataaacga
      301 gaataaatcc cctatacacc cagagatagt atttctggaa ggggaaaagg acggaatata
      361 tgcacagatc gccatccagt ataccgactc ctacacggag agcatcttct cttatgtaaa
      421 caatattcct accagtgagg ggggcaccca cgaaaccggg tttaagacgg ccataacgaa
      481 ggtgttaaac gactatgccc gaaaatttaa ctacctaaag gaaagggata ataaccttaa
      541 cggtgaagat tatcgcgagg gtatgaccgc actcatttcc gttaaaatgc gaaatatcca
      601 atttgaggga cagaccaaaa ccaagttggg aaattccgaa gccaggactg ctctagaagc
      661 tatcaccacg gagcagttga tacattttat tgagaacact acccacgagc atgttatcag
      721 gcttattata gaaaaagcta ttaaggcggc caaggtcagg gatgcggctc gaaaggcacg
      781 ggagattaca cgaaagaaga acagcttaga gggggcaccc ttggtaggca aattagccag
      841 ctgtacaggc agggatgtat ccattaatga actctttatt gtagaggggg attcggcggg
      901 gggttccgct aaacaaggca gggataggcg atttcaggca attttgcccc tacgaggcaa
      961 acccttaaac gtagaaaaga agcgtttgga tcaggtctta gccaacgaag agtttcgaac
     1021 cataatttct gccctgggta ccggcatcga tgaggacttc aatataaagc ttcttaaata
     1081 ccacaggatt ataatccttt cggatgcgga tcaggacggg gcgcatattc gtgcaatact
     1141 tcttaccttc tttttcagat atatgaagga attgattacc gaaggacatg tgtatatcgg
     1201 gctctccccc ctctataaga tagctaaggg gaaaaccgtg gaatatgcct acaatgaggt
     1261 ggaattaaag gagaaaatta agaaggtggg taggggatat acggtccaaa gatataaggg
     1321 attaggagaa atgaatccgg atcagctatg ggaaaccacc atggaccccc agaaccgttc
     1381 cataatccag gtaaatatag acgatgcagc tcaggcggac aagctggtaa ccgtccttat
     1441 gggcgataag gtggatccca ggcggcaata catcagcacc tatgctaatt ttaataaatt
     1501 ggatgttttt caagagatgg gggtataagt cgtggccaag aacagaaaaa ctattgacga
     1561 atttgtccaa aataaggatc tcataataca caaaccactg gaagaggtta tgcatgaatc
     1621 cattatgcct tatgcagaat atataattct ggaaagggcg cttccaaggg tggaggacgg
     1681 cctcaagccc gtccaaagaa ggattctgta taccatgcat gaattggggg taaccccgga
     1741 aaaaccccat agaaaatctg ccagagtagt aggggatgcc cttggcaaat atcatcccca
     1801 tggggataca tcggtatatg atgccatggt aaggatggcc cagaatttcg taatgagggc
     1861 ccctttagtt gatggccatg gcaacttcgg ttccatggac ggtgattcgg cggccgctat
     1921 gcgctatacc gaggttagga tgactccgtt ggctacggag ctattaaagg acataggcaa
     1981 agatacggtg gattatagtc tgaactttga tgatacctta aaggaaccgg atgtattgcc
     2041 gggaagattt ccgaacctcc tagtcaacgg ggcctcaggt attgccatag gtcttgccac
     2101 caacattccc ccccataact tggctgaggt tattgacggg gtaatcctcc agatggagaa
     2161 cccggatatt gccttggatc aactcttaaa tgtcataaaa ggccctgatt ttcccacagg
     2221 gggagttatt atagggcagg aggaaatcca aaaggcgtat gaaactggca gggggaagat
     2281 aatagtaagg gcaaaggttg atgtagagga tatagccggc ggtaaaaagt tattagtgat
     2341 aaaggagctc ccctatcagg taaacaaggc taccttgttg gagaggattc tcaggctcag
     2401 cgaagagaga aaaggggttt tgacgggaat tgcagatatc agggatgaat cggatcgtaa
     2461 cggggtacgc gctgtcattg aggtgaaacg ggacggggat gcagaaaaaa tcctcaacta
     2521 cctatataaa tattcggatc ttcaggtcac ctttggcgcg aatatggtcg ccatagccca
     2581 tggcagaccc cagcaattgg ggctgaagga tattctggga cattatatac agcaccagaa
     2641 ggaggttgtt accagaagaa ccaggtatga ccttgacagg gctaaggcca gggaacatat
     2701 tttagaagga cttatagttg ccattcataa catcgatagg gtaattgcta ttataagaga
     2761 atcaaagaat cctggtattg ccagaacaaa cctgatgacg gagtttgtgc ttacagaaat
     2821 tcaggcccag gctatcttgg atatgcgtct gcagaggctg accaacctag aggtggtttc
     2881 ccttgaaagg gagtatagag aagttaaaaa aaccattaaa aggctggagg tcatacttgc
     2941 atcggagaag cggctcattg ggcttataaa gaaggaacta aacgaagtta aggataagta
     3001 ctcaagcggg agaagaaccg gcatcatcaa agatgattcc gaggcggaga taagaactga
     3061 ggatttaatc catgtggaag aatgtgtagt tacccttacc agaaaccagg atataaaaag
     3121 ggtaccctta aaatccttca accgatccag caaggatgtg gaggtggtgg atacccgtga
     3181 gatggatttc atagaatttt tgatagactc cgagacggat cacagaatac ttttgctgac
     3241 ggacagggga aactcctata gcctggatgc gttggagatc ccggaagaaa aatggaggga
     3301 taaagggtca caattgatga gccttatcaa gggctttgac agaaacgaaa gaatcatagc
     3361 catgttttcc ataaaagaat tcgaagagga ccttttcatt caattctaca cggccatggg
     3421 gcaggtaaag aagaccagtc tggctgaata caatacccga aggagcagga tccaagcatg
     3481 cggactaaac caaggtgatc tatgtatcgg ggctgaactg gtagcccagg ataaggaaac
     3541 aattatagtc accgccgaag gtatgagcat aaggttcaac ggtgccgagg tgaatgctac
     3601 gggccgaaca gccaaagggg ttaggggtat tcagctgaag ggggatgaca gggtagtttt
     3661 tggcggtcag attgaaggag aaggcaatat aatcgtggtg acggaccggg ggattggcaa
     3721 gcggtcttcc gtagcggatt atcaagttca aggcaggggg ggtatcgggt ttaagacttt
     3781 gaccttttat aagaataggg ccaatggcag atacattttg ggggcctttt atgcaaaggc
     3841 gccctatgaa gtcatcctcc agcaaaccga cggtaccata gcccgtctta acaccaacga
     3901 cttgcccctt gctgcaaggg atggtagggg cagtaccata gttacggttg ctgaggataa
     3961 tatcgtggag tatgtctaca gaaactataa tgatgcatag cgggggtgtc tcggctttaa
     4021 acaaattaag tccgactgat ccgaaggccg gcttgatggc atagcttaaa tttcataatc
     4081 gttaacgaaa ggtccctcca tgggggactt tttttgtttt gattatcctt tatatcttga
     4141 ttatccctct tatagaagct atgaatagcg gacgggccca tcccctttat ttgggtattt
     4201 tactgaaaca attcttgatt ttgtcataag gatgctaata aataaataag tattagggag
     4261 gatgatacct atctgtaagc aaggacggag gtaatgcggt aatgcgggtt atatatattt
     4321 ccaagaaaaa gacggcaagg gtattattga ttttctgcct ggtcctagtt tcggttgcct
     4381 attccattaa cttcagggat aatgccgtag ctattttctt aaatactaaa cgggaattgc
     4441 ccatctactg tgtagagcgg gatgataaaa ggatatccat ttcctttgat gcggcttggg
     4501 ggactgagta caccgatgaa ttacttgaga tattggacga gagaaatata aaatgcacat
     4561 tctttttggt aggtttctgg atagatgccc atccggaaaa ggttcgacta atagcacaaa
     4621 aggggcatga aatcggtaac cattcaacca atcaccccca tatgagtaac ctgggcaagg
     4681 aacaaataat caaagaaatc gagactaccc aagaaaagat tgaggaattg gcgggtgata
     4741 gggctgtaaa gctcattaga cccccatatg gggattacaa taatttgctc ataagcacct
     4801 gtcgggaatt aggattttac cccattcaat gggatgttga ctccctggat tggaaggagt
     4861 tgggtgtgga tcatatgtat agacaggtca caggaaaggt taagccgggc tccattgtcc
     4921 tatttcacaa caatgctaag tacacgtcca ccgccttacc ttcaatactg gacaaatttc
     4981 ttgatgaagg gtatgagttt gtgccgatat cccaactcat atataaggat agctatgaca
     5041 tagaccatac gggaatgcaa aagaaaaagg gttcataagt catattggat ttcgcccatg
     5101 aataagaatt aaaataatga tatgagtgag gccacctatt actatatgtt ttacgacaaa
     5161 cattctacat tggtacatag gtgtcgtaaa acattatcct taattcgtat gttacgaaaa
     5221 tacattattt taacgtaaaa tcggataatt tagccaattt tacaatgaaa gatccttttc
     5281 gtaacggata taacataatc caggatgtta tcttaaacgt ggggatagag attatgctat
     5341 caaaagactc ggcataaata taggtacagg ggagaaagga tggagaggct ggatgactga
     5401 acatatagca gagaataata aggtagtggt agtgggcaaa gtgcacagtc ccatggaatt
     5461 tagccatgaa atatttgggg agaattttta tcagtttgat ctgtcagtac cccgtctcag
     5521 tgattatgtt gacttattac ccatcaccat ctccgagcga ttgctcgggg gaattgagct
     5581 ggctgagggg atggaaataa tcattgaagg tcagctcaga tcctataata aatttatagg
     5641 tggatacaac aggctaattc tgacggtatt cgcccgggaa ctactactaa aggatgaggg
     5701 gataaaaaac ccaaatcaga tttatttaaa cgggtttata tgtaaagagc cagtctacag
     5761 ggttacacct tttaacaggg agataaccga tattctttta gcagtaaata gggcatatgg
     5821 aaaatccgat tatattccca caattgcgtg ggggagaaac gcacgttttt ctcaacacct
     5881 gaatgtaggg gacaatataa ggatatgggg tagaattcaa agccggactt atcaaaagaa
     5941 gctcccaaaa ggagaggtat tagaaaaggt agcctatgag gtttcaattt caaaaatgga
     6001 agttgccaga gatgaaatgg aatcacaaac attggaaacc tgagcattgc tcaggttttt
     6061 atgttgtgaa tcggaattta gtatgataaa atgatagtgt aatgaattaa ccaaccgctc
     6121 tatgcagagc attcaaagga cgggtcggcg taagcggcta aaagggtaaa cgacggatgc
     6181 acgattgtat tttaatgtat tggttacggc tttgactggg gtgcaaaagg ttatttaaaa
     6241 aacctgagca aaatggtggc aaatatggtt ttgaatatcg attttacgag gggagattaa
     6301 tatgaaatac aaatcaaaac tgcggaacca acaggtggac gagctctttg acgccgtttt
     6361 ggaactgagg gacctggaag aatgctatcg attttttgaa gatatttgca ccataaacga
     6421 ggtaaaatcc ctggcccaga gactcggggt cgcaaagatg ctaagggcag ggaggacgta
     6481 ccaggacata gccgatgaaa caggcgccag tactgctact ataagcaggg ttaataggtc
     6541 attgacttat ggtgccgatg ggtataatat catattggac aggttattgg ataaataatt
     6601 ggacaccccc aaagggtgtc ttttaaataa gggaaagatt tatcatctat agataaattg
     6661 tgtgtttata ttgtttatct gtagtaaatt tcactgggag ttgaggcgtt tatggaactt
     6721 acaaggctgt tgaatccaca gcagaaaaag gctgtactcc atacgaaggg accccttttg
     6781 gtacttgcag gagcgggttc gggtaagacc cgtgttctta cgtatagggt ggcatacttg
     6841 gtagaaaaac taggggtaag ggcggggaat attttagcga ttacctttac gaataaagct
     6901 gccaaggaaa tgaatgaaag aatccatagt ctggtaggga gcaagtcccg ggatatatgg
     6961 atcagcacct ttcattccac ctgcgtcaga atactcagaa aagagatcga aagaataggc
     7021 tttacaagaa attttgttat atacgatgca gatgatcaac tgacccttat aagagattgt
     7081 ataaaagaac ttgatttaaa tgaaaagtat tttaatgcca aggatatacg ctttaggatt
     7141 ggacaactca aagatcagat aaaaagtccc gccgactata gaagggaaac gggaggccgg
     7201 tttcgggagg aaaaaatcgc tgacctatat gagttatatc agaataggtt agcaaagaac
     7261 aacgccttgg attttgatga tcttataaac aggaccttgg aactctttcg tttaagacct
     7321 gaagtcctgg attattacgc atccagattt caatatatct tggtggacga atatcaggat
     7381 accaaccacg cccaatatat gctgattaaa ttgttgtcca cgaagcatca gaatttatgc
     7441 gtcgtgggcg atgatgatca atccatatat ggctggagag gagccgatgt gaggaacatc
     7501 ctggaattcg aaagggactt tcccaatgcc gaggtaataa agctggaaga aaactacagg
     7561 tcatctcagg tgatcctgga tgcggccaat gaggtcatac gcaataacag cggccgaaaa
     7621 gaaaagagat tgtggaccca aaaaggtcag ggtgaaaaaa tagtagttta caaggtaccc
     7681 gatgagcacc gtgaagccga tatgatctgt cgacatatac acgggcatat agttgaggat
     7741 aataaagccc ccggagattt tgccgttcta tatagaacca atgcccaatc ccgggtaata
     7801 gaggatgccc ttatgcggta cggcatacca tatcgcatgt acgggggtct acggttctat
     7861 gacagaaagg aaataaagga tattatagcc tatcttaggg tggttgctaa cccggcggat
     7921 gatattagca taaaaaggat tataaacacc cccaggcggg ggataggcaa tgtaaccttg
     7981 ggtaacttgg aggaaatatc cataagagaa gacgagagta tcttcagcat aatcctggat
     8041 ttagaccaat atagggaatt tacccccaga atggggaaaa tgataaagga atttggggag
     8101 gctatatcaa acctcatagc catgaaggaa gtaatgcctt taacggaatt tattgaggct
     8161 ctattggagc atacaggcta tgaacaggcc atcaaggacg agggaacgct ggaatcagaa
     8221 acaaggttgg aaaacatcag agaattcatt tccgcagcta gggattttga aggggattat
     8281 gaggatgcgg ggctggttga atttttggag aatgtagcct tggtctccga tctcgataat
     8341 ttaggcgagg gccaatccgc ggttagtatg atgactatgc attctgccaa gggtctggaa
     8401 tttcctatgg tatttattgc cggtatggag gaatacctgt tcccgcactc cagatccatg
     8461 gattctcagc aggaaatgga ggaggaaagg cgactgtgtt atgtgggcat aaccagggcc
     8521 aaagaacggc tttacctgtc ccatgcggtc cggcgtaata tatttggtaa ctataaccgc
     8581 aatagcccgt cacgattttt agaggagatc ccccagacgc taatggaata ttctgatcaa
     8641 tctctggagg atatatgggg tacaagacag aaccggaata taacgggaaa tagatatgcg
     8701 ggaaacggac gggataaggt aggggtaggt gccctaggat cgcagccggg accttttcag
     8761 acatcaggga ctcctatggg tgtcaataag ggcgggacac atggtatccg aaacttttca
     8821 ctgggccaaa agatcgtgca taaaaaattt ggcgagggaa caatcgttgc tatgagcgga
     8881 agcggggatg atcaggagct aaagatagcc tttgaacaag gtggtattaa aagttttatg
     8941 gcaacctatg ctccgctaaa gaaattgtag gttggaggaa actgaaacat ggataaaagt
     9001 gatattctaa agaagattga aagcctgcgg gacgagataa gacaccataa ctatcaatac
     9061 tatatcttgg atcggcccat aatttccgat acagagtacg attctctaat tcatcgccta
     9121 atagatttag aggaagaaaa tccggaattt tattcccctg attcccctac ccagcgggtg
     9181 gggggaggat ctctagagga gtttggaaca gccgttcatt cctcgcctat gctcagcctg
     9241 gctaacgcct ttagccccga tgatttgttg gattttgatc gcagggtgag gggcatgtta
     9301 gaaaaggaag tggagtatat agccgaattt aaaattgacg gtctgtcggt ggttttagaa
     9361 tatgaaaacg gagtctttac aaggggtgct acaagggggg atgggctggt gggtgaggat
     9421 gttaccgaga atctaaggac agtaaggtcc atacccctga ggctgaataa gccgtactct
     9481 ttagaaaccc gcggggaaat attcatctcc aaagagcatt ttaagcagct taaccagagg
     9541 agagaactcc atggccaaca aacctttgca aaccccagaa atgcggcggc aggctctctg
     9601 agacagttag atcccaggat aaccgcatct aggcacttag atatcttcct attcaatctg
     9661 gaaagggtag aaggggtgga tattcatagt catttgcaga ccatggatgt catgaaagat
     9721 gcaggactaa agataagccc gtttctctac acaactacct ctatggaaga gataatcgaa
     9781 ttatgcatag aatggaccga taaacgacat gacctcccct tcgatatcga cggattagtt
     9841 ataaaagtca actccatcgg acagaggcag ctgttaggag ctacatccaa gactcccagg
     9901 tgggctattg cgtataaatt tcctgcacag caggaagaga ctcgcctgct ggaaatagag
     9961 gttcaagtgg gcaggacagg ggttctgacc ccaatagcca tcttagatcc ggtacagata
    10021 gcaggttcaa ctgtggcaag ggcaagcctc cataacgaag attatataat ggaaaaggat
    10081 atccgaatcg gcgattatgt tataatacaa aaggcggggg atatcattcc ggaagtggtc
    10141 agagtgcaaa aggataaacg gaacggagat gagaggaatt ttgagatgcc ggatcgttgt
    10201 cccgtttgcg gtgcacatac agttaggata gaaggggagg ccgccattcg gtgtaccggc
    10261 aatgcatgtc ctgcccaaca gaggcgactt gttatgcatt atgtatccag agacgctatg
    10321 gatataacgg gtttaggacc ggctttggtg gatcaattga ttgaaaacga cttgataggc
    10381 gatgcgggtg atctctatta tcttgattat aaccagttga ttcatcttga gcgtatgggg
    10441 gataagtccg ctgacaacct tttgaaggct atagagatca gcaagaaaaa cgagcttagg
    10501 cggctgatct ttgggctggg tatcaggttg gtgggtgtaa gggccgccca gcttatagcc
    10561 caaaaatttg ggcatctgga tgatcttcta agagccgggg aagacgattt attggcaatc
    10621 gatgagatag gtccaaaaat cgcccaaagt gtaacggcct tttttggtga ggaacaaaac
    10681 ctcaggctaa tcgataaatt aaggcgtgcc ggtgtcaact tctctcaaag tcaaagggct
    10741 tcggaccagg aaaatggtgc ccccacacat ggttggcggg ataagacctt tgttttgacg
    10801 gggaccttaa aggaatatac acgaaatgac gcaaaatccc taatagagga gcgggggggc
    10861 agggtcacag gcaatgtcag caagaaaaca agctatgtgc tagcggggga aaacccgggc
    10921 agtaagctga atagggcaag ggatttgggt attgttataa tcgatgagga ccaatttaat
    10981 tcaatgttgt gattattatc ctatgg
//
LOCUS       DASRNX010000199         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_39982,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000199 DASRNX010000000
VERSION     DASRNX010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39982"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..688
                     /locus_tag="VFD89_07935"
     CDS             47..688
                     /locus_tag="VFD89_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019241045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HZJ58149.1"
                     /translation="MKYKNIIFDLDGTIIDSQDGITRSAQYALKKYDIIVEDLEELVP
                     FIGPPLKDSFMDRYGFSERDAIQAVKYYREYFADHGGFANTLYPGIEDLLRKLHSEGR
                     MLFIATTKPTIYTDQILKGYELIDLFSYICGSNLDGTRTDKTEIIKTVIDESGIGIDD
                     TVMVGDRKYDIIGAQNNKIDSIGVGYGFGSEEELRAYNPTQYVKTVGDLYKAL"
     gene            complement(733..>2341)
                     /locus_tag="VFD89_07940"
     CDS             complement(733..>2341)
                     /locus_tag="VFD89_07940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZJ58150.1"
                     /translation="AMAEQLNQINQDMLKGGILNIESQREREIYFANILKSYPDAAMS
                     FIGLVDGSFYGAKRNLDGEIHVVRNNKSTDGASFYYSTNELGAGINLEERYPDFDPRT
                     RPWYTSAIEANKATYSEVYMHFVFQEPTITASRPIYDENDEIIGVFGVNYLISWMNDL
                     LSNLLIGKNGHVFVADSGGMLLATTLDIETYTADDDNLNLISIYESQNKFIEKSLMAF
                     DKNDTKERPSVNVAGKKYYTGVIDLDKSNLDWKLYFLLAEDDFLGNIKAAMIQAALLL
                     ALFVLISVAASSLVARRIAKPIEKLSLFAESLSDGMFKPVINDERIEEIYSLTNSFNV
                     NGEKLASLVADLEKQVAIRTNELECANRELEKLSFKDGLTGIYNRLKFDESYLLAWKT
                     ALRHGRRLGIFMLDIDYFKKYNDTYGHLKGDDCLRDIANELLRRLRRSTDFVARYGGE
                     EFVILIQDVDLDRIEGFAESIRSGIEGLNIEHSTSKFNHVTVSIGVSTMIPTADVEPT
                     ELIRRADLSLYRAKEEGRNRVHMDTPQ"
BASE COUNT          628 a          496 c          510 g          707 t
ORIGIN      
        1 gaatagcata agataaatac gactaatgac ctgggaggta actgtattga agtataaaaa
       61 cattattttt gatttagacg gtaccataat cgattcgcaa gacgggatta cgaggtcagc
      121 ccaatatgca ctaaagaaat atgatatcat tgtggaggac ctggaagagc tggttccctt
      181 tattgggcct cctttaaagg actcctttat ggataggtat ggtttttcag aaagagacgc
      241 tatacaagcg gttaaatact atagggaata tttcgctgat catgggggct ttgctaatac
      301 cttatacccc ggaatagagg atctactgag aaagctgcat tctgaaggcc gaatgctttt
      361 tattgctaca acgaagccta ctatatatac cgaccagata ttgaagggat atgagctaat
      421 cgatttattc agttatatat gcggctctaa tctggatggc actcggacgg ataaaactga
      481 gatcattaaa acagttattg atgaaagtgg gattggtata gatgatacgg tgatggtggg
      541 cgatcgaaag tacgacataa taggcgccca aaataacaaa atagattcta taggggtggg
      601 ctatgggttt ggcagcgaag aggagctgag ggcatacaat ccaacccaat acgtaaaaac
      661 agtaggggat ctatataaag ccctgtaagg gcataggaag tttcaccaat ttcaaggggg
      721 acgaagaagt ggttattggg gagtgtccat gtgcactcta tttctgcctt cttccttggc
      781 cctatatagg gataggtccg cccttctgat cagctctgtt ggttctacat ccgctgtggg
      841 gatcatagta gaaacaccga tactgacggt gacatggttg aatttggatg tggagtgctc
      901 tatattcagt ccctctatac cactccgtat gctctctgca aatccttcaa ttctgtctaa
      961 atccacgtcc tgaatcagta tgacaaattc ttccccgccg tatctggcta caaaatcagt
     1021 cgatctccga agccttctta gcagttcatt ggctatatcc cttaggcaat catccccttt
     1081 taggtgcccg taagtatcgt tgtacttctt aaaatagtcg atatccagca tgaatatgcc
     1141 taaccgtctg ccgtgacgca gagcggtttt ccaggccagg agataggatt catcaaattt
     1201 aagcctattg tatatccccg tcaacccatc cttaaaggac agcttttcca gctccctgtt
     1261 tgcgcattcc agctcgtttg tcctaatggc tacctgtttt tccagatcgg caaccaggct
     1321 tgctagtttt tcgccgttta cattaaagga gttggttagg ctatatatct cttctattct
     1381 ttcatcgttt atgacgggtt taaacatccc gtcggataaa ctctccgcaa ataggctcag
     1441 tttttctatg ggcttggcta ttcttctcgc taccagagag gatgcggcga cagatataag
     1501 gacaaacaat gccaacaata gtgctgcctg tatcatagca gctttaatat ttcctagaaa
     1561 gtcatcctct gccagtaaaa aatatagttt ccaatcgagg ttactcttat ccagatcgat
     1621 tacaccggta tagtactttt tcccggcaac attgacactt ggtcgctctt tggtatcatt
     1681 cttgtcaaaa gccattaagg acttttctat gaatttgttt tgggactcgt aaatacttat
     1741 aaggttaaga ttatcatcat ctgctgtata tgtttctata tccaaggtgg tggctaggag
     1801 catcccccca ctatccgcta cgaagacgtg cccgtttttg ccaatcaata ggttggataa
     1861 caggtcattc atccaggata tcagatagtt tacaccgaag acacctataa tttcatcgtt
     1921 ttcatcatat atcggcctgc tggctgtgat tgtaggttcc tggaagacaa aatgcatata
     1981 tacttcgcta taagtcgcct tgttggcttc tatggccgat gtataccagg gtctggtgcg
     2041 ggggtcaaaa tcgggatatc tttcttccag gttaatccct gcgcccaatt cattcgtaga
     2101 gtaataaaag gatgcaccat cagtggattt gttgtttctg actacatgga tttccccgtc
     2161 taagttgcgc tttgcaccat aaaagctgcc gtctacaagg ccgataaagg acatagccgc
     2221 atcgggatag gactttaata tgtttgcaaa gtagatctct ctttcccttt gggattcaat
     2281 gtttaagatg ccgcccttta acatgtcctg atttatttgg ttgagctgct ctgccatagc
     2341 g
//
LOCUS       DASRNX010000200         3632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_40046,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000200 DASRNX010000000
VERSION     DASRNX010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40046"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1041)
                     /locus_tag="VFD89_07945"
     CDS             complement(67..1041)
                     /locus_tag="VFD89_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58151.1"
                     /translation="MIQIDSISKSFRVSQRQAGFGQALKALFSREYNTIHALDDISFE
                     IKQGEMVGYIGPNGAGKSTTIKIMCGILRPDSGECMIGGRIPWKDRKNHAKEIGVVFG
                     QRSQLWWDVPVIDSFELIRDIYRVDMAKYRKNLDALVEQLDIGEIIKTPTRSLSLGQR
                     MRCEIAASLLHSPNILFLDEPTIGLDAVSKIAVRQFIRKLNTEKNTTVILTTHDMQDI
                     EALTERIILIGRGKILLDGDLQELKGRISTLKTLTVDYTGPAPVLEPGWTVINHREGH
                     IAIQFDSQKDSVSGVIAQVSKNTGVRDISVNGISAEEMVAGLYTEYRI"
     gene            1355..2230
                     /locus_tag="VFD89_07950"
     CDS             1355..2230
                     /locus_tag="VFD89_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HZJ58152.1"
                     /translation="MEKKLYTGLAMIILSLALLIILMPEDMEVINSTEIDIPEISGTV
                     ANLPWTEDEDFLKAQKNADAYVLMAGFCTVFENPQPNEEFNVHLAAKSVAGVVIQPNE
                     IFSQNNTIGPYDEEKGYKSGESYVGSEITTTIGGGVCKMATTLYNASVFSDLEVVERY
                     NHFMPVPYVPYGQDATVAYGSKDLKFKNITEFPILIWAEGIENRLYLALYGREKPSKV
                     EWSHKILSTLKASTINKINPNLPKGTQRVLVEGMDGAFVQSWVIVTDSDGSMRTKDMG
                     NSRYWPMPYIIETNK"
     gene            2336..3355
                     /locus_tag="VFD89_07955"
     CDS             2336..3355
                     /locus_tag="VFD89_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58153.1"
                     /translation="MDKDWLKISFIYVGTVIGAGFASGREIIEFFGIYGLKGIIGITI
                     SGILFSSLGCLFLLKIYKNRIRGFDDLVERAFGTKFGTALDIIMVVSLYTGFSVMVSG
                     SGAIFKEELEMSFDLGMIVMVASSFMVLLFRLKGLSFINTVLVPLLILGIVFTSLYLI
                     TDGQYNIYGNNGGINLTYKGNFFTSSLLYVGSNSLIIITVFSSLLPLISSRRAAVVGG
                     IVGGSILYVLGLSILSVMLLYYPEVSTLDIPMLKISDYIGSNYRKFYAIILWIAIFTT
                     AVANGSSFVNRFSNSRHKISIAAIFCLSSLPLAKLGFSKLIGLIYPIFGFIGFSILIV
                     ILVVL"
BASE COUNT         1009 a          767 c          778 g         1078 t
ORIGIN      
        1 caagggcggc ggctctgtac tgtaggccat ttataaatcg catctttaaa agtgcaaaat
       61 aagtactcat atcctatact ccgtataaag tcccgcgacc atctcttcgg cgcttattcc
      121 attgacggat atatcccgca cccctgtatt cttagaaact tgcgctatta ccccggatac
      181 ggaatccttt tgggaatcaa attgaatggc gatatggcct tcccgatgat ttatgaccgt
      241 ccacccgggt tctaaaaccg gtgccgggcc tgtataatcg acggtcagag ttttaagagt
      301 agatattctt cccttaagct cctgcaaatc gccgtctaat aatattttgc ccctgcctat
      361 aaggatgatc ctctctgtta atgcctctat atcctgcatg tcatgggtgg tgagaatgac
      421 cgttgtgttt ttttcagtat ttaatttcct tataaactgc ctgacagcta tcttcgaaac
      481 ggcatccagg ccaatggtgg gctcatccaa aaacagtata ttagggctat gaagcagcga
      541 agccgcaatc tcgcagcgca tcctttgacc cagactcaaa ctacgggtag gggtttttat
      601 tatctcgcca atgtcaagct gttccaccag ggcatcaagg tttttccggt attttgccat
      661 atccacccta tagatatccc gaatcagctc aaaactgtcg attacaggta catcccacca
      721 aagctgactg cgctgcccga aaacaacccc aatttctttg gcatggtttt tccgatcctt
      781 ccacgggata cggccgccta tcatgcattc accggaatcg ggccttagaa taccgcacat
      841 gattttaatg gttgtgctct tccccgcacc gtttggtcct atatagccga ccatttcacc
      901 ttgtttaatc tcaaagctaa tgtcatccag agcatgtatg gtattatact cgcgggaaaa
      961 tagcgccttt agggcttgcc caaacccggc ttgacgctgc gatacacgaa aggacttact
     1021 tatactatca atttgtatca aagcatgatc ccccccccga tactgcaata tttttagtaa
     1081 gcataataaa aatacgtatc cattaaaacc tggtctgttt tctattgttc atgtttttta
     1141 taatttttaa ttggccggtt atacttttta ttggtatctc aacagttgaa gggagtcaca
     1201 ggtcctttct tttaccatcc cataatatat taatttccct tatgttgtca aatgttatat
     1261 gcatgcaatc ccgtatagca aatgcatttt ttatcgtttc cggtaataga tatagtttag
     1321 ccaccaataa aaatttgcct tgacgaggga tagcatggag aaaaaattgt acaccggttt
     1381 agcaatgata attctgtccc tggcattact cattatactg atgccggagg atatggaagt
     1441 gatcaatagc acagagatcg atatacccga aatctccggt actgttgcca atttaccctg
     1501 gacagaggat gaagacttct taaaggccca gaagaatgcc gatgcatatg ttttgatggc
     1561 aggcttttgc accgtttttg aaaacccaca acccaatgaa gagttcaatg tccatttagc
     1621 tgctaagtcc gtcgccgggg tcgtaataca accaaacgaa atattttcac agaacaacac
     1681 aatcgggccc tatgatgaag aaaagggata caaatccggc gagtcatatg tgggctctga
     1741 gataaccacc actataggcg ggggcgtatg taagatggca accacattat ataacgcttc
     1801 tgtattcagc gatttggagg tagtagaaag gtataatcat ttcatgcccg taccctatgt
     1861 cccctatggc caggatgcga cggtagcata cgggtcaaag gatcttaagt ttaaaaatat
     1921 caccgaattt cccatactaa tttgggcaga gggtatagaa aacagacttt acttagcgct
     1981 atacggccgg gagaaacctt caaaggtgga atggagtcat aagatcctaa gcacgctaaa
     2041 agcctctacg ataaataaaa taaaccccaa tttaccgaaa gggacccagc gcgtattggt
     2101 tgagggtatg gatggtgcct ttgtacaatc ttgggttata gtaacagatt ctgacggaag
     2161 tatgagaacc aaggatatgg gcaatagtcg gtattggccg atgccctata ttatagaaac
     2221 gaacaagtag cccttgcggg tggaatcacg acaatccctt tatggattgt tggggggttt
     2281 ttaaaggcta ttgcatagac ctcccgctaa aatattgggt atggggggga atatgatgga
     2341 caaagactgg ttgaaaattt cctttatata tgtgggtacg gttataggag ccggctttgc
     2401 ctcgggaagg gaaataatcg agttcttcgg aatatacgga ttgaaaggga ttattggaat
     2461 aaccatatcg ggtattttgt tttcctccct gggttgcctg ttcctactta aaatatacaa
     2521 aaacagaata aggggatttg atgatctggt agagagagca ttcggtacga aatttgggac
     2581 ggcgcttgat atcataatgg tagtttccct gtataccgga ttcagcgtaa tggtctccgg
     2641 aagcggcgcc atctttaaag aggaattgga gatgtccttt gatctaggca tgatcgtaat
     2701 ggtggcctca tcctttatgg tgcttttgtt tcggctaaag ggtctttcct ttattaacac
     2761 tgtattagta cctttattaa ttctgggcat tgtgtttact tccctttatc taatcacaga
     2821 tgggcaatac aatatttatg gcaataatgg aggaattaat ctaacctata aagggaattt
     2881 ttttacctcc tcgcttttgt atgtgggctc taactctcta ataattatta cggtattctc
     2941 atcgttgctc cccttaataa gcagtaggag ggctgcagtg gttgggggca tagtaggtgg
     3001 gtccatattg tatgtcctgg gattatccat attatcggtt atgctgctat attaccccga
     3061 ggtctcgaca ttggatattc caatgctgaa aatatccgac tatataggca gcaattatag
     3121 aaaattctac gccatcatcc tgtggattgc catttttacc actgccgtag ctaacggttc
     3181 cagttttgtt aataggttct ctaacagtcg ccataagata tccatagccg caatcttttg
     3241 cttgtcctcc ctgcctttgg ccaaattggg cttctccaaa ctaataggcc tgatataccc
     3301 tatatttggg ttcataggat tctccatttt aatcgtaata ctggtagtgt tatgacggga
     3361 cagtaaaaat aattccagca aagtaagttt aggaaccgat acgaaacagg ctggacaatt
     3421 tccagtctct cgttataaaa tcaaaaagaa ggttcccccg gatccattac tgcttggaga
     3481 cagtattgga tatcagatat tccaatgcct tccaaaggac agctactgcc ctttagaaga
     3541 taacgggcgt gtattttata tttgaagaat tctatggaaa tcacattgtg aagaggctta
     3601 gaacctgtta ataaagcgga ggataggccg aa
//
LOCUS       DASRNX010000201         9025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_196846,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000201 DASRNX010000000
VERSION     DASRNX010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196846"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..4151
                     /locus_tag="VFD89_07960"
     CDS             246..4151
                     /locus_tag="VFD89_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HZJ58154.1"
                     /translation="MSVLLIEQTQLFDDIDEVIDILRREDRTARPPSFLWSKVSKRVH
                     GLLLQMVAKELGLPNPRFIASKDLEKHQFEFIGGHPLNGLYSYYKNNFPRKKGVHILI
                     NVCNELGIPEVSVDEWVAFISNNNIFSWKNISRPARKEILLKACDELDYAHPIFFRNE
                     DFSVRFKFLNERTLTGFYNYYKNKYDDIRGSTIINMCNDLGIEISEEDWISYITDPSI
                     SIFWDVVPHDIIRSILFQVAKMLGYENPRMMSTDDLKVSIPFLKGMSLYSFASRFFKL
                     DRGGISSIDYICDVYGVPELGYDEWISLISYQQNFKWESVPEPYIGRILQEKAKEMGK
                     SNPRLLKHEDLNTPTAFINNKTLASLHAPYAQRAAKMGMDTISYICGICGISPLSMEE
                     WLKLLKEPDIKIPWESMPDDIKKGIIIRAARELGLRNPRFMGYKDFCNTRFKFLNGKT
                     LGGLYYHYSSRDREQDVPVVQFMCNELRVDELSTSEWIDMIANSNICRWESVPLRVQR
                     EIIMRAAVEMGIFHPRLMGSKDLDSVRIKFLNNKTLAGLYSHYSTKLKADDTRINDLI
                     FDTLNIPQIDINPKTGRLTTANSVGHRKYFDSLANIREFFNTYLKHFDLGSLLRKHVM
                     LKNARKAGIYRRGLVTILAPLNRREYVDFVFEILKNTPRDSFGLRKPRGWGDYRLLKS
                     DLYKLADIQEELDRGISKIEFANPHLVDVRDSQDVIRELTMYMDILDIKIKKFHKIQC
                     VYLISYRDSYIFSMPISFFPGEVLTANAGWEFEVVECRDKKSNDGYIVELKALDNPTE
                     DQISQITTLARDSNSTILMSYLDRVIGAIERGTLSPLLKTVLALEKEQPLKPEDIPVL
                     PDEAYYNKDLVRNSAQKQAVALAQSMDGTNNALTIVQGPPGTGKTTLIKEIALQYYYQ
                     GKDVLILAKTNIAVDNVLDKLMEDKVRVLRTGNNIEAKSDLPYVYTVSTSNPTYTGLL
                     KGKNSIVLGTPLGYYLDRNISKNYFDVLIIDEASQMDVPESLFSMQFSNKCVIIGDHM
                     QIPPFPIQNEILLEYDPHISLNTSEMLQRSLFENLITDPYRYNSVFLDINYRTQDPLM
                     ISFISDLIYDGKLSANLDAEYYQVPPSIRRRKFSAAPIEIIDTSELVGPKERMETEVN
                     STFYNLTEAMISVSKVVDLLKEGEKLSDICIITPYKAHAEKLREVFEEHSRYFRGYSQ
                     GISKFIEQNIYTIDSFQGREQDNVIINWVRSNYVSPGTTTRTGFLKDYRRTNVALSRG
                     RKRLILIGDFETLTKSENMKVQYIFSKIKNTEIENKFVI"
     gene            complement(4156..5187)
                     /gene="tsaD"
                     /locus_tag="VFD89_07965"
     CDS             complement(4156..5187)
                     /gene="tsaD"
                     /locus_tag="VFD89_07965"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZJ58155.1"
                     /translation="MKSIAEKEEVIILGIETSCDETSAAVVRNGREVLGHKIASQIEL
                     HRKYGGVVPEIASRKHVEVINPLIDETIEEAGMSLREMDAIAVTYGPGLVGALLVGVA
                     TAKALAYGLEVPLIGVHHIEGHISANYIAHGDLAPPFLCLVVSGGHSHLVWVKGYGDY
                     EILGQTLDDAAGEAYDKVARTLKLGYPGGPVIDRLAQKGDPHFVEYPRPYMKDPHFNF
                     SFSGLKTAVINHLHSMDQRGQDYRPEDIAASFQQAVVDVLVDKTVKAAEDINAQKIVL
                     AGGVAANSGLRSRIKDVCREKGIGLYYPNPLLCTDNGAMIASAGYYKLRRGIISDLTL
                     NAVPGLLLA"
     gene            complement(5467..6183)
                     /locus_tag="VFD89_07970"
     CDS             complement(5467..6183)
                     /locus_tag="VFD89_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HZJ58156.1"
                     /translation="MGHFKAIWIIRVVGVLAVSLLIIGMFLTVIDWTAFDLNFYRREY
                     KKLNIPQSTGMGQGDLLKATEGLLDYVRGRRGDLMISAPVHGQERQVFNQREIDHMVD
                     VKDLFASGFRLRWASLGTFACLIALIIYFRGRKAARDLAISYLIVLSILIILGLILLI
                     FILIDFTTVWDQFHYTFFTNDLWLLDPATDILIQMVPEQFFFDMVVRILGTFGSILVI
                     IAIGAVMALSKTGRTKVGMK"
     gene            complement(6232..6660)
                     /gene="rimI"
                     /locus_tag="VFD89_07975"
     CDS             complement(6232..6660)
                     /gene="rimI"
                     /locus_tag="VFD89_07975"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZJ58157.1"
                     /translation="MKRGDIRDVLNIEKICFSTPWSYEAFRAELEGNHCARYVVAEQE
                     SMVKGYGGMWVILDEAHITNVAVHPHYRGIGIGEAIMRGLIDTAANLKLGSMTLEVRV
                     SNTIAQNLYKKLGFAGVGIRKGYYTDDGEDALIMWKERIS"
     gene            complement(6698..7426)
                     /gene="tsaB"
                     /locus_tag="VFD89_07980"
     CDS             complement(6698..7426)
                     /gene="tsaB"
                     /locus_tag="VFD89_07980"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZJ58158.1"
                     /translation="MDRYMMVLAVESSGTVAAVAVAKGEKLMGEFFLDHRRTHSQQLM
                     PLIEGLLNSLDIGLRDMDVFAVSKGPGSFTGLRIGIATVKALAQAHDRPVIGVPTLDG
                     LAHNLKAREGLVCPIMDARREQVYTSVYRSGGDDNHLGKGFERLDEYMALPVTELIDK
                     LMVYNEPVLFGGDGIIPYWGIIEDRMGTKAHKAPISLMMQRASSVACLALEAAQSGRT
                     QNYTQLVPFYLRKSQAEQRYGLKG"
     gene            complement(7411..7887)
                     /gene="tsaE"
                     /locus_tag="VFD89_07985"
     CDS             complement(7411..7887)
                     /gene="tsaE"
                     /locus_tag="VFD89_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZJ58159.1"
                     /translation="MEKMIEIITFSEKDTLELGKKIGTSARSGMIILLKGDLGTGKTV
                     LTRGLARGLGIEGPITSPTYTLIHEYQGRLPLYHFDIYRLNDSDEMYDLGYDEFFYGR
                     GVTVVEWPERMGDLLPHEYLGITIEKSPGDDADKRIIRIIPVGKVYEEFKEGWIDI"
     gene            complement(8116..8748)
                     /locus_tag="VFD89_07990"
     CDS             complement(8116..8748)
                     /locus_tag="VFD89_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HZJ58160.1"
                     /translation="MVEQKSIKKQMLSKKVFSLNGIVKVGLLSAIAFVLMLLEFPMPL
                     FPAFLKMDISDLPALIGGFALGPMAAVIIEFIKNVFHIIFKNDGTGGVGNLANFIVGI
                     SFAVPAAIVYIRNKTRKNAVIGMALGTLLLVIFSALANYYVLIPFYTKLYFEGNLENL
                     LAVMAMANKNITDVALYVLYAVMPFNLIKAFVISLITLLIYKRVSPILQK"
     regulatory      complement(8918..>9025)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         2514 a         2177 c         1939 g         2395 t
ORIGIN      
        1 gtatataata ttaaatggga atttttattt atggttttta gctgttgctg tatagaaaag
       61 taagtaacgt gaatatattt tgttagcttc atatgttgta ccggttagat acgccggtaa
      121 tggctattga acgctacttt aaatagctca ttacccttat cacacagtaa aataatgggc
      181 tggcacaaca atttaagttc ctttatgata atttttaaga tcagattttg agaggagagg
      241 cacatttgag cgttttgttg atagagcaaa ctcaattatt tgatgatata gatgaggtta
      301 tagatatttt gaggagagag gataggacgg ctcgcccccc ttcttttttg tggagcaaag
      361 taagcaaaag ggtacatggg ctcctgcttc agatggtggc caaggaatta ggcctaccaa
      421 atccccgctt tatagcgtct aaggatctgg agaagcacca gtttgaattc atcggcggcc
      481 atccgttaaa cgggctctat tcatattata agaataattt tcccaggaaa aagggtgtcc
      541 atatattgat caatgtctgc aatgagcttg gtatacccga ggtcagcgtc gatgaatggg
      601 ttgctttcat tagcaacaac aatatttttt cctggaagaa catctccaga cctgccagga
      661 aggaaatcct cctgaaggcg tgcgatgagc ttgattatgc ccatcccata ttttttagga
      721 atgaggactt ttccgtgagg ttcaagttcc taaatgagcg gacccttacc ggattttata
      781 attactataa aaacaaatac gatgatataa ggggcagcac tataataaac atgtgcaatg
      841 atctggggat tgagatttcc gaagaggatt ggatatccta tataaccgat ccatccatca
      901 gcatcttttg ggatgtggtt ccccacgata ttatccgtag catactcttt caagttgcga
      961 aaatgctggg ctatgaaaat cccaggatga tgtcaacgga cgatctcaag gtaagtatcc
     1021 catttctaaa gggtatgagc ctgtatagtt ttgcttcccg attcttcaag ctggatcggg
     1081 gcggaataag cagcatagac tatatatgcg atgtctatgg ggtgccggag ctgggttacg
     1141 acgaatggat cagcctaatt tcctaccaac agaattttaa atgggaatcc gtacccgagc
     1201 cttacatcgg ccgcatcctc caggaaaagg ccaaggagat gggcaagagc aatcccaggc
     1261 ttttaaagca tgaggattta aataccccca ccgcctttat aaataataag actctagcca
     1321 gcctccatgc cccgtatgct cagagggccg ccaagatggg gatggatacc atatcctata
     1381 tttgcggtat ctgcggcatt tccccgctat ctatggaaga gtggcttaag ctccttaaag
     1441 agccggatat aaagatccct tgggaatcca tgcccgacga cataaaaaag ggcataatca
     1501 tacgtgcagc aagggaattg ggcctacgta atcccaggtt tatgggatac aaggattttt
     1561 gtaacaccag attcaaattt ttaaacggca aaaccttggg cgggctctac tatcactaca
     1621 gctcaaggga tagggaacag gatgtcccgg tagtccaatt catgtgtaat gagttgaggg
     1681 tcgatgagct tagcacaagc gaatggatag atatgatcgc aaattccaac atatgccgct
     1741 gggagagcgt ccccttgagg gtccaacgag agattataat gagggcggct gtagagatgg
     1801 gtattttcca tccccgattg atgggctcaa aggaccttga ttccgtaagg atcaagttct
     1861 taaacaacaa gaccctagcg ggattatatt cacattacag cactaagcta aaggctgatg
     1921 ataccaggat aaacgacctt atttttgata ccttaaatat accccaaata gatataaatc
     1981 caaagaccgg tcggttaacc actgccaaca gcgtgggaca ccgtaagtac tttgacagcc
     2041 tggcgaacat aagggagttt tttaacacct atctgaagca ctttgattta gggagccttc
     2101 tgagaaagca tgtaatgctg aagaacgcac gcaaggccgg tatctatcgc aggggactgg
     2161 tcaccatatt ggcgccctta aaccgcagag agtatgtgga ctttgtattt gaaatactga
     2221 aaaacacccc cagagatagt ttcggccttc gaaagcctag gggatgggga gattacaggc
     2281 tgctaaaatc cgatctctat aagctggcgg atatacagga ggaattggat cgcgggataa
     2341 gtaaaatcga atttgcgaac ccgcatcttg tggacgtaag ggatagtcag gatgtaatcc
     2401 gtgagcttac gatgtatatg gatatcctgg acataaagat taaaaagttc cataagatac
     2461 aatgcgttta cctgatatcc tatagggatt catatatctt tagcatgcct atatcctttt
     2521 tcccgggaga ggtgctcact gcgaatgcgg gttgggaatt cgaggtcgtc gagtgccggg
     2581 acaaaaaatc caatgacggc tatatagtcg aattaaaagc tctggacaat ccaactgaag
     2641 atcagatctc ccagattact acccttgccc gggattccaa tagcaccata ttgatgtcct
     2701 acttggacag ggtcatagga gccatagagc ggggcaccct ctcgcccctt ttaaaaacgg
     2761 tgttggccct tgagaaggag cagcccttaa agcctgagga tatccccgtg ctcccggatg
     2821 aagcctatta caataaggat ctggtccgta attcggctca aaaacaggct gttgccctgg
     2881 cacagagcat ggatggaact aataatgcgt tgaccatagt ccagggaccc ccgggtaccg
     2941 gaaagaccac ccttataaag gagatagccc tccaatacta ttaccagggt aaggatgtat
     3001 tgatactggc caagacgaat atcgctgtgg acaatgtctt ggataagctt atggaggata
     3061 aggtgagagt cttaaggacg gggaacaata tagaagcaaa atccgatctg ccctacgtct
     3121 acacggtctc cacctctaac cccacctaca ccggcctttt aaagggcaag aacagcatag
     3181 tccttggcac tccccttgga tattacctgg acagaaatat ctcaaagaat tattttgatg
     3241 tgctcatcat cgatgaggct tctcagatgg atgttccgga aagcttgttt agtatgcagt
     3301 tttccaataa atgtgtgatt attggggacc atatgcagat accgccattt cccatccaga
     3361 atgagattct tttggaatac gatccgcata tcagtctcaa taccagcgag atgctgcaga
     3421 ggagcttgtt tgagaatctg ataaccgatc cctataggta caacagcgtt ttcttagata
     3481 tcaactaccg tacccaggat cccttgatga tatcctttat ctccgatctg atctatgacg
     3541 gaaagctatc cgccaacttg gatgccgaat attaccaggt ccccccgtcc ataaggagga
     3601 gaaagttctc cgccgctccc atcgagatca tagatacctc tgagctggtg gggcccaagg
     3661 aaaggatgga gaccgaagtc aattccacct tctataacct tacggaggct atgatatccg
     3721 tcagcaaggt ggtggatctc ctaaaggagg gggaaaaatt aagcgatatc tgtatcatca
     3781 cgccatataa ggcccatgct gaaaagctca gggaggtctt tgaggagcat tcaaggtatt
     3841 tccgaggcta cagccaaggt atttcaaaat tcatagagca aaatatctat accattgact
     3901 ccttccaggg aagagaacag gataacgtca tcatcaattg ggtcaggagt aactatgttt
     3961 cgcccggtac caccacccgg acgggttttt tgaaggatta tcgccggacc aacgtagccc
     4021 tgtcccgggg cagaaagagg ctcatcttga taggggattt tgagaccttg acaaaatccg
     4081 agaatatgaa ggtgcagtat atcttcagca agataaagaa taccgaaata gaaaacaagt
     4141 tcgttatata gaatcctagg caaggagtag acccgggact gcgtttaagg ttaaatccga
     4201 tataatgccc cgccttagct tataatagcc tgcagatgcg atcatcgcac cgttatcggt
     4261 gcaaaggagg ggatttggat agtagagccc tatccccttt tctcggcaga catccttaat
     4321 ccgggatctt aagccggaat tggctgccac cccgcctgcc agaactattt tttgggcatt
     4381 aatatcctct gccgccttta cggtcttatc caccaataca tccaccaccg cctgctgaaa
     4441 gctggccgct atatcctctg ggcgatagtc ttggccacgc tgatccatgg agtgcagatg
     4501 gtttattacg gcggtcttca ggccgctgaa gctaaagttg aaatgaggat ccttcatata
     4561 tggtctcggg tattctacaa agtggggatc acccttttgg gctaatctgt cgattacagg
     4621 acccccggga taacccagct ttagggtcct cgccacttta tcataggcct cccccgctgc
     4681 atcgtctaag gtctggccca gtatctcata atcaccatac cccttcaccc ataccagatg
     4741 gctatggccc ccggagacta ccaagcacaa aaatggaggt gccaaatccc catgggctat
     4801 gtagttggcg cttatatgcc cttctatatg atggacgcct attagcggga cctccaatcc
     4861 ataggccaat gccttggcag tagcgacgcc gaccaacaat gcgcccacca atcccggacc
     4921 ataggtaacc gctatggcgt ccatttccct gagggacatt ccggcctctt caatggtctc
     4981 atctatcaag gggtttataa cctcaacatg ctttcgagat gcaatttccg gcaccacgcc
     5041 gccgtacttt ctgtgaagct caatctgaga ggcgatctta tgccccaata cctccctgcc
     5101 gtttcttaca accgccgcag aggtctcgtc gcaggatgtc tcaattccca gaattataac
     5161 ctcttccttt tctgctatgc tcttcatctt ttccttcatt ctaatataat aactcacggt
     5221 ttcaccattt tcctatataa attgacgtgt tgaactatat gttttacgac aaagattcta
     5281 cattagtata cgggtgtcgt ataacattat ccccaattca tatgttacga aaaatacatt
     5341 gccttaacgc aagatcaggt aacttaactg attttgtatt gaaagatcat tttcgtagca
     5401 tatatagtta tcataaacaa tgttgtttaa ttgcaatata atcagcccct tacagatggg
     5461 gatattctat ttcatgccta ccttggtacg cccggttttg gacagcgcca tgacggcgcc
     5521 tatggcgatg attaccagga ttgacccaaa ggtccccaag atcctgacga ccatatcgaa
     5581 gaaaaactgt tccgggacca tctgaatcag gatatcagtg gccggatcca gaagccacag
     5641 atcgttggta aaaaaggtgt aatggaattg atcccatacc gttgtaaaat cgatcaggat
     5701 aaatatcaaa agtatcagac ccagaataat caatatggat aatactatga gataggaaat
     5761 cgctaggtct ctggcagctt tcctgcccct aaaatatata attagggcaa tcaagcatgc
     5821 aaaagtgccc aaagatgccc atctaagcct aaagcccgag gcaaacagat cctttacgtc
     5881 caccatatga tctatttccc tttggttgaa tacctgtctt tcctgcccgt ggacaggggc
     5941 cgatatcatt aaatcccctc ttctgcccct tacgtaatca agcaaccctt cggtggcctt
     6001 cagaagatcc ccttggccca tgccggtgga ttggggtata ttgagttttt tatactccct
     6061 gcggtaaaaa ttcaggtcaa aggccgtcca gtcgataacg gttaaaaaca tcccgataat
     6121 taagagggag accgccaata cgcccacgac tctaataatc catattgctt taaaatgtcc
     6181 catactatcc cccatcccct ttgaatctcc ggtccggtat cataaaaatc ttcaggaaat
     6241 cctttccttc cacatgatta gggcatcctc cccatcatcg gtgtaatagc cctttcgtat
     6301 tcccacgccg gcaaagccca gctttttgta caggttctgg gctatagtat tggataccct
     6361 gacctctaag gtcatgctgc ccaattttaa attagcggca gtatcaatca atcccctcat
     6421 aatagcctcc ccgataccga tgcctctata atggggatgg acagctacat tggttatatg
     6481 agcctcatcc aggattaccc acatgccgcc atagcccttg accatgctct cctgttccgc
     6541 tactacgtat ctggcacaat gattgccttc cagctcggca cgaaaagcct cataggacca
     6601 aggggtcgaa aagcatattt tttctatatt gaggacatcc cttatatctc cgcgtttcat
     6661 ggggcgtacc catatttttt ccacagcaca tctcccccta accttttagg ccataccttt
     6721 gttcggcctg ggattttctt aaatagaaag ggaccaattg tgtataattc tgggtgcgac
     6781 cggattgtgc cgcctccaga gctagacagg caacagacga ggcccgctgc atcataagac
     6841 tgatgggggc cttatgggcc tttgttccca tcctatcttc tattattccc caatatggta
     6901 taattccgtc tccgccaaat aacacgggct cgttatagac cataagtttg tcaataagtt
     6961 ctgtcaccgg aagggccata tactcgtcaa gccgttcaaa ccctttcccc aaatggttat
     7021 catcgccacc ggatctatag acagaagtgt acacctgttc tctcctggca tccatgattg
     7081 gacagacgag cccctccctg gcctttagat tgtgtgccag cccgtccaag gtggggacgc
     7141 ctatcacggg tctatcatgg gcctgggcta aggccttaac cgtggctatc ccaatcctca
     7201 gccccgtaaa tgagccaggt cccttggaaa cggcgaaaac atccatatcc cgaagcccaa
     7261 tgtccagact atttaaaagc ccctcaatca agggcatcaa ctgctgggaa tgggtccgcc
     7321 tatgatccag aaaaaactcg cccatcagct tttcaccttt ggctacggct acagcagcaa
     7381 ccgttccgga agactcaact gccaatacca tcatatatct atccatccct ctttaaactc
     7441 ttcgtatacc ttgcccacag ggattatcct aatgatacgc ttgtccgcat catcgccggg
     7501 ggatttttct attgtaatcc ctaagtattc atggggaaga aggtctccca tcctttcggg
     7561 ccattccact accgtaaccc cccggccata gaaaaactca tcgtacccta aatcgtacat
     7621 ctcatcggaa tcattcagcc tataaatatc gaagtgatat aagggcaatc tgccctgata
     7681 ttcatggatc aatgtatatg tagggctggt aatgggcccc tctatgccaa gccccctggc
     7741 cagtcccctg gtcaatacgg ttttgcccgt tcccagatcc ccttttagca gaattatcat
     7801 gcccgaccgg gccgatgtcc ctattttctt acctaactct agcgtatcct tctctgaaaa
     7861 agtaattatc tctatcattt tctccatagc cccatctata tggtttgtcc ccgtcaaaca
     7921 aaaatcataa ccattatatc cttatttata ctctctaaca agggattcat taaaaatggg
     7981 cgcataatcc gaatttgggg aataatattc caaccactta aattccaagg ccaggaacct
     8041 ataaactcgc tgtggagtcc gctaaaaaaa caccggggtc atattgcaga tcccggtgtt
     8101 tttattcgca tttacctact tttgcaggat tggcgacacc cttttatata tcagcagcgt
     8161 aataagggag ataacaaagg ccttaattaa gttgaagggc ataacggcgt acaggacata
     8221 gagggcgaca tccgtaatgt ttttattcgc catagccatg acagccaata gattttccaa
     8281 attgccttcg aagtatagct tggtatagaa gggaatcaga acataataat ttgccagtgc
     8341 agagaatata accaataata aggtgccaag ggccatccct atgacggcgt tttttcgggt
     8401 cttattcctt atatacacga ttgcggcagg tacggcaaag ctgattccga ctataaagtt
     8461 ggccagatta cctaccccgc cggttccgtc atttttaaat ataatgtgga acacattctt
     8521 aataaactcg attattacag ccgccatggg ccctaaggca aatcccccga ttaacgcggg
     8581 cagatcgctt atatccatct tcaaaaacgc cgggaataag ggcatgggaa attccaataa
     8641 catcaataca aaggctatag ccgataataa tccgaccttt actataccgt ttaatgaaaa
     8701 taccttctta gatagcatct gtttctttat ggatttctgt tcaaccatct taatcctcct
     8761 cttgattaaa atataatcct caatgacatc cattcccgtg acgggcttat gccctacagg
     8821 gttggactaa tacagtgtgg tcaaatgatt ttccacaaca aaatcccccc gaaacctcgg
     8881 ggggattatt caatgtcgca tgaaataaac atggtgacac caccttctct catccagact
     8941 atgactgtcg gccccagaat tgcgctgggt caatcctcta ttgaaggatt cgcgggctgt
     9001 accgccggta gggaatttca ccctg
//
LOCUS       DASRNX010000202         7821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_412825,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000202 DASRNX010000000
VERSION     DASRNX010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412825"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFD89_07995"
     CDS             <1..506
                     /locus_tag="VFD89_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZJ58161.1"
                     /translation="DMLMDYEIDLAIVEGVVNDTSINSLLLGTDYLVLVVSNNNPISK
                     KNMVTLNDLKKEKMILRLPGSGTRNLFIAHLESINMSIDEFNIILEVDNIATIKDLVR
                     RDFGASILARSACLDELNKGKITVLPIENLSMVRETSILYHKDFNHTDILQNITKIYN
                     ETARIYK"
     gene            complement(523..1023)
                     /locus_tag="VFD89_08000"
     CDS             complement(523..1023)
                     /locus_tag="VFD89_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58162.1"
                     /translation="MKKRSKMVALGGILSALCVICLYLAVYLPTNRLFFYSLASIFVS
                     FIVMESGIKSGWLFYIATSILSMLIIPNKIGIIPYLVFFGIYGIIKYYIESVNIIPIE
                     IVLKGTYFIAGEGILYILYSRILKIDITTRLPIYVVFGIFLVIFYLYDYVYSRLIAFY
                     RKRFMA"
     gene            complement(1036..1635)
                     /locus_tag="VFD89_08005"
     CDS             complement(1036..1635)
                     /locus_tag="VFD89_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HZJ58163.1"
                     /translation="MRDPYEVLGIRAGASEAEIKRAYKDLVRKYHPDQYAGNPLSSLA
                     EEKLKEINEAYDSLMKNKGGYTRGSDGDTGRDYNPYFAQVRQYILSGNIHEAERILNT
                     IGTRSAEWYFLKGHLELKKGNYHQGYENIKTAVQMDPGNMEYQQTLRNLMNQNTRAGQ
                     VYRTNNPSSAADDICKICTCLYAADCCCECMGSDLIACC"
     gene            complement(1632..2498)
                     /locus_tag="VFD89_08010"
     CDS             complement(1632..2498)
                     /locus_tag="VFD89_08010"
                     /inference="COORDINATES: protein motif:HMM:NF039826.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HZJ58164.1"
                     /translation="MFGYILPDKPELKIKEYELFKAYYCGLCKSISIHYGQIPRFALS
                     YDCAFLGLFLSSIHSSRGDVGPAKCITKPFRKIPVILDNPILEYAAAANVIFSYHKIE
                     DDIRDDRSYKYLPLILLLRSPYKRARNRVPALGAHAKFYLSELANLEGNNCASIDAAA
                     RPFANLLAKTFSGSGLFHDTKILEALEDFGYNIGKWLYTIDALDDMASDTKSGNYNPI
                     IEQFLKVGKDPDSSKQYIEDVEFILKYSLKRACIAYESLEIKSNKEILDNIIYGGMYK
                     KTDETLNKELEP"
     gene            3495..4028
                     /locus_tag="VFD89_08015"
     CDS             3495..4028
                     /locus_tag="VFD89_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HZJ58165.1"
                     /translation="MRRNSTKNMVLAGLFIALGIILPFFTGKVGELGRALLPMHIPVL
                     IAGFVCGWPYGLVVGLVTPFLSSFTTGMPAIPMATTMTFELAAYGLMTGLLYKLFPKK
                     DIYVYPALLISMLVGRVVLAVANIIMMGLSGGSYTMQMFIAGAFLNAIPGIIIQIVII
                     PIIIIALKRAGVVQDGR"
     gene            4018..4635
                     /locus_tag="VFD89_08020"
     CDS             4018..4635
                     /locus_tag="VFD89_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HZJ58166.1"
                     /translation="MVDKVRVKEGENCRNTIQVRYPGLMDRIDSLMGSGKPINIAIDG
                     NSGAGKSTLAEELGGIYDANIFHMDDFFLTPTLRTEERLREVGGNVDYLRFREEIIEG
                     LKTGQGFKYRPYNCQTMALEGDVAVVPRQLNIIEGVYSMHPTLTESYDLKIFLSVDSE
                     EQSRRILERNGAMMHKRFTDEWIPMEDEYFHKMGIRQECDLIFEV"
     gene            complement(4754..5164)
                     /locus_tag="VFD89_08025"
     CDS             complement(4754..5164)
                     /locus_tag="VFD89_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HZJ58167.1"
                     /translation="MKIVIIDGQGGRMGKMVVEQIKEALPGYELIAIGTNSIATAAML
                     RAGADFGATGENPVVFNCADADIIIGPLGIVVANALLGEVTPAMALAVGQSGAYKILI
                     PINKCNNVVVGIKELAYSEYVSLAIDEVKRIVEK"
     gene            complement(5311..6036)
                     /locus_tag="VFD89_08030"
     CDS             complement(5311..6036)
                     /locus_tag="VFD89_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZJ58168.1"
                     /translation="MSNDSNKQPKYRAHVPPCPGGTLYTIRAGDTLFTLAGRFNTTVD
                     AIMMANPGLDPMNLQVGRVICIPTAPQPGQCPGGFHYQIVAGDTFFNLARRFNTTVEA
                     LMAANPGVDPNRLMIGQVICIPVTPTPPCPGGTYQIRPGDTFFNIAMRFNTTVEALMA
                     ANPGVDPNRLMIGQVICLPPGTPGPIPCPGGTIYRVQQGDSLYLIGLRFGIPLDRLIA
                     ANPQLQDPSRLTIGQPICIPPRM"
     gene            6427..7461
                     /locus_tag="VFD89_08035"
     CDS             6427..7461
                     /locus_tag="VFD89_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HZJ58169.1"
                     /translation="MDKPLYDMTIQIPIIKESPSLAASGGGGSAESGGPSKSWQVTQT
                     GDTFLSMEREMESRTSLTLFYGHLQVLVISDSVAEAGLDKISDFFLRHREMRPKVDVY
                     IAEDSAKKILDVNPKVEDHAATHLKQLPINSDRNSRIVYRSDLGETMNCIRSGNDFVL
                     PKVEATKEEIKVKGGALFKRAKMVGWVDEIDIESINFIRNLYRRGVVVGNRPSDEPGI
                     ITLEVTKSKAKIKPMIIGNELNFDISFKIQGEYGGQVGFATDKQADNIFIEKCEKEFS
                     RVIEEQCKKTIKKMQREYNVDVFQFNKILRTQKPAYWEEIKENWDEIFPNARINLKVE
                     TRIKVTGAMK"
BASE COUNT         2243 a         1579 c         1853 g         2146 t
ORIGIN      
        1 atgatatgtt gatggattat gaaatagatt tagctatcgt agagggagtg gtcaatgaca
       61 caagtatcaa ttcactattg ttgggtacgg attatctggt attggtagtc tccaataata
      121 atccgatttc aaagaaaaac atggtgacct taaacgatct aaagaaggaa aagatgatcc
      181 tgagactccc gggatccgga acaagaaatc tttttattgc ccacctcgag agcataaaca
      241 tgtccatcga cgaattcaat atcattttgg aagtcgacaa tatcgctact ataaaggatc
      301 tggtccgaag ggattttggg gcatctatcc tggcccgaag tgcatgtctg gatgaactca
      361 ataagggtaa gatcaccgta cttcccatag aaaacctcag tatggtcagg gagaccagca
      421 tactatatca taaggatttt aatcatactg atatattaca gaatataacc aagatatata
      481 acgaaacggc aaggatatat aaatagggta tccgtatcct gtttatgcca taaaacgctt
      541 cctgtagaag gctattaatc tcgaatagac gtagtcatag aggtagaaga tgaccaaaaa
      601 gatcccgaat acgacgtata tgggcagcct tgtggttata tctattttaa gtatccgaga
      661 ataaaggatg tataaaatac cctcgccggc tatgaagtag gttcccttta atacgatctc
      721 tataggtatg atattcacgg attctatata gtattttata ataccgtata tcccgaagaa
      781 tactaagtat ggtataattc ctatcttgtt aggtatgatg agcatggaca ggatggatgt
      841 ggctatataa aagagccagc cgctctttat accggattcc attactataa aggaaacaaa
      901 tatggaggcc aatgagtaga aaaacaacct gttggtggga agataaacgg ccagatacag
      961 acatatgaca caaagggcgg ataatatgcc tccgagggcg accattttac ttctcttttt
     1021 caaggggttt ccctcctaac agcaggcgat aaggtcgctg cccatacatt cgcagcaaca
     1081 atctgcggcg taaaggcagg tgcagatctt gcaaatatca tcagcagcgc tggatgggtt
     1141 gttggtcctg taaacctggc cggctctggt attctgattc atcagattcc ttaaggtctg
     1201 ctggtattcc atatttccgg gatccatctg taccgccgtc ttaatgtttt cgtagccttg
     1261 atggtagttg ccttttttca gttccaaatg tcccttaaga aaataccact cggcagatct
     1321 ggtgcctata gtatttaata ttctctcggc ttcatggata ttaccggaga gtatatattg
     1381 cctgacctga gcaaagtaag ggttataatc ccggccggtg tccccgtcgc ttcctcgggt
     1441 atagcccccc ttattcttca taagtgaatc gtatgcctcg ttgatctctt ttagcttttc
     1501 ctccgccagg gacgaaagcg ggttacccgc atattgatcc ggatggtact ttcttactaa
     1561 atccttgtac gcccttttta tctcggcttc cgaagctccc gctcgtatgc ccaggacctc
     1621 atatggatct ctcatggttc caactccttg tttaaagtct catcggtttt cttgtacatc
     1681 ccgccatata ttatattatc caggatctct ttgttgcttt taatctccaa ggattcgtag
     1741 gctatacagg ccctcttgag ggaatacttt aagatgaatt ccacatcctc aatatattgt
     1801 ttcgaactat cgggatcttt acctactttt agaaattgtt ctattatcgg gttgtagttt
     1861 ccgcttttag tatccgaggc catatcatcc aatgcatcta tggtatagag ccatttgccg
     1921 atattatacc cgaaatcctc taaggcctct aggattttcg tgtcgtggaa taggccactg
     1981 cccgaaaagg ttttagccag caggtttgca aagggccggg cagcggcatc tatggatgcg
     2041 caattattgc cttccagatt cgccagctca gaaagataga attttgcatg ggcacccaga
     2101 gcaggcaccc tatttcgggc tcttttatat ggtgagcgca gtaataaaat caaaggcaga
     2161 tacttatagc tcctatcgtc cctgatgtca tcttcaatct tatgatagga aaagattaca
     2221 ttggcagctg ctgcatattc taaaattggg ttatccaata taaccggaat tttcctaaag
     2281 ggcttggtaa tgcatttagc cggccctaca tccccacggg aagaatggat tgaagataaa
     2341 aacaaaccca aaaaagcaca atcatagctc aaggcaaaac gcggtatctg cccataatgg
     2401 attgatatgg atttacataa gccgcaataa taggccttga acagttcgta ttcctttatt
     2461 tttagttccg gcttgtccgg aagtatatat ccaaacatat ctaaacccta agtcttccct
     2521 cttctataac atcacaaata attgtcaatg cggctataat gcgactcgca ggttctggtg
     2581 aaaccgacga tttataccgc tgtctatatg ttttacgcca aacattctac attggaacac
     2641 aggtgtcgta aaacattatc cttaattcgt atgttacaaa aatacattat tttaacgtaa
     2701 aatcggataa tttagccgat tttacaatga aaggtctttt tcgtaacata tatagctact
     2761 tgattctgat atacatgata tcagaaatat acacccaata caataaaaca agtagagatg
     2821 aaggagctta accggttgtg taggattgtc ctatgggcgt gtattttata tttgaagaat
     2881 tctatggaaa tcacattgtg aagaggctta gaacctgtta ataaagcgga ggataggccg
     2941 aatcactgtt tgagcgtagc gagtttgaga aggccccgga gcaaatttac aggttcttag
     3001 cctcggaact taagtgattg gaatattatt cttcaaattt atattatgca cccttgtcct
     3061 ataaatgaaa ttgacattaa tagaatatgt gctaaaatta ttataagggg ccggaaacat
     3121 gcccccactt ccaaaagtgg cgggtcccga ccagttaaaa caggtgtcgg ggatatatcc
     3181 tccgccttgc tggaacccat gtgctataat tgctgataca attgcttcca aagttatata
     3241 gataaacaat acgggaacta aacttatcga cgtgatgaat tttatagttg aagctgtttg
     3301 tctataagag ccggcagaag ccggtcaaaa cggcataatt ggcatagggg tttaatgcaa
     3361 aaacagtttt gcgagagaat caatataggc cttaatttaa gaatttttgt atatatacca
     3421 tgaaggtagc ggtcctttgc agaggggctt atcttatggt ttttttattt taatcaaaaa
     3481 ctggagggaa gtatatgaga agaaattcta cgaaaaacat ggtcttggcc ggtctgttca
     3541 tcgctctagg tattatactg ccatttttca cggggaaagt tggtgagttg ggacgggcgc
     3601 tattgcctat gcatattccg gtattgatag cgggctttgt ctgcgggtgg ccctatggtt
     3661 tggtggtagg tttggttacg ccctttctca gcagctttac aacgggaatg ccggctatac
     3721 ccatggctac tacaatgacg tttgaactgg ccgcttatgg ccttatgacg ggactattgt
     3781 ataaactgtt ccctaaaaag gatatatacg tatatcccgc cttgctcata tccatgctgg
     3841 tgggcagggt tgtactggct gtagctaata tcatcatgat gggtcttagc gggggcagtt
     3901 ataccatgca aatgtttatt gcaggagctt tcttaaacgc cattcccggc atcatcattc
     3961 agatagtcat tatacctata ataattatag ctttaaaaag ggcgggcgtt gttcaagatg
     4021 gtcgataaag tccgggtgaa ggaaggggaa aattgcagaa atacaataca ggtgcgatat
     4081 ccggggctca tggataggat agactcgctg atgggatccg gaaaacccat aaacattgcc
     4141 atagacggca atagcggggc gggaaagagt accttggctg aagagcttgg ggggatatat
     4201 gacgccaata tcttccatat ggacgatttc tttcttaccc caacgctgag gacggaagag
     4261 agattaagag aggtcggcgg caatgttgac taccttcggt ttagggagga aattatagag
     4321 gggctcaaaa ccgggcaggg attcaaatac cgcccataca actgtcagac catggccctg
     4381 gaaggggatg tagccgttgt ccccaggcag ctcaatatta tagagggcgt gtatagcatg
     4441 catcctaccc ttacggaaag ttatgatctt aagatatttc tttctgtaga ttccgaggag
     4501 caaagccgga ggattttgga gaggaacggg gccatgatgc acaaaagatt tacagacgag
     4561 tggattccca tggaggatga gtactttcat aagatgggga ttagacaaga gtgtgattta
     4621 atctttgagg tataatttaa cccgctatgc cggtattgga ttttcaccgg tatagcgggt
     4681 taatctgttt tggtgaattt atctgtttta gtgggttcac ttattgtcac gggcttatcc
     4741 gttgtgccgc agattatttc tcaacaattc tttttacctc gtctatggcc aggctgacat
     4801 attcgctgta ggcaagctcc tttataccca ccactacatt attgcatttg ttaatgggga
     4861 tcaatatctt atacgcgccg ctctggccga cagccagtgc catagcgggg gtcacttccc
     4921 cgaggagcgc attggctacc acgatgccta agggaccgat gatgatatcg gcatcggcac
     4981 agttaaatac gacagggttt tcacctgtgg cgccaaaatc tgcacctgcc cttaacatgg
     5041 cggcagtggc tatgctgttg gtaccaatgg ctatgagctc atagcccggc agggcttcct
     5101 tgatctgctc gacaaccatc ttacccattc ttccgccctg tccgtcaata ataactatct
     5161 tcaaaatgtt accccctaca aaaatctgaa gaaattattt tcaagaaaaa taataggttc
     5221 tatctaggac ctataatagc atgatttcgg taatgaaagc aagcaaaaac aaggggcatg
     5281 tagggacccc ttgtcttgga caatcatggc ttacattctg ggcggtatac aaataggctg
     5341 gcctatggtt aagcgacttg gatcctgaag ctgagggttg gcagcgataa gcctatctag
     5401 gggaatgccg aatctcagac ctatcaaata gagggagtcc ccctgttgca ccctgtagat
     5461 ggtgccgccg gggcagggta tcgggccggg ggttccgggg ggcaagcaga taacctgacc
     5521 gatcatcagc ctattggggt ctaccccagg atttgcggcc atcaaagcct ctaccgtggt
     5581 attgaacctc attgcaatgt taaagaatgt gtccccgggc cttatctggt aggtgcctcc
     5641 gggacagggg ggagtaggag taacggggat acagataacc tggccgatca tcagcctgtt
     5701 gggatctact ccgggattgg ctgccataag cgcctcaaca gttgtattaa atcgtcttgc
     5761 cagattaaag aaggtatcac ctgctactat ctgataatga aatcccccgg ggcattgtcc
     5821 cggctggggc gccgtgggaa tacatattac tctgcctacc tgaagattca taggatccag
     5881 accgggattt gccatcatta tggcgtctac ggtggtatta aagcgcccgg ctaaagtaaa
     5941 tagcgtatcg cctgccctga tggtgtagag agtaccgccg gggcatggcg gaacatgggc
     6001 tctatatttt ggttgcttat tagaatcatt gctcacgcat cttcatcctt tctaatatcg
     6061 atatactata tattattaaa aagaaatttc tttgttacag ttatggcaca tttcgggttc
     6121 ccatagataa gatatatcag gcttaggctt aatttagtgg caagggcggg cggaccgccc
     6181 ctttattatt tttggacgag ttggaaacaa taagaggtat tacgactata caggggtgaa
     6241 aagaaaggtg tggagattct ccaaaaaggg agcaagcgga gggaaacgga tacagcaggc
     6301 aggatcggag caggaagaca accgggtcca aggggaggcc gggaacaata gcatcccaaa
     6361 aaccgagcgg gaacggagga aggggatacc gcaaaacagg cccagtttcg ccagatggat
     6421 ttgtccgtgg ataagccctt atacgatatg accatacaga ttccaataat taaggaatcc
     6481 ccgtctttgg cggcatcagg cggtggcggg agcgccgaaa gcggcggccc gtccaaaagc
     6541 tggcaggtaa cccaaaccgg agacaccttt ctcagtatgg aaagggagat ggaatccagg
     6601 accagcttaa ccctattcta tgggcatcta caggtattgg ttatatccga tagcgtagcc
     6661 gaggcagggc ttgacaagat atcggacttt tttctgaggc atagggagat gcggccaaag
     6721 gtagatgtgt atattgcgga ggattccgca aaaaaaatat tagatgtaaa cccaaaggtc
     6781 gaagaccatg ctgccaccca tctaaaacaa ctccccatca actcggatag aaactccagg
     6841 atagtctata gatcggattt gggggagaca atgaattgta ttcgctcagg gaatgacttt
     6901 gttttgccca aggtagaggc taccaaagag gagataaagg taaagggggg tgcgctgttt
     6961 aaaagggcaa agatggtagg ttgggtggat gaaattgata tagaatccat aaattttatc
     7021 agaaatctat atcgcagggg cgtggttgta ggaaacagac ccagtgatga accggggatt
     7081 atcaccttgg aagtgacaaa atccaaggcg aagataaagc ctatgattat tggaaatgaa
     7141 ttgaatttcg atatatcctt caaaatacag ggggaatatg gtggacaggt aggatttgcc
     7201 acagataaac aggcagacaa catctttata gaaaagtgtg agaaagaatt ctccagggtg
     7261 atagaggagc aatgcaaaaa gaccataaaa aaaatgcaaa gggaatataa cgtggatgtc
     7321 tttcaattca ataagatatt gcgtacccaa aagcctgctt attgggaaga aataaaggaa
     7381 aattgggatg agatattccc aaatgccagg attaatctaa aggtagaaac caggattaag
     7441 gtgaccgggg ctatgaaata gccattagca gagataagga aaggccgtag ggctgtaagg
     7501 aaagatcacg gccttcctat aataaaactg ttctagggat cttcttcgca atatacatcg
     7561 tcatgccgtg ggtcacatgg gccaatatta ggttcaccac agtgaattat gcctccgtca
     7621 acatccgtat ctatcagggt gataggataa atgctatctc ttaaaaagag aataaaaacc
     7681 gccttttttc ttttccgagc tggcgtcgtc atagatatct tctgtcatag gttcggggtt
     7741 ctccttctta atattgactg tttcctgagt ctcctttttt tttggtgcgc tatttgctac
     7801 atagtgagaa ctgaatcgga g
//
LOCUS       DASRNX010000203         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_305255,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000203 DASRNX010000000
VERSION     DASRNX010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305255"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFD89_08040"
     CDS             <1..527
                     /locus_tag="VFD89_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZJ58170.1"
                     /translation="LDSGKGILGDKLLPGGMLYFKIDDPLIRLTGEADEEDIERAIMK
                     KLKMDGLVLADVNLVREMDRDIDGASHIVPARINKNGKLGKSSAASMDQFDSLCSYSR
                     DLLIRMGQEIMEGNAYINPYRYKKMTACRYCPYSSVCQFDPSFQDNRYRLIKDMKEEE
                     VWSNMGDGRDDSGQ"
     gene            517..>3455
                     /locus_tag="VFD89_08045"
     CDS             517..>3455
                     /locus_tag="VFD89_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZJ58171.1"
                     /translation="MGSKIRWTREQSDAIRVKDSNLLVAAAAGAGKTAVLVERIIQRV
                     TDPKDPADIDRLLVVTFTNAAATEMKERIGDALSDALEDDPESEVLQRQLALLNRSSI
                     TTIHSFCLEVIKNNFHQLDIDPNFRIANQTEGLLLKLEALEELFEDRYLRDDMDGDFL
                     DLVESYSSNRDDKRLQDMVLGLYDFVQSHPWPKEWLHRHSQDFNIPDGTDLGDTPWGR
                     ELIESTRAEVMGLSESMSRAIDIIKGDPGLQPYLKNFTEEHSAIEDVLKGFHDGWEGI
                     YNCIKEVDFKRLSRCGKDADKDKQEQVKKIRDNAKAGIKRIQDRLFGIEPDQVIRDLR
                     SLYPKMKCLTGLVLELGERYQAKKRDRGLVDFNDLEHMCLEVLLEENEDGDRIPSEIA
                     LKLRERFHEVLVDEYQDSNLVQEVILTTVSRKDSQTPNMFMVGDVKQSIYRFRQARPE
                     LFLEKYASYSLEQGSRDRKIQLFKNFRSRKTIIDGVNFIFRQIMSQDVGELDYDDEEA
                     LNPGAVFREGEHGEPAEGEPIEFHIVDMKGDTPIEGGEYYQAMEYDYGEDGGDYGDGY
                     YGDDEEDGEGLSAGEPLDIIQTEARIIARRILEMVSPKGEGAPVRVFDKAKGDYRNAE
                     FRDIVILLRTTANWADTFQEELSIQGIPVYSDSGTGYFKTIEVQTMLSLLQIIDNPMQ
                     DIPLLAVLRSPIETWSPEELIDIRLTDRESTFYEALVKKSREGDSRLAQKARTFIEKL
                     NGWRDISQYLSTDELIWHLYGETGYYSCVGALPGGVQRQANLRILFERARQYEETSYK
                     GLFNFVHFIDRLKTSQGDMGSAKILGENENVVRIMSIHKSKGLEFPIVFVAGCGKQFN
                     LTDTTRNILFHHDLGLGPDYVDYDRRIIYSTLAKESLKYRITLETLSEEMRILYVALT
                     RARERLIITGAVKDLKREISKWADALSIEDLKLPGYETMRARRFLDWIGPAIIRHGDG
                     APLRQIL"
BASE COUNT          999 a          700 c         1001 g          755 t
ORIGIN      
        1 ttttggacag cggcaaggga atattggggg ataagctgtt gcccggcggg atgctgtatt
       61 ttaagataga cgatcccctg attcggttga ccggtgaggc agacgaggag gatatagaaa
      121 gggccatcat gaaaaagctg aagatggacg gactggtctt ggctgatgta aaccttgtaa
      181 gggaaatgga ccgggatatc gacggcgcat cccatatagt tccggcacgg atcaacaaaa
      241 acggtaagct ggggaagtct tcggcggcca gcatggatca gtttgacagc ctatgcagct
      301 acagcaggga tctgttaatt cgtatgggcc aagagataat ggaaggcaac gcctacataa
      361 acccctatag atacaagaag atgacggcct gcaggtattg tccctattca tcggtttgcc
      421 agttcgaccc ctcgtttcag gacaatcgtt atcggctcat aaaggatatg aaagaggaag
      481 aggtctggag caatatgggt gacgggaggg atgacagtgg gcagtaaaat aagatggaca
      541 agggagcagt cagatgccat aagggtaaag gacagcaacc ttttagtggc ggctgcggca
      601 ggggcaggca agacggcggt cctggtggag agaatcatac aaagggttac ggacccaaag
      661 gatccggcgg acatagaccg gttgttggtg gttacattta ccaacgccgc tgctacggag
      721 atgaaggaaa gaatagggga tgccctctcc gacgctttgg aggatgatcc tgaatcggaa
      781 gttctgcagc gtcagctggc ccttttgaac aggtccagta tcaccaccat acactctttc
      841 tgtctggagg ttataaagaa caattttcat cagttggata ttgaccccaa ctttcggata
      901 gcaaatcaga ccgagggcct gcttttaaag ctggaggcct tggaggaact attcgaggac
      961 aggtatctaa gggatgatat ggacggggat tttttggacc tggtagagag ttacagcagc
     1021 aaccgggacg ataaaaggct tcaggatatg gtgctggggc tctatgactt tgttcaaagc
     1081 catccctggc caaaggagtg gctccatagg catagccagg attttaatat ccccgatggg
     1141 acagaccttg gcgatactcc atggggcagg gagctcattg aaagcacaag ggcagaggtt
     1201 atgggactat cagaatccat gtcccgggct atcgatataa taaaaggtga ccccggtctg
     1261 cagccatacc ttaaaaactt tacagaggag cattcagcca tagaagatgt tttaaagggc
     1321 tttcatgatg gctgggaagg catctacaac tgcataaaag aggtggattt taaaaggctg
     1381 tccagatgcg gcaaggatgc ggataaggat aagcaggagc aggttaagaa gataagagac
     1441 aacgccaagg ccggaattaa gaggatacaa gacagattgt ttgggataga gcccgaccag
     1501 gttataaggg atttgaggag cctttatccc aagatgaagt gccttacagg ccttgttttg
     1561 gaattggggg agaggtacca agcaaagaag agggacagag ggctggttga ctttaatgat
     1621 ttagagcata tgtgcctaga ggttttactt gaagaaaacg aggacgggga cagaatccct
     1681 tccgaaattg cccttaagct tcgggaaaga ttccacgagg tcttagtgga cgaataccag
     1741 gacagcaatc tggtgcaaga ggtgatcctt accaccgtat ccagaaagga tagccaaacc
     1801 cctaacatgt ttatggtggg ggacgtaaag cagagcatct atcgctttcg ccaggcaagg
     1861 ccggagctgt ttttggaaaa gtatgcctcg tattcgctgg agcaggggtc tagggatcga
     1921 aagatccagc tctttaaaaa ctttaggagc agaaaaacca tcatagatgg ggtcaacttc
     1981 atattccggc agataatgtc ccaagacgta ggggaactgg attatgacga tgaggaggcg
     2041 ttaaatcccg gagccgtctt tagggaaggt gaacacgggg aaccggcgga aggcgagccc
     2101 atagaatttc atatagtcga tatgaagggc gataccccta ttgaaggcgg cgaatactac
     2161 caagccatgg aatatgacta tggggaagat ggtggtgatt atggggatgg gtactatggt
     2221 gacgatgaag aagacggtga agggttatca gcaggagaac ccctagatat tatccaaacg
     2281 gaggccagga taattgcccg aaggatattg gagatggttt cgccaaaagg agagggcgca
     2341 cccgtaaggg tctttgacaa ggccaagggt gactaccgaa atgcagaatt cagggacata
     2401 gtaatactcc ttagaactac tgccaattgg gccgatacct ttcaggagga attatccata
     2461 caggggatac ctgtatactc cgacagcgga acgggatatt ttaagaccat tgaggtacag
     2521 accatgctgt cgctgcttca gataatcgac aaccccatgc aggacatccc gttattggcg
     2581 gttttaagat cccccattga aacatggagc ccggaagagc ttatcgacat caggctcacg
     2641 gaccgggaat ccacctttta cgaggccctt gtgaagaaat cccgggaagg ggatagtagg
     2701 ctggcccaaa aggcccggac ctttattgaa aaattaaacg gttggaggga tatatcccaa
     2761 tacctgtcca ccgacgagct tatctggcat ctgtacggcg agacgggata ctacagctgc
     2821 gtcggagcgc ttccgggggg agtgcaaagg caggcgaacc ttcggattct gtttgaaagg
     2881 gcccggcaat atgaagagac cagctacaag gggctgttta actttgttca ttttattgac
     2941 aggcttaaaa ccagtcaggg ggacatgggc agcgccaaga tcctggggga aaacgaaaat
     3001 gtggtcagga taatgagcat tcacaagagc aagggcctgg aatttcccat agtgtttgtg
     3061 gccggatgcg gtaagcagtt taatctaacc gacaccaccc gaaacatact atttcaccac
     3121 gacttgggat tggggccgga ttatgtagac tacgatagga ggatcatcta ttccaccctg
     3181 gccaaggaaa gcctcaaata caggattaca ctagagacct tgtcggagga gatgcggata
     3241 ttatatgtcg ctcttaccag ggcaagggaa aggctcatca taaccggcgc ggttaaggat
     3301 cttaaaagag agattagcaa atgggcagat gccttgtcta ttgaggacct taaacttccc
     3361 ggatacgaaa ccatgagggc aaggcggttt ttagactgga tagggcctgc aatcatcaga
     3421 catggtgatg gagcaccttt aaggcaaatt cttga
//
LOCUS       DASRNX010000204        11338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_725149,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000204 DASRNX010000000
VERSION     DASRNX010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11338
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725149"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /gene="miaB"
                     /locus_tag="VFD89_08050"
     CDS             <1..1344
                     /gene="miaB"
                     /locus_tag="VFD89_08050"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZJ58172.1"
                     /translation="DINKLIKNNGFKYHIITYGCQMNVHDSEKLAGMLNSMGYTETSG
                     QEEADLILFNTCCVRENAENRVYGNVGSLYPYKQQNPNLIIGICGCMMQQSEVAADIT
                     RKFPFVDLIFGTHNLHRFPELLFNAISSKETIIEILDEEGRIVEDVPIRRNTGVSAWT
                     TIMYGCNNFCSYCIVPYVRGRERSRQSSDILDEISRLGQEGYKEVTLLGQNVNSYGKD
                     LDNPYHFPNLLMDIDKVQGIERIRFMTSHPKDLSGDLIRAMAESNKVCEHLHLPIQSG
                     SDRILRAMNRRYNRETYLGLIERVRQQLPHISITTDIIVGFPGETDEDFADTLDIMER
                     VRYDSAYTFIYSPRTGTPASKMPGQIDSRIKKERLRRLMDVQGRISREINDATLGKTY
                     EVLVEGLSKNRNDMYTGRTRENKLVNFAAPEDVVGKIVKVRITQPKTWTLEGIMV"
     gene            1380..3998
                     /gene="mutS"
                     /locus_tag="VFD89_08055"
     CDS             1380..3998
                     /gene="mutS"
                     /locus_tag="VFD89_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZJ58173.1"
                     /translation="MSKLTPMMQQYMKIKEQNKDALLFFRLGDFYEMFFHDAEVASRE
                     LDIALTARDCGLPERAPMCGVPYHAAEGYIARLIERGYKVAICEQLEDPAEAKGIVKR
                     DVVRIITPGTLLEASMLDERNNNYILSIYRGKSGFGLSWADISTGEFYLTQMPHQKEL
                     GNMIDLISGIVPSEVIVQEGLESDALPLEIIQTRYPFIVTPLSQWAFEYNTAYQKLIV
                     HFDVHSLNGFGCEHMPKGISAAGALLEYLSQTQKNALKQITSLRPHHTGSAMVLDSAT
                     RINLELTETIRSKTKKGSLLWLLDKTITAMGGRLIRQWIQQPLTDSHLINQRLDAVGE
                     LCADPVSMEDLRHHLRMIYDLERLMGRVSYGSANARDLISLKQSVAVLPMIEDIVKGF
                     QSDIFKGFHEGFDLLQDIHILIEDSIDNNPPITIREGSLIKDGYNGQLDLYREAMTKG
                     KDWLADLEKDERKRTGINTLRVGFNKVFGYYLEVTKSYFDLVPDNYIRKQTLVNSERY
                     ITPELKEVEDSILGAEEKSVILEHKLFVDIREKIAAQVKRVQETARIISILDGLWSLA
                     RVSLENRYIRPQVDDGDVIDIQDGRHPVVEKTLPNALFVPNHTYLDSSENQIAIITGP
                     NMAGKSTYMRQVAVIVLMAQIGSFVPASKSRIGVVDRIFTRVGASDDLSAGQSTFMVE
                     MNEMANILHNATPKSLLILDEIGRGTSTYDGLSIAWAIVEYICNHKRLGSKTLFATHY
                     HELTELEGKLRGVKNFCITVKEQGNDIIFLRKVVRGGAEKSLGIQVARLAGLPLPVIN
                     RASKILNRLEKLDVNRAEDKSPLKEDTPQLSFFDQRPSEIEEEVRGIDILNITPIEAL
                     NILNKLVEMTRGSEDV"
     gene            4000..5808
                     /gene="mutL"
                     /locus_tag="VFD89_08060"
     CDS             4000..5808
                     /gene="mutL"
                     /locus_tag="VFD89_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZJ58174.1"
                     /translation="MDRILILDDMTINQIAAGEVIERPASIIKELVENSIDAEASAVT
                     IEIRDGGTSFIRITDNGRGMNPTDARVSFQRHATSKIGSSTDLYNVITLGFRGEALAS
                     IAAVTQTEMITREQHSISGTQIINHGGRIIAEKEIGCPEGTTIIVQNLFYNTPARFKF
                     LKSPRTETAYISDIVNRFILARPDISFKYINNGKMIYYSPGDNDLKHAALSVYGRDAV
                     AQVLTVEPIDTEIGIKLWGLLGEPPLARKTRSHQSFFVNKRYVKSKLLEEGVEAAYKP
                     YLTINQFPWIVLHVEIDPARIDVNVHPAKTEIRFRDEEKVDNIIQRHIGAILEENPYI
                     PIIKEGSFISFDTTDESTDKSQQLEVFPDVSLPWGGDKADKDEHTTNVPPSKLLETGN
                     EDYDIDWDIEKDNVFANASMPFKVVGKIFATYILVEGSRQCFLIDQHAAHERLVFERY
                     KEMLAKQDVATQQLLPPAVIEVTHSEYIIINESMDILHSLGFEIEDFGGRSFAIRGVP
                     VILGSSNLRELFGELLYGIKELGQGANYELRIEDVIRMSCKKAVKAGDSLSNIEILAL
                     LKDLMEKKMPMTCPHGRPIMISLTQMELEKMFRRIQ"
     gene            5834..6787
                     /gene="miaA"
                     /locus_tag="VFD89_08065"
     CDS             5834..6787
                     /gene="miaA"
                     /locus_tag="VFD89_08065"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZJ58175.1"
                     /translation="MGKIPLVIILGPTAVGKTGISIEIAEKLDGEIISADSMQVYRYM
                     DIGTAKPSEEERRGIPHYLVDVVYPHEEFNVSLYKQLADERIKKIHSDGKLPLVAGGT
                     GLYINSLLYSMDFTEVKGNSEYRNHLTKLMEAKGKDWLHNELLLADPESANRLHPNDT
                     RRIIRALEVHHITGRPISSHNRPDCLTTCEYSATLIGLTMERNMLYERIDSRVEEMLL
                     NGLLDEVRQLIEMGYGIHLNSMQGLGYKEIAQYLIGRRTLAESIHILKRDTRRFAKRQ
                     FTWFRRLPNVNWVDVGRFSSRNELKGHLVALIEKDLTNKGV"
     gene            6869..7117
                     /gene="hfq"
                     /locus_tag="VFD89_08070"
     CDS             6869..7117
                     /gene="hfq"
                     /locus_tag="VFD89_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HZJ58176.1"
                     /translation="MTNKGVIVFQDIFLNLVRKDHIPVTIHLVNGFQIRGFIKGFDNY
                     VIIVESEGKQMMLYKQAISTITPSRPVPLTGENNEGNQ"
     gene            7504..8766
                     /locus_tag="VFD89_08075"
     CDS             7504..8766
                     /locus_tag="VFD89_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014643365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HZJ58177.1"
                     /translation="MKSEGIIQRSQKAVTPRFEGVDELAEYNQLRVLKGMQECRISDT
                     HFAPTTGYGYGDEGRTRLEELYALVFGSQDALVRPQWGSGTHVISDALYAVLRPGDVL
                     VSITGKPYDTLEKTIGLDQDEPPQGSLMDWGISYNQIELDATGEIDTPKVLNMLDNYD
                     SIKAILIQRSRGYQWRSSISVYDMEQAISKIKEKSPNVFIIVDNCYGEFTQGIEPSHI
                     GADLTVGSLIKNPGGGLTPTGAYAVGTKEAIELLSYRLTSPAIGREVGSYAASYLPFF
                     QGLFMAPHVVGQALKGAILASHLFKALGYEVLPHWEDERGDIIQCIKFGSDEELIAFC
                     QAIQQASPVDGHVTPVPWEMPGYTHEVIMAAGTFIQGASIELSADAPITPPYIAYLQG
                     GLTYAHVKLALSSVISRLYEGGFVSIKD"
     gene            complement(9027..9641)
                     /gene="lexA"
                     /locus_tag="VFD89_08080"
     CDS             complement(9027..9641)
                     /gene="lexA"
                     /locus_tag="VFD89_08080"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZJ58178.1"
                     /translation="MSNYINDNQEMVLNFIKQMVTEKGYPPSVREICQGVGIKSTSTV
                     HGHLEKLEKAGLIRKDPTKPRAIEILDETTHLSKKELINVPIVGKVTAGQPILAVENI
                     EDTFPLPAEYLHNGDVFMLTVVGESMIEAGIMDGDYVIVRQQNYAENGDAVVALIEDE
                     ATVKTFYKENDYIRLQPENTAMEPIIVKDILVLGKVIGVIRLFP"
     gene            9904..10221
                     /locus_tag="VFD89_08085"
     CDS             9904..10221
                     /locus_tag="VFD89_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZJ58179.1"
                     /translation="MSPLHRNQKKKIKVTNKARLSIFISILLVVLYPAMMFAIDKPHN
                     KIAPSFINVYIEPGDTLWDIAKANLPPKTDIRDFIHEIKSINELDSALIVEGDSLLVP
                     FNP"
     gene            complement(10233..11294)
                     /locus_tag="VFD89_08090"
     CDS             complement(10233..11294)
                     /locus_tag="VFD89_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZJ58180.1"
                     /translation="MSNHDSYYQKELQYNTKRLRNLIRDLPSLCGEYFRGIEHTTSIL
                     TRINYAYDLRIFFDFLITEIDAFTDLSPRELSVEHLDEIQILHIEKYLEYTTYYSNPN
                     NLEQERQNGEMGKARKLASLRSLLSYFYKKEKIQRNVAQLVDLPKLNEKPIIRLDVDE
                     VARLLDIVESGKKLTPTQQRYHQYTGSRDLAILTLLLGTGIRISECVGLNILDFDFRN
                     NGFSVTRKGGDQVILYYGDEVKTAILDYIEERKKIETLPGHEDALFLSIQRRRISHRA
                     LQNLVKKYAKIVAPLKRITPHKLRSTYGTTLYQETGDIYLVADVLGHRDVNTTRKHYA
                     AIGDEQRRIAARVVKLRGD"
BASE COUNT         3398 a         2207 c         2588 g         3145 t
ORIGIN      
        1 gatataaata aattgattaa aaataatggg ttcaaatatc acatcataac ctacgggtgt
       61 cagatgaacg tccatgattc tgaaaaattg gccggtatgc tgaattccat gggatatact
      121 gaaacttctg ggcaggagga ggcagatctt attctattta atacctgctg cgtgagagaa
      181 aatgccgaga acagggtcta cggtaatgtt ggttccttgt acccctataa gcaacagaac
      241 cctaacctaa taattgggat atgtgggtgt atgatgcaac agagtgaggt agccgcagat
      301 ataacccgaa agtttccatt cgtggattta atcttcggta cccataactt acatcgtttt
      361 cccgagctat tgtttaacgc aattagttct aaagaaacta ttattgagat actagatgaa
      421 gaaggacgga tagtagagga tgtacccatt aggaggaata ccggggtctc ggcatggact
      481 actataatgt atggctgtaa taatttctgc agttattgta ttgttcccta tgtccgaggc
      541 agagaacgaa gcaggcaatc ttctgatata ttggacgaga ttagccgatt gggtcaagaa
      601 ggatataagg aagttaccct tctgggtcaa aacgttaatt catacggaaa ggatctagat
      661 aacccatacc attttccaaa cttattaatg gatatagata aggttcaagg tatagaacgg
      721 attaggttta tgacatctca tccaaaggat ttgtcagggg atctcattcg cgccatggcg
      781 gagtccaaca aggtatgcga acatttacat ctgcccatac aatccggtag cgatagaatt
      841 ctcagagcta tgaatcgtcg ctataatagg gagacgtatc ttggactaat tgagcgggtt
      901 cgccagcagc ttccccacat atccatcact acggatataa ttgtcgggtt ccccggtgaa
      961 accgatgaag attttgctga taccctggat ataatggaga gggtcaggta tgattctgcg
     1021 tatacattta tatattcgcc aagaaccgga actccagcgt caaaaatgcc gggacagata
     1081 gacagtagaa taaaaaagga acgcctccga agattgatgg atgtccaggg tagaataagt
     1141 agggaaatta atgatgccac ccttggtaaa acatacgagg tcttagtgga gggattgagt
     1201 aaaaatagga atgacatgta taccggcaga acccgggaaa acaaattggt aaacttcgcc
     1261 gccccggagg atgtagtggg caagatcgta aaagtaagaa ttacccaacc caagacttgg
     1321 accctggagg gaataatggt ataataggct acgaacagtt cacttatggg agggttttta
     1381 tgtccaagtt aaccccgatg atgcaacagt atatgaaaat caaagagcaa aacaaggatg
     1441 ctcttctttt ttttagatta ggtgatttct atgaaatgtt tttccacgat gcagaggtag
     1501 cgtcaaggga attggatatc gccctgactg caagggattg cggtctgcct gagagggccc
     1561 ctatgtgtgg ggttccctac catgccgcag agggttatat agccaggcta attgaaaggg
     1621 gatacaaagt agccatttgc gagcaattgg aggatccggc tgaggctaag ggcatcgtta
     1681 agcgagacgt agttcgcatt attactccag ggaccctatt agaagcatct atgctggatg
     1741 aaagaaataa taattacatc ctgtcaatct acagggggaa aagtggtttc gggctgtctt
     1801 gggcggatat ttctaccggc gaattttatc taacccagat gccccatcaa aaggagttag
     1861 gcaatatgat cgatttgata tcgggtatag tgcccagtga agtgatcgtt caagagggtc
     1921 tagagtcaga cgcgctgccc ctggagatca tccaaacccg atatcccttc attgtaacgc
     1981 ccctgagcca atgggcattt gaatataaca cggcttacca gaaactcatt gttcattttg
     2041 atgtccattc cctaaatggg tttggatgtg agcatatgcc aaaggggatc agcgctgccg
     2101 gtgcattact ggaatattta agccaaaccc agaaaaacgc cttgaagcaa atcacttccc
     2161 taagacccca tcatacgggt tctgcgatgg ttttagattc ggctacccga attaatctgg
     2221 agcttacgga gacgatacga tctaagacta aaaagggatc cctgctttgg ttgttggaca
     2281 agaccattac cgccatgggc ggaaggctga taagacaatg gattcagcaa ccccttaccg
     2341 attcccatct aattaatcaa aggttggatg ccgttggaga actatgtgcc gatcctgtgt
     2401 ctatggaaga tctcaggcat catcttagga tgatctacga tctggaaagg cttatgggta
     2461 gggtatccta tggcagcgct aatgctaggg acctaatatc attgaagcaa tctgtagctg
     2521 tactcccgat gatagaagat atagttaagg ggtttcaatc tgatatattt aagggctttc
     2581 atgaaggctt tgatctcctt caggatatcc atattcttat agaagattcc atagataata
     2641 atccccccat aaccatcagg gagggatccc ttattaagga tggatacaat gggcaactgg
     2701 acctatatcg tgaggccatg actaaaggca aagattggct ggccgaccta gaaaaggacg
     2761 agagaaagcg tacggggata aatactctga gggtcggttt taataaggtt tttgggtact
     2821 acctggaggt taccaaatca tattttgatc tggtccctga caactatatt aggaagcaaa
     2881 ccttagttaa ttctgaaagg tatataaccc ccgaattgaa agaagtggag gattccattc
     2941 taggggcgga ggaaaagagt gttattcttg agcataaact ctttgtggat atacgggaaa
     3001 aaattgcagc tcaagtaaaa agggtacaag agactgctcg gattatatca atcctggacg
     3061 gcttatggtc cttggcccgg gtaagccttg aaaatcggta cattaggcct caagttgatg
     3121 atggggatgt tatagatatc caagacggaa ggcatcctgt agtagaaaaa accctgccta
     3181 atgctctgtt tgtgcctaac catacctatc tcgacagctc tgaaaatcaa attgccatta
     3241 taacaggtcc caatatggcc ggtaagagca catatatgag acaggtcgca gtcattgtac
     3301 tcatggccca aattgggagt tttgtacctg ccagcaaatc tcgcattggg gtggtggaca
     3361 gaatttttac aagggtaggg gcctcagacg atctctctgc aggtcagagc acgttcatgg
     3421 tcgaaatgaa tgagatggca aatatattac ataatgctac ccctaaaagc ttgttgatac
     3481 ttgatgagat cggaaggggc accagtacct acgacggtct cagcattgct tgggcaatag
     3541 ttgaatatat atgcaatcac aagagattgg ggtcgaagac tctctttgcc acccattatc
     3601 atgagcttac agagcttgaa ggtaagctcc ggggggttaa gaacttttgt attacagtaa
     3661 aggaacaagg taatgatata atatttttaa gaaaggttgt acggggggga gctgaaaaga
     3721 gcctgggtat tcaagtagct agattggcgg gcctcccact gccagttatt aaccgggcct
     3781 ctaagatatt gaacagatta gaaaaattag atgtcaacag agctgaagat aagagcccct
     3841 taaaggaaga taccccccaa ctgtccttct ttgatcagag accctccgaa atcgaggagg
     3901 aggtaagggg tattgatatt ctcaatataa ccccgattga ggcgttgaat atactgaata
     3961 aattagtgga aatgactagg ggaagcgagg atgtgtagaa tggatcggat tcttatttta
     4021 gatgatatga caataaacca aattgcagcc ggggaggtta ttgagcggcc ggcctccata
     4081 ataaaggaat tggtggaaaa ctccattgac gctgaggctt ctgccgtaac catagagatt
     4141 cgtgatgggg gtacgtcctt tattagaatt accgataatg gtcggggcat gaacccaacg
     4201 gatgctaggg tgtcctttca aaggcatgca acgagtaaga ttgggtcctc cactgaccta
     4261 tataacgtta tcactttggg ttttaggggt gaggctctgg caagtattgc ggcagtaacc
     4321 caaacggaga tgataacacg ggagcagcat agtatatcag gaacgcagat aataaatcac
     4381 ggtgggcgta ttatagcaga aaaagaaata gggtgcccgg aaggtacgac cataattgta
     4441 cagaatctat tttataatac tccggccaga tttaagtttc ttaagagccc cagaacggaa
     4501 acggcatata ttagcgatat agtaaataga ttcatcctcg cacggccgga tatatccttt
     4561 aagtatataa ataatgggaa aatgatctat tattcacccg gcgataacga tcttaagcat
     4621 gctgctttga gtgtctatgg cagggatgcg gttgctcaag tacttacggt agagcccata
     4681 gacaccgaaa taggcataaa attatggggc cttctggggg aaccgcctct tgccagaaaa
     4741 acgcgcagcc atcaatcctt ctttgtcaat aaacgctatg ttaaaagcaa actgttggaa
     4801 gagggcgtag aggccgctta taagccctat cttaccataa accaatttcc gtggattgtt
     4861 cttcatgttg aaattgatcc cgctagaatt gatgtgaacg ttcatcccgc aaagaccgaa
     4921 attcggttta gggatgagga aaaggtggat aacataatac aacggcatat tggagctata
     4981 cttgaggaga atccatatat acctataata aaagaaggct cgtttatatc ctttgatacg
     5041 accgatgaaa gcacagacaa aagccaacag ttagaggtat tcccagatgt ctcgctgcct
     5101 tggggtgggg ataaagccga taaagatgaa cacactacaa atgttccccc atctaaactg
     5161 ttagagaccg ggaacgaaga ttacgatatc gattgggata tagaaaagga taatgtattt
     5221 gctaatgcat ctatgccttt taaagtggta ggcaaaattt ttgccaccta tatactcgtg
     5281 gagggcagca gacaatgctt cttaatcgat cagcatgcgg cgcatgagcg cttagttttt
     5341 gaaaggtata aggaaatgct agcaaagcag gacgtggcta cccaacaatt gcttcccccc
     5401 gcagttatcg aagtaaccca ttccgaatat ataataatca atgagtccat ggatattctt
     5461 cattccttgg gttttgaaat agaggatttt ggtggcagat cctttgctat tcgcggtgta
     5521 cccgttattt tgggctccag caatcttaga gaattgtttg gggaattgct ctatggcata
     5581 aaggaactcg gacagggagc caactatgag ctgagaatag aggatgttat tcgaatgtcc
     5641 tgtaagaagg cggtaaaagc cggggattcg ctttccaata tagagatttt agccctattg
     5701 aaggatttaa tggagaaaaa aatgcccatg acctgcccgc acggaaggcc tataatgatt
     5761 tcactaacac agatggaatt agaaaaaatg ttcaggcgga tacaatagat caatcattag
     5821 aaaagaggtt tttatgggta aaataccatt agttataata ttaggaccca ctgcagtagg
     5881 taagaccggg atctccattg aaatagcgga aaaactcgat ggggagatta tatctgccga
     5941 ttcgatgcag gtctacagat acatggatat tggcacggct aaaccctcgg aggaagaaag
     6001 gcggggcatt ccccactatt tggtagacgt ggtttatcca cacgaagaat tcaacgtttc
     6061 cttgtataag cagctggctg atgaaaggat caagaaaatt catagcgatg gaaaacttcc
     6121 cttggttgcc ggcgggacgg gactatatat aaactccctt ctctattcca tggactttac
     6181 tgaagtaaaa ggaaatagtg aatatcgtaa ccacctaacg aaactaatgg aagcgaaggg
     6241 taaggactgg ttgcataacg agctgctttt agcagacccc gaatctgcta atcgcctcca
     6301 tcccaatgat acgcgtcgta taatcagagc cttggaagtt catcatatta ccggccgacc
     6361 aatatctagt cacaataggc ccgattgttt aactacatgc gaatattccg ctactctgat
     6421 aggtctgacg atggaacgta acatgctata tgaacgtatt gatagccgcg ttgaagaaat
     6481 gcttctaaat ggtctattgg acgaagtgag acagcttata gaaatgggat atgggatcca
     6541 cctcaattca atgcaggggc taggttacaa ggaaatagca cagtatctga ttggaagacg
     6601 tactttagca gagtctatcc atattttaaa gcgcgatacc cgtagattcg ccaagcggca
     6661 gtttacctgg tttagacgat tgcccaatgt aaattgggtg gatgtaggaa ggttttccag
     6721 tagaaacgaa ctaaaagggc atttagtcgc tctcattgaa aaagatctca caaataaggg
     6781 cgtgtaagaa aatccacttt tattaccatg gtaattggta tataatagaa ttgtacgcaa
     6841 ataataagat tagaagggtg gtactaaaat gactaataaa ggagtaattg tttttcaaga
     6901 tatattttta aatttggtgc gaaaggatca tataccggta actattcatc tggttaatgg
     6961 gtttcagatc cgcggtttta ttaagggatt tgataactat gtaataatag tggagagcga
     7021 gggaaaacaa atgatgctgt ataagcaggc tatttccaca ataaccccct ccagaccagt
     7081 cccattaacc ggtgaaaaca atgaaggaaa tcagtagtat ttctacatat aaatcttata
     7141 gtattgtaca gaagggacca tagggttcct tttggttata acatcggaag caattgcatt
     7201 ggcaattgca acacacaggt ttcaacgagg cggaggattt gtacccaccg actgttttac
     7261 taatcggaac ttgccgctta tagaagcgag agacatcttg ccgactcgtt atatgcacta
     7321 taaaaactat ttgttctaga gatcttatat ggaaaagcaa ctataatgaa ctcgctataa
     7381 tgattggggt ttcacgaaaa catattgttt taatagatat tttatgacga atacagtaag
     7441 gagagcatgg tgggaactaa taacttattc tatacaagat tgaagcggga atttggtatt
     7501 aacatgaaat cagaaggtat catacagaga tcgcaaaagg ccgtaacgcc tagattcgaa
     7561 ggcgttgatg agctggcaga atataatcag cttagggtcc ttaagggcat gcaagagtgt
     7621 aggatcagcg acacacactt tgcccccacg acaggttacg gctacggcga tgaaggtcgt
     7681 acccgtttgg aagaattata tgcgttggtg tttggtagcc aggatgcgtt ggtcagaccc
     7741 caatggggtt cgggcaccca tgtaataagc gatgccttgt atgctgttct gcggcccgga
     7801 gatgtcttag tgtctatcac cggaaaaccc tatgatacct tagaaaaaac cataggtctg
     7861 gaccaagatg agccacccca gggttcctta atggattggg gtatttcata taatcagata
     7921 gagttggatg ccacagggga aatagacact ccaaaggtac taaatatgct ggacaattac
     7981 gatagcatca aggccatttt aatacagagg tccaggggat accagtggcg atcttccata
     8041 tctgtttacg atatggaaca agccataagt aaaataaaag aaaagtctcc aaacgttttt
     8101 attatagtgg ataattgcta tggggaattt actcagggta tagaaccaag tcatattggc
     8161 gctgatttga ctgtgggttc attgattaaa aatcctggtg gcggacttac acccacaggt
     8221 gcttatgcgg tgggaacaaa agaggctatt gaactactat cctatcgttt aacttcgcct
     8281 gcaattggcc gggaggtggg ctcttatgct gcatcttatc ttcctttttt ccagggtctt
     8341 tttatggctc ctcatgtagt aggtcaagca ttaaaaggag caattttagc ttcacacctt
     8401 ttcaaagcct tgggatatga ggtgttaccc cattgggagg acgaaagggg agacataatc
     8461 cagtgcatca aatttggctc agacgaggag ttaatagcct tttgccaagc cattcaacag
     8521 gcttctccag ttgatgggca tgttaccccc gtaccctggg aaatgccggg atatacacat
     8581 gaagtcatta tggcggcagg aacctttatt caaggggcgt ctattgaatt aagcgctgat
     8641 gcgcctatta caccacctta tattgcttat ctgcaaggcg gtctcaccta tgcacatgta
     8701 aagctggcgt tatcatccgt tatatccagg ctttatgaag ggggatttgt atccataaag
     8761 gattaaattt tgtattgaaa atccttccca gagctcattg ccctttggaa aggattctat
     8821 atagttgttg tattataacg agtatgcaag atgtctcccg cttctataag cggcgagttc
     8881 ccattagtaa acaggcggtg agtataaatc tcccgcctcg ttgaaacgtg tgtgttgcaa
     8941 ttgccaatgc aattgcttcc gatgttttaa ttgtcacatg aactgaatcg acaaggtagc
     9001 gctatatata agatttgatt attagattac gggaatagac gaatcacgcc aataaccttc
     9061 cctagaacaa gaatatcctt tactattatc ggttccatag cggtattttc aggctgtaat
     9121 ctaatataat cattttcttt atagaaggtc ttgactgtag cctcgtcttc tatcaaggca
     9181 accacagcat ctccgttttc cgcatagttt tgttgtctaa caatgacata atctccatcc
     9241 ataatcccgg cctctatcat actttcacct actacagtta gcataaaaac atctccgttg
     9301 tgcagatact ccgccggcag gggaaaggta tcttcgatat tttccaccgc cagaataggt
     9361 tgaccggctg taactttccc cacgataggg acattaatca attctttttt tgaaaggtga
     9421 gtcgtttcat caagaatttc tatagcccgg ggtttagtag gatcttttcg tatcaagcct
     9481 gccttttcca atttttctaa atggccatgc acagtggagg tggatttaat gcccactccc
     9541 tgacatattt cgcggacgga ggggggatac cccttttctg tgaccatctg ctttataaaa
     9601 ttcaggacca tctcctgatt gtcatttata taattagaca ttcatctgca cctctcttta
     9661 taggttacag acattataac atagacaaga catcatttca aacagatgtt cgcaccgtcg
     9721 gttatatctt tacaatttta tacgttttga catttgatag ggcaggaagg tgcagcgaat
     9781 atccgtcata atagacaaat atcataaatt ggacataggt ttagcaaata atcccttgac
     9841 acagaacaca tgttcatcta taatatactc ataaactgaa cgtatgttct aagggggaaa
     9901 atcatgagcc cactacatag gaatcaaaaa aagaaaatca aggtgaccaa taaagcccga
     9961 ttaagtatat ttatatccat tctcctggtt gttctatatc ctgcaatgat gttcgctatt
    10021 gacaagcccc ataataaaat agctccgtcc tttatcaatg tttatatcga acccggggat
    10081 accttgtggg atatagcaaa ggctaatttg ccgcctaaaa ccgatatcag ggattttatc
    10141 cacgaaatta aatcgattaa tgaattggat tcggctctta ttgtggaagg ggattcgcta
    10201 ttggtacctt ttaatccata aaaatagggg gattaatccc cccttaactt aactaccctg
    10261 gcagcgatcc ttctttgctc atcaccgatg gcagcataat gctttcttgt ggtatttaca
    10321 tccctatgtc ctaaaacatc cgccaccaga tagatatcac cggtctcttg gtatagggta
    10381 gtaccatagg tgctccgcaa tttatgtgga gtaatcctct ttaaaggagc cacaatcttg
    10441 gcatacttct ttactaagtt ttgcaatgct ctatggctga ttcgtcttct ctgtatagaa
    10501 aggaataagg cgtcttcgtg tcccggtagg gtttcaattt tttttctctc ctcaatataa
    10561 tccagtattg cggtctttac ttcatcccca tagtataata tcacctgatc tccgcctttt
    10621 ctagtcacac taaagccgtt atttctgaaa tcgaaatcta atatattcaa tccaacacat
    10681 tcgctgatac gtattcctgt gcctaaaaga agggttagga tagccaaatc cctggatccg
    10741 gtatattgat ggtatctctg ctgggtgggg gttagcttct ttccggattc tacgatatcc
    10801 aatagcctcg caacttcgtc aacatccaac cttattatgg gcttctcatt aagtttgggg
    10861 agatctacca gctgtgcaac atttctttgg attttctcct ttttataaaa atacgacaat
    10921 agggagcgga gggaggccaa tttccttgct ttgcccattt caccattttg tcgttcctgc
    10981 tccaaattat tgggattact atagtatgta gtgtattcca aatatttttc tatgtgtagg
    11041 atctgtatct catccaagtg ctccactgat agctcccggg gggagaggtc ggtaaaagca
    11101 tcgatttccg tgatcaaaaa atcaaagaat attctaagat cgtatgcata attaatcctg
    11161 gtaagaatgg aagtagtatg ttcgatacca cgaaaatact caccgcaaag gctaggtaag
    11221 tctcttatta ggttccgaag ccttttagta ttatattgga gttctttttg atagtaagag
    11281 tcatgattgg acacataaac acttcctttt ttggtatata ggcattcctg aatagaat
//
LOCUS       DASRNX010000205         4263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_306399,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000205 DASRNX010000000
VERSION     DASRNX010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306399"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..266
                     /gene="rpsP"
                     /locus_tag="VFD89_08095"
     CDS             18..266
                     /gene="rpsP"
                     /locus_tag="VFD89_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZJ58181.1"
                     /translation="MAVKIRLKRIGAKKAPYYRVVVADSRSPRDGRFIEEIGYYHPLA
                     NPSVVKIDAEKAKQWLQNGAQPTDTVRALLKKNGIIEA"
     gene            329..556
                     /locus_tag="VFD89_08100"
     CDS             329..556
                     /locus_tag="VFD89_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZJ58182.1"
                     /translation="MGELVKFIAKSLVDNPDEVDVREVEGEQTVIIELRVAPDDMGKV
                     IGKQGRIAKAIRTVVKAAATKEKKRVVVEII"
     gene            576..1097
                     /gene="rimM"
                     /locus_tag="VFD89_08105"
     CDS             576..1097
                     /gene="rimM"
                     /locus_tag="VFD89_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZJ58183.1"
                     /translation="MLKHLTVGYVLRPRGIKGEVKVEPLTDSVDEFHKWDTLYIKDGN
                     KYYPLKINAKRNTGDNLFLHFEGYDDMNMAEKLRGKYLWIPRDEMEELPEDTFYITDI
                     LGCTVYTQEGAVLGQVDRIIHTGSNDVYLLKGSAGEILIPGLKKVILDIDITAKKMVV
                     AAQELEGLLPDAD"
     gene            1087..1821
                     /gene="trmD"
                     /locus_tag="VFD89_08110"
     CDS             1087..1821
                     /gene="trmD"
                     /locus_tag="VFD89_08110"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZJ58184.1"
                     /translation="MRIDILTLFPEMFEGIMDTSILGRARDKGILTINPVNIREFAKN
                     KHRQADDYPYGGGPGMVMSPQPLFDALYHVLEDSPDARVVYFTPQGKTLKQKMAEGYA
                     KRSHLILLCGHYEGIDQRVVDRFVDDEISIGDYVLTGGELPAMVFIDCISRLIPGVLG
                     SALSAQDESFSDGLLEYPHYTRPQIYEGLGVPEVLLSGHHAKIDRWRRDESLRNTLNK
                     RPDLLENADLTPEDIETVKRLKDDGK"
     gene            1942..2286
                     /gene="rplS"
                     /locus_tag="VFD89_08115"
     CDS             1942..2286
                     /gene="rplS"
                     /locus_tag="VFD89_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZJ58185.1"
                     /translation="MEIIRTIEKEQMKKDLPKFNIGDTVRVYVKVVEGTRERLQAFEG
                     IVIQKRHGGLRETFTVRRLSYGVGVERVFPVHSPKIDRIDVIRRGKVRRAKLYYLRDR
                     IGRAARVKERNL"
     gene            2486..3001
                     /gene="lepB"
                     /locus_tag="VFD89_08120"
     CDS             2486..3001
                     /gene="lepB"
                     /locus_tag="VFD89_08120"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZJ58186.1"
                     /translation="MKDVKNDIREWVQAILIAVVVSAVVRLFFFETTLVYGNSMESTL
                     KNKDRVIINKIVYHLYTPSRGDIVVFKNPDNNKENYVKRIIGIAGDTIEIGDQEVYIN
                     DELLDEPYLTEVTKSDFPKLLVPQDTIFVMGDNRNHSQDSRFIGPIPMENIIGKAQLR
                     IWPIADFTFFR"
     gene            3020..3871
                     /gene="ylqF"
                     /locus_tag="VFD89_08125"
     CDS             3020..3871
                     /gene="ylqF"
                     /locus_tag="VFD89_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HZJ58187.1"
                     /translation="MNVNWYPGHMVKAKRLISEDLRLIDIVLEIRDARVPTSSANPDF
                     EDLFLNKSRIVFLNKSDLADPSHTGDWIKWFSEKGIKAVPVNSLDPKDTGRARKAILS
                     AAEAFHKEILEKRGIKKTIRGMAVGIPNVGKSAFINGIAGGKKAKTGNKPGVTRIKQW
                     IRINPYLELLDTPGLLWPRLDQRITALNLAYTRTIKEEILDIEEIAHNFLETAKLKFP
                     KALVSRYGELDMGLRGYEILSEICIQKAWIQAGGIADLMRGASHVLDDFQQGRLGRIT
                     LEMPAEI"
     gene            3881..>4263
                     /locus_tag="VFD89_08130"
     CDS             3881..>4263
                     /locus_tag="VFD89_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZJ58188.1"
                     /translation="MNNLSIDSLSIHKIKELVRSGSLEDLRVLLELIKLDDRKGVRSL
                     NRTILKRFKDEEDRFQRRSMLLKEERRLWAMGYKNIGGLDEAGRGPLAGPVVAACVVF
                     DPDTYIDGVNDSKKLSPSKREKLFQ"
BASE COUNT         1345 a          730 c         1121 g         1067 t
ORIGIN      
        1 ggaaaagagg tgatataatg gcagttaaga ttagacttaa aaggattggt gcaaaaaaag
       61 ctccctacta tagggttgta gttgcagatt ccagatcacc gcgtgacggt cgctttattg
      121 aggaaattgg gtattatcac ccattagcca acccttcggt agttaagata gatgctgaaa
      181 aggcaaaaca atggctacaa aatggtgccc agcccacgga cacagtccga gcacttctaa
      241 agaagaatgg tatcattgaa gcctaaatgc cagaatggtc cgcttataga attaacgacg
      301 gaaaaataaa catacggggg tattttaaat gggagaatta gttaagttta ttgccaaatc
      361 cctagtagat aatccagatg aagttgatgt cagggaagtc gaaggggaac agacggttat
      421 catagaacta agggtagccc ccgacgatat gggcaaggta attggtaagc aaggacgaat
      481 agcgaaggct atacgcacag tggtgaaagc tgcggcaact aaggagaaga aaagggttgt
      541 tgtagagatt atttaagcat gggaagtatg aaaaattgtt aaagcatctt accgttggat
      601 atgttttaag gcctagggga atcaaggggg aagtcaaggt tgagcccctt acggatagcg
      661 tagatgagtt ccataaatgg gacaccctat acattaaaga tgggaacaaa tactatccac
      721 tgaagattaa tgccaaaaga aataccggag ataatttatt cttgcacttc gaaggctatg
      781 atgatatgaa tatggcagaa aaactcagag gaaaatacct gtggataccc agagacgaga
      841 tggaagaatt acctgaggac actttttata tcaccgatat ccttgggtgt actgtttaca
      901 cacaggaagg tgcggtgctg ggtcaagttg ataggatcat tcacaccggc agcaatgatg
      961 tctatctgct caagggttct gcgggagaga tcttaatacc cggtcttaaa aaggtaatat
     1021 tagatataga cattacagct aaaaaaatgg tagtagcagc gcaggaattg gaggggttat
     1081 tgcctgatgc ggattgatat ccttaccctg tttccggaaa tgtttgaggg tataatggac
     1141 accagcattt tggggagagc cagggataaa ggtattctaa cgataaatcc cgtaaatata
     1201 agagagtttg ccaaaaacaa acatagacag gctgatgatt atccctatgg cggaggtccg
     1261 gggatggtta tgtccccaca acctcttttt gatgccctat atcatgtact ggaagattca
     1321 ccggatgcaa gggtagtata ttttacgccc caaggtaaaa ctctgaaaca gaagatggca
     1381 gagggatatg cgaagcgttc acacctaatt ctgctttgcg gacattatga gggtatagac
     1441 caaagggtgg tagaccgatt cgtagatgac gagatatcaa taggggatta tgtcctcact
     1501 ggcggggagt tgcctgccat ggtattcatc gattgtataa gccggctaat tcccggtgta
     1561 ttgggcagtg ccttatcagc ccaggatgaa tcctttagcg acggactgtt ggaatatccc
     1621 cactacactc gcccacagat ttacgagggg ctaggcgtac ccgaagtgct cttatcgggt
     1681 catcatgcta agatagaccg atggagaagg gatgaaagcc ttcgaaatac cctgaataag
     1741 agaccggatc tgttagagaa tgccgatttg acgcccgagg acattgagac agtgaaaagg
     1801 ttaaaagatg acgggaaatg acggaccaat tatttattgc catggaaatg gcggtatgct
     1861 ataattaatt ttatgattat ggacgttccg tgtgatagag tagattagga acgttttgga
     1921 tggaaggagg tagctgtaga tatggaaata attcgaacta ttgaaaaaga gcagatgaaa
     1981 aaggatctcc ccaagtttaa tataggagat accgttagag tttatgtaaa ggttgttgag
     2041 ggaacaaggg aaagattaca agcctttgaa ggcatagtta tacagaagag gcatggcggc
     2101 ctgcgcgaaa catttaccgt acgaagatta tcctacggtg taggcgttga aagggtcttc
     2161 ccggtacatt ctcccaagat tgaccgtatt gatgtaattc gaaggggtaa ggtacgcagg
     2221 gctaagttgt attacctaag ggatagaata ggaagagccg cgagggttaa agaaagaaat
     2281 ctgtgagaat gtgggactgg gggcagtccc atattttgta acgagtcggt aagatgtctc
     2341 ccgcttctat aagcggcgag ttccgattag taaaacaggc agtgggtata aatcccccgc
     2401 ctcgttgaaa cctgtgtgtt gcaattgcca atgcaattgc ttccgatgtt ttaatgtcct
     2461 tatttgattt gggagaaggc tgtatatgaa agatgtaaaa aacgatatta gggaatgggt
     2521 ccaggctata ttgatcgctg ttgttgtatc agcggtggtg cgcttatttt tctttgagac
     2581 cacattggtg tacggaaatt caatggaatc caccttgaaa aacaaggaca gggtaataat
     2641 aaacaagatt gtataccatc tatacacacc gtccaggggt gatattgtgg tgtttaaaaa
     2701 ccccgataac aataaggaga actatgtcaa gcgaattata ggtattgcag gtgatactat
     2761 cgagataggg gaccaagagg tatatataaa cgatgagctt ttggatgaac cctatttgac
     2821 agaggttact aaaagtgatt ttcccaaatt gttggttccc caagacacca tattcgttat
     2881 gggtgataac cgcaatcata gtcaggacag taggtttata ggtccgatac caatggaaaa
     2941 tatcattgga aaagcccaat tacggatctg gcccatagcc gattttactt tcttcaggtg
     3001 agggaaagga gtctttttat tgaatgttaa ctggtatccc ggacatatgg tcaaagcaaa
     3061 aaggcttata tccgaggatt taaggctaat agatatagta ttggaaataa gggatgcacg
     3121 ggttcccaca agcagcgcta atccggattt cgaagacctc tttttaaaca aatccagaat
     3181 agtctttctc aacaaaagtg acttagctga tccatcccat acaggggatt ggattaaatg
     3241 gtttagtgag aaagggataa aggccgttcc ggtcaattcc cttgacccaa aagataccgg
     3301 tagggcaagg aaggccattc tatccgctgc cgaggcattt cataaggaaa tactcgagaa
     3361 aaggggcata aaaaagacta tcaggggtat ggcggtcggg ataccaaacg taggaaaatc
     3421 agcttttata aacggcatcg ccggcggaaa gaaggctaag acagggaaca aaccgggggt
     3481 tacaagaatt aaacaatgga tcagaattaa tccgtatctg gagctgctgg ataccccggg
     3541 tctgttatgg ccgagacttg atcaaaggat tacagcctta aatttagcat atacccgtac
     3601 catcaaggaa gaaattttgg atatagagga gattgcccat aattttcttg agacagccaa
     3661 attgaaattt ccgaaagcgt tggtatccag atatggagaa ttggatatgg gcttgagggg
     3721 atatgagata ttatccgaga tatgtataca aaaagcctgg attcaagcag ggggaatagc
     3781 agaccttatg aggggcgcaa gccacgtgtt ggatgatttt cagcagggta ggttggggag
     3841 aattaccctg gaaatgccag ctgagatata gggacacatg atgaataatt tgagtatcga
     3901 cagtctgagc atccacaaga ttaaggagct ggtccgatcc ggttccttgg aggatttaag
     3961 ggttttgctg gagttaatta aactggatga taggaaaggc gtcagatcct tgaaccgaac
     4021 gatccttaag aggttcaagg acgaagagga caggtttcaa agacggagca tgttattgaa
     4081 agaggaacga aggttatggg ctatgggata taagaacatt gggggcttgg acgaggcggg
     4141 aaggggaccg ttggccgggc cggtggtagc agcctgtgta gtctttgatc cggatacata
     4201 tatagacggt gtaaatgatt caaagaagct ttccccctct aaaagggaaa aactcttcca
     4261 aat
//
LOCUS       DASRNX010000206         4040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_520273,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000206 DASRNX010000000
VERSION     DASRNX010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520273"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..632
                     /locus_tag="VFD89_08135"
     CDS             39..632
                     /locus_tag="VFD89_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HZJ58189.1"
                     /translation="MKNLKGTKTAENLMKAFAGESQARNRYFMYASVAGKEGYKQIEE
                     IFLETADNERIHANRFFKFLVEGFGDELPTTVEINADYPVALGNTLENLKAAAAGEHE
                     EWSDLYPAFAQVAKEEGFPEVATAFTMIAKAEVSHEARFNKLADNVKDGKVFKKDEPT
                     QWICRKCGYIHTGEKAPEVCPTCLHKQPHFQLFKETY"
     gene            complement(689..952)
                     /locus_tag="VFD89_08140"
     CDS             complement(689..952)
                     /locus_tag="VFD89_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58190.1"
                     /translation="MEIYQVAIIPLIIGVVELFKSLGLPNKFSALTASLLGILIGVFY
                     VYPRDVPRGIIVGLSFGLAASGLYSGTKNTVQGVKEMGIPRKK"
     gene            complement(1158..2288)
                     /locus_tag="VFD89_08145"
     CDS             complement(1158..2288)
                     /locus_tag="VFD89_08145"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZJ58191.1"
                     /translation="MKEIGIGIIGYGFMGKAHTYAYRTMPLYYEDLPFRIRLVGICNR
                     TLEKAQRAKEDLGFEFATDDPNELFAADNIDIINICTPNNLHKEHVIGALKAGKHVYC
                     DKPLSATYQETEEILNVLASSKAITQMAFQNRFFPATMRAKGLIEAGFLGKVLSFRAS
                     YLHSGSVDPERPMGWKLEKSAGGGVLADLGSHILDLIYFLLGKYTEVFAYNRVLYPKR
                     PHPSGETVEVEAEDLSLLIAKMEDGSVGTIESSKIATGTNDELVFEIHGDKGAMRFNM
                     MDPNWLEVYDNTLPETDLGGDRGFKKIECVQRYNSPGGKFVAPKAPIGWIRSHVHSLY
                     NFLDNVYNLKPSVPSIEDGAYIQYVMDMAQKSHRTKQWVSLD"
     gene            complement(2468..3901)
                     /locus_tag="VFD89_08150"
     CDS             complement(2468..3901)
                     /locus_tag="VFD89_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZJ58192.1"
                     /translation="MKRFQDKNWKEEMRITFDYNHMMSEFVGQEGIEWDEIQALEADL
                     KKASLAMIEKREAGQMKWRELPYNQEEIVQDILETAAGIKDRFDNFVILGIGGSALGP
                     IAVQQALNHMYYNELSKEQRGGRPRLYIIDNVDPQRMEELFDIIDVEKTVFNVITKSG
                     STSETMAQFLIVTGMLKAKVGSDYREHIIATTDRENGSLIQIAKDAGFKTYYIPEGVG
                     GRFSELSPVGLLAAAVCGIDIEEMLAGATYMDEIASQPDTYKNPAYMAATLQYLAMKA
                     GKNISVMMPYVDSLKYMADWYSQLWAESLGKRYDLEGNEVFVGQTPVKALGVTDQHSQ
                     VQLYTEGPFDKVITFLGVKEYKGEVKIPNGYKDHPAVFFLTGHTLNELIHAEQTATEY
                     ALLKAKRLNRTITLPEVNPFTIGQLLYFLEIETAFAGELLGIDAFDQPGVEEGKNATY
                     ALLGRSGYEEKKAELDSRPKKKGKYII"
BASE COUNT         1085 a          996 c          813 g         1146 t
ORIGIN      
        1 atttatagta gtattaatat agatggagga agatagatgt gaaaaactta aagggtacaa
       61 aaaccgcaga aaatctaatg aaggcctttg ccggggaatc ccaggcccgc aatcgctact
      121 ttatgtatgc aagcgttgca gggaaagagg gatacaagca gatcgaagaa attttcctcg
      181 aaacagcaga taacgaaaga atccacgcca ataggttctt taagtttctg gtggaaggtt
      241 ttggggatga acttcccaca accgtagaga taaacgccga ttatcccgtt gccctgggaa
      301 ataccctgga gaatttaaaa gcggctgcag ccggcgagca tgaagaatgg tcggatcttt
      361 atccggcatt tgcccaggtg gccaaggaag agggctttcc ggaggtagcc acagccttta
      421 ccatgattgc aaaggccgaa gttagccatg aagccagatt caacaaacta gccgataatg
      481 taaaggacgg aaaggtcttt aaaaaagatg agcccacaca gtggatttgc agaaaatgtg
      541 gatacataca tacgggggaa aaggctccgg aagtttgtcc tacatgtctc cataaacaac
      601 cccatttcca attgtttaaa gaaacatatt gatgcattag aaggaaatac aaaaggtccc
      661 ctctcacagg gggacctttt gtactttact atttttttct gggtattccc atctccttaa
      721 cgccttgaac cgtattcttt gttcccgaat acagccccga cgccgccaat ccaaaggata
      781 ggcctacaat gatgcccctg gggacatccc tgggatatac atagaatacc cctatcaata
      841 tacccaaaag cgatgctgta agggcactga atttattggg taggcccaga cttttaaata
      901 attccacaac gcctataata aggggaataa tcgctacttg atagatttcc atatccattc
      961 accccatgta attattagtc ccagcatatg taatggtgct attagatata tatgaaggtc
     1021 tattccccgt aataccaaaa ctttggttta aatcagatgg gatgaattgt ttatggatgc
     1081 aaatacgata ttaccccggg cgaaacgacg cccggggtaa tatattcaat tgatctagaa
     1141 atcgattatt cagtccctca gtccagagat acccattgct ttgtcctatg ggatttttgc
     1201 gccatgtcca tgacatattg gatataagca ccatcctcaa tggacggaac ggaaggtttt
     1261 aggttataca cattatctaa aaagttatac agagagtgca catgggagcg tatccaacct
     1321 ataggcgctt taggtgcgac aaactttcct cccggactat tgtagcgttg gacgcattct
     1381 atctttttaa atcccctgtc accgcccaaa tccgtttcag gcagagtatt gtcatagacc
     1441 tcaagccaat ttggatccat catattaaac ctcatggctc ccttatcccc atggatttca
     1501 aacaccagct catcattggt cccagtagct atcttagacg actctatggt gccgacgctg
     1561 ccgtcttcca tctttgctat tagcagggat agatcctcgg cttctacttc caccgtctca
     1621 cccgatggat gtggacgttt ggggtataat accctattat acgcaaaaac ttccgtatat
     1681 ttgcctaaaa ggaagtagat aaggtccagt atatgggacc ctaaatccgc caagaccccg
     1741 ccccctgcgg atttttccag tttccagccc atgggccttt cggggtccac ggatcccgaa
     1801 tgcagatagg aagccctaaa ggaaagaacc tttcctaaaa agcccgcctc tataagcccc
     1861 tttgcccgca tggttgcggg aaagaatcta ttctgaaatg ccatctgggt aattgccttg
     1921 gaagaggcca gcacgtttaa tatctcctct gtctcctggt aggttgcaga taagggctta
     1981 tcacaatata catgcttgcc tgccttcagc gcaccgataa cgtgctcctt gtgcaggtta
     2041 tttggcgtgc atatatttat aatatcgata ttgtcggcag caaatagttc gttggggtcg
     2101 tctgtagcaa attcgaagcc cagatcctct ttagcccttt gggctttttc caaggtccgg
     2161 ttacatatcc ccaccaaccg aatacgaaaa ggcaaatcct cataataaag gggcatagtc
     2221 ctataggcat aggtatgggc cttgcccatg aaaccgtacc caattatacc tatcccaatt
     2281 tccttcaatt cctatctcct cctgtactag ggataaacaa ctcaaactat aaaatttatc
     2341 accatgaaat tgtacgggcc cattgcgatt atcaagccca taagtttagt tgacctattg
     2401 cacctattgc ttatccatat gtattggtta ttacccagaa agcgccatag cgctctctgg
     2461 gtgataatca tattatatac ttaccctttt tcttcggtct tgaatccaat tctgccttct
     2521 tttcctcgta tccggatctg cctagcagcg cataggtagc atttttccct tcctctactc
     2581 cgggttgatc aaaggcatca atcccaagca gctcaccggc aaaagctgtt tctatctcca
     2641 aaaagtacaa cagctgtcct atggtaaagg gattaacctc cggcagggta atggtcctgt
     2701 taagtcgttt tgccttgagc aatgcatact cggtagccgt ttgctcggca tggatcagct
     2761 catttagagt atgccctgtt agaaaaaata cggcggggtg atccttgtac ccgttgggta
     2821 tctttacttc gcccttatat tccttaaccc ccaaaaaggt aattaccttg tcaaagggac
     2881 cttcggtata tagctgtacc tgggaatgct ggtctgtaac acccaatgcc ttgacgggtg
     2941 tttgtcctac aaagacctcg ttaccctcta agtcatacct tttacccaga ctttctgccc
     3001 agagctgtga gtaccaatca gccatatact ttagagaatc cacatagggc atcataaccg
     3061 agatattctt gcccgccttc atcgccagat actgaagagt agccgccata taagcggggt
     3121 ttttataggt gtcgggttga ctggcaatct cgtccatgta tgtagccccg gctaacatct
     3181 cctcgatatc gataccgcat accgcagccg ccagcagtcc cacaggggat agctcggaaa
     3241 acctgccgcc caccccttcc ggaatgtagt aggtcttaaa tcccgcatct ttcgctatct
     3301 gtattaggct cccgttctca cggtccgtgg tggctattat atgttctcta taatcgcttc
     3361 ctaccttagc cttcaacatg cccgttacaa tgagaaattg ggccatagtc tccgatgtgc
     3421 tgccggattt cgtaatcacg ttaaatacgg ttttctctac atcgattata tcaaacagct
     3481 cttccatgcg ctgggggtcg acattgtcga ttatatatag cctgggacgt ccccctcttt
     3541 gctcttttga aagctcgtta tagtacatat ggtttaaagc ctgttgcact gcgatgggac
     3601 ccagtgcgga gcccccaata cctaggatta cgaagttatc gaacctgtcc ttaataccgg
     3661 cggcggtttc taaaatatcc tgaacaattt cctcctgatt ataggggagt tccctccatt
     3721 tcatctggcc tgcttccctt ttttcaatca tggccaatga tgccttctta agatcggctt
     3781 ctaaggcctg gatctcgtcc cactctattc cctcctggcc cacgaactcg gacatcatat
     3841 gattataatc aaaggttatc ctcatttcct ctttccagtt tttatcctga aaccttttca
     3901 cttcaataaa ccccttccca aaattatact gtgatttttc tgtatgaact atatctttaa
     3961 caaaatctgc gttttgtaga aaatacgttt ttctaaatcg ccagatacaa aatccattct
     4021 atcataacaa tataatatat
//
LOCUS       DASRNX010000207        17285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_153290,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000207 DASRNX010000000
VERSION     DASRNX010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17285
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153290"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /gene="gcvT"
                     /locus_tag="VFD89_08155"
     CDS             complement(<1..833)
                     /gene="gcvT"
                     /locus_tag="VFD89_08155"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZJ58193.1"
                     /translation="MKRTPLYEIHTALGGKIIDFGGWALPVQYSGIIKEHNGVRTRAG
                     LFDVSHMGEILVTGDGAGEYIQRMVTNDISNMTAGRVIYSPMCYENGGTADDLLIYKL
                     GEERYLLVINASNTETDFKWLKDNCRNDILSIEDVSEDYGLLAIQGPLSQDILQRLTA
                     QPLGDIRFFRFMQDIEIGGVKALVSRTGYSGEDGFEIYVPSHEAAGLWDKVLDAGSRE
                     GLIPAGLGARDTLRLEAALPLYGHELTSEITPIEAGLTRFVKLKKGNFMGRDALIEQA
                     E"
     gene            complement(920..2092)
                     /locus_tag="VFD89_08160"
     CDS             complement(920..2092)
                     /locus_tag="VFD89_08160"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HZJ58194.1"
                     /translation="MKRAIVIVMDSVGMGALPDAEKYGDEGSNTLGNIAMQIKNFRLP
                     NLEKLGLGNIDGMVGLEAYKDPVGCFGRMGEKSAGKDTTTGHWELMGIILQEPFPTYP
                     NGFPEGVIRDFERAIGSPILGNIPASGTQIIEKLGAEHLETGYPIVYTSADSVFQIAA
                     HEKIISVERLYEICTIARQILTHGHAVGRVIARPFEGELGRFKRTDRRRDFSLDPIHD
                     TVLDNAKKDGLKVKTVGKIIDIFNGRGITHSVHTHDNMDGIDKTIDYIKEDFSGIIFT
                     NLIDFDMLYGHRNDVTGYARALSMLDARIPEIIGALKEDDILIITADHGCDPTFPGTD
                     HTREYVPLLLYGKTLKQGINLGTRDTFADVGASIAEYLEIEGSKEGTSLMNDVRVD"
     gene            complement(2420..3685)
                     /locus_tag="VFD89_08165"
     CDS             complement(2420..3685)
                     /locus_tag="VFD89_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZJ58195.1"
                     /translation="MKLLKLESRLCFNFLWEQVNSDPKSPGYGLILDSTNQPKIASIA
                     SVGFGLSAIPIGIERGWITSAEGYERVLGTLNTLINNVDDHKGFYAHFVDIKTGERWN
                     NCEYSTIDTAILINGAICVGEYFGGKIKELSEVIYKMVDWDFIVDKENNRFYMHYWPE
                     IYPEGHSGLGGQWDYFAEQLMMYILGAGSPTSKLGPELYYRMGKLTGEYKGEIYIRGW
                     YNSLFIHQFSHAWFDSAGYVDRDGVNWFNNSVKATLANRQYCIDNPEGFRTYNSKAWG
                     LTACAGPGGYSGRYGTLPSGIVDEVNNNDGTVPICGPAGSVPFTPQESMDALLHAYEC
                     YPSLWGKYGFYDSYNLDQAEPWFSKEYIGINKGITLLMIENHLSGLVWDLYMKNQFIK
                     DGLKTLMFSEVKKDMGWPDRPMDSPLKTA"
     gene            complement(4426..4719)
                     /locus_tag="VFD89_08170"
     CDS             complement(4426..4719)
                     /locus_tag="VFD89_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58196.1"
                     /translation="MAVRYKREYSLIIGEMVNALKQLDGYFEFLGIGSREWHNLGEEV
                     QGECLKTMSHDIIYGLGTEPVIYVGQGSIKYEPGLAQIRVFDGDKAVYVVRLN"
     gene            complement(4885..5808)
                     /locus_tag="VFD89_08175"
     CDS             complement(4885..5808)
                     /locus_tag="VFD89_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58197.1"
                     /translation="MIVCENLVKIYKTNEIEVVALQGLDLLVEPGELIAIIGNSGSGK
                     STLLNMLGGLDRPSAGNLRVDGKDLLKFSDKELVKYKRETVGFVWQNNARNLIPYLTA
                     QENVELPMMMLKGQQKKHVRAAELLDMVGLGHRRKNKLEQLSGGEQQRVAIAISLANQ
                     PRLLLADEPTGAVDSRTAAQILDVFRDLNSNMGLTVVIVTHDQQISRKVDRVIAIRDG
                     RTSSEFIRRRSYREELEAMESQRDADSQGEDTHEELAVLDRAGRLQIPREYLERLGMS
                     DAKKVKVELEDGKIVLLNPNTRTEEKAANIK"
     gene            complement(5837..6517)
                     /locus_tag="VFD89_08180"
     CDS             complement(5837..6517)
                     /locus_tag="VFD89_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58198.1"
                     /translation="MEPIIRTDDLCRDFPSGREVVHALKEVNISIQPEALTILRGRSG
                     SGKTTLINLLGALDHPTRGDIYLLDKKITDLSEKRRDEIRRTGMGFVFQSIALIGLMS
                     AYENVEFALRVAGYDRKEQKERTEECLDFVGLAKRMNHRPHELSGGEQQRVAIARAIA
                     HYPKVLFADEPTAELDTQMGLQVMKVFRDLVTKEKLTVVMTTHDPNMMELADHVYTLE
                     DGEIVDEI"
     gene            6820..7080
                     /locus_tag="VFD89_08185"
     CDS             6820..7080
                     /locus_tag="VFD89_08185"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HZJ58199.1"
                     /translation="MQKNVGTLDSHARLFIGFSLLGIGIIKSSMLVTGLGAMKIATGI
                     SRFCPILHVLKLTTVGEDNPLDDLLHKIPEYAEHVVDELGEE"
     gene            complement(7132..8418)
                     /locus_tag="VFD89_08190"
     CDS             complement(7132..8418)
                     /locus_tag="VFD89_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZJ58200.1"
                     /translation="MIYYDDCIEGLNGGDQGTRLSSLRRLKEGMDSGKIPRPKGMGYI
                     NNHIHTTYSFSPYSPGKAVWMAFNSGLATAGIMDHDSVSGAGEFIEAGRILGIATTVG
                     IECRVDFSSTPLRGRRINNPDQDSIAYMAIHGIPHTQIQKVTDFFAPYTAERNKRNRA
                     MTRNINRIMEPFDIELDFDREITPISQYGDGGSITERHLLFALSLKLVQKFGRGQELI
                     EFLTRRIGIRIKSPILDHLTDKDNIFYEYDLLGLLKSDLVERIYIDATGECPDVREVI
                     AFSKDIGAIPAYAYLGDVGDSVTGDKKPQKFEDDYLDLLFKVIKDLGFDAVTYMPTRN
                     TMEQLKRVKDLCLSYGFFEISGEDINSPRQSFVCEILNNPIFENLIDSTWALIGHEAA
                     ATDDLSEGMFTDDTASRYPDLHRRIAVYREIGLNLP"
     gene            complement(8460..9755)
                     /locus_tag="VFD89_08195"
     CDS             complement(8460..9755)
                     /locus_tag="VFD89_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZJ58201.1"
                     /translation="MRDSMRYFNISPKIVAEGSESIIKVEPLYGHKGAREDAKYELIH
                     SPMESRGFDETGAHKRVREIIPRGGHFEFSLLFEGEQEHRIEIREILDGGQGAYCEFS
                     IYSLGRDLYPLRPYKGDLHMHSSYSDGRESPAYVAGACRRIGLDFMALTDHGQYGPSL
                     KAQEAYEDLVIDLKILPGEEIHPPDNYIHMINFGGNFSVNDLINAHEDDYYKEVQEIM
                     EKEGADLPEKERYIYASCKWCFERVRQGGGLGIYCHPYWVYENMYNVSNTLHDYIYNQ
                     KPFDAYELIGGFHLHEAESNHLQVAYYQEERAKGVKMPIVGVSDAHGCDTGELFGWYY
                     TVVLSPDLELSSLIGGIKDLKSVAVEALPGSPVRVYGPYRLVKYVLFLIREVFPLHDE
                     LCYEEGRLMLEYIAGNEDAAKILEAYRGRVRGRMDSFWA"
     gene            complement(9884..10834)
                     /locus_tag="VFD89_08200"
     CDS             complement(9884..10834)
                     /locus_tag="VFD89_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZJ58202.1"
                     /translation="MTQGFFIIKSSTVRMTTTNSEYMDFILSDKTGDVNAKWWEYKKE
                     MGDNFIAGTLVKVRGMVTEWQSRLQFRVDRIRVVDEGDDVNPEDFVLTAPMKPEDMLG
                     KVLQYVIKIGNRDIKEIVSRALNEKKDKLMHYPAAMKNHHAIRSGLLYHILTMLSLAE
                     RICQVYPHLDRDLLYGGVILHDLEKINEMDSNELGIVSGYTMEGMLLGHIIQGIKTIE
                     RIGMQVDADPEIVILLQHMLLAHHYEPEFGSPKRPMIPEGEILHYLDIIDARMYDMQK
                     ELDGIEDGQFTNRVWVLHNRQLYRYGKEEDEGKEIGMKAD"
     gene            complement(11150..11638)
                     /locus_tag="VFD89_08205"
     CDS             complement(11150..11638)
                     /locus_tag="VFD89_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HZJ58203.1"
                     /translation="MTSIRTNKKTRFIVEAAALGAVYAVITILLAPISYGQIQVRVAE
                     ALTILPYFTPSAIPGLFVGCIIANIFQGNLMDIIFGSLASLAAGFLSRHMPRSWLVPL
                     PPVIVNGLVIGWVLHVVEGLPYWLTVGTITLGQIVACYGLGYPLFLVLQKNKQKIFGR
                     NS"
     gene            complement(11779..12837)
                     /locus_tag="VFD89_08210"
     CDS             complement(11779..12837)
                     /locus_tag="VFD89_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZJ58204.1"
                     /translation="MGKESMTPRERWIAVLKNQKPDRLPLDYWATPEADHILKRHLNC
                     STSRELYDKLHIDRPIFIGPVYKGPAIPKGQDVYGIVYKDVDYNSGTYSECVYHPLAE
                     FESVDEIEANYTWPEIEWYDYSVIPHQIKGKEAYPIAGGGSEPFLTYKALRGDEQAFM
                     DLVLNPDIVDYCLDKLFGFCYENTARILEMAKGEVTYSYVAEDMGSERDLMFSPAHIN
                     RFLIPRMKKMIDLIHSAGTYVFHHNDGAIRKILPDLIEAGIDILNPIQWRCHGMSRED
                     IKKDFGNDVVFHGGMDNQYTLAFGGVDEVKDEVIDNIKVLGADGGYILAPCHNIQAIS
                     PPENIVAMYETAYEFGWY"
     gene            complement(12941..13735)
                     /locus_tag="VFD89_08215"
     CDS             complement(12941..13735)
                     /locus_tag="VFD89_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HZJ58205.1"
                     /translation="MEQKGMIFDIQRFSIHDGPGIRTTVFLKGCPLRCLWCHNPESQS
                     PLPETGIVNGFGETGAAPRTRVIGREVTTREVMEEVKKDIQYYKNSGGGMTVSGGEPL
                     NQPDFTLSLLELAKDAGIHTCVETCGYAGKDSIQRIIPFTDLFLYDYKETNTARHLEF
                     TGVGNERILSNLRLILENGTSVILRCPIIPGMNDTEEHFLGIVHMERINPELLGIEVM
                     AYHDMGKSKAAQIGKRYELEGIPTVSEELREEWIDRLKGYGCTKVY"
     gene            complement(13746..16013)
                     /locus_tag="VFD89_08220"
     CDS             complement(13746..16013)
                     /locus_tag="VFD89_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HZJ58206.1"
                     /translation="MGEIINNVPELTQKQQIDIMKTFTQTYKTHLGVHPAIREAMCLK
                     VQFPAIMLDIREGDLFAGRLETLPIGFAPQDDGGGVGYYMRENELMDMLAHSSMDPDS
                     RTTVEELMNYWGTETTAYKVRHAYPHAMEKALPSDEWMTEPGIAFPLYRMSGSQLDYG
                     KLLSNGIPGMLGLIEDNIASFLGDRDRLDLFMGMKMAMELLTDTCLHYRDRALKHQSE
                     APDGSWKRQLGVMAETLGNIAKGRPETFREAIQLVFIYCLLSGSNNFGRMDTYLADYY
                     VNDVDSGIITEDEALELLAGMWKLMVVRGKTFDTRVIVGGKGRENEANADRFALLAME
                     ASRIVKEPLPQMSLRFYQGMNPELMEKALSVIGEGRTYPILYNDDVNIPSVMKAFDVE
                     YQEAVDYCPFGCGEYILNHKSFGTPSGVINLLKALEVTLHNGVDPVSGKNMGLALGDF
                     SQFTDFDELFTAYKRQVEYHVEALAKQEELEYIIAGQEAAYLFFSMLFDDCMDRGKAI
                     FSGGVRYLGGTLETYGNINTSDSLAAIKEVVYDKKFITQEQLLEALDADFVGYEDVQR
                     LLSDVPKYGNDDEIADKMAVWVHEHVCNVTRNQRENTNLHSYLVVVINNHANTILGRT
                     TAASADGRKAFTFMANANNPHSGRDTKGITAMLNSLVKLDTDIHAGAVQNMKFSGDLF
                     LKMRDNLKALLKTYFENGGAQAMISVLDRNDLEDAYHHPENHPNLMVRVGGFSARFVE
                     LDRDVQLEILERTIY"
     gene            complement(16408..>17285)
                     /locus_tag="VFD89_08225"
     CDS             complement(16408..>17285)
                     /locus_tag="VFD89_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HZJ58207.1"
                     /translation="YEILVGFSQMIAPFAPFISEEIFTNLTGEESVHLSYFPRMNMEL
                     IDERVEERMDLVRSLVTLGRSARESARIKVRQPLKEIYIDGKYHDLISDLVPLIKEEL
                     NIKEVVFADNLNQYMDFTIKPNFRVLGPVLGPKIRAFQKALSTLDTAVVAPMLDEGKS
                     YTLDLDGEPFEINSDNVLITIAAKEGFNVVMENNLFVILDTAITPQLRDEGYAREFIS
                     KVQQMRKNNDYEVTDNIRIYFDGDEEIASAINHYEDYINNETLALSLERTADTSMERF
                     DLNDHDTGIRIERVQ"
BASE COUNT         4285 a         4525 c         3435 g         5040 t
ORIGIN      
        1 tcctccgcct gctctatcaa agcatccctg cccataaaat tccccttttt aagctttaca
       61 aatctcgtga gtccggcttc tatgggggtg atctccgatg tcagctcgtg cccatagagg
      121 ggtaaagccg cctcaagccg cagggtatcc ctggccccaa gacctgcagg aatcaagccc
      181 tcacgactgc cggcatccaa caccttgtcc cagagcccgg ccgcctcatg ggaagggaca
      241 tatatctcaa agccgtcctc gccggaatat cctgtgcgag agaccagagc cttgacccca
      301 cctatctcta tatcctgcat gaacctaaaa aaacgtatat cacctaaggg ttgcgctgtg
      361 agcctctgca atatatcctg gctcaatggg ccttgaattg ccagcaaccc ataatcctcc
      421 gatacatcct caattgaaag aatatcgttg cggcagttat cctttaacca tttaaaatct
      481 gtctctgtgt ttgaagcatt tatcaccaga agatacctct cctcgcccag cttatagatg
      541 agaaggtcat ccgctgttcc tccgttttcg tagcacatcg gcgagtatat taccctgccc
      601 gctgtcatat tggagatatc atttgtaacc attctttgta tgtattcacc ggcaccatca
      661 cccgttacta ggatctcccc catatgggag acatcgaaca agcccgcgcg agtcctgact
      721 ccattatgtt cctttatgat accggaatat tggaccggta atgcccatcc gccaaaatcg
      781 atgattttgc cccctaatgc tgtgtggatc tcataaagcg gtgttctttt cacattttcc
      841 cctcttcccg atattattgt cttagcagtt acaggtatca ctctatcagc ttttcatcga
      901 aaatccctta aattaggccc taatccactc tgacatcatt cataagactt gtgccctctt
      961 tggacccctc tatctccagg tactccgcga tactggcgcc gacatccgca aatgtatccc
     1021 tggtaccgag atttatgccc tgttttaggg ttttaccgta taacagaagg ggaacatatt
     1081 cccttgtatg atccgtaccc ggaaaagtag gatcacagcc atgatctgca gtaattataa
     1141 ggatgtcgtc ttcctttagg gcgccaataa tttccggaat cctggcgtcc agcatggata
     1201 aggctcgggc atatcctgta acatcgtttc tatgtccata taacatatca aaatctatga
     1261 ggtttgtaaa tataataccc gagaagtcct ccttaatata gtcaatggtc ttgtctatcc
     1321 catccatatt atcatgggtg tgtacggaat gagttatccc ccgtccattg aatatatcga
     1381 taatctttcc cacggtcttg accttgagcc catccttttt ggcgttgtcc aataccgtat
     1441 catgtattgg atccaaagaa aagtcccgtc tcctgtcggt gcgcttaaat cggcccagct
     1501 cgccctcaaa gggcctcgcg ataaccctgc ctacagcgtg tccgtgggtc aatatttgcc
     1561 tggctatggt acagatctcg tagagcctct ctacggaaat tatcttctca tgggcggcta
     1621 tttgaaatac gctgtccgca gatgtataga ctatcggata ccccgtctca agatgctctg
     1681 cccctaattt ttcgatgatt tgggtcccgg atgcgggaat attgcctagt atgggggaac
     1741 caatggccct ttcaaaatcc ctgattactc cttcgggaaa gccattggga tatgtaggaa
     1801 acggctcttg caatatgata cccatcagct cccaatgtcc tgtagtggta tccttccccg
     1861 ccgacttttc tcccatccgt ccaaaacagc ccacggggtc tttataagct tcaagcccga
     1921 ccataccgtc gatattgcca agccccagct tttctaaatt cgggagtctg aaatttttaa
     1981 tttgcatagc tatattccct agggtgttgc ttccctcatc accgtatttt tctgcatcgg
     2041 gcaatgcacc catacctacg ctatccatta caataactat ggcccttttc ataatccgct
     2101 ccttacaaga attatatctt aacaattata tttgcctgtt tccactattt tatcatatct
     2161 ataaaccgat tatatgtcca taacccataa aattcaataa agtgtccgtt taaattttga
     2221 aatagagatt ttcaggatta gtgattaggg gaatctgatg ccagaggcgt aagttaaggg
     2281 gggtgtgcta ctaaaaaatg ggcttattat gatttttagg ggcgcacaca accgtccacc
     2341 cctacagccg aggatactat gatgggatcc gccctattca ttgggtcatg gtgaactcta
     2401 gggaaaaatg tgccaaaagt tatgcggttt tgagcggtga atccatgggg cggtcaggcc
     2461 accccatatc ctttttaact tcgctaaaca tgagcgtctt taatccgtcc ttaatgaatt
     2521 gatttttcat atagagatcc caaacgaggc cgcttaagtg gttctctatc atcaaaaggg
     2581 ttatgccctt gttgattccg atatattcct tagaaaacca gggttccgcc tggtccagat
     2641 tataggaatc gtagaaccca tacttgcccc aaaggcttgg gtaacattca tacgcatgta
     2701 aaagagcatc catggattct tgcggggtaa agggcaccga tcctgccggc ccgcatatgg
     2761 gtacggttcc atcgttattg tttacctcgt ccacaatccc ggacggcaag gttccatacc
     2821 tcccgctata cccgccggga ccggcacatg cggtaagacc ccaggctttg gaattgtacg
     2881 tcctgaatcc ttccgggttg tctatacaat attgtctgtt ggccagggta gccttgacgg
     2941 agttgttaaa ccagttgacg ccgtccctgt ccacgtagcc cgctgaatca aaccaggcgt
     3001 gggaaaactg gtgtataaat agcgaattat accacccccg gatatagatt tcacctttgt
     3061 attcgccggt tagctttccc attctataat acaattcggg acccaacttt gatgtcggtg
     3121 aacccgcccc taaaatatac atcatcaatt gttccgcaaa ataatcccat tggcccccca
     3181 atccggaatg gccttccgga tagatttcgg gccagtagtg catatagaat cgattgttct
     3241 ccttatccac tataaagtcc caatccacca tcttgtagat gacctcggat agttccttaa
     3301 tcttcccgcc aaaatattcg ccgacgcaaa tagcaccgtt tattaaaata gcggtgtcga
     3361 tggtggagta ttcacagtta ttccaccttt cccccgtttt tatatctaca aaatgggcgt
     3421 aaaacccctt atgatcatcc acgttgttta tgagggtatt aagagttcca agaaccctct
     3481 catagccttc ggcggaggtt atccaacctc tttctatacc tatggggata gcactgagtc
     3541 caaagcccac agaggcaatg ctggcaattt tcggctggtt tgtagagtcc aagattaaac
     3601 catagcccgg gctcttgggg tcagagttca cctgttccca taaaaaattg aagcagagcc
     3661 tggactccaa ttttaataat ttcatcccat ccacataaaa caccctttcc caaacaccta
     3721 aatgctttac gacaaatatc ccgcatcacg gcttaggtat cgtaaataat tttttttaat
     3781 ttatatgctt cgaaaagttc attgattgaa ggtaaaaccg gatgcccgtc taaacctttg
     3841 acaacgggtt ctttttatac cttatctaag gactaaaata aaatatagcc aaaattcttt
     3901 tggcttaagt ttcaacgcac gttatataga cccggtatac tacaccggta tactccatgt
     3961 atatggaata ttaattccac aacatcaacc atatcccaat tacacccagc atatcttggg
     4021 attaatttca cccatatgct gtggaatacg gttacttaaa tggattaaaa cttcatatat
     4081 atatcggtat atatgtatag atatgttaac ataatagatg ggctgtatca actgttatta
     4141 tatggttgac ctatcactat gtagccaccc ctttggtggt tgctcaatat gtcgtattga
     4201 tgcagtaatg accctgccaa ggtttatgga tgttgttagg caagataaac acactatcaa
     4261 tgctatatat gttacgaatt aaggataatg ttttacgaca catgtgtacc aatttagaat
     4321 gtttgtcgta aagcatatag caaactgtat atacagtcca attaaatcgc actctaattc
     4381 aaataaatcg caccctaagg tgaggccata ctattaaacc atgccttaat ttagcctgac
     4441 gacatagacg gccttatcac cgtcgaaaac cctaatttgt gccaatccgg gctcatattt
     4501 gatagatccc tgacccacat agattacagg ttctgttcct aatccgtaga taatatcatg
     4561 ggacatggtc ttcaggcatt caccctgcac ttcttcaccg aggttatgcc attcccggct
     4621 tcctatgccc aaaaactcaa agtatccatc caattgcttt agggcattaa ccatctcccc
     4681 tataataagg ctgtactccc ttttatatct cactgccata ttttcaccac ccaaattgta
     4741 gtgttgtttt acgtcagtca tattatacta tgccggagta aacaggacta acaccgaatc
     4801 atatgttctg cggcaggtat cctgcctgtt ttgcataggc ggaccttcgg tagtaataca
     4861 gaacatttat cgccaaatat actattattt gatatttgcc gccttttctt ccgtgcgcgt
     4921 attgggattc agcaggacaa ttttcccgtc ctccagctcc actttcacct ttttagcatc
     4981 cgacatgccc agccgctcca gatattcccg gggtatctgc aatctcccgg ccctgtccaa
     5041 taccgccaat tcctcatggg tatcttcccc ttgggaatcc gcatccctct ggctttccat
     5101 agcctccagt tcctccctat aggagcgcct gcgaatgaat tcgctgctgg ttcttccgtc
     5161 cctaatggct ataacccgat ctacctttct ggatatctgc tggtcgtggg tgactataac
     5221 taccgttaaa cccatattgc tgtttagatc ccgaaatacg tccagaatct gtgctgcggt
     5281 cctgctatcc accgcacctg taggctcgtc cgctaagagc agtctgggtt gattggccaa
     5341 ggaaattgct atagctaccc gctgttgctc accgccggag agctgttcca atttattctt
     5401 tcttctatgg cccaggccta ccatatccaa taattctgcc gccctgacat gttttttttg
     5461 ctgtcctttg agcatcatca taggaagctc cacgttttcc tgggcggtca ggtagggtat
     5521 tagatttctg gcattgttct gccatacgaa acccacggtt tcccttttat attttaccaa
     5581 ctctttgtcc gaaaacttta acagatcctt gccatccacc cgaagatttc cggccgacgg
     5641 tctatccaga ccgcctaaca tattgagcag ggtggacttt ccactgccgc tattgcctat
     5701 gattgcaatc aattctccgg gctctaccag caaatccaga ccctgaagtg ctacaacctc
     5761 aatttcattg gtcttatata tcttaactag gttctcacat acgatcattg gtgaatattc
     5821 cctcccctta cctcggctat atttcgtcta ctatctcccc gtcctctaag gtatagacat
     5881 gatccgccag ctccatcata ttgggatcat gggtggtcat tacgacggtt agcttttcct
     5941 tggtcaccag gtcacgaaac accttcatca cctgcagtcc catttgggta tccaattcag
     6001 cggtaggctc atcggcaaac aatacctttg gataatgggc aatggccctg gcaatggcta
     6061 ccctctgctg ctccccccct gacagttcat ggggtctatg attcattcgt ttagccaaac
     6121 ccacaaaatc aaggcattct tcggtccgct ccttctgttc tttcctatca taccccgcta
     6181 ccctcagggc aaattctaca ttttcatatg cggacatcaa tcctataagg gcaatggatt
     6241 gaaatacaaa acccataccg gtacgccgaa tctcgtcccg tcttttctcg gacaggtccg
     6301 ttattttttt atccaataga taaatatccc cgcgcgtggg atggtctaaa gcccctaata
     6361 aattaattag ggtcgtcttc cccgaaccgg agcgccccct taagatggtt agagcctcag
     6421 gctgtatgga tatattcacc tctttaagcg catgtactac ttccctgccc gatgggaaat
     6481 ccctgcaaag atcgtcagtc ctaatgattg gttccatata ccccacccct gttttctatt
     6541 gggatcctac ggatcctcta taacattata caacaaaggg tcgattatta aaaggaatat
     6601 ggtgaaaata gacaccccca gaaaattcta taataccaca tgtttttcac gttgcgggga
     6661 agaataatgg atggcttttg tagaaacata taagagaagc cctaaaaaac actgttgaat
     6721 ggattgggct atagcgcctt aatgccggtt ttttgaaatt ttaaccggcc gaaccctgtt
     6781 ttaacgatac tcaaataaac tcttaagggg atgaatttaa tgcaaaaaaa cgtaggaact
     6841 ttggattccc atgctcggct ttttatagga ttttccctgt tgggaattgg aattattaaa
     6901 tcctctatgc tggtgacagg cctgggagct atgaaaatcg ctaccggtat cagcaggttc
     6961 tgtcccatat tacatgttct gaagctgacc acagtggggg aggataaccc cctagacgat
     7021 cttctgcaca agattccgga gtatgcagaa cacgtagtgg atgaattggg agaggaatag
     7081 ggggattttt accccctatt ccttaattta catgaaagtc catgaaaccc tttatggaag
     7141 gtttaaccct atctccctgt aaacagctat acgtctatgc aaatccgggt atctgctggc
     7201 ggtgtcatcg gtgaacatcc cttcagacag atcatcggtg gcagctgctt catggccgat
     7261 aagagcccaa gtggaatcta tcaggttttc gaagatcggg ttgtttagga tctcacatac
     7321 gaaggactgg cggggggagt ttatatcctc gccgcttatt tcaaaaaaac cataactgag
     7381 gcatagatcc tttacccttt tcagctgctc catggtattt ctggtgggca tatatgtgac
     7441 cgcatcaaag cctaaatcct ttatgacctt aaacaaaaga tccagataat cgtcctcaaa
     7501 tttctggggc ttcttatccc ccgtcacaga atcccctaca tcgcctaagt aggcataggc
     7561 ggggatcgca ccgatatcct tggaaaacgc gatgacttcc ctaacatcag ggcattcgcc
     7621 agtagcatct atgtatatcc tttcaaccaa atcacttttt aacagtccca aaaggtcgta
     7681 ttcatagaag atattgtcct tgtcggtaag gtgatctaat atgggcgatt ttatccttat
     7741 gcctatcctg cgggttaaaa attcgatcaa ttcctgtccc ctgccaaatt tctggactag
     7801 ctttagagac agggcgaaga gcagatgcct ttcggttata ctcccaccat cgccgtattg
     7861 ggatatgggc gttatttccc tatcgaagtc caattctata tcgaagggct ccattatgcg
     7921 attgatattt cgggtcatgg ccctgttcct cttatttctt tcggcagtat agggggcaaa
     7981 gaagtccgtc accttctgaa tctgggtatg cgggattccg tgaatcgcca tgtaggctat
     8041 actgtcctga tccggattgt ttatccgcct gccccttaac ggggtacttg aaaaatctac
     8101 cctacactct atccccacgg tggtagctat acccaatatc ctccccgcct ctataaactc
     8161 cccggcaccg gaaacggaat catgatccat tattcccgcc gtagccaatc ccgagttaaa
     8221 cgccatccaa acggccttac cgggcgaata aggcgaaaag gaataagttg tatgtatatg
     8281 attgttgatg taacccatcc ccttgggccg aggtattttt ccgctatcca ttccttcctt
     8341 tagccttctc agactgctaa gccttgtccc ttggtcgcca ccgtttaatc cttctatgca
     8401 atcatcataa taaatcatta ttaatttgcc tcctatcggg tacgcagccc atcgcatggt
     8461 taagcccaga agctatccat ccttcccctc accctacccc tatacgcctc taaaattttg
     8521 gccgcatctt cgttacccgc tatatattcc agcataagcc ttccctcctc atagcataat
     8581 tcgtcatgaa ggggaaatac ctccctaatc aaaaacagga catacttaac caatctatag
     8641 ggtccgtata ccctgaccgg gcttcccgga agggcctcca cggctacgct tttcaggtcc
     8701 ttgatgcctc caataaggct tgaaagctct aaatccggcg ataggacgac ggtgtaatac
     8761 cacccgaaca actcaccggt atcacagccg tgggcatccg agactccgac tatgggcatc
     8821 ttcactccct tagccctctc ctcttgatag taggcaacct ggagatgatt ggattcagcc
     8881 tcgtggaggt ggaaaccacc tataagctca taggcatcga agggcttttg attatagatg
     8941 tagtcgtgca gggtattgga gacgttgtac atattctcgt acacccaata gggatgacaa
     9001 tagattccca gccctccccc ctggcgtacc ctttcaaagc accatttaca cgatgcatat
     9061 atgtaccgct ccttctctgg taaatccgcc ccttccttct ccattatttc ttggacttct
     9121 ttataatagt catcctcatg ggcgttgata agatcattta cgctaaaatt cccaccgaag
     9181 tttatcatat gtatataatt atccggcgga tgtatctcct cgcccggcaa tattttaaga
     9241 tcaatcacca gatcctcata agcctcttgg gcttttaggg atggcccgta ttgtccatga
     9301 tccgtaaggg ccataaaatc taggccaatc cttctgcatg ctcctgctac ataggcaggg
     9361 gattctctcc catcggagta gctgctatgc atatgcaggt cccccttata gggtcttaag
     9421 gggtaaaggt cccttcccag ggagtatata ctaaattcgc aatacgcacc ttgccccccg
     9481 tctaagattt cccggatttc tatgcggtgc tcctgctcgc cttcaaatag aagagagaat
     9541 tcgaaatggc cacctcttgg aataatttct cgcacccttt tatgggctcc ggtttcatca
     9601 aaaccccttg attccattgg gctatgtatc agttcatatt tggcatcttc cctagcccct
     9661 ttatgcccgt ataggggctc caccttgata atggattcgc taccttcggc tacaattttt
     9721 ggagatatat taaagtacct catactatct ctcaccgatg tcccctcctc tcgtctttgg
     9781 atttacccgg cggataaatc tcggaaatac aatttaaaag cggattgaaa atcccccttg
     9841 ggtcctacaa tataaggccg cttcgagtat gccttaatag gacttagtca gctttcatcc
     9901 caatctcttt accttcatct tcctcttttc catatctata cagctgtctg ttatgtaaaa
     9961 cccataccct gttggtaaac tgtccgtcct cgattccgtc aagctccttc tgcatatcat
    10021 acatcctagc atcgattata tccaggtaat gtaggatttc cccctccggt atcatgggtc
    10081 tttttgggct tccaaattcc ggttcataat ggtgggcgag gagcatatgc tggagcaaaa
    10141 tgacgatttc cgggtctgcg tctacctgca ttccgatcct ctctatggtc tttatgccct
    10201 ggattatgtg ccctaaaagc attccctcca tggtgtaacc cgacacaatc cccaattcgt
    10261 tggaatccat ttcgtttatc ttctctaaat catggagtat gactccgcca tacaatagat
    10321 ccctgtccaa atggggataa acctgacata tcctttcggc cagggacagc atggtaagta
    10381 tatgatacag aagccccgaa cgaatcgcgt gatggttttt cattgcagcg gggtaatgca
    10441 tgagtttatc ctttttttcg ttcaacgcgc ggctgacaat ctcctttatg tcccggttcc
    10501 caatctttat gacatattgt agaacctttc ccagcatatc ctcgggcttc attggggctg
    10561 tcaatacaaa atcctccggg tttacgtcgt ccccctcgtc tacaacccga attctatcca
    10621 ccctaaattg gagcctgctt tgccattccg ttaccatgcc cctcaccttt accagcgtcc
    10681 cggcaataaa gttatctccc atctcctttt tgtattccca ccactttgca ttgacatcgc
    10741 ctgtcttatc cgacaaaata aaatccatat actcactgtt ggtagtagtc attctgacgg
    10801 tagacgattt tattataaaa aagccctggg tcatcttgcc tacttcaaaa tccccaatcc
    10861 tagtgttttc cacaatcatc ctcctacaat cacacgttgt gttggcccaa tatccataga
    10921 gataatcgcc aaacacccgt gtatctttgg acatgaaagg cccgacagct cgggtataga
    10981 ttataaaatg attccccggc ctaatcgcct ttcgatccat atccatattc atataatacc
    11041 attatttgcc gaaagaataa atgcgttgtt gagaccccag aaaattaacc aatattgtta
    11101 cagtaggggt caatgataat gtgctacccc aaatttccca catataaccc tagctattac
    11161 gcccaaatat cttttgctta ttcttttgaa gtacaagaaa taggggatat cccagcccat
    11221 aacaggctac gatctggcct agggttatgg tgcctacggt cagccagtac ggtagaccct
    11281 ccactacatg aagaacccag cctatgacca gcccattgac gattaccggc ggcagcggaa
    11341 ccagccaaga ccttggcata tgtcgcgata ggaatccggc tgctaaggat gcaaggcttc
    11401 caaatataat atccataagg tttccttgga atatgttggc tattatgcac cccacgaaga
    11461 gccccggtat agccgatggt gtaaaatatg gcaagatcgt cagggcctct gccaccctga
    11521 cctggatctg tccgtagctg ataggtgcca gtagtatggt tataacagcg tagacagcgc
    11581 ccaaagccgc ggcttctact ataaatctgg tttttttatt tgttcttatg cttgtcatct
    11641 atgtcgtttt cctttctctg ctgtgtgtca aggggacggg gtttccgaca catttatcac
    11701 ttttataacc ttgtttaccc atgcctgacc cttcctaaat gattttcgat tcgaaacctt
    11761 accagcgccg tttgatcatc aataccaacc aaactcataa gccgtctcat acattgccac
    11821 aatattctcc ggtgggctga ttgcttgtat gttatggcag ggtgccaata tatatccgcc
    11881 gtctgcccca aggaccttga tattgtcaat gacttcatcc ttaacctcat ctaccccgcc
    11941 aaaggccagg gtgtattgat tatccatacc tccatggaag acgacgtcat tgccaaagtc
    12001 ctttttaata tcctccctgg acatcccgtg gcatctccac tgaatgggat ttaggatatc
    12061 gatccccgcc tcaatcaaat ccggaagtat cttacggatt gcgccatcgt tatggtggaa
    12121 tacataggtc cccgccgaat ggatgaggtc tatcatcttt ttcatccttg ggatcaaaaa
    12181 acgatttata tgggcgggag agaacataag atccctttcg cttcccatat cctcagccac
    12241 atagctgtag gtaacctcgc cctttgccat ctcaaggatt ctggcggtat tctcataaca
    12301 aaagccaaag agcttatcaa ggcaataatc tacgatatcg ggatttaaaa caagatccat
    12361 aaatgcctgt tcatcgccgc gtaaagcttt gtatgttaag aagggttcag aacctccccc
    12421 tgctattgga taggcctcct tcccctttat ctgatggggg atgaccgaat aatcgtacca
    12481 ctcaatttcc ggccaggtat agttagcctc tatctcatcg acactttcga attctgccag
    12541 ggggtgatat acgcattcgc tgtaggtccc ggaattataa tcaacgtcct tgtatactat
    12601 accgtaaaca tcctgacctt tggggatggc cggcccttta tataccggcc ctataaaaat
    12661 gggcctgtca atatgtaatt tgtcgtacaa ttcgcggctt gtggagcaat tcagatgccg
    12721 ttttagtata tggtccgctt cgggagtcgc ccaataatcc aatggcaatc tatcgggctt
    12781 ctggttcttt aagacagcta tccatctttc cctgggtgtc atgctttctt ttcccataac
    12841 acaaacactc tccttctaac tttaatctga cggcccgtac accggctgta cccgtcctta
    12901 gtatgcggtt gttatttgca tataatcatc tggaaaggtc tcagtacacc tttgtacatc
    12961 catagccttt cagcctgtct atccattcct cccgcagctc ttcgctgaca gtgggtatac
    13021 cctcaagctc atatctcttg ccgatttggg ctgccttgct ctttcccata tcatgatagg
    13081 ccattacctc tattcccaat agttcaggat ttattctctc catatggaca atacccaaaa
    13141 aatgctcctc cgtgtcgttc attcccggaa taatggggca tctcaatata acggaggtcc
    13201 cattttccag gataagtctt agattggaca ggattctctc gttgcctaca cctgtaaatt
    13261 ctagatgcct cgcagtgttg gtctccttat aatcgtaaag gaataggtcg gtgaagggaa
    13321 taatcctttg gatgctgtct tttcccgcat acccacaggt ttctacacag gtatgaatcc
    13381 ccgcatcctt tgccaactcc aaaagggata gggtaaaatc cggttgattt aggggttcac
    13441 cgcctgaaac ggtcattccc ccaccggaat ttttgtagta ttggatatcc ttctttactt
    13501 cctccataac ctcccgggtg gttacttctc tgccaattac cctggtacgg ggcgcagccc
    13561 ctgtttcccc gaaaccgtta acgattcccg tctccgggag cggggattgg gattccgggt
    13621 tgtggcacca gaggcacctt aatgggcagc cctttagaaa gaccgtagtc cttatccccg
    13681 gaccgtcatg tatcgaaaac ctttgtatat caaagatcat acccttttgt tccattccgg
    13741 ctcctttagt agatcgttct ttctagtatt tccagttgga catccctgtc aagctccaca
    13801 aatcttgcgc tgaacccacc gacacgcacc ataaggtttg gatggttttc ggggtggtgg
    13861 taggcatctt caagatcgtt cctgtctaaa acgcttatca tcgcttgggc tcccccgttt
    13921 tcaaagtacg tctttaatag ggctttgaga ttatcccgca tctttaaaaa caaatcacct
    13981 gagaatttca tgttttgtac agctccggca tggatgtcgg tatctagttt gaccagggaa
    14041 ttgagcattg cggtgatacc tttggtatcc ctcccgctgt gggggttgtt agcattggcc
    14101 ataaaggtaa aggcctttct cccatcggca gaagcagctg ttgtccttcc caggatcgtg
    14161 ttggcatggt tgttgatcac taccactaaa tagctatgca ggttagtgtt ttccctttga
    14221 ttccttgtaa cgttgcatac atgctcatgt acccatacgg ccattttatc agcaatttcg
    14281 tcatcgttac cgtatttggg gacatcggaa agtagccgct gcacatcctc atagcctaca
    14341 aagtcagcat ccaaggcctc cagtaactgt tcctgtgtaa tgaatttttt atcatatact
    14401 acctctttaa tggcggcgag gctgtcagat gtattgatat tgccataggt ttccagtgtc
    14461 ccgcctaaat atctgacccc accggaaaaa atagccttac ccctgtccat gcaatcatcg
    14521 aatagcattg aaaagaaaag ataggccgct tcctgcccgg caataatgta ttccaactcc
    14581 tcctgcttgg caagcgcctc aacgtgatat tccacttgcc ttttataggc cgtaaaaagt
    14641 tcgtcgaagt cggtaaattg gctgaaatcc ccaagagcca gccccatatt ctttccgctt
    14701 acgggatcga ccccgttgtg aagggttacc tccaaggcct ttagcaggtt tatcacgccg
    14761 ctgggggtgc caaagctctt gtgattcagg atatattccc cgcacccgaa ggggcaataa
    14821 tccaccgcct cctggtattc tacgtcaaat gccttcatta ctgacggtat gtttacgtca
    14881 tcgttataaa gaattgggta ggtccttccc tcacctataa cggatagggc cttttccatg
    14941 agctccgggt tcatgccctg atagaatcgc aggctcatct gaggaagggg ttcctttact
    15001 attctcgatg cctccatggc cagcaacgca aagcggtctg cgttggcttc gttctctctc
    15061 cccttgcctc ccacaataac cctggtatca aaggttttac cccttaccac cattagcttc
    15121 cacatacccg caagaagttc cagggcctca tcttcagtaa ttatccccga atccacatca
    15181 tttacatagt aatctgcgag atacgtatcc attcggccaa aattattgga accggataac
    15241 aggcagtaaa tgaataccag ctgtatggct tctctgaagg tctcgggtcg gccctttgca
    15301 atattgccca aggtctctgc cattacgccc agctgccgct tccacgatcc gtctggtgcc
    15361 tcggattgat gctttagggc tcgatcccga tagtggaggc aggtatctgt cagtagttcc
    15421 atggccattt tcatgcccat aaataaatcc aatcggtccc ggtctcccag gaatgaggct
    15481 atattatcct ctattagccc aagcattccg ggtattccgt tacttagcag tttcccataa
    15541 tccaattggg aacctgacat gcgatacagc ggaaatgcta tcccgggctc tgtcatccac
    15601 tcatcgctgg gaagcgcctt ttccatggcg tggggatagg catggcggac cttgtacgcc
    15661 gtagtctccg tgccccaata attcatcagc tcttccaccg tcgtcctgct gtcaggatcc
    15721 atgctgctat gggctaacat atccataagc tcattttccc gcatatagta cccgacgccg
    15781 cccccgtcat cttggggtgc aaagcctatg gggagggttt ccagcctccc ggcgaataga
    15841 tcgccctctc ttatatccag cattatagcc gggaattgaa ccttgaggca cattgcctca
    15901 cggatagccg ggtgtacccc cagatgggtt ttgtaggtct gggtaaaggt cttcatgata
    15961 tctatctgtt gtttttgtgt cagttccggc acattattaa ttatttctcc cactttttcg
    16021 ttcccccttc agataacccg gttgcatacc accaaactgg aacagccagt cccgggcccc
    16081 attttgctat catcaaatct tacaaatata ctcttcccat taccctatta tgcgggtatg
    16141 gctgttaaag ataaacaata cacaaactaa attcatcggt atggcaatca caatgaccct
    16201 atgcactttc atgaagataa acttggcagt tgaagttatt taacttttaa tagtaggttt
    16261 tcacttaata gtaggtttca ctgacaaaag acacacccgt aaagcaacac ccttatttaa
    16321 aataccccaa aggggacata agccaccttt ggggtaatag tctccttagt tttgttttat
    16381 gacaggatgg tttcgaactg tcctatttta ctgtactctc tctatcctaa ttcccgtatc
    16441 atggtcgttc aggtcaaacc tctccatgga cgtatccgcc gtcctttcaa ggcttagggc
    16501 cagggtctcg ttattgatat aatcctcata gtgattaatg gcgcttgcaa tctcctcatc
    16561 cccgtcaaag tagatgcgga tattatccgt tacctcatag tcgttgttct ttctcatctg
    16621 ctgaactttg gagataaatt cccttgcata tccctcatcc ctcagctggg gggtgatggc
    16681 ggtatctagg attacaaaga ggttgttctc cataactacg ttaaaccctt ctttagcggc
    16741 gatggtaatc aatacgttat cgctgtttat ttcaaagggt tccccatcaa ggtcaagggt
    16801 atagctctta ccctcatcca gcattggagc aacaactgca gtatccagcg tggacaaggc
    16861 cttttgaaac gctctgatct taggcccaag gaccggtcct aggaccctga agttgggctt
    16921 tatggtaaag tccatatatt gattcaggtt atcggcaaac accacttcct ttatattcag
    16981 ttcctccttg atcaggggca ccagatccga gattagatca tgatacttac cgtcgatata
    17041 gatctcctta aggggctgac gtaccttgat ccgggctgat tccctggcag aacggcccag
    17101 agtcaccaga ctccttacca gatccatcct ttcttctacc ctttcgtcta ttaactccat
    17161 attcatcctg gggaagtagc tgaggtgaac ggattcctct ccggttagat tggtgaatat
    17221 ctcctcggat ataaagggag cgaagggggc tatcatctgg gaaaacccta ccaatatctc
    17281 atagg
//
LOCUS       DASRNX010000208         6363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_153510,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000208 DASRNX010000000
VERSION     DASRNX010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153510"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1640
                     /locus_tag="VFD89_08230"
     CDS             <1..1640
                     /locus_tag="VFD89_08230"
                     /inference="COORDINATES: protein motif:HMM:NF017043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58208.1"
                     /translation="NPLYYMGWVMLLPLVYLGFKLHRRRQIFLIIERIRGEWGRELKK
                     DHKFEDISRLYRGMDRIDESQLTIDDQTWHDLNMDSIFKKIDRTTTSFGQQILYSMLR
                     IPLLEKRKLLKRHEIINLFQKNTGIREDILLRLNRLNSSDRGVLYNLIWGEASPDSGW
                     KWIYYTFPVFLLGTLVLIPFMGAIALIRITMPDFMMNIYLYYRAKAKMKVYLDSISSL
                     SSFLVIAHEIGNMKLDEPILEEYIAGLKSACIHCRRIMKKTRKLQKLESRDPFGLNEY
                     IKMLSFMDIRALFAVLEDLRINREHLQKIYKTLGKLDALLSIASFRAGLSRYCIPNFS
                     SGEDWIEIQEMEHPLIQNAVPNSVRLEKNCGLIVTGSNMSGKSTFMRTIGVNAIFAQT
                     IYTCLADSYGGGFFRIMTSISQEDNIVGGKSYFLSEAESLLRIIKGLNDKIPTLCIID
                     EIFRGTNSEERIGAAQRLLEYMVKQNAAVIVATHDLELTQIDNPSYRCYHFSEKVGQT
                     GLEFDFKLKKGVSTTRNALKILGHLGYPSEIASRRVK"
     gene            1973..3496
                     /locus_tag="VFD89_08235"
     CDS             1973..3496
                     /locus_tag="VFD89_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HZJ58209.1"
                     /translation="MKERTYIAIDLKSFYASVECIERGLDPLTTNLVVADASRTEKTI
                     CLAVSPSLKAYGIPGRPRLFQVVQRVKEVNALRLDKASGGVFSGSSCSDMELKSSSDL
                     SLDYIVATPRMAYYIEYSTRIYDIYLKYIAPEDIHVYSIDEVFMDVTNYVDTYNLTAR
                     ELATRILLDVLKTTGITASCGIGTNLYLAKIAMDILAKHTPIDKNGVQIAELDEMTYR
                     KQLWSHRPITDFWRVGKGYAKRLEKHGLYTMGDIARCSVGKENEYYNEDLLYKLFGVN
                     AELLIDHAWGWEPCTIADIKAYKPSAKSVGSGQVLQSAYTFDKVKVVVWEMADSLALD
                     LVDKRLLTDQIVLTIGYDSENLTNPDMKGLYKGEITTDSYGRAIPKSGHGTINLDEYT
                     SSARLICDATLDLFERIMDKDLLVRRVNISANRVVDETSVQKQDRYEQLDLFTDYIGE
                     EKIKEEEEAELMREKKIQETILDIKKKYGKNAVLKGTNLEDVATGADRNKLIGGHRA"
     gene            3493..3903
                     /locus_tag="VFD89_08240"
     CDS             3493..3903
                     /locus_tag="VFD89_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58210.1"
                     /translation="MTGEYDDIMHLLRHVSNTRPQMEITNRAAQFSPFAALAGHGDAI
                     EETARITEKQIELDEYMKRALNDKLQILNERILERPEIRITHFQADETKDGGAYIDTK
                     GIIKKIDEYERIILMEDGIVIPIHGIIDIAGEGI"
     gene            3959..5050
                     /gene="ychF"
                     /locus_tag="VFD89_08245"
     CDS             3959..5050
                     /gene="ychF"
                     /locus_tag="VFD89_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZJ58211.1"
                     /translation="MRLGIIGLPNIGKSTLFNAITKAGAQAANYPFCTIEPNIGMVTV
                     PDERLEVVRKIYGSKEIIPTTIEFVDIAGLVKGASRGEGLGNKFLANIREVDAMVHIV
                     RCFECTDIVHVDGSIDPVRDIETINLELIFADMDSVDRQIERTQKAAKSGDPRLKEEL
                     GLLKYIKANLEEGIAARNMEFDHSQVPFVRQLFLLTSKPVIYVANILEADIGSDNIPL
                     VDDLIESVKGEETEVLKICAGLEEELAELEDDEKASFLSELGIEESGLDKLIKSSYSL
                     LNLISFMTTRPQDVRAWTIKRGTKAPEAAGRIHTDFERGFIRAETIAYEDLVKAGSNT
                     AAREKGLIRLEGKDYEVQDGDVILFRFNV"
     gene            5256..6056
                     /locus_tag="VFD89_08250"
     CDS             5256..6056
                     /locus_tag="VFD89_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZJ58212.1"
                     /translation="MAKDLVKEIREFNRGDVACWWLGQMGFVFKLGNKTLYIDAFLTD
                     LKGRNIKPLISPEEVEADYILGTHDHIDHIDRPAWREIVKHNHAVKFIVPGLLRESVV
                     EDLGIEPERVIAANEGERIVLGDISISPIASAHEFLDQDEETKQYPYLGYIVEYDNFK
                     IYHSGDTCMYPGLIEKLKGFGVLDLAILPINGRDGTRYRANCIGNLTFQEAIDLSGYI
                     RPGCVIPAHYEMFDSNSEDVNKFSDYIEAKYPSQRYLICEHGKVEIFR"
BASE COUNT         1992 a         1176 c         1571 g         1624 t
ORIGIN      
        1 ccaatccctt atactatatg gggtgggtaa tgctgcttcc cctggtctat ttgggtttta
       61 aattacatag aagacgacaa attttcctca ttatagaacg cattaggggg gaatggggca
      121 gggaactaaa gaaggatcat aaatttgaag atattagccg cctgtatagg ggtatggacc
      181 gcatagatga atcacaattg acaatagacg atcaaacctg gcatgatctc aatatggata
      241 gcatattcaa aaaaatagac agaaccacca catcatttgg acagcaaata ctctatagta
      301 tgctccgaat tccgctttta gagaagcgga agctactgaa acgtcatgaa atcatcaatc
      361 tatttcagaa aaatacggga attcgggaag atattctgtt aaggcttaat aggctgaatt
      421 cttccgatag gggggtcttg tataacttaa tttgggggga ggctagcccc gatagtggct
      481 ggaaatggat atattatacc tttcctgtat ttttgttagg gactttggtg ctaatacctt
      541 ttatgggggc aatcgcactt atccgcatca ccatgccgga cttcatgatg aacatatacc
      601 tatactatcg cgcaaaggcc aaaatgaagg tatatttaga ttccatcagc tccctgagct
      661 catttttggt tatagcccat gaaataggaa atatgaaact ggatgaaccc atcctggaag
      721 aatatatagc cggattaaaa agtgcctgca ttcactgcag acgtattatg aagaagacac
      781 gaaagctaca gaaattagaa tctcgggacc cttttgggct gaatgagtac ataaagatgc
      841 ttagtttcat ggacataaga gcccttttcg ccgtattaga ggatttgagg ataaataggg
      901 agcacctaca gaaaatctat aaaacattag gtaaattaga tgccctgctg tcaatagctt
      961 cattccgcgc cggattatct agatattgta taccgaattt ttcatccggc gaagattgga
     1021 ttgagataca agagatggag catcccctta ttcagaacgc agtacccaat tccgtccgtc
     1081 tagaaaaaaa ttgtggacta atagtgacgg gctccaatat gtcaggtaaa tctacattta
     1141 tgaggaccat cggagttaat gcgatctttg ctcaaaccat ctatacctgt ctggccgata
     1201 gctatggggg agggtttttt agaatcatga catccattag tcaggaagat aacatcgttg
     1261 ggggtaaaag ctattttcta tctgaggccg agtccctcct aagaattatt aaaggattaa
     1321 atgataagat cccaacctta tgtattattg atgaaatttt tagggggact aattccgagg
     1381 aaagaatagg cgccgcgcag cgcctgttag aatacatggt gaagcagaat gccgctgtca
     1441 tagttgccac ccatgatctt gagttgacac aaattgataa tccttcctac agatgctatc
     1501 actttagcga gaaagtagga caaacaggat tggaatttga ttttaagtta aaaaaaggag
     1561 tatccaccac ccgaaatgcg cttaaaattt tagggcactt aggatatcct tccgaaattg
     1621 cgagcagaag ggttaaatag aatgtaaaaa ctacttatga agtttaaggg catagagctt
     1681 gttataataa tatccgctcc tattatattt tgtatatggc taaataacag tataattatt
     1741 atggggtgcg attatatcaa ctccaaaatc ccggaagact ttcacgactt tgtgatagcc
     1801 ccttttatac ctttgcaact ttgccttaat gagtgataga ataaagatgc caaagggcat
     1861 gactcgggat ccatcggaca aaaagtgaaa ggactctatc ctataagcgt taggatatgg
     1921 tggaggggtt cattatgcaa atatgtctgc aggaggtgat tatggaaaaa ccatgaagga
     1981 aagaacttat atcgccatcg atctaaaatc cttctatgct tccgttgagt gtatagagcg
     2041 cggccttgac ccgcttacta caaatttggt tgtcgcagat gcgagtcgta cagaaaaaac
     2101 catatgcctg gctgtttccc cttccctcaa agcatatgga atccccggca gaccgagatt
     2161 gtttcaggtc gtacaaaggg tcaaagaggt taatgcattg agattggata aagcgtcggg
     2221 aggggttttt tccggttcgt cctgtagtga tatggaatta aaatcatcat cggacctctc
     2281 attggattac atagtagcca cacctagaat ggcctattac atagagtaca gtacaagaat
     2341 ctatgatatc tatttaaaat atatcgcacc cgaagatatt catgtctatt ccatagatga
     2401 ggtatttatg gatgtcacga attatgtcga cacttataat ctgacggcga gggagctggc
     2461 cactagaatc ctcctggatg tgctgaaaac cacagggatt acggcatcct gcggaatcgg
     2521 cacaaacctc tatcttgcta aaatcgcgat ggatattctg gcaaagcaca cccccataga
     2581 taaaaacggg gttcagatag ccgaactaga cgagatgacc tatcggaagc agctatggtc
     2641 acaccggcct ataactgatt tttggcgggt tggaaagggc tatgcgaaaa ggcttgaaaa
     2701 gcacgggctc tataccatgg gggatatagc aaggtgttct gttgggaaag aaaatgaata
     2761 ttacaacgag gacctattgt acaagctatt tggtgtcaac gcagaactgc tgattgacca
     2821 tgcctgggga tgggagccgt gtaccattgc ggacattaag gcctataagc caagtgcgaa
     2881 gagcgtcgga tcggggcagg tgctccaatc tgcctatacc tttgacaagg ttaaggtggt
     2941 agtttgggaa atggcagatt cattggccct tgacttggtg gataaaaggc ttctaacaga
     3001 tcagattgtt ttgactatag gatatgatag cgaaaacctg acaaatccgg acatgaaagg
     3061 gctgtataag ggggagataa ccaccgatag ttatggaagg gctattccaa aatcaggcca
     3121 tggcacaata aaccttgatg aatatacctc ctcagctagg ttgatatgcg atgccactct
     3181 ggatttgttt gaaaggatta tggacaagga ccttttggtc aggagggtca acatatcagc
     3241 aaatcgtgtt gttgatgaaa cctctgttca aaaacaagat aggtatgaac agctggatct
     3301 ctttacggac tatatagggg aagaaaagat aaaggaagag gaggaggctg aacttatgcg
     3361 ggagaaaaaa atacaggaga ccatcctaga tattaagaag aagtacggta aaaacgcagt
     3421 tctaaaggga acgaacctgg aagacgttgc taccggggcg gataggaaca aattgatcgg
     3481 ggggcatagg gcatgacggg ggaatacgat gatattatgc atttgctgcg gcatgtatcc
     3541 aatacaagac cccagatgga aattacaaac agggcagccc agttttcccc tttcgccgct
     3601 ttagccggtc acggtgatgc catcgaggaa accgccagaa taactgagaa gcagatagaa
     3661 ttggacgaat atatgaaaag ggcattaaac gataagctgc aaatattaaa cgaaaggatt
     3721 ttggaacgac cggaaatcag aataacccac tttcaagcag acgaaaccaa ggacggcggc
     3781 gcctacattg atacgaaagg tataataaag aaaatagacg agtatgagcg gattatcctc
     3841 atggaagatg gcatagttat acccatccac gggataatcg atattgcggg ggaaggcatt
     3901 taactgtcta gggatattct tgtgcccata gtaggaaacg gaacactgga ggtaagagat
     3961 gaggttaggg attataggat taccgaatat aggaaagagc acgttattca atgcaatcac
     4021 aaaggcgggg gcacaggctg ccaactaccc attctgtacc atagaaccaa atatcgggat
     4081 ggttacggta ccggatgaga ggttagaggt agtcagaaag atttatggct ctaaagagat
     4141 tatccccact acgatagaat ttgtagatat cgcggggcta gttaagggcg cgagcagggg
     4201 ggaaggcctt ggcaataagt tcttggcgaa catccgggaa gtggatgcca tggtccatat
     4261 cgtcaggtgc tttgaatgca ctgatatcgt tcatgtagac ggaagcatcg atcctgttag
     4321 ggacatagag actattaatt tagaactgat atttgcagat atggatagcg tggacagaca
     4381 aatcgagaga acccaaaagg ccgccaaatc cggggatccg agactcaaag aggaattagg
     4441 gcttctaaaa tatataaaag ctaacttgga ggagggaata gccgccagga atatggaatt
     4501 tgaccatagc caagttccct ttgtccgcca actatttttg ctcacatcta aaccggttat
     4561 ctatgttgcc aacattttag aggcggatat cggcagcgat aatatacccc ttgtggatga
     4621 cttaatagaa tctgtaaaag gggaagaaac ggaagtccta aagatttgtg cgggcctgga
     4681 agaagagtta gcggaattag aggacgatga aaaggccagc tttttatcgg aacttggtat
     4741 agaggaatcc ggtctcgata agctgattaa aagctcgtac tcacttctta acctaattag
     4801 ttttatgaca acccggcccc aagacgtgag ggcctggacc ataaaaaggg gtactaaagc
     4861 tcccgaggcg gcgggaagga tccatacgga ttttgaaagg ggatttataa gggctgaaac
     4921 catagcgtac gaagaccttg taaaggccgg atcaaatacg gctgccaggg aaaagggact
     4981 gataaggctg gaaggaaagg actatgaggt tcaagacggg gatgtaatcc tattcaggtt
     5041 caatgtctag gttgcccgat tttcttggcc ttaaagcttt agtgattaga tcattattct
     5101 taaaatttag attatgcatc cctggataat aggcagttga taggaagatg atatcgctga
     5161 ttaatagaaa tagtccgctg caataaaatc ggatagcttt ttaaaaggta tcgaaaaaat
     5221 aactataaac aataattcta agaggagcga tgaggatggc aaaggatctt gtaaaagaaa
     5281 tcagggaatt taaccggggg gatgttgctt gttggtggtt gggccagatg gggtttgtat
     5341 ttaagctggg aaataagacc ctatatatag atgctttttt gacggatcta aaagggcgaa
     5401 acattaaacc tctaataagc ccggaagagg tcgaagcgga ttatattctc ggaacccacg
     5461 accatatcga ccatatcgac agacctgcct ggcgcgagat tgttaaacac aatcatgcgg
     5521 ttaagtttat agtcccggga ttattgaggg aaagcgtggt agaggatctg gggatcgaac
     5581 ctgaaagggt catagccgcc aatgagggcg aaaggatagt tttgggtgat atatctatct
     5641 ctccaatcgc ctctgcccac gaatttttag atcaggatga ggaaacgaag caatacccat
     5701 atctcggata tatagtagaa tatgacaact tcaaaatcta tcactcgggg gatacttgca
     5761 tgtaccctgg gcttatagaa aagttgaagg gattcggcgt actggatctt gctatactcc
     5821 ccatcaacgg tagggacggg accagatata gggccaattg cataggaaat ctcaccttcc
     5881 aagaagccat agacctgtcc ggctacatca ggccaggctg tgttatcccg gcgcattatg
     5941 agatgtttga ctctaacagc gaggatgtca ataagttttc cgattatatt gaagccaaat
     6001 atccatctca aaggtaccta atatgcgagc atggtaaagt ggaaatattc cgttgaacat
     6061 acctataaga atctaaatta gacgcgttgt gccagctcat taacctactg agcgataaag
     6121 gtgatgaact actaaagagc gcatgacttc attggttggt agtttggatt ttggaagtta
     6181 gaggttagat tttggaagtt gggagttagg ttctgcgaaa ctgttaccag ggggatggat
     6241 ttgtacaggc agattccaac ggcccgaaag aaggacccgt aagggaaggg gcagaatttt
     6301 tgccccttta ttttgttaca cgcccatgtc aaatatcggc gtctgtagcg aaggcctccg
     6361 agc
//
LOCUS       DASRNX010000209         5156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_726859,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000209 DASRNX010000000
VERSION     DASRNX010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726859"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /gene="ffs"
                     /locus_tag="VFD89_08255"
     ncRNA           <1..238
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFD89_08255"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            427..2286
                     /gene="dnaX"
                     /locus_tag="VFD89_08260"
     CDS             427..2286
                     /gene="dnaX"
                     /locus_tag="VFD89_08260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZJ58213.1"
                     /translation="MAKVGDPVEYTALYREWRPGTFDEVVGQDHVVRILKNQITTHRI
                     SHAYLFCGPRGTGKTSTAKIFAKAINCTDPKDGNPCGQCQVCNIIDSENNIDVIEIDA
                     ASHTGVENIREIRDKVKYPPSAGRYRVYIIDEVHMLSTSAFNAFLKTLEEPPEHIVFI
                     LATTEPHKLPSTILSRCQRHDFRRITLGTLMERLRAIANSVGVNVDDQALETIARWSE
                     GGMRDSISLLDQCISFSGSSITENDVLAILGTAGKEFLFDVVDNIMVGDISALLIQID
                     NLMEEGRDVAVFLKDLIYHLRNLLIIKICDDSTGLLDVSQSTLGQYRQQSTRMDQSRL
                     VRSIEILSELDADIKWSTRPRIMLEMTIVKICRPQDGESLEDLMDRVAVLEGQIARGI
                     PAVKVSNTIEPSSSQGPVSDRLSVSQSPDLGKSSYPQPSSSRFIEPNPTDAGNNDKNR
                     GSADGRYKVEDGTDSGTMSVEREETDTIDTGPKKASPENVDYEGVWADVLKRVRKDRI
                     AIFPLLRDTKLQPGSGNKNVVNLIFPDHQGFYVAAIEKEDNSKYIEDLIAQVAGQHFK
                     LKCYTSGDAPVSGQFAIPEPPVIEEDKDEVTEYDIVQRAIDIFGDEYVEVIED"
     gene            2521..2856
                     /locus_tag="VFD89_08265"
     CDS             2521..2856
                     /locus_tag="VFD89_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZJ58214.1"
                     /translation="MARGGFPGMGNMNNLMKQAKKMQEQMMKMQEELEEKTVEASAGG
                     GVVTVMANGKKEIMEITIDPSVVDPDDVEMLQDLIMAAANEALRMAEEMMQGEMGKIT
                     GGMGIPGMF"
     gene            2998..3597
                     /gene="recR"
                     /locus_tag="VFD89_08270"
     CDS             2998..3597
                     /gene="recR"
                     /locus_tag="VFD89_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZJ58215.1"
                     /translation="MSYYIEPIARLVNELSRLPGVGNKTAQRLAFHIINMPEDHVKSL
                     AKAIDEVKGHIKYCKICGNISEEDMCAICRSPMRDPSIICVVEDVRDVMAMERAREFK
                     GLYHVLNGTISPMDGIGPDDIRVKELLHRVNDEGLKEVIMATNPNIEGEATAVYISRL
                     LKPMGIKTTRIAHGLPVGGDLEYADQVTISKALEGRREM"
BASE COUNT         1639 a          917 c         1237 g         1363 t
ORIGIN      
        1 tataacctgc aacccgctat agcaggattg aagtccaacc ctaggctcgc gcttgtaggg
       61 tcatggttcg aataagtgat gaggatggtt tggtcctacg caacggaaac ccatgaaccc
      121 tgtcagatcc ggaaggaagc agcagtaagt ggacattttt gtgtgccgta ggggcgcctg
      181 accggagtta actactcggt taccgcctat gagcttcttg tcgaaggtag gtgcgcggca
      241 gtaatttttt ataagccatg attgtttttt gaatcatggc ttatatttgc ttttgggatt
      301 ttttcattac agttaatata gttttatggt ataataaatg catcggtgcg aagcaagcca
      361 atcggggctt gcccttacct tatgatatga tattatcggt tgataaattc gtctgggcag
      421 aacaatatgg cgaaagtcgg tgatcctgtg gaatatactg ctctatatcg agaatggcgg
      481 cccggtacct ttgacgaagt ggtaggtcag gaccatgtgg tgcgtattct gaaaaatcag
      541 attacgaccc ataggatttc ccatgcctat ctcttttgcg gacccagggg gaccggcaag
      601 actagtaccg caaagatctt tgccaaagcc ataaactgca ctgaccctaa ggatgggaat
      661 ccatgcggcc aatgccaagt ctgtaacatc atagattcgg aaaacaacat agatgtcatt
      721 gaaatcgacg cagcgtccca tacgggagtg gagaatatcc gggaaattcg agataaggtg
      781 aaatatcccc catctgcagg aagatacagg gtatacatta tcgatgaggt tcacatgctt
      841 tcaacctcag ccttcaacgc ctttcttaag accctggagg agcctccgga gcatatcgtc
      901 tttatcttgg ctacgacaga gccccataaa ctgccttcca ctatactgtc cagatgccag
      961 agacatgact ttaggagaat aacccttggt accttaatgg aaaggttaag ggcaatagcc
     1021 aatagcgtcg gggtgaacgt agatgaccag gctttggaaa cgattgcccg ctggtccgaa
     1081 gggggaatga gagattccat aagcctgttg gatcaatgta tcagtttttc cggttcatcc
     1141 ataacggaaa acgatgttct ggccatattg ggaaccgccg gcaaggaatt tctctttgac
     1201 gtagtggata atattatggt gggtgatatt tctgcccttc taatacagat cgacaacttg
     1261 atggaagagg gtagggatgt tgctgtattc ctcaaggatc ttatttatca tctcagaaat
     1321 ctgttgatca ttaagatatg cgatgattct acgggactcc tggatgtttc ccaaagcacc
     1381 ctggggcagt acagacagca atccactcgg atggatcaat ccaggcttgt ccggtctatt
     1441 gagatattat cggaactgga tgcggacatc aaatggagca ccagacccag gattatgctg
     1501 gagatgacca tagttaagat ctgcaggcct caagacgggg aatctttaga ggatcttatg
     1561 gatagggttg ccgtccttga gggacagata gcccggggaa tccccgcagt taaggtatcc
     1621 aatacgattg agccgtcatc ttcacaaggc ccagtctctg acaggttatc ggtttcacaa
     1681 tccccagacc ttgggaaatc gtcatatccg caaccgtcaa gctcccggtt tatagaaccc
     1741 aatcctacag atgcgggaaa caatgataag aaccggggat cggcagatgg aaggtataag
     1801 gtggaggatg gaacagactc agggactatg agtgtagaaa gggaagaaac ggatactata
     1861 gatactgggc ccaaaaaagc atcccccgaa aatgtggatt acgaaggggt ctgggctgat
     1921 gtgctgaaaa gggtcaggaa ggatcggata gcgatatttc ccctgttaag ggatacaaag
     1981 cttcaacccg gcagcggaaa taaaaatgta gtgaacttaa tttttcccga tcatcaaggc
     2041 ttctatgttg cggcaataga aaaggaagac aacagtaaat atattgaaga tttgatcgcc
     2101 caggtagcgg gacaacactt taagttgaaa tgctatacaa gcggtgatgc gcctgtgtcc
     2161 ggacaatttg ctattcccga accgcccgta attgaggaag acaaggacga agtgacggaa
     2221 tatgatatag tacagagggc tattgatatc tttggcgacg agtatgtaga ggtaatagag
     2281 gattaaggag tatcggaaat tagacttttt attacaagtt cagttacact acttgatttt
     2341 gtgtaagata atgtttttag taacatatga attgtggata aagttttacg atgactacag
     2401 ataaacaact taaactataa aatttatcac ttatagataa atttagttcc gtattgttta
     2461 tctgcagata ccaatataga atatctgtcg taagcatata gataagagga ggctataatt
     2521 atggcaagag gtggatttcc cggaatgggc aacatgaaca atctaatgaa acaagcgaag
     2581 aagatgcagg aacagatgat gaagatgcaa gaggagctgg aggagaagac cgtagaggca
     2641 tccgccggcg gcggtgtcgt aaccgttatg gcaaatggca aaaaggaaat catggaaatt
     2701 actatagatc cctcagtggt agacccggac gatgtggaga tgctccagga tttgatcatg
     2761 gccgcagcca acgaagctct gaggatggca gaagagatga tgcaaggcga gatgggcaaa
     2821 attaccgggg gtatggggat cccggggatg ttctagatat caattgatat gcattcccac
     2881 tacacagttt tatagttcta gaagaggtgt ttaaattgct ctctcagtgg aaaggaggaa
     2941 gggcatttcc ccaaagggct tatgccaggg gtttttatgc cctaatttat taggtttatg
     3001 agctattata tagaacctat tgcccgcctt gtcaatgagc tgtcgagatt gcccggggta
     3061 ggtaataaaa cagcccaaag gcttgccttt catataataa atatgccgga agatcacgta
     3121 aagagtctcg caaaggctat tgatgaggta aagggacata tcaagtactg caagatatgt
     3181 ggaaatataa gcgaagagga tatgtgcgcc atatgtagaa gccctatgag ggacccatcc
     3241 attatatgcg ttgtagagga tgtcagggac gttatggcca tggaacgagc acgggaattt
     3301 aagggtctgt accatgtact aaacggcact atttccccta tggatgggat agggcctgat
     3361 gatattcggg ttaaagaatt attgcatagg gtcaatgacg agggtttaaa agaagtgata
     3421 atggcgacaa acccgaatat agagggcgag gccactgccg tctatatatc taggttgtta
     3481 aagcccatgg gaatcaagac cactcggatc gctcatggtc ttcctgtcgg cggtgattta
     3541 gagtacgcag atcaggtaac catatccaag gcattagaag gtcgaaggga gatgtgattc
     3601 tccctttttt tgtcaaatat caaaacaaaa taaacttcac tcaaaaaaaa taaagtcttg
     3661 ttaggctcct tttagggaaa aggaaataag ggataaggat caagtagaaa acgaagaatt
     3721 agacgaagaa aaaaacgaag aattactaga ctaaataacc atcccttaac tgtgataatc
     3781 aaataaagtc gtatcaaaga agataaactc gttcctcaag taaataaagt cgtgtcaaaa
     3841 aaccatagtc aagggatttg aaatgtccat tgaccatggt ttttttgtta gggttgatgc
     3901 cagacaggtt taagatcaga gcaagacaga accaataaac aagccaaaaa gccggatata
     3961 taagggttta gagctggtag aaaactagga tagaacaaag ggggaaagaa aaaggaaaga
     4021 aaaaagaaga ttttgttctt atactactcc catttccccc caaacgaagg aacgacttta
     4081 tttactttta tcgctgccat atttggagtt taatgggaat ggaataagaa aaaaagagtg
     4141 aattggggaa aaaggctaga aaagttaaga aataacaaag gttgatttta agcgggttac
     4201 aggtgatttt tcggaacgag tttatttgcg ttttggcatg agtttatttt gtttacacat
     4261 agttaggatc aagtatttaa gcgaaaaaga ttaaagaaca aaatgaaagt tttgggaaag
     4321 tgctaaagtc gttccaaaat gaaaataaag ttgtatcctt gttaggttaa tcgaggattt
     4381 cttcgtgatt tgtaagccat aataataaat ggaatgactt tatttgcttc ttgatatgac
     4441 tttaacgata caaaattatt tttatttttt caggaatttg ataaagagaa gcaaattaga
     4501 aaaatttttg agtccataaa gttgtgcctt aaatatgtac atgagataaa aaggaggtcg
     4561 agtaacagct gggcttatcc atgtcatggc atagaagatc cttgaaagct caatggaggc
     4621 aagtctattc tagagtaggc atctaaactt ctttcgattt aaagcctata tcaattgtga
     4681 agttactaga attccctgtc cctctccaaa gattaagcag gaagacgtac atggtcacct
     4741 ccttgtctgg atttataatg gtagttgagg tccttattct agagcttgcg attcatatgc
     4801 cgttatatgc aatcgataga ctaattggat aagatggtat aagactatag gaaaggattt
     4861 gcactatata gacaatcctt cacacaaaac tcaacatcta atactttccg tagtttcata
     4921 gaattattct agacttaaat tatagctcac ttgaatttcc ttgggctaat atgcttaata
     4981 tgaaggagag cctagctaat cgctagactc ttatcgatta agtccgtata atggtgtaaa
     5041 tagaaaaatg agttgagcca aagctctacc tcggttaaaa tataagtacg accaaatatc
     5101 aaaccgagga ggaagaacaa tggctcagtt caatattaca ataaccgagg aacttt
//
LOCUS       DASRNX010000210         5389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_522592,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000210 DASRNX010000000
VERSION     DASRNX010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522592"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..785)
                     /locus_tag="VFD89_08275"
     CDS             complement(24..785)
                     /locus_tag="VFD89_08275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZJ58216.1"
                     /translation="MDKLLEGRVAVVTGGGKGIGGGISRALAAQGAKVVLNYNSNPDF
                     AERTVKDIKRNGGEAYTCLVNVENRDQVDAMMQKAVELYGGIDILVNNAAWQPNMDID
                     ECTGEVYDGVININLRGYFRCIQSALPYLKKSTRPRIINISSIHAKRPTDFDVCYAMS
                     KGGIEMLTREAAMELVQYGILINALLPGGTKIEFKSGETQPFKFKRIRRKRQYGFRSI
                     GVPEDTANAVIFLASDMGSHINGTSIRIDKGAILF"
     gene            complement(797..1537)
                     /locus_tag="VFD89_08280"
     CDS             complement(797..1537)
                     /locus_tag="VFD89_08280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZJ58217.1"
                     /translation="MVNGNKVALITDASHPSAASIAEKLESKGIVVVKNYPRLDKKGP
                     MEGKPNSFAFDTWSYDDMNRLLEMIVEKIGNVNYLVHSDNVIFRSTIEDISEDDFKKV
                     LDYNAKSAFMTTKVFAEHMAQNGGGAIVYLSTLHDSKPTGCAFAYSVGKGAVKMLCKE
                     MALFYGRQGVRANVVQMDYIEEQTELLDSHISPFNYDATTKIPLRRLAKPDDSAGIVS
                     FLLSDEAAFINGTDVRVDGGHLLYYLDR"
     gene            complement(1562..2749)
                     /locus_tag="VFD89_08285"
     CDS             complement(1562..2749)
                     /locus_tag="VFD89_08285"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HZJ58218.1"
                     /translation="MITKVNALKRDFESEVQDFLGFWEKYVIHFGEDVFYGIVDSNLV
                     AHPNASKHLVLGARLVWTFSSAYRIFKKDSYKELSKRLYDFFVAHFIDKEHGGAFWEL
                     KADGTPLDTSKRVYGQSFAIYALSEYYRAFRDAEALDLAVSVYNCLETHAYDKDNKGY
                     FEAFSRDWKMDQVEQVSNINPYASASKTMNTHLHLLEAYTSLYRVWKDDVLEDKLKEH
                     IHVMVERIVNHENWHYMLYFDAGWQSQSPDISYGHDIEGSWLLWEAAEIIGDSELEEF
                     VRPVSLKMAEAVFDNGWHPDGGIIYETDPEKGTVQDFRSWWVQSEALVGFYNAWQLGG
                     EDKYLKATLDVYEFIDEELTDHENGGWYPNAISDNRQGNRADGWICPYHNARMYFELI
                     ERV"
     gene            complement(2957..4345)
                     /locus_tag="VFD89_08290"
     CDS             complement(2957..4345)
                     /locus_tag="VFD89_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZJ58219.1"
                     /translation="MKDIHNNYEETLNHVNTFSKPSELKITDMRFANIVGAPFDSILV
                     KLYTNQGLVGYGEVRDGGSKTFAAMLKRMILGENPCNIDKIFRRIKQFGHHARQGGGV
                     CAVELALWDLAGKAYGVPVYQMLGGKFRDKIRLYCDTDVRGKHTGYDMGKALKGRMDR
                     GYTFLKMDLGISELFGEEGTLSAPLGFMDEFQNAGKLAREASKNKKEDPFAWYNARNR
                     QYDMYNIAHPFTGIHITEKGLDRLEEYVAQVREVVGYEIPLAIDHFGHIAVEDCIKLA
                     KRVEKYNLAWAEDMVPWQYTDQYVRLKNSTTTPICTGEDIYLKENFKPLLEQGGVSVI
                     HPDILTSGGILENKKIGDMAQDYGVAMAIHMAESPIACMAAVHSAAATENFLALEHHA
                     VDIPWWEDLVVGLPKPLVKDGFIEVPDKPGLGIEALNDELISQHMHPDNPGLWEPTDE
                     WDNDFSNDRLWS"
     gene            complement(4809..5297)
                     /locus_tag="VFD89_08295"
     CDS             complement(4809..5297)
                     /locus_tag="VFD89_08295"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58220.1"
                     /translation="MAARTKRGGAVIWGGVRDTEQMMAIKNVKTYHRGNDPTGIGDVT
                     LVSYNGPCRIGKAICLPGDVVLGTPAGVLFIPAHLAEECTIGAEKSNIRDIWGFQMLD
                     EGKYSTAQIDAGWTVDMWEDFLGWFGTAPKAKDYQHLTWEKELEHAREIQGKEPKADI
                     RL"
BASE COUNT         1404 a         1319 c         1066 g         1600 t
ORIGIN      
        1 gaaataaatt tggaatttac tcattaaaag agtatagcac ccttatcaat tcgaatgctg
       61 gtaccgttta tatggctacc catatcggag gcaaggaata ttaccgcatt agctgtatcc
      121 tccggaaccc caatggaacg aaagccgtat tggcgtttcc tacggattcg cttgaattta
      181 aagggttggg tctcaccgga tttaaattcg attttggtac ccccgggtaa cagggcgttt
      241 atcagtatac cgtattgtac gagttccata gccgcttccc tggtcagcat ctcaatcccg
      301 cctttggaca tggcatagca tacatcaaag tcggtcggac gtttggcgtg tatcgatgat
      361 atattgataa ttcgcggacg tgtactcttt ttgaggtaag ggagtgcgga ttggatgcaa
      421 cggaaataac cccgcaaatt gatatttata acgccatcgt agacctctcc ggtacattcg
      481 tctatgtcca tattgggttg ccatgcggca ttgttgacca ggatatcaat tccgccgtag
      541 agctccacag ccttttgcat catggcatca acctggtcac gattttctac gttaaccagg
      601 caggtatatg cctcgccgcc atttcgtttt atatccttga cagtccgctc tgcgaaatcc
      661 ggattggaat tgtagttcag tactacttta gcgccctgag ccgctaaggc tctgctgatt
      721 ccgccgccta tacctttacc gccgcctgta actactgcaa cgcgtccttc taataattta
      781 tccattttaa tcacccttat cgatctaagt aatagagcag atggccgcca tctacacgaa
      841 catcggtacc gtttataaaa gctgcttcat cggagagcaa gaaactaacg attcctgcgc
      901 tgtcatcggg ctttgctaat ctgcgaagcg gaattttggt agtcgcatca taattaaacg
      961 gcgaaatgtg actgtccagc agttctgtct gttcttcgat gtaatccatc tggaccacat
     1021 ttgctcgcac gccctgacga ccgtagaata gcgccatttc cttgcacagc atttttacag
     1081 cgcctttacc aacgctatat gcaaaggcac accctgttgg cttactgtca tgcagggtag
     1141 aaagatagac tatcgcccca cccccgtttt gcgccatatg ctctgcaaat accttggtgg
     1201 tcataaatgc actcttcgca ttatagtcta agaccttttt aaaatcgtct tcggagatgt
     1261 cttcgatggt ggagcggaaa attacgttat cgctatggac aaggtaattt acgttcccaa
     1321 ttttctcaac tatcatctcc aaaagcctgt tcatgtcatc atatgaccaa gtatcaaagg
     1381 caaaggaatt gggtttcccc tccatgggtc cctttttatc caatctcgga taattcttta
     1441 ccaccactat ccctttgctc tctagttttt ctgctatgga ggcagctgat ggatgcgagg
     1501 catcagtaat caaagctacc ttatttccat taaccataga tgctttcccc tttcaatagg
     1561 cttatactct ttcaatcaat tcaaaataca ttctagcgtt atgatacggg cagatccatc
     1621 cgtcggccct gtttccctgt ctattatccg atatggcgtt ggggtaccac ccaccgtttt
     1681 catggtccgt taactcttca tcgataaatt cgtacacatc caaggtagcc tttaaatatt
     1741 tatcctctcc gcccagctgc catgcgttat agaacccaac caaggcttcc gattgtaccc
     1801 accatgaacg aaaatcctgt acagtcccct tctcgggatc cgtttcatat ataatgcccc
     1861 cgtcgggatg ccatccatta tcaaaaacgg cttctgccat ctttaaggat acgggcctga
     1921 caaactcttc aagttcggaa tccccaataa tctcggctgc ttcccataat agccagctac
     1981 cttcaatatc atgtccatat gaaatatcgg ggctctggga ctgccagcct gcatcaaagt
     2041 acagcatgta atgccaattt tcatgattta cgattctttc taccataaca tgaatatgtt
     2101 cttttaattt atcttccagg acatcgtcct tccagaccct atagagggaa gtgtaggctt
     2161 ccagaagatg tagatgggtg ttcatggtct tagaggccga agcatatggg tttatattgc
     2221 tgacctgctc tacctgatcc atcttccaat cccttgaaaa agcctcaaaa tagcctttat
     2281 tgtccttatc ataggcgtgg gtttccaggc aattatatac agagacggct agatcaagag
     2341 cctcagcatc tctgaaggcg cgataatatt ccgatagggc atatattgcg aaggattggc
     2401 cataaacccg tttgctggta tcgagtgggg taccgtcagc ttttaattcc caaaaagctc
     2461 ccccgtgctc tttatcaatg aaatgggcta caaaaaaatc ataaagccgc tttgataact
     2521 ccttatatga atcttttttg aatatgcgat aagcagaaga aaatgtccaa accaggcggg
     2581 cccctaaaac aagatgtttg ctcgcattgg gatgtgccac caggttggaa tcaactatac
     2641 catagaatac atcttcgccg aaatgaataa catacttttc ccaaaatcct aggaaatcct
     2701 gtacctctga ttcaaaatcc cgctttaaag cgtttacctt tgttatcata gataatcaat
     2761 ccttttgatt atagtataat atatttatcc gggcaattgt aacgatatca tacatacaac
     2821 atctataccg ggctaaaacc cagcatagat gttgtatgtg tatagatata gtatataatc
     2881 tcagactgat ctggcccgtc tcaagatgac aaacatcaat tagtccggat ctactttaca
     2941 gaaagggaag gaagaattag ctccacagcc tatcgttgga aaaatcatta tcccattcat
     3001 ctgtcggctc ccatagtccg ggattatcag gatgcatatg ctgtgatata agttcatcgt
     3061 ttaaggcttc aatgcctagt cccggtttat caggcacttc gataaatcca tcttttacta
     3121 aaggtttggg cagtccaacc accaaatctt cccaccatgg aatatccact gcatggtgtt
     3181 caagggctag gaagttctcc gttgccgctg cagaatggac ggctgccatg caagcaattg
     3241 ggctctctgc catatggata gccatagcta caccgtaatc ttgagccata tcgccaatct
     3301 ttttgttctc taagatgcca ccggaagtca aaatatccgg atgaattacc gatacgccgc
     3361 cctgctctaa taggggctta aagttctctt taaggtatat atcctcacct gtacatatgg
     3421 gagtagtggt ggaattcttt agcctgacat attggtcggt atactgccac gggaccatat
     3481 cctctgccca ggccagatta tacttctcaa cccgctttgc aagcttaata caatcctcca
     3541 cggcgatatg accgaagtga tctattgcaa ggggaatttc atagcctacg acctcacgaa
     3601 cctgtgctac atactcttcc agcctgtcca gtcccttttc tgttatatgt attccggtaa
     3661 agggatgggc tatgttatac atatcgtact ggcgattgcg ggcattgtac caggcaaagg
     3721 gatcttcttt tttattttta gatgcttctc tggctagttt accggcattt tggaattcgt
     3781 ccataaagcc taggggtgcc gatagggtac cttcttcgcc aaagagttcg cttataccga
     3841 gatccatctt taaaaaggta tagcctctat ccattcttcc ctttagggcc ttacccatat
     3901 catatcctgt atgtttacct cttacgtcgg tgtcgcaata caatcttatc ttgtcacgga
     3961 atttaccgcc cagcatctgg tatacgggaa caccgtatgc tttaccggct aaatcccaaa
     4021 gagcaagttc tacagcacaa acccctccgc cttgtcttgc gtgatgaccg aactgtttga
     4081 tgcgtctgaa tatcttatca atattgcagg gattttcccc gagtatcatt ctctttaaca
     4141 tcgcggcaaa agttttactt ccgccatctc tgacttcccc ataccctact aaaccctgat
     4201 tggtgtagag tttcactaag atgctatcaa aaggcgcacc aactatatta gcaaatctca
     4261 tatcggttat cttaagttca gatggcttgg agaaggtatt gacatggtta agggtttctt
     4321 cgtaattgtt atggatgtct ttcatagctt gtatcctcct gtgagagctg tcattttcca
     4381 taaagctcat tctcaatttt gatattatgg gattttaccc tagaaaacat tttatagata
     4441 aactactcca actataagac ttatcaacta cagataaatt tagttttcat attgtttatc
     4501 cataatcagt tcatatatac aacgccttat accgagcaaa gtttgcccag tatagacgtt
     4561 gtatataccc taaaatactt tgaaacatcc gaagcaattg cattggcaat tgcaacacac
     4621 gagtttcaac gaggcgggtg atttataccc aatgcccctg ttttactgat gggaacccgc
     4681 tgcctcgtta taacatcgga agcaattgca acacacaggt ttcaacgagg cgagtcattt
     4741 atacccatcg cctattttac taatcggaac tcgccgctta tagaagcggg agatattttg
     4801 gcgcctcgtt ataatctgat gtcagctttc ggctctttac cttgtatctc acgggcatgt
     4861 tctaattcct tctcccatgt gaggtgctga taatctttgg ctttgggagc ggttccaaac
     4921 cagccgagga agtcttccca catatccact gtccagcctg cgtcgatttg agctgtggaa
     4981 tatttcccct cgtcaagcat ctggaatccc caaatatctc tgatattgga tttttcggct
     5041 ccgatagtac attcttcagc taagtgggcg gggatgaaca atacgcctgc aggggtacca
     5101 aggacaacat cgcccggcag acagatggct ttacctatgc ggcaaggtcc gttatagctg
     5161 accagggtaa catcgcctat acctgtagga tcgttaccgc gatgataggt tttgacgttc
     5221 ttgatagcca tcatctgttc agtatctcgt accccacccc agattacggc tccacccctc
     5281 ttggtgcggg cagcgatggc cgttgtgagg tttccaccta caaaggttcc ttgatagctt
     5341 tatcaaacat atcaatgaca acaacatcgt cttcaactag ggagtcgat
//
LOCUS       DASRNX010000211         9173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_523532,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000211 DASRNX010000000
VERSION     DASRNX010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9173
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523532"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..385
                     /locus_tag="VFD89_08300"
     CDS             164..385
                     /locus_tag="VFD89_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58221.1"
                     /translation="MTTTITEHLKSIATYFVALIFVVSGVGLFLFYSAEKSGKEFTTE
                     ATIAKYGAMIYVISGIGIAVIKIFLQILS"
     gene            complement(395..1525)
                     /locus_tag="VFD89_08305"
     CDS             complement(395..1525)
                     /locus_tag="VFD89_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HZJ58222.1"
                     /translation="MVRKGILTSLIALSTIFAVGCWDRVEIEKNAFILGLGIDLEEDN
                     SLNITYQVALLDSFKGEKGEKSKSTQQISIKSNSLSESRDALLKHFGDTPNLSHCKLI
                     VFGEEYARNGIKSSLDYLFREPDIRRITSVCVAKGRAKEVLDSEPQTAPSSAMVIDQI
                     VTQNSANNSGIFPFPDIGYLHRNFIRESDVALVGINAGKDTVEVSGAGIFKDYKLVGW
                     FSDSEVIGQRFILGEVHKGLLTADLSYEMGDRLTLNAYNINVRTIPKIKDGKIKIETK
                     VFIEGDINEVGNPTTKLDTKTLLEFWGKAVENKIRDTVDAAYKKGRDTYGVDCFEIDE
                     KIEGYYPKFWKEHGKEWNELFKTADLDLEIKVKIRRVGLIEP"
     gene            complement(1518..2615)
                     /locus_tag="VFD89_08310"
     CDS             complement(1518..2615)
                     /locus_tag="VFD89_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HZJ58223.1"
                     /translation="MVKKHDKLGGLQYISLISTTILGVIVISLPKIAAESAQEGALLS
                     TFLAGVLVAIFAVSMNILGRRFQKKTVIEYLVSLLGKPLGKFLGVILIIYFMFSTSLV
                     LRGFSDALKGLLLHSTPLEVIMVSMLFTAVYLSLNGINAIAKISELFLPIIILSLILV
                     IGLSINSFSYVRLRPVLFPPLWRTVKGMPNVITAFLGYEIILLIIPFVERKKADIPRT
                     LIGIAIPTVLYILLVIMAVGAFGLRPSQQINYPTIALARIINFPGAFADRFDIFFVIL
                     WILAAFTSVANLLYISSLSTVRLFGLRNYQTFIYILTPIVYMLAILPQDLPEIRKVNT
                     YVGYLGAGISMLVVGLLIFALVFGKKEHENG"
     gene            complement(2608..4212)
                     /locus_tag="VFD89_08315"
     CDS             complement(2608..4212)
                     /locus_tag="VFD89_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HZJ58224.1"
                     /translation="MKITKKDRNPKNREVMEENDPPQLKAEEKVDVTLSNSTEENLEK
                     FKAFLGDSSDAVFREFRLGSQGIPCALVFIDGLVNSDVINKNILKSLMYEITVLERAR
                     NESIQVESAYDFIKQNAVAVGEIAEASTLDKCMLMVMSGEVALIVEGSEKILIVNARG
                     WPARDVSEPVTEGSVRGPKDAFTETLRVNTALLRRKCKDPNMVIKTIQLGRRSKTDVS
                     YVYVKGITDPGLIEEVEKRLNQVDVDEIMETGQIEQWIQDSTLSPFPQVQLTERPDTT
                     VSNLLEGRVAILVDNSPFAIIVPAGLYQFFQAPEDYYDRWIIGSFLRFLRWSASLLAA
                     FVPALYIAVITYHPSFLPTPLALAIAANRVNIPFPAFVEAFLMEVTIELLRESGARLP
                     KAIGQTIGIVGGIVIGDAAVRAGITSPSMVLLVAITAIASFVIPTYSAGIALRLIRFP
                     LMILAAILGLYGVALGFILLNIHLVSLKSFGFDYMTPQAPVVPQDTRDWILRLPRNLL
                     WRRPISVHPVDIDRMDQDPYKENGNG"
     gene            complement(4434..5765)
                     /locus_tag="VFD89_08320"
     CDS             complement(4434..5765)
                     /locus_tag="VFD89_08320"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HZJ58225.1"
                     /translation="MAEIIPFKALRYNVDIVGDVSDVVTPPYDIISSEEQEGFYNVNP
                     YNSIRLELPKDLPGDSEHDNKYTRAGEILNSWQDQGVLLTEEKDCLYIYQQEFKLGDQ
                     ISRVRTGLIGLIRLEEFDKGIILPHENTLSKPKADRLQLMRACNANFSQVFGLYDDHK
                     RRVPSLIDAYCSKHKPDILADGGPIETKERVWAVDDPQFIGDIREALKGEKLYIADGH
                     HRYETALAYRNELRDKNPNHTGDELYNYVMMTMVDMEDSGLVILPTHRIVNGICNFSI
                     DSLIDSLGDQFYIEEYDGAAFDIKEKSNIIQNILDEKGLHTFVLYVGSGHPIYALSLK
                     SMDMMKTLHPKRHSSYLDLDVSILHSLILDRILGIGEKELANQENLEYTHNIMEGIEE
                     VEHGNHQMTFFISSTAVRQIQEVSLANEKMPQKSTYFYPKLITGLVFNKFR"
     gene            complement(5872..6489)
                     /locus_tag="VFD89_08325"
     CDS             complement(5872..6489)
                     /locus_tag="VFD89_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58226.1"
                     /translation="MPPQKFNPEEYLTKLPLRRRNPKTNSWETQFADYLEIKWRMVWF
                     RENNFENTQTIVLDKIIDIDNRFAYFELQVTDTKGNVEVGIGSETGEDFRDYIEKAYT
                     KAYGRALAALGYGTQFAPDLEEGERIADSPVAGSNHSDFQEQDDNITDPQRRAIFAIY
                     TSLNLGEDEMKDLISSRYQKVDSKALTKREASDLIDYLNNLKELQ"
     gene            complement(6580..6984)
                     /locus_tag="VFD89_08330"
     CDS             complement(6580..6984)
                     /locus_tag="VFD89_08330"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HZJ58227.1"
                     /translation="MSIKWQNSWNIEVGQIDNQHKALVEQVSRLLEACRKGGSKQEVA
                     NALNFLSRYVREHFQYEEEQHRIHNYPDRDAHARLHNTFTHEVERLYRRFEEEGVSIQ
                     FTILLNRKLVDWLIDHIGRVDQKFGAFVNGKS"
     gene            complement(7253..8407)
                     /locus_tag="VFD89_08335"
     CDS             complement(7253..8407)
                     /locus_tag="VFD89_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZJ58228.1"
                     /translation="MFELDRDIRKLEDNIINHRRWFHQNPEIGFEVFGTRDYILEKLD
                     GYGYKEIEILAKTGIKVTIRGKIGKRTLAFRADMDALAMDEKTDLDFESRNKGFMHAC
                     GHDGHMAILLGLAEWLSQNRDDLADNVVLIFQPDEEREGGALPMIEEGLLEDPKVDAI
                     FGLHILPDLPEGKTGTRVGPLMAQTSEVNIELTGKSAHGAMPHKGVDAIVAAAHFITA
                     LQSIISRGIDPTESIIISIGKISGGSTRNILAEKVTMECTIRTFSDEVYNKVRIKILD
                     LLEGVKKTYGVGASYQEGIYYPPVFNDKEWTGKVVDILAPEQYIEAHPLMIAEDFSNY
                     QKVVPGVFLFLGSGNEEKGLTHPLHSNLFDFDERILLHGVQLYKNIITRI"
     gene            complement(8565..8654)
                     /locus_tag="VFD89_08340"
     tRNA            complement(8565..8654)
                     /locus_tag="VFD89_08340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8618..8620),aa:Leu,seq:taa)
     gene            complement(8659..8732)
                     /locus_tag="VFD89_08345"
     tRNA            complement(8659..8732)
                     /locus_tag="VFD89_08345"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8698..8700),aa:Cys,seq:gca)
     gene            complement(8738..8812)
                     /locus_tag="VFD89_08350"
     tRNA            complement(8738..8812)
                     /locus_tag="VFD89_08350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8778..8780),aa:Gly,seq:gcc)
     gene            complement(8820..8895)
                     /locus_tag="VFD89_08355"
     tRNA            complement(8820..8895)
                     /locus_tag="VFD89_08355"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8860..8862),aa:Phe,seq:gaa)
     gene            complement(8911..8987)
                     /locus_tag="VFD89_08360"
     tRNA            complement(8911..8987)
                     /locus_tag="VFD89_08360"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8951..8953),aa:Asp,seq:gtc)
     gene            complement(9004..9078)
                     /locus_tag="VFD89_08365"
     tRNA            complement(9004..9078)
                     /locus_tag="VFD89_08365"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9043..9045),aa:Glu,seq:ttc)
     gene            complement(9083..9158)
                     /locus_tag="VFD89_08370"
     tRNA            complement(9083..9158)
                     /locus_tag="VFD89_08370"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9123..9125),aa:Lys,seq:ttt)
BASE COUNT         2523 a         2109 c         1763 g         2778 t
ORIGIN      
        1 atattatagt ggcaagtgca tgatttgtcc aatattatta aggacaacga tttcatgttg
       61 tccttgttta aaacggagac ttttttgggc gatgtgaaat cccaattata agcggagtat
      121 ccgagaataa tttagattac agatgatagt tggtgaaatt gtggtgacaa caacaataac
      181 agaacactta aaatccatag cgacttattt cgtagcattg atttttgtgg tttcaggtgt
      241 aggtctattt ttattctatt ccgccgaaaa atcggggaag gaattcacga cagaggcaac
      301 tatagccaag tatggggcga tgatctatgt aatttcgggt attggaattg cagtgataaa
      361 gatattcctg caaatacttt cctgagataa cactttaagg ttctatgaga cccaccctcc
      421 ttatttttac ctttatttca aggtctaggt ccgcggtttt aaacagttca ttccattcct
      481 tcccgtgctc tttccaaaat tttgggtaat aaccctctat tttttcatct atttcaaaac
      541 agtccacgcc gtaggtatcc ctgccttttt tataggcggc atccactgta tccctaattt
      601 tattttccac ggctttgccc caaaactcaa ggagtgtttt ggtgtccaat ttcgtggttg
      661 gatttcctac ctcatttata tccccctcta taaaaacctt cgtttctatt ttgatctttc
      721 cgtcctttat ctttggtatc gtacgcacgt ttatattgta ggcattaagg gttagtctgt
      781 cgcccatctc ataggataag tcggcggtta ataggccttt atgaacctct cccaggataa
      841 atcgctgtcc gatgacttcg ctgtcgctaa accagcctac caatttgtaa tccttgaaga
      901 tcccagcgcc tgatacctca actgtatctt tgccggcatt tatcccaacc aaggctacat
      961 cggattctct gataaaattg cgatggaggt atccaatatc agggaatggg aaaataccgg
     1021 aattattagc tgaattttgg gtgactatct ggtctataac cattgctgat gagggtgctg
     1081 tttgaggttc ggaatctaga acctcctttg ctcgcccttt cgcaacgcat actgaggtta
     1141 tcctgcgtat atccggttcc ctaaagaggt aatccagact gctttttatt ccattacgag
     1201 cgtattcctc accaaaaact ataagtttac aatgggatag atttggagta tccccaaaat
     1261 gtttcaatag tgcatctctg gattcggata ggctattgga ctttatactg atctgctggg
     1321 tagatttgct cttttcgcct ttctcccctt taaaggagtc caacagggca acctgatagg
     1381 ttatattaag gctattatcc tcctctaagt ctattccaag ccccaggata aacgcgttct
     1441 tttcaatttc tacccgatcc caacaaccta cggcaaaaat agtagataat gctatcagag
     1501 aggtaagaat cccttttcta accattctca tgctcctttt ttccaaatac caaggcgaat
     1561 atcaaaagcc caaccacgag cattgatatc cctgcgccca aatatccgac gtatgtattg
     1621 accttcctta tctcaggaag atcctggggg agaatcgcta acatatagac tattggcgtt
     1681 aagatataga taaaggtttg atagttcctc aagccaaaga gacgaactgt agacagagaa
     1741 gatatataaa ggaggttggc tacggaagta aacgccgcaa ggatccatag tattacaaag
     1801 aatatatcaa atctatccgc gaaggcgccc ggaaaattta ttattctcgc caacgcgatg
     1861 gtaggataat ttatttgctg ggacggtctt aaaccaaatg cccctacggc cattataact
     1921 agcaggatat agaggacggt gggtatagct atccctatta gtgtgcgggg aatatctgcc
     1981 ttttttcttt caacgaatgg gattattaaa agtatgattt catatcccaa gaaagcggtg
     2041 atcacgttag gcatgccctt gacagttcgc cataatggcg ggaaaagcac gggtcttaac
     2101 cgaacataac taaaactgtt tatacttaag cctatcacca agattagtga aaggattatt
     2161 attggcaaga ataactctga tatctttgct atggcattga taccattaag gctgagatag
     2221 acggcggtaa aaagcataga aaccatgata acttcaaggg gggtgctatg aaggagcaaa
     2281 cccttcaaag catctgaaaa tcccctaaga accagtgatg tagagaacat gaagtatatg
     2341 atgaggataa cccctaaaaa ttttcccaag ggtttcccca acaaggatac taggtattct
     2401 attacagtct ttttttgaaa acgccttcct aaaatattca tggatacagc aaatatggcg
     2461 accaataccc ctgccaagaa tgtagataac agtgccccct cctgggccga ttcagccgca
     2521 attttaggaa gggatataac tatcaccccc aatatagtag tggatataag ggatatgtat
     2581 tgtaaacctc ccaatttatc gtgtttttta accattacca ttctccttat atgggtcttg
     2641 atccattcta tctatatcta cggggtgaac acttattggc cttctccaca gtagattgcg
     2701 gggtagccgc aagatccaat cccttgtatc ttggggaact accggtgctt ggggggtcat
     2761 atagtcaaaa ccaaagctct tcaagcttac caggtgaatg tttagcagaa taaaccccaa
     2821 tgctacccca taaagaccaa gtatagcagc cagaatcatt aagggaaatc taataagcct
     2881 gagagcaata cccgcgctgt aggtgggaat cacgaaggag gcaatggcgg ttatagcgac
     2941 taagagcacc atagaaggac tcgttattcc cgccctaact gcggcatccc caattactat
     3001 accgccgact atgccaatgg tctgacctat agccttggga agcctggcac cggattccct
     3061 taaaagttca atggtgactt ccataaggaa cgcttctaca aaggccggaa agggtatatt
     3121 aaccctattt gccgctatgg ctaatgccag aggagtgggt aagaagctgg ggtgatacgt
     3181 gataacagct atatataatg ccggtacaaa tgcagctaac aatgatgcac tccaacgaag
     3241 aaacctaaga aaagacccaa ttatccatct atcatagtaa tcttcgggag cctgaaagaa
     3301 ttggtataag ccagcgggga cgataatggc aaacgggcta ttatcaacga gtattgctac
     3361 cctgccttct agaaggttgg atacggtggt atcaggcctt tccgtaagct gaacctgggg
     3421 gaacggggaa agggtactgt cctgtatcca ttgctcaatt tggccagttt ccataatttc
     3481 gtcgacatcg acttggttga gccttttctc tacttcctct atgagccccg gatcagttat
     3541 acctttaaca taaacataac tcacatcggt tttactccta cgaccgagtt gaattgtttt
     3601 gatgaccata ttggggtcct tgcattttct tctcaaaagc gcagtattaa cccggagtgt
     3661 ttccgtaaat gcatcctttg gaccccgtac cgacccttcc gtaacgggct ctgacacatc
     3721 cctggccggc catccccttg cattcactat tagaatcttt tcagatcctt ccactatcaa
     3781 ggccacctcg ccggacatta ccatcaacat acacttatcc aaggtgctgg cctctgctat
     3841 ctcaccaaca gctacagcat tttgttttat aaaatcatat gcgctttcaa cctgtatgga
     3901 ttcattcctt gccctttcga gcacggttat ttcatacata agactcttga gtatgttttt
     3961 atttattaca tcggaattaa ccaagccatc aataaaaacc agtgcacagg gaataccctg
     4021 agagcccagc ctgaactccc tgaataccgc atccgaactg tcccccagaa atgctttgaa
     4081 tttttccaaa ttttcttctg tactgttcga tagggtgaca tcaacctttt cttcggcctt
     4141 aagctgcggc gggtcatttt cttccattac ttcacggttt ttagggtttc tatctttttt
     4201 ggtaatcttc atgcatcctc cagtacttta gctacagata aacaatagtc caactaaatt
     4261 tatctatagg tgataaattt tatagctgta gttgtttatc tatatatcaa aaatcctatc
     4321 attaggttac acaaattttg gccatttatt cagtgggata gaaaggaaag acaacaggcc
     4381 atttactaaa cctgttgtct taatgttgac tataatatat ggatggtatc aaattaacga
     4441 aacttgttaa aaaccaatcc cgttataagc ttgggataaa aataagtgga tttttgtggc
     4501 attttttcgt tagctaaaga gacctcttga atttgcctta cggcagtaga cgatataaaa
     4561 aaggtcatct gatggtttcc atgttctacc tcttctatcc cctccataat attatgggtg
     4621 tattctagat tctcctgatt ggctaattcc ttttcaccaa taccgagtat tctgtcaagg
     4681 attagggagt gtagtatgga tacatcgaga tccaagtatg aactatggcg ctttggatgt
     4741 agcgttttca tcatatccat ggatttcaaa gacagggcat aaatgggatg cccactgcct
     4801 acataaagaa cgaaggtatg taagcctttc tcatcaagaa tgttttgaat aatattggac
     4861 ttttccttaa tatcaaaagc cgcaccgtcg tattcttcta tataaaactg gtcccctaag
     4921 ctatctataa gggaatctat actaaaatta cagataccat tgactattct atgtgttggg
     4981 aggataacca atcccgaatc ctccatatct accattgtca tcattacata gttataaagt
     5041 tcatccccgg tgtggttggg atttttatcc ctaagctcat ttctatacgc caacgccgtc
     5101 tcatacctat gatgcccatc ggctatgtat agtttttccc cttttaatgc ctccctgata
     5161 tccccaataa attgaggatc atcaacagcc catactcttt ctttagtttc aatcggccca
     5221 ccatcggcta ggatatcagg cttatgcttt gaacaatagg catcgattaa ggaagggacc
     5281 ctcctcttat gatcgtcata gaggccgaat acctgactaa aatttgcgtt acatgcccgc
     5341 atcagctgca aacggtcagc ttttggctta gacaaggtat tctcatgggg taggattata
     5401 cctttatcaa actcttctaa cctgatcaat ccaatgagac ctgttctaac gcgggatatc
     5461 tgatccccca gtttaaactc ctgttgatat atatataggc aatccttttc ctctgtaagt
     5521 aaaactcctt ggtcctgcca actatttaga atctccccag ctctggtata cttattatca
     5581 tgttcgctgt ctccaggcaa atccttaggg agttccagcc taatagaatt gtagggattt
     5641 acattataaa atccctcttg ctcttcgctg ctaataatat cataaggggg agtaaccaca
     5701 tcggaaacat cacccacaat atcgacatta tatcgtagag ccttaaatgg tatgatttct
     5761 gccattttat ttgacgcccc tttcatcatg tattttgcta cattatcttc acctacacaa
     5821 tcttatatga attaagggga atcgtcaata tccatggaat tatgtggatg atcattgtaa
     5881 ttcctttaaa ttatttagat aatctattaa atccgaggcc tctcttttag ttagtgcttt
     5941 gctgtccacc ttctgatacc tactggagat taaatccttc atctcatctt cccctagatt
     6001 gagggaagta tatatggcaa atatagccct gcgttgaggg tcggtaatgt tatcatcctg
     6061 ttcctggaag tcgctatgat tagatccggc tacagggcta tcggcaatcc tctcaccctc
     6121 ctccagatca ggtgcaaatt gggtaccata acccaaagcc gcaagggcac gtccataggc
     6181 cttggtatag gccttttcta tataatcccg aaaatcctcc ccggtctcgg atcctattcc
     6241 tacttcaaca ttacctttgg tatcagttac ctggagttca aaataggcga aacgattgtc
     6301 gatatcgatt attttatcta acacaattgt ttgggtgttt tcgaaattat tttcacgaaa
     6361 ccaaaccatc cgccatttta tctctaaata atctgcaaat tgggtctccc atgaattggt
     6421 tttagggttc ctcctgcgca atgggagctt tgttaagtat tcttccgggt taaacttttg
     6481 tggtggcatc tctcaagctc cttataccat gtattcggtg gttaccgtac acatagtatg
     6541 tgtcaaatca agaaaaattc actaaagata ttttggtaat taggacttcc catttacaaa
     6601 ggcaccaaat ttttgatcca cccttccgat atggtcaatg agccaatcga ctaacttcct
     6661 attcagcaga atagtaaact ggatgctgac cccttcctct tcgaagcgtc tgtacaatct
     6721 ttccacttca tgggtaaagg tattatggag cctagcatgt gcatccctat ctggataatt
     6781 gtgaatcctg tgttgttcct cttcatattg aaaatgttcc ctaacatacc tagaaaggaa
     6841 attcaaggca ttggcgactt cctgtttgct accccctttt ctgcaggcct ccagcaaacg
     6901 ggatacttgt tccaccaagg ccttgtgttg attatcaatt tgtccaacct caatattcca
     6961 actattttgc catttgattg acatatattt acccccccct gatcgctaca ataggatatt
     7021 ttaaatggat agtgttatta cgcctgttta tctcttcaga gaagatgttt atgtagtcta
     7081 tttccagatg tataaccggg aacccgtaag gtataaataa ccgagatcaa cagagatgaa
     7141 acatatacca ataattttcg attcaatggg ctacaatacg acatagcaac aacataggtg
     7201 atattaatta tcacgctatt aaaattgcca aggttgctat tcttctaagt ttttatatcc
     7261 gggttattat atttttatat agctgaacac cgtggagtaa gattctttca tcaaaatcaa
     7321 ataaattact gtggagggga tgggtaagcc ctttttcttc atttccgcta cccagaaaaa
     7381 gaaacacgcc ggggaccacc ttttgatagt tagaaaagtc ctctgctatc atcaacgggt
     7441 gcgcttctat gtattgttcc ggggctagta tgtctacaac cttcccggtc cattccttat
     7501 cattaaaaac aggtggataa taaatccctt cttggtaact ggctcctaca ccataggttt
     7561 tttttacacc ctctagcaga tcaaggattt ttatccttac tttattatag acttcgtcac
     7621 taaaagtcct gatggtgcat tccatcgtta ccttctctgc taggatattc ctggtactac
     7681 cgccgcttat cttgccaata gaaatgatga tgctttccgt gggatctatg ccccgtgaaa
     7741 ttatggactg aagagccgtt atgaaatggg ccgccgctac aatggcatct acccctttgt
     7801 gcggcatagc tccgtgggca ctttttcccg tcagctctat attcacctcg ctggtttggg
     7861 ccatgagtgg accaaccctt gttccagttt taccctccgg caaatccggc aaaatatgaa
     7921 gtccaaaaat ggcatcgacc ttgggatcct ctagtaaacc ctcttctatc atgggtaggg
     7981 ccccaccctc cctttcctca tcgggttgga atatcaatac aacattatct gcaagatcgt
     8041 ctctattttg ggaaagccat tctgccagac ccaatagaat ggccatatgc ccatcatgtc
     8101 cgcaggcatg cataaaaccc ttgttcctcg attcaaagtc taaatcggtc ttctcatcca
     8161 tagccaaggc atccatatct gccctaaaag ctaaagtcct tttcccgatc tttcctctaa
     8221 tggtgacctt gatacccgtt ttggctaaaa tctcaatttc tttatagcca tacccatcta
     8281 gtttctctaa aatataatcc ctagtcccaa atacttcaaa tccgatttcg ggattttgat
     8341 ggaaccaccg tctatggttg atgatattat cttctaattt tcggatatcc ctatccagtt
     8401 caaacattct cccaccgcct cattttttat atagctctcc cttgatggtc ttttatcagc
     8461 ctagttaaat aatatcattg atcggataat gtttgcatat gtttgcatat aatcgggaga
     8521 taactaaaaa agagcttgag taaaattctc aaaccccttg gtattggtgc cggagaccgg
     8581 aatcgaaccg gtacgatctt tgttggatcg caggatttta agtcctgtgc gtctgcctgt
     8641 tccgccactc cggcatattg gaggcggcac ccagatttga actgggggta aaggatttgc
     8701 agtcctctgc cttaccactt ggctatgccg cctaatatgg agcggaagac gggattcgaa
     8761 cccgcgacat tcgccttggc aaggcgacgc tctaccactg agccactccc gcttatctat
     8821 ggtgcctcgg ggcagaatcg aaccaccgac acgcagattt tcagtctact gctctaccga
     8881 ctgagctacc gaggcaattt atttatataa tggcgacccg gaaggggctc gaaccctcga
     8941 cctccagcgt gacaggctgg cattctaacc aactgaacta ccgggccatc taacaaaaaa
     9001 cattggtgac ccctagggga ctcgaacccc tgataccgcc gtgaaagggc ggtgtcttaa
     9061 ccacttgacc aaggggccac tatggtgacc catcggcgat tcgaacgccg ggccacttga
     9121 ttaaaagtca agtgctctgc cagctgagct aatgggtcaa gtcttaagcg aca
//
LOCUS       DASRNX010000212         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_419013,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000212 DASRNX010000000
VERSION     DASRNX010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419013"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2285
                     /locus_tag="VFD89_08375"
     CDS             <1..2285
                     /locus_tag="VFD89_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HZJ58229.1"
                     /translation="PYIALGAATIFVGLVKLRRRGLYLPLVLLIFALGGVLNTYIHSI
                     PNTDLDDFIGQAPVTHGQVLEQVKQRDSSASFILEARGIAHNNVQHGINCKIRMTVYF
                     SSDNGEDLGIDIGDWIEVRGKIERPQGRRNPKGFDYRSYLGRRGIYYTMGVPRGNILS
                     IKAGRLPWPNSWLRSARDYMARVFDTYVGGGESGLIKAMLIGEKWALPYRVTEEFRNT
                     GIAHILAISGLHIGFIILLLSWLTGILKLSPKAAFFVQGIVLGVYCILVGGSPSVLRA
                     SIMGTIILGGRVIGRKAEPLNSLFLAAFIVLLINPLDLKEIGFQLSFGAAAGIILYSR
                     PIGTRLGLLPKAIADSLAVMLSAQMGTWPLLAYYFNAFSPIGFVANLILVPIAGMIVI
                     LGPILLVVAALFPALAIFLGRGMWAISHALIGTNAWLSGFSWSAFRVVSPSIISLFIY
                     YAILLIISQERPDFIKRPAITCAVLAFVALVPIVMGPVFDKDLRIVFVDVGQGDCIYI
                     KSPDGKHILVDGGGISPEAGDFDVGTEIVVPFLLKNGIKRLDAVIMSHAHDDHIGGLI
                     PVIEDLKVDAFMEYPPGEQSAQYQTLSDLVAKKGIRRIEARGGETYRIGKEMFLDIIY
                     PVTDTKVLNRLYERNENNLSLVVRIRCQDIGILLTGDMEGGVESYLSKVWDESVSLLK
                     VAHHGSKTSSTEGWIDIIDPDIAVIQSGKNNFGHPDPAVIQRFEDRGSQVFRNDREGA
                     ITLIYRKGKWLVTADNHYAN"
BASE COUNT          651 a          468 c          621 g          675 t
ORIGIN      
        1 atccatacat agcccttggg gcagcaacta tatttgtggg gcttgtaaaa ttaagacgca
       61 ggggtttata tctgcccctg gtcctgctca tatttgcttt agggggtgtc ctcaacacat
      121 atatccacag cattcccaat acggatttgg atgattttat tggacaagcc cccgtaaccc
      181 atggacaggt tctggaacag gtaaaacaaa gggattctag tgcgtccttt atcttggagg
      241 cccggggtat agcccataat aatgttcaac acgggatcaa ttgtaaaata aggatgacgg
      301 tctatttctc ctcggataat ggggaagatc tcgggataga tatcggtgat tggatagagg
      361 taaggggaaa aatagaaaga ccccaaggcc gtagaaatcc caaaggtttt gattataggt
      421 cctatctcgg acgcaggggg atctactata ccatgggggt tcccagggga aatattttat
      481 ctataaaggc tgggagactc ccatggccaa attcttggtt aagatctgct agggattata
      541 tggctcgggt ctttgataca tatgtagggg gcggagaatc cggactgata aaggcaatgc
      601 tcatagggga aaaatgggca ttgccatata gggttacaga ggagtttagg aacactggaa
      661 ttgcacatat tctggccata tcaggactgc atataggatt tatcattcta ttattatctt
      721 ggcttaccgg aatcttaaag ttatcaccca aggctgcctt ttttgttcag gggatagtcc
      781 ttggggttta ttgtattttg gtgggtggga gcccgtcggt gctcagggct tcaatcatgg
      841 ggacaataat cctgggcgga agggtgattg ggagaaaggc tgagccctta aacagcttgt
      901 tcttggcagc ttttattgtc cttctcatta atccgctgga cttaaaggag ataggatttc
      961 agctttcctt cggtgcggcg gcaggtatca tattatattc aagacccata ggcaccaggt
     1021 tagggctgtt gcccaaggcg atagcagatt ccctggcggt tatgctgtcg gcgcagatgg
     1081 gtacatggcc cctcctggca tactatttta atgcattttc gcccataggc tttgtagcta
     1141 acctcatctt ggtccccata gcgggtatga tcgttatcct tggaccgata ctattggtag
     1201 ttgcagcgct gtttcccgcc ctggccatat ttttaggccg tgggatgtgg gccataagcc
     1261 acgctctcat aggcaccaac gcatggctat caggcttttc gtggtctgcc tttagggtcg
     1321 tatctccgag cattatatcc ctttttatct actacgctat actcttaata atatcccagg
     1381 aacgccctga ctttattaaa agacctgcaa ttacctgtgc agtattagcc ttcgtcgcat
     1441 tggtacccat agtcatgggg ccggtttttg acaaggatct taggatcgtc tttgtggatg
     1501 tgggacaggg agattgtata tatataaaat ccccggatgg aaagcatata ttggtagatg
     1561 gtggcgggat atccccggag gcgggtgatt ttgatgtagg cactgaaata gttgttccct
     1621 ttctgctgaa aaatggcatc aagaggcttg acgctgtgat catgtcccat gcccacgatg
     1681 atcatatagg agggttgata ccggttatag aggatcttaa ggtagatgct tttatggaat
     1741 atcccccggg ggaacaatca gcacaatatc agaccctaag tgatctagtg gccaaaaagg
     1801 gtattaggcg catagaagcc aggggcgggg aaacctatag aataggcaaa gagatgtttt
     1861 tagatattat atatcctgtc acagatacaa aagtattaaa taggctatat gaaaggaatg
     1921 aaaataatct atccttggtg gtcagaatac gctgtcagga catcggaatt ttgcttaccg
     1981 gagatatgga ggggggcgtc gaatcctatc tatcgaaggt atgggatgaa agcgtttcct
     2041 tgttgaaggt ggcccatcat gggagcaaga cctcatccac agaaggatgg atcgatataa
     2101 tagatcctga tatagcggtg atccaatccg gaaaaaacaa tttcgggcat cccgatcccg
     2161 ccgtaataca aaggttcgaa gaccggggca gccaagtgtt tagaaatgat agggaagggg
     2221 caattaccct tatatacaga aaaggcaaat ggctagtcac tgcagataat cattacgcaa
     2281 actagattta tcggcacgat aattgcaacg gttttatgca atttcatggt gataaatttt
     2341 tagttggagt tgtttgtctg tcccatagca aagaatagaa aatcgaaaaa tgtcgtggca
     2401 tatattatat tgtta
//
LOCUS       DASRNX010000213         7540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_257491,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000213 DASRNX010000000
VERSION     DASRNX010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257491"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /gene="dnaG"
                     /locus_tag="VFD89_08380"
     CDS             complement(<1..1263)
                     /gene="dnaG"
                     /locus_tag="VFD89_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZJ58230.1"
                     /translation="MSSYLPEDFIEEVRIANEITDVLSEYLALKPKGKNYFGLCPFHN
                     EKTPSFSVDPERQFYHCFGCGVGGNVFNFVMAQEGLDFIDSVKFLAQRKGISLPGYAE
                     NPQDEETRKHNERLHELNREAALYYHDRLLSHLGKRALEYLKSRGIDQRIIKTFGLGY
                     APNSWDSTKNYLLGKGYDTQLLTEVGLVLEKNQSVYDRFRDRIMFPIIDHRDRVVGFG
                     GRVLDDSSPKYLNSPESPIFNKGGVLFGLNLARKKRPIANLVIVEGYLDVISLYRFGI
                     ENGVASLGTALTPEQARIMRRYTQNIYTAYDGDAAGQRATVRGLDILKSVGCNVKVIV
                     FPKGMDPDDILVDHGVEYFNKLLDNAVSLTDFKIDQLKAQYDLDNQDDKVKFTTKAVS
                     ILSDVDNLLERDIYIQRLNSLTGFRPELL"
     gene            complement(1503..2486)
                     /locus_tag="VFD89_08385"
     CDS             complement(1503..2486)
                     /locus_tag="VFD89_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HZJ58231.1"
                     /translation="MIIRKETERWEQDNFSPYAALSAQSKGRLFPQEKCDIRTDFQRD
                     RDRILHCKSFRRLKHKTQVFIAPEGDHYRTRLTHTLEVSQISRTIARALKLNEDLTEA
                     IALGHDLGHTPFGHMGERTLSSLSKNGFKHNLQSLRVVEVLENGCGLNLTWEVRDGIK
                     NHTESEIPSTLEGKVVRLSDRIAYINHDIDDAIRANVISQDSLPRPCILILGETHGQR
                     INKMVEDVIFNSLNKAHIRMSDDVENATNELRNFMFEKVYIGTVSKSQENEADYVLKR
                     LFYYFSDNPHMLPTEYFKNISQWGTDQVVCDYIAGMTDRYALGVFETLQDP"
     gene            complement(2726..3433)
                     /locus_tag="VFD89_08390"
     CDS             complement(2726..3433)
                     /locus_tag="VFD89_08390"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZJ58232.1"
                     /translation="MILYVIRHGDPDYTTDTLTPKGKLQADALAKRLGVYGVDVIYSS
                     PLGRAKETAKATCDLLDMECNIEPWTSESEAWERFAGIMPDGNRGWVFSQQTTILKND
                     ETVNLGEAWHEAEGLQMIDGKKGYDELIDSSDKFLYKLGYERKGSVYKIIRPNEDKVA
                     LFCHQGFGLTWISHLLQIPPHLFWSSFDMSHSSFTVFEFKNNSDGLTTPKCLSLSDAS
                     HIYKEGLPLKYNNVIEF"
     gene            complement(3691..5628)
                     /locus_tag="VFD89_08395"
     CDS             complement(3691..5628)
                     /locus_tag="VFD89_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZJ58233.1"
                     /translation="MSQMFPHLFSEGSIGNLKIKNRVLKAPQSSGMSNIDGSVSDRLI
                     RYYTEQARGGTGLVVVEYAYVDDIASKSAHCHLGISSNEHIPGLAWLAETIMDNGARA
                     GIQIEHCGRQKFLGTPPIAAPSAMPWPALYDRVGLDAVPHVLSISEIQDIVEAFGDAA
                     QRAVSAGFELVEIHGAHGYLLTNFFSPHTNKRTDWYGGSLENRMRIYIEITRNIRKKI
                     GPDFPLTIRLSGTDYEPDGFPIEETIVLSKVLRDEGIDAIHVSGGDHHTMIHQVSPMS
                     MSICHNTWAAEAIKKEVDIPIIASGSITLPEYAEDIISKGKADFVGLGRPLWADPYWP
                     KKAMEDRPEDIRPCIRCNEGCLERTFFRFKAVTCAVNPTISREGQLEITPAEEIKRIA
                     VVGGGPAGMEIARVAKLRGHDVTLFEKNRLGGALNEAAVPEFKSDIRPLTKSLITQVE
                     KLGIPVVEKEAHVEDLTGFDAVVVAVGGVEIKLNLPGEDKEHVVSALDILNNHREVGD
                     KTVVIGGGLVGAETALYLAEGGKKVTVVEMQDAIMKDVATTDQLAYGERIAKTQMEVL
                     IEHRLVEIKDKSVVVEHGGRLKEIEADTVIMAVGFRPQSQLEEGLKSIGGPEVHSIGD
                     CVEPAKIFDAFHAGYKLGLRI"
     gene            6049..6714
                     /locus_tag="VFD89_08400"
     CDS             6049..6714
                     /locus_tag="VFD89_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZJ58234.1"
                     /translation="MLYSTNFWLRGDLKAFKNLFKNFAISTQKFDPNTIIRCPGYKAD
                     NLYYLIEGLVKASMTNSNGLEKTIGYHMPGTLFGLDSLVENEDTLVTFTPITDTLCYE
                     LSNVSIKNMILEDKMFAIKIAEYYCKVLRLMCLETANQSFNNATARVSNFLYMYYLST
                     RDNGYNHLYLTQEEVSSIVNVSRVQVARIYRLLREKDIIETGRGQVTITNPGNLYSFC
                     HFS"
     gene            complement(6762..7538)
                     /locus_tag="VFD89_08405"
     CDS             complement(6762..7538)
                     /locus_tag="VFD89_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HZJ58235.1"
                     /translation="MRLYIKYISIHFKSQMQYKASFFLTIIGQFLMSFTVLLGLYFMM
                     DRFKTVGGFTLEQVFLCFAVVLMAFSLAECFARGFDLFPQMLSNGEFDRVLVRPRGII
                     FQVLAGKVEFSRLGRLMQATMVFSYAIPKSCVVWTWDKILTLLLMVVCGMLIFFGLFL
                     IYASFTFFTIEGLEFMNVLTDGGREFGTYPYSIYGDGILKFVTYIIPLALFQYYPLLY
                     LLDIKKDIFYMLTPLLGLLFLVPCYLFFLFGLSRYKSTGS"
BASE COUNT         2073 a         1810 c         1405 g         2252 t
ORIGIN      
        1 taggagttcc ggtctgaatc ctgttaaact atttaatctc tggatgtata tatccctttc
       61 cagaagatta tcaacatccg ataatatact aacggccttt gtggtgaact ttaccttatc
      121 atcctggtta tccagatcat attgggcttt cagttggtct atcttaaaat cggttagaga
      181 tacggcattg tccaaaagtt tgttaaaata ttcaacgccg tgatctacca gaatatcatc
      241 agggtccatg ccctttggaa atacaataac cttcacgttg catccgacac tttttaatat
      301 atccaggccc ctaactgtag ccctttgacc cgcagcgtcc ccatcatatg ccgtgtagat
      361 attttgtgta tacctcctca ttattctggc ctgctccggc gtaagggcag ttcctaatga
      421 ggccacacca ttttcaatac cgaacctata aagggaaata acatccaaat atccttcgac
      481 tattactaaa ttggcaatag gccgcttttt tcttgctaag ttaaggccaa ataaaactcc
      541 gcctttattg aaaatggggc tttccgggga gtttaaatac ttgggagagg aatcatctaa
      601 aacccttccc ccaaatccca ctaccctatc ccgatggtca attatgggga acatgatcct
      661 atcccgaaat cgatcgtata cactttgatt cttttcaagg accaagccta cctctgtcaa
      721 tagctgtgta tcatatcctt tgcccagcaa atagttctta gtggagtccc aagaatttgg
      781 ggcgtatcct aagccaaatg tttttattat tctttgatct attcccctgg actttaagta
      841 ttccagagct cgcttaccaa ggtgggacaa gagtctgtca tggtagtata atgctgcctc
      901 cctatttagt tcatgaagtc tctcattatg ctttctggtt tcctcatcct gtggattctc
      961 tgcgtaaccc ggcagagaaa tccccttacg ctgagccaaa aattttacag aatctataaa
     1021 atccagcccc tcctgggcca taacaaaatt aaaaacattg ccacccacac cgcaaccaaa
     1081 acaatggtag aattgccttt cgggatccac ggaaaaggag ggcgtcttct cattgtgaaa
     1141 cgggcacaaa ccaaaatagt ttttaccctt gggcttaagc gccaaatatt cggataatac
     1201 atccgttatc tcgttggcta tccttacttc ctctataaaa tcttcgggca aatagctgga
     1261 cattgtcacc acctgtcatt ctcacaatat atatcaacac agcatttcaa aataagcctc
     1321 gatgttctaa agcatcataa agtttgctaa caaaactcct aggtttatat tcgacatgct
     1381 ttgccaattt ccttctttta atcacaacat tttgataagg ttatcggggg atccccccca
     1441 acatgctgtc tctacttatt atgttagtta ttcccaagat ttatgattat ttttattatt
     1501 ccctaagggt cttgtaatgt ttcaaaaact cccaaggcgt atctatctgt cattcccgct
     1561 atataatcgc ataccacttg atctgtcccc cattgggata tattcttaaa gtattctgtg
     1621 ggtagcatat ggggattatc tgagaaataa taaaagagcc tctttaaaac ataatcggct
     1681 tcattttctt ggctttttga taccgttcct atatatacct tctcgaacat aaaatttctt
     1741 aattcatttg tggcattttc cacatcgtct gacatcctta tatgggcttt attcagactg
     1801 ttaaagatga catcctccac cattttattg atgcgctgtc catgggtttc acccaatata
     1861 agaatacagg gccttggtaa actatcctgt gaaataacgt tggcccttat agcatcgtct
     1921 atgtcgtgat ttatataggc aattcgatca cttagtctta caacttttcc ctccaaagtt
     1981 gaaggtattt cggattcggt atgattcttt attccgtccc taacctccca agtaaggttg
     2041 agaccgcatc cattttccaa tacttccact accctaaggc tttgtaagtt atgcttaaat
     2101 ccatttttgg acaagctgct caaggttctt tcgcccatat ggccaaaggg cgtatgaccg
     2161 aggtcgtgac ctaaggctat tgcctctgtg agatcctcgt tgagctttag agcccttgct
     2221 atagtgcggc taatctggga gacctccaaa gtgtgggtaa gccgggttct ataatgatct
     2281 ccctcggggg caataaatac ctgagtcttg tgctttaagc gccgaaagga cttgcaatgg
     2341 agtatcctat ccctgtccct ttgaaagtcg gtgcgaatat cacatttctc ttggggaaat
     2401 aatcggcctt tggattgggc gctcaaggcc gcatagggtg agaaattgtc ctgttcccat
     2461 ctttccgtct ctttgcgaat aatcatatta tcccctttaa acccattcct tttgctttac
     2521 tcatctatat tatatataca acatatatgt ccaatatcct tctatttccc caagaaaatt
     2581 gttccctcaa gggatgcccc ttgatatatt agcctttact cgccacggac atcgacccga
     2641 taccccggcc taatttattt ccccatatgg gggtatgtct tttctcgtgt agttataaaa
     2701 ataggattcc acaataacat tagaactaaa actctatcac attattgtat tttaggggca
     2761 ggccttcctt ataaatgtgg gaagcgtcgg acaaggacag acatttaggt gttgtaaggc
     2821 cgtcactgtt gttcttaaat tcgaatacgg taaagcttga atgggacata tcaaagctcg
     2881 accaaaatag gtggggcggt atctgtaata aatgggatat ccaggtcaaa ccaaacccct
     2941 gatgacaaaa caaggctact ttatcctcat ttggtcttat aatcttgtat acactgccct
     3001 tacgttcata gcccagtttg tataaaaact tatccgaaga atcaattaat tcatcatagc
     3061 cctttttgcc gtcgatcatc tgcaaacctt cggcttcgtg ccatgcttcc cctagattaa
     3121 cggtttcatc attcttcaaa atcgtagtct gttggctaaa aacccagccc ctgtttccgt
     3181 ccggcattat accggcgaat ctttcccaag cctccgattc gctggtccat ggttcaatat
     3241 tgcattccat gtccaacaga tcgcaggttg cctttgctgt ctccttggct cttcccaaag
     3301 gtgacgaata aattacatcg actccatata cccccagcct ttttgccagt gcatccgctt
     3361 gtagttttcc ttttggagtt agggtatccg tggtataatc cggatcacca tgtctaataa
     3421 catataaaat catgcagaat tgcctcctta tgtgattgct gcaaaaagta aatacccaaa
     3481 actgccgcta aatatcagtc aaaggtccaa aattttgcat acagcaacaa ttgtacatta
     3541 tttttgactg tttggcaaaa acgaaaggtc cgggacacaa ataaaaagcc gattaaaggt
     3601 ttatcaccct taatcggcaa ttataataat ttaattcctt gtgccaatta tctatatatc
     3661 ggtaatgagc tattgaacgc ccagtaccat ctaaatcctt aaccccagtt tatatcctgc
     3721 atggaaagca tcgaagatct tggcgggctc tacgcaatca ccgattgaat gcacttccgg
     3781 ccccccaatt gattttagtc cctcttccag ctgggactgg ggtctaaagc ctacggccat
     3841 tataacggta tcggcctcaa tctcctttag tctgccccca tgctccacaa ctacactctt
     3901 atcctttatc tctactaatc tatgttcgat tagaacctcc atttgggtct tggctattct
     3961 ctctccatag gccagttgat ctgttgtggc cacatccttc attatagcat cttgcatctc
     4021 cacaacggtt acctttttcc cgccctcagc caaatagagc gcagtctctg cacctaccaa
     4081 tccaccgcct atcaccactg tcttgtctcc gacttctcta tggttattga gaatatccaa
     4141 ggccgagact acatgttcct tatcttctcc gggtaaattt agcttaatct cgacgccccc
     4201 gacggctact actactgcat caaagccagt aaggtcttca acatgggctt ctttttcgac
     4261 aacgggaatc cccagcttct ccacctgggt tatcaggctc ttggttaggg gccttatatc
     4321 cgacttaaat tccggtactg ctgcttcgtt aagggcaccg cccaacctat tcttttcaaa
     4381 taaagtaaca tcatggcccc gaagctttgc caccctggct atttccatac cggcaggtcc
     4441 gcctcctacc acggcaatcc tcttaatctc ctcggcaggg gttatctcca gctgtccttc
     4501 tctggatata gtgggattca ctgcgcaagt aacggcctta aatcggaaga aggtcctctc
     4561 cagacatcct tcgttacatc tgatacaggg acggatatcc tcgggcctat cctccattgc
     4621 ttttttcggc caataggggt ctgcccataa gggtcttcca agacctacga aatcggcctt
     4681 gccctttgag ataatatcct ctgcatattc aggcaacgtt atggaaccgg aggcaataat
     4741 aggaatatct acttcctttt taattgcctc ggctgcccag gtattatggc agattgacat
     4801 ggacatgggg cttacctggt ggatcattgt atggtgatcc ccgccggata cgtgaatggc
     4861 atcgatacct tcgtccctta ataccttaga taaaacaatg gtctcttcga tgggaaaacc
     4921 gtccggctcg tagtccgtac cgctgaggcg aatcgtcaac ggaaagtccg ggcctatttt
     4981 ttttcggatg tttctggtga tctctatgta tatgcgcata cgattttcca gggaacctcc
     5041 gtaccaatca gttcgtttgt tggtgtgggg tgagaagaag ttagtcaata aatatccatg
     5101 ggccccgtgg atctcaacca gttcaaaacc ggcggataca gcccgctgtg cagcatctcc
     5161 aaaggcttct actatatcct gtatttcgga tatactcagg acatggggta cagcatccaa
     5221 tccaacccta tcatagagtg ccggccatgg catagcggag ggtgcagcaa tggggggtgt
     5281 acctaaaaac ttttgtcttc cgcaatgttc aatctggatt ccggcccttg ccccattatc
     5341 catgatggtt tctgcaagcc atgccagtcc gggaatgtgt tcattgctgg atatgccaag
     5401 atggcaatgg gccgatttgc tggctatatc gtcaacataa gcatactcaa ctacaacaag
     5461 ccctgtcccg ccccttgcct gttcggtata atacctgatc aatcggtcgc tgacggatcc
     5521 gtctatattg ctcattccgg aggattgggg tgcctttagc acacggtttt tgattttcag
     5581 attacctata cttccttctg agaatagatg aggaaacatt tgtgacattt ttctacctcc
     5641 aaacacttta ttttgttagt ttatacattc actgctaatt gtacaggata tccaattaaa
     5701 acactgtcat ccttgttaca taattaacat actttttgaa caggtattaa tccatatgaa
     5761 tcaacatatc ataaagcttg agggtacata tgggtattcc aatgtatcat agtatacatt
     5821 acgttcgaac aactgcctac ctgcaagcct ttagccaggg cggatgtgac atacaaaaga
     5881 tctatccagt ggtctacaat tatacatacg tcggaatcta taataacacc gttaatagtt
     5941 taacatttgt taggtctcca ctttatccct tcaatacatt gactgtgtcc cgactttgac
     6001 ttatgataag aatatacaga tatttggaaa tttatagggg gggatcatat gctctattca
     6061 acaaactttt ggcttcgggg tgatttaaag gcctttaaaa acctcttcaa aaactttgct
     6121 atttctaccc aaaagtttga cccaaatact attattcgtt gccccggcta caaagccgat
     6181 aatctctatt atcttataga aggtctggta aaagcctcca tgaccaacag taacggcctg
     6241 gaaaaaacta taggttatca tatgccgggg accctcttcg gtctggactc cctggttgaa
     6301 aacgaggata cccttgtgac ctttacacca ataacagata cgctctgtta cgagctttca
     6361 aatgtttcaa taaaaaatat gatcctggaa gataagatgt ttgctattaa gatagcagaa
     6421 tactattgta aagtactgcg gctgatgtgc ttggagacgg ctaatcaatc ctttaacaat
     6481 gcgacggcca gggtctctaa ctttctatat atgtattatt tgagtacccg tgataacggg
     6541 tataaccacc tttacctaac ccaagaggag gtatcctcca ttgtcaatgt atccagagta
     6601 caagtcgctc gcatctacag gctccttcgt gaaaaggata taattgagac cggtcgcggc
     6661 caagtcacca tcaccaatcc cgggaatctc tactcctttt gccacttctc ttagtcaaat
     6721 tcccttttgc gtaacatata tagttataaa tgaacctaag actatgatcc ggtagattta
     6781 taccggctta agccaaacag aaaaaacaaa tagcagggaa ccagaaacaa taacccaagt
     6841 aagggtgtaa gcatgtaaaa aatgtccttt tttatatcaa gaaggtataa taaggggtaa
     6901 tactgaaata atgccagcgg aattatatag gtgacaaatt ttaagatacc atcaccatat
     6961 atggagtaag ggtatgtgcc aaattctcgt cccccatcgg tcaatacgtt cataaattct
     7021 aacccctcta tggtgaagaa ggtaaatgat gcatagatga ggaacaatcc aaagaatatg
     7081 agcatcccgc atacaaccat gagtaacagc gtcaatatct tatcccaggt ccaaactaca
     7141 cagcttttag gaattgcata ggaaaaaacc atggttgcct gcataagtct ccccaaccgc
     7201 gaaaactcaa cctttcctgc taatacctga aatatgattc cccggggcct gaccagcacc
     7261 ctatcaaatt caccgtttga aagcatctga ggaaacagat caaagcccct ggcgaagcat
     7321 tcggccaggg aaaatgccat aagcacaacc gcaaagcata ggaacacctg ctccagggta
     7381 aagcccccaa cggttttaaa tctgtccatc ataaagtaca accctaaaag aacggtgaag
     7441 gacataagga attgcccaat gattgtcaga aaaaatgatg ccttatattg catttggctt
     7501 ttaaagtgta tagagatata ttttatatac agtctcatat
//
LOCUS       DASRNX010000214         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_419267,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000214 DASRNX010000000
VERSION     DASRNX010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419267"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VFD89_08410"
     CDS             complement(<1..1098)
                     /locus_tag="VFD89_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HZJ58236.1"
                     /translation="MARGNFILTYDIGTTGNKCTVFDEEGKTVWSTVSGYDTIYPQPG
                     WSEQRPEDFWKSVIEGTEELIEKYGMNPMDIEVIGLSGHMNGCLPVDSQGQPLFNNII
                     HSDVRSEPECQTLLEIFQSRYIYEITGNRIDPHYTLPKILWLKKHYPDIYNDTAYFLN
                     TKDYIAYKLTGKLGFTDYSDASLTCILDIKAKKWSEDIAGGVGIDMGKLPHIMKSHDI
                     LGSLSKEAALILGLKEGIPVVVGGGDGACAAKGAGVVREGMAYNYIGSSSWISTPSKN
                     PILDKEARIFNICDLDGIHYNVIGTVQSATISYDWVVDNLGRHEKELAAKNNDSIFDI
                     IQGLAKNSPAGSRGVFFVPYLMGERTPHWDAD"
     gene            1565..>2652
                     /locus_tag="VFD89_08415"
     CDS             1565..>2652
                     /locus_tag="VFD89_08415"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HZJ58237.1"
                     /translation="MKYIVVLGDGMADRPIPELNNKTPLQCAKKPNMDSLASRGEMGL
                     VNTIPEGMPPGSDVANLSTMGFDPRLYYTGRSPLEAVSMGIELADTDVTLRCNLVTLS
                     EDKAYEDKTMLDYSSDEISSEEAKILIDDIGAQLNGPDISFYSGISYRHCMVWSGGPV
                     DLNLTPPHDILEKPIGDFLPKGNTNKIILEMMEKSHEILKNHPVNLARISKGLGPANS
                     IWLWGEGKKPSLPSFQETYGKKGSVISAVDLVKGIGISAGLRSVHVEGATGNIDTNFV
                     GKAEAALEELKSGQDFVYIHIEAPDECGHRREIENKVKSIELIDRQVLGTLLEGLEQY
                     EDYRIMILPDHPTPLALRTHTSDPVPYI"
BASE COUNT          710 a          627 c          587 g          728 t
ORIGIN      
        1 atcggcatcc caatgggggg tccgctcccc catgagatag gggacaaaga atacgcccct
       61 ggaaccggca ggcgaattct tggctaagcc ttggattata tcgaatatgc tgtcattatt
      121 tttagcggcc aattccttct catgcctgcc taaattatcg actacccaat cataggagat
      181 ggtggcactt tggaccgtac ctattacgtt atagtgtatc ccgtccaagt cacagatgtt
      241 aaaaatccta gcctccttat ccaaaatggg gttcttgctg ggggtagata tccaagagct
      301 gcttcctata tagttatagg ccatgccctc ccttactacc ccggcgcctt tggcagcgca
      361 tgcaccatcc ccgccaccaa ctaccaccgg tatgccctcc ttaaggccca ggattagggc
      421 ggcttcttta ctaagactcc ctaatatatc atgggacttc ataatatgtg gtagcttgcc
      481 catatctatc cctacgcccc ctgctatatc ctcggaccat ttcttggcct taatatctaa
      541 aatacaggtt agagaggcat cggaataatc cgtaaagccc aatttccccg taagcttata
      601 ggctatataa tccttggtgt ttagaaaata ggccgtgtca ttatagatgt cggggtaatg
      661 cttcttaagc cacagtatct tcggtagggt atagtgtgga tcgattctgt tacccgtaat
      721 ttcatatata taacggcttt gaaatatctc taaaagggtc tgacactccg gctcgcttcg
      781 gacatcggag tgaattatat tgttaaaaag gggttggcct tggctatcta cgggcagaca
      841 accgttcata tgtccgctaa ggcctatgac ctctatatcc atgggattca tgccgtattt
      901 ttcaatgagt tcctcagtac cttctataac actcttccaa aaatcctcgg gccgctgttc
      961 ggaccacccg ggttgggggt agatggtatc ataccccgaa actgtagacc acaccgtctt
     1021 gccctcttcg tcaaataccg tgcatttatt acccgtggta cctatatcgt atgttaaaat
     1081 gaaatttccc ctggccattt ttttacctgc ccttcataat tacttgtata atgctatttt
     1141 aatccatatc cagtttaaaa tgaagggtga cttgattaat atatctctat aaccaataat
     1201 gcctatggac aggcccgagg gttttgatat aaaatttgat gtagagtttc aaattcaatc
     1261 tggtgtccta gttaaatatg tcggtaataa ctattgaacg ctgtttccac cattgatttg
     1321 gagaaaacta aggcctattc catttgaaaa gcctatcgct tccaatcctg cctcctgccg
     1381 gtggaatcgg tttaaggcgt cctgccttaa actacggctt ttctgcattc cataagccaa
     1441 gttttcttag cccaatcaat gaagtcatcc gcttttcaat agtctattac ctggataaag
     1501 agttggcaca atgtgtaaat tttggttgat ccgcaaaaca tataagcgta agggaatgat
     1561 tgtaatgaaa tatatagtag ttttggggga tgggatggcc gataggccta taccggagct
     1621 gaataataag acccccctcc aatgtgccaa aaagccaaat atggatagcc tggccagccg
     1681 gggcgaaatg ggtcttgtaa ataccatccc cgaaggtatg cctcccggga gtgatgttgc
     1741 caacctgtcc accatggggt ttgacccccg cctctactac accggacgct ccccgttgga
     1801 agcggtcagc atgggcatag aacttgcaga taccgacgta accctgcgct gcaatctggt
     1861 aaccctatcc gaggataagg cctatgaaga taaaacgatg ctggattata gttccgatga
     1921 gatttcgtcg gaggaggcta agatacttat agacgatata ggggctcaac ttaatgggcc
     1981 tgacatttcc ttttattccg ggattagcta tcgtcattgc atggtatggt caggcggccc
     2041 tgtggatcta aatctgacac cgcctcacga tatattggaa aagcccatag gcgatttttt
     2101 acccaagggg aacacgaata aaattatttt agagatgatg gaaaaaagtc atgaaatttt
     2161 aaagaaccac cccgtcaatc tggccaggat ctccaagggt cttggcccag ccaattctat
     2221 atggctatgg ggagaaggta aaaagccctc ccttccaagc tttcaggaaa cctatgggaa
     2281 aaaaggttcc gtcatatcag ccgtggattt agttaagggc atcggcatat ccgcagggct
     2341 gagatctgtc catgtagagg gggctacggg aaatatcgat accaattttg taggcaaggc
     2401 agaagctgct cttgaggaat tgaagtccgg ccaggatttt gtatatatac acatagaggc
     2461 ccccgacgag tgcggccatc gccgggaaat cgaaaataag gttaaatcca tagagctgat
     2521 agataggcag gtcttaggaa cccttcttga agggctggaa caatacgaag attataggat
     2581 catgattctt ccggatcacc ccactccgct ggcccttaga acccatacct cagatcccgt
     2641 cccctatatc at
//
LOCUS       DASRNX010000215        12475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_200582,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000215 DASRNX010000000
VERSION     DASRNX010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12475
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200582"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VFD89_08420"
     CDS             complement(<1..1330)
                     /locus_tag="VFD89_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58238.1"
                     /translation="MKKSIIFLALIMALVFAFTACTGAKQPVKEPDKPADTPADDTPE
                     PADDEEPADEPDTADLEPYEIVYYLYSTKASDQDKVIEEAINEIIEPKFNATIDFVMV
                     AGGDWDEKAKVALNSGEKIDIFWTPEWMGYMDNISAGVLLPLDDLMAEYAPQTVVDLG
                     DFIPANIVDGALYGVSTNKELCVPGGLVWNEELTEKYDIDVTQVNTVEDLEPILYEFK
                     NSEEYAAGTYPLLSTGGWSAFDPFIQGFMSDMSSITMYIGEPGATDGEPVNQWTEDDP
                     MAGRNRREIMAKWNKDGLLHPDSHLTSFSNIDYLNNGAFLVSTDFVLKGGQVKAKELI
                     AQSGNPELKLVEHQTSASVNVTTHAGGSMLGIPITSEDPARAMMYINEMHQNEELLNI
                     MAWGIEGTHYDLNDDGYVIPKAANGWSDSHGGMWSLGNQFKQKLSASEDPL"
     gene            complement(1415..2377)
                     /locus_tag="VFD89_08425"
     CDS             complement(1415..2377)
                     /locus_tag="VFD89_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZJ58239.1"
                     /translation="MTSMTNITPALPKKKTSKYKDMNQLSAGANLVWNVLLIIGCLFC
                     IVPLWIVIVASFTDNATLVMKGYKFWPAVFSGSAYKFLLSSGGGSMLVAYKNTVAATL
                     LGTVLCVISVGLYAYPLSRTDFRYRRFFTFYSFFSMVFNVGTVAFYMLCTSILQINNT
                     LYALFLPQSFSAYWVIIMRTFYRSNIPDSVIESARIDGAGEWRTLLQIVAPLAIPGFA
                     TVALFAAVGIWNNYFNALLLINKRELYNLQYTLAEILKSIQFLKEMAASSGGAVDTIK
                     NLGDLPSESFRMAMAVVTMGPIILAYPFFQKYFVKGLTIGAVKG"
     gene            complement(2426..3430)
                     /locus_tag="VFD89_08430"
     CDS             complement(2426..3430)
                     /locus_tag="VFD89_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZJ58240.1"
                     /translation="MKATALSKETRKVVKKRSWGTGEVKRHLAYLIMLLPGIIFLFVF
                     SYLPLPTIIMAFKKYKLAIPPAGHWLQNKFLYSLFFKSPWVGLENFKFILNAPLFPVF
                     MRNTIGYNLVFMAIGLVAAVALAVGINELRQRKAAKVYHTILFLPYFLSWIIVTYILF
                     AFISPAGVIPQAFTALGMTPPQLYNNAKAWPIIYVIFNLWKYAGNGSIIYLSTITGFS
                     QELYEAASIDGAGKWKQFRYVTFPQLVPTIILLQILAVGRILNADFDMFYNLRNGASA
                     AYQNSLTIDVYVYEMIKSNPSKLYYPAAAAFFQSLIGFVLVLTTNSIVRKVEPEMAMF
                     "
     gene            complement(4237..5679)
                     /gene="larC"
                     /locus_tag="VFD89_08435"
     CDS             complement(4237..5679)
                     /gene="larC"
                     /locus_tag="VFD89_08435"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004819410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HZJ58241.1"
                     /translation="MKTMYIECNMGAAGDMLMAALLELHPEPDSFIKRMNGLSIPGVT
                     VIRETSVKCGITGTHIRVQVGGLEEESHDVPVGHTHHHHHGDEHHHGNGHEHGGVHHH
                     SHDHHNEDDHDAHEHDHTHGHGYDHEHHHSHEHHHAHEHHHAHEHGHAHEHHHAHEDG
                     HTHEHHHSQGHGHAGMTEIEDTVNSLPLSDKVKADVMAVYGLIAEAESRAHGQPVEAV
                     HFHEVGAMDAVADIVGVCLLIEELAPERVIVSPVHVGSGQVKCAHGILPVPAPATAYI
                     LKDVPSYGGRIEGELCTPTGAALLKHFAHEFGDRPLMTTVSVGYGMGKKDFPAANCVR
                     IFLGKSREQAPDIVKVECNLDDMTGEELGFALEILMENGALDAYIIPIQMKKSRPGHV
                     FVCLCRPEDEDRIAGLMLKHTTTFGVRVSRCRRYTLERTVETIETKYGGVSVKTGIGY
                     GTIKSKAEYEDLARLARENDMSILEIKKHL"
     gene            complement(5676..6434)
                     /gene="larB"
                     /locus_tag="VFD89_08440"
     CDS             complement(5676..6434)
                     /gene="larB"
                     /locus_tag="VFD89_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HZJ58242.1"
                     /translation="MDQRETLKMLGDVRKGIITPEEALKRLKTAPFDDLGYAKIDNHR
                     GIRQGVPEVIYGGGKTASQICGIVSKMLENGANDIIISRLAPESAKILEKQCDLEYHP
                     IPRFGIVNRDMGRERIGSIVVASGGTSDMVVCEEAALTAEVLGNEVIRLYDIGVAGLH
                     RLLSRLDVLTNARVIIAVAGMEGALPSVIGGLVSCPVIAVPTSVGYGANLGGIAALLS
                     MLNSCASGVSVVNIDNGFGAGFLASRINQMEGIK"
     gene            complement(6456..7232)
                     /gene="larE"
                     /locus_tag="VFD89_08445"
     CDS             complement(6456..7232)
                     /gene="larE"
                     /locus_tag="VFD89_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HZJ58243.1"
                     /translation="MDLKTKFSAVPRALREILEGIPKAALGFSGGVDSSYLLYAAKVC
                     SVDIRGYYASSQFQPQFELDDARKVAQQVGAEMEVISLDVLSNNIVRENPADRCYHCK
                     GVIFSAILDRAAADGYSVLMDGSNASDDADDRPGMRALSELKVLSPLRESGLTKDQIR
                     EYSKQAGLFTWDKPAYACLATRIPTDTPLDAETLCKVEQAENKLFEMGFTDFRARVMG
                     NAAKLQMPQGQILRAARRHSELVEALSPWFEDVLLDLKPR"
     gene            complement(7374..8000)
                     /locus_tag="VFD89_08450"
     CDS             complement(7374..8000)
                     /locus_tag="VFD89_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004223279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZJ58244.1"
                     /translation="MRLEANDISFRYTIKTPFILKDVSFSVEEGERVGVVSPSGYGKT
                     TFVKVLAGHEKPTKGQVLLGGEPLAPNGPSPIQLICQHPENAINPRWKMNKVLEEAGQ
                     LRTGVLEAMGIEPEWLNRYPRELSGGELQRFCVARGLCTDTRFLIADEISTMLDVITQ
                     AHIWRYLLSEAEERGIGLVVVTHNPALAKRVCTRIVDLRDINHIESVY"
     gene            complement(7990..8928)
                     /locus_tag="VFD89_08455"
     CDS             complement(7990..8928)
                     /locus_tag="VFD89_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016279388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58245.1"
                     /translation="MCENNLPLLEVNNLSVSFRMYDRVLGQRDLKVIQDLAITVNRGE
                     VLAVVGSSGSGKSLLAHAVLGLLPKNAAVSGTMFYNGTPLCERVQKRLRGNEIALIPQ
                     SVTYLDPLMQIGKQARGTYKGPKGVGLQRKVFQRLNLATETERLYPFQLSGGMARRLL
                     FSTAVISDARLIIADEPTPGMHLDQALEALSILRELADAGKGIVLITHDIDLALRFAD
                     KIAVFYAGTTLEETPVADFIKGPHGLRHPYSKALWHALPQNDFTPIEGFQPYAGDLPF
                     GCLFAPRCPLKTPECEAENPPMKKLRDGKVRCVHAT"
     gene            complement(8940..9800)
                     /locus_tag="VFD89_08460"
     CDS             complement(8940..9800)
                     /locus_tag="VFD89_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZJ58246.1"
                     /translation="MNNQLAVKDPGQIIGLNRRVKTVIISGILLGIIVGVFLAGVLIG
                     DAAMTVDFSRKGLGPSWRYPFGTDWLGRDMLLRTVKGLSISIMVGSIASAVSAVLATI
                     IGIAAATGKRWVDSSIGWLIDLVMGVPHLVLLILVSFACGRGLTGLLVGIAVTHWTGL
                     ARLVRAEVLQIRGQQYITISRHLGQSKGWILRHHILPHVLPQFVIGLILLFPHAILHE
                     SSLSFLGYGLPPEQPAIGIILAESMKYLTGGMWWLAFFPGITLVLVVILFDKLGENLK
                     MLLDPYSAQE"
     gene            complement(9778..10800)
                     /locus_tag="VFD89_08465"
     CDS             complement(9778..10800)
                     /locus_tag="VFD89_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZJ58247.1"
                     /translation="MEMKGTKAIKVGKIAGKYLFRILTLLLATSLVAFILVSLSPIDP
                     VQQYVMGAGNVSMEQREAISEYWGLDAPPTERYFNWLSALLHGDMGTSLLYRQPVARI
                     ISERFINTFALMMTSWIISGIMGFALGCIMGIWRGKWVDSILKRICLVLCSVPTFWLG
                     LVFLLIFSVWLGWFPIGFSSPVGMVDGDVSVWQRLYHLALPALTLSLISFANVALHTR
                     QKLVDVLESDYVLFARARGGDTWTIMKRHGFRNILLPAVTLQFASFAELFGGSVLAEN
                     VFSYPGLGSAVSAAGLNSDVPLLLGITLFSAFFVFLGNFIANIIYGVVDPRIGEERPY
                     EQSTGG"
     gene            complement(10791..12407)
                     /locus_tag="VFD89_08470"
     CDS             complement(10791..12407)
                     /locus_tag="VFD89_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZJ58248.1"
                     /translation="MKKLSAIILSVVLCVAALAGCGGNAGEIQSDPSKGSANNDIKDE
                     VIVAIESEPENGFDPTTGWGHGTTPLVQSTLVEYTQDMRIENDLATDYTISEDGTTWI
                     FAIRDDAYFTDGNPVKASDIAFTFNTAKNSQSSLDFTYLVDCEATGDYEVTFTLNAPN
                     STFINTIATTGIVPEHAYSDNYANEPMGSGPWKLVQWNKGEQVILEANEDYYGTIPAI
                     KKVVLVFMDEDAAFAAAKAGEVDVALTSATHATRDIDGMRIEAVTTLDNRGFTLPTIP
                     AGATSESGHPAGNDVTSNLAIRKAIAYSIDRERIARDAVNGFATPAYSENDGMPWNNP
                     DVRIETDIEKAKKLLADDGWEDSDGDGILEKDGLRAEFSCIYPSGDSVRQAVAMAAAA
                     QAEEIGINIVVEGVSWDEISRRMFSDAVLMGWGSSNPYTSYLLYHSQNKLRDDYYNPG
                     GFDNAVVDGYLDAALHAATTEEAYEKWKLAQWDGTTGTSMQGECPWVWLVNIKHIYYV
                     AKGLDIGDQQLHAHGASWPLVQNLRDWRWK"
BASE COUNT         3196 a         3228 c         2821 g         3230 t
ORIGIN      
        1 tcaacggatc ctcggaagca gatagctttt gcttgaattg gttacccaat gaccacatac
       61 cgccatggga gtcggaccag ccgttggcag cttttggaat tacatagcca tcatcgttca
      121 ggtcataatg tgtgccttca ataccccaag ccatgatatt gaggagctct tcattctgat
      181 gcatctcatt gatatacatc atagcacgtg caggatcctc ggaggtgatt ggaataccca
      241 gcatggatcc gccggcatgg gtggttacgt taacagatgc ggaggtctga tgctcaacca
      301 acttaagttc ggggttaccg gattgggcta taagttcctt ggccttaacc tgtccgccct
      361 tcaatacgaa gtcggtggat accaggaatg caccgttatt cagatagtca atgttgctga
      421 aggaggttaa gtgggagtca ggatgtagta agccgtcctt gttccacttg gccatgattt
      481 cacggcggtt tcttcctgcc atgggatcgt cttcggtcca ttgatttaca ggttcgccat
      541 cggtagcacc aggttcaccg atatacatgg tgatggagct catgtcggac ataaagccct
      601 ggatgaatgg gtcaaatgca gaccagccgc cggtagataa cagcggataa gttccggctg
      661 catattcttc actgttctta aactcgtaaa gaatcggctc taggtcctca acggtattaa
      721 cttgagttac atcgatatca tacttttcgg tcaattcttc attccatact aatccgccgg
      781 gaacgcatag ttccttgttg gtggatacac catagagggc tccgtcaacg atgttggcgg
      841 gaatgaagtc acccaggtct acaacggtct ggggtgcata ctcagccatc agatcatcta
      901 agggcagcag aacgcctgca cttatgttat ccatatagcc catccactca ggggtccaga
      961 agatatctat cttctcaccg gagttaaggg caactttggc cttttcgtcc cagtcaccgc
     1021 cggctaccat aacaaaatcg atggtggcgt tgaacttggg ctcgataatt tcattgattg
     1081 cttcttcaat gaccttatcc tggtcggatg cctttgtaga gtatagatag tacacgatct
     1141 catagggttc aagatcggcg gtatcgggct cgtccgcagg ctcttcgtcg tctgccggtt
     1201 ctggggtgtc atcagctggt gtatcagctg gcttatccgg ctcttttact ggctgctttg
     1261 cgccggtgca ggcagtaaat gcgaacacta acgccataat aagtgccaga aaaataatgc
     1321 tttttttcat tcttgtttct cctcctcgtg attatatgtt aaagggaaaa tccctttaat
     1381 cgctctagtt aaatttgtac tgggttaatt cccatcatcc ctttacagca ccaatagtta
     1441 ggcctttgac aaagtatttt tggaagaagg gatatgccag aattatagga cccatggtaa
     1501 caactgccat agccatcctg aaggattctg aaggaaggtc ccccagattc ttgatggtat
     1561 cgacggcgcc accgctggat gctgccatct cctttaggaa ttggatggac tttagaatct
     1621 ctgccagggt atattggagg ttatataatt ccctcttgtt tataaggagc agagcgttaa
     1681 aatagttatt ccaaatgccg acggcagcga ataaagctac ggtagcaaag cccgggatag
     1741 ccaggggtgc tacgatttgc aataatgttc tccactctcc cgcaccatct atacgagcag
     1801 actcaataac ggagtcggga atattggatc tatagaaggt tcgcattatg attacccagt
     1861 atgcgctgaa gctttggggt aaaaataagg cataaagcgt gttgttgatc tgcaggatcg
     1921 aggtacacag catataaaat gcgaccgtac cgacgttaaa taccatggag aaaaaggaat
     1981 agaaggtaaa gaagcgccga tatcgaaagt ccgtcctgga gagtgggtag gcatataatc
     2041 ctacactgat tacacataaa actgtaccta gtaaggtggc tgccacggtg tttttataag
     2101 ctaccaacat agatccgcca ccggaggaca gtaggaattt gtaagctgag cccgaaaata
     2161 cggcaggcca gaatttatag cctttcataa ctaaggtcgc gttatctgta aaggatgcaa
     2221 ctataacaat ccaaagcggg actatacaaa acaggcaacc tatgatgaga agcacattcc
     2281 aaaccagatt ggcccctgct gaaagctggt tcatatcttt atatttggat gtctttttct
     2341 tgggcaaagc aggtgttata ttcgtcatgc tcgtcatata agggttcctt ctttctatat
     2401 atatttctta acaaagcagt tccgattaaa acatcgccat ctctggttct accttgcgca
     2461 cgatactatt ggtcgtaaga acaagaacaa atccgatgag agattggaaa aaagcagccg
     2521 ctgcagggta ataaagtttg gacggattgc tctttatcat ctcatataca tatacatcaa
     2581 tggtcaggga gttctgataa gcggcgctgg caccgttacg cagattgtag aacatatcaa
     2641 aatcggcgtt tagaatacgg ccaacggcca gaatctgtaa aagaatgatg gtgggaacca
     2701 attggggaaa ggtaacatac ctaaattgtt tccatttgcc cgcgccatct attgaagccg
     2761 cctcatatag ctcctgagaa aatcctgtaa tggtggaaag gtaaatgatc gaaccgtttc
     2821 cggcatattt ccataagttg aaaatcacat agataatagg ccatgctttc gcattattgt
     2881 acaattgcgg aggggtcata ccaagagctg taaacgcttg gggaataaca cctgcaggac
     2941 ttatgaatgc gaatagaatg taagttacaa taatccaaga aagaaaataa ggtaaaaata
     3001 gtatagtatg atacactttt gcggcctttc tttggcgaag ctcgttgatc ccaacagcca
     3061 gggcaacggc ggccaccagg cctattgcca taaatacaag gttgtagccg atggtatttc
     3121 tcataaatac gggaaacaag ggggcattca atatgaactt aaagttctca agtcccaccc
     3181 atggactttt gaaaaacaag gagtacaaaa acttattttg cagccagtgc ccggccgggg
     3241 gtatggccag tttatatttt ttaaaggcca taataatagt gggcaggggc aagtagctaa
     3301 aaacaaataa aaatataatt cccggtaata acataatcag ataggccaaa tggcgcttaa
     3361 cttcaccggt cccccaacta cgttttttaa caacttttct cgtctcttta gacaatgcag
     3421 tcgctttcat cacatctcct cctgggagag ctatcattcc aaatagccca taataaataa
     3481 caatttatcc ttaagcaagt actatatcca tattttgcca agctttatat cctatataac
     3541 caatattgat gcttttattg caacatgacc cagatgtaaa aactatattg agatatccca
     3601 caaagcatga tgcattacat agagtctgta tcggaatgct gttcgtttca ccgcttctcg
     3661 gtgatatagg acagtaaatg atggcatcat aggtgcccct tatcaaatcg ggcaagaaag
     3721 aatcatatat ccgacaaaag tatatatatc aaatagtcaa ggtattgata aatatacaaa
     3781 acctctgaat tttcgccaat gttttcgatc cctccagctt aaaattcaaa gatagagaaa
     3841 tttaaaaccc tataaactat ttgtacatct tatatcatta acatctgatc ttcctgtgca
     3901 tcggtggcgc ttcatacgcc attttgcatg gccgatatgc aatatacaga ctatgcacat
     3961 ataataacgt ataacaaagt gtgtgtcaat aggaaaaatg aaatttttct accaaaaggt
     4021 gacatatggg gaaatatact gtttgatacc tgatctctct tatgggtatc cctttgtttt
     4081 acaactaatc cctctcccat ggatatcccc acccatggtt gtacaagggc ctttaagtgc
     4141 atgacccagg acttttaacc cttttatcct ctaggtcccg gcgtttaaac tactgtgttt
     4201 aaacgccggt taaattacga ccccacggtc accctactag agatgctttt taatttccaa
     4261 gatactcata tcattttccc ttgccagcct tgccaaatcc tcgtactcag ccttactctt
     4321 tattgttcca tagccgatac cggttttaac ggagaccccg ccatatttcg tctctattgt
     4381 ctctacagta cgctccaatg tataccgccg gcaacggcta acccgtacac caaaggtagt
     4441 agtatgcttt aacatgagcc cagcgatcct atcctcatct tccggcctac acaagcatac
     4501 aaatacatgg cccggacgac tcttcttcat ctgtatgggg attatgtagg catccagcgc
     4561 tccgttttcc atcaaaatct ccaacgcaaa gcccagctct tcgccggtca tgtcgtctag
     4621 gttacactcg acctttacaa tatcaggggc ttgctcccga ctcttcccaa ggaaaatacg
     4681 gacacaatta gccgcgggaa aatccttttt gcccattccg tatccgacgg aaaccgtggt
     4741 cattaaaggt ctgtccccaa attcatgggc gaagtgtttt aaaagggctg cgccggtagg
     4801 ggtgcagagt tcgccctcaa tccgcccgcc atagcttggc acgtccttta atatgtaagc
     4861 tgtcgcaggt gcgggtaccg gcaatatccc gtgggcacat tttacctggc cgctgccaac
     4921 atgtacgggc gatactatta cccgctccgg agccagctct tcaattaaga gacatacacc
     4981 cactatatca gccactgcgt ccatggcacc tacctcatga aaatgtacgg cttctacggg
     5041 ttggccgtga gccctgcttt cagcctctgc gattagtcca tataccgcca ttacatccgc
     5101 ctttaccttg tccgacaaag gaagagaatt tacggtatcc tcgatttctg tcattccggc
     5161 atgaccgtgc ccctgggaat gatggtgttc gtgcgtatga ccatcttcat gagcatgatg
     5221 gtgttcatgg gcatgtccat gttcatgggc atgatggtgt tcatgggcat gatggtgttc
     5281 atgggaatga tggtgttcgt ggtcataacc gtgcccatga gtatgatcgt gttcgtgagc
     5341 atcatggtcg tcttcgttat ggtgatcgtg actgtggtgg tgaacgccac catgttcatg
     5401 cccgttccca tgatgatgtt catccccatg atggtggtga tgtgtatgtc ccaccgggac
     5461 atcatgactt tcctcctcca gtccgccaac ctgtactctt atatgggtgc ccgttattcc
     5521 gcatttgacg gatgtctccc ttatgactgt aacgccgggg atactcaatc cgttcatcct
     5581 ttttataaaa ctgtcaggtt ccggatgcaa ctctaaaagt gcggccatca acatatcacc
     5641 ggccgccccc atattgcatt ctatatacat ggttttcatt ttataccctc catctgattg
     5701 attcggctgg cgagaaaccc cgccccaaag ccattatcga tattgactac tgaaacaccg
     5761 gatgcgcatg aattaagcat ggaaagcaac gcggctatgc ctccaagatt ggccccatag
     5821 cctacactgg taggcacggc aattacagga cagctgacaa ggcccccgat tacgctggga
     5881 agggccccct ccatgccggc aacggcaata atcacccttg catttgtcag cacatccaaa
     5941 cgggaaagca atctatggag cccggctaca cctatgtcgt acaggcggat aacttcgttc
     6001 cccaatacct ctgccgtgag ggcagcctct tcgcaaacga ccatatcgct tgtacccccg
     6061 ctggccacca caatggagcc tatcctctcc ctccccatat cgcggttcac tatgccaaac
     6121 ctgggaatag gatgatattc aaggtcacat tgcttttcca aaattttagc ggattcaggt
     6181 gcaaggcggc ttatgatgat gtcgtttgca ccattttcca gcatctttga tacaatgccg
     6241 caaatctggc tggcagtttt cccgccacca tagatcacct ccggtacgcc ctggcgtatg
     6301 ccccgatggt tgtcgatctt cgcatagccc aagtcatcga agggggctgt tttcagccgt
     6361 ttcaaggcct cctcaggtgt gatgatgccc tttctgacat ctcccagcat tttaagcgtt
     6421 tctctctggt ccattaaagc atttctcctt ttcccttatc ttggttttaa gtccagcaac
     6481 acatcttcga accaaggtga cagtgcctcc accagctcac tgtgccgccg ggccgccctt
     6541 aggatttgcc cttggggcat ttgaagtttt gcggcgttgc ccattacccg tgcacgaaaa
     6601 tcagtaaatc ccatctcaaa caacttattt tccgcctgct cgaccttgca gagggtttcg
     6661 gcatctaagg gtgtatcggt gggtattcgt gtggccaggc aagcataggc aggcttatcc
     6721 catgtaaaca atcccgcttg tttggaatat tcccgtatct gatctttggt aagtcccgat
     6781 tctctcaagg gggaaagcac cttgagttcg gaaagagccc gcatccccgg cctgtcatcg
     6841 gcatcatcgc tggcattgct gccatccatc agcaccgaat aaccgtctgc cgcagcccta
     6901 tccagtatcg cagaaaaaat cacccctttg caatggtagc aacgatcggc ggggttttcc
     6961 cttacaatgt tgttggagag cacatcaagg gaaatcactt ccatttcggc tcctacctgc
     7021 tgggcaacct tccttgcatc atccagttca aactggggtt gaaactggga acttgcgtag
     7081 tagccgcgta tatctacact gcagacttta gcggcataca ataggtaact ggaatccacg
     7141 ccgccggaaa atcccagggc agcctttggg atcccttcta gtatttccct aagagccctc
     7201 ggaacggcgg aaaattttgt ttttaagtcc aaattaagtc cctccctatt atttcaaaat
     7261 tataagcccc tttaaaatac gatccttgag gtaagctcat attcggtttc ggtttaagta
     7321 tatgatcctt aaagtaagag catatccgat gcaaagattg gatttgctca tatttaatat
     7381 acggattcta tatggtttat gtcccgcaga tccacaatac gggtacacac ccgctttgcg
     7441 agggccgggt tatgggtaac caccaccagc ccgatacctc gctcctcagc ttctgaaagc
     7501 aaatatcgcc atatatgtgc ttgggtaata acatccaaca tggtgcttat ctcatcggcg
     7561 atcaaaaatc tggtatcggt gcagagccca cgggctacac agaatcgctg caattccccg
     7621 ccggaaagtt cccgtggata acgattcagc cactccggtt cgattcccat agcctctaaa
     7681 accccggtcc gaagctgccc cgcctcctcc aataccttat tcatcttcca gcggggattg
     7741 attgcatttt ccggatgctg gcagatcagc tgtatcggcg aaggaccatt tggcgcaagg
     7801 ggctccccac ccaaaagcac ctgtcctttt gtgggtttct catgtccggc caggaccttt
     7861 acaaaggtgg ttttgccgta gccgctgggc gataccacgc ctacccgttc cccctcctcc
     7921 actgagaagg acacgtcctt tagaataaat ggggtcttga tcgtgtatct gaaagagata
     7981 tcattcgctt caagtcgcat gcacgcacct caccttcccg tcccgcaatt ttttcattgg
     8041 cgggttttcc gcttcgcact ccggggtttt cagcgggcac ctgggcgcaa aaaggcaacc
     8101 gaagggcaga tcccccgcat atggctggaa accctctatg ggcgtgaaat cattctgcgg
     8161 aagcgcatgc cacagcgcct tgctataggg gtggcggagc ccatggggcc ccttgataaa
     8221 atcggctaca ggtgtttcct ccagcgttgt gcctgcataa aacacggcga ttttgtcggc
     8281 aaaccggaga gcaaggtcta tgtcatgggt gatcagtact attcccttgc ccgcatcggc
     8341 aagctcccta aggattgaca atgcctcgag ggcctgatcc agatgcatac cgggagtggg
     8401 ttcgtctgcg attataagcc gggcatccga gataacggcc gttgaaaaga gcaggcgccg
     8461 ggccataccc cccgagagct gaaaggggta gagcctttcg gtctccgttg ccagattgag
     8521 cctctgaaag accttcctct gcaaaccgac acctttggga cccttataag tacctctggc
     8581 ctgtttgcct atctgcatca gcggatctaa ataggtgacg gactggggta tcaaggcaat
     8641 ctcatttccc ctcagccttt tctggacccg ttcgcagagt ggcgtcccat tatagaacat
     8701 cgttccgctg actgcagcgt tttttggcaa tagccccagc acggcatgag ccaaaaggct
     8761 cttcccgctg ccggatgagc ctacgacagc caatacctct cccctattta ccgttatagc
     8821 caggtcctgt ataaccttta aatccctctg ccccaatacc ctgtcataca ttcgaaaaga
     8881 tacggaaagg ttgttcacct caaggagcgg cagattattt tcgcacataa tatttccccc
     8941 tattcctgag cactgtacgg atcaaggagc attttcagat tctcccccag cttatcaaat
     9001 aaaattacga ccaagaccaa ggtgatgcca gggaagaatg ccaaccacca catacccccg
     9061 gtgagatact tcatgctttc ggcaagtatt atcccaatgg ccggctgttc cggcggcaag
     9121 ccatagccta aaaatgacag agaggattcg tgcaatatgg catgggggaa cagcaatata
     9181 agccctataa cgaattgcgg caacacgtgg ggtaggatat ggtgacgcaa aatccagccc
     9241 ttggattggc ccagatgacg ggagatggta atgtattgtt ggcctcgaat ttggagcacc
     9301 tcggcccgca ccagcctggc aagaccggtc caatgggtga ccgcaatccc tactaacagg
     9361 cccgtaaggc ctcgtccgca tgcaaaggag acgagaatta gcagcacaag gtgaggcacc
     9421 cccattacaa ggtcgataag ccagcctata gagctgtcca cccacctttt tcccgtagcg
     9481 gcggcaatgc cgatgattgt ggccaggaca gcactgacgg cagatgcgat gctccctacc
     9541 atgattgaga tggatagacc tttgacagtg cgaagcaaca tatccctgcc cagccaatcg
     9601 gtaccgaagg gatatctcca ggagggtcca agccctttac gggaaaaatc cacggtcatt
     9661 gctgcatccc ctattaagac acccgccaaa aaaacgccta cgattatgcc aaggaggata
     9721 cctgagataa tcaccgtttt aacccggcgg tttaaaccaa ttatctgtcc cgggtcttta
     9781 accgccagtt gattgttcat agggtctctc ctctcctatc cgcgggtcca ccacgccata
     9841 gatgatattg gcaatgaaat tgcctaaaaa gacaaagaag gcgctaaata gggttatccc
     9901 cagcaagagg ggaacatccg aattcaatcc cgcggcggag accgcagagc caagccccgg
     9961 atatgaaaaa acattttccg ctagtacgct gcccccaaac aactccgcaa aggaagcgaa
    10021 ttgtaaggta acggcaggca acagtatatt tctgaagccg tgacgcttca tgattgtcca
    10081 agtatccccg cccctggccc gcgcgaataa cacatagtcg ctctccaata catccaccag
    10141 cttttggcga gtatggagtg ccacgttggc aaaggagata agactcaggg taagcgcagg
    10201 aagggcaagg tgatacagcc gctgccagac ggacacatcg ccgtccacca tgcctacggg
    10261 gcttgaaaac ccaataggaa accaaccgag ccataccgaa aatatcaaca ggaataccag
    10321 ccccaaccag aaggtgggca cagaacagag taccagacat atgcgcttta ggatgctgtc
    10381 aacccatttt ccccgccata ttcccatgat gcagcccagg gcaaagccca tgattccgga
    10441 aataatccag gaggtcatca tcagggcaaa cgtatttata aatctctcgg atataattcg
    10501 ggccaccggt tggcggtaca gtagggatgt tcccatatca ccgtgtaaga gcgccgataa
    10561 ccagttaaaa tacctctccg taggcggagc atcaagcccc caatattccg aaattgcctc
    10621 cctttgctcc atgctcacat ttcccgcacc cattacatat tgctgtaccg ggtctatggg
    10681 tgaaaggctg acaaggataa acgctacaag ggatgtggca aggagcaatg tcagaatccg
    10741 aaagaggtac ttgccggcga ttttgcctac cttaattgcc tttgttccct tcatttccat
    10801 ctccagtcgc gaagattttg taccaggggc cagctggcgc cgtgggcatg gagctgttga
    10861 tcgccgatat ccaagccctt tgcgacataa tagatatgct ttatattcac aagccacacc
    10921 caagggcatt ccccctgcat ggaggtgccc gtggtaccgt cccactgggc aagtttccat
    10981 ttttcatagg cctcttctgt tgtggcggca tgcagcgccg catctaagta tccgtcaaca
    11041 acagcattgt caaagcctcc gggattgtag taatcatccc gaagcttgtt ttgggaatga
    11101 tataataggt aactggtgta ggggttggaa gaaccccagc ccatgagtac agcgtcgctg
    11161 aacattcgcc ttgatatttc gtcccagctt acgccttcca caactatatt tattccgatt
    11221 tcctctgcct gcgctgcagc cgccattgct acggcctggc gcacagaatc gccgcttggg
    11281 tagatacagc taaattctgc cctaagaccg tccttttcca aaataccgtc accgtctgaa
    11341 tcctcccagc cgtcatccgc caggagcttt tttgcctttt ctatatcggt ttcaatcctt
    11401 acatccggat tattccatgg catgccgtca ttttcgctgt aggcaggagt ggcaaagcca
    11461 tttacggcat cccttgcaat ccgttcccta tctatactat aggcgatagc cttgcggata
    11521 gccaggttgc ttgtcacatc gttgcccgcc gggtgcccgc tttcgctggt tgcgcctgcc
    11581 ggaatagtcg gaagggtaaa tccgcggttg tcaagggtcg tcaccgcctc gatacgcata
    11641 ccgtcgatat cccgggtggc gtgggtagcg ctggtaaggg caacatctac ctctcccgcc
    11701 ttggcagcgg caaatgcggc atcttcgtcc ataaatacca gaacaacctt tttaatagcc
    11761 ggtatggtcc cataatagtc ctcattggct tccagaatta cctgctcgcc cttattccac
    11821 tgtaccagct tccaggggcc gctgcccata ggttcattgg cgtaattatc cgagtaggca
    11881 tgctccggta ctatacccgt agtcgcaatg gtattgataa aggtagaatt aggcgcgttg
    11941 agggtaaagg taacctcata atcacccgtg gcttcgcagt ccaccaggta tgtaaaatca
    12001 agggaagatt ggctattctt tgccgtattg aaggtaaagg ctatatcgga agcctttaca
    12061 gggttaccgt cggtaaaata ggcatcgtcg cgaatagcaa aaatccatgt agttccgtct
    12121 tcgctgatgg tataatccgt agccagatca ttttctatgc gcatatcctg ggtatactcc
    12181 accaatgtgc tttgaaccag cggcgtagtg ccgtgtcccc aacccgtagt cggatcaaat
    12241 ccgttctccg gttcactttc aatggccacg attacctcat ccttaatgtc attgttagcg
    12301 ctgccctttg acggatcgga ctgaatctct cctgcattgc cgccgcagcc ggctaaagcc
    12361 gctacacata gcactaccga taggataatg gcacttagct ttttcattat acatcttctc
    12421 ccgttttaaa aattacacaa ccttattatt cagtggaggc agctaaatcc attaa
//
LOCUS       DASRNX010000216        11898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_257995,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000216 DASRNX010000000
VERSION     DASRNX010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11898
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257995"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFD89_08475"
     CDS             <1..328
                     /locus_tag="VFD89_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58249.1"
                     /translation="EQAMSRLKNNLKNKTRRRMIFFSLFSTAVLFISVLVVTNNTMVR
                     MTDLTREKSPVLAQDMGTYITKKVNSLANNLREISVHEFGQNIGDMVEKVIDIMKSLK
                     FRWQGL"
     gene            545..2350
                     /gene="lepA"
                     /locus_tag="VFD89_08480"
     CDS             545..2350
                     /gene="lepA"
                     /locus_tag="VFD89_08480"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZJ58250.1"
                     /translation="MSNARQEKIRNFCIIAHIDHGKSTLADRILEITGMLTEREMEKQ
                     VLDDMDLERERGITIKSKAVRLSYRDEEGEEYILNLIDTPGHVDFAYEVSRSLAACEG
                     AILVVDASQGIEAQTLANVYLALEHDLEIVPVINKIDLPSARPDWVKKEIEDVIGLDA
                     GDAPMISAKNGIGIEGVVEKIVSSIPAPIGEDDAPLKALIFDSFYDNYRGVIAYIRIK
                     EGKLKVGDKIRMMATGKEFEVTEVGIFRPSPTSVPELMAGEVGYVAASIKNVRDTRVG
                     DTITHSDNPAAEPLPGYRRVNPMVYSGVYPADGARYEELREALHKLQINDASLSFEPE
                     TSMALGFGFRCGYLGLLHMDIIQERLEREYDLDLVTTAPSVIYKVIMNDGTKQDISNP
                     TSLPDPADIEHMEEPVVNAQIMAPTEYVGAIMELCQERRGIYKDMEYMEETRVLITYE
                     LPLNEIIYDFFDALKSRTRGYASFDYELIGYQQAELVKLDILLNGEPVDALSLIVHNE
                     KAYTRGRGIAEKLKELIPRQMFEIPIQAAIGNKIIARETIKALRKDVLAKCYGGDISR
                     KRKLLEKQKEGKKRMRQVGSVEVPQEAFMSVLKFD"
     gene            2368..3510
                     /gene="hemW"
                     /locus_tag="VFD89_08485"
     CDS             2368..3510
                     /gene="hemW"
                     /locus_tag="VFD89_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZJ58251.1"
                     /translation="MQADTIGLYVHFPFCLRKCLYCDFPSYAHKDYLMGEYLTALKVE
                     IDNIKDKIGHRQINTVFLGGGTPTLFHEKELVWLLDYIKQGSTIRHGAEITIEANPET
                     LSPDMLEALKQGGFNRLSIGMQACQDEQLKALGRTHSIGDVERGVNWAKKAGFHNINL
                     DLMFGLPNQTTDHWMESLVRAVGMGVEHISTYALIVEEGTPFFELASRGGLNTPSEDV
                     EREMYHRGAKYLTSLGYNHYEISNFAKSGKECKHNLIYWHNNEYIGVGSGAHSFLHGR
                     RRWNYADIHQYIDEVRKNGDGTEQWEVISPSDQRFETIMMGLRLNSGVSKKVFKKRFG
                     HDLGYYYGSIIETLKIEGLLIETDSNIHLTARGMDLQNSVLLHFME"
     gene            3610..4647
                     /gene="hrcA"
                     /locus_tag="VFD89_08490"
     CDS             3610..4647
                     /gene="hrcA"
                     /locus_tag="VFD89_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZJ58252.1"
                     /translation="MELGERKLHILQAIIDDYIISGEPVGSRTIAKKSGIAISSATIR
                     NEMADLEEMGYLEQPHTSAGRIPSDKGYRLYVDRLIKAEGLSINEAKYIKDIYDRKTR
                     HIEQVIFQTSKILSDITNYTAVVLGPKLSRVLIKHIQLVPIDAYYALLMVVTNTGIVK
                     DAIISIPQGVGMDYLARISNILNDLFKDQSLGEISLEPLIEMREGMIQNRDFFNSLVD
                     ALTESIDIGDISEVYLGGMTNIFNYPEYHDIFKAKEFLNLMEQKELLYRLLARSQDNG
                     VSVTIGEENQCEELREYSIITATYNINGKTLGTMGIIGPTRMEYSKTISVMDFMGKTL
                     SNYLTRLFSKE"
     gene            4782..5375
                     /gene="grpE"
                     /locus_tag="VFD89_08495"
     CDS             4782..5375
                     /gene="grpE"
                     /locus_tag="VFD89_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZJ58253.1"
                     /translation="MGKDKREKNTEDILGMDYGQEDRAEEITPDGGSECTGEAKELWL
                     LEEQLQKAGEERDMYLSMAQRLQAEFDNFRKRNRAAVSAAHQDSVYETVELFLPILDN
                     LQRGLESPGNNESTQVHAIYKGVEMVTKQFTDILEKLGIEEIEALDQPFDPTYHNAVM
                     QVEAEYEEQKNKVVEVLLKGYKGKDRVIRYSMVKVAI"
     gene            5581..7407
                     /gene="dnaK"
                     /locus_tag="VFD89_08500"
     CDS             5581..7407
                     /gene="dnaK"
                     /locus_tag="VFD89_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZJ58254.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGEAVVIPSAEGGRTMPSVVAFSK
                     TGERLIGQVAKRQAITNPEHTIMSIKRDMGSDRKVRIEDKTYSPQEISAMVLTKMKQD
                     AESYLGEKVTQAVITVPAYFSDSQRQATKDAGVIAGLDVLRIINEPTAAALAYGLDKE
                     DNQKILVYDLGGGTFDVSIMEIGDGVFEVLATRGNNRLGGDDFDQRIIDYLADEFKKS
                     DGIDLRMDKMALQRLKEAAEKAKIELSSVLTTNVNLPFITADASGPKHLDVTLTRAKF
                     EEMTADLVEKSLSPLRAAIKDAGLSANEIDRVILVGGSIRIPAVQEAVKKVTGKDPHK
                     GINPDECVAIGAAIQAGVLGGEVKDVLLLDVTPLSLGIETLGGVSTRLIERNTTIPTR
                     KSQIFSTAADGQTSVEIHVLQGEREMAAGNKTLGRFILDGIPPAPRGIPQIEVTFDID
                     ANGIVNVSAKDMGTGKEQKVTITASTNLSDDEIDNAVKEAERFAEEDKKRRDEIEVRN
                     NADSLVYTTEKTIKDLEDNIDSSDKDTIQGEIDAVKKALETDDTESIRSASERLAQVS
                     QEIFGKVYQEHSQQPGADHQDQAGDHHDDNVVDADYEVVDDE"
     gene            7496..8641
                     /gene="dnaJ"
                     /locus_tag="VFD89_08505"
     CDS             7496..8641
                     /gene="dnaJ"
                     /locus_tag="VFD89_08505"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZJ58255.1"
                     /translation="MRDLAKRDYYEVLGVDKNASDEELKRAYRKLARKFHPDVNKDDP
                     QAEDKFKEINEAYGVLSDSKTRAEYDQFGHEGPTGQGFGGFDFGGFGGGFGDIFDMFF
                     GGGGFGGGGGNRRQGPTRGSDMRYNLTISFEEAAFGTTKEIEVTRNENCDECKGSGAA
                     KGTQPKTCPQCKGTGEVSYSQNTAFGRFVNVRACDKCGGEGTIVSDPCPKCNGRKRIR
                     KTKRISLNIPAGIDDGQAITLRGEGEAGERGGPAGDLYVYTTVKPHSLFSRRGYDLHC
                     DIPITFAQAALGAEIEVPSLEGRIKYKVPEGTQTGTVFRLRNQGIQRLRSTTRGDLFV
                     KVNIDVPKRLKEKQKQLLRQFDEETGGREYHEQRKSFVDKMKDVFGV"
     gene            8716..9657
                     /gene="prmA"
                     /locus_tag="VFD89_08510"
     CDS             8716..9657
                     /gene="prmA"
                     /locus_tag="VFD89_08510"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZJ58256.1"
                     /translation="MNWVELSIKTTGEGVDAVTEILSNAGINGVIIQDPRDIELLKGQ
                     KSHWDYVDEALVYGDDGVIIKGYLPDDPTLFENIRAIKDRLNWLIVQDFGIDIGPGSI
                     ELNNVREEDWANNWKQYFKPKKIGKYLVVRPGWEEYSPSEGEIILELDPGMAFGTGTH
                     ETTILCVKALEDWVAPNRNLLDIGTGTGILAIAGLLLGAKNALAVDSDSNSVRIAEKN
                     AKNNGVADRMKVVHGNLMDEVHGKFEIVVVNIIADVIIEISKEVKRYLTAGGIFIASG
                     IILDRINDVERAIREAGFTIIETKTMGEWASVVGFNE"
     gene            9650..10408
                     /locus_tag="VFD89_08515"
     CDS             9650..10408
                     /locus_tag="VFD89_08515"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZJ58257.1"
                     /translation="MNRFFIDAAQIVDSHIEIAGEDAGHILRVLRYGPGDSIELCDGA
                     GLDYPAVIVETDRDKLTVSLGEGIPSKGEPLTRITLYQGMPKSSKMDLIVQKCVELGI
                     HSIVPLNTSRTVVNLSEARRIQKRIERWQKIAEGAAKQSHRGIIPNIGKPVAFSELVA
                     SASHSIRLIPWEGETDLGLKGYLDKYGNKIYRGNGTGIGIIIGPEGGISDEEIRLAKE
                     FGWETVTLGPRILRTETAGMATLAAIMLYMGEME"
     gene            10405..11763
                     /gene="mtaB"
                     /locus_tag="VFD89_08520"
     CDS             10405..11763
                     /gene="mtaB"
                     /locus_tag="VFD89_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HZJ58258.1"
                     /translation="MKLGHGRSVAFHTLGCKVNQYDTQAMQEKFIQNGYDLTEFDDIA
                     DVYVINTCTVTNLGDRKSRQMIRRANRLNPHAVIAVVGCYAQTAPKEILNIPGVDLVL
                     GTGDRNQIVEYVEKARDMGRPIDGVHDIMKAKEFEEMAITSFEDKTRAVLKIQEGCDR
                     YCSYCIIPYARGPVRSRKPKDVIHQVKDLIDAGFREFVLTGIHVASYGKDLDGMDLLT
                     LIEEIHAIEGVKRIRLGSLEPTLLTEAFVERIKNMPKVCGHYHLSLQSGNDLTLKRMN
                     RKYTTSQFMEIVDRLRGHIADVAITTDIMVGFPGETREEFDSTMEFVKGINFSRIHVF
                     KYSPRKGTPAARYNCQISKNVKELRSRKLIALGKEMERDFNNSFIGKEEWVLFEEASK
                     NKMGWYEGYTSHYIRVAVQGDDSLIGSIGLVSLEDLEGDLVLGKLIDKAGQRSPSRQH
                     II"
BASE COUNT         3682 a         2159 c         3177 g         2880 t
ORIGIN      
        1 ggagcaggcc atgagcagac tgaaaaataa tttaaaaaat aaaacaagga gaagaatgat
       61 attcttctcc ttgttttcaa ctgctgtact tttcatatca gtattagttg tcaccaataa
      121 tactatggta cggatgacag atttaacccg tgagaaaagc cctgttttag cccaagatat
      181 gggcacatat atcacaaaga aggtcaacag ccttgctaat aatttgaggg aaatcagtgt
      241 tcatgaattt ggccaaaata tcggagatat ggtcgagaag gttattgata ttatgaaatc
      301 tttgaaattc agatggcagg gcctataagg ctataaaata gttgcttttt tgtgggttat
      361 tgattattat atggatgatt ggcttgtggg gttgggaaaa ggaagattaa aagcagttcg
      421 ctttatatcg catatggatt taggaaaatc ttttatagtg catatgccca aggcgagatg
      481 tttccataaa gcatatggta aggataatgt tacatttacg atttttaagg gggaaacaat
      541 aggcatgtcc aatgctagac aggagaagat aaggaatttt tgcataatag cgcatataga
      601 tcacggaaaa tctacgctgg cggatagaat tttagagata actgggatgc taaccgagag
      661 agagatggaa aaacaggttc ttgatgatat ggatcttgag cgggaacggg gtattaccat
      721 aaaatccaaa gccgtacgat tatcctatag ggacgaggag ggcgaagaat atatactaaa
      781 cctaattgat acgcccgggc atgtggattt tgcctatgag gtatcaagga gcttggccgc
      841 ctgtgagggg gctattttag tggtagacgc ctcccaggga attgaagcac agaccctggc
      901 caacgtatat ttggctttgg aacacgattt ggagattgta ccggttataa ataaaattga
      961 tcttcccagc gccagacctg attgggtcaa aaaggaaatt gaagacgtca taggcctgga
     1021 tgccggggat gcccctatga tctcggccaa gaacggcata ggcatagaag gcgtcgtcga
     1081 aaaaatcgtc agttctattc ctgctccaat aggggaagat gatgcgccac taaaggctct
     1141 tatatttgat tccttttatg acaactacag aggggtcatc gcctatataa gaatcaaaga
     1201 aggtaagctg aaggtaggag ataagattag gatgatggca accggaaagg aatttgaagt
     1261 tacggaagtg gggatattta ggccctcgcc gacttcggtc ccggaactta tggccgggga
     1321 ggtggggtat gttgccgcca gcattaaaaa tgtaagggat accagagtag gagacaccat
     1381 aacccactcg gacaaccctg ccgcagagcc cttgccgggg tacagaaggg taaatcccat
     1441 ggtctatagc ggagtttatc ccgcagatgg agctaggtat gaagaattgc gggaggccct
     1501 tcataagctt cagattaacg atgcctcgct gtcctttgag cctgaaacct ctatggcttt
     1561 gggctttggt tttcggtgtg gatatctggg gctgctccat atggacataa tccaggaaag
     1621 attggaaagg gagtacgacc ttgacctggt taccacagca cccagtgtta tctataaggt
     1681 gatcatgaac gacggcacaa aacaggacat atcaaaccct accagcctac cggatcctgc
     1741 ggatatcgag catatggaag aacccgtagt aaatgcccag ataatggctc caacggaata
     1801 cgtaggggcg attatggaac tgtgccagga aagacgggga atatacaagg atatggaata
     1861 catggaagaa acacgggtgc ttattaccta cgagcttcct ttgaatgaaa ttatatatga
     1921 ttttttcgat gccttaaagt ccaggaccag gggttatgca tcctttgatt atgagttgat
     1981 agggtatcag caagcagaac ttgttaaatt ggatattcta ctaaatgggg agcctgtaga
     2041 cgcgttatcc ttaatcgtcc ataatgaaaa agcctatacc aggggtaggg gcattgcgga
     2101 aaaactcaag gaacttattc cgagacaaat gtttgagata cctatccaag cggcaatagg
     2161 taacaagatt atagcccggg agaccataaa ggccctgaga aaggacgtgc tggccaaatg
     2221 ctatggcggt gatatttccc gaaagagaaa gctcctggaa aagcaaaagg aaggcaaaaa
     2281 gcggatgcgc caggtaggat cggtagaggt gccccaagag gcctttatgt cggtgctgaa
     2341 attcgattag ggataggggg atgatgtatg caggcagata caataggttt atatgttcac
     2401 tttcctttct gcttgagaaa atgtttgtac tgtgactttc catcctatgc ccacaaggat
     2461 tatctgatgg gtgaatatct gacggcttta aaagttgaga tagataatat aaaggacaag
     2521 atagggcaca gacagataaa caccgtattc ttgggtgggg gaacccccac cctttttcat
     2581 gagaaagagc ttgtttggct gcttgactat ataaagcaag gttccacaat ccgtcatgga
     2641 gccgagataa caatagaggc gaacccggaa acattaagtc cagacatgct ggaggcgctg
     2701 aagcaggggg gttttaacag gctcagtata ggtatgcagg cctgccaaga tgaacagctt
     2761 aaggcgttgg gtagaaccca tagcataggg gacgtagaac ggggggtgaa ttgggcaaaa
     2821 aaagcgggct ttcataacat aaatcttgac ctgatgttcg ggcttcccaa tcaaactacg
     2881 gaccattgga tggagtcttt ggttcgggct gtgggaatgg gtgtggagca tatttcaacc
     2941 tatgccttaa ttgtcgagga ggggacccca ttttttgaac tggcaagccg gggtggactc
     3001 aatacacctt ccgaagatgt ggaaagggag atgtatcata ggggggccaa atatttaacg
     3061 tctttgggtt ataatcacta tgaaatatcc aattttgcga aatccgggaa ggaatgcaaa
     3121 cataatttaa tatactggca caataacgag tatatcggtg tggggagcgg cgcgcattcc
     3181 tttttgcacg ggaggcgccg gtggaattat gcggatatac accaatatat cgacgaggtg
     3241 cgcaaaaatg gtgacgggac agaacaatgg gaggtgatat caccgtcaga ccagcgattt
     3301 gaaaccatta tgatgggatt acgattgaac agcggagtat caaagaaggt atttaaaaaa
     3361 cgcttcggtc atgatttggg gtattattac ggcagcatca tagagaccct caagatcgag
     3421 ggattgttaa tagaaaccga ttcaaatatt catctgacgg ctaggggcat ggatttgcaa
     3481 aacagtgtac tgcttcattt tatggaataa tattaatgaa acctattgac aaagtcttaa
     3541 tgagatgata atttaaaata agcattagca ctcattagag gagagtgcta acagatgggg
     3601 gtgatatctg tggaactagg tgaaagaaaa ttgcatatat tacaagctat tattgacgat
     3661 tatattattt cgggggaacc ggtgggatcc aggacaatag caaaaaaatc cgggatcgcc
     3721 atcagctcgg caaccatcag aaatgaaatg gcggatttgg aggagatggg ctatctggag
     3781 cagccccata cttctgccgg tcgaatcccg tccgataagg gatataggct ttatgtagac
     3841 cggctgataa aggctgaggg actcagtata aacgaagcga aatacattaa agatatatat
     3901 gacagaaaga caaggcatat tgagcaggtc atcttccaaa cctcaaagat tctatcggat
     3961 attactaact atactgctgt agtattgggt cctaaattga gccgtgtttt aattaaacat
     4021 atacaattgg tgcccataga tgcctattat gctttgctta tggtggtgac caatactgga
     4081 atagtaaagg atgctatcat cagcatacct caaggtgtcg gcatggatta tctggccagg
     4141 atatcaaaca tactaaatga cctatttaaa gaccagagtc ttggagagat cagtctggag
     4201 ccgttgatag aaatgcggga aggaatgatc caaaataggg atttttttaa ttccctagtg
     4261 gatgctctca ccgagagtat agatataggc gatataagtg aagtttattt gggcgggatg
     4321 accaatatat tcaactaccc tgagtaccat gatattttta aagccaaaga atttttaaac
     4381 cttatggagc aaaaggagct tctgtatagg ctcttggcaa gatcccagga taacggcgtc
     4441 tccgttacca ttggggagga gaatcaatgc gaagaactcc gcgagtatag tataattacc
     4501 gcaacctaca atattaatgg taagactttg ggcactatgg gtattattgg tcccacacgg
     4561 atggagtact ccaagactat atccgttatg gattttatgg ggaagaccct cagtaactat
     4621 ctaacccgac tattttcgaa ggaatagtaa atataaaaat ggcatgtcta aattagaggt
     4681 aagggcccgt ataggacctg ttttatctat ggttgtcata aggatatgcc cctgagacat
     4741 gttaacttag ctgaataagg tgtagaaatg gagaaggtta tatggggaaa gataagagag
     4801 aaaagaacac ggaggatatt ctagggatgg attatggtca agaagacaga gcagaagaaa
     4861 taaccccgga tggggggtca gagtgtacgg gagaagccaa agagctgtgg ctcctggaag
     4921 aacagctgca aaaggccgga gaggaaaggg atatgtacct ttctatggcg caaaggcttc
     4981 aggccgagtt tgataatttt agaaaacgca atagggcggc tgtttctgcg gcccaccagg
     5041 actcggtcta tgagactgtg gaactttttt tgcccatttt agataacttg caaaggggcc
     5101 ttgaatcccc ggggaataat gaatccaccc aggtgcacgc tatatataag ggtgttgaga
     5161 tggtcaccaa acagtttaca gacatattgg aaaaattggg tatagaagag attgaggctc
     5221 tggatcaacc ctttgatcct acataccaca atgctgtcat gcaggtagag gcagagtatg
     5281 aagaacagaa gaacaaggtt gtcgaagtcc tgttaaaggg atacaagggt aaagataggg
     5341 taataagata tagtatggtt aaagttgcca tttgagcgtt aggtgctctc catgctttac
     5401 gacaagcgtt ctacatttaa attaatattt aacaactttg ggcatccgcc ggaagacaag
     5461 gaatataaaa tccgcgtaca gttttgtgta tgttcgggct gcattgtgca cacgacataa
     5521 accggatgat agatatatca cctttttcgg ggaggccgta ttattaggag gaaaataatt
     5581 atggggaaga taataggaat tgatttagga acaacaaatt catgcgtagc agttatggaa
     5641 ggcggggaag cagttgtaat ccccagcgct gaagggggca gaaccatgcc gtctgtagta
     5701 gccttttcca aaaccggtga aaggctcata gggcaggtag ccaagagaca ggcgattact
     5761 aatccggaac atacgattat gtcgattaaa agggatatgg ggagcgatcg aaaggttaga
     5821 atcgaggaca aaacatatag tccccaagag atttcagcca tggtcttaac gaaaatgaag
     5881 caggatgccg agagctattt gggggaaaaa gtgacccagg ctgttattac agttcctgca
     5941 tattttagtg acagccagcg ccaagctacc aaggatgcag gagtaatagc ggggttggat
     6001 gtactcagga taataaacga gcccactgca gcagcattgg cttacggatt ggacaaggaa
     6061 gataatcaaa agatcttagt atatgacctg ggcggcggca cttttgatgt ctccattatg
     6121 gagataggcg acggtgtttt tgaggtcttg gcaaccaggg gaaacaatag attgggcggg
     6181 gacgattttg accagaggat aattgattac cttgccgatg agtttaaaaa atccgacggt
     6241 atcgatctta gaatggataa gatggcctta cagcgtctca aagaggctgc cgaaaaggct
     6301 aagatagagc tatcaagcgt tctcacaacc aatgtcaacc taccctttat tacggcagac
     6361 gcgtccgggc ctaagcattt ggatgtgact ctcacaaggg ctaagttcga ggagatgacc
     6421 gccgatctag tggaaaaaag cctgtccccc cttcgggcgg caataaaaga tgccggactg
     6481 tcagccaatg agattgacag ggttatattg gtagggggct ccataaggat tcctgcggtc
     6541 caggaggctg taaaaaaggt gacggggaag gatccccata agggaataaa ccccgacgaa
     6601 tgcgtagcta tcggggctgc catccaagcc ggagtcttag gcggtgaagt aaaggatgtc
     6661 ctcttattgg atgtaacgcc gttatctttg ggaattgaga ccctcggcgg ggtttcaacc
     6721 agattgattg aaagaaacac caccataccg actcgaaaaa gtcaaatctt ttctaccgct
     6781 gcggatggac agaccagcgt agagatccat gtgctgcaag gagagcggga aatggcggca
     6841 ggcaataaaa ccttaggtag atttatactg gacggcattc cgcctgcacc caggggtata
     6901 ccgcagattg aagtaacctt tgatatagat gccaatggta tcgttaacgt atccgccaag
     6961 gatatgggaa ccggtaaaga acaaaaggtt actattaccg catctaccaa tctgtccgat
     7021 gacgagatag ataatgcagt gaaagaagcc gaaagattcg cagaagagga taaaaagcgt
     7081 agggatgaaa tagaagtaag aaataatgcg gattctcttg tctataccac tgaaaagaca
     7141 atcaaggatt tagaagataa tatagattcc tccgataagg ataccatcca aggtgaaata
     7201 gacgccgtga aaaaggccct agaaaccgac gatacagaaa gtattaggtc tgcatctgaa
     7261 agattagccc aagtttctca ggagatattt ggcaaggtat atcaagagca tagccagcag
     7321 ccgggagcgg atcaccaaga tcaagcaggg gatcaccatg atgataatgt tgttgatgca
     7381 gattatgaag tggtagacga tgaataaaaa ataaggtata attgtttcaa aaagccaaag
     7441 ccataaaggc tttggctttt tagaagaaat aaaatctgag caataaagca aggtgatgag
     7501 ggacttggca aagagagatt attacgaagt tttgggtgtg gataaaaacg catcggatga
     7561 ggagttaaaa cgggcgtaca gaaagcttgc aagaaagttt catccggatg tcaataaaga
     7621 cgacccccag gctgaagata aatttaagga aataaacgag gcctatggtg tattaagtga
     7681 ttctaaaacc agggcagagt acgatcaatt cgggcatgag ggtcctaccg gtcaggggtt
     7741 tggcggattc gatttcggcg gattcggtgg gggctttggc gatatatttg atatgttctt
     7801 cggcggcgga ggttttggcg gcgggggcgg caatcggaga caggggccta caaggggttc
     7861 tgatatgaga tataacctta ctatttcctt tgaagaggca gcatttggca cgacaaagga
     7921 gattgaagta accagaaatg aaaattgtga tgaatgcaag ggcagtgggg ctgctaaggg
     7981 aactcagcct aaaacctgtc ctcaatgcaa gggcaccggc gaggtttcgt attcccaaaa
     8041 caccgccttt ggacgttttg taaatgtcag ggcatgcgat aaatgcggcg gtgaagggac
     8101 catcgtatcc gatccctgcc ctaaatgtaa tgggaggaaa agaattagaa agacgaagag
     8161 gatcagcctt aatattcctg cggggattga cgacggtcag gctattaccc tccgcggcga
     8221 aggcgaggcg ggagagagag ggggtcctgc gggggatctc tacgtgtata caaccgtaaa
     8281 accccatagt ctattctcaa ggagaggata cgaccttcat tgcgatatac cgatcacctt
     8341 tgcacaggcc gcgttaggtg cggaaatcga ggttccctcc ttggaaggca ggattaaata
     8401 taaggtaccg gagggtacgc agacgggaac tgttttcaga ctccgaaacc aaggaataca
     8461 gcgcctgcgt tcaacgacca ggggtgacct ttttgtaaag gtgaatatag atgtccctaa
     8521 aaggcttaaa gaaaagcaga aacagctcct gagacaattc gatgaggaga ccgggggaag
     8581 ggaatatcac gaacagagaa agtccttcgt tgataagatg aaggatgttt ttggagtata
     8641 aatgggagca ttcgaaggga ggaggacggg cgtttaatct cgtgattccg gtcacaagag
     8701 tttatgtcca tatttatgaa ttgggttgaa ttatcaataa agacaaccgg tgaaggtgtc
     8761 gatgccgtca cagagatcct gtctaatgcg gggataaatg gcgttataat ccaagatccc
     8821 cgtgatatcg aactgcttaa gggtcaaaaa tcccattggg attatgttga tgaggctctg
     8881 gtctatggcg atgacggagt gattattaag ggttatttac ccgatgaccc gacattattc
     8941 gaaaacattc gagctattaa ggacaggctc aattggttga tagtgcaaga cttcggcatt
     9001 gatataggac cgggcagcat cgaactcaat aatgtacggg aagaggactg ggctaataat
     9061 tggaaacaat atttcaaacc aaaaaaaatc gggaaatacc tggtggtgag gcccggctgg
     9121 gaagaatatt ctccgtcgga aggggagatt atcctggaat tggacccggg tatggccttt
     9181 ggaacgggca cccacgaaac caccattcta tgcgtcaagg ccttggaaga ttgggtagcc
     9241 cctaatagaa acctattgga catagggacc ggaactggga tcctggccat agccggtctt
     9301 ctattgggtg caaaaaatgc cttggctgta gactcggatt caaattcggt gagaatagcc
     9361 gaaaaaaatg ctaaaaataa tggggtagcc gacagaatga aagtagtaca cggcaacctt
     9421 atggatgagg tccatgggaa gttcgagata gtggtcgtca atatcatcgc agatgtaata
     9481 atagaaatat ccaaagaggt aaagagatat ttaaccgccg gcggcatctt tatagcctcg
     9541 gggataatac tggaccggat aaatgatgtt gaaagggcaa tcagagaagc ggggtttacc
     9601 atcatagaaa caaaaactat gggagaatgg gcatcggtgg tgggttttaa tgaataggtt
     9661 cttcatagat gccgcccaaa tagtcgattc tcatatagag atagccggtg aggatgcagg
     9721 gcatattctt agggtcttaa gatacgggcc cggcgactcc attgagcttt gtgatggggc
     9781 cggactggat tatcccgccg tcattgttga aaccgacagg gataagctga cggtaagtct
     9841 tggggaaggt atcccgtcaa agggtgagcc tttaacaagg atcaccctat accagggaat
     9901 gcctaaaagc tccaaaatgg accttatagt ccaaaagtgc gtggagcttg gaatccacag
     9961 catcgttccg ctgaatacat cgagaaccgt agtaaacctg tccgaagcga gaaggattca
    10021 aaaaaggatt gaaagatggc aaaagatcgc tgagggagct gccaagcaga gccatagggg
    10081 cataattccc aacataggga aacctgtggc attttcagaa cttgtagcct cagcctcaca
    10141 ttcgattcgg ctcataccct gggaggggga gacagatctt ggtctgaagg gctatttaga
    10201 taagtatggg aataaaatat ataggggtaa tgggacgggg ataggaataa tcattggtcc
    10261 cgaaggcgga atcagtgatg aagagataag gctggctaag gaatttggct gggaaaccgt
    10321 tactttaggc cccaggatcc ttaggactga aactgcaggg atggcgacct tggcggcaat
    10381 aatgctatat atgggggaaa tggaatgaaa ttagggcatg gtagatcagt agctttccat
    10441 accttgggat gtaaagtcaa ccaatacgat acccaagcca tgcaggaaaa gtttattcaa
    10501 aatggttatg atctgaccga attcgatgac attgctgatg tatatgtaat caacacctgt
    10561 actgtaacca atctgggaga cagaaaatca agacaaatga tccgtagggc caatcgatta
    10621 aatccccacg ccgttatcgc tgtggtggga tgctatgccc agaccgcacc gaaggagata
    10681 ttgaatattc ccggggtgga tctggtgttg ggcacggggg atagaaatca gatagtcgag
    10741 tacgttgaaa aggcccggga tatgggcaga cccattgacg gtgttcatga tattatgaag
    10801 gcaaaagaat tcgaggagat ggccataacc tcctttgaag ataagactag ggccgtgctt
    10861 aagatccagg aggggtgtga tcgctattgc tcatactgca ttatacccta cgctaggggt
    10921 cctgtcagaa gccggaagcc gaaagatgtg atacaccagg taaaagacct tattgatgca
    10981 gggtttcggg aattcgtttt gaccgggatc catgttgcat cctatggtaa ggatctggat
    11041 gggatggacc tgttaacctt gattgaagaa attcacgcta tagagggtgt taaaaggatc
    11101 agattgggct cattggaacc taccctttta acagaggcct ttgtggaaag gataaagaat
    11161 atgcccaagg tttgcgggca ttatcacttg tccttgcaaa gcggtaatga ccttaccttg
    11221 aagaggatga accgaaagta cacgacctcc caatttatgg agatagttga caggctcagg
    11281 ggacacatag ccgatgtagc cataacaacg gacatcatgg taggattccc cggtgaaacc
    11341 cgggaagaat ttgatagcac catggagttt gtaaaaggaa taaattttag ccggattcat
    11401 gtttttaaat attcccccag aaagggaaca cctgcagcaa gatataactg tcagatttca
    11461 aagaatgtaa aagagctccg gagtcgtaaa ttgatagctc tgggaaagga aatggaaagg
    11521 gattttaata attcctttat aggcaaggaa gaatgggtct tattcgaaga ggccagcaag
    11581 aataaaatgg gttggtacga gggctatacc agtcactata tcagagttgc cgttcagggg
    11641 gacgattccc ttataggctc cataggattg gtaagcctag aggatttaga aggtgatttg
    11701 gttctgggaa aattgatcga taaggccgga cagaggtccc catccaggca acatatcatt
    11761 tgaaccttcg aacatatcct ttgaaccttc aattgatcaa ttatttattt ttcgatgata
    11821 cccaaaccaa aagaaggatt ttgggacatt gatgtagaat atattgtttg gaagggggtg
    11881 ttttatatgc cggataac
//
LOCUS       DASRNX010000217         4246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_94895,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000217 DASRNX010000000
VERSION     DASRNX010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94895"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..576)
                     /locus_tag="VFD89_08525"
     CDS             complement(190..576)
                     /locus_tag="VFD89_08525"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZJ58259.1"
                     /translation="MHQRLMNEAIRVKDMAYAPYSDFRVGAAVLAGDKIYTGCNIENI
                     SYGATCCAERVAIFKAISEGIRDFKAIAVASDSSGPTFPCGICLQVMAEFNVPKIVVS
                     DGGGGYNEYTLDDLLPWAFDDISRSL"
     gene            complement(586..1299)
                     /locus_tag="VFD89_08530"
     CDS             complement(586..1299)
                     /locus_tag="VFD89_08530"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HZJ58260.1"
                     /translation="MSKLIKRRSLVESFNDAIEGLFHAFRSERNMKIHLIIALIVLSV
                     AVFFQVSRIEIILLFISITLVVVAEMINTAIEIIVDMIKEDYHPMAKIIKNVAAGGVL
                     ITAINAVVIGYILFYDKIDKLSLSIIKQIKTMPVHITAASLIMVAISVIIIKNINKTG
                     TFLKGGMPSGHSALAFSLFTCITLISANALLSTLGILMAFMVLHSRYETGIHTMYEIM
                     VGAIIGVILTVLAFQIAKF"
     gene            complement(1383..1916)
                     /gene="ybeY"
                     /locus_tag="VFD89_08535"
     CDS             complement(1383..1916)
                     /gene="ybeY"
                     /locus_tag="VFD89_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZJ58261.1"
                     /translation="MAKQTMDMHDGITVEIDNDQDVIELTDGMEDIIVGCIHRTLDAG
                     GFGFDATVGITLVDNRSIEEMNLQFRGIAAATDVLSFPMLEIQAGCMDLDEEEFINDV
                     HPETGSVILGDIVISMEKMREQALEYGHSFERELGFLVVHGMLHLLGYDHVEEADMNI
                     MRSKEEAILKSLSLVRF"
     gene            complement(1888..4134)
                     /locus_tag="VFD89_08540"
     CDS             complement(1888..4134)
                     /locus_tag="VFD89_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HZJ58262.1"
                     /translation="MMVNQIVTRKGDAPPARGFIGSLRSAKVLGALLAVVNYIFIFIV
                     LMTSVLPKQYSLKVGDIVQTPIGAPWDVEDIHATEELRQTAKDSVSPIYRFDERITES
                     VMEEIEDIFVKIDGARILAEEKFIEYKLELQAQQTNNDQIEEGQEEEAHETGEDIAEP
                     SPDEILTDQFISEIRNDFPIMLSKDEILTCLNADIDELHQLREHLLTTLNGLLELHIK
                     QDNLLEAKNALREEMMSLPISNEIRILGTSIGVSALRSNMLPDETATEEERVRAAENV
                     EPVMYKKGQYIVQAGQPISHQQYAMLQKLGLLKEEKMNVPILLGIGIIILLIELMVIL
                     FLAAFEKRLLDNPAHLLMMSLIVWMVMGLAIATSQLHQYLIPTALGAMLLTILTGPFA
                     AGIINVALSLLFGVMFENSLSIIIMTLVGGIVGIYICNNAKQRNGLIWAGIAASIVNL
                     ASIFALEMMSSGGWFTSLKTSLWGIGGGLLSGIFLVGTLPIWENLFGIVTPIKLVELA
                     NPNHPILKRMLMEAPGTYHHSVIVANLAENAAEAIGADGLLARVGAYYHDTGKLKRPF
                     YFKENQIAMGNPHDKLSPTLSTHIITSHVKDGIDLAKKHKVPKVIQDFILQHHGTTAV
                     AYFYHKAKEQDEDGSVHMDDFRYAGPKPRKRETAIVMMADTVEAAVRSISDPTPEKVE
                     ALIHRLVKSKLDDGQLDDCHLTLKDLNTISNAFTGVMTGFFHERVEYPKTSEKQESIG
                     GETDNGYA"
BASE COUNT         1169 a         1034 c          801 g         1242 t
ORIGIN      
        1 gagtagactt acccacattg gaccgcccta ttactgcgac aaaaccagat ctgaatccct
       61 tatccatttt atcatccttt aaatttagca tgtattaaaa acaattaaag cattttccct
      121 atttggaaaa ttatacgaaa atcacattgt gtaaaggctt agaacctatt gcacagcgaa
      181 gggccgaccc tataacgatc ggcttatatc gtcaaaggcc caaggcaaaa gatcgtctaa
      241 ggtatattca ttatatcctc cgccaccgtc acttacgaca attttcggaa cattgaattc
      301 agccataacc tgtagacaaa taccgcaggg aaaggtaggg ccggaagaat ccgatgccac
      361 cgcaattgcc ttaaaatccc taatcccttc agaaatggct ttgaaaatgg ctaccctttc
      421 agcgcagcag gtggccccgt agctgatatt ctctatattg cacccggtat atattttatc
      481 accggctagc actgcagcac ctaccctaaa gtccgaatag ggcgcataag ccatgtcttt
      541 aacccttatt gcttcgttca taagcctttg atgcatacct acctcctaga acttggctat
      601 ttgaaacgcc aaaaccgtca gtattacccc tataattgca cccaccataa tctcgtacat
      661 ggtgtgtata cctgtctcat aacgactatg gagcaccata aaggccatca atatccccaa
      721 ggtagataat aacgcattag cacttataag ggtaatacag gtaaataatg agaaagctaa
      781 tgcgctatgt ccgctgggca taccaccctt taggaaggtt cccgttttat tgatattctt
      841 gataatgatt acggatattg ccaccattat caaactggcc gctgtaatat gcaccggcat
      901 agtcttaatt tgttttataa tggataggga cagtttatct atcttatcgt agaataatat
      961 ataccctatc actacggcgt tgatggccgt gatcaacacc cctccggccg caacattctt
     1021 tataatcttt gccatggggt ggtagtcctc tttaatcata tccacaatga tttcgatggc
     1081 tgtattgatc atttcagcca ccaccacaag ggtaatggat atgaacaata aaatgatctc
     1141 tatcctagat acctgaaaaa atacggccac ggagagtaca atgagggcta taatgaggtg
     1201 tatcttcatg tttctttcgg atctaaaggc atggaacaaa ccctcaatag catcattgaa
     1261 actttctact aggcttctac gcttaattaa cttggacata aaaacccccg attcaaaagg
     1321 taaggcaaat gctgatcctc ctttttagat ttactgcttt ataatcgaga gacttaacaa
     1381 tcttaaaacc tgaccaggct tagggatttt agtatagcct cttctttgga acgcattata
     1441 ttcatgtccg cctcctctac gtggtcatag cccaatagat gcagcattcc atgtactact
     1501 aaaaaaccca gctcccgttc aaaactatgg ccatattcaa gtgcctgctc ccgcatcttt
     1561 tccatggata taacaatatc ccccagaatt acggagccgg tctcgggatg gacatcgtta
     1621 ataaattctt cctcgtccaa atccatacag ccggcctgta tttccagcat gggaaaggaa
     1681 agtacatcgg tagccgcggc tatacccctg aattgtaaat tcatctcttc tatggatcta
     1741 ttgtccacaa gggtaatgcc caccgtggcg tcaaagccga agcccccagc atctaacgtc
     1801 ctgtgaatac agcctacgat gatatcttcc ataccgtctg taagttcaat tacatcctga
     1861 tcattatcga tctccacggt tattccgtca tgcatatcca ttgtctgttt cgccacctat
     1921 tgactcttgt ttttctgatg tttttggata ctctactctt tcatgaaaaa atccggtcat
     1981 cacaccggta aaggcgtttg aaatagtatt gagatccttg agggtcagat gacaatcgtc
     2041 taattgtcca tcgtccagct tgctcttgac aagcctgtgt atcaaggctt ctaccttctc
     2101 aggtgtcggg tcggaaatag accttacagc agcctcaacc gtatccgcca tcataactat
     2161 ggccgtctcc ctcttcctag gcttagggcc tgcgtacctg aaatcgtcca tatggacact
     2221 cccatcctcg tcctgttcct tggccttatg ataaaaatag gccacagcgg ttgtgccatg
     2281 gtgctgtaaa ataaagtctt ggattacctt gggaacctta tgctttttgg ccagatctat
     2341 gccatccttt acatgggagg tgataatatg ggtgcttaga gtcggactca atttatcatg
     2401 gggatttccc atagctatct ggttttcctt aaaataaaag ggtcttttta atttacctgt
     2461 atcatgataa taggcaccta cccgggcaag gagcccatcg gcacctatag cctctgccgc
     2521 attttcggca aggttggcca caataacact atgatgatac gttcccggtg cctccatcag
     2581 catgcgtttt aatatggggt ggttgggatt tgccaattct accagcttta tgggggtcac
     2641 gatcccaaac aaattttccc aaataggcaa tgtccccact aaaaaaattc cggacaataa
     2701 tcctccgcct ataccccaaa gggatgtttt aagggaagtg aaccagccac cggaggacat
     2761 catctctaag gcaaaaatac ttgccaggtt tacgatgcta gccgctatcc cggcccatat
     2821 aagtccattg cgttgcttgg cattgttgca tatataaatg cccacaatgc ccccaactag
     2881 ggtcataata ataatgctta gtgaattttc aaacattacg ccaaatagta gggacagtgc
     2941 aacattgatg atcccggcag caaatggacc tgttagaata gtcagcaaca tggcccccaa
     3001 tgcagtggga ataagatact ggtgaagctg gctggttgct attgcaagac ccataaccat
     3061 ccacactata agactcatca tcaaaagatg agcgggatta tctaggagtc gcttttcaaa
     3121 ggccgccaga aataggatta ccattagttc tatcaataga attattatac ctatgcccaa
     3181 cagaatgggg acattcatct tctcttcctt taacaggccc aatttctgta gcatcgcata
     3241 ttgctgatgg ctgatgggtt gtcccgcttg gactatatat tgaccttttt tgtacataac
     3301 gggttcaacg ttttcagccg ctcggaccct ctcctcttcc gtcgcagttt catcggggag
     3361 catattggat ctgagggctg atacgcctat gctggtgccc aagatcctaa tctcattaga
     3421 gataggcagg gacatcatct cttccctcag ggcatttttt gcttccaata aattgtcctg
     3481 cttgatatgc agttccaaca gtccgtttaa tgttgtcagt agatgctcac gtagttgatg
     3541 gagctcatca atatcggcat tgagacaggt taaaatttca tctttggaga gcataatagg
     3601 aaaatcattt cttatttcgc ttatgaactg atctgttaga atctcatcag gactcggttc
     3661 ggctatatct tccccagttt catgggcttc ttcttcctgg ccctcctcaa tttggtcatt
     3721 attcgtctgc tgagcttgta attccagttt atattctata aacttctctt ctgctaatat
     3781 ccttgcccca tctattttta caaatatatc ctcaatttct tccataacgc tttcggttat
     3841 gcgttcatca aacctatata taggggacac actgtccttt gcagtctgtc ggagctcttc
     3901 agtagcgtgt atatcctcaa catcccatgg tgcgcctatg ggggtttgca ctatgtcccc
     3961 caccttcaaa gaatattgct tgggtaaaac tgaagtcatg agcacaatga agataaatat
     4021 ataattaaca acggctaaca aagcccctaa aaccttcgca cttcttaaag aacctataaa
     4081 tccccttgcg ggtggggcat ctcccttcct ggtaacgatc tggttcacca tcatctctcc
     4141 tcttcccgaa tagaagaatc cacacatcaa gcgttatatt attctagtag tcgaagaatc
     4201 tacacatcaa gcgttatatt attctagtag tcgaagaatc tacaca
//
LOCUS       DASRNX010000218         8075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_309453,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000218 DASRNX010000000
VERSION     DASRNX010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8075
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309453"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2356
                     /locus_tag="VFD89_08545"
     CDS             <1..2356
                     /locus_tag="VFD89_08545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HZJ58263.1"
                     /translation="YREGLIIGSGCEAGELFRAMVNGSPYNEILEIANFYDFLEIQPM
                     GNNEYLVRDKHLSSHKDLEDINKNIVKLGEKIGKPVVATGDVHFLDPQDEYFRRILMN
                     GQGFNDADFQPPLYLKTTAEMLEDFKYLGEEIAKEVVIEAPRRISTSIESIEPVPQGL
                     STPEIPGAEDQIREMAIDNALKIYGNPLPEIVGSRLEKELKSIIEHGFAVLYLIAHKL
                     VQKSLTDGYLVGSRGSVGSSFVAYLTNITEVNPLSPHYICSQCKHSDFHIDKARYGVG
                     IDLPDKGCPNCGTAYRRDGFDIPFEVFLGFDGDKVPDIDLNFSGEYQPKAHKYTEELF
                     GEGHVFRAGTIGTIAEKTAFGFVKKYLDERSKVVSNAEIKRLVAGCTGVKRTTGQHPG
                     GIMVVPKGREIYEFTPIQYPADAKDSGVITTHFDYNFIHDNLVKLDILGHDDPTMIRM
                     LEDITGIDSTQISLDDEGTMAIFSGTAILGIKPRELGSQVGTFGIPEFGTRFVRQMLV
                     DTKPTTFSELIRISGLSHGTDVWLNNAQGLIRDGVAQLSEVISTRDDIMIYLIYKGLE
                     PTMAFQIMENVRRGRGLTDEFENAMVAKNVPNWFISSCKKIKYMFPKAHASAYVIMAF
                     RIAYFKVHYPSAFYAAYFTIKATDFDADFALGSEDIIREKIRELESKGNSGTAKERSL
                     LIILEVILEMYLRNIFFLPVDLYRSHPIKFLIAEDGIRPPLNTLQGVGANAALSIEQA
                     RTEGEFISLEDLRERTKITKTAVEILRKHGALDGIPETSQLSLF"
     gene            2607..3068
                     /gene="rimP"
                     /locus_tag="VFD89_08550"
     CDS             2607..3068
                     /gene="rimP"
                     /locus_tag="VFD89_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZJ58264.1"
                     /translation="MSKKSVEEIAREIALPIIEELDYELVDLEYKKEGPHWCLRLYID
                     KPGGITIDDCQEASKRVSDALDLKDPIPHNYFLEVSSVGLDRPLKKEEDFVRFKGHKV
                     DIRLYRAIDGSKSFTGDLIGLVEGAIRVQVGEKAIDFERDQVAIIKLSVEF"
     gene            3132..4199
                     /gene="nusA"
                     /locus_tag="VFD89_08555"
     CDS             3132..4199
                     /gene="nusA"
                     /locus_tag="VFD89_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZJ58265.1"
                     /translation="MNGEFLGALDQIASEKGIDKEILVDAIEAALQSAYKKNFGTSQN
                     VKVNVDKLTGVVKVYAMKTIVEEVMDESQQIFLEDAQRIDPVFSLGDIVEVEVTPKNF
                     GRIAAQNAKQVVVQRIREAERALIYEKFIEKENEIMTCVVQRAERRNVFVDIDGAEGT
                     MPPNEQISNESYRVNDRLMVYITEVKMTNKGPQITVSRTHPSLVKRLFEREVPEIIRG
                     EVIIKDIAREAGSRTKIAVFTHHPSIDPIGACVGQHGIRVQHIVDELRGEKIDIIKWS
                     DDPREYIGSALSPARIVNVQIHEVEKSARVIVPDNQLSLAIGKEGQNVRLAAKLTGWK
                     IDIRSISQMESIDSTQEGNLD"
     gene            4214..4489
                     /locus_tag="VFD89_08560"
     CDS             4214..4489
                     /locus_tag="VFD89_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HZJ58266.1"
                     /translation="MKKRKIPLRMCVGCNEIRPKGELIRVVRSPQGDIYIDQVGKRPG
                     RGAYICKNLDCLENALKSRKLERAFGQKIDQEIYQRLSIEMGEGENN"
     gene            4476..4814
                     /locus_tag="VFD89_08565"
     CDS             4476..4814
                     /locus_tag="VFD89_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016284157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HZJ58267.1"
                     /translation="MRIINDKVYQLVGLSKRAGKLVSGSHAVENAIKSGKAQLVIVSE
                     EASANTVKKFFDMCEFRNVNIIKIGSKKDLGECIGKTDRTLVAITDVAFRDMIVEVLP
                     SPITNTGVID"
     gene            4814..7126
                     /gene="infB"
                     /locus_tag="VFD89_08570"
     CDS             4814..7126
                     /gene="infB"
                     /locus_tag="VFD89_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZJ58268.1"
                     /translation="MSKINVYSTAKKVTEGARELMDMVGETEKGIAGYKNTILKLRER
                     LNRETIERELEEKRKEQRLEEESRRKKEQQIRAEERAKRESGPAAPGRTGEKRPMGPG
                     TRDLQNRLKEKSGESIRVIDSTGGKPGQDKVVPSKGYENTQKRRERRSKKDFWQEGES
                     YSRIKKLRETKTTSQKRQEKSGKVPERKKAIIMGELITVKDLSEKIGVSVPEIMKKLM
                     GLGILATINQDLDYDTAAIISAEFGIQLDKRVIKSFEEILEDEDLEDDPDSLKPRSPI
                     VTVMGHVDHGKTSLLDAIRSSRVTAQEAGGITQHIGAYTVNVDNRLITFLDTPGHEAF
                     TSMRARGAQVTDIAILVVAANDGVMPQTVEAINHAKEAGVPIIVAINKIDVTGANPER
                     VKQELTEHELLVEEWGGDTIAVPVSALRNEGIDSLLEMVLLVAEMQELKANPNRLAKG
                     TIIEAELDKGRGPVATVLVQKGTLRVGDSMVAGTAYGRVRAMMDHTGKRLEKAGPSTP
                     VQVLGLTEVPEAGDTMYCVEGDKLAKQVSEERKEKVRELQIRASSKVSLDELFNKIKE
                     GEVKDLNLIIKADVQGSAEAVKQSLERLTNEDVRIRTIHGGVGAITESDVMLATASNA
                     IIIGFNVRPSPGASELASKEEIDIRLYRVIYNAIEDIEAAMRGMLDPEYKEVILGHAQ
                     VRETFKVPGAGIVAGSYVTDGKILRNADARIVRNGIVIYEGKISSLRRFKDDVREVTA
                     GYECGISLVNFNDIKEDDVIEVSIQEEIKR"
     gene            7154..7525
                     /gene="rbfA"
                     /locus_tag="VFD89_08575"
     CDS             7154..7525
                     /gene="rbfA"
                     /locus_tag="VFD89_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZJ58269.1"
                     /translation="MKHQRIDRISEEIKKEISRIIREDVKDPRIGQMASILRVDVSGD
                     LRHAKVYVSVLGDERERASTIAGLSRAAGFIRKGLGRVLTIRYIPELNFILDTSIEYS
                     VDISKKIMEIKKQQEQSNGDD"
     gene            7512..>8075
                     /locus_tag="VFD89_08580"
     CDS             7512..>8075
                     /locus_tag="VFD89_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HZJ58270.1"
                     /translation="MGMIDIIQPIDRSDSIALITHMNPDGDAVGSLIALMRALDKMGK
                     SVDAYCQDEVPGALNFLDGVARITRPIDPIKRYDLAIALDCSDRGRMGDCATIMDMAT
                     SSANIDHHISNTFYADINIVDGGASATGEMIYELVELLIDSADKGIAEALYTAIVSDT
                     GGFAFSNTTAKTHRTAANLIEWGAETAK"
BASE COUNT         2564 a         1394 c         2151 g         1966 t
ORIGIN      
        1 gtatagggaa ggcttaataa tagggtccgg gtgtgaagca ggtgaattgt ttagggccat
       61 ggtgaatggt agtccttata atgaaatctt ggaaatagcc aacttttatg acttcctaga
      121 gattcaacct atgggaaata acgagtattt agtacgggat aagcatctaa gtagtcataa
      181 ggacctagag gatataaaca aaaatatcgt aaagctggga gagaagatag gaaaacctgt
      241 ggtggcaaca ggagatgtgc attttttaga tccccaggat gaatatttta ggagaatatt
      301 gatgaatgga caggggttta atgatgccga ttttcagcca cccctatacc taaagacaac
      361 agccgaaatg ttagaggatt ttaaatactt gggcgaggag atagccaaag aagttgtaat
      421 tgaggcacct aggcgtattt cgacctcaat tgaaagtata gaacctgtgc cccagggtct
      481 atccacacct gagatacccg gtgccgaaga tcagattagg gagatggcga ttgataatgc
      541 actaaaaatt tatggcaatc cactaccaga gattgtaggg tctcgattgg aaaaggagct
      601 aaaatccatc attgaacatg gttttgcggt tctatatttg attgcgcata agctcgtgca
      661 aaaatcttta acagatgggt atcttgttgg ctctaggggt tctgtgggtt catcctttgt
      721 agcatatttg acgaatataa cggaggtcaa tcctctttcc ccccattata tttgcagcca
      781 atgtaaacat agcgattttc atattgataa agcacgatat ggtgtgggta tagatctccc
      841 cgataaaggc tgtccaaatt gtggcacggc atatagaagg gatggatttg atataccctt
      901 tgaggtattt cttggctttg acggcgataa ggttcccgat atcgacttga atttctccgg
      961 tgaatatcaa ccaaaagcac acaaatatac agaggaatta tttggggaag gacatgtctt
     1021 cagggcagga actatcggaa ccatagcaga aaagacagcc tttggtttcg taaaaaagta
     1081 tttggatgag aggagcaagg tggtcagcaa cgcggagata aaaaggctgg ttgcggggtg
     1141 caccggtgtg aaacgaacaa caggacagca tccggggggc attatggtgg tccctaaagg
     1201 gcgtgaaatt tatgagttta cgcccataca gtacccagca gacgcaaagg actccggtgt
     1261 catcaccacc cattttgatt ataattttat ccacgataat ttggtgaaac tagatatatt
     1321 aggccatgat gaccccacca tgattaggat gctggaggat attacgggga tagattcaac
     1381 gcagatctca cttgatgatg aagggaccat ggcgattttt tccggaactg caatccttgg
     1441 cataaaaccg agggagttag ggagccaagt tggtacattt ggaataccgg aatttgggac
     1501 ccgattcgtc agacagatgc tggtggatac taagccgacc acctttagcg agcttatacg
     1561 tataagcggg ctatcccatg gaacggatgt atggctcaat aatgcacaag gcttaataag
     1621 ggacggggtg gcgcagctat cagaggttat atctacaagg gacgatataa tgatatactt
     1681 gatatataaa ggccttgagc cgactatggc gttccagatt atggaaaacg ttcgaagggg
     1741 aaggggtctg accgatgaat tcgaaaatgc aatggtggca aagaacgtac ccaattggtt
     1801 tatatcatcc tgtaaaaaga taaagtacat gttcccgaag gcccatgcct ctgcttatgt
     1861 gataatggcc tttaggattg cttacttcaa agtccattac ccaagcgcct tttatgcggc
     1921 atattttacc attaaagcta ctgactttga tgccgatttc gctttgggta gcgaggatat
     1981 tattagggaa aagattaggg aattggaatc caagggcaac agcggaacgg caaaagaaag
     2041 aagcctattg atcattttag aagtcatatt ggagatgtat ttaaggaaca ttttcttctt
     2101 gcctgtggat ctgtacaggt cccatcccat aaaatttcta attgccgaag atggcattag
     2161 gccgccttta aatacgctgc agggagttgg tgcaaatgca gcgttgagta ttgagcaggc
     2221 aagaacggag ggggaattta tatcattgga ggacctgagg gaaaggacta agattaccaa
     2281 aactgccgta gaaatcctaa ggaaacacgg ggcattagac ggcattccgg agacaagcca
     2341 actttccctg ttttaagtct tgcactacta tatatttgat gctataattg gttttaggcc
     2401 aaatagtatc ggtttaagac agagtgggga ataacccact ctttcatatt attttagaga
     2461 atgattgaac agtattgagc aaaactaagt gtggggatgg attgtatggg tttatcagag
     2521 tagggatttg aactgctgtc atatacacca acagtgctca tttatattgc atgctgtctt
     2581 aagggataga tatggaggta tgaaatttgt ctaagaaaag cgttgaggaa attgcccggg
     2641 agatcgcgtt accaataatt gaagaattgg attatgaatt ggtcgatttg gaatataaga
     2701 aagagggtcc ccactggtgc ctaagattat acatagataa gcccggcgga atcactattg
     2761 atgattgcca ggaagctagc aaaagggtaa gcgatgccct tgatttaaag gacccgattc
     2821 cccataatta ctttcttgag gtttcgtccg tcgggttaga ccgtccatta aaaaaggaag
     2881 aggactttgt tagattcaag ggccataaag ttgatataag gctatatagg gcgattgatg
     2941 gatcgaaaag ctttaccggc gatttgatcg gattagtcga aggtgccata cgggtgcaag
     3001 ttggcgaaaa agcgattgat tttgaaaggg atcaagtcgc tattatcaaa ttatcggtgg
     3061 aattttaaag atcggtattt aattgatgat tttagattga aatcagtact ctttgaggag
     3121 gagttaatga aatgaatggg gaatttcttg gtgctctaga ccagatcgcc agcgaaaaag
     3181 ggattgataa ggaaatactg gtagatgcca ttgaagcggc actacaatcc gcctataaga
     3241 aaaactttgg caccagtcaa aatgtgaagg tcaatgtcga taaattaacg ggtgtagtca
     3301 aggtatatgc aatgaaaacc attgtagaag aggttatgga tgaatcccag caaatctttt
     3361 tagaagatgc ccaaagaatt gatccagttt ttagccttgg ggatatcgta gaggtagagg
     3421 tgactcccaa aaactttggt cgcatagcag cccaaaatgc caagcaggtg gtagtgcagc
     3481 gtatacgaga ggccgaaagg gccctaatat atgaaaagtt tattgaaaaa gagaacgaga
     3541 taatgacctg tgtagttcag cgggcagagc gcagaaatgt attcgtcgat atagacgggg
     3601 ccgagggtac tatgcccccc aacgagcaaa tttccaatga gagttatcgg gttaatgacc
     3661 gattgatggt gtatattaca gaagttaaga tgaccaataa agggcctcaa ataacagtat
     3721 ccaggaccca ccccagtttg gtaaagagac tctttgagag ggaggtcccc gaaattatta
     3781 ggggcgaggt tatcataaag gacatagcac gtgaggctgg atccaggaca aaaatagctg
     3841 tttttactca tcaccccagt attgatccca taggggcatg cgtaggtcaa catggtataa
     3901 gggttcagca cattgtggac gaattaagag gcgaaaagat cgatattatt aaatggagtg
     3961 atgatcctag ggagtatatt ggtagcgcct taagccccgc gaggatcgtg aatgtacaaa
     4021 tacatgaggt tgaaaaatcg gcgagggtaa tagttccgga caatcaactt tcccttgcga
     4081 ttggtaagga aggtcaaaac gtgaggttgg ccgctaagct aacgggctgg aagattgata
     4141 taagaagcat atcacaaatg gaaagtattg attctaccca agaagggaat ttggattaag
     4201 gggatgacat gctttgaaaa aaaggaagat cccgctgagg atgtgtgtcg gttgtaatga
     4261 aatacggccc aaaggggaac tcatcagggt ggttcggagc ccccaaggtg atatctatat
     4321 agatcaggta ggtaaaaggc cgggcagagg ggcctatata tgcaagaacc ttgattgctt
     4381 ggaaaatgcc cttaagagca ggaagttgga acgggctttt ggacaaaaaa tcgaccagga
     4441 aatatatcaa aggttgagta tcgaaatggg tgaaggtgag aataattaac gacaaagttt
     4501 atcagttagt tggtctttcg aaaagagccg ggaaattggt ttcaggttcc catgccgtag
     4561 aaaatgcaat caagtctgga aaagcgcaat tagttattgt gagcgaagag gcctcggcta
     4621 atacggttaa aaaattcttt gatatgtgtg aatttagaaa tgtgaatata atcaaaatcg
     4681 gcagcaaaaa ggacttagga gaatgtattg gtaagacaga tcgtaccctt gtagccataa
     4741 cggatgtggc ttttagggac atgatagtag aggtattacc atcgcctata acaaatacgg
     4801 gggtgatcga ttgatgtcaa aaattaatgt gtattcaaca gcaaagaaag taaccgaagg
     4861 cgccagggaa ctaatggata tggtgggaga gacagaaaag ggtattgccg gatataaaaa
     4921 tacaattctg aagcttaggg aaagactaaa tcgggaaact atagagagag aactggaaga
     4981 aaaaaggaag gaacaacggc tggaagaaga atcccgcaga aaaaaagaac agcaaataag
     5041 agcagaagaa agggcaaaga gggaatccgg cccggcggcc ccgggacgaa caggggaaaa
     5101 gaggccaatg gggcccggta cgagggattt gcaaaatcga ttaaaagaaa aatccggtga
     5161 gagtatccgg gtaatagatt caacgggagg aaagcccggg caggacaagg ttgttccgtc
     5221 caagggatat gagaatacgc aaaaacgcag ggagcgtagg agcaaaaaag atttttggca
     5281 agaaggcgaa tcatattcca gaatcaaaaa gttacgggag accaagacta ctagtcagaa
     5341 aagacaagaa aaatccggaa aggtaccaga aaggaaaaaa gccatcataa tgggagaact
     5401 tataacggta aaagatttat cagaaaaaat aggggtttcc gtacccgaaa taatgaaaaa
     5461 gctaatgggt ctaggaatcc tggctacaat aaatcaagac ttggattacg acaccgcagc
     5521 gataatatcg gctgagtttg gaatccaact ggacaaaagg gttattaaaa gcttcgagga
     5581 aatattagag gatgaagatc tggaggatga tcccgattct ttaaaacccc gttcgcctat
     5641 agtaacggtt atggggcatg ttgaccatgg gaaaacatca ttattggatg ctatcagaag
     5701 ttccagggtt acggcccaag aagcgggcgg gattacgcaa cacataggtg cgtataccgt
     5761 aaacgtggat aataggctta taactttttt agatacacca gggcacgagg cctttacctc
     5821 catgagagcg cggggagccc aggttaccga tatcgcaatt ttagtggtag ctgccaacga
     5881 cggcgttatg ccccaaacag tagaagctat caatcatgct aaggaggcgg gggttccgat
     5941 tattgtagcg ataaacaaga tcgatgttac gggtgcgaac cccgaaagag taaagcagga
     6001 gctcactgag catgaactct tggtggagga atgggggggc gataccatag cagttcccgt
     6061 ttctgcactt cgtaatgagg gtattgattc cttgttagag atggtattat tggttgcgga
     6121 aatgcaggaa ctaaaggcca atcccaatcg tcttgcgaaa ggaacaataa tagaggcaga
     6181 actggacaag ggtaggggtc ctgtcgctac ggtcctagtt caaaagggga ctttgagagt
     6241 gggagattcc atggtggcgg gtaccgctta tggaagggtt cgggctatga tggaccatac
     6301 agggaaaagg ttagaaaaag caggtccctc tactcccgtt caagttctag ggctcacgga
     6361 ggttccggaa gccggggata ctatgtattg tgtcgagggc gacaagcttg caaaacaggt
     6421 ctcggaagag cgcaaagaaa aggttagaga actgcagatc agggcctcat cgaaggtatc
     6481 cttagacgag ttgtttaaca agataaaaga gggcgaagta aaggatctaa acctcattat
     6541 caaggctgat gtgcaaggat ctgctgaggc tgtgaagcaa tctttggaaa ggttaaccaa
     6601 tgaggatgta cgaatccgca ctatacacgg gggagttggt gccatcactg aatcagatgt
     6661 catgctggcc accgcctcca atgccattat aatagggttt aacgttcgac cttccccggg
     6721 ggcatccgag ttagcaagca aggaagaaat tgatattcgc ctttacaggg ttatttataa
     6781 cgcaattgaa gatatagagg ctgctatgag gggtatgcta gatcccgaat acaaggaggt
     6841 cattttaggt catgcccagg ttagggagac atttaaggtc cccggtgcag ggattgttgc
     6901 cggctcttat gtcaccgatg gtaagattct aagaaatgca gatgcccgca tagtccgaaa
     6961 cgggatcgta atctacgagg gtaagatcag ttctctcagg agatttaaag acgatgtaag
     7021 ggaggtaacc gccgggtacg aatgcggtat aagcttggta aatttcaatg atatcaaaga
     7081 agatgatgta attgaggttt ccattcaaga agaaattaag aggtaatatc aatacaaagg
     7141 gcaggtgggt atcatgaaac accaaaggat cgataggatt tctgaagaga taaaaaagga
     7201 aataagcaga attatccggg aggatgtaaa ggacccacgt ataggacaga tggcttctat
     7261 tcttcgcgtg gatgtatcag gggacctgcg ccatgctaag gtatatgtga gcgttttagg
     7321 cgatgaaagg gaaagggcat ccaccatagc aggtctatcc agagcggccg gtttcataag
     7381 aaagggattg ggccgggttc ttactattag atatatacca gaactaaatt ttatcctgga
     7441 tacttctatt gaatatagcg tggatatcag caagaaaata atggagatta aaaaacaaca
     7501 ggagcagtcc aatggggatg attgatatca tccaaccaat agacaggagc gattccattg
     7561 cacttattac ccatatgaat cctgatgggg atgcggtggg aagcctgatt gccctaatgc
     7621 gggccttgga taaaatgggt aagtcggtag atgcttattg tcaggatgag gtgccggggg
     7681 ccctcaattt cttagacggc gtcgccagga taacaaggcc cattgatcca ataaaacgat
     7741 atgatttagc aatcgctttg gattgcagtg accgggggag gatgggggac tgcgcaacta
     7801 ttatggacat ggcaactagt tcggctaata tagatcacca tataagcaat accttttatg
     7861 ctgatataaa catcgtggac gggggtgcct ctgcaacggg ggagatgata tacgagttgg
     7921 tagaattgct aatagattca gctgataaag gtatagccga ggccctctat accgctatag
     7981 tgtcagatac gggcgggttt gcttttagta atactaccgc aaaaacccat agaacggctg
     8041 ccaatttaat tgaatggggt gccgagacag caaag
//
LOCUS       DASRNX010000219        13872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_468248,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000219 DASRNX010000000
VERSION     DASRNX010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 13872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13872
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468248"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..580)
                     /gene="ssrA"
                     /locus_tag="VFD89_08585"
     tmRNA           complement(226..580)
                     /gene="ssrA"
                     /locus_tag="VFD89_08585"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(664..1131)
                     /gene="smpB"
                     /locus_tag="VFD89_08590"
     CDS             complement(664..1131)
                     /gene="smpB"
                     /locus_tag="VFD89_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZJ58271.1"
                     /translation="MATDSIKVIAQNRKARHDYFVEETFEAGIVLAGTEVKSIRLGKV
                     NLRDSYADVRNGEVFVNNMHISPYEMGNITNKDPIRSRKLLLHKREINRLLGYVQLKG
                     LTLIPLRIYLKGGLVKLELAVARGKRQFDKRQDIARRDAQRDMDRAFRESQKL"
     gene            complement(1285..2088)
                     /locus_tag="VFD89_08595"
     CDS             complement(1285..2088)
                     /locus_tag="VFD89_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZJ58272.1"
                     /translation="MRDIKGFNKQDMGNGVWRISEGNPEAGISNFVDAYLVCGQTRAV
                     LVDTLMGEGNLYDIIREITALPVDVIMTHGHGDHAGRELKNLADKGLSLFMKPVDYEV
                     YTNMSFEPVSRESFQDVSHGQVFDLGGRHLEIIEVPGHTPGSIVVLDRGNQLMFSGDS
                     IGSGTIWMQLAHSLPLEQFLDVLRRLEREVEDLNDLVIYPGHFYQMRERLRKQYISEM
                     RELTENLVSGKTKGVPIEDEETTVRFPNALQASLGAMYSYCYDPDNIFK"
     gene            complement(2341..4473)
                     /gene="rnr"
                     /locus_tag="VFD89_08600"
     CDS             complement(2341..4473)
                     /gene="rnr"
                     /locus_tag="VFD89_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HZJ58273.1"
                     /translation="MILDIKEKIVGLLREKNTPPLFLDKLMEALEFSQSDRGIFMGIL
                     DDMVEGGQLVRTRKKKYALPETLGFLVGRLQGNARGFAFFIPDSEEEKDVFIPPENLN
                     GAMHKDRVMIRVFGDKYSTASSREGEVYKVLWRANTSIVGTFERDKNFGFVVPDDSRI
                     SDDIFVSKDSMLDARTGYKVVVEVFRWPDSRRNPEGKIVEILGHKDEPGTDILSIIRQ
                     FNLPEDFSEEILSAARKIPQKVSNEDIQRRRDLRDLKTFTMDGADAKDLDDAISIERN
                     KKGNFILGVHIADVNHYIFENSPLDKEALTRGTSVYLVDRVIPMLPRELSNGICSLNP
                     NEDRLAMSVFMEVEQGGKVVDYSIEETVIRSQKRMVYEDVTMILEDGEKDQAVMVDYH
                     DFLDELRDAEELCKILNEERTKRGSIDFNLDETQIILDEDGKPVDIRPAVRGISNRII
                     EEFMLLCNETIAQHIFWQELPFMYRVHEDPDIEKMLDFNEFIHNFGYNLKGIGEGIHP
                     KVLQRLLADIKDKPEERIISTIMLRSLQKARYSHENLGHFGLASKNYCHFTAPIRRYP
                     DLMIHRIIKDSIHGKLTKKKAKQMEEMLPDMAKHCSSRERTADEVERETGDLKKVEFM
                     ADKIGEEFDGIISGVTGYGLYVQLPNTIEGLVRISSIEDDYYNFVEKHYCLIGERHRR
                     IFRIGDPARIKVLNVNMVMRNIDFVLAD"
     gene            complement(4857..5093)
                     /gene="secG"
                     /locus_tag="VFD89_08605"
     CDS             complement(4857..5093)
                     /gene="secG"
                     /locus_tag="VFD89_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZJ58274.1"
                     /translation="MGALKLAATIVLVLFELFLIIVVLLQTGKSAGLSASFGGGSETF
                     FGKNKARSFEGRLQRLTTISAIGFILVSIILAIM"
     gene            complement(5216..6508)
                     /gene="eno"
                     /locus_tag="VFD89_08610"
     CDS             complement(5216..6508)
                     /gene="eno"
                     /locus_tag="VFD89_08610"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZJ58275.1"
                     /translation="MIIIDVLAREIVDSRGNPTVEVEVALESGAVGRAAVPSGASTGA
                     FEAVELRDDDKARYLGKGVLKAVDNVNDIIAPEVLGMSALDQIGVDQVMIDLDGTHNK
                     GKLGANAILGVSLAVAKAAANGLGLPLYQYIGGVNAKVLPVPMMNIINGGEHADNTVD
                     IQEFMVMPVGAKSFREALRMSAEIFHNLKTVLGAKGYSTAVGDEGGFAPDLKTNEEAI
                     EVILEAVEKAGYKPGDDIRIAIDAAATELYNEEDGKYHFPGEGVVRTVEELVDYYSGL
                     ADKYPIISLEDGIAEEDWEGWALLTDKLGDKIQLVGDDLFVTNTERLSRGIDLKVANS
                     ILIKVNQIGTLTETLDSIEMANRAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGA
                     PSRSDRVAKYNQLLRIEEELGELAQYPGLDAWFNLENK"
     gene            complement(6577..8118)
                     /gene="gpmI"
                     /locus_tag="VFD89_08615"
     CDS             complement(6577..8118)
                     /gene="gpmI"
                     /locus_tag="VFD89_08615"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZJ58276.1"
                     /translation="MAKNLTALVIMDGFGLSDSKEANAIYEASTPNLDRYRNEYPSGQ
                     IQASGLDVGLPDGQMGNSEVGHLNLGAGRIVYQDYTRVSKAIEDGIFFDNPAFLSAIN
                     NAKDKGTKLHLMGLLSDGGVHSHIEHLYALVELAKQKGLDRVYIHCFMDGRDVPPASG
                     KGYILVLEEKLKQLGVGKIATVMGRYYAMDRDNRWERVKVAYDAMVLGQGITAGSGAE
                     AMQKSYDQKETDEFVRPTVILEDGHPVATIDEDDSIIFFNFRPDRAREITRSLIDAEF
                     DGFVRDKGYFPVSYVSMTQYDETFKGIGVAYSPEELTSTFGEYISGHEKTQLRIAETE
                     KYAHVTFFFNGGVEVPNKGEDRALIPSPKVPTYDQKPSMSAFEVTEEAERRIASGKYD
                     CIILNFANCDMVGHTGVMEAAVEAVETVDTCVGRVVEAIRGVGGRVIVTADHGNAEKM
                     LDHDTGEPHTAHTNNPVPLIFIDDNRRNVTVREGGRLSDIAPTLLELMGLPKPPDMTG
                     ESLLR"
     gene            complement(8331..9077)
                     /gene="tpiA"
                     /locus_tag="VFD89_08620"
     CDS             complement(8331..9077)
                     /gene="tpiA"
                     /locus_tag="VFD89_08620"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003371300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZJ58277.1"
                     /translation="MRKPIIAGNWKMNKTPTETTDLIDGLKPLVKDAQADVVVCPSFV
                     CLAAAAEALKGSNIALGAQNMYFEENGAFTGEVAPNMLKDLGVQYVIIGHSERRQYFG
                     ETDETVNKKVTVAFAHDLIPIMCVGETLEQREESITEKVVERQTREGLKGLSSKEAER
                     VIIAYEPVWAIGTGKTATSQQANEVISFIRGIVVDMFGKEVAEKIRIQYGGSMNAKNA
                     SELMAMPDIDGGLIGGASLKAQDFAKVINY"
     gene            complement(9153..10337)
                     /locus_tag="VFD89_08625"
     CDS             complement(9153..10337)
                     /locus_tag="VFD89_08625"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZJ58278.1"
                     /translation="MNKKTIEDINVANKRVLVRVDFNVPLDNEGNVTDDTRIRAALPT
                     IKYLLDNRAKVILMSHLGRPKGEFKSEFSLRPAAKRLSELLAMDVNMADDVIGDSAKK
                     AVEGIGEGQVVLLENLRFHKEETKNDPEFAKRLAAFGEVYVNDAFGTAHRAHASTEGV
                     AKYLPAAAGYLIKKELEVMGKALSNPENPFIAILGGAKVSDKIGVIENLIEKVDTLII
                     GGGMAYTFFKARGYEIGDSLLEADKVELAKSLEEKAAAKGVKFLLPVDNVVAREFSAD
                     SEHKVVNSDAISQGWQGLDIGPESRQIFGEEIKNAKTVVWNGPMGVFEMPAFAEGTKA
                     IAEYMSECQGTTIIGGGDSAAAVEQMGYADRMTHISTGGGASLEFLEGKTLPGVAALD
                     DK"
     gene            complement(10452..11465)
                     /gene="gap"
                     /locus_tag="VFD89_08630"
     CDS             complement(10452..11465)
                     /gene="gap"
                     /locus_tag="VFD89_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZJ58279.1"
                     /translation="MAVKVAINGFGRIGRNAFKIALEKYADELEFVAINDLTDPATLA
                     HLLQFDSCFGKFDGDVEVKEDALVVNGKEVKIFAERDPANLPWKELGVEVVMECTGIF
                     RSKETAGKHIEAGAKKVIISAPAKNEDITLVMGVNEDKYDAENHHIISNASCTTNCLA
                     PFAKVLNDNFGIKKGLMTTVHSYTNDQNILDLPHSDLRRARAAAESIIPTTTGAAAAV
                     ALVLPELKGKLNGFAMRVPTPTVSVVDLVVELAKPATAEEINAAMKEASEGPMKGILG
                     YEERPLVSIDYKKDDRSSIVDALSTMVIDGNMAKVVSWYDNEWGYSARIVDLVNYIAE
                     KGI"
     gene            complement(11579..12610)
                     /locus_tag="VFD89_08635"
     CDS             complement(11579..12610)
                     /locus_tag="VFD89_08635"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HZJ58280.1"
                     /translation="MLQSQALLKKIVPEFIELFDRRYTILRSIYSMQPVGRRTLASAL
                     KMGERTVRNETSFLKDAGMLTTHPSGMEITREGEIVLDGLREFTHNIRGLYNLERQLE
                     EILNLRRVLIIPGDADEDELVLSDIGRTAAGILREVIKNGDVVSITGGNTVGKVADAL
                     PYIGELKDVMVLPARGGMGGNVEYQSNTLAAVFAKKLGGHYKMLHVPGQLRAEAAKTL
                     INEPDVKSVIEYLKNTNILIYGMGKAEDMAARRNLTSDQMGIIRREKAVAEAFGYFFN
                     MKGDIVFSSNPIGISIRDLKHIPTVIGVVGGKQKAKAVMAVIGNCPDSILVMDEGIAM
                     GIMDTHNSK"
     gene            complement(12741..13199)
                     /locus_tag="VFD89_08640"
     CDS             complement(12741..13199)
                     /locus_tag="VFD89_08640"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HZJ58281.1"
                     /translation="MPLNIVLVEPEIPQNTGNISRTCVVTGATLHLVKPLGFSVDDHH
                     LKRAGLDYWSLLDIRYYDSLDDFFEKNPRGDFYFSTTKAKRFYTDEKYARDSFLFFGK
                     ETAGLPQWLLKKYQDRCIRIPMREGVRSLNLSNSVAIIVYEALRQLNFPF"
     gene            complement(13347..13796)
                     /locus_tag="VFD89_08645"
     CDS             complement(13347..13796)
                     /locus_tag="VFD89_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZJ58282.1"
                     /translation="MKDMVPFRRRNGLTPIDLFKGFFGRDLMDDFFGNDMLSGGMTGG
                     FRADIKETEKEYVVEAELPGYNKEDIDIDLVNDRLTISAKKNEEIKEEKENYIRRERR
                     MGQASRCFLVSGIKNDEVKAEYADGLLKVTLPKEEEGRTRNTRIDIN"
BASE COUNT         3415 a         3667 c         2673 g         4117 t
ORIGIN      
        1 gtttattagt gtaatgttgt cgaaggtcga accatatctt gtaatccaac agaaaaaaca
       61 aagggtgcgg ctgatcctca ccaaatataa atcacttacc cccggaaatg gcaggtatcc
      121 cctagaacta ttaaaggcta aagatgactt ttaccagtgt tttatttcac tctagatagg
      181 taaaaggctt accaattgtc ttaacaatta gtaagcctta ttatttggtg gaggcgaggg
      241 gaatcgaacc cctgtccgaa gatatacaga taccagcatc tccgagcgca gtcagtgttt
      301 tatcattccc tccaccggac tcccactaac aggattccgg ctttagtagc ttcataaatt
      361 ccggcctaac gcaaagctta gttaggttcg ttccccgcta atttgtcgcc ttttcctata
      421 gtgcgggtac tatagggaag acgtagctgc tttaagcagc agctaaagcg taattatcgt
      481 tgtttgcttt taatttaagt tccaggttgt tgatgcggcc ccagaatccg cagctcgctt
      541 ctcatacctc tattatcccc gtcgaaacca atacgccccc atattaatag atcataagca
      601 aacgcaatat gggatactgg caaaatcaga ttggatacat tgtgaaagca cttatctttc
      661 taactaaagc ttttggctct ccctaaaagc tctgtccata tccctttggg catcccgcct
      721 ggctatgtct tggcgcttgt caaactgcct tttgcctcgg gcaacagcca gttccagctt
      781 tacaagtccc cctttgagat atatcctaag gggaatcagg gtcaacccct ttagctgaac
      841 ataacctaaa agcctattta tctccctttt gtggagcaat agcttgcggc tccgtattgg
      901 gtccttattt gttatattcc ccatttcgta agggcttata tgcatgttgt tgacgaagac
      961 ttcgccattt cttacatcgg cataactatc cctgagatta acctttccaa ggcgtattga
     1021 cttgacctcg gtgcctgcca gcactatgcc ggcctcaaag gtttcctcaa cgaaataatc
     1081 atgcctagct ttacgatttt gggcaataac cttgatacta tccgtagcca atttctacac
     1141 ccaccccgat aaaataaccc tatctgctgt gcggggcaaa tagggaatct ccagtcgcat
     1201 tattatatta acatacccgc gaaactctgt caagtacgcc aaaatccaga tttatcgttc
     1261 tgggcggatc atatgcttca tattctactt gaaaatatta tccggatcat agcaatagct
     1321 gtacatcgct ccaagacttg cctgaagggc attaggaaat ctcacggtgg tttcttcatc
     1381 ttcaatgggg accccttttg ttttccccga gacgaggttc tctgtgagct ctctcatctc
     1441 acttatatat tgttttctca gtctttccct catctgatag aagtgccccg ggtatattac
     1501 aagatcattt agatcttcga cctccctttc cagacgcctg agaacatcaa ggaattgctc
     1561 taggggcagt gaatgggcaa gctgcatcca tatggttccc gagcctatgg agtcgccgga
     1621 aaacataagt tggttccccc tgtccaaaac cacgatgctg ccgggagtat gtcccggaac
     1681 ctcgataatt tccaaatggc gaccgcctaa atcaaaaact tgaccatggg acacatcttg
     1741 aaagctttcc ctactgacgg gttcaaagga catattggta tagacctcgt aatccaccgg
     1801 tttcataaaa agactgagtc ctttatctgc taggttttta agctcccttc cggcatgatc
     1861 cccgtggcca tgggtcatta ttacatccac gggaagggca gtaatttccc tgatgatatc
     1921 ataaaggttt ccttcgccca taagggtgtc tacaagtacg gcccttgttt ggccgcatac
     1981 cagatatgca tcaacaaagt tggaaattcc ggcctcggga ttcccttcac tgatcctcca
     2041 aacaccgtta cccatatcct gcttattaaa gccttttata tccctcataa cagtaacaac
     2101 actcccttca atatagtatg ctaatgtgtt atggccctca atagccatta ccgatatatt
     2161 taacgggtgt aactatatgt tttacgacaa acattctaca ttagtatatg ggtgtcgtaa
     2221 aacattatcc tcaattcata tgttacgaaa aatacattgc tttaacgcga aatcagataa
     2281 cttaactgat tttgtattga aagatctttt tcataacata tatagccata ttataccaca
     2341 tcaatcagcc aatacaaaat ctatatttct cataaccata ttcacattca atacctttat
     2401 tctagccggg tcccctatcc taaagattcg tctgtgcctt tcgcctatga gacaatagtg
     2461 cttttcaaca aagttatagt aatcgtcctc tatggagctt attcgaacca agccctctat
     2521 ggtattggga agctggacat ataggccata tcccgtcacc ccggaaataa tcccgtcgaa
     2581 ctcttcgcct atcttatccg ccatgaattc tactttttta aggtcaccgg tttctcgctc
     2641 cacttcgtcg gcagttcgct cccgacttga acaatgcttt gccatatcgg gcagcatctc
     2701 ctccatctgc ttcgccttct ttttcgttag ctttccatga atggaatctt tgattatgcg
     2761 atggatcatt aggtccggat agcggcgaat gggtgcagta aagtggcaat agttcttgga
     2821 agccaaacca aaatgtccga ggttttcatg actgtatcga gccttttgga gggatctcag
     2881 cattattgtg cttattatcc tttcctcagg cttgtcctta atatcagcta aaagtctctg
     2941 aagcaccttt ggatggatac cctcgcctat cccctttaga ttgtatccaa agttatggat
     3001 aaactcgttg aaatccagca tcttctctat atccggatcc tcatgaaccc tatacataaa
     3061 ggggagctcc tgccaaaata tgtgctgggc aatggtctca ttacagagca gcataaactc
     3121 ctcaatgatc ctgttggata tacccctaac cgccggcctt atatcaacgg gctttccgtc
     3181 ttcgtccagt atgatctggg tctcatccag gttaaaatcg atgcttcccc gttttgttct
     3241 ctcctcattt aggattttgc ataactcttc ggcatcccgc agctcatcta agaaatcgtg
     3301 gtaatctacc ataaccgcct gatccttttc cccgtcttcc aggatcatgg ttacatcctc
     3361 atagaccatc cttttttggc ttcttatgac ggtctcctct atggaatagt ccacgacctt
     3421 cccgccttgt tccacctcca taaagaccga catggccaag cggtcctcat tggggtttag
     3481 gctgcagatc ccgttagaga gttccctagg gagcatcggt atgactctat ccaccagata
     3541 aacgctggtc cccctggtca gcgcctcctt gtccaatggg ctattttcaa agatgtaatg
     3601 gttgacatcc gctatatgta ccccaagtat aaagttgcct tttttatttc tttctatgga
     3661 tatggcatcg tctaaatcct tggcatcggc cccgtccatg gtaaaggttt taagatcgcg
     3721 cagatccctt ctcctttgga tatcctcatt agacaccttt tggggtatct ttctggcggc
     3781 gctgagaatt tcttccgaaa agtcctccgg aagattgaac tgacgaataa tagacaatat
     3841 gtccgtcccg ggttcatcct tatgtcctag gatctccact attttcccct cgggatttct
     3901 cctagaatct ggccacctaa agacctccac cactaccttg tatcccgttc tggcatctaa
     3961 catactatcc ttggaaacga agatgtcatc gcttatcctt gaatcatcgg gcaccacaaa
     4021 accaaagttt ttatcccttt cgaaggttcc cactatggac gtgtttgctc gccagagcac
     4081 cttgtagact tcgccttccc tagaggatgc ggtgctgtat ttgtccccga atacgcgaat
     4141 cataaccctg tccttatgca ttgccccgtt taaattctcc ggcgggatga atacgtcctt
     4201 ttcttcctcg gaatcgggaa tgaaaaatgc aaaccccctg gcgttgcctt ggagccgtcc
     4261 taccaaaaac cctaaggttt cgggcagggc atactttttc tttctagtcc ttaccagctg
     4321 accgccctct accatatcgt ctaatattcc cataaagatt ccccggtcgc tctgggaaaa
     4381 ctccaaagcc tccatcaatt tatcaagaaa taggggaggc gtatttttct cccttaaaag
     4441 tccaactatt ttttctttaa tatccaatat catacctcct ggatgtttta cgataatcat
     4501 tttaaaatat acttcgaata ctgcagagca ttttccttat gattctttag tttttccact
     4561 ttctatgtat tataatcagt cccggggcca tatatcctcc ccttcataaa aaggataacc
     4621 catggagctg ggcctaaagg gttcatccgt ctgccccata ataccttcag ttcttaccat
     4681 aaatataaaa aacgaccgcc gggggtcgtt cttgaaaacc tttaaacatc gtaaccaact
     4741 gcatataatt atagcacacc actaagacgg aatgggggat tctttatcaa tgcctatccc
     4801 gtcccggcaa aatatgccga ttttaacagg caaagaatac tttcccgtcg tccccgctac
     4861 attatggcta aaatgatgga aacaaggata aagccaatgg ctgaaatggt agtaagcctt
     4921 tggagtctgc cttcaaagct tcgggcctta ttcttcccga aaaacgtttc agatcctcct
     4981 ccaaaagatg ccgacagccc ggccgacttt cccgtttgaa gcagcactac gatgatcaaa
     5041 aatagttcaa ataacaccag cacgatggta gcagctaact taagcgcgcc catactatat
     5101 ccctcccttt gcggataaag ctctaacaaa aatattgtac catagggtgt tgttaaaatc
     5161 aagaaaagaa aaacgaaagg gggtggattc tctcaccccc taatttcttc tgctcttatt
     5221 tgttctctaa gttaaaccac gcatcgagac cgggatattg ggccagctca cccaactcct
     5281 cttcgatcct caatagctga ttatatttag cgaccctatc ggatctggaa ggtgcaccgg
     5341 tcttaatctg tccggcatta accgctacta ccagatcggc aatggtcgta tcctcggttt
     5401 caccagaacg atgggatacc acggctgtgt acccggcgcg gtttgccatt tctatagaat
     5461 ctaaagtctc ggttagggta ccgatctgat tcactttgat taagatagaa ttggcaacct
     5521 ttaaatcgat gcccctagat agacgctcgg tattggttac gaagagatcg tcccctacca
     5581 actggatctt gtctcccaat ttatccgtca gcagcgccca gccttcccaa tcttcctcgg
     5641 ctataccgtc ttctaaggat ataatcggat acttatcggc aagccctgaa tagtagtcga
     5701 ccagttcctc aacggttctg actacacctt cacccgggaa gtgatattta ccgtcttcct
     5761 cgttatagag ttccgttgct gcagcatcga tggcgattct gatatcgtct ccaggcttgt
     5821 atccggcctt ttctacggcc tcaaggataa cctctatggc ttcctcgttg gtcttaagat
     5881 ccggagcaaa tccaccctcg tcacctactg cggtgctgta accctttgcg ccaagcacag
     5941 tctttaggtt atggaaaatc tctgcagaca ttctgagagc ttccctaaag cttttggccc
     6001 ctacaggcat gaccataaat tcttggatgt ccacggtgtt atccgcatgt tccccgccgt
     6061 ttattatgtt catcataggc accgggagaa ccttggcatt aaccccgccg atatactggt
     6121 ataggggtaa tcccaatccg tttgcggcag cttttgccac tgccagggat acccctaaaa
     6181 tggcgttggc tcccaatttt cccttgttat gggttccgtc taagtcgatc ataacctgat
     6241 caacgcctat ctggtccagg gcactcattc ctaaaacctc aggtgcaatt atatcgttta
     6301 cattatctac cgcttttaag acccctttgc ctaagtatct ggccttatcg tcgtccctta
     6361 attcaacggc ttcaaaggcc cccgtagagg caccggacgg gactgcggcc ctgccgacgg
     6421 ctccgctctc tagggctacc tctacctcta cggtagggtt acccctggag tcaacaatct
     6481 ctctggctag tacatcaata atgatcataa aatttatctc ctttcgtttg tctatagatt
     6541 aacaatatcg taactaaatt tatcctccgt ttaaaactac cttaagagac tctcccctgt
     6601 catatcgggg ggtttgggta gccccatgag ctctaaaagg gtgggagcga tatcggagag
     6661 ccgcccgcct tctcttacgg taacattcct gcgattatcg tctatgaaga ttaacggtac
     6721 cgggttattt gtatgggccg tatggggttc tccggtatca tgatccagca tcttttcagc
     6781 gttaccatga tccgcagtca caattactct gccccctacg cctcttatag cctcgactac
     6841 cctgccaacg catgtatcta cagtctccac cgcctcgacg gcagcctcca ttacgccggt
     6901 atgacccacc atatcgcagt tggcaaagtt taggatgata caatcgtatt tgccggatgc
     6961 tatacgcctt tccgcctcct ccgttacttc aaaggcgctc atggagggct tttggtcgta
     7021 ggtaggtacc ttgggagatg gtataagggc tcggtcctcc cctttgttgg gcacttcaac
     7081 accgccgtta aagaagaagg tcacatgggc atacttttcc gtttcagcta ttcgaagctg
     7141 ggttttctcg tgcccgctta tatactcccc aaaggtactg gtaagctcct caggagaata
     7201 agctacccct atacccttaa aggtctcatc atattgggtc atagatacat agcttacggg
     7261 gaaatagcct ttatccctga caaagccatc aaattccgca tcgatgaggc tcctggtaat
     7321 ttccctggcc ctatcgggtc taaagttaaa gaagattata gaatcatctt cgtctatggt
     7381 cgctacggga tgtccatcct ctaatataac ggtgggcctg acgaattcgt cggtctcttt
     7441 ctggtcatag gatttttgca tcgcctcggc gccgctcccg gccgttattc cctggcccag
     7501 caccatagcg tcgtaggcca ctttgaccct ttcccagcga ttatccctgt ccatggcata
     7561 atatctgccc atgacggtgg caattttccc cacccctaat tgttttagtt tttcttcgag
     7621 gaccaagata taaccctttc cgctggccgg cggaacatcc ctgccgtcca taaagcagtg
     7681 aatatatacc ctatcaagtc ccttttgctt ggccagctcc actagggcat ataagtgctc
     7741 tatatggctg tgaaccccgc cgtcggataa aagccccatg agatgaagct tggtgccctt
     7801 gtcctttgcg ttgtttattg ctgacagaaa tgcgggatta tcaaagaata tcccgtcctc
     7861 gatagccttg ctgacacggg tataatcctg ataaactatc ctgccggccc ctaggttaag
     7921 atgccccacc tcggaatttc ccatctgccc gtcaggaagc ccgacgtcca atccgctggc
     7981 ttgtatctgc ccgctggggt attcgtttct atatctatcc aagttaggcg tagacgcctc
     8041 gtatattgcg tttgcttcct ttgaatcgct gagtccaaac ccatccatta tcaccagggc
     8101 agttaaattc ttggccataa gtccctccta atatgcaagt tccaaaaacg catttgcttc
     8161 gtagcctggg tataatacca ataatccaag tcattaaaac ttcaacttgc cgattattta
     8221 ctaacgattt ttaataatct attacggtcc agcctttttg acaatcttta ataatttaat
     8281 ctattacgct agttaaaaat gccggcaata acttttgaac gcccatttta ttaatagtta
     8341 attacctttg cgaaatcctg ggctttcagg ctggcaccgc cgatgagtcc gccgtcgatg
     8401 tccggcatag ccataagctc cgatgcgttt ttggcattca tgctaccacc atattgtatc
     8461 cggatctttt ctgctacttc ttttccaaac atatctacga caatgcctcg aataaaggaa
     8521 atcacttcgt tggcctgctg gctggtagcg gtctttccgg ttccaatggc ccatacgggt
     8581 tcgtaggcaa tgatgaccct ttcggcctct ttactgctaa gtcccttaag accttcccta
     8641 gtctgccttt cgactacctt ttcggttata ctctcttccc tctgctccag ggtttcccct
     8701 acacacataa tggggatcag atcatgggca aaggctactg tgaccttttt attcaccgtc
     8761 tcatcggttt ccccaaaata ctgtcttctc tcagaatgcc ctataataac gtattgtacc
     8821 cctaggtcct tcagcatatt tggggccact tccccagtga aggcaccatt ttcttcaaag
     8881 tacatgttct gcgctcccag ggctatattt gaacccttta gggcctcagc cgcagcagcc
     8941 agacatacaa aagaagggca gaccacaaca tctgcttggg catcctttac aaggggtttt
     9001 aaaccatcta tcaaatccgt tgtttccgtc ggtgtcttgt tcatcttcca gtttcctgca
     9061 ataatcggtt ttctcatctt catttctcct aacatcctaa ctataccgcc cgaaaagcgg
     9121 gcggtatagt ctacatagtc cctatgtata tactatttgt catccagggc agctaccccc
     9181 ggtaaagtct ttccttcaag aaattctagt gaagccccac cgccggtgga aatatgggtc
     9241 atcctgtcgg catatcccat ctgctctact gccgcagcag aatcgccacc gcctataatg
     9301 gtggtaccct gacactcact catatactcg gctatggcct tggtgccttc agcgaaggcg
     9361 ggcatttcaa atacgcccat gggtccgttc cataccacgg tcttcgcatt ttttatctcc
     9421 tcgccgaaga tctggcgaga ttcgggccct atatccagcc cctgccaacc ctgggaaatg
     9481 gcatcgctat ttacaacttt atgctccgaa tcggcgctga actcacgggc taccacattg
     9541 tcaacgggca ataagaactt aacgccctta gccgctgcct tttcctccag ggatttggcc
     9601 agctccactt tatcagcttc aagcaaggaa tccccaatct catagcctct agccttaaag
     9661 aaggtatagg ccatccctcc ccctatgata agggtgtcca ccttttcgat taggttctca
     9721 attacaccta tcttatcgga taccttggct ccgcctaaga tggcaataaa gggattttcg
     9781 ggattgctca gagccttgcc catgacctca agttccttct taataagata cccggctgca
     9841 gcaggaagat atttggctac cccttcggta gaggcatgag ccctatgggc cgtaccaaaa
     9901 gcatcattta catagacctc accgaaagct gccaatcttt tggcgaattc cggatcgttt
     9961 ttggtctcct ccttatgaaa gcgtaggttt tccagtagca caacctggcc ctcacctatt
    10021 ccctctactg cctttttggc gctgtcgcct attacatcgt cagccatatt tacatccatg
    10081 gcaagaagct ccgataacct tttggccgca ggccttaggc taaactcgga cttaaattct
    10141 cctttgggcc tgccaaggtg agacattaaa atcaccttgg ccctattatc aagaagatac
    10201 tttatggtag gaagggccgc tcttatcctc gtatcatcgg ttacattacc ttcattatca
    10261 aggggtacat taaaatctac gcgaaccaaa actctcttgt tcgcaacgtt gatatcctca
    10321 atagtttttt tattcacgtg atccgctcct ttcaggccta aaattcaaag gcaggatacc
    10381 gaataaccgg gggcaagatt aagatgcccc cagtttcggc cccctgtctt ctaacttcta
    10441 acctctagaa attatatacc cttctcggct atatagttca caaggtcaac aattctggcg
    10501 gaatatcccc actcgttatc gtaccaagat acaacctttg ccatgttgcc gtctataacc
    10561 atggtggaaa gtgcatccac gatggaggat ctgtcatcct tcttatagtc tatggatacc
    10621 aaaggcctct cctcatatcc taagatccct ttcatcgggc cttcggaagc ttccttcata
    10681 gctgcgttta tctcttcagc ggtagcgggc tttgcaagct ctactaccag gtcaacaacc
    10741 gatactgtag gtgtaggtac cctcattgca aatccattta atttgccttt gagctcagga
    10801 agtaccagcg caactgcggc tgcggctcct gtggtggtag gtataatgga ctctgctgct
    10861 gcccttgccc ttctcagatc gctatggggc aggtctaaga tattttgatc gttggtgtat
    10921 gaatgaacgg tggtcataag accctttttg attccgaaat tgtcgtttaa tacctttgca
    10981 aagggtgcaa gacagttggt ggtacaggat gcgttggaga tgatatgatg attttcagca
    11041 tcatatttgt cctcgtttac gcccattacc aatgtaatgt cttcgttctt ggcgggagca
    11101 gagatgatta ccttcttagc gcctgcctcg atgtgcttac ctgcggtctc cttggatctg
    11161 aagatacccg tacattccat tacaacttca acgccaagct ctttccatgg caggttagcg
    11221 ggatccctct cggcaaatat cttgacttct ttgccgttta ctaccaaggc atcttccttt
    11281 acctctacat caccatcgaa cttaccaaag caagagtcga actgaagtag atgtgctagg
    11341 gtcgcgggat cagttaaatc atttatagct acaaattcca attcatcggc atacttttcc
    11401 aatgctattt taaaagcatt ccttccgatt cttccaaaac cattaattgc aacttttaca
    11461 gccattctga aattcctcct taatattttg ctgttaacca gcattataat aattatataa
    11521 acaaaggact agacttgaat cataaataat ccgtctatgg tctttgtcta gaacaacctc
    11581 acttagaatt atgggtatcc ataataccca ttgcaatacc ctcgtccata accagtatgc
    11641 tgtcgggaca atttcctatg acggccataa ccgctttggc cttttgctta cctcccacta
    11701 cgcctatgac ggtggggata tgcttaagat cccgaatact gatccctata gggttcgacg
    11761 agaatactat atcccccttc atattaaaga aatatccaaa cgcttccgct acagcctttt
    11821 cccttcttat tatacccatt tggtccgaag ttagatttct tctggccgcc atgtcttcgg
    11881 cttttcccat accatatatt aaaatattgg tgttcttcag atattcgata acagacttta
    11941 catcgggctc gtttatcaaa gtcttagcag cttcggccct caattggccc gggacatgaa
    12001 gcatcttgta gtgcccgccc agcttttttg caaagacagc cgccaaggta ttggattggt
    12061 attcaacatt accgcccata ccgccccttg cgggaagcac cataacatcc tttagttcgc
    12121 ctatgtacgg aagggcatct gctacctttc ctacggtatt gccccctgtt attgagacca
    12181 catcgccgtt ttttatgact tctcttaaga tgccagctgc cgttctgcca atgtcggata
    12241 atactaactc atcttcatcg gcatctccag ggattattag gaccctcctt aagtttagga
    12301 tctcctccag ctgcctttca aggttatata gaccccttat gttatgggta aattccctaa
    12361 gaccgtctag gactatttcg ccctctctgg ttatctccat gccggaagga tgggtggtca
    12421 gcatccctgc atcctttaaa aagcttgtct cattacggac ggttctctcc cccatcttca
    12481 aggcggaggc aagggttctc cggcccacag gttgcataga atagatgctt cgaagtatag
    12541 tatagcgcct atcaaataat tctatgaatt ccggcactat ctttttcagt agtgcttggg
    12601 actgtaacat acttcactcc attctggaca tttcttgtcc caccgtgcat aaattgaccc
    12661 gccgtttccc ataaaaaggg tgctccatca cccgtatcta ttttacatta aatccccctg
    12721 tatttcaaga gaaaaattaa tcaaaatgga aaattcagct gtcttaaggc ctcatataca
    12781 ataattgcaa cggagttgga taggttaagg gatctgaccc cttccctcat ggggatgcgt
    12841 atgcagcggt cctgatactt ttttagtagc cattggggaa gccccgccgt ctccttaccg
    12901 aaaaaaagaa aactgtcacg ggcgtatttt tcatcggtat agaacctctt ggccttggtg
    12961 gtagaaaaat aaaagtcccc ccttggattc ttttcaaaaa agtcatccag ggagtcataa
    13021 taccttatgt ccaataggct ccaataatcc aaacccgctc tttttaggtg atgatcatct
    13081 acagaaaagc ccaaaggctt tacaagatgc aatgttgcgc ctgtaacgac acaggtgcga
    13141 gaaatatttc ccgtgttttg cggaatctcc ggttcaacta aaacaatatt gagtggcata
    13201 actccccgtc cttccgtctg catatcaatt tttccatgaa tgattttata acagaatcat
    13261 tttaccaaat ttcatgacgg aaatacagta tttttttatc cgtaaacaaa aaaacagggc
    13321 ccgatcggcc ccgctatgga aatcatttaa ttaatatcta ttctagtatt tctggtcctg
    13381 ccctcttcct ctttcggcag ggtcaccttt aagagcccgt ctgcgtactc cgccttcacc
    13441 tcgtcatttt tgatgccgga taccaagaag cacctagatg cctggcccat gcgcctttcc
    13501 cttctgatgt aattttcctt ctcttccttg atctcctcgt tcttctttgc agagatggtc
    13561 agacgatcat ttacaaggtc tatatcgata tcttccttgt tataacccgg cagctcagcc
    13621 tctaccacat actccttttc ggtctcctta atgtccgccc ggaatccgcc cgtcataccc
    13681 ccggaaagca tatcgttgcc aaagaagtcg tccattagat ccctgccgaa aaagcctttg
    13741 aataggtcga ttggtgttaa accgtttctc cttctgaatg gaaccatatc cttcattaaa
    13801 aactctccct tctttttggc ttgattttat ttttagtatt ccctgtatgt ttgagtatat
    13861 tatattctat ag
//
LOCUS       DASRNX010000220         5841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_468426,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000220 DASRNX010000000
VERSION     DASRNX010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468426"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1390)
                     /locus_tag="VFD89_08650"
     CDS             complement(101..1390)
                     /locus_tag="VFD89_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HZJ58283.1"
                     /translation="MDKLFAGLYGQDAEIISYQKKRYSEIFHRFNELFPDSAGHVQFF
                     STPGRTEVGGNHTDHNHGRVLAAGVTMDSVAAAAKTEDSLITLYSHGYDGVFQVDTHD
                     LVARDDERETTHALIRGIATRFKELGLRVGGFNAYVSSDVLRGSGLSSSASIEVLIGT
                     ILSHLYNDGQVDVKLLAIIGQYAENVFFGKPCGLMDQMACAVGGFVTMDFKDPNDPKV
                     EKIDFDFHSKGYSLLVVDTGGSHADLTEDYASIPIEMKAIAEALGAEVCRELTMKQVI
                     DNIPMLRERVNDRAILRAMHFIKENNRVTKQVRALEEDDFTKFLSLIKESGNSSWKWL
                     QNCYSAHAPLEQGVTLALALTEDFIGEIGKGACRVHGGGFAGTIQVFIPTGSVKGYIE
                     EMEPIFGEGSVTVLGIRPLGTTRVNIDIAKIYRLGKI"
     gene            1872..3455
                     /locus_tag="VFD89_08655"
     CDS             1872..3455
                     /locus_tag="VFD89_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HZJ58284.1"
                     /translation="MTHTKAKAAKLVEQLLAFGLFNEMIRDEDITFLRNQLLDLLNIP
                     EPHNDPNDPFDDGIPPTATPILEKLLDYAVEEGIIPDDTMVYRDLLDTKIMGIITPLP
                     SQVIDRFTSIKEKNGVQEATDDFYKLCQKSDYIRVDRIAKNLEWDYESSYGNLKVTVN
                     LTKPEKDPREIAALKNAPQVGYPKCLLCPENVGYSGRINHPARQTHRTLPVELGDETW
                     YFQYSPYVYYNQHCIVLSQNHVPMRLTRNTFDRLFGFLDQFPHYFIGSNADLPIVGGS
                     ILNHDHFQGGYYTFPMEVSPIEYFLNLGDFPEVKAGILNWPMSTLRLECADSKILTDL
                     ADRILSSWRGYSDPDAEILSESIDDNGETILHNTVTPIARMSQEGLYQLDIVFRNNRT
                     SSEFPLGIFHPHPDIHHIKKENIGLIEVMGLFILPGRLQKELSAIEDILTGKGTQNLE
                     GLVSDTHPLRLHSEWIYELIKLHGKNNSKEDAGAILEKAVGKKCQKVLEDAGVFKTTM
                     AGREAFNRFLTKLGFTVSD"
     gene            complement(3447..4694)
                     /locus_tag="VFD89_08660"
     CDS             complement(3447..4694)
                     /locus_tag="VFD89_08660"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZJ58285.1"
                     /translation="MNQDWRRDSDIIEGEYEIIGEWDAEEPPKDPGRNGIELGSGPPY
                     VTYALLGINIGVWVLMSLTGLFMGWGQGRQLLFFGAKVNVLVAQGEYWRLFTAMFLHI
                     GLLHLFFNSYALYIYGPAVEKLFGKLKFILVYIISGLVGSLLSYLFSPNDAAGASGAI
                     FGLMGSLLYFRKEKRSLFQRVFGPGLLMVIGINLLYGFIQPGIDNWGHIGGLLGGFLM
                     GNGLGLYREKDRKYGRRILFWAVTILIFVLGLGLGRLKYGPSIHLSRAYDAAREGHIL
                     DAKNHIERLSDKDRNGPKARELIEAVFIEDINNSMQEGHPDKALESMDVLLGYYPEEV
                     NYYFYRGNIYESMGDHERALGDYLHITEMVKGSEKVWFSAGRAAYNSGRISDAKMYLK
                     EALKINPNYYDAKKFLEDISDLV"
     gene            complement(4725..5786)
                     /locus_tag="VFD89_08665"
     CDS             complement(4725..5786)
                     /locus_tag="VFD89_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HZJ58286.1"
                     /translation="MLYPLLFNPIFKQRIWGGQRIGEIFDRQLPSHDIGESWDVASHE
                     NGTSVVANGVHAGKGLDELIGEYGRELMGTALDEKDIEKFPLLIKILDASGLLSVQVH
                     PGDEYATLHENGELGKTEMWYIIDAKPGAYLYYGVKPGVGPDEFRDAVQKGDVEPYLY
                     PLRVQKGDVLYIPAGMVHAIGEGIVICEIQQNSDTTYRVFDWNRTDEDGKPRELHIDK
                     ALDVIDFEGRYLKEKVKGIRVKVDGGFKTYFAAGPYFAMEELEIDEEVAETAHGDKFF
                     ILTAIAGRGSIDYDGGSQPFKAGDSIMIPACLGEYTICGNVTLLKTYIPHREKDIISV
                     LKAEGFSHKDMGAIAGLFD"
BASE COUNT         1569 a         1516 c         1192 g         1564 t
ORIGIN      
        1 atattctata ggtcaaagta agtcaaagta actatatggt caaaaatata gaatagacgc
       61 ctctatagcc tattctatga aattatccaa cttattctaa ttatatcttg cccaatctgt
      121 aaattttggc gatatctata tttacccttg tggttcccaa aggcctgatc cctaaaacgg
      181 ttaccgaacc ctcgccaaag ataggttcca tttcttctat gtaacctttg acgctcccgg
      241 tgggaataaa tacttggatt gtcccggcga atccgccccc atggaccctg caggcccctt
      301 ttcctatttc ccctataaaa tcttccgtaa gggcaagggc caatgttacg ccctgttcca
      361 acggggcatg ggcggaataa cagttttgaa gccacttcca ggaactgttt cccgactcct
      421 tgatgaggga caaaaatttc gtaaaatcat cttcctccag ggctctcacc tgctttgtaa
      481 ccctgttgtt ctcctttata aaatgcatag ccctcaaaat agccctgtcg ttaacccgct
      541 ccctcagcat cgggatatta tctattacct gtttcatggt caattccctg catacctctg
      601 cgcccaaggc ctcggctatc gctttcatct caatgggaat ggatgcataa tcttccgtta
      661 gatccgcatg actgcctccg gtatccacta ccaataggct atacccctta ctatggaaat
      721 caaagtcgat cttttcaacc ttcggatcat tggggtcctt aaaatccatg gtgacaaatc
      781 cccccacggc acatgccatc tgatccatga ggccacatgg cttcccaaag aatacgtttt
      841 ccgcatattg gcctattatg gccaaaagct ttacatcaac ctgtccgtca ttatagaggt
      901 ggcttaaaat ggttcctatc agcacctcga ttgaagccga tgagctgagc ccagaccccc
      961 taaggacatc gctggagacg tatgcattaa agcccccaac ccgtaaaccc aattctttaa
     1021 atctggtcgc aatccccctt ataagggcat gggtggtttc tctctcatca tcccttgcta
     1081 caagatcatg ggtgtccacc tggaagaccc catcataccc gtgggaatat agggttatca
     1141 atgaatcctc agtcttggcc gcagccgcta cggaatccat ggttaccccg gcagctagca
     1201 cccgcccgtg attatgatcg gtgtggtttc cccccacctc agtacggcct ggggtgctaa
     1261 aaaattgaac gtggcctgca ctatctggga acagttcatt gaatctatgg aaaatctcag
     1321 agtaacgctt tttttgatag ctaatgattt cagcatcttg gccgtagagc ccagcaaata
     1381 atttatccat tccttcctcc ttatgcctta acctattgtg ctccattgaa aattaaaatt
     1441 cccaaccgtt ttaacggaac agtttttaaa acatcggaag caattgcatt ggcaattgca
     1501 acacacaggt ttcaacgagg cgggggattt atacccatct cctgttttac taatcggaac
     1561 tcgcctctta tagaatcggg agacatctta ccgactcgtt ataacggatt taaaaacaaa
     1621 aaatctaaaa ggttatacat atattggctt tattatagcc ccggcgacga attaatacaa
     1681 tacccaagca atgctgaggt gaataatcca aatttgggga ataatatccc agcccttaat
     1741 gatttaaggc taagaaccca aaaacatgct taagtataat aatgctgcag aatattggtt
     1801 gaatcagcca taaatagtcg atataatgaa tgtaagactt gattattaag gatggaaagg
     1861 atgaaactcc catgacccat accaaggcaa aggccgccaa actggttgaa caattactgg
     1921 ccttcgggct ttttaacgag atgataaggg acgaggatat aacatttctt cgaaaccagc
     1981 tgctggatct gttgaatatc cccgaaccgc ataacgatcc caacgacccc tttgacgatg
     2041 ggataccccc taccgcaacc cccatcttag aaaagctttt ggattatgcg gtagaggaag
     2101 gcattattcc cgatgatacc atggtttacc gggatctgct tgatacgaag attatgggca
     2161 tcattacccc cctgccttct caggtaatcg acagatttac ttcaatcaaa gaaaaaaacg
     2221 gggtacagga agccaccgat gacttttata agctgtgcca gaaatccgat tatatcaggg
     2281 tggacaggat agcaaagaac ctggaatggg attacgaaag cagctacgga aacttaaagg
     2341 ttaccgtcaa cctgacaaag cctgaaaagg atcccaggga aattgcggca cttaaaaatg
     2401 cgccccaggt gggctatccc aaatgtcttt tatgtccgga aaacgtaggg tactccggcc
     2461 gaatcaatca tcccgcccga caaacccata ggacattacc ggtagagctg ggggacgaaa
     2521 catggtattt ccagtactcc ccttatgtat attacaacca gcactgcata gtgctcagcc
     2581 aaaaccatgt ccccatgaga cttacccgca acacatttga caggctcttt ggctttttgg
     2641 accagtttcc ccactacttt atcggctcca atgccgatct tcccatagtc gggggctcaa
     2701 tactcaacca tgaccatttt cagggcggat attatacctt tcccatggag gtttccccta
     2761 ttgagtattt cctgaatttg ggggattttc ccgaggttaa ggcgggtatc ttgaattggc
     2821 ccatgtctac cctaaggctt gaatgtgcag attctaagat ccttacagat ttggctgata
     2881 ggatacttag tagctggagg ggttattccg atcccgatgc agaaatactg tccgaatcca
     2941 ttgatgataa tggcgagaca atcctccata acaccgtaac ccctatcgct aggatgagcc
     3001 aggaaggcct ttatcagttg gatatcgtat ttcgaaacaa caggaccagc tccgaatttc
     3061 cccttgggat ttttcatccc catccggata ttcatcatat caaaaaagaa aacataggtt
     3121 tgattgaggt aatgggcctt ttcatactgc ccggccgcct gcaaaaagaa ttgtccgcca
     3181 tagaggatat attgacgggc aagggtacac agaatttgga ggggctggta tctgatacac
     3241 atcccctgcg ccttcacagc gaatggatat atgaactaat aaaattgcat gggaagaata
     3301 attctaaaga ggatgcggga gccatcctgg aaaaggctgt gggtaaaaag tgtcagaagg
     3361 tcttagagga cgccggcgtg ttcaaaacca ccatggcagg cagggaggcc tttaatagat
     3421 ttttaaccaa gttggggttc accgtttcag actaaatccg atatatcctc tagaaacttt
     3481 ttggcatcgt agtaattggg gttaattttt agagcctctt ttagatacat cttagcgtcg
     3541 gatattctcc cggaattata ggccgccctt ccggcgctaa accatacctt ttccgaaccc
     3601 ttcaccatct ctgttatatg aagataatcg cctagggccc tttcatgatc ccccatggac
     3661 tcgtagatat ttcccctgta aaagtagtag tttacctctt cgggataata accgaggagt
     3721 acgtccatag attccaaggc cttatccgga tgtccctctt gcatgctgtt gtttatgtcc
     3781 tctataaaga ccgcttctat aagctctctg gccttgggtc cgtttcggtc cttgtccgag
     3841 agcctttcta tatgattttt ggcatctaaa atatgacctt ccctggcagc atcataggcc
     3901 cggctgagat gaatcgacgg gccgtatttt agcctgccca ggccaagccc aagtacgaat
     3961 atgaggatgg taacagccca gaatagaatc ctcctaccat acttcctatc cttttcacga
     4021 tataggccca gcccgttacc cattaaaaaa ccgcccagca atccgcctat atgtccccag
     4081 ttgtctatgc cgggctggat aaacccatac aacagattta tgccgataac cataagtagc
     4141 cccggaccga atacccgctg aaacaggctc ctcttttcct ttctgaaata tagcaaagaa
     4201 cccataagcc cgaatatagc ccccgaggca cctgcggcat cgtttggact gaatagatag
     4261 ctgagaaggc tgccgacaag gccggagatt atgtatacca gaatgaactt aagtttccca
     4321 aagagcttct ccaccgcggg cccgtatatg tacagggcgt aggaattaaa gaacaggtga
     4381 agcaaaccta tatgcaaaaa catggcagta aacaatcgcc agtactcgcc ctgggctacc
     4441 agcacattga cctttgcgcc gaaaaacaac aactgacggc cctggcccca tcccataaag
     4501 agtccggtca gagacatcaa tacccagacc cctatattta tgcccagcag ggcatacgtg
     4561 acatatggag gaccggagcc taattcaatg ccatttcttc cgggatcttt tgggggttct
     4621 tctgcgtccc attccccgat tatttcatat tcaccctcta tgatatctga gtccctcctc
     4681 cagtcttgat tcatagaatc cttctatcct ttcataaatg gattttagtc gaacaacccc
     4741 gcaatagccc ccatatcctt atgggaaaaa ccctcggcct tgaggacaga aataatatcc
     4801 ttttccctat ggggtatgta ggtcttcagc agggtaacat tgccgcaaat ggtgtattcc
     4861 cccaagcatg cgggtatcat gatagaatcc cccgccttaa agggctggga gccgccgtca
     4921 taatcgatag agcccctacc cgctatagca gtcaggataa agaatttatc cccgtgggct
     4981 gtctcggcca cctcttcgtc tatctccagt tcctccatag caaagtacgg tccggctgcg
     5041 aaataggtct taaaaccgcc atccactttg acccttatgc cctttacctt ctcctttagg
     5101 tatcggcctt caaaatctat cacatccaga gccttgtcta tatgaagctc ccttggtttg
     5161 ccgtcctcgt ctgttctgtt ccaatcgaat accctgtatg tagtatcaga gttttgttgg
     5221 atctcacaaa tcacgattcc ctcaccgatg gcatggacca tgcctgcagg gatgtatagg
     5281 acatcgccct tttgtaccct tagtggatat aggtagggtt cgacatcccc tttttggacg
     5341 gcatcccgga attcatctgg gcccactccc ggttttacgc catagtatag ataggcacca
     5401 ggcttcgcat ctattatata ccacatttcc gtcttcccta actcaccatt ttcatggagg
     5461 gtcgcatatt cgtcccccgg atgaacctgg actgacagga gtccggatgc atccagtatc
     5521 tttatgagca gggggaactt ttctatgtcc ttttcatcaa gagcagtacc cataagctcc
     5581 ctgccatatt ccccgattag ttcatccagg cctttaccgg catgtacgcc gtttgctact
     5641 acgctggttc cgttctcatg gctggccaca tcccaactct ccccgatatc atgggacgga
     5701 agctgcctgt cgaaaatttc tccgattctc tgtccccccc aaatcctttg cttaaatatg
     5761 ggattgaaca ataatggata taacaaaaaa tcaccccaat ataattatga ttctaccgcc
     5821 ttgattatat cccggcagca a
//
LOCUS       DASRNX010000221         9009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_785291,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000221 DASRNX010000000
VERSION     DASRNX010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785291"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1292
                     /locus_tag="VFD89_08670"
     CDS             60..1292
                     /locus_tag="VFD89_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HZJ58287.1"
                     /translation="MDDNRKYDLLLKNGHVIDTGNKTNGKMDVAVKDGSIACVAPEIN
                     SELAEKVIDVKGNYVIPGIIDIHTHVYPLFPYQGLPNINADDHLLKAGCTTTVDAGSV
                     GWRDFLYFKESVIDKSVCRVLAYLNIAAQGMVDMKSEQYCKNMHPGVVASVIQAFPKD
                     LVGVKSAHYRPGGRPAYDAENPPWGSVDKALEAGELSGTHCMIDFGVCPKDSPYDEFV
                     TKKLRPGDIHTHVFAQQFPTVDENGKVYDYMWEARERGVLFDVGHGGGSFWFRNGHRA
                     LRDGFPPDIISTDLHAGSISGAAQSMLHTMGKFLNMGMPLEEVIACSTHIPAKVINRP
                     DLGNFSVGSCGDIAVISKIDGDFDYIDNGNAKIKGTSKFDCRMTIREGKIVFDGYGMS
                     MPLWEEAPKETYWREYWR"
     gene            1402..2523
                     /locus_tag="VFD89_08675"
     CDS             1402..2523
                     /locus_tag="VFD89_08675"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZJ58288.1"
                     /translation="MGIYDELGVKCIINGAATLTALGGSIMPPEVLEAMTEASKSYVP
                     IIELQHKVGAQIAKWTNNEAAMVSNGAAAGMVLATAACIAGDDEDKRRALPHTEGMKN
                     EVLVYGPQRVGYDFAIHQAGGKIVEYGSPDGTTEDQLEAAITDKTVGIFVFYFEHRMD
                     NQLPLEKVVEIAHKRDIPVIVDAAAQLPRRENLWRFTRDLGADVALFSGGKGLCGPQS
                     SGLVVGKKEYIDRMITFSCPNPGIGRPMKVGKEEIIGLMTAVKLYMEKDMEAEMECWE
                     AQVQSVLDAFRDESAVCASRDFPSEAGQPMPRAKVSMVEGALNADITQIDNWLKEGQP
                     SIFLAVMGNALYINPQTLQPGEMDIIIQKIKEAMDKYKK"
     gene            2535..2999
                     /locus_tag="VFD89_08680"
     CDS             2535..2999
                     /locus_tag="VFD89_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZJ58289.1"
                     /translation="MNAEKRLEEMGIDLKPVNRNGKSVVPIRRYKDLLYISGHGPVDE
                     NGDPLMQGCVGVDLDADQAYEAARLCAISMLRTIKDYLGDLDRVEEWVKVLGLVNSGG
                     DFWGMPRAINGFSDVMVKVFGERGRHARAAMGSANHESSYPVVVDAIIRIRD"
     gene            3191..3676
                     /locus_tag="VFD89_08685"
     CDS             3191..3676
                     /locus_tag="VFD89_08685"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZJ58290.1"
                     /translation="MGKIDINKKLTELTAREIEIPYVDYTGRTLVGARESNGLVYVSG
                     TGCEDPNDGHGVWTGAIGREVSLEEGYKAAQWCALLQLNIMRNQYGLDRIDSVVRAFA
                     LLQVADDFYDLDKVFDGYSDVMYGVFRDRGRHVRTIMGTRNLPNHLITVEVETIFKLK
                     D"
     gene            3828..4295
                     /locus_tag="VFD89_08690"
     CDS             3828..4295
                     /locus_tag="VFD89_08690"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZJ58291.1"
                     /translation="MNSAEDRLKELEIPFPDVNLKGRGVAPYKVVDDLLYIGGVSPLN
                     EEGQLAFEGRLGADLTEEEGYLAGRLVGLNMLQLIKDALGDLDKVDYVVRVIAMVSGK
                     KGFGEIYKAADGLSDLLTEVLGERGLHARNAVGASTLNGNAPIICDAIIKIRK"
     gene            complement(4345..4566)
                     /locus_tag="VFD89_08695"
     CDS             complement(4345..4566)
                     /locus_tag="VFD89_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58292.1"
                     /translation="MARKMLGWFIGLQPSIYLCYIGIRAHGQWAHNINLFMPVQQVLP
                     IGIYGQAAPAAKSNAMKLHIPMTITTPMD"
     gene            4794..5606
                     /locus_tag="VFD89_08700"
     CDS             4794..5606
                     /locus_tag="VFD89_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZJ58293.1"
                     /translation="MQYDRMLQDKHILITAGASQIGKACALLFASHGGIVAIADNDAE
                     AGDSLLYELQRFHKDSRFYYTDLTCKASIKDTCSRYLSDFGAPDVWLNCAGIYYPAFI
                     DEQEEASLNEMISLNTLSAFRVMKCLAAPMKEKRKGAIIQMVSDYAVTGANGVSVYGA
                     TKGGLLAMVNSFAMDYAPYGIRVNSILPGISIASMGDRITDEMGHEAAEDYWMRSQLL
                     PRRGSVQEVADTALFLASDMAKLITAEGLFVNGGQNAIAHSQYHRKKWGAHE"
     gene            5603..6349
                     /locus_tag="VFD89_08705"
     CDS             5603..6349
                     /locus_tag="VFD89_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZJ58294.1"
                     /translation="MKNYANLLEGKYAVVTNGGDPGGSAIVKKFAMHGAAVAFGVKDP
                     KTGDRILAEISDHSPQSFYMVLDLAVSEEIEEFCREVKRRFPLTTVLVNNPYGDISKT
                     ITGSDEEDDIILLQINQRSVMQTLRAFYGPMRDNRCGSVINISSNTVFKPVSGNPFLT
                     LSMGTMGGLTRTPAFEGGEFYVRINEILSGIRPGQAELSQCPLKVAGSHANGMADVVL
                     FLASDMSSYITGQSLTVDGGARRQLFKRQS"
     gene            6428..7156
                     /locus_tag="VFD89_08710"
     CDS             6428..7156
                     /locus_tag="VFD89_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZJ58295.1"
                     /translation="MDYQRLLAGRSAIVTGGAGSIGRAVAMLYASHGANVAILDEDEP
                     GIKTTLRDLLEINQGCFGFAGNLRDPESIVRLCNDAIDALGTADILVNAAGTYIEGDA
                     ETLTEDDFIFMIDKNLNSTIRFTKAIIPHMCDNRRGDIVNITSDLATASLAGTAGIAA
                     CAGAIAAFTRNVTMDYIRYHVRANCISFPLDGIKGREPLTGTPSTVDVANAALWYACK
                     MSRFIIGDMLPVNGGMDYFVGSEG"
     gene            7162..8022
                     /locus_tag="VFD89_08715"
     CDS             7162..8022
                     /locus_tag="VFD89_08715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZJ58296.1"
                     /translation="MKYNRLLEGKVAVITSGAHGMGKHVSVAYARHGAVCIINGRNPS
                     GVETGEMLNEISPGSCFIRCDMSKREDVESFIRQVLERTDRVDNVTNVVGINQSDLTD
                     QVVDERFEYTQQVNLYGIIRLARGFWPALQETRGNVVLISTIHSGPTYGKNSAYASSK
                     SATNAFARALAVEGAQYGIRGNVVCPGGIYSGFMREMYFKFKDDPEKLLQLGIRGQFG
                     HPDYGSGSSNDIANACLFLASDMAKHVTGATIMSDGGATFQSHEFRDIRIPPDYMELW
                     GKFMQDRYEL"
     gene            complement(8184..8750)
                     /locus_tag="VFD89_08720"
     CDS             complement(8184..8750)
                     /locus_tag="VFD89_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HZJ58297.1"
                     /translation="MTFFELLFISVGLSADAFAVAICKGLPIKKVNVKNAGIVGLYFG
                     GFQAIMPLIGYYLGVQFQGAIAAFDHWIAFILLGLLGINMIRESMSDKDDNVNEDKCL
                     DFNNMCALSIATSIDALAVGITFAFLQVNILPAVSFIGLVTFVLSVVGVKIGNVFGTR
                     YKSRAEFAGGLILIAMGIKILMEHTILS"
     gene            8828..>9009
                     /locus_tag="VFD89_08725"
     CDS             8828..>9009
                     /locus_tag="VFD89_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58298.1"
                     /translation="MKTCDYHMDMGPAETLDDAHLRIKIIKLLSLANTESCFVEAYIN
                     EMARTPYNNTTRQLYTA"
BASE COUNT         2606 a         1754 c         2357 g         2292 t
ORIGIN      
        1 attgaaagga gcgtgaacat agtaatcaag tcaaaataaa ttataaggag aattgcagga
       61 tggatgataa cagaaaatat gatttacttc ttaaaaacgg ccatgtgatt gataccggga
      121 acaaaacaaa cggaaaaatg gatgttgcag taaaagatgg aagtatagcc tgtgtcgcac
      181 cggaaataaa cagcgaattg gcggaaaaag tcatcgatgt caaaggcaat tatgtcattc
      241 ctggaattat cgacatacat acccatgttt acccactctt cccataccag gggcttccca
      301 atattaatgc ggatgatcat cttcttaaag ccgggtgcac gaccacggtt gatgcaggct
      361 ctgttggttg gcgtgatttt ctctatttca aagagagcgt aatcgataaa tctgtatgcc
      421 gtgtacttgc atatttgaat attgctgccc agggcatggt agatatgaaa tcggagcagt
      481 attgtaaaaa tatgcatcct ggagttgtag catccgttat acaggctttt cccaaggatt
      541 tggttggggt caaatcagca cactacaggc ccggaggcag gccggcctat gatgcggaaa
      601 atcccccttg gggctccgtt gacaaggcac tggaagccgg tgagctttcg gggacacatt
      661 gtatgataga tttcggtgtc tgcccgaagg attcccccta tgatgagttt gtaaccaaaa
      721 agctccgacc cggcgatata catacccatg tatttgccca acagtttccc accgttgatg
      781 aaaatggcaa ggtatatgat tacatgtggg aggccagaga gaggggggtg ctgtttgacg
      841 taggacatgg ggggggcagc ttttggttcc gtaacggtca tagggccctg cgtgacggat
      901 tcccgcccga tataatttca accgacttgc atgcgggcag cattagtggc gctgcacaaa
      961 gtatgttaca taccatgggc aaattcctta atatggggat gccccttgaa gaagtaattg
     1021 catgctccac ccatattccg gctaaggtta ttaaccgccc agatcttgga aacttttccg
     1081 ttggcagctg tggggatatc gcggtgattt ctaaaatcga tggagatttt gattacatcg
     1141 ataacggcaa tgccaagata aagggcacca gcaaatttga ttgcaggatg actattcgcg
     1201 aaggaaagat agtattcgac ggctacggaa tgtcaatgcc gctatgggaa gaagccccca
     1261 aggagacata ttggagggaa tattggcgat aggattcttc tattacagat aaacaattca
     1321 gggctataaa atttatcatc tgcagataaa tttaggttcc gtattgtttg tctataaact
     1381 atgcattagg gggaatttat tatgggtatt tatgatgaac tgggtgtaaa atgcattatt
     1441 aacggtgcgg ctaccttaac agccttagga ggctccatca tgcctccgga ggttttggaa
     1501 gctatgacgg aggcatctaa aagctatgtc cccataatag agctgcagca taaggtcggc
     1561 gcacaaatcg caaaatggac aaacaatgag gcggctatgg tatccaacgg agcagcggcg
     1621 ggtatggtcc tagctaccgc agcctgtatt gccggggatg atgaggataa gagaagggca
     1681 ttgccccata ccgagggtat gaaaaatgaa gtattggtgt acggtcccca aagggttggg
     1741 tacgattttg cgatacacca ggccggcggg aaaatcgtgg agtacggttc ccctgatggt
     1801 acaaccgaag accagctgga agctgccata acagataaga ccgtcggcat cttcgtattt
     1861 tattttgagc atcgtatgga caatcaactc cctcttgaaa aggtagtaga gatagcccat
     1921 aagagggaca ttcctgtcat tgtggatgcc gcagcacaac ttccgcgcag ggaaaacctg
     1981 tggaggttta cccgggattt aggtgccgat gttgcccttt ttagcggcgg taagggattg
     2041 tgcggtccac aaagcagtgg tctggttgtg gggaaaaaag agtacataga tcgaatgatt
     2101 acattttctt gccccaatcc gggcatcggc aggcccatga aagttggaaa ggaagaaatt
     2161 atcgggctga tgaccgcggt caaactatat atggaaaagg atatggaagc tgaaatggaa
     2221 tgctgggaag cccaggtcca atccgtattg gatgcgttta gggatgaaag cgctgtttgc
     2281 gcatctcggg attttcccag cgaagccggt cagcctatgc caagggcaaa agtttccatg
     2341 gttgaaggag ctttaaacgc cgatataaca caaatagata attggctaaa agagggccaa
     2401 ccaagcattt ttcttgctgt aatgggtaat gcattatata ttaacccaca aacattacaa
     2461 ccgggagaga tggatattat aatccaaaaa ataaaggaag ccatggataa atacaaaaaa
     2521 taggaggtat tttgatgaat gcggaaaaaa gacttgaaga aatggggatt gacttaaaac
     2581 cggtcaatcg caacggtaaa agtgtcgtgc cgattcgtag gtataaggat ttgctgtaca
     2641 tatcagggca tggcccggta gatgaaaacg gtgatccgct aatgcaaggt tgtgttgggg
     2701 tagacttgga tgcggaccag gcctatgagg cagcgcgtct atgcgccata agcatgctgc
     2761 ggaccataaa ggattattta ggcgatttgg atagggtcga agaatgggta aaggtgttgg
     2821 ggctggttaa ttcaggtggg gatttctggg gaatgccaag ggccatcaac gggttttctg
     2881 atgtaatggt gaaggtgttt ggtgaacggg gaagacatgc ccgcgcagcc atgggaagcg
     2941 caaaccatga atcgtcatac cccgtggtag tggatgctat cattcggata cgcgattaga
     3001 gaagccaaat gaggcggaaa gatgccgctt gcttttatag gtggcaggat ccgattagtt
     3061 aaagcacgag gtaggtacat aatcttcgca tcgttgaaac ctatgtgttg caattgacaa
     3121 tacaattgca tccgatgtta taaaaatata gaaaggagct tcgtttttat aaactaaaaa
     3181 cgaattaact atgggaaaga ttgatataaa taaaaaattg actgagctga cagccagaga
     3241 aattgaaatt ccctatgtgg attataccgg cagaaccctt gtaggggcac gggaaagcaa
     3301 cggtcttgta tatgtatcgg gcacgggctg cgaggatcct aatgatggac atggagtctg
     3361 gacaggggcc atagggagag aggtttcatt ggaagagggc tacaaggctg cccagtggtg
     3421 cgcgttgctc cagcttaata taatgagaaa tcaatatgga ttggacagaa tagattccgt
     3481 cgtacgggca tttgcacttt tgcaggtagc tgatgacttc tatgatttag ataaggtatt
     3541 tgacggctat tccgacgtta tgtatggtgt atttcgtgat cgtggacgtc atgttcggac
     3601 cattatgggg acccgtaacc tgccaaacca cctgataacg gtcgaagtag agaccatctt
     3661 taagcttaag gattaagatt agaaaattgg gaaggaaacc ggaggattag ccacaataat
     3721 gtgtcctgat aataggaaag gatatccatt aaaaggcgtc atcttaatat attgtggcca
     3781 tatcttcgat gatgggattt gaaaatatgt agatatggga ggaaattatg aatagtgcag
     3841 aagatagatt aaaagagctt gagattccat ttcctgatgt aaatttaaaa ggtaggggag
     3901 tagcacccta taaggtcgtt gatgatttgc tctatatcgg aggggtttca cccttgaatg
     3961 aggagggcca actggctttt gagggaaggc tgggggcgga tcttacggaa gaggaaggat
     4021 atttagccgg cagattagta ggccttaata tgctccaact tattaaagat gctttagggg
     4081 atctggataa ggtggactat gtcgtaaggg taatagcgat ggtgagcggt aaaaaaggat
     4141 ttggcgaaat ctataaggcg gcggatggtt tatcggattt attgacagaa gttttgggtg
     4201 aaaggggatt gcatgcacga aacgccgtag gcgcatccac attgaatggc aatgcaccca
     4261 tcatctgtga tgccataata aagattcgca aataatacat aatactagcg cgttgtgccg
     4321 gttatagacg gacaatacca gagcttaatc cattggcgta gtaattgtca tgggaatgtg
     4381 caatttcatg gcgttggatt ttgcggctgg ggctgcttgt ccgtagatac ctattggtag
     4441 cacctgttga accggcataa aaaggttaat attatgagcc cactgtccat gggccctgat
     4501 gccgatataa cataaataaa tgcttggttg caagccgatg aaccaaccaa gcatttttct
     4561 tgccatggag cgggaaatcc ccctttaatt ggcgtgatat tcggcaacca ggacagtata
     4621 acccaatttg acggataaag gggccgtcga taaattcaca aaatgggggg ggtatcccgg
     4681 tgaatgcgaa atcatgcccg tggggccatg ggaggtgcaa gccgagagcc gtcatatcct
     4741 atgggggtgg atgttaccgt ccgaatacgt gattaatggg agaggtgagc gttttgcaat
     4801 atgaccgaat gttacaggat aaacatattt tgattactgc cggggcctca cagataggga
     4861 aagcgtgtgc tttgctcttc gcttcacatg gcggcatagt ggcaattgcg gacaacgatg
     4921 cggaggctgg ggacagtcta ttatatgagc ttcagcgttt tcacaaggat agcaggtttt
     4981 attatacaga cctaacctgt aaggcctcca taaaggatac atgcagcagg tatctgtcag
     5041 attttggcgc cccggatgtg tggcttaatt gtgcgggaat atactatccg gcatttattg
     5101 atgagcagga agaggcatcc ttaaatgaga tgataagcct gaatacctta tcagcgtttc
     5161 gggtcatgaa atgccttgct gctcctatga aagaaaagcg caagggggca ataatacaaa
     5221 tggtcagcga ctacgctgta acaggcgcca acggagtatc ggtctatgga gccacaaaag
     5281 gcggtctgct tgctatggtc aattcatttg ccatggatta tgcgccctac ggtatacgcg
     5341 taaacagcat cctgcccggc atcagtattg cgtccatggg ggatagaata acagatgaaa
     5401 tggggcatga ggcagccgag gattattgga tgcgcagcca actgctgccc agacgcggta
     5461 gtgtgcaaga ggtcgcagac accgcacttt ttcttgcatc ggatatggcc aagctcatta
     5521 cggcagaggg cctttttgta aatggcggac aaaatgccat agctcatagc caatatcatc
     5581 gcaaaaaatg gggggctcat gaatgaagaa ttacgcaaat cttttggaag gtaaatatgc
     5641 ggttgtcacc aacgggggag atccgggcgg cagcgcaatt gtaaagaagt ttgcgatgca
     5701 cggtgcagca gttgctttcg gtgtaaaaga tcccaagaca ggcgatagaa tccttgccga
     5761 aatctctgat cattccccac aaagcttcta tatggtcctc gaccttgcgg tcagcgagga
     5821 aatagaagaa ttctgccgtg aagtaaaaag acgcttcccc ttgacaacgg tactggtaaa
     5881 caatccatac ggcgatatat ccaagactat aacgggttcc gacgaggaag acgatattat
     5941 tttactccaa atcaatcaaa ggagcgtaat gcagacctta cgagccttct atgggcctat
     6001 gagggataat agatgcggtt cggtgatcaa tatatcatcc aatacagtat ttaaacctgt
     6061 ctccggaaac ccctttctga ccctgagtat gggcacaatg gggggattga ccagaactcc
     6121 cgcctttgag ggaggcgaat tctatgtgcg aataaatgaa attctttcgg gaatacgccc
     6181 aggacaggcc gagttatccc aatgtccctt gaaggttgcg gggtcacacg cgaatggaat
     6241 ggcggatgtg gtactatttt tagccagcga catgtcctcg tatatcacgg gccaatccct
     6301 tacggtggac ggtggagccc gcagacaatt gtttaaaagg caaagttaaa atatcatatc
     6361 ttgcaaatca ttttgcacaa attgaggttg aaatatctac aagctaagac cgtgaggagg
     6421 gggattaatg gattatcaac gcttacttgc aggccgttct gcaatagtta ccggcggcgc
     6481 cggcagcatc ggtcgcgctg tcgctatgct gtatgcttcc catggtgcca atgttgccat
     6541 attggatgaa gacgaaccag gaattaagac aactctaagg gatttactcg aaataaacca
     6601 aggatgtttt gggtttgcgg gtaatcttag ggatccggaa tctattgttc gtctgtgtaa
     6661 cgatgcaatt gatgcattgg gtacagcgga tattttggtc aatgccgccg ggacttatat
     6721 agagggtgat gcggaaaccc taacagagga cgatttcatc ttcatgatcg ataaaaatct
     6781 taattccaca atacgtttta caaaagcgat tattccacac atgtgtgaca acagaagagg
     6841 agacatcgtt aacattactt ccgacctggc gaccgcttcc ttggcgggga cagcaggtat
     6901 tgcggcatgc gccggtgcca ttgctgcatt tacccgaaat gttacaatgg attatataag
     6961 gtatcatgta cgggcaaatt gcatctcctt tccattggac ggaataaaag ggcgggagcc
     7021 gttgaccggt acgccaagca ccgttgatgt ggctaatgcc gccttatggt acgcttgtaa
     7081 gatgtcgcgt tttataatcg gggatatgct gccggtaaac ggcggtatgg attattttgt
     7141 tggaagtgag gggtgaataa tttgaaatac aacaggcttt tagagggcaa ggttgcggta
     7201 atcacttccg gtgcccatgg catgggcaag catgtttcgg tcgcatatgc caggcatgga
     7261 gcagtctgta ttattaacgg gagaaatcct tcaggcgttg aaaccggaga aatgttgaat
     7321 gagatttcac ccggatcctg ttttatcagg tgcgatatgt cgaaaaggga agacgttgaa
     7381 tcctttataa gacaggtgct ggaacgcacc gatcgggttg ataatgtgac caatgtggtg
     7441 ggcataaacc aaagtgatct gacggatcag gttgtcgatg aacgatttga atatacacaa
     7501 caagtaaacc tgtatggtat cattcgttta gctcggggat tttggcccgc gcttcaggaa
     7561 accaggggta atgtggtgct tatttcaacc attcattctg ggcctacata tggtaaaaac
     7621 agtgcatatg catcatctaa atcggctacg aacgcatttg cccgtgcatt ggcggttgag
     7681 ggagcgcaat acggtatacg tggcaacgtg gtatgcccgg gaggcatata ctccggtttt
     7741 atgcgggaga tgtatttcaa atttaaagat gatccagaga agcttttgca acttggaata
     7801 aggggccagt ttggacaccc ggattatggc agcggaagtt ccaatgacat agcaaacgcc
     7861 tgcttgttcc tggcatcgga tatggctaaa catgtgaccg gcgctaccat aatgtcggac
     7921 ggcggggcta ctttccaatc acatgaattt cgggacatcc gtattccccc ggactatatg
     7981 gagctttggg gcaaatttat gcaggacaga tatgagctgt gatttcacag aaaagagcat
     8041 aataaatgtt atgaggcggc gatccgtaca ggggccgcct tttttgatcg tgattatttg
     8101 ggattttccc gctaaatatc cagcgtatct aaataggagt aaaaaaataa tatgaaacag
     8161 tttgtgtggg caagggttat aagctaggat agaatagtgt gttccatgag aattttaatc
     8221 cccatagcta tcaatataag gccgccggca aattcagccc tggacttata tcttgtgccg
     8281 aaaacattac ctatctttac ccctaccacg gacaatacaa aagtgaccag tccaataaag
     8341 gagaccgcag gcaatatatt aacttgcagg aatgcaaagg ttatgcccac ggctaaggcg
     8401 tctatgctgg tagcaatgga tagtgcacac atattgttaa aatccaagca tttgtcttca
     8461 tttacgttgt catccttatc cgacatggat tcccttatca tattgatacc cagaagtccc
     8521 aacaaaataa aagctatcca atggtcaaaa gcagcgatag ctccttggaa ctgaacccca
     8581 aggtaatatc ctataagggg cattatagct tggaatcccc cgaaatatag tcctacaata
     8641 cctgcatttt tcacatttac cttctttatt ggcagaccct tacaaatagc gacagcaaat
     8701 gcatcggcag ataaaccaac agagataaac agcaattcaa aaaaggtcaa tagaagttct
     8761 ccttatgtat acttgtccct acactgtata agtatatatg gtaggggatg tataggtcaa
     8821 ccgtttcgtg aaaacctgcg attaccatat ggatatgggc ccggctgaaa cccttgatga
     8881 tgcacatctc aggataaaga ttattaaact cctgtcatta gccaatactg aaagttgttt
     8941 tgtagaagca tatataaatg agatggcaag aacgccgtac aataatacaa ccagacagct
     9001 atacacagc
//
LOCUS       DASRNX010000222         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_574571,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000222 DASRNX010000000
VERSION     DASRNX010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574571"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            553..1341
                     /locus_tag="VFD89_08730"
     CDS             553..1341
                     /locus_tag="VFD89_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZJ58299.1"
                     /translation="MKRGVQTYTVRDFMGSREEVESSLRKIKEIGYDSVQTWTPSFMS
                     HAEHKALLDDIGLETCSSGGSYEQMLEDPAAIEEAVEQAKIYGVKYIGVGTLPRELRE
                     CESGYRQYAENINKIASQLKQENMKLLYHNHALEFFSLGGGLMGMDIILGDTDPDGLH
                     FALDTHWLTAGGVNVVDWIYKVKGRMGIIHFKDYAIVGGAMKIEDVHKNFAEVGEGNI
                     DWPNVVKACKDIGVEFAIVEQDICPGDPFDSLKISFDNMVKFGV"
     gene            1594..>2399
                     /locus_tag="VFD89_08735"
     CDS             1594..>2399
                     /locus_tag="VFD89_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZJ58300.1"
                     /translation="MDVVGIDTPCVDLLANIPKIPEANKGTRIQDYSWQGGGKVSSAM
                     VASARLGARCGMIGVVDGDAYGKFCLDDFKDHGIDVSRMIVDKDGETPFSIVLADDHT
                     HGRSIIYYPGTNRPLEVSDLDKDYIQSARYLHLAHDTPVTKQAAVWAREKGTRVVYDA
                     DGYRKGIEDMIPLIDVFIASEFFYEAMFKDGDYEANCRKVQDMGPDIVVFTLGEKGCV
                     GVEGSEFFMEEGFSVEVMDTTGAGDVYHGAFIAGLLQGWDARQTARFSNAV"
BASE COUNT          667 a          462 c          665 g          605 t
ORIGIN      
        1 catcgatgta gcgcagagct gggcttccat acctttgaag taatcaacgg tataatggag
       61 agctgtaaca cagggaaggt ttatgagatg acatccaagt gcgaacgtcc ggctcccttg
      121 ccctccggct ttatgagcgg gactgcggca gaggcttgcc tggataacta atttaggata
      181 gttgacaaat aggtacccaa agccctttat gcccatgctt aaaggggggt atctatacgg
      241 atctgaccgt caggatgcat atggtgatcc cgatgccgga ttgacggttg tgtccggttt
      301 ctatataata aatacaagat aataaacata agtgcttcaa tatcggtgcc actgcttatt
      361 tcacgactgg gcaggagacc aacccgtttt cacgggtagt ttaatccgtt gtcccggatc
      421 attgtcttgt tatatgacaa taatatgggg ttattgataa tcagtaccgg atgcagcgtt
      481 cgatagtcgt tacgatgtat ttaaccggcg cagcgggtca atttaaaata tagagaggtg
      541 gaaggatacg ttatgaaaag aggcgtacaa acttacacag tcagagattt tatgggcagc
      601 cgcgaagagg tggaatcatc gcttcgaaag atcaaggaga taggctacga ttcagtacag
      661 acttggacac ccagctttat gtcccacgca gagcataagg cgttgttgga tgatataggt
      721 ctggaaacct gttcgtctgg cggcagttac gaacagatgc ttgaagatcc tgcagccatt
      781 gaagaagcgg ttgagcaggc caagatttac ggcgtcaaat acattggagt cggaaccctg
      841 cccagggaac tgcgcgagtg cgagagcggt tataggcaat atgcggaaaa cataaacaag
      901 atcgcatccc agcttaagca ggagaacatg aagcttttat accacaacca tgcactggag
      961 tttttttctt taggcggcgg tttaatgggg atggacataa tcctgggtga tacggatccc
     1021 gatgggcttc attttgcact ggatacccat tggttgactg ccggtggtgt taatgtggtt
     1081 gactggatct ataaggtcaa aggccgtatg ggaatcatcc actttaagga ttatgctata
     1141 gtcggcggcg caatgaagat cgaagatgta cataagaact tcgcagaagt gggcgaggga
     1201 aatatcgatt ggcctaacgt tgtcaaagca tgcaaggaca tcggcgtaga atttgcaata
     1261 gtagaacagg atatatgccc cggagatccc ttcgacagct tgaaaataag ctttgataac
     1321 atggtaaaat tcggggtctg acagacgaca agctggctgt agtacaggga tgtccaacag
     1381 ggatatatcg tttacagcgc tataaaatga ttttgatatg cagattttta taacaccccg
     1441 gctgattatt tttaatgata ccggcaaaac aaagggcaat tgaaaataag acttttaatc
     1501 aaccaagccc ataattatac ccgttaaaat agggggcttg gttgattata tggagagcag
     1561 acttatcatt tattacgaaa ggtggaagtg tggatggatg tcgtaggaat tgatacacca
     1621 tgtgttgatc tcttagccaa tattcctaaa attcccgaag caaataaggg taccaggata
     1681 caggactaca gctggcaagg cggcggtaag gtatcttctg ctatggtggc ctcagccagg
     1741 ctgggcgcac gatgcggtat gataggcgtc gttgatgggg atgcctatgg gaagttctgt
     1801 ctggatgatt ttaaggatca tgggattgat gtatccagga tgattgtgga taaggacggg
     1861 gagacaccgt tttccatagt gctggccgat gatcataccc acggaaggag tattatctat
     1921 tatccgggaa ctaaccggcc tttggaagtt agcgatttgg acaaggatta tatacaatca
     1981 gctcgctatc ttcacttggc tcatgatacg ccggtaacga aacaagccgc tgtatgggcc
     2041 agggaaaagg gcacaagggt agtgtacgat gcggacggtt accggaaagg catcgaggat
     2101 atgatacccc tgatcgatgt ctttatcgcc tcggaattct tctatgaggc catgttcaag
     2161 gacggggact acgaagccaa ctgcaggaag gtacaggaca tgggacctga tatagtggtc
     2221 tttaccttgg gtgagaaagg ctgcgtaggg gtagagggca gtgaattttt catggaggaa
     2281 ggctttagcg tagaggtgat ggatacaacg ggcgcaggcg atgtatatca tggggccttc
     2341 atagccgggc ttctccaggg ctgggatgcc aggcagaccg cacgtttttc aaatgccgt
//
LOCUS       DASRNX010000223        26131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_362907,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000223 DASRNX010000000
VERSION     DASRNX010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 26131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26131
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362907"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VFD89_08740"
     CDS             complement(<1..1238)
                     /locus_tag="VFD89_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HZJ58301.1"
                     /translation="MDKKSFVKGAGILAVSGLVVKIIGAVFRIPLYNIIGPEGIGLYQ
                     MAYPIYAFLLVASTAGLPVAISKMVSEELATGNHYGGYRIFRISRRLLTLLGIATSVI
                     LLIGSGLISKIQGNPKSIYALMAISPALFFVSIMSAYRGYFQGMHVMHPTALSQIVEQ
                     LGKLIMGLTLAYLWLPRGPEFAAAGAIVGVTLSEVAALVLLLGIFRRERRAILHNARL
                     TYRSHYQAPSKDIILRLIEIAVPVTIGASIMPLVNMSDQVIVVNRLAPIVDKIANIPY
                     GLESFIEFAAKKGIEIPANPSLANIPLPMVDTYVEYIKMIATSLYGILTGGVNTLINF
                     PAVLTIALAMSLVPAISESYALKDMEGVRSKTTLGIRLTLLVGVPAAVGLGVLAEPIL
                     KSLYRMEEWEYIYGAQLLITL"
     gene            complement(1396..2913)
                     /locus_tag="VFD89_08745"
     CDS             complement(1396..2913)
                     /locus_tag="VFD89_08745"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZJ58302.1"
                     /translation="MPNKENVNEKFEKLMEEVGERKKRAKDSIRQDAHAEYLSSISPL
                     TTEEKKDVPNIIFILMDDLGWGDLSCFGSKAIHTPNLDRMAEEGLIMENCYSSSPVCS
                     PSRFGLMTGRYPSRGFIKNVFFPTVEIEERHMVVDRGYETPEELAGGRPSREAVERAR
                     QASKFIGGAIPVEGIMPDEVTVAEILQARGYRTGMFGKWHLGDKEPHLPNNKGFDYFY
                     GAHYSNDMVPYHFWRNEEIAVEAPFDQSKITEYLNTEFFKFIEDNSDGPFFAYYASPW
                     PHHPLNSGEKFKGTSKGGVYGDCIEEFDHGIGQLFDLLKEKGLLDNTFIAFSSDNGPW
                     HQGSPGGHRGRKGNAFDGGQVVPTICYWKDKVVNGTIREQAMNIDFLPTFSEMAGVEL
                     PKDRIIDGKSILSLLKGETKESPHDCLIYVDPGEYEDQGFGVRSQDNFKYYKATNSEN
                     ATYKNMMIHSFLFDLDHDRDESYDVKALYPEKYKELREHLDDFNESIKKNPRGWK"
     gene            complement(3044..5365)
                     /gene="yicI"
                     /locus_tag="VFD89_08750"
     CDS             complement(3044..5365)
                     /gene="yicI"
                     /locus_tag="VFD89_08750"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017689285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HZJ58303.1"
                     /translation="MKFTKGYWLMREDLDAYFPVHLHDLEMDEDSITAFAPPRPVEDR
                     GDTLNCKLLTAKFSSPMEDVIRVRFYNFKGGTAKTPEFEIFERDDTRVEISEDEDYAS
                     LTSGRLKVRINKRGRWGVDFIGDGKVITRSEDKAMGYIETHDGDTYVHEQLSLGVREN
                     VYGLGERFTAFVKNGQTVDIWNEDGGTSSEIAYKNVPFYMTNKGYGVFVNHPEKVSFE
                     IGSEKVSRVQFSVPGQYIEYFLIYGPEPKSILDKYTQLTGRPALPPAWSYGLWLTTSF
                     TTSYDEDTVNSFIDGMAERDIPLHVFHFDCFWMKEFHWCNFEWDERVFPDPEAMLKRL
                     KARGLKICLWINPYIAQRSKLFDIGKEKGYLLKRPNGDVWQWDLWQAGMAVVDFTNPE
                     AYEWYKSELRRLIDMGVDSFKTDFGERIPTDVVYHDGSDPERMHNYYPQIYNKAVFEV
                     LEEKLGEGEACLFARSATAGGQQFPVHWGGDCSANYESMAESLRGGLSLGMSGFGFWS
                     HDIGGFEHTATADVYKRWCAFGLLSSHSRLHGSTSYRVPWLFDEEAVDVLRYFTKLKS
                     TLMPYLYSASCEAVEKGTPVMRSMHLEFPEDPTCDYLDLQYMLGDSLLVAPVFSEEGK
                     ASYYLPRGNWTHFIDGRVMEGGQWIEEDYGYMSLPLWVRPGSVIPVGSNDERPDYDYS
                     RDISFHLFEMEDGDIIRVMVPDLDGSAGLNAAAYREGDEIRVEVDNPEKPWNVLLRNI
                     DSVASVEGGTMRETDEGLLITANRESSTVKIAL"
     gene            complement(5791..6348)
                     /gene="spoVT"
                     /locus_tag="VFD89_08755"
     CDS             complement(5791..6348)
                     /gene="spoVT"
                     /locus_tag="VFD89_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HZJ58304.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDRDGEV
                     ILKKYSPIGELSDFAQEYTESLHNTLGHIACITDKDFIIAVAGGSKREFHEKSISMDI
                     ERIMEGKKTMVANRSQNNTLFSIVSNGDDAGKYSSQVIVPIISQGDPIGTIILLSKET
                     DEDMGESEVKVAETAAGFLAKQMEH"
     gene            complement(6484..7665)
                     /locus_tag="VFD89_08760"
     CDS             complement(6484..7665)
                     /locus_tag="VFD89_08760"
                     /inference="COORDINATES: protein motif:HMM:NF025009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZJ58305.1"
                     /translation="MEHIRKISILLVIASMLGIMAGCGMVQVNPEKDRAQVVAQVDGE
                     EILKGEVLDQLDREKESHNLTDEGLRDKNNREHVMEIKKSILDQLAIQKLLDQKAAAA
                     GFTVTDQLMEETREDLADIKKSIQRQLEEADEENEDEESQEKDYVKEAEDYLDNYLAT
                     MGMTQEEYVAFLAEQKCIEKLYDKTVEGIEVSASDIEEFYNEELAKQKENTTFLEGRS
                     VDLFDPPKIRVKHALVEIPGEKVTEYRTLLNDKKEDEAKELLDKELEAIKDKAQKALD
                     KAKKDGDFDEMVGEVNPDSAEEMKEGITIHKENIYLPEEYKEAAFKLKVGGVSDLVAT
                     PNGYYIIKLEEKYSEKTYTLEEKRDDIKEILDRQEQDEKWKEVVDDWKEKLVKKFEKR
                     L"
     gene            complement(7715..11206)
                     /gene="mfd"
                     /locus_tag="VFD89_08765"
     CDS             complement(7715..11206)
                     /gene="mfd"
                     /locus_tag="VFD89_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZJ58306.1"
                     /translation="MTVKWLDPMTRWDEFIKMRQAAREGGGPILVFGLSESQKNHAVS
                     SLLYPLSRQCLYITYNHIQAKRVYDDLRFYMEDYVMLLPPRDIVLYDVAAHSTEVSGQ
                     RLKVLEALALGEYRVVVASIEALLCLQTPPEIFKGGLIRVDVGQVFSIRDLAYKMVEA
                     GYERVPAVEGPGQFSIRGGIVDVYPMTMDNPCRIEFFDDEVDSIRLFDPLSQRSTEKL
                     DGVSISPARELVLTAKSHARGLEGIKRDLAAATKKGKQGPKTRVSALLEDMEQGMQSR
                     NLENYFSFFYPENNSLFDYMSPESLVVLDEPSRMGESFSGWEEEFIEHFKTLLEDGEV
                     LPGQGEIFISYQELMAQSEKFHRLALQGLPKSVPGYSPRAIFNVASRGIPSYQGKLDI
                     LAEDLRYWRGKRYSVILFAGSGSRCQSLVSNLYDLGIEATFSPGAEGHIMPGQIMVLS
                     GNISGGFEYPQGKFAVISHNEIFGTMKHRRKSKAPSGKGKLDPFTDLKIGDFVVHEAH
                     GIGKYLGIETLVINDQKRDYLNIKYGGTDRLYIPTDQVDIIQPYIGMDQKEPRLSRLG
                     GAEWQRAKTKARQSVQKLAMDLVELYAARETTKGHQFSLDTPWQNQFEEMFPYEETPD
                     QERAITDIKKDMESGRVMDRLLCGDVGYGKTEVAIRAAFKAVMDGKQAALLAPTTILA
                     QQHFNTFVERFGDFPVKIQVLSRFRTPAQQRDILRALKQGNIDIIIGTHRLLGKDVVF
                     KDLGLLVVDEEQRFGVGHKETIKNIRKNLDVLTLTATPIPRTLHMSLVGIRDISTIET
                     PPEDRIPVQTYVVEYNDSLIRDAILKEVSRGGQVYFVYNRVRSMERMAERLRELVPQV
                     RIRMAHGQMGESLLENVMLDFYDGKFDLLLCSTIIESGLDIPRVNTIIVYDSDYYGLS
                     QLYQLRGRVGRSNRLAYAYLTYRKDKVLSEVAEKRLQAIKEFTEFGAGFKIAMRDLEI
                     RGSGNILGPQQHGHMTAVGYELYCKLLDEEIKSLKGEAVPKPLDITIDIRVNAYIDDD
                     YIPLEGQKIEIYKKIAAIEGLQDKYDIEEELEDRFGDMPSPVTNLIDIAYIKGLARGL
                     GISEVSHRDREVILRLTGSKTFDSRSLMVLLNENRGRLRYEGMRRPMFRIILRDDAPL
                     TALTETKDILEQMVKLG"
     gene            complement(11263..11823)
                     /gene="pth"
                     /locus_tag="VFD89_08770"
     CDS             complement(11263..11823)
                     /gene="pth"
                     /locus_tag="VFD89_08770"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZJ58307.1"
                     /translation="MYIIAGLGNPGSKYADTRHNIGFIALDMLAERLGIKVARIKHKA
                     LIGEGTFEGHRVVLAKPQTYMNLSGESILDIKNWYKVEDSKIIVIYDDIDLDVGVLRI
                     RSSGSAGTHKGMQSIIYQLQSQDFPRVRIGIGETPEGWDLKNYVLSKFKDDEFPPLKE
                     ACERAVKGVELIITKGVEYAMSKCNG"
     gene            complement(11835..12788)
                     /locus_tag="VFD89_08775"
     CDS             complement(11835..12788)
                     /locus_tag="VFD89_08775"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZJ58308.1"
                     /translation="MITHGTSIKIFSGNSNPKLAREIAEHVGIPMGNSEVSTFSDGEI
                     SVNIHETVRGADVFVVQSTCFPGNDNLMEMLIMIDAFRRASAGRITAVMPYYGYARQD
                     RKAKARDPITAKLIADLITTAGADRVLTMDLHAAQIQGFFSIPLDHLLGVPILTQYYL
                     EKGFEHEDLIVVSPDIGSVTRARNFANRLNAPIAIVDKRRPRANVAEVMNIIGDIKGK
                     KVLLVDDMTDTAGTLVQSALALLDGGAKEIYACATHGVLSGPAIERLQDSPIKEMVIT
                     DTIPLTPEKQIDKIHVLSVAPIFAEAIDRIYEDLPVSTLFE"
     gene            complement(12785..14170)
                     /gene="glmU"
                     /locus_tag="VFD89_08780"
     CDS             complement(12785..14170)
                     /gene="glmU"
                     /locus_tag="VFD89_08780"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZJ58309.1"
                     /translation="MENKISAIIMAAGYGPGMKSSLPKAMHKICGEPVVEFVVASVLD
                     VVNIRPLVILGEGSEGIGERLGTRVNYSHLPESAQMGHGAPLIKDYLKDDVGYVILMD
                     GNLPLLTPDTLKDLADSCQGGKYDAVVLSALSDKPQGYSRILRNISGDFEGILEDVKA
                     LDEENLIKEIDTSVYCFRVSALLDGLDRFGGKDTDHRHIIGLLNDLKASGGRIGLHTV
                     YDSTETMAVDTRVHLVEAQKIMGQRINSRHLERGVTIIDPDNTYIGPHVTIGRDAIIY
                     PGNFLEGHTAIGEGSILLPNNRILDSVIGKDTRIENSVILESHVGDKTTVGPFAYLRP
                     GSLIEENVRIGDFVEIKNAKVGRGSKVSHLTYVGDGEIGENVNIGCGVVFVNYDGKKK
                     HRTVVGDNAFVGCNTNLVAPVKVEDNSYIAAGSTITQDVPKNALAIARAKQVNKENWV
                     KSEENETEENQ"
     gene            complement(14426..14686)
                     /gene="spoVG"
                     /locus_tag="VFD89_08785"
     CDS             complement(14426..14686)
                     /gene="spoVG"
                     /locus_tag="VFD89_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HZJ58310.1"
                     /translation="MEITDVRVRKVGGDGKMKAVASVTFDNDFVVHDIKIIEGQSGMF
                     IAMPSRKTPDGEYRDIAHPINSDMRARLQGMILQQYDQIDEE"
     gene            complement(14888..15709)
                     /gene="purR"
                     /locus_tag="VFD89_08790"
     CDS             complement(14888..15709)
                     /gene="purR"
                     /locus_tag="VFD89_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HZJ58311.1"
                     /translation="MEKVRRNERIGAMVNILCNHPNKIFTLNHFSDMFKTAKSTISDD
                     VVIIRELLGRFQLGDVETVVGAAGGVRFVSRLFKDDEIFIKDICTTLSVPDRILPGGF
                     LYMTDILFMPGVVQRFGNILATQFYNDRPDFVITVETKGIPIALAAARALNCPVVMAR
                     RENKVTEGSTISINYVSASSRRIQTMSLVKRAVKKGQRGLIVDDFMKGGGTAKGLMEL
                     LHEFEAEIAGVGVIISTEDPGQKMVKNYKSLMVLKQVDEGERKVVVEPSPWINAL"
     gene            16033..16746
                     /locus_tag="VFD89_08795"
     CDS             16033..16746
                     /locus_tag="VFD89_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZJ58312.1"
                     /translation="MRKILGCLKRAVEDFNMIEEGDRIAVGVSGGKDSLVLLKALRLY
                     QYMSPVEYELEAITLDMGYDGTDWTGVKDFCGDLGIPYTIKDTNIAAIVFDVRREKNP
                     CSLCANLRRGALNNAALGLGCKKVALGHHREDVIETLFLSMFYEARLNTFSPVTYLDR
                     RDITLIRPMIYVPETDIKRAVSRHDIPVVFNPCPANGNTKRDYIKNLLHDISGEIPNV
                     HEQILGALRKTKVRNLWND"
     gene            complement(16754..17191)
                     /gene="fabZ"
                     /locus_tag="VFD89_08800"
     CDS             complement(16754..17191)
                     /gene="fabZ"
                     /locus_tag="VFD89_08800"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HZJ58313.1"
                     /translation="MDVCDINEIMERIPHRYPFLLVDRVLELVPGKSGKGIKNVTINE
                     PFFAGHFPGAPIMPGVLIVEAMAQLGAMVVLSLPENRGKLAFFAGIDSMRFRRQVIPG
                     DQLAMEVEITRMRGSIGKGNVKALVEGQVAAEGSIMFGLGDAT"
     gene            complement(17198..17980)
                     /locus_tag="VFD89_08805"
     CDS             complement(17198..17980)
                     /locus_tag="VFD89_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HZJ58314.1"
                     /translation="MIRALYTGTSGMRVQQHKVDSIGNNIANSQTVGYKRSRPDFKEA
                     LYNIIENRPEEAGPNPSVGNGTLVGSVQTHFTQGALHPTNVSLDLALDGPGFLTVESD
                     DGEYLYTRDGSLRLSHLPGGGTRLDNGDGYPVLDQYGQFFVFDSGTREEDISITEYGD
                     VYLNDQDGNATYGGRLGIYGFSDPNGLERINGSYFMANDDSGEPVGSMETKVFQGYLE
                     QSNVDMAREMTDLIIAQRAYQLNSRVVQTADEMEKQANSLRG"
     gene            complement(17991..18743)
                     /locus_tag="VFD89_08810"
     CDS             complement(17991..18743)
                     /locus_tag="VFD89_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HZJ58315.1"
                     /translation="MLRGLYTAATGMIHQRDKLNTIANNLSNTNTRGYKRDSVVSHSF
                     RDELLLRTGGQAGGVKPLGPLNHGVYVERTVPLFEDGALEQTDLITDIALQGNGFFTI
                     LRDGEEFYTRDGTFKVDDEGFLVTMEGHTVDGEYGPIHVGQGNFDIDDEGNIWQDDGF
                     VNSFRLVDFEDATMLDKVGDNLFVNNNPGANQALAFEGQVFQGYAEASNVELLEEMSN
                     MIITSRAYEANQRVLQMIDSTLSKTVNEVGSL"
     gene            complement(18963..19994)
                     /gene="mreB"
                     /locus_tag="VFD89_08815"
     CDS             complement(18963..19994)
                     /gene="mreB"
                     /locus_tag="VFD89_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HZJ58316.1"
                     /translation="MFANRDIGIDLGTSSVLIYMRGKGVVLREPSVVAIDLKGGKILA
                     VGSSASKMLGRTPGNIVALRPLQDGVISDYEITEKMLRYFFRRAIVRRIGRKPRVIVC
                     VPSGVTEVEKRAVIDASYEAGAKRTYLIEEAIAAAIGAGIDISKPYGNMVVDIGGGTT
                     DIAVISLGGAVVTDSIKIAGDMFDDSIVRYMRKNYNLLIGDRTAEKMKINIGSAIPRK
                     EDVVMDVTGRNLISGLPKTISVNSREMVEALEEPLETIAETIHSVLEKTPPELSSDIS
                     EIGITMTGGGSLLHGLDKLLEDRLKIGINVVEDALSCVAIGTGKALEDIDLYTDTPAF
                     NYRGGTLTY"
     gene            complement(20342..20623)
                     /gene="spoIIID"
                     /locus_tag="VFD89_08820"
     CDS             complement(20342..20623)
                     /gene="spoIIID"
                     /locus_tag="VFD89_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HZJ58317.1"
                     /translation="MKDYIEERVIEIACYIIETGATVRETAKVFKVSKSTVHKDVSER
                     LPKVNQTLWAGVKKVLDQNKAERHIRGGRATKIKYQLENEDTSHEENTE"
     gene            complement(21266..22174)
                     /locus_tag="VFD89_08825"
     CDS             complement(21266..22174)
                     /locus_tag="VFD89_08825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZJ58318.1"
                     /translation="MKSDKSRGRFRQAFTKEKVIRFIDKQGFYIVLFVCIAIIGVTAF
                     LTSGKNPKPPIQELADNEDVIKLPEPTKVDRLDTAGILEDKISIKIKDSSPPPEVDKS
                     EEGTLEGESKETLAPKGDKKEDETKNKDGVKDNDEAKGKGETKNEGVKKEEAPKEKKE
                     TPKKSTQKAQMIYPVQGNVSKGHIMDELVFSNTLKEWTTHSGMDIESFVGSEVKAAMG
                     GTVEEITEDRLMGICITLDHGNGLKTYYANLSTGNMVSVGQKVDKGQIISGVGRTASC
                     EILDDPHLHFEVMLDGKSLDPLAYLQ"
     gene            complement(22476..23501)
                     /gene="spoIID"
                     /locus_tag="VFD89_08830"
     CDS             complement(22476..23501)
                     /gene="spoIID"
                     /locus_tag="VFD89_08830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HZJ58319.1"
                     /translation="MRHWIDILLFIIVIVLILPSMVVGLFSTTDPDEPPPDFQVRSSG
                     DSIRVESNNVNYQEPTITLYNHYTKALEDMPLEEYVVGVVAAEMPSSFEIEALKAQAV
                     AARTLAARKLRSYGGSGCSKHKGADVCSEFIHCQAWIPESQQRKNWGDNYEENILKIR
                     RAIEETRGYIMTYMAKPIEVFFYSTSNGKTEDVAEVFSASLPYYQVVDSPGEEDAPRY
                     RGSVSLTNKEFVRIFNSKYTKSGLSSSNLEQKIKIVSRTDSGRVRDLKVGDTKVKATE
                     FRTLYGLNSTDFDFRFEGDKVHIETTGYGHGVGMSQIGANAMARQGYNYSDILGHYYR
                     GIKIETI"
     gene            complement(23797..25047)
                     /gene="murA"
                     /locus_tag="VFD89_08835"
     CDS             complement(23797..25047)
                     /gene="murA"
                     /locus_tag="VFD89_08835"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZJ58320.1"
                     /translation="MAKLIVKQSPPLTGKVRIHGAKNSVLPIMAASLLADDICTLEEV
                     PLLEDVYVACEVLKCFGSRVNIWNNTITIDNRGIYLGEAPYELIKKMRASFLIMGPLL
                     ARYGRVRISLPGGCAIGSRPIDLHLKGFSALGAEITLGFGYIEAKADGLIGNKIYLDF
                     PSVGATENIMMAATLAKGQTIIENAAEEPEIVDLSNFINTMGGNVRGAGTGTIRIEGV
                     ESLHGTAHSVIPDRIEAGTFMVAAAMTRGNVLLENALIDHLKPIEAKLKEAGAIILEE
                     GEGIRVKGPDTLNPIDIKTLPYPGFPTDMQAQMMALVALAPGTSIITETVFENRFMHV
                     SELKRLGARIKIEGRSAIVEGVKRLSGTKVRATDLRAGAALVLAGLLAKEETEISEIY
                     HIDRGYYRIEEKLAKLGAPIHRIP"
     gene            complement(25273..26022)
                     /locus_tag="VFD89_08840"
     CDS             complement(25273..26022)
                     /locus_tag="VFD89_08840"
                     /inference="COORDINATES: protein motif:HMM:NF020264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HZJ58321.1"
                     /translation="MRYRIWIIGMVIAVLCTVLYTDLTRERLNADSSVLGSRESVIVD
                     ELLEASGCRWTKTDISAWAVLGFEIPSGETLKEMSIEAGEALNLDMEAEPILEKYGET
                     MEINLQGNGKNGLKYDIIIVGGKNAHMIINVLSNDRFVDSQIVTNQLDEYFHSINTIP
                     RITTTFVGDLQGELNAGQRQTLVRSLIRGLKGSAIQSMVDDRLISIAGYSPMVETSPM
                     DGINFQIASRYNEYMDKTYLWIGTPVITTEY"
BASE COUNT         6086 a         7043 c         5508 g         7494 t
ORIGIN      
        1 agggttatta atagctgagc gccgtagata tattcccatt cttccatcct atacagactc
       61 tttaatatgg gctctgccaa tacccccagc cccacagcgg cgggaactcc aaccaatagg
      121 gtcaagcgga taccgagggt ggtcttgctt ctgactcctt ccatatcctt taaggcatag
      181 gattcggaga tggcgggaac taaactcata gccagtgcta tggtcaaaac cgcggggaaa
      241 tttataaggg tattcacccc accggtgagt attccataca gactggtagc tatcatcttt
      301 atgtattcta catatgtgtc caccatgggc aggggtatat tggccaaaga ggggttggcg
      361 gggatttcga tccccttttt cgcggcaaat tctataaagg actccagacc atagggtata
      421 ttggcgatct tatccactat gggtgccagg cggtttacca ctatgacctg atcgctcatg
      481 ttcaccaggg gcattataga cgcccctatg gttacgggga ctgctatctc tatgagcctt
      541 aatattatat ccttagaggg cgcttgatag tgggacctgt atgtaaggcg ggcgttgtgg
      601 agtatcgccc ttctctccct gcgaaatatc ccaagaagga gtaccagggc cgctacctca
      661 ctcaaggtta cgccgacgat ggcccctgcg gcggcaaatt ccggtcccct gggaagccac
      721 agataagcta aggtcagacc cattataagc ttgcccagct gctccactat ctgggaaaga
      781 gcggtaggat gcataacgtg cataccttga aagtatcccc tataagcaga cattatggaa
      841 acaaagaata aggctgggga aatggccata agtgcataga tggatttggg gttaccttgg
      901 attttagata ttagtccgct gccgatgagc agtatgacgg atgtggcgat tcccagaagg
      961 gttaggagcc ttcgggagat cctgaagatc ctatagcccc catagtggtt gccggtagcc
     1021 agctcctcgg aaaccatctt ggatatggcc acgggcagac ccgccgtgga tgccaccaat
     1081 aaaaatgcat atatcggata ggccatctga tagaggccta tcccttccgg gccaattata
     1141 ttataaaggg ggattctaaa cactgccccg attatcttga ccaccaggcc cgaaactgcc
     1201 agaatccccg caccttttac aaaggatttt ttgtccatac cgaacctccg ttcactacaa
     1261 agcataatga tattatatca ctaaatccag ttatgaacag gccttttcat gtgatttgga
     1321 ggctgtccgg aataaaccat tttccctacc aaaaatatcg ggttttagag agtctgggcg
     1381 gtaataaacc ccgccctact tccagcccct ggggtttttc ttaatggatt cattaaagtc
     1441 gtccaaatgc tccctgagct ccttatactt ttcgggatac agcgccttaa catcatagga
     1501 ttcgtccctg tcatggtcta agtcgaacag gaaggaatgg atcatcatat tcttataggt
     1561 agcgttctcg ctgtttgtag ccttgtagta tttaaagtta tcctgagacc ttacaccaaa
     1621 cccctggtcc tcatattcgc cgggatcgac atagatgaga cagtcatggg ggctttcttt
     1681 agtctcaccc ttcagtaggg ataggatgct ctttccgtcg ataatcctgt ccttgggaag
     1741 ctcgaccccc gccatctcgc tgaaggtagg caaaaagtcg atattcatgg cctgctctct
     1801 tatggtaccg ttgacaacct tgtccttcca atagcatatg gtggggacca cctggccgcc
     1861 atcgaaggca ttgccctttc tgcccctatg cccaccgggt gagccttgat gccagggccc
     1921 gttgtccgat gaaaaggcta taaaagtatt gtcaaggagt cccttttcct ttaggagatc
     1981 aaagagctgg cctataccat ggtcaaattc ctctatacaa tcgccatata ccccgccttt
     2041 ggatgtaccc ttgaattttt caccgctatt taaggggtgg tgaggccatg gggaagcgta
     2101 ataggcaaag aaggggccgt cgctattatc ttcaataaat ttaaagaact ccgtatttaa
     2161 gtattccgtt atcttgctct ggtcaaaggg ggcctccacc gctatctctt cgtttctcca
     2221 gaagtgatat gggaccatat cgttggaata atgcgccccg tagaagtaat caaagccctt
     2281 gttgttgggc aggtggggtt ctttgtcccc tagatgccat tttccgaaca tcccggttct
     2341 gtagcccctg gcctgtaata tttccgctac ggtcacctca tcgggcataa tcccctctac
     2401 agggatggct ccgccgatga atttggacgc ctgcctggcc ctctctacgg cttcccggct
     2461 gggccttcct ccggccagct cttcgggggt ttcatagccc ctgtctacca ccatatgtct
     2521 ctcttcaatc tccaccgtgg ggaagaatac attctttata aatcccctag agggatatct
     2581 ccccgtcata aggccgaacc tgctggggga gcatacggga ctggatgaat agcaattctc
     2641 cattatgagc ccttcctctg ccatcctatc caggtttggg gtatgtatag ctttggaccc
     2701 aaagcaggac agatcacccc agccaaggtc atccatcaat ataaagatta tgttgggtac
     2761 gtcctttttc tcctccgtag ttaatgggct tatggaggac aggtactctg cgtgggcatc
     2821 ttggcgtatg ctatccttgg ccctcttttt tctctcccct acctcttcca ttaatttttc
     2881 aaacttctca ttgacatttt ctttattggg catatcctca cctttttcag tattgttttt
     2941 taaatacaaa aggaaccata agcaaagggg aattaaaggg gacagccccc tatgctccat
     3001 cggtgaagca agaaaaaccg tccccttccc tttatctacg cctctagagc gctatcttta
     3061 ctgtactgct ttcacggttg gcggttatga gaagtccttc atcggtctcc ctcatggttc
     3121 cgccctctac gctggcaaca gagtcgatgt ttctaaggag tacgttccag ggcttttcgg
     3181 ggttatcaac ttctacccgg atttcgtcgc cttctctgta agcggcagca ttgagtccgg
     3241 cgcttccgtc caaatcggga accattaccc taataatatc tccgtcctcc atctcgaata
     3301 ggtggaagga gatatcccgg ctatagtcat aatcgggcct ttcgtcgttg gatcctactg
     3361 ggataacaga acccggtctt acccacaggg gaaggctcat atagccgtaa tcctcttcga
     3421 tccactggcc gccttccata accctgccgt caataaagtg ggtccaatta cccctaggca
     3481 gataatagct ggcctttcct tcctcgctaa agacgggggc caccagcaga gaatctccca
     3541 gcatatactg tagatccaaa taatcgcagg tggggtcctc cggaaactct aaatgcatgc
     3601 tcctcattac aggagtaccc ttttctaccg cctcacagga tgcagaatag aggtagggca
     3661 tcagggtgga ttttaatttg gtaaagtacc tcagtacatc caccgcctcc tcatcaaaga
     3721 gccagggcac cctatagctg gtgcttccat ggagcctgct atgggatgac agtaggccaa
     3781 aggcgcacca tctcttgtat acatcggcgg tggcggtatg ctcaaaccca ccgatatcgt
     3841 ggctccagaa cccaaagccg gacattccga gggatagtcc gccccttagg gattcggcca
     3901 tggactcgta gttggcgctg caatccccgc cccaatgaac cgggaactgt tgtccaccag
     3961 ccgtggccga gcgggcaaac agacaggctt ctccctctcc caatttttct tccagcactt
     4021 caaatacggc cttattatat atctgaggat agtagttgtg cattctctcg ggatccgaac
     4081 cgtcgtggta gactacatcc gtggggatcc tctcgccaaa gtccgtctta aagctatcga
     4141 cgcccatgtc tatgagcctt cttagctctg atttatacca ttcgtaggcc tccgggttgg
     4201 taaagtccac cacggccatg cccgcctgcc atagatccca ttgccataca tcgccgttgg
     4261 gtctctttaa caggtaaccc ttttccttcc ctatatcaaa gagcttggag cgctgggcta
     4321 tataggggtt aatccataga cagatcttaa ggcccctggc cttcagcctt ttcagcatag
     4381 cctcggggtc cgggaacacc cgctcgtccc actcaaagtt acaccaatga aactccttca
     4441 tccagaagca gtcaaagtgg aagacgtgca gggggatatc cctctcggcc attccgtcaa
     4501 tgaagctgtt taccgtatcc tcatcgtagc tggtggtaaa ggaggtggtc agccacagac
     4561 cataggacca tgccggaggc agtgccggtc ttcccgtcaa ttgggtatac ttatccaaaa
     4621 tggactttgg ctcgggacca tatattagaa aatattctat gtattgtccc ggaacggaga
     4681 actgtaccct gctgaccttc tccgagccta tctcgaagga caccttctcg ggatggttga
     4741 cgaatacgcc gtatccttta ttggtcatat agaaggggac attcttgtat gcgatttcgc
     4801 tggaggttcc cccgtcttcg ttccagatgt ccacggtttg accgttcttt acaaaggcgg
     4861 taaacctttc cccaagtccg tatacattct ctctgacacc tagggacagc tgttcatgga
     4921 cgtaggtatc gccgtcatgg gtttcgatat agcccatggc cttatcctca ctcctggtga
     4981 tgaccttgcc atcgcctata aagtctacgc cccatcttcc ccttttattg atcctgacct
     5041 tgagtcttcc cgaggttaag gaggcgtagt cctcatcttc gctgatttct acccgggtat
     5101 catccctttc aaagatctca aattccgggg ttttagcggt gcccccctta aagttataga
     5161 acctgaccct tattacatcc tccatggggg atgaaaattt agcggtaagg agcttacaat
     5221 tgagggtatc gccccgatcc tccacgggcc ttggcggcgc aaaggcggta atggaatcct
     5281 cgtccatttc caaatcatgg aggtggaccg gaaagtaggc atccagatct tccctcatca
     5341 accaatatcc ctttgtaaac ttcatctaat caatcctttc attatgaaaa ataattatac
     5401 caaaacactt ccccactatt gggttttttt tgaatgaaaa tgtttttgga tctcttcttg
     5461 acggggcctg atataccggg ctccgagtga tggacttcca acttctaact tctaacttcc
     5521 aactttaatc atactatatt ttacccgtat taacaataat ctgtcgggag acatgactat
     5581 tacttatgtt acgaaaagga cctttcattg taaaattggc taaattatcc aattttgcgt
     5641 tagaatatgt attttccata acataggaat taaggacatg cttaaggatg cctgtgtacc
     5701 aatgtagagt gtttgtggca aaacatatag gcaccaaaaa ggcattgaac ccttcaatgc
     5761 cttctcagta ttattcgatt cgattttaaa ctagtgctcc atctgctttg ctaaaaaccc
     5821 ggcagccgtt tcggcgactt ttacttccga ttcacccata tcctcatccg tttccttgga
     5881 caaaagaatt atggtaccga tgggatctcc ttgggatatg atggggacta taacctggga
     5941 tgaatactta cccgcatcat caccattaga aacgatgctg aacaatgtat tattctggct
     6001 acgattggcc accatggtct ttttgccttc cataatcctt tcgatatcca tacttatgga
     6061 cttttcgtga aattctcttt tagagcctcc ggccactgcg ataataaaat ccttatccgt
     6121 tatacaggcg atatgcccaa gggtattatg caaagactcg gtatattcct gagcaaaatc
     6181 gcttagctcg cctatgggag agtatttttt aagtattact tcgccatcgc gatccgtaaa
     6241 gatttccagt gggtctcctt cacgtatacg caaggttcga cggatctcct tgggtataac
     6301 tactctgccc agatcatcta tccgcctgac aattccggtt gctttcacag acatccctcc
     6361 ttgatataca cattattaca aaatcatcct tgcaacctta ttatcttact atcacatcag
     6421 ttttatgcaa tactcttgaa aactttcctt ttaacggaat aagcgattgg gaggccgggg
     6481 tgttcacagc ctcttttcaa attttttcac cagcttttct ttccagtcgt ccactacttc
     6541 cttccacttc tcgtcctgtt cttgcctgtc caagatctcc tttatatcgt cccttttctc
     6601 ttccagggta taggtctttt ccgaatattt ctcttccaat ttaattatat aatagccgtt
     6661 tggcgtggcg acaaggtctg agaccccccc taccttgagt ttaaaggcgg cttccttgta
     6721 ttcctccgga agatagatgt tctccttgtg tatggttatg ccctctttca tctcttcggc
     6781 agaatcgggg ttgacttccc ctaccatttc atcaaagtct ccatccttct ttgccttatc
     6841 caaggccttt tgggccttat cctttatggc ctccagctct ttatccaaga gctccttggc
     6901 ctcgtcttcc tttttatcgt ttaggagcgt cctgtactca gtcacctttt ccccgggtat
     6961 ctccacaagg gcgtgcttaa cccttatctt gggagggtcg aaaaggtcca cgcttcggcc
     7021 ctctaaaaag gtcgtgttct ctttttgttt ggccaattcc tcgttgtaga attcctctat
     7081 atccgatgcc gaaacctcaa ttccctctac ggtcttatca tatagctttt ctatgcattt
     7141 ttgctctgct aaaaatgcta catactcctc ctgggtcatg cccatggttg ccaggtagtt
     7201 atcaagataa tcctcggcct ccttgacata gtccttttcc tgggactcct cgtcttcatt
     7261 ctcctcgtca gcctcttcca gctgcctctg tatgctcttt ttaatgtccg ccaggtcctc
     7321 cctagtctct tccataagct ggtccgttac tgtaaagccc gcggcggcgg ccttttggtc
     7381 caagagcttt tggattgcca actggtctag aatgctcttt ttgatctcca ttacatgttc
     7441 cctgttgttt ttatctctga ggccctcgtc ggtcaggtta tgggattcct tctccctgtc
     7501 caattggtcc aatacttcgc cctttagaat ctcctcgcca tccacctgag cgaccacctg
     7561 ggccctgtcc ttttcagggt taacctgtac catgccacag ccggccatga tccccaacat
     7621 tgatgcgatt acaagcaaga tgcttatctt tcttatgtgt tccatctctc ttcttccctt
     7681 cgtttccata gaattatacc ttaattatac catactatcc cagcttaacc atctgctcta
     7741 ggatgtcctt cgtctcggtt agagccgtca acggggcatc atcccggagg attatcctga
     7801 acatgggtcg tctcatcccc tcatacctga gcctgccccg gttttcattt aataggacca
     7861 taaggctcct ggaatcgaag gttttagatc ccgtcagcct taggataacc tccctgtccc
     7921 tatgggatac ttccgaaatc ccgagccctc ttgccaggcc cttaatgtag gctatatcta
     7981 taaggttggt cacggggctc ggcatatccc caaaccgatc ctccagctcc tcctctatat
     8041 cgtacttgtc ctgaagcccc tctatggcgg caatcttttt atatatttca atcttttgcc
     8101 cctctagggg aatgtaatcg tcgtctatat aggcgtttac ccttatatct atggttatat
     8161 ccaagggctt tggtaccgcc tcgcccttta ggctcttaat ctcctcatcc aacagcttac
     8221 aataaagctc gtagcccacg gcggtcatgt ggccgtgctg ctgtggcccc aagatattgc
     8281 cggaccccct gatctcaagg tcccgcatgg ctatcttaaa cccggcacca aattccgtaa
     8341 attcctttat ggcctggagc ctcttctctg ccacctccga taatacctta tcctttctgt
     8401 aggtgaggta ggcataggcc aggcgattgg acctgcccac cctgcccctc aattgataca
     8461 gctgggacag gccgtagtag tccgagtcgt atactattat ggtgttgacc ctgggtatat
     8521 ccagaccgct ttcgattatg gtggagcata gaagaagatc gaactttccg tcatagaagt
     8581 ccagcataac gttttccaag agggattcgc ccatctgccc atgggccatc ctgatcctga
     8641 cctggggaac caactcccta agcctttcgg ccatccgttc catgctcctc accctgttgt
     8701 acacaaagta tacctgcccg ccccgggaga cctcctttaa tatagcatcc ctgattagag
     8761 aatcgttgta ttccaccaca taggtctgta ccggaatcct gtcctccggc ggagtctcga
     8821 tggtgctgat atcccggatc ccaactaagg acatgtggag ggtgcggggt atgggagtgg
     8881 cagtaagggt cagtacatcc agattctttc tgatgttttt gatggtctcc ttgtgcccta
     8941 caccaaaacg ctgctcctca tcaaccacca gaaggcccaa atccttaaat accacgtcct
     9001 tgccaaggag cctgtgggtc ccgatgatga tgtcgatatt gccctgcttt agggcccgta
     9061 ggatatcccg ctgctgagca ggcgtcctaa aacggcttaa gacctggatc tttacgggga
     9121 aatccccaaa cctttccaca aaggtgttaa agtgctgctg ggccaagatg gtggtgggcg
     9181 ctaagagcgc cgcctgcttg ccgtccatta ccgccttaaa ggctgccctt atggctacct
     9241 ccgttttgcc gtagcccaca tccccgcata ggagcctatc cattaccctc ccgctctcca
     9301 tatccttctt tatatccgtt atagcccttt cctgatcggg ggtctcctcg taggggaaca
     9361 tctcttcaaa ctgattttgc cagggagtat ccagggaaaa ctggtgtccc ttggtggtct
     9421 ctctggcggc atacaattcc accaaatcca tcgccagctt ttggaccgat tggcgagcct
     9481 tggtcttggc cctttgccat tcggctccgc ccagcctgga tagtctgggc tctttttggt
     9541 ccatcccgat gtagggctgg atgatatcca cctgatccgt ggggatgtac agcctgtcgg
     9601 tgcccccgta tttaatattc aagtagtccc gcttctgatc gttaattact agggtctcta
     9661 tgccaagata cttccctatg ccatgggcct catggaccac aaaatcccca atctttaggt
     9721 ccgtaaaggg gtctaatttg cccttcccgc taggggcctt gctctttcgc ctatgcttca
     9781 tagttccgaa gatttcatta tggctgatca cggcaaattt gccctgggga tattcaaatc
     9841 caccgctgat attgcctgaa aggaccatga tctgtcccgg catgatatgc ccctctgcgc
     9901 ccgggctaaa ggtggcttct attcccagat catacaggtt tgagaccagg ctttgacacc
     9961 tggacccgct gccggcgaac agaataaccg aatacctctt accacgccag tacctgagat
    10021 cctccgccaa aatgtccagc ttgccctggt aagaggggat gcccctggat gcgacgttga
    10081 atatggccct gggggaataa ccgggtacgg atttaggtag cccctggagg gccagcctgt
    10141 gaaacttctc tgattgagcc ataagttcct gatagcttat gaatatctcg ccctgtcccg
    10201 ggagcacttc cccgtcctct aacagggttt taaagtgctc tataaactcc tcttcccatc
    10261 ccgaaaagct ttcacccatc cgggagggtt cgtccaagac cactaaggat tcgggactca
    10321 tataatcaaa gagggagtta ttctccgggt agaaaaagga gaaataattc tctaaattcc
    10381 tactctgcat cccctgctcc atatcctcta agagggcgga tacccttgtc ttgggcccct
    10441 gctttccctt ctttgttgct gcggccagat ccctttttat cccttcaagg cccctagcat
    10501 gggatttcgc cgtcagaacc agctctctag cgggagagat ggataccccg tctagctttt
    10561 ccgtggagcg ctgggatagg ggatcgaaga gccgtatgga atccacttca tcatcaaaaa
    10621 attctatcct acaggggtta tccatggtca tggggtatac atccactatg cctcccctga
    10681 tgctaaactg tcccgggccc tctactgcgg gcacccgctc atagcccgcc tctaccatct
    10741 tgtaggccag atcccttatg gaaaacacct gccccacgtc cacccttata agcccaccct
    10801 taaagatttc ggggggagtc tgaaggcata aaagggcctc tatggaagcc accacaaccc
    10861 ggtattcccc aagggccaga gcctctaaga ccttaagccg ctgacctgaa acctcggtgc
    10921 tgtgggccgc cacatcatag aggactatgt ccctcggcgg aaggagcatc acgtagtctt
    10981 ccatatagaa cctcaagtcg tcatagaccc ttttagcctg aatatggtta taggttatat
    11041 aaaggcattg cctgcttagc ggatatagga ggctggatac tgcatggttt ttttgggatt
    11101 ccgaaagtcc aaaaaccaaa atagggccgc ccccttccct ggcggcctgc cgcatcttta
    11161 taaactcatc ccacctcgtc atgggatcca gccattttac cgtcaaaaaa tcacctcaat
    11221 cttgaaagct aggaggcggt gtttttgcct ccctttcgct ttctagccgt tgcatttgct
    11281 catggcgtat tccacgccct ttgttattat aagctctacc ccttttaccg ccctttcgca
    11341 ggcctccttt aaagggggaa attcgtcatc tttaaattta gacagcacat agttcttaag
    11401 gtcccagccc tcgggcgttt cccctatgcc tatgcgcacc cggggaaaat cctggctctg
    11461 tagctgatat atgatagatt gcatgccctt gtgggttcct gcgcttcccg aggacctgat
    11521 cctaaggacc ccgacatcca gatctatatc gtcatatatt acgataatct tagaatcctc
    11581 caccttatac cagttcttaa tatctaaaat gctctccccg ctaaggttca tataggtctg
    11641 gggctttgcc agcacaaccc tatgaccttc gaaagtcccc tcccctataa gggccttgtg
    11701 tttaatcctt gctaccttga ttccaagtct ctctgccagc atatcaaggg cgataaagcc
    11761 tatgttatgc ctggtatcag catactttga tcccgggttt ccaagccccg ctataatata
    11821 cataaaggcc tcacctattc aaacaaggtg ctcaccggca gatcctcgta tattctgtcg
    11881 atggcctctg caaatatggg ggctacggac agcacatgga tcttgtcgat ctgcttttcc
    11941 ggtgtcaggg ggatggtatc ggtaatcacc atctccttta tgggggaatc ctgcaatctt
    12001 tcaatggctg gtccggaaag aacgccatgg gtagcacagg catatatctc cttggctccg
    12061 ccatctaaaa gggccagagc gctttgaacc agggtccctg ctgtgtccgt catatcgtct
    12121 actaacaaaa ccttcttgcc ctttatatct ccgattatgt tcatgacctc ggccacattg
    12181 gccctgggcc tgcgcttgtc tactatggcg atgggcgcat ttaaacggtt ggcaaagttt
    12241 ctggccctgg ttacgctgcc gatatccggc gaaaccacga tcaaatcctc gtgttcaaac
    12301 cccttctcca agtaatactg ggtcaatatg ggaacgccca acagatgatc taagggtatg
    12361 gagaaaaagc cctgtatctg ggcggcatgg aggtccatgg tcaaaacccg gtcggccccg
    12421 gcggtggtga taaggtccgc aatgagcttg gcggtaatgg gatctctggc cttggcctta
    12481 cgatcctgtc tcgcatagcc gtaatagggc ataactgccg ttatcctacc agcggatgcc
    12541 cttctgaagg catctatcat tatgagcatc tccatgagat tgtcgtttcc gggaaagcag
    12601 gtggactgga ctacgaatac gtcggccccc ctgacagtct catgaatatt tacgcttatt
    12661 tcaccgtcgc taaatgtgga tacctccgaa tttcccatgg gaatgccaac atgctccgca
    12721 atctccctcg ccagtttggg gttggaattc cccgagaaaa tcttaatact tgttccgtgg
    12781 gttatcattg gttttcctcc gtttcattct cttcactctt tacccaattc tctttattca
    12841 cttgttttgc ccgtgcgatg gccagggcgt ttttcggtac atcctgggtt atggtagacc
    12901 ctgcggcaat atatgaattg tcctccacct taaccggcgc taccaggttg gtgttgcagc
    12961 ctacaaaggc attgtcaccg acgacggtcc tgtgtttctt tttcccatcg tagttgacaa
    13021 acaccacccc gcaccctata ttgacgttct cccctatctc tccatctcct acgtaggtta
    13081 gatgtgagac cttactgccc cttcccacct ttgcgttctt gatctccaca aagtcgccga
    13141 tcctcacgtt ctcctctata aggcttccgg gcctcagata tgcaaagggg cccacagtgg
    13201 tcttgtcccc tacatggctt tccaaaatca ccgaattttc tatccgggta tccttcccta
    13261 taacagaatc taggattcga ttgttaggga gcaaaatact tccctcgcct atggcggtat
    13321 gaccctctaa gaaattgccg ggatagatta tagcatccct gcctatggtc acatggggtc
    13381 ctatataggt attatcggga tcaattatgg ttacgcccct ctccaggtgg cgactgttaa
    13441 tgcgctgccc cataattttt tgtgcctcca caagatgaac cctggtatct actgccatgg
    13501 tctcggttga atcgtatacg gtatgaagcc ctattcttcc ccccgaggcc tttaagtcgt
    13561 tcaataggcc gataatatgt cgatggtccg tatccttgcc gccaaacctg tcaaggccat
    13621 ccaaaagggc ggaaaccctg aagcagtaga cggatgtatc gatctcctta atcaggtttt
    13681 cttcgtctag ggccttcaca tcctctaaga tcccctcaaa gtccccggaa atattcctta
    13741 aaatcctgct gtatccctgg ggtttatcgg atagtgcgga cagaaccacc gcatcatatt
    13801 tgcccccttg acaactgtcc gccagatcct ttagggtatc aggcgttaaa aggggcagat
    13861 tgccgtccat caatatgaca tagccgacat cgtcctttaa gtaatcctta atcagtgggg
    13921 ccccatgacc catctgtgcc gattctggca gatgagaata gttcacccgg gtccctagcc
    13981 gctcccctat gccctccgat ccttccccta agatcactaa cgggcgaata tttactacat
    14041 caagcactga ggcaaccacg aattctacca cgggctcccc gcagattttg tgcatggctt
    14101 taggcaggga agacttcata ccgggtccat accctgccgc cataataatc gctgatattt
    14161 tattctccat tcctttcctc cctgttccac ctttcgatgc cataccaaaa agatacgatt
    14221 ggtgccaatc ccaccgcata atccacgtgg tacccaaaaa caaggctatg aaaatataat
    14281 ggccaattag gatcaggtgc ggtcacggat cgctattcca ttatacatga aatctgtgat
    14341 aaagtttacc tcatacatag aaataaaaca aatattttcc caaaaattta aataagcttc
    14401 aaaggagatt gctctccctc gaagcttatt cctcgtcaat ctgatcatat tgttgtaaaa
    14461 tcattccttg gagccttgcc ctcatatcag aattgattgg atgggcaatg tcccgatact
    14521 ccccatccgg cgtctttcgg ctaggcattg caataaacat tccgctttga ccctctatga
    14581 tcttgatgtc atggactaca aagtcattgt caaaggtcac ggaagcaacc gccttcatct
    14641 tgccatcacc accgactttc cgaaccctta catctgttat ttccaattct agctcaccac
    14701 cttccgagtc gcctaacaag taagatacag ttatatacct attcgccaaa aaaatccata
    14761 gtcctgcctg tatcggaaaa atagtgaatt gtttttgtat aattaatttt ttgaaacttc
    14821 ttttaaaggg cattaatgca tttaggaagc cccctttaaa aggtgttaat ccgttttgaa
    14881 actaccttta aagggcatta atccatggtg agggctccac tacgaccttt cgctccccct
    14941 cgtccacctg ttttaatacc atgagagact tataattttt gaccattttc tggcccgggt
    15001 cctcggtaga gattatcacc ccgaccccgg cgatctccgc ctcaaattcg tggaggagtt
    15061 ccataagtcc tttggcagtt cctccgccct tcataaagtc gtctactata agccctcgct
    15121 ggcccttctt caccgccctt ttaaccaggg acatggtctg aatgcgcctg gaggatgccg
    15181 aaacatagtt aatggatatg gtagaccctt ccgtcacctt gttttccctt ctggccatga
    15241 ccacaggaca attgagggcc ctggctgcgg ccagtgctat gggtataccc ttggtctcga
    15301 cggttatgac aaagtccgga cgatcgttat agaattgggt tgccaaaata ttgccgaatc
    15361 gttgcaccac ccccggcata aacaggatgt cagtcatata caaaaatcct cccggaagta
    15421 tcctgtcggg taccgagagt gtcgtgcaga tatcctttat aaagatttca tcgtctttaa
    15481 acagccggga tacgaacctg accccgcccg cggccccgac tacggtttca acatcgccca
    15541 gctgaaatct gcccaggagc tcccttatga ttaccacatc gtcgcttatg gtagatttcg
    15601 ctgttttaaa catatcgcta aaatggttga gggtaaatat cttattggga tgattgcata
    15661 ggatattgac cattgcgcct atccgttcgt ttctcctcac cttttccatc caatcactcc
    15721 ttaacaataa caaaaaaacc taagcctgcg tgggatgatg gctgtacgtt atccccgccc
    15781 atgccctata attagctgca gccaatgggg gcaattgtac gttatccccg tccaaaggct
    15841 ataattaatt atagcacagt ttcctggata tatccgaatg tttttttgat tttctggatt
    15901 tttattcggg ttgggtcctg cccccgcccc tttcccttcc ctcaattctg ttatataata
    15961 accaagcagt gcggggactc caaaacatat gataattaac gggatttagc ttttctgagg
    16021 gggataagac gaatgagaaa gatactcggg tgccttaaaa gggcggtaga agattttaat
    16081 atgatagagg agggagacag gatagccgtt ggggtctcag gcggaaagga cagtctggtc
    16141 cttttaaagg ccctgaggct ctaccagtac atgtctcccg tcgaatacga gctggaagcc
    16201 attaccttgg atatgggcta tgacggcacc gactggaccg gggttaaaga tttttgtggg
    16261 gatcttggca taccctatac cattaaggac accaatatag ccgctatcgt ctttgatgtc
    16321 cgccgggaaa agaatccatg ctccctctgc gccaacttaa ggcggggggc cctcaacaac
    16381 gcggccctgg ggcttgggtg caaaaaggtg gccctaggcc accaccgcga ggatgttata
    16441 gagacattgt ttctcagcat gttctacgag gcgcgcctta acaccttctc gccggtaacg
    16501 tatctggacc gaagggatat taccctcata aggcccatga tatatgtgcc ggaaaccgat
    16561 attaaaaggg ccgtatcccg ccatgatatc cccgtggtat tcaatccctg ccccgcaaac
    16621 ggcaacacaa agagggatta tataaaaaat ctgctccacg acatatccgg ggaaatcccc
    16681 aatgtccatg agcagatatt aggtgctctt agaaaaacta aggtgcgcaa tctgtggaat
    16741 gattaaaatt cccttaggtt gcatccccaa ggccaaacat gatgctaccc tccgccgcta
    16801 cctggccttc aaccaaggcc ttcacgttgc ccttgcctat ggatcccctc atcctggtta
    16861 tctccacttc catggcaagc tggtctcccg gtatcacctg tcgcctgaag cgcatggaat
    16921 ctatcccggc aaaaaaggct aacttgcccc tgttttccgg cagacttagg accaccatcg
    16981 cccctaattg ggccatggcc tccacaataa ggaccccagg cattatgggc gcacccggaa
    17041 aatgtcccgc aaaaaacggc tcgtttatgg tgacgttctt tatgcccttc ccactcttgc
    17101 caggtaccaa ttccagcacc ctatccacca acaggaaggg atagcgatgg ggaatccgct
    17161 ccattatctc atttatgtcg catacatcca attatatcta tcctctcaaa ctgttagcct
    17221 gcttttccat ctcatcggcg gtctgtacca ccctagaatt taactgatag gccctttggg
    17281 ctattataag gtcggtcatc tcccgggcca tatccacatt ggattgttct aaatacccct
    17341 gaaagacctt ggtctccatg gatcctacag gttctccgct atcgtcattg gccatgaaat
    17401 aggatccgtt tatacgctcc agaccgttgg gatccgaaaa accatagatc cccagcctgc
    17461 ccccataggt agcattacca tcttgatcat tcaaatatac gtcgccatac tcggtaatgg
    17521 agatatcctc ctcccgggtc ccgctgtcaa atacaaaaaa ttggccgtat tgatccagga
    17581 ccgggtatcc gtctccgtta tccagcctgg ttccgccgcc gggcagatgg gatagtctga
    17641 ggcttccgtc cctggtatag agatattccc cgtcgtcgga ttccacagta agaaatccgg
    17701 gaccgtctaa ggcaaggtcc agacttacat tggtgggatg cagggcgcct tgggtaaaat
    17761 gggtttgaac agagcctacc aaggttccgt ttcccaccga gggatttggt cccgcctctt
    17821 cgggtctgtt ctctataata ttgtagaggg cctccttaaa atccggcctt gatctcttat
    17881 agccgacagt ttgggaattg gctatattat tacctataga atccacctta tgttgttgta
    17941 ccctcattcc ggatgttccg gtatacaaag ctctgatcat atatatcccc ctataagctt
    18001 cccacttcat tgacggtctt ggaaagggtg ctgtcgatca tctgaagaac cctttgattg
    18061 gcttcatagg ctctggatgt tattatcatg ttgctcatct cctctaggag ctctacgtta
    18121 gatgcctctg catatccctg aaagacttgc ccctcaaagg ccagggcttg gtttgctccc
    18181 gggttgttat tgacaaacag attatccccc accttgtcca gcatggtggc atcttcaaaa
    18241 tctaccaagc gaaaactgtt tacaaatccg tcatcttgcc atatattgcc ctcatcatct
    18301 atatcaaagt tcccttgacc tacatggatg ggaccgtatt ctccgtccac ggtatgccct
    18361 tccatggtga ctaaaaagcc ttcatcgtct actttaaagg ttccgtcgcg ggtatagaat
    18421 tcttcgccgt ctctcagtat tgtaaagaac ccgttgccct gtagggctat atcggtaatg
    18481 agatccgtct gctccaaggc tccatcctca aaaagcggta ccgttctttc cacatatacc
    18541 ccatggttta gcggacccag gggttttacc ccgccggctt gtccgcctgt tcgtagcaat
    18601 aactcgtccc tgaaggaatg gctgaccacg ctgtccctct tatatcccct agtattggta
    18661 ttggataaat tattggctat ggtatttagc ttgtcacgtt gatgtatcat ccccgtggcc
    18721 gcggtataca gacctctcag catctttacg catcctttca aaatcgaaaa tctatggtaa
    18781 tcaaaatcta tggttcccct cgcccataaa cagcctattg tccctgtcag tcggctggat
    18841 agccaataga ggcaaggtat taatcgctaa tagtcatgcc ttgttaatta aattcccata
    18901 atggcaaccg aaccccatga cacaaggaat cttcactaca aatatggtgt catacaccga
    18961 tattagtatg taagggtccc gcccctgtaa ttaaatgcag gggtgtcggt atacagatca
    19021 atatcctcaa gtgccttgcc cgtccctatg gcaacgcagg atagggcatc ctctacaacg
    19081 tttatcccga tcttaagcct gtcctctagg agcttgtcca gcccatgaag caaagatccg
    19141 ccgccggtca ttgtaatgcc gatttcgctg atgtcagagg aaagttccgg aggcgttttc
    19201 tctaaaacgg agtgtatggt ctccgctatg gtttctaagg gctcttccag ggcctccacc
    19261 atctccctag aatttacgct gatggtcttc ggaaggccgg agattaaatt tctgcccgtc
    19321 acatccatga ctacatcctc tttacggggg atggcagagc ctatatttat tttcatcttc
    19381 tctgcggtcc tgtcacctat taaaaggtta tagtttttgc gcatatatct gactatggaa
    19441 tcatcaaaca tatcaccggc gatctttatc gaatcggtaa cgacggcgcc gcctaaggat
    19501 ataaccgcga tatccgttgt tcccccaccg atatcaacca ccatatttcc atagggtttg
    19561 ctaatatcta taccggcccc gatggctgcg gctatggcct cctctataag gtaggtgcgt
    19621 ttggctccgg cctcgtagga ggcatctata accgccctct tttccacttc ggttacgccg
    19681 ctgggcacac aaacgattac ccggggtttg cgccctatgc gccgtacaat ggcccttcta
    19741 aaaaagtatc tgagcatctt ttccgtaatc tcatagtccg atatgacccc gtcctgtagt
    19801 gggcgaaggg ccacgatgtt tcccggggtt ctccccagca tcttacttgc tgatgatcca
    19861 acggccagga ttttcccacc ctttaaatcg atggccacca ccgacggttc tctgagtact
    19921 acgcccttgc ctcgcatgta tatcaaaacg cttgatgttc ctaaatctat gccgatatcc
    19981 ctattagcaa acactatcta tcggtccctt ctcttgcttt ttatgtatat ctgttaactc
    20041 ttagttgatc agggcatttt ataaatgttt tacgatcctt caaacgcgat ataaggcctg
    20101 tatcgcaaaa catataagct gtcatagaat aaataactgg tcagtcgaag tttcaaaaag
    20161 ttgagtaatc gacattatta tcttaagtat catatagtgc attttttgaa acttctcaag
    20221 ttaatgcagt agaaaacata ttggtaccta aaaattaccc ccaataaata tttattctac
    20281 attttactcc aaaatcctct tttactgata aattttatcc cagatccgtt acaatctatg
    20341 tttactcggt gttttcttca tgggaagtat cttcattctc caattggtat ttaatcttag
    20401 ttgctctgcc gccccggata tgtcgctcgg ctttattttg atccagaact tttttgaccc
    20461 ctgcccaaag ggtttgattt accttgggaa gcctttcaga tacatcctta tggacggtgc
    20521 ttttgctaac cttaaagacc ttggccgttt cccttacggt ggctcctgtt tcgatgatgt
    20581 aacacgcgat ctcaatgacc ctttcctcaa tataatcctt cacaactaac ccccctcaaa
    20641 acaccctcat aacaaactgt aatccacccc atgctaaaat tacattcact ttccgtgttc
    20701 atagcacttg gccggctaca gtgggtatat tgataaatat gcgggttttg gcagatatag
    20761 aaggattttt taatataaaa gatcgccgat tttagagggt gcatcttggg ggttcacaac
    20821 ggtaaagtca actgtttgca tccgtgttgc ctcattcatc ccaaatgcag ccatagtttt
    20881 ccttaatagt tcgagcaatc tcctccgaat gacttatttc atagatcttt tgaagcgcgt
    20941 ccacacccca atttttttca tcgaatgttt tcgaatacat aaccacacca ttttgttaga
    21001 taatctatga attaataccc ggttttagtc tgttaaacgt cctccatagg ccggttatta
    21061 cagaagtcta aagccttcac attcttttgt tttcgtaata aaaaaaccac cgatcttatt
    21121 cggtggctat atatcgcagg gcaaaaaatc cgttttaaaa ccttaaagtt cccgggtgca
    21181 aatattgact gatctgtata atggccttcg tccctattac atatgtgtca ctaaccccgt
    21241 ccctatgaca cactatggca cggtgctatt ggagataggc caggggatcc aggcttttgc
    21301 cgtccagcat gacttcaaaa tgaaggtgcg gatcatccag tatctcgcag ctggccgttc
    21361 tgcctactcc gctgattatc tgtcccttgt ccaccttttg tcccaccgag accatattgc
    21421 cggtggatag gtttgcataa taggtcttca gcccattgcc atggtctaag gtgatacaga
    21481 ttcccatcaa gcgatcctct gttatctcct ctacggtacc gcccatggcc gccttaacct
    21541 ctgagcctac gaaggattct atatccattc cggaatgggt tgtccattcc ttcagggtat
    21601 ttgaaaatac aagctcgtcc atgatatgtc ctttgcttac attaccctgc acgggatata
    21661 tcatctgggc cttttgggtt gactttttag gggtttcctt cttttctttt ggggcctctt
    21721 ccttttttac cccttcgttt tttgtctcgc ccttgccctt tgcctcatca ttgtctttta
    21781 ctccgtcctt attctttgtc tcgtcttcct tcttatcccc tttgggggca agagtctcct
    21841 ttgattcccc ttctaatgta ccttcctcgc ttttatccac ttccggcggc ggagaactat
    21901 cctttatctt tatacttatc ttatcctcca gtatccccgc agtgtccagc ctatctacct
    21961 ttgtgggttc gggtagcttt attacatctt cgttatcggc aagttcctgg attggaggtt
    22021 tcgggttctt acccgaagtt aaaaaagcag taaccccgat tatagctata caaacaaaca
    22081 ggactatata aaacccctgc ttgtctataa agcgtattac cttttccttg gtaaaggctt
    22141 gcctaaacct gccccttgac ttatcggact tcatatttat gatacctcct tggtatttat
    22201 tgagttttca gtgttttagg aatcaattac ggatattcta ttcataattc tctccacttt
    22261 agcaaaaaaa atacaggtta tccaaggaaa atatccaatt tttccataga taacctgtat
    22321 ttttctattt actatatgcc tatgcgctat atacgatgga cacccactgt atttttatat
    22381 aaaaatgcct aaacaggggg cgttcttcat aatgggggac ttcttactat tgactaatta
    22441 acctttcacg gagcccttta tggatgtctt gcccatcaga tggtttcgat cttaataccc
    22501 ctataatagt gtcccaggat atcactgtag ttgtaaccct gccgggccat ggcgtttgcc
    22561 cctatttggc tcattcccac cccatgacca taacccgtcg tctcgatatg cactttatcc
    22621 ccttcaaacc tgaagtcaaa atccgtggag ttaagaccgt acaatgtcct aaactcggta
    22681 gccttaacct tagtatcccc aaccttcaga tcccttaccc tgccgctgtc ggtgcggctc
    22741 acgatcttta tcttctgttc caggttggat gaggacaggc cgctcttggt gtacttagag
    22801 ttaaatatcc tgacaaattc tttatttgtg agggatacgg acccccgata cctgggggca
    22861 tcctcttcac cggggctgtc taccacttgg tagtacggga gggacgcaga gaaaacctcg
    22921 gccacgtcct cagttttccc gttactggtc gaatagaaaa atacctcgat gggctttgcc
    22981 atataggtca tgatgtatcc ccttgtctcc tctatggcgc gcctgatctt tagaatattc
    23041 tcctcatagt tatcgcccca gttcttcctc tgttgggact cgggtatcca tgcctggcaa
    23101 tggataaatt cgctgcagac atctgcgccc ttgtgtttac tgcaaccgct gcccccgtaa
    23161 gagcgaagct ttcgggccgc tagggttctg gcggcaactg cctgagcctt tagagcctct
    23221 atctcgaagg atgagggcat ctctgctgct actaccccta caacatattc ttccaggggc
    23281 atatcctcca gagccttggt gtagtggtta tacaaggtta tggtaggttc ttggtaattc
    23341 acattgttac tttccacgcg tatgctgtcg ccactgctgc ggacctgaaa atcaggcgga
    23401 ggttcatcgg ggtctgtggt agagaacaat cccaccacca tcgatggcag gataagcaca
    23461 atgacaataa taaacaacaa tatatcaatc caatgtctca tagggcctcg cctttcgtat
    23521 tcaaattact acatttttgc gatatactat ttaggatatg atatatttat tgcatttaga
    23581 acattaattt tgtggcccag aatcaaaaag ggacgatcaa gatcgccctt gtatctcatg
    23641 tttcaaaacg gagcaaagat aacttttccc tctgctttgc cgattcccac tatcaaacac
    23701 acagtgaact ctcaccgcca aattacgcgc catgtacggc gcaccgcaaa agagaagtaa
    23761 aggggaccgc ccctatgttt tcctttatta cggatttcaa gggatgcgat ggatcggtgc
    23821 tcccagtttg gcaagctttt cctcgatcct gtaatagccc ctgtctatat gatatatctc
    23881 ggatatttcc gtctcttcct tggcaaggag tccggccagg accaacgccg cacccgcccg
    23941 aaggtccgtt gccctgacct tggtgccgga caaccttttt acgccttcta ctatggcgct
    24001 tctgccctcg attttaattc tggcacccaa cctcttaagc tcgctgacgt gcataaacct
    24061 gttttcaaat acggtctcgg ttattatgct ggtcccggga gccaaggcca ccaacgccat
    24121 catctgggcc tgcatatccg taggaaagcc gggatagggt aaggtcttta tatctatggg
    24181 atttagggta tccggccctt tcacccttat cccttcacct tcttctagga tgatggctcc
    24241 cgcttccttc agcttggcct cgatgggctt taagtgatct atcaatgcat tttccagcag
    24301 tacatttccc cgggtcatgg ctgcggctac catgaaggtg cccgcctcta ttctgtcggg
    24361 gattaccgaa tgggctgtgc cgtggaggga ttctaccccc tctattctga tggtaccggt
    24421 cccagccccc ctgacgtttc cgcccatggt atttataaag ttggataagt ccacgatttc
    24481 cggctcctca gcggcatttt cgatgatggt ctgccctttg gccagggttg ccgccatcat
    24541 gatgttctcc gttgccccta cgcttggaaa atccaaatag atcttgttgc ctatgagccc
    24601 gtcggccttg gcctctatat agccaaaccc aagggttatc tcggccccca atgcagagaa
    24661 gccctttaaa tgaagatcga tgggcctgct gcctatggcg cacccacccg ggagggatat
    24721 ccttacccgc ccgtagcggg ccaataatgg tcccattata agaaaagaag ccctcatctt
    24781 ctttataagc tcgtaggggg cttcgcccag atatattcct ctattatcaa tagtaatggt
    24841 attattccag atattaaccc ttgaaccaaa acacttcagt acctcgcagg ctacatatac
    24901 atcctccagc agggggactt cttccagggt gcatatatca tccgccagca acgaggcggc
    24961 cataatcggg agcaccgagt tctttgcgcc atggatcctt actttgcccg taaggggcgg
    25021 gctttgtttt actattaatt ttgccacagt cttcctccca agataaaatg atttgcgata
    25081 aacatcctat attatatctc tgctatcgat aaacacttgc cttgatttag cctgttacga
    25141 cggttattgg tgaacgaccc gatcccaact acaaaattat cacaagagat tgcccgggct
    25201 cattccaatt atcatcccga caagcttggt ttctctatta tctatatata gttcccgatt
    25261 ttttaaaaca aatcagtatt ccgtagttat aacgggggtt cctatccaaa gataggtctt
    25321 gtccatatat tcgttgtatc tggatgcgat ttgaaaattt atgccgtcca taggcgatgt
    25381 ttcaaccatt ggggaatagc cggctatgct tatgagccta tcatctacca tggattgaat
    25441 ggcgctgcct ttaagtcccc ggattaggct cctcaccaag gtctgcctct gccctgcatt
    25501 taattctccc tgaaggtctc ctacgaatgt ggtggttata cgtgggatcg tgtttatact
    25561 gtgaaaatac tcgtccaatt gattcgtgac gatttggcta tccacaaaac ggtcgttgct
    25621 cagtacattt ataatcatat gggcattttt accgcctact atgattatat catattttaa
    25681 tccgttttta ccatttccct gtaaattaat ttccatggtc tccccatact tttccaggat
    25741 gggttccgct tccatatcca aattcagggc ttcgccggct tctatgctca tctcctttaa
    25801 tgtctccccc gatggtatct caaaacccaa gacggcccag gcgcttatgt ccgtcttggt
    25861 ccatcgacat cccgatgcct ccaggagttc atccacgatt accgattccc ggctacccag
    25921 gacagaagaa tcggcattta gtcgttcccg ggttagatct gtatagagca cagtgcacag
    25981 gactgcaata accattccaa taatccaaat cctatacctc aaagctatca cctgctaaag
    26041 agtatttggg cttgcttcta tccgttatgc cggtaaatat ggtgtgcggc agacagcatc
    26101 cgccatatta accgggccca atggatattc t
//
LOCUS       DASRNX010000224        11631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_96248,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000224 DASRNX010000000
VERSION     DASRNX010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96248"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..1412
                     /locus_tag="VFD89_08845"
     CDS             339..1412
                     /locus_tag="VFD89_08845"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HZJ58322.1"
                     /translation="MDMPMKVIPGYFNSKPGIQVGTKMQGPITRDEEGNIKLDKDGVA
                     FLQQMGIEWVMLGHRQVPEHTVQCYKELKDALSEVGLKIFRLENYELHNMPDITLGLD
                     NRDEMLDRYLQYISDLGEAGIHYATYAHMGNGIWRNYEGGTVRGGALSGGLDLNAPNR
                     SIAFGDYEWPLSHGRTYSEDELWENYEYFIQRVVPVAENSEVYIGIHPDDPPACTIAG
                     VPRCIFSSLNGYKRALEMADSPNIGVCLCVGCWLQGGDEMGSTPEEFIKYLGENKKLF
                     KLHLRNVTAPLFSPGGFNETFPDAGYYNLVNIIEALDEIGFDGIVINDHLIDMVGGHY
                     TCEAYFTSYLKGAVDAIQNHRRK"
     gene            complement(1542..2492)
                     /gene="pfkB"
                     /locus_tag="VFD89_08850"
     CDS             complement(1542..2492)
                     /gene="pfkB"
                     /locus_tag="VFD89_08850"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HZJ58323.1"
                     /translation="MMILTVTLNPAIDKVYEVESFAIGEVFRPSGMWATAGGKGLNVS
                     RVAGILGAEVTAMGFLGGGNGQFIRSRLHEQGIRDAFIEIEGDSRICIAINDPISDTS
                     TEVLEWGPVISPKEQEGFLIDFKNIAKKADIVTISGSLPRGVAVNFYGRLIEISKGLG
                     KRLLLDTSGQTLVKSLEYRPFMIKPNETELETIMGKKMDSEYEILEASKEIHQNGIEL
                     VCVTLGAGGCIAATDEGAFILKAPPIETISTVGSGDSFIAGCAVALTQGKAIEDVLRT
                     GMACGMANTQFAGTGHVSRELVNKFFNLVTVSRGRGQRHI"
     gene            complement(2778..4457)
                     /locus_tag="VFD89_08855"
     CDS             complement(2778..4457)
                     /locus_tag="VFD89_08855"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZJ58324.1"
                     /translation="MVDPNIVETYTIGKEGFIVALCESLGVPEIINKALGSVNGRPPD
                     IPYGVDAMMMMVNMCDDHMPLSRLHEYYQYKDLEGLFHHPIKLEQINDDRFGGFLDLF
                     HGAGCKKIFSQIAGKAITLYGIQVTNINFDTTSKVMWGLYETYEGTEGVIKMDFGHSK
                     DRREDKKQIKFGIGCANGLVVDATVLSGNKDDKTYNTDTISALDATLHNLNVDKGNFY
                     YIADSALFSKENLDLSTEKGIKLITRIPDNVLIAKESMEEAVDILDTLENIEIINAKG
                     EPKPYSLLEKTCIYHEHELRLTVCYSPSLKASKEKTADKAIKKEMERITKVSKVLTKR
                     EFACEDDANREIQKINSKEFKKLKYHDINFSLRREERRKRGRPAKGEDNKILGYRYFI
                     ELSPVLNQEKTTKAINKACCFVLCSNDLGMTGEALLREYKTQDSVEKKFQQLKSPQFV
                     NSIFLESPERIEAFSYLMLICILMLSLAEYVVRRGLMQDDDYIIGPRKTKMNRPTQRV
                     IYDVFYAVMIRVICYPDRPWERSYARPLNESIKKILKYLKIPEDTFIKGSS"
     gene            complement(4687..5859)
                     /locus_tag="VFD89_08860"
     CDS             complement(4687..5859)
                     /locus_tag="VFD89_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HZJ58325.1"
                     /translation="MDIKSEALKLHGEWKGKIEVKNTVKVDNSRDLSLAYTPGVAEPC
                     LAIKDDVNLSYEYTRRWNMVAVVTDGTAVLGLGDIGPEAGMPVMEGKCVLFKAFADVD
                     AFPLCIKSKDVDEIVNTIKLISGGFGGVNLEDISAPRCFEIERRLKKECDIPIFHDDQ
                     HGTAIVVLAAMINALKVVKKDIKDIKVIVNGLGAAGTAITRLLMSMGLENVIACEIQG
                     AIYEGMEGLDSEKAAMAKITNPQKRKGMLKDIIVGADVFIGVSAPNILTPDMVKTMAK
                     DAVVFAMANPVPEIMPDLAKEAGARVVGTGRSDFPNQINNVLAFPGVFRGALDVRASD
                     INDEMKIAAAQAIADLVREDELNEDYIMPAAFDERVAPAVAKAVAEAARDSGVARI"
     gene            complement(5915..7015)
                     /locus_tag="VFD89_08865"
     CDS             complement(5915..7015)
                     /locus_tag="VFD89_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HZJ58326.1"
                     /translation="MDNNIKWIDFDTMERFMVDVFKGVGVPEEDAKTCADILIEADKQ
                     GIDSHGTNRLKPIYYDRINDGIQFAKTNFEIVREGPTTAVIDGHHGMGMVIGKRSMQM
                     AIDKAKKYGMGMVAVRNSTHYGIAGYFAKMAVDAGMIGITGTNARPSIAPTFGVENML
                     GTNPLTFGIPSDEEFPFMLDCATSITQRGKIEVYDKLGKTMPEGWVIDEDGNYQTDAG
                     KVLVDLVKGTAALTPLGGAGEDLGGYKGYGYATVVEILSAALQGGSFLKMLLGIENGK
                     KVPYALGHFFIAIDISAFIELEDFKKTTGDILRELRNSKKAKGHDKIYTAGEKEYETW
                     LYRKDRGVPVNEELLKELITLRDELNLTGYEF"
     gene            complement(7410..8201)
                     /locus_tag="VFD89_08870"
     CDS             complement(7410..8201)
                     /locus_tag="VFD89_08870"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HZJ58327.1"
                     /translation="MRHLRKYFTVFRISLENAFTYRSNLIGGLLFYSLFIFVFFNLWR
                     AIYQGGRVAGYSLVQMVWYLCMTELMVFGCRSSIFGQMNEDVKSGSIAYQLNRPYQYV
                     AYQFFNALGGVAFNFLIYGTLAIILGLVMVGPIPGFNFAVLPLALISVILGAVINFFL
                     MMALGLTAFRLEENTAFYLIYQKLVFMLGMFIPLELLPVWLQNIAKWLPFPYVAWAPA
                     RLIVAFDFGLFWEVIPMQLIWAVLAIILSMTVYSYGIRGIHAHGG"
     gene            complement(8198..9190)
                     /locus_tag="VFD89_08875"
     CDS             complement(8198..9190)
                     /locus_tag="VFD89_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZJ58328.1"
                     /translation="MYAIEAENLSKVYRTRTKEEGLGASFRALIKPVYKEVNAVHDIG
                     FTVKQGEIMAFIGPNGAGKSTTIKMLTGILHPTGGRIRVLGLDPVSDRQKLSYRIGTV
                     FGQKSQLWFHLPPTDSFALLGAIYDIDRAALKKRTQELTDLFGLGDLLNVPVRKLSLG
                     QRIRCEVAASLLHAPDILFLDEPTIGLDVVVKQGIRELIMRMNREQGTTIFLTSHDTG
                     DVEQVCRRAMVIDKGHIILDQSVKKLKYDYLNRKIIAVRFDTPPHLKAPVGITLEKLG
                     KTAARLSVDTRICPIDEAMRWLIGQGGVADITVEDPPMEEIITAIFNTAGGEAS"
     gene            complement(9514..11145)
                     /gene="hcp"
                     /locus_tag="VFD89_08880"
                     /gene_synonym="priS"
     CDS             complement(9514..11145)
                     /gene="hcp"
                     /locus_tag="VFD89_08880"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HZJ58329.1"
                     /translation="MNMFCYQCQETARGTGCAVRGVCGKTPEVSNLQDLLIYTLKGIS
                     EIVVKGDLNVKDLGMINHDVLNGLFITITNANFDDDSIEKEIKKMLAARDGLRAKAGD
                     LDLHDSATFTVSDRESMLKKAVTVGVLSTENEDIRSLREMIIYGLKGLAAYTHHALNI
                     GKEDYDLYAFVYEALAATIDDSLGADDLVGLTLKTGEYGVTAMALLDNANTSKYGNPE
                     ITEVNIGVRDNPAILVSGHDLTDLEQLLEQTQGSGVDVYTHGEMLPAHYYPAFKSYEN
                     FVGNYGNAWWKQIEEFASFNGPILFTTNCIVPPRSEEVKSRIFTTGATGYPGCVHIEA
                     DEEGKKDFSQIIEMAKGLKAPTEIEKGTIVGGFAHNQVFALADKVVEAVKTGAIKKFF
                     VMAGCDGRMKSRDYYTQFADRLPMDTVILTAGCAKYRYNKLDLGDIGGIPRVLDAGQC
                     NDSYSLAVIALKLKEIFELEDINELPIVYNIAWYEQKAVIVLLALLYLGVKNIHLGPT
                     LPGFLSPNVAKVLVEKFGIAGIGEVDEDIRMFMGA"
BASE COUNT         3002 a         2883 c         2407 g         3339 t
ORIGIN      
        1 acaccgtata acaaacttca atcaaaatat atgcactata tggaactatg cctaacaaat
       61 cccaggattt ccccataaaa attggtatca ttaataatat acaagaagga ttgtagtgca
      121 ttttatagat caacgattcc atctacaaag tttatcatac caataaatct cgtttccgta
      181 ttgttcatct ataatggata ttgatatccc cttaaccctc cctttcaatg ttttcattga
      241 ctagtttccc gggcctttga tatactttca ataaatacac ccccggtttg gcttactcta
      301 caggtattaa tcacaataca attatggagg gattttttat ggatatgcct atgaaggtta
      361 tacccggtta tttcaattca aagcccggta tacaggtggg gactaagatg caaggcccca
      421 taacccgtga tgaagaggga aatataaagt tggataagga tggggtcgca tttctccagc
      481 aaatggggat agaatgggta atgttgggcc atcgccaggt gcccgagcac accgtccagt
      541 gctataagga gcttaaagat gcactatcgg aggttgggct gaagattttc aggttggaga
      601 actatgaact tcataatatg cctgatatta cactggggct tgataatcgg gatgaaatgc
      661 ttgacaggta cctacaatac atatcggatt tgggcgaggc gggcattcat tacgctacct
      721 atgcacatat gggaaacgga atttggcgaa attatgaagg cggtacagtc cgcggcggtg
      781 cattgtccgg aggcttagac ctcaatgccc caaaccgttc catagccttt ggggattatg
      841 agtggcccct aagccacggc agaacctata gtgaggatga gctttgggaa aactacgagt
      901 attttataca acgggttgtt cccgtagcgg aaaattccga agtttatatc ggtatacacc
      961 ccgacgaccc acctgcctgt accatagccg gagtccccag gtgtatcttc agtagcctta
     1021 atggatataa gcgcgctttg gaaatggccg atagtcctaa tatcggggtg tgtctatgtg
     1081 tcggctgttg gctccagggg ggcgatgaga tgggctctac tccagaagaa tttattaaat
     1141 atttggggga aaacaagaag ctatttaaac ttcatcttcg caatgttact gctcccctgt
     1201 tcagtccggg cggttttaat gaaacctttc cagatgccgg ctactataat cttgtcaaca
     1261 taatagaagc cctggatgaa ataggttttg atggaatcgt cattaatgat cacctgatag
     1321 acatggtagg ggggcactac acctgtgagg cgtattttac ctcgtacctt aagggcgcgg
     1381 tagacgctat acaaaaccat cggaggaaat gatatgacac attaagctga ttatacttat
     1441 gtgtaggtaa tggctattgg aggtaaccta gctatcctgc ctatcaatag ccattattct
     1501 tattaaatgg atgggtctgg gtcagaggtc cgcaagagag atcaaatgtg tcgctgaccc
     1561 cgtccccgtg acacagtcac cagattaaag aacttgttta ccagttcccg gcttacatgg
     1621 cctgtcccgg caaactgggt gtttgccatt ccgcaggcca tgccggttct taggacatct
     1681 tctatggcct taccctgagt cagagctaca gcacacccgg cgatgaaaga atcccccgat
     1741 cctacggtgc tgatggtctc aatgggcggc gcctttaata taaaggcacc ttcatccgtg
     1801 gctgctatgc agccaccggc tcctaaagtg acacatacca gttcaatccc attttggtgt
     1861 atttcttttg aagcctctaa tatctcatat tccgaatcca tcttttttcc cattatggtc
     1921 tctaattccg tctcattggg cttgatcata aaagggcgat attcaaggct ctttaccagg
     1981 gtttgaccgc tggtgtcaag caataatctc ttcccaagcc cctttgaaat ctcaatcaat
     2041 ctcccgtaaa aatttacagc cacccccctg ggcagactcc ccgatatggt aacaatatcc
     2101 gcctttttgg cgatgttttt aaaatctatt agaaaccctt cctgctcttt tggggatatg
     2161 acgggacccc actccagcac ttccgtagag gtatccgaaa ttggatcgtt tatggctatg
     2221 catattcggc tatccccttc tatctcgata aaggcgtctc taatcccctg ctcatgtaat
     2281 ctggatctta taaattgccc gttaccaccg cctaagaatc ccatagccgt cacctcggct
     2341 cccagaatgc cggctaccct tgatacatta agcccttttc cccctgccgt tgcccacata
     2401 ccgctgggcc gaaatacctc ccctatggca aaggattcta cctcgtagac cttatcaata
     2461 gccggattta acgtaaccgt taaaatcatc atgtctcccc caaaaattaa attaaggcgt
     2521 tgcgccgatc cattatcttg atgtgtaata aaggtaatga gctattaaaa atcaatgtcg
     2581 gaagtagcgt ttattaatca ttaccgacat gattaactat atgttttacg acaaacattc
     2641 tacattggta cataggtgtc gtaaaacatt atccttaatt cgtatgttac gaaaaataca
     2701 ttattttaac gtaaaatccc atactccgct gaatggacct tggaaattta agatttaaaa
     2761 taccgtcttt aaaaacgcta actactcccc ttgataaagg tgtcttcggg gatttttagg
     2821 tactttagaa tctttttaat tgattcatta aggggccttg cataactcct ttcccaaggt
     2881 ctatccgggt agcatataac ccgaatcata acagcgtaga aaacatcata tataacacgc
     2941 tgtgtaggac gattcatctt ggtttttctg ggcccaataa tgtagtcatc atcctgcatt
     3001 aatccgcgcc ttacaacgta ctccgccagt gatagcataa ggatgcagat gagcatcagg
     3061 taagaaaatg cctctatccg ctcaggcgac tcaaggaaga ttgaattgac aaattgcggt
     3121 gatttcaatt gctgaaactt cttttctacg ctatcttggg ttttatattc cctaagcaat
     3181 gcttcgccag tcattccaag atcattggaa catagtacga aacagcaagc cttattaatg
     3241 gccttcgttg ttttttcttg gtttagaacg gggctaagct ctataaaata gcggtatcct
     3301 agtattttgt tatcttcacc ttttgcaggg cgaccacgct tgcggcgttc ttctcgtcgg
     3361 agggaaaaat tgatatcgtg atacttaagt tttttaaact ccttggaatt aatcttttgt
     3421 atctcacgat ttgcgtcgtc ctcgcaggcg aactcccgct tagtcaatac ttttgatact
     3481 ttcgtaatgc gttccatttc cttttttatt gctttatctg ccgtcttttc ttttgacgcc
     3541 tttaatgatg gggaatagca tactgtaagt ctgagctcat gttcgtgata gatgcaggtt
     3601 ttctctagca gactataggg tttcggctcg cctttagcat tgatgatttc tatgttctct
     3661 agggtatcaa ggatatctac tgcttcttcc atactttcct tggcgataag aacattatcg
     3721 gggatacggg ttatgagctt tatacctttt tccgtggata gatccaaatt ctccttggaa
     3781 aatagggcac tgtctgcaat gtagtaaaag ttacctttgt ctacattcaa attatgaagg
     3841 gtagcatcta aggcgctaat ggtatctgtg ttgtaggttt tgtcatcttt gttcccggaa
     3901 agtacagttg catctactac cagaccattg gcacagccaa taccaaattt aatttgtttt
     3961 ttatcttccc ttctatcttt gctatggcca aagtccattt ttatgacccc ttctgtacct
     4021 tcataggttt catacaagcc ccacatgacc ttggaagtgg tgtcaaaatt gatgtttgta
     4081 acctgtatgc catacagtgt aatggctttg cccgcaattt gtgaaaatat cttcttacag
     4141 ccagctccgt ggaaaagatc caggaaacca ccaaagcgat catcgttaat ttgctctagt
     4201 tttatcggat gatggaacaa accttctaga tccttgtact ggtaatattc gtgtagcctt
     4261 gataaaggca tatgatcatc acacatatta accatcatca tcatcgcatc gacaccataa
     4321 gggatatctg ggggacggcc attaacagag ccaagtgctt tattgattat ttcaggtact
     4381 cctaaagatt cgcataaggc cacgataaag ccctctttcc ctatcgtata ggtctcaaca
     4441 atgtttggat ccaccatagc gttttcctcc caaaactttt cactttaagg aggtattcga
     4501 cataaatctt gaaaaccctt ttggtaatta attacatgta aaatacagat ttaccaattt
     4561 tagttcagcg gagtatggga tatataacca atacaaacgg aaaatccgtt ttgaactatc
     4621 ccctcccctt gtacatatgc cggggataat atattgcttc tctttagatc aaaatttccc
     4681 ctaaggttat atcctagcta caccggaatc tctcgcagcc tcggcaaccg ctttggcaac
     4741 tgctggagca accctttcgt caaaggccgc aggcattata tagtcctcgt ttagctcatc
     4801 ttcccttaca aggtcggcta tagcctgggc cgctgcgatc ttcatttcat cgtttatatc
     4861 actggccctt acatcaaggg ctcccctaaa cacgccgggg aaggctaaaa cgttgtttat
     4921 ctggttggga aaatccgatc ttccggtccc cactaccctt gctcccgctt ccttggcaag
     4981 gtccggcatt atttcgggca ccgggttggc catggcaaat actacagcgt ccttggccat
     5041 ggtcttgacc atatccgggg tcagaatatt aggagcggat acccctataa acacgtctgc
     5101 ccccactatt atatccttaa gcataccctt cctcttttgg gggttggtta tcttggccat
     5161 agccgctttt tcagagtcca ggccctccat cccttcatag atggcgcctt ggatctcgca
     5221 ggctattacg ttttcaagac ccatgctcat gagcaatctg gtgatggcgg tacctgcagc
     5281 tccaaggcca ttgactataa cctttatgtc ctttatatcc ttcttaacga cctttagggc
     5341 gtttatcata gccgccagga ccacgatggc agttccgtgc tgatcgtcgt gaaatattgg
     5401 tatatcacat tcttttttaa gccttctctc gatttcaaag cacctagggg cggatatgtc
     5461 ctctaggttt acgcccccaa acccccccga aatcagctta atggtattta ctatctcatc
     5521 aacgtccttg gactttatac ataagggaaa ggcatccacg tcggcaaagg ccttaaacag
     5581 cacgcattta ccctccataa caggcatccc ggcttcggga cctatgtctc ccaatcccag
     5641 gaccgcagtt ccatcggtga caacggccac catattccac cttctggtat attcatagga
     5701 caaatttaca tcgtccttta tggccagaca aggttctgcc accccgggtg tgtaggccag
     5761 ggaaagatct ctgctgttat ctaccttaac ggtattttta acctcgattt ttcccttcca
     5821 ttccccgtgc agctttaaag cttcactctt tatatccatt ataggttccc tcctgcatct
     5881 ttcattgaag aagttcaaaa aacatcttta gccgctaaaa ctcatacccc gtaaggttca
     5941 gttcatccct cagcgtaata agctccttta acagctcttc gttgacaggc actcccctat
     6001 ccttcctata gagccaggtt tcatattcct tttcccccgc ggtgtatatc ttatcatgac
     6061 ccttggcctt cttggagttt ctaagttccc ttaagatatc gccggtagtc ttcttaaagt
     6121 cctccagctc tataaatgcg gagatgtcga tggcgataaa gaagtgtccg agagcatagg
     6181 gtaccttttt gccgttttcg atgcccagca gcatcttgag gaaactgccg ccctgcaggg
     6241 ccgcggatag aatctctact accgttgcgt agccatagcc cttgtagccg ccaaggtctt
     6301 cacctgcgcc gccaaggggt gtgagagccg ccgtgccttt aacaagatcc accagcacct
     6361 tcccggcgtc ggtctgataa tttccgtcct cgtctatgac ccagccttcg ggcatggttt
     6421 tacccagctt gtcatagact tcaatcttac cccgctgggt gatggatgtg gcgcagtcca
     6481 acataaaggg aaattcttcg tcactgggga taccaaaggt aagggggttg gtgcccagca
     6541 tattctctac cccaaaggtg ggtgctatag agggtctggc gttggtaccg gtaatgccga
     6601 tcataccggc atctacggcc atcttggcaa aatagcctgc gataccgtaa tgggtggagt
     6661 ttctgacggc taccataccc atcccatatt tttttgcttt atcgatggcc atctgcatgg
     6721 atctcttgcc tatgaccata cccattccat ggtgaccgtc tatcaccgca gttgttgggc
     6781 cttctctgac tatctcaaag ttcgtcttgg caaattggat tccgtcgttg atcctatcgt
     6841 aatatatcgg ttttaaccga ttggtgccat gggagtctat gccctgttta tccgcttcta
     6901 tgagaatgtc cgcacaggtc tttgcatcct cctcaggaac ccctaccccc ttaaagacgt
     6961 ctaccataaa cctctccatc gtatcaaagt cgatccattt aatgttgttg tccatgaata
     7021 tcaccctttt tatgaatttt ttaattacat ataagcagaa acaaaggttt aaccatgtag
     7081 atccggattc caattccgcc gccgttagga gcacaaaaaa atcgtcctaa aacaggggct
     7141 gttattaccc cctccctttt agcacgactc tcaatctccg catggctttt attctgttat
     7201 agataaacaa tcacggacat aaaatttatc accatgaaat tatacagacc cattttaatc
     7261 atcacgccgg taaatttata tgtcgataaa attagtttcc atatcgttta tccgtaatta
     7321 aactatatca caaagcccat ttccatacaa gttaaaatac agtcccataa gccttaacat
     7381 cttccttaaa ttctccaaaa tattaccccc taaccgccat gggcatggat gcccctaatc
     7441 ccatagctat ataccgtcat ggatagaata atggcaagca cagcccagat aagctgcatg
     7501 gggattacct cccaaaacaa gccaaagtcg aaggctacga tcagccgggc aggagcccat
     7561 gcaacatatg gaaagggcag ccattttgcg atgttttgga gccataccgg caacaattca
     7621 aggggtatga acatccccag cataaaaacc aacttttgat agatgaggta gaaggcggtg
     7681 ttctcttcca ggcgaaaggc agtaagcccc agggccatca tcagaaagaa attgatcacc
     7741 gcacccaaaa tgacagagat cagagccaag ggtagtacag caaagttgaa tccgggaata
     7801 ggccccacca tcaccagccc caatattata gctaaggtgc cgtagatcaa aaagttaaat
     7861 gccaccccgc ccagggcgtt aaagaattgg tatgccacat attggtatgg cctattcagc
     7921 tggtatgcga tgctgccgga ttttacatcc tcgttcatct ggccaaaaat gctgctgcgg
     7981 catccaaaga ccataagctc ggtcatacac agataccaca ccatctgcac aagggagtat
     8041 cctgcgaccc ggcctccttg gtatatggcc ctccagaggt taaagaatac gaagataaac
     8101 agggaataaa acaggaggcc gcctattaaa ttactgcgat atgtaaaggc gttttccagg
     8161 cttatccgaa acaccgtgaa gtattttcga aggtgcctca tgatgcctca cccccggcgg
     8221 tattaaagat ggctgttatg atctcctcca tgggcgggtc ctccacagtg atatcggcga
     8281 cgcccccttg gcctataagc cacctcatgg cctcgtctat ggggcatata cgggtgtcta
     8341 cggatagccg ggccgctgtc ttgcccaact tttcaagggt gattcccacc ggggccttaa
     8401 ggtgcggcgg cgtatcaaag cgcacggcga tgatcttgcg gttcagataa tcatatttta
     8461 gcttcttaac cgactggtcc aatatgatat gccccttgtc tatgaccatg gcccgtctgc
     8521 agacctgttc cacatccccg gtatcatggc tggtgagaaa aatggtggtc ccctgttccc
     8581 tattcatgcg catgataagc tccctgatgc cctgtttgac taccacatcc aggccgatgg
     8641 tgggctcatc caggaacaga atatcgggtg catggagcaa ggaggccgca acctcgcagc
     8701 gaatcctctg ccccagggac agcttcctga ccggaacatt caataggtcc cctagtccaa
     8761 agaggtccgt cagctcctga gtccgctttt ttaatgcggc cctgtcgata tcgtatatgg
     8821 cccccaggag ggcaaagctg tctgtggggg gcagatgaaa ccatagctgg gatttttgac
     8881 cgaacacggt ccctatgcgg taggatagtt tttggcggtc agagacaggg tccagaccca
     8941 atacccgtat ccgtcccccg gtgggatgga gtatgcccgt cagcatcttg atggtggtgg
     9001 attttcccgc accgttggga cctataaagg ccattatctc cccttgtttt acagtaaaac
     9061 ctatgtcatg gacggcattg acttccttgt agacaggttt aatgagggcc cggaaggaag
     9121 cgcccaatcc ctcctcctta gtcctggtgc gatagacctt actgaggttc tccgcttcta
     9181 tggcgtacat gatcccctcc cctttccttt ccaccaacat agcggtgaga atggattttg
     9241 tatacacaaa caccataccg gtgggaatgg attctgtata tacaaacgcc gcatcgatgg
     9301 tgtattcatc tctgcggcgt ataatacaag tatataattg tcacccaaac aacagctgca
     9361 ctacggcaat ataccctata catcgcccgg ttaaatcggc ccggcactat atagtatata
     9421 tgggaccaaa ctgcatgctg cccgggtaat aaacattata tggtatctac acatataagt
     9481 caacaaaaat aagagaggta ctgggagcta ttattatgcg cccatgaaca tccttatatc
     9541 ctcgtcaacc tcaccgatac cagcgattcc aaacttctct accagaacct ttgcaacatt
     9601 gggtgataaa aatccgggta gggttggccc taagtggata tttttgacgc ccaaatacag
     9661 tagagccaat agcacgataa ccgccttttg ctcataccag gcaatattat agactatggg
     9721 aagctcgttt atatcctcaa gctcgaagat ctccttaagc ttaagggcga taaccgccaa
     9781 ggaataggag tcattgcatt ggcctgcatc cagtaccctc ggtatgccac cgatatcccc
     9841 tagatccagt ttattgtacc tatacttagc acatcctgcc gtgaggatta cggtatccat
     9901 aggaagcctg tccgcaaact gggtataata atccctggac ttcatccttc cgtcacagcc
     9961 tgccattaca aagaacttct ttatggcgcc ggtctttacc gcctcaacaa ccttatcggc
    10021 caaggcaaag acctgattgt gggcaaagcc gcctacgatg gttccctttt caatctctgt
    10081 gggagccttt aggccctttg ccatttcaat tatctgagaa aaatccttct tgccctcttc
    10141 atccgcttct atgtgaacac atccgggata tcccgttgcc cctgtggtaa agatcctgga
    10201 ttttacctct tcgctcctgg gaggaactat gcagttggtg gtaaatagaa tgggaccgtt
    10261 aaaggatgcg aactcctcta tctgcttcca ccaggcattt ccgtagttac ccacaaagtt
    10321 ttcgtaactt ttaaaggccg ggtaatagtg ggcaggcagc atctcaccat gggtatatac
    10381 gtccaccccg ctgccttggg tttgctccag cagttgttca aggtccgtca aatcatggcc
    10441 cgataccaag atggcagggt tgtctctgac gccaatattt acttcggtga tctcgggatt
    10501 tccatacttt gaggtgttgg cattatccag cagtgccata gccgtaaccc cgtattcacc
    10561 tgtctttaga gttaggccca ccaaatcatc ggcgcctaag ctgtcgtcta tggtagcagc
    10621 taaagcctcg tagacgaagg catacagatc gtaatcttcc ttacctatat taagggcgtg
    10681 gtgggtatag gcggccagtc cctttaatcc gtatatgatc atttctctta gggatctgat
    10741 atcctcgttt tccgtggaca ggacgccaac ggtcactgct tttttaagca tggattctct
    10801 atccgatacg gtaaatgtag cggagtcatg aagatctaaa tcgccggctt tggcccttaa
    10861 tccatctctg gcagccagca tcttttttat ctccttttcg atggagtcat cgtcaaagtt
    10921 agcattggta atagttataa aaaggccgtt taaaacatca tggttgatca tgcccaagtc
    10981 cttgacatta agatcacctt ttaccactat ttcggatatg ccctttaggg tgtagatgag
    11041 aagatcctgt aggtttgaaa cttcgggcgt cttaccacat accccacgaa ccgcacaacc
    11101 ggtaccccta gcggtctctt gacattgata acaaaacata ttcattttca tttcccccct
    11161 gaaattaatc ccatgggata ttccacagga cattggtttt aatatattta aaggtaggac
    11221 gaatccccct tcatatcgta ttgattaatt cgttgatcta tatgttttac gacaaacatt
    11281 ctacattggc acacaggtgt cgtaaaacat tatccttaat tcgtatgtta cgaaaaacac
    11341 attatttaaa cgtaaaatcg gataattaaa ccgattttac aatgaaaggt ctttttcgta
    11401 acatatctag tccatcatct tatggtgtga tgacaatatg gaagccatta cttttttatc
    11461 ggcaattttt aggggcagac gggagcgcct gccccttgta ctaagcattc aatttcgctt
    11521 ccagttggtc caataaatcc atatatcttt gttggatatt atcttccctg gccctctctt
    11581 caaagatgat acactcttca tttatttccc ttagggtatc ctctgccagg t
//
LOCUS       DASRNX010000225         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_469338,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000225 DASRNX010000000
VERSION     DASRNX010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469338"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..673
                     /locus_tag="VFD89_08885"
     CDS             227..673
                     /locus_tag="VFD89_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HZJ58330.1"
                     /translation="MQISTKGQYGLRAMVDLAIYSSNAPIPLSSIANRQSLSLGYLEQ
                     VFSSLRKAGLVKSVKGAQGGYTLGDDQSNITVGDVLRSLEGDLNVVSERDEDEESKNS
                     IEHCLKTMVWGRIDEALNELADSITLGDLATESQRLNKSLANMYYI"
     gene            687..1628
                     /gene="cysK"
                     /locus_tag="VFD89_08890"
     CDS             687..1628
                     /gene="cysK"
                     /locus_tag="VFD89_08890"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HZJ58331.1"
                     /translation="MAKIAKRLTDLIGNTPLLELSNYSKVEGLERPVVAKLEYFNPAG
                     SVKDRIGYAMIKDASDRGLINDDTTIIEPTSGNTGIALAFAAASLGYKLIIIMPDTMT
                     KERRSLIRALGAQLVLTPGAEGMKGAIKKAEELAKENPNSFIPQQFQNLANPKIHRET
                     TALEILRDTDGDVDVFVGGVGTGGTITGVGEVLKDKNPDIKVVAVEPADSPVLSGGEA
                     GPHAIQGLGAGFVPDILNTEVIDEIIQVKNEDAIETAKLLAKTEGLLVGISSGAAAYA
                     AAQIAKRPEFRGKRVVVVLPDTGERYLSNPLLFPDQE"
     gene            complement(1736..2218)
                     /locus_tag="VFD89_08895"
     CDS             complement(1736..2218)
                     /locus_tag="VFD89_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HZJ58332.1"
                     /translation="MDKKDRQFHTFRGYQIIKQEKTRLTPGMEDYLEMIYRHCRNEGY
                     VRIVQLSELLNVQAPSVTRTVQRLAESGLVDYEKYGLVKLTEEGERIGQILLERHILI
                     ENFLGKIGVRDTLLRDTEMIEHNISVEALRSIEFFNNYLDDNPHILKGFEEYKRSRGC
                     "
     gene            2352..2588
                     /locus_tag="VFD89_08900"
     CDS             2352..2588
                     /locus_tag="VFD89_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HZJ58333.1"
                     /translation="MTKKYIPLSALKLGNAARVWELTMGGSIRRRMLDLGLVRGTRIE
                     AINRSPFGDPVAYSFRGAIIALRSEEASGIIVEM"
BASE COUNT          807 a          587 c          655 g          705 t
ORIGIN      
        1 gggctaaaag gatttcgatc aaatctagaa ggaaccactt ttatgtcgca ataaatacta
       61 gctttgatgc cattagagcc cacacctata aatcaatttc ccttaactag tctaagttaa
      121 cgggatgggt tttcaatcat tttccttaat tttcttgaca aatataaggg ttcatgctaa
      181 tatatattaa accaatacgc atactatgct tatggaggtg attaggatac agatctcaac
      241 taaagggcaa tatgggttaa gggcgatggt ggacttggca atttattcgt caaacgcccc
      301 tatccccctg agcagcatag caaataggca gagtctgtct ttggggtatc tggaacaggt
      361 tttttcttct cttcgaaagg caggtctggt taaaagtgtt aaaggtgcac aaggcggata
      421 caccctgggt gatgatcagt ccaatattac cgtaggtgat gtcttaagat ccttagaagg
      481 cgacttaaat gtagtgagtg aaagggacga ggatgaggaa tccaaaaaca gcatagaaca
      541 ttgtcttaag acaatggtat ggggtaggat tgacgaggca ctgaatgaat tggcagattc
      601 aataaccctc ggcgaccttg cgacggaaag tcaaaggctg aataaaagcc tggcaaatat
      661 gtattatata tagaggagcg aaaactatgg caaaaatagc gaaaagatta acggatctga
      721 taggaaatac gcccctgttg gagttatcaa attatagcaa agtggagggg ctggaaagac
      781 ctgtagttgc aaagttggag tatttcaacc ctgccggcag cgtaaaggac agaattggtt
      841 acgctatgat aaaggatgca tccgataggg gattgatcaa tgatgatacc actataattg
      901 agcccaccag cggcaatacc gggatcgcat tggcttttgc ggctgcatct ttaggttaca
      961 agttgatcat tataatgccc gataccatga cgaaggagcg acggagcctt ataagggcat
     1021 taggtgccca attggtgctg actccaggcg cggaagggat gaaaggtgcc ataaaaaagg
     1081 ctgaggagct ggccaaagaa aaccccaatt catttattcc tcaacaattt caaaacctgg
     1141 cgaaccctaa aattcatagg gaaaccactg ccctggagat cttgagggat accgacgggg
     1201 acgtagacgt ctttgtcggc ggggtaggaa cgggaggtac catcacaggt gtgggtgaag
     1261 tcctcaagga caaaaatccc gatatcaagg ttgtagcggt agaacccgcc gattcccccg
     1321 ttctatcggg tggagaggcc ggtccccatg ccatacaagg gcttggcgcc gggtttgtgc
     1381 ctgacatctt aaatactgaa gtcatagacg agataataca ggtaaaaaat gaggatgcta
     1441 tcgaaaccgc caaactgctg gccaaaaccg aaggcctatt ggtaggcata tcctcaggag
     1501 ctgcagccta cgccgcggcc cagatagcta agaggccgga atttaggggc aagagagttg
     1561 tggtagtgtt gccggatacc ggcgagagat atctttcgaa tccgttgctt ttcccggacc
     1621 aagaatgaga cggaagaatt aaaaatttct cacagactaa catcaaaaaa ataatccatc
     1681 cggccgatgt tttcggccgc actaatgaaa ctccatatta agagaagccg ggactttagc
     1741 atccccggct tctcttatat tcctcaaacc ccttcaagat atgggggtta tcgtctaaat
     1801 agttgttgaa aaattcgata cttcgaaggg cctccacgct gatattatgc tctatcattt
     1861 ccgtatccct taaaagggta tcccttaccc ctattttacc cagaaaattt tctataagga
     1921 tatggcgttc caacaggatc tggcctattc tttccccctc ctcggtgagt tttaccagcc
     1981 catatttttc gtagtccaca agtcctgatt cagctaaccg ctgtactgtt cgggtaacgg
     2041 acggggcttg aacgttgaga agctccgaga gctggacaat ccgcacatac ccttcgttcc
     2101 tgcaatgtct gtatatcatt tccagataat cctccatgcc cggcgtgagc cgggtctttt
     2161 cctgtttaat tatttgatat cccctaaagg tatggaattg tctatccttt ttgtccattt
     2221 ttttcaccac cttgaacaca aaacctggcc gattcatata ttagccttag gtaactcttt
     2281 tggtcatacc taagtctatg aaaaaggacg ccaaaaatat aaccaatata tcgttaaagg
     2341 aggattctgt gatgacaaaa aaatatatcc ccctaagcgc acttaagctg ggcaatgcag
     2401 caagggtatg ggaattgacc atgggcggca gcatcagaag gcggatgctc gatctgggct
     2461 tggttagggg cacaagaata gaggctataa acaggagtcc cttcggggat ccggtggcat
     2521 attcattccg gggagccatt atcgcccttc gcagcgagga ggcttccggc attatagtcg
     2581 agatgtaaaa agtctaagac atcctagata agcaacttca actaaaaatt tatcgccatg
     2641 tcttgcatag aaccactgca actatcatgc cgataaatct agttttcgta agcttatcta
     2701 ttattgaggg gggaaagaaa tgggattaac gtctcaatct accgggctta actc
//
LOCUS       DASRNX010000226         4280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_681951,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000226 DASRNX010000000
VERSION     DASRNX010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681951"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1817
                     /locus_tag="VFD89_08905"
     CDS             <1..1817
                     /locus_tag="VFD89_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HZJ58334.1"
                     /translation="LIEFTPTFLDGISKEIINQYWFPLDWKKDPTISSMLVVLDSIHD
                     KFRDVDNIWGKLKEKTITFYFLAIRDMGLTDELYIKMNSRGKPLTQFEHFKAELERKF
                     SLVDKEISQRILSKIDREWTELLWQYRDGDNVMDDEFLRYFKFICDIICYKNGDSPQG
                     KSSDEFDLLNEYFSGNKDDIIRNIGIFEDYFDCWCSLDNEAPGEFLKRFISHEHTAGK
                     IKIDNRDEIDIFKDCLKKHSTISGRRRAFPLNRIVQLYGIICYLLNRSEISEAQFARR
                     LRIINNLVMNSEDEISDSESRSSGNRMPAILKQVDYIMKTGLIDTGIENNFSAIQLTE
                     ELEKIEWLEDNPDRANQLFALEDHGLLQGQIGILGLENFEYFERFKDLFECDRDKVDC
                     ALMAIDFYGQQEQNGWRYQLGCSAIDSAWRGLFHKSRNRGFERTKKTLIKLLSIQEEV
                     NDGLLDRVIAEYIDYCEGNSVYTWRYYYIKYPVFRPRRYGKYFNEDPDKNPYMFGVMA
                     TQSKLSSNSYMPFLKEADPKNISKDDYGRRLIHGDSYIEPLADGFIIKSIEDDSLISK
                     ITISQNEDGIDTENRIEKLKAHLVPGGIFRLTSEDNIS"
     gene            1892..2938
                     /locus_tag="VFD89_08910"
     CDS             1892..2938
                     /locus_tag="VFD89_08910"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HZJ58335.1"
                     /translation="MRHTNVSIRGERILNIEENRPKWHLAALGELLIDFTPVGKSRQG
                     GALFERNPGGAPANVLVVASKLGLSTAFIGKVGQDAFGEFLKDTLVKQGVDTKGLIMT
                     TEANTTLAFVHLDEGGDRLFSFYRNPGADMLLKESEVDEGILKESSIFHFGSVSMTKE
                     PARRATLKAVERAVALGKTISYDPNLRPPLWDSEREAKEIISQGLPFADILKISEEEL
                     EFLTDQKDPDIGSRLLYEKYKTSIILVTLGPDGCFYRSGEIAGYSPGFQVSTIDTTGA
                     GDAFMGGFLYQFIKSGKGVAELDGRELGEAVKFANAMGALTTTKRGAIPAIPSMEEVS
                     KTCLEGVSSDIKEC"
     gene            3166..4143
                     /locus_tag="VFD89_08915"
     CDS             3166..4143
                     /locus_tag="VFD89_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZJ58336.1"
                     /translation="MKRVLLTNTDMHVSPLCIGTVNYGTSIDPKDAKTQLDAYVELGG
                     NFIDTAHVYGDWVPGPRGRSEKVIGQWLNETGLRENVIISTKGAHPDISDVNVSRVNP
                     EAIEKDLNESLERLKTDYIDLYFLHRDNPNVPVVEVLDALEKARTAGKIRYYGCSNWS
                     LSRIIEASNYAKEQNISGFVCNQLMWSLADVNYKGIEDKTLVLMDEDTYEYHKETGLN
                     AMAYTAVAKGYFSKLQRGESVSEKISSRYDTPSNQQIFDEIVKTASRLNADIIQVELA
                     FLMHQDFASIPIVSFSSLEQLEQAMKSCELELDEGTISKLRSMKNYVIE"
BASE COUNT         1375 a          736 c         1040 g         1129 t
ORIGIN      
        1 ggctgataga atttacgccg acattcctag atgggatttc aaaggagata attaatcagt
       61 attggttccc gttggactgg aagaaggatc ctactatctc ttcgatgctg gtagttttag
      121 attcaatcca tgataaattc cgtgatgtag acaacatatg gggaaaatta aaggaaaaga
      181 cgattacatt ctattttctg gccataaggg atatgggatt aacggacgaa ttatatataa
      241 agatgaattc aaggggtaaa cccttgaccc aatttgaaca ctttaaggcg gaactggaaa
      301 gaaaattttc gctagtggat aaagagatct cccaacgtat actaagcaaa atagacaggg
      361 aatggacaga gttgttatgg caatatcgtg atggagataa tgttatggac gacgagtttt
      421 taagatattt caagtttatc tgcgatatta tttgctacaa aaacggtgac agcccccagg
      481 ggaaaagcag cgatgaattt gacctactaa atgagtattt ctccggcaat aaggatgata
      541 ttataaggaa catagggatc tttgaggatt attttgattg ttggtgtagc ctggacaatg
      601 aagcccccgg tgaatttcta aaaagattta tatcccatga acacacagca gggaaaatta
      661 aaattgataa ccgggatgag atcgatattt ttaaggattg tctcaaaaaa cactccacta
      721 tctcaggcag gagaagggcc tttcccttaa acagaatagt gcagctatat ggtattatat
      781 gctatcttct taatagatca gagatcagtg aggcccaatt tgccagaagg cttagaataa
      841 tcaataatct agtaatgaat tcggaagacg agataagcga tagcgaatcc cgttccagcg
      901 gcaataggat gcctgcgatc cttaaacagg tggactatat tatgaaaaca gggcttatcg
      961 ataccggcat tgaaaacaat ttctctgcta ttcagctgac agaggaacta gaaaaaatag
     1021 aatggctgga agacaatccc gatagagcaa accagctgtt tgcccttgaa gaccatggtc
     1081 tattacaggg tcaaataggt atattaggtt tagaaaactt cgaatatttc gagaggttta
     1141 aggatctgtt cgaatgtgat cgtgacaagg tggactgtgc gttgatggca attgattttt
     1201 atgggcagca agaacaaaac gggtggagat atcaattagg atgcagtgcc atcgacagcg
     1261 catggagggg attgtttcat aaaagcagga ataggggctt tgaaagaacg aagaagacgt
     1321 taataaaatt gctgtctatc caagaggaag taaacgatgg attgcttgat cgggtaatcg
     1381 cagaatatat agattactgc gaaggaaatt cggtatacac ttggaggtac tattacatta
     1441 agtatccggt atttcgtcct aggcgttacg gcaagtattt taatgaggac cctgataaaa
     1501 atccatatat gtttggagtt atggcaaccc aatctaaact gtcatctaat tcgtatatgc
     1561 cctttttaaa agaggctgat cctaaaaaca tatccaagga tgattacggc agaagactaa
     1621 tacatgggga tagctatatt gagcctttgg ccgacggctt tataataaaa tcaatagagg
     1681 atgacagtct gatttccaaa ataacgatat cccaaaacga agacgggata gacaccgaaa
     1741 accgaatcga aaaactaaag gcccacctag tgccaggggg aatatttagg ttgacatcag
     1801 aggacaacat atcataagaa gagttcgtga tggaattacc gaattggatc tttatggggg
     1861 ttaagctttt gttgttaaag aaagaggata tatgagacat acaaatgtaa gtatcagagg
     1921 tgagcgaatt ttgaacatag aagagaacag gccgaaatgg catcttgctg cgctgggcga
     1981 attattgatt gattttacac ccgtgggaaa atcccggcag gggggagccc tatttgaaag
     2041 aaatcccggg ggagcgcctg ccaacgtgct ggttgtggct agcaagcttg ggctctccac
     2101 cgcgtttata gggaaggtag gacaggatgc ttttggcgaa tttttaaagg ataccctggt
     2161 taagcaggga gtggatacga agggtctgat tatgaccact gaggccaata ctactctggc
     2221 ttttgtccac ctagatgagg gcggcgatcg attattcagc ttttatcgaa acccgggggc
     2281 agatatgcta ttaaaggaaa gtgaggttga tgaaggaatc ctaaaggaat catccatttt
     2341 ccatttcgga tcggtctcca tgaccaagga gcccgcacga agggcaactt taaaagcggt
     2401 agaaagagcc gtagcattgg gcaaaactat ttcctacgat cctaatttga ggccgcccct
     2461 gtgggatagc gaaagggaag ctaaagagat cataagtcaa ggcctgccat ttgcggatat
     2521 cttaaagata tcggaagagg agcttgaatt cttaaccgat caaaaggatc ctgatatagg
     2581 ttctaggctc ttatacgaaa aatataagac ttcgattatc ctggttacat tggggcctga
     2641 cggatgtttc tatcgcagtg gggaaattgc cggttattcc cccggctttc aggtgagtac
     2701 tatagataca accggagcgg gtgacgcctt tatgggtgga tttttgtatc agttcattaa
     2761 gtccggtaaa ggtgtagctg aattggatgg gcgggaacta ggggaagcag ttaaatttgc
     2821 caacgccatg ggagccttaa ctactaccaa aagaggggcg ataccggcta taccatccat
     2881 ggaagaggta agcaaaacgt gcttggaggg cgtttcgtct gacatcaaag aatgctaagg
     2941 ggacgtttcg tctgatatca aagaatgaca tggtaatctc gccttgccaa gggaatattt
     3001 cgtttgtcac caaataccaa tagggacgta attaccacaa cggttggatt cttacagaca
     3061 attcatattt agtgttttag tacatatggt tattggtata ataaaggcat agccagaaga
     3121 cagagcaaaa tagtatatgc tgaaattata tgaggaggca ctatgatgaa gagagttttg
     3181 ctaaccaata ccgatatgca cgtatcacca ctatgtatag gtactgttaa ttatggtaca
     3241 tctatagatc ctaaggatgc aaagacccag cttgatgcat atgttgaatt gggcggaaac
     3301 tttatagaca cagcccatgt ctatggtgac tgggttcccg gaccaagggg aagaagtgaa
     3361 aaagttattg gccaatggct taatgaaacc gggcttcgtg agaatgttat aatcagcaca
     3421 aagggggcgc atcctgacat atcagatgtt aacgtttcca gggtcaatcc tgaggccata
     3481 gaaaaggatc ttaatgagag cctggagcgt ttaaaaactg actatataga cctgtatttc
     3541 ttgcatcggg ataatccgaa tgttccggta gtagaggttt tggatgcatt ggaaaaagcc
     3601 cgaacggcag gcaaaatccg atactacggt tgttcaaact ggtccctttc acgaataatt
     3661 gaagctagta actatgccaa ggagcagaat atatcaggct ttgtatgtaa tcagcttatg
     3721 tggagcttag ccgatgttaa ttataagggg atagaggata agacccttgt attgatggat
     3781 gaggatacct acgagtatca taaggaaact ggcttaaatg caatggcata taccgctgtg
     3841 gcaaaagggt atttttctaa actgcagcgg ggagaatctg tatcggaaaa aatatcgtct
     3901 agatatgaca caccttcaaa tcagcaaatt tttgatgaaa ttgtaaaaac agcaagtcga
     3961 ttaaatgcgg acattattca agttgaatta gcctttttaa tgcaccaaga ctttgcttct
     4021 atacccatag tgtccttcag tagcttagaa caattagaac aagcaatgaa aagctgtgaa
     4081 ctggagctgg atgaagggac gataagtaaa ctccgcagta tgaaaaatta tgtgatcgaa
     4141 taaattggat atcttgtatg taatgttatg aagccgtttc gatcttatga tgccaaggag
     4201 gcgtttagtc tgaaaccaaa aacgaactgg cagtcccacc ttaccccaga gagtattaag
     4261 ccataccaag gtgcttatat
//
LOCUS       DASRNX010000227         4731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_96838,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000227 DASRNX010000000
VERSION     DASRNX010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96838"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1412
                     /locus_tag="VFD89_08920"
     CDS             123..1412
                     /locus_tag="VFD89_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZJ58337.1"
                     /translation="MKITDVKAAKIGTTIVIRVVTDKDGIDGYAQIESNKDYVMPLIP
                     YFKSMIIGHDPTDVESVLRQMRRSAAFKPWGKVFSSIEMALWDIAGKEAGIPVYKLLG
                     GKVRDKVRVYNGSFLDPNPPHEVADTPEKLGENILHLNEIPEGFTIVKVAAFWHMRNF
                     YGEGDAYNTYYPEGEGKGNRRGGYTEYLKMMNENKTFSPQLNKPVTEGSLLTEKGLKA
                     SIDFVERLKKVVGDKVGLAFDIGPGFMPGDVIRLAQALEPYNIMWLEDSIAGDYTPYT
                     MASVYRDITTSTSTPIHTGEQIFLRQNFMDLIETRAVDVVGPDPADVGGIKELKWIAE
                     YADLHGILMAPHGTFNGVFGMAALTHVCSTMPQNYVAYEFPGANPGFWLDIIEGLPNN
                     YLRDGHVKVWDAPGIGITFNVNKASKYLSPEDKDFFR"
     gene            1783..2928
                     /locus_tag="VFD89_08925"
     CDS             1783..2928
                     /locus_tag="VFD89_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZJ58338.1"
                     /translation="MAMTSRERLITVIEGRLPDHVPVAPDTSNMIPARLTGKPFWDLY
                     LFQDPPIWKAYLDCVKYFGFDSLMDGYVSIDFDDLPLEDDGYTYEEVIVYRSPDRIVT
                     QKYREERGKKIWADTVDVYYIADPPTMGLDPHSISLPSIPDHYEPIGEIRQWPKGEEL
                     LKYVKEEMGDHGLVGVRCGVTKLIESEWEIYDYFDNPDKYFEKRDRYLEHYVKKFHRL
                     MELECKPDFICTGNSGTLIHQTPQMFRDLALPIVKEITALCKEYGIPSHVHSCGPQKE
                     LVKIMAEETDLTIIDPLEIPPMGDCDLGEIKRLYGDRLVLKGNIHTTEVMLRGTTADV
                     AEAAKRCIDQAAEGGRFILSTGDQCGRDTPDENIFTLIEVAKTYGRY"
     gene            3269..4453
                     /locus_tag="VFD89_08930"
     CDS             3269..4453
                     /locus_tag="VFD89_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZJ58339.1"
                     /translation="MRKLQLELSQDIIINNCRREQVTLSEYACKSIRSDRYRDEEIPD
                     LKNIRPSFFHDTDRIIHSRAYTRYIDKTQVFYLFDNDHITHRVLHVQLVSKIGRTIGR
                     CLRLNEDLIEAISLGHDLGHVPYGHNGERYLNEICQKRGIGYFIHNAQSVRALMELEN
                     GGKGLNLTLQVLDGILCHNGEYLNQAYEPDKNKDWDKFMDEYTKCWQIKNYDKQIRPM
                     TLEGCVMRISDVIAYIGRDIEDAIVVGLIKREDIPDEVTKVLGNNNRQIINTLIIDLL
                     NNSYDKNYLTFSDDVFKALDTLFDFNYEHIYRNPQKATQDGKIENMFHYLFDVYLDDI
                     ENNKESSSIYKYFLDEKDEDYRKKNRNERIVIDFIAGMTDDFFNNQFKERIFPSKFGM
                     TI"
BASE COUNT         1481 a          872 c         1107 g         1271 t
ORIGIN      
        1 gtgtatcaga agagccgtat gcgggaaaac cgcacgtacg gttctgtgag gggcaataga
       61 cattcccttc acgatataaa tattttgaaa ggagtgtcga gtttgtctac tcgacgagaa
      121 tcatgaagat caccgatgta aaggctgcga aaattggaac aaccattgtt ataagggtgg
      181 taacggacaa agacggcata gacggttatg cccagataga aagcaataaa gattatgtta
      241 tgcccttaat cccctatttc aagagcatga ttattggaca cgatcccaca gatgtggaaa
      301 gcgtcttgcg tcaaatgcgt cgctcggccg ctttcaagcc ctggggcaaa gtgttcagct
      361 ccatcgagat ggccctgtgg gatattgcgg gcaaggaggc aggtattccg gtctataagc
      421 ttttgggcgg aaaagttcgc gacaaggtca gggtatataa cggcagtttc ctggatccca
      481 accctccgca cgaagtcgct gataccccgg agaagctggg tgaaaacatc cttcacctaa
      541 acgagatacc ggaaggcttt actattgtta aggttgcggc cttctggcat atgaggaact
      601 tctatggtga aggcgatgcc tataatacct actatcccga gggtgaaggt aagggaaacc
      661 gcaggggcgg ctacacggaa tatcttaaga tgatgaacga gaacaaaacc ttcagccccc
      721 aattgaataa gcctgttacc gagggcagcc tgctcaccga aaaaggtctg aaagccagta
      781 ttgattttgt agagcggctg aaaaaggttg tgggagacaa ggttggtctg gcatttgaca
      841 ttggtccggg atttatgccc ggggacgtga ttcgcctggc acaggcactt gagccatata
      901 atattatgtg gcttgaagat tccatcgccg gcgactatac gccatatacc atggcttctg
      961 tgtacaggga tatcaccacc agtacctcta cccccataca taccggagag caaatattcc
     1021 tgcgtcaaaa ctttatggat cttattgaaa cccgggcagt ggatgtcgta ggacccgatc
     1081 ctgctgacgt aggcggtatc aaagaactca agtggattgc agagtatgca gacctgcacg
     1141 gtattcttat ggctcctcac ggcacattca acggtgtgtt cggtatggct gcactgactc
     1201 atgtatgctc gacaatgccg cagaactatg tcgcctatga attcccggga gcaaatccag
     1261 gcttctggct ggatattatt gaaggtctgc caaataatta ccttcgtgat ggtcatgtga
     1321 aggtttggga tgctcctggc atcggcatta cctttaacgt caataaggct tccaagtact
     1381 taagtcccga agacaaagac ttcttcaggt aaatgtgtca aggggacgtt tcgtttgtca
     1441 cattttcttt gatgacaagg ggatgtttct tttgcaatcg gataaaataa gtaaaacatc
     1501 aaagccgacc ggggatattc ctgatcggct ttcatttctc cacggtaatc ccggctctgg
     1561 aatgatatgg taagaaccgg gattttaatt gaacatgcag cagtaccgga tgaaaatatg
     1621 atataattaa tgcaattatg gaatatatga ttaagcctca agacaaatgg tatcatggct
     1681 ctaatatagt atttataatt ctcggaaaaa gatagtacaa ttacacaatg ggggggaatc
     1741 ttgcaaaatc ccggtcctcc caaaggaaag gtatgatttg atatggcaat gacttcaaga
     1801 gagcgtttaa tcactgttat agaaggtaga ctaccggatc atgtccctgt agctccggat
     1861 acatccaata tgatccctgc ccgcttgacg ggaaagccct tctgggatct ctacctcttt
     1921 caggatccac ctatttggaa agcctacttg gattgtgtga agtattttgg tttcgattcc
     1981 ctgatggatg gttacgtatc catagatttt gatgatttac ccttggaaga tgatggttat
     2041 acctacgaag aggttattgt gtacaggtca cccgacagga tagttacaca aaagtacagg
     2101 gaggaaagag ggaaaaagat atgggctgat accgtggatg tgtattatat tgccgatccc
     2161 ccaaccatgg ggcttgaccc ccattcaata tccctaccgt ccatacccga tcattatgaa
     2221 ccaataggcg aaataaggca atggcccaag ggtgaggaac tattaaaata tgtaaaggaa
     2281 gaaatgggag accatggttt agtcggtgta agatgcggag taacaaagct tattgaaagc
     2341 gaatgggaga tatatgacta ttttgacaat cccgataagt attttgaaaa gcgagatcga
     2401 tacctggaac actatgtaaa aaagtttcat cggcttatgg agctggagtg caaacccgat
     2461 tttatttgta ccggtaattc cggaacactc atacaccaaa ctccacagat gtttagagac
     2521 ctggctcttc ctattgtcaa ggagataaca gccctatgca aagagtatgg cataccttct
     2581 cacgtacatt cctgcggccc ccagaaagag ctggttaaaa taatggccga ggaaacggac
     2641 cttaccataa ttgatcctct tgagatacct ccgatggggg attgtgatct aggcgagata
     2701 aaacgcctgt atggtgatcg cctggtatta aaaggcaaca tccatactac ggaagttatg
     2761 ctcaggggaa caacggctga tgtggcagag gctgcaaaaa gatgtataga ccaggcagca
     2821 gagggaggaa gatttatcct ctccactggt gaccaatgcg gcagggatac ccctgacgaa
     2881 aacatcttta cactgatcga ggtagccaag acctacggca ggtactgatg acaaggggac
     2941 gtttcgtttg gatgacaagg ggacgtttcg tttgtcacat ttctatggtg acaaggggac
     3001 gtttcgcctg tcatattttg aattaaacag aaaaaagggc gttaatttca gtgtaaacga
     3061 aaggatgccc tttttttgaa gtggcgtgtc tgaatatttc gaattaatcc cggatgcata
     3121 cctacttttc cagataagat gtaaagtatc catagaaact taagctatga agcctaatca
     3181 gtttccagac aaaggaagat aggatatagc agtatgaagg ggaatgaagt atcaaccagg
     3241 aagaacgaat gatcaggggg tctgaataat gagaaagcta caattagaac tgagtcaaga
     3301 cattattatt aataattgta gaagagagca agtaacacta tctgaatatg catgtaagag
     3361 tattagaagc gatagataca gagatgaaga gattccggat ttaaagaata taagaccatc
     3421 attcttccat gatactgata gaatcattca ttccagagca tataccagat atattgataa
     3481 aacacaagta ttctatctct ttgacaatga tcatattacc catagggtac tacatgtgca
     3541 gctggtatcg aaaataggaa ggacaattgg caggtgttta aggttgaacg aggacttgat
     3601 cgaagccata tcactggggc atgatcttgg acatgttccc tatggacata atggtgaaag
     3661 atatttaaat gagatatgtc aaaagagagg tattggatat tttatccata atgctcaaag
     3721 tgtcagggcc ctaatggaat tggaaaatgg gggtaaaggc ttgaatttga ccctgcaagt
     3781 actggacggg atactctgcc ataatggcga gtatttaaat caagcatatg aacccgataa
     3841 aaataaggat tgggataaat ttatggatga gtatacaaag tgctggcaaa tcaaaaatta
     3901 tgacaaacaa ataagaccca tgaccttgga agggtgtgtt atgcgcattt cggatgttat
     3961 cgcctatatt gggcgtgata tagaagatgc catagtggtt ggattgatta aacgtgaaga
     4021 tataccggat gaagttacca aggtattggg gaataataac aggcagataa tcaatactct
     4081 aataatcgat ctgctcaaca atagttatga caaaaattat ctgacattct ctgatgatgt
     4141 ttttaaggca ctagatacat tatttgattt caattacgaa catatatata gaaatcccca
     4201 aaaagcgaca caggatggta aaatagaaaa tatgttccat tatttatttg atgtatatct
     4261 ggatgatatt gaaaataata aagagtcttc gtccatttat aaatacttcc ttgatgaaaa
     4321 ggatgaggac tatagaaaga aaaatcgcaa tgaaagaata gtgatagatt tcattgcagg
     4381 tatgacagat gatttcttta ataatcaatt taaggaaaga atattcccga gcaaattcgg
     4441 aatgacaata taatctggaa tttatcctac taaaaactat ttatcgttga caatattata
     4501 caatatgtgt taaaatatcc atacaacaag gcaggcagac cggcagggtc tacctgcttt
     4561 tttagtaagg gagcttattg ttatgtatat agtactgata ttgattccta tagtggcagt
     4621 tggactaaag ttgttatcta tgcactttca gtatacggtg tttgtcaagt aagttgtcgt
     4681 ataaatttga tctagatata agtttccctt tagcatcact ccattatctg t
//
LOCUS       DASRNX010000228         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_527646,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000228 DASRNX010000000
VERSION     DASRNX010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527646"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFD89_08935"
     CDS             complement(<1..219)
                     /locus_tag="VFD89_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HZJ58340.1"
                     /translation="MYKKFLSPGKIGNLTLKNRTIFPPMGSGYVDNEYPKQQLIDYHV
                     RRVIGGCAMNIVEIAAVHPTTKSPTILGI"
     gene            617..1645
                     /locus_tag="VFD89_08940"
     CDS             617..1645
                     /locus_tag="VFD89_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58341.1"
                     /translation="MSSAFLHITKRNIDEYLLKYNIIACRLRNSRLKAKRVAIMELFM
                     ALLPLESVFLVNGPLSDKRGFVCFMIPNCSMETLSGILSYIGYCDTFFILDFGDIKVR
                     GDSYTGIERGTWKGRGFSVLELFRQCSETYDAQSPHNRPFVIIDGRGERRSIKGYRGD
                     GSAMGRRALPVEDCRCLVNLAIPSNIAHLVDPFAGAGGIVYAAQYINPDVNITTVDIE
                     PLLAPGLEMLGSKHFVEDAGQIQLPHRYDALVTEVPFDPQATPSVLKGLKNTLGYLTP
                     QGTASVMCSSQQFHMIEQLLVDAGMHIYCAHEINRKGTGVAIIAATQDPGVDKRAFSL
                     AQRVETVY"
     gene            1877..2239
                     /locus_tag="VFD89_08945"
     CDS             1877..2239
                     /locus_tag="VFD89_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZJ58342.1"
                     /translation="MSDKHGLVKIGIIGIVAGLCNGLFGSGGGIIVVPSMIHLLKVEE
                     HDAHATAIAVILPLSLVSVFIYFKSDYFDWNMVWKVCAGSVIGASIGAWLLPRIPVNM
                     LGRIFALFMIAAGLRMLF"
     gene            2240..2605
                     /locus_tag="VFD89_08950"
     CDS             2240..2605
                     /locus_tag="VFD89_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZJ58343.1"
                     /translation="MVLLLIGLVSGVVSGMGMGGGTILIPALIFLAQVPQHLAQSVNL
                     SVFFPASIVALWIHTKNKHVRYKLALKIITVGVLGAFIGSRIAVSMSTQILRKLFGFF
                     ILVMGVYELFRKPKGKDRE"
BASE COUNT          788 a          563 c          691 g          837 t
ORIGIN      
        1 tatccctaaa atcgtggggg atttcgttgt tggatgaacg gctgcaatct ctacaatatt
       61 catagcgcat ccgcctatta ctctccgaac atgataatct atcaattgct gtttagggta
      121 ttcattatca acataaccgg aacccatggg agggaaaata gtacggtttt tcagtgtcaa
      181 attgccgatt tttccggggg aaagaaactt cttatacata gatcaatctc ctcttcgcca
      241 aagttaatat tattattccg tatcagttct actttatgtg ctatcttttc tgtatccata
      301 gatttcgatg gtaggtttgt tggtatcctt gcctaagcaa atcatatcta gtacaatgga
      361 aatataatcc accattccat ggtagtaaga gtgggacccc agaacaggag agctaacaat
      421 ttccactaca caagccccaa ggggttttgt taaaggtaag gcatagtaaa tattaaaggg
      481 gctattaccg ggtctatacg attataaccc ataataaggc atatccataa agtccctgtt
      541 tggcatagtg aaatcctgag aatggaagag ttatttagag agaactaaaa ttataggaaa
      601 agggggtaat gggtgaatgt catccgcatt tttgcatata acaaagcgca atattgatga
      661 atacctttta aaatacaata taatagcctg tagactccgc aattcacgcc ttaaggctaa
      721 gcgggtggca atcatggaac tgttcatggc gttgttgccc ctggagagtg tatttttagt
      781 gaatggacct ctgagcgata agaggggctt tgtttgtttt atgatcccca actgcagcat
      841 ggaaacgtta tcgggcatcc tatcttatat tgggtattgt gatacgttct tcatcttaga
      901 ttttggggat atcaaggtca ggggtgatag ctatacgggg attgagcggg gtacctggaa
      961 aggtagggga ttttctgttc tggaactgtt taggcagtgc agtgaaacct atgatgcaca
     1021 atccccccat aaccggccct ttgtcattat agacgggcgg ggggaaaggc ggagtatcaa
     1081 aggatacagg ggtgacggca gtgcaatggg aaggcgtgcc ctgccggtgg aggattgtag
     1141 atgccttgtg aatctggcaa taccgagtaa tatagcccat ctggtggatc cctttgcagg
     1201 tgccggaggc atagtatacg ctgcccaata tataaatccc gatgtcaata tcaccacggt
     1261 ggatatcgaa cccttgctgg cgcccgggct ggaaatgctg ggatcgaaac attttgtgga
     1321 agatgcgggg cagatacagc tgccccatag atatgatgct ttggtaacgg aagtcccctt
     1381 cgatccccag gccactccct cggtgctaaa gggcctgaag aacaccttgg ggtacctcac
     1441 cccccaaggg accgcatccg tcatgtgttc cagtcagcaa tttcatatga ttgaacaatt
     1501 gctcgttgat gccgggatgc atatatactg tgcccatgaa ataaatcgga aggggactgg
     1561 ggttgccatt atcgcagcaa cacaagaccc aggtgtggat aaaagggctt tttcccttgc
     1621 ccagagggtt gaaacggtat actgatgggc caatgacttc attcatttgt tttgaaggtc
     1681 tgaattagga gttaggtact aaaagtgtca aaaaccatcg ctcaatatat tgggctacaa
     1741 tagggatgct acttgacgcc caacagctat taccagtata catataacca gcacaacctt
     1801 tggacttatc ttaagactag attatgcttt atgggaaaag ctaaaacaga tatcctattg
     1861 tagaaaaggt gataatatta gcgataagca tgggttagtg aaaattggta tcatcggtat
     1921 cgtagccggt ttgtgcaatg gactgtttgg atcaggcggc ggaataatag tagtaccctc
     1981 aatgatccac ctcttgaagg tagaagaaca tgatgctcat gccaccgcca ttgccgtgat
     2041 tttacccctg tcattagtaa gcgtttttat atactttaag agcgattatt ttgattggaa
     2101 tatggtgtgg aaggtatgtg cagggagtgt tattggggcg agcataggtg cttggctatt
     2161 gcctagaata ccggtgaata tgctgggcag aatatttgcc ctctttatga tagcagccgg
     2221 attaaggatg ctcttttaaa tggtattgct tttgattggc ctagtatccg gagtagtaag
     2281 cggaatgggt atgggtgggg gtaccatctt aatccccgcc ttaatatttt tggcacaagt
     2341 gccgcaacac ctggcccaaa gcgtaaatct aagcgttttc tttcctgcct ccattgttgc
     2401 actctggatt cacactaaaa ataaacacgt tagatacaag ttggccttga agataataac
     2461 agttggcgtc cttggggctt ttattggttc caggatcgca gtgtctatgt ctacccagat
     2521 tctccgtaaa ctatttggct tctttatctt ggttatgggg gtatatgagc tgtttagaaa
     2581 acccaaaggg aaggatagag aatagaatat attatccact gttaccttaa cctataaaga
     2641 aagttaaact atagtcatat aaaaccaccg ctatttactg aataacggct gtgctaattg
     2701 ttatatttcg ccggcattcg tttagtttcc agtttgggta aggcccaccg gctacgagag
     2761 taggggacac tttccgcacc gttatgccag ccgcatatgg tctatatggc ttcatcttag
     2821 cgtttgatga tactggtgtt atagcgtttt ggttcaaatt taacacgtct atccaattt
//
LOCUS       DASRNX010000229         3641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_260412,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000229 DASRNX010000000
VERSION     DASRNX010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260412"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFD89_08955"
     CDS             <1..746
                     /locus_tag="VFD89_08955"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZJ58344.1"
                     /translation="AKPRVFIPIFPGTASEYDLIRAFEKAGGRVETLVFRDLHPQEIR
                     ESIADISREIKRSQILVIPAGLSIGDELEKTGQMGTAILGAPQIQDAIKEHLEANDGL
                     ILGLGGGFETLLNLNLISDDSMDLILNPTGFHVSRIVETEILSNLSPWFMDAKVGDIH
                     TIAVSSREGQLVLDQKTTMELNERGQIATQFVPEEDSPISGIEGLTSRDGRVLGRLGH
                     PERLTSNVAVNVPHQNGYDIFKAGVSYYR"
     gene            complement(1073..2341)
                     /locus_tag="VFD89_08960"
     CDS             complement(1073..2341)
                     /locus_tag="VFD89_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZJ58345.1"
                     /translation="MELINEFIRKRIRFLIARPFLTAGIFTLVFSTLMTLVLGLKFYE
                     FAVITIIMAIAIELITYILVNEAKGKLESVAYIMQRIKNRDLNQSLDLNEFHGLESVS
                     MGFNNMMDELRSILGSLKTISLDLVQASEILNTNSEGINATMDDISATMDDIAHGSSE
                     QASEAERGVNLITNLSRQIHLVHQNTASVADDSENMRQLNSRGLEAVETLKTSNVRSA
                     RTAAEVSQFIRSFVEKSQSIGEFVSAINTIAEQTNLLALNAAIEAARAGEAGRGFAVV
                     ADEVRKLADNSKKSTEHVEDIMEGIMEEADTAFAMMDTMDEVVSDQTNAVDNTTDTFN
                     VIASNIENIIGRIDDISQSIAIMEKDKDDVTYAMQNISAVSEEAAAASQEIAAATQEQ
                     KNSIAEMVASSKTLNQLSLELRRHVEVYKV"
     gene            2529..>3641
                     /locus_tag="VFD89_08965"
     CDS             2529..>3641
                     /locus_tag="VFD89_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZJ58346.1"
                     /translation="MALKNRDMDMCNGPLFKKMIMFALPIMAMNVLQLMFNAADMVVV
                     GRFSGREALAAVGATGALINLIINLFMGLSVGTSVIVAQDYGADKHGDVSKSVHTSIT
                     ISVIGGLIVMIIGLVFCRPLLGMMGTPDDIINLSILYMKIYFLGMPASMVYNFGAAIL
                     RAVGDSRRPMYYLTISGTVNVIFNLFFVVVLKMGVAGVAWATVISQYLSMVLIMICLY
                     RSHGAIGFIPREMQIDGEKLKEIVRIGLPAGMQGLLFSISNVLIQSAVNSFGTTMIAA
                     NSASGNVEGIIGTTMNSYYNAAITFTGQNMGAKKYDRIDTIAKICTILIFSTWIVLGG
                     VILLIGRQMLGIYTTNPEVVELGMLRMKVMMVAYFTC"
BASE COUNT          981 a          775 c          852 g         1033 t
ORIGIN      
        1 tcgcaaaacc cagggtcttt attcctatat tcccgggaac cgcttccgaa tacgacctta
       61 taagggcctt tgaaaaggct gggggaaggg tagagacctt ggtattcagg gatttgcatc
      121 cccaagagat acgagagtcc atagccgata taagcaggga gataaaaagg tcacagatcc
      181 tagtaatacc ggctgggctc agcataggcg atgaactgga gaaaacggga cagatgggaa
      241 ccgctatttt gggtgctcca caaatccaag atgcaataaa ggagcatttg gaagcgaatg
      301 acgggcttat actggggctt ggcggcggat tcgaaacttt gcttaatttg aatctgatat
      361 ccgacgattc catggatttg attctcaacc caacgggttt tcatgtatct cgtatagtag
      421 aaaccgagat tttatccaac ttatccccct ggtttatgga cgcgaaagta ggggatatcc
      481 atacaatcgc tgtatccagc agggaaggcc aattggtgct ggatcaaaaa accaccatgg
      541 agctgaacga aagaggacag atagcaaccc aatttgtgcc ggaagaggac agccccatca
      601 gcggaataga gggtctcacc agcagagacg gcagggtatt gggaagactc ggacatccgg
      661 aacggctgac atctaatgtg gcggttaatg ttccccatca gaatggatat gatatattca
      721 aggcaggggt gtcctattac agataattga atgcgctatg cccgccggat ataaataact
      781 aactcaacca aggcatctgc ctgtcaatag tccactacct ttgctggaca gtaaaacaat
      841 ggactgacaa tatagcttaa tatatgttac gaaaaagacc tatcaataga tcaattcagt
      901 tttcgtaaca tataaatcaa ggaaaatgtt attcgatatc tgaaacataa tgtaaggtgt
      961 ttatcgtaaa acatatagat agcctaataa aaagaagggc gactgataat agtcgtcctt
     1021 cttttaaact gctaaacctt atagagttct ggttcctata gaccttcagc tgctagactt
     1081 tatagacctc cacgtgtctt cggagctcta aggatagttg attcaaggtc ttggaggaag
     1141 ccaccatctc ggctatagaa tttttttgct cttgggtggc ggcggcaatt tcttgactgg
     1201 ctgcagctgc ctcctctgat acagcggata tgttttgcat ggcatatgta acatcgtcct
     1261 tgtccttttc cattatggcg atggattggc ttatatcgtc tatgcggcct ataatgtttt
     1321 cgatattgga agcaataacg ttaaaggtgt cggttgtgtt atccacggca ttggtctggt
     1381 ccgataccac ctcatccatg gtgtccatca tcgcgaatgc ggtatcggcc tcttccatga
     1441 tcccttccat aatatcctcc acatgctcgg tggatttttt gctattatcg gctagctttc
     1501 gcacctcgtc agccaccacg gcgaagccac gacccgcttc ccctgctctg gctgcctcta
     1561 tagcggcgtt taaggccaag agattggtct gctctgcaat ggtgtttatg gcagaaacaa
     1621 attcgcctat gctctgggat ttctctacaa aggatcttat aaactgggat acctccgctg
     1681 ctgttcgggc gctccttacg ttagatgtct tgagggtttc gacggcctcc aggccccgag
     1741 aattcagctg ccgcatattc tctgaatcgt cagctacgct tgcagtattt tgatgtacca
     1801 aatgaatctg tcgggagagg ttggttatga gattgacccc tctttccgcc tcgctggcct
     1861 gttcagaaga accgtgtgcg atatcgtcca tagtagcgga tatatcatcc atggtggcat
     1921 ttatcccctc ggagttggtg ttcagtattt cagaagcctg taccaaatcc aaggagattg
     1981 tctttaaaga acccaggatg gacctgagtt cgtccatcat attattaaat cccatggaga
     2041 cggattccaa tccatggaac tcgttgagat ccaggctttg attcaaatcc ctgtttttta
     2101 tgcgttgcat tatgtatgcg actgattcca acttcccctt tgcctcattt actagtatat
     2161 aggttatcag ttcaattgcg atagccatga taatagtaat aacggcgaac tcatagaatt
     2221 ttagtcctaa gaccaaggtc atcagcgtgc taaataccaa ggtgaaaatc cctgcagtga
     2281 ggaaaggtct ggcgatgaga aatcttattc tcttacgtat gaactcattt atcaattcca
     2341 tttaagatcc tccccaaaaa ataataatat ttaccatatt atatcatgtt ttctttggac
     2401 tgcaaaggaa ttcgcgaaat ttgacgaaca caacacccat taagatatgg tatactatat
     2461 agggtatttt tcgtaataca aatagaagta atggggctca gccattcata aagggagtga
     2521 acacgtctat ggccctaaaa aatcgtgata tggatatgtg taatggaccg ttgtttaaga
     2581 agatgatcat gtttgccttg ccgattatgg caatgaacgt tctgcagctg atgttcaacg
     2641 cggcggatat ggtagtagtc ggccgctttt caggcagaga agcactggcc gcggtgggtg
     2701 caacaggcgc cctcataaat cttattataa acctgtttat gggactttca gtgggcacca
     2761 gcgtcatcgt ggcccaagat tacggcgccg ataagcacgg cgatgtcagt aaatccgtac
     2821 acacttcaat caccatcagt gtaattggcg ggttaattgt aatgataatc gggcttgtct
     2881 tttgcaggcc tctactgggt atgatgggta caccggatga tattataaac ctatcgatac
     2941 tttatatgaa aatatatttc ctcggaatgc ctgccagtat ggtctataat tttggtgcgg
     3001 caatcctgcg ggctgtgggg gacagccggc gtcccatgta ttatcttact atttccggta
     3061 cagtaaacgt catatttaac ctgttctttg ttgtagtttt gaagatgggg gttgccggtg
     3121 tagcatgggc tacggtgatt tcccaatacc tttccatggt gttaataatg atctgtctct
     3181 acaggagtca tggtgcaata ggcttcattc ctcgggagat gcaaatagac ggggaaaagt
     3241 taaaggaaat cgtgaggatt ggtttgcctg caggaatgca aggcttgttg ttttcaattt
     3301 ccaacgttct tatccaatcg gcggttaatt ccttcggcac tactatgata gcagccaact
     3361 ctgcatccgg aaatgttgaa gggattatcg gcactacgat gaactcctat tataatgcag
     3421 ccattacctt tacggggcag aacatgggcg caaagaaata tgacagaata gataccatag
     3481 caaagatatg cacaatcctt atattctcta cctggattgt tttaggtggg gtaatcctgc
     3541 ttatcggaag gcagatgctg ggtatataca ccaccaatcc ggaggttgtt gaacttggaa
     3601 tgctccgaat gaaggtcatg atggttgcgt actttacatg c
//
LOCUS       DASRNX010000230        30255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_364244,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000230 DASRNX010000000
VERSION     DASRNX010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 30255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30255
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364244"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..613)
                     /locus_tag="VFD89_08970"
     CDS             complement(119..613)
                     /locus_tag="VFD89_08970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HZJ58347.1"
                     /translation="MNFEEVFFMELGQILAISGGVTAVILAGIGSVLGVAMAGQSAAG
                     VVAEDPDKFGSVLLLQALPGTQGIYGMLIAFILMGKIGLLTTPLPVTTSQGWQLFAAC
                     MPIAIMGLLSAIYQGKTALAAIAMVAKRGETSGKGLTMTVMVETYAVLALLTSILMIN
                     GIQI"
     gene            complement(626..2593)
                     /locus_tag="VFD89_08975"
     CDS             complement(626..2593)
                     /locus_tag="VFD89_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HZJ58348.1"
                     /translation="MAVIEMKKIILVGMQGEKEGILKSIQSMGNVEIMEVQEHVDGQD
                     GEEISELIREEDSGAMEGTEAQLSKVESALGLLDRHKPLKKGMFDLKPQVEAHELLKI
                     LDSREEILSIADQCRSLEDELNELALRENRLRNTISQMEPWEALDIPLDSIEDTPTAA
                     VIAGTVDKGAAHRFLGAIEAMGDEPISVQELGETREDTCFFIVYHRSIEGDVQSILND
                     FAFGRSSFGGFTGTARRIIADSEKQLGEISKSRDDIADEITEFSNRRSDLELLYDALL
                     IELDRHRAVQNLERTRSTFILRGWVPAEQSEGLATTLRNEFKDIFIRLEDPAPDEDFP
                     VALDNPPIVSPFEMVTDLYSTPNPRELDPNAVMAPFYAIFFGLMMGDAGYGLIMALGT
                     YWFLKKMKPKDGTKKLVSVIFIGSIFTFIWGAIFGGWFGDAGELLGIPPIWFNPADEP
                     IKMLVVCFAMGVIHVFTGIGVKAYSNIREGRITDAIFDQGLWYVFYSGLILWLAGGMA
                     GLGPGASKVGKLMTIVGAIGLVLTQGREKDNIFAKFFAGLLSLYDVTGFLSDILSYSR
                     LFALALTTGVIGTVINQLGIMAGTSWYGWIIAAAVLIGGHAFNIAINVLGAFVHTSRL
                     QYIEFYGKFYEGGGRTFRPLGMRTKYRDIKR"
     gene            complement(2580..2909)
                     /locus_tag="VFD89_08980"
     CDS             complement(2580..2909)
                     /locus_tag="VFD89_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58349.1"
                     /translation="MSSTEVMNRIKEAEARAEEIRREAQQEARNILSKAQDDAEELAR
                     NLHRETVERGNKLMADTEAQAKQEIESLGEENVTLISKLRADAKANLDEAIFFVMGRI
                     VKDHGRN"
     gene            complement(2930..3559)
                     /gene="upp"
                     /locus_tag="VFD89_08985"
     CDS             complement(2930..3559)
                     /gene="upp"
                     /locus_tag="VFD89_08985"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HZJ58350.1"
                     /translation="MASVKIIDHPLIQHKLSLIRNKATGSKEFRELLEELALLMGYEV
                     THDLPLEEVEIETPIGPTTAKVISGKKVAIVPILRAGLGMVDGMLRLIPAAKVGHIGL
                     YRDPETLEPVEYYCKLPMDIGERDLIVVDPMLATGGSASAAITFLKKRGVRNIKFVCL
                     VAAPEGIDRIKKDHTDIDIFVAAVDSYLNEHGYIVPGLGDAGDRLFGTK"
     gene            3911..5143
                     /locus_tag="VFD89_08990"
     CDS             3911..5143
                     /locus_tag="VFD89_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HZJ58351.1"
                     /translation="MGLRHDAEKIIDSAIAAALPGTAVKKALEKEVFKGGRLVLVAIG
                     KASWSMAKAAWDILGDRISDGVVITKYDHARGDLPNIRIYEAGHPIPDANSFSATEKA
                     IELVEDLSPEDTVLLLISGGGSALFEKPLIPEHVLEDITEQLLSCGADIVEINTIRKR
                     LSAVKGGKFAKLCEPARVLAVVLSDIIGDPLDMIASGPAYPDTSTAEQAFDIIKKYGL
                     SVPESAMKLLAIEPPAALSNVTTKITGSVTQLCAAARDTSLALGYEPIVLTASLSCQA
                     REAGDFISSIAQYHGNSDKSLAFIAGGETVVRLKGSGKGGRNQELALAAAEGISGLDN
                     VAVFSIGSDGTDGPTDAAGGYVDHNTKKILTEKGMDIFDILENNDSYNALKACGGLII
                     TGPTGTNVNDLSVLLIKR"
     gene            complement(5156..5626)
                     /locus_tag="VFD89_08995"
     CDS             complement(5156..5626)
                     /locus_tag="VFD89_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HZJ58352.1"
                     /translation="MKSFLFVCTGNTCRSSMAEALFRRIIRSSGMDREEDLDISSAGV
                     SARKGERANSKAIKAMEELGIDLGDHSARQLTQEMIHRADLILTMTEGHRDFVRLMDP
                     GARYKTFTLKEYTDEGAAGAGDLDIEDPFGGPIEVYRSSAMEIKEALYRLMERL"
     gene            complement(5699..6763)
                     /locus_tag="VFD89_09000"
     CDS             complement(5699..6763)
                     /locus_tag="VFD89_09000"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZJ58353.1"
                     /translation="MQTKIYRIEDIKKDYRAIEEAAALIRKGQVVAFPTETVYGLGAN
                     ALDGQAVDRIFEAKGRPSDNPLIVHISKAEDIEPLVLEMPPGIHPLIEAFWPGPLTVI
                     LKKSKAVPHKTTAGLDTVALRMPNHPVALALIEGSKVPIAAPSANTSGRLSPTRAQHV
                     LDDLSGVIPMILDGISPCKVGLESTVLDMSGEIPTILRPGGITPEMLRGILGHVDLDE
                     TILKPTDNGHSPKSPGMKYKHYAPEAGVIIFKGSDLSAMAEKINEAHDEHVSRGLKVG
                     ILASDETIHRYTKGIIISMGSRRKPASIAARLYTSLREFDNAGVDIILAEWVDEREEG
                     LAIMNRMIRAAGFQVEDTSP"
     gene            complement(6900..7643)
                     /locus_tag="VFD89_09005"
     CDS             complement(6900..7643)
                     /locus_tag="VFD89_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZJ58354.1"
                     /translation="MANFLYSSIIGAGAGIVGTGLGGAMAFVLDKPGKKFLSILLSFS
                     SGLMVAVVCFDLLPEAFQIGGLFYGLLGTVSGVIAIIMCEEFINTRLSRVKSGYSSNK
                     YIQTGILVGIGIALHNLPEGLAIGSGFTARVSYGWELALIIGLHDIPEGVAMVTPMII
                     GGAGPLKAFLAAVAAGIPTGIGAMLGYLLGEISPLLICICLGFAGGAMLYITCNDLIP
                     ESRELHKGRTSTVGLIVGVVAGILVISAV"
     gene            complement(7729..8793)
                     /gene="prfA"
                     /locus_tag="VFD89_09010"
     CDS             complement(7729..8793)
                     /gene="prfA"
                     /locus_tag="VFD89_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZJ58355.1"
                     /translation="MIDKLDFIEERYEELNRSISDPEIINNQDEWKKLVIEHAEIEEI
                     VTIYREYRGVSEEIGEAEEMLQDKPDPEFRELLEQELEELSPKAEELEQRLKILLIPT
                     DPRDKKNVVVEIRGGAGGDEAALFGADLYRMYTRYAERHGWKSEVLSASFTDIGGVKE
                     LFFIIEGQGAFSRLKYESGVHRVQRIPTTESGGRIHTSTATVAVLPEAEEVDIDINPN
                     DLRIDVFRSSGHGGQSVNTTDSAVRITHLPTGLVVSCQDEKSQHKNRDKAMKILSARL
                     LDMAQQEEMAKYAEARKSQVGTGDRSERIRTYNFPQGRITDHRIGLTLYKLENFLDGD
                     LDEMIDALVTTAQAESLKNE"
     gene            complement(8919..9830)
                     /gene="prmC"
                     /locus_tag="VFD89_09015"
     CDS             complement(8919..9830)
                     /gene="prmC"
                     /locus_tag="VFD89_09015"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZJ58356.1"
                     /translation="MDLNVMEAMGKAQALLDRSGVTASQLEAGMLLAHLLDCPRTRLY
                     IDRDRDLTPAEIRAYFGLVDKRVRGTPIHYIIGHREFMGLDFCVEEGVLIPRPDTEVL
                     VEHIIERVKTMDNYVPSIIDIGTGSGAIAVSLAKYIDGSRVTAVDIDSGALGLAQRNA
                     DAYGLTDRIDFLWGDVFELFKGPGTGGQGPGFDVMVSNPPYIPTRDIEGLEPQVRDHE
                     PRIALDGGEDGLDFYRGLAGLAPNILNNGGLLAVEVGYDQACPVAHILKAAGCYGDIS
                     FVKDLSGYKRVVAAIFRSPAICPNLHP"
     gene            complement(9778..10752)
                     /locus_tag="VFD89_09020"
     CDS             complement(9778..10752)
                     /locus_tag="VFD89_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HZJ58357.1"
                     /translation="MKKSNIGGQAVLEGVMMKAPQRMAIAVRRSDGQIEVRSKSVTSI
                     KDRYPLLRLPVLRGIITFAETMVMGIKSLMDSAELYGEEEEYTPSRFETWISQKTGKK
                     AEDVMVFFALLIALGFAVLLFMVLPAMLAGFLGGIIKSNLVKSLIEGLIRLVAFVVYI
                     VAISRMKDIKRVFEYHGAEHKVIHCYEHEEDLTVENARKFTTLHPRCGTAFLLIVMVI
                     SILAFSFLEWNNIILRIAIKLVLLPFVAGISYEIIKWAGNSDSGLVGIVMYPGLMLQK
                     LTTKEPDDEQLEVAICSFLAAMDDPEEEGVGVGLECNGGYGESPGTAG"
     gene            complement(10932..11144)
                     /gene="rpmE"
                     /locus_tag="VFD89_09025"
     CDS             complement(10932..11144)
                     /gene="rpmE"
                     /locus_tag="VFD89_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZJ58358.1"
                     /translation="MKENIHPQYGEAVVRCACGETFITGSTKKELRVEICSKCHPFFT
                     GKQKLIDTGGRVERFKRRYGLSENEG"
     gene            complement(11588..12685)
                     /gene="ytvI"
                     /locus_tag="VFD89_09030"
     CDS             complement(11588..12685)
                     /gene="ytvI"
                     /locus_tag="VFD89_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HZJ58359.1"
                     /translation="MVRLTSKAQGILKWLGIAILIILGLFLFFTRLIKYVAPFLIAFI
                     VMISIEGLVTLLQRRLKFPRSPAVAVSLLLFVVVVGGILTFTFYRLTVEIWKLALELS
                     RMDFNPIIDYFESMFEKGQDLFLSLPRGLADTIEESLRLDAPRLSKIATEVSTRLMGI
                     VMGMVNFVKFLPDAVVFIIVTLISSYFMSRDRRQISEFLSKRFSPLWFDRMRSLRNDM
                     LLALVGFIKAQMILMAITFLELLVGFQIIGVKYAFFFALITAVVDALPVLGTGTILIP
                     TAIINFISGNVARGMGFLILYLIIFIIRQILEPKIVSQSLGLHPLVTLISMYAGLKFM
                     GVAGMLLGPIIAIIVRAFYKAGLFPTFRAPQ"
     gene            complement(12704..14434)
                     /gene="rho"
                     /locus_tag="VFD89_09035"
     CDS             complement(12704..14434)
                     /gene="rho"
                     /locus_tag="VFD89_09035"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZJ58360.1"
                     /translation="MDLNNLEKNTILQLREFGKELGVKSVTRHKKQKLIDLIKERLKE
                     MAVQQGEEQTIAEPAGGSTDEPIERTPRRESSEDTEKTEAKKETRTIEAGAEKDDKAT
                     GGAETPKPAFRGNIKRFPTPTRLSRTPHRTPAGGDWERGRENREATGERQEGDGQAKD
                     YDKKVAYARKYYNTSNPAIPELLSQDDTGDAQGVLETLPDGYGFLRSNNYLSGDHDVY
                     ISQSQIRRFNLKTGDLVQGKTRPAREGEKFQALLYVTAVNGENPEAANWRRPFEDLTP
                     IYPDKRITLEDPDNSKELATRLIDIISPIGKGQRGLIVSPPKAGKTILLKKIANSIAK
                     KYPDIYLIVLLIDERPEEVTDMQRSIDGDVVFSTFDELPEHHTRVAEMVLERAQRLVE
                     HGRDVVVLMDSITRLARAYNLTISATGRSLSGGLDPGALHKPKRFFGAARNIEEGGSL
                     TVIATALVDTGSRMDEVIYEEFKGTGNMEIHLDRRLSEKRIFPAVELSKSGTRREELL
                     LSQKELQGVYAMRKNLSEGRPDRVTEIIINDLMRTNSNDEFIDMLMDHFTRIDKDGYF
                     NDRDGYSGYR"
     gene            complement(15837..16289)
                     /locus_tag="VFD89_09040"
     CDS             complement(15837..16289)
                     /locus_tag="VFD89_09040"
                     /inference="COORDINATES: protein motif:HMM:NF020711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HZJ58361.1"
                     /translation="MGGGKKVILSIRGNQVNPDGEDNRIELITEGRLYKKGDAYYIEY
                     EESAISGLEGTKTLIMVEDRRISLERTGAHESRFVFEKGRKYINYYETPFGNLEMGIF
                     PTRIDSNMDENQGKLDVKYELDISGKYAGSNELMVSYQENGPQGLFKF"
     gene            complement(16378..17184)
                     /gene="murI"
                     /locus_tag="VFD89_09045"
     CDS             complement(16378..17184)
                     /gene="murI"
                     /locus_tag="VFD89_09045"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZJ58362.1"
                     /translation="MDNRPIGVFDSGLGGLTVVKELMKWLPQEDIVYFGDTARIPYGT
                     RSNETVIRYSQQCIRFLLSKDIKMIVIACNTVSSISLNMVTEMFDIPILGVIEPGARE
                     AVASTRNMRLGVIGTQGTIRSGAYEKAISRLDPRIRVFTNPCSLFVPIVEEGWSNSKI
                     ALLTAERYLDSMRCNDIDTLLLGCTHYPLLEDTIGKVMGPDVTLINPACGTALEVGRI
                     LKDRGLLSEDGTEAQYDFYVSDLGEKFEELGGRFLGREIKCARKIEIEMF"
     gene            complement(17337..18419)
                     /locus_tag="VFD89_09050"
     CDS             complement(17337..18419)
                     /locus_tag="VFD89_09050"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HZJ58363.1"
                     /translation="MQKLKIAVLFGGRSGEHEVSLSSASSIIENIDRDKYDIYLIGIT
                     KEGYWRYYDGPVEGIKTGRWEEDSKALVFPGDPAYRGFFLMEDPSRIFHVDVIFPVIH
                     GPYGEDGTLQGLFELAHIPYVGCDVLSSSTGMDKLTAKAIFSHADLPQGDYIGVYRHE
                     IGENPDEVVAIIEDSFLYPVFVKPANMGSSVGISKAGDRDELLAALDIAAKYDEKIIV
                     EEFIDGREIECSVLGNYDPKASVLGEIIPSEEFYDYYAKYEDGGESQRLIPAPLPTDK
                     NQEIRELAIKAYKALGCSGLARVDFFLKRQTGKVYLNEVNTMPGFTQISMYPELWEAS
                     GISYPDLIDRLVQLALDRFKMKHIYI"
     gene            complement(18700..18876)
                     /locus_tag="VFD89_09055"
     CDS             complement(18700..18876)
                     /locus_tag="VFD89_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HZJ58364.1"
                     /translation="MARSRRGLMSEELKTEIAKELGVHDIVQREGWGNVSSRNCGNIV
                     RKAIERAEKNLSGK"
     gene            complement(18974..20317)
                     /gene="ypeB"
                     /locus_tag="VFD89_09060"
     CDS             complement(18974..20317)
                     /gene="ypeB"
                     /locus_tag="VFD89_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HZJ58365.1"
                     /translation="MSRWIITGLLAVALAITGVWGYNQYLENQDHTIRTDNLYKKSFY
                     ELVGRVDNIESGLSKAMVSGDQGQSIAMLSDVWRQADSASTNLGQLPLSHIALNKTSK
                     YLSQLSDYCRYLTMKAGEGKPISLDEMKNLKELKNSSVRLNNELKDLESNVNKGMVTW
                     QEMRDMSNESLMDASQDLVGKQFTKIEETSIEYPTLIYDGPFSETLLRPKKLRVSGKK
                     IDYKEAERIAIDFLGRNRVESADKGAETQGEIETWGVNLKTRDGEGPYFVSVSKMGGK
                     VVSLICEGAVQGAGMDIGKAEDAAKKFLREKGYKDMIPTYEQHHGGMAVFNFAHKDGD
                     FIVYPDLIKVKISLDTGNPIGFEALNYLVAHRERDLKEPKLSPEDARKLINRNLEITS
                     ERLAVIPGKSGNEIECYEFKGKFDNENFIIYINSDTGREENILKVLNTDNGTMVI"
     gene            complement(20407..21135)
                     /gene="sleB"
                     /locus_tag="VFD89_09065"
     CDS             complement(20407..21135)
                     /gene="sleB"
                     /locus_tag="VFD89_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic enzyme"
                     /protein_id="HZJ58366.1"
                     /translation="MKTRNRLLVITILLTFFISAVMIVQYVQSGNDAEETLVYWGMYG
                     DKVFEVQQKLKQWGYYDGPIDGYYGAPTFEAVKKFQARNGLAVDGTVGNQTAAAMGIN
                     LGTKPPATGGGTSAPPSRGGDRGGGGNVYLLAQAIHGEARGEPYLGKVAVAAVVLNRV
                     ADPRFPNTIEGVIYQPGAFTAVSDGQINLAPDDESLRAARDALNGWDPTYGAVYYYNP
                     AKATSAWIWSRPVHLVIGKHHFAT"
     gene            complement(21247..21882)
                     /gene="spoIIR"
                     /locus_tag="VFD89_09070"
     CDS             complement(21247..21882)
                     /gene="spoIIR"
                     /locus_tag="VFD89_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HZJ58367.1"
                     /translation="MMYKRRGAFFLLMMIMPFLFTSGTYSNHGNNQACIRFHVIANSD
                     SPQDQAVKLMVRDRLLAEYGALLGQADSLGVSRHIIEERLRDIEASAQQETDRHLPGY
                     KVRASLGHFDFPTKAYGDMLLPAGNYEALRITIGEGKGANWWCIMFPPLCFVDITHGT
                     AKDDGDGGDETGDWETGEDISEQPSLGDDVHVVYRFKIAEWWEKAKEIIGY"
     gene            complement(21953..23014)
                     /locus_tag="VFD89_09075"
     CDS             complement(21953..23014)
                     /locus_tag="VFD89_09075"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZJ58368.1"
                     /translation="MLKLEWIIRLFSIFMAVLLSIILNPLNAFIFSELFFWVSLSYAL
                     LLLFKKYAYPWPLGSYYNLMIESLELMVLLYLHSASSSPMVVVLFISFLVRMALVYEN
                     KYSCPISLLTAGLFILVDFYTIDAGLKTGIWHRPIYWTVGFGFFIWTLWQARLLIEDM
                     ELKHRKMMESMGINETLIAELNESRDRLLETNDQLYTWANTDPLTNLYNVRYFNRFWD
                     NTFEQFQQFKQNNYPISLVMIDIQGHKIYSNVYGHIAGNVLLQDLAKILRECAHVEDI
                     VVRYSDYVFAVIIPGQQIGEAIQFYRNFKQRVTDYGRVHPGMRNIEISVGWSSCSDVF
                     HETKEELINRAINSMRLLD"
     gene            complement(23147..23914)
                     /locus_tag="VFD89_09080"
     CDS             complement(23147..23914)
                     /locus_tag="VFD89_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZJ58369.1"
                     /translation="MEKINVLVLAGDSGKREFGDGVTNKSLMDVYGKWMVEYVVDALR
                     DSEMVGKIAIIGPAETLKTRLAGRVDYFVQEETHLFENLKKGLSSFRDEEFVLVATSD
                     IPMIDGHIVSDFIEKCRVYNADLCYPIVNKTVNDAVYPGLKRTYVKLRDGIFTGGNII
                     GLNPKVVGSCAEFAKGFIAHRKNPWKLGRLLGFRFLLLLIVGRLSISRIEERFSELLK
                     IKARAIISPYPEIANDIDDPNEIGLAKQYLKEAKGKF"
     gene            24222..26030
                     /locus_tag="VFD89_09085"
     CDS             24222..26030
                     /locus_tag="VFD89_09085"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HZJ58370.1"
                     /translation="MIKKFLEDNILIADGAMGTYYSHLTGARSTVSELANIEDPRTVE
                     EIHRQYIEAGSKLIRTNTFSTNRYTLGKTRKEVSALLTGGYGIAKSSVGSRDVFVAAD
                     IGPIPEMIEGKAVEGAAIYDEYLFIADTFLKLGADVFIFETFSGTEYLEDLFEFIKSS
                     NPDSFILAQFALNAEGYTRKGISARRIQKQIKQIPSVDAYGFNCGVGPAHLHKIIREL
                     DFSGDLVSVLPNSGYPIIVNERTVYTENPEYFADRMMDIKDLGINIIGGCCGTTPLHI
                     DMLSKRLDSDSARIFATPKIVTEKPHIPKTPTNPFAHKLREDKFVIAVELDPPFSTDF
                     DKMVDGARELKESGVDILTIADSPMARSRIDSGMVAAKIKREVGIDTMPHLCCRDRNV
                     ISIKSWLLGAYIEGIRNLLIITGDPIPSPERNDIKSVFNMNSIRLIEMVAEMNREVFA
                     QEPFYLGGALNLNVLNKDVEVGRMKKKMKSGAKFFLTQPIFLDSTIEYLKGIRTNAQT
                     KILAGIIPLVSYRNAVFLNNEIPGIHIPDKFVKMFSPEMSRQEAEDVGIEIAAEIIGK
                     VKEHVDGIYLITPFNRTGMIARIIEKSLSPAERSPL"
     gene            26027..26824
                     /locus_tag="VFD89_09090"
     CDS             26027..26824
                     /locus_tag="VFD89_09090"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZJ58371.1"
                     /translation="MIIIGEKLNTSIDEVGKAVQDSDIDAIEQIALSQVKAGADYIDV
                     NCGTFVDREPELLKWMVTVLGECTHKPLCIDSPNPQAIGAALKVYGGHKPIINSITGQ
                     KRRYDSILPLVLEYGTSVIALCMDDDGIPGSADARVSVAERLVENLTNDGVDFEDIFI
                     DPLVQPMAMDQTNGLTTLRTIEGIKKSLPGIKTLCGLSNISFGLPDRSSINKAFLVLA
                     IHKGLDAAILDPLDRGIVSLSYAAKLMGGQDEYCLNYLESIKSLKEE"
     gene            complement(26869..27750)
                     /gene="ispE"
                     /locus_tag="VFD89_09095"
     CDS             complement(26869..27750)
                     /gene="ispE"
                     /locus_tag="VFD89_09095"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZJ58372.1"
                     /translation="MESICVRVHAKINLTLDVVGKRADGYHDVEMVMQSVNLWDEIVI
                     RVTDGKIELDSNVKGLPKDERNIAWRATELLKAEFGIPQGAGIFIRKNIPIAAGMGGG
                     SADCAGVLLGLNRLWNLNLGDGALKTLGKRLGADVPFCITGGTALAEGIGDILTPIAS
                     GTSIWLVIVRPDLRVSTGEVYGRLDLDYVEKRPNNKAMIEHLIAGRVSGVAENLVNVL
                     EGVTIPMHPQIAEIKSRLVDEGALGSLMSGSGPTVYGVFKDQSSARRAAKKMEKEYRH
                     TYVAETVQNGVLISRRP"
     gene            complement(27957..29528)
                     /locus_tag="VFD89_09100"
     CDS             complement(27957..29528)
                     /locus_tag="VFD89_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOCS domain-containing protein"
                     /protein_id="HZJ58373.1"
                     /translation="MAINYEKGLIKIDQLVGEELSQHLVEGELIVHEEEPEIAKILDV
                     WAVVYGTGKEVVQDKVMVEGILRYNLLYIPVGDSKSIAQAESETGFVEYVDLEGAKPR
                     MTADISFELEHLDYDILSSRRLGVKAVLNIDCRVRELLSLEVLKDFPDEDGIQALREP
                     MELVFTGGDGSGQTIIREDVELPDDMPSVIRVLRKDTKVKILETRVTDNRILAQGEID
                     LKLLYQSGEEQDPIQLLQNQIPFNHFVEVQGAYQGMDCDVKVEAQELDVSLRQDIIGD
                     TRILSVDMILLMEGRVYESHEEDLIIDAYSPGTALNLVKQKIMLTRPMGKGQAQSIIR
                     ENIELGENLPPAGRILYAEARPLVADYRIGQGQVVVDGVLTGILLYKPEDDGLGISSI
                     KADIPFSQTLEIEGINEDMECLCEAVVQYLSHTLISPNEVELKITMLVTGEATEEIEK
                     EVLLEIEEIEAEGEADSGIYIYFVQPGDNLWSVAKRYNTTINTILRYNEIADEGVLEA
                     GSKIMIFKKLDASIV"
     gene            complement(29719..29979)
                     /locus_tag="VFD89_09105"
     CDS             complement(29719..29979)
                     /locus_tag="VFD89_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HZJ58374.1"
                     /translation="MVINIDALDKVRETLESNIGERVKLKANRGRRRTYVNEGIINDI
                     YPSVFTVLVDIDGQSTRTLCYTYSDVLTSNVELVVCKNNQRI"
BASE COUNT         7576 a         8016 c         6295 g         8368 t
ORIGIN      
        1 ataatacggg cagcttcagc ccgggcatcg tctaaaatcc ttttgctaat tctatcgatt
       61 ccgttcatgg ctcacactcc catgcaagag ctatatttta taacataatc cagtgtaatc
      121 aaatttgaat cccgttgatc atcaatatag aagtcaagag tgcaagaacg gcataggttt
      181 caaccataac cgtcatagta agacctttac ccgaagtctc gcccctttta gctaccattg
      241 caattgcggc aagggctgtc ttaccctggt agatagccga caggagtccc ataatggcaa
      301 tgggcataca tgccgcaaag agctgccatc cctgtgatgt ggtgacgggc aggggtgtgg
      361 ttaacagtcc gattttgccc attaagataa aggctatgag catcccatag atcccctggg
      421 tcccgggcag tgcttggagc aataatacgg atccgaattt atcggggtcc tctgctacga
      481 ctcccgctgc gctttgtccc gccatggcca cacccaacac cgacccgata cccgccagaa
      541 ttaccgccgt tacgccaccg ctgattgcta agatctgacc taattccata aaaaatacct
      601 cctcaaaatt gatgattgat tttgcttatc ttttaatatc cctgtacttc gtcctcatgc
      661 ccaggggcct aaatgttctg cccccgccct cgtagaactt tccgtaaaac tcgatgtact
      721 ggaggcggct ggtatggacg aaggcaccca ggacgtttat ggctatatta aaggcatgcc
      781 cgcctatcaa gacagcagcc gctattatcc agccgtacca cgatgtaccc gccatgatgc
      841 ccagctgatt tattaccgtc cctataaccc cggtagtaag ggccagggca aacagtctgg
      901 aataggagag gatatcgctc aaaaatcccg tcacatcgta caggcttaga aggcctgcga
      961 agaatttggc gaaaatattg tccttttccc ttccctgggt cagcacaaga ccgatggctc
     1021 ctactatggt catgagcttt ccgaccttgg aggctccagg ccccaggccc gccattccgc
     1081 cggccagcca cagtataagg cctgaataga atacatacca tagaccctga tcaaatattg
     1141 cgtccgtgat gcgtccttcc cggatattgg aataggcctt tacccctatc cccgtgaaga
     1201 catggatcac gcccatggca aaacagacta ccaacatctt aatgggctca tccgccgggt
     1261 taaaccagat ggggggtatc cccaacaatt ccccggcatc gccgaaccat cccccaaata
     1321 tggcgcccca tataaaggta aagatacttc cgataaatat caccgagacc agctttttgg
     1381 tgccgtcctt gggcttcatc ttctttagga accaataggt cccaagggcc ataataaggc
     1441 catatcccgc atcccccatc atcaggccga agaatatagc gtagaagggg gccattacgg
     1501 cattggggtc caattccctg ggattcgggg tactataaag gtccgtcacc atctcaaagg
     1561 gcgagacgat aggagggtta tccagggcca cggggaaatc ctcatccggg gccggatctt
     1621 caagtcttat aaagatatcc ttgaactcat tccttagggt tgtcgccaga ccttccgatt
     1681 gctccgccgg cacccatccc cttaatataa aggtgctgcg ggtccgctcc aagttctgta
     1741 cagctctatg cctgtcaagc tctataagga gagcatcata gagaagctcc aagtccgatc
     1801 tgcggttaga aaattcagtt atttcgtcgg caatgtcgtc cctggacttt gaaatttccc
     1861 cgagttgttt ttcggaatcg gctattatcc ttcgggccgt cccggtgaat ccaccgaagc
     1921 ttgacctgcc gaaggcaaaa tcgttgagga tggattgaac atcaccctct atggatctgt
     1981 ggtaaacaat aaaaaaacag gtatcctccc tggtttcacc cagttcctgc acggatatag
     2041 gctcatcacc catagcttct atagcaccta aaaatctgtg agccgcaccc ttgtccaccg
     2101 tgccggcgat aaccgcagca gtcggagtat cctctatgga atccaagggg atatccaagg
     2161 cctcccaggg ctccatctgg cttatggtat ttctcagcct gttctccctg agggcgagtt
     2221 cgttaagttc atcctccagg ctcctgcact ggtcagcgat gcttaggatt tcttctctgg
     2281 aatccagaat ctttagcagt tcatgtgctt caacctgggg ctttaaatca aacatccctt
     2341 tttttagggg cttatgcctg tcgagaagtc ccaaggcgga ttccaccttg gacagctggg
     2401 cttcggtccc ttccatagcg cccgaatcct cttcccggat cagctccgaa atttcttcgc
     2461 cgtcctgtcc gtcaacatgc tcttggactt ccattatctc cacatttccc atggattgaa
     2521 tggattttag tattccctct ttctcaccct gcattccgac caggatgatc tttttcatct
     2581 caattacggc catggtcttt cacgatcctt cccattacaa aaaaaatcgc ctcatccaga
     2641 ttggccttgg catccgccct tagcttggaa atcagggtga cattttcttc ccccagggat
     2701 tctatctctt gtttggcctg ggcctccgta tcagccatca gcttattccc ccgctcaacg
     2761 gtttccctgt ggaggttacg ggccagttcc tctgcgtcat cttgggcttt gcttaaaata
     2821 ttacgggcct cttgttgggc ttcccttcta atctcctctg cccgggcttc cgcctccttt
     2881 attctgttca tgacttccgt agaagacatt ttaccaccgc ctaaacatat catttggtac
     2941 caaaaagtct gtcaccggcg tcccccaaac caggtacaat atatccatgc tcatttagat
     3001 aactgtccac cgctgccaca aagatatcaa tatccgtatg gtccttcttt atcctatcaa
     3061 taccctccgg cgcagcgacc aaacatacaa actttatatt acgtacgccc cttttcttaa
     3121 gaaaggttat agccgcggag gccgatcccc cggtagccag catggggtct acgacgatta
     3181 aatccctttc ccctatgtcc atggggagct tacaatagta ttccacgggc tctaaggtct
     3241 ccggatcccg atatagacct atgtgcccta ccttggccgc tggaatcagt ctcagcatcc
     3301 cgtccaccat tcccagcccc gctcttaaga tgggcacgat ggctaccttc tttcccgaga
     3361 tgaccttggc cgtagtaggc cctatgggcg tctctatttc aacttcttcc aggggcagat
     3421 catgggtaac ctcgtaaccc attaggaggg ccaactcctc caacaactcc cgaaattctt
     3481 tggaacctgt ggccttgttt cgaattaaac tcagtttatg ctgaatcaga ggatgatcaa
     3541 tgattttcac actagccacc ttctattcct ccttttccta tgtaaatata atagccggca
     3601 agtaatgagt ccctaaaatg ccattatata ttatatactt ttaaactgct cagtgcaact
     3661 ttatttatac ttcgtcgcat gttttgcggt atgcggcaat tggtaaaaac tgcggcctgt
     3721 taatgcactc cagcggggat tctgttataa ttgttttgta ccactgttga tcccttgtcc
     3781 ggttatacat aggttaggga atggtaccgc gctatcgtcc atgcaaatta cgctgcgcac
     3841 cattgaagga tttaaaagga cttaatccct tgatcgaatt agcttatagc tctggaaagg
     3901 agtgatccca ttgggtttga gacatgatgc tgaaaaaatt atcgattctg ccatagccgc
     3961 ggcgcttccc ggcacggcgg ttaaaaaagc gctggagaag gaggtgttca agggcggcag
     4021 gttagtattg gtcgccattg gcaaggcctc ctggtccatg gccaaggcgg cgtgggatat
     4081 actgggggat aggattagcg acggtgtcgt gatcacaaaa tatgatcatg cccgggggga
     4141 tttacccaac attcgaatct atgaggcagg ccatccgata ccggatgcca actccttctc
     4201 cgccacggag aaggccatag agttggtgga agatctttca ccagaagaca cggttctgct
     4261 tctcatttca ggcgggggtt ctgcgctctt tgaaaagccc ctgatcccgg agcatgtgct
     4321 ggaggacatt accgaacaac tcctgtcatg cggcgccgat atcgttgaaa taaacacgat
     4381 tcgaaaacgc ctgtcagccg ttaagggggg aaagttcgcc aagctgtgtg aacctgccag
     4441 ggtgctggct gtggtcttat ccgacattat cggggatccc ttggatatga tagcttccgg
     4501 gccggcatat ccggacacct ctaccgccga acaggccttt gatatcataa aaaagtacgg
     4561 gctttcggtg cccgaatccg ccatgaaatt attggccatt gaacctcccg ctgccttatc
     4621 caacgttacc acgaagatca ccggcagcgt tacacagctt tgtgccgctg cccgagatac
     4681 gagcctggcc cttggctatg agcccatagt cctcaccgcc agcctgtctt gtcaagccag
     4741 ggaggccggg gatttcatat cctccatcgc acaatatcac ggaaactccg acaaatccct
     4801 cgcctttatc gccggcggcg aaaccgtagt gcgcctaaag ggtagcggca aggggggacg
     4861 caaccaagaa ttggctctgg ctgcggcaga gggcatatcg ggtctcgata acgtggcggt
     4921 cttttccata ggctcggacg ggaccgacgg tcctaccgat gcggcaggcg gatatgtgga
     4981 tcataatacc aagaaaatcc tcacagaaaa aggcatggat atcttcgata tcttggaaaa
     5041 taacgactct tataatgccc ttaaggcatg tggcggactt ataataacag gccctacggg
     5101 caccaacgta aacgatttgt cggtgctcct aataaaaaga taggcgggat gtcccctata
     5161 atctctccat gagcctgtat aaggcctctt tgatttccat ggcgctactc ctgtaaacct
     5221 caatcggtcc gccgaaggga tcttcgatat ccaaatctcc ggccccggct gcaccttcgt
     5281 cggtatactc cttcagggta aaggtcttat acctagcgcc gggatccata agccttacaa
     5341 aatccctgtg tccctcggtc atggtaagaa ttaagtccgc acgatggatc atctcttggg
     5401 tcagttggcg ggcgctatgg tcgcctaaat caattcccaa ctcctccatg gctttgatgg
     5461 cctttgaatt ggctctttcg cccttcctgg ccgatacgcc ggcagaggat atatcaagat
     5521 cttcctccct atccataccg gagctcctta tgatgcgccg aaacagcgcc tctgccatac
     5581 tgctcctgca tgtattgccg gtacaaacaa atagaaagct cttcattgaa acttcaactc
     5641 cttgctggac gggcttttta tttcccgact ataaaaacga gaacgacctt cccatcctct
     5701 atggggatgt atcctccacc tgaaatcccg ccgccctaat catccggttc atgatggcaa
     5761 gaccctcttc cctttcgtcc acccattcag ctaatattat atccacacct gcattatcga
     5821 attcccttag ggaagtgtag agcctggcag caatggaggc gggctttctt cggctcccca
     5881 tgcttattat tatgcccttg gtatagcggt gtatagtctc gtctgatgct aatataccca
     5941 ctttcaggcc gcggctaacg tgttcgtcat gggcctcgtt gattttttct gccatagcgc
     6001 tcaaatcgga ccctttaaat attataaccc cggcttcggg ggcatagtgt ttatacttca
     6061 taccggggga tttggggctg tgcccgttat cggtgggttt taagatggtc tcgtccaaat
     6121 cgacatgacc taagatcccc ctgagcattt cgggcgtaat acccccgggc cttaaaatgg
     6181 tggggatttc accgctcata tcaagtacag tagattcaag gcccaccttg cagggactaa
     6241 ttccgtccaa tatcatgggg atgacaccgg acaggtcatc caatacatgc tgggctctgg
     6301 tggggctgag cctcccggag gtgttggcgc tgggggcagc gatgggaacc ttcgacccct
     6361 caataagggc cagagctacg ggatggtttg gcatgcgaag ggcaacggta tcaaggcccg
     6421 ccgttgtctt atggggtacc gccttcgatt tttttaatat gacggtaagt gggcccggcc
     6481 aaaatgcctc aatcaaggga tggatccccg gcggcatctc cagaaccagg ggttctatat
     6541 cctctgcctt cgatatgtga actatcaatg ggttatcgct gggtcttccc ttggcctcaa
     6601 atatcctgtc cacggcttgt ccatccaagg cgttggcccc aagaccgtat accgtctcgg
     6661 taggaaaggc aaccacttga cccttgcgta ttaaggcagc cgcctcttct atggccctat
     6721 aatccttttt tatatcctct atcctatata tcttggtctg caaatcaatc tttctcctta
     6781 aataaaacgt agataaacac aaggaacaca acagaacaca aggggacggt ccttctgtgt
     6841 tcgtacttcc aaactgcaca atattctcta acacaacagg accgtccccc tgtgtttttt
     6901 tagacggcag agattactag gatgcctgcc acgaccccga cgatgagccc gacggtggac
     6961 gtcctcccct tatgtaattc cctggattcc ggtatcaggt cgttacacgt tatgtacagc
     7021 attgctcccc ctgcaaaacc taagcatata catatgagaa ggggggagat ctcacccagc
     7081 aaatagccta acatcgcccc tattcccgta ggaattcctg cagctaccgc cgccagaaac
     7141 gccttcaggg gccccgcccc acctattatc ataggggtga ccatcgccac cccttcgggt
     7201 atatcgtgaa ggcctatgat gagggccaac tcccagccat atgataccct ggcggtaaat
     7261 cccgaaccta tggccaagcc ctccggcaga ttatgaaggg ctatgccgat tcccaccaat
     7321 atccccgtct gtatatactt attggacgaa tatccacttt ttaccctact cagacgggta
     7381 tttataaatt cctcgcacat tattatagct atcacgccgg agaccgtccc taaaagaccg
     7441 tagaacaggc ccccaatctg aaaggcctct ggcaacagat caaagcaaac caccgctacc
     7501 atgagccccg acgaaaagct gagcagtatg ctcaaaaact tcttccccgg cttatccaat
     7561 acaaaggcca ttgcgccgcc cagtcccgtc ccaacgatac ccgcaccggc accaatgata
     7621 gaactatata gaaagtttgc caatatccat tcccccaaat aaacaatata tagtagattg
     7681 tattcaggga aacggacatc tatgatttca gatgcctata cagcctctct attcgttttt
     7741 caggctctcc gcctgggcag tggttacaag ggcgtcgatc atctcgtcga gatccccgtc
     7801 tagaaagttt tccagcttgt atagggtaag ccctatccta tggtcggtta tcctaccctg
     7861 gggaaaattg taggtgcgga tccgctcgct cctgtcgccg gtccccacct ggctctttct
     7921 ggcctccgcg tattttgcca tctcctcctg ctgagccata tccaagagcc tggcacttag
     7981 gatcttcata gctttgtccc tgttcttatg ttgggatttt tcgtcctggc aggataccac
     8041 aagtcccgta ggcagatggg ttatcctgac ggcggagtcc gtagtattta cgctctgtcc
     8101 cccatgaccg gaggatctga atacatcaat tctcaaatcg tttgggttta tatcaatatc
     8161 cacttcctcc gcctccggga gaaccgccac cgtggccgtg gaggtatgga tccttccccc
     8221 ggattcggtg gtggggatcc tctgaaccct gtgtacaccg ctctcatact ttaagcgact
     8281 aaaggcgccc tgcccttcta tgataaaaaa taattcctta acgccgccta tatccgtaaa
     8341 gctggcgctt aagacctcgc tcttccagcc gtgacgttct gcatagcggg tgtacatcct
     8401 gtatagatcg gcaccaaaaa gggctgcctc atcgccacct gcgccccccc gaatttcaac
     8461 tacgacattt ttcttgtccc tggggtctgt gggtatgagg agtatcttaa gcctctgttc
     8521 caattcctcc gctttagggg acaattcctc cagctcctgt tccagcagct cccgaaattc
     8581 cgggtccggc ttatcctgga gcatctcctc ggcttcccct atttcctccg acacccccct
     8641 gtactccctg tatatggtaa ctatctcctc tatttcggca tgctcaatga ccagcttttt
     8701 ccactcgtct tgattgttga ttatctcggg atcggatatg gatcggttta actcttcata
     8761 cctttcctca ataaaatcaa gtttatctat catagtaatg ctccttttga ctttgactta
     8821 cgagaaatcc cctgtctttt tatctatcta gcctgaacct atggggcaca gatgattctc
     8881 tggcaacctt taatcaaccc atggaacctt acccgcttct atgggtgcag gttaggacat
     8941 atcgcaggcg atctgaaaat ggctgccact acccgcttat acccgctcaa atccttgaca
     9001 aaactgatat ccccatagca tcctgcagcc ttaagaatat gggccacagg acatgcctga
     9061 tcatacccta cctctacagc caagagcccc ccgttattta gaatgttcgg ggcaaggccc
     9121 gccaaacccc tgtaaaaatc cagcccgtcc tctcccccat ccagagctat tctgggctca
     9181 tgatccctga cttggggctc caagccttct atgtcccggg taggaatata ggggggattg
     9241 gataccataa catcaaaccc cggcccctgt ccaccggttc cgggtccttt aaaaagctca
     9301 aatacatcgc cccatagaaa gtctatccta tccgttaacc cgtaagcgtc ggcatttctt
     9361 tgggccagcc ccagggcgcc gctgtcaata tccaccgccg tcacccgaga accgtctata
     9421 tactttgcca ggctgacggc tatggccccg cttccggttc caatatctat tatgctgggg
     9481 acgtagttat ccatggtctt aacccgctca ataatatgct ctaccaaaac ctctgtatcc
     9541 ggccgtggaa ttagaacgcc ttcttctaca caaaaatcca gtcccataaa ttccctgtgg
     9601 cctattatat agtggatggg ggtgcccctg acccttttat ctaccaaacc gaagtatgcc
     9661 cggatctctg ccggggttaa gtccctatcc ctgtcaatat acaggcgggt cctcgggcaa
     9721 tccagcagat gagccaagag cattccggcc tccaattggg atgcggtgac acccgaccta
     9781 tccagcagtg cctgggcttt ccccatagcc tccattacat tcaagtccaa cacctacccc
     9841 ctcttcctcg gggtcatcca tagccgccaa gaatgaacat atggcaactt ccagctgctc
     9901 atcatccggt tccttggtgg tcagcttttg cagcatcaat cccggataca tgactatccc
     9961 tacaaggccg gagtcgctgt ttcctgccca cttgatgatc tcataggaaa tccccgccac
    10021 aaagggcaac aataccaact ttatggcgat cctcaatata atattgttcc actccaagaa
    10081 ggaaaaggca aggatgctga tcaccatgac tatgagaaga aacgctgtac cgcacctggg
    10141 atggagggtg gtgaattttc gtgcattctc taccgtcaaa tcctcctcat gctcatagca
    10201 gtggatgacc ttatgctctg ccccgtggta ttcaaatacc cttttaatat ccttcatccg
    10261 gcttatggcc acgatatata ctacaaaagc aaccaggcgt attaacccct ctataaggct
    10321 cttgaccaaa ttgctcttta ttataccgcc caaaaatccg gccagcatcg cgggtaacac
    10381 cataaacaac aagacagcaa agcccagggc tatcaagagt gcaaagaaca ccataacatc
    10441 ctcagccttc ttcccggtct tctgggatat ccatgtctca aaccgagacg gcgtatactc
    10501 ctcctcttcc ccatagagtt cggcggaatc catcagggat tttattccca tgaccatggt
    10561 ctccgcaaag gtgattattc ccctcaggac aggaagtctg agcaggggat atctatcctt
    10621 tatagaagtg acactcttgc tccttacttc tatctgtcca tcgcttcgcc ttacagctat
    10681 tgccattctt tggggagcct tcatcattac cccctctaat accgcctgac ccccgatatt
    10741 gctctttttc atataaacac cttatttctc tatatttccg gacccttgta ttaatttaaa
    10801 gtattgtgaa agtttattat ccggaatgag ttattcataa gcggatgact ccattgaccc
    10861 gttgcaatta tcccgccgta taacaaaaaa tacagaccag ggaaatcccc aatctgtatt
    10921 cggcttttgc attatccctc gttttcactc aagccgtatc ttctcttaaa tctctcgaca
    10981 cgaccgccgg tatcaatcaa tttttgtttt ccggtgaaga aaggatggca cttggagcat
    11041 atttcaaccc taagctcctt tttggtggaa cctgtaataa aggtctcacc gcaggcgcat
    11101 cggaccactg cttcgccgta ttggggatgt atattttcct tcatggattt cacctctttc
    11161 caaaggattc gaattaattt catcgtattg taaaaatatc aatggtaatg ccctacgata
    11221 tccgaaacat aatttaacgt attctgccgg tcaaatatat gtcggtaaag gctgctaagc
    11281 gctgcctcca tactgcttat aggtggtcta ttacctctgt ttggtaatag taaggcgaac
    11341 cggcatggaa gattaatttg tagcatatat cgcaaaacat ataacaatca aataacttgt
    11401 ttagtataac ataaatgatc gatataatgc aagaccggat ttttatcgtc cgggcacata
    11461 ttctgacccg ttatcccggc aatcttccag ggttttagcc ggctgatggc cggcgaatgt
    11521 tcatcaagct accccatatc cggcttgatt tcccccatac aagtcaaaat tgtcatacat
    11581 gtcctaacta ttggggcgcc ctaaatgtgg gaaacagacc tgccttataa aacgccctga
    11641 cgataattgc tattatggga cccaacaaca ttcccgcaac ccccatgaac ttcagacctg
    11701 cgtacatgct gataagggta accaacggat gcaatcccag ggactggctg acaatcttag
    11761 gctctaggat ctgtctgatt ataaatataa tgaggtagag aatcaaaaat cccatgcctc
    11821 tggccacatt gccggagata aagtttataa tcgcggtggg gatgaggatc gtaccggtcc
    11881 ccagcaccgg cagtgcatct acaactgccg tgatcagggc aaaaaagaac gcatatttta
    11941 cccctattat ctgaaagcct acaagcaatt ccaaaaaggt tatggccatc aagatcatct
    12001 gtgccttgat aaagcccacc aacgccagga gcatatcgtt cctgaggctc ctcatcctgt
    12061 caaaccacag gggggaaaac cgctttgaaa gaaattcgga tatttgtctt cgatcccggc
    12121 tcataaaata ggatgatatc aaggtgacta ttataaagac cacggcatcg ggcaaaaatt
    12181 ttacgaagtt taccatgccc atgactatcc ccataagcct ggtggaaacc tctgtggcta
    12241 ttttggacag cctgggggca tccagtctga ggctttcttc aatggtatcc gccagacccc
    12301 tgggcaggct caaaaacaaa tcctgcccct tttcaaacat ggactcaaag taatctatta
    12361 tggggttaaa atccatcctg gataattcaa gggccagctt ccaaatctca acggttaacc
    12421 tataaaaggt gaacgtcaaa atgcccccca ctaccaccac aaatagcaat aatgaaacgg
    12481 ctacggcggg gctcctcgga aacttgagcc tcctctgcag gagggttacc aaaccctcta
    12541 tggatatcat tacgataaaa gctatcaaaa aaggtgctac atacttgata agccgagtaa
    12601 agaacaggaa taatcctaag attattagaa ttgctatacc caaccacttt aatatccctt
    12661 gcgctttcga tgttagtctg accatacccc tattgctcct atcctatctg tatccggaat
    12721 acccgtccct gtcgttaaag tatccgtcct tgtcgatcct tgtgaagtga tccatcagca
    12781 tatctataaa ttcatcgttg gaattggtgc gcataagatc gttgattatt atctcggtca
    12841 ccctgtcagg cctgccctcg ctcaagtttt tgcgcattgc atagacgccc tgcaattctt
    12901 tttggctgag gagcagctct tcccttctgg ttcccgattt gctcagctca actgcgggga
    12961 atatcctctt ctccgacaga cggcgatcca aatggatttc catgttgccc gtccccttaa
    13021 attcctcata gataacctcg tccattctgc tcccggtatc cacaagagca gtagcaatga
    13081 ccgtgaggct tccgccctct tcaatatttc tggccgcacc gaaaaacctc ttgggtttat
    13141 ggagcgcacc cggatccaat ccgccggata agcttctgcc ggtagcggag atggttaaat
    13201 tatatgccct ggccagacgg gttatgctgt ccataagaac cactacatcg cggccgtgct
    13261 ctacaagcct ttgggccctt tctagaacca tctccgcgac cctagtatgg tgctcaggca
    13321 attcatcgaa ggtggaaaac accacgtcgc cgtcgatgga acgctgcata tccgtaacct
    13381 cttccggccg ctcatctatc aggaggacta ttaaatatat atccggatat ttcttagcga
    13441 tgctgttggc gatctttttt aataggatgg tcttaccggc cttgggcggc gagactataa
    13501 ggcccctctg ccccttaccg atgggggata tgatatctat aagcctggtg gcgagttcct
    13561 ttgagttgtc cggatcctcc agggtaatgc gtttgtcggg atatatgggg gtgaggtctt
    13621 cgaagggtcg tctccagttt gccgcctccg ggttctcccc gtttacagcg gttacataga
    13681 gaagtgcctg aaatttctcg ccttctctgg caggccgggt cttgccttgt accagatccc
    13741 ctgtcttaag gttgaatctt cttatctggg attgagagat ataaacgtcg tgatccccgg
    13801 acagatagtt gttggacctg aggaacccat agccgtcggg caacgtttcc agcactcctt
    13861 gtgcatcccc cgtatcgtcc tggctcagga gctccggtat agcagggttg gatgtgttgt
    13921 aatatttcct ggcataggct accttcttgt cataatcctt agcctgccca tccccttcct
    13981 gtctctcccc ggttgcctct ctgttttctc ttcctctttc ccaatcccct ccggccggtg
    14041 tcctgtgtgg ggttctggac aggcgtgtgg gggtcggaaa tcgcttgatg tttcccctga
    14101 atgcgggctt cggggtttcg gctcccccgg tcgccttatc atctttttct gccccagctt
    14161 ctatggttcg agtctccttt ttggcctctg ttttttccgt atcttcagaa gattccctgc
    14221 gtggagtcct ttcgattggc tcatctgtcg atcccccggc aggctccgct atcgtttgct
    14281 cctcgccttg ttggacagcc atctccttaa gcctttcctt tataagatcg atcagttttt
    14341 gttttttgtg cctggttacg ctcttgaccc ccaattcctt tccgaattcc cttaattgga
    14401 gaatcgtgtt tttttcaaga ttatttaaat ccaagatcta ttcctcactt tctttattat
    14461 acttattttt attattctgt tatcgatgta aaatgatttc attctgcagg acgccgtggt
    14521 cagtccatcc ttgaatatgg tctataattg ctgcacagac cccgtaggga catgcaattt
    14581 tacctactgc ctgttatacc cgacttgaat ctgcctgcta tggatgcggc attcgatccc
    14641 tttcgggata ttaaaatttc cgatattagg acaactttcc catgggtaac tgcttatttg
    14701 aaatacttct agactacagg tttttgaagt tatggctgaa gacacttaaa caaattaatg
    14761 gggcctgtat caaacgtaac tataaatgtt ctgtagtaca tgaaaagctc aaaccatatt
    14821 aatccatagt atagccatgg ttatatgttt taattcacaa atgggctata caccacttat
    14881 taaggcgagt ttttcttgat tttcggcttc gtatcctcaa tttcctaagc ttgagttttg
    14941 aataatcccc caatcgccct ccggacccca tctgcgggca aggcaatata gtttaaaggt
    15001 gggaaacctt gaatgggttt aataaataga ctccaattaa atggttgcca tcaataaaat
    15061 aatctatgag atgaaaccgt gtaatgatta ttactcaaaa agcattataa ctctacctta
    15121 accgctatat ccggtgttaa aacaatccta ctaatagtaa atgtcgttat tgatttaagt
    15181 agaacttgct ctataggcaa taatcatggg ataggaattg aacgtgattg ctcgaaggca
    15241 taccgatcaa taaaaactgc caaaggccct gtcaaagatt aagatatccg aaaagatcca
    15301 tataattgta ctctttagcc atcttcaaaa tgttttgttc atgttacgaa agagatccaa
    15361 aattgcataa tttgaaagac cgtttgatcc catattaagc cgatgtattc tccataacac
    15421 atagattcga gaaaatgttc tacaatattt gaaacataat gtaagatgat caatataaaa
    15481 caaaaggact aaaccatata ataacccatg aatataatat aaccattccg atggaaacgt
    15541 caacataaat atgaaaaaaa gagtgatttg tataaaaaat agttaatatt gtacaaaatt
    15601 ccatccctat tttgaatgtt gtcacctata agggaatttc aaagctatgt ccattggata
    15661 cagcatccat catagcccag gggccggcac catctttata cccaaagttt cggggccaaa
    15721 tgttagaggc tagagactgg gcagtaagcc aatctctaac acctgaattc taactcctaa
    15781 tgggcactaa tattaaccca aggatattaa ccgttaacaa atacctaccc aaacgtttaa
    15841 aacttaaaca aaccctgggg accgttttcc tgatagctca ccatcagttc attagaaccc
    15901 gcgtatttgc ctgaaatatc cagttcatac tttacatcca gcttcccctg gttttcatcc
    15961 atattggaat caattctggt ggggaaaatg cccatctcta ggttcccaaa aggcgtttcg
    16021 tagtaattga tatattttct gcccttttca aagacaaatc gagattcgtg ggctcccgta
    16081 cgctccaggg aaatccttcg atcttccacc attataaggg tcttagtccc ctccaggccc
    16141 gatattgcgc tctcttcgta ttcaatgtag tatgcgtcac cttttttata taaacgtcct
    16201 tccgttataa gctctatcct gttatcttct ccgtcgggat ttacttgatt gccccttatg
    16261 gataaaataa cctttttacc cccgcccatt ataactgacc cccctaaata aatctccctt
    16321 aataccccag cttttcgcac gatattcctt gggccttcct ccatggaaaa cgtgaaatca
    16381 aaacatttcg atttcgattt tcctagcgca ttttatctct ctgcccaaga aacgcccgcc
    16441 cagctcctca aatttctccc cgagatcgct gacatagaag tcgtactgtg cctcagtccc
    16501 gtcctcactc aggagtcctc tgtccttgag tatgcggcca acctccagcg ccgtaccaca
    16561 agcgggattt atgagtgtca catctggtcc catgaccttg cctatggtgt cctctaaaag
    16621 cggataatga gtgcatccta aaagaagggt atctatatcg ttgcacctca tactatctaa
    16681 atatctctcg gctgtcaaca acgcaatttt ggaattactc caaccttctt ccactatggg
    16741 tacaaataat gaacagggat tggtaaatac ccgaatccga ggatccaagc gggatatggc
    16801 cttttcgtat gccccgcttc gaatagtacc ttgggtgcct ataaccccca gacgcatatt
    16861 cctagtagaa gccacagctt ccctggctcc cggttcaatt acccccagaa tcggaatatc
    16921 gaacatttcg gtcaccatgt taaggctgat agagcttacg gtgttgcacg cgatgactat
    16981 catcttaatg tccttgctca aaagaaacct tatacactgc tgggaatatc taattaccgt
    17041 ttcattggaa cgggtaccgt atgggatcct ggccgtatcc ccaaagtata ctatatcctc
    17101 ttgggggagc catttcataa gttcctttac aacggtaagg cccccaagcc ccgaatcaaa
    17161 tacccctatg ggtctattgt ccatattcag tcctcctaga tgtttacgga aaaattctac
    17221 attggtacac aggtgccgta aaacattatc cttagttcgt atgttacaaa aaacacatta
    17281 ttttaacgta aaatcggata atccagccgt ttttacaatg gaaggtcttt tccgtatcat
    17341 atatagatgt gtttcatttt gaatctgtcc aaagccagct ggaccagcct gtctataagg
    17401 tccggatagg atatccccga agcctcccat aactcgggat acatgcttat ctgggtaaat
    17461 cccggcatgg tattgacctc gtttagatac accttaccgg tttgtctctt taggaaaaag
    17521 tccactcttg ctaggcccga acaacccagc gccttatacg ccttaatcgc caattccctg
    17581 atctcctggt tcttgtccgt gggcagaggg gcgggtatca atcgctggga ctctccccca
    17641 tcctcatact ttgcatagta gtcataaaat tcctccgagg gtataatctc gccaaggact
    17701 gaggccttag gatcataatt tccaaggacg gagcattcaa tctctcgtcc gtctataaat
    17761 tcctctacta ttatcttttc gtcgtattta gcggcaatgt ccaacgccgc cagtaattcg
    17821 tccctgtcac cggcctttga tatacccaca ctggagccca tattggccgg ttttacgaag
    17881 acgggataca aaaatgaatc ctcgatgata gctaccacct catcgggatt ttcccctatc
    17941 tcatgtctgt agactccgat ataatccccc tggggcaaat ccgcatgaga aaatatagcc
    18001 ttggcggtca atttgtccat gccggtggat gaggatagga catcacagcc cacatagggt
    18061 atatgggcca gttcgaagag tccctgtagg gtaccgtcct cgccataggg cccatgtata
    18121 acggggaaga ttacatccac atgaaaaatc ctgctgggat cttccataag aaaaaagccc
    18181 ctgtacgcag gatcccccgg aaataccaat gctttggaat cctcttccca ccgccctgtc
    18241 tttatccctt caaccgggcc gtcataatag cgccaatacc cttccttggt gatacctatc
    18301 agatatatat catatttatc cctgtcaata ttttctatga tagaggaagc agatgatagg
    18361 gatacctcgt gttccccgga tctgccgcca aataacactg ctatttttag tttttgcatc
    18421 gttataccct ccctatgcaa tagtaattta tgaattattc catttgaaag cctatcaccc
    18481 ccagtctgac gtgctgtttg ttttatatgg gatccggctt ctaacttcta atttccaact
    18541 tctaatctcc gactcctatc acattctaac ataacccatg gcttatatga agttccacat
    18601 acacaaaaaa aatgaacttt gtggtataga ccaaatttca caaaaagaga atcggagacc
    18661 taaattatct ccgattctct ttagattggt actctctgac tatttgcccg acagattctt
    18721 ttcagccctt tcgatagcct tccttacgat attaccacag tttctggaag agacattgcc
    18781 ccagccttcg cgctggacaa tatcgtggac tccaagttct ttggcaatct cggttttcaa
    18841 ctcctcagac atcaatcctc ttctgcttct agccatatca atacctcccc ggtcggctct
    18901 aattaattat agccgccaag agtatttttc cccgggagag gtacggatat gctgctaaaa
    18961 aactggttaa taatcatatg accatggtgc cgttatccgt attcagaacc ttcaatatgt
    19021 tttcctctct acccgtatcc gaattgatgt agatgataaa gttttcgtta tcaaattttc
    19081 ctttgaactc atagcattct atttcatttc cggatttgcc cgggatcacc gccaaccttt
    19141 ccgaggttat ctcaaggttc ctgtttatga gctttctcgc atcctccggg ctcaactttg
    19201 gttcctttag gtcccgttcc ctgtgggcca caagataatt gagtgcctca aagcctatgg
    19261 gatttcccgt atcaagggat atcttcacct ttatgaggtc tgggtagacg atgaagtcgc
    19321 cgtctttatg ggcaaaatta aacaccgcca ttcccccgtg atgctgctcg tatgtaggta
    19381 tcatgtcctt gtatcccttt tccctcagaa atttcttagc cgcatcctcc gcctttccaa
    19441 tgtccatgcc cgccccttgg acagcaccct cacagattag agataccacc ttaccgccca
    19501 ttttggagac ggaaacaaag tagggtccct ccccatcccg ggtcttaagg ttcacccccc
    19561 aagtctcaat ttcgccctgg gtctcggcac ctttatcggc actttccacc cgatttctgc
    19621 cgaggaagtc tatggctatc ctctccgctt ccttgtaatc tatttttttg cccgagaccc
    19681 gaagtttttt tggccttaaa agggtctcgg aaaaggggcc gtcgtatatg agggtgggat
    19741 attctatgga cgtctcctct atcttggtaa attgcttgcc taccaggtcc tgggatgcgt
    19801 ccatcaggct ttcattgctc atatccctca tctcctgcca ggtcaccatt cccttattga
    19861 cgttggactc taaatccttg agctcgttgt tcagccttac gctgctgttt tttagctcct
    19921 tgaggttctt catctcgtcc aggcttatgg gcttgccctc ccccgccttc atggtaagat
    19981 accggcaata atccgacagc tggcttagat attttgaagt cttgttaagg gctatatggc
    20041 ttagggggag ctgtcctaag tttgtgctgg cggaatccgc ctgcctccat acatcggaca
    20101 gcatcgctat gctctgtcct tggtccccgc tgaccatagc ctttgacagt ccggattcta
    20161 tgttgtccac ccttcccacc agctcataaa aggatttttt gtaaaggtta tcggtacgta
    20221 ttgtatggtc ctggttctcc aggtattggt tatagcccca aactccggtg attgccaggg
    20281 ccaccgccag caggccggta ataatccatc gactcatggt atcatccctt cagtgtttta
    20341 ctgttaaaac atcggaagca attgcattgg caattgcaac acattaaggc atcctatttg
    20401 caaggtttat gtagcgaaat ggtgcttacc tattaccagg tgtacaggcc tcgaccatat
    20461 ccacgcgctg gtggctttag ccgggttata gtaatatact gccccatagg tcggatccca
    20521 gccgtttaac gcgtcccggg cggcccttag ggattcgtcg tcgggagcta ggtttatctg
    20581 tccgtcggat acggcagtaa atgcgcccgg ctgatatata accccttcaa tggtgtttgg
    20641 aaatctcgga tccgccaccc tgtttagaac cacggcggcc acggctacct tgcccaaata
    20701 gggttcgcct ctggcctcac catggatagc ctgggccaga agatacacat tcccaccacc
    20761 acctcgatct ccgcccctag aaggcggggc agaagtaccg ccgccggtag ccggcggttt
    20821 ggtccccaag ttaatgccca tggccgctgc ggtctggttg cccaccgtac cgtctacagc
    20881 caagccattc cttgcctgaa atttcttcac ggcctcaaag gtcggcgccc cataatatcc
    20941 gtcgataggc ccgtcataat agccccactg ctttagcttt tgctgtacct caaacacctt
    21001 gtcaccatac attccccaat agaccagggt ctcctcggca tcattgccgg actgcacata
    21061 ttggacgatc ataactgcgg aaataaaaaa agttaacaaa atagttataa cgagaagcct
    21121 gttccttgtc ttcattccct acttcctttc gtcaattcag aattgaagaa ccccagaacc
    21181 ccaaaatcct ctaatctcgt tctatttttt gttaactcca aatactctat accagttaaa
    21241 atattcctaa tagcctataa tttccttcgc tttttcccac cattccgcga tcttaaatct
    21301 ataaaccaca tgaacgtcat ctcctaggga aggctgctcc gaaatgtctt cccccgtctc
    21361 ccaatccccg gtttcatccc cgccatcccc gtcatctttt gctgtcccgt gggttatatc
    21421 cacaaagcag aggggcggaa acataatgca ccaccagttg gcgcccttgc cctcccctat
    21481 tgttatccta agggcctcat agttccctgc cggtaatagc atatcgccat atgcctttgt
    21541 gggaaaatca aagtgcccta aactggccct taccttatag cccggcagat gcctgtccgt
    21601 ctcctgctgt gccgatgcct caatatctct aagcctttcc tctataatat ggcgactgac
    21661 acccagggag tcggcctgcc ctaataaagc tccgtattcc gccaataatc tgtccctgac
    21721 catcagctta actgcctggt cctgtggaga gtcactgtta gctataacat gaaatcgaat
    21781 gcatgcttgg ttatttccat gatttgaata tgtaccggaa gtgaacagaa agggcatgat
    21841 catcatcaac aggaaaaatg cccctcttct tttatacatc atcgataccc ccatacaaat
    21901 atactcttta tatggatagt attgacaata atatgtttat tatacgtcga tgttaatcca
    21961 gaagcctcat actattgatg gccctgttta tgagctcctc cttggtctca tggaatacat
    22021 cgctgcatga ggaccacccc acggaaattt caatatttcg catgcccgga tgaacccttc
    22081 catagtctgt gaccctttgc ttgaagttac gataaaattg tatggcctcc cctatctgtt
    22141 gtccgggtat gataaccgca aagacatagt cgctatacct gaccactatg tcctctacat
    22201 gggcacattc ccttaaaatt ttggcgagat cctggagcag cacgtttcct gcgatatgtc
    22261 cgtagacgtt gctgtagatc ttatgtccct gtatatctat catcaccaga gaaatcggat
    22321 agttgttttg cttaaactgt tgaaattgtt caaatgtatt gtcccaaaac ctgttaaagt
    22381 atctgacgtt atacaggttg gttaaagggt ccgtattggc ccaggtgtat agttggtcat
    22441 tggtctctaa gagccgatcc ctggattcat tgagttcggc tatcagggtt tcatttatgc
    22501 ccatggattc catcatcttt cggtgtttta actccatgtc ctctataagc aatcgtgcct
    22561 gccacagggt ccaaataaag aacccaaacc caaccgtcca atatatgggt ctgtgccata
    22621 tgccggtctt aaggcccgca tcgatggtat aaaaatcaac caaaataaac agcccggcgg
    22681 tcaggagact tatggggcat gaatacttat tttcatatac cagcgccatc cttacaagga
    22741 atgatataaa caatactacc accatgggtg aagaagatgc cgaatgaagg tataacagca
    22801 ccatcagctc cagagattct atcatcagat tataatagga gcccaatggc caaggatatg
    22861 catacttttt gaataggagt aacagggcgt aggacagact gacccagaaa aaaagttcgg
    22921 agaaaataaa ggcatttagg gggtttaaga taatgctcaa taatactgcc atgaatattg
    22981 agaataatct aattatccat tccagcttta gcatacctgt atttccctcc agtaaatcat
    23041 attattccag aaaatcatat tatgaaagta ttctacattt tttgatatta tccttcataa
    23101 ctatgcaaat attaaagcgt ttttgtcggt atggccgcct tttcccctag aattttcctt
    23161 ttgcctcctt caaatactgc tttgccaagc ctatttcatt gggatcgtct atatcgttgg
    23221 ctatttctgg gtaggggctt atgatagccc gggccttgat ttttaatagt tcagaaaagc
    23281 gctcttctat ccgggatatg gagagccttc ccactatcaa gagcagcagg aaacgaaatc
    23341 ccaacagcct acccagcttc cacggatttt ttctatgggc gataaatccc ttcgcaaatt
    23401 cagcgcagga tcctactact tttgggttta ggcctatgat gttcccgcct gtaaagatcc
    23461 cgtcccttaa cttgacatag gtcctcttaa ggccggggta gacggcgtca ttgacggtct
    23521 tgttgactat gggataacag agatcggcat tatataccct acatttctct atgaaatctg
    23581 aaactatatg cccgtctatc atgggaatat cggaagtggc taccagcaca aactcctcat
    23641 ctcgaaagga agagagcccc ttttttaggt tttcaaagag atgggtttcc tcctgaacaa
    23701 aatagtctac cctgcccgcc agcctggtct ttaaggtctc tgcagggcca ataatagcta
    23761 tcttacctac catttccgaa tccctcaggg catccacaac atattcgacc atccatttac
    23821 catatacatc cataagggat ttgttagtga ccccatcacc aaattctctt ttcccactat
    23881 ccccggccaa taccagtaca ttaatctttt ccacagcata gcccccttcc ccgaatgtgt
    23941 acattttgat ggaccgaacg aagtttcccc ttaggaaatt cgtcttcatc tataatgcat
    24001 tcaacgggtg aaatgcttta atatatccaa ttatacccta caaatcccat acaataaagg
    24061 gtccaaaacc agtttgttct tttccgggct gattgggctt gtcctgaggc gatgaatgca
    24121 cagatttccc ggcggttcag catccgtgga acatattgac attgccggcc tattgtatta
    24181 atatgcatgt aatgattgca gtgctgtagg gggagaattg ggtgataaag aaattcctag
    24241 aagataatat cctgattgct gacggcgcca tgggaaccta ttattcccac ctgaccggtg
    24301 cccgctctac tgtttcagag ctggccaata tagaagatcc gcgaaccgta gaggaaatac
    24361 ataggcagta catagaagcg ggatcgaaac taatacggac caacaccttt tctacaaatc
    24421 gctacactct gggcaaaaca cgtaaagagg tcagtgcgct tttgaccggc ggatatggta
    24481 tcgccaagtc ctctgtggga tcaagggatg tctttgtcgc cgccgatata ggtcctatcc
    24541 ctgagatgat tgaaggaaag gccgtcgaag gcgctgccat atatgatgaa tatttattta
    24601 tcgcagatac atttttaaaa ctgggtgccg atgtctttat atttgaaacc tttagcggca
    24661 cggaatacct agaggacctc tttgagttta ttaagtcgtc aaaccccgac tcctttatat
    24721 tggcccagtt tgcattaaat gcagaggggt ataccagaaa aggtataagc gcccggagga
    24781 ttcaaaaaca aataaaacag atcccttccg ttgatgccta cggctttaac tgcggggtcg
    24841 gtccggccca tctacataaa attatcaggg agctggattt ttccggcgat ttggtctctg
    24901 tccttccaaa ctcgggctat cccatcatcg tcaacgagcg tacggtctat accgaaaatc
    24961 ccgaatactt cgcggacagg atgatggata tcaaggacct ggggatcaat ataataggcg
    25021 gatgctgcgg aaccactccg ctccatattg atatgctgtc taaaagactt gattctgatt
    25081 ctgcacgaat tttcgcgact cctaagatag tcactgagaa accccatatt ccaaagaccc
    25141 ccacaaatcc atttgcccat aagctaaggg aagacaaatt tgtcattgcc gtggaattgg
    25201 atccgccctt cagcaccgat tttgataaga tggtggacgg ggcaagggaa ttgaaggaga
    25261 gcggtgtgga catattgacc atcgctgatt ctcccatggc ccggtccagg atagactcgg
    25321 gtatggtagc tgcgaagatt aaaagggagg ttggcataga caccatgccc catctatgct
    25381 gtagggatag gaatgttata tccataaaat catggttgct gggagcatat atagagggaa
    25441 tacgaaatct gctgataatt accggcgatc ccattccgag tccggaacgc aacgatatta
    25501 aaagtgtctt taacatgaat tccataaggt tgatagagat ggttgcagag atgaatcggg
    25561 aggtcttcgc ccaggaaccc ttttatttag ggggtgcgct gaacttaaac gtactcaaca
    25621 aggatgtaga ggtaggccga atgaagaaga agatgaagag cggggccaag ttctttttga
    25681 cccaacccat atttttagat tccactattg agtatctaaa agggataagg acaaatgcgc
    25741 agaccaagat tctagccggg ataatacctt tggtaagcta taggaatgcc gtctttttaa
    25801 ataatgagat acccggaatc catatccccg ataaattcgt caagatgttt tcaccggaaa
    25861 tgtctcggca ggaggcggag gatgtgggga tagagattgc agccgagatc atcggcaaag
    25921 taaaggagca cgtggacgga atctacctaa tcacaccatt taacaggacc ggtatgattg
    25981 caagaataat agaaaaaagt ctcagcccgg ctgaaaggag tccgttatga taataatagg
    26041 cgagaaatta aataccagta tagacgaggt gggtaaggca gtccaagata gcgatattga
    26101 tgcgatagag cagatagccc tatcccaagt aaaggcaggg gctgattata tcgatgtcaa
    26161 ttgcggtacc tttgtagaca gggaacccga gttgttaaag tggatggtaa ctgttctcgg
    26221 cgaatgcacc cataaacccc tgtgcataga cagccccaac ccccaagcca taggagccgc
    26281 cctaaaagtc tatgggggcc ataagcccat aatcaattcc atcacgggcc aaaaacgtag
    26341 gtatgacagc atactccccc ttgtgctgga atacgggaca tccgtcatag cgctgtgcat
    26401 ggacgacgac ggcatacccg gctccgcaga tgccagggta tccgtggcag aaaggcttgt
    26461 ggagaatcta accaatgacg gcgttgactt tgaggatatc ttcatagacc ctctggtcca
    26521 gcctatggct atggatcaga ccaacgggct tacaaccctt aggaccattg agggtataaa
    26581 gaaaagcctg ccgggtatca agaccctctg cgggctcagc aacatctcct tcggtctccc
    26641 ggatcgctcc tccatcaaca aggccttttt agtcctggct atccataagg ggttagatgc
    26701 cgctatctta gaccccctgg accgcggtat cgtttcattg tcctatgccg caaaactcat
    26761 ggggggtcag gacgaatact gccttaatta tttagaatcc attaaaagcc taaaggaaga
    26821 gtgaaaatcc tatggctttg aatccggtct tatgccggtg gaatcagctt aaggccttct
    26881 gcttataagg acaccatttt gaacggtctc tgcgacatat gtatgtcgat actccttttc
    26941 catcttcttt gcggccctac gggccgagga ttggtcctta aagacgccat agaccgtagg
    27001 cccgcttccg ctcatcaggc tgcccagagc cccttcatcc actaatcggg attttatctc
    27061 ggcaatttgc gggtgcattg gtatggtgac cccctctaag acatttacca ggttttcggc
    27121 tacgccggat acccgcccgg ctataaggtg ctctatcatc gccttattgt tcggtctttt
    27181 ctcgacataa tcgaggtcca atctaccgta tacctcccct gtggataccc gcaagtccgg
    27241 ccttactata accagccaaa tacttgtccc ggaggctatg ggcgttaata tgtccccaat
    27301 gccctcagcc agtgcagtac cccctgttat acaaaatggg acatcggctc ccagtctttt
    27361 ccctagggtc ttcaatgccc catcccccag attaaggttc cacagcctgt tgagccccag
    27421 caatactccc gcacagtctg cgctcccgcc tcccataccg gcggctatgg gaatgttttt
    27481 cctaataaag atcccagccc cctgagggat tccaaactcc gcctttaaga gctctgtcgc
    27541 ccgccaggca atgttccttt catccttggg cagaccctta acattgctgt ccagctcaat
    27601 tttcccgtcc gttaccctta taactatctc atcccatagg ttcaccgact gcattaccat
    27661 ctccacgtcg tgatagccgt cggcccgctt acccacgacg tccagggtca ggttgatctt
    27721 ggcatggacc ctaacgcaaa tactttccat ttcctcaaat cctttgcgaa ctaatgtatg
    27781 atattggtac aaaacatgca cagataagcg atacgttcag tagtcattat acccgaaaag
    27841 gccatggtta gcatccccca aacatagaga tggggaatac tattttgaca ataatattcc
    27901 ccatccgttt ttgatacgtg ggtaaactaa tatgataatt attattttca tagaaactag
    27961 actatagaag catccaattt tttaaatatc ataatcttgc tcccggcttc taggactccc
    28021 tcatcggcga tttcgttata ccttaatatg gtattgatag tcgtgttata tctctttgct
    28081 actgaccaca gattatcccc cggctgaaca aagtagatgt atatcccaga atccgcctcg
    28141 ccctctgcct ctatctcctc tatttccagc aggacttcct tttctatttc ctcggttgcc
    28201 tcacccgtta ccagcatggt tatcttaagt tctacctcat tgggtgagat tagggtatgg
    28261 cttagatatt gtaccacagc ctcacatagg cattccatat cctcattgat accctctatc
    28321 tccagggtct gggaaaaggg tatatccgcc tttatgctgc taatgcccaa cccgtcgtct
    28381 tcgggcttgt agagaagaat ccccgttaac accccgtcca ccactacttg gccctgacct
    28441 atccgatagt cggccactaa gggcctggct tccgcataga gaatcctccc tgcagggggc
    28501 aggttttcac cgagctcgat attctctctg ataatggact gggcctggcc cttgcccatg
    28561 gggcgggtta acattatctt ctgcttcacc agatttagtg cagtgccggg gctataggca
    28621 tcaattatca ggtcctcttc atggctttcg tagacccttc cttccataag gagtatcatg
    28681 tccacggaca ggatcctggt atccccaatg atatcctgtc ttaaggatac atcgagctcc
    28741 tgggcctcta ccttaacgtc gcagtccatt ccctgatatg cgccttggac ctctacaaaa
    28801 tggttaaaag ggatttggtt ttgcaaaagc tgtataggat cctgctcttc cccggattgg
    28861 tataatagtt ttaggtctat ctcgccttgg gctaggatcc tattatccgt aactctagtt
    28921 tccaagattt ttaccttggt gtcctttctc aaaaccctta tgacagaggg catgtcatcc
    28981 ggcaattcca catcctcacg aataatagtc tggccgctgc catcgccgcc ggtgaaaacc
    29041 aattccatcg gctctcttag ggcctgaatc ccatcttcat ccggaaaatc ttttagaact
    29101 tccaatgaca ataattcccg gaccctacaa tctatattga gtaccgcctt tacgccgagt
    29161 cttcggctgc tcaatatatc gtaatccaga tgctccagtt caaaggatat atcggctgtc
    29221 atcctgggct ttgcgccctc taaatccacg tactcgacga acccggtctc actttcggcc
    29281 tgggctatgg acttgctgtc tccaacgggt atgtacagta gattgtaacg caaaattccc
    29341 tctaccatca ccttgtcctg gacaacttcc ttacctgttc catataccac agcccagaca
    29401 tccagaatct tagctatctc gggttcttcc tcgtggacga taagctcccc ctccacaaga
    29461 tgctgtgata gctcttcccc taccagttgg tctattttta taagcccttt ttcgtaattg
    29521 atggccaatt ccattccccc ttttggaaat atttatagta agaatgacaa agatacagat
    29581 aaacattaag gatccgggct gcaagccgtg tgaatcaaaa tcctttcgcg tgtttctatg
    29641 gtatatgaac tataggtcgg atctgtttat ctttaattgt cccatatcta taaatataga
    29701 tggccacccc aaaatatgtt agattctttg attatttttg cataccacca attcaacgtt
    29761 ggaggttaaa acatcggaat aggtgtagca tagggtcctg gtgctttgtc cgtctatatc
    29821 gaccagcacc gtaaaaacgc tgggatatat atcatttata atcccttcgt ttacataggt
    29881 gcgtctgcgc cctctattgg cctttaattt aaccctttcg ccaatattgg actctaaagt
    29941 ctccctgact ttatccaaag catcaatatt tatcaccata tcacttccta tatatatttt
    30001 tatcatttgg atttgctgca aaactatttt gtttacagtt aaaacggaaa tgctttgcct
    30061 atagacaagc ctcttccata atatgcatag catatatatt ctacccatag tagggttttt
    30121 agattcattc gatgcaggta aatccggttt cactattgtt tatctgtaat aaccaatggg
    30181 ccgggattgt tgaagtaacc gtttaagatg ttttaacgga aaagatagta tgaaaaaaac
    30241 gctgctacct aatgg
//
LOCUS       DASRNX010000231        21315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_46842,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000231 DASRNX010000000
VERSION     DASRNX010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 21315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21315
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46842"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..986
                     /locus_tag="VFD89_09110"
     CDS             99..986
                     /locus_tag="VFD89_09110"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZJ58375.1"
                     /translation="MAVALGVFDGVHIGHQMLIRQLQLIRDSTGCKALVYTFLNNPME
                     FLSPRNAPPKIMTISERIKKLQRFGIDYLVLNPFDRGLASLAPQEFVEDALIKKYNIK
                     YLVVGHDFRFGHMARGDTALLHKLSQKHGFELVIIPPLSLGERVISSSFIRKLIQSEG
                     HMKKVSMVMGSHYTINGRVIYGYGRGKGLGFPTANLEFDVRKVIPKYGIYFTRVGVDG
                     TSYWGMTNVGINPTFNNKGLFIETHVLNYDGNLYGTKFKIEFLKRIRDEIRFTNVEDL
                     KEQITKDIKWAKNYVYKLQ"
     gene            1101..1364
                     /gene="rpsO"
                     /locus_tag="VFD89_09115"
     CDS             1101..1364
                     /gene="rpsO"
                     /locus_tag="VFD89_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZJ58376.1"
                     /translation="MDKAKKDQIINEYKLHDDDTGSPEVQIAILTDRINHLNEHLKTH
                     KKDHHSRRGLLMMVGRRRGLLNYLMKKDIGRYRDIIERLGLRK"
     gene            1808..3985
                     /gene="pnp"
                     /locus_tag="VFD89_09120"
     CDS             1808..3985
                     /gene="pnp"
                     /locus_tag="VFD89_09120"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZJ58377.1"
                     /translation="MDYKQYSIDLAGSAITVEIGKFGEQANGACTVRCGDTMVFVSAT
                     GSKEPREGIDFFPLSVDFEEKLYAVGRIPGGFIKREGRPTEKAILISRLIDRPIRPLF
                     PEGYRNDVQIVAMPLSVDPEYPPDIFAMIGSSIALSISDLPFMGPTGSVIVGLVDGEF
                     VINPVSTQREKSLMNLVVSGTKDAVMMVEAEAKEIPEDQMLEAIMFGHEYIKKLVEFQ
                     EKIVQEVGKEKLEFPAMEIDDELEAKVRSFASEKMGQAIRAFEKQEREGNLETVEREV
                     HEHFADEYPEQEKAIDDILENIKRGVVRGMILDEGIRPDGRTPAEIRPLFCEVGLLPR
                     THGSGFFKRGQTHVLTACTLGPMADVQILDGLWEDETKRYMHHYNFPPYSVGETRPMR
                     GPGRREIGHGALAERALEPIIPNEEEFPYAIRLVSEVMSSNGSSSMGSVCGSTLALMD
                     AGVPIKSPVSGIAMGLIKDEKTDRIAILSDIQGIEDFLGDMDFKIAGTSKGVTAIQMD
                     IKIKGIDREILVKALEAAKEGRMFIMQSMLNCIAEPRQELSPYAPKIITATIHPDKIR
                     DVIGPGGKVINGIIAETGVKIDIEDDGHVFIYSPNQEDAKRAFKMIEDITKDAEVGEI
                     YDGKVMRIMNFGAFVEILPGKEGLVHISKLAHERVGKVEDVVKVGDHIKVKVTDIDDQ
                     GRINLSRKDTLPPPEKNKDFPQNQRSDRPRDRSFRRPPRRDRD"
     gene            4235..5488
                     /locus_tag="VFD89_09125"
     CDS             4235..5488
                     /locus_tag="VFD89_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZJ58378.1"
                     /translation="MYKRKRLCNEIQVVTERIPHFRSVSIGLWFRAGSVYESEDQNGL
                     SHFIEHMLFKGTKRRTAKDIAQTLDAVGGQLNAFTAKECTCFYSKVLDEHLELSLELL
                     ADMVFNSTFCETEMEKEKGVILEEISMYEDSPNDVVHELLSANFFKSHPLGSPILGTR
                     ENVGGFTRQKLQDYFNRFYVPQNLVISIAGNYDETSLYQVLDKYFGNWTEPEHEFGKP
                     SLFTPGGGVFYRYKDIEQTHLCIGCPGIVLGDEDIYPLMVFNNIFGGGMSSRLFQRIR
                     EEHGLAYSVFSFPSSYTHGGLFTIYGGLRPSQVDQTMDLVVQEIRAIKKGNITREELS
                     MAREQLKGNYILGLESTNSRMTAIGRNQLLLGRTMTPDEVLTKIDNVTMDNIYKVLDG
                     MFLRESTAIAAVSKEDPTERLSDLF"
     gene            5715..6590
                     /gene="dpsA"
                     /locus_tag="VFD89_09130"
     CDS             5715..6590
                     /gene="dpsA"
                     /locus_tag="VFD89_09130"
                     /inference="COORDINATES: protein motif:HMM:NF006162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HZJ58379.1"
                     /translation="MESKLTFTVIGGDKRQIEVINLLLDGGHSVNALGLDGLIDGRIK
                     IYTGIEGELFNCHALLLPIPYNDALGNINAKTSTMKISPKTIMEGAQEHMPLLVLGKA
                     DKAFIDLASKKGMDYLDLTGEEAFAVLNAIPTAEGAIWRAMEMTNFTIHGSKVLVLGY
                     GRIGKSLSRMLKGIGARVTVVARKAEDLAWIEENGYTAVHLRDLIESLPSQNIIFNSI
                     PHLIMDRNKLLAVDRKAAIIDLSSYPGGVDFIAAEELGIKSSLDLGLPGKVAPKTSAE
                     IIIKVMMDSLRERKT"
     gene            6611..7189
                     /locus_tag="VFD89_09135"
     CDS             6611..7189
                     /locus_tag="VFD89_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HZJ58380.1"
                     /translation="MNLRGLRVGFCICGSFCTFDRLIPEIEKLVTQGADVTPILSDAA
                     YNWDTRFYSAKEFRSVIEGITGKKIISSVKEAEPIGPMRTMDVVVVAPCTGNTMSKLA
                     NGITDSAVTMACKAHLRNQGPIVIAISTNDGLGVNAKNLGELLIMKNVYFVPFGQDDP
                     VQKANSLVAKLDKLLPTIELAVEGKQIQPLLV"
     gene            7496..8725
                     /gene="dapG"
                     /locus_tag="VFD89_09140"
     CDS             7496..8725
                     /gene="dapG"
                     /locus_tag="VFD89_09140"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZJ58381.1"
                     /translation="MKIVVQKFGGTSLSSTEMLGRAVQKVINAKRGGFQPVVVVSAIG
                     RKGDPYATDTLINMNMEISKDIHPREMDLLMSCGEIISAVIMTNTLKSRGYEARAFTG
                     GQAGIITDQNYSQADILKIKPKKIIEAIESGRIPVVAGFQGITEQGDITTLGRGGSDI
                     TAVALGEAISAFSVEIYSDVDGIMTADPKIVPNASVLSQISYADILELASKGAKVIHP
                     RAIEYAIRGNIPVFIKSAYKCTPGTIVSFGQERTFGLDKTISRRVVTGIAHITGRAQI
                     TIHGNDPVINNHILAQLAVNHISIDIINIFPDKMIFTIEEEKKQNARGIIEENGCDYS
                     ILEDCSKISAVGSKMRGVPGVMSTIVAALTRNNIQILQSADSHTTISCLVRGEDTARA
                     VIALHEEFQLYKSEIGP"
     gene            8858..9580
                     /locus_tag="VFD89_09145"
     CDS             8858..9580
                     /locus_tag="VFD89_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HZJ58382.1"
                     /translation="MFLSKNEKNNDINDNSQDSGLPGDNIKEFGTTTLPKPPPNIHCL
                     TIIGQIEGHMVLPPQNKTTKYEHVIPQLVAIEESKEIEGTLLILNTVGGDVEAGLAIA
                     ELISTMSKPSVSLVLGGGHSIGVPLSVCTDHSFIASTATMTIHPIRMTGVIIGIPQTY
                     DYFNKMQERVVNFVIRHCNITRHKFKELMLRTGELANDVGTVLIGEEAVENGLIDQVG
                     GLRDAMGKLKELINRKKEDESL"
     gene            9577..9813
                     /locus_tag="VFD89_09150"
     CDS             9577..9813
                     /locus_tag="VFD89_09150"
                     /inference="COORDINATES: protein motif:HMM:NF025401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlzJ-like family protein"
                     /protein_id="HZJ58383.1"
                     /translation="MTFHSIIPIEDIFSINQLSPQDQTQIVQFSGIKAEVRFTGDNTY
                     EIQRIFTTNLKDYLRSELQPGTRIKAGYTIQNER"
     gene            10179..12482
                     /locus_tag="VFD89_09155"
     CDS             10179..12482
                     /locus_tag="VFD89_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZJ58384.1"
                     /translation="MAKRKAKTKKRKKNNRKYELMGVAIIGLGIFSLLSIYTSSAGIV
                     GMIFGRALKGLFGIIAYITPPLIILMGILTIMTYHKTVNKAKLGLTIFLIFLVFELIH
                     LLYINSFNRIDIWNFVVDSYNFGIDNQGSGALFSAPVYLISKWFGILGSYLCLIILLI
                     IDLLLLTNLSLKQLGVNVYGKISEKKTLFNRDNRDSQQMIREDRDFIGEVIELEPHSG
                     GQADPQHLKDSFKTRQNHLREQANGDDDLKIVEYDKSGEESLGVRGKESKSPAALKDN
                     KGTDDMKTQISQTQITDYSFPPITLLEEAKALKESKLSGAKVRNNAKLLEDTLMSFGI
                     SAKVLQVSKGPAITRYELQPAPGIKVSRIVNLSDDIALNLASPGVRIEAPIPGKAAIG
                     VEVPNRDISVVLLREVLESNAFLDHSSRISFGLGKDIAGKSIVADIARMPHLLIAGAT
                     GSGKSVCINSLIVSILYKASPKDVRLILIDPKVVELNHFNGIPHLLIPVVTDPKKAAG
                     ALNWAVQEMTSRYKLFASEGVKDLHNYNNKILAADGDGLPQIVVVVDELSDLMMVAPG
                     DVEDAICRLAQMARAAGIHLVIATQRPSVDVITGLIKANIPSRIAFAVSSNTDSRTIL
                     DMGGAEKLLGRGDMLFYPVGTSKPIRVQGCFVSEKEITGIVDYIKNIHDAPTYDLEVI
                     EEIAADREEQISEEYDELLPEAISIAIDAGQASISMLQRRLRIGYARAARMIDEMEVR
                     GIVSGFDGSKPRNVLISREEFEEDYAK"
     gene            12537..13085
                     /gene="pgsA"
                     /locus_tag="VFD89_09160"
     CDS             12537..13085
                     /gene="pgsA"
                     /locus_tag="VFD89_09160"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014281149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZJ58385.1"
                     /translation="MNLANRITFSRILIIPLFMVCLLGNFPYSQYIAALIFILAASTD
                     TMDGYIARKRNEVTNLGRFLDPLADKLLITSALVVLVEMGKVSSIVTIIIISREFIVT
                     GFRIIAASEGIVIAASWLGKIKTIVQIVSVVAILLDNFPFNLLGFPFDVISLYIAAII
                     TILSGIDYIYKNRELLKEKVMG"
     gene            13133..14374
                     /locus_tag="VFD89_09165"
     CDS             13133..14374
                     /locus_tag="VFD89_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HZJ58386.1"
                     /translation="MNAEIIGIGTELLLGQIVNTDAQYISQKLSQLGINVFYHTVVGD
                     NMERVKQALTIAAGRVDLIFTTGGLGPTMDDLTKEAIAEHLGLTLTVHQPTIDTLEEF
                     FTRRGRAISQNNYKQAHFPEGAIILPNNHGTAPGAIVEHNDKVFIILPGPPGELQPMF
                     EKHVIPYLEARSDEKIVSKVLKIYGIGESIMEEKIKDILTNQSNPTVAPLAGESELTL
                     RITAKTPRSNDPSSLIQPLETQIRERLGDVVYGVDNDTLESVVVNLLAGSNKTIATAE
                     SCTGGLIARRITSIPGASDIFMEGAVTYSNESKIKRLGVSRSTLIKYGAVSFETATEM
                     VAGILRTTGADVGIAVTGIAGPGGGTADKPVGLIYIAIGDKTGYLKAQEYNLLGERLR
                     VQQTAASIALDSVRRILLNIN"
     gene            14512..15537
                     /gene="recA"
                     /locus_tag="VFD89_09170"
     CDS             14512..15537
                     /gene="recA"
                     /locus_tag="VFD89_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZJ58387.1"
                     /translation="MQKQKALDMALVQIEKQFGKGSIMRLGEGTHLNVEVIPTGCLDL
                     DVALGVGGLPRGRVVEIFGPESSGKTTVALHVIAEAQKLGGTAAFIDAEHALDPSYSA
                     NLGIDLDSLLISQPDTGEQALEITEALVRSGAVDIVVIDSVAALVPKAEIEGEMGDSH
                     VGLQARLMSQALRKLSGIISKSNTVAVFINQLREKVGVMFGNPEVTPGGRALKFYSSI
                     RLDVRRIETLKQGTDMVGNRTRIRVVKNKVAPPFKQAEFDIIYGEGISKEGSILDLAA
                     SKDIINKSGAWYSYGETRLGQGRENARQFLKQNEDMRDEIELKVLKEFGLESPKINKN
                     SENQEKS"
     gene            15734..16132
                     /locus_tag="VFD89_09175"
     CDS             15734..16132
                     /locus_tag="VFD89_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZJ58388.1"
                     /translation="MEVNDLADESTQAGIDEYGKVTFADEVVATIAGLAALEVKGVSS
                     MSAGIVEKFGKKSPTRGVKVEVGEREAAVDLFMVVDYGVQIPEVAWNVQENVKKSIET
                     MTGLSVVEVNIYIQGIEFHKEISEQHSIKQ"
     gene            16216..16782
                     /gene="amaP"
                     /locus_tag="VFD89_09180"
     CDS             16216..16782
                     /gene="amaP"
                     /locus_tag="VFD89_09180"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HZJ58389.1"
                     /translation="MKISLFNRLLLTIYTLVIAIMSIAILAVFAAIGANILSSDSINS
                     FLAAIQSDWRFFLIGTLVSVFFLIVSVKLLFTGTGPKAPSSALLKHTDLGIIRISLDA
                     LEVMTHKAVNSFDEVRDSKIGILNEADGVKIRLKVSIMPDVILQDLSVSIQQKVKEYV
                     EGYSGILVKEVFVYIDNLAVPQQRAKVQ"
     gene            16795..17013
                     /locus_tag="VFD89_09185"
     CDS             16795..17013
                     /locus_tag="VFD89_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HZJ58390.1"
                     /translation="MYKELFNRIWQDHRGRILGVSLGLLAGIIIVTMGFWKTVILALC
                     AGIGYWIGGMTDRQERFIAFLDKILPKG"
     gene            17158..17586
                     /gene="nusB"
                     /locus_tag="VFD89_09190"
     CDS             17158..17586
                     /gene="nusB"
                     /locus_tag="VFD89_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZJ58391.1"
                     /translation="MSRIAAREVLMKLLYEKDIAGEHHNESFGNLSVEFQLDENDMKY
                     VKEILFSMDVKQGEIDGYIRKYAKDWTVDRMARVDLAIIRLALYELINKADIPASVSI
                     NEAVELAKKYGNEKSGSFINGILGKFIRMESVKPYTDTEE"
     gene            17700..18548
                     /gene="folD"
                     /locus_tag="VFD89_09195"
     CDS             17700..18548
                     /gene="folD"
                     /locus_tag="VFD89_09195"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HZJ58392.1"
                     /translation="MNANIIDGKAIAEKVRESLRYDIERLNREEGIVPGLTVILVGDD
                     PASQIYVRNKEKACMKVGIRSKVIYMDENIRQDDLLREIHKYNQDSNTHGILVQLPLP
                     DHIDEGLIIDAIDPKKDVDGFHPITVGKLFVGGEGFEPCTPKGIVTLIKETGIEISGK
                     HAVVIGRSNIVGKPVSIMLLRENATVTICHSKTKNLKEISKSADILIVAMGRPESIDN
                     EYIKEGAIVIDVGTTRVDGKLKGDVKFSEAVQKAGWITPVPGGVGPMTIAMLLKNTLQ
                     AALLNE"
     gene            18541..19773
                     /gene="xseA"
                     /locus_tag="VFD89_09200"
     CDS             18541..19773
                     /gene="xseA"
                     /locus_tag="VFD89_09200"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZJ58393.1"
                     /translation="MNRSVFSVGQINNYVKGLMDRDPLLQNLWIRGEISNFKLHSSGH
                     MYFTLKDDQSRIRCVFFKYHTTGLAFIPSDGMSVILGGNISIYTRDGQYQFYVEHMER
                     DGIGRLYAAFEVLKARLSEEGLFSDDFKKRLPPFPKKIGIVTSHTGAAVHDAIRVITE
                     RNKTVSILVIPVSVQGETAKDQISAAIDYANTRDDIDILIVGRGGGSIEELWAFNEEK
                     VARTIFRSRIPIISAVGHETDFTISDLVADVRAATPSAAGEIATSELTNICQALDRLD
                     EGLQYSMSKYMAEQDAKLVSLSDSYMLKYPERFFSSWEQRLDYNTEKMTGLAASYAQQ
                     KYDSFIGMISHLEALNPLTIMMRGFCILTDMNRKTTINSIDQLHEGMTAMIRLTDGIA
                     ACNITNKEKETGDENRSR"
     gene            19754..20023
                     /gene="xseB"
                     /locus_tag="VFD89_09205"
     CDS             19754..20023
                     /gene="xseB"
                     /locus_tag="VFD89_09205"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011746745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZJ58394.1"
                     /translation="MKTGVDNGISSLKFEDAMKELEIIVDRLEGGESPLTLEEFVELY
                     KRGVFLSQHCKKTLQQAQQEIKILTKNLDGNLEEVPFQQENHGGV"
     gene            20010..20903
                     /locus_tag="VFD89_09210"
     CDS             20010..20903
                     /locus_tag="VFD89_09210"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZJ58395.1"
                     /translation="MGVFNRQYDRLMGLITAYLNSSLEKMERYPETIFDSMEYSLFAG
                     GKRLRPIMLLAVHELLGGNIEESMPLAGGMEMIHTYSLIHDDLPAMDNDDFRRGRPTN
                     HKIFGEGMAILSGDALLNLAYETMLENALLYPDRLINHIRAINAIARASGIRGMVGGQ
                     VVDLESEGKPVTADTLKYIHDHKTGSLFIASISAGVMLENPSESAARALIEYGNSLGL
                     AFQVVDDILDVTGDRQEMGKNIGRDIDRGKATYIDKYGLERSRSIVKSLYERALDQLA
                     IFGPRGEFLSELAGFIINRHN"
     gene            20914..>21315
                     /locus_tag="VFD89_09215"
     CDS             20914..>21315
                     /locus_tag="VFD89_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HZJ58396.1"
                     /translation="MSYYTQLLYNNILWVSLLAWFIAQLLKVVFTLIFNKKLDIRRFI
                     GAGGMPSSHSALVVSLATSVGRFEGYDSPIFALALTFSLIVMYDAAGVRRAAGKQAAV
                     LNEILEQIQTSRSISEEKLKELLGHTPIEVIA"
BASE COUNT         6547 a         3789 c         5279 g         5700 t
ORIGIN      
        1 gatacagata acatgataag aatgagaaag gttttaggtt agaacagaat atgcgggtaa
       61 ttttcaatat tgatagacaa aatagtttgg gtgaacccat ggctgtcgct ttaggggttt
      121 ttgacggcgt ccatatcggc catcagatgc tcatcagaca attacaattg atacgggatt
      181 caacaggttg caaagccctc gtgtatacct ttttgaacaa tccaatggag tttttgtccc
      241 ctaggaatgc acccccaaaa atcatgacca tatctgaaag aatcaaaaaa cttcaacgtt
      301 ttggaataga ttatctggtg ctcaatccct tcgatagggg cttggcatcc ctggcacccc
      361 aagaatttgt cgaagatgct ctcataaaaa aatataatat aaagtatcta gtagtgggac
      421 atgatttcag atttgggcat atggctaggg gcgatacggc attactgcat aaattaagcc
      481 aaaaacatgg ttttgaattg gtgataattc ctccattatc cttgggggaa agggttatta
      541 gcagcagttt tatacgaaag ctgattcagt cggaaggaca tatgaagaag gtatccatgg
      601 tgatgggcag ccattatact ataaatggca gggtcatcta tggttatggc agaggtaagg
      661 gcctgggatt tcccacagcg aacctggaat tcgatgtacg caaggtgatt cccaaatatg
      721 gtatttactt cactagagtt ggtgtggacg gtacgagtta ttggggtatg accaatgtgg
      781 ggatcaaccc gacctttaat aacaaaggtc tttttataga gactcatgtt ctcaactatg
      841 atggtaatct atacggaaca aaatttaaga ttgagtttct gaagcggata cgggacgaga
      901 tcagatttac taatgtagaa gatttaaagg agcaaataac caaagatatc aaatgggcaa
      961 aaaactacgt ttacaaatta caatgaatgt gataatatag caatggaata gaggttaaat
     1021 caaccatatt cttggtctaa cgcccctcct gcgttttact aggattatgg gggtgcctaa
     1081 ttttgaggag gtgagagagc atggacaaag ctaagaagga tcaaattatt aatgaatata
     1141 agctacacga cgatgatacc gggtctcccg aggttcagat tgctattctg acggatagga
     1201 taaaccacct taatgaacat ctaaaaaccc ataagaagga ccaccattct agaagagggc
     1261 tattaatgat ggttggaaga agaagagggt tactcaacta tttgatgaaa aaggatattg
     1321 gacgttatag ggatattata gagcggttag ggcttaggaa ataatacttg gagcggaatg
     1381 tccgctccat tattctaatt agagggattt tacggaccct gattaatcag ggagatagcg
     1441 ataattgaat tttatatacg aatgattttc cgttacctta atctagtatc ggtactatac
     1501 ataaccttgg tctaatctga ggtggttatg tttttaatcc ggcgttgttt tagcctatag
     1561 tgggaatact aaaagtaacc gccttgttat tatgagcttt gacgcattat acatagagct
     1621 atgatcaacg gacaagcggt ttatgctaca ccttctagct gtggggaata aattactgct
     1681 atcccttaaa taagctaatt aacatcaatt gttaattgta attatatgat gagggaaggt
     1741 taatatagat aaacgattgt aagtaataca cggttgcgta catgtgatgc gaaaggagaa
     1801 ttaattgatg gattataaac aatactctat agatttagcc ggaagtgcta taactgtaga
     1861 gataggaaaa tttggtgaac aagccaatgg agcctgcact gtaagatgtg gtgacacaat
     1921 ggtatttgta tcagccacgg ggtcaaagga acctagggag ggaatagatt ttttcccatt
     1981 aagcgttgac ttcgaggaaa agctctacgc tgtaggcagg atcccaggcg ggtttatcaa
     2041 gcgcgaagga cgcccgacgg aaaaggccat tttaatttcc agattgatag atagacctat
     2101 cagaccccta ttcccagagg gttatcgaaa cgatgttcag atagtagcta tgccgctgtc
     2161 agtagatcca gaatatcctc cggatatttt tgcgatgatt gggtcctcca tagcattatc
     2221 gatatcggat ctacccttta tggggcccac aggttcagtt atcgttgggt tggttgatgg
     2281 tgaatttgtt atcaatcccg tctctacaca gcgggaaaaa agccttatga atttagtggt
     2341 ttccgggacc aaggatgccg ttatgatggt ggaggcagag gctaaggaaa tccccgaaga
     2401 tcaaatgctg gaagcaataa tgttcgggca tgagtatata aagaagctgg tggaatttca
     2461 ggaaaagata gtacaggaag tgggtaagga aaagctggaa ttcccggcaa tggaaattga
     2521 tgatgagctg gaggccaagg tcaggagttt cgcttcggaa aagatgggac aggcaatcag
     2581 agccttcgaa aaacaggagc gcgaagggaa tcttgagacc gtagaaaggg aagtccacga
     2641 acactttgcc gacgaatatc cggaacagga aaaggctata gatgatatcc ttgaaaatat
     2701 taaacgcggt gtagttaggg ggatgatact ggatgaaggt atcagacctg acggcaggac
     2761 accggcagag ataaggccac ttttctgtga agtaggccta ttacctagaa cccatggctc
     2821 cggattcttt aaaaggggac aaacccatgt ccttacggcc tgtactttag ggcctatggc
     2881 tgatgtacag atccttgatg gcctttggga agacgaaaca aagcgttata tgcatcacta
     2941 caattttccg ccctatagtg tcggtgaaac gaggcctatg agaggaccgg gacgaaggga
     3001 gatcggccat ggtgcgctgg cggaaagggc gttagaacca attattccaa atgaagagga
     3061 attcccctac gccattcgtt tggtctcgga ggtaatgagt tccaacggtt catcttccat
     3121 ggggagtgtc tgcgggagca ctttagcgtt aatggatgca ggggtcccaa taaaatctcc
     3181 cgtctcgggt atagctatgg gacttatcaa ggatgagaag acagatagga tagcaatatt
     3241 atcggatata caggggatcg aagatttcct aggcgatatg gatttcaaaa ttgcgggcac
     3301 ctcaaaaggg gtaactgcca tacagatgga tatcaagatt aaaggaatag atagggaaat
     3361 attagtcaag gccttggaag cagctaagga gggccgcatg ttcataatgc agagcatgtt
     3421 aaactgtata gcagaaccca gacaggaact atccccatat gctccgaaaa ttattactgc
     3481 cactattcac ccggacaaga ttagggacgt aataggaccg ggtggtaagg ttatcaacgg
     3541 tatcatagca gaaaccggtg tgaaaataga catcgaagac gatggccatg tctttatata
     3601 ttcgccgaac caggaagatg ccaaaagggc ttttaagatg atcgaagaca tcacaaaaga
     3661 tgcggaggtc ggtgagatct acgacgggaa ggtaatgcga ataatgaatt ttggtgcttt
     3721 tgtagagata ttacccggca aggaaggctt agtgcatatt tcaaagctgg cccatgagcg
     3781 agttggaaag gtagaggatg tagtcaaggt tggagaccat ataaaggtta aggttaccga
     3841 catagacgat cagggcagga tcaacctatc taggaaggat acgttgcccc cgccggaaaa
     3901 gaataaggat ttccctcaaa atcaaagatc tgacaggccc agggacagat cctttaggag
     3961 acccccccgg agggataggg attagaatta aaacataaac tttagcgagt ttatgttttt
     4021 ttatcatacg tccatacaag aggaatctaa aaactgacag gatgggccaa tggtgtttct
     4081 ttcaccattg gccttagcca tccggatcgt agggtggagt gcggggatcg ggtaaagggt
     4141 tcgttatgga gaacttgata gtagagccgg tttaaaaccc agtgtccgga gtaggcggca
     4201 ataaaatcca tattatacga agggtggcaa ttcgatgtac aaaagaaaaa gattatgcaa
     4261 tgaaatacag gtagtaacgg agagaatccc ccattttagg tccgtatcta tcggcctatg
     4321 gtttagggca ggttctgttt atgagagtga ggaccaaaac ggtctatcac actttataga
     4381 acatatgctg tttaagggca caaaaaggag aacggcaaag gatatagccc aaacccttga
     4441 tgctgtcggg ggacaattaa atgcgtttac tgccaaggag tgcacatgtt tctactctaa
     4501 ggtattggat gagcatttgg aattatcatt ggaactcctt gcggatatgg tcttcaactc
     4561 caccttttgt gaaacggaga tggaaaagga aaagggtgtt attttggagg agatctctat
     4621 gtacgaggat tcccctaatg atgttgtcca cgaactgctc tcagctaatt tcttcaagtc
     4681 acatccattg gggtctccaa tattggggac gcgggagaat gttgggggct ttaccaggca
     4741 aaagttgcag gattatttta accgattcta tgtgccacaa aacctcgtta tatctatcgc
     4801 cggcaattac gatgagacat cattatacca agttttggat aaatactttg gaaattggac
     4861 agaacctgaa catgaatttg gtaaaccctc attatttacc ccgggagggg gagtatttta
     4921 caggtacaag gatattgaac agactcatct gtgtattggt tgtcccggaa ttgttctggg
     4981 tgatgaggat atttatcctc taatggtttt taataatata tttggcggcg gaatgagttc
     5041 caggctgttt caaagaatta gggaagagca tggactcgcc tattccgtat tttcgtttcc
     5101 atccagctat acccatggag gattatttac aatctatggg ggtttgagac ccagccaggt
     5161 tgatcagacc atggatcttg ttgtacagga aataagggcc atcaaaaagg gtaacataac
     5221 tcgtgaagaa ctttctatgg cacgggaaca gttgaaaggc aattatatac ttggtctgga
     5281 gagcaccaac agtcgcatga cggcgattgg aagaaatcaa ctcctattgg gtagaacgat
     5341 gaccccggat gaggtactta cgaagattga caatgttacc atggataaca tatataaggt
     5401 cttggatgga atgtttctga gggagagcac ggctattgcc gcagtatcaa aagaggatcc
     5461 gactgaaagg cttagtgact tgttctaagt cgagctataa tatgcatatt caaaatccat
     5521 catttgacgg gggcttattg cctcctattt ttttgcactt aacccacttt tacctcatac
     5581 tatgatatta aatatgtttt cattcagaaa ttcttttacc atgtttaagg caaagttaac
     5641 tatggtttac catagaaaaa aatcaaagct atttatggga tagcgttggc aaaatgcttg
     5701 cgagggggat ataaatggag tctaagttaa cctttaccgt catcggcggc gataaacgcc
     5761 agatcgaagt cataaatctg cttctggatg ggggacatag tgtaaacgcc ctcggcctcg
     5821 atggtttgat tgatggtaga ataaaaattt acactgggat agaaggggaa ttattcaact
     5881 gtcacgcgtt gcttttaccg ataccctata acgatgcatt gggtaatata aacgcaaaga
     5941 cgtcgaccat gaagatatca cccaagacaa taatggaagg tgcccaagaa catatgccct
     6001 tattagtttt gggtaaggca gacaaagcat tcattgacct ggcttctaaa aaaggaatgg
     6061 attatctgga tctgaccggt gaggaagctt ttgctgtgct aaatgcgata cctacagctg
     6121 aaggggccat atggcgggca atggagatga ccaattttac tatccatgga tcaaaggttt
     6181 tggttttagg ctatgggagg ataggaaagt cattgtccag aatgttaaag ggaataggtg
     6241 caagggtaac cgtagtggcc cgcaaggcgg aagatctggc atggattgaa gaaaacggat
     6301 atacggcagt ccatttaaga gatctgatag aatcccttcc ttcacagaat attatattta
     6361 attctatccc ccatttaatc atggatagaa ataaattgtt agcggtagat agaaaggcag
     6421 ccattattga cctatcctct tacccgggcg gtgtcgattt tattgcagct gaggagctgg
     6481 ggataaaatc tagtttggat ttaggcctgc cgggcaaggt ggcacccaag acttctgcag
     6541 aaataattat taaggtaatg atggatagcc tccgagagag gaagacatga atttgtatgg
     6601 gggtgtagat gtgaatttaa ggggattaag ggttggtttt tgcatatgtg gatccttttg
     6661 tacatttgat cggcttatac ccgaaataga gaaattggta acgcaggggg cagatgttac
     6721 gcccatttta tccgatgcgg cctataattg ggatactaga ttctattctg caaaagagtt
     6781 taggtccgtt atagaaggaa taacgggaaa gaagataata agcagcgtta aagaggcaga
     6841 acctatagga cctatgagga ccatggatgt agtggtggta gcaccttgca caggcaatac
     6901 catgagcaag ctagctaatg ggattaccga cagcgcagtc accatggcat gtaaggccca
     6961 tttaagaaac caaggcccaa tagtcattgc gatatccacc aatgacggtc tgggcgtaaa
     7021 cgctaaaaat ctgggggagc ttctaattat gaagaacgtt tactttgtcc catttgggca
     7081 ggatgacccc gttcagaagg ccaattccct cgtcgccaag ctggataaac tccttccgac
     7141 tatagagttg gctgtagaag gcaagcaaat acaaccattg cttgtttgac gccagggtgg
     7201 aatggtatat gtaattttcg attaacttta ttgatttttg ttttatttgg ctatattatt
     7261 aatgtaagag catatttatc ccagaaaact tcgtagcctg tcatggcgct ggtgttaaaa
     7321 tcgagtttgg ccggctatgt tttctgggat tggattatgg taaaatccta attagtccaa
     7381 tttcagcaaa caaggttata tataggttcc aacgattagg tttaagatgt gattgaattc
     7441 cattgaggta ataaccggct gtgattcctt gtttattata gatcggagga ataacatgaa
     7501 aatagttgtt caaaaattcg gcggtacttc attatccagc actgagatgc ttggcagagc
     7561 tgtgcaaaaa gtgattaatg ccaaaagggg cggattccaa ccggtggttg tggtttctgc
     7621 cataggcaga aaaggggatc catacgccac tgatacattg attaatatga atatggaaat
     7681 tagtaaagac atacatccta gggaaatgga tttacttatg tcttgcggag aaataatctc
     7741 ggctgtaatt atgacgaaca ctttgaaaag caggggttac gaggctagag catttaccgg
     7801 ggggcaggcc ggaattataa cggatcagaa ctattcccaa gctgatatat tgaagattaa
     7861 gcccaaaaaa atcattgaag ccatcgagag cggaagaatt cctgtggtag ccggttttca
     7921 aggaattaca gaacaaggtg atataactac cctaggcagg ggtggaagcg acataacagc
     7981 ggttgcttta ggcgaagcga tttcggcttt ttctgttgaa atatattcgg atgtagatgg
     8041 aataatgacg gcggatccca agattgtacc caatgccagc gtgttatccc aaatcagtta
     8101 tgcggatatc ctggagctgg ccagtaaagg tgcaaaggtc attcacccga gggccattga
     8161 gtatgccata aggggaaata ttcctgtatt tattaaaagc gcttataagt gcacccccgg
     8221 tacaatagta tcctttgggc aagagaggac ctttggttta gacaaaacga tttcaagacg
     8281 ggttgtgaca ggaatcgcac atataacggg cagggcacag attaccatcc atgggaatga
     8341 tcccgtaatt aataaccata ttttggccca attagctgtt aatcatatta gtatcgatat
     8401 cataaatatt tttcctgaca aaatgatttt tactatagag gaagaaaaga aacagaatgc
     8461 cagaggcatc atagaagaaa atggttgcga ttactctatc ttggaggact gcagcaagat
     8521 atccgctgta ggcagtaaga tgaggggagt gccgggggta atgtctacta tcgtagcggc
     8581 attgacaaga aataatattc agattctgca gtctgcagac tcccatacca ccatatcatg
     8641 cctggttagg ggggaagata ctgccagggc agtaatagcg ctacatgaag aatttcaact
     8701 ctacaaatca gagattggtc cttaaaggac ttttttttta taatagggat acaataagaa
     8761 aaatattaac tttgacttga gattatatat atcccatgta aggttccgag agtattctta
     8821 gcactaaagt aaattattgc agatagggga aaatgccatg tttttatcta aaaacgaaaa
     8881 gaataatgat attaatgata attctcaaga ttccggttta ccaggtgata atatcaagga
     8941 atttggaact acgaccttgc ctaagccccc gccaaacata cactgtctta ctattattgg
     9001 tcagatagaa gggcatatgg ttcttccccc acaaaacaaa acaacaaaat atgagcatgt
     9061 aattccccaa ctggttgcca tagaagagag caaagaaata gagggcaccc tattaatatt
     9121 gaacacggta ggtggggacg ttgaagcggg gcttgcaata gcagagctta taagcactat
     9181 gtccaaacca tcggtttcat tggtgctggg gggtgggcat agtattggag tccccctatc
     9241 ggtgtgcaca gaccattctt tcatagcatc caccgctacc atgacaatac acccaattcg
     9301 aatgaccggt gtaataatcg gtatcccgca aacctacgac tattttaata aaatgcagga
     9361 gagggtcgtg aattttgtta tcaggcactg caatataacg aggcacaagt tcaaggaact
     9421 catgcttagg accggggaac ttgctaatga tgtgggtact gtattaatag gagaggaagc
     9481 agttgaaaac ggcttgattg atcaggtagg tggcttgagg gatgcaatgg gtaaactaaa
     9541 ggagctaata aatcgcaaaa aggaggacga gagcctatga ccttccattc tattataccc
     9601 atcgaggata tattctccat aaatcaatta tcaccccaag atcaaacgca aatagttcaa
     9661 tttagcggaa tcaaggctga agtgagattt acgggggaca atacctacga gatacaacga
     9721 atttttacaa cgaatttaaa ggattattta aggtcggaat tacagccggg aactagaata
     9781 aaggctggtt atacgataca aaacgaaaga tgaccatagt ggataccttt tttatacaaa
     9841 accggtggta aggtatgggt aaaggtggta taatagtttc atgccatatg attcccctat
     9901 gaagttctgt atacaatgga tagtattaga acaatccata caagagccat atattgtata
     9961 tagatgggtt atatcagtct tggtggagac attcatgtaa gtagttctcc acctgggaag
    10021 ctggggactg ttttggtttt taattgacgg gatattcaga tattggggtg ttggtatttt
    10081 ttactcctgt attttctgat atttggatga ggtacgattg aagatgatta tttcttgtct
    10141 aagagacgag gatggataaa aaatactgag gtggttctat ggccaaacgg aaggctaaaa
    10201 caaaaaaacg taaaaagaac aatcggaaat atgagctaat gggtgtagca ataataggtt
    10261 tggggatttt ttccctgctt agcatataca cgtcttctgc agggatagta gggatgattt
    10321 ttgggagggc ccttaaggga ttgtttggta ttattgctta tattacgccc cctcttataa
    10381 ttctaatggg gatactcact ataatgactt accacaagac cgtgaataaa gccaagctgg
    10441 ggctaactat ctttttaata ttcttggttt ttgagttaat acatctcctc tacataaata
    10501 gctttaacag gatcgatata tggaattttg ttgttgattc atataatttt ggtattgata
    10561 accaaggctc gggggcttta ttttcggccc cggtctattt aatctcgaaa tggtttggaa
    10621 tattgggaag ttacctttgt ctcataattt tattgattat cgatctttta ttgcttacga
    10681 acctttcatt aaaacaattg ggtgtcaacg tgtatgggaa gataagcgaa aaaaaaacct
    10741 tatttaaccg tgacaaccgc gacagccaac aaatgattag ggaagatagg gattttatag
    10801 gtgaggtcat tgagttggaa ccccatagcg ggggtcaagc cgacccccaa catttgaagg
    10861 attcatttaa gacgagacaa aaccatttgc gggaacaggc caatggggat gacgacctaa
    10921 aaatagttga atatgataaa tccggcgagg aaagcctggg tgtcaggggg aaagagtcaa
    10981 agtcccctgc agcccttaag gataataagg gcacggacga tatgaaaact caaatatccc
    11041 aaactcaaat tacggattac tcatttcctc ctattacctt attggaggag gccaaagccc
    11101 ttaaggaatc caagctcagc ggggcaaagg ttagaaataa cgccaagttg ttggaggata
    11161 cgttgatgag cttcgggata tccgcaaagg tactccaagt cagcaaggga ccagctatta
    11221 ccagatacga attacaacct gcgccgggta ttaaggttag taggatagtt aacttatccg
    11281 atgatattgc attgaacctt gcaagcccgg gtgtaaggat tgaagcgccc atcccgggaa
    11341 aggcggccat aggtgtggaa gtacccaaca gggacatttc agtggtccta ttgcgggaag
    11401 ttttagaatc caatgcattc ctggaccact cttcgagaat atcattcggt ctgggaaagg
    11461 atatcgcggg aaagtcaatt gtagctgata tagcccggat gccgcacctg ctaatagcag
    11521 gcgctaccgg ttctggaaag agcgtgtgta taaactctct aattgtgagt attctttata
    11581 aagcctcacc caaggatgta aggctaatac ttatagatcc caaggtggta gagcttaacc
    11641 attttaatgg aatacctcat ttattaattc ccgttgtgac ggatcccaaa aaagctgcgg
    11701 gggctctaaa ctgggctgtt caggaaatga ccagccgata taaattgttt gcaagtgaag
    11761 gggtcaagga tcttcataat tataacaata aaatccttgc tgccgatggg gacgggttac
    11821 cccagatagt ggtagtggtt gatgagttat ccgacctgat gatggtagcc cccggggacg
    11881 ttgaagatgc tatatgcagg cttgcccaaa tggcaagggc cgccggcatt catttggtta
    11941 ttgctaccca acgaccttca gtcgatgtaa taacggggct aataaaggcc aatattccat
    12001 caagaatcgc ctttgctgtt tcctccaata cggattcaag gactatattg gatatgggag
    12061 gggccgaaaa attgctgggc aggggggata tgttattcta tcccgtaggg accagtaagc
    12121 ccattagggt tcaagggtgt tttgtatccg aaaaggaaat tacgggcatt gttgattaca
    12181 taaaaaatat acatgatgca ccaacctacg acttagaggt tatcgaagaa atcgccgccg
    12241 acagggagga gcagatttct gaagaatatg atgagttatt acctgaggcc ataagcatag
    12301 cgatagatgc gggtcaggcc tctatttcaa tgctgcaaag gcggcttagg atcgggtatg
    12361 caagggctgc ccgcatgatt gacgaaatgg aggtaagggg gattgtcagc ggctttgatg
    12421 ggagtaaacc aagaaatgta cttatatcaa gagaggaatt tgaagaagat tatgcaaaat
    12481 agtctatatc cgccgaagca acccgaatca gcgattataa tttttggggg atcgtcatga
    12541 atttagcaaa tagaataaca ttcagcagga ttttaattat accattgttt atggtgtgtc
    12601 ttctgggaaa ctttccatat agtcagtata ttgcggcttt aatatttatc ttggcggcaa
    12661 gtaccgatac catggatggg tatatagcgc gcaagaggaa tgaagttacc aatctcggca
    12721 ggtttttaga tccactggcc gataaattgc tcattacatc cgctctcgtt gttttagtag
    12781 agatgggtaa ggtttcatcc atagttacaa taatcattat cagcagggaa tttattgtaa
    12841 ccgggtttcg tattattgcc gcttccgaag ggattgttat tgcagccagt tggcttggca
    12901 agattaaaac cattgtacaa atcgtatctg tagttgcgat actccttgat aatttcccct
    12961 tcaatctttt ggggttcccc tttgatgtaa tatccctata catcgccgct ataataacca
    13021 tactatctgg catagactac atatacaaaa acagggaact actgaaagaa aaggttatgg
    13081 gctagattct gttatgggac tctattaaaa ctttcttata gggggattat gaatgaacgc
    13141 tgagattatt ggtataggca ccgaattgtt attaggacaa atcgtaaata ctgatgcaca
    13201 atatatatct caaaagcttt cccaattggg catcaacgta ttctatcata ccgttgtcgg
    13261 tgacaacatg gagagggtta agcaggcgtt gaccattgcc gccggtaggg ttgatctaat
    13321 atttactacc ggaggtctgg gccctacaat ggacgatttg accaaggaag cgatagcaga
    13381 gcatttagga ctgacactga cggtacacca accgactatt gacaccttgg aagaattctt
    13441 tacaagaagg ggcagagcca tcagtcagaa caattataag caggcccatt tcccggaagg
    13501 ggctataatt ttgccaaata atcatgggac tgcgcccggc gctatagtgg agcataatga
    13561 taaggtcttt atcatcctcc cgggcccccc cggcgaatta cagccaatgt ttgaaaagca
    13621 tgttattcca tatctggaag ccagaagcga tgagaagatt gtttccaagg tcctgaagat
    13681 atatgggatt ggggaatcaa tcatggagga aaagattaag gatatactga ccaatcagag
    13741 caatcctacc gttgcgcctt tggcgggcga atccgaatta actttaagga taacggcaaa
    13801 aacgcctcga agcaatgatc catcttccct gattcagcca ctggaaaccc aaataaggga
    13861 gaggttaggg gatgtggtct atggtgtcga caatgacacc ttagaaagcg tggtggttaa
    13921 cctgttggcc ggatcaaata aaactatagc cacagctgaa tcatgtacag gcggtctgat
    13981 agcgcgaagg ataaccagca taccgggtgc atccgatatt ttcatggaag gcgctgtaac
    14041 ctacagcaat gaatccaaga taaaacgact gggagtatca aggagcaccc taattaaata
    14101 tggtgctgtc agttttgaaa cagcgacgga aatggtagca gggatattga ggacaactgg
    14161 tgccgatgtg gggatagcgg ttacgggaat tgcaggtccc ggcggtggaa cggcggacaa
    14221 accggtaggt cttatatata ttgcaattgg cgataaaaca gggtatttga aggctcagga
    14281 atataatcta ctgggggaaa ggttaagggt tcaacaaacc gcggcatcca tcgcactgga
    14341 ttcggtaagg cggatattgt taaatattaa ttgagcacaa aattgttaaa ttccattgac
    14401 ggggccttcc ggttatcata taatataagg gaacaaatgt tcggatcaga cggtgatcta
    14461 tcctaatatc aggaaaggca ggtgaacccc gaaaaacttc ggggggtgtg tatgcagaaa
    14521 caaaaagcgc tggatatggc cttggtgcaa atcgaaaagc agtttggtaa ggggtcaata
    14581 atgaggttgg gtgagggtac ccatctgaac gtagaggtaa taccaacagg gtgcttggat
    14641 ttagatgtcg ctttgggcgt tggcgggctg cctaggggca gggtggtaga gatatttggg
    14701 ccggaatctt cggggaagac taccgtggct ttgcatgtaa tagcagaggc ccaaaaactg
    14761 gggggtactg ccgcatttat cgatgctgaa catgccttgg atcccagcta ttccgcaaat
    14821 cttgggatag atctggacag cctcttgatt tcacagcccg acaccgggga gcaggcttta
    14881 gagataactg aggcgctggt tcgtagcggt gccgttgata tcgttgttat tgattccgtt
    14941 gctgctctgg tacccaaggc ggagatcgaa ggcgagatgg gtgattccca tgtgggttta
    15001 caagctcggc ttatgtccca agccctccgg aagctctcgg gaatcatcag taaatccaac
    15061 accgtcgcag tatttatcaa tcagcttcgt gaaaaggttg gagtaatgtt tgggaaccct
    15121 gaggttacac ctggtgggcg tgcccttaaa ttctattctt ccattcggct ggatgtacgt
    15181 aggattgaga ccctaaagca aggcacagac atggtgggta acaggacaag gattcgggta
    15241 gtaaagaata aagttgcacc ccctttcaaa caagccgaat ttgacattat ctatggggaa
    15301 ggcatatcca aagaggggtc tatattggat ttagccgcct ctaaggatat tattaataag
    15361 agtggggcct ggtattccta cggcgagaca cgattgggtc aaggacgaga aaacgccaga
    15421 caattcctaa aacaaaatga agacatgagg gacgaaatag aattaaaggt tttaaaggaa
    15481 tttggattag aaagtccgaa gataaataag aattcagaaa accaggagaa atcttgatga
    15541 gagaatcccg atagggattt tttcatctat ggggcaaatt gttttataca tatttaatat
    15601 tgattgtttg taaagaggta tttgttttgt tataatatac tatatatgtt acggaaaaga
    15661 attttagtta taagaccgtt taggtagaca tcgtggcttt atggatacat tggtgaagta
    15721 ggagataaaa agtatggagg tgaatgattt ggcagatgaa tctactcagg ctggtattga
    15781 tgaatatgga aaggttacct ttgccgatga ggtggtggcg acgattgccg gtttagcggc
    15841 acttgaagtt aagggtgtat cttctatgag cgccggaatc gtcgaaaagt ttggcaaaaa
    15901 aagcccgacc agaggggtaa aggttgaagt tggtgaaagg gaagccgcag ttgatctgtt
    15961 catggttgta gactatggag tacaaatacc cgaagtcgca tggaatgttc aggaaaatgt
    16021 caaaaagtcc atcgaaacaa tgacggggct atcagtggtg gaagtgaata tatacataca
    16081 gggtatcgaa tttcacaaag agatcagcga acaacatagt ataaaacaat aatccgttaa
    16141 cctcctggct ctttcagggg gttaatgcat gtgtatttat gacaatatac gtagataaag
    16201 ggaggcctga gcaggatgaa aataagtttg ttcaatcgct tattattaac gatctataca
    16261 ttggttattg ctataatgtc cattgctatt ttagcggttt ttgcagctat aggtgctaat
    16321 atactttctt ccgacagtat aaatagtttt ctcgcagcaa ttcaatccga ttggcgtttt
    16381 tttctcatcg gcaccctagt gtccgtattc ttccttattg tgagcgttaa attgcttttt
    16441 accggtaccg ggcctaaggc tccaagcagt gccctgctga agcatacaga tctgggcata
    16501 attcgcatat ccctagatgc attggaagta atgacgcata aggcggtaaa tagctttgac
    16561 gaagtaaggg attcgaagat aggcatactg aatgaggctg acggagtgaa gatacgatta
    16621 aaggtatcga ttatgcctga tgtaatcttg caggatttgt ctgtctctat acaacaaaag
    16681 gttaaggagt atgttgaagg ttattccgga atcttagtta aagaggtctt tgtttatatc
    16741 gacaatcttg ctgtacctca gcagcgagca aaggttcaat gaggggggag ccggatgtat
    16801 aaagagcttt ttaataggat atggcaggat cataggggaa gaatcctggg ggtgtccttg
    16861 ggattattag ctggaattat aatcgtgacg atgggttttt ggaagacggt tatcttagca
    16921 ttatgcgccg gtattggtta ttggattggt ggaatgaccg atagacagga aagatttata
    16981 gcttttttgg ataaaatatt gcccaagggt tgaataattt tttaattggt agtggtaata
    17041 tactggattt gagatgtttt tagcaaccaa tatgctatga aaagcacctt atttttgtat
    17101 gaccttttgc aatatatcca aatagtaaac ctacagggat gactcggagg agaattaatg
    17161 tctagaatag cggcaagaga agtactcatg aagttgctat atgaaaaaga tatagccgga
    17221 gaacaccata atgaatcctt tggaaatctt agtgttgaat ttcaactgga tgagaatgat
    17281 atgaaatatg taaaggaaat cctattttcc atggatgtaa agcaagggga aatagatggc
    17341 tacatacgca aatatgcgaa ggattggaca gtagacagaa tggctagagt tgacctggct
    17401 ataatcaggc tggctttata tgaattaata aacaaggcgg atatacccgc cagcgtatct
    17461 ataaatgaag ctgttgaatt ggcaaaaaaa tatgggaacg aaaaatccgg gtcttttata
    17521 aatggaattt taggcaaatt catcaggatg gaatcggtta agccctatac agatacagaa
    17581 gaataggata aaaatctaga tccttagcga tcccgtattg atctttttta aataaggatt
    17641 aaaagacttg ttataaatat caacatggga ttcgtttcca cgggtaaagg gaggcaaata
    17701 tgaacgcaaa cataatagat gggaaagcca tcgccgaaaa agttagggag tcattaaggt
    17761 acgacataga aagattaaac agggaagaag gcatcgtgcc gggactaacg gttatcctgg
    17821 taggtgatga ccccgcgtct caaatatatg tgagaaataa ggaaaaggcc tgcatgaagg
    17881 taggaattcg ttcaaaggtt atctatatgg atgaaaatat ccgacaagat gatctattga
    17941 gggaaattca taaatacaac caagatagta atacccacgg gatcttagtc caattaccct
    18001 tacctgatca tattgatgag ggattaataa tagatgctat tgaccccaag aaggatgtcg
    18061 atggctttca tcccatcact gttgggaaac tttttgtcgg cggggaaggc ttcgaaccat
    18121 gtacaccaaa gggtattgtc accttgatta aagagaccgg tatagaaatt tccgggaaac
    18181 atgcagtggt gatagggcga agcaatatcg tcggcaaacc cgtgtctatc atgctcctaa
    18241 gggaaaatgc cactgttacc atatgtcact ctaagacaaa gaaccttaag gaaatatcaa
    18301 aaagtgccga tatactgata gttgctatgg gccgccccga atccatagat aatgaatata
    18361 taaaagaagg ggctatagtc atcgatgtag gcacaacaag ggttgatggt aaactaaaag
    18421 gggatgtaaa atttagtgaa gctgttcaaa aggcggggtg gatcactcct gtccccgggg
    18481 gcgtaggtcc tatgacgatc gcaatgctgt taaaaaacac attacaggcg gcgcttttaa
    18541 atgaataggt ctgtcttttc cgtgggtcag attaataatt atgtaaaagg tctaatggac
    18601 agggatcccc tcctccaaaa tctatggata aggggggaaa tatcaaattt taaactgcat
    18661 tcctccgggc atatgtattt tacccttaaa gacgatcaat cacgaattag atgtgtgttt
    18721 tttaagtatc acaccacggg tttagccttc ataccatcag atggtatgag tgttattctt
    18781 ggggggaata tttccatata tacaagggat ggccaatatc aattttatgt tgagcatatg
    18841 gaaagggatg gtatagggcg attatatgca gcctttgaag tactgaaagc gaggctcagc
    18901 gaggagggtt tgttcagcga tgattttaaa aaacgactcc cgccctttcc caaaaagatt
    18961 ggcattgtca cctcccatac gggagccgcg gtccatgatg caataagggt tatcaccgaa
    19021 agaaataaaa cggtaagcat cctggtgata ccggtttcgg tgcagggtga gactgcaaag
    19081 gatcaaatca gcgctgctat agattatgca aatacaaggg atgatatcga tatcttaatc
    19141 gtcggtcggg gcggaggttc aatagaggaa ttatgggcgt ttaacgagga aaaggttgcc
    19201 aggactatct tccgctcaag aatccccata atatccgcag taggccatga aacggatttt
    19261 actatatcag atctggttgc agatgtaagg gctgcaaccc cttctgccgc tggggagata
    19321 gccaccagtg agctgacaaa catctgtcaa gccttggata ggttggatga ggggttgcaa
    19381 tactccatgt ccaaatatat ggctgaacag gatgctaaat tagtaagtct gtcggacagc
    19441 tatatgttaa aatacccaga acgttttttt tcttcctggg aacaaaggtt agactacaat
    19501 actgagaaaa tgaccggcct ggctgcctcc tatgcccaac agaagtatga tagttttatt
    19561 ggcatgatat cacaccttga ggccctaaac ccccttacga taatgatgag gggattttgt
    19621 attctcaccg atatgaacag gaaaactacg ataaactcaa tcgatcagct acatgaaggt
    19681 atgactgcga tgattcgctt gacagacggt atagccgctt gtaatataac taataaagaa
    19741 aaggagacgg gggatgaaaa caggagtcga taatggtatt tcttcactga aattcgaaga
    19801 tgccatgaaa gagttggaga taatcgtcga caggctggag ggaggggaat ctcccctaac
    19861 cctggaagaa tttgttgaac tatataaaag aggcgtgttc ttgtcccaac attgtaagaa
    19921 gacgctgcaa caggcgcaac aagaaattaa aatcttaaca aagaatttag atgggaatct
    19981 ggaagaggta ccattccagc aggaaaatca tgggggtgtt taatagacaa tacgatcgac
    20041 ttatgggact aataaccgcc tatctgaata gctccctgga gaaaatggaa agataccccg
    20101 aaacaatctt tgattctatg gagtatagcc tttttgcagg aggtaaacga ttacggccga
    20161 taatgttgtt ggcagttcat gagctgttgg gaggaaacat cgaagaatcc atgcccttag
    20221 cggggggaat ggaaatgatc cacacatact cccttataca cgatgattta ccggcaatgg
    20281 acaacgacga ttttaggagg ggaagaccca cgaatcataa gatatttgga gagggtatgg
    20341 caatactctc cggggatgcc ctcctaaact tagcctatga gaccatgttg gaaaatgccc
    20401 ttttatatcc ggacaggctc ataaatcata taagggcaat aaatgctata gcacgagcct
    20461 caggtattag gggtatggtt ggcgggcagg ttgtcgatct ggaaagtgaa gggaagcccg
    20521 ttaccgctga tactttaaaa tacattcacg atcataagac cggttcactc tttatagcct
    20581 caatttcggc cggcgtaatg ctagagaacc catcggaatc tgccgcacgg gctctcatag
    20641 aatatggaaa ttcacttggt ttagccttcc aggtagtaga tgatatctta gatgtgaccg
    20701 gtgaccgtca ggaaatgggg aaaaatatcg gtagggatat agataggggc aaggcgacct
    20761 atatagataa atatgggctg gaaaggtccc gatccatagt caagagtctc tatgaaagag
    20821 cattagacca gctggctata ttcggccctc ggggtgagtt tttaagtgaa ctggccggat
    20881 tcattattaa taggcataat taggaggaat cccatgagtt attacaccca gttgctttat
    20941 aataacattc tttgggtcag cctgctggca tggtttatag cacagttatt aaaggttgtt
    21001 tttactttga tctttaacaa aaagcttgac atcagaagat ttataggagc gggcggaatg
    21061 ccaagctccc attcagccct tgttgtatct ttagctacat ccgtgggaag gtttgaagga
    21121 tatgattccc ccatttttgc gcttgctcta accttttcgt taatagttat gtacgatgct
    21181 gccggtgtga gaagggcggc aggtaaacag gcggcggtac tcaacgaaat tttggaacaa
    21241 atccaaacaa gtaggagcat ctccgaggaa aaattaaagg aattgcttgg ccacactccg
    21301 attgaagtca tagct
//
LOCUS       DASRNX010000232         4326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_633866,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000232 DASRNX010000000
VERSION     DASRNX010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633866"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="VFD89_09220"
     CDS             complement(<1..1784)
                     /locus_tag="VFD89_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HZJ58397.1"
                     /translation="MIKFLKAKSLLFYFFISVLLCETVLKISTMQPFHRFDIFYVVMF
                     SFSFSIMFYLISGMFNGKDHFALAFVLLLLISIIFSSQMLYYKFFKTFYTIYSAGQGA
                     QLMGFWKDVLFVLSKNWLWIILFFLPPMVILIIKDKIIFCRRPGLGAFLLLLGLLILT
                     NVLAIGDIYLGDRQVNSHYELYFNPGNPLLTAQKLGLLTGMRLDAQRLIFTDGWNNPM
                     ANISDNKTKETYKPFEFEGDTGEPVETLNPENFYKKPTEDNPSPVYGPNILDIDFAEL
                     MASEKNNDIKDLHGYFLGVPATNKNEYTGKYKDYNLILVTAESFSPYAIHKDVTPTLY
                     KMVHEGYNFTNFYTPIWEVSTLDGEYVACTSLLPKRGVWSLYFSGRISMPFTMGNQLR
                     ALGYDTKAYHNHTYDYYRRDISHPNMGYDYKGIGNGLRLSESWPRSDLEMMEKTIPEY
                     LGNEPFHAYYMTVSGHMQYNFDGNHMAIKNKEYVEDLPYTEPGRAYIATQVEFDRAME
                     YLLKQLEEAGIAEKTLIAVSADHYPYGLEDNEIDDLAGHTLEKNFELYKSTFILYTKG
                     MEPVTIDTPASSLDIIPTLSNLLGLDYDS"
     gene            2049..3197
                     /gene="dgoD"
                     /locus_tag="VFD89_09225"
     CDS             2049..3197
                     /gene="dgoD"
                     /locus_tag="VFD89_09225"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HZJ58398.1"
                     /translation="MKIRQLELFKVPPRWLFLKVTTESGLVGWGEPVVEGKADSVAAA
                     VKEMEDYVIGRNAGDIEDIWQVLYRGAFYRGGPILTSAISGIEQALWDIKGKALNVPI
                     YELLGGAVRDKIRVYCWIGGDRPSNVAGAAQEKFDQGFTAIKMNATGELGWIDSFSKI
                     TEAAERVQAIRDKLGYKMDIAVDFHGRVHKGMAKVLMKELEPFKLMFIEEPVLTENEE
                     AFVELQRHTTTPIATGERNYTRWGFKTILSSGGVDIIQPDPSHAGGILETRKIAAMAE
                     AYDIAVALHCPLGPIALASCLQIDFCTPNAFIQEQSLGIHYNQGSDLLDYLEDPAVFK
                     YDKGYVLKQTKPGLGITINEEKVREMAKIGHTWKNPVWRDGDGVVAEW"
     gene            3209..3562
                     /locus_tag="VFD89_09230"
     CDS             3209..3562
                     /locus_tag="VFD89_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZJ58399.1"
                     /translation="MGIGKIGRPVKITSISFNNKGLGEISKIVDSEGAMGTNIIVLPE
                     TWRGIQLETIHGETIRTMSNLAKKHNTYIVCPIFREAHNKRYNSAILLGRDGNIICVY
                     DKVYPYHEEFNLDPP"
     gene            3613..4104
                     /locus_tag="VFD89_09235"
     CDS             3613..4104
                     /locus_tag="VFD89_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58400.1"
                     /translation="MAICFDANFPEVWKRLSDQGAELVIWVSAYSAGTSLLAHALNHN
                     YYIVSSTLVGDCIVYDISGKELLYEKSPEINISRVTLDLDRGIYHENYNLEKRDKLIE
                     DYEGAIEQDFHMEREEWFVLRAIKPGISARDTAKKYGMTELRDYRNLSRKRIDEMRGR
                     PIG"
BASE COUNT         1292 a          816 c         1065 g         1153 t
ORIGIN      
        1 gaatcgtaat cgaggcctaa gaggttagaa agggtgggta tgatatctag gctagaggcg
       61 ggcgtatcta tggtgacggg ctccataccc ttggtataga gtataaaggt actcttataa
      121 agctcaaaat tcttttcaag ggtatgtccg gccagatcat ctatttcgtt atcttcgaga
      181 ccgtaggggt aatgatcggc gcttacggct ataagggttt tttctgcgat tcctgcttct
      241 tccagctgct tgaggaggta ttccatggcc ctgtcaaatt caacctgggt ggcaatatac
      301 gccctcccgg gttcggtata cggaagatcc tccacatact ccttattttt tatggccatg
      361 tgattgccat cgaagttgta ctgcatatgg ccgcttacgg tcatatagta ggcgtgaaag
      421 ggctcattac ccaggtattc cggtatggtc ttttccatca tctcaaggtc ggaccttggc
      481 caggactcag agagtctaag gccgttacct atacccttat aatcataccc catatttggg
      541 tgtgagatat ctcgcctata ataatcatag gtatggttgt gataggcctt ggtgtcgtag
      601 cctaaagccc ttaattggtt gcccatggta aagggcatgg atatgcgacc tgaaaaataa
      661 aggctccaga cccctctttt aggtaaaagg cttgtacagg caacatattc accatctaag
      721 gtgcttacct cccatatagg ggtataaaag ttcgtgaaat tatagccctc gtgtaccatc
      781 ttatagaggg taggggttac atccttgtgt atcgcatagg gtgaaaaact ttcggctgta
      841 acgaggatga gattataatc cttatatttt cctgtatact cgtttttatt tgtggccggt
      901 acccctaaaa agtacccatg taaatcctta atgtcgttat ttttttcaga tgccataagc
      961 tcggcaaaat caatatccaa tatatttggg ccgtagacgg gacttggatt atcctctgta
     1021 ggttttttat aaaaattttc ggggttcaga gtctccacgg gttctccggt gtccccttca
     1081 aactcaaagg gcttataggt ctccttcgtt ttgttgtcac ttatattggc catgggatta
     1141 ttccatccgt ccgtaaagat tagcctttgg gcatccagac gcataccggt caaaagccct
     1201 agcttctgtg cagtcaatag gggatttccc ggattaaaat ataattcata atgggaattt
     1261 acctgtctat cccctaagta gatatcccca atggccaata cattggtgag gattaacaga
     1321 cccagcagca ggaggaatgc tccgagaccg ggtcgcctgc aaaatataat tttatccttt
     1381 ataatcaaaa ttaccattgg gggtaagaaa aacagaatga tccacagcca atttttggac
     1441 aggacaaaca atacatcctt ccagaatccc attagctggg ccccctgacc cgcagaatat
     1501 atggtgtaaa aggtcttaaa gaatttgtag tacaacattt gagaagaaaa gattatactg
     1561 attagaagga gcagcacaaa tgccaatgcg aaatggtcct ttccattaaa cataccggaa
     1621 attagataga acataataga aaatgagaaa ctgaacatga ctacatagaa tatatcgaac
     1681 ctatgaaagg gttgcattgt tgaaatcttt agaaccgttt cacacaaaag tacggaaata
     1741 aagaagtaga ataaaaggga ttttgcttta aggaatttga tcatgacgat atcgaccttc
     1801 cataaaagta tttaaatata agtatgctct ctgtcatccc attagggcat aggtaggtgc
     1861 gcacatatat acttaatcat gacagaactc agcaagcata tttatgtaaa tccccgaggt
     1921 tgagtttcgt tcattatatc attcacaaca ggtttatgca tgatataatg aaattgtatt
     1981 ggaaatttaa taattcctta tcaatataat aattggatag ctctataaaa tggaagggga
     2041 gaatagcgat gaaaataaga cagctggaac tatttaaggt gccaccgcgt tggctttttc
     2101 ttaaggtaac caccgaatcg ggattggtag gatggggaga gccggtagta gagggcaagg
     2161 ccgatagcgt tgccgcagcc gtaaaggaga tggaggacta tgtgattggc agaaacgctg
     2221 gggatatcga ggatatatgg caggtattgt ataggggcgc tttttatcgt ggcgggccca
     2281 tactcaccag cgccatatcc ggaatagagc aggccctgtg ggacataaag gggaaggcat
     2341 taaatgttcc catatacgaa ctcttggggg gagctgtcag ggacaagatc agggtatatt
     2401 gctggattgg cggagatagg ccttccaatg tagcaggggc agcccaggaa aaattcgatc
     2461 agggctttac cgcaataaag atgaacgcca ccggtgagct gggatggata gattcatttt
     2521 caaagattac agaggcggcg gaaagggtcc aggccattcg ggataagctg ggatacaaaa
     2581 tggatatcgc cgttgatttc catggaaggg tccataaggg gatggcaaag gttttaatga
     2641 aggaactgga gccctttaag cttatgttta ttgaagagcc tgtactaacg gaaaacgagg
     2701 aggcctttgt agagctccag aggcatacca ctaccccaat cgctacggga gaaagaaatt
     2761 ataccaggtg ggggtttaaa acgattctca gcagcggggg cgtggatatc atacagccgg
     2821 acccaagcca tgcaggggga attcttgaaa ccagaaagat cgccgctatg gcagaggcct
     2881 atgatattgc cgttgcgctc cattgtccct tagggcctat tgctttggct tcctgtctgc
     2941 agatcgactt ttgtactcca aacgctttta ttcaggagca aagtcttggc atccattata
     3001 atcagggctc agatctcctg gattatctag aagaccctgc cgtatttaag tatgacaagg
     3061 gctacgtact aaagcagacc aaaccgggcc ttggaataac aataaacgaa gaaaaggtca
     3121 gggagatggc caagatcgga catacgtgga aaaaccccgt ttggagggat ggggacggcg
     3181 ttgttgccga gtggtaaggg gggataaaat gggcataggt aagataggca ggccggtgaa
     3241 gataacgagc ataagcttta ataataaggg cctgggggag atatctaaga tagtggattc
     3301 agaaggtgcg atgggtacta atattatcgt actgcccgag acctggaggg gtatacaact
     3361 tgaaacaatc catggtgaaa caatcaggac aatgtccaac cttgcaaaga agcataatac
     3421 ctacatagtt tgcccaatct ttagggaagc ccataataaa cgctataatt ctgctatact
     3481 cttaggccgc gatggaaata ttatatgtgt ttatgataag gtctatccct atcacgagga
     3541 atttaattta gatcccccgt agaggtaggg acagaggttc ccgtagtcga tacggatttt
     3601 ggacgtatag gaatggccat atgttttgat gccaattttc ccgaagtctg gaagcgatta
     3661 tctgatcagg gggcagaatt ggtcatatgg gtaagcgcct attcagcggg cacttcatta
     3721 ctggcccatg cccttaacca taattattat atcgtatctt caaccctagt tggcgattgt
     3781 attgtctatg atatcagcgg aaaggaatta ttatacgaaa agagcccaga gatcaacata
     3841 agcagagtaa cgttggatct ggatcggggc atctatcacg aaaattataa tttggaaaaa
     3901 cgggataaat tgatcgaaga ttatgaaggg gccatagaac aggattttca tatggaacgt
     3961 gaagaatggt ttgttcttcg tgcgattaag ccgggcataa gcgcacggga tacagccaag
     4021 aaatacggta tgacagaatt aagggattac aggaacctga gcagaaaaag gatagacgaa
     4081 atgcgcggac ggcccattgg ctaaaaatac ctatagattt aagtgctaca aagtatgtta
     4141 taaaaacatt atgggtgtag gacttggggg gaagtgggca aatggattct ccgtgtcaaa
     4201 gaaactaaaa tggattgaca acagccacag ggcatcttat aattggactt gttgggggta
     4261 atagtagata tatttaatat caattttgct tgtctaatgg aaggggatta cagataagtt
     4321 atatac
//
LOCUS       DASRNX010000233         4714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_98274,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000233 DASRNX010000000
VERSION     DASRNX010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98274"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..885)
                     /locus_tag="VFD89_09240"
     CDS             complement(37..885)
                     /locus_tag="VFD89_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58401.1"
                     /translation="MIGVNDLVKSFGDKMALRGISFNVEDGEIFGFLGPNGAGKTTTL
                     RILTGQLKADSGKARIMELDATGGTREIHSRIGVVPEETNLYERLTIGQNLDLFCRLY
                     GCDIKQTDYYLERIGMAGEKNTQVKKLSKGMKQKVLLVRALLHSPKILFLDEPTSGLD
                     PASADTIHKVLKELNDQGITVLLTSHNMEEVDKLCNRVAFLNHGEIVEMGRPETLKMD
                     HSNRRMKVLIDDEHGLISKELDMDRAESAKLLSGWMTEGKVRSVHSCEPTLAEIFVKV
                     TGREFV"
     gene            1266..2684
                     /locus_tag="VFD89_09245"
     CDS             1266..2684
                     /locus_tag="VFD89_09245"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HZJ58402.1"
                     /translation="MRFGSDTEKKRLNGALIVGIGASMLGIAGTRLIARSVVNKVIDR
                     FYNIISKDLYDENLWELISSTIRMTPQVAMETNLRATEPKMLERPMGTPKKLPNLDSL
                     QFNVAQLHTMPTDTLTEIDTQVTIGKRASKPFTIKMPIMIAPMAYGVALSKKVKIALA
                     RGAKMAGTATNSGEGAFLPEERGEAGIFIYQYNRGHYGKTPEILRSCDAVEIQFGQGG
                     YGGAGHIVNDKLIDRQMRLDFGVPRGTDIVAHSRQPEVQSPEDLPALVEKLRDITEGV
                     PIGAKIGAGKYMEKDLAYLCESGVDFITVDGAEAATKGSPPIFQDDFGVPTVFAIHRA
                     AQWIKNNGYRDQVSLIVSGKIRTPGEVLKARALGADACYMGSIALFAISHSQLDKPLP
                     YEPPTTLVWYNAHYSRHFDIDKGAESLNNFLEACNLEIADGIRGLGKTSLAQVSKDDL
                     VSIDETVAKGCAIPMVYEPYVF"
     gene            2711..3751
                     /locus_tag="VFD89_09250"
     CDS             2711..3751
                     /locus_tag="VFD89_09250"
                     /inference="COORDINATES: protein motif:HMM:NF025644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HZJ58403.1"
                     /translation="MLANILIPDTWVSISERRNLAKPPTFSMKKLLSGEFFEGYEKYF
                     LDQFVSRDRLRGFAALVRSNIFGQRDNKGIYRVKDGIYKMEYPLNEGAVLHAANKFNE
                     IYDRYFQGRDVYYSVIPDKNYFMADKRGYLSLDYNLLLGILDDNLQHMNYIDIFRQLG
                     LKDYYRTDIHWDQNKIEHIADTLLKEMGGDPHMGDVQYNRRSLYPFYGSYYGQAVLKP
                     KPDTLIYLSDELIEDAVVYDHIDKTYNRIYVEDRFGSIDSYDLFLSGPKSMLTITNPE
                     ATVDKELIIFRDSFGSSIAPLMLKGYSRITLIDLRYLRSDLMENYMDIPQDPDVLFLY
                     NTAILNNSYMFK"
     gene            complement(3863..>4714)
                     /locus_tag="VFD89_09255"
     CDS             complement(3863..>4714)
                     /locus_tag="VFD89_09255"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZJ58404.1"
                     /translation="IMINNILVLKDGEEIAAYNWEPEIRQNQYSVSKSFTSSATGIAI
                     GEGLLTLDDPVIDFFPEKVPDNPPDRLRRLKVRHLLTMGVGHDESHLMAEQRYTTEET
                     DWVRLSLAIPIDYEPGARFVYSNVGPYLAGMIIRKLTGSDLRDYLMPRLFEPLGIWLP
                     TWETDPLGNSFGSGGLMLKVSEIAKLGQLYLQGGQWEGKQLIPKQWAEEVSKTQILTG
                     ADSPHNAGYSYLFWKGPEDSYRADGKYGQYSIIMPHKNAVVAINSYNTGDENILDYVW
                     EYLYPVI"
BASE COUNT         1231 a         1189 c         1051 g         1243 t
ORIGIN      
        1 ttatcaggat ggcgccaatc cttttaaatg ataaggtcat acgaattccc tccctgtcac
       61 cttaacgaat atttcagcca gtgtgggctc gcaggaatga acagaccgca ccttgccttc
      121 tgtcatccac ccggataaga gcttcgcgga ttctgccctg tccatgtcca attccttcga
      181 tatcagcccg tgctcgtcgt ctatcaagac cttcatacgt cggttggaat ggtccatctt
      241 tagggtctca ggtcttccca tctccacgat ttcaccgtgg tttaaaaatg ctaccctatt
      301 gcacagttta tcaacctctt ccatgttatg ggaggtaagc aagacggtta tcccttgatc
      361 gtttagctcc tttaggactt tatgtatggt atccgccgat gccgggtcga gtccggaggt
      421 aggctcgtct aaaaacagta tctttgggct atgtaaaagc gctctgacca gtaatacctt
      481 ttgcttcatt cccttggata gctttttgac ctgggtgttt ttctcccccg ccatacctat
      541 ccgttccaga tagtaatccg tttgtttgat atcacatccg tatagcctgc aaaaaagatc
      601 caagttttgt cctatggtca acctctcgta caggttggtt tcctccggaa ctacacctat
      661 gcggctgtga atttcccttg ttccccccgt ggcatcgagt tccatgatcc ttgccttccc
      721 gctgtcggcc ttcaactgac ccgtgagaat cctcagggta gtggtcttgc cggcgccatt
      781 gggccctaag aatccgaata tttcgccgtc ttccacatta aaggatatac ctcttagggc
      841 cattttgtca ccaaaacttt ttaccaaatc attaacccct atcaaaccaa tcacccctct
      901 tatccttaaa aatgttcaat taaatgtaaa atcattttcc gccatcgcac gcgcctaact
      961 ccaaaccacc ctgttctttt ccatcctgcg tatacttgga tcttactaca aacaataacc
     1021 aacggtttta ttatatagtt tttccccgat ctttccaacc tttttgttat atcccaaaaa
     1081 tcccaaatcg atatctaaat aggcgtttat tgacctccct gtgcaatatt gtcaggctcg
     1141 gagtgcggga acagatttat tccgtagccg acctgaatga taagcgttta tttccgcata
     1201 atatgattgt cgcccatatc ccctgcccat cattttacta tatagacatc agaggaggaa
     1261 atctcatgag atttggttca gacactgaaa agaaaaggtt aaacggagca ttgatagtcg
     1321 gaatcggcgc ttcgatgttg ggaatcgcag gcaccagatt aattgcccgt agtgtagtca
     1381 ataaggttat cgataggttc tacaatatta taagcaagga tttatacgat gagaacctat
     1441 gggaattgat ctcgtccacc ataaggatga ccccacaggt ggcaatggag accaatttac
     1501 gggctaccga gccgaagatg ctggagagac ccatgggcac tccgaaaaag ttgcccaatc
     1561 tggacagcct gcagttcaac gttgcacagc ttcataccat gcccaccgat acccttacgg
     1621 agatcgatac ccaggtaacc atcggcaaga gggcatccaa gccctttacc ataaaaatgc
     1681 ccattatgat tgctcccatg gcctacggtg tcgccctgag caaaaaggtt aaaatagccc
     1741 tagcccgcgg tgctaagatg gcaggaacgg ctaccaacag cggcgaaggg gcgtttctcc
     1801 cagaggaaag gggggaggcg ggaatcttta tttaccaata taaccgcgga cactatggta
     1861 agaccccgga aatcctccga agctgcgatg ccgtagaaat ccagtttggc cagggaggct
     1921 atgggggtgc cggtcatatc gtcaatgaca agctcatcga caggcaaatg cgtctggact
     1981 ttggcgtgcc ccgaggaacg gacatagtcg cccattccag acaaccggag gtgcagtctc
     2041 ccgaggatct gcctgccctg gtagaaaagc tgagggatat aaccgaaggc gtacccatag
     2101 gggccaagat tggcgcgggc aaatatatgg aaaaggatct ggcataccta tgcgagagcg
     2161 gcgtagattt tataaccgta gacggcgctg aagccgctac caaggggtct ccgccaatct
     2221 ttcaggacga tttcggagtc cccacggttt ttgccatcca tcgcgctgcc cagtggatta
     2281 agaataacgg ctaccgggat caggtaagcc taatagtatc gggcaagatc agaacccccg
     2341 gcgaagtatt aaaagcccga gccctaggtg ccgatgcatg ttatatgggc tccatagccc
     2401 tatttgctat atcccattcc cagctggata agcccttgcc ctatgaaccc ccaactacgc
     2461 tggtctggta taacgcccat tattcccgcc acttcgatat agataagggc gccgagagcc
     2521 tcaacaactt cctggaagca tgcaatcttg aaatcgctga cgggattagg ggacttggca
     2581 aaacatccct tgcccaggtg agcaaggatg accttgtaag tatcgatgaa acggtagcca
     2641 aaggatgcgc aatacccatg gtctatgagc cctatgtgtt ttgatgttta tggggataat
     2701 ctttggcttt atgctagcga atatcctgat cccggatact tgggtctcca tcagcgaacg
     2761 gcgtaacctg gccaaaccgc ctaccttctc catgaaaaag ctcctgagcg gcgaattctt
     2821 tgagggttat gagaagtatt tcctagacca gtttgtatcc cgtgacaggt taaggggatt
     2881 tgcggccctt gtaaggtcca atatctttgg gcaaagggat aataagggaa tatacagggt
     2941 gaaggacggt atttacaaga tggaataccc cttgaatgaa ggggctgtcc tgcacgcagc
     3001 aaacaaattc aatgaaattt acgacaggta ctttcaaggt agggatgtgt attacagcgt
     3061 tatacccgat aaaaactact tcatggcgga caaaaggggt tatctttccc tggattataa
     3121 tctattgtta gggattttag atgataatct tcaacatatg aattatatag acatattccg
     3181 gcaacttggg cttaaggact attataggac ggatattcac tgggatcaaa ataaaataga
     3241 gcatatcgca gatacgctct taaaagaaat gggaggggat ccccatatgg gggatgtaca
     3301 gtataataga aggtccctat acccctttta tggctcatat tacgggcagg cagtactaaa
     3361 acctaaaccc gataccttga tatacttatc cgatgaacta atagaggacg ccgtcgtata
     3421 cgaccatatc gataaaacct ataacaggat atatgttgaa gataggttcg gttccattga
     3481 ctcctatgac ctgttcttgt ccgggcccaa atccatgctt accattacaa accctgaggc
     3541 gacagtggat aaggagctta ttatctttcg tgattccttt ggcagcagca ttgcgcccct
     3601 tatgctaaaa ggatactcta ggattaccct tattgacctt cggtacctga ggtcggacct
     3661 gatggaaaat tatatggaca tcccccaaga ccctgatgtg ctcttcctgt ataacaccgc
     3721 gatccttaac aacagctata tgtttaaatg aaaattcatt taacctaaaa tccatccccc
     3781 ccagcaaaag ccctttggaa ccgggcttct aacttttagc ctccaacttc caagatccaa
     3841 gatcccaaat agctcattac cattaaatca caggatatag atactcccaa acatagtcta
     3901 agatgttctc atcccccgtg ttgtaggaat taatggcgac caccgcattc ttatggggca
     3961 taattatgct atattggcca tacttcccat cggcccggta ggaatcctcg gggcctttcc
     4021 aaaatagata gctgtatccg gcattatggg gggaatctgc tccggttagg atctgggttt
     4081 tggacacctc ttctgcccac tgtttgggga tcaattgttt tccctcccac tggccgcctt
     4141 gaaggtagag ctgccccagc ttggctattt cagacacttt aagcatcagc ccgcctgacc
     4201 cgaagctgtt tcctaaagga tcggtttccc aggtaggaag ccatatcccg aggggctcaa
     4261 agagcctggg catcagataa tcccgtaggt cagaacccgt cagcttcctg attatcatcc
     4321 ccgccagata aggtcccacg ttgctgtaga cgaagcgggc tccgggttca tagtctatgg
     4381 gaatagccag gctgagcctt acccaatctg tctcttccgt ggtatatctc tgctctgcca
     4441 tgaggtggga ttcatcgtgc cccacaccca tagtgaggag atgcctaacc tttaggcgcc
     4501 gcaacctatc cggcggatta tccggtacct tctccggaaa aaagtctata acggggtcat
     4561 ccaaggttaa taacccctct cctatggcaa tccccgtagc cgatgaagta aaacttttgc
     4621 tcacggagta ttgattttgt cgtatctcgg gttcccaatt atatgcggca atctcttccc
     4681 cgtctttgag aaccaggata ttattgatca tgat
//
LOCUS       DASRNX010000234         8994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_471701,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000234 DASRNX010000000
VERSION     DASRNX010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8994
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471701"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFD89_09260"
     CDS             <1..280
                     /locus_tag="VFD89_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HZJ58405.1"
                     /translation="GCCVEVPVLASKRGLDPIHVGPLPEHLAILNNINAGCEELAVEG
                     ALEGDKRKIFHAVCLDPLTSAVLSLQEIQDMVDEMFEANKDWLPHFDI"
     gene            545..1978
                     /gene="gnd"
                     /locus_tag="VFD89_09265"
     CDS             545..1978
                     /gene="gnd"
                     /locus_tag="VFD89_09265"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HZJ58406.1"
                     /translation="MHRVKSDIGLIGLAVMGQNLVLNMESKGYRVSVYNRTKKVTEGF
                     LEGTAKDRKNIIPSYTLEELIGSLKRPRRVMLMIKAGPPVDATIESLLPLLEEGDIII
                     DGGNSFFEDTVRRISNLKQHGIHYLGAGISGGEEGALEGPSIMPGGSKTAWESVEEIF
                     KDISAKVDNDIPCCDYIGPGGSGHYVKMVHNGIEYGDMQLISEAYYFMKEFLGMSATE
                     MQDVFVDWNDGELNSYLIEITSHILGRVDQETGRPLVDVILDRAGNKGTGKWTSQEAL
                     ELGTVAPTIVDAVFARYLSSVKEERMEASKVLEGPQPNFDATPAEKKKLIHDIGEALF
                     ASKICSYAQGFQLLKRASEAYGWELNLGEIALLWREGCIIRAQFLNKIKEAYDRNPDL
                     NNLMLDSYFRGILHRAQSGWRRIISLAVENGIPVSSFSSALAYFDGYRRETLSANMIQ
                     AQRDYFGAHTYERTDKEGTFHTEWLKD"
     gene            2031..3473
                     /locus_tag="VFD89_09270"
     CDS             2031..3473
                     /locus_tag="VFD89_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HZJ58407.1"
                     /translation="MNILALEASTSSAKAVLYSGTGGIIAARDQAYDLNISNVVTQDP
                     GGIYRALLDCGSSLIKSHGKDIKGDIDAIALCSTWHSLLFTDRRGEPVGRILTWANNN
                     AADIVSRYRGDTGLVNDIYKRTGCPVHTNFPLWKWLHFKEDNDIDTSSLRLSSQAEYI
                     FRRMTGDWGVSLSAASGSGFLNIHTLDWDEGILEFADLTIDQLAPLRDPEYTAPLRAE
                     AAKALCVREGIPVVITGPDGAFNQVGAGAMGDGIMTMSVGTSGAIRMATDSPILPEVP
                     STWCYYVAEGKRLAGAATSGAGNCVNWFVKTLNKNKYSYNALENFIDETRLMDAPTFL
                     PFMFGERSPGWEGSRLGGFTRLRGEHGLGELYYSVLEGILLNIYQCYEILTRVGGTPR
                     DIRISGGIENSEFWLQMAADIFQREIQTFDLEHASTMGAIAMALKALGGMGSLQEFKL
                     NPKKTIVPNKDKAEFYENRFQQYKEWYHKA"
     gene            3695..4339
                     /locus_tag="VFD89_09275"
     CDS             3695..4339
                     /locus_tag="VFD89_09275"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HZJ58408.1"
                     /translation="MDKEQIITKITQGGLVAVVRAESSEQAFRITEACIKGGVVGIEI
                     TFTVPGADKLIRDLSNVYNGGEIVIGAGTVMDPETARIAILAGAQYIVSPHFNPDTVK
                     LCNRYRIPSMPGATSIKEVVEAMEAGADIIKVFPGQILGPGVIQAILGPIPYAKLMPT
                     GGVDVDNVGDWIKAGAVAVGVGGSLTRGAKTGDYESITEVAKQFIANIKAARGI"
     gene            4445..5470
                     /locus_tag="VFD89_09280"
     CDS             4445..5470
                     /locus_tag="VFD89_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HZJ58409.1"
                     /translation="MPKRVVTFGEIMLRLSPPDHLRFIQADSFEVVYGGGEANVALSL
                     ANYGLDAAYVTKLPENSIGDAAINEMRRYGVDTRFIARGGERLGIYYYEKGASIRPSK
                     VIYDRAYSSIAEAKPGDIDWRAAFEGAEWFHFTGITPALGDSAAELTEEGCKIAKEMG
                     LTVSCDLNFRKKLWTSEKAGRVMGKLMEYVDIVVANEEDAEKVFGIKAADTDVTTGEL
                     DMSGYKYVAKEMMERFNLEVVTITLRESLSASDNNWSAMLYDGNEYLFSKKYELHIVD
                     RVGGGDSFGAGLIYSLMSGHGNRDALEFAVAASALKHTIIGDANHVSIDEVEALKGGD
                     ASGRVQR"
     gene            5670..6479
                     /locus_tag="VFD89_09285"
     CDS             5670..6479
                     /locus_tag="VFD89_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006778219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZJ58410.1"
                     /translation="MSTKRAFVTGGSRGIGRGIAWVLAKAGYDIAITYNTQEEEARSL
                     ASEIEDLGKKCFYYQASMEQVDVPEAITARAIEDLGGIDLLVCNAGLTRKHSLLTMTA
                     DEIDFLYGLNYRGYLLCSKVAANYMVDNKVKGNIIFIASTRGIRSYPDDSIYGSLKAA
                     LIRGTESFALEMSQYGIRVNCVAPGATAIRGDFTHEELSSSNLAKKIPAGRFGTPKEV
                     GYLVEFLASEKADYITGATIKIDGGLILPGMPEDNSEEAGYGWSRMRRTVW"
     gene            6495..7274
                     /locus_tag="VFD89_09290"
     CDS             6495..7274
                     /locus_tag="VFD89_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZJ58411.1"
                     /translation="MSDLLRRFKKALRDGECVIGPFMKTCDPAFVEVAGYAGFDFAIL
                     DMEHGPISVKEMENNIRGAIISNLVPIIRVPDSSETSIGKALDIGALGVQVPQIQNAQ
                     QARDVISKAKFSPKGERGLCRFVRAANYSALDRYEYFETANETMVILQLEGQEAMDNL
                     DEILEVEGIDILFIGPYDLSQSLGIPGDVTNPKVTEQMKYIVDKAKAKGIVVGTFIDE
                     PDSVDFWRQGGVQYFSYSVDVGIFLDACKTIVGYLKPKEEV"
     gene            7277..8479
                     /locus_tag="VFD89_09295"
     CDS             7277..8479
                     /locus_tag="VFD89_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZJ58412.1"
                     /translation="MAKITIENVKAILTAPAGINLIVVKVETSEPGLYGLGCATFAYR
                     EKLVASMVEDYLDPLLKGRDVSNIEDLWQLMNNNSYWRNGPVTNNAISGVDMALWDIK
                     GKLANMPLYDLLGGKSREGVAVYRHADGRDLETITENVQKFMDQNIRHIRIQWGGYGG
                     DAKTLNTPAGAPEGIYYCPDQYIRNTIKLFEHVRGQLGDEIELLHDVHERIPPIDTIR
                     LAKALEPYRLFFLEDVLSPEQGDWLRILRQQSCTPIAIGELYNNPKEWDYLIYNRLID
                     FIRVHISQIGGITPARKLGIFCEQFGIRTAWHGPGDVSPVGHAANIHLDLVSHNFGIQ
                     EWSGISDTLHEVFPGSPILKDGYVYANDKPGLGIDVNEELAAKYPCETRVTKWTQTRL
                     PDGTLHTP"
     gene            8519..>8994
                     /locus_tag="VFD89_09300"
     CDS             8519..>8994
                     /locus_tag="VFD89_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HZJ58413.1"
                     /translation="MKILVTMPKSRIRDSFFCPQVVNKLESIGEVGWNETSDNFSPEE
                     LRKKIRDVDVCMAGWGSARFDERVLENANRLKVIAYTGGSVANLVSEFLYEKGITVLS
                     GNEIYAESVAEGVLTYILASLRRIIFFQDQIAEGNWPAPEAWDEGILDQSIGLVGFG"
BASE COUNT         2552 a         1799 c         2484 g         2159 t
ORIGIN      
        1 gggatgttgt gtagaggtgc cggtactagc ctctaagcgt ggtttggatc ccatacacgt
       61 agggccgcta ccggaacatc tggccatctt aaacaatatc aatgccggtt gtgaagaatt
      121 ggctgtggaa ggagccctag aaggggataa gagaaagata ttccatgcag tttgcttgga
      181 tcctttgaca tcggcggtat tgagcctgca ggagatccaa gatatggtgg atgagatgtt
      241 tgaagccaac aaggattggt tgcctcattt tgacatttaa ggcagtatag cggggtttgt
      301 cgccaagcct ctaaagggac ggcgatggaa ttaattttga ttagagtttt ttacaaaata
      361 tccggattct aattaaagga cacgaaatgt gggtataaat aggggtcaag gggtctaagg
      421 ggttcctttg atccctgttt ttttgtatag ggcccggcgg actttatatt tgtcagtaca
      481 tatcccccat gaagtatggt attattatcg tagggattta cgacttattg cactttaaag
      541 gaggatgcac agagtgaaat cggatatcgg cttgatagga ctggcggtga tgggccagaa
      601 tttggttctc aacatggaga gcaaaggata cagggtttca gtctataacc ggaccaagaa
      661 ggttaccgaa gggttccttg aagggacggc taaggatagg aaaaatatta ttccctcgta
      721 tacattggaa gaattgatag gatctttgaa aaggcccagg agggtgatgc ttatgataaa
      781 ggcaggccca ccggtagatg ccaccattga gagcctattg ccattattgg aggaagggga
      841 tataataata gatggaggta attccttttt tgaagatacc gtaagaagga tttcgaattt
      901 aaagcagcac ggaattcatt acctaggtgc cggcatttcc gggggcgaag agggtgcctt
      961 agagggtccc agcattatgc ccgggggaag caaaacagcc tgggaaagtg tagaagagat
     1021 atttaaggac atctcagcaa aggtggacaa tgatataccg tgttgtgatt atatcggtcc
     1081 cgggggatcc ggtcattacg taaagatggt ccacaacggc atcgaatatg gggatatgca
     1141 gcttattagc gaggcctatt actttatgaa ggaatttctc ggcatgtcgg ccaccgagat
     1201 gcaggatgta tttgtggatt ggaacgacgg tgagttaaac tcttacctga ttgagataac
     1261 ctcccatata ttggggagag tggaccaaga aaccggcagg cccttagtgg atgttatcct
     1321 ggatagggca ggtaataagg ggacgggtaa atggacatcc caggaggcac tggaattggg
     1381 gaccgtggcg cctaccatag tagatgccgt gtttgcaaga tatctgtcgt cagttaaaga
     1441 ggagcggatg gaggcctcca aggtattaga gggtccgcaa ccgaattttg atgctacacc
     1501 cgctgaaaag aaaaaactta tacacgatat aggcgaagcc ttatttgcgt ctaagatatg
     1561 ttcctatgcc caggggtttc aattgcttaa aagggcatcg gaagcctatg ggtgggagct
     1621 aaacctaggg gaaattgccc tgttatggag ggaagggtgt atcatacggg cccagtttct
     1681 aaacaagata aaagaggcct acgataggaa cccggacctt aataacctta tgctggattc
     1741 ctatttccgc gggattttac atagggctca aagtgggtgg agacggatta tcagcctggc
     1801 ggttgagaat ggtataccgg tatcgtcctt cagttcggca ttggcatatt ttgacggata
     1861 cagaagggag accctgtccg ccaatatgat tcaggcacag agggattatt tcggggccca
     1921 tacctatgag cgcacagaca aggaaggcac tttccataca gaatggttaa aggactagaa
     1981 gaacaatttt tttgatcgat acatctgaaa ctttctaaag gggtgtaagg ttgaatattc
     2041 tggcattaga agctagcact tcatcagcga aggctgtatt atattcgggt accgggggca
     2101 ttatagccgc ccgggaccag gcctatgact taaatattag caacgtagta acccaggatc
     2161 caggcggtat ctatagggcg ctgctggatt gcggcagttc cttaattaaa tcccatggaa
     2221 aggatataaa aggggacatt gacgccatag ccctttgcag tacatggcat agcctattgt
     2281 tcacagatag gcggggtgag cctgtgggca gaattctcac ttgggctaac aacaatgcag
     2341 cggatattgt tagccggtac aggggggata caggattagt aaatgacatc tacaagagga
     2401 ccggctgtcc tgttcatacc aactttcccc tttggaaatg gttgcatttc aaggaagata
     2461 acgatataga tacaagctct ctgcgcctgt cgtcccaggc tgaatatatt ttcaggcgca
     2521 tgaccgggga ttggggagta tccctcagcg ccgcatcagg cagcgggttt ttaaatatcc
     2581 ataccttaga ttgggacgaa gggatattgg agtttgcaga tctcaccata gaccaattag
     2641 cgcccttgcg ggatccggaa tataccgccc ctttaagggc cgaagcagcc aaagccttat
     2701 gcgtaaggga agggatcccc gtagttatta caggccctga cggggccttt aatcaggttg
     2761 gtgcgggagc tatgggcgat ggtattatga ccatgtccgt cggtaccagc ggagccattc
     2821 gaatggctac ggacagccct atattgcccg aagtgccgtc aacctggtgc tactatgttg
     2881 cagaggggaa aagacttgcc ggagcggcta cgtccggggc aggcaattgt gttaactggt
     2941 ttgtaaaaac cttaaacaaa aacaaatata gctataatgc cttggagaac tttatagacg
     3001 agacaaggct tatggatgca ccgacatttc tcccctttat gtttggggaa cgttccccgg
     3061 gctgggaggg ttctaggctg ggcggattta cgcgactcag gggcgaacac gggttagggg
     3121 agctttacta ttccgtcttg gaagggattc tgctaaacat ctatcaatgc tatgagatac
     3181 ttacccgagt gggcggaacc ccccgggata taaggatttc tgggggcatt gagaattctg
     3241 aattctggct ccagatggcg gctgatattt tccaaaggga gatacagacc tttgacttag
     3301 agcatgcgtc caccatgggc gccatagcca tggctctcaa ggccctgggg ggcatgggtt
     3361 ccctgcagga attcaaactg aaccctaaga agaccatagt tcccaacaag gataaggccg
     3421 aattctatga aaatagattc caacaatata aggaatggta ccataaggct taaagagctg
     3481 tttatttcaa gtacccaata tgctttaaag ccgccgggaa tgattcatag caatggaata
     3541 aacccgggtg ctttatacag ataagcaata cggaaactaa atttatgggc atgataattg
     3601 taatggtttt atgcaatttc atgatgataa atttcatagt cggagttgtt tatctaaata
     3661 gataaatata tattaaggga aaggggtagc gaaaatggat aaggaacaaa ttataaccaa
     3721 gataacccag ggcggtttag tggcggtagt aagggccgaa tccagcgagc aagcctttag
     3781 gattaccgag gcatgtatca aaggcggggt agttggaatt gaaattacct ttaccgtacc
     3841 cggggcagac aagcttataa gggatctatc gaacgtttac aacggcggag agatcgtaat
     3901 aggtgcggga acggtaatgg acccggagac ggccagaata gctatcctgg caggggccca
     3961 gtatatagtc agtccacatt tcaatccgga taccgtaaag ctttgtaata ggtatcgcat
     4021 tcccagtatg ccgggggcta cgtccatcaa ggaagtggta gaggctatgg aagcgggggc
     4081 ggatataatt aaggtattcc ccggtcagat tttagggcca ggggtcatcc aagccatttt
     4141 agggcctatc ccctatgcaa agctgatgcc taccggcggg gtagacgtgg ataatgtcgg
     4201 cgattggatc aaagctggtg cagtggcggt gggtgtgggc ggttccttga cacggggtgc
     4261 caagaccggc gattatgagt ccattaccga ggtagccaaa caatttatag caaatataaa
     4321 agccgcaagg ggaatatgat cgcaaaaaca cattgtttat ctcaattacc ggtagtaaga
     4381 agacgaagaa cttacgggac aaagtctaca aatcatattt ttaaaataaa agagggggat
     4441 ataaatgcct aagagagttg ttacttttgg agaaattatg ctgagactgt caccaccgga
     4501 tcatctgcgt tttatacaag cagatagctt tgaagttgtc tacggaggcg gtgaagctaa
     4561 tgtagctcta tcccttgcca attatggact ggatgcggct tatgttacga agcttcccga
     4621 aaactccatc ggagatgcag ctatcaatga gatgagaaga tatggggtag ataccaggtt
     4681 tattgccaga ggcggtgaac gattggggat ctattattat gaaaagggtg cctcgatcag
     4741 accgtctaag gtgatatacg acagggccta ttcctctatc gccgaggcaa agcccgggga
     4801 tatagattgg agagcggcct ttgagggggc ggaatggttc catttcaccg ggattacccc
     4861 tgctcttgga gatagtgcag ccgaattgac agaagagggc tgtaagatag caaaggagat
     4921 ggggcttacc gtcagctgtg acctgaattt caggaaaaag ctatggacct cggaaaaggc
     4981 cggcagggta atgggcaagc taatggaata tgtagacatc gtcgtggcca acgaagagga
     5041 cgctgaaaag gtatttggaa taaaagccgc agataccgac gttaccaccg gagaattgga
     5101 tatgtcgggg tacaaatacg tcgcaaaaga gatgatggag cgctttaacc ttgaggttgt
     5161 taccattacc ttgagggaga gcctttcggc ttcggacaac aactggtcag ctatgctcta
     5221 tgacggaaac gaatatctgt tctcaaagaa atatgagttg cacattgtag acagggttgg
     5281 gggtggagac tcctttggag cagggcttat ctactcgcta atgtccggac acggaaacag
     5341 ggatgcgtta gaatttgcgg ttgctgcttc ggcactaaag cataccatta tcggggatgc
     5401 aaaccatgtc tccatagacg aggtcgaggc attaaagggc ggcgatgcgt cgggtagggt
     5461 acaaaggtaa attttaatac agaaatctga ttttccatat taaaagggtc gggggctgtt
     5521 cgtcgccatt ataggcggcg gatatacccc cttaccctat tagaacctgt ctgttacaat
     5581 tatcatagcc gtcgtattat gcagatataa ataaatattg cgcatttcat caaaatgtta
     5641 tctgaaagta ttgagaggag gacatcaaaa tgtcaacaaa aagggcgttc gtaaccggcg
     5701 gaagcagggg aatcggcagg ggtattgcat gggttctggc aaaggcaggt tatgatatag
     5761 ctataaccta taatactcag gaggaagagg cccggtcact ggcatcggag atagaggacc
     5821 tggggaaaaa gtgtttttat tatcaggcaa gcatggaaca ggtcgatgta cctgaggcca
     5881 taacagcacg ggccatagag gaccttggag gaattgatct cttggtctgc aatgccggtt
     5941 tgaccagaaa gcatagcctg ctgactatga ccgccgatga aatagacttt ttgtacgggc
     6001 tcaattacag aggatatctg ttgtgctcca aagtcgccgc taattatatg gtggacaaca
     6061 aggtcaaggg aaacatcatc tttatcgcat ccacaagggg catacggtcc tatcccgatg
     6121 attccatcta cggatcctta aaggcggccc ttatccgcgg caccgaatcc tttgccctcg
     6181 agatgtccca atacgggata agggtaaact gcgtcgcacc gggcgctacc gctatccggg
     6241 gagacttcac ccatgaggag ctgagctcca gcaatctggc caaaaagata cctgcaggca
     6301 ggtttgggac cccaaaagaa gttggatacc tggttgagtt tttagcatct gaaaaggcgg
     6361 actacataac cggcgcaacc attaaaatag acggagggct tatattgcct ggaatgcccg
     6421 aggacaattc cgaggaagca ggctacggct ggtccaggat gagaagaacc gtttggtaga
     6481 aagggggcaa agaaatgagt gacttactga gacgattcaa gaaggctttg agggacgggg
     6541 aatgtgttat tggccccttt atgaaaactt gtgaccctgc tttcgtggaa gtagcgggtt
     6601 acgccgggtt tgattttgct atcttggata tggaacacgg ccccatatcc gttaaggaga
     6661 tggaaaacaa tattcgaggc gccataatat ccaatcttgt tcccataata agggtaccgg
     6721 attccagcga gaccagcatc ggaaaagccc tggatattgg agcgttgggg gttcaagtgc
     6781 cacagataca aaatgcccag caggcaaggg atgttatatc gaaggctaaa ttttccccga
     6841 aaggcgaacg gggtctgtgc cgctttgtca gggcagcgaa ttattcggcc ctggaccgat
     6901 atgagtactt cgaaactgca aacgaaacca tggttatcct acagttagaa gggcaggagg
     6961 ccatggataa cttagacgag attttagagg tggagggcat agacattcta tttatcgggc
     7021 cctatgacct gtcccaatcc ctgggaatcc ccggcgatgt tacaaacccc aaggtaacag
     7081 agcagatgaa gtacatcgtg gataaagcca aggccaaggg tatagtggtg ggaaccttta
     7141 tcgacgaacc ggattccgtg gatttctgga gacagggcgg ggtacaatac ttctcctatt
     7201 cggtagatgt aggaatattt ttagatgcat gcaaaacaat tgtaggatac ttgaaaccta
     7261 aagaggaggt ataggtgtgg ctaaaataac aatagagaat gtaaaagcga ttctgacggc
     7321 gcctgcgggg atcaacctga tagttgtgaa ggtggaaacc agcgagccgg gactgtatgg
     7381 cctgggatgt gctacctttg cctataggga aaagctggtt gcatccatgg tagaggatta
     7441 tttagatcca ctattaaagg gcagggacgt ctccaacata gaggatctat ggcagctgat
     7501 gaacaacaac tcgtattgga gaaacgggcc ggtaaccaac aatgcaattt caggggttga
     7561 tatggctctc tgggatatca agggtaaatt ggccaatatg cccctctacg acttgttagg
     7621 gggcaaatcc agggaaggcg ttgcagtcta tcgccatgca gacggcaggg atcttgaaac
     7681 catcaccgag aacgtccaaa agttcatgga ccaaaatatc cggcatataa ggatacaatg
     7741 gggcggatac ggcggggatg ccaaaaccct caacacaccg gcaggtgccc cggagggaat
     7801 atactattgt cccgaccagt acataagaaa taccataaaa ctctttgagc acgttcgggg
     7861 acagctggga gacgaaatcg agctattgca cgacgttcat gagaggatcc cgcccatcga
     7921 caccatcagg cttgctaagg ccctggaacc ttataggctg ttcttcttag aggacgttct
     7981 atcaccggag cagggggact ggctcaggat attacgccaa caaagctgca cccccatcgc
     8041 aataggggaa ttatataata acccaaagga atgggattat ttaatataca ataggctgat
     8101 agattttatc agggttcata taagccagat aggcggaata accccggcca gaaagcttgg
     8161 catcttctgt gagcaattcg gaataagaac cgcttggcac ggccccgggg atgtgtcgcc
     8221 ggtaggtcat gcagccaaca tccatttaga cctggtatcc cataactttg gaatccaaga
     8281 gtggtctggt atatccgaca ccctccacga agttttcccc ggaagtccaa tccttaagga
     8341 cggatatgta tatgccaacg ataagccggg attgggaatc gatgtgaacg aggaattagc
     8401 ggcgaagtat ccatgcgaaa caagggtcac caagtggact cagacgagat tgcccgacgg
     8461 aacgctccat actccttaat attaaatatt tagtattaaa tctttggagg cgaggattat
     8521 gaagatcttg gtcaccatgc caaaatccag gattagggac agcttttttt gtccccaggt
     8581 cgtcaataaa ttagaatcaa ttggagaagt cgggtggaat gagacttcgg ataatttcag
     8641 ccccgaggaa ttaaggaaaa aaattagaga tgtagacgtt tgcatggccg ggtggggcag
     8701 cgccagattt gatgagagag tgctagaaaa cgccaatagg ctcaaggtca tcgcttatac
     8761 aggcggctcc gtagccaatt tagtgagcga atttctctat gaaaagggta ttaccgtact
     8821 cagcggaaat gaaatctatg cagagtcggt ggccgagggc gttcttacat acatcctcgc
     8881 ctctttgaga cgaataatat tctttcagga tcagatagct gaggggaatt ggcccgctcc
     8941 ggaagcttgg gacgagggga ttttagacca aagtatcggg cttgtgggat ttgg
//
LOCUS       DASRNX010000235        12469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_471837,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000235 DASRNX010000000
VERSION     DASRNX010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471837"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /gene="tuf"
                     /locus_tag="VFD89_09305"
     CDS             <1..134
                     /gene="tuf"
                     /locus_tag="VFD89_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805135.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZJ58414.1"
                     /translation="MPGDNIDMEVELITPIAIEEGLRFAIREGGRTVGAGVVASVAK"
     gene            321..629
                     /gene="rpsJ"
                     /locus_tag="VFD89_09310"
     CDS             321..629
                     /gene="rpsJ"
                     /locus_tag="VFD89_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZJ58415.1"
                     /translation="MASQKIRIKLKAYDHSLIDQSAEKIVGAAKKTGAKVSGPIPLPT
                     ERDIITILRSPHKHKDSREQFEMRTHKRLIDILSPTPKTVDSLMRLDLPAGVDIEIKL
                     "
     gene            754..1383
                     /gene="rplC"
                     /locus_tag="VFD89_09315"
     CDS             754..1383
                     /gene="rplC"
                     /locus_tag="VFD89_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZJ58416.1"
                     /translation="MKKGILGKKLGMTQIFTEDGTVIPVTVVEAGPCVVTQIKTQDKD
                     GYDAVQVGYGDIREKLLNRPQKGQFDKTGISYRRHIREFPFNNTEDYKIGQEIKADVF
                     TVGDRVDVAGRSKGKGFAGNIKRWGHGKGPMSHGSHYHRGPGAMSAGSSPAKVLKNKK
                     LPGRMGNVRTTVQNLEVVRVDTERNLLMVKGAIPGARGNMLIIKETVKR"
     gene            1415..2038
                     /gene="rplD"
                     /locus_tag="VFD89_09320"
     CDS             1415..2038
                     /gene="rplD"
                     /locus_tag="VFD89_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZJ58417.1"
                     /translation="MPKVAVYNIEGASVDEIELDDNIFGIKINEALLHQVVRMQLNNK
                     RQGTQSTQTRAEVRGGGAKPWRQKGTGRARHGSIRSPIWVGGGVTFAPKPRDYSFRMP
                     KKMRRLALKSALSSKVENSQIVVLDDLKLAQPKTKEMVKVLKNLEVNGKALLVLPEND
                     ETVARASGNIQGVKLASVNTLNVLDILNYDKFIITKEAALKVEEVYA"
     gene            2035..2331
                     /gene="rplW"
                     /locus_tag="VFD89_09325"
     CDS             2035..2331
                     /gene="rplW"
                     /locus_tag="VFD89_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZJ58418.1"
                     /translation="MMKNPHDIILRPVLTEKSYDQIADRKYTFLVDVRANKTEIKKAA
                     EEIFGVKVEGVNTLKQLGKLRRQGVHIGRRPTTKKAIIKLTPDSKTIEFFEGMA"
     gene            2356..3186
                     /gene="rplB"
                     /locus_tag="VFD89_09330"
     CDS             2356..3186
                     /gene="rplB"
                     /locus_tag="VFD89_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZJ58419.1"
                     /translation="MAIKKYNPTSPAKRHMTVSSFEEVTKFTPEKSLLDTLKSKAGRN
                     SDGRITVRHRGGGAKRKYRIIDFKRDKDGVPAKVAGIEYDPNRSANIALLHYVDGEKR
                     YIIAPNGLKDGDMVVSGPDADIKVGNALPLQNIPLGTVIHNIELRVGSGGQLVRSAGN
                     SAQLMAKEGKYGHVRLPSGEVRLVLLTCRATIGQVGNIDHENIAVGKAGRKRHMGFRP
                     SVRGSAMNPVDHPHGGGEGRSPVGRPSPVTPWGKPTLGFKTRQKSKKSDKYIIRKRKG
                     "
     gene            3261..3545
                     /gene="rpsS"
                     /locus_tag="VFD89_09335"
     CDS             3261..3545
                     /gene="rpsS"
                     /locus_tag="VFD89_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZJ58420.1"
                     /translation="MSRSIKKGPFVSESLMKKIIGMNKSGKKTVIRTWSRASTIFPEM
                     VGHTIAVHDGRKHVPIYITEEMVGHKLGEFAPTRTFRGHSGHTARSTSLK"
     gene            3569..3955
                     /gene="rplV"
                     /locus_tag="VFD89_09340"
     CDS             3569..3955
                     /gene="rplV"
                     /locus_tag="VFD89_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZJ58421.1"
                     /translation="MALRVKEKALKRRENRDQRPKATAKHIRMSSRKVKAVIDLIRDK
                     NIDEAQAILLNTPRAATQPVLKVLNSAAANAENNLGMDRDNLYVAEVYANQGPTLKRF
                     RPRAHGRAARIRKRTSHITIILDEKN"
     gene            3973..4653
                     /gene="rpsC"
                     /locus_tag="VFD89_09345"
     CDS             3973..4653
                     /gene="rpsC"
                     /locus_tag="VFD89_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZJ58422.1"
                     /translation="MGQKINPHGLRVGVIKDWDAKWYASKKDFADTLLEDHGIRELLK
                     KKLYAAGISKIEIERAANRVKVNIYTAKPGIVIGRGGSGVDALKVELEKFTGKTVIIN
                     IVEVKNADMDAQLVAENIASQLERRISYRRAMKQSMARAMRAGAKGIRTMVAGRLNGA
                     EIARSERYHEGSIPLQTLRADIDYGFTEANTTYGKLGVKVWIYKGEILPGAKENPRTE
                     GGNRRVNA"
     gene            4640..5077
                     /gene="rplP"
                     /locus_tag="VFD89_09350"
     CDS             4640..5077
                     /gene="rplP"
                     /locus_tag="VFD89_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZJ58423.1"
                     /translation="MLMPKRVKRRRVHRGRMKGKANRGNTITYGEFGLQAIEPGWITS
                     NQIEAARVAMTRYIRRGGKVWIKIFPHKPVTAKPAETRMGSGKGAPEYWVAVVKPGRV
                     MFEIAGVSEEIAREALRLASHKLPLKAKFIQREEMGGESSESQ"
     gene            5064..5267
                     /gene="rpmC"
                     /locus_tag="VFD89_09355"
     CDS             5064..5267
                     /gene="rpmC"
                     /locus_tag="VFD89_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZJ58424.1"
                     /translation="MKANRFRELSDEELQDRVAEFKSELFNLRFQLATGQLENPMRIK
                     EVRKDIARVKTIIRQRELNSARI"
     gene            5288..5551
                     /gene="rpsQ"
                     /locus_tag="VFD89_09360"
     CDS             5288..5551
                     /gene="rpsQ"
                     /locus_tag="VFD89_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZJ58425.1"
                     /translation="MAEDARAIRKARTGVVVSNKMDKTIVVAVENRTKHKLYGKIIKR
                     TMKLKAHDEGNECQIGDRVRVMETRPLSKDKHWRLVEIIEKAQ"
     gene            5586..5954
                     /gene="rplN"
                     /locus_tag="VFD89_09365"
     CDS             5586..5954
                     /gene="rplN"
                     /locus_tag="VFD89_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZJ58426.1"
                     /translation="MIQQESRLKVADNTGAKEVLCIRVLGGTRRRYANIGDVVVATVK
                     SATPGGVVKKGEIVRAVIVRSKKGIQRNDGTSIRFDDNAAVIINDARQPRGTRIFGPV
                     ARELREKDYMRIISLAPEVL"
     gene            5975..6310
                     /gene="rplX"
                     /locus_tag="VFD89_09370"
     CDS             5975..6310
                     /gene="rplX"
                     /locus_tag="VFD89_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZJ58427.1"
                     /translation="MAVPKRKLHVKGGDTVEIISGKDKGKRGKVLKTLPKEGKIIVEK
                     INMLTVHTKPRGMEQPGGIIHREGPIYASKAMPVCERCNKKTRIGRRVLEDGSKVRVC
                     RVCGETFND"
     gene            6336..6875
                     /gene="rplE"
                     /locus_tag="VFD89_09375"
     CDS             6336..6875
                     /gene="rplE"
                     /locus_tag="VFD89_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZJ58428.1"
                     /translation="MARLKELYEKEIVPALMERFNYKNVMEAPKLDKIVINMGVGDAK
                     ENPKFLESAVNDLSIIAGQKPIVTAAKKSVSNFKLREGMRIGTKVTLRGDRMYEFADR
                     LLNVALPRVRDFRGVSARSFDGRGNYSLGIREQLIFPEINYDQIEKVRGMDIVFVTTA
                     KTDEESREFLRLLGMPFAN"
     gene            6895..7080
                     /locus_tag="VFD89_09380"
     CDS             6895..7080
                     /locus_tag="VFD89_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZJ58429.1"
                     /translation="MAKKSWRVKQQREPKFSTRAYNRCRICGRSHAYLRKFGLCRICF
                     RELAYKGQIPGVRKASW"
     gene            7110..7508
                     /gene="rpsH"
                     /locus_tag="VFD89_09385"
     CDS             7110..7508
                     /gene="rpsH"
                     /locus_tag="VFD89_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZJ58430.1"
                     /translation="MVMTDPIADMLTRIRNALVVKHASINVPASNMKMAIANILREEG
                     FIRGFTRMEDGKQGVIRIDLKYGQNGQKVIAGLKRISKPGLRVYANKDQVPKVLGGLG
                     VAILSTSKGVITDKKAREEGVGGEVLCFIW"
     gene            7579..8118
                     /gene="rplF"
                     /locus_tag="VFD89_09390"
     CDS             7579..8118
                     /gene="rplF"
                     /locus_tag="VFD89_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZJ58431.1"
                     /translation="MSRIGNLPIDIPQGVTVTVDGNNLVTVKGAKGELSQPINREIAV
                     TVEDNVVILKRSSDAKFQRSIHGLSRSLVANMVEGVSKGFQKNLEINGVGYRAQKQGK
                     KLILAVGYSHPVEIEEADGVEFEVPAANRIIVRGIDKQKVGAMAAKVRDTRPPEPYKG
                     KGIKYENERIIRKEGKTGA"
     gene            8140..8505
                     /gene="rplR"
                     /locus_tag="VFD89_09395"
     CDS             8140..8505
                     /gene="rplR"
                     /locus_tag="VFD89_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZJ58432.1"
                     /translation="MIKKIDKNAARKVRHLRVRNRVSGSVEIPRLNVFRSLKHIYVQL
                     IDDNGGHTLVSASSLDPEIKSVSDKTRKEVAKMVGELAARRALEKGIKKVVFDRGGYI
                     YHGRVSEVAAGAREAGLEF"
     gene            8518..9018
                     /gene="rpsE"
                     /locus_tag="VFD89_09400"
     CDS             8518..9018
                     /gene="rpsE"
                     /locus_tag="VFD89_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZJ58433.1"
                     /translation="MEQIDASTLDLKEKVVTINRVAKVVRGGRNFRFNTVVVVGDENG
                     HVGVGTGKATEIPEAIRKGIEDAKKHIMKVPIVNTTIPHEVNGRFGSGSVLMMPAKEG
                     TGVIAGGPVRAVLELAGIRDILTKSLGSSNPRNMVNAAMEGLSRLKTVEDVARLRGKA
                     VEEILG"
     gene            9032..9217
                     /gene="rpmD"
                     /locus_tag="VFD89_09405"
     CDS             9032..9217
                     /gene="rpmD"
                     /locus_tag="VFD89_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZJ58434.1"
                     /translation="MAKLKVTQVRSTIGSKKDQIATMEALGLRKIRSEKIHDDSPTIR
                     GMIDKVKHLVSVEEIDA"
     gene            9232..9672
                     /gene="rplO"
                     /locus_tag="VFD89_09410"
     CDS             9232..9672
                     /gene="rplO"
                     /locus_tag="VFD89_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZJ58435.1"
                     /translation="MRLNRLSPAEGSTKTSKRKGRGPGSGLGKTSGRGHKGQKARSGG
                     GVRPGFEGGQMPLTRRLPKRGFTNIFAKVYNEVNLVQLDVFEQDTIVTPELLKKEGII
                     SKEKDGVKILGNGELNIKLIVKAHKFSKAAKEKIEAAGGKAEVI"
     gene            9674..10948
                     /gene="secY"
                     /locus_tag="VFD89_09415"
     CDS             9674..10948
                     /gene="secY"
                     /locus_tag="VFD89_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZJ58436.1"
                     /translation="MLDTLRNAWRIEDIRKKIVFTMIMILVYRVGSFIPVPFIDASHI
                     KDLVGEGNLLGLLDIISGGAFANYTIFAMGITPYINSSIIMQLLTVAIPKLEQLSKEG
                     EEGQKKITQYTRYLTIGLAFVQSAGITYGLGNQAGGQIMTNHSVWAFFVVAMTLTAGT
                     AFLMWLGEQITENGIGNGISMIIFASIVSRIPVAAVSLWNLAFEVGTISPWSLPLIAI
                     FIVALIAGVVFIDMGQRRIPVQYAKRVVGRKVYGGQSTHIPMKVNSSGVLPIIFAVSL
                     LSLPQTIGYFFPEGGFFRFIEANFSQKSAAYAIVYALLIVFFTFFYSQISFNPIEVAN
                     NLRQYGGFVQGIRPGKPTSDYLLRISNRITLVGSLFLAAVAVIPIAVAGFTKIPMYLQ
                     GTSILILVSVALETSNQLEAQMVMRHYKGFLK"
     gene            10959..11708
                     /gene="map"
                     /locus_tag="VFD89_09420"
     CDS             10959..11708
                     /gene="map"
                     /locus_tag="VFD89_09420"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZJ58437.1"
                     /translation="MIILKSKSEIKLMEEAGRIVAGAHEVVKNAIAPGITTAELDEIA
                     EDYIRSRGGIPAFLGYQGYPASICASINEQVVHGIPGPVALKEGDILSVDIGAFHNGY
                     CGDAARTHPVGEVTPDAHRLIETAKEAFFSGLEFAREGYRLSDISHAIQVYVEGKGYS
                     VVKALVGHGIGQAMHEEPQIPNFGPPGRGPRLRKGMALAIEPMINAGAHEVMTLHDGW
                     TVVTKDGSLSAHYEHTIAITHGQPLILTQEG"
     gene            11715..11987
                     /locus_tag="VFD89_09425"
     CDS             11715..11987
                     /locus_tag="VFD89_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HZJ58438.1"
                     /translation="MIEDNIMQIGRVVLAKAGRDRGNLFVIVDVIDHEYVFIANGTNR
                     TIDKPKKKKIKHLEPSPDVLSGIRDKILEGRKVFDAEIRKSLDVLL"
     gene            12118..12336
                     /gene="infA"
                     /locus_tag="VFD89_09430"
     CDS             12118..12336
                     /gene="infA"
                     /locus_tag="VFD89_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZJ58439.1"
                     /translation="MAKEDIIEVEGTVVEALPNAMFQVELENGHKILAHISGKLRMNF
                     IRILQGDKVTVELSPYDLTRGRITWRGK"
     gene            12352..12465
                     /gene="rpmJ"
                     /locus_tag="VFD89_09435"
     CDS             12352..12465
                     /gene="rpmJ"
                     /locus_tag="VFD89_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZJ58440.1"
                     /translation="MKVKPSVKPICEKCKIIRRKGKVMVICENPRHKQKQG"
BASE COUNT         3886 a         2401 c         3399 g         2783 t
ORIGIN      
        1 taatgcccgg ggacaacatc gacatggaag tggaacttat cacccccatc gccattgaag
       61 agggcctaag gttcgctatc cgtgaaggcg gcaggaccgt gggtgccggc gttgtagcca
      121 gcgttgcaaa gtaaatattg ataaaaaggg gcgacctgcg gtcgcccaaa cactgtttga
      181 acttagtttg aacttatcaa ataaagaact tgattttgtg ttgcatttgt tataaaattt
      241 aatagttgcg ctgcgtgtag ggacacatgt ggctgagtgt acacgggaga atctcctaaa
      301 gggatataag gagggaagac atggcaagtc agaaaataag gattaagtta aaagcctatg
      361 atcattcttt gattgatcaa tcggcggaaa agatcgttgg agccgcaaag aaaactggtg
      421 caaaggtatc gggaccaatt ccccttccta cggaaaggga tatcataact attttgaggt
      481 cccctcataa acataaagat tcccgtgagc agtttgagat gaggacccac aaacgcctaa
      541 tcgatatttt aagtcctact cctaaaacgg tggattcatt gatgagattg gatctgcccg
      601 ccggtgtgga cattgagatt aaattatagt tgattttcct gcattaactt agaatgtata
      661 aaaaaattga tctgtaggaa cggttaatgc agtgaaatta ctgaaaaggg taaagaggaa
      721 acatcatttc tcacagaacg gaggtagaaa gagatgaaga aagggattct ggggaaaaag
      781 ctaggtatga ctcagatttt caccgaagat ggcacggtca ttcctgttac cgttgtagag
      841 gccggtcctt gcgtggtcac ccagattaag acccaggata aggacggata tgatgctgtt
      901 caggtggggt atggcgatat tcgggaaaaa cttttaaaca ggccccaaaa aggccaattt
      961 gataaaaccg gtatctccta cagaaggcat atacgggaat tcccctttaa taatactgaa
     1021 gactataaga taggccagga aatcaaggcg gacgtcttta ctgtgggcga tcgtgtagat
     1081 gtagcaggca gatccaaggg taaaggattc gccggaaata tcaaaagatg gggccatgga
     1141 aaaggtccta tgagtcacgg ttcccattat cacaggggac caggggccat gagcgcagga
     1201 agttcgcctg caaaggtgct caagaacaaa aagcttccgg gcaggatggg taacgtacga
     1261 actacggtac agaaccttga agtggtcagg gtggataccg agaggaacct gttaatggta
     1321 aagggcgcta ttcctggagc caggggtaat atgcttatca tcaaagaaac ggtaaagaga
     1381 taagtcagca ggatgcagga aaggaggaaa ggatatgccc aaagtagcag tgtacaatat
     1441 cgaaggagca tcggtggatg aaattgaact cgacgataat atcttcggta ttaaaataaa
     1501 tgaggctctg ttgcatcaag tcgttagaat gcaacttaac aacaaacgtc aaggaaccca
     1561 atcaacccaa actcgtgctg aggtcagggg aggcggggca aagccttgga gacaaaaagg
     1621 tacagggcgt gcccgtcacg gcagcattcg ctctcctata tgggtaggcg gcggggtaac
     1681 cttcgcaccg aaacccaggg actacagctt taggatgcct aaaaagatgc gaagattggc
     1741 actaaagtcc gcactctcct ctaaagtgga gaacagccag atagtggtgc tggacgattt
     1801 aaaactggca cagccaaaga ccaaggaaat ggtaaaggtg ctgaaaaatc ttgaggtaaa
     1861 tggtaaggcc ctcttggtgt tacctgaaaa tgatgagacg gtagccaggg cttccggaaa
     1921 tattcagggg gttaagctgg cctctgtcaa taccttaaac gtccttgata tattgaatta
     1981 tgataaattc ataataacca aggaggctgc cttaaaggtc gaggaggtgt atgcatgatg
     2041 aaaaatccac acgatataat ccttagaccg gtattaacgg agaagagcta tgaccagatt
     2101 gcagacagga agtacacctt cctggtggat gtgagggcaa acaagacaga gataaaaaaa
     2161 gctgccgagg aaatattcgg agttaaggtt gaaggtgtca acacccttaa acagttggga
     2221 aaactcagga ggcaaggggt ccatatcgga cgcaggccta cgactaaaaa ggctattatc
     2281 aaattgacac ccgacagcaa gaccattgaa ttctttgagg gtatggcata aaacgacgac
     2341 aaggaggaag aaaaaatggc gataaagaaa tataatccca cctcccctgc caaaaggcat
     2401 atgaccgttt cctcatttga ggaggtaaca aagtttaccc ccgaaaagag tctccttgat
     2461 actctaaaga gcaaggcagg cagaaattcg gatggtcgta ttaccgttcg tcacaggggt
     2521 ggcggagcaa agagaaaata taggatcatc gattttaaac gagataagga cggagtaccc
     2581 gctaaggttg ctgggataga atacgatccc aacaggagtg cgaacatcgc ccttctccac
     2641 tatgtagacg gtgagaagag atatattata gcacccaacg gactcaagga cggggatatg
     2701 gttgtatcag gccccgatgc ggatatcaag gtcggaaatg cactgcccct tcagaatatt
     2761 cccctgggta ccgtaattca taacatagaa ttgagagtgg gaagcggcgg gcaattggtg
     2821 cgttctgcag gcaactctgc ccaacttatg gccaaagagg gcaagtatgg ccatgtcagg
     2881 ctgccctccg gtgaagtacg ccttgttctt ttaacctgta gggcgactat aggtcaggtg
     2941 ggcaacatag accatgagaa cattgcagtc ggcaaggcag gccgaaaacg ccatatgggc
     3001 ttcaggccat cagtgcgcgg ttctgccatg aatcccgtag atcaccccca tggtggtggt
     3061 gaaggaaggt cccccgtagg caggccatcg cctgttacgc cttggggtaa gcccacactg
     3121 gggttcaaga ccagacaaaa gagcaaaaaa tcggataagt atatcattag gaagaggaaa
     3181 ggctaatcct ccccgggatc tgtaatacct tatgctatta tagcctttag gaaatagggg
     3241 agtgaaagga ggcacaatat atgagccgtt caattaaaaa gggacccttt gtaagcgaga
     3301 gtcttatgaa gaagataata gggatgaata aatccggtaa gaaaaccgtc ataagaacat
     3361 ggtccagagc ttctaccata ttccccgaaa tggtaggaca taccatagct gtccatgacg
     3421 gcaggaaaca tgtacccata tatataacag aggaaatggt aggtcataaa ttgggggaat
     3481 ttgctcccac caggaccttt agggggcata gcgggcatac cgcaagatcc acttcgctta
     3541 agtaggtttt ttaaaggagg aaattaaagt ggcattgagg gtaaaagaaa aagcgttaaa
     3601 acgcagagaa aaccgtgacc agcgtcctaa agccactgcc aagcatatca ggatgtcttc
     3661 cagaaaagtc aaggccgtca tcgacctgat ccgagacaaa aatatcgatg aggcacaggc
     3721 tatattatta aatactccca gggcggcaac tcagccggta ctaaaggtac tgaactctgc
     3781 ggctgccaat gcggaaaaca atctggggat ggatcgtgat aatctatatg tggccgaggt
     3841 ctatgccaac caagggccta ccttaaagag atttagaccg agggcacatg gcagagctgc
     3901 cagaatcagg aaaaggacca gtcatattac catcattttg gacgaaaaga actaacaagg
     3961 agggatatgt tcatgggcca aaaaattaat ccacatggtt taagggttgg tgtcataaag
     4021 gattgggatg ccaaatggta tgcatccaag aaggactttg ccgacacctt actggaggat
     4081 catgggatca gggaattatt aaagaaaaaa ctatacgctg ccggcatctc aaagattgag
     4141 atagaacggg ctgccaatag ggtcaaggta aacatctata ccgctaaacc cgggatcgtt
     4201 atcggaagag ggggaagcgg ggttgacgcc ctaaaggtag agcttgaaaa gttcaccggc
     4261 aagacggtaa taattaatat agtagaggta aaaaatgcag atatggatgc ccagttggtt
     4321 gccgaaaaca tagcatccca gctggagagg agaatatcct acaggagggc tatgaagcag
     4381 tccatggcca gggcgatgag agccggtgcc aaaggcatca ggaccatggt ggcaggtaga
     4441 ctcaacggcg cagagatagc ccgttccgaa agatatcatg agggatccat tccccttcaa
     4501 accctaagag ccgatataga ctatggcttc accgaagcca ataccaccta cggaaaatta
     4561 ggggttaagg tatggatcta taagggcgag attctccccg gcgcaaagga aaatccgaga
     4621 acagaggggg gtaatcgtcg tgttaatgcc taagagagtc aagaggcgca gggtccacag
     4681 gggccggatg aagggcaaag ccaatcgcgg caataccatc acctatggcg aatttggtct
     4741 gcaggctata gaacccggtt ggattaccag caaccaaatc gaagcagctc gtgttgccat
     4801 gacccgttat atcaggaggg gtggaaaggt ttggattaaa atcttccccc ataagccagt
     4861 aacggcaaag cccgctgaaa ccaggatggg tagcggtaaa ggcgcccccg aatattgggt
     4921 agccgtggta aagcccggca gggttatgtt tgagattgcc ggtgtttcgg aagaaatagc
     4981 cagagaagcc ttgagattgg catctcataa gctgcccttg aaagctaaat tcatacaaag
     5041 agaagaaatg ggtggtgaaa gcagtgaaag ccaatagatt tagggaactg agcgatgagg
     5101 aattgcagga cagagtagct gaatttaaga gcgagctttt taatcttcgc tttcagctag
     5161 ccaccggtca attggaaaac cctatgagga taaaggaagt cagaaaagat atagccaggg
     5221 tgaagactat aattcgtcaa agagagctaa attctgcaag gatctaagtt gaagggagga
     5281 acattccatg gcagaggacg caagagccat aagaaaagcc cggaccggag tggttgtcag
     5341 caataagatg gataagacta tcgtagttgc cgtagagaat cgtaccaagc acaagctgta
     5401 cggtaagatc ataaagagaa ccatgaaatt aaaggctcat gatgaaggta acgaatgcca
     5461 aatcggcgat cgagtaaggg ttatggagac caggccctta agcaaggata agcattggcg
     5521 tttagttgaa ataattgaga aagctcagta atttacacgc tttataactg agaggagggt
     5581 acaagatgat acagcaggag agccggctga aggtcgctga taatacagga gcgaaggaag
     5641 tattgtgtat tcgtgttctg ggcggaaccc gcagaagata cgcaaatata ggcgatgtag
     5701 tagtagctac tgttaaaagc gcaacgcccg ggggcgttgt taaaaaaggt gagattgtta
     5761 gggctgttat cgtaagatcc aaaaagggaa ttcaaagaaa cgatggaacc tctattagat
     5821 ttgatgataa tgcagctgtc attataaacg atgccaggca gcccagaggt acccgtatat
     5881 ttgggcctgt cgctagagaa ctgagagaga aggactatat gaggatcata tctctcgctc
     5941 cggaagtact ttaacttgag ggaggtggac aatgatggct gtaccaaaaa ggaaactcca
     6001 cgttaaaggt ggagacacgg tggagattat ttcgggtaag gataaaggca aacgtgggaa
     6061 ggtactaaag accctcccta aagaaggcaa gataatagta gaaaagatca atatgctgac
     6121 ggtgcataca aagccacggg gaatggagca gccgggaggg atcattcata gggaaggtcc
     6181 tatctatgct tccaaagcca tgccggtatg cgaaagatgc aataaaaaga ccaggattgg
     6241 tagacgagta ttagaggacg ggtctaaggt gcgcgtatgt agggtatgcg gcgaaacctt
     6301 taatgactga tgcaccgaag ggaggtaagc agagagtggc tcgattaaaa gaattatatg
     6361 aaaaagaaat agttcccgca ctgatggaaa gatttaatta taagaacgtc atggaagccc
     6421 caaagcttga caagattgta attaatatgg gggtcggcga cgccaaggag aacccgaagt
     6481 ttctcgaaag tgccgtaaat gacctcagca tcatagccgg ccaaaagccc atcgttacgg
     6541 cggccaaaaa gtctgtatct aactttaagc ttcgagaggg aatgcggatc ggcaccaagg
     6601 ttacgcttcg cggggacagg atgtacgaat ttgctgatag attattaaac gtcgctcttc
     6661 cccgggtaag ggactttaga ggagtttcgg ccagatcctt tgacggtcgg ggcaactatt
     6721 cgctggggat aagagaacag ctgatctttc ccgagatcaa ctatgatcag atcgaaaagg
     6781 tcagaggaat ggatatagta tttgtaacca cagctaaaac tgatgaagaa tcacgtgaat
     6841 tcttaagatt attgggaatg cctttcgcaa attagtgata aggagggaaa gcgagtggcg
     6901 aaaaaatcat ggagagttaa gcaacagcgt gaaccgaagt tctctactag ggcctataac
     6961 agatgccgca tctgcggacg atcccatgcg taccttagaa aatttggttt atgccgtatc
     7021 tgcttcagag aattggctta taagggtcaa atacctggtg tcagaaaagc cagctggtag
     7081 actggagaga ttggaaggag gttaaatata tggttatgac cgatcccatt gctgatatgc
     7141 taacccgtat tcgcaatgca ttagtcgtca agcatgcaag tataaatgtt cccgcctcta
     7201 atatgaagat ggctatagcg aatatcttgc gtgaagaggg ttttataaga gggtttaccc
     7261 gaatggaaga cggtaaacaa ggtgttatta ggatagattt gaaatacggc caaaatggtc
     7321 aaaaggtaat cgcaggctta aagagaataa gtaagcccgg ccttagggta tatgccaaca
     7381 aagatcaggt gccgaaggtg ctggggggac tgggggttgc catcctttca acatcaaaag
     7441 gtgttatcac agataaaaaa gcccgtgaag aaggggttgg cggagaagtg ctctgcttta
     7501 tttggtaaat tagaagttgg aagttagaag ttagaaacca gacctttaac atctaacacc
     7561 gaaaggagtt gtaagaggat gtcacgaatc ggaaatcttc ccatagacat accccagggc
     7621 gtaaccgtta ccgtggatgg gaacaatctg gttaccgtaa agggcgccaa aggggagctg
     7681 tcccagccta ttaataggga aatagcagtc acggtggaag ataatgttgt aatcttgaag
     7741 agatcttcgg acgcgaaatt tcaaagatcc attcacggcc tttcaaggtc ccttgtggcc
     7801 aacatggtgg aaggcgtgtc caagggcttt caaaagaacc tggaaatcaa cggtgtggga
     7861 tacagggccc aaaaacaggg caagaagctg attttggccg tagggtattc ccatccggta
     7921 gagatagaag aagctgatgg ggtggaattt gaagtgccgg ccgctaaccg aattattgtc
     7981 aggggaattg acaagcaaaa ggtgggggct atggcagcga aggtaaggga tacaagaccg
     8041 ccggagccct ataagggtaa aggcatcaaa tacgagaacg agagaattat tcgaaaagaa
     8101 ggaaagacgg gagcataagc cgggaaggag tgacacaggg tgattaaaaa aattgacaaa
     8161 aatgcagcac ggaaagtacg ccacctaagg gtgagaaaca gagtttccgg ttcggtggag
     8221 ataccgcgcc tcaacgtttt ccgaagcctc aaacacatct atgtccagct tatcgacgat
     8281 aacggagggc ataccctagt atcagcttcc agcttagatc cggagattaa gagcgtttct
     8341 gataagacta ggaaagaagt tgccaagatg gttggggaac tggctgcacg cagagccctg
     8401 gaaaaaggga tcaagaaggt agtttttgac cgtggcggat atatctatca cggcagagta
     8461 tcagaagtag cagccggtgc acgggaagcc ggacttgagt tttaaggagg gaaatccatg
     8521 gagcagatag atgccagcac gctggatctt aaggagaagg ttgttaccat caaccgagta
     8581 gccaaggtag taagaggtgg cagaaacttt cgctttaaca cagttgtggt ggttggtgat
     8641 gagaacggac atgtaggcgt tggaacggga aaggctaccg agatccccga agctattaga
     8701 aaagggattg aggacgcaaa aaaacatata atgaaggtgc ccattgtcaa cacgaccata
     8761 ccccatgagg tgaatggacg tttcggcagc ggtagcgtat tgatgatgcc ggcaaaggaa
     8821 ggtaccggag ttatcgcagg cggacctgta cgtgcagtac tggagcttgc gggtattcgg
     8881 gacatattaa ccaaatcttt gggatcaagc aacccaagaa atatggttaa cgccgctatg
     8941 gaaggattgt caaggctcaa aacagttgaa gacgtggcaa gacttcgcgg caaggctgtc
     9001 gaagagatct taggttaggg aggcgaagcc tgtggctaaa ctaaaggtga cccaggtccg
     9061 gagcaccatc ggatccaaga aggatcagat agcaactatg gaggccctgg gacttagaaa
     9121 gattagaagt gaaaagatac acgatgattc cccgactatc cgcggaatga tcgataaggt
     9181 caagcatcta gtatccgtcg aggagattga cgcttaaggg aggtgaatgc attgagactt
     9241 aaccgattga gtcctgctga aggttcaact aaaacctcta agagaaaagg gagaggtccg
     9301 ggttcaggcc ttggaaagac ttccggccgc ggccataagg gtcaaaaagc cagaagcggc
     9361 ggcggtgttc gtcccggatt tgagggtggg cagatgcccc tcaccaggcg gctccccaaa
     9421 agaggcttta ctaatatatt cgctaaggtt tataatgaag tgaacttggt gcaattggat
     9481 gtttttgagc aagatacaat agttacaccg gagctgctta aaaaagaggg aataataagt
     9541 aaggaaaaag acggggtcaa gatattgggc aacggcgagc tgaatattaa gctcattgtt
     9601 aaggcccata aattcagtaa agcggctaaa gaaaaaatcg aggctgctgg gggaaaggcc
     9661 gaggtgattt aaagtgttgg acaccttgcg taatgcctgg agaattgaag atatcagaaa
     9721 aaagattgtc tttaccatga ttatgatatt ggtgtatagg gtggggtcct ttattcccgt
     9781 gcccttcatc gatgccagtc atataaagga tctggtcggg gaaggtaatc tgttgggatt
     9841 gcttgatatt atatcagggg gcgcctttgc gaactatacc atatttgcta tgggtataac
     9901 cccatatatc aactcctcaa tcataatgca gcttctgacg gtagctattc caaagttgga
     9961 acagctgtcc aaggagggtg aagagggcca gaaaaagata acccaatata cccgctacct
    10021 gaccataggt ttagcgtttg tacaatcagc gggaattacc tacggacttg gaaaccaggc
    10081 cgggggacag ataatgacga atcattcggt atgggccttc tttgtagtcg caatgactct
    10141 gaccgccggt acagcgttct tgatgtggtt gggggaacag attacagaaa acggcattgg
    10201 caacggaata tccatgatca tctttgcaag catagtttcc aggatacccg tagctgcggt
    10261 gagcctgtgg aatctggcct ttgaagtggg gaccataagc ccatggtcat taccattgat
    10321 cgctatattc atagtggctc tcattgcagg cgtggtcttt atcgatatgg gtcagagaag
    10381 gatacctgta cagtatgcga aaagggtggt aggtaggaag gtttatgggg ggcagagcac
    10441 ccacataccc atgaaggtta actcctccgg ggttcttcct ataatcttcg ctgtttcatt
    10501 gctgtccttg cctcagacaa ttggatactt ttttccagag ggcgggttct tccgctttat
    10561 tgaggctaac ttcagccaga aatctgcggc ctatgccata gtgtacgcac tattaatcgt
    10621 attttttacc ttcttttata gccagatctc ctttaatccc attgaggttg ccaacaactt
    10681 aagacaatac gggggatttg tacaagggat aaggccggga aaacctacat ccgattatct
    10741 tttaagaatt tccaaccgca ttaccttggt aggttccttg ttcttggctg cggttgcggt
    10801 aatacccata gcggtagcag gatttactaa gattcccatg taccttcagg ggaccagtat
    10861 actcatcctg gtaagcgtgg cccttgaaac ctctaatcag ttggaagccc aaatggttat
    10921 gaggcattat aaaggctttt taaagtagga gattatcgtt gattatatta aaatcaaaga
    10981 gtgaaataaa attgatggaa gaagccggaa ggatcgtggc cggggcccat gaggtcgtta
    11041 aaaatgccat agccccgggt atcactacgg cagagcttga cgagatagcc gaggattata
    11101 tccgaagtcg gggtggtatt cccgcattcc taggatacca gggttatccg gccagcatct
    11161 gtgcatccat aaatgagcaa gtagtccatg ggattccggg tcccgttgcc cttaaagaag
    11221 gcgatatatt aagcgttgat atcggtgcct ttcataacgg gtactgcggt gatgcggcga
    11281 gaactcatcc cgtcggggaa gtaacacccg atgcccatag actcattgaa acagcaaaag
    11341 aggccttttt cagcggactg gaattcgccc gggaaggata ccgactttcc gatatatccc
    11401 atgcaatcca agtatatgta gaagggaaag ggtactccgt ggtaaaggcg ttggtcggtc
    11461 atggcatcgg ccaggctatg cacgaggaac cccagatacc aaacttcgga cctccgggaa
    11521 gggggcccag actcaggaaa ggcatggcat tggccatcga gcctatgata aatgcgggag
    11581 cccatgaggt aatgacactc catgacggct ggacggtagt aacaaaagac ggcagccttt
    11641 ctgcccacta tgaacatacc attgctataa cccatgggca gcctttaata ctgactcaag
    11701 aggggtagat aaaggtgatc gaggataata taatgcaaat aggaagagta gtgttggcta
    11761 aagccggaag ggacagaggg aacctctttg taatagtcga tgtgatagat catgagtatg
    11821 tatttattgc caatggcacc aataggacca tagacaaacc gaagaagaag aagatcaagc
    11881 acttagagcc cagtcctgat gtattatcag gcataaggga taaaatcctc gagggccgga
    11941 aggtgtttga tgcagagata agaaagagtc tcgatgttct gttataggga ctaattacga
    12001 tatctgatgg gaagtatggg tgaggaaact tcaaaaacgc atttattaaa tcttttgaaa
    12061 ctttatgtga cgagtaattt ttgttgacac ctaaaaggaa aagggagggt ggattgtttg
    12121 gccaaagagg atattattga ggtagaggga actgttgtag aagcgttgcc caatgccatg
    12181 ttccaagtgg agctggaaaa cggccataag atattagccc atatatccgg taaacttcgt
    12241 atgaacttta tcaggatatt acaaggggat aaggtgacgg tagaactatc gccctatgat
    12301 ttgacccgtg gtcggattac ttggcgaggt aagtgataag gaggtaatgg gatgaaagta
    12361 aaaccgtctg taaaacctat ttgtgaaaaa tgcaagataa tcaggcgtaa aggtaaagtc
    12421 atggttatct gcgagaaccc cagacataaa caaaaacagg gataagatg
//
LOCUS       DASRNX010000236         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_528946,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000236 DASRNX010000000
VERSION     DASRNX010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528946"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..872)
                     /locus_tag="VFD89_09440"
     CDS             complement(33..872)
                     /locus_tag="VFD89_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZJ58441.1"
                     /translation="MGSYAKRKTLNLVFVYILAILLSIIFVIPLIFTFSNSLRDLYSS
                     PVLIPKTLNWSNYNLAVTLIPFWKYVGNSGIITGISVSLAICMNFLFGYAFARLKAPG
                     KNAMFILVLSMLMVPGIAVQIPQFIIFSNIGIKDTYWIWILGGLGGNAFYIFLFRQFL
                     SSLPKDLEDAARIDGCTTFTIIWRIFVPLSWPAIAVVAMLDFNGAWGDYMTGYMFLSP
                     KNYPLSTALISTSYSLPNQPTIQLEPVVNAAALLLTLPVIVVFFLGQRYLVEGIVTTG
                     IKG"
     gene            complement(874..1836)
                     /locus_tag="VFD89_09445"
     CDS             complement(874..1836)
                     /locus_tag="VFD89_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZJ58442.1"
                     /translation="MISTSDRKKTAKIKSPMSLKKRETIQFYTIIAPFVLMFIGIRLI
                     PFLWGIFLSFTNFTGFNYANLRFVWFDNYLRVFKDNDALYSLWRTLQISLVIVPMGLV
                     IGFLLSVLLNNPIRGIGVFRTIYYLPSIVPAVAIGLMWKAIYNQNGGLFNGILRALGM
                     NGIPWLGYDWVTRSLIIMMAWGAGGGILIYLAALKGIPKELYESASIDGAKPIRQFFY
                     ITIPMMTPIIFYNLIMGIIGALQLFAQPVLLTTGSSGILNTPLRPNYTYQVHVYQQIF
                     ANQRFGYGLSMIWVIFIIILLLTFTVFRSSQFWVYYEVQQDGGK"
     gene            complement(1941..>3388)
                     /locus_tag="VFD89_09450"
     CDS             complement(1941..>3388)
                     /locus_tag="VFD89_09450"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HZJ58443.1"
                     /translation="IIRCDIILVTRYITRHQGFCSQFKNEEEFVVQKKTLVLSICVMV
                     VAVLVLAGCAGGGPASKGGGSKGEVTITTNFTEGEVPGDILPNFENDNPNIKVETVEA
                     TDEKLMAMIAAGNAPDVIRIYGARDIPTFVSRNLALNLDSYFDNSPIFKNKDDFLPVV
                     DVYRFDGEKQGAGPYYGFVKDYSLDNTLWANKKVFEDANVPIPDSSKPLTYSELFEIA
                     EKLTVKEGDETTRFGYGQTFGSGFDYDTLMLAMLQKGADVWSDDFNSAKFNSPEVKGF
                     MKQWYDFVKTGACISPINVSTVGPLDLFMEDKVGIITSGYWYSGMIRSNENVADRMED
                     FIHLPSPIIDGGERISTTTAACGGIIFKNSEHPDEAFKFFEYFLGGEPANDRARSGWG
                     IPAFKSKLELLPQETEFDKQSFNVLMEELKYSEHMLPYNPYTNYTSIQSAVDKIFSPV
                     YFDKDTLDDAADKLNSEAELLIQEGKDIMGK"
BASE COUNT          981 a          755 c          695 g          957 t
ORIGIN      
        1 tgaaataatt aaattgtctt tatctgtaat agttaaccct taattcccgt agttacaata
       61 ccctcaacca gatatctttg acctaagaag aacactacaa taacgggcag tgtcaacaga
      121 agcgccgcgg cgttaactac aggttccaat tggatcgtgg gctgattggg taaggaataa
      181 ctagtgctta ttagggcagt ggataatgga tagtttttag ggcttaagaa catatatccc
      241 gtcatataat cgccccatgc cccgttaaaa tccagcattg cgactactgc tatcgccggc
      301 caggaaaggg ggacaaatat ccgccaaata atagtaaatg tggtgcaccc gtcaattctc
      361 gctgcatctt ctaagtcctt tggcaacgac gagaggaatt gtctgaatag gaatatatag
      421 aaggcattgc cccctaggcc gcctaagatc caaatccaat aggtatcctt tatcccaatg
      481 ttgctgaaaa ttataaactg tggaatttga actgctattc cgggtaccat aagcatcgac
      541 aggaccagaa taaacatggc atttttcccc ggagccttaa gcctggcaaa cgcatatccg
      601 aaaagaaaat tcatacatat tgctaggctt acggatatgc ccgttatgat ccccgaattt
      661 ccaacgtatt tccaaaaagg aattagggta actgccaagt tataattgct ccaattcaac
      721 gtttttggaa tcagaacagg tgaagaatac aaatccctca aggagttact gaaggtaaat
      781 ataagcggaa taacaaaaat aatgcttaaa agtatagcaa ggatatagac aaataccaga
      841 tttaacgtct tacgctttgc ataacttccc atatcattta cccccatcct gttgaacttc
      901 gtagtacacc cagaattggc tcgatctaaa cactgtaaat gtcaagagca gaattataat
      961 gaatataacc cagatcatgg acaggccata gccaaatctt tgattcgcaa aaatttgctg
     1021 atagacgtgt acttgatagg tataattggg cctcagtggt gtatttaaga tcccgctaga
     1081 tccggttgtt aataataccg gctgggcgaa gagctgcaga gcaccgatta ttcccattat
     1141 aaggttgtaa aaaattatcg gggtcatcat gggaatagtt atataaaaga attgtctgat
     1201 gggtttagcg ccatctatag atgccgattc atataattct tttggtatcc ccttaagagc
     1261 tgcgagataa atgagtatcc ctccaccggc accccaagcc atcataatga tcaaagagcg
     1321 cgttacccag tcatatccga gccatgggat tccgttcata cccaaagcgc gcaatatgcc
     1381 gttgaaaagt ccgccatttt ggttgtatat ggccttccac attagaccta tggcgactgc
     1441 cggtactatt gaaggcagat agtaaatagt cctgaatacc ccgatacccc tgatggggtt
     1501 attaagcaaa acggaaagca gaaatcctat aaccaagccc atagggacga tgaccaaaga
     1561 aatctgcagg gtacgccaca gggaatacag agcgtcattg tctttaaaga cgcgaagata
     1621 gttatcaaac catacaaagc gcaggttggc ataattaaat ccggtaaagt tagtgaaact
     1681 taaaaatata ccccataaaa agggtatcaa tctaatccca ataaacatca aaacgaaagg
     1741 ggctatgatc gtgtaaaact gtatagtctc cctttttttc aggctcatag gtgattttat
     1801 ctttgctgtt tttttacggt cactcgtact aatcattgac aatcaaattc cctcccccaa
     1861 atttttatta tggataagta gtccaaaaac ggactactta tccatgtgca gatttgaatt
     1921 tcttttaatt taatacagtt ctatttcccc attatgtcct taccctcttg gatcaacagt
     1981 tcagcttcgc tattgagttt atccgctgca tcgtctaaag tatccttgtc aaagtacaca
     2041 gggctgaata tcttatcgac agcactctgt atagaggtat agttggtgta cgggttatag
     2101 gggagcatat gctcggagta ctttaattcc tccataagta cattaaagct ttgcttatca
     2161 aattcggtct cttgcggaag caattccagt ttggacttaa atgcgggaat accccatccg
     2221 gagcgtgcac gatcgtttgc cggctctccg ccaaggaagt attcaaagaa tttgaaagcc
     2281 tcgtcgggat gttcggagtt tttgaatatt atgccgccgc atgctgcagt agtggtgctt
     2341 atccgctcgc caccgtcgat tatcggtgat gggaggtgaa taaagtcttc cattctgtcc
     2401 gcgacgtttt cattgctcct gatcattccg gagtaccaat aaccggatgt aataattcca
     2461 actttatctt ccatgaaaag atccaacggt cctacggtgc taacatttat agggctgata
     2521 catgccccgg tcttcacgaa atcgtaccat tgtttcataa agcctttgac ttccggtgaa
     2581 ttaaactttg cgctgttaaa gtcatcggac catacatccg cacccttttg cagcatggca
     2641 agcatcagtg tatcgtagtc gaatccgctg ccaaaggtct ggccataccc aaatcgggta
     2701 gtctcgtcgc cttctttaac ggtcaatttt tcggctatct caaatagttc gctgtaggtt
     2761 aggggtttgc ttgaatcggg aattggaacg ttcgcatctt cgaagacttt tttattggcc
     2821 cacagggtat tgtccagact atagtccttt acgaatccat aatatggtcc ggcaccttgt
     2881 ttctcgccgt cgaagcgata aacgtcgaca acaggtaaaa agtcgtcctt attcttaaag
     2941 atgggactgt tgtcaaagta actgtccaga tttaatgcga ggtttcttga tacaaaggtt
     3001 ggaatatctc ttgcaccgta gatacgtatt acgtcaggtg cgttaccggc cgcaatcatg
     3061 gccataagct tttcgtcggt cgcctctacc gtctctacct taatgttggg attgtcgttt
     3121 tcgaagttcg ggagtatgtc accgggaacc tccccttccg tgaagttagt tgtaatggtg
     3181 acttcgcctt tagagccgcc gccttttgaa gcgggaccgc cgccagcaca accggccaat
     3241 acaagaaccg ctactaccat cacacagata ctaagtacca aggtcttttt ttgcacaaca
     3301 aattcctcct cgtttttaaa ttgagagcag aacccctgat gtcgtgtgat atatctagtc
     3361 accaatataa tatcacaccg tataataa
//
LOCUS       DASRNX010000237        12381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_366313,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000237 DASRNX010000000
VERSION     DASRNX010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 12381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12381
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366313"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..524
                     /locus_tag="VFD89_09455"
     CDS             72..524
                     /locus_tag="VFD89_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58444.1"
                     /translation="MYRQEDFDSVKKQVMRTLVLLLVGLIFFIVISVIIAKRIENWAG
                     MVFLLLGVSAGVFIWGVYVAPVFAYFRFLKDLITGKTRQIHGRAVEVSSHPVYKDNKL
                     YFYEVGIEEDGVERLLLLDDQKEWPKINISESYNFEVHENFITDIEPA"
     gene            633..1595
                     /locus_tag="VFD89_09460"
     CDS             633..1595
                     /locus_tag="VFD89_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HZJ58445.1"
                     /translation="MKYRKLGRYGVRVSEISLGSWLTYGASYGKERTEACVRKAYDLG
                     INYFDTADVYGMGQTHAGAAEEVLGEILPDFPRSSYVLATKAFWPVGDGPNDKGLSRK
                     HIMEQCNASLKRLNTDYVDIFYCHRYDPDTPVDETLRAIEDLIRQGKVLYSGVSEWSA
                     TQIADGVHTARHLNLNPIAVNQPIYNMLNRYIEDEVMPLCSREGIGLAVFSPLAQGIL
                     TGKYKRGQEVPKDSRAADESAGRGISRQLNDETLTKVEKLEAIASDLGMPLTQLSLAW
                     ILTHPEISSVIIGASRPEQIEENVKAAGKTLDEDTMDAIEEILD"
     gene            complement(1651..2076)
                     /locus_tag="VFD89_09465"
     CDS             complement(1651..2076)
                     /locus_tag="VFD89_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58446.1"
                     /translation="MKRFCHTLIPIFILILLVSGCNKSNNPNPVWGSTEAPDAEIQHK
                     SISILNMSDLEIIKKSSDFLKSCGKTLIFEETRILYENNLDTDVLCKNKDNKDVSYKG
                     EYLAIYFYPHAKMQEQENILIVYIGDGGQVLGYREVAGA"
     gene            2395..4203
                     /gene="pepF"
                     /locus_tag="VFD89_09470"
     CDS             2395..4203
                     /gene="pepF"
                     /locus_tag="VFD89_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012686206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HZJ58447.1"
                     /translation="MKDLSKGKLPQRGEIPKEFRWKLGDIYKSASHWEDDYRWVKENL
                     PSLKEHEGNLGESAQNLLSGLDTYTGLMKRVEKLYVYAHMKKDQDNANTQSQGLLDRA
                     QGLMVEFGGFTSFLVPEILEIPESRIKEYIEALDGLMPYGHYLGDIMRRKEHVLSPKE
                     EKILAMAGEVAAAPEHIFGMVNNADMRFPYITDENGHEVELTHGRYLQFMESRDREVR
                     KQAFDELYKTYGKQKNTIAATLNYSIKKDIFYSKIRNYPSTLENALHSDKVPVLVYDN
                     LIKAVEDNMDAMHRYVSLRKRALGLDELHMYDLYVPIVRDVEMKIPYNKAKDMIGEAL
                     NPLGDNYLDILEEGFNSGWIDVLENEGKTSGAYSWGCYDTHPYVLMNYQENIDNVFTL
                     AHEMGHAIHSYLSNKNQPYILAGYKIFVAEVASTLNEILLTNHLLENLEDDRQKAYVL
                     NYYLEQFRGTVYRQTMFAEFERIVHGMAEGGEPLTVESLSEAYYKLNLKYYGPDMVVD
                     DEIALEWARIPHFYSSFYVYKYATGFSAAAALAQRILDEGQPAVDRYLNFLSRGGSDY
                     PIELLKGAGVDMTSPKPVNQALKTFSALVDRMGKLL"
     gene            4249..5130
                     /locus_tag="VFD89_09475"
     CDS             4249..5130
                     /locus_tag="VFD89_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58448.1"
                     /translation="MIRNILKFLKIIILATLIVLLCVDIVGVYLGLILGKTVLGPRLL
                     IETLKRHEAYAQIRQLIFKLVKGSLPNGRDSIPYLEKAISESWLEQEINMLLKNYYAF
                     ARGERKDTPIIFFSGLKRRVVDALDGDQPHGNRIRLVKFWFDPLPDEVRLEDFMSVDL
                     LWGIRGAFDLMLRIPWIMGGIALFIILIMHLLLLDWRQSMLWFGAAIITAGALLILMG
                     IAAVWAAGHMSILANMKDRLLSLDIPKANVVNFFNSFLNGIINPMNIIGTLSILTGGM
                     LIYFVPIAEEPPKPVTE"
     gene            5317..>12381
                     /locus_tag="VFD89_09480"
     CDS             5317..>12381
                     /locus_tag="VFD89_09480"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZJ58449.1"
                     /translation="MEKPDVLYSEARDLAEEHKGIVYRKSVNRDFLKIEQDSQILLEA
                     YRDINEQVKAKQDIIPAAEWLLDNFYMVEEQSKQIQHELPNNFRELPILKSGIPRVYE
                     IAEGIVSFRSGQLDEEPIIGFLKEYQSVTPLNNGELWILPLMIKVSLIKRIRERAVHI
                     VEYQKQRNRGRRWGALLVETIDAPGDELQRLIMEHDREVGYMLPSYAEGMLRVFRDSG
                     SKGASLMMWIDGKLALQGTNSDELIQGERQHQSDCQVSIGNYIISLKFLLSIRWEEIF
                     EELSNLDGILNKDPSGFYSDMEFTSRNYYRTELVKIARRYRVSELEVARLAVDIAEDT
                     MEPTGGDIRLGHVGYYLVDQGRRELKERLGGKHPKEGWLKEHPTAFYLGSIGIIVAVT
                     IFLMLNYLSGLSPAQNPLILVLSGIVIFIPVVTLAVGLTHWIIPRIFPPCYLPKLELE
                     DGIPEEYRTMVIVPTLLTSEKRVWELIEQMEVFYLANQGDNIHFALVGDFKDSKNEKE
                     PDDEAIIDAATKAVEELNQRYSRGDGGIFYFFHRHRQWTPAQKSWMGWDRKRGALVEF
                     TGLLRGREDTSFSTQVGDMSMLPKIKYVITLDADTNLPRDVAKRLVGTLAHPLNRPIV
                     DKSLNRVVEGYGVLQPRIGVSVDSASRSSFALTFSGQTGVDPYTTAVSDVYQDLFCEG
                     IFTGKGAYDVDVFNDILSSQIPENSILSHDLLEGSYIRAGLVTDIELIDGYPANYISY
                     AMRSHRWVRGDWQLLPWLFGKVKNAKGERIRNPLSAISKWKIFDNLRRSLLSPSLFLL
                     LTLGLTVLPRSDWFWLGLFTITLTLPLLMDVVASFIGRVGSPNRFAGDILYGTKNLAM
                     QIILTFVFLAHQAYLMTDAAIRSVVRVFFTRKNLLEWVTAADTDRRFKGTLEDYWKKM
                     LPAMLISVAFFIWVVTFRRSFLFIPLAISLIWLISPLIAYRISQPKYRRESDLSKEQV
                     HRLRMIGRRTWKYFDEFVGAGDNWLPPDNYQEEPHVGIAHRTSPTNIGLALISTLTAR
                     DLGYISTTQAIERFENTFRTLRRLEKWNGHYYNWYNTRTLEPLKPRYVSSVDSGNLAC
                     YLILLKQGIAELLSRPLVGKEVALGIRDTMDLDFEKSGRSIPPIINMLEASENITLTE
                     WKLTLNQLGNIGNYTDRQIHQYREEMAAMVPWVDLLLDVPRSILGENGLPAEAKESFG
                     ILMDKLNKEFSLKKILDDYHYVLDKLSRIILSLRRTKNPDKESNGVATWLKQLEIALG
                     ESHMAARKMAHRCKKLMDIIDEIFQDMDFATLYDKKKELFSIGYDIEAGELQDVYYDL
                     LASEARQTSFVAIAKGDVPQKHWFKLARSLTMVGDSRSLLSWGGTMFEYLMPMLIMKN
                     YRNTLWDETYQAVLKGQRQYSDQRHLPWGVSESAFYAFDLQLNYQYKAFGVPKLGLKK
                     GLIKDMVVAPYASIMGLNIDPLAVMKNIEFLESEGASGKYGMYESIDYTPERVPSRKK
                     SMLVKTYMAHHQGMILLAFNNYLNNCVMQERFHAIPMIRATELLLQERIPQREIVIKE
                     HSDAFDIPDMDTEMKSYQEIHARNIIDTAHTPAPEVCVLSNNYYTTIVTNSGGGFSQY
                     QELAINRWREDVTRDNWGMFFYIKNLNSGNYWSSGYQPCEDSGEKYEVVFEGDKAIFS
                     RKDGNIETKTEITVSPEHSVEIRRITLNNHSEHERAMEVTSYFEPILTLQGDDTAHPA
                     FVNLFVETEYVPEHDILVMTRRPRSKDYKQMWLAHTMIAEGEPIGVVQYESDRSKFIG
                     RNRGLKHPVAMDPDQPLSNTVGAVLDPVMCLRQRIGIGPGQAAKLSYMVAVADTRDEA
                     INLAREYRNTAVISRAFELAWTHSQVEMRYLNISSTEVNLYQKMLSSIIYTNPARRAI
                     LKDIMNEEDQSRLWTYGVSGDLPIATIRITNTEQLEMVRQMLSIHEYWRLKGILVDLV
                     ILNEYGNSYEQPVQDRLREMLMVSHLRELQDKPGGVFLLAGNLMPDGDKNLLAYASRI
                     TIDGEMGSVASQLEANGSGDKLPDAMEYVGSEKTEEDEIKEYGELLYFNNIGGFTPDG
                     REYEIRLQGGNATPLPWSNVVANEVLGFLVTESGAGYTWYGNSRENKLTPWSNDPVMD
                     PAGEAVYVRDEDSGAVWSIAPTPIRTDGDYTIRHGQGYSVFEYDRNRLRQKQTMFVVP
                     DQPIKYYHIGLTNDSQKVRKLTITFYVEWVCGVNPIPNNQFIVTGEDRAINAILAYNP
                     YNGEFGNRVAFIACDKPYTSITGDRTEFIGRNGSLRDPAALGRVGLSNRVGPGLDPCG
                     VIQVALEIGPGEGEELVFVLGQGDDEAQVRQLIGDYTNMAAAQSALEGA"
BASE COUNT         3469 a         2703 c         3291 g         2918 t
ORIGIN      
        1 gatgcacatc gctaaataca tatgctataa ttatctcaat gaaattattt ggacaatcgg
       61 gggtatgaat tttgtacagg caagaggatt ttgatagtgt aaaaaaacag gttatgagga
      121 cattagttct gctgcttgtc ggcctgatat tcttcatcgt aatctctgta atcatcgcaa
      181 aacggataga aaattgggcg gggatggttt ttttgctgct gggagtcagc gcaggagtat
      241 tcatttgggg agtatacgta gcccctgtct tcgcgtattt tcggttctta aaggatctga
      301 taaccgggaa aacaagacag attcatggcc gggcagtgga ggtcagttcc catcccgtat
      361 acaaggataa taagctatac ttttatgagg tcggtattga agaggacgga gtggagaggc
      421 tgctcctact ggatgatcag aaggaatggc caaagatcaa tatcagcgaa tcctataact
      481 ttgaggtgca tgaaaacttt ataactgata tagaaccggc ataggaaaca tgaaacatat
      541 catggaagta ccgctaccgt tcaccatttt gggtttgatt tttatgggat aggttaatat
      601 atattatgaa ttaaattcta ggagtgatga ttttgaagta tagaaagcta ggcaggtacg
      661 gtgttagggt aagtgaaatt tccttgggca gctggctgac ctatggggcc tcatatggaa
      721 aagaacggac cgaagcctgt gtcagaaagg cctatgacct gggaatcaat tattttgata
      781 cggcggatgt ctatggcatg ggacagaccc atgcaggggc tgccgaggag gtcctaggcg
      841 aaattctgcc ggattttccc aggagctcct atgtattggc caccaaggca ttttggcccg
      901 taggggacgg tcccaatgat aagggcttgt cccgcaagca tatcatggag cagtgtaacg
      961 ccagtttaaa gcggcttaat accgactatg ttgacatctt ctactgtcat aggtatgatc
     1021 cggatactcc ggtggatgaa accttgaggg ctatagaaga tctaatcaga cagggcaagg
     1081 tcctatattc cggggtcagt gagtggagtg ccacccagat tgcagacggt gtacatacgg
     1141 caagacatct aaatctaaat cccatagcgg taaatcagcc catatacaac atgctcaaca
     1201 ggtatataga ggacgaagtt atgcccctat gcagtaggga aggcataggg ctcgcggtgt
     1261 ttagtccctt ggctcaaggt attcttacag gcaaatataa gagggggcag gaagtcccca
     1321 aagacagcag agccgccgac gagagtgcag gccggggtat atccagacag ctgaacgatg
     1381 agacattgac taaggtagaa aaattggagg ctatagcctc ggatcttgga atgcctttaa
     1441 ctcagctgtc ccttgcctgg atacttacgc atccagagat aagcagcgta ataatcggag
     1501 catcaaggcc tgagcaaata gaggagaatg taaaggctgc aggcaagacc ttggatgagg
     1561 ataccatgga tgccatagag gagatcctag actagacgaa aactgataga gcgattttat
     1621 cccgggcggt ccttgaccgc ccgttccggt ttatgccccc gccacttccc tatatcctaa
     1681 tacctggccg ccgtcgccga tatagactat taggatgttc tcctgttcct gcatcttagc
     1741 atggggatag aagtatattg ccagatattc acccttgtag ctgacatctt tattgtcctt
     1801 gtttttgcat aggacgtcgg tatccagatt gttttcatat aatattctag tttcctcgaa
     1861 tattagggtc ttaccacagg attttaagaa gtcggaactc ttcttgatta tttccagatc
     1921 cgacatattt aaaatggaga tggacttatg ctggatctcg gcatcggggg cttctgtact
     1981 accccagact gggtttgggt tattgctttt attacacccc gacactaaaa ggatcagaat
     2041 aaaaatgggt atgagggtat gacagaacct tttcattgtg ctcactctcc aaatcgacgt
     2101 tgaaatgaac attcaatggc ttattatcta ttgccgggca acgggtaacc agacggcgta
     2161 taggggcgat ttaaggcctt ggggctataa cggacaaaat ctttaggcga tactttatat
     2221 atatgctggg gaagcagggc tctattaccg ggatcgttga aagagtaacg gatgccatac
     2281 caaagtgtta ttcaacgggc atcccatact acatacaggc acaacgggtt atcataataa
     2341 attttatgaa ttatttacga agggaggcag cgcggttaca cgccgcctga tgacatgaag
     2401 gacttaagca aaggaaaact cccgcaacgg ggggaaatac cgaaggaatt tagatggaaa
     2461 ctgggggata tctataaatc cgcttcccat tgggaggacg actacagatg ggtcaaggaa
     2521 aatctaccgt cccttaaaga acatgaggga aacctggggg agtccgcaca gaacctgctc
     2581 agtgggctgg acacctatac gggacttatg aaaagggtgg aaaaactcta cgtatacgcc
     2641 catatgaaaa aggaccagga caatgccaat acccaaagcc aggggctatt ggaccgggcc
     2701 cagggcctga tggtggaatt tggaggtttc acatcctttc tggtgcccga aattttagag
     2761 ataccagaga gcaggattaa agagtatata gaggcccttg atggcctcat gccctacggc
     2821 cattatttag gggatataat gagaaggaaa gagcacgtat tatcccccaa ggaagaaaaa
     2881 atcttggcta tggccgggga agttgctgcg gccccggaac acatctttgg aatggtcaac
     2941 aacgccgata tgcgctttcc ctacataacc gacgagaacg gacatgaggt agaactgacc
     3001 catgggcgat acctgcagtt tatggaaagc agggacaggg aggtcagaaa acaagctttt
     3061 gatgagctct ataagaccta cggcaagcaa aagaacacca tagccgctac cttaaattat
     3121 tccattaaga aggatatttt ctacagcaag ataagaaact atccgtctac cctagagaac
     3181 gcacttcatt cggataaggt tccggtactg gtatatgata accttatcaa ggccgtagag
     3241 gataatatgg atgctatgca caggtatgta tccctgagaa aaagggccct ggggcttgat
     3301 gaactccata tgtatgacct ctatgtgccc attgtccggg atgttgagat gaaaatcccc
     3361 tataataagg caaaggatat gataggggag gcgctaaacc ccttagggga taattacctt
     3421 gatatcctag aggaaggctt taacagcggc tggatcgatg ttttggaaaa cgagggcaag
     3481 accagcggtg cctattcttg gggctgctac gatacccacc cctatgtgct gatgaactac
     3541 caggagaata tagacaacgt ctttaccctg gcccatgaga tgggacacgc cattcacagt
     3601 tatctatcca acaagaatca gccatatatc ctggcggggt acaaaatatt tgtcgctgag
     3661 gttgcctcaa cgctcaacga gatcttgttg accaaccatc tccttgaaaa cctggaggat
     3721 gaccggcaaa aggcctatgt cttaaactac tatctggaac agttcagggg aacggtatac
     3781 cgccagacca tgtttgctga atttgaaaga attgtccacg gtatggccga gggcggcgag
     3841 cccttaaccg tagagagcct gtcagaggcc tattataagt taaatcttaa gtactacggg
     3901 cccgatatgg tagtagatga tgagatagcc ttggaatggg ccaggattcc ccatttctac
     3961 tcgtccttct acgtatacaa atatgccact ggcttttcgg ccgcagcagc cctggcacag
     4021 aggatattgg atgagggcca gccggctgta gatcgctatc tgaatttcct atccaggggc
     4081 ggttcggatt atcccataga acttttaaaa ggcgcaggtg tagatatgac atcgccgaaa
     4141 cccgtaaatc aggccctcaa aaccttttct gcactggtgg ataggatggg gaaactatta
     4201 tagcagtaag gaaatgtata gcagtaaaga tatggggttg atcaagactt gatacgaaac
     4261 attctaaagt tcttgaaaat aataatattg gcaacgctga tagtactgct atgcgtagat
     4321 attgtcggag tatatctggg cctcatattg ggtaagacgg tactggggcc caggctcttg
     4381 attgagacct taaaaaggca tgaggcctac gcccagataa gacagctaat tttcaagctg
     4441 gtaaagggaa gccttccaaa cgggcgagac agtatcccat acctggaaaa ggcaatatcc
     4501 gaaagctggc tggaacagga gatcaatatg ctccttaaaa actattatgc ctttgccagg
     4561 ggcgaaagga aggatacccc cattatattc ttcagcggcc tgaaaagacg ggtagtggat
     4621 gccctggatg gggatcaacc ccacgggaac aggatcagat tggtaaagtt ttggttcgac
     4681 ccgttgcccg atgaggttcg gctggaggat tttatgtccg tagacctcct atggggtatt
     4741 cggggagcct ttgatcttat gctgcggatt ccgtggataa tgggcggtat agcccttttt
     4801 atcattctga ttatgcattt gctcttgctg gattggaggc agtcaatgct ctggtttggg
     4861 gcagctataa taacagccgg ggctttgttg atattaatgg gcatagctgc ggtatgggca
     4921 gctggacata tgagcatcct ggcaaacatg aaggacaggc tcctgtccct ggatatccca
     4981 aaggccaacg tagtcaattt tttcaattca tttcttaatg gcattatcaa tcccatgaac
     5041 ataatcggta ccctatcaat tctaacaggc gggatgttaa tatactttgt ccccatagcc
     5101 gaagagcctc ccaaaccggt gacggagtag ggaggatttt caattctgcc ctttgcatgt
     5161 attctaatga ctaaatttgc taatactcta taggacaagg gtttcatcaa gatgcaaaaa
     5221 aacatgaaaa cctttacccc ggtatatgtg gattgcttgg gtttccgggc tttatgagtc
     5281 ctggggaatt cttgacgtgc aaagaggagg agcataatgg aaaagccgga tgtgctttat
     5341 tcagaggcca gggatctagc cgaagaacat aagggaatag tctacagaaa atcggtgaac
     5401 agggattttc tcaaaataga acaggatagt cagatccttc ttgaagccta tagggatatc
     5461 aatgagcagg taaaggcaaa acaggacatt atacctgcgg cagaatggct tttagacaat
     5521 ttttatatgg tagaggaaca atccaaacag atacaacacg aactgcccaa taacttcagg
     5581 gaattgccca tattaaaatc gggaattcca agggtttatg aaatagcgga gggtatcgta
     5641 tcctttaggt cgggacaatt ggacgaagag cctataatag gatttttaaa ggaatatcag
     5701 tctgttacgc cgctgaacaa tggtgaacta tggatcctgc ccttgatgat caaggtttcc
     5761 ttgataaaga ggatcaggga aagagcggtg cacatagtag aatatcaaaa acagagaaac
     5821 cgagggcgca gatggggagc cctgttggtt gagaccatcg atgctcccgg ggacgagttg
     5881 caacgcctca ttatggagca cgatagggaa gtcgggtata tgctgccatc ctacgcagag
     5941 ggaatgctta gagtgtttcg cgacagtggg agcaagggag catcccttat gatgtggata
     6001 gacggaaagt tagcgctcca aggcaccaac tccgatgaac tgatacaagg ggaaaggcag
     6061 caccagagcg attgccaggt ctccatagga aactacataa taagtctaaa gtttttgttg
     6121 tccatcagat gggaagaaat ctttgaggaa ctgagcaacc tagacggcat cttaaacaag
     6181 gacccttcgg gcttttattc cgacatggaa tttacctccc ggaactacta cagaactgaa
     6241 cttgtgaaga tcgccaggag atatagggta agcgagctgg aagtggcaag gcttgctgtc
     6301 gatatcgctg aggataccat ggaacccaca gggggggata tcaggctggg gcatgtgggg
     6361 tattatcttg ttgaccaggg cagaagagaa ttaaaggaac ggctaggcgg caagcacccc
     6421 aaagaaggtt ggctcaagga gcatcccacg gccttctatc tagggtcaat cgggattatt
     6481 gtggccgtaa cgatatttct gatgctaaac tatctaagcg ggctgtcccc ggcacaaaat
     6541 ccgctaatat tggtgctctc tgggatagtt atctttatac ccgttgtaac cttggctgtg
     6601 ggtctgacgc actggatcat accaagaata ttcccgcctt gctacctgcc caagctggag
     6661 ctggaggatg gaatacccga agaataccgc accatggtga tcgttccgac ccttttgacc
     6721 agcgaaaaaa gggtttggga actcatagag cagatggagg tattctatct ggcgaaccag
     6781 ggagataaca tccattttgc cctggtgggg gactttaagg actccaagaa tgaaaaggaa
     6841 cccgatgatg aggccataat agatgcggct accaaggcgg tggaagaatt aaaccaaagg
     6901 tacagccggg gggacggggg catattctat ttcttccacc gtcataggca gtggaccccc
     6961 gcccaaaaat cgtggatggg gtgggatcgc aagagggggg ccctggtgga atttacggga
     7021 ctcctaaggg gaagggagga taccagcttc tcgacccaag taggggatat gtccatgctt
     7081 cccaagataa agtatgtaat caccctggat gcagatacca atctaccgag ggacgtggcc
     7141 aaaaggcttg tgggaaccct ggcgcatccc ctgaatcggc ccatagtcga taaatccctc
     7201 aacagggtag tagagggtta tggggtcttg cagcctagga taggggtttc agttgacagc
     7261 gccagtcgct cgtcctttgc tctgaccttt tccggacaga cgggggtgga tccctatacc
     7321 actgctgtat ctgatgtgta tcaggacctt ttctgtgaag ggatttttac gggtaaaggg
     7381 gcctacgatg tcgatgtatt caatgatata ctgagctctc aaataccaga gaatagcatc
     7441 ctgagccatg accttttgga aggctcctat ataagggcgg gcctcgttac cgacatcgag
     7501 ctgatagacg gttacccggc caactatatc tcatatgcca tgcggtccca taggtgggta
     7561 aggggggatt ggcagctctt gccctggctc tttggtaagg tgaaaaatgc caagggggag
     7621 aggataagaa accccctttc ggcaatctca aagtggaaga tattcgataa cctgagaagg
     7681 agtcttttat ccccgtccct ctttttgctg ttgaccctag gattgaccgt acttccgaga
     7741 tccgactggt tctggttggg gttattcacc ataacgctga cgctgcccct gcttatggat
     7801 gtggtagcct cctttatagg cagggtgggt tcgccgaacc ggttcgccgg ggatatcctg
     7861 tacggaacaa agaatttggc catgcagata atcctgacct ttgtcttttt ggcccaccag
     7921 gcatatctga tgacggatgc cgccataaga tccgttgtga gggtgttttt taccagaaaa
     7981 aacttgctgg aatgggtcac cgccgcagat accgacagaa ggtttaaggg aaccctggag
     8041 gactattgga agaagatgct gccggctatg ctgatctccg tggctttttt tatctgggtg
     8101 gtgaccttta gaagatcctt tctatttata cccctagcca tatccctcat ttggctaata
     8161 tcgcctttaa ttgcctacag gatcagtcag cccaaataca gaagggaatc cgacctgtcc
     8221 aaagagcagg tacatagatt gcgcatgata ggcagaagga cctggaagta cttcgacgaa
     8281 tttgtaggcg ccggggataa ttggctcccc ccggataact atcaggaaga gccccatgtg
     8341 gggatcgctc acaggacctc gcctaccaat ataggcctgg cccttatatc tacattgact
     8401 gcaagggatc tggggtacat aagcacaacc caggcaattg aaaggtttga aaacaccttt
     8461 aggacgctaa ggcggcttga aaaatggaac ggacattatt ataactggta taacacccgt
     8521 accctagagc ccctaaagcc aagatatgta tcctctgtgg acagtggaaa tctggcctgc
     8581 taccttatcc tgctcaagca ggggatagcc gagctgttgt caaggcccct ggtaggcaag
     8641 gaagtggccc tcggtataag agataccatg gacttagact ttgaaaagag cggaagatcc
     8701 atacccccca tcataaatat gttagaggcc agcgaaaaca ttactctgac ggaatggaag
     8761 ctgaccttga accaattggg aaatatcggg aattacaccg acaggcagat acatcagtac
     8821 agggaagaga tggccgccat ggtgccttgg gttgacctac tattggacgt tcctaggagc
     8881 attttagggg agaatgggct tcctgccgag gcaaaggaga gctttggaat cctcatggat
     8941 aagctgaaca aggaattttc cctaaagaaa atcctagacg attatcatta tgtcttggat
     9001 aaattatcga ggataatcct gtccttaagg aggacaaaaa acccggacaa ggagtccaac
     9061 ggggtggcta catggctaaa gcagctggag attgccctgg gtgaatccca tatggccgcc
     9121 agaaagatgg cccataggtg caaaaagctt atggacatca tagacgaaat atttcaggat
     9181 atggatttcg ctaccctata cgataaaaag aaggagttat tctccattgg atacgacatt
     9241 gaagcggggg agctgcagga tgtatactac gacctattgg cctccgaggc caggcagacc
     9301 agctttgtag ccattgccaa gggggacgtc ccacaaaagc attggtttaa gctggccaga
     9361 tccttgacta tggtaggcga ttccaggagc ctcttgtcct ggggcgggac catgttcgaa
     9421 tacttgatgc ccatgcttat aatgaaaaac tataggaata ccctatggga cgaaacatac
     9481 caggcggtct taaaggggca gaggcagtat agcgatcaga ggcatctacc ttggggagtc
     9541 tccgaatccg ccttctatgc ctttgacctt cagctaaact atcaatacaa agccttcggg
     9601 gtgcccaagt tgggccttaa gaaggggctt ataaaggata tggtggtcgc accctatgct
     9661 tccataatgg ggcttaacat agatccccta gcggttatga agaacattga attcctggaa
     9721 tcggaagggg ccagcggcaa atatggaatg tatgaatcca tcgattatac gcccgaaagg
     9781 gtccccagca gaaaaaagag catgctggtc aagacctata tggcccacca tcaggggatg
     9841 atactcctgg cctttaataa ttatttgaac aactgcgtta tgcaggagcg cttccatgcc
     9901 atacccatga ttagggccac ggaactgttg ctacaagagc ggatccccca aagggaaatt
     9961 gtcatcaagg agcacagcga cgcctttgat attccggata tggacacgga gatgaaatcc
    10021 tatcaggaaa tccatgcaag aaatatcata gatacggccc atactcccgc gccggaggtt
    10081 tgtgtgctgt ccaacaacta ctataccacc atagtcacca acagcggcgg cggattcagc
    10141 caatatcagg agctggctat taatcgctgg cgggaggatg taacccggga taattgggga
    10201 atgttcttct atattaaaaa cttgaactcc gggaactact ggtcatcggg atatcagccc
    10261 tgtgaggact ccggagaaaa gtacgaggtc gtctttgaag gtgataaggc catcttttca
    10321 cgaaaggatg gcaatattga aacaaagacg gagataacgg tatcgccgga gcatagcgtt
    10381 gagattcgga gaattacact gaacaatcac agtgagcacg aaagggcgat ggaggtcacc
    10441 agctattttg aacccatcct tacgctacag ggggacgata ccgcccatcc ggcatttgtc
    10501 aaccttttcg ttgagaccga atatgtccca gaacacgata tattggtcat gacccggcgg
    10561 cccaggagca aggactacaa gcagatgtgg ctggcccata ccatgatagc ggagggagaa
    10621 cccatagggg ttgtgcagta cgaatcggat agatcaaaat tcatcggccg aaaccggggg
    10681 cttaaacacc ctgtagccat ggaccccgat caacccctca gcaataccgt gggagcggtg
    10741 cttgaccctg ttatgtgcct gagacagcgc attggcatag ggcccggcca ggcagccaag
    10801 ctatcgtaca tggttgccgt tgccgatacc agggacgagg ctattaacct ggctagggaa
    10861 tatcgaaata cggccgttat atccagggca tttgagcttg catggaccca tagccaggtg
    10921 gagatgagat atctgaatat ctcgtctaca gaggtgaacc tgtaccagaa gatgctctct
    10981 tccataatat atacaaaccc tgccagaagg gccatcctaa aggatattat gaacgaagag
    11041 gaccaaagcc ggctctggac atacggtgtt tccggagacc ttcccatagc gaccatcagg
    11101 ataaccaaca ccgaacagct ggaaatggta aggcagatgc tgtccatcca cgaatactgg
    11161 cggctgaagg ggatactagt ggacctggta atactgaatg agtacggcaa cagctacgaa
    11221 cagcccgtcc aggaccgtct gagggagatg cttatggtca gccacttgag ggagctgcag
    11281 gacaaaccgg ggggagtatt cctcctggca ggtaacttga tgcccgatgg ggataagaac
    11341 ctgttggcct atgcttccag gattacaata gacggcgaga tggggtctgt ggcctctcag
    11401 ttagaggcta atggctcggg cgataagctg ccggacgcaa tggagtatgt aggctctgag
    11461 aagacggaag aagatgagat aaaggagtat ggggaactct tatactttaa caatatcggc
    11521 ggatttacgc cggacgggag agaatatgag ataaggctcc aaggcggcaa cgccaccccc
    11581 cttccctgga gcaatgtcgt agccaatgag gtattgggat ttttggtaac ggaatccggg
    11641 gcgggatata catggtacgg taacagccgg gagaataaac tgacgccctg gtccaacgac
    11701 cccgtcatgg accctgcagg tgaggcggta tatgtaaggg atgaggattc cggggctgtc
    11761 tggtccattg cgccgactcc gattcgcacc gacggggact ataccatacg ccacggtcag
    11821 ggctattccg tcttcgaata cgacagaaac agattgagac aaaagcagac catgttcgta
    11881 gttcccgacc agcccattaa gtactaccat atagggctta cgaacgattc ccaaaaggta
    11941 agaaagctca ctataacctt ctacgtcgaa tgggtatgcg gcgtcaaccc catccccaac
    12001 aaccaattta tcgtgaccgg tgaagacagg gctataaacg ctatccttgc ctacaacccc
    12061 tataacggtg aatttggaaa cagggtagcc tttatagcct gcgacaaacc ctatacgagc
    12121 attaccggag acaggacgga atttatagga aggaacggat ccctaaggga tccggctgcc
    12181 ctggggcggg tggggttgtc caaccgggtg ggacccgggc ttgacccctg cggtgttatt
    12241 caagtggccc tggagattgg acccggggag ggagaggaac tggtctttgt attgggccag
    12301 ggagacgatg aggcccaggt aaggcagctc ataggggatt ataccaatat ggccgccgcc
    12361 caatcggcct tggaaggggc a
//
LOCUS       DASRNX010000238        14429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_732893,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000238 DASRNX010000000
VERSION     DASRNX010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 14429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14429
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732893"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1225)
                     /gene="tuf"
                     /locus_tag="VFD89_09485"
     CDS             complement(8..1225)
                     /gene="tuf"
                     /locus_tag="VFD89_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZJ58450.1"
                     /translation="MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITMVLSKLGQSEA
                     AKYDEIDKAPEEKERGITINTSHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIIVFMNKTDMVDDEELLELVEMEVRELLSE
                     YEFPGDDIPVVMGSALNALETVLNDESVDVNENEACKPILELMDAVDSYIPTPERDVD
                     RPFLMPVEDVFSITGRGTVATGRVERGTVKVSENVQIVGLSDEPRTVVVTGVEMFRKL
                     LDQAVAGDNIGVLLRGVQRADIERGQVLAKPGSINPHTKFMAQVYVLTKEEGGRHTPF
                     FNGYRPQFYFRTTDVTGVAHLPEGVEMVMPGDNIDMEVELITPIAIEEGLRFAIREGG
                     RTVGAGVVASVAK"
     gene            complement(1323..1398)
                     /locus_tag="VFD89_09490"
     tRNA            complement(1323..1398)
                     /locus_tag="VFD89_09490"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1363..1365),aa:Thr,seq:ggt)
     gene            complement(1517..1590)
                     /locus_tag="VFD89_09495"
     tRNA            complement(1517..1590)
                     /locus_tag="VFD89_09495"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Gly,seq:ccc)
     gene            complement(1730..2371)
                     /gene="sigH"
                     /locus_tag="VFD89_09500"
     CDS             complement(1730..2371)
                     /gene="sigH"
                     /locus_tag="VFD89_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HZJ58451.1"
                     /translation="MEASTASRKTGNYDILSDEDIVIKALGGDTYAYDYLLEKYKNFV
                     RAKARSYFLIGADREDIVQEGMIGLYKAIRDFQPEKLTSFRAFAELCITRQIITAIKT
                     ATRQKHIPLNSYISLNKPIYEEESDRTLLDVITGARATDPEELIIRKEEFTNIETVIE
                     KLLSDLEWEVLTSYLQGKSYQEIALELDRHVKSIDNALQRVKRKVERYLEERD"
     gene            complement(2506..3273)
                     /gene="rlmB"
                     /locus_tag="VFD89_09505"
     CDS             complement(2506..3273)
                     /gene="rlmB"
                     /locus_tag="VFD89_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZJ58452.1"
                     /translation="MGESGSGRDGDNEGQVEGRNPVLEAFRSGRTVEKLLVAQGAREG
                     SILEIIQKARAQGIVIQEVDRRRLNELSMTDGAHQGVIAFVTPYSYVGIEDILARASQ
                     LGEEPFIIILDEITDPYNLGSVIRTAECCGAHGVIIPKRRAVGLTPIAIKASAGAVEF
                     MPVAKVTNISDTLELLKKHGLWVAGADMDGDSYTSKDLTGPIALVVGSEGRGLGRLVK
                     EKCDFLVKIPLMGRISSLNASVAAAVLMYEIARQRDG"
     gene            complement(3524..3940)
                     /locus_tag="VFD89_09510"
     CDS             complement(3524..3940)
                     /locus_tag="VFD89_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HZJ58453.1"
                     /translation="MGHLKELIGKDLGEDEIKMMNPLVLAYIGDTIYDLLVRTYLIIT
                     EDLTVHSLHNRAINYVSAGAQAHTLEDILGRLTEEEKNIIRRGRNTKSGTVPKNADIG
                     EYKHATGFEALLGYLYLTERTDRLMEIGGWILKSHR"
     gene            4173..4481
                     /locus_tag="VFD89_09515"
     CDS             4173..4481
                     /locus_tag="VFD89_09515"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZJ58454.1"
                     /translation="MKGCYGWRNLFNPANMHGRRWFAGMMPMHSWGGGLFMFLFWALI
                     IAGVVYLIIRLSRNSSPSRYMPVGGQDNAINILRERLARGEISQDEYDERLKILKEQP
                     "
     gene            complement(4643..5494)
                     /locus_tag="VFD89_09520"
     CDS             complement(4643..5494)
                     /locus_tag="VFD89_09520"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZJ58455.1"
                     /translation="MLKKWSVVLLVAMAIIALFSILNPGGANNSAGIVSASDPDTNIG
                     AYRVKNGDSLYLIAAENGTTVNDLMEDNGLESDLILPGQDLIIDENSIDRERINRQSV
                     EYENIEQRDIDSKSTGQDNMDEIRVVIEKTHQKLSLYRGDTEVKVYKAHFGEAGMEDK
                     EIQGDRKTPEGTFYITEKSVLTPADEYLGSRWLRLSYPNVEDARRGLEQGIIDRITHD
                     SIAEAIGRGETPPQRTALGGGIGIHGGDVPEFGDNWTWGCIGLTNEDAEEIYEYVTVG
                     TPVVITR"
     gene            complement(5995..7164)
                     /locus_tag="VFD89_09525"
     CDS             complement(5995..7164)
                     /locus_tag="VFD89_09525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZJ58456.1"
                     /translation="MEVKFIENEHKESIKHILNCCFEMPPDMVEGIVSKDFNSEDWLG
                     CFDGDKLAGLIYISSHDMYFYGKTVPMAGIGVVSTLPEYRNRHCASGILIKSLEVLKD
                     RGYVFSALGPFSYSFYRKYGWELGFHTKKYTISMDGLKGIGTGKGEFRPLRPRDIDNI
                     VGVHGNFFSRYNGAVKRDNKRWQSRINGLGKDRTYGYAYSHQGNGLAGYIIYSVKEWT
                     FTILEMAYDSLETKLEILRFVYYHSAQAERVVWEAPQDDNTALILANPPTEQKIRPGM
                     MIRVIDVKAALEAYAYPILYKGSFTIKIDDRWAPWNSRVLRVIIGEGVATVEDAGGDP
                     ADLECDIQTFSQIISGYIGMGEAKELGRVTAHNQEILEDTEIIFRKHVTHMTEGF"
     gene            complement(7378..8604)
                     /locus_tag="VFD89_09530"
     CDS             complement(7378..8604)
                     /locus_tag="VFD89_09530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZJ58457.1"
                     /translation="MNRRGRALQYIALRKPFVRLLIGQGIMEFGDNFRLLAVTILLLE
                     TTGSGLAAGFSLVITPIVGILLSPFAGVLGDRLNGAKFLSGLYLLQGVLATLFIHKIQ
                     LHMVYTILMFFACIQIVQEPVFRKAIRDSLRGGDALVGNSLSMGMSGLSNLLGPVLGG
                     IAVHLWDKERVLMASTLCYGTSALIVLFIKDIYRRSAVNDIRFRPGGFLNEIGDGIKY
                     FWRMDSIRELGIAGFVLSFGTTSVSFAFYSLAFDKMGVDAKLWGFILSVFYSTRLLAM
                     FISVIVDRRGGVKGAKTFYLPLFFTCLSWLMYGFSKNLSSVIIALIIEGTAHFLFDIS
                     LWTRLQTIAKGTLMARVFGINNIMSNTARVMGIILAFILIRFFDITVVFMVNGAIIFV
                     YAFFKAILNKPGHISY"
     gene            complement(8691..9731)
                     /locus_tag="VFD89_09535"
     CDS             complement(8691..9731)
                     /locus_tag="VFD89_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HZJ58458.1"
                     /translation="MGYPDYRYNYYFKGGGHIFSRKSLITILITLVIISVLFALNPNP
                     PKSADHRSGRRGDGEIKGPKSKGPQLWDNGTGAPGKGGGGHIEIRVVKDEKSGQVETL
                     PWERDTDFLKAGNEHGTKVLMAAYRTVLPDPLPGEEYNVHLGARLLSGKIIEPGKIFS
                     QNREIGPYDESKGFKSGPTYAGTQLITTIGGGVCKISSTLYNVAVLSNLEIVQRYNHS
                     MPVPYVPYGQDATVAYGSRDFQFKNNTSFPILIWAEGIENMLYIAFYGEEKPPDIKWH
                     HDVTDIQKAPILYKKNPELSPGVEHRVVDGMDGATVKSGVTVSNPDGTVEIKDMGTSY
                     YRPMPSIIERND"
     gene            complement(9789..10364)
                     /locus_tag="VFD89_09540"
     CDS             complement(9789..10364)
                     /locus_tag="VFD89_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZJ58459.1"
                     /translation="MELIEGVILAAGLSKRTGVCKMLLDLGGITLIERCITGMYRFCS
                     KIVVVGGHNFENIGRVIAKYQKVRMVYNPHYREGMFSSAKEGFKNVSGDRIFFIPGDY
                     PLVTGETYGKMLGAEGDIIVPLFNGENGHPILIRGHLVEELLAEGRYSTLREFISDKG
                     YIPVPVRDPGILKDIDTMEDYDRVKESIPGL"
     gene            complement(10368..10793)
                     /locus_tag="VFD89_09545"
     CDS             complement(10368..10793)
                     /locus_tag="VFD89_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58460.1"
                     /translation="MAVLAACIKLLDFFIPGHDPIRIIYPAMAIVLEGMAVYGGFILA
                     EKRDRGFGYLEALFVSLSWRMVFTAVQFMTLPQSQVPTLQSLMKFFLVDSPINSGLIY
                     LYIRFIRSREKVEGEMITRINPIAAVVTLALSILSKWVI"
     gene            complement(10904..11665)
                     /locus_tag="VFD89_09550"
     CDS             complement(10904..11665)
                     /locus_tag="VFD89_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZJ58461.1"
                     /translation="MIPFDFEYYRPDNISQAVDLFMGIAGQGKKTMYYGGGTEIISMA
                     RTNSIYVDAVIDIKGIPECNVLGYAETDLVIGAATTLTKLSESNLFPMLGITVARIAD
                     HTLQGKITLGGNISGTIMYREASLPLLIADCRVSIAGPGGTRETPLSQIFDGRTKLEE
                     GEFITQFMMDSSNTSLPFFHRKTTKIDKIDYPLITVVALKKDNRILAGFTGLCNFPFR
                     SLEMEDSLNQGGISKGDRIQNALGFIPAPVAIYCI"
     gene            complement(11662..11877)
                     /locus_tag="VFD89_09555"
     CDS             complement(11662..11877)
                     /locus_tag="VFD89_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58462.1"
                     /translation="MRFGQQPDYLVEFIETPAENGPYGARGFGEHGILGMPAALANAL
                     TRAAELELDQVPIDPELIWKMKTGALS"
     gene            complement(11904..12581)
                     /locus_tag="VFD89_09560"
     CDS             complement(11904..12581)
                     /locus_tag="VFD89_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZJ58463.1"
                     /translation="MGTTVPRKEGLDKVTGAAKYNDDIREPGLLHVKMVTSTCSHGLI
                     KSIDVTKAWKVPGVRAVVTGDYLPVKTGSVIEDRPPIAVDKVRYFGEPVAIVVADTEH
                     EAQLAAYSINIEYEPLPVVKGPAQAIEAGAPLIHENLGQYKKAIDDVYPVANTNIADH
                     TQIRKGDMKRGWEESDVVVEGEYFMPKSDHIAMETRNVRVKIDPTGKVRVSGAGKPLY
                     TAATGAY"
     gene            12882..13082
                     /locus_tag="VFD89_09565"
     CDS             12882..13082
                     /locus_tag="VFD89_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZJ58464.1"
                     /translation="MQGTVKWFNSEKGFGFIVSEEGEDVFVHFSAIQTEGYKTLEEGQ
                     RVEFEIEQSPRGPQAVNVVPSY"
     gene            complement(13150..>14429)
                     /locus_tag="VFD89_09570"
     CDS             complement(13150..>14429)
                     /locus_tag="VFD89_09570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZJ58465.1"
                     /translation="IELINSPNRRNQDFFIIQVIFANAFAILTGGEFLSGFAIFLGAS
                     DELVGYIPLIGGISGISLIFFGILMERFTKRRKLVLTLNSIVKPLLISIILIPLLVPK
                     PAQVLVLFILLFVVYILNAFLGLAVNSWFVKAIPIKLRGRYFSIRQIYAVLVMVLLPI
                     VAGGILDTTRSRYLGFIILFAAAFVFGMGETYAFSKVEDAQVEGIGKGIKILDVFRIP
                     IKNKEFMDYTVINVLFHIALYLSASYTQVYMIRYLELSYTFITSMTMLNAVLQMFAYS
                     QWGKIGDRYGNVFVMYLSYGFYAMQMALWALVTKGSMYVFIPLVYGIGSFANSGFMVG
                     SFNYRYDIMPEKGRSFYDAFYSAAIGITLLIAPWIGGRIKGFIAGIPYLNDNIMLGEF
                     RIIFALSALGLAVLVLYIVLKRRKANTNQGISI"
BASE COUNT         3630 a         3740 c         2995 g         4064 t
ORIGIN      
        1 tcaatattta ctttgcaacg ctggctacaa cgccggcacc cacggtcctg ccgccttcac
       61 ggatagcgaa ccttaggccc tcttcaatgg cgatgggggt gataagttcc acttccatgt
      121 cgatgttgtc cccgggcatt accatctcta cgccttccgg cagatgggcc acacctgtta
      181 cgtcggtggt cctgaagtaa aactgaggtc tgtaaccgtt aaagaaaggg gtatgcctgc
      241 cgccctcttc cttggtcagt acataaacct gggccatgaa cttggtatgg gggttgatgc
      301 tgccgggctt tgcaagaacc tgacctctct cgatatcggc cctttgaacg ccgcgaagca
      361 gtacacctat gttgtcgcct gcaaccgctt ggtcaagaag ctttctgaac atctcgactc
      421 ccgttacgac tacggtcctg ggctcgtcgg aaagacctac gatctgtacg ttttccgata
      481 ccttgacggt tccacgctct accctgccgg tggcaactgt accacgcccg gttatggaga
      541 atacgtcctc aacgggcatc aggaagggtc tgtctacgtc cctctcaggt gtggggatat
      601 aggagtctac ggcatccata agctctagga tgggtttgca tgcctcattc tcgttaacgt
      661 ctacggattc gtcgtttaga acggtttcta gggcgttaag ggcagaaccc ataactacgg
      721 gaatatcgtc tcccgggaac tcatactcgc ttaaaagttc tctgacttcc atctcaacca
      781 gctctaagag ctcttcgtcg tctaccatat ccgtcttgtt cataaatacg atgatgtagg
      841 gtacgccaac ctgacgggca aggaggatat gctccctggt ctgaggcatg gggccgtcgg
      901 ctgcggatac aaccaaaata gctccgtcca tctgggctgc cccagtgatc atgttcttta
      961 catagtcagc gtgaccggga cagtcaacgt gggcatagtg acggttttcc gtctcatatt
     1021 ccacgtggga cgtgttgatg gtgatacccc gttccttctc ttcgggggcc ttgtctatct
     1081 cgtcatactt tgctgcttcg gattgcccca gcttggacaa taccatggta atggccgcag
     1141 tcaaggtggt cttgccatgg tcaacgtgcc ctatggttcc tatatttaca tggggctttg
     1201 ttctttcaaa ttttgctttc gccattttcc ttcttcctcc ttcgtgaccc ggcaacactt
     1261 attattatgt aaccgctaat tacacatagc caggtgtttc atttatattg attatatagc
     1321 aatggagccc acgaccggac ttgaaccggt gacctccgcc ttaccatggc ggcgctctac
     1381 ctgctgagct acatgggctt attagttact agattccggg caccgggtgg tcaggccgag
     1441 atcagcccct ggcttattct aaatggaatt tcttccactt agcttatttg ccccctagtc
     1501 ccctatctct agaatatgga gcgggtgatg ggaatcgaac ccacgtagcc agcttgggaa
     1561 gctggtattc taccattgaa ctacacccgc atattgtcaa tttgttttac cgcctcatgc
     1621 aaggcttagc atgcccgtga aactccttgc ttctataaaa tagttatgac gaacacgtta
     1681 tatattataa cttttcgccg cttttgtcaa ctgctttttt gaaacttttt cagtcccttt
     1741 cctccagata acgttccacc tttctcttta cacgttggag cgcattatct atggatttta
     1801 catgcctgtc caattcaagg gcaatctcct gatatgactt tccctgaagg taggatgtta
     1861 ggacctccca ttcaagatcg ctaaggagtt tttcgataac ggtttctata tttgtaaact
     1921 cctctttcct gataatgagt tcctcggggt ccgtggccct ggcacctgtt attacatcca
     1981 gaagtgttct gtcggattct tcttcatata tgggtttatt cagggagata tacgagttaa
     2041 ggggtatatg cttttggcgt gtagccgtct taatagcggt gatgatctgc cgtgtaatgc
     2101 atagttctgc aaaggcacgg aaggatgtta gtttttccgg ttggaaatcc cgaatcgcct
     2161 tgtatagtcc gatcattccc tcctgaacta tatcctcgcg gtctgcgcct attaggaaat
     2221 aggaccgggc cttggccctc acaaagttct tatacttttc caaaagatag tcataagcat
     2281 aggtatcacc gcccaaagcc ttaataacta tatcctcatc cgataaaata tcataattcc
     2341 ctgttttcct actggcagtg cttgcctcca ccgaaatccc cccataaata tgccaggtcc
     2401 tcgcagctat gttaagccta tgacccattc aattatacat tacataatcc cggcaagtca
     2461 agccgtgttt gcccagtttt cgcccataat ttccctaata tttcattatc cgtctctctg
     2521 tctggctatc tcatacatca gtactgccgc agcaacggaa gcatttaaag aggatatccg
     2581 gcccatcagc ggaatcttta caagaaaatc gcacttttct ttgaccaagc gtcccagtcc
     2641 cctgccttca cttcccacta ccagggctat gggccccgtg agatccttcg aggtgtacga
     2701 atccccgtcc atatctgcac cggcaaccca taggccatgt ttttttaaaa gctctaatgt
     2761 gtctgaaata ttggtaacct tggctaccgg catgaactca acggctccgg cggaagcctt
     2821 tatggcaatg ggggtgagcc ccacggcccg ccgcttcggt atgattaccc catgtgcacc
     2881 gcagcattct gcggtcctta tcaccgaacc taagttatac gggtccgtta tctcgtctag
     2941 gataattata aagggttctt cccccagctg ggacgcccgt gccaaaatat cttcgattcc
     3001 gacatagctg taaggggtca caaaggctat aaccccctgg tgagcaccgt cggtcatgga
     3061 cagttcgtta agcctccttc tgtcaacctc ctgtatgact attccctggg cccttgcctt
     3121 ttgaattatc tccagaatgg agccctccct ggcaccttga gctaccagca gtttttccac
     3181 tgtcctgccc gatcgaaacg cctccaacac cgggttacgg ccctccacct ggccctcgtt
     3241 gtcgccgtct cggccgcttc cagattcccc catggttctc tcatccgagg caccgccacg
     3301 atacctttca tttcggtcac ccgcttttct gttaccctta aacccggatc ccggtttttt
     3361 atccctattg ttcatgataa tcttctcctt aaaatcttct ctttaaataa ttccctttaa
     3421 ataattcttt tgtccctgtt cattgtcctt ctgaccggtg tggccatgag ctattattgg
     3481 tagcgtatga cctcaattac atcatacaat gggttaaata tgtttatcta tgggatttta
     3541 gtatccatcc gcctatttcc atcagccgat ctgtgcgctc cgtcagatac aaatatccca
     3601 ataaagcctc gaaacccgtt gcatgtttat attcgcctat atccgcattt ttcggtaccg
     3661 tcccggactt cgtgtttctc cccctgcgga ttatattttt ttcttcctcc gtcaagcgcc
     3721 ccaatatatc ctctaaggta tgggcctggg cgccggcgct tacatagtta atagccctgt
     3781 tgtgaaggct atgtaccgta aggtcctcgg tgatgatcaa atatgttctg accaataagt
     3841 cgtatatggt atccccgata tatgccaata ccagcgggtt catcatcttt atttcatcct
     3901 cgcctagatc cttccctata agttccttta aatgtcccat aatcattcca taacaacctc
     3961 catagtatta tacccattgt acaccatatt ccgccgcctg tcccctgact tgcaccaaag
     4021 tttgggccga tattatttgt attaaagggg gatctgggat aaaccatact gctacggccg
     4081 gatgacaatc gtttgattat tttctgaata aagggttata tatatgaata gaagcatatg
     4141 gtagcagatt attttaacgg aggaggatga ccatgaaggg atgctacgga tggcgaaact
     4201 tatttaatcc ggctaatatg cacggcagaa gatggtttgc cggcatgatg cccatgcaca
     4261 gttggggtgg cggccttttt atgtttcttt tctgggccct tataatcgcg ggagttgtct
     4321 atcttatcat tcggctgtca cgaaatagtt ccccctcgag atacatgccc gtcggcggac
     4381 aggataatgc cataaacatt cttcgggaac gcttggcacg gggtgaaata agccaggatg
     4441 aatacgatga aaggctaaag atattaaagg agcaaccttg atttccttaa ccatactata
     4501 aaggggccgt tacgccggcg aacagttaac accggtgtga cagccccttt tttttaaagg
     4561 attgactcac catatatttt atatttttca ggcgtccatg aaacgcccag gctttcagac
     4621 agcagtcaga gatataccaa aatcaccttg ttattacaac aggcgttccg acggttacat
     4681 actcataaat ctcttccgca tcctcgttgg tcagacctat acagccccag gtccagttgt
     4741 cgccgaactc gggaacatcc ccgccatgga tgcctattcc tccacccaac gctgtgcgct
     4801 gcggcggcgt ttcccccctg ccgatagcct ctgctatgct gtcgtgggtt atcctatcga
     4861 taatcccctg ttccaatccc cgccgggcat cctcgacgtt tggatagctg agtctcagcc
     4921 acctggaacc cagatattcg tctgccggtg tcaataccga cttttccgtg atatagaatg
     4981 tgccctcagg ggtcttccga tcgccttgga tctctttatc ctccatccca gcttcaccga
     5041 aatgggcctt gtacacttta acctcggtat cgcccctgta gagggatagt ttctggtggg
     5101 ttttttctat tacaacccta atctcgtcca tattatcctg gcccgtgctc tttgagtcta
     5161 tgtcccgctg ttctatattt tcatattcta cactttgtct atttattctt tcccggtcta
     5221 tactgttctc atctataatc aggtcttgtc cgggaagaat cagatcggat tcgagtccgt
     5281 tatcttccat aagatcgtta acagtggtgc cgttttccgc ggctatgagg tatagactgt
     5341 caccgttttt gaccctatag gcgccgatgt ttgtatctgg gtccgaagct gaaactattc
     5401 ctgcgctgtt attagccccg cccggattta atatcgaaaa aagagcaatt atcgccatag
     5461 cgactaatag aacaaccgac cactttttta acatcccctt ttccccctta catgaaaaac
     5521 ggcagcatgt ttgcccttgt caaacttccg ccgcttcttt tgttttgtta aaacatcgga
     5581 agcaattgca ttggcaattg caacacacag gtttcaacga ggcgagggat ttatacccac
     5641 cgcctgtttt actaatcgga actcgccgct tatagaagcg ggagacatct tgcctactcg
     5701 ttatatatga attaagggca atgttttgcg acatccgtat accaatgcag aatattcgtc
     5761 gcaaagcatg tagtttacat aatatattat aacatggggt ttaagtaatt gccaaaggca
     5821 ataagtggtc ccattgccta attgatctag ccgattctaa ggaataatcc tctgtccctt
     5881 ggggagcaca gattggctga agaagttttt taatccgcga atccctaatg aacgcccggt
     5941 tgccgtcatt cgtacttgaa atctatgaaa cgacccaaaa tatttcttat acctttaaaa
     6001 gccctccgtc atatgggtaa cgtgctttct gaatattatc tccgtatcct ccagaatctc
     6061 ctggttatgg gcggtaaccc tacctagttc cttggcttcc cccataccta tatacccgga
     6121 aattatttgg gaaaaggttt gaatatcgca ttctaaatcc gcagggtcgc cgcctgcatc
     6181 ctctaccgtc gccacacctt cccctatgat caccctcaga actctgctgt tccatggtgc
     6241 ccatctatca tctatcttga tggtgaaaga ccccttgtat agtatgggat acgcataggc
     6301 ttcaagagcg gcctttacgt ctatcaccct tatcatcatg cccggtcgga ttttttgttc
     6361 tgttggagga ttagctagga tcaatgcggt gttgtcatcc tggggtgcct cccatactac
     6421 cctttctgcc tgtgcgctat ggtagtagac gaaacgcaga atctccagct tggtttccaa
     6481 agaatcatat gccatctcca aaatagtaaa tgtccattcc tttacggaat agattatgta
     6541 tcctgccaac ccatttccct gatggctata ggcatacccg tatgtgcggt ctttccccag
     6601 gccgttgatt ctagattgcc acctcttatt gtcccttttt actgccccat tatatctgga
     6661 gaaaaaattt ccatgcactc ctacgatgtt gtctatgtcc ctgggcctga ggggacgaaa
     6721 ttcgcccttg cctgtgccta tcccctttaa cccatccatg gagatagtat atttctttgt
     6781 gtgaaagccc aattcccaac cgtattttct gtaaaacgaa tacgaaaatg ggcccaaggc
     6841 ggaaaatacg tatcccctat ccttcaaaac ctccagtgat ttaatcaata taccggatgc
     6901 acaatggcgg ttcctatatt ccggaagggt cgataccacc ccaattccgg ccatgggcac
     6961 ggtttttccg tagaaataca tatcatggga tgaaatgtat ataagccctg ccagcttgtc
     7021 cccatcaaaa caacccagcc aatcctccga attaaaatcc ttacttacga taccctccac
     7081 catatccggc ggcatttcga agcaacagtt taaaatatgc ttgatcgatt ctttgtgttc
     7141 attttctata aattttactt ccaattcata tacccccaat aaggaatttt cagaatatac
     7201 ctaaatatat aatgaacaga tggtgttgtc aaacaagtaa cagataaaag gcgtataatc
     7261 taaatgtgaa gagtacaaaa gacacgtctg cagataaaca atacggaaac caaatatatc
     7321 tatagtccat atattatata gttgaagctg ccctccgcat cctgttttcc catagcttta
     7381 ataagagata tgacccggct tattcaatat ggccttaaaa aacgcatata caaagattat
     7441 tgcgccgttg accataaata cgacggtgat atcaaaaaac cttatgagta taaaggctaa
     7501 gattatgccc atcaccctgg cggtgttgct cattatatta ttaatcccga aaaccctggc
     7561 cataagggtc cccttggcta tggtctgcag cctggtccaa agtgagatgt cgaagagaaa
     7621 atgagcggta ccctcgatga tcaatgcgat aataacgctc gacaagttct tggaaaagcc
     7681 atacatcagc caggacaggc aagtgaaaaa aagcggtaaa tagaaggttt tagcaccctt
     7741 taccccgcct ctgcggtcaa ctatcacgga tatgaacatt gccagaagcc tcgtgctgta
     7801 gaaaacagac aggatgaacc cccatagttt ggcgtccacg cccatcttat caaatgccag
     7861 ggaataaaag gcgaaactca ccgatgtggt tccgaaggag agtacaaatc cggcgatccc
     7921 caattccctg atggaatcca ttctccagaa gtatttgatt ccatccccta tttcatttaa
     7981 aaatcctccc ggccgaaatc gaatatcatt tacagcggaa cgcctataaa tatcttttat
     8041 aaatagaact attaaggccg aggtcccgta acacagggtg ctggccatga gtacccgctc
     8101 cttatcccat agatgaacgg ctattccccc aagtacgggt cctaggagat tggatagacc
     8161 ggacatgccc atggacagag agttaccgac aagggcatcg cccccccgca gggaatctct
     8221 aatcgccttc ctaaaaaccg gttcctgtac gatctggata catgcaaaaa acatcaatat
     8281 cgtgtagacc atatgcagct gaattttgtg aatgaacagg gtggccaaca caccttgcaa
     8341 aaggtagagt cctgacagaa actttgcgcc gtttagcctg tcacccagca cccctgcgaa
     8401 gggggacagg aggataccca ctatgggcgt tattacaagg ctaaatccgg cggccagacc
     8461 cgagcccgtg gtctctaaca aaagaatggt cacggccagc aaccgaaagt tatccccaaa
     8521 ttccattata ccctgaccta tcagcaggcg gacaaagggt tttcgaaggg ctatatattg
     8581 taaggctcta cctcttctat tcatggaaca agacctcaat tatctatcta tacatgatag
     8641 tattatgtgt ttggtgatta tatgcggcaa aaagggtatc cgaagatacc ctaatcgttt
     8701 ctttctatta tactgggcat aggcctgtaa tagctggttc ccatgtcctt aatttctacg
     8761 gttccgtcgg ggttgctcac ggttaccccg gatttgactg tagcgccatc cataccatct
     8821 actaccctat gctcaacccc cggggagagt tccgggtttt tcttatacag tataggggcc
     8881 ttttggatat cggtaacatc atggtgccac tttatatcag gtggtttttc ttccccataa
     8941 aaagctatat agagcatatt ttctatgccc tcggcccata tgaggatggg aaatgaagtg
     9001 ttgttcttaa actgaaaatc cctgctccca tatgcaaccg tggcatcctg gccatagggt
     9061 acatagggta cgggcatgct gtggttatat ctttgtacta tctctaaatt gctaagtacc
     9121 gcaacattat agagggtgga cgaaatcttg cacacaccgc ccccgatggt ggtaataagt
     9181 tgcgtccctg catatgtggg accgctctta aaccccttgg attcatcata tggtcctatt
     9241 tcccggtttt gggagaatat cttgccaggc tctattatct tccccgataa aagccttgcc
     9301 cctaagtgta cgttatattc ttccccggga agagggtccg gtaataccgt acggtaggcc
     9361 gccatcagca ccttggtacc gtgttcattt ccggctttta gaaaatccgt gtccctttcc
     9421 cagggcagag tttcgacctg tccggatttt tcatccttaa cgacccttat ctctatatgc
     9481 ccgccgccgc ccttgcccgg ggcgccggtg ccgttatccc atagctgcgg cccttttgat
     9541 tttggtccct taatttcccc atcccctcta cggccggacc tgtggtctgc tgattttggt
     9601 ggatttggat ttaaggcaaa tagtaccgaa ataatcacca aggtgatcaa tattgttatc
     9661 agacttttcc tgctaaatat atgcccaccg cctttaaaat agtaattgta gcgataatcg
     9721 gggtatccca tgacaatcaa aggaaatccc cagcaccacg ggggccgata tggcctgtcc
     9781 cataggtact aaagccctgg gatggattcc ttcaccctgt cataatcctc catagtatcg
     9841 atgtctttaa gtatacccgg atcccttacc ggaacgggaa tgtaaccctt atccgatatg
     9901 aattccctta gggttgaata acgcccctcc gccaacaact cctccactag atgtccccta
     9961 atcaatatgg gatgcccatt ttcgccatta aaaaggggga caattatatc cccctctgcg
    10021 cccagcatct tcccataggt ctcccccgtt accagtggat aatccccggg aataaagaag
    10081 attctgtccc ccgagacatt cttaaagcct tccttcgctg agctaaacat tccctctctg
    10141 tagtgcgggt tgtataccat ccttactttt tgatactttg cgatgaccct tccgatattt
    10201 tcaaagttgt gtccacctac aactacgatt ttggaacaaa acctatacat cccggtaatg
    10261 cacctttcga tgagggttat gccgcccaga tcaagtagca tcttacagac ccctgtcctt
    10321 ttggaaagcc ccgctgctag gatcactcct tcaattaatt ccattatcta gatcacccat
    10381 ttgctaagta ttgatagtgc cagggtcacg accgcagcga ttggatttat tcgcgtgatc
    10441 atttcccctt cgaccttctc ccttgacctt ataaacctta tgtatagata tataagtcca
    10501 ctgttaatcg gactgtctac aagaaaaaac ttcatcaagg attggagtgt gggaacctgg
    10561 gactgtggca aggtcataaa ttggacggcg gtaaagacca tccgccaaga taggctgaca
    10621 aacaaggcct cgaggtaacc aaatccccta tccctttttt cggccaatat aaatccgcca
    10681 tagacggcca tgccctctag aactatagcc atagcgggat atattatcct tattgggtca
    10741 tgtccgggaa taaaaaagtc caggagcttt atacatgccg ccagcactgc catataaatt
    10801 ataggttccc tcttggccgt ttccttgcga agccggttca tatagtaaca ggccatagga
    10861 aagagaaaga agctggtgac tccacggtaa ccttgaagcc taactaaatg caatatatag
    10921 ccacaggtgc gggaataaag cctagggcat tttgtatcct gtcccccttg gatatccctc
    10981 cctggttgag gctatcctcc atctccaatg acctgaaggg aaagttacac aatccggtaa
    11041 agcctgcaag tatcctgtta tcctttttga gagccactac ggtgattagg ggataatcga
    11101 tcttatctat cttggtagtc tttctgtgga agaagggcag gcttgtattt gaagaatcca
    11161 tcataaactg tgtgatgaat tcaccctctt ccaatttagt ccttccatca aagatctgac
    11221 ttaatggggt ctcccttgtt ccgccgggcc ctgcgatgga caccctgcaa tcggcgatca
    11281 acagaggcag cgatgcttcc ctgtacatta ttgtccccga gatattcccc ccaagggtaa
    11341 tctttccctg cagggtatgg tccgcaatcc ttgcgacagt tattccaagc atgggaaaga
    11401 gattggattc ggataattta gtgagggttg tagccgctcc aatgacaagg tcggtttccg
    11461 catatcccag cacgttacac tccgggatcc cctttatgtc tatgacggca tcgacgtaaa
    11521 tgctgttggt ccttgccata cttataattt ccgtcccgcc cccgtagtac atagtttttt
    11581 tcccctgtcc ggcgatgccc ataaagaggt ctacggcctg ggagatgttg tctggtctat
    11641 aatattcaaa atcaaaggga atcatgacaa tgcccccgtc ttcattttcc aaataagctc
    11701 cggatcgatg ggcacctgat ccaattccaa ttccgctgcc cgggttaagg catttgccaa
    11761 ggccgccggc atcccaagta ttccatgttc tccaaatccc ctggctccat agggtccgtt
    11821 ttctgccggg gtctctataa actctaccag gtagtccggc tgctggccaa acctcatcac
    11881 cttataggtc cgaaggctgc tgttcagtat gcccccgtcg ccgccgtata taaaggcttc
    11941 ccggctcccg agacccggac cttgccggtg ggatctatct tcacccttac gtttctggtc
    12001 tccatggcga tatggtccga ttttggcatg aagtattcgc cttccaccac cacatcgctc
    12061 tcttcccacc cccttttcat atcccctttt cggatctggg tatgatccgc tatattggtg
    12121 ttggctacgg gatatacatc gtcgattgcc tttttgtact gacctaaatt ctcgtggatc
    12181 aggggtgcgc ccgcctctat ggcctgggca ggacctttaa ccacaggcag gggctcgtat
    12241 tctatattga tcgaatacgc tgccagttgc gcctcgtgtt ccgtatctgc gactactata
    12301 gccacgggct ctccgaaata cctgacctta tctacggcta tgggcggacg atcctctatt
    12361 accgacccag tctttacggg tagataatcc ccggtcacca cagcccttac ccccggcacc
    12421 ttccatgcct ttgtgacatc aatggatttt ataagaccat gagagcaagt gcttgttacc
    12481 atcttcacat gtaaaagccc gggttccctt atatcgtcgt tatacttagc ggcgccggtt
    12541 accttgtcaa ggccttcctt tcttggaacg gttgtcccca caattttcaa gtactgccac
    12601 ctccaaatga attgctcgct tatcacccca ttatcttcag cttcaaatgt aaaaattata
    12661 catcccactg cggaattttg gaggttttat cggtctactg aaaatttcca aaatatttta
    12721 cagtatagct tgcagtttta ttaagaataa tatataattg aaatgaattc atttttgaaa
    12781 agttttaaaa tgcattaccg aaagttagaa cccaaaatgt gcactttcat atgcattgaa
    12841 acaaaatgga gtcataaaat gcataaggag gtgtatattt tatgcaaggt acagtaaaat
    12901 ggtttaattc agaaaaaggc tttggtttta tcgtaagcga agaaggcgaa gatgtatttg
    12961 tacatttctc agccatccag accgaaggat ataaaacctt agaagaaggc cagagagtag
    13021 aatttgagat tgaacagagc ccgcgcggac ctcaggccgt aaacgtagtt ccctcttact
    13081 aaatcaggac acaaaaagca ctgctagacg cagtgctttt ttattttatc cgctaaatta
    13141 caggcccctt cagattgaaa taccctgatt tgtattcgct ttcctccttt tcaaaactat
    13201 atagaggacc agaacggcaa ggccaagggc cgacagggca aagatgatcc taaattcccc
    13261 cagcataata ttgtcgttga gatagggtat acctgcaata aaacccttaa tcctgcctcc
    13321 tatccatggg gcaatgagca gggttatccc tatagccgcg gaatagaatg catcataaaa
    13381 ggaccttccc ttctccggca taatatcgta tcggtagtta aatgaaccca ccataaaccc
    13441 ggagttggca aacgaaccga ttccatagac caagggaata aagacataca tgcttccctt
    13501 tgtaaccagg gcccatagcg ccatctgcat ggcatagaag ccgtaggaca agtacatgac
    13561 aaagacattt ccatacctgt cccctatttt cccccactga gaataggcaa acatctgtaa
    13621 aacagcgttc agcatggtca tggacgttat gaaggtgtag gatagctcta aataccgtat
    13681 catatagacc tgggtatagg acgccgacag atacagggct atatgaaaga gcacgtttat
    13741 tacggtgtag tccataaatt ccttgttctt tatggggatt ctgaacacat ccaggatttt
    13801 gatacccttc ccgataccct ccacttgggc atcttccact ttggagaaag cgtaggtctc
    13861 acccatcccg aacacaaagg ctgccgcaaa gagtatgata aaccccaaat acctgctgcg
    13921 cgttgtatcc aatatacccc ctgcgacgat ggggagcaaa accatgacca aaaccgcgta
    13981 aatctgtctg atagaaaaat accttcccct gagctttatg ggaatagcct tgacaaacca
    14041 gctattgacg gccagaccta aaaatgcgtt taggatatag accacaaaga gcaggatgaa
    14101 caataccaag acctgtgccg gcttgggcac caacagggga attaaaataa ttgaaattaa
    14161 aagaggcttt actatagagt tcaatgtcag aaccagtttt ctccgtttgg taaaccgttc
    14221 catcaatatc ccgaaaaata tgagggaaat gccgcttata ccgccgatca acggtatata
    14281 ccctaccagt tcatcggagg cccctaaaaa tatggcaaat cccgaaagaa attctccgcc
    14341 ggtcagaata gcgaaggcat tggcgaatat cacctgaata ataaagaaat cttggtttct
    14401 tctgtttgga ctgtttataa gctctatat
//
LOCUS       DASRNX010000239        19068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_529650,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000239 DASRNX010000000
VERSION     DASRNX010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 19068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19068
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529650"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..746
                     /locus_tag="VFD89_09575"
     CDS             18..746
                     /locus_tag="VFD89_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HZJ58466.1"
                     /translation="MVACTSGASKSSKTFTVGFDQDFPPMGFVGDDGEFTGFDLELAA
                     EVAKRLNMELKLQPIAWDAKDGELSSKSIDCIWNGFTINGREDKYTWTEPYLENNQVF
                     VVRSDSDIESLEDLADKAVVVQVDSSAEKALEGEPDLVASFGEYTAAANYNVALMDLE
                     AGAVDAVAMDEVVAIYQNEKRGEIFAILDEVLAPEEYGVGFLLGNEELRDKVQEALEA
                     MAADGTMAKISEKWFDEDITTIGK"
     gene            1014..1649
                     /locus_tag="VFD89_09580"
     CDS             1014..1649
                     /locus_tag="VFD89_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZJ58467.1"
                     /translation="MIYKLSEGMLESIKIFTLTLLFSLPLGLFVAFGRMSKNWILKNF
                     TKLYISIMRGTPLILQLMMIYFGPFYIFGINLPRGYRFFAVIIGFVINYAAYFAEIYR
                     GGIESIAIGQYEAAKVLGYSRAHTFIKIILPQVIKRILPSITNEVITLIKDTSLAFAI
                     SHSEMFTVAKAISAAQTTMTPFLVAGIFYYVFNFIVAFMMEWLEEKLGYYR"
     gene            1660..2424
                     /locus_tag="VFD89_09585"
     CDS             1660..2424
                     /locus_tag="VFD89_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZJ58468.1"
                     /translation="MNLLEIRNLKKNFDDLKVIRDISMDVGKGQVVGIIGPSGSGKST
                     LLRCATLLETIDSGDLIYMGENAATTNANGGADYAPKAELKRIQSYFGLVFQNFNLFP
                     HYSVIKNVTDALVTVQRKSKGEALEIGREFLNKMGLSDKEEAYPWQLSGGQQQRVSIA
                     RALALNPQVLFFDEPTSALDPELTGEILRVIRDLAAENMTMVIVTHEMAFARDVADHI
                     IFMDEGVIVEEGAPENIINNPQNERTQAFLNRVSIM"
     gene            2718..3404
                     /locus_tag="VFD89_09590"
     CDS             2718..3404
                     /locus_tag="VFD89_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZJ58469.1"
                     /translation="MSIMGNLYGFFGYYNHSRGKYKKALDMYERSEKLGIDRPNYLLS
                     YGVLMLREGNFAKAKELFSTVLTDLPAKEEHKTMAKMNISLAYWKLGDIDVAVEMLEE
                     VHRKHRSGKVYGTLGYLLIQKGDLERALEYNLEALDYDEDDPVILDNLGQIYYKMGQV
                     NKAKDFFERAEEEKDDQAVTLYYLGIIYSREGSTEKAREKLNKALTCNITPLSAITHG
                     DIEKALGDLD"
     gene            complement(3387..5888)
                     /locus_tag="VFD89_09595"
     CDS             complement(3387..5888)
                     /locus_tag="VFD89_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HZJ58470.1"
                     /translation="MEQKGPIPTRLDKRSNNRPNRLKRAMRIIIAIPLIGLAVLLTIA
                     AFVMGMTINSWKELDIGKLENIQESSFIYDYQDERITNIHGSENRIKVSLSDIPKHVQ
                     DAFIATEDIRFYTHPGFDIKRLASSLWHNIKARAYVQGGGTITQQVVRNAFLSQKKVM
                     SRKIQEIYLAYQLEKQYSKEQILQTYLNLIYFGKGTYGVETASNRYFGKSVKDITISE
                     GAMLAGIIKNPGRYSPLIDRANSLKRKDLVLDLMVKNGYLTDNEGKNAKEEPIELVET
                     PPIGYVHSYFMDMVLKEAADLLKVKEETLFTNGYKIYTTLDRDLQEFAEAIFHRDEFF
                     PKSPVSGESCQGAMVVLNSSTGEVRAIIGGRSDDKSIRKAFNRATQARRQPGSVIKPL
                     VVYAPAIENFGYTAATFIEDTPVTIDNYSPSNYGGKTRGWVTLREAIASSINIPAVKV
                     LHDIGIRNGISFAKGLGIPFADEDQNLSVALGGFHYGISPMELARAYMAFADKGKYKE
                     HTTIRRIEDPKGLVVYEFNPHKTQYVSEETTFIINNMLQSSIEWQGGTAARLKSLGIP
                     LAAKTGTVQLPDIQEFRGINGTKDTWIAVYNPDYVLAVWLGFDETNSQNYLPATAVGG
                     SFTADISGEIIRHIYANQGIKSFERPLNVLEVELDAKALKERHQALLASPLTPQDQII
                     LEYFTRGNVPRQETDYWVVPDPPKDFEITLTPRGLPTISFSTTQAFAVYDIYKSTGRT
                     GAAPVRRIEQGNQSRLQWTDKSVNPGETYSYYVVALHPEITMDEMPVQSLPTPVLTLT
                     VPPRDNTNPNDREQDKDMEQDIQTQEDDIISIQIP"
     gene            6324..8078
                     /locus_tag="VFD89_09600"
     CDS             6324..8078
                     /locus_tag="VFD89_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HZJ58471.1"
                     /translation="MSKKHRIVIAIVAALLLFATYGVWQNRRPTPKKISYNEFLHEVK
                     AKRVEKVFLNGGASIRGTFKGDIKFITDNPRKEGLKEELLREDIIVDEMEGREVINQI
                     LGYAVMAGLLIFSMVYITKKAPQTKGRTSKVSGMNIKPEESVKVKFSNIAGNEEALHS
                     VEELIDFINNPGKYSQYGARLPRGVILYGPPGTGKTLLAKAIAGEAGVPFYAVSGSDF
                     VQVYVGVGASRIRELFKMARQVGKCVIFIDEIDALGKKRDGGLEGGGNDERDQTLNAL
                     LAEMSGFNENEGIVVVAATNRLDTLDEALLRPGRFDRHIEIGLPDVKARLKILGLHSR
                     NKPIGDEVNLEKLAQQTVYFSGAQLENLMNEAAILAAKKGNGVIEHEDIEVAFYTVVA
                     GFEKKDRSSISEIDRKITAYHESGHALITKLVAPENRVSKVTIIPSTKGAGGFSMNIP
                     PDRMYSKKRDMENNIKIMLAGRCAEEIIFGKENITTGASNDLEKATKVLVDMVRRFGM
                     TDSSGLLNYDVLYENGIRDVQDNFLDEAKDILDNLYSGVRDLLMEHKPLLEAIALKLL
                     EEETLEEEQLDYLVQKAG"
     gene            8242..8862
                     /locus_tag="VFD89_09605"
     CDS             8242..8862
                     /locus_tag="VFD89_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZJ58472.1"
                     /translation="MAKETLDIGTRIPGIKAHSLRKQGYVPGIMYGRDRQTSPVMIES
                     KGLNTLLRGAGNNVFFEIALENFVGPVRLKEVQRDPVTRDIIHVDIQMINENQKVKAD
                     VPVRIHGSYESGKRGIVLQRQRDSIQVEGFPQHIPDQIDVVVRDLVQGGNIRVGDLEI
                     SEELSIVGNMEDVILSAVKSSRLEIENIVEQDGLQDHGETDAEAES"
     gene            9036..9257
                     /locus_tag="VFD89_09610"
     CDS             9036..9257
                     /locus_tag="VFD89_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58473.1"
                     /translation="MKYVYAILIVVDNNPIMQTYVSDKAMTGRQIMDEVILTGEERIK
                     YAHWEDENKILEDMNKNQSIRVEIQEVGV"
     gene            9425..9769
                     /gene="spoIIAA"
                     /locus_tag="VFD89_09615"
     CDS             9425..9769
                     /gene="spoIIAA"
                     /locus_tag="VFD89_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HZJ58474.1"
                     /translation="MQITNKRVSDILVVSLEGELDHHTSGEVRKYIDHLLDDPTIKNL
                     IIDIKKLNFMDSSGLGVLIGRYKIIKKRGGKLGVSNISAQIDRIFVVSGLYKIIKPYE
                     GLQQALKDIGGY"
     gene            9771..10223
                     /gene="spoIIAB"
                     /locus_tag="VFD89_09620"
     CDS             9771..10223
                     /gene="spoIIAB"
                     /locus_tag="VFD89_09620"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HZJ58475.1"
                     /translation="MDKINEMKLRFVSKSSNESFARVTIAAFASQMDPTLGEISDIKT
                     AVSEAVTNAIIHGYEDKMGYVEVLATIFSDRLVVEVSDRGRGIEDIEKARQPLYTSKP
                     ELERSGMGFTVMETFMDDVRVVSGINQGTKVIMIKYIGSLTDNIGGSK"
     gene            10220..10984
                     /gene="sigF"
                     /locus_tag="VFD89_09625"
     CDS             10220..10984
                     /gene="sigF"
                     /locus_tag="VFD89_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HZJ58476.1"
                     /translation="MSTGYAAWENNDNTLLSHSDTMVLIKKAQGGEQQAVDILVQKNV
                     ALVKSIIKRFLNRGHEYDDLFQIGVIGLIKAIRNYDPQYNVRFSTYAVPMIMGEIKRF
                     LRDDGLVKVSRSLKELANKSLAMKEQLKNVLHREPSIQEIAEAVGSDPEDIVHALEAS
                     RMPASIYDVVYEDDDNPILLIDRVSREDAQMSQVVDRVALKDVLSKLDERERYIIIMR
                     YFHDKTQSDIAGELGISQVQVSRIEKKVLLRMRELI"
     gene            11187..11366
                     /locus_tag="VFD89_09630"
     CDS             11187..11366
                     /locus_tag="VFD89_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58477.1"
                     /translation="MIKTKKRIYILIFIILGLALLGSAVIIWDVMGNYGKQQDKVYSG
                     AKLVYSIIDHPLKIG"
     gene            11370..11987
                     /locus_tag="VFD89_09635"
     CDS             11370..11987
                     /locus_tag="VFD89_09635"
                     /inference="COORDINATES: protein motif:HMM:NF023586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AA"
                     /protein_id="HZJ58478.1"
                     /translation="MENFIYLQLEDYFEAEVGQDVYLKDIAELSCDPEAGRRLAELVI
                     LEDNRVGTVNLGAIEVIERIRRIMPGVPVLSVGKPRIIIHIFEKKGINKDVVAGPIKA
                     AITGLLLFIGSGLVIMYFHEDVNMRQVHGAIYTAVTGQIDPNSYWISIPYSIGLGVGI
                     AFFFGILTNKRKPVKPDPLEIGISKYKKEIRSYLGTKKDRNQKNR"
     gene            11984..12400
                     /locus_tag="VFD89_09640"
     CDS             11984..12400
                     /locus_tag="VFD89_09640"
                     /inference="COORDINATES: protein motif:HMM:NF025155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AB"
                     /protein_id="HZJ58479.1"
                     /translation="MKALFLITIGLSGGAVVGAGVFAFFVALGVIERIIDISKTETYG
                     KAYRISILLGSLLSTFIYMFGFGIEANRGWLVVIGLFMGAFVGMIASALAEVLNVIPF
                     LSITMRIDRWVYFLIAAIILGKVLGSIVYWSLLIAQ"
     gene            12567..12719
                     /locus_tag="VFD89_09645"
     CDS             12567..12719
                     /locus_tag="VFD89_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58480.1"
                     /translation="MRKGYHRIPIVEHEGRNPEEKEDRHDLDKRLIDDREARNMDAGG
                     DLSSPH"
     gene            12852..13316
                     /gene="spoVAC"
                     /locus_tag="VFD89_09650"
     CDS             12852..13316
                     /gene="spoVAC"
                     /locus_tag="VFD89_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HZJ58481.1"
                     /translation="MDSVDVKQEKINKEYQDYANSLSPKSNIISNCIMAFLVGGLISI
                     IGQLISNVAKTYYNMNEEGAATVTSITLIFIAALLTGLGLYEKIGKKAGAGSIVPITG
                     FSNSIVAPAMEHKREGYVLGVGAKLFSIAGPVLVYGISTSVIVGLINYFFVK"
     gene            13329..14345
                     /gene="spoVAD"
                     /locus_tag="VFD89_09655"
     CDS             13329..14345
                     /gene="spoVAD"
                     /locus_tag="VFD89_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HZJ58482.1"
                     /translation="MSMHRMGGQTLLLTQNPPKIISRATIVGKKEGEGPLAEYFDKIL
                     EDDLWGEKSWEKAERRMYVEAAELALKKAQKEPQDINYLFGGDLLDQIISATFAARSL
                     GIPFYGLYGACSSMTQTISLAAPLLDGGYADNVLCVTSSHFCSAERQFRYPLEMGVQR
                     PPTAQWTVTGAGGVLLSVNGEGPCITHVTTGKVIDFGMKDANYMGAAMAPAAADTFRA
                     HFEDTGRGPDDYDLIVTGDLAKYGRELTIDLLKRDGYDIEDRFIDCGCEMFNESQDVH
                     AGASGCACSAVVLAGYLLKKMEDGDIKRILMASTGALHSIITSQQGDSIPGIAHAIAI
                     EKKG"
     gene            14349..14705
                     /gene="spoVAE"
                     /locus_tag="VFD89_09660"
     CDS             14349..14705
                     /gene="spoVAE"
                     /locus_tag="VFD89_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HZJ58483.1"
                     /translation="MDYIKAFITGGVICIIGQILIDKTNLTPARILVIFVTAGAILTA
                     LGIYGPIVEFGGAGATVPLPGFGYALAKGAIKGVKDSGVLGAFTGGVTAAAGGITAAI
                     VFGYLAAVLFDPKEKS"
     gene            complement(14810..17770)
                     /locus_tag="VFD89_09665"
     CDS             complement(14810..17770)
                     /locus_tag="VFD89_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZJ58484.1"
                     /translation="MDKIKKWLGSFLKPKPKSNRPTKQSAENNNKQDKRNHPIRGLFV
                     RKRRQPNFFLSVFVTTLRLFLILFILVGFVGFGAVVGIGKAYLDSTPELDVVQIQEQS
                     ETTFIYDKYGNLITEYYGYENRIWAAIDEIPETLRKAFIAIEDTRFDVHNGIDIKRIF
                     GSIIHNLSNESVQGASTLTQQLLKNTILTPEVSYKRKIQEMYLAIQLEKRYSKDQIME
                     AYLNTIWLGGSNYGVKAAAKDYFGKELNELSLRECAMLAGITQNPWQYDPRANTYIRD
                     NPEPTYKRTNLVIDRMYENTFISESEYQRARIDESEYYSKPVHIEENPTSNDYPMKYF
                     IEYVIKDVRDRIMVLEGWEGEEGRRKAEQYIYSGGLHIYTTVDPNIQQVVEESVYNYK
                     NLPKFQNSANKFSKQGVEQPQAAAVVIDQHTGELQAIVGGKQPPTGKRQYNRAFHPDA
                     KLPLGSSIKPLSVYGPFIEAGYPGGIIIENIPAAIDGWKGGERPYPRNYGDSRYDGPT
                     DVHRGIFKSLNVVAARIILERIGPQYSADKLKELGFDSKDVSEEPAALSLGTRGNNMI
                     EVAGAYAAIANKGIYQEPISFTRIVDKHGKEIINIRKDQVTRVVFKESTAFILTDWMQ
                     DVVDGGTATVRIKNGNGKRIKAAGKTGTNSDFKGVFFAGFTPYYTSTVWLGHDDFQPR
                     FVKNTTGGKFAAPLWETIMNSIHVDLEGKEFYDRVPDDVVRFTVCGVSGKRPNGELCG
                     EDISGHKLVTEWFPKNAIPGHDEICDMHVQIEACPYSGKPPTPYCPADHKTKASAILL
                     PKNSPYRKLSDKDLAKYLPGAVKDTKRLANLDYNNPNDRPFFCPLHTEEWYKGELERE
                     SMTGKASALIAQVKSNMANPKYIPKLTEKRLDKLHKAIAKLEGALKQGVIDKPEKPNK
                     DKGHIKELPIFDADLVEKEMENLEKISKEIFAAVEKELEKDKPKPDPVIPDPPEGGDE
                     EPPADNGGENNEGE"
     gene            18243..18980
                     /locus_tag="VFD89_09670"
     CDS             18243..18980
                     /locus_tag="VFD89_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZJ58485.1"
                     /translation="MAGHSKWANIKNKKGKTDAERGKIFTKIGRQIAVAVKEGGSDPE
                     SNARLRDAIASAKAANMPNDNINRSIKRASGELGNVTYEEVTYEGYGPSGVAIIVQAL
                     TDNRNRTAGEMRYIFDRSGGSLGATGCVSWMFDRKGLIVIEDNQEVDEDDLMLSVLEA
                     GGEDIELEDGIFEILTDPSVCTAVREGLEGDAYNIASSEVVMIPQNFVELVGDEYHGA
                     LSIFERLEDHDDVQNVYHNLRLQDEED"
BASE COUNT         5456 a         3647 c         4652 g         5313 t
ORIGIN      
        1 ccgtattggc gctgagcatg gtagcatgca catccggcgc atccaagagt tccaaaacct
       61 ttaccgtggg ctttgatcag gatttccctc caatgggttt tgtcggggat gatggggagt
      121 tcaccggatt cgatctggaa ttggcagctg aagtcgcgaa gcggctcaat atggagctta
      181 agctgcaacc gattgcttgg gatgcgaagg atggagagct atcatctaaa agtattgatt
      241 gcatatggaa cggttttacc atcaatggaa gggaagacaa atatacatgg accgaaccct
      301 atctggaaaa taatcaggtt ttcgtcgtta ggtcagattc agatatcgaa tccttagagg
      361 atttagcgga taaggctgtg gtggttcaag tagactccag cgcagagaaa gccttagaag
      421 gcgagccgga tttagtagca agttttgggg aatacacagc agccgccaac tacaatgttg
      481 cactcatgga tctggaggct ggagcagtgg atgccgtagc tatggatgaa gtcgtagcca
      541 tctatcaaaa tgaaaagagg ggagaaatat ttgctatact agatgaagtt ctggcccctg
      601 aagaatacgg agtagggttt cttttaggaa acgaggaact cagggacaag gttcaagagg
      661 cgttggaggc tatggcagct gacggcacca tggcaaagat ttcggaaaaa tggtttgatg
      721 aggatatcac aacaatcgga aaataggaca atttcaccct tcaatgattt tatgggttag
      781 atgatataat tcatgctata gtgagaggtc ttctgcatag catacccgga tgttttatgc
      841 caaatgattc cctttggatt catatggcat aaaacatttt ctataacttt acattacatt
      901 tagaaaaata cattgggtgg ggataaatat attccatatc atgaacaggt aatcaaaagc
      961 ccgttaatgg ctataggaaa gcaaaggtgg tatattaagt ggcatggtcc gtaatgatat
     1021 ataagctcag tgaaggcatg ttagagtcca ttaagatatt taccctaaca ctgttgtttt
     1081 cgcttccctt aggcctgttt gtagcctttg gacggatgtc aaagaattgg attttaaaaa
     1141 actttaccaa attatatatt tcgattatgc gggggacacc attaatcctc cagctgatga
     1201 tgatttattt tggcccgttc tatatctttg gtataaatct acctagggga tatcggtttt
     1261 tcgcagttat cataggcttt gttataaact atgccgcata ttttgcggaa atctatcgcg
     1321 gtgggatcga atcaatagct atcggtcaat acgaagccgc gaaggtactg ggatacagca
     1381 gggcacatac ctttattaaa ataattttac cccaggtaat aaaacggatt ttgccgtcca
     1441 ttaccaacga ggtcataacc ctcattaagg atacttcact ggcattcgct atttcccact
     1501 ctgagatgtt cacagtggca aaggcaatct ccgcggccca gacaaccatg acaccttttc
     1561 tggtagccgg aatattttat tatgtattca attttatagt ggcctttatg atggaatggc
     1621 tagaggaaaa attgggctat tatagatagg agggataaag tgaacctatt ggaaataagg
     1681 aatttgaaaa agaatttcga cgatctcaaa gttatcaggg atatatccat ggacgtgggt
     1741 aaagggcaag tcgtaggcat tatcggacct tcgggatctg gaaaatcgac cctcctaaga
     1801 tgtgcgacgc tgctggaaac aatagattct ggggatctta tatatatggg ggaaaatgcc
     1861 gccactacca acgcaaatgg aggggctgac tatgccccga aagcagagct gaaaaggatt
     1921 cagagttatt ttggattggt ctttcaaaac tttaatctat tcccccacta ctcggttatt
     1981 aaaaacgtaa cggatgcgct ggttaccgta caaaggaaat ccaaagggga agccttggaa
     2041 ataggcaggg aatttttaaa caagatgggg ttatccgaca aggaggaagc ctatccatgg
     2101 caactatccg gcgggcaaca gcaacgggta tccatagcca gggcattggc gcttaacccc
     2161 caagtactat tctttgacga acctacctct gctcttgacc cggaacttac gggcgaaata
     2221 ctgagggtaa taagggattt ggcagcggag aatatgacca tggtaatagt tacccacgag
     2281 atggccttcg ctagggatgt agcggatcat attatcttca tggatgaagg ggttattgtc
     2341 gaagagggag cgcccgaaaa cattataaat aatccccaaa atgaaaggac ccaggcattt
     2401 ttaaacaggg tctcaattat gtagcgtttc agattatatt ctatccatgg acattttcat
     2461 caataattca tataatatgg gttagatagc ccttggcttt gaatcaatgc cttcatctgt
     2521 tataacgagt cggtaagatg tctcccgctt ctataagcgg cgagttccca ttagtaaaac
     2581 aggcggtgga tataaatcac ccgcctcgtt gaaacctgtg tgttgcaatt gctaatgcaa
     2641 ttgcttccga tgttataacg agtcggcccg cattatatga aaaatagctt tgtaaaggca
     2701 agaaaggatg atgccaaatg tctataatgg gaaatctgta tggttttttt ggatattata
     2761 accacagtag gggcaaatat aagaaagcct tagatatgta cgaaagatct gaaaaattgg
     2821 ggatagacag accaaactat ctgctatcct atggggtctt aatgcttcgg gaaggaaact
     2881 ttgcgaaggc aaaagaattg ttcagtacag tcttaacgga cttacctgca aaagaagagc
     2941 ataaaaccat ggccaagatg aatattagtc tagcctattg gaaattaggg gacattgatg
     3001 tggccgtgga aatgcttgag gaagtacata ggaaacatcg cagcgggaag gtgtatggca
     3061 ccttgggtta tctgcttatt cagaaagggg atttggagag ggccctcgaa tacaatctag
     3121 aagctttgga ttacgatgaa gatgatccgg tcattttgga taaccttggc caaatttact
     3181 acaagatggg acaagtaaat aaagccaagg atttctttga aagggccgaa gaggaaaagg
     3241 atgatcaagc agtaacccta tattatcttg ggatcatcta ctcccgggaa gggagcacgg
     3301 aaaaggcgag ggaaaagctg aacaaggctc ttacttgtaa cattacgccg ctgagtgcca
     3361 taacccatgg agatatagag aaagccctag gggatttgga ttgatattat atcgtcttcc
     3421 tgtgtttgta tgtcttgctc catatcctta tcctgttctc tgtcattggg attagtattg
     3481 tcccttgggg ggaccgtgag ggtcagtaca ggtgtaggca ggctttgcac cggcatttca
     3541 tccatggtaa tttcggggtg taacgccact acgtagtagc tataggtctc acctggattg
     3601 actgatttgt ctgtccactg gagtcttgat tgattcccct gctctatgcg cctgacgggt
     3661 gcggcccccg ttctgccagt ggatttgtat atatcataca ctgcgaaggc ttgggttgtt
     3721 gaaaaggata tggttggcaa gccccttggt gtaagggtta tttcaaaatc cttcggtggg
     3781 tcaggaacaa cccaataatc ggtttcttgg cgggggacat tgccgcgagt gaaatactcc
     3841 aggattattt ggtcttgggg ggtaaggggg gatgccaaca aagcctgatg gcgttctttt
     3901 aatgccttag catccaattc cacttccaat acatttaggg gtctctcgaa actttttatt
     3961 ccttggttag cgtagatatg ccgtattatt tcccccgaaa tatcagccgt gaaggagccc
     4021 cccacagcag ttgcgggtaa atagttttga gagttggttt catcgaatcc caaccacacc
     4081 gctagtacgt aatcggggtt gtagaccgca atccaagtgt ccttagtacc gttaatcccc
     4141 ctaaactcct gtatgtcggg gagctgcacg gttccagttt ttgcagctag tgggatacct
     4201 agggatttca gcctagcggc tgttccccct tgccattcaa tggaggattg taacatattg
     4261 tttattataa aagtcgtctc ttcggagacg tattgtgttt tatgggggtt aaattcataa
     4321 acaaccagcc cctttggatc ttcgattctg cgaattgttg tgtgctcctt atacttcccc
     4381 ttatctgcaa acgccatata ggccctagcc aattccatag ggcttatgcc gtaatgaaag
     4441 ccgccaaggg cgacggatag attttggtct tcgtcggcga agggtatacc caatcccttg
     4501 gcaaaggata taccgttcct aataccgata tcatgaagca cctttacagc tggaatattg
     4561 attgagctag ctatggcctc ccgtagggtt acccaaccgc gggtcttacc cccataattg
     4621 gatggggaat agttgtcgat tgtaactggg gtgtcttcaa taaaggttgc ggcagtgtat
     4681 ccaaaattct cgatagcggg ggcgtataca accaagggct tgatgacgga gcccggttga
     4741 cgcctggcct gagttgccct attgaaagct ttgcgtatgg atttgtcatc tgaccttccc
     4801 ccaataatgg cccgcacttc tcctgtagag ctattgagca ccaccatagc accttggcag
     4861 ctctccccgc ttacggggct ttttgggaaa aattcatccc tatggaaaat tgcctcggca
     4921 aattcctgaa gatccctatc gagggtggta tatatcttat atccattggt aaatagggtt
     4981 tcttccttaa cttttaaaag atccgctgcc tctttcaata ccatatccat gaagtagcta
     5041 tggacataac ctatgggcgg cgtttccacc aattctatgg gctcttcctt ggcgttttta
     5101 ccttcgttat cggtgaggta gccatttttc accataagat ccagcaccag atcctttctc
     5161 tttagggagt tggcccgatc tattaggggc gaataacgtc ccgggttctt tataatgcct
     5221 gccagcattg ccccctccga tatcgttata tcctttacag actttccaaa atatctattg
     5281 gatgctgttt caacaccata ggttccctta ccaaaataga tcaggttcag ataggtttgt
     5341 agaatttgct ccttcgaata ctgcttctcc aattggtaag ccagatatat ttcctgtatt
     5401 tttctgctca ttaccttctt ttgcgataaa aaggcattac gaaccacctg ttgagttatt
     5461 gtacccccgc cttgcacata tgctctggct tttatgttat gccatagcga ggaagcgaga
     5521 cgttttatat cgaagcccgg atgtgtatag aaacggatat cctcggtagc gataaaggca
     5581 tcttgcacat gtttgggtat atccgacagg gacaccttaa ttcggttttc acttccatgg
     5641 atattggtaa tcctttcatc ttgataatcg tagataaaag acgattcttg gatattctcc
     5701 aacttcccta tatccaactc cttccaggaa tttatagtca tacccatgac aaaggccgca
     5761 attgtaagga ggacagccaa gcctataagc ggaatagcta taatgattct catggctctc
     5821 ttcaatctat ttggtctgtt attgctccgt ttgtcaaggc gtgttggtat cgggcccttc
     5881 tgttccatat gtattccccc taagatcatg taattgaaga tattagcact taaagctatt
     5941 attcccacaa tttatatgta taaaccataa cctgccaagt attttaagcc cagattatcc
     6001 ataggcatta ggttatgggt ataccctgtc ttaaagaccc agcctaaaca tccttggcac
     6061 actattccat ggacaataga ccgggggttt tgtataatag tatagtagtg aagtattatg
     6121 taaactaaaa ttgataaaaa taattaaaat tgttctgttg gtgggcattc ccttataccc
     6181 gataaaacaa tattgggtgt attgtggcaa tgcttttgta ggcgtttact attttgatat
     6241 tagagtaata gaaaatgttc tatgttttat ggatccagat gaaatccaat gacgaagggc
     6301 atgcaagtag ggggagcgaa atagtgagta aaaagcatag aatagtcatt gctatagttg
     6361 cggctttact tttatttgca acatatgggg tatggcagaa tagaaggcca acccctaaaa
     6421 agatatccta taacgaattt ttacatgaag taaaggctaa gcgtgttgaa aaggtattcc
     6481 tcaatggtgg ggcatccata cgtggaacct tcaaaggtga tattaaattt ataacagata
     6541 atcctaggaa agagggctta aaggaagaac tgttaagaga agatattata gtagacgaga
     6601 tggaggggcg ggaagttata aatcaaatac tcggctatgc tgtcatggcg ggattattga
     6661 tttttagcat ggtgtatatt acaaagaagg ctccacagac caaaggaagg acttccaagg
     6721 tctcgggaat gaacataaaa ccagaggaat ccgttaaagt aaaattttcc aatatagccg
     6781 gcaatgagga ggcgctccat agcgttgagg aattgatcga ttttattaac aatcccggga
     6841 agtactccca atatggggcc aggctcccaa ggggcgtaat tctgtacggc ccacctggta
     6901 ctggaaagac tctcctggct aaagccatag ccggagaagc aggagtcccg ttttatgctg
     6961 tatcgggatc agattttgtt caagtctatg taggggtagg agcctctaga atcagagaat
     7021 tgtttaagat ggccaggcag gtggggaaat gtgttatttt tattgatgaa attgatgctc
     7081 tgggcaaaaa gagagacggg ggcttagagg gcggtggaaa cgatgaaagg gaccaaacct
     7141 taaatgccct cttagcagag atgtcgggct ttaatgaaaa tgaaggaata gtcgtagtag
     7201 cagctaccaa tcgtttggat actttggatg aggcgcttct aaggcccggg aggtttgatc
     7261 gacatataga gataggttta cccgatgtga aggccagact aaagattcta ggccttcata
     7321 gtaggaataa acccatcggt gatgaagtga atttagaaaa gctagcccag caaaccgttt
     7381 actttagtgg cgcacagctg gaaaacctca tgaacgaggc tgcaattctg gctgcaaaaa
     7441 agggtaatgg cgtgatagag catgaggata tagaagtggc cttttatacc gtagtagccg
     7501 ggtttgaaaa aaaggacaga agctccatat ccgaaattga taggaaaatt acagcctatc
     7561 atgaatcagg gcacgcactt attacaaagt tggttgcacc ggaaaacagg gtatccaagg
     7621 ttaccataat acccagcaca aagggggccg ggggcttcag tatgaatatc cctccggatc
     7681 gcatgtattc taaaaaaagg gatatggaaa acaacataaa gatcatgctg gcaggacgat
     7741 gcgcggagga aatcatattc gggaaggaaa atataactac cggtgcctca aacgatttgg
     7801 aaaaagcgac taaggttctt gtagatatgg ttagaagatt cggtatgacg gatagttccg
     7861 ggttgttaaa ctatgacgtt ctctacgaaa acggtataag ggatgttcaa gataatttcc
     7921 tcgatgaggc taaggatatt ctagacaacc tttactcagg ggtcagggat ctgcttatgg
     7981 agcataagcc cctgctagag gccatagccc taaagcttct ggaagaggaa accctagagg
     8041 aagaacagct ggattattta gtgcagaagg cggggtagac ctttaagata tctcgtctgc
     8101 agagaataat attctatgtc aacccattat aatataagat agcacctgat cttatggcga
     8161 ataaaggggc tctggagatt attaattaaa acaagatctg ttggggataa taatgccata
     8221 atttgaaagc aggtgcttta tatggctaag gaaacattgg atattgggac aagaataccg
     8281 ggaataaagg ctcacagtct taggaaacag ggctatgttc ctggaataat gtacggtagg
     8341 gatcgccaaa cctcaccggt catgatagag agtaagggac ttaatacatt gcttagggga
     8401 gcgggtaaca acgtcttttt cgaaattgcc ctagaaaact ttgttggccc cgtcaggctg
     8461 aaggaagttc aaagggaccc ggtcaccaga gatataattc atgttgatat ccagatgatc
     8521 aatgaaaatc aaaaagttaa ggctgatgtg cccgtaagga tacatggtag ctatgaaagc
     8581 ggtaagaggg gtatagtctt gcagcgccaa agggattcaa ttcaggtgga agggtttccc
     8641 cagcatattc ccgaccaaat cgatgttgtt gttcgggatc tggtgcaggg cgggaatata
     8701 cgagtggggg atttggaaat atccgaggaa ctcagtatag taggaaatat ggaagatgta
     8761 attttgagcg ctgtaaagag cagtagatta gaaatagaaa atatagttga acaagatggt
     8821 ctacaagacc atggggaaac ggacgcggaa gctgaaagct aggcttccgt tatccctttg
     8881 attcgcccga ccaacttccc ttatacagct acagtgtggc ggcggggttt gcttattgaa
     8941 ttatggtaga aagtattgta aacttagata tggtttatta gtcgaatcta tgagaccagc
     9001 tattttacca tataaaaagg ggtttggatg cggttatgaa atatgtttat gcgatattga
     9061 tagtagtaga taacaatccc attatgcaaa cctatgtaag cgataaggct atgacgggga
     9121 gacagatcat ggatgaggtc atacttaccg gcgaagagag aataaaatat gcgcactggg
     9181 aagatgagaa caagatactg gaggatatga ataagaatca atccattagg gtagaaattc
     9241 aagaagttgg ggtttaaaga taaggatcgg aagtccctcg acacaaatcg agggacttct
     9301 gatttttttg taggctaatc aacaatagta gctattattc tatgaaggaa aacaaaggcc
     9361 tatgaagaat actaataaaa ggtatgggac aatcttacat tttatcggtc aggaggtcat
     9421 ggtattgcag ataacaaata aaagggtgag tgatatattg gtggtatccc tagaaggtga
     9481 gctagaccac catacatccg gtgaagtgag aaaatatata gatcatttac tggatgatcc
     9541 caccataaaa aacttaataa tcgatataaa aaaactgaat tttatggata gttccgggtt
     9601 aggggtgctt attggtagat ataaaatcat taaaaaaaga gggggaaaac tgggggtcag
     9661 caatataagc gcccagattg acaggatttt tgtggtgtcc gggttgtata aaataatcaa
     9721 gccatatgag gggcttcaac aagctttaaa ggatataggg gggtattgat atggataaga
     9781 taaacgaaat gaagttgagg tttgttagca aatccagcaa cgaatccttt gcccgtgtta
     9841 ctattgccgc ctttgcttca caaatggatc ctacccttgg ggaaataagc gatataaaga
     9901 ctgccgtttc ggaggcggtt accaatgcca tcatccatgg atatgaagat aaaatgggct
     9961 atgttgaagt gcttgcaacc atattttccg atagattggt tgtcgaggtc tccgataggg
    10021 gaaggggaat agaggacatc gaaaaagctc ggcaacctct gtacacctca aagcctgaat
    10081 tggagcgttc tgggatgggg tttactgtca tggagacatt tatggatgat gtaagggtcg
    10141 tatccggtat aaatcaggga acaaaggtga taatgattaa atacataggc tctttaaccg
    10201 ataatatagg gggatcgaaa tgagcaccgg gtatgcggca tgggaaaaca atgataatac
    10261 cctcttgtcc catagcgata ccatggtttt gataaaaaag gcacagggtg gagaacaaca
    10321 ggctgttgat attctggttc aaaaaaatgt tgctttagtg aagagtatta taaaaagatt
    10381 cttgaataga ggccatgaat acgatgatct atttcaaata ggggttatag ggcttattaa
    10441 agcaataaga aactatgatc cccagtacaa tgtgcggttt tctacctacg ctgttcccat
    10501 gattatgggt gagataaaga gatttctaag agatgatggg ctggtaaagg tgagtcgatc
    10561 cctaaaggag ttggcaaaca aatctttggc tatgaaggaa cagcttaaaa atgtcttgca
    10621 tagagaaccc agtatacaag agatagcaga ggctgtcggg agtgatcccg aggatattgt
    10681 acatgccctg gaagccagta ggatgccggc ttctatatac gatgtggtat atgaagacga
    10741 tgataatcct atcctcctga tagacagagt atccagggaa gatgcccaaa tgtcccaagt
    10801 ggtggatagg gttgctctca aggatgtatt gtcgaagctg gatgaacgtg aaagatatat
    10861 aataattatg cgttatttcc atgacaaaac ccaaagcgat attgccggcg aattgggtat
    10921 atcccaagtg caggtttcga ggatagaaaa gaaggttctc ctgagaatga gagaactcat
    10981 ttaacaccct gacagggtgt tttttaattg ggataaaaaa gaaaggtaat acacataata
    11041 accatatacc cagattaggg gatgttcaat gatggattca ccgacagggg aagcaacagt
    11101 aaataaatta gtgcttatgc ggatgttgac agaacggtta ttcttattca ttgtggaccg
    11161 caatttatgg aaggagatgt aaatgaatga tcaagacgaa aaaaagaatc tatattttga
    11221 tattcataat tctcggcctg gcattgctgg gaagtgcggt gataatttgg gatgtgatgg
    11281 gaaattacgg gaaacagcag gataaggtat attcaggcgc aaaattagtg tattcaatca
    11341 tagatcatcc tttgaaaata ggttagtaga tggaaaactt tatctacctg cagttagagg
    11401 attatttcga agcggaagtc ggacaggatg tatacctaaa ggacatagcc gaattgtcat
    11461 gtgatccgga ggccggtcgc aggctggctg aactggtgat tctcgaggat aatagagtcg
    11521 gtactgtcaa cttgggcgcc attgaagtta ttgaaaggat cagacggata atgccggggg
    11581 ttcccgtatt atccgtaggt aagcccagga tcatcataca tatctttgaa aaaaaaggga
    11641 tcaataaaga cgtcgttgcc ggccctataa aggccgcaat aaccggtctt ttgctcttta
    11701 tcggctcggg gcttgtcatc atgtattttc atgaggatgt aaatatgcgc caggtccacg
    11761 gggctatcta taccgcggtt acaggtcaaa tagatccaaa ttcgtactgg atcagcatcc
    11821 cctactccat agggctgggg gtggggatag catttttttt tgggatttta acgaacaaaa
    11881 gaaaaccggt caaacctgat cccctggaga ttggaataag caaatacaaa aaggaaattc
    11941 gctcgtacct gggcacaaaa aaagatagga atcaaaaaaa tcgatgaagg ctctctttct
    12001 tatcaccata ggattatccg gcggggccgt ggttggagcc ggggtattcg cgttctttgt
    12061 ggccctggga gtaatcgaaa ggataattga tataagtaaa actgagacat acgggaaggc
    12121 ttataggatt tctatccttt taggtagcct tttgtctacc tttatctata tgtttggatt
    12181 tggaatagag gcgaataggg gctggcttgt ggtaataggt ctatttatgg gtgcctttgt
    12241 gggtatgata gcctctgcct tagcggaggt actgaacgtg ataccctttc tgtctatcac
    12301 aatgaggatc gacaggtggg tatacttttt gattgcagcc attatcttag ggaaggtatt
    12361 aggctccata gtatattgga gcttacttat agcccagtga aacctccggg cccataacct
    12421 gatcttgcta actggagggt ccttggaaat tgcattgtta agccgtcgat tccacaatag
    12481 cggcggaatg aaaactaatt tatctataga taaattttat agccgaagtt gactacctat
    12541 ataaaacaca aggggagaag gacaatatga gaaaaggtta ccatcgcatt cccattgtag
    12601 aacacgaggg aagaaatccg gaagaaaagg aggataggca tgatctggat aaaaggctga
    12661 tagatgatag ggaggcccgg aatatggacg ccggagggga tttaagctcc ccccattaga
    12721 ttgactgtac cagatgatta aaacgggttt tgtaattttg ggtatttttg catcatacac
    12781 agaccatgat acccaagggt ctatgaataa aattgaattt aatgaaaatg atattctagg
    12841 gaggtgttcc tatggattct gtagatgtaa agcaagaaaa aataaataag gaataccagg
    12901 attatgccaa cagtctgagt ccaaaatcca atataataag caattgtata atggcgtttt
    12961 tagtgggggg cttgataagc ataatcggtc aactgataag taatgtggct aagacctatt
    13021 ataatatgaa tgaagagggc gctgcgacgg ttacatcgat taccttaata ttcatagctg
    13081 ctctattaac cggtttgggg ctgtacgaaa agatcggaaa aaaggcaggc gccggttcta
    13141 tagttcccat aaccggattt tcgaactcca tagttgcgcc ggctatggag cataaaaggg
    13201 aaggttatgt tttgggagta ggggcaaagc ttttttctat tgcagggccc gtcttggtat
    13261 atgggattag tacatcggtg atcgttgggt taataaacta cttttttgta aaatgaggga
    13321 tatcaattat gtctatgcat aggatgggcg gccaaacctt gctcctgacc caaaatccgc
    13381 ccaagataat ttcgcgggcg accattgtgg gcaaaaagga aggggaagga cccctggccg
    13441 agtattttga taaaatttta gaagatgacc tatggggtga aaagagttgg gaaaaggcag
    13501 aacgcagaat gtacgtggag gcggccgaac tcgctttgaa aaaggcgcaa aaagaacccc
    13561 aggacatcaa ttatttattt ggcggagatc ttttggatca aataatatct gctacctttg
    13621 ccgcaaggag tctgggcata cctttctatg ggttatatgg ggcctgttct tctatgacac
    13681 agactatatc gcttgccgcg cctcttttag acgggggata tgcggataat gtcctctgcg
    13741 ttacctccag ccacttttgt tccgcagaga gacaatttcg gtatcctttg gaaatgggag
    13801 tgcaaaggcc cccgactgcc cagtggactg tgactggcgc aggtggggtt ttgctttccg
    13861 tgaatggtga gggaccgtgt ataacccatg tgactaccgg taaggtaata gacttcggta
    13921 tgaaggatgc caattatatg ggtgcagcta tggccccggc ggccgccgat acctttaggg
    13981 cacatttcga ggatacaggt agaggcccgg atgattatga tttgattgta acaggtgacc
    14041 tggccaagta tgggcgggaa ttgaccatag accttttaaa gagggatgga tatgatattg
    14101 aggatcgctt tatagattgc ggttgcgaaa tgtttaatga aagtcaggat gtccatgccg
    14161 gggcaagcgg ttgtgcctgc tcagccgtgg ttctggccgg atacttgtta aagaaaatgg
    14221 aggacggcga cattaaacgt atccttatgg cttctaccgg agcactgcac agtattatta
    14281 catcacagca aggggattct atacccggta ttgcacatgc catagcaata gaaaagaagg
    14341 gatagactat ggattatatt aaggcgttta ttacaggggg agtaatttgc attatcggcc
    14401 agatcctgat agataaaacc aatttgacac ctgccaggat attagtaatt ttcgtaacag
    14461 caggtgccat tctaaccgcc cttggcatct atgggccgat agtggaattc ggcggtgccg
    14521 gtgccaccgt tccgttaccc ggttttggct acgctctggc caaaggggca ataaagggcg
    14581 taaaggattc gggagtcctg ggtgccttta ccggcggtgt taccgccgca gcaggaggaa
    14641 ttaccgcggc tatagttttt gggtacctgg ctgcagtttt attcgatccg aaagagaaga
    14701 gttgaaaaca aaaagggatg gctatccatc cctttagaag catattattc atcgcttgct
    14761 acttaaatat accattacgc tacctgatga acctgcacgg ggaaatattc tattcccctt
    14821 cattattttc gccgccgtta tcggcagggg gttcttcatc gcccccctca ggtggatccg
    14881 gaataacagg gtccggtttt ggcttatcct tttcaagttc cttttctaca gcggcaaata
    14941 tttccttgct gatcttctcg agattctcca tctctttctc caccagatcc gcatcaaata
    15001 taggtaattc cttaatgtgg cccttatcct tattgggctt ttcgggttta tcaatgacac
    15061 cctgtttcag cgctccttcg agtttcgcaa tcgccttgtg gagtttatcg aggcgctttt
    15121 cagtgagctt tggtatatac ttaggatttg ccatattact ctttacctgg gcgattagcg
    15181 ctgaagcctt accggtcatg gattcacgct ctaactcccc cttataccat tcttcggtat
    15241 gcaagggaca gaagaatgga cgatcattgg ggttattata atccaagttc gccagccttt
    15301 tggtatcctt tacggcaccg ggcaaatatt tggccaagtc cttatccgaa agcttgcggt
    15361 aaggagaatt tttgggtagg aggatggccg acgctttggt tttgtgatcg gcagggcaat
    15421 atggtgtagg aggtttcccc gaataggggc atgcctctat ctggacatgc atatcgcaga
    15481 tttcatcatg gcccggaata gcattttttg gaaaccactc ggtaacgagt ttatgtccgc
    15541 taatatcttc gccgcagagt tcaccgttgg gtcttttgcc tgataccccg caaaccgtga
    15601 atcttacaac atcatccggc accctgtcat aaaattcctt accctccaga tcgacatgga
    15661 tactgttcat aatagtctcc cagagggggg cggcaaattt accgccggtt gtgtttttaa
    15721 caaaccttgg ctggaagtca tcgtgtccca gccatacagt ggaggtatag tagggtgtaa
    15781 aaccggcaaa aaagactccc ttaaaatcgc tgttggtccc ggtcttgccg gcggccttta
    15841 ttcttttccc gttaccattt tttattcgaa ccgttgcagt cccgccatcg acaacatctt
    15901 gcatccaatc cgtaagtata aaagcggtac tctctttaaa aaccacccgt gtgacttgat
    15961 cctttcggat atttattatt tccttgccat gcttatcaac gatcctggtg aatgaaatag
    16021 gctcttgata gatgccctta ttggcaatgg cagcatatgc ccccgcgacc tcgatcatat
    16081 tgttcccgcg ggtgcccagt gacagagctg cgggctcttc ggaaacatcc ttggaatcaa
    16141 agcccaattc cttgagctta tctgcagaat attggggtcc tatcctttca agaataattc
    16201 tagcggctac tacgtttagg gatttaaaga tacccctgtg aacatccgta gggccgtcat
    16261 atctactgtc cccataattc ctaggatagg ggcgttcgcc gcctttccat ccatctatgg
    16321 ctgccggtat gttctcaatt attatcccgc ccggatatcc cgcttctata aaaggaccat
    16381 acacggaaag gggctttata ctggaaccca ggggcagctt cgcatcaggg tgaaaggccc
    16441 tgttgtactg acgttttccc gtagggggct gtttcccgcc gacgattgcc tgaagttcgc
    16501 cggtatgctg gtctataact actgccgcag cctggggctg ttcaacccct tgtttactga
    16561 atttattggc ggagttctgg aattttggta agttcttata attatagacc gattcctcaa
    16621 ccacttgttg gatattaggg tctactgtgg tatatatgtg caggcccccg gaataaatat
    16681 attgttcagc cttacgcctg ccttcttccc cctcccagcc ttccaggacc ataattctgt
    16741 ccctgacatc ctttatgaca tactctataa aatatttcat cggataatca ttgctggtcg
    16801 gattttcttc aatatggaca ggtttagaat aatattcaga ctcatctatt cgtgcccttt
    16861 ggtactcgga ttcggagata aaggtattct cgtacatcct gtctatcaca aggttggtcc
    16921 ttttataggt aggttcgggg ttgtccctga tgtaagtatt tgctctcgga tcatattgcc
    16981 aaggattctg ggttattccg gctaacattg cgcattcgcg cagggagagt tcgttcaatt
    17041 ccttaccgaa ataatccttt gctgccgcct taactccgta gttagaacca cccagccaga
    17101 tagtatttag gtaagcctcc attatttgat ccttgctgta cctcttctct aattgtatcg
    17161 caaggtacat ctcctggatt tttcttttgt agctcacctc cggggtcaat atagtattct
    17221 taagtaattg ttgggtaagg gtgctggccc cctgtacact ttcgttgctg aggttatgga
    17281 ttatggatcc aaagattcgc tttatatcta ttccgttatg gacatcaaat cgggtgtcct
    17341 ctatcgcgat gaaggccttt cttaaggttt cggggatttc atcaatggca gcccaaattc
    17401 gattctcata accataatac tccgtaatga ggtttccgta cttatcataa ataaaggtag
    17461 tctcactttg ctcttggatt tgtaccacat ccagctccgg cgtagaatct aagtatgcct
    17521 taccaatacc caccacagct ccaaaaccga caaatcccac taggatgaag agaattaaaa
    17581 agagtctcag ggtcgtaaca aaaactgata ggaaaaagtt gggttgcctt cttttcctta
    17641 caaaaagccc ccttatggga tggtttcttt tatcttgttt attattattt tctgcagatt
    17701 gctttgttgg acggttagat tttggcttgg gctttaaaaa gctgcccaac cactttttta
    17761 ttttatccat gatattcctc caattctcat atgtcagatt ccatttacat atgagagaga
    17821 ttctactagc attataacat acggagtcaa tacggtgaac aatcttttac catatttaat
    17881 tttgatatac aaggcagacc aaagcgagat cgtgtataat ttaatgcatc ctctgcgaaa
    17941 cactatggcc ttttagtaat ttgaatctct tctgggacga attgagacgg tcatttagcc
    18001 agattgttgc ggtgcgtcga ttatgggaaa cttccttgaa acagcgccgt gattactgta
    18061 taatggagcc ataaaatcgt gggtatacga acatggttat gatactttac acctatatat
    18121 cttgcactat tagaggacaa tagttgattt gatgttcaga aagttaaaca atttttttat
    18181 aggaaactca ttatacaaca tatttatgga acccattaat ggcagattag gagggtattt
    18241 atatggcagg gcattcaaaa tgggctaata ttaaaaataa aaaaggtaaa acggacgctg
    18301 aaaggggtaa gatttttacc aagataggca ggcagatagc tgtagcggta aaagaagggg
    18361 gaagcgatcc cgagagtaac gctaggttaa gggatgcgat tgcatccgca aaagcggcaa
    18421 atatgccgaa tgataatatc aacagaagca taaaaagagc ctcgggagag ttgggtaatg
    18481 taacctatga agaggttaca tatgaaggat atggtcccag cggtgtggca attatcgtgc
    18541 aggcactgac agataaccgg aataggaccg ccggtgagat gcgatatatc tttgatcggt
    18601 ccggggggag ccttggagca acaggctgcg tatcctggat gtttgatagg aagggactta
    18661 tagtcataga agataaccaa gaggtggacg aagacgattt gatgctcagt gtgctcgaag
    18721 ccggtgggga ggatattgag ctggaggacg gaatatttga gattttaacg gatccttccg
    18781 tctgtaccgc cgttagggaa ggactggagg gggatgccta taacatagcg tcttcggaag
    18841 tggttatgat cccacaaaat tttgttgaac tggttggaga tgaatatcat ggggctctgt
    18901 cgatatttga aagattggaa gatcacgacg atgttcaaaa tgtttaccat aacctgagat
    18961 tgcaggatga agaggattaa taagggtaga gcagatataa tcaatacatt tttcccatat
    19021 ataattgtat tccgggatct tcggtgcggt tgggaacagg tataatat
//
LOCUS       DASRNX010000240         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_472956,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000240 DASRNX010000000
VERSION     DASRNX010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472956"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..678)
                     /locus_tag="VFD89_09675"
     CDS             complement(31..678)
                     /locus_tag="VFD89_09675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZJ58486.1"
                     /translation="MLVPSSGDNAAVFAFRLLGATVTSTDISERQLENARRIAVAHDW
                     DIRFVCDDSISLTKIEDGVFDLVYTSNGVHIWIHDLLGMYRCFHRVSKKDGRYIMYEV
                     HPVIRPFGDDDVPIITVCKPYENIDINDGVQKFAWRIQDIFNAAFDAGFNITHMEEFH
                     PDRNDHDLWFYKNLAQAKADNYMKFDWEQNPWAVLPQWIGFSAKKVGSNQNRIMR"
     gene            complement(1095..2333)
                     /locus_tag="VFD89_09680"
     CDS             complement(1095..2333)
                     /locus_tag="VFD89_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58487.1"
                     /translation="MNTISKKDREILRELAKHLLGLANSKENEEVLNKWKLIERGEIG
                     EPTVRFLVSNFTNETITSRLKCEGENARDLEFGLLISSAGRELFNDDTPLTGEFLIHQ
                     NLWVTPFNTPAKRISAEGENAKGYHIEPVINDLEEDFDRLTVGDYGSNIQETNEKIEF
                     ARDVFGDILLVKGVMGSLSGPITSPLVHLMGMQNYYLSMYDYPDLLHKVMNNACKIYE
                     QFYDYLEDQGLLLPTAGVSPVAQESFAFNNELPKDRATKTTDCWGFLESQETTAVSSD
                     TFAEFVFPYQDRLAKRFGLLSYGCCERVDDIWEHISGWENLRKLSVSPFNDERRVGEY
                     LRGSNIVYYSKPRAEHVTIPGPLDEDAIIDCFKGIAEAASGCMLEVAQREVGTIFGDY
                     NRGRRYVELAKQTIDKYWKH"
     gene            2523..>2822
                     /locus_tag="VFD89_09685"
     CDS             2523..>2822
                     /locus_tag="VFD89_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58488.1"
                     /translation="MDLTVLISLKDTVSSLAEELGISRTAIYTWRRGKANDSGTPKPT
                     KRWSPKDKFHIVLETSTLTEQELAAYCRRKGLFVEDVKAWRDQCIKANASNSKDPF"
BASE COUNT          775 a          679 c          532 g          836 t
ORIGIN      
        1 attaatatat tccgcagcat tgccaaatct tcagcgcata atccgattct gattacttcc
       61 aaccttctta gccgagaagc ctatccactg aggcaacaca gcccagggat tctgttccca
      121 atcgaatttc atataattat ctgcttttgc ctgtgccagg tttttgtaga accataaatc
      181 atgatcattc ctatcgggat ggaattcctc catgtgcgtg atattgaatc ctgcgtcaaa
      241 ggcggcgttg aaaatatcct ggatacgcca ggcaaatttt tgcacaccat cgtttatatc
      301 aatattctca tatggcttgc agactgttat aatcggtaca tcatcatccc caaaaggacg
      361 gattacaggg tgaacctcgt acataatgta ccgtccgtcc tttttagaca cacgatgaaa
      421 gcatctatac atgcccaata gatcatgtat ccatatatgg actccgttgg atgtatacac
      481 gagatcaaac acgccgtctt cgattttcgt aagtgatatg ctgtcgtcgc agacaaaccg
      541 aatatcccag tcatgggcta cagcaatccg tctagcattt tctagctgtc gttctgatat
      601 atccgttgat gtcacggtag cccccagcaa acggaacgcg aatacggcag cattatcacc
      661 gctagatggc acaagcacat ttttacccct aagatttggg tatgagcttc gtatcatatc
      721 ccagactagg gcaggaaatg cccaggtcgg atcttcgatg atacacccga cgatcccttc
      781 ggaagcattt tcagcaaaat gtcatctgaa agagtgtccc attcctcttt tgccgtatga
      841 attattttat tctctcccat ttaatgctcc ccccaaaata aaatctgatt attccaagct
      901 cacttcctca ttacttatta tttgcagttg tatcatatat tccgtggtaa cccgtgcatc
      961 caagaccaag gtatgcaaca cgatgacttg aatgatgttc tatagcaaag ttttcaatca
     1021 actcacctat aatgcatggg aaattcggca tattcatatg ctaataaaaa tacaaaaacg
     1081 gtattataac acccctaatg cttccagtat ttatctatgg tttgctttgc tagttcaaca
     1141 tatctacgcc cgcgattata gtccccaaat atcgtgccga cctcccgctg tgcaacctct
     1201 agcatacaac ccgacgccgc ttcagcaatc cctttgaagc aatctattat agcatcttca
     1261 tctagtggcc ccggaattgt gacatgttca gcccgtggtt tactataata gactatatta
     1321 ctgccacgca ggtattcgcc cacccgtctt tcgtcgttga atggcgatac agatagcttg
     1381 cgaaggtttt cccacccact tatatgctcc caaatatcgt ctactctttc acaacagcca
     1441 taggacaaca acccaaaccg ctttgccagt ctgtcctgat atgggaacac aaattcggca
     1501 aaggtatccg aagatacagc cgttgtttcc tgtgactcta gaaatcccca gcaatcggtg
     1561 gttttagttg ctctatcttt aggaagctca ttattaaagg cgaaactttc ttgcgctacc
     1621 gggcttaccc ccgcagtggg taacaaaagc ccctggtctt cgaggtagtc ataaaattgt
     1681 tcatatattt tgcaggcatt attcattacc ttatgtagca aatcgggata atcgtacata
     1741 gacaaatagt agttttgcat ccccattagg tggacaagag ggctggttat tgggccgctc
     1801 agacttccca tgacgccttt aaccaggagg atatcaccga aaacatccct tgcaaactca
     1861 atcttttcat ttgtttcctg tatatttgaa ccatagtccc ccacagtcag cctgtcaaaa
     1921 tcctcttcaa ggtcgtttat aacaggctct atatgatagc ccttggcgtt ttccccttcg
     1981 gcggatatcc gttttgcggg ggtattaaat ggggtcaccc aaaggttttg atggatcaaa
     2041 aattcccccg ttaatggtgt atcatcgttg aatagttccc tgcccgctga tgatataagg
     2101 agaccaaatt ccaaatccct tgcattttcc ccttcgcact ttaaacggga tgttatcgtt
     2161 tcattggtga agtttgaaac aagaaatctt acggtgggct ccccgatttc gccacgctct
     2221 attaatttcc atttattaag tacctcttca ttttcctttg agttggcaag cccaagaaga
     2281 tgttttgcaa gttcccttaa tatttctcgg tcctttttgg atatcgtatt catcaacgtc
     2341 cccccttgtt atttatttga ttataacata ggttatatca catattatag gaataattca
     2401 gcaatatatc tcagttggta gactatgcat tactaaaatg ggttcttgta ttacattgga
     2461 gttaaggcac tggtatgtga ttttataaaa cattttactg ccgggataac ttacacacta
     2521 ttttggactt gactgtgttg atatccctaa aggatacggt atcaagtctg gcagaagagc
     2581 tagggatctc taggactgcc atatacacgt ggcgtagggg caaggccaac gactcaggta
     2641 ccccaaagcc caccaagaga tggagtccta aagataaatt tcatatagta ttagaaacct
     2701 ctacccttac cgaacaagag ctagccgcct attgccgcag aaagggatta ttcgtagagg
     2761 atgtcaaagc atggcgcgac caatgtataa aagcgaacgc atcaaattcc aaggaccctt
     2821 tc
//
LOCUS       DASRNX010000241         4461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_688960,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000241 DASRNX010000000
VERSION     DASRNX010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688960"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VFD89_09690"
     CDS             <1..928
                     /locus_tag="VFD89_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010283689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZJ58489.1"
                     /translation="FLVNTTAGVISLVTSVLMIAAALFITRWRYNQIGKLSGYLRRIA
                     GGEYSLDVRDNTEGELSILKSEIYKVTVTLSEQAELLQKDKLFLVDSISDISHQLKTP
                     LTSMSVMTDLLSDKNLPPDKRIEFTQNIRIQLERLEWLVSSLLKLSRIDAGTINFKKD
                     KVNVEELIERSIRHLVIPMEIKEQTLKIEGGSDVYFIGDFNWISEALTNVVKNCMEHT
                     PKGGEIGISFTGTSLYTMIAIKDDGPGISKKDLPHIFKRFYKGDNAHEDSIGIGLAMS
                     KSILESQGATIEVASDKGKGTRFTIKFYKGIL"
     gene            1089..1772
                     /locus_tag="VFD89_09695"
     CDS             1089..1772
                     /locus_tag="VFD89_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006211152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58490.1"
                     /translation="MEILRVENLSKIYGKGETAVKALDGVSLTVDRGQFIAIIGPSGS
                     GKSTLMHMIGGVDTPTEGKVFIENTDIYSLNETNLAIFRRRQIGLIYQFYNLIPVLDV
                     KENITLPLLLDGRKVHRGQLNEIVNTLGLMDRLDHLPNQLSGGQQQRVSIGRALINKP
                     ALLLADEPTGNLDSKNSMELIELLKLSNKKYNQTLIVITHDEGIALQADRIISIEDGR
                     IAKDEVIRQ"
     gene            1769..4363
                     /locus_tag="VFD89_09700"
     CDS             1769..4363
                     /locus_tag="VFD89_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZJ58491.1"
                     /translation="MNIINKLTFRQLKLNKRRTLVTIIGVIISVAMVMAVATLTVSFI
                     DLLRRQTIIQGGEWHVLYKGINADQLGAIKGDRLTKDVAITRDRGYAVLKGSKNDYKP
                     YLFIKEYDSQGFKQLPVELAEGRLPEANDEVVISEHIATNGKVEYEIGDVLELDIGNR
                     LIEGETGESYELGQNNPFQKIHGDEKRESLRIKESRSYKIVGVIKRPTWEPISAPGYT
                     IISYLDESLMGPSDLFNASVVFNKVNQSVYGHVEGFAGANDIGPESVTFNNELLRYSG
                     AISDDNTRKTLYSLSAIIMIIIMVGSISLIYNAFAISVSERSRYLGMLSGIGATRKQK
                     RNGVLFEGLVIGLVSIPTGIICGIAGMYVTFTFINTLIQGVFGIAERLRVIVTPLSIL
                     VASLVSAITILISAYIPAKRASKVSAIDAIRQSMDVRLTRKAVKTSRTVRALFGIEAE
                     IGLKNLKRNKRRYRATVFSLIVSIILFLTVSFFTNDLRKAVRMTQARINYDIYMNANV
                     DIDDGSIESIKSLPDIYEYSRVRKLYTYSWIEEEFVPEGLRDGGTEFRDGRYLYGIEV
                     QALDDDSLSRFAKIAGADYEKLIDPNDLSAIVIDTITYPDEGMGKFVETKAIKARVGH
                     KLDFINIDWEREVETDINSVKIIALTGESPMGVDPSPLGVINIVISERLMDSFVGSGE
                     NDPGFWDNLYLRSNNPIKTQEEIEKIQAVGLYIHNVYKQRQEEEQLILLMSVFIYGFI
                     TLITAISIANIFNTISTSIVLRKREFAMLKSVGMTPKSFNRMINYESIFYGIKSLLYG
                     LPISVAVMFLIHCSMEDSFEFGFSLPWRDVVIVIVAIFVIVGSTMLYSGGKIKKENIM
                     DGLKQEII"
BASE COUNT         1391 a          862 c         1063 g         1145 t
ORIGIN      
        1 cttcttagta aataccacgg caggggttat atcactggtt acgtcagtcc taatgatcgc
       61 ggctgctttg ttcatcacaa gatggcgata caaccaaata ggtaagctgt cgggatatct
      121 gagacgtatt gcaggcggtg aatattccct cgatgtaagg gataacactg aaggggagct
      181 tagcatacta aaaagcgaaa tatacaaggt taccgtaacc ctgtcagagc aggcagaact
      241 tttacaaaag gacaaactct ttctggttga ttccatttca gatatctcac atcagcttaa
      301 gaccccgcta acctccatgt ccgttatgac agatttattg agcgataaaa atttaccgcc
      361 ggacaagcgc atagagttta cgcaaaatat acgcatccaa ctggaaaggc tggaatggct
      421 tgtttcctcc cttttaaagc tgtccaggat cgatgccgga acgataaatt ttaaaaaaga
      481 taaggtaaat gtagaagagc ttattgaaag atccataagg caccttgtta tacccatgga
      541 gatcaaggaa caaaccttaa agattgaagg cggcagtgat gtctatttta taggcgattt
      601 caattggata tcagaggccc ttacaaatgt tgtgaagaat tgtatggagc ataccccaaa
      661 aggcggggaa ataggaataa gctttacggg gacttccctg tataccatga ttgccataaa
      721 agacgacgga cccggtatca gtaaaaaaga tttgccccat atcttcaaac gcttttacaa
      781 gggggacaat gcccatgaag acagtattgg gataggtctg gccatgtcaa aaagcattct
      841 tgaaagtcaa ggagcaacta tagaggtggc aagcgataaa ggcaagggta cccgatttac
      901 catcaagttc tataagggga tattatgaca aggggtaaaa aaatgggggt attgcgataa
      961 acggccatta taacggggaa tgatgtgaca aggggtattg tgacaaaact gtcacattaa
     1021 tagtcaccta aaagtcatac taggcaggta tactcagcct atagagaaaa aagaggtgaa
     1081 gtataatcat ggaaatacta agagtggaaa acctgtccaa gatatatgga aagggtgaaa
     1141 ccgccgtcaa ggccctggat ggggtatccc taaccgtcga tagaggacaa tttatcgcta
     1201 taattggacc gtcgggttcc ggaaagtcaa cccttatgca tatgataggg ggtgttgata
     1261 cgccaacgga gggtaaggtc ttcattgaaa acaccgatat ttactcctta aacgagacga
     1321 atctggccat cttcagaaga aggcaaatag ggcttatcta ccagttctat aaccttatcc
     1381 cggttttgga cgtaaaggag aacattaccc tacccctttt attggacgga cgcaaggtac
     1441 ataggggcca gctaaatgag atagtaaata ccctgggcct tatggatagg ctggaccatc
     1501 tccctaatca gctttccggc gggcagcagc aaagggtatc catcgggagg gcccttatca
     1561 ataagccggc acttttatta gcagacgagc ctacggggaa cctggacagt aaaaacagta
     1621 tggaactgat agaactatta aaactatcca acaagaaata taatcaaacc ttaatagtaa
     1681 taacccatga tgaggggata gccctacagg ctgatcggat catctccata gaggacggca
     1741 ggattgccaa ggatgaggtg attcgccaat gaacattatt aacaaactca ctttcaggca
     1801 attaaagcta aataagagac gaaccctagt aaccataata ggtgtaatta tttccgtagc
     1861 aatggttatg gcggtggcaa ccctcaccgt ttcctttatt gatttattgc gaaggcagac
     1921 tattatacag ggtggggaat ggcatgttct ctataagggt ataaatgcgg atcaactcgg
     1981 ggctattaaa ggggacagac ttacaaaaga tgtcgccatt accagggacc gtggctacgc
     2041 cgttttaaaa ggcagtaaga acgattataa accctatcta ttcattaaag agtacgactc
     2101 ccaagggttt aaacagttac ccgtggaact ggccgaaggc cgattaccgg aggccaacga
     2161 tgaagtggtg atatcggagc atatcgctac aaatggcaag gtagagtacg aaatcgggga
     2221 tgtcttggag cttgatatag gtaatcgctt gatcgagggt gaaaccggtg aatcttacga
     2281 gctggggcaa aataatccat tccaaaaaat ccatggggat gagaagcgag aatccctaag
     2341 gataaaagaa agcaggagtt ataaaatagt gggcgtcata aagcggccta cctgggagcc
     2401 gatctcggca ccggggtata cgattatttc ctatttagat gagagcctga tgggccctag
     2461 cgatttattc aacgcgtctg tagtatttaa taaagtcaat caatccgtgt atgggcatgt
     2521 ggaaggattt gccggggcca atgatattgg gccggaatcg gtcaccttta acaatgaact
     2581 gttaaggtat tcgggggcca taagtgatga caatacccgc aaaacccttt attccttgtc
     2641 tgctataatc atgattatca tcatggtagg gtccatttcg ctgatctaca atgcttttgc
     2701 tatttccgtt tcggaaagat cccgttatct cgggatgcta tcggggatag gcgcaacaag
     2761 gaagcaaaaa cggaacggcg tactctttga aggtttagtt atcggacttg tcagtattcc
     2821 cacaggaatt atctgcggca ttgcaggtat gtatgtaacc tttaccttca taaatacctt
     2881 aattcaggga gtttttggga tagcagaaag gcttagggtt atagtaacac ccctgtcaat
     2941 cctggttgca tctttggtct ctgctattac aatcctaatc tcggcctata tccccgccaa
     3001 aagggcatcc aaggtttccg ccattgatgc cataaggcaa tcgatggacg ttagattgac
     3061 ccgcaaagcg gtaaagacat caagaaccgt tcgcgcccta tttggaatcg aggcagaaat
     3121 cggccttaaa aacctaaaga gaaacaagcg cagatatagg gctacggtct tttccctgat
     3181 cgtcagcatc atcctcttct taacggtatc cttctttacc aatgatctga ggaaggctgt
     3241 acgaatgacc caggccagaa taaattatga tatttatatg aacgctaacg tggatattga
     3301 cgatggttct atagagtcca ttaaatctct gccggacata tacgaatata gccgggttcg
     3361 taagctctac acatattctt ggattgaaga ggaatttgta cccgaagggc taagggatgg
     3421 gggaaccgaa tttagggatg ggcgatatct ctatgggata gaggtgcagg ctttagatga
     3481 tgacagcctg tccaggtttg caaaaatagc aggggctgac tacgaaaagc tcatagaccc
     3541 aaatgatctg tccgctattg ttatcgatac cataacctac cccgatgaag gcatgggcaa
     3601 attcgtcgag acaaaagcca taaaagcgag ggtcgggcac aaactggatt ttattaacat
     3661 tgattgggaa agggaagtgg aaacggatat caattcggtg aagatcatag ctttaacagg
     3721 tgaatcccca atgggggttg atccctctcc cttaggtgtt atcaatattg tgatctctga
     3781 aaggttaatg gatagctttg taggcagcgg agaaaacgat ccgggatttt gggataacct
     3841 ctatttaagg agtaataatc ccataaagac ccaagaggag atagagaaga tacaggcagt
     3901 cggcctgtat atccataatg tatataagca aaggcaagag gaggaacagc taattttgct
     3961 gatgtccgta tttatatacg ggtttattac tttaataacc gctatatcca tagccaacat
     4021 ctttaacacc atatccacca gcatcgtgct tcgcaaaagg gaattcgcca tgctaaaatc
     4081 cgttggtatg acgccaaaga gcttcaacag gatgataaac tatgaaagta tattttacgg
     4141 aataaaatcc ttgttgtatg ggctgcccat aagcgttgct gtaatgtttt taatccactg
     4201 ttccatggag gatagctttg aatttgggtt tagcctgcct tggagggatg tagtcatagt
     4261 aattgtagca atatttgtta tagtcggctc tacaatgctg tattctggag gaaagataaa
     4321 gaaggaaaac atcatggatg ggctgaaaca ggagattata tagtcaaaat tccaaaaaac
     4381 acattattat aggagaggct aataatagtg tgttttttta atgctcctat cgaaacatca
     4441 atttaacctg ttgtgttagt t
//
LOCUS       DASRNX010000242        11107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_264109,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000242 DASRNX010000000
VERSION     DASRNX010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11107
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264109"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..746
                     /locus_tag="VFD89_09705"
     CDS             63..746
                     /locus_tag="VFD89_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HZJ58492.1"
                     /translation="MLNIFFRSLILYAIVVLVVRVMGKRQVGQLQPFELVIAIMIAEL
                     ASIPADDVGIPLVKGLVPIFALAFAQITISFLSLKSEKFRALVGGSPSILIENGVIEQ
                     KKLEQLRINLNDLLEQLRSKNIANVDDVEFAILETNGNLSVFPKSDKNNATLEDLNIQ
                     GKPEDIPMTLVMDGQIMHNNLKKTGQDLSWLLNQIKALGLDRQSVFFAYLDTQNELKV
                     IAKQKEQAR"
     gene            743..1123
                     /locus_tag="VFD89_09710"
     CDS             743..1123
                     /locus_tag="VFD89_09710"
                     /inference="COORDINATES: protein motif:HMM:NF025634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HZJ58493.1"
                     /translation="MRTFIIILIVLVLFLGLSWWNTSYLTRTSKELASHAEKIRTTVT
                     DENWEHANDEVMQIRRTWGNHKKTWLLLVDHEDIDDIDKTIYRIDELVKQKEKEQALA
                     DIAELRFFVQDLADKEVLSLANIF"
     gene            complement(1280..4114)
                     /gene="uvrA"
                     /locus_tag="VFD89_09715"
     CDS             complement(1280..4114)
                     /gene="uvrA"
                     /locus_tag="VFD89_09715"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZJ58494.1"
                     /translation="MEAPKIFVKGARENNLKNIDLEIPRNKLIVFTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMEKPDVDYIEGLSPAISIDQKTTSHNPRSTVGT
                     VTEIYDYLRLLFARIGIPHCPNCGRVIEQQTVEQMVDIVMALEERSRIQLLAPLVRGR
                     KGEYTKLLDQIRKDGYVRVRIDGEMMELSEEINLDKNRKHTIEVIVDRLIIREGIAQR
                     LADSLETVLNLSGGLAIVNIIDGEEILFSQNYACPECNINIEELTPRMFSFNNPYGAC
                     ETCSGLGSMMEIDPDLVIPNKNLSLAEGAIVALGWNNLSEDSIAFMYLKALSRQYNFD
                     LNTPIKKLSKKVIDIILYGTKGKKFNIDYSRNDGSGSFAGSFEGIINNLQRRHRETQS
                     DYSRDYIERLMSNRTCPACHGARLKPEVLAVTVGDKNIYDITTTSIKESRDFFNNLNL
                     TSKQEMIGHQIFKEIQARFGFLVDVGLDYLTLSRSAGSLSGGEAQRIRLATQIGSGLV
                     GVLYILDEPSIGLHQRDNDRLIRTLKGLRDLGNTLIVVEHDEETMLSADHIVDIGPGA
                     GNHGGEVVAMGSPSEIMKDDKSITGQYLSGKKRIDIPEVRRKPNDKYLEISGARENNL
                     KDIDVKIPIGVMTCITGVSGSGKSSLVNEILYKALARELNRARIRAGDYDKILGTQYL
                     DKVIDINQSPIGRTPRSNPATYTGVFDGIREVFAMTNESKVRGYRVGRFSFNVRGGRC
                     EACQGDGIIKIEMHFLPDVYVPCEVCRGKRYNRETLEVRYKGKNISDILNMTVEEALG
                     FFENIPKIRRKLETLNDVGLGYIKLGQPSTTLSGGEAQRVKLATELSKRSTGQTLYIL
                     DEPTTGLHAADVHMLIGVLQRLAEGGNTIVIIEHNLDIIKVADYIIDLGPEGGDRGGM
                     VIGAGSPEELAGMENSYTGQFLRKVLERGY"
     gene            complement(4243..6210)
                     /gene="uvrB"
                     /locus_tag="VFD89_09720"
     CDS             complement(4243..6210)
                     /gene="uvrB"
                     /locus_tag="VFD89_09720"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZJ58495.1"
                     /translation="MDFKIRSAMKPRGDQPKAIASLVEGLNKGEKYQTLLGVTGSGKT
                     FTMANTVEKVQRPTLVLAHNKTLAAQLYSEFKEIFPDNAVEYFVSYYDYYQPEAYMPV
                     SDTYIEKDASINDEIDKLRHSATSALFERRDVIVVASVSCIYGLGNPAEYRDLTVSLR
                     VGMNRERNEIIRMLVDIQYERNDINFIRGTFRVRGDVLEVFPASSSEHAMRVEFFGDE
                     IDRISEVDVLTGEILGLRNHVAIFPASHYAASRERLEKAIGMIEVDLEERLEEFRAQD
                     KLLEAQRLEQRTNYDIEMMREIGYCSGIENYSRYMDGRQIGEPPFTLLDYFPDDFLLI
                     VDESHVTFPQVRAMYAGDRSRKDSLVEYGFRLPAAYDNRPLRFEEFEERICQVVCVSA
                     TPADYELSHSSRVVEQIIRPTGLIDPKVEVRPIEGQIDHLIGEIQKITNRNERVLITT
                     LTKKMAETLTSFLKEMDMKVRYLHSDIVTLERVEIIRDLRMGVFDVLVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSETSLIQTIGRAARNVQGKVIMYADTITGSMERAIDETN
                     RRRELQIEYNRLHGIVPTTIVKEIHDIIEATHAAEEGPGYDISIPETREQILQIIADL
                     EKDMQNAAIALEFEKAAKLRDEIFKLKNELS"
     gene            complement(6476..7609)
                     /locus_tag="VFD89_09725"
     CDS             complement(6476..7609)
                     /locus_tag="VFD89_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZJ58496.1"
                     /translation="MQLLKQLFTSYSQVISSFFYIGIVLVVYLRIQRNAQLEEAWLGI
                     LRNSINTQLYYVLLYGLIGGLLTSFIILIFEIRIEFEAMLIIWPISLFLMLFNPRYLC
                     PSYSIGIVSLMSLIFGWPAMEVSPMIMLVGILHLAEGLLILIDGARDSIPVYIEHDRF
                     TPAGAHIMNRMWPIPLATITAPGQLVFPAVAVLVYEDWAITQTTHRRVRESGFWAGIY
                     GLTLLILAMASIRIKGLTYLAVLATLAMHEIITRWNRQDRLKGEPIFKAPWRGIRVLD
                     VLPESPGKAMGLMQSDIILYMNGRQVNSETMLEEILRDSPNFIWVTILRGDGETLELE
                     YRDYERGIKDLGLIFVPRTTAKYYIFKHPKGILVRLWDNYFKG"
     gene            complement(7705..8952)
                     /locus_tag="VFD89_09730"
     CDS             complement(7705..8952)
                     /locus_tag="VFD89_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZJ58497.1"
                     /translation="MISKKSAALGAVVLILVTVLFTAFLTIQVGTYIDIRNGDRVIVS
                     RQDYQLLNTYYRKLETVRRDIEANYIEETNREDLFEGALKGMAASLEDPYSYYLTDEE
                     LEDFNIATSGTYQGIGVSIEKSEDNLIMIFQVFKNSPAMEAGLLTGDKIIKVEGKDVD
                     WSMYEQAVAMMKTGDPGSVVEIAILRDGEVKDFSIRRDIVEMPDMEYDMLENDIGYIW
                     LYSFDGVAAKNFKNAVEDLESKGMKGLVLDLRGNGGGLLEVCREIADVLLPEGLVVYS
                     ESRSGQRTEYKSDPEELGIPMAVLVNEYSASASEVLAGAVQDYGVGTIIGTTTFGKGV
                     VQTMKPYEDGGALKLTTFKYFTPKGRDINHKGIEPDIKVEMTEEAKNFLKENPRENLP
                     TQMDAPLLKGIEEILSELGESTQ"
     gene            complement(9321..10646)
                     /locus_tag="VFD89_09735"
     CDS             complement(9321..10646)
                     /locus_tag="VFD89_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZJ58498.1"
                     /translation="MKRIVTWVCVLVFLLSATTTVLANEIEQHRNEAKEVNQDIDETQ
                     GQLDRVKGEQAKVSNQLEEIDRDINGKEKELIHIQGRLDDTTALLDETKQELKIAEAE
                     ADRFEGLMADRLCAMYMMEGTSYLELLFGASNLNELLDRLEMVKFMITFDNQVFDRMM
                     ELQSAIVEKKEDLEIQEETIKETKDEISHQKSLIEAKKSERLQVMIRLREEEHQYEKD
                     LEVLEQTSKDLEKTIQRLLKEQEQRKREEEQKRKEEEQRRREEEQKRNEEEQKNKDTE
                     QGKKEEEGKKEEQDKGGGQQEQKDQDRGDSKGNASMVWPAPGFTHISSGYGNRWHPVS
                     GQYKFHTGIDISGGGINGKDAVAAAGGTVILSQVYGGYGNCVIVDHGDGITTVYAHGS
                     KLLVSVGQKVKSGQAVMKIGSTGVSTGPHLHFEVRKNGSHVSPLPYLGR"
     gene            complement(10701..>11107)
                     /locus_tag="VFD89_09740"
     CDS             complement(10701..>11107)
                     /locus_tag="VFD89_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZJ58499.1"
                     /translation="TSRIIGLWLMILLILVAMIIINNTIKITIYSRRREINIMKYIGA
                     TDGYIRWPFIIEGFTLGIFAAVLAGILVIGGYNLLLGRSGQLSSNYSLLSLFQPLPME
                     GMIYDVGLIFLLVGSGVGIIASFFSTRKYLRV"
BASE COUNT         2750 a         2953 c         2165 g         3239 t
ORIGIN      
        1 tagattttgt agttggagtt gtttatctat atggatcata ccattagaag gagatactcc
       61 cattgcttaa tattttcttt agatccttaa ttctctatgc catagttgta ttggtagtta
      121 gggttatggg caagcgacag gttggccaat tacaaccctt tgaactggtc atcgccataa
      181 tgatagcgga gttggccagc atccccgccg atgatgtggg catacctttg gtcaaagggc
      241 tggtgcccat atttgctttg gcctttgccc agataaccat ctcgttttta tccctaaaga
      301 gtgaaaaatt tagggctctg gttggcggct cccccagtat cctaattgaa aacggcgtta
      361 ttgagcaaaa aaaattagaa cagcttcgaa tcaacctaaa cgatctcctg gagcagctga
      421 ggtcaaagaa tattgccaac gtggatgatg tggaatttgc tatactggag accaatggca
      481 acctttccgt cttccctaaa agcgataaaa ataatgctac tctggaggac cttaatattc
      541 agggcaagcc ggaggatata cccatgacct tggttatgga tggacaaata atgcataata
      601 accttaaaaa aaccggtcag gatttaagct ggttgttaaa tcagataaaa gccctgggtc
      661 tggatagaca gagcgtattc tttgcctatc tggacaccca aaatgagcta aaggtgattg
      721 caaagcagaa ggagcaagcc cgatgagaac ctttattata attctgatag tcctagtgct
      781 atttttaggc ctatcctggt ggaatacctc ctatcttacc aggacatcca aggaacttgc
      841 atcccatgcc gaaaaaatcc gcaccactgt gacagatgaa aattgggagc atgcaaatga
      901 tgaggtaatg caaattcgcc gtacatgggg taatcataaa aaaacctggc tcttactggt
      961 agatcatgag gatatagatg acatcgataa aaccatatac aggatagatg aactggttaa
     1021 acaaaaagaa aaggaacagg cactggcaga tatcgcagag ctcagattct ttgttcagga
     1081 tcttgcggac aaagaggttc tgagtctggc taatatcttt tagtgccatt tcaattcacg
     1141 ctttgaaagt ttcaattaga tcagtaccca cggttaagat taatacccac gttgaaatcg
     1201 atacccgcgt ttggggtcaa tacccgcatc tgaagccggt gcccgcatat gggattagta
     1261 cctatgccct tgcctgagat caatacccac gttccaatac cttcctgagg aactgtccgg
     1321 tatagctgtt ttccatcccg gccaattctt cggggcttcc ggcacctatt accatgcctc
     1381 ccctatcccc gccttccgga cccaggtcga ttatataatc tgctactttg attatatcca
     1441 gattatgttc aataattact atggtatttc cgccctccgc caacctctgc agcacaccta
     1501 tcagcatatg gacatcggct gcatgaagcc cggtagtggg ctcgtccaaa atatataggg
     1561 tctgaccggt gctgcgcttg ctaagctccg ttgccagctt aaccctttgg gcttctcccc
     1621 cggacagggt ggtggacggc tggcctaatt ttatatatcc cagccccaca tcgttcaagg
     1681 tttccagctt tcttcggatc ttgggtatat tttcaaaaaa gcccaatgcc tcttcaacgg
     1741 tcatattaag gatatccgat atgttcttac ccttgtagcg gacctccaaa gtctccctgt
     1801 tatatctctt acccctgcat acctcgcagg gtacatagac gtcgggcaga aaatgcatct
     1861 ctatctttat gattccgtcc ccctggcaag cctcacagcg accccctcta acgttaaaac
     1921 tgaacctgcc cacccggtat ccgcgaactt tggattcatt ggtcatggca aaaacttcac
     1981 ggatcccgtc aaatacccct gtataggtag ccgggttgga tctgggcgtt cttcctatag
     2041 gtgattgatt gatatcaata accttgtcca ggtattgggt gcctaaaatt ttatcatagt
     2101 ccccggccct aatccttgca cgatttagtt ccctggccag ggccttgtaa agtatttcat
     2161 tgaccaggga gctctttcct gaaccggaaa ccccggttat acaggtcatt acccctatgg
     2221 gtatttttac gtcaatgtcc tttaaattat tttctcttgc cccactgatc tcaagatatt
     2281 tatcattggg ttttctcctg acttcaggaa tatctatccg ctttttcccg gatagatatt
     2341 gtccggttat agatttatca tctttcatga tttctgacgg gctccccatg gccaccactt
     2401 ccccgccatg atttcccgcc ccgggtccga tgtcgactat atgatcggct gagagcatag
     2461 tttcctcatc atgctcgact actataagag tattgcccaa atcccttaac ccctttaggg
     2521 tccttataag cctgtcgtta tcccgttgat ggagacctat gctgggctcg tccaggatgt
     2581 atagaacccc caccaggccg gagcctattt gagtcgccag ccggattcgc tgtgcctctc
     2641 ccccggagag gctgccggcc gacctggaca gtgtcagata atccagtcct acgtcgacta
     2701 gaaatccaaa ccttgcctgg atttccttga aaatctgatg ccctatcatc tcctgtttgg
     2761 aagtaaggtt caaattatta aaaaaatcac gggattcctt tatggatgtg gtcgtaatat
     2821 catatatatt cttgtccccc acggtcacgg ccaaaacttc cggttttaac ctggctccat
     2881 ggcaggcagg gcaggtcctg ttgctcataa gcctttcaat atagtccctg ctgtagtccg
     2941 attgggtctc cctgtgtctt ctttggaggt tgtttattat cccctcgaag gagcccgcaa
     3001 atgatccgga accgtcattt ctgctgtaat caatattgaa cttcttcccc ttggttccgt
     3061 agaggatgat gtctataacc ttcttgctca gcttcttaat tggggtgttc aagtcaaaat
     3121 tgtattgcct gctaagagcc ttcaggtaca taaaggcgat gctgtcttca cttaaattat
     3181 tccatcccag ggctacgata gccccctcag ccagggatag attcttattt gggataacca
     3241 gatccgggtc tatctccatc atagaaccaa gcccgctaca tgtctcacag gccccgtagg
     3301 ggttattaaa ggagaacatc ctgggggtca attcctctat gttaatattg cattcgggac
     3361 aggcataatt ttggctaaag aggatctcct cgccatcaat tatattcact atggccagcc
     3421 cgccgcttag attcagtacg gtttccagag aatccgccag ccgttgggca ataccctccc
     3481 ttatgatcag tcgatctact atgacctcta tggtgtgctt cctattttta tcaaggttta
     3541 tctcttcgga aagctccatc atctcgccgt ctatgcggac cctgacatag ccatccttcc
     3601 ttatctggtc caatagcttt gtatactccc cttttcttcc cctgacaagg ggagccaata
     3661 gctgtatccg gcttcgttcc tccaacgcca tgacgatatc taccatctgc tccacggtct
     3721 gctgctcgat taccctgccg cagttcgggc aatggggtat acctatcctg gcaaacagga
     3781 gccttaggta gtcataaatc tcagttactg tacccacggt ggatcttggg ttatggcttg
     3841 tagtcttctg atctatggag atggccggcg ataaaccttc aatatagtca acgtcgggtt
     3901 tttccatctg ccctaaaaac tgtctggcat aagcggaaag ggattccaca tatcttctct
     3961 gaccctctgc gtatagggtg tcaaaggcca gggaggactt acccgaaccg ctgagcccgg
     4021 taaacacaat cagtttattt ctgggtattt ccagatcaat attcttaaga ttattttccc
     4081 tggccccttt tacgaatatt tttggtgctt ccatcttctg tctccttctt gtctcagtat
     4141 gcggcatggg tatcacagaa tatctaatag gcctacccaa gattcaaaaa gataatgtat
     4201 ttttgaaatc tttctgtcaa ggcccataag aatcctacgc ccctaactca attcattctt
     4261 taatttgaat atctcatccc tcagcttggc cgccttttcg aattctaagg caattgccgc
     4321 attttgcata tccttttcca aatcggcaat gatttgaagt atttgctccc gggtttccgg
     4381 tatagagata tcgtatcccg ggccttcctc tgccgcatgt gtagcctcta taatatcgtg
     4441 tatttcctta acgatggtag taggcactat accgtgtaac ctattatatt ctatctgcaa
     4501 ctctcttctt cggttggtct cgtcgatggc cctttccatg gatccggtga tggtatcagc
     4561 gtacattatt accttgccct gcacattcct ggcggcacgg ccgatggttt gaatcaaaga
     4621 tgtctcggac ctcagaaaac cctccttatc agcgtctaat atcgctacca gggaaacctc
     4681 cggcaggtcg aggccctccc tgagaagatt aattcctacc agcacgtcga atacccccat
     4741 ccgcagatct ctgataattt ccaccctttc aagggtcacg atgtccgaat ggagatatct
     4801 taccttcata tccatttcct tgagaaagct ggtcagggtt tctgccatct tcttggtcag
     4861 agtagttata aggacccttt catttctgtt tgtaatcttt tggatctctc cgatcaaatg
     4921 atcaatctgt ccttcaatgg ggcgaacctc gaccttggga tctatcagcc ccgtcggcct
     4981 gataatctgc tccacgaccc tggatgagtg ggatagctca tagtctgcgg gggtggcgct
     5041 gacacagacg acctgacaaa tcctctcttc aaattcttcg aacctaagag gcctgttgtc
     5101 atatgccgcc ggcaacctaa atccgtattc taccaggctg tccttcctgg atcgatcccc
     5161 ggcgtacatg gcccttacct gcggaaaggt tacatgggat tcatctacaa tcagtaaaaa
     5221 atcatcgggg aaataatcca gtaaggtaaa gggcggttcg ccaatctgcc ggccgtccat
     5281 gtatctggaa tagttctcta ttccgctaca atacccaatc tcgcgcatca tttcaatgtc
     5341 ataattggtc ctctgctcca gccgttgagc ctccagcagc ttatcctgtg cccgaaattc
     5401 ttctaacctt tcctccagat ccacctctat catccctatg gccttttcca acctctccct
     5461 ggatgcagca tagtgagatg ccgggaagat ggccacatgg tttcgaagcc ccaagatctc
     5521 ccccgtcagg acgtccacct cggatatcct gtctatctca tcgccaaaaa attctaccct
     5581 cattgcatgt tccgacgaag atgcggggaa aacttctaaa acatcccccc gtaccctgaa
     5641 ggtacccctt ataaagttta tatcatttct ttcgtactgt atatccacca gcattctgat
     5701 tatctcgttg cgctccctgt tcatacccac cctcaaggac accgttaagt ccctatactc
     5761 tgcgggattt cctagcccat agatacagga tacgctggca acaactatta catcccgccg
     5821 ctcaaataac gccgaggtag ccgagtgcct tagcttatca atctcgtcgt tgatagaggc
     5881 atccttttct atataggtat cgcttacagg catatacgcc tcgggctgat aataatcata
     5941 atagctaacg aagtattcca cggcattgtc ggggaagatc tccttaaatt cgctgtatag
     6001 ctgggctgcc agggtcttat tatgtgccag taccaaagtc ggcctctgca ccttctcgac
     6061 ggtattggcc atggtaaatg tctttcctga cccggtaaca cccaaaaggg tttgatattt
     6121 ctcaccctta tttagcccct caaccaatga ggcgatggct ttgggttggt cgccccgggg
     6181 tttcatggcg gatcttatct taaaatccat ccacctcacg ctccttttta ttatatagtt
     6241 ttaagtccca tctcggtaac caaatacaag ctgatcccca aactttaatt atgataccga
     6301 tatggaaaaa ttcaatgggt caattttgtt cctacgaagt gactccggaa tcattataac
     6361 atatccggcc gaagaataga acgcctgttc gaaaaaataa aaaacctcac ccatgctatt
     6421 cctatttctt cagggtgaag ttaaaagtca tgcctattta aatcggtatc ggaccctacc
     6481 ccttaaaata attgtcccat agtctcacaa gtataccctt cggatgctta aatatatagt
     6541 acttagccgt ggtgcgggga acaaatataa gccctaaatc ctttattccc ctttcgtagt
     6601 ccctgtattc cagttccagg gtttcaccgt ctcccctcag tatggtcacc catataaagt
     6661 taggtgaatc ccttagaatc tcttcaagca tcgtttcgct atttacctgt ctgccgttca
     6721 tatataatat tatatcgctc tgcataagtc ccatggcctt gcccgggctt tccggcaata
     6781 catccagtac ccttatacct ctccaaggcg ccttaaaaat cggctcgcct ttcagtcggt
     6841 cctgtctatt ccaacgggtt atgatttcgt gcatagctag agtggccaag accgccagat
     6901 atgtgagccc ctttatcctg attgatgcca tagccaaaat taatagagtt aatccataga
     6961 tccccgccca aaacccggat tccctaactc tcctatgggt agtttgggta atggcccaat
     7021 cctcatatac caataccgcg actgccggaa acaccagctg tcccggcgct gtaatcgtag
     7081 ccaagggaat tggccacatc ctgttcataa tatgggcgcc tgccggtgta aaccgatcat
     7141 gctcaatata taccggtatg ctatctctgg ctccgtctat cagtataagg agaccctccg
     7201 ccaggtgcag gataccaacc aacatgatca tcggcgaaac ctccatggcc ggccatccaa
     7261 aaataaggga catcaaggat acgatcccta tggaatagga ggggcatagg taccgcggat
     7321 taaaaagcat caaaaatagg gatatgggcc aaattataag catagcctca aactctatcc
     7381 ttatctcgaa gattagaatg ataaagctgg tgagcagccc ccctatcaga ccatagagga
     7441 gcacgtagta tagctgggtg tttatagaat ttcgaagaat gcccaaccag gcttcctcca
     7501 gttgggcatt cctttgtatc ctcagataaa ccaccagaac aattccgata tagaaaaaac
     7561 tgctgatcac ctgtgaatag gaagtaaata attgttttaa caattgcatt ttatgcctcc
     7621 taaaacatat aactgggaca ggaaatccaa ccctaccttg gtgagggcca taaatctgca
     7681 tctatatcgg aaatatgacg atatctattg tgtagactcc ccaagttccg ataggatttc
     7741 ttcgatccct tttagcaggg gagcatccat ctgggtaggc agattttccc tcgggttctc
     7801 ttttaaaaag ttttttgctt cttccgtcat ctctaccttt atatccggtt cgataccctt
     7861 atgatttata tcccgtcctt taggagtaaa atatttaaag gttgtcagct ttaaggctcc
     7921 cccgtcttca tagggtttca tagtttggac cacacctttt ccaaaggtcg tggtacctat
     7981 tattgttcct acaccatagt cttgaacagc ccccgccaag acttccgatg cgctagcgct
     8041 atactcattt accaatactg ccatggggat ccccagctcc tcaggatccg atttgtattc
     8101 ggtcctttga ccgctgcggc tctcactata cactaccaac ccttcaggca acaacacatc
     8161 agcgatttcc ctgcatactt ccaggagccc cccaccgttt ccgcgaagat ccaatacaag
     8221 ccccttcatg cccttacttt ccaggtcttc aacggcattt ttgaagttct tggccgcaac
     8281 gccatcaaag ctatagagcc agatgtaccc aatgtcgttt tcaagcatat catattccat
     8341 gtcgggcatt tcgactatat cccttcgtat ggaaaaatct ttgacctcgc catccctcaa
     8401 aatagcaatc tcaacgacgc ttccgggatc ccccgtcttc atcatggcta cggcctgctc
     8461 gtacatgctc caatccacgt ctttcccctc tacctttatt atcttatccc cggttaacaa
     8521 tcccgcttcc atggccggac tgtttttaaa cacctgaaat atcattataa ggttatcctc
     8581 tgatttttca atagacacac cgattccctg ataggtaccg gaagtcgcaa tattgaaatc
     8641 ttccagctcc tcatcggtaa gatagtagga atacggatct tcgagggaag ccgccatacc
     8701 ttttagggcc ccttcaaaaa gatcctcgcg attagtttcc tctatatagt ttgcttctat
     8761 atcccttctt acggtttcca acttcctata gtaggtatta agcagctgat agtcctgcct
     8821 tgatactatt accctgtctc catttctaat atcgatgtat gttcccactt ggatagtcag
     8881 aaaggcggta aataaaactg ttacgagtat aagtacaact gcccctaagg cggctgattt
     8941 tttactgatc atacattaga tcctcctaac cccgagtttt cataacccat aggtagtttt
     9001 tcccttgccg aagctgcaac agcctgggca agggaataaa tcatatccta ttatagcact
     9061 aatcccacaa atttcaaaac taattttgat gcctccgggg taactcccca cccatataaa
     9121 acaagccgga agccggattg gtggtgatat acttttcaag atgaggatga aggtcagttc
     9181 atctgtagga ataagacgaa aaacccacgg tttcaatcgt gggacgtaag cccatgttac
     9241 ggcacaaaaa tagggacagt tttttattcc tgtcccctgc ataatccatt ttataaacca
     9301 ccagaatcag cgcaccgctg ttatcttccc agataaggca acggactcac atgagagccg
     9361 tttttccgca cctcaaagtg tagatgaggc cctgtagaaa caccggtgct gccaatcttc
     9421 ataacagctt gtccgctctt taccttctga cccaccgaaa ctaacaactt ggaaccgtgg
     9481 gcgtataccg tggttatacc gtccccatga tctacgataa cgcaattccc gtatccgccg
     9541 tatacctgag acagtattac ggtaccgccg gcagcggcaa cagcatcctt gccgttgatc
     9601 cccccgccgc ttatgtctat tcccgtatgg aacttatatt gtccgctgac gggatgccat
     9661 ctattcccat atccggatga aatatgggta aagcccggag caggccatac catggaggcg
     9721 ttgcccttgc tgtccccccg atcctgatcc ttttgttcct gttgcccgcc gcctttatcc
     9781 tgctcttcct ttttcccttc ctcttctttc ttcccttgct ctgtatcctt attcttttgc
     9841 tcttcttcat tacgcttttg ctcttcttcc ctgcggcgct gttcctcctc tttacgcttt
     9901 tgctcctcct cgcgtttcct ttgttcctgt tcctttagaa gcctttggat ggtcttctcc
     9961 agatccttag aggtttgttc caacacctcc agatcctttt cgtattggtg ttcttcctcc
    10021 ctaagccgga tcataacctg gagcctttcg ctcttctttg cttcaatgag actcttctga
    10081 tgggaaatct cgtccttagt ctccttgatg gtctcttctt ggatctccag gtcctctttc
    10141 ttctccacta tggcactctg caattccatc atccggtcaa atacctgatt atcaaaggta
    10201 atcatgaact tgaccatttc aaggcgatcc agtaactcgt ttagattgct tgcgccaaat
    10261 aaaagctcca ggtaagaagt tccctccatc atatacattg cacaaagccg atcagccatc
    10321 aagccttcaa acctatcggc ctccgcctcg gcaattttta actcttgttt tgtctcatcc
    10381 aatagagccg tagtatcgtc cagcctccct tgaatatgta tgagttcctt ttccttgcca
    10441 tttatatctc tatctatttc ctccagctgg ttggatacct tggcctgttc ccccttgaca
    10501 cggtccaatt gcccctgggt ttcgtctata tcctggttta cctcttttgc ctcattcctg
    10561 tgttgctcta tttcatttgc cagcaccgta gtcgtcgcag acaatagaaa taccagcaca
    10621 cacacccatg tcactatgcg tttcacatta acaccccaca aacatttttt atcccgtaca
    10681 ctttacccca ttgttcagtc ctacaccctg agatattttc tggtcgagaa aaagctggca
    10741 attattccaa ccccgctacc taccaagagg aagataagcc ccacatcata tatcatacct
    10801 tccatgggca agggctgaaa taagcttaaa agactgtagt tgctcgatag ctggccactt
    10861 ctacccaaca ataggttata tccgccgata accaaaatcc ccgctaaaac agccgcgaat
    10921 attcccaacg taaatccttc aataatgaat ggccagcgta tatatccatc tgtagcaccg
    10981 atatatttca taatattaat ttccctacgc cgggaatata tggtgatctt gatggtatta
    11041 tttattatta tcatggcgac tagaatcaat agaatcataa gccacaagcc tattatgcgc
    11101 gaggtct
//
LOCUS       DASRNX010000243         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_368759,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000243 DASRNX010000000
VERSION     DASRNX010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368759"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="VFD89_09745"
     CDS             complement(<1..1215)
                     /locus_tag="VFD89_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HZJ58500.1"
                     /translation="MNYGIVTRVLGNLLVLEALLMAPSLLVSIIYGQNDLYAFLISMA
                     IMIFLGLIMSRFGASNKKIKAREGLTIVALGWILASFFGSLPFVISGSIPSPIDAFFE
                     TMSGLTTTGSTLVGNIEVLPKGILFWRSFTHWIGGMGILVFTVAILPTMGIGGFQIFK
                     AESPGPTADKIVPRIKDTAKILYITYISITVLQIVLLVVGGMSLYESAVHTFGTVGTG
                     GFSTRNASIGAYKSTYIHVVITVFMIMSGASFSLYYGLVKGKWRDVIRNGELRLYLGI
                     ILASGIFIGLNLFIGTGAYKSIGLAFRDSFFQVGSIITTTGYATVDFDLWPAFSKMIL
                     LFLMFVGGCAGSTSGAIKNIRVLVLFKLIKRQVVKTFHPNAYTPIKVDGKVLNDDTVT
                     GISSFFVLYIIMF"
     gene            complement(1166..2572)
                     /gene="trkA"
                     /locus_tag="VFD89_09750"
     CDS             complement(1166..2572)
                     /gene="trkA"
                     /locus_tag="VFD89_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HZJ58501.1"
                     /translation="MKTMIIGAGKLGYKLAELMINENIHVTLVDSNSKVIERISDHLD
                     VLTVIANGLELETLKELGIESYDLLIAATSSDETNVIICSIAKKLGCKKTIARVRDPE
                     YIHQFGFIKSEMGIDHIVNPDLATANEISRYLLKSYSYNNGEFAKGRVSMVDFHVNNM
                     REFIGKRLMDLDGFEGLLVVAVSRDREIIIPHGGTVLEEDDIIHIMGESKSINNLAAR
                     FKLNMDKRQIKRVMILGGGKISHYLADQLIQANIRVTILEQSRERCRYLSEKLDDALI
                     INGEGTDINLLEEENLGEMDAFVGATGYDEQNLLMSLMAKQAGVNSVIAKISRPSYVH
                     LIEKLGIDFALDPTNIIASDILKFIRGGKVVSVSLLLGEQAEVMEVIVDEKVTAIGKP
                     LSDLGMPKGIIIGAIVRDRRVIIPNGRTVIYPKDRLIIFCLASNVPYLKTFTTPSKGG
                     VFSELWNRNQGTGQSIGS"
     gene            complement(2879..>3084)
                     /locus_tag="VFD89_09755"
     CDS             complement(2879..>3084)
                     /locus_tag="VFD89_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HZJ58502.1"
                     /translation="ATSLGAAIAAGVGVGIFKDFEEAEGVIEFSRDLKPHSETVKEYR
                     RYYEVYKMMYPALKPVYDAISRL"
BASE COUNT          813 a          779 c          622 g          870 t
ORIGIN      
        1 gaacattatg atataaagca caaagaaact tgatatcccc gtaacggtgt catcgtttaa
       61 gaccttgccg tcaaccttta taggcgtgta ggcattggga tgaaaggtct taaccacctg
      121 tctttttatt agcttaaata ataccaaaac ccgaatattt ttgatagcgc cgctagtgga
      181 gccggcgcat ccgcctacaa acataagaaa caagaggatc atcttgctaa aggccggcca
      241 aaggtcgaaa tcaacggtag catagcccgt agtggttata atggacccca cctggaaaaa
      301 ggaatccctg aaggcaaggc ctatactttt ataggcccct gtacctataa acaggttaag
      361 ccctataaag atgcccgagg ccaggattat tccaaggtag agcctcagct ccccgtttcg
      421 tattacatcc ctccacttac ccttaaccag cccatagtag agggaaaagc tggcccccga
      481 cattatcata aacaccgtaa taaccacgtg gatataggtg ctcttgtatg cccctatact
      541 ggcatttcgg gtggaaaatc ctccggttcc cacggttccg aaggtgtgta cagcggattc
      601 atagaggctc atacccccga ctacaaggag tacaatctgc aggaccgtta tagagatata
      661 ggttatataa agtatcttgg cggtatcctt gatccttggg actatcttat cggcggttgg
      721 cccggggctc tcggccttaa atatctgaaa gccaccaatg cccatggtag gtaggatagc
      781 caccgtaaat accaatatgc ccattccccc tatccagtgg gtaaaggagc gccaaaatag
      841 aattcccttt ggcaacacct ctatatttcc caccagggta gagccggtgg tggttagtcc
      901 cgacatggtt tcaaagaaag catctatggg ggatggtatg ctcccggaga taacaaaggg
      961 gagggaacca aaaaaggagg ctagtatcca cccaagggcc actatggtca ggccctccct
     1021 ggccttgatt ttcttgttgg atgccccgaa cctggacata ataagcccca aaaagatcat
     1081 tattgccatg gatattaaga acgcatacag atcattttgg ccatatataa tggataccaa
     1141 aagagagggg gccataagca gcgcttcaag aaccaataga ttgcccagta ccctggttac
     1201 gattccatag ttcactaaaa acacccccct tgctcggtgt cgtaaaggtc tttaaatagg
     1261 gtacgttaga tgcaagacag aagataatga gtctgtcctt gggatagata accgttcttc
     1321 cgttgggtat aataaccctc ctatccctga ctatggcacc tatgatgata cccttgggca
     1381 tccccaggtc ggataggggt tttccgatgg ccgttacctt ttcgtccacg atgacctcca
     1441 tgacctcggc ctgttcccct aaaagcaggg atacggatac aacttttccc ccacggataa
     1501 actttagaat atcgctggcg atgatattcg tagggtcaag ggcaaaatct atgcccagct
     1561 tctctataag gtgtacatag ctgggcctgc tgatcttggc tatgacgctg tttaccccgg
     1621 cctgttttgc cattagggac atcaaaagat tctgttcatc gtacccggtt gccccaacga
     1681 aggcatccat ttcccccagg ttttcttcct ccaaaaggtt tatatcggta ccttccccgt
     1741 ttataatcaa tgcatcgtcc aatttttccg acagatacct gcacctctcc cggctctgct
     1801 ccaaaatagt tacccttata ttggcttgga tcaattgatc cgccaggtaa tggctgattt
     1861 taccgccgcc taaaatcata acccttttta tttgcctttt atccatattt aacttaaacc
     1921 tggcagccag attattgatg ctcttgcttt cgcccattat gtggataatg tcgtcctctt
     1981 ccaggaccgt accgccgtgg gggatgatta tctccctatc cctagatacg gcgaccacca
     2041 acaagccctc aaaaccgtcc agatccatga gccttttacc gataaattcc ctcatattat
     2101 taacatgaaa atccaccatg gagacccttc ccttggcaaa ttccccgtta ttatagctat
     2161 agctctttaa aagataccgc gagatctcgt tggccgtggc caggtcggga tttactatat
     2221 ggtctatgcc catctcggat ttaataaatc caaattgatg gatatattcc ggatccctca
     2281 ccctggctat ggtcttctta catcccagct ttttcgcgat gctacatatg ataacattgg
     2341 tctcgtcgct gctggtcgca gctataagga gatcgtagga ttcgatccct aattccttta
     2401 aagtttcaag ttccaaacca ttggctatta ccgtcaacac atccagatga tcactgatcc
     2461 tctctatcac cttggaattg gaatctacca gggtaacatg gatgttttcg tttatcatca
     2521 gctctgctaa cttgtacccc aattttcccg caccaattat catggttttc atttggcatt
     2581 tcctttccct caatccggac ctacacaatt ttgacccctt ttagtccggc tgataacata
     2641 tatatcagta ttgtaatact atatttttta atgtcaataa atgattattg gcagaaagca
     2701 ctttagttct gcttctgcta ctgggtctgg tattaagtgg ttttattaat accggattca
     2761 gtggcagata atagcagaac ttggtttgtg ccgataatca taaatgatta ttggcagaaa
     2821 gcactttagt tctgcttctg ctactgaatc ggactgcgat gtacttatct atcttccgct
     2881 ataaccttga aatggcgtca tatacgggtt ttaaggccgg atacatcatc ttatatacct
     2941 cataatatcg cctgtattct ttgacagtct cagaatgtgg ctttaagtcc ctgctaaatt
     3001 caatgacccc ctctgcctct tcaaaatctt tgaatatccc cacacctacc cctgcagcta
     3061 tagctgcgcc caaggaggtg gccc
//
LOCUS       DASRNX010000244         8124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_51236,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000244 DASRNX010000000
VERSION     DASRNX010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51236"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VFD89_09760"
     CDS             complement(<1..1276)
                     /locus_tag="VFD89_09760"
                     /inference="COORDINATES: protein motif:HMM:NF020044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HZJ58503.1"
                     /translation="MFNIDTDAIREECFLPSTFQRGRTYYRQRRVKSLKLNPKDMIIE
                     GTVKGTRTYSVSAAFDYDGDLVETGCDCPATDRYWGPCKHIVALLLTIMEHDARGSFD
                     GLIQEKKPTPTRSASRIFDFFGQMGQWEKKKTVSMEVTYEANDNLEREDIPAGFLYLK
                     IGETRLYVVRNIPNLLKAYRDGEALVFGKNFTYDPAVHTLCSADKRLLDFMTEIYEVQ
                     GLIDSASYYRTSGLFSDRRLALTDATAKRFFSASEGRRFNLMHKGTTYKNIEITDEDL
                     PISFDLGKDEETLLLTINMGKGIYPLVRSGEYMLMGDKIYKPTEKQRIGFAPFYNALA
                     QVGSRNFRFSKEEGERFVSEVLPHVEGVGKVDIKPDLKSVIVRGVLRSEIYLRPQGDG
                     VGCDVKFVYEGREINPFGGNADIGPQGDTILIR"
     gene            1617..1946
                     /locus_tag="VFD89_09765"
     CDS             1617..1946
                     /locus_tag="VFD89_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58504.1"
                     /translation="MENQILNQILSELKGLKIGQQTINERLDIMDARLDNMDARLDRL
                     EENVAVIRADVSALRDQLLDLEAKNANNHMEMKAAIKDLSDRLSVLEVISGQNLRDIG
                     MLKAIRS"
     gene            complement(2122..3522)
                     /gene="cysS"
                     /locus_tag="VFD89_09770"
     CDS             complement(2122..3522)
                     /gene="cysS"
                     /locus_tag="VFD89_09770"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZJ58505.1"
                     /translation="MKLYNTLTRRKENFIPIYDNEVRMYCCGPTVYNFFHVGNARPFI
                     IFDAFRRYMEYKGFKVTYVQNFTDIDDKMIQRAEEEGTTVAELGERFIEEYFIDADGL
                     GIERADVYPKATEHIEEMIEFIEGLIEKGLAYVTGGDVCFDTSAFEGYGKLSRQDLEE
                     LEAGARVDPDEKKRNPMDFALWKAQKPGEPAWDSPWGKGRPGWHIECSVMASKYLGET
                     IDIHAGGQDLIFPHHENEIAQSEGLSGKPFANYWLHNGYINVDNRKMSKSLGNFFTVR
                     DIAKEYDLEIVRLFMLSSHYRSPVNFSQELLDQAKSALERLYNAQNNLLYLMDIAEDR
                     DLSAEEGKIHDSLKGYIQDFEGAMEDDINTADALSVIFDLVRDANTHLRAESPKGLIQ
                     EFYNTFKTLTGIMGIVHKPLEKTLDREIEELIEKRQQARADKDWAAADKIRDDLKERG
                     IILEDTPQGVRWTIQQ"
     gene            complement(3509..4201)
                     /gene="cysE"
                     /locus_tag="VFD89_09775"
     CDS             complement(3509..4201)
                     /gene="cysE"
                     /locus_tag="VFD89_09775"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HZJ58506.1"
                     /translation="MFKWFKDEIQGIKDRDPAARTSLEVFLCYPGFHAIIFYKIAHWL
                     HNHRLKLLARLISQYARFTTGIEIHPAADIGKRLFIDHGMGVVIGETTTIGENVTIYQ
                     GATLGGTGKEHGKRHPTIGNGVVISTGAKVLGPFTVGDNSKIGAGAVVLKEVPPNCTV
                     VGIPGRVVRKDNVPVPRDPKIRTIDCDQVDLDQVRLPDPVEDAICCIVKRLTRVEAQL
                     KELEEVRQNEVI"
     gene            complement(4530..5411)
                     /locus_tag="VFD89_09780"
     CDS             complement(4530..5411)
                     /locus_tag="VFD89_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HZJ58507.1"
                     /translation="MKKILSLGLALMLILLFPLYALADGEDSPDDEIVVSLGADLNES
                     QQKQILELFGVKRDEVKILTVTIDEERQFLGGLVSDEKIGHRTISSAYVKPLDKGQGI
                     DVETHNITWVTREMYANALVTAGVENAKVIAAAPFKVSGTGALTGIMKAFEEVSGKEL
                     DEDAKEAANEELVTTGELGEVIGKDEAAALIKEIKERIAELKTKDEGEIRKIVIEISA
                     NLNINLTDAQIEQIVKLMKKISNLDLNVEKIAGQIEKIAGSLDDVKKVVSENKGVIKK
                     ILESIEKFFAWIRNLLG"
     gene            complement(5502..5978)
                     /gene="ispF"
                     /locus_tag="VFD89_09785"
     CDS             complement(5502..5978)
                     /gene="ispF"
                     /locus_tag="VFD89_09785"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZJ58508.1"
                     /translation="MRVGIGYDVHRLADNRPLIVGGVEIPFDKGLQGHSDADVLVHAV
                     MDALLGALGKGDIGQHFPDSDDKYKGISSMDLLSVVEGIIAADGFMVGNIDGIIIAER
                     PKMAPYLKEMEENIAKILKADPSAVNIKATTTEGLGFVGVGQGIAAKAVATLEVRS"
     gene            complement(5975..6790)
                     /gene="ispD"
                     /locus_tag="VFD89_09790"
     CDS             complement(5975..6790)
                     /gene="ispD"
                     /locus_tag="VFD89_09790"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZJ58509.1"
                     /translation="MTRSRKRTVGNQIPKIRSKGLKAGDQKFEITSWELEDGLKNYAI
                     ILAAGRGTRMNREVNKVYLPLGDRPVIAHTLDAFYRADSIHRIVLVTAPGEEEYIREN
                     IFRKHPPQKPITIVFGGSERQYSVRNGLKELPKDTETVAIHDGARPLITPQVIDESVQ
                     WAVKHGAAVAGMPVKDTIKEAEPGGRVRRTLRRESLWQVQTPQTFRYSLIIEAHKKAA
                     ADDYVATDDSALAEYLGSPVYMIPGGYDNIKITTPEDIDIASRILIGRQGGQL"
     gene            complement(7007..8101)
                     /locus_tag="VFD89_09795"
     CDS             complement(7007..8101)
                     /locus_tag="VFD89_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HZJ58510.1"
                     /translation="MLRRVIRIILGLVGIAVGIALAWTSVPMLAHRGIKLIGYQDIVI
                     YILFGIAFGILFFILSKRLIQFTVDFIRLIEQKLQEIPANDILFGVSGLMIGLLISYL
                     VTIPFRQLEIGWLSLILTTLIYLFFGYLGISVTTKRWRELDWSDLLKKVQKDRHGHEV
                     FSTHPKILDTSVIIDGRIFDICKTGFVEGPLIIPAFILEELRHIADSSDALKRNRGRR
                     GLDILNQIQKELDIPVRIYEKDFDDIQEVDSKLLKLAQVLKGMVITNDYNLNKVAEFQ
                     GVPVLNINELANALKPVVLPGEEMLVQVIKDGKETGQGIAYLDDGTMIVVDGGKKHVG
                     ESIDVLVTSVLQTAAGRMIFAKPKAIDRVV"
BASE COUNT         1874 a         2088 c         1797 g         2365 t
ORIGIN      
        1 cccttataag gatggtatcg ccttgggggc ctatatccgc attgccccca aaggggttta
       61 tctccctgcc ttcgtagacg aactttacgt cgcagcctac cccgtccccc tggggcctta
      121 gatagatctc ggagcgaagt acccccctca ctatgacaga ttttaaatcc ggttttatgt
      181 ccaccttccc tacgccctcg acgtggggca ggacctcgga cacgaagcgc tcgccctcct
      241 cctttgaaaa ccgaaagttt cggctgccca cctgggccag agcattgtag aagggggcaa
      301 atccgatgcg ctgcttctct gtgggcttgt agatcttatc ccccatgagc atatattcac
      361 cgcttcttac taggggatag attcccttgc ccatattgat ggttaacaaa agggtctctt
      421 cgtccttccc aaggtcaaag cttatgggga gatcctcgtc tgttatctca atgtttttat
      481 aggtggtccc tttgtgcatc aggttaaagc gtctgccctc tgaggcagag aaaaacctct
      541 tggccgtggc atctgtgagg gccaatcttc tgtcactaaa gagcccactg gtcctgtaat
      601 agctggcgct gtctataagt ccctggacct cgtatatctc ggtcataaag tctaaaaggc
      661 gcttatcggc agagcatagg gtatgaacgg cggggtcgta ggtaaaattt ttaccgaata
      721 ccaacgcctc cccgtccctg taggccttta aaaggtttgg gatattcctc acgacgtata
      781 gcctggtttc gcctatcttt aggtatagaa agcctgccgg gatatcctcc ctctctaagt
      841 tatcgttggc ctcataggtt acctccatgg atacggtttt ctttttctcc cactggccca
      901 tctgcccgaa aaagtcaaag atccggctgg cagaccttgt gggggtaggc tttttctcct
      961 ggatcaggcc gtcaaagctg ccccttgcat catgttccat gatggttaaa aggagggcta
     1021 ctatatgctt gcaggggccc caatatctgt ctgtggcagg acaatcacag ccggtctcca
     1081 ccagatctcc gtcatagtca aaggccgcac tgacactata ggttctggtg cccttgaccg
     1141 tgccttctat tatcatatcc ttaggattaa gttttagaga ttttacccgc ctttggcggt
     1201 agtatgtcct tccccgctga aaggtgctgg gtaggaaaca ctcttccctt atggcatctg
     1261 tatctatatt aaacattgtt tcgccctttc tatcaaaaat ccattcttct attataccat
     1321 gattgagggc gtgttgtcat atttggataa aaatgatttg aattcttaaa ggatgcaaga
     1381 tcggggagaa tagagataga ataacgataa ggaaaatccg gctatacggt tacacttacg
     1441 atggtaccga tcgctcagcg cccatcatcg atatacgatt atccggtgca acacattaat
     1501 tttaagatgc ttttgaattg catatagttg ctcccagtgc tatccggtgc tataatgatg
     1561 atactacctc aaggagcgga caaactatat taaatatttt cttaggggtt gatattttgg
     1621 aaaaccagat tctaaatcag atcctttccg agctaaaggg gctcaagata ggccaacaga
     1681 cgataaatga acgactggac ataatggatg ccagattgga taatatggat gccagattgg
     1741 acagattaga agagaatgtt gcagtaatta gagccgatgt aagcgctctt agagatcaac
     1801 ttctggactt agaggctaaa aatgcaaaca accatatgga aatgaaggca gccataaagg
     1861 atctaagcga taggttatcg gttttagagg tgatatcggg acaaaacctc agagatatcg
     1921 ggatgttaaa ggccataaga tcgtagagaa tagggataga ataacgataa ggaaaacccg
     1981 gctatacgga aacgtatagc cgggtttttt tagccaaatt aatattggaa gtagcgatca
     2041 atagccattg ccgataagtc tagtatccgc attgcctatc tacagccagc ataaaaggtt
     2101 agatagatta gcagctatcc cttattgttg tatagtccag cggacccctt ggggggtgtc
     2161 ctctaagatt atgccccgct ccttaagatc gtctctgatc ttgtctgcgg cggcccagtc
     2221 cttatctgcc cttgcctgct gccttttttc gatgagttcc tctatttccc gatctagggt
     2281 tttttctagg ggtttgtgga ctatgcccat gatacccgtc agggttttaa aggtattata
     2341 aaattcttgt atgagtcctt tggggctttc ggcccttagg tgggtattgg cgtctcttac
     2401 gaggtcaaag atcaccgaca gggcatcggc ggtattaata tcatcctcca tggccccttc
     2461 aaagtcttgg atgtaccctt tcaggctgtc atggatcttt ccttcctcgg cggataggtc
     2521 cctgtcctcc gctatatcca tgaggtataa caggttattc tgtgcattgt acagccgctc
     2581 cagggcgctc ttggcctggt ctaaaagctc ttggctaaag tttaccgggc tcctgtaatg
     2641 agacgacagc ataaagagcc ttactatctc aaggtcgtat tccttggcga tgtcccgaac
     2701 ggtaaagaag ttaccaaggg attttgacat cttcctgttg tccacgttta tatagccgtt
     2761 gtggagccaa tagttcgcaa agggttttcc ggatagaccc tcgctctggg caatctcgtt
     2821 ttcatggtgg gggaagatga gatcctggcc cccggcatgg atatctatgg tctcccctaa
     2881 gtacttagag gccatgacgg agcactctat atgccacccg ggcctgccct ttccccaggg
     2941 gctgtcccaa gccggctctc cgggcttttg ggccttccac agggcaaagt ccatgggatt
     3001 tcgcttcttc tcgtccggat ctaccctggc cccggcttcc agctcctcta ggtcctgcct
     3061 agagagcttc ccatagcctt caaaagccga ggtgtcaaag cacacgtccc cgcctgtcac
     3121 ataggccaat cccttttcta taaggccttc tataaactct atcatttcct ctatatgctc
     3181 cgtagccttg gggtagacgt cagccctctc tatccctagg ccatctgcat ctataaagta
     3241 ttcctctata aacctctccc caagctccgc tacggtggtc ccctcttcct ccgccctctg
     3301 gatcatctta tcgtctatgt cggtgaagtt ttggacatag gtcaccttga atcccttgta
     3361 ttccatatac cttctaaagg cgtcaaagat gataaagggc cgggcgttgc ccacatgaaa
     3421 aaagttgtac accgtaggcc cgcagcaata catccttacc tcgttatcat atatgggtat
     3481 aaagttctct ttccttctcg ttagggtatt atataacttc attttgtcta acttcctcca
     3541 gttctttcag ctgcgcttcc actctggtga gcctctttac aatacagcag atagcgtctt
     3601 ccacgggatc agggagcctt acctggtcta ggtccacctg gtcgcagtcg attgtcctta
     3661 tctttggatc tctgggtacg ggaacgttgt cctttctcac tacccggccc ggtatgccta
     3721 caacggtaca gttcggtgga acctccttca gcactacggc gcccgctcct atcttgctgt
     3781 tgtctccaac ggtaaagggc cctaagacct tggcccccgt gctgatgacc actccgtttc
     3841 cgatggtggg atggcgcttg ccatgctcct tgccggttcc gcctagggtt gctccctggt
     3901 agatggtgac gttttcacct atagtggtgg tctctccgat caccactccc atgccgtggt
     3961 ctataaacag ccttttgccg atgtcggctg ccgggtgaat ctctataccc gttgtaaacc
     4021 tggcgtattg ggatatgagt ctggctaaga gttttagacg gtgattgtgg agccaatggg
     4081 ctatcttata gaagataata gcatgaaaac cgggataaca caagaaaacc tccagcgaag
     4141 ttctggcggc gggatccctg tcctttatgc cttgaatttc atctttaaac catttaaaca
     4201 aatagtacac ctcccggatg attggatacg ctacgaaaag ggtccttcat tacaaaatca
     4261 gtcaaattat ccgattttat gctaaaggga tgtcttttcg taacatatga attaagggtc
     4321 acgttttacg gcatccgtat accgatatgg gatgtttgcc gtaaaacatt taggtacttt
     4381 aaaagcgata cctaacacgc atgattataa catagtacgc aatattatta aactggttcc
     4441 gaaagcctca aacaaaaatc cctgacctaa atgcgatcag ggattatgaa aatgttttgc
     4501 ggtctgtagg gtgcggggtt tttgggcggt tagcctaaaa ggttcctaat ccaagcaaag
     4561 aatttttcta tggactctaa tatcttcttt ataacgccct tgttctcgct gaccaccttt
     4621 ttaacatcgt ctaggctgcc ggcgatcttt tctatctgcc cggctatctt ctctacgttt
     4681 aagtctaggt tactgatctt cttcatcagc ttgacgatct gctcgatctg ggcatccgtc
     4741 agattgatat taaggttggc ggatatctcg atgactatct ttcggatctc gccctcgtcc
     4801 ttggtcttta gctctgcgat cctttccttg atctccttta taagggctgc ggcctcgtcc
     4861 ttgcctatga cctcccccag ctcgccggtg gttaccagct cctcgttggc ggcctccttg
     4921 gcatcctcgt ccaattcctt gccggatacc tcttcgaagg ccttcatgat cccggtaaga
     4981 gcaccggtcc ccgatacctt gaagggagcc gcggctatga ccttggcgtt ttccacccct
     5041 gcggtcacta aggcgttggc gtacatttcc cgggtaaccc aggttatatt atgagtttcg
     5101 acatcaattc cctgaccttt atccaaaggc tttacataag ccgatgatat ggtcctgtgg
     5161 cctatcttct cgtcggatac aaggccccct aaaaactgcc tttcctcgtc tattgtgacc
     5221 gtcagtatct taacttcgtc cctttttacc ccgaacagtt ccaagatctg cttttgttgg
     5281 ctctcgttca ggtcggcgcc taggcttacg acgatttcat cgtcgggcga atcctcgccg
     5341 tccgctaagg cgtatagtgg gaatagtaga atcagcatga gggctaggcc cagagatagg
     5401 atttttttca ttttcaaaac ctccttaaaa tcgtttccaa aaactttttc accagtttgt
     5461 gttatccttt tcctttttgg aaagtccatc gattccaact tctaacttct aacctccaaa
     5521 gtcgccaccg cctttgcggc tatgccttgg cctacgccaa caaagcccag cccctcggtg
     5581 gtggtggcct ttatattgac cgcagatggg tctgccttta gaatttttgc gatgttttcc
     5641 tccatctcct ttagataagg cgccatcttg ggtctttccg ctatgattat accgtctata
     5701 ttgccgacca taaaaccatc ggctgcgatt atcccctcta ccacagagag taggtccata
     5761 ctggatatcc ccttgtattt atcatcactg tcaggaaaat gctggcctat atcccccttg
     5821 ccaagggctc ctaaaagggc atccattacg gcgtggacta agacatctgc atcggaatgg
     5881 ccctgcaagc ccttgtcaaa gggtatctca acgccgccta caataagggg gcggttatct
     5941 gccaatctat gtacgtcata tcctattcca actctcatag ctgtcctccc tgccttccta
     6001 taagaatcct cgaggctata tctatgtcct ccggagtggt tattttaata ttgtcatagc
     6061 ccccgggtat catatatacc gggctcccta aatattctgc cagtgcagaa tcatcggtgg
     6121 ctacgtagtc atccgctgca gcctttttat gggcctctat gatgagggag tatctaaagg
     6181 tctggggggt ttgaacctgc caaagggatt ctcgcctaag ggtccttctt acccttccgc
     6241 cgggttctgc ctcctttatg gtgtccttta cgggcatgcc cgctacggct gcgccgtgtt
     6301 ttacggccca ctgtacgctc tcatcgatta cctgcggggt tataaggggc ctggccccat
     6361 cgtgaatggc cactgtctcc gtgtctttag gtaattcctt taagccgtta cggacggaat
     6421 attgtctctc agaacccccg aaaactatgg ttatgggctt ttggggcggg tgtttgcgga
     6481 atatattctc tcttatgtat tcttcttccc ccggggctgt caccaatact atcctatgaa
     6541 tggagtcggc cctgtagaat gcatccagcg tatgggcgat gacgggccta tccccaaggg
     6601 gtaagtatac cttatttact tctcggttca tcctagtgcc ccttccggcg gctaggataa
     6661 tagcgtagtt tttcaatccg tcctccaact cccagcttgt aatctcaaac ttttgatcac
     6721 cggcctttaa tcccttactt ctgatctttg gtatttggtt gccgactgtt cgcttacggc
     6781 ttctggtcat tagtattcgg ttgccggctt ttgacctgta agcatctaaa ttatataaaa
     6841 ttaagcggcg agatacaacc cgccgcaaag ttttgtttga aacatttaac agttttctaa
     6901 catgtattca cagttttgtc taacttttgc cccatcaccg gtgcccggcg gaacaagtgg
     6961 gaacacagaa ggaccgtccc cttgtgttcc ttgtgttctt gtgttattac actactctgt
     7021 cgatagcttt gggcttggcg aagatcatgc gtccagccgc agtctgaagc acgctggtca
     7081 ccagaacgtc gatgctttcg cccacgtgtt ttttgccgcc atccaccact atcatggtgc
     7141 cgtcgtctaa gtaggctatg ccctggccag tctccttgcc gtccttgatc acctggacca
     7201 gcatctcttc gccaggcagg accacgggct ttagggcgtt ggccaattcg tttatattaa
     7261 gtacgggtac tccctgaaat tctgccacct tgttgaggtt atagtcgttg gttatgacca
     7321 taccctttaa gacctgcgcc agctttaaaa gcttgctgtc tacctcttga atatcgtcaa
     7381 aatccttttc gtatatgcgc acgggaatgt ccaattcctt ttgaatctga ttgagaatat
     7441 caagacccct gcggccccgg tttctcttta aggcatcgga agaatcggct atatgcctca
     7501 gttcctccaa tataaaggca gggattataa ggggaccttc tacaaacccg gtcttacaga
     7561 tatcaaatat cctaccgtca atgattacgc tggtatccaa tatctttgga tgggttgaaa
     7621 agacctcatg cccatggcgg tccttttgta ccttctttaa aagatccgac caatccagct
     7681 ccctccacct cttggtagtg acgcttatgc ccaggtaccc gaaaaacaag tagatcaaag
     7741 tcgtgagtat aagggacagc caccctattt caagttgtct aaaggggata gtcacaagat
     7801 aagaaattaa aagaccgatc ataaggccgg aaaccccaaa caaaatatcg ttagccggaa
     7861 tttcctgcag tttttgttct atcagtctaa taaagtccac ggtgaattga atcagtcttt
     7921 ttgaaaggat aaaaaataat atgccaaatg caataccaaa taagatataa ataacgatat
     7981 cctgatatcc aataagcttg atacccctat gggctaacat gggtaccgat gtccaagcca
     8041 gcgctatacc gacggctata cctaccagac caaggatgat ccttattact ctccttagca
     8101 tttgatcacc tccctcttat atta
//
LOCUS       DASRNX010000245         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_317142,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000245 DASRNX010000000
VERSION     DASRNX010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317142"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2059
                     /locus_tag="VFD89_09800"
     CDS             <1..2059
                     /locus_tag="VFD89_09800"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HZJ58511.1"
                     /translation="SNLIIALGAILFSNVYGIHVLAWATLAGGASQLAIQLPALAKKL
                     KYKPWVSLKEPTSLAYWKLVVPSIFGSAIDKINILVDNALASFLLAGSISAINYSTKL
                     VDFADNIAIGAIVAVVYPRLAGMNSKEDLGKLGNTAHKAIVGITQIVLPIIAITMIFS
                     KEIVRSVFERGAFLESDTKLTAYALSYYALGMWGIGIRGILNRVFYSLGNTSTPMKIS
                     IITLISNIVLNIAFVRYMGVGGLGLATSISCTMGVVVLFILIRKSVGTLNVGGLIREG
                     LKMLLITLGMAYGIIKYQAPQVFGNPHLDLVLKIGKGLLFYIILSWVVGAGLIREGRH
                     HKRHKNINVPGDIFLRKSLKALDKMVSRLISHVIYYARGKIDKDLLKGRISFQLAGVN
                     PSDIWIIQESIMPTNTLIIKLGVHPLEYGLICKIPLTAGAKNKLEANYNSLMELCDRN
                     HDLIPRPMGRVSIGEYEGFVETLVEGCNAYDMFLSGEADGDGLLSLSKEALIDIRANT
                     FNWGGPYPTVSSRVLFRLLADAFISYMPYNIPYDISQIFHDIHVIWNDKELRCGLAHG
                     DFWIGNIIISPGDNPRIQGVIDWDRLEHSIPVLFDEMHLEISYLGLNENQPVGHEIIK
                     CIRDRRYQFEDAMIYWLLFIIKSEEKDSRTLKSRRWLQGNVYDVLDFLERLLEWFHLF
                     TDK"
     gene            complement(2256..3455)
                     /locus_tag="VFD89_09805"
     CDS             complement(2256..3455)
                     /locus_tag="VFD89_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZJ58512.1"
                     /translation="MLNVSDRIKKTQEHLGELIKSQLTRVERMKATKEFINYGELDTI
                     IIGIVGGDGIGPYITAEAQRVLEFLLRDDAQRGKVEFKVIEGLTIENRAEEGKAIPED
                     ILDELKECHVILKGPTTTPRAGDPWPNIESANVAMRKELDLFANVRPVKVPEENIDWV
                     FYRENTEGAYALGSDGVVVNDDLFLDFTVATYEGSRRIIKAAFDYASKSGANKVTVVT
                     KANVIKTTDGKFLEIAREVAKEYPQVEWDDWYIDIMTAKLIDTNRRSQFRVMVLPNLY
                     GDILTDEAAEFQGGVGTAGSANIGTRYAMFEAIHGSAPRMVDEGRAQYADPSSIIRAA
                     GMLLEHIGYSEEAKKLAMALDICSQFEKRLVITGRENGATGGEFADYIMETLSSDDLT
                     SKWESYQ"
BASE COUNT          932 a          785 c          824 g         1021 t
ORIGIN      
        1 atcaaattta atcatagcct taggggcaat actcttctcc aatgtatacg gcatacacgt
       61 tctggcatgg gcgacgttag cgggtggggc atcccagctt gcaatacaac tgcctgcctt
      121 agccaagaaa ttaaaatata agccctgggt cagcctgaag gaacctacat ccctggctta
      181 ttggaaactg gtcgtaccca gcatattcgg atctgccatc gacaagataa atatcttggt
      241 agataacgcc ctggcatcat ttctgttggc aggcagcatc tccgccatca actattcaac
      301 aaagctcgtt gattttgcgg ataacatagc cataggggcc atcgttgcgg tggtctatcc
      361 cagactggcg gggatgaatt ctaaagagga tcttgggaaa ttggggaata ccgcccataa
      421 agccattgta ggcataacgc agattgtcct tcctatcata gctatcacta tgatcttcag
      481 caaagagata gtaaggtctg tatttgaaag gggagccttc ttagagtccg ataccaaact
      541 cacggcctat gctctgtcat actacgcctt gggaatgtgg ggcataggta tcagggggat
      601 actaaacagg gttttctatt ccttggggaa tacctctacc ccaatgaaga taagcattat
      661 aacgctgata tccaatatag tattgaatat cgcatttgta aggtatatgg gggtaggcgg
      721 attgggcctt gctacatcta tctcctgtac catgggggta gtcgtattgt ttatccttat
      781 tagaaaaagt gtgggaaccc tcaatgtagg gggcctaatt agggaaggat tgaagatgtt
      841 acttataacc cttgggatgg catacgggat tattaagtac caggcgcccc aggtttttgg
      901 caatccccac ttagatctcg tattaaaaat agggaagggt ctattatttt atattatttt
      961 atcctgggtc gtaggggccg gactcataag ggagggaagg caccataaaa ggcataaaaa
     1021 tataaacgtt ccaggcgata tttttctcag aaagtcactt aaggccctgg acaagatggt
     1081 atccaggctg atatcccatg ttatatacta tgccagggga aagatcgaca aggatctatt
     1141 aaagggcaga atatcgttcc aacttgcggg ggtgaacccc agtgatatct ggattattca
     1201 ggaaagtatt atgcccacga acaccttgat tattaaattg ggggttcacc ccttggaata
     1261 tggtctaata tgcaagatcc cattgaccgc cggtgcaaag aataaactcg aggcaaatta
     1321 taacagcctg atggaacttt gtgatagaaa ccatgatttg atccccaggc ccatgggcag
     1381 ggtatcaatc ggggagtatg agggatttgt ggagacccta gttgaagggt gcaatgcgta
     1441 tgatatgttt ctaagcgggg aagcggatgg cgatggtctt ttgagtctat ccaaggaagc
     1501 attgattgat ataagggcta atacatttaa ctggggtggc ccatatccca ccgtatccag
     1561 cagggtgtta ttcaggctgc tggctgatgc gttcatatcc tatatgccat acaatatccc
     1621 ctatgatata tcacaaatct tccacgacat acatgtcata tggaatgata aggaattgcg
     1681 gtgtggattg gcccatgggg acttttggat tgggaatatc attatctccc ctggagacaa
     1741 cccgagaatc cagggggtaa tagattggga taggttggaa catagtattc ctgttctgtt
     1801 tgacgagatg cacctagaaa tctcctatct gggcttgaat gagaatcaac cggtagggca
     1861 tgaaataata aaatgcataa gggacagaag atatcagttt gaagatgcga tgatatattg
     1921 gctgcttttt attataaaat ctgaggaaaa ggatagtagg actctaaaga gcaggagatg
     1981 gttgcagggg aacgtgtatg atgttttaga ctttttagaa cgattattgg aatggtttca
     2041 cctgttcaca gacaaataat atggggactg aatttatcgg tatgataatt acagcgattc
     2101 tgtgtagcca tatgataatc aatcctatat cccataggta gaataattta tcccaaaaaa
     2161 acatggctag gggcggcata acagccgatc ctagccgtgt ggcaatctgt atatccgtta
     2221 atttcggttc atgaaacaaa tttgctctgg cagatttatt gatatgactc ccatttgctt
     2281 gtcaaatcat cgctgcttag ggtctccatt atataatctg cgaattcgcc accggtggcg
     2341 ccgttttccc tgccggtgat aacgagccgc ttttcaaatt ggctgcatat atcaagggcc
     2401 atggccagtt ttttggcctc ttcagagtat cctatatgct caagcagcat tcctgccgct
     2461 cgtataatac tgctggggtc cgcgtattgg gcccggcctt catctaccat tctgggcgcc
     2521 gagccatgaa tggcttcaaa catcgcgtac ctggtaccga tgttcgcact cccggctgtc
     2581 cccacccctc cctgaaattc cgccgcctcg tccgttagaa tatccccata taggttgggg
     2641 aggaccataa ccctaaattg ggatctgcga ttggtgtcta taagctttgc cgtcattata
     2701 tcaatatacc aatcatccca ttccacttgg gggtactctt tggcaacctc gcgggctatc
     2761 tctaggaact taccgtctgt agtctttatt acgttggcct ttgtaacgac ggttacctta
     2821 tttgccccgg atttgctggc ataatcaaag gcagccttta taatacgacg ggatccttcg
     2881 taggtagcga cggtaaaatc gaggaacaga tcatcgttta ctactactcc atcgctgccc
     2941 agtgcataag cgccctcagt gttttcccgg taaaaaaccc agtcgatatt ttcttcgggt
     3001 accttaacgg gccgtacatt ggcgaaaaga tccaattctt tgcgcatcgc tacattggcg
     3061 ctctctatat taggccatgg atcgcccgcc cggggagtgg tggtggggcc ctttaagatt
     3121 acgtgacatt ccttaagctc gtccaatatg tcctcgggta tggccttacc ttcctcggcg
     3181 cggttttcga tggtcaatcc ttctataact ttgaattcga ccttacccct ctgggcatca
     3241 tcccttaaca ggaattctaa gaccctttgg gcttcggctg ttatgtatgg gccaattcca
     3301 tcgcccccta cgataccgat gatgatggta tccagttcac cgtaattaat gaactccttt
     3361 gttgccttca tcctttcaac ccgcgttaat tgacttttta tcaattctcc caggtgttct
     3421 tgggttttct taatccgatc tgaaacgttc aacatgcatc cctccatttt tgcattatct
     3481 ataaggtttt ttacctataa aagtattagg ttgtcccacg atgtatcata atcgccgaac
     3541 ccgaacgtat ccattatata tc
//
LOCUS       DASRNX010000246         3942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_101975,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000246 DASRNX010000000
VERSION     DASRNX010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101975"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /locus_tag="VFD89_09810"
     CDS             <1..1559
                     /locus_tag="VFD89_09810"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HZJ58513.1"
                     /translation="EYREWKANCADEHIRLMRNTVKEYGDEKAYCAEIFGMFHAHRAK
                     SSGIDLYNAKEHFDFLASPTFLSTHSASKGHYHQLSTPASSIRFMKSVNKEKQAIMLY
                     GNNGGKWRYVIEPSLESKIWLWETVSVGGGIWNCLFNGQHPGATFDNRNSYLERDVYH
                     YLADNEKILENQIPREEVGIYFSKASRDNLAEDDEDMDEYGVFIKGIENVLIDNHVQY
                     GFCPDINFTLDEVSKYKLLIIPNGAYLSDEHIEIIKQYVSMGGGLIASYETSLYDEKG
                     VRRKDFGLRDLFGCSYTGIKKDTQVDCYQMLKGPHTITSEFKNTSLLMNDGYTLLCTP
                     IDKEDYKVVCTYVPKIQNQPPPKAWIRDMRTPFSTIMAGTYGKGKVVYFANQMDKLCY
                     VNGHEDFMDIVYNSILWAKSGELSIETDAPDSVHIALTENKEDNKQAVLSLINLTSRP
                     RRPIRQLLPAYDFYVDCRLEGDSLEHYKILRSDGDINVREIKREEGMLTVRIEIGRLD
                     EFAGIYLHTK"
     gene            1739..2653
                     /locus_tag="VFD89_09815"
     CDS             1739..2653
                     /locus_tag="VFD89_09815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZJ58514.1"
                     /translation="MEYRQFGKTGAVVSRIGFGGAAAGLKNYLGKYDPEDEESRNSVY
                     AALELALERGITYYDTAPGYGGGTSERIFGDVLSKVDPEKIFLATKCFPSDDGSLGER
                     PHTRTLSDYDFVMYSVEGSLKRLRRDYVDLLQLHGNSYTKDMKDRIVGAKGALEALER
                     LKKEGIIKYIGFTTEDNNDAVYDLIRTRRFDAIQLCYNFIFQHPYEPSRPFGSLLEAE
                     KKKMGIATMRTTTSGTFQKWMRMIRPHDTFDYTRALIQFVLSNELVDVALIGMRTPHE
                     VLQNVDIVNDTSGRIDLDSLHERYVGEN"
     gene            2804..3475
                     /locus_tag="VFD89_09820"
     CDS             2804..3475
                     /locus_tag="VFD89_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZJ58515.1"
                     /translation="MNIFPQISGSIIDYDILVMGHLKWNRYFNESPEDPPRGDPSTCT
                     SVLIRGFDKCGEEYVLIIDPTLRVSPEDYHFDINRRTGLRPHEITHLFCTHHHGDHIE
                     GLNYFKNAKWCTSNEVIRDLKGTGAIDSSRLIPVEGEFLPGIYALSLPGHTDNLHGIA
                     FGHRGKKIIVASDAVMTKDHYEHNATEFQPDPEMNEVAAQTIINIKESFDLVIPGHDN
                     VIVNW"
     gene            3570..>3942
                     /gene="aac(6')"
                     /locus_tag="VFD89_09825"
     CDS             3570..>3942
                     /gene="aac(6')"
                     /locus_tag="VFD89_09825"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047663 - aminoglycoside
                     6'-N-acetyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6'-N-acetyltransferase"
                     /protein_id="HZJ58516.1"
                     /translation="MIRQAGMDDKRAAAKLAMLLWPDNEIDDLKREMLDYIVSDSGAV
                     FICFDEDTPIGFAQCSLRRDYVEGTDSSPVGYLEGVFVKENYRKRGIGGRLVAQCEEW
                     AKRQGCTEFASDCELNNSRSLE"
BASE COUNT         1202 a          770 c          987 g          983 t
ORIGIN      
        1 atgagtatag ggagtggaag gccaattgcg cagatgagca cattaggctt atgagaaaca
       61 ccgtcaaaga atatggggac gaaaaggcgt attgtgccga gatattcggg atgttccatg
      121 cccatagggc aaagtcctcg gggatagatc tatacaacgc caaggaacac tttgattttc
      181 tcgcaagccc caccttttta tccacccata gcgcatctaa gggccattac catcagttat
      241 cgactcctgc ctcaagtata cgctttatga aatccgtaaa taaggaaaag caggcgatca
      301 tgctatacgg caataacggg ggaaagtggc ggtatgttat tgaaccgagt ctggaatcca
      361 agatatggct gtgggagacc gtaagcgtag gaggcggtat ttggaattgc ctctttaacg
      421 gccaacaccc cggagcaacc tttgataaca ggaattcata tttagagagg gatgtctacc
      481 actacttggc ggataatgaa aagattttgg aaaaccaaat cccaagggag gaagtaggca
      541 tatatttttc caaggcaagc cgggacaatc ttgcagaaga cgatgaagat atggacgaat
      601 acggtgtgtt cataaagggc atagagaatg tgctcataga taaccatgtt caatatggtt
      661 tttgtcccga tataaacttt accctggatg aggtaagcaa gtataagttg cttataatac
      721 caaacggcgc atacctgtcc gacgaacata tagagataat taagcaatat gtgtccatgg
      781 gcggcggatt gatagcctcc tacgagacct ccttatatga cgaaaagggt gtgaggagga
      841 aagattttgg actcagggat ttatttggct gcagctatac cggaattaaa aaggataccc
      901 aagtagattg ttatcagatg ctaaaagggc cccataccat aacttccgag tttaaaaaca
      961 catccctgtt gatgaacgac ggatatacac tgttgtgcac gcctatagac aaggaagact
     1021 ataaggtggt ctgcacctat gttcccaaaa tccaaaacca gcccccgcca aaagcctgga
     1081 taagggatat gaggactccg ttttccacca tcatggcagg aacatacgga aagggcaagg
     1141 ttgtctattt cgccaatcag atggataagc tgtgctatgt aaatggccat gaggatttta
     1201 tggacatcgt ctacaactcc attctgtggg caaaaagcgg cgaattatcc attgaaaccg
     1261 atgcgccgga tagcgtacac atagccttaa ccgaaaataa agaggataat aaacaggcag
     1321 ttctatccct gataaatctt acctcaaggc ccagaaggcc cataaggcaa ttgctcccgg
     1381 catatgactt ttatgtagac tgcaggctgg aaggggattc cttggagcat tataagatcc
     1441 tgcgttcaga tggcgatatt aacgtccgag aaatcaaaag agaagaaggg atgctgactg
     1501 taaggataga aattggaagg ctcgatgaat ttgccggtat atatcttcat accaaataaa
     1561 tcggtaggca acctctagga cgcttttgaa caagagaaca aaaatagttc attgccggtt
     1621 aaatatgcac gaaattgcat tgcgtcagta gcatatgatt attatggtta tcaggatata
     1681 ttttttaatc ccataatcgt caatgagttt tagattatgt tatagaaggt gagaaactgt
     1741 ggaatatagg cagtttggta aaacgggggc ggtggtgagc aggattggct ttggaggcgc
     1801 tgccgccgga ctaaaaaact atcttgggaa atatgatccg gaagatgaag aatcaagaaa
     1861 tagcgtatat gcggcgctgg aactggccct ggaaaggggt attacctact acgatacggc
     1921 tcccggctat ggcgggggta cttcagagag gatctttggg gatgtgttat ccaaggtgga
     1981 ccccgaaaag atttttctgg cgaccaaatg cttcccctcc gatgacggat ctcttgggga
     2041 aagaccacat actcgtaccc tatccgacta tgattttgtc atgtactctg tggaagggag
     2101 cttaaagagg cttcgcaggg attatgttga cctcttgcag ctccacggta actcatacac
     2161 caaggatatg aaagatcgaa tagttggtgc aaagggggct ttggaggcat tagagcgctt
     2221 aaaaaaagaa ggcattataa agtacattgg ctttacgacg gaggacaaca acgatgcagt
     2281 atatgacctt ataaggaccc ggcgctttga tgctattcaa ctatgctata acttcatatt
     2341 ccagcatcca tatgagccca gcagaccctt tgggagtctg ttggaggcgg aaaagaaaaa
     2401 aatgggtatt gcaaccatga ggaccaccac ttctgggaca tttcagaagt ggatgcgtat
     2461 gataagaccc catgatacct ttgattatac ccgggccttg atccaattcg tgctgtcgaa
     2521 cgaattagtg gatgtggccc tcattggaat gcgtacaccc catgaggttt tacaaaacgt
     2581 tgatatcgta aatgatactt caggaagaat agatttggac tctttgcatg agaggtatgt
     2641 tggagaaaac taaggcctat tctgcttgaa aagcctgccg atttcgatcc ggcctctgcc
     2701 gatggaatcg gtttagggcg ttctgctttc aatagctcat taaatagata atgaactgat
     2761 actacgcgcc aatttactat taaaccgtat aggagagatg aagatgaata tatttcccca
     2821 aattagtgga tccataatcg attatgacat tctggttatg ggccacctaa aatggaatag
     2881 atactttaat gaaagtccgg aggacccacc aaggggagac ccgtctacct gtacttccgt
     2941 tttgataagg ggctttgata agtgcggaga ggagtatgtg ctgattattg atcccaccct
     3001 cagagtttct ccggaggact accactttga tataaacaga aggacaggcc ttaggcccca
     3061 tgagattacg caccttttct gtacccatca ccatggggac catatagagg gtcttaacta
     3121 ctttaaaaat gcgaagtggt gtacatccaa tgaagtgata agggatttaa aaggcactgg
     3181 ggctatcgat agttcaagac ttatccccgt cgaaggcgag tttttgccgg ggatatatgc
     3241 cctttcgctg ccgggtcata cggataatct tcatggtatt gcttttggcc acagaggcaa
     3301 aaagattatt gtagcaagtg acgccgttat gacgaaggac cattatgaac ataatgctac
     3361 agaatttcaa cccgatccgg agatgaacga agtagcggca cagaccataa ttaacataaa
     3421 ggagagcttc gatctggtca ttccgggaca tgataatgtt attgtgaatt ggtgataaca
     3481 ggaactgcat tattatgtag cgaaccccga cccccaaaaa tcccatgggc aaaggccctg
     3541 caaattcaaa actaaatgag gttataacca tgataagaca agcgggtatg gatgataaga
     3601 gggcagcagc aaaattggct atgctactat ggcctgataa tgaaattgac gatttgaaaa
     3661 gggaaatgct agactatatt gtctcagaca gcggagcggt ttttatctgc ttcgatgagg
     3721 atacgcccat aggctttgca caatgcagcc tgagaaggga ttatgttgag gggacggaca
     3781 gcagccctgt cggatatctg gaaggcgttt ttgtaaaaga gaattatcgt aaaaggggta
     3841 tcggagggcg attggtggcc caatgtgaag aatgggcgaa aaggcaaggc tgtaccgaat
     3901 ttgcaagcga ctgtgaactg aacaatagcc gaagtctaga gt
//
LOCUS       DASRNX010000247        21109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_317840,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000247 DASRNX010000000
VERSION     DASRNX010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 21109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21109
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317840"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      42..220
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            380..1390
                     /locus_tag="VFD89_09830"
     CDS             380..1390
                     /locus_tag="VFD89_09830"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZJ58517.1"
                     /translation="MKKFNLAIVGVTGMVGQKFLEVLQERDLPVDKYFMFASARSAGK
                     TVSLMGNEYTIEELTPSCFDDKEIDIALFSAGGNISKTYAPIAAEKGIVVIDNSSAWR
                     MEKDVPLVVPEVNPEEVLNHKGIIANPNCSTIQAVVALKPLHDRYGIERIIYSTYQSV
                     SGAGVEGYDDLKNGIEGKEPRKFPHPIFDNCIPHIDSFLDNGYTKEEMKMIEETKKIL
                     KDDSLKITATTVRVPVYNSHSESINVELKKDFELDELKDVLSMAPGIEVMDDPKNNVY
                     PMPANSNGKDQVFIGRIRRDESLDHGVNLWVVADNIRKGAATNTVQIAEKLMEYWSNN
                     DL"
     gene            1420..2175
                     /gene="dapB"
                     /locus_tag="VFD89_09835"
                     /pseudo
     CDS             1420..2175
                     /gene="dapB"
                     /locus_tag="VFD89_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
     gene            complement(2918..4177)
                     /gene="purD"
                     /locus_tag="VFD89_09840"
     CDS             complement(2918..4177)
                     /gene="purD"
                     /locus_tag="VFD89_09840"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZJ58518.1"
                     /translation="MKVLVVGGGGREHAIVWKLSQSPRISDLYCAPGNGGIGGIARCI
                     DIEATDIESMVQFARDQSIDLVVVTPDDPLALGMVDAMAEAGIKAFGPNKNAAIIESS
                     KVFAKDLMKKYDIPTGEYRVFSCYNEALDYIRESEFPLVVKADGLALGKGVIIAEHFD
                     EARDAIREIMIEKVFGGAGNQVVIEEFLMGQEVSLLAFSDGHTTVPMVSSQDHKRAFD
                     KDEGPNTGGMGAFSPSRVYTPEIAHIVETTMLQPTIDAMNEEGRPFKGVLFLGLILTE
                     DGPKLLEYNARFGDPETQVVLPRLRTDLLDILLAVVDERLDEIDIEWEDNAAACVVMA
                     SGGYPGSYDKGYPIHGLQNLDDREGVMVFHAGTRGDRDQYFTHGGRVLGITSVAGDLD
                     KAIQKAYKSVDLIKFKDMHYRRDIGIK"
     gene            complement(4230..5780)
                     /gene="purH"
                     /locus_tag="VFD89_09845"
     CDS             complement(4230..5780)
                     /gene="purH"
                     /locus_tag="VFD89_09845"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZJ58519.1"
                     /translation="MKKKRAVISVSDKTGIVEFAERLNDMGIEIISTGGTSKALGEAG
                     IPVTNISDVTGFPECLDGRVKTLHPNIHAGILAVRDNPHHMDQLGNLGINTIDFVVIN
                     LYPFKETILKEGIELKDAIENIDIGGPTLLRAAAKNYQDVAVIVDPKDYALIIAELEE
                     KNHISSKTRFRLAGKVFEHTAHYDSLIAKYLGEQIGEEKFPNLLTLTFEKAQSMRYGE
                     NPHQGAVFYKEIGKRPGSLINAVQLHGKELSYNNINDTNGALELLKEFEVPTVVAVKH
                     ANPCGVGSGEGIFEAYDKAYRADPVSIFGGIVVANDRVDEKTAEAINKIFVEIVVAPS
                     YTVKALEILKQKKNIRILELKDIRAGVPEGTYDMKKVYGGLLVQEANTRLLPDLSDIE
                     IVTKKSPSAKEMEDLIFAWKLVKHTKSNGIAIAKDSQSLGIGPGQVNRIWAVNQAIER
                     SGTDVKGAVLASDAFFPFPDCVEAAAAAGITAIIQPGGSIRDQDSIDECDKHGIAMVF
                     TGMRHFKH"
     gene            complement(5830..6453)
                     /gene="purN"
                     /locus_tag="VFD89_09850"
     CDS             complement(5830..6453)
                     /gene="purN"
                     /locus_tag="VFD89_09850"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZJ58520.1"
                     /translation="MKNIGVFISGGGSNLQTIIEAIDNGDIAARISLVISSRKGVYGL
                     DRAKEHGIPYAVVAKGDYKDPDSFSKAILELLEEKRVDLVVLAGYLNILPKKVVERYK
                     NRIINIHPSLIPAFCGRGYYGKRVHQAVLDYGARVTGATVHLVDEGTDTGPIILQQPV
                     EVLPEDDADSLAKRVLKVEHQLLPKALRLLTEDRISVDGRRVIIKNT"
     gene            complement(6453..7478)
                     /gene="purM"
                     /locus_tag="VFD89_09855"
     CDS             complement(6453..7478)
                     /gene="purM"
                     /locus_tag="VFD89_09855"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZJ58521.1"
                     /translation="MRWTYKDAGVDVESGYKAVDLMKKHILKTHTPQVMGGVGSFAGL
                     YALDTKNIKEPVLVSGTDGVGTKLQLAFLLDKHDTIGIDCVAMCVNDIVCQGAAPMFF
                     LDYLATGKLEPEKAAAVVEGIAEGCIESGCALIGGETAEMPGFYKEDEYDLAGFAVGI
                     ADRDRIIDGSQIEDGDVIIGLESSGTHSNGYSLIRKLVIEEEYRLEDHDEALGITVGE
                     ELLKPTRIYVKAILRALSSYTIKGIAHITGGGFIENIPRMLPKGLGAVIEEGTWPVKP
                     IFELISGWGNIRKEDMFNTFNMGIGMAVACKATDSDGLVKLFIDEGYPAHIIGRVKKD
                     LEGVVFV"
     gene            complement(7484..8917)
                     /gene="purF"
                     /locus_tag="VFD89_09860"
     CDS             complement(7484..8917)
                     /gene="purF"
                     /locus_tag="VFD89_09860"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZJ58522.1"
                     /translation="MYSKEIYRDDKLKEACGVFGVLDVDDLKVEELTYYGLYSLQHRG
                     QESAGIAISNGKNIKYHKAMGLVAEAMDNKSLSRLRGGEIAIGHVWYSTDGDVSVHNA
                     LPLVMKYKKGTMAIAHNGNLVNADDLRKSLEDQGAIFQTTTDSEVIANLLSRQNADTF
                     EFGVLDTLGTVKGSYALVIMTEDKLIAARDPHGMRPLALGKLENSYVIASESCAFDII
                     GADFIRDVRPGEVITISRRGLESMQIPTPLKTSLCIFEFVYFARTDSNIDGISVYMAR
                     KEAGRRLAEEQPVDADLVIGVPDSGTTAALGYSEASGIPFGEGLVKNRYVGRTFINPD
                     YNMREQNVRIKLNALTRIVRGKRVVMVDDSIVRGTTSKKIVEMLRLAGAKEVHMRISS
                     PPVKHSCHFGVDTPGTKHLIGASYTIEEIKQIIGADSLGYLSTEGLLKTVEESGCGFC
                     LGCFSHDYPVELDWNMGKDSTDTRTEG"
     gene            complement(8957..9457)
                     /gene="purE"
                     /locus_tag="VFD89_09865"
     CDS             complement(8957..9457)
                     /gene="purE"
                     /locus_tag="VFD89_09865"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZJ58523.1"
                     /translation="MSGGYKVAFIMGSNSDFPIVKPGIELLKSFGVGVEVKILSAHRT
                     PDHASEFAKTAKERGIGVIIGAAGKAAHLPGVMAAYTTLPVIGLPIKSSTLDGLDSLL
                     SIVQMPRGIPVATVAINGSENAALLAVQILALGDTKLAEVLKQYRVSMEDEVLAQNDS
                     IQEDLN"
     gene            complement(9617..10972)
                     /locus_tag="VFD89_09870"
     CDS             complement(9617..10972)
                     /locus_tag="VFD89_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HZJ58524.1"
                     /translation="MSGFLDRTFKLREHKTDVKTEIMAGITTFMTMAYILIVNPNILS
                     GAEIGIELMDRGAVFTATALSAAIATIAMAFLANYPVALASGMGLNAFFAFVVAVKYS
                     WQIALTAVLIEGIIFIIMSFFKFREAIVNGIPENLKYSVTVGIGLFIAFIGLQNAGII
                     TADGAVLVGLGDLKNITSILFFVGLIGTIYMNTKGVKGALLWGILGTYALGIICQLTG
                     LYTVDIEAGRFNLIPQEGIISLPPSIASTFLKFDFQGSIKLGFEFIVILFSFLFVDIF
                     DTVGTLIGVASKGNLLDKDGKLPKAKEALLADAIGTVAGACLGTSTVTSYVESASGVA
                     EGGRTGLTALVSGLMFLVALVFSPVLTIIPSFATAPILVTVGLFMVTAIAKVDFSDYT
                     EALPAFLTIIMMPLAYSIADGIMFGMLSWVILKLVTGKAKQINPLMYILALLFVLKII
                     L"
     regulatory      complement(11098..11197)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(11412..12128)
                     /locus_tag="VFD89_09875"
     CDS             complement(11412..12128)
                     /locus_tag="VFD89_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZJ58525.1"
                     /translation="MNFIKSEIKKWSRDPMMGFMVIYPIFFAMMGRYLLPWIGDRYGF
                     DFIPYTDLILVIMVLIIPISYGALIGFSILEDRDDNIIINIRATPLSIHKFLSFRLVM
                     VFFLCFSATLFTIVVSNIVDFSPKNVIAVAFLASLEAPMAGLLINSFSKNKIEGFAVM
                     KAGGSIIIFPIVALFFSDIRELFFSFAPGFWTGKVISCLIRGNIFYLSCNQYYIIGLG
                     YMLLLNVLVYRLFIRRVILN"
     gene            complement(12136..12861)
                     /locus_tag="VFD89_09880"
     CDS             complement(12136..12861)
                     /locus_tag="VFD89_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58526.1"
                     /translation="MVRFFALIKGELLRLARYKLFAASIFVSMMWVGVLHFVDMENVT
                     GLIPQLVFMDVTTMAVLLVGVTFIYERDEATLYSMLISPITKSEYILAKLVSHTTPGI
                     VSLSIMYIYALAFKTVDINYFLLLGGTVLVAFFHSQIGFLLTYYSRDFTDLVMYIIAY
                     FLVSILPVILDEFAIITNAVFKKLVFLIPTKAALMIIVGTTGVIESWKIALAVTYIII
                     GSVLLHYLIWRKLHRHFLRGGGE"
     gene            complement(12854..13717)
                     /locus_tag="VFD89_09885"
     CDS             complement(12854..13717)
                     /locus_tag="VFD89_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58527.1"
                     /translation="MTKLYHIRDLRFRYPKNSEDTIRGISFDIRRGEIFGLLGPSGVG
                     KSTTQKLLIKLLGNYRGEILYEGKDLHSYRDSYFQDIGVGFEVPVHFSKLTAEENLRF
                     FKQLYASGMDTDTLLKRLGLYEDRNRRVSQYSKGMKVRLNFVRAMLNNPNVLFLDEPT
                     NGLDPKNARVIKEIIKEFRQGGGTVLLTTHLMNDVDELCDRVAFMADGKIVEISTPRE
                     LKLKYSNRELDVEYIDDGSVTKSTFSLDNLGSNNEFVDIIDKRKILTLHSRESTLDDI
                     FIKVTGVDTNG"
     gene            complement(13956..15215)
                     /gene="guaA"
                     /locus_tag="VFD89_09890"
     CDS             complement(13956..15215)
                     /gene="guaA"
                     /locus_tag="VFD89_09890"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZJ58528.1"
                     /translation="MKHEMVLVLDFGGSHSQDIARKVRESRVYCEVLHSDVSVERIRD
                     IAPKGIIISARGDDAGQLDSQKRFGQIYDIGVPVLVIDSWNDLDQLIDFLFEECGCWG
                     TWDMATFIEISIDNIKEKVGDKRVLCALSGGVDSSVAAVLMHKAIGEQLVCIFVDHGL
                     LRKNEGDEVEKIFKDQFHLNLIRVNAQDRFLDRLAGVSDPEIKRKIIGEEFIRIFEEE
                     SKKLGEIDFLVQGTIYPDVIESGGGESDLVKSHHNVGGIPEDVDFKEIIEPLRDLFKD
                     EVRRVGEELDIPGHMVWRQPFPGPGLAIRIIGDVTREKLEILKEADAIYRDEISIAGL
                     DREIWQYFAVLTDMKSVGVTEGKRTYDYTIALRGVTSIDGMRADWARIPHDVLDKIST
                     RITNEVPLVNRVVYDISSKPPATIEWE"
     gene            complement(15255..16712)
                     /gene="guaB"
                     /locus_tag="VFD89_09895"
     CDS             complement(15255..16712)
                     /gene="guaB"
                     /locus_tag="VFD89_09895"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZJ58529.1"
                     /translation="MNNKFVKEGLTFDDVLLIPGRSEVLPGQVDLTTNLTKSIKLNIP
                     LMSAAMDTVTEARLAIAIAREGGIGIIHKNMSIEKQAIQVDKVKRSEHGVIVDPFFLS
                     PEHLLSDASDLMRKYRISGVPITIKGKLVGIITNRDIRFETDFTQRISDVMTSKNLIT
                     APQGTNLQQAQEILRKHKIEKLPIVDDDMMLKGLITIKDIEKAIQYPNSAKDSGGRLL
                     VGAAVGVSTDTVDRVTALVDAKVDVIAIDTAHGHSVGVLKTVRRIKEEFPSLQIIAGN
                     VATGEATRDLIEAGADCIKVGIGPGSICTTRVVAGIGVPQITAIHDCAEVADGYGIPI
                     IADGGIKFSGDIPKAIAAGASVVMVGSLLAGTEESPGEMEIYQGRSFKVYRGMGSMGA
                     MAAGSKDRYFQEDAQKLVPEGVEGRVPFKGSLSDTAFQLIGGLRAGMGYCGTPTIEAL
                     RRDSRFIRISNASLRENHPHDIYITKEAPNYSIGG"
     gene            complement(17032..17907)
                     /locus_tag="VFD89_09900"
     CDS             complement(17032..17907)
                     /locus_tag="VFD89_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HZJ58530.1"
                     /translation="MKIQEDKNKGILRIEGLSNFNPRHIFECGQAFRWNWDGEGYVGV
                     ASGRVLRMLWDGATLTLENTTLKDFNAIWRDYFDLDTDYGEIIKELSKDSILQEAIKH
                     GWGIRILNQDPWETLISFIISARNGIDRIKTTVEKLSQRFGIRIPQEGGDHYRFPDVD
                     ALADADESELRGCGCGYRGRYIKGTARMIRDGEINLGALKTMDYENAKEQLLKCPGVG
                     PKVADCILLFSLRKGQAFPVDVWIGRIMEELYSRDMVDFKDIRGFADNRFGNLAGYAQ
                     QYLFYYAREKQIGKK"
     gene            complement(17956..19359)
                     /gene="gltA"
                     /locus_tag="VFD89_09905"
     CDS             complement(17956..19359)
                     /gene="gltA"
                     /locus_tag="VFD89_09905"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HZJ58531.1"
                     /translation="MSLVLTRVPVGEQEPEVRKHNFNEVSLGYTLEEAIQEAKRCIQC
                     KRPRCVDGCPVNIRIPEFIDHIANGNIDRAAEIIKETNNLPAICGRVCPQEEQCEVKC
                     ILGIKGEPVAIGRLERFAADYALEKAKKGPVPMGNGIKVAIVGSGPAGLTAAADLAKY
                     GYEAHIFEAFHEPGGVLIYGIPEFRLPESLVRKEIEGIKDLGVQIYTNIIIGKTITID
                     ELLEQGYEAVFIGSGAGLPRFMNIPGENLNGVYSANEYLTRINLMKAYDFPNNHTPIK
                     VGHRVAVVGGGNVAMDAARSALRLGADEVRIVYRRSEEEMPARSEEIHHAKEEGIVFD
                     TLANPIEIMGDNGWVTGLKCIRMELGEPDSSGRRRPMKIQGSEFIQDVDIVIMAIGTS
                     PNPLIVSTTDDLAAHGWGGIIVDEETGSTSKDGVYAGGDAVTGAATVILAMGAGKKAA
                     KAIHERLKAREDLLLPL"
     gene            complement(19455..20285)
                     /locus_tag="VFD89_09910"
     CDS             complement(19455..20285)
                     /locus_tag="VFD89_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HZJ58532.1"
                     /translation="MFEIMDKRTIGPGIILMKVKAPLVAGKAMPGQFIMLRVSEEGER
                     IPLTIADYDREEGSISIIFQEVGYTTRDLGMLGVGDSIRDFAGPLGKASKLDNHGRVL
                     CVGGGVGIAPLFPQIKYLNSRGTDVDVIIGARNSDCLILVDEIKACSSNVYVATDDGS
                     SGHEGFVTDVVKRLIDEGNRYDLVIAVGPLIMMKIVSSITKEYNIPTTVSLNPIMVDG
                     TGMCGGCRVTVGGKVFYACVDGPDFDGHLVDFDEAIRRQQMYKEEEKVEHSCRLLPNE
                     "
     gene            complement(20375..>21109)
                     /locus_tag="VFD89_09915"
     CDS             complement(20375..>21109)
                     /locus_tag="VFD89_09915"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZJ58533.1"
                     /translation="IIDDGLLIKGSKVIAGISAKREATMVASVRRALFMDRVHASGVK
                     ESIDIAGPDAILILGTSVNMIHRIVNNLRLPPAEEIIKIEDISTEEEIQAARTIRREQ
                     GKHVIPVPALEIRQDFSGYLLHPLRILRFTERGRKIETLEKTVMRPTYSYMGKFFIAD
                     LALESIASHNAYKVEGVYRVTKATSISHAGGVVINLEMTVVYGYKIHEIIGKIQGNIS
                     NDIVHLTGINVLQVNVTAKSIVIENS"
BASE COUNT         5508 a         5436 c         4230 g         5935 t
ORIGIN      
        1 gaatttgggc taatagccga aaagaatttg aaaataacat aattgcagtt tagaggcgca
       61 tcttttataa gtaagttacc tgagaatgcg gaattcttgg aaggagccca aaggagaaga
      121 tgccgaaagg tataagacac cgcattttat atcttggtcc ccggattaac agtccccgga
      181 ttgtcactga gcccgtgttt ggtggagagc taactgtttt cgaaatgttt gctgagatag
      241 cattgacaaa atatacgaaa gcggctggtg atgcatcagc cgctttccat ttgggagcaa
      301 tacatcctcc agccggttgt ttttttgaga atgtctgttt taaaatatta taagtataat
      361 tattctgagg aggcaaatca tgaaaaagtt taatcttgcc atagtcggtg ttacaggtat
      421 ggtgggccag aagtttctgg aggtactaca agagcgagac ctgccggttg ataagtattt
      481 tatgttcgca tcagcccgtt ctgcaggtaa aaccgtctcg cttatgggta atgaatatac
      541 catagaggag cttacaccct cttgttttga tgataaggaa attgatattg cgctgttttc
      601 ggcagggggc aatattagca agacctacgc ccccatagcc gcagaaaaag gcatagtggt
      661 tatagacaac agcagcgctt ggaggatgga gaaggatgtc cccctggtgg ttcccgaagt
      721 gaaccccgaa gaggtcttaa atcataaggg tattatcgcc aatccaaact gctctaccat
      781 ccaggctgta gtagccctaa aacccctcca tgatcgctac ggcattgaaa ggatcattta
      841 ttccacttat caatccgttt cgggggctgg ggttgaagga tacgatgacc ttaaaaacgg
      901 catagaggga aaagagccca gaaaattccc tcatccgatc ttcgataact gcatccccca
      961 catagattct tttttggata acggatatac caaagaggaa atgaagatga tcgaagagac
     1021 caaaaagatc ctaaaagatg atagcctaaa gattaccgct acgacggtaa gggtgccggt
     1081 ctataacagc catagcgaat ctataaacgt agagctcaag aaggattttg aactggatga
     1141 attgaaggat gtactatcca tggctcccgg gatagaggtt atggacgatc ctaaaaacaa
     1201 tgtttatccc atgcctgcca attcaaacgg taaggatcag gtctttatag gcaggatccg
     1261 gagggatgag agtctggacc atggagttaa tttgtgggtt gtcgcagata atatcagaaa
     1321 aggggccgct accaatacgg ttcagattgc tgaaaaatta atggaatatt ggagtaataa
     1381 cgacctttaa attatacgcg gataggagtg tccttgcata tgataaacgt aatcatcaac
     1441 ggatgtaacg gcaaaatggg tcaggtgatt gctgctatgg cagctgccca atccgatata
     1501 agaattgttg ctggtgtaga cagatacccg gattcctttc ataatccgtt ccctgtctat
     1561 ccttcctttg acgaatgtcc ggtggaatgc gatgttgttg tagatttttc attgccccaa
     1621 gccttacctt ccctgctaaa atggggttta aacaaaaaat gtgctctggt tattgccacc
     1681 accggtttta atgaaaggga gcagcagacc atagaggaat atagcaaaga gatccccatc
     1741 tttcaggccg ctaatatgtc cataggaatt aacctaatgt atcaattgat ccagcaggct
     1801 gcccaggttc ttggggactc ctacgatatt gaaattatag aaaaacacca taatgaaaag
     1861 gttgatgctc ccagcggaac tgcctatgca ctggccaatg ccattaacga ggtgttttta
     1921 aattccaagc actatatcta tggaagacat accaaaaacg atatgagatc accctctgat
     1981 atgggtatcc atgccatccg cggcggcacc ctagtaggcc agcatgcggt catgttcgca
     2041 ggagaagacg aggtaatcga aattgaacat accgcatact ccaagcagat attcgctgtg
     2101 ggggctctta gggcagccaa gtatatggcc cataaaggtc ccggactgta taatatgaag
     2161 aatgtactgg acgaaagaag tccggttacc agcatcacca cccagtccga tattatcttg
     2221 gtatccatac gaaatgcgcc tgccagactt aacaccattg ccaatatatt caaagagttt
     2281 gcaggggaaa atgaatattt ggagctgatc agtcaaattt cacctgcaga caggatgacc
     2341 aatataacct ttgcgctttc tgcaaaggat cttgagaaag cggaggcgtt ctgtcacaac
     2401 ctagccgcca aggataagga tctggtaatc aagatggata ccaatatcat aaagataacc
     2461 atagaaggcc tgggccttga acagcaaccc cgggtggctg ccaccgtctt cgaattaatc
     2521 aacagccata atatccccat caaggcttcg gttacttcca acctcaagat cgactgcttc
     2581 atagacaaga tctacgaaaa gctgaccatg gacaccctaa tcgaaggttt cggtctctga
     2641 taataaattc gtttttcgcc agacgatata tttttttaac atatcctata ccaaatacag
     2701 gctaaaacta aaggtctctg aaaatattca gagacctttt atagataaac aatagataga
     2761 ctagatttgt cggcatgaaa atcgcggtgg ttctacacaa cttcgcggtg ataaactttt
     2821 ttagttggag gtgtttatct atattatatc cccctaaaga tatagggcat gccgacttta
     2881 attctattcg catccggata atataccatg gataggtcta ctttataccg atatccctgc
     2941 ggtaatgcat atccttaaat ttgattaaat ccacgctttt ataagccttt tgaattgcct
     3001 tgtctaaatc gcccgctact gatgttatcc ctaaaacccg gcccccatgg gtaaaatatt
     3061 gatccctatc cccgcgggtt cccgcatgga acaccataac cccttcgcga tcatccaggt
     3121 tttgcagtcc atggatggga tagcccttat cataggaacc gggatagccg ccggatgcca
     3181 tcactacaca tgctgcggcg ttgtcctccc actcaatatc aatttcatca agcctctcgt
     3241 ccaccacggc cagaaggatg tccaacagat ccgtcctcag cctgggcaga acaacctggg
     3301 tctccgggtc accgaacctt gcattgtatt ctaagagctt cggaccgtcc tcagtcagaa
     3361 tcaaccccaa aaacaggacc cctttaaagg gacgcccttc ctcgttcatc gcgtctatag
     3421 tgggctggag catcgtggtc tcaactatat gggcaatttc aggggtatat accctgctgg
     3481 gggaaaatgc gcccatgccc ccggtgtttg gcccctcgtc cttatcaaat gctcttttat
     3541 ggtcctggga gctgaccatg ggtacggtgg tgtgcccatc gctgaaagct aacagggaga
     3601 cctcctgtcc catcaaaaac tcttctatta caacttgatt tccggcacca ccaaaaactt
     3661 tttctatcat tatttcacga atagcatcac gggcctcatc aaaatgttcg gcgatgataa
     3721 cccctttccc cagggccagg ccatcagcct ttaccaccaa aggaaattcg gattctctta
     3781 tataatccaa ggcttcattg taacatgaga ataccctata ttcccccgtg ggaatatcat
     3841 actttttcat aaggtccttg gcaaatacct tgctgctctc aattatggcc gcattcttat
     3901 tagggccaaa ggccttaatc ccggcttcag ccatagcgtc caccatacca agggccaggg
     3961 gatcgtcagg agttacaact accagatcga tggactgatc cctcgcgaac tgtaccatac
     4021 tttctatatc cgtagcttct atatctatgc agcgggctat tccccctatc ccgccgttgc
     4081 cgggagcgca atataaatcc gaaatcctgg gactttggct gagcttccat acgatggcat
     4141 gctcccttcc tccccctcct acaactaaaa ccttcatcat gtcacttcct tattcttgcc
     4201 tattatttcg gttgcgtgag atctctgcct tagtgtttaa aatgccgcat ccccgtaaag
     4261 accatggcaa ttccatgttt atcgcattcg tctatggaat cttgatccct gatcgaaccc
     4321 ccgggctgta ttatagcagt gattccggca gcggctgcgg cctccacaca atcaggaaac
     4381 ggaaaaaatg cgtcggatgc aaggactgca ccttttacgt cggtgccgct tcgttctatg
     4441 gcttgattga ccgcccaaat cctatttacc tgcccaggac cgatccctaa ggattggctg
     4501 tcctttgcta tggcgatacc gttggatttg gtgtgcttga ccagcttcca tgcgaagata
     4561 aggtcctcca tttccttggc tgacgggctc tttttcgtca ctatttcaat atccgacagg
     4621 tcgggaagaa gcctggtatt tgcttcctgg accaacaatc cgccgtatac ctttttcata
     4681 tcgtatgtac cctcaggtac tcctgccctt atatccttta gttccagtat ccttatattc
     4741 ttcttttgct ttaggatctc caaagccttt acggtatagg atggggccac gacgatttca
     4801 acgaagatct tgttaatggc ttcggccgtc ttttcgtcca ccctatcgtt ggcgacgacg
     4861 atgccgccga agatggaaac gggatctgcc ctatacgcct tatcataggc ttcaaagatg
     4921 ccttcgccgc tgcccacccc acaggggttg gcatgcttga ctgccacaac cgtgggcacc
     4981 tcaaattcct tcagaagttc caaggccccg tttgtatcgt taatattgtt ataggagagt
     5041 tccttaccgt gaagctgcac cgcatttatt aggcttcccg gcctctttcc tatctctttg
     5101 taaaagactg caccttggtg gggattttca ccgtagcgca tggattgtgc cttctcaaag
     5161 gtcagggtca acagattcgg aaacttttct tcgccgattt gttcccctag atatttcgct
     5221 ataagtgaat cataatgagc cgtatgctca aaaaccttac cggctaatcg aaatctggtc
     5281 ttggatgata tatgattctt ttcctcaagt tcggctatta tcaaggcata gtccttcggg
     5341 tccactatta ccgccacgtc ctgatagttt tttgctgccg ccctaaggag ggtgggacct
     5401 ccgatatcaa tattttctat ggcatctttt agctctatcc cctcctttag gattgtttcc
     5461 ttaaagggat agaggttaat cacaacgaaa tcaattgtgt tgatgcccag gtttcccagc
     5521 tgatccatgt gatggggatt atcccttaca gccaaaatcc cagcatggat atttggatgg
     5581 agggttttta ccctgccgtc taagcattcg ggaaatcccg ttacatcgct tatattggtg
     5641 acgggtatac ctgcttcgcc taaggccttt gaagtaccgc ctgttgatat tatttcgatg
     5701 cccatatcgt ttagtctctc tgcaaactca acaattcccg ttttatcgga cacgctgatg
     5761 acagcgcgct tctttttcat cttttcttcc cccaattcta caaaaatcgg aatactaaat
     5821 cttaatcgcc taagtgtttt ttattataac cctgcgcccg tccaccgaga ttctatcctc
     5881 ggtcaataac ctaagggctt tgggcagcag ttgatgctct acctttaaca cccttttcgc
     5941 taaggaatcg gcatcatcct ccggcagaac ctcgacgggc tgctggagga ttatcggccc
     6001 cgtatcggtt ccctcgtcta ccagatgaac cgttgccccc gtgacccttg ctccataatc
     6061 cagtacagcc tggtgcaccc ttttgccata gtatcccctg ccgcaaaagg ccgggattaa
     6121 ggacgggtgg atattgataa tcctattctt atacctttcc accactttct tagggaggat
     6181 attcaggtat cccgccaaga ccaccaaatc cacccttttc tcctccagca gctccaagat
     6241 ggccttgctg aaggaatccg gatccttata atcccctttg gcgactacgg catatggaat
     6301 gccatgctcc tttgccctat ccaaaccata gacacctttg cggctggaaa taacgaggct
     6361 tatgcgggcg gctatatccc cattgtcgat ggcttctata atggtctgaa ggttagatcc
     6421 ccctccagaa atgaaaaccc cgatgttctt catcagacaa agacaacccc ttccaggtcc
     6481 tttttgaccc ttcctataat atgggcagga tacccctcat ctataaaaag ctttaccaag
     6541 ccgtctgaat ccgtcgcctt gcatgccacc gccataccga tccccatgtt gaaggtattg
     6601 aacatatcct ccttacggat attaccccag ccgcttatca gctcaaatat aggctttact
     6661 ggccatgttc cttcctctat gacggctccc agccctttag gtaacatcct tggaatattt
     6721 tctataaatc ccccgccggt aatatgggct atacccttta tggtatagct gcttaatgcc
     6781 cttaatatgg cctttacata gatcctggta ggttttagaa gctcttcccc aacggtaata
     6841 ccaagggctt cgtcgtgatc ctctaagcga tactcttcct ctattaccaa ttttctaatg
     6901 agggagtaac cgttgctatg ggtcccggat gattcaaggc caatgatcac gtccccgtcc
     6961 tctatttgag aaccgtctat tatcctgtca cgatccgcta ttcctacggc aaaccccgcc
     7021 aaatcatatt catcttcctt gtagaatccg ggcatttcgg cggtctctcc gcctattagg
     7081 gcgcatccgg attctatgca gccctcggcg atcccttcga caacggcggc tgccttctca
     7141 ggttccagct tccccgtcgc taaatagtcc aggaaaaaca taggcgcagc tccctgacag
     7201 acgatatcgt taacgcacat ggccacgcag tcaatgccta tggtgtcgtg tttatcaagc
     7261 aggaaggcca gctgtaattt tgtacccact ccgtcggtcc cggataccag aaccggttcc
     7321 ttgatgtttt tcgtgtccag cgcataaagg cctgcaaaac ttcccacgcc ccccatgacc
     7381 tgcggcgtat gggtctttag aatatgcttc ttcattagat caacagcctt ataccccgac
     7441 tctacatcaa cacccgcatc cttataagtc cacctcatcg tacttaaccc tccgtccttg
     7501 tatccgtact gtcttttccc atattccaat ccagctccac cggataatca tggctgaagc
     7561 atcccaagca aaaaccgcaa ccgctctctt caacggtctt gaggaggccc tccgtactca
     7621 ggtatccaag agaatccgcc cctattatct gtttaatttc ttctatggtg tatgacgccc
     7681 ctatgagatg cttagttccg ggagtatcaa ccccaaaatg gcaagaatgt tttacaggcg
     7741 gcgaacttat tcgcatatga acttctttgg ctcccgccag gcgcagcatc tcgactatct
     7801 tcttactggt ggttcccctg actatggagt cgtccaccat aacaaccctc tttcccctga
     7861 cgatccgggt taaggcattg agttttattc gtacattttg ctctctcata ttatagtcgg
     7921 ggtttataaa ggtcctcccc acgtatcgat ttttcaccaa cccctcaccg aagggtatgc
     7981 ccgaggcttc cgaatagccc agggctgccg tcgtgccgga atccgggaca cctattacca
     8041 ggtccgcatc gacaggctgt tcttcggcca gcctgcggcc cgcttccttt ctcgccatat
     8101 atacgctgat tccgtcgata ttgctgtcgg tgcgggcaaa gtatacgaat tcaaaaatgc
     8161 atagagatgt cttcagcggg gtgggaatct gcatgctctc aagacctctg cggctgattg
     8221 taattacttc accaggcctc acatccctga taaaatctgc cccgatgatg tcaaaggcac
     8281 agctttccga ggctataacg tatgagtttt ccagctttcc aagggccaga ggtctcatcc
     8341 cgtggggatc cctggcagct atcagcttat cttccgtcat gataaccaag gcgtaggaac
     8401 ccttgacggt ccccagtgta tctaaaaccc cgaattcgaa ggtatcagca ttttgacggg
     8461 ataaaaggtt ggcaatcacc tcgctatccg tagtagtttg aaagattgct ccctgatcct
     8521 caagggattt tcgaagatca tccgcgttga cgaggttacc attatgggca atagccatgg
     8581 ttcccttttt gtatttcatg acgaggggca gagcattgtg gacacttaca tctccgtccg
     8641 tcgaatacca tacatggccg attgcaattt ccccgccccg caatcgggac agagatttat
     8701 tgtccatagc ctccgctacc agacccatag ctttatgata tttaatattc ttcccgttgg
     8761 atatggcaat tccggcgctc tcttgccccc tatgctggag ggaatatagg ccgtaatagg
     8821 tcagctcctc tacctttaaa tcgtcaacat ctaaaactcc aaaaactccg caggcttcct
     8881 ttaatttatc gtcacggtat atctctttac tatacatttt ttatgtcttt acctccgcct
     8941 cttaatggac tgtatgtcaa ttgagatcct cttgtatgga atcattttgt gctaatacct
     9001 catcttccat agagacacga tattgtttta gaacctctgc taatttcgta tcacctaaag
     9061 ccaggatttg gaccgccaac aatgccgcat tttcgcttcc gtttatggca actgtagcca
     9121 ccgggatccc tcttggcatc tgtacaatgg acagtaggga gtccaagcca tctaaagtgg
     9181 aagacttaat gggaaggcct atcaccggaa gcgtagtata agcagccatc acccccggca
     9241 ggtgagcagc ctttcccgca gctccaataa tcacaccgat tcccctctcc ttcgctgttt
     9301 tagcgaattc tgaggcatgg tcgggggttc tatgggctga taggatcttg acctcgaccc
     9361 caactccaaa ggattttaat aattcgattc cgggtttcac tatgggaaaa tccgaattac
     9421 tccccattat aaacgctact ttatatcctc cactcatatc cttacctcca taataagaat
     9481 gcgatgcaat ttaacgcgtt gtgctatata taaaccgcct ctgtaaaaca tgcagtacgc
     9541 cttagaacag ataaataaag tccgatggta tctacatcgg actttattac acaaaatcac
     9601 gggtaactga tcaaaactaa agaattatct tcaagacaaa caatagtgca aggatgtaca
     9661 taaggggatt aatctgcttt gccttgccgg taactaactt cagaataacc catgacagca
     9721 taccgaacat aatcccgtct gcaatgctat atgccaaagg catcatgatt atggtcaaga
     9781 atgccggcag agcctctgta taatcgctga aatccacttt cgctatggcg gttaccataa
     9841 aaaggccgac agtaaccaat ataggagccg tagcaaagga cggtattatg gtaagtaccg
     9901 gcgagaatac cagcgctacc agaaacatta gcccggatac gagggcggtt aagcctgttc
     9961 tgccgccttc ggcaacaccc gatgcgctct ctacgtagct ggttaccgtg gaagtaccaa
    10021 gacatgcccc ggcaacggta ccgatggcat cggcaaggag agcctctttt gctttcggaa
    10081 gcttgccatc cttatccaaa aggttaccct tgctggcaac acctattaga gtccctactg
    10141 tatcgaagat gtctacaaat aagaatgaga acagtattac aataaattca aagcccagct
    10201 tgattgaacc ctggaaatcg aatttcaaaa aggtcgaagc tatggaagga ggcagggata
    10261 taatgccctc ctggggaatg agattaaatc tgccggcttc aatatccacc gtatacagac
    10321 cggtcaattg acaaataata cccaatgcat aagtacctaa aatgccccat agaagtgccc
    10381 cttttacgcc cttggtgttc atgtatattg tgccgattaa gcctacgaag aataaaatgg
    10441 acgtgatgtt cttcagatcg cccaatccga ctaatacggc gccgtcggct gttataattc
    10501 cggcgttttg aagacctatg aatgcgatga acaaacctat accgacagtt acggaatatt
    10561 ttagattttc cggaataccg tttacgattg cctctctgaa tttaaaaaat gacataataa
    10621 tgaatataat accctcgatc aaaacagcgg tcaacgcaat ttgccaggaa tacttaacgg
    10681 caacaacaaa ggcaaagaat gcgtttaagc ccatccctga tgcgagggca acggggtagt
    10741 ttgctaagaa cgccatggcg attgtagcga ttgcagcaga aagagcagta gcagtaaata
    10801 cagctccccg gtccatcagc tctatgccga tttcggcccc ggataggata ttagggttaa
    10861 cgattagaat gtaagccatg gtcatgaatg tagtaatacc ggccattatt tcggtcttta
    10921 catctgtttt gtgttccctt agcttaaaag ttcgatctaa aaaaccgctc atttatattt
    10981 cctcctttaa tgttatcagg tcgtatgata gtattttacc cagaaaaaat agacatatag
    11041 attcccgacc tgagtgaacc cctatgccta tatatcgcaa ggccaaaaag gcctttcaat
    11101 atcgtgcata tcacccatag cctggcagtt acggctgcct ggtagaaact cccgaaccaa
    11161 ttatcaggat tatatgagtt atatccgtta cataaataat atatcaaagc ccagtccaat
    11221 tgtcaataat aatttgaacg ttttttgttg ttatcacatt aatgttcggt tttttaggaa
    11281 ggcaacgacg ctttttggcc atttctcccc aaaatagttg ctattttccc atcaataacg
    11341 gcaataatcc tcccttgatt tgatgctttg tatgagtggg acacgcgccg gtaatgaagc
    11401 gataaaccag attaatttaa gatgaccctt cttataaaca gtctgtagac caatacgttt
    11461 aagagcagca tatagcccaa gcctataatg tagtattgat tgcacgaaag ataaaatata
    11521 ttccccctta ttaagcagct aataaccttt ccggtccaga atccgggtgc gaaggaaaag
    11581 aacaattccc ttatatcgct gaaaaagagg gcgacgatgg ggaatattat tatgctgccc
    11641 cctgccttca taacggcaaa gccttcgatt ttgtttttgg aaaaggaatt aatcaatagc
    11701 ccggccatgg gagcctctaa agatgcgaga aaggccacag ctattacatt ctttggggaa
    11761 aagtccacga tatttgaaac gacaatcgta aatagggtag ctgaaaagca gagaaagaat
    11821 accattacca acctaaagct gagaaactta tggatgctca gcggcgtagc cctaatgttt
    11881 attattatgt tatcatcccg atcctccaat atggaaaatc cgatcaaggc tccataggat
    11941 atgggtatga taagcaccat aataactaaa attaagtccg tatagggaat aaaatcaaac
    12001 ccatacctat cgcctatcca aggcaaaaga tatctgccca tcatagcaaa gaatatgggg
    12061 tagataacca tgaaacccat cattggatcc cggctccact tctttatctc cgatttaata
    12121 aaattcatat acaccctatt ccccacctcc ccttaaaaaa tgcctatgga gtttcctcca
    12181 aataagataa tgtaacagta cggagccaat tataatatag gtaacggcca gcgctatttt
    12241 ccaagattca attaccccgg tggtccctac gatgatcata agcgcagctt tagtgggaat
    12301 caagaagacc agctttttaa acaccgcatt tgttattatt gcaaattcgt caaggataac
    12361 gggtaatatt gatactaaaa aataggcaat tatgtacatt acaagatcgg taaaatctct
    12421 ggaataatag gttaacaaaa agcctatctg agaatgaaaa aatgccacca atacagtccc
    12481 gcccagcaag agaaaataat ttatatccac agttttaaag gccagggcat atatatacat
    12541 aatggacagg gaaacgatgc cgggggtggt atgggagacg agcttagcca atatgtattc
    12601 ggatttggtt atgggcgata ttagcatgct atacaaagtt gcctcgtccc gttcatatat
    12661 aaaggtaacc ccaaccagca ataccgccat ggtggtgacg tccataaaga ccaattgggg
    12721 aatcaggccc gttacattct ccatatctac aaaatgcaat accccaaccc acatcatgga
    12781 tacaaaaatg gatgccgcaa ataatttgta tcttgcaagc cttaaaagtt ccccttttat
    12841 tagagcaaaa aacctaacca ttagtatcta cccccgtcac ctttataaat atatcgtcca
    12901 aggtgctttc cctgctgtgg agggtgagga tctttctctt gtctatgata tctacaaatt
    12961 cattgttgct gcctaggtta tccaggctaa aagtggattt tgttacagaa ccgtcatcta
    13021 tgtattcaac atccaattcc ctgttactat actttaattt tagttcccta ggggtgctga
    13081 tctcgactat cttcccgtca gccatgaaag ccaccctatc gcagagttcg tccacgtcgt
    13141 tcatcaggtg tgttgtcaac aacaccgtac cgcccccctg gcgaaactct ttgattatct
    13201 ccttaatgac cctggcgttt ttcggatcca aaccgttggt gggctcatcc agaaataata
    13261 cgttggggtt atttagcatt gcccttacga agtttaacct taccttcatc ccttttgagt
    13321 attgggatac ccttctattt ctgtcttcat ataggcccaa cctctttagc agtgtgtcag
    13381 tatccatgcc tgaagcatat aactgtttaa aaaagcgtaa attttcttca gcggtcaatt
    13441 tagaaaagtg taccggcact tcaaagccga cccctatatc ctgaaagtaa ctgtccctat
    13501 aggaatgtaa atcctttccc tcatatagta tctcgccccg ataattgccc aaaagtttta
    13561 tcagaagctt ttgagttgtg gatttaccta cgccgctggg gccaaggaga ccgaatatct
    13621 ccccccttct tatatcaaag gaaatccccc ttatggtatc ctcggaattt ttcgggtatc
    13681 tgaaccttag gtccctgata tgatacaatt ttgtcacccc caattttaat tcatatgccc
    13741 taatgaatgg cagctgcaga taaacaatag gaaagctaaa tttatctata gataatggtt
    13801 tttataatcg aagctgctta tctgtaatcg gttagaattt catatggatt gaatattgac
    13861 tcctaaaaac aacaggagcc caaggccccc gtatattgta tctcgtataa tgtctcctgc
    13921 ataatgcaac ctgaagcggg ttataaacgg gtggtttatt cccactcaat cgttgccggc
    13981 ggcttgctgc ttatatcata aacaaccctg ttgacaaggg gtacctcatt ggtaatacgt
    14041 gtggatattt tatccaaaac atcatgggga atcctggccc aatcggccct catcccgtct
    14101 atactggtta cgccccttaa ggcgatggta taatcatatg ttcgttttcc ctcagttacc
    14161 cctacgcttt tcatgtcggt taaaacggcg aagtattgcc agatctccct atcaagaccc
    14221 gctatggata tctcatccct atagatcgca tcagcctctt tcaagatctc caacttctcc
    14281 cgagttacat ccccaattat ccttatggcc agcccaggtc ccggaaaagg ctgtctccat
    14341 accatatgac cgggaatgtc cagttcttcc cctacccttc ttacctcatc cttaaaaagg
    14401 tctcgtagag gttctattat ctctttaaaa tctacgtctt cagggattcc ccccacatta
    14461 tgatggcttt tgaccaagtc agattcaccc ccgccgcttt caataacatc ggggtagatg
    14521 gttccctgga ctaaaaagtc tatttcgccg agcttttttg attcttcttc aaaaatccta
    14581 ataaattcct cgccaattat cttgcgcttg atttcagggt cggatacacc cgctaagcga
    14641 tctaagaaac gatcctgggc attcacccta attaaattaa gatgaaattg gtctttgaaa
    14701 atcttttcaa cttcatcgcc ctcgtttttt ctcaacaatc catggtctac aaaaatacat
    14761 accaattgtt ctcctatcgc cttatgcatc aacactgctg caacggaact atctacccca
    14821 ccggaaagag cacataggac tctcttatct cctacctttt ccttgatgtt atcaatggag
    14881 atttcaatga aggtagccat atcccaagtg ccccagcatc cgcattcttc aaataaaaaa
    14941 tcaattaatt ggtccaagtc attccaggag tcgataacta gtacaggtac ccctatatca
    15001 taaatttgcc caaagcgttt ttgcgaatcc aattggcccg catcatcccc acgggctgat
    15061 ataattatcc ctttaggtgc gatatccctt attctttcaa cggaaacatc ggagtgaaga
    15121 acttcacaat acaccctaga ttcccttacc ttcctagcaa tatcctggct atgactaccc
    15181 ccaaagtcta aaactaacac catctcatgt ttcacgaaac tcactcccta tgttgctggt
    15241 tccttgttct ttaattaacc cccaatacta taattgggtg cttccttggt gatatatata
    15301 tcatggggat gattttcccg aagggaagcg ttggatatcc taataaatcg ggaatccctg
    15361 cgcaaggcct cgatagtggg ggtgccgcaa tagcccatgc cggctctcaa tccgcctatg
    15421 agctgaaagg ccgtatcgga aagcgatcct ttaaagggaa cgcgcccttc caccccttca
    15481 ggaaccagtt tttgggcatc ttcctggaaa tagcgatcct tgctgcctgc agccatggct
    15541 cccatggagc ccatgcccct gtataccttg aagcttctgc cctggtatat ttccatctcc
    15601 ccggggctct cttccgtacc cgcaagaagg ctgcccacca ttacgacgct ggcaccggcg
    15661 gcgatggctt tggggatatc cccggaaaac ttgatgcccc cgtcggcaat tatcggtatc
    15721 ccataaccgt ccgcaacttc agcgcaatca tgtatagccg ttatttgggg cacccctatg
    15781 cccgccacca ctctggtggt gcatatagac ccgggtccga ttcctacctt gatacagtcg
    15841 gcacccgctt caattaaatc ccgtgtagcc tcccctgtcg cgacattgcc cgctatgatc
    15901 tgaaggcttg ggaattcttc ctttatccgg cggaccgttt ttaatacgcc gacggagtgt
    15961 ccgtgggctg tatctatagc tataacgtct actttggcat ctaccaatgc ggtgaccctg
    16021 tccacggtgt ccgtggatac tcctacagcc gctcccacca gaagccttcc gccggaatcc
    16081 ttggctgagt ttggatactg gatagccttt tctatatcct tgatggtaat gagacccttt
    16141 agcatcatgt catcatcaac tatgggcaac ttttcaatct tgtgttttcg gagaatttcc
    16201 tgagcttgtt ggaggttggt tccctggggc gctgtaataa gatttttaga tgtcataaca
    16261 tcggatatcc tctgggtaaa atccgtttcg aatctgatgt cacggttggt aataatgccc
    16321 accaatttac cctttatcgt tatgggcacc ccggagattc tatactttct catgagatcg
    16381 ctggcgtcag acaacagatg ctccggtgag agaaaaaaag gatccactat taccccgtgt
    16441 tccgaacgct taaccttgtc cacctgtata gcttgtttct ctatggacat attcttatgg
    16501 ataatcccta ttccgccttc cctggcaatg gcgatggcca gccttgcctc ggtaaccgta
    16561 tccatggctg cgctcatcag cggaatgttt aatttaatgg acttggtcag attggtagta
    16621 aggtctacct gacccggtaa tacctcggat cttcccggta taagcaaaac atcgtcaaag
    16681 gtgagacctt cttttacaaa tttattattg attgcaaaca ctctccctcc ataaacatat
    16741 tgggcattcg aaagaatccc aaagccgtcc tgtaaatgtg cggccgcact tcggcacaca
    16801 tatcgcaaaa ttcgtacatc aaatctgcct ttttccctat aattattagt gaacagtcta
    16861 ccaaagtcaa tagataatgt caacggctta ttcctcgcta aaacagatgg agggttacag
    16921 agggtcaaac gctggagggt ggactttatt ccggcatact cagttgattt caccgaagta
    16981 acccggttct tcaaagaaaa ggattcaccg ccttaaaggg gctttccctg tttacttctt
    17041 ccctatctgc ttttccctgg cataatagaa taggtattgt tgtgcatatc cggcaagatt
    17101 cccaaacctg ttatctgcaa acccgcgaat atccttaaaa tctaccatgt cccgggaata
    17161 tagttcctcc ataatacgcc cgatccaaac atctacggga aaggcttggc ccttccttaa
    17221 agaaaacaat aaaatgcaat ctgcgacctt cggccctaca ccgggacatt ttaacagctg
    17281 ttctttggca ttctcataat ccatggtttt tagggcaccc aaattgattt ccccgtcacg
    17341 gatcatccga gcagtaccct ttatataacg tcccctatag ccgcaaccgc aacctctcag
    17401 ctcgctctca tccgcatcgg ctaaggcatc gacatcgggg aatctataat ggtccccgcc
    17461 ttcttgggga atccttatgc caaatctttg ggacagcttc tccaccgtcg tctttatcct
    17521 gtctatcccg ttacgggcgg atataataaa ggatatcagg gtttcccaag ggtcctggtt
    17581 tagtattctt atgccccatc catgctttat ggcttcctgc aatatcgaat ccttggataa
    17641 ttccttaatg atttccccgt aatccgtatc caaatcgaaa tagtctcgcc atatggcatt
    17701 aaaatcttta agagtcgtat tttccagtgt caaggttgcc ccgtcccaca gcatccgcaa
    17761 taccctgccg cttgcgaccc cgacgtagcc ctccccgtcc caattccaac gaaaggcctg
    17821 tccgcattca aatatatgcc tggggttaaa gttggataac ccttcaatcc tcaggatacc
    17881 cttgtttttg tcttcttgta ttttcatact cttctccctt ttacgacgga cggtgtagat
    17941 ttttcagctc tgcttctaca atggcagcaa taaatcctca cgtgccttca atctttcatg
    18001 gatagccttg gcagcctttt tcccggcacc catagcaaga ataacggtag cagcaccggt
    18061 aaccgcatct cctccggcgt agaccccatc cttactggtc gagcctgttt cctcatcgac
    18121 tataatcccg ccccaaccat gggctgcaag atcgtccgtc gtggagacaa tgaggggatt
    18181 aggactggta ccgatagcca tgattacgat atccacatcc tgtataaact cagagccttg
    18241 tattttcatc ggccttcttc tgcctgaaga atcgggttcg cctaattcca ttctgatgca
    18301 cttgagcccg gttacccagc cattatctcc catgatctct atgggatttg ccagggtatc
    18361 gaagactatg ccttcttcct tggcatgatg tatttcttcc gaccttgccg gcatctcctc
    18421 ctccgacctg cggtacacta tgcgtacctc gtcggcgccc aatctcagtg cgctacgagc
    18481 tgcatccata gctacatttc ccccgcccac caccgctact ctgtgtccta cctttatggg
    18541 cgtatggttg ttgggaaaat cgtatgcctt catcaaattg attctggtca gatattcgtt
    18601 ggcagagtat accccgttta agttctcgcc gggtatgttc ataaacctgg gcaatccggc
    18661 accgcttcca ataaaaacgg cctcgtaacc ctgttccagc agttcgtcta tggttatagt
    18721 ctttccaatg atgatattcg tatagatttg aacacctaaa tcctttatcc cctctatttc
    18781 cttcctgacc agggattcgg gaagtctaaa ctcgggtatt ccatagataa gcacacctcc
    18841 gggttcatga aaggcttcga agatatgggc ttcatatccg tatttagcca gatccgccgc
    18901 tgcggttaat cccgcaggcc cgctccccac tatggcaact tttatcccat tgcccattgg
    18961 aaccggccct ttttttgcct tttctagggc atagtcggca gcaaaccttt ctaaacgacc
    19021 tatggctacc ggttcgccct taatacccaa gatacactta acctcgcatt gctcctcctg
    19081 aggacaaact cgaccgcaaa tagccggtaa gttgttggtc tccttgatta tttccgcagc
    19141 cctgtcaata ttaccgttgg ctatatggtc gataaattcg ggtatgcgta tatttaccgg
    19201 gcatccgtca acacacctgg gcctcttgca ttgtatacat cgcttcgcct cttgtatggc
    19261 ctcttctaag gtatatccta aagaaacctc gttaaagtta tgcttccgga cctcgggttc
    19321 ctgttccccc acgggcactc ttgtaagaac cagactcatg tttccttccc cctaatctaa
    19381 actgtatatg ttgcgcaaaa tgcaacctat ctccggcttt ataatgaccc ggctttccca
    19441 taatatatgg gtaattattc gtttggcaac agcctgcagc tatgctcaac cttttcttcc
    19501 tctttataca tttgctggcg tcttatagcc tcatcaaagt ctaccagatg cccgtcgaag
    19561 tcaggtccgt ccacgcaggc atagaagacc ttgcccccaa cagtaaccct acacccacca
    19621 cacattccag taccgtctac cattatgggg ttcaggctca ctgttgtggg tatgttgtat
    19681 tctttggtta tggaagaaac gatcttcatc ataataagcg gaccgactgc aattaccaga
    19741 tcatatctgt taccctcatc tattaatcgt ttgaccacat ccgttacaaa gccctcatga
    19801 cccgaggatc cgtcgtccgt agccacataa acattggaag aacaagcctt aatctcgtct
    19861 accaggatta gacaatcgga atttcttgcc ccgattatca catccacatc cgtaccccga
    19921 ctatttagat atttaatttg gggaaatagg ggggctatgc ctacaccgcc gccgacacac
    19981 aaaaccctgc catggttatc caatttagat gccttgccca atggaccggc aaaatccctt
    20041 atgctgtccc ctacaccaag catacccaag tccctggtcg tgtagccgac ctcctgaaag
    20101 ataattgata tgcttccctc ttccctgtca taatccgcaa tggtcagagg aatccgctcg
    20161 ccctcttcgc tgacccttag catgataaat tgtccaggca tggcttttcc ggcaaccagc
    20221 ggcgccttca ccttcatcaa tattatgcct ggacctatgg ttcttttatc cattatttca
    20281 aacatggctt atacctccta tgcagtatta aagttccccg ttacacaatt tgtaatatac
    20341 gcgacagctg ttgttttcct gttggcatgt attttcaact attttctata acgatgcttt
    20401 tggccgttac atttacctgg agcacattga ttccagttaa gtgtactata tcattggata
    20461 tatttccttg gatttttcca atgatttcat gtattttata accgtaaacc acggtcattt
    20521 ccagatttat taccacaccg cctgcatgac ttatggaggt agccttggtt accctatata
    20581 ccccttctac cttgtatgca ttgtgcgatg cgatcgattc cagagctaaa tcagctataa
    20641 agaattttcc catatagcta tacgtgggcc gcataaccgt cttttccaga gtctctattt
    20701 tcctgcccct ctcggtaaat ctgagaattc gcaatggatg aagaagatac cctgaaaaat
    20761 cctggcgtat ttccagcgct gggaccggga taacatgctt gccctgctcc cttctgatag
    20821 tacgggcggc ctgtatctcc tcctcggtgg aaatatcctc tattttaatt atctcttctg
    20881 caggcggtaa ccgaagattg ttgacgattc tatgaatcat attaacagat gtgcctaaaa
    20941 ttaatatggc atcgggcccg gctatatcaa tggattcctt tacgccggat gcatgtaccc
    21001 tatccatgaa taatgccctt cgcacagacg ccaccatggt agcctctctt tttgcagaga
    21061 tcccggcaat taccttactg cccttaatta ataatccgtc gtctataat
//
LOCUS       DASRNX010000248         8839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_581985,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000248 DASRNX010000000
VERSION     DASRNX010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581985"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFD89_09920"
     CDS             complement(<1..182)
                     /locus_tag="VFD89_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58534.1"
                     /translation="MLSNKKTEFTDKEILEKGTRALIKELGYSGFLRFIRQTEGGSKD
                     DYLKINEKIFEGMSIN"
     gene            complement(169..645)
                     /locus_tag="VFD89_09925"
     CDS             complement(169..645)
                     /locus_tag="VFD89_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HZJ58535.1"
                     /translation="MKIYFDMNIYNRIFDDQTQVRIRFESIATDILFELIEKGQYSLV
                     WSFILDYENSRNPYIERKTYISSISSLCKEIVEPNNNVNLIAKAIIENSSASAKDALH
                     LACAVYGKSDFFITCDDKLINTIKRNQNRLEGIIGDIKLYNPIDFLRKEIDVDVIE"
     gene            complement(879..1097)
                     /locus_tag="VFD89_09930"
                     /pseudo
     CDS             complement(879..1097)
                     /locus_tag="VFD89_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
     gene            complement(1650..1862)
                     /locus_tag="VFD89_09935"
     CDS             complement(1650..1862)
                     /locus_tag="VFD89_09935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HZJ58536.1"
                     /translation="MSVITYGLIKKHGFVDGNKRIGVSIMLLLLRISDIQIHYTQNEL
                     AQLGLGIADGSLDKKEINKWIKNHIL"
     gene            complement(2120..2518)
                     /locus_tag="VFD89_09940"
     CDS             complement(2120..2518)
                     /locus_tag="VFD89_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58537.1"
                     /translation="MKWKMGLTLGSKQKEFRWRYSKANGLVIIRTFKNGNKKTEFSNE
                     DIDSIINFIKEEGIVPLANNVVKLSNGTEKEGIGRFIYQNISNDTTNAQTASQLASIL
                     YNTGVLGYNGAVRGMEFWILDDNWKEKLEK"
     gene            complement(2817..5351)
                     /locus_tag="VFD89_09945"
     CDS             complement(2817..5351)
                     /locus_tag="VFD89_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HZJ58538.1"
                     /translation="MLKISSIRVDGMEQGLVTDTLPNITFELDSSLQGDGLRSALITI
                     DDWKLETTSQINNIYDGPMKPFKQYTVHISVQGIKGGTAQAWTTFETGRLETPWKAKW
                     ITDLSYDFPKNTSPIPMTFRKYCNIDKKIHRAFFNVTALGVYELNLNGEKVGEDYFAP
                     GLTSYQHQIQYQTYDVTDELRDENKILAVVAGGWAAGSFTYRRKSKISADRQALLCEL
                     RIEYQDGSLEIIGTDENWEVTLDGNYRMAEWYDGETYDARLDLNKSQWRKTDITHPRK
                     NPKILAQYGPSVRVMGELSPISVNRAKSDELIYDFGQNFSGVIRAKIHGREGQTITFR
                     HAEVLVEGELFVKSLRTAKATATYICVDGEQEYSPKLTYMGFRYVGVQGIALEDIELS
                     ALVLHSDIEEVGSFSCSNELINRLQENIRWGSKSNFVDIPTDCPQRDERQGWTGDIAV
                     FAGTACFNFDMGRFFDKWLTDMRSEQGRGGGIPMVIPKAGDAWPAMATSCWGDSCILV
                     PWAEYMARGNENVLRKQYPTMRRFLKAAKWWSGFLSFTPKGRYIWRWPFHFGDWCAPE
                     GSAKDWISKGKWIGTAYFANSCGIVAKIAEILGYDKDAEYYRGLREKIIQAYRHVFTD
                     GQGKLKNEFQTAYVLPLHFEMTRGAETEAMADNLVKLIKDADNHLGTGFPGTPYILFA
                     LSDNGQLDGAYDLLLQDTCPSWLYQVKVGGTTIWERWDALRPDGSVNIEGLSQSEDES
                     SGGMVSFNHYAGGAVGDWLYRRIAGIEPTSGGYKAFKVAPKPGGGLSWAKATHRCPYG
                     EIVSQWKIEDGVFTLYIKVPCGTRAQVVMPNGEEYEVESGEHTFSI"
     gene            complement(5355..7610)
                     /locus_tag="VFD89_09950"
     CDS             complement(5355..7610)
                     /locus_tag="VFD89_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZJ58539.1"
                     /translation="MIDKMKLRDKVALCEGKNFWETKGFPQYGIPSMVMCDGPHGLRR
                     QEDHSDHLGVNKSRPATCFPAAVSTACSWDVDLVGEIGSAIAKEAADQGVGVFLGPGA
                     NIKRNPLCGRNFEYFSEDPYLTGKLAAGFIKNAQAKGIGTSLKHFACNNQEFKRFNSN
                     SILDERTLREIYLAPFEIDVKEGNPKTIMCAYNKINGIYCSDSEELLTQILREEWGFR
                     GLVMTDWGAMHNRIQAFKAGCDLNMPGGSNYMEGEVLDAVTGGELDKTYIHQSAERVK
                     NMVMEAQAALKDKDGCDYDANHKLACIAAGQSAVLLKNQDNILPLRKEEKIAIVGSMV
                     ENMRFQGSGSSHINPTRLINPNQVFENTVDVEEADIAIVLTGLPSEYESEGFDRDNMM
                     MPQEELELIQETADKNPDTIVVLFCGSPVETPWADKVKGILYMGLPGQAGGEALKSLL
                     YGDVNPSGKLAETWPIKHEDCPSSTYYPERDGQYREGIYVGYRYYDKANVPVRWKFGF
                     GLSYTEFAYSNPEIDGDTVRVIVRNTGSRWGAEVVQLYISPPQDGIHRPIRELKGFTK
                     IDLQPGERREVIFHLDKRSFAIWDNGWKVAPGSYTILVGGNPDKLMVAGSIEKSGQTV
                     KISDWQSGSWYEKPDGAPSLKEWEKVIGHRYVPYTPKKGKFTMNDTVMDMKEHSLVMR
                     MMYWGVKKTISKGAKPGTPEFRMLMESSAGSPLRSMQISGGMKAGLFKGLLAMANGRF
                     FSGLRILLRGE"
     gene            complement(7674..8243)
                     /locus_tag="VFD89_09955"
     CDS             complement(7674..8243)
                     /locus_tag="VFD89_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZJ58540.1"
                     /translation="MISVIGGIPMGIGIFAVWPLAKRFGKRNVTMAGFVLYAIGGLIC
                     LLDPRNMTIVLIGQFIKNIGGLPSAYVFLALFADVLDHMEWKAGFRVDGLSASVNTII
                     VTVCYGLSSGLFNMLLARTGYIAPVLIEGKTVATAQNPATQGVITFSFLGMEVITAII
                     LVALLAFLSVEKVIDKEQKDIKARLEKDV"
     gene            complement(8472..8654)
                     /locus_tag="VFD89_09960"
                     /pseudo
     CDS             complement(8472..8654)
                     /locus_tag="VFD89_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
BASE COUNT         2371 a         2174 c         1553 g         2741 t
ORIGIN      
        1 tcatttatgg acataccctc aaatattttt tcatttattt ttagataatc gtctttgctc
       61 cccccctccg tttggcgaat aaatcttaag aatccagaat agccgagctc ttttatcaac
      121 gcccgtgtac ctttttctaa tatttcttta tcggtaaatt ccgtcttttt attcgataac
      181 atctacatca atctccttcc ttaaaaaatc aattggatta tataatttta tgtccccgat
      241 tattccttca agcctgttct gattcctttt aatcgtattg attaatttat catcacaagt
      301 tatgaaaaaa tccgacttac catatacagc acacgccaaa tgcagtgcat ctttggctga
      361 agcagaagaa ttttcaatta tagcctttgc gatcaggtta acattattat tcggttctac
      421 aatttcttta cataacgatg aaattgagct aatataggtt ttcctctcga tgtagggatt
      481 cctgctgttt tcataatcta atataaagga ccatacaaga ctatattgcc ctttttcaat
      541 gagttcaaac aggatatccg ttgctataga ttcaaaccgt attctaacct gcgtttgatc
      601 gtcaaaaatt ctattgtata tattcatgtc aaaatagatc ttcatcgctg tccccaccac
      661 atccttttaa tggataccct gattatacca taggcaatta tcaaattcca tatttgtaag
      721 ccttcgatcc tttggaaagt attattcgcc aaagtattat tcgtgtaacc caaacgggac
      781 ctattttggc taaatccttg agattgtatg gcgcggatat cataagcact ttaggcgtac
      841 aatacgtatg taagagatac attgaaatct tatagtcatt gatataactg tgttttcaaa
      901 aagtttattt tatgaacatg gcaccagtga caggcggaat agccaatgga tagaaagatt
      961 ggcttgtatt cttctttggc tttttcaaag gctttatcag accaaggata ccagtcaacg
     1021 gggttatagg cgtgttgtag caggtaagga cttttttctt ctattaatct atttgatgct
     1081 ttatttgaat tagtcatatt attaggctcc tccatggtat attggtaata ttataccctg
     1141 aggtgtggct ctatccgaat atattcctgc taggatcgcc atctaaggat cctttttatt
     1201 cgttttatta taaatggtat atagcagcgg ctgtaatgag ttggaaaaat cgcatccttt
     1261 aaaagtcatt tgctaaaatg gttaaattga taatattata ccccggaaaa tggatgttct
     1321 atccgaatat ctttgtggcg tgatatttga caatcacata gctcttaaac tcacgatagg
     1381 tgttagatat ccatccatgg ctactcccgc attattaatg atccccctgc agtcttatat
     1441 aaatagtgtg cagcattaca atctgtcatt ctgaggagca tagcgacgaa gaatctttgc
     1501 tttaccccag gattcctcac attctttcgg aatgacataa cgcttatgtc gcttaattat
     1561 actaatacgt tatatatcct aaggtagatc tcactttact atcgttccag aacattcctg
     1621 aaaaaactgg gcctacatca ggagctcctt tataatatat ggttttttat ccatttgttt
     1681 atttcttttt tatcaagtga cccatcagct atacccaatc ccagttgtgc taattcgttc
     1741 tgcgtatagt gaatttgaat atcacttatt cgtaataaca atagcattat tgatacacca
     1801 attcttttgt tcccatcaac aaacccatgc ttttttatca atccataggt tataacagat
     1861 attttgtcct catttcagga gctgtggtag caaaagaatc cttcaggata ctttcttcaa
     1921 tattgtcccc acaagcgtgc tctacctgat aatcaagatg tgtaccgact tctcttataa
     1981 catcatctga ggaccataac cataacctag catatgattc taagaactta tttttcagtt
     2041 cagtattatt gctcctacag gagataaaga catttaacga aactatcatt atgtaccata
     2101 atcacagctt tttgctatgc tatttctcta gcttttcctt ccagttgtcg tctaatatcc
     2161 aaaattccat tccacgtacc gcaccgttat acccaagtac tcctgtatta tatagtatgg
     2221 atgctaattg acttgctgtc tgagcattag tcgtatcatt ggatatgttt tgatatataa
     2281 acctaccaat tccttctttt tctgttccat ttgatagttt taccacatta ttagcgagag
     2341 gtactattcc ctcctctttt ataaaattta taatgctatc tatatcttca ttagaaaatt
     2401 ctgttttttt attaccgttc ttaaaggtac gaattattac taacccgttg gctttagaat
     2461 atctccacct aaattctttc tgttttgaac caagcgttaa tcccattttc cacttcattc
     2521 actgcacccc ctaatttcta atcctcctta tttgatacaa ttttactgtt gcatataaga
     2581 ttgtctatgc ttaatatcca tgattccgta gccaaggtta atcaatttta tcgtcaagtg
     2641 gcccgctaaa ctccttcaga aacttttcag ccaaacccaa gtttcacgcc ccactctcta
     2701 tgcttttgca gtccaattga atatagcaag ccctttccta aaccaatcta tactactata
     2761 tggacaacct gatctctgct ttcagttgat cccccttacc tacagtggtt taacatctaa
     2821 atactaaaag tatgctcacc gctctccact tcatattctt caccattggg cattaccacc
     2881 tgtgccctgg ttccgcatgg cactttgata tagagcgtaa aaactccatc ttctattttc
     2941 cactggctga cgatttcacc gtatggacag cgatgtgtcg ccttggccca gcttaaaccg
     3001 ccaccaggtt ttggcgcaac tttaaaggcc ttatacccgc cactagtagg ctcaattcct
     3061 gctatccttc tatacaacca atcccctact gcaccgccgg cataatggtt aaagcttacc
     3121 atccccccgc ttgactcatc ctcagattga ctaagaccct ctatattaac tgagccatca
     3181 ggacgcaggg cgtcccatct ctcccagatt gtagtcccgc caaccttaac ctgatacagc
     3241 caagaggggc aagtatcctg caagagcaga tcgtaggccc catccaattg cccgttatcc
     3301 gatagagcaa ataagatata tggtgtcccg gggaaaccgg tgcccaaatg attgtccgca
     3361 tcttttatca gcttcactag gttatcggcc attgcctcag tttccgctcc ccgagtcatt
     3421 tcaaaatgca agggcagcac ataagcggtt tggaattcgt ttttgagttt accctgacca
     3481 tcggtgaaca catgccggta ggcttggatg attttctcac gtagccccct ataatactcg
     3541 gcatccttgt cgtagcccaa aatctctgcg attttggcta caatgccgca ggaattggca
     3601 aagtatgcgg taccaatcca tttaccctta gatatccagt ccttggcact cccctctgga
     3661 gcgcaccaat caccgaaatg aaagggccag cgccagatat accggccttt aggggtaaag
     3721 gataaaaatc cagaccacca cttagcggcc tttaggaacc tcctcatagt cggatattgt
     3781 tttcggagta cgttttcatt accccttgcc atatactctg cccaaggaac cagtatacag
     3841 gaatcccccc agcaggatgt agccattgcg ggccatgcat cccccgcctt cggtataacc
     3901 atgggtattc cgccgccccg cccttgttcg gaacgcatat ccgtcagcca cttgtcaaaa
     3961 aatctgccca tatcaaaatt aaagcaggcg gtgccagcaa agaccgcaat atccccggtc
     4021 caaccctgcc gttcgtccct ctgaggacaa tcagtgggaa tgtctacaaa attggactta
     4081 ctgccccaac gtatattctc ctgcagacgg tttatcaatt cattggaaca cgaaaaggag
     4141 cccacctcct cgatatccga atgaagaacc aaggctgata gctctatatc ttctagggca
     4201 atcccctgaa cccctacata gcgaaaaccc atataggtta gtttcggaga atactcctgc
     4261 tctccatcta cgcatatata ggtcgcagtg gcctttgctg tgcgtaagga cttaacaaag
     4321 agttcaccct ctaccagcac ctcagcatga cgaaatgtta tagtttgtcc ctctctacca
     4381 tgaattttgg cacggattac accggaaaaa ttctgcccaa aatcgtagat aagctcatcg
     4441 cttttagctc tgttaacaga gattggggac aattctccca taaccctaac cgaaggaccg
     4501 tattgtgcta aaatttttgg atttttcctt gggtgggtta tatctgtttt tctccactgt
     4561 gatttattaa ggtccaacct ggcatcatag gtctctccgt cataccattc tgccatccta
     4621 taattgccat ccaatgtcac ttcccagttt tcatcagtac caataatctc tagggaaccg
     4681 tcttggtatt caattcggag ttcgcatagc aatgcctgcc tatccgcact tatcttgctt
     4741 tttcgcctgt aggtaaagga tcctgcggcc caacctccgg ctaccacagc caaaatttta
     4801 ttttcatccc tcagctcatc tgtcacatca taggtctgat actgaatttg gtgttgatag
     4861 gaagtcagcc ccggtgcaaa gtaatcctca cctaccttct caccgttaag atttaactca
     4921 tataccccca aggcagtgac attaaagaag gcacgatgga ttttcttatc tatattacaa
     4981 tacttccgga atgtcatggg gatcggtgaa gtgtttttgg gaaaatcata gctaagatcg
     5041 gtgatccact tcgctttcca gggggtttcg aggcgtccgg tttcaaaggt tgtccatgcc
     5101 tgtgcagtcc caccctttat gccctgtacg ctaatatgaa cagtgtattg tttaaagggt
     5161 ttcataggcc cgtcatatat attattaatc tggctagtgg tttccaactt ccaatcatct
     5221 atggtaataa gggcacttct tagcccatca ccctgcagcg aagaatctag ttcaaaagtg
     5281 atgtttggca aagtatcggt aaccaatcct tgttccattc catccactct aatagatgat
     5341 atctttagca tatatcattc accccttaat aaaattctta gcccgctgaa aaacctgccg
     5401 ttagccatgg ctaatagccc cttaaatagg ccggctttca taccgccgga gatctgcata
     5461 ctccggagcg ggctgccggc actggactcc ataagcatcc gaaactctgg tgtacctggc
     5521 ttggcaccct ttgaaatggt cttttttaca ccccaataca tcattctcat aactagggaa
     5581 tgctctttca tatccataac cgtatcgttc atagtaaact tgcccttttt cggggtataa
     5641 gggacgtacc tatgtccaat cactttctcc cattctttga gagaaggtgc cccatccggc
     5701 ttttcatacc aagaacccga ttgccagtcc gaaattttta ccgtttgacc agatttttcg
     5761 atggatccgg ctaccatcag tttatcggga ttgccaccta caaggatagt gtagctccct
     5821 ggagccacct tccagccatt atcccagata gcaaaggaac gtttatctaa gtgaaatatt
     5881 acctccctgc gttccccagg ttgcagatca atcttggtga aacccttaag ttcacgaatt
     5941 ggtcggtgta ttccatcctg aggcggggaa atatagagct gcaccacttc agccccccaa
     6001 cggctaccgg tatttcttac aataactcga actgtatcac catcaatctc tggatttgag
     6061 taggcaaatt ccgtatagct taacccaaaa ccgaatttcc agcgcacggg cacattggcc
     6121 ttatcatagt atctatagcc cacatatatt ccctcacgat attgcccgtc ccgctcggga
     6181 taataggttg aagagggaca atcttcatgt tttatgggcc aggtttcggc gagcttaccg
     6241 gatggattga catcaccata gagcaggctt tttaaagctt cgccccctgc ttggcctggc
     6301 agccccatat acaagatccc ctttacctta tccgcccagg gtgtctccac aggtgaaccg
     6361 caaaagagca caactatggt atcagggttc ttatctgccg tctcttggat aagctccaat
     6421 tcttcttgcg gcatcatcat attgtcacgg tcaaatcctt cactctcata ctccgacggc
     6481 aatcccgtta acactatggc tatatcagcc tcttcgacat ccacagtatt ttcaaagact
     6541 tgattgggat taatcaacct ggttgggtta atatgacttg aacctgaacc ctgaaaccgc
     6601 atattctcta ccatacttcc cacaattgct atcttttctt cttttcttaa gggtaaaata
     6661 ttatcctggt ttttaagaag cactgcactt tgtccggcag ctatacaagc tagcttgtga
     6721 ttggcatcgt aatcgcagcc atccttatcc tttaatgccg cctgggcctc cataaccata
     6781 ttttttaccc gctctgcgct ttggtggata taggttttat ctaattcgcc cccggttaca
     6841 gcgtctaaaa cctccccttc catataatta cttccccccg gcatattcaa atcacaacct
     6901 gccttaaagg cttggattct gttgtgcatg gctccccagt ctgtcataac tagaccccta
     6961 aagccccatt cctcccgcaa gatttgggta agtaactctt cactgtcact acaatagata
     7021 ccatttatct tattataggc gcacattatg gtttttgggt tgccctcctt tacatcaatt
     7081 tcaaaggggg ctaggtagat ctctcgcagc gttctctcat caaggataga atttgaatta
     7141 aatcgtttaa actcctgatt attacaggca aagtgcttaa gagaagtccc tatacccttt
     7201 gcctgagcat tttttataaa gccggctgct agctttccgg taaggtaggg atcttcggag
     7261 aagtattcaa aatttctacc acatagggga ttgcgtttta tattagcacc aggacccaga
     7321 aatacaccca caccctgatc tgccgcttcc tttgcaatgg cagaaccaat ttcccccact
     7381 aaatctacat cccaactaca tgcggttgat actgccgcag gaaaacatgt agcaggccgg
     7441 gatttgttca cgcccagatg atcactatga tcttcctgcc tgcggagccc gtgagggccg
     7501 tcacacataa ccatagatgg gataccgtat tgcggaaatc ccttggtttc ccaaaaattc
     7561 tttccttcac acaaggcaac tttatccctt agcttcattt tatctatcat accttccccc
     7621 ttagttattt tgctaaaata cagaaccgtt ttcccgaatt gatacttaaa ctcctacaca
     7681 tccttttcca gcctcgcctt aatatctttc tgttccttat caatcacctt ttcaaccgat
     7741 agaaacgcta aaagggcaac cagtataatc gctgttataa cctccatccc cagaaaacta
     7801 aaagtaataa cgccctgggt ggcaggattc tgggctgtag ctacggtttt accttctatc
     7861 aatactgggg ctatatatcc ggttctagcc agcaacatgt tgaacagacc gctagaaagg
     7921 ccataacaaa cggtaacaat aatggtattg acagaggctg acaatccgtc aactcgaaag
     7981 cccgccttcc attccatatg gtctaataca tcggcaaaga gcgccaaaaa cacataggcg
     8041 ctgggtaagc ccccaatatt tttgataaac tgcccgataa gtactattgt catgttacgt
     8101 gggtctagca aacatataag gccacctatt gcgtagagta caaaacctgc catggtcaca
     8161 ttccttttac caaatctttt ggccaggggc cacacggcga atatcccaat ccccatggga
     8221 attcccccaa taaccgatat catggtctga gtgataccat attataccat aatcacagct
     8281 ctttgattgg aatgttgtgt ataaaattct aactatatgg gatattcaac cccataattg
     8341 tcagtagcat tcccgattca tgagagatat tacattggct gctaaattta agcgtataat
     8401 gtgtatgcat gaggaattat gatcccctac aaaagattga tagatcccac tctctgctag
     8461 tttatttcat aaacatggca ccaatggcag gtggaatagc cgatggataa gaagatgggt
     8521 ttgtcttctg atttggcttt atcgaaggct tcatcggacc aggggtacca gtctacgggg
     8581 ttatttgcgt gttgtagaag ataagggctt ttttcgttta tcaatctatt ggatacttta
     8641 tttgaattgc tcatattttt aggttcctcc atgatatatt ggtaatatta taccctggag
     8701 aagttccatt ctatccgaat gttttcgtga cgtgatatta gataataaaa aggccgtaaa
     8761 ccccataagg tattaggcct ccatctataa tctgcttccc ctattcctaa taatcccctt
     8821 cattcgcata ctataaatg
//
LOCUS       DASRNX010000249         3719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_582124,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000249 DASRNX010000000
VERSION     DASRNX010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582124"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1139)
                     /gene="gpr"
                     /locus_tag="VFD89_09965"
     CDS             complement(159..1139)
                     /gene="gpr"
                     /locus_tag="VFD89_09965"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HZJ58541.1"
                     /translation="MKILTSIRTDLAMEARELYGEQSNDMEGIESENHESDGIEITRV
                     KITSPVGEAKIGKPMGTYITIEAEDIKNGDVEFTELVSQKLGQEIKSLVDLGKDSNVL
                     VVGLGNWNITPDSLGPKVVNRTMITRHIIELIPDQVDERVRSVCAIAPGVLGITGIET
                     GEVVKGLVDRIKPDLVIVVDALASRKTQRIGTTIQIADTGISPGSGIGNKRMGINEEN
                     LGIKTIAIGVPTVVFAHTIGRDSLELLIEEFANQTAPGTAFYKIIREMPENQLDAMVN
                     QVLSQGLGDLVVTPKEVDMLIDDISQIISDGLNLALHEDITLEESRRFLQ"
     gene            1395..1667
                     /gene="rpsT"
                     /locus_tag="VFD89_09970"
     CDS             1395..1667
                     /gene="rpsT"
                     /locus_tag="VFD89_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019391417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZJ58542.1"
                     /translation="MANIKSSIKRIKIANIRTMRNKSIKSGFRSAIKNFEIAISEGNL
                     DNARALYPKISSKLDKAAAKGVLHRNAAARKKSKLALKLKTAQAQE"
     gene            complement(1737..2750)
                     /gene="holA"
                     /locus_tag="VFD89_09975"
     CDS             complement(1737..2750)
                     /gene="holA"
                     /locus_tag="VFD89_09975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZJ58543.1"
                     /translation="MDYRRIFKNIKAGGWQGVYLFFGEEEYIKQEALNQTIETLVDPS
                     LRDLNYAQMDGSLVDLDYIINACETLPFMADKRLVVIKDLPVLKGRSDGGIDETGFLD
                     YIKGMSETTCLILYCRDSVDKRRKIYRSIKSTDKALEFRHIKGRELYTWINQTVERHG
                     KRISFNDANHLVDLVGNNLEDISNELKKLISYVGRDTEINVGAMDQVIVPTLEQSIFQ
                     LVDAIGEKRAGEALSVLGNLIYGGEQAIQPILAMIARQFRLILQSKGYHNMGYTYEAI
                     AAKLKQRLFVVRKCLAQGRNFTVEQLKKGLNLCLETDYEIKLGRIKDTMALELIIIKM
                     CSQ"
     gene            complement(2799..3179)
                     /locus_tag="VFD89_09980"
     CDS             complement(2799..3179)
                     /locus_tag="VFD89_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZJ58544.1"
                     /translation="MDRIKLGRWGEERAGEYLADQGLVVREKNFRCKLGEIDLIAMDG
                     ECLVFIEVKTRTSVAYGFPMEAVGRRKQEKYIQMASVYIKARGLYGVPFRFDIVEVLT
                     RDYEPPIINHIPNAFQSTGGRYYL"
     gene            complement(3234..>3719)
                     /locus_tag="VFD89_09985"
     CDS             complement(3234..>3719)
                     /locus_tag="VFD89_09985"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZJ58545.1"
                     /translation="DPDTYIDGVNDSKKLSPSKREKLFQIIMDKAKAVGVGRVGPREI
                     DRINILEATRLAMEYAASQCYPDIDFLLVDAMDGNFPVPYSPIIKGDNRSHSIAAASI
                     VAKVTRDREMAQWHRKYPQYGFNRHKGYGTGGHMDKIRQIGPCSIHRRSFIHKLVNAR
                     Q"
BASE COUNT          972 a          913 c          666 g         1168 t
ORIGIN      
        1 aggagaagaa tatcattctt ctccttgttt tattttttaa attatttttc agtctgctca
       61 tggcctgctc ccatcagata atttagccgc tgaaagtgga tgcaatcctt acgcaaagct
      121 atagcagcga aatactttaa cggcgatgta gttacatttc attgaagaaa gcgccgggat
      181 tcttccaggg taatatcctc gtgtagcgcc agatttaatc cgtcggatat aatctgtgat
      241 atatcatcta tgagcatatc cacttctttt ggcgttacaa ccaaatcacc gagcccctgg
      301 ctgaggactt gatttaccat agcatccagc tggttttcag gcatttcccg aattatctta
      361 taaaatgctg tgcccggtgc ggtttggttg gcaaactctt caattagcag ttccagagag
      421 tcccttccaa tagtatgggc aaacactacc gtggggaccc cgatggctat ggtctttata
      481 cccaaatttt cctcgtttat tcccattctt ttgtttccga tgccggatcc cggactgata
      541 cccgtatcgg ctatttgaat agtcgtacct attctttggg ttttgcgaga tgccagggca
      601 tccacaacaa ttaccagatc aggcttaatc ctatctacca aacccttgac tacttcgcct
      661 gtttcaatgc cggttatacc caatacaccc ggtgctatgg cacatacaga acgtaccctt
      721 tcatccactt ggtccggtat aagttcaatt atatggcgcg tgatcatggt cctgttaact
      781 accttcggac ccaatgaatc cggtgtaata ttccaattgc ccagtccgac caccaataca
      841 ttgctatctt tacccaaatc gaccagggat ttaatctcct ggcccagctt ttggctgacc
      901 aattcggtaa attccacatc gccgtttttt atatcctcag cttctatggt tatataggtc
      961 cccatgggct tacctatttt agcttcaccc acagggctgg tgatcttaac cctggtaatc
     1021 tcaataccgt cactttcatg attctcactt tcgattcctt ccatgtcatt gctctgttct
     1081 ccgtatagtt ctctggcctc catggccaaa tccgttctaa tactcgtcaa tatcttcacc
     1141 tctcaaaatt ttagcattct ttgcaccggc aaaagccata cccattgggg atatggtata
     1201 gtgctatgat atccccagcc ctttaccaat tgaaatcaaa aggtttccat atttctatat
     1261 tcctatattg accacatgga acttttttat acgattacaa cctggttacg ggtaaaaatg
     1321 ctacattttc cttgcctttt ccgatttccc atgataaaat tatcttcgtt gattcgttca
     1381 aggaggtgaa ttgcttggct aacattaagt cttcaataaa aagaataaaa atcgcaaata
     1441 tcagaacaat gcgaaataag tcaataaaat ccggctttag aagtgccatt aaaaactttg
     1501 agatcgctat ttctgagggg aatttggata acgcccgtgc cctatatcct aagatcagca
     1561 gcaaactgga caaagctgct gcaaaagggg tgttgcatag aaatgccgca gccaggaaaa
     1621 aatccaagct ggctttgaaa ctaaaaacgg cccaagccca agaataattg aaaaaacttt
     1681 ggcaaaaagc ttcccggaga aggaagcttt tgttatctgt gtattttgaa ggcccattac
     1741 tggctgcaca tcttaataat aatgagctcc agtgccatag tgtccttgat cctacccagc
     1801 tttatttcat agtctgtctc cagacacaag ttcagccctt tttttagttg ttctacagta
     1861 aaattcctgc cttgggccaa acattttctt acgacaaata atctttgttt cagttttgcg
     1921 gctattgcct cataggtata acccatatta tgataacctt tggattggag tattaaccta
     1981 aactgtctgg cgatcatggc cagaattggc tgtatagcct gttcgccccc atatatgaga
     2041 tttcccaata cgctgagagc ctcaccggcc cttttttctc caattgcatc caccagttgg
     2101 aagatactct gctcaagtgt aggtacgatg acttgatcca tagctccaac attgatttcc
     2161 gtgtcccttc ctacatagga aattaatttt ttaagctcgt ttgatatgtc ttccaaatta
     2221 ttgcccacga ggtcaactaa atggtttgcg tcgttaaaag aaattctttt cccatgcctt
     2281 tctacggtct ggttaatcca ggtgtataat tccctgccct taatatgccg gaattctaag
     2341 gctttgtctg tactcttaat agacctatat attttccttc ttttgtctac gctatcgcga
     2401 caatacaata tcaagcaggt ggtttccgac atacccttta tatagtcaag aaacccggtc
     2461 tcatctatac caccgtcgga tcttcccttt aaaactggca gatccttaat tactaccaac
     2521 cgtttatcag ccataaatgg cagagtctca catgcattaa ttatgtaatc taaatcaact
     2581 aaagagccgt ccatttgggc atagttaaga tccctgaggg aagggtctac taatgtttct
     2641 atcgtctggt tcagggcctc ttgttttata tattcttcct cgccaaaaaa aaggtagaca
     2701 ccctgccaac cccccgcttt tatgttctta aagatcctcc gataatccac ctgtatcccc
     2761 ctaaactcca tgggacgttt atatttgttc gacccgattt ataaataata cctgcccccg
     2821 gttgattgga atgcattagg gatatgatta attattggcg gttcatagtc ccttgtcaaa
     2881 acctccacga tatcgaatct aaaaggtacc ccatacaatc ccctagcctt gatataaacg
     2941 gaagccattt ggatatactt ttcctgtttc ctcctgccga cagcttccat gggaaatcca
     3001 tatgcgacag aggtccgggt ctttacttca ataaatacca ggcattcccc gtccattgct
     3061 attaagtcta tttctcccag cttacagcga aaattctttt cccgcactac aagcccctga
     3121 tccgccaaat attcaccggc tcgttcttct ccccatctgc ccaattttat acgatccata
     3181 ttatctgttc tcccaacttt atgcgcatcg attgaaaggg aatcccgtgt ttgttattgc
     3241 cttgcgttaa ctaatttatg aatgaagctg cgcctatgaa tagaacaggg tccaatctgt
     3301 ctgattttgt ccatatgacc gcctgtccca tagcccttat gtctattaaa accatattga
     3361 ggatattttc tatgccattg ggccatctcc ctatccctgg ttacctttgc gacaattgaa
     3421 gcggcagcta tagagtggga acgattgtcc ccttttataa tgggtgaata tggaacagga
     3481 aaattgccat ccatagcatc caccaacaga aaatctatgt cgggataaca ttgggatgca
     3541 gcatattcca ttgccaagcg ggtagcctct aagatattga tcctgtcgat ttcccttggc
     3601 cccacgcgtc cgacgcctac cgccttcgct ttatccatta tgatttggaa gagtttttcc
     3661 cttttagagg gggaaagctt ctttgaatca tttacaccgt ctatatatgt atccggatc
//
LOCUS       DASRNX010000250         7072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_582224,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000250 DASRNX010000000
VERSION     DASRNX010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582224"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFD89_09990"
     CDS             <1..717
                     /locus_tag="VFD89_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HZJ58546.1"
                     /translation="IEDNKPDAIAAMEEAITDEPNITIKKLRTKYPQGCERKLIETIT
                     GRQIPSGGLPMDVGAIVNNVATAAQVYKAISTGMPLIERTVTITGRGIKDPKNLRIRF
                     GTLVSDVLEECGGLSCEPGKIIVGGPMMGMAQHTMEIPFTKGISGIVFMPPEEVNEDE
                     MLPCIRCGKCIQACPIQLMPLNISAYALKGDYDTCETLHALDCVECGSCSYICPSRRP
                     LQQSIKVAKEAIIAARKNEN"
     gene            831..1796
                     /locus_tag="VFD89_09995"
     CDS             831..1796
                     /locus_tag="VFD89_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HZJ58547.1"
                     /translation="MSNTLIVSASPHIRSKESVSRIMLDVILALIPAAIAAVIVFGWR
                     VLLVIGVSVIGAVITEAILQKLMGKPVTIKDLSAVVTGILLAYNMPPTIPLWMVMIGS
                     MIAIALVKQVFGGLGQNFMNPALAARAILFAAWPVQMTNWQRPIIDALSNATSSATPL
                     PILKDGEAWAKLPPLWDVFVGKIGGSIGEISALALLLGAAYLLLRGVINWRIPVTYIA
                     TVALLTWVFGTDGFFTGRGIYHIFSGGLILGAFFMATDYPTSPVTPHGQIIMGVGCGI
                     LTSVIRLVGGYPGGVSYSILIMNVATPLIERYTKPRIYGEVKSNV"
     gene            1789..2613
                     /locus_tag="VFD89_10000"
     CDS             1789..2613
                     /locus_tag="VFD89_10000"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZJ58548.1"
                     /translation="MSDIMKIGLKLFVITVIAAIVMGLTNLATEEPIRVQRVKAENQA
                     RQATLPQAKDFNQLDIKEGTHGNENAEIVEIYSGISDDSIVGYVFRVRAKGYAGDIDI
                     ILGINTNHEIEAVEIGEHNETPGLGAKAKDKTFLAQFHGKETEAQLSAVKTLSGDGSV
                     DASGSSTVDAEGSATSDTDSSATADEDGSATSDADGSTTADTEDSATTDADSGATTDT
                     DSSATVDADSGATQVSGSVQEVQAITGATVTSSAVTRAINLALQYYNQILLNGGGN"
     gene            2613..3206
                     /locus_tag="VFD89_10005"
     CDS             2613..3206
                     /locus_tag="VFD89_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HZJ58549.1"
                     /translation="MKASKAIINGIITENPIFRLVRGMCPTLAVTTAAINGIGMGLAT
                     AFVLIASNLVISLIKDFIPSQVRIPAFIVVAATFVTVTGMLMEAFIPGLYESLGIFIP
                     LIVVNCIILGRAEAFASKNSPLVAVADGIGMGLGFTLALTLLGIIREILGAGTLFGMQ
                     LMPQAFNPAVIMILPPGAFLTLGLLLGLLNKLTAKTK"
     gene            3220..3807
                     /gene="rsxA"
                     /locus_tag="VFD89_10010"
     CDS             3220..3807
                     /gene="rsxA"
                     /locus_tag="VFD89_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HZJ58550.1"
                     /translation="MGYTGKLFLILLSTVLVNNFVLSRFLGICPFLGVSKKVSTAMGM
                     GAAVIFVMALASLFTYIIQYAILEPLSLEYLQTIAFILVIAALVQFVEMVIQKMSPTL
                     YQALGVYLPLITTNCAVLGVAIINIREDYDLLQTLVNGTGAALGFTLALVLMAGIRER
                     LELSDIPESLKGFPITLITAGLMSVAFLGFQGLIK"
     gene            3818..4153
                     /locus_tag="VFD89_10015"
     CDS             3818..4153
                     /locus_tag="VFD89_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HZJ58551.1"
                     /translation="MLIDILMPVLSLGGMSLLFGAGLAFASHKFAIETDPREEQVREV
                     LPGANCGACGYPGCDGFAAAVAAEQAPITGCTVGGPNVAQDIGFIMGLEAGETQRKNR
                     KGSLRRGLR"
     gene            4059..4649
                     /locus_tag="VFD89_10020"
     CDS             4059..4649
                     /locus_tag="VFD89_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HZJ58552.1"
                     /translation="MSPRISASLWAWKQEKHSERTAKVLCAGDCDRATDKYHYEGIED
                     CVAASMLSDGPKECSYGCLGLGTCVRACPFDAIYIDKGIAVVDEEKCTGCEICVAACP
                     KHIIEMIPASSQVQVMCKSEDKGRDVRKYCTVGCIACRICERACEYDAIKVENNIARI
                     DYDKCVNCMVCAEKCPTKSIYADFENRRPEFVGMKN"
     gene            4655..5965
                     /locus_tag="VFD89_10025"
     CDS             4655..5965
                     /locus_tag="VFD89_10025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZJ58553.1"
                     /translation="MNNYPSGEYHQGEDVAPGPPVGSRGTFYWADVREGLLDIAKGYE
                     GKVQLVYMDPPFMTGNQFKYRQKAGEKGFVEHIAYSDQWVGGKEYYLNFMKRVFEHVY
                     DLLTPEGSFYLHVDYRTSAYLRIILDGIFGEENFLNEIIWHYRSGGRARRHFSRKHDN
                     ILFYRKSSEHFFNIEAVGQERGTEKRNNMKREVDHKGRVFWSIKSGGKVYRYYEDSKI
                     YPSDVWTDISHLHQRDPERIGYDTQKPEALLERIILSSSRPGDLVADFFAGSGTSLSV
                     AAKLGRKWLGMDSSPHSMHVCRKRLLDLDTGRDFAIYYGGGIGPADKAKLVAERYENG
                     FSGDDNASEGSNIIKITDYMPADINKIQIQGNVSRALRGLEYIDYWAVGYIKGNLYYP
                     IKWARRMDGQSVVNNSIVLNGSVDQDMVIHVVDIAGDQHFYKFT"
     gene            complement(6096..7013)
                     /locus_tag="VFD89_10030"
     CDS             complement(6096..7013)
                     /locus_tag="VFD89_10030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HZJ58554.1"
                     /translation="MKKINIGNGAIQASEISLGCMRISSLSLKEAAHLINTALEAGID
                     FFDHADIYEGGKCEETFAQAIDMNPSIRERFIIQTKCGIRKGFFDFSKEHILSAVDGS
                     LKRLQTDYIDVLLLHRPDALVEPEEVAEAFTALYNSGKVKYFGVSNQNPMQIELLKKY
                     LNHKIIINQLQLSITNTGMIDAGLNVNMEIDPSINRDGSILDYCRLNDITIQPWSPFQ
                     YGYFEGVFLDNDKFPELNSKIDEIAENRGVTNTAIAVAWLLRHPARMQPIVGTTNPDR
                     LKDISKASGVNLTREEWYAIYRAAGNELP"
BASE COUNT         1893 a         1365 c         1869 g         1945 t
ORIGIN      
        1 atcgaagata ataagccgga tgccattgca gccatggagg aggcaatcac ggatgaacct
       61 aatattacca ttaagaaatt aagaactaaa tatcctcagg gttgcgaacg caaattaatt
      121 gaaacgataa caggtagaca aataccgtcg ggcgggttac caatggatgt cggcgccata
      181 gtaaacaacg tggcaactgc tgctcaagtt tacaaagcga tcagcacggg aatgccgttg
      241 atagagagaa cagttaccat taccggaagg gggataaagg atcccaaaaa tcttcggata
      301 aggtttggta ccctggtaag cgatgtgtta gaggaatgtg ggggtttatc ctgcgagccc
      361 ggcaaaataa tcgttggtgg gcctatgatg gggatggcac agcatactat ggagataccc
      421 tttacaaagg ggatttccgg aattgttttt atgccgccgg aagaggttaa cgaggacgag
      481 atgctgccgt gcattcggtg cgggaaatgc atccaagcct gccctattca actgatgccg
      541 ctgaacatca gcgcttatgc attaaaaggt gattatgata cctgcgagac ccttcatgcg
      601 ctagattgcg tagaatgcgg ctcctgttca tacatttgcc cctctagaag gccactgcag
      661 cagtctatca aggtagccaa ggaggcgatt attgctgcga gaaaaaatga gaattagtcg
      721 ggtatatgat tttaattttg gacacggttg atttaacatt ttgaaactca attgttgggt
      781 tgttgttcaa tggagcctaa tatctttgta ataggtttgg agggaaagtg atgagcaata
      841 cattaatagt ttctgcatcc ccccatatca gatctaagga gtcggtatca aggataatgt
      901 tggatgtaat cttggcattg attcccgcgg caattgctgc agtgatcgtt tttgggtgga
      961 gggtgttatt agtcattggt gtatccgtca taggggcggt aatcaccgag gcaatattac
     1021 aaaaactaat ggggaaaccc gtgactatta aggatttaag tgcggtggta acaggcattt
     1081 tgttggcata taatatgccg ccaacaattc ctctatggat ggtcatgatc ggctcgatga
     1141 tagctattgc cctggtaaaa caggtatttg gcggattggg ccaaaatttc atgaaccccg
     1201 ctctggccgc ccgggctata ttatttgcgg cttggccggt gcagatgaca aattggcaaa
     1261 gaccgattat agatgcttta agtaatgcca ccagcagtgc aaccccgctg cccatattga
     1321 aggatggtga ggcatgggca aaattaccgc ccctatggga tgtctttgtg ggaaagatcg
     1381 gggggagcat cggtgagata tctgccttgg ctctattgtt gggagcggct tatttgctct
     1441 taaggggtgt tataaattgg agaatccccg taacgtatat tgctactgtc gctctgttga
     1501 catgggtatt tggaacagac ggttttttca caggccgggg tatataccat atcttttcgg
     1561 gtggccttat attaggtgcg ttttttatgg ctaccgatta tcccacttcg cctgttaccc
     1621 ctcatggtca gattatcatg ggcgttggtt gcggtatctt gacatcggtg attcgtctgg
     1681 tggggggata tccgggtggg gtatcttatt ccattctgat tatgaacgta gctacacctc
     1741 ttatagaaag atatacaaaa ccccgaatat atggggaggt gaaaagcaat gtctgatata
     1801 atgaaaatcg gattgaaact ctttgtgatt acggtgatcg cggccatagt tatgggcctt
     1861 accaatcttg ccactgaaga acccataagg gtgcagcggg tgaaggctga aaaccaggca
     1921 aggcaagcca cgcttcccca ggcaaaggat tttaatcaat tggatataaa agagggaacc
     1981 catggaaatg aaaatgcgga aatagtagaa atatactccg gaatatcgga tgatagtatc
     2041 gtaggttatg tttttagggt aagggctaag ggttatgcag gggacataga tatcatcctg
     2101 ggtataaata caaatcatga gatagaagct gttgagatag gtgaacacaa tgaaactccg
     2161 ggtttaggtg ctaaagcaaa ggataagacc tttttagcgc agtttcacgg aaaggaaaca
     2221 gaggctcagc tttctgcggt taagactttg agcggtgatg gaagcgtaga tgccagcggc
     2281 agctctactg tagatgcgga aggcagtgca acctctgata cggacagcag tgcgactgct
     2341 gatgaagatg gcagcgctac ttctgacgca gatggcagca caactgcgga taccgaagat
     2401 agcgcaacca ctgacgcaga cagcggcgca actaccgata cagacagcag tgccactgta
     2461 gacgcagaca gcggcgccac acaggtttca ggcagcgtac aagaagttca ggctattaca
     2521 ggtgctactg tgacctcaag cgctgttacc agagccatta atctggccct acagtattat
     2581 aatcaaattt tactaaatgg gggtgggaat taatgaaggc atcaaaggca atcattaatg
     2641 gaataatcac cgaaaatcca atctttagat tggtgcgggg gatgtgccct accttggccg
     2701 ttactacggc tgccataaac gggataggca tggggcttgc cacggctttt gttcttatag
     2761 cctctaacct ggtaatatcc cttataaagg attttatccc gtcacaggtg aggatcccgg
     2821 catttatcgt cgtggcggca acttttgtta ccgtaaccgg tatgctcatg gaggccttta
     2881 ttccgggatt atacgagagt ttggggattt ttattcccct aatagtggtt aattgtatta
     2941 tccttggcag ggcagaggcc tttgcatcaa agaattcacc cttagtagcg gtagcagacg
     3001 gaataggaat gggtctgggg ttcacccttg ccttgactct cttggggatt atcagagaga
     3061 ttttaggtgc ggggaccctg ttcggtatgc agcttatgcc ccaggccttt aatccagcgg
     3121 ttattatgat cttaccgcct ggtgcatttc tgaccttggg gttgttgctt ggactgttaa
     3181 ataaactgac cgccaaaacc aaataagggg ggattggaca tgggatatac aggaaagttg
     3241 tttttgatat tattgagtac ggtattggta aataatttcg tattgtctag atttttgggg
     3301 atatgcccct ttcttggagt atcgaaaaag gtatcgacag ccatgggtat gggtgcagcg
     3361 gtaatattcg taatggccct agcttctctg tttacctata taatacaata cgcaatctta
     3421 gagcctttaa gtcttgagta tcttcagacc attgctttta tactggttat tgctgcttta
     3481 gttcaatttg tagagatggt tatccaaaag atgagcccaa ccctatacca ggcccttggc
     3541 gtatatcttc cccttataac caccaactgt gcagttttag gggttgctat aataaacatt
     3601 cgagaagatt atgatttact tcagaccttg gttaacggga ccggtgcggc actgggcttt
     3661 accttagcct tggtgttgat ggcggggatt agggaaaggt tggaactctc tgacataccc
     3721 gaatccttga agggctttcc cattactttg ataactgcag gactgatgtc agtggcattc
     3781 ttgggatttc aagggcttat aaagtagggg ggatattatg ctgattgata tattgatgcc
     3841 tgttctcagc cttggaggga tgagcttgtt atttggggcg gggttagcct ttgcgtccca
     3901 taaatttgct attgagactg atcctaggga agagcaggtg agggaagttt taccgggagc
     3961 gaattgtggc gcctgcggct atcccggatg tgacggattt gctgcagctg tagctgcaga
     4021 acaggctccc ataacgggct gtaccgtagg cgggccaaat gtcgcccagg atatcggctt
     4081 cattatgggc ctggaagcag gagaaacaca gcgaaagaac cgcaaaggtt ctctgcgccg
     4141 gggattgcga tagggcaact gataaatatc actacgaagg tatcgaagac tgtgttgcgg
     4201 catccatgtt atccgacgga cctaaggaat gtagctatgg atgcttgggg ctcggaactt
     4261 gcgttagggc atgccctttt gatgcgatat atatagacaa ggggattgcc gtcgtggatg
     4321 aagaaaaatg taccggttgc gagatatgcg ttgctgcctg tccaaaacat attattgaga
     4381 tgattcctgc atccagccag gtccaggtta tgtgcaaatc tgaggacaaa ggcagggatg
     4441 taagaaagta ttgtacggtt gggtgtatag cgtgtaggat ttgtgagaga gcatgcgaat
     4501 atgacgctat aaaggttgaa aataacatag cacgaattga ttatgataaa tgtgtcaact
     4561 gtatggtttg tgcagaaaag tgtcccacca agtccatata tgccgatttt gagaataggc
     4621 gtccggaatt tgtagggatg aagaattgat tattttgaat aactatccgt ccggtgaata
     4681 tcaccaagga gaggatgtag caccggggcc cccagttggg tcccggggta cattctattg
     4741 ggctgatgtc cgggaaggcc tgttagatat agctaaaggg tatgaaggaa aggtgcaact
     4801 ggtctatatg gatccgccct ttatgacggg gaaccaattt aaatatcgtc aaaaagcagg
     4861 cgaaaagggt tttgtagagc acatagccta cagtgatcaa tgggtcggcg gaaaagaata
     4921 ttatctaaat tttatgaagc gagtttttga acatgtgtac gaccttctca ccccggaggg
     4981 ttctttttat ctccatgtgg attataggac atctgcttac ctgaggatta tattggacgg
     5041 gatattcggg gaagagaact ttcttaacga aataatctgg cattaccgaa gcgggggcag
     5101 ggcaaggaga cacttcagcc gcaaacacga taacatcctt ttttaccgaa aatcttcgga
     5161 acatttcttt aatatagagg ccgtgggaca agagcggggt accgagaagc gcaacaatat
     5221 gaaaagggaa gtggaccata agggcagagt gttttggtcc attaaatccg ggggaaaggt
     5281 atataggtat tacgaggatt ctaagatata ccccagcgat gtatggacgg acatttccca
     5341 cctgcatcaa cgggatccgg agcggatagg atatgatacc caaaagcccg aagccttgct
     5401 ggaaagaatt atactatcct cttccaggcc gggggacctg gtggcggatt tttttgcagg
     5461 ttccggcacc agtctatcgg ttgccgccaa gctgggtcgt aaatggttgg gtatggattc
     5521 cagtccccac tccatgcacg tctgcagaaa aaggctgttg gacttggata cgggacgtga
     5581 ttttgctata tattacggtg gcggtattgg gcctgcagat aaggcaaagt tggttgcgga
     5641 aaggtacgaa aatggatttt ctggggatga caatgcttcc gaaggctcaa atatcataaa
     5701 aataacggat tatatgcctg cggacattaa caagattcaa atccaaggga atgtgagcag
     5761 ggctttaagg ggcctggagt atatagatta ttgggcggtt ggatatataa aaggcaactt
     5821 atattatccg ataaaatggg caaggagaat ggacggacaa tcggttgtca ataattctat
     5881 tgttttaaac ggctctgtgg atcaagatat ggtgatccat gtggtagata tcgccggtga
     5941 tcagcatttt tataagttta cataacctat taaacggggc agtatgattg gttgaaacta
     6001 tggtctttga gatgcggtag gatcagatga tattaattgc accgataaat aatgggctac
     6061 aggaaaggct gtagcccatt atttgtcggt gtgtattatg gcaactcgtt tccagcggcc
     6121 ctatatatag cgtaccattc ttctcgggtg agattgaccc ctgacgcctt agagatgtcc
     6181 tttagccgat ccgggttggt tgtgcctacg atgggctgca ttctggcggg atgccttagg
     6241 agccacgcta ccgcaatcgc cgtattggtt acgcccctat tttctgcaat ttcatcaatt
     6301 ttggaattga gttcggggaa tttatcgttg tctaggaaaa cgccctcaaa gtatccatat
     6361 tggaaggggg accaaggttg gatggtgatg tcatttaggc gacaataatc caatatgctg
     6421 ccgtccctat tgatggatgg gtcaatttcc atattaacat ttagccccgc atctatcatg
     6481 ccggtatttg tgatgctgag ctggagctgg ttaataataa ttttatggtt taaatatttt
     6541 ttaagtagct ctatctgcat tggattttgg ttgctgaccc caaagtattt aaccttaccg
     6601 ctattataca gggctgtaaa ggcttccgct acttcctcgg gttcaaccag agcatcggga
     6661 cgatggagca gtaagacatc gatataatcc gtttgcaggc gcttgagact gccatcaact
     6721 gcggacagga tgtgttcctt ggagaaatca aaaaagcctt tcctaatacc acatttggtt
     6781 tggataataa accgttcacg aatgctggga ttcatgtcaa tggcctgggc gaaggtctct
     6841 tcgcatttgc cgccctcgta tatatccgca tggtcgaaaa aatcgattcc cgcttccagt
     6901 gcggtgttga ttagatgtgc ggcctctttt agggaaaggc tggaaatcct catgcacccc
     6961 aaagaaattt ctgatgcctg gatcgcaccg ttgccaatat tgattttttt catggattac
     7021 accactttca tatttatttt tataccctaa tatataggat gtcctataaa tg
//
LOCUS       DASRNX010000251         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_582607,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000251 DASRNX010000000
VERSION     DASRNX010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582607"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VFD89_10035"
     CDS             <1..1153
                     /locus_tag="VFD89_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HZJ58555.1"
                     /translation="GEMIKDLRRAAEEPNGDFVVANYNSDAPTQVLKPISIDNHREAG
                     GAVSESVAGDNNPKKRPWIKAIVMVLIFAMIMGLAFYIGMRIYKNNFTSQEIEVPGII
                     NQTFEDAHRHLREKSLYLKKDGEAFDDDVEAGHIISQDPEEGTKVKPDFTVKVILSLG
                     PETANVPNVEQRTIREAEIILENESFIIGEHQYINSEFPSDTVIGQSLEADTQVPKGS
                     EIWLTISLGPEETVAIVGNYVGQQLDVAKQMIEGDKLKFGKVKEERYHDTFSKGIVIE
                     QSLKHGDTVSEYRVIDLVLSLGPQPTYPKDISIDLSSITDRETVNIVVKKTQNSKTTE
                     VYNRFHTVSDEELIIPLVGTGVAEYKIYIDDELAFERIVDFTKKGGDAG"
     gene            1153..2025
                     /gene="rsgA"
                     /locus_tag="VFD89_10040"
     CDS             1153..2025
                     /gene="rsgA"
                     /locus_tag="VFD89_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZJ58556.1"
                     /translation="MKKGIITRGVGGFYDVLVEDRAIRCRARGIFRKDNIVPMVGDNV
                     IISVSDRTILEILPRKNQLLRPAVSNIDILGIVIAAVHPLPDYYLVDKLMVNAQYNEV
                     ETVLIINKTDLAGDSAIEDIFDSYRSTGYPIMALSCKEGVGFEQFREIIKGKVVSLAG
                     QSGVGKSSIINILYPEKQLEIGDLSDRISRGRHTTRHTKLLTLPSGAMIVDTPGFSTM
                     NMDELEPEELAYLYPDFLEYIHDCRFSGCVHDQEPGCRIKGAVSEGTISEGRYKRYIR
                     ILNELREFRRNIWG"
     gene            2016..2678
                     /gene="rpe"
                     /locus_tag="VFD89_10045"
     CDS             2016..2678
                     /gene="rpe"
                     /locus_tag="VFD89_10045"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010172372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZJ58557.1"
                     /translation="MGIKIAPSILAADFSRLGEQVKMLDDGGADYIHIDVMDGHFVPN
                     LTFGPVVIKAIRDRTHLPFDVHLMMNNPLEYLNDFVDAGADIITVHAEVLPHLHGAVQ
                     QIKRMGVKAAVSLNPSTPLHVLDYVMEDLDMVLLMSVNPGFGGQQFIPATMDKIRELN
                     RKIDASGLEIDIQVDGGISIDNIREISLAGANVFVAGSAIFNSCNPKEMIKRMRRKAE
                     ER"
BASE COUNT          863 a          512 c          710 g          694 t
ORIGIN      
        1 gggagaaatg attaaggatt tgagacgggc tgcagaggaa cccaacggtg actttgttgt
       61 tgccaattat aacagcgatg ctccgactca ggtattgaaa cccattagca tagataacca
      121 tagggaagcc gggggtgcgg tatctgaatc cgtagccggt gataataatc ccaagaaaag
      181 gccttggatt aaggctatcg ttatggtcct catttttgct atgataatgg gtttagcgtt
      241 ctatattggt atgcgtatat ataaaaacaa cttcacatcc caagagatag aggttcccgg
      301 gataatcaat caaacctttg aagacgcaca caggcactta cgggaaaaaa gtttatacct
      361 aaaaaaggat ggagaagctt ttgatgacga cgtggaggcg ggccatataa tttcccagga
      421 cccggaggag ggtactaagg tcaagcccga ttttacggtt aaggtaattc tgagcctagg
      481 accggaaact gccaatgttc ctaatgttga acaaaggacc atccgcgaag cggagataat
      541 ccttgaaaat gaaagtttta taattggcga gcaccagtat ataaacagcg aatttcccag
      601 tgacaccgtc ataggtcaat ccctggaggc tgatacacag gtccccaaag gttcggaaat
      661 ctggttgacc ataagtctgg gcccggaaga aacagtcgca attgtaggaa attatgtcgg
      721 ccaacaactg gatgtggcaa agcagatgat cgagggggat aagctgaaat tcggaaaggt
      781 gaaggaggaa aggtatcatg ataccttcag caaaggtata gtaattgagc aaagcctaaa
      841 gcatggtgat accgtatcgg aatatagggt aatcgacctt gttctaagcc ttggaccgca
      901 acctacctat cctaaggata taagcatcga tctctccagc atcaccgata gggagactgt
      961 aaatatagtc gttaaaaaaa cccaaaacag caagaccacc gaagtctata atagatttca
     1021 cacggtgagc gatgaagagt tgatcattcc gctggtgggc accggtgtag cagaatacaa
     1081 aatttatatt gatgatgaat tagcctttga aaggattgtg gatttcacaa aaaaaggggg
     1141 agatgcaggt taatgaaaaa aggaataata acaagagggg taggtggctt ctatgacgtt
     1201 ttggtcgaag atagggccat tcgctgcagg gcaagaggca tattcaggaa ggataatatc
     1261 gtaccgatgg tgggggataa cgtcataata tcagtatctg acaggaccat tctggagata
     1321 cttccccgaa agaatcagct ccttcggccg gccgtatcca acattgatat tttggggatt
     1381 gtcattgcgg ccgttcatcc tttgcctgat tactacttgg ttgataagct catggtcaat
     1441 gcccaataca acgaagtgga gacggtatta ataataaaca aaacggacct ggcaggagat
     1501 agtgccatag aagatatctt tgattcctat aggtctacgg gctatcctat tatggctctg
     1561 tcttgcaagg aaggagtcgg atttgagcag tttcgagaaa ttattaaggg aaaggttgtc
     1621 agcctggcag gccaatcggg agttgggaaa tcctccatca taaacatcct ctatcccgag
     1681 aagcagttgg aaatcggcga tctcagcgat aggattagca ggggtaggca taccacccgc
     1741 catactaaac tgttgacttt gccttcaggg gctatgatag ttgatacccc cggattcagc
     1801 actatgaata tggatgaact ggaacctgaa gagctggcat atctatatcc ggactttttg
     1861 gaatatattc atgattgtag gtttagcggc tgcgtccacg accaagaacc cggatgcagg
     1921 ataaaggggg ccgtatcgga aggaacgata tctgagggac gctacaaaag atacataaga
     1981 atacttaatg aattaaggga gttcaggagg aatatatggg gataaagata gcaccgtcaa
     2041 ttcttgcagc tgatttttcc agactgggcg agcaagtgaa gatgttggac gacggagggg
     2101 ccgactatat ccatatcgat gttatggatg ggcattttgt gccgaatcta acatttggtc
     2161 ctgttgtaat aaaggcaatc agggacagaa cccatcttcc cttcgatgta caccttatga
     2221 tgaataaccc attggaatat ttgaatgact tcgtagatgc cggggctgat ataataactg
     2281 tacatgccga agtattgcca catttacatg gggccgttca acaaattaaa cgtatgggtg
     2341 ttaaggcggc ggtatcctta aatccatcaa ccccattaca tgtcttggac tatgtaatgg
     2401 aagatttgga tatggttttg ctgatgagtg ttaatcccgg gttcggggga cagcaattta
     2461 ttccggcaac catggataag attagagagc tcaacagaaa gattgacgca tcgggactgg
     2521 aaattgatat tcaggtggac gggggtatat ccattgataa tataagggaa ataagcctgg
     2581 ccggagcgaa tgtttttgtg gctgggtcag ctatattcaa ttcctgtaac ccaaaggaaa
     2641 tgattaaaag gatgagaagg aaagcagaag aaagataacg atagataatg cgcttattac
     2701 acagggagca attcgtctag ttgagcgcca ttgtcacaca tgatgtgata aatgcatata
     2761 tataaaatat gtattatgc
//
LOCUS       DASRNX010000252         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_104285,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000252 DASRNX010000000
VERSION     DASRNX010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104285"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /gene="fliD"
                     /locus_tag="VFD89_10050"
     CDS             <1..1756
                     /gene="fliD"
                     /locus_tag="VFD89_10050"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HZJ58558.1"
                     /translation="LYSKTNMTSQSAYNILKVQSSDEKYVTATATTGAYVSDRTISKI
                     EMAKAAQLEGTSVSAPLQSGELTGDINLVGLHRITVTLDGTTKVIEFDVKENQIQGQD
                     GLLEAFQGGLDKAFGMDRINVAFEGNKIELNAGNSVIRVLEHPTEDGVDSLGFSPNQS
                     NRINLDSTLWGMWEFEMTGTPLEFDSEGMINFTINNVDFSFEGNDTLRTVINRINSSN
                     AGVTIAYSTLTDRFQLTNKDTGAAHTLTFRDGLDEVYESGEAKGGNFLEALGFAPLDP
                     DHPEGVPKGRDALMVMDSITIYRSTNNFTIDGVTYNLTQNFEAVEGQEPITLTMSTDV
                     DEAFDNIKGFVDAYNKIIDTINRSLSQERFKDFLPLTDEQREAMGEKEVQLWEEKARS
                     GLLQRDPLLQKIVFDMRRALVDSVEGMDINLSSIGITTGSYTEAGRLHIDEGRLKNAL
                     REKGDDIMRLFTSKSEIVYSPDNDSNERQTRYSESGLVNRLSDILQDNIRTRRDANGR
                     KGILLEKAGIVGDSSERVNLVDDQLKDMNKRIDAALERMYRAENRYWAQFTALETALQ
                     QMNSQSMWLMQQFGGGMY"
     gene            1928..2317
                     /gene="fliS"
                     /locus_tag="VFD89_10055"
     CDS             1928..2317
                     /gene="fliS"
                     /locus_tag="VFD89_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HZJ58559.1"
                     /translation="MAIANPYQKYQQQSVMTATPGELTLMLYNGCIRFIKQAQLGIEA
                     GDIEKAGNSILRCQDIIEELMITLRPDYEISNSLSALYDYIYNQLVDANISKDASKLN
                     EALGLVTELRDTWAEAIKINRRHVYGG"
     gene            2320..2829
                     /gene="flgN"
                     /locus_tag="VFD89_10060"
     CDS             2320..2829
                     /gene="flgN"
                     /locus_tag="VFD89_10060"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HZJ58560.1"
                     /translation="MDGGQGKNKGELYGNLKELIVITSDKRHRLYSLLKMTQMQRTAI
                     EEMDLDLLAKYLGEKQRHIDAIDALDVRFTEIYGSDIKAGLADGGLGQNDPNVRGLYA
                     QLQTLISDVQEIIKAIHSQEESNNTKAQEAMEDLKQKIGHIQTGKKGHSAYTQTHDIS
                     EGMFIDQKK"
BASE COUNT          988 a          562 c          774 g          681 t
ORIGIN      
        1 cctctactcc aagaccaata tgacctcaca aagtgcctat aacatactaa aggtacaatc
       61 cagtgatgag aaatacgtaa ccgctacagc tactacaggc gcatatgtat ccgacagaac
      121 catttcgaag atagagatgg cgaaggctgc ccagcttgaa ggtacatcgg tctcggcccc
      181 tttacagagc ggggaactga ccggggatat aaatttggtg ggtcttcaca gaataactgt
      241 cactttagac ggcactacca aggttataga atttgatgtt aaagaaaatc agatccaagg
      301 ccaagatggc ttgctagagg cttttcaggg tggactggac aaagcttttg ggatggatag
      361 gataaacgta gcatttgagg gaaacaagat agaattaaat gcaggaaata gtgtcataag
      421 ggttttagag cacccgacag aagacggtgt ggacagtttg ggttttagcc ccaatcagag
      481 taaccgtatt aatttagatt ccaccctttg ggggatgtgg gaatttgaga tgacgggaac
      541 ccccctggaa tttgattccg agggcatgat aaattttact ataaacaacg tagattttag
      601 ttttgaggga aatgacaccc tgcggacggt tataaaccga ataaacagca gcaacgccgg
      661 tgtaaccatt gcctatagca cactcacgga tagatttcag ctcaccaata aggatacagg
      721 agctgctcat accctgacat ttcgggatgg attggacgaa gtctatgaga gcggtgaggc
      781 caaagggggg aatttcctag aggctctggg atttgcaccc ttagaccctg atcatccgga
      841 aggagttcca aagggccggg atgctctaat ggtcatggac agcataacca tatacaggag
      901 cactaataac tttacaatag acggagttac ctataaccta actcagaact ttgaggccgt
      961 agaagggcaa gagcctataa ccctgaccat gagtaccgat gtagatgaag cttttgataa
     1021 tataaaggga tttgtagatg cctataacaa gattatagat accataaata ggagtctgtc
     1081 ccaggaacgt tttaaggatt ttctgccgtt gacggacgag cagcgggaag ctatgggtga
     1141 aaaggaagtc cagctctggg aagaaaaagc ccgaagcggc ctattgcaaa gggatccgtt
     1201 actacaaaaa atagtctttg atatgcggag ggccttggta gattccgtag agggtatgga
     1261 cataaaccta tcttccatag gaatcaccac gggatcctat accgaagccg gaagactgca
     1321 tatagacgag ggtagactaa aaaatgccct gagggaaaag ggcgacgata taatgcgtct
     1381 ttttacaagc aagtcggaaa tagtctattc ccccgacaat gacagtaacg aaaggcagac
     1441 caggtatagt gaatccggat tggttaatag gctttccgac atccttcagg acaatatacg
     1501 tacccgaagg gatgcaaacg gtagaaaggg tattctccta gaaaaggcgg gtatagtagg
     1561 ggattcatcc gaaagggtaa accttgtaga tgatcagcta aaggatatga acaagcgcat
     1621 cgatgccgcc cttgaaagaa tgtacagggc agagaacagg tattgggcgc aatttaccgc
     1681 cttggaaacg gctcttcagc agatgaactc tcagagcatg tggctgatgc aacaattcgg
     1741 gggcgggatg tactgattgg agtttggagg ctagaagtta gaggttggat tctgatagtt
     1801 agaactttaa aatcaggagc tatgaagtta ggaattgggg attagaaatt ggaaattgga
     1861 gttgggcgat gggatatact gctttttatc ttgaacaaga ggttaatttg caaggaggca
     1921 actaacaatg gcaatagcaa acccatacca aaagtatcaa cagcaaagtg taatgacggc
     1981 taccccaggg gaactgacgt taatgctata taacggctgc ataaggttta taaagcaggc
     2041 acagctagga attgaagccg gggacataga aaaggcggga aactccatct taaggtgcca
     2101 ggatatcata gaggagctga tgattacact aaggccggac tatgagatct caaattctct
     2161 atcggctttg tatgattaca tatataatca attggtggat gctaatatat ccaaggacgc
     2221 atccaagctc aatgaagcct tggggctggt tacagagctt agggacacct gggccgaagc
     2281 catcaagata aacagaagac atgtctacgg aggataaaga tggacggggg acaggggaaa
     2341 aacaaagggg agttgtacgg gaacttaaaa gagttgattg ttattacctc ggataaaagg
     2401 catagactct atagcctttt aaagatgaca cagatgcaaa ggacagccat agaggagatg
     2461 gatctggatt tactggcaaa gtatctgggg gaaaaacaaa ggcatataga cgccatagac
     2521 gccctggatg tgcgatttac cgaaatttat gggtcggata ttaaagctgg gttggcggac
     2581 ggtgggcttg ggcaaaatga tcccaatgta aggggattat acgcacagct acaaaccctg
     2641 atttcggatg tccaagagat catcaaggct atacacagcc aggaagaaag caacaacacc
     2701 aaggcacaag aggccatgga ggatctgaaa caaaagatag gccacattca aacgggtaaa
     2761 aagggccata gcgcctatac ccaaacccat gatataagcg aaggcatgtt catagatcaa
     2821 aaaaagtagc cctttggatg ccatataagt atccaaaggg tcttattatg ccgatatagg
     2881 acgtcctgag aaatattatg cccgcccact taataaatca ataaaacaaa gattcatcct
     2941 attcccaata gatttaagta ctaaaaagaa tttaataaaa atatgacgag tggagggttt
     3001 gggag
//
LOCUS       DASRNX010000253         3381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_477719,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000253 DASRNX010000000
VERSION     DASRNX010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477719"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1653)
                     /locus_tag="VFD89_10065"
     CDS             complement(166..1653)
                     /locus_tag="VFD89_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZJ58561.1"
                     /translation="MWINKKAFYSLRWRIAIAYLILIGIGFVVTNLSIIKVFENRQIH
                     DKQERFRAYAVQVAQVISKDYSGSNTEMKENIIFDISQLGEDIMYREGGQPTRILILD
                     ANGIVDFDSYNDLSDHGFLKRNLRNDFPIIDDLLAGNDVDPTALHIGDRFSDQKWVMY
                     SYSPIIYESQKVTGAVILSTSLSDIGEILEAIGAMLYRSSLVIIAFVILISIGISAYI
                     TRPIKSLTDVIRKMGQGHLGQRVKVRSVGEFRELGEAFNIMSEKLENLDRARSEFVSN
                     ASHELKTPLSAIKVLAESLIHMGGEVPEIYIEFLGDINKEIDRLNAVITDLLSLVEID
                     AHDKMGEKKVVDLARLVVRTVGGLRVLAEGKNISLETAIGSGSTTGNVVRLQQAVSNL
                     VDNAIKYTPEGGRVSVDLYREEDQAIIKISDTGIGIPPEDIPHIFDRFFRVDKARSRY
                     TGGTGLGLSITHRIILLHGGSIKVDSKEGRGTTFSISLPLWEEEG"
     gene            complement(1640..2329)
                     /locus_tag="VFD89_10070"
     CDS             complement(1640..2329)
                     /locus_tag="VFD89_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZJ58562.1"
                     /translation="MQKNLLIVDDEPAIIKGLKFSLEQDGYSTVEAYDGQEAVDKFFG
                     EEFDLVVLDVMLPGLNGFEVLQRIREKSSVPVIMLTAKGEDMDKILGLEYGADDYITK
                     PFNILELKARIKAIFRRSNHSEGRNQEQVIDIHNITINIANRSVIKDGEDVNLTAKEF
                     DLLQLFASNPGKVYSRESLLETVWKYDYLGDLRTVDVHIRRLREKIETNPSQPEFIFT
                     KWGVGYYFVDK"
     gene            complement(2509..3165)
                     /locus_tag="VFD89_10075"
     CDS             complement(2509..3165)
                     /locus_tag="VFD89_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZJ58563.1"
                     /translation="MITIIQIQNKHIKFIAIVGIIMIGTLSSIIFKDSIFHPGKPGGV
                     EVVPRETSSEVESRDGINGGQQEDQSEPQMPNIIKIYITGQVRNPGVIEVPAESRLIE
                     AAEMAGGFLDEADLLKINLALRVQDEGMYIIPKIGEEGPEINSAGAIVNEGDSKIDIN
                     TADQKQLETLPRIGPVIAGNIINYREQNGIFKTEEDLMNVPGIGQKTFDGLKDFIRIK
                     "
BASE COUNT          865 a          844 c          611 g         1061 t
ORIGIN      
        1 tacaaccggc aattatagta accagtatca tcaacaggat tatatgccaa agcttcttac
       61 ccaatgttcc acctccggtt cttattgggc tatttacgca tcttcattat aggggattat
      121 aattacaaaa ggtttaagca aggttaacat tttaataaaa catatctatc cctcttcttc
      181 ccataggggc agactgatag aaaatgtggt tcccctgcct tccttgctat ccacctttat
      241 acttccgccg tgtaacagta ttatcctatg ggtaatggac aggcccaacc cggttcctcc
      301 ggtatatctg gatctggctt tatctaccct aaaaaaacga tcaaatatat ggggaatatc
      361 ttcaggaggg ataccgattc ctgtatcgga aattttgatg atggcttgat cttcctctct
      421 gtataggtcc acagaaactc gaccgccctc aggtgtatat tttatagcat tatccaccag
      481 attgctgacg gcctgttgca atcttacgac atttcccgtc gtcgaaccgc ttccaatagc
      541 agtttccaga cttatgtttt tgccctctgc cagaaccctt aatccaccga cagttctcac
      601 tacaaggcgg gctaagtcta ccaccttttt ttcgcccatc ttgtcatgag catctatttc
      661 aaccagggat aagagatccg ttataaccgc gtttaaacga tcaatttcct tattgatgtc
      721 ccccaggaat tcaatgtata tttcgggaac ctcgccgccc atatgaatca atgattctgc
      781 caagacttta atcgcactta gcggggtctt gagttcatgg gaggcattgg aaacaaactc
      841 gctccgagcc ctgtctaggt tctccagctt ttcgctcata atattaaacg cttctcccaa
      901 ttcacgaaat tcccccacgc ttctgacctt gaccctttgt cccaggtggc cctggcccat
      961 ctttcgaatc acatccgtca atgattttat aggcctggtt atataggcgc ttataccgat
     1021 actgattaag ataacaaagg ctataattac cagggaggat ctatagagca ttgcacctat
     1081 tgcttcaagt atttcgccta tatctgaaag agaggtggat aaaataactg cgccggtcac
     1141 tttttggctt tcgtaaatga taggtgaata tgaatacatt acccattttt gatcggaaaa
     1201 tctatcccct atatgtaggg ctgtaggatc tacatcattg cctgccagca gatcatctat
     1261 tataggaaaa tcatttcgga ggttcctctt taagaatccg tgatcgctta aatcattata
     1321 ggaatcaaaa tctacaatac cattggcatc caggatcaga attctggtgg gttgaccccc
     1381 ctcacgatac attatatcct cccccagctg gctgatgtca aatattatat tttccttcat
     1441 ttcagtattt gagccggaat aatccttgga tataacttgg gctacctgta ccgcatacgc
     1501 cctgaagcgt tcttgcttat cgtggatttg tcgattctca aatactttta taatggataa
     1561 attcgtaacc acaaagccta tcccaatgag tatgagatag gcaatcgcaa ttctccagcg
     1621 taggctataa aaggctttct tatttatcca caaagtagta ccccactccc catttagtaa
     1681 agataaactc gggctggctg ggatttgtct caatcttctc cctcagcctc cgtatatgga
     1741 catctaccgt tcgcaaatcg cctagataat cgtatttcca tacggtctcc agcaagctct
     1801 ccctgctgta cactttaccc ggattggaag caaataattg gagaaggtcg aattctttag
     1861 cggttaaatt cacatcctcc ccgtctttaa taacgcttct gttggctata tttatggtta
     1921 tgttatggat atcgataact tgctcctggt ttctgccttc gctatgatta gatcttctaa
     1981 atatcgcctt aattctcgcc ttgagttcga gaatgttaaa tggctttgtt atataatcat
     2041 ctgccccata ttcaaggcca aggatcttat ccatatcctc accttttgct gtgagcataa
     2101 taacgggaac ggacgatttt tcccgtatcc gctgtaacac ctcaaaacca ttcaatccgg
     2161 gaagcatgac atccaacacg actaagtcaa attcctcgcc aaagaactta tctacagctt
     2221 cctgcccgtc ataggcctct acggtactgt aaccatcctg ttctaaacta aattttagtc
     2281 cttttataat tgcgggttca tcgtctacta ttaatagatt cttttgcatt acgtgtccct
     2341 tccctttcct tattaagtgc cttcgaaaag taatcggtcg gttgaaatgc cgtggctgct
     2401 taccaaagca tcacaattag atactatcat acgcatccat taaaatcaat ccgttatggg
     2461 ggcctattcc atggtatgaa tgtttctaaa ggttgcagca aattaggatc atttgatgcg
     2521 gatgaaatcc tttaaaccat caaaggtctt ctgtccgatt cctggtacgt tcatcaggtc
     2581 ctcttcggtt ttaaagatgc cgttctgctc cctgtaattg ataatattgc cggcaattac
     2641 cggtccgata cgcggcagcg tttccagctg tttttgatcc gccgtattta tatctatttt
     2701 agagtcacct tcgttaacta tcgcaccggc agaatttatc tccggaccct cttcgcctat
     2761 cttggggata atatacatac cctcatcctg cacccgcagg gccaaattga tcttcaatag
     2821 atccgcctca tccagaaagc ccccggccat ctccgcagcc tcgatgaggc gggattcggc
     2881 gggaacctca attacgcccg ggttacggac ctgaccggtt atgtaaattt ttatgatatt
     2941 gggcatctgg ggttcgcttt gatcttcctg ttgcccgcca ttgataccgt cccggctttc
     3001 tacctcggat gaagtttccc gaggcacaac ttctacgccc ccgggcttgc ccggatgaaa
     3061 aatactgtcc ttaaagatga tggatgagag tgttccgatc attataatcc caacaatcgc
     3121 tatgaacttg atgtgcttgt tttgaatttg aattatggtt atcatccccc atccaattca
     3181 ccgccatttg tgcgctttac gtcaaagatc ctacattaac attttatgct aaatggctgt
     3241 atcactaccc tataaatcgg tactcgatgc cgtacgacat tatccatata ttcattattc
     3301 tcccccatcc tataaattcc tcttttttca tatcgtttta tagccttttt tctgtacaaa
     3361 accatggccc ttctgttata c
//
LOCUS       DASRNX010000254        17604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_794751,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000254 DASRNX010000000
VERSION     DASRNX010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17604
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794751"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..900)
                     /locus_tag="VFD89_10080"
     CDS             complement(154..900)
                     /locus_tag="VFD89_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZJ58564.1"
                     /translation="MRDFSVVMINPKKDPTGFADVGHTELVVLAGDSIEDVKNRDVNN
                     YLNEWAKYAREHGVYIVPGLYIEDDYLCLCLIDDNGRVMGSQRACHLNTRSFPELRRG
                     DNINIIDTPFAKIFLCVDVDTLKPQIIRAAVLMGAEVVVSVQYIAPDRYNESMILAGA
                     WQNAQQNCIYILNAHNLGANIIGPCETSEDLSGFIKRMGADSLDIRAELSVSSRDKAY
                     GNFPIFKTLNRDLYNRYFGDHSHAARRKKS"
     gene            1134..2081
                     /locus_tag="VFD89_10085"
     CDS             1134..2081
                     /locus_tag="VFD89_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZJ58565.1"
                     /translation="MSFSISGCKQQKGQKVRLMEVTHSVFYAPQYVAIHKGFFEEEGI
                     DIELTTGEGADKVMTAVLAGQVEVGFMGPEAAIYVYNEGKEDHALIFAQLTQTDGSFL
                     MGREEEPDFKWEDLKGKTIIGGRKGGVPEMTLEYVLKKHGLTPGEDVEVLTNIQFALM
                     GGAFTGGTGDYVTLFEPIASTLENEGVGHVLASVGVAGGNIPFTAYAAKKGFIEKNKD
                     LMQRFTNAIYKGQQFVQEHSPEEIAEAIKPAFPDSDLQLLTMVAKRYKEQDSWPTGPA
                     LKEEDLNHLQDIMEMAGELDERAPYNKLVTNEFAEKAAK"
     gene            2248..3021
                     /locus_tag="VFD89_10090"
     CDS             2248..3021
                     /locus_tag="VFD89_10090"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58566.1"
                     /translation="MNNKVVVNLENVSLKYHSLEGETHAINDISFKVAEGEFIGIVGP
                     SGCGKTTILSLIAGLIKPTTGQVLVDGQRIEGPTTSVGYMLQQDYLFEWRNIIQNVLL
                     GLEIRGRVTEDSKIKAERLLETYGLGDFKKHYPDQLSGGMRQRAALIRTLAIDPKILL
                     LDEPFSALDYQTRLVMSDEVSTIIRKEGKTALLVTHDISEAISMAHKVIVLSERPATI
                     KNIHEIQLTSEDNNPISRRQAPEFREYFNTIWKELDVHV"
     gene            3014..3832
                     /locus_tag="VFD89_10095"
     CDS             3014..3832
                     /locus_tag="VFD89_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZJ58567.1"
                     /translation="MYNRNSNNAVSKEHAKYIAKIKRNKLMVRLSQAIILIAFIALWE
                     LAARLHFIDAFITSQPSRIISTIRNLHEEGVLLMHVSITIYETIVGFVLGTVLGTAAA
                     VVLWWSEFAAKVADPYLVVLNSLPKVALGPIIIVWIGAGPFAIITMALLVSLVVTIIT
                     VLNGFLEVSPDKIKLLQTFGANKMQILKKVILPASIPTIISAVKINVGMSWVGVIVGE
                     FLVSKAGLGHLIVYGGQVFKLDLVMTSVFILSVAAALMYQFAAWLEKKFLKWQQ"
     gene            complement(3943..5730)
                     /locus_tag="VFD89_10100"
     CDS             complement(3943..5730)
                     /locus_tag="VFD89_10100"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HZJ58568.1"
                     /translation="MKKTFIITLALILLIMDFGLLTCLAEGEDADPWTVESYTAFADI
                     GSDGSMDIEEYITYKFTKDFPGTLKRRMDTFAASGLDGLSIHSVDELDPEDIRNSKKE
                     TLQGDAYDVVYDTDYGLVNEIDITLDEEDRGKTLVFKYKLYDLVGLYKDMALLDWTFM
                     DDVDSREIKDIFVSVNLPKGANPDTVYSTLLGPLYSQQLGEDNNISYEASLMDGGRSL
                     RLTMLLPTSTIPGGRKMIDNEITKEVLSDMEEYESEIEATRKDYETRLLTIDLLTYIT
                     IGVIILVGVYLYFRYDRDPKSDTKLTHTNKLPADYYTPAELGVFMNKGKVRGNYMVAT
                     LMDLVNRGYLNIKPSAEDRYILSKNSDADARQLKAHEEYLLAWIFDDFGEDSPEVSIS
                     QFDDWLGDHSYRERYKYKYRTWSDLVEKQAAKWKFFENVRVAKLYGLLFGILTAVPGI
                     LLYTLDARINGIAIIALAMVLMIYCQSVRKRTEFGAINRTQWIAFRNYIREASASQLP
                     RPELDQWERFLAYVIPMGEAETMIGQLKQIYDNKALKDDRLSLLHGDNIENTLYWINS
                     LDTSNGLWAKLLGLCRFSGFKNLHTTKKG"
     gene            6246..8417
                     /locus_tag="VFD89_10105"
     CDS             6246..8417
                     /locus_tag="VFD89_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HZJ58569.1"
                     /translation="MKWVKRISIYMTILALGAAFVYFFVIKNPASRALNELLTHINKT
                     ENISYKDYIMPELHGDPFLEIIADRKIITGHRILGSQKNDQNGIKVNVSLTTGYGAFD
                     AGFVMTKINNRWLVSQFPRANQIGAAVPLWAEERGGGAMEYKVDVAGTPLSCILPSKG
                     DTINTGIPISLILIEDYIAEYQHLIPMHLHRIMSASGEFIEDAVLGYIPVESSLSVYQ
                     AVADVSTFLNKDVLPIGISDVTLYHSPSYKNKDLVAYIDPSNMPNDTIRIALNDNEFQ
                     GLSHQEVLISSSQGAFIKNLVDNLEYSIEPDEVLTFRHDSKPGSSVYRRGELLGSSHN
                     RWYIIPKNGGNLKINSINRTQSRASGGTVYRGNIEISGNQDGLTIINEVGLEEYLYSV
                     VPSEMPIKFGLESLKVQAIAARSYAVRCFKSRGYASLGAHVDDSVSSQVYNNIEETPI
                     AIQAVEETKGIIPVFGGDVIDARFFSTSCGYTSNFHETWSVDDIFPSAEIPYLVSKPQ
                     FPGDVPSLYNEENFKAFIEQKELKGYDLFSPFFRWSLTMKRQQIEAALHKNLPALQKA
                     QPLFVLTRDKDGSFTQNPIPEDIGQLQNIAPVSRGQGGNIMELEVTTTRGIYKIIKEL
                     NIRQLLKPVNLVPGEDPIEVLRHDGSAIKDFSILPSAFFYADIHRDSDGNISDVVFTG
                     GGYGHGVGMSQYGVYGLSLLGKTYTEIIEHFYPGTELHNLY"
     gene            complement(8423..9772)
                     /locus_tag="VFD89_10110"
     CDS             complement(8423..9772)
                     /locus_tag="VFD89_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZJ58570.1"
                     /translation="MNPYKVIIPARIKLALDKLNGAGHEAYIVGGCVRDSLLGREPTD
                     WDITTSAIPERIKEVFNGYKQVDTGLKHGTVTVILEGEPVEVTTYRIDGRYSDGRRPD
                     TVFFTEDIAGDLARRDFTINACAMTESELVDPFGGEQDIRDGRIRCVGDPRERFREDA
                     LRILRGIRFASVLGFKVEEKTKEAMLDCRQLIENVSAERITVEFCKILLGETVHDTLL
                     EFQTIIAFILPEIGDMIGFDQRNRHHIYDVYRHTLKSVEAIHRDIVLRVTMFFHDIAK
                     PACFSSDENCVGHFYGHARESALMTERILQRMRFSNREIRDITELVRYHDRPIGSTTK
                     SVKRLLSRIGEEQFRRLLKVKRADTMAKNPVYWESKLRDLEAIEGILDRLIAEGACIT
                     RSQLAVNGNDLIGLGIPEGKIIGYILDNLLKAVIRGDTANKRDDLLRRAEALWKQSL"
     gene            complement(9929..10129)
                     /locus_tag="VFD89_10115"
     CDS             complement(9929..10129)
                     /locus_tag="VFD89_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58571.1"
                     /translation="MKWTAILKDLKDDVGIVCPQNARGDIIIPIEYLPQGCRVGDVLQ
                     FKVSFDPFATLTLLNRSQPGDN"
     gene            complement(10154..11872)
                     /locus_tag="VFD89_10120"
     CDS             complement(10154..11872)
                     /locus_tag="VFD89_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZJ58572.1"
                     /translation="MARELFARTQKKVIPFDQNGQFYHRKARKHMDNNNYLNALNLYR
                     KAVEKEPQNVEYLLDLAEVFTEMGCFDQSSRVLFTILQKEKARFDCYFGLGCNFLGMQ
                     DYDKAMECFEKYLDIDPYGMYSDEARDLLEVLEDYEYYYDDNPDGFDSDEGNIYTLAT
                     EGKDLLDRGEYKKAIKKLEKVVRLDPNLLFVKNNLALAYFCAGRLNQAIDLGLDVIEK
                     DPQNIHANCNVCLFLNERDGKRGSEKYLDAIINFRTQDPEEIHKIAVTLCELNEHDRA
                     NQVLRRLLQYKPYDIRILHYVAASCFNLGKYREAYKYWRKIDRIDPDNNVSDFYLRLV
                     QEYLDGEKDGGEISYHFQVPYDEILLRIKELNDILKLDEKDLLIKWKHDDSFRTLLYW
                     GLELNDELIKKAILNVVASFNDGKSEEFLREFLLRITESEELKTEALGLLKQMAAKEP
                     YMAYVDEGLVEVKVDILEGDSFQIPVEMEMVIHIAGHMMEGRYGEGYEEDIKEIWTEF
                     VKSLYPDRLPRIRKHEAWAAAVELYYCMEQGIYVKNREVSDYYGISYSALYNNYNRLK
                     AVLRGK"
     gene            complement(11947..12831)
                     /gene="hslO"
                     /locus_tag="VFD89_10125"
     CDS             complement(11947..12831)
                     /gene="hslO"
                     /locus_tag="VFD89_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HZJ58573.1"
                     /translation="MEDYIIRATAAQGNIRAFAATTTNLVGAAQKTHDLYPVPSAILG
                     RTMTAALMMAVDMKSESHALSIILKGGGPIGNVAVVAKSDGRIKGYVDNPHVGLPLNS
                     LGKLDVGMAVGNQGKLTVIKDLGLKEPYAGQVDLMTGEIGEDIAYYMAVSEQKPSAVA
                     LGVLVNPDASVKAAGGFIIQPLPGAPEHLIDTIEERIAKVPAISTMVDEGKSPEEVLE
                     AVLGPMDLRVNHRIYPEFACDCNRERLESVLLSIGEEELETILKEDGKAELVCHYCNT
                     VYNFDEEDITKLLENARA"
     gene            complement(12891..13631)
                     /locus_tag="VFD89_10130"
     CDS             complement(12891..13631)
                     /locus_tag="VFD89_10130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZJ58574.1"
                     /translation="MYGDFAYIYDKMMYDVDYIKWADYIEDLFRHYGIRPVDIADLAC
                     GTGNITAILAERGYSVMGIDSSEDMLAVAHEKAYGKGLRIPFICQDLRRISLHQRVDA
                     ALIICDGINYILEDSDLDEVFSGIYSILKPHGVLLFDISSRYKLSSILGNNIMVDDDP
                     DISLIWQNDYDEEEGICTMDLTFFVRKGSLYRRFDETHIQKAHRACDIVGKLKQNNFV
                     DIKCYGHMGFTEPDKDSQRLMFASRKPK"
     gene            complement(13708..14556)
                     /locus_tag="VFD89_10135"
     CDS             complement(13708..14556)
                     /locus_tag="VFD89_10135"
                     /inference="COORDINATES: protein motif:HMM:NF016726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HZJ58575.1"
                     /translation="MFGGFFNRMYYGNPNKPDLKKEDVHGGRFKLFFVVLQVRVWKLI
                     QLNLLYSLFWAPAFFVTYTHLMAMSQMLENNDMVGNLPGFWTSLLILLIPCMLIAGPA
                     TAGTVYVIRNWARDEHAWLWSDFKDVWKQNWKGSLLVMLINGLALFVFYVNMNFYAVQ
                     AQKSLIFIVLRYLIVIVGIIYSMMNMFIFPMLVTYKLRLRQIFKNALIFTMAELPRAF
                     VIFVLAFLLFGVVFYYSMPFIVPFFIIGLTFPMFIVVSFANWVFDKYLNRELGEHGDA
                     LEQNGS"
     gene            complement(14621..15496)
                     /gene="dapA"
                     /locus_tag="VFD89_10140"
     CDS             complement(14621..15496)
                     /gene="dapA"
                     /locus_tag="VFD89_10140"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZJ58576.1"
                     /translation="MREWGRLLTAMVTPFDKDMNVDYEKAARLGQRIVDDGTTALVVV
                     GTTGESPTITDEEKYELFRTLKERVNVPIIAGIGTNCTRATIETGKLAVEAKADGVLV
                     VVPYYNKPNQQGLFAHFKTVAEAIDLPMMPYNVPSRTGGNMEAETSIALSKIPNIVAL
                     KEASGDIDQITRIIAGCHRDFLVYSGDDGFTLPTLCAGGYGVVSVAAQVAGQKIRDMI
                     EAYVDGNINKAAEIHRMLNPLFKALFVTTNPIPVKAALNLMGLDVGGLRLPLVEADDG
                     VKEILNRELSALGLI"
     gene            complement(15649..17445)
                     /locus_tag="VFD89_10145"
     CDS             complement(15649..17445)
                     /locus_tag="VFD89_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HZJ58577.1"
                     /translation="MVNKGNQTWTPITGDLMTDWVEEVSADEVLTEYPRPQMVREHWQ
                     SLNGLWDYAIGQVNGEVPRYEGKILVPFPIESSLSGVKSPLKPDEILWYRRNFKIPGS
                     WKDKRILLHFGAADWKTGVWLNGHTIGEHTGGYYPFTFDITEHLRDGDNELSVSVWDP
                     TNTFGQERGKQSLHPRGIFYTAVSGIWQTVWLEPVPDVYIKSHRLTPDLDKQLLNLRV
                     ETDGLIETTGSHGGLEYRATVYKNGNEIVSGGDNISNTLSLRIPEPRPWHPEDPFLYD
                     IRIELILDNIVKDEAKGYFGMRKYSVGRDELGVRRLMVNNEPLFQNGILDQGYWPDGL
                     YTAPTDEALKYDIEVAKLLGFNMIRKHAKAEPARWYYHCDRLGIIVWQDMISGGKNIN
                     IYLNGIIPMVAGGLTIKDDKYRRVGRQDPGNRQNYRKELKEMIDALYNFPSIGMWVPF
                     NEAWGQFDAVEIADWIEDYDGTRWIDHASGWHDQGTGDIKSVHIYFRRLKMPKNIKDR
                     AVVISEYGGYSYPIKGHIWRDGKEFGYKKFKSIDDFEDAYRDLSKSQVEPLRQTGLSA
                     AVYTQLTDVETEVNGLITYDRKRIKLNFMDRG"
BASE COUNT         4627 a         4483 c         3605 g         4889 t
ORIGIN      
        1 tcgatatagg ccctgatcct tttggggctg ttttttatag cggcaacctt aactaacggt
       61 ttcaatatcc aggtaagatt catccctgtc cctcccttaa actatggctt tttttaaaag
      121 gcgtacgggt tttacacggg ctacgggttt ggactatgat ttcttccgcc tcgcagcatg
      181 gctatgatct ccaaagtatc tattgtaaag gtccctgttt aaagtcttga atatgggaaa
      241 attcccatag gctttatccc ttgaggaaac ggacaactcc gcccgtatat cgaggctatc
      301 cgcccccatc ctctttataa aaccgctaag atcctcggat gtttcgcagg ggccgattat
      361 atttgcgcca agattatggg cgtttagaat gtatatacag ttttgctggg cgttctgcca
      421 ggcacctgcc aggatcatgc tctcattata gcgatccggt gcgatgtatt gaaccgacac
      481 taccacttcc gcccccatga gcacagcggc ccttattatc tggggcttta acgtatctac
      541 atccacacat aggaatatct tggcaaaggg ggtgtcgatg atatttatat tgtcgccccg
      601 ccgaagttcc ggaaagctgc gggtgtttaa atgacacgcc ctctggctcc ccataaccct
      661 gccgttatca tctattaggc atagacaaag ataatcgtcc tctatgtaga gcccgggaac
      721 tatatatacc ccatgttccc ttgcgtattt agcccattca ttcagataat tgttgacatc
      781 tctatttttt acgtcctcaa tggaatcccc ggctaatact acaagttccg tatgacccac
      841 atccgcaaaa ccggttggat cctttttagg atttatcatg acgacactaa aatctctcat
      901 aacaagccct ccttgaataa agcctatggc aaccgcttgt ttttattacc ccatggatat
      961 aacaggctta aggcttcttc cattatatca caaacaggcc aaaagctctg cttctttcac
     1021 ccttttttct tttctgaata tgatgaagta tcaaaatatg tttttgtttc ggaggtgtta
     1081 tttaagtgag atctataagg tatacccttc tgatattggc ccttttactt gcgatttcgt
     1141 tttcgatttc cgggtgtaaa cagcaaaaag gtcagaaggt tcgtttaatg gaggttaccc
     1201 attctgtttt ttatgccccc cagtatgttg ccatacataa ggggttcttt gaggaggaag
     1261 gcatagatat cgagctcact acaggcgaag gtgcagataa ggtaatgaca gccgtgctgg
     1321 cgggtcaggt ggaggtaggt tttatggggc ctgaagccgc tatctatgta tacaacgagg
     1381 ggaaggaaga ccatgcgctg atatttgccc aattgaccca gactgacgga tccttcctaa
     1441 tgggacgcga ggaagaaccg gactttaagt gggaggatct aaaggggaag accatcatcg
     1501 gcggccgcaa gggcggtgtg cccgagatga ccttagagta cgtgctgaaa aaacacggcc
     1561 tgacaccggg agaggatgtt gaggtcctga ccaatataca atttgctttg atgggaggcg
     1621 cctttacagg gggtacaggt gattatgtaa cattgtttga gcccatagcg tctaccctgg
     1681 aaaatgaggg tgtcggccac gtccttgcct ccgtcggggt tgcaggcggc aatataccct
     1741 tcacggctta tgccgccaag aaaggcttta tcgaaaagaa caaggacctt atgcagcggt
     1801 ttaccaacgc tatttacaag ggacaacaat ttgttcagga acactccccg gaggagatag
     1861 ccgaagccat taaacctgcc ttcccggaca gcgatttaca attgcttacc atggtggcaa
     1921 aaaggtacaa ggaacaggat tcatggccga cgggcccagc attgaaggaa gaggatctaa
     1981 accaccttca agatattatg gagatggccg gtgaattgga cgaaagagcc ccttataata
     2041 agttggttac caacgaattc gccgaaaagg cagcaaagta gtggctgcgc ccgatgacat
     2101 atcaggattc tagccggatg gccccttagg gggccatttg ctttaacaaa aaaacttacc
     2161 gggaatataa tgaactataa atgaaaatgg ggcaggagag agccccgtta caccgtttac
     2221 atcgctgtat ataggaggac tcggtttatg aataacaaag tagtggtaaa tctagaaaat
     2281 gtctccttga aatatcatag cctagaagga gaaacccatg ccataaatga catatccttt
     2341 aaggtcgcag agggcgaatt catcggcata gtaggcccca gcggatgcgg taaaaccacc
     2401 atactatccc taattgcagg gcttataaag cccacaacgg ggcaggtatt ggttgacgga
     2461 cagaggatcg agggcccaac aaccagtgtc ggttacatgc tacagcagga ttacctgttt
     2521 gagtggcgta atataataca gaacgtacta ttgggcctag aaatccgcgg aagggtcact
     2581 gaggattcta agataaaggc agagaggctc cttgagactt acgggctggg agactttaaa
     2641 aagcattatc cagatcagct ctcagggggc atgcgtcaaa gggcggcctt gatacgtaca
     2701 ctggccatcg atccgaaaat acttctgctg gacgaaccct tctctgctct ggattatcag
     2761 accaggctgg ttatgtccga tgaggtaagc accatcatcc gtaaagaggg caagaccgcc
     2821 ttactggtta cccatgatat ttcggaggcc atcagtatgg cccacaaggt catagtcctt
     2881 agcgagcggc ccgccaccat aaagaatatc catgaaattc agcttacatc cgaagacaac
     2941 aatcccataa gccgtcgcca ggccccggaa tttcgggagt attttaacac aatctggaag
     3001 gagttggacg tacatgtata acagaaattc aaacaatgca gtatcaaagg aacatgcaaa
     3061 gtatatcgct aagatcaaga gaaacaagct tatggtccgg ctcagtcagg caatcatctt
     3121 aatagccttt atcgccttat gggagttagc cgccagattg cattttattg atgcgttcat
     3181 caccagccaa ccttccagga taataagcac catcaggaac ctccatgaag agggtgtact
     3241 gcttatgcac gtctccataa ccatctatga aacaatagtg ggtttcgtat tgggtaccgt
     3301 acttggcact gcagccgccg ttgtattgtg gtggtccgaa ttcgcagcca aggtagccga
     3361 cccctatctg gtggtcttaa acagcctccc caaggtagcc ttagggccca taatcatagt
     3421 atggataggc gcagggccct ttgcaataat caccatggct ctactggttt ccctggtcgt
     3481 caccatcatt accgtactga acggcttttt agaggtcagc cctgataaga tcaagctgct
     3541 tcaaaccttc ggcgccaaca agatgcagat ccttaaaaag gttatcctgc cggccagtat
     3601 ccccaccata atttccgcgg tgaaaataaa tgtgggcatg tcctgggtag gggtaatagt
     3661 gggagaattt ctggtatcca aagccggatt gggacatttg atcgtatacg gcggccaggt
     3721 atttaagctg gatctggtaa tgaccagcgt gtttatactg tccgttgccg cggcccttat
     3781 gtaccagttc gccgcctggc tggagaaaaa attcctaaaa tggcaacaat aaaaaagccc
     3841 gaatcataaa ttttggcggt tcaacgtttt tagaggctta ttccatcgcc ggttacccat
     3901 tgggcaaagg ccgaagaaaa aagggatggg gcgaaatcgc cctcatccct ttttagtagt
     3961 atgcaaattc ttaaaccccg aaaacctaca tagccccagc agtttcgccc ataatccgtt
     4021 gctggtatcc agggagttta tccagtatag ggtattttct atattatcgc cgtgaagcag
     4081 cgacagcctg tcgtccttta acgccttatt atcgtagatt tgttttaatt gtccgatcat
     4141 ggtttccgcc tcacccatag ggatgacata ggctaaaaac ctttcccatt ggtccaattc
     4201 aggtcttgga agctgtgaag ccgaggcttc tcttatatag tttctaaagg caatccattg
     4261 ggtcctgtta atggcaccga attccgtacg ctttcggaca gattggcaat atatcataag
     4321 aaccatagcc agcgcaataa tagctatccc gtttatccgg gcatccagtg tatacagcaa
     4381 aatgccggga acagcggtaa ggattccaaa taacagccca tagagcttgg caaccctcac
     4441 gttctcaaag aacttccact ttgcagcctg cttttccacc agatccgacc aagtcctata
     4501 cttatatttg tatctctccc tataactatg atcccccagc caatcatcaa actgagaaat
     4561 ggacacttcc ggggaatcct cgccaaaatc gtcaaaaatc caggccagta aatattcctc
     4621 gtgagccttt aattgacggg catcggcatc ggaatttttc gacaggatat atctgtcctc
     4681 cgccgagggt ttgatgttca ggtatcccct atttaccaga tccataaggg tagctaccat
     4741 gtaattgccc ctaaccttac ccttattcat aaagactccc agttccgccg gagtatagta
     4801 atccgccggc aacttattgg tgtgagtcag cttggtatcg gatttaggat ccctgtcata
     4861 tctaaaatag agatataccc caactaatat tattactcca atggttatat aggtgaggag
     4921 gtctatggta aggagccttg tctcgtagtc ctttctggtc gcctcaattt cactttcgta
     4981 ttcctccatg tcggataaga cctctttggt tatctcattg tctatcatct ttcggccgcc
     5041 gggaatagtc gaggtaggca gcagcatagt aagcctcaag cttcggcccc catccattag
     5101 gcttgcctcg taggatatgt tgttgtcttc cccaagctgc tggctataca acggtcctaa
     5161 caacgtggag taaacggtat ccgggttcgc gcccttcgga aggttaacgc ttacgaatat
     5221 atccttaatt tcgcggctgt ccacatcgtc cataaacgtc cagtccaaca atgccatatc
     5281 cttatacagc ccgactaagt cgtatagctt gtatttaaag acaagggttt ttcccctgtc
     5341 ctcttcatca agggttatat ctatttcgtt gaccagccca tagtccgtat catagaccac
     5401 atcgtaggca tccccttgaa gtgtttcctt tttagagttt cttatatcct ccgggtcaag
     5461 ctcgtcaacg ctatgtatgc ttagcccgtc caaccccgac gcagcgaagg tatccattct
     5521 cctcttcaag gttccgggga aatccttggt aaacttatag gtaatatatt cctctatatc
     5581 catagagcca tcagacccga tatcagcaaa ggccgtatag gattctactg tccaaggatc
     5641 agcatcctcc ccttccgcca ggcaagtcaa cagaccaaag tccattatca acaggatcaa
     5701 tgccagtgtt attataaatg tttttttcaa cttccccacc ctttcatata atcagccaag
     5761 ccgcaggtct tcctcggata gcttaatgcc cggacgattt tatggatagt ttctttcgtt
     5821 tattttgaat tttagtatat cagtctgtac atattatgta ttctaccatg aaacccccca
     5881 tttctccata ctttttttat tacaaaaaaa cagccgggtt ccgcagctaa aaacagggcc
     5941 gtgtaacctt tacgggaatt tattccactg ccttatctgt agataaacaa cccaaactat
     6001 aaaacttgtc acttatagat aaatttattg ggcctattgt ttacatatag ataattgcca
     6061 aataatcgca tctttaatca tggaagatcc aaaatatgat tcccgactat tgtactttat
     6121 agtgcgaaac catgatataa taattatata tgacgcaatg gtcacagcga ttgcttccga
     6181 tgttataagg ctccggctcg ctgtgtatgc atcgcttatc tataatccta accgggagtg
     6241 gtacaatgaa atgggtcaaa cgaatatcca tatatatgac catccttgcc ctgggggccg
     6301 catttgtgta cttctttgtg ataaaaaacc cggcttccag agcccttaat gaactgttaa
     6361 cccatataaa caaaaccgaa aatatttctt ataaggacta catcatgccc gaactccacg
     6421 gggatccctt cttagagatc atagccgatc gtaaaattat aacaggacat cgaatcctgg
     6481 gctcacagaa aaatgatcaa aacggcatca aagtaaacgt atccctgaca acaggatacg
     6541 gggccttcga cgccgggttt gtgatgacaa agataaataa tagatggctg gtatcccaat
     6601 tcccccgggc gaaccaaata ggggcggcgg tacccctatg ggccgaagaa aggggcggcg
     6661 gggccatgga atacaaggta gacgtagccg gaaccccgct tagctgcata ctcccatcca
     6721 aaggggatac aattaatacc ggcatcccca tctccctaat cctcatcgag gactatattg
     6781 cagaatacca gcacctgatt cccatgcacc ttcacaggat aatgtccgct tctggagaat
     6841 ttatagagga cgcggtcctt gggtatatac ccgtcgaatc ttccctttcc gtataccagg
     6901 cagtagcgga tgtctccact tttttaaata aggatgtcct gcccataggt atctccgatg
     6961 ttaccctcta tcattctcct tcctataaga acaaggatct cgtagcctat atagacccct
     7021 ccaatatgcc caacgacacc ataaggatag cgctcaatga caacgaattt caaggcctgt
     7081 cccatcagga ggtcctcata tcctccagcc aaggcgcctt tattaaaaat ctggtggata
     7141 acctggaata cagtatagag cccgatgagg tgttgacctt taggcacgat tcaaaaccgg
     7201 gttcatcggt ttatcggcgg ggcgagctct taggatcgtc ccataatcga tggtatatta
     7261 tccctaaaaa cgggggaaat ctgaagataa acagcataaa caggacccaa tcccgggctt
     7321 ccggggggac cgtctatcgg ggaaacatag aaatttccgg caatcaagat gggttaacaa
     7381 ttataaacga agtgggtttg gaggaatatc tctattccgt ggtcccaagc gagatgccca
     7441 taaagttcgg actggaaagt cttaaggtcc aagctatcgc agccaggtcc tatgccgttc
     7501 ggtgttttaa gtctagggga tatgcgagcc tgggcgcgca cgtggacgac agcgtctcca
     7561 gccaggtata caataacatc gaagaaaccc ctatcgccat acaagcggtt gaagaaacca
     7621 agggcattat cccggtcttt ggcggcgatg taatcgatgc caggtttttt tcaacctcct
     7681 gtggatatac atccaacttc catgaaacct ggagcgtaga cgacatcttt cccagtgctg
     7741 agatcccgta tctggtatca aagccgcagt ttccaggcga tgtccccagc ctttacaacg
     7801 aagaaaactt caaggccttt atagagcaaa aggagctgaa gggctatgac ctgttctcac
     7861 cctttttccg ttggtcattg accatgaaaa ggcagcagat agaggccgcg ttgcacaaaa
     7921 acctgcccgc actgcaaaaa gcgcaacccc tatttgtcct gacccgggat aaggacggct
     7981 cctttaccca gaatcccata cccgaggata tagggcagct acagaatata gcacctgtca
     8041 gccgaggcca ggggggaaat atcatggagc tggaggtaac aactacccgg gggatataca
     8101 aaatcatcaa ggaactaaat ataaggcagc tcttaaagcc agtgaacctt gtaccgggcg
     8161 aagaccccat agaggttctg cgccatgatg gcagcgctat caaggacttt tccatactgc
     8221 caagtgcctt cttctacgct gatattcacc gtgacagcga cggaaatatc tccgatgtgg
     8281 tatttaccgg cggcggatac ggccatgggg tggggatgag tcagtacgga gtctacggcc
     8341 tttcattgct ggggaaaacc tataccgaga tcattgagca cttttacccg ggtaccgaac
     8401 tccacaacct atattaaaga ggttataatg attgtttcca tagggcctca gcccgtctta
     8461 aaagatcgtc ccttttatta gctgtgtccc cgcggataac cgccttcaaa aggttgtcca
     8521 ggatataacc tattattttc ccttcgggga ttccgagtcc tatcagatcg ttcccgttga
     8581 ctgccagttg acttcgggta atgcaggctc cctcggcaat aagcctatct aaaatgccct
     8641 ctatcgcctc gagatccctt agcttgcttt cccaatacac cggatttttg gccatggtat
     8701 cggccctctt gacctttagg agcctcctaa attgctcctc ccctatccgg ctcaggagcc
     8761 tcttaacgct tttcgtcgtc gagcctatag gtctatcatg ataccttaca agctccgtga
     8821 tatcccttat ctccctgttt gaaaatctca tcctttgaag tattctctca gtcattaatg
     8881 cagattccct tgcatgtcca taaaaatggc ccacacagtt ttcatccgat gaaaaacagg
     8941 cgggcttcgc gatatcatga aagaacatgg tcaccctaag gactatatcc ctatggatgg
     9001 cttccacact ctttagagta tgtctataca cgtcatagat gtggtggcgg ttgcgctggt
     9061 caaatcctat catgtccccg atttcgggca aaataaaagc tataatggtc tgaaattcca
     9121 ggagggtatc atggaccgtt tcccctaaaa gtattttaca aaattctaca gtgatccgtt
     9181 ccgccgatac gttttctatc agctgcctgc agtccagcat agcctctttt gttttttctt
     9241 ctactttaaa tcccaatacc gatgcgaagc ggatccccct aagaatccta agggcatctt
     9301 ctctgaagcg ctccctcggg tcccccacac agcgaatccg accgtctctg atgtcctgtt
     9361 cccctccaaa gggatcaacc agctcagatt ctgtcatagc acaggcattg atagtaaaat
     9421 ccctgcgagc caggtccccg gctatatcct ctgtaaaaaa taccgtatcg ggtctgcgcc
     9481 cgtcggaata tctgccgtct atcctataag ttgtgacctc aacgggttca ccctctaaga
     9541 tgacggtgac ggtcccatgc ttaagtccgg tgtctacttg cttatagcca ttaaaaactt
     9601 ctttgatcct ttcggggata gcggaggttg ttatatccca atcggtaggt tctctcccca
     9661 acagggaatc tcggacacag ccccccacga tgtaagcctc atgaccagcc ccattaagct
     9721 tgtccagagc caattttatc ctggcgggaa taattacttt atatggattc atttaatcat
     9781 acctcggtat atacaatcgc ccttcccggc ctaaatccca cgccctattc aacgtcaatc
     9841 cccatcaaat atcgggtttt cttccaatag tcataaaccc ggcgaaggaa acaaccatgg
     9901 cggaaatgct ggttgatcgg gaccgacctt agttatctcc cggttgtgat ctgttcaata
     9961 atgtcagggt tgcaaaggga tcaaaagaaa ccttaaactg aaggacatcc cctaccctgc
    10021 agccctgagg taaatactct atgggaataa ttatatcccc ccgggcattc tgcgggcata
    10081 caatccccac atcgtccttg agatccttta gtatagctgt ccatttcata ccccaaaccc
    10141 ctcctaaaac accctatttg ccccgcaata ccgctttgag cctgttataa ttattataga
    10201 gagcagaata gctgatccca taataatccg agacttccct gttcttaaca tatatcccct
    10261 gttccataca atagtaaagc tccacagccg cagcccaggc ttcatgtttt cttatcctgg
    10321 gcaacctatc gggataaagg gatttcacaa actctgtcca aatctccttg atatcctcct
    10381 cgtacccttc cccgtatcgg ccctccatca tatggcctgc aatgtggatc accatctcca
    10441 tctcaacagg aatttggaag gaatcgccct ctaagatatc caccttgacc tctaccaagc
    10501 cttcatctac gtaggccata tagggctcct tggcagccat ttgctttaaa agtcccagag
    10561 cttcggtttt taattcctcg ctctcggtga tcctaagcag aaattcccgt aaaaactcct
    10621 cggattttcc gtcgttgaag gaagccacta cattcagtat cgcctttttt ataagctcat
    10681 cgttgagttc taggccccaa tacaacaggg ttctgaagct gtcgtcatgc ttccatttaa
    10741 ttaacagatc cttctcgtcc agcttcagga tatcgtttag ttccttaatc ctcaggagaa
    10801 tctcgtcgta gggcacctgg aaatgatagg atatctcgcc cccgtccttt tccccgtcca
    10861 aatattcctg taccagcctc aaataaaaat cgcttacgtt gttgtccggg tcgattcgat
    10921 cgatttttct ccaatactta taggcttccc tgtatttgcc cagattgaaa cacgatgcgg
    10981 ccacataatg caggattctg atatcatagg gcttatattg cagtagcctt ctcagcactt
    11041 gattcgccct gtcatgctca ttaagctcac atagggttac ggcaatttta tgaatttctt
    11101 cgggatcctg ggtcctgaaa ttgatgatcg catccaggta tttttcactg ccccttttcc
    11161 cgtccctttc gtttaagaag aggcatacat tacaattggc gtggatattt tgggggtcct
    11221 tctctatgac gtccaaaccc aaatctattg cctggtttag cctgccggcg caaaagtatg
    11281 ccagggccaa gttgttcttt acaaacaata ggttagggtc cagcctaact accttctcca
    11341 attttttaat cgcttttttg tattctccac ggtctaaaag gtccttgcct tccgtagcca
    11401 aggtgtaaat attgccttca tcggaatcaa acccgtcggg attatcgtca tagtagtatt
    11461 catagtcctc caaaacttcc aataaatccc ttgcctcatc ggagtacatt ccataaggat
    11521 caatgtctaa atatttttca aagcattcca tggctttatc gtagtcctgc atgcccaaaa
    11581 aattacagcc caagccaaag tagcaatcga accttgcctt ttccttttga aggatagtaa
    11641 acaaaaccct gctggattgg tcaaagcatc ccatctccgt gaagacttcg gccaggtcca
    11701 ataggtactc cacattttgc ggttccttct ctaccgcttt tctgtacaag tttaaggcat
    11761 tcagatagtt gttattatcc atatgcttcc tggcttttct atgataaaat tggccgtttt
    11821 gatcaaaagg gataaccttt ttctgtgttc ttgcaaatag ttctctagcc atcttcatct
    11881 cctgataatt gaattccaca aacacattat agcataaaac ccatggggac tctatacaaa
    11941 gttgggctat gctctggcat tttccaataa tttcgttata tcctcttcgt caaaattgta
    12001 gacggtgtta caataatgac ataccaactc cgccttgccg tcctctttca aaatcgtttc
    12061 cagctcttcc tcccctatgc tgagaagcac cgattccaac cgttctctgt tacaatcaca
    12121 ggcaaattcg gggtatatcc ggtgatttac ccttaagtcc atgggcccca atactgcttc
    12181 cagcacctcc tcgggacttt taccttcgtc taccatggtc gaaattgccg ggaccttggc
    12241 tatcctctct tcaatggtgt ctatgagatg ttccggtgcg ccgggaagcg gctggataat
    12301 aaatccgcca gcggccttta cgctggcatc cgggtttacc aagacgccca gtgccaccgc
    12361 cgagggcttc tgttctgata cagccatata ataggcgata tcctcgccaa tctctcccgt
    12421 cattaaatct acctgcccag cgtagggctc ctttaaaccg agatccttaa tcacggtcag
    12481 cttcccttgg ttgcccacag ccatgcccac gtccaacttt cccagggaat tcagcgggag
    12541 tcccacatgg gggttatcaa catagccttt aatcctaccg tcggacttgg caaccactgc
    12601 cacgttccct atggggccgc cgccctttag aattatggat aatgcatggc tctcactctt
    12661 catatccacg gccatcataa gggccgctgt catggttctg cctaagatgg ccgagggaac
    12721 aggatataaa tcgtgggtct tctgggccgc tcccaccagg tttgtagtcg tagcagcaaa
    12781 agcgcgtata ttcccttggg ctgctgtagc cctgattata tagtcttcca tattctgtct
    12841 cctgtttttt tagattctag tttgtttttg acgtaacagt ccccaaacct ctattttggt
    12901 tttctggaag caaacatcag gcgttggcta tccttatcgg gttcggtaaa gcccatatgc
    12961 ccatagcatt ttatatctac aaaattattt tgcttcaatt tccccacgat atcacaggcc
    13021 cgatgggctt tctggatgtg ggtttcgtca aatctacgat agagcgaacc cttccgcaca
    13081 aagaaggtca agtccatggt gcatataccc tcttcctcat cgtagtcatt ttgccatata
    13141 agggagatat ccgggtcatc atcaaccatg atgttgtttc ctagtataga ggatagctta
    13201 tagcgggagc ttatgtcaaa caataatacc ccatggggct tcaaaatgct atatataccg
    13261 gaaaaaactt catcgagatc cgaatcctcc aaaatataat ttattccatc gcatattata
    13321 agcgccgcat caaccctttg gtgcagggaa attcgcctca gatcctgaca tataaagggt
    13381 atcctaagcc ccttcccata ggctttctca tgggctactg cgagcatgtc ctctgaactg
    13441 tcaatcccca ttacgctata accccgctca gccagtattg cggttatatt acctgttccg
    13501 caggctaagt ccgctatatc cacgggcctt atcccgtaat gacggaataa atcttctata
    13561 tagtcagccc attttatata atctacgtcg tacatcattt tatcgtatat atatgcaaag
    13621 tctccgtaca tcgtcaaaac atcctatatt atattttgtt tccgttttat gatcctatcg
    13681 ccggggacca aacaaaaaaa gggccattta agacccattt tgttccaagg catcgccatg
    13741 ttcaccgagt tccctgttaa ggtatttatc aaatacccaa ttggcaaagg aaaccacaat
    13801 aaacatagga aatgttaagc ctataataaa gaacggtact ataaagggca tgctgtaata
    13861 aaatactaca ccgaacaata aaaaggctaa tacaaatatt acaaaggccc tgggcaattc
    13921 tgccatggtg aatataaggg catttttaaa tatctgcctg agtctcagct tgtaggtgac
    13981 cagcatcgga aagataaaca tattcatcat ggaatatata attccaacta ttacgatgag
    14041 atagcggagc acaataaata tcaagctttt ttgtgcctgc accgcataaa agttcatatt
    14101 tacatagaat acaaacaatg ccagcccgtt gattaacata acaagcaggc tccctttcca
    14161 attctgtttc catacatcct taaaatcgct ccataaccac gcatgttcgt ccctggccca
    14221 atttctgatt acatacaccg ttcctgccgt tgccggcccg gctataagca tgcatgggat
    14281 taacagtatc aacaacgagg tccaaaatcc cggcaggttg cctaccatat cgttgttttc
    14341 taacatttga gacatagcca ttagatgggt atatgttaca aaaaaggccg gggcccaaaa
    14401 caaggaatat aatagattaa gctggatcag cttccaaacc ctaacctgca atactacaaa
    14461 aaatagttta aaccgtcccc catgaacatc ttcctttttt aaatcgggtt tattgggatt
    14521 gccataatac atccgattaa aaaatccacc aaacatctaa acagtccttt cccgggcaac
    14581 gaatgtcgcc ttctcttgta tttctaataa accaccatgg ctaaattaga cctaaagcac
    14641 tcaattccct gtttaagatc tctttaacgc cgtcatcagc ttccactaga ggcagacgca
    14701 gtcctccaac atccaatccc ataagattta atgctgcctt tacaggtatg ggattggtgg
    14761 ttacaaatag ggccttaaac aatgggttga gcattcggtg aatttcggca gccttattta
    14821 tattgccatc aacatatgcc tcaatcatat ccctgatctt ttgtcctgcg acctgggcgg
    14881 ctacactgac gaccccatat ccgcctgcac agagtgtggg cagggtaaat ccgtcgtccc
    14941 ctgaatagac taaaaagtcc ctgtggcatc ctgcgataat cctggtgatc tggtctatat
    15001 ctccgctggc ctcttttagc gcaactatat tgggtatctt ggatagggca atagaggttt
    15061 ctgcctccat attcccaccc gtccttgaag gaacgttata gggcatcata ggcagatcga
    15121 tagcctcggc tactgtctta aaatgggcaa aaagcccttg ttgattgggt ttgttataat
    15181 aagggactac caccaacacc ccgtcggcct tggcttctac agccaattta cccgtctcta
    15241 tggtagcccg ggtgcaattg gtgccgatcc cggctattat aggtacattt actctttcct
    15301 ttaaagttct aaacagttca tacttttcct cgtcggttat tgtcggggat tccccggttg
    15361 ttccaactac aaccagcgca gtagttccgt catcgacgat cctttgcccc aatcgggcgg
    15421 ccttttcata atcaacgttc atatccttat caaagggtgt aaccatagcg gtaagcaatc
    15481 ttccccattc tctcatacca atgcctcctc atatctgtgt aagaaaactt aattctattt
    15541 tactatatgt ctccggacat gtaaatgata tgaaataatt tagggtcctc cacaccctcc
    15601 ccatgtatgt aattcgggca taagtcgaat tgacattctc cccggcgact agcccctatc
    15661 catgaaattt aattttatcc ttttgcgatc ataggtgatc aagccgttaa cttcagtttc
    15721 cacgtccgtc aattgggtat agacggcggc agaaaggccc gtctgtcgca ggggctctac
    15781 ttgggatttc gaaaggtccc tgtaggcatc ttcaaaatca tctatgctct tgaacttttt
    15841 atagccgaat tccttaccgt ccctccatat atgtcccttt atgggatagc tgtatccgcc
    15901 atattctgat attaccaccg cacgatcctt aatgttcttt ggcatcttca gccttctaaa
    15961 atatatgtgt acgctcttta tatccccagt cccctgatca tgccatccgc tggcatgatc
    16021 tatccaacgg gtgccgtcat aatcttcaat ccagtcggct atttccactg cgtcaaattg
    16081 cccccaagcc tcattgaagg gtacccacat ccctatgctg ggaaaattgt aaagggcatc
    16141 tatcatctct tttaattctt ttctgtaatt ctggcgattt cccggatcct ggcgtccaac
    16201 ccttctatat ttatcatcct tgatggtcag accgcccgct accatgggta ttatgccgtt
    16261 taaatatata tttatgtttt tcccgccgct tatcatgtcc tgccacacga taatacccag
    16321 tctatcgcag tggtagtacc aacgggccgg ctccgccttt gcgtgcttgc ggatcatatt
    16381 aaagcccaag agcttggcaa cttcaatatc atactttaag gcctcatcgg taggtgccgt
    16441 atataatccg tcgggccaat agccttgatc caagatacca ttctggaaca ggggctcgtt
    16501 atttaccatg agcctcctga ctcccagctc gtcccttcca acgctatatt ttctcatgcc
    16561 aaaatagcct ttagcttcat ccttaacaat attgtccagt atcaactcta tccttatatc
    16621 gtataggaag gggtcttcgg gatgccaagg ccttggctcg ggaatcctca aagacagtgt
    16681 attggatata ttatccccgc ccgaaactat ttcatttcca tttttatata ctgttgccct
    16741 gtattccaac cccccatggg atccggtcgt ctcgatcaga ccatctgttt caaccctcag
    16801 gttcagtagc tgcttatcca aatccggagt cagcctatgg gatttaatat atacatccgg
    16861 taccggctct aaccaaacag tttgccatat acccgacacg gctgtataga atatacccct
    16921 aggatgcagg gattgttttc ccctttcctg tccaaaggta ttggtagggt cccaaacgga
    16981 tacggatagt tcgttgtccc catcccgtag atgttcggtt atatcaaagg taaatgggta
    17041 ataacccccg gtatgctccc ctatggtatg accattcagc cataccccgg ttttccaatc
    17101 cgcggcgcca aaatgaagca gaatgcgttt atccttccat gatccgggta ttttaaaatt
    17161 tcttctatac cacaaaatct catccggttt caagggactt ttaacgcctg aaagggatga
    17221 ttctattgga aagggaacaa ggatcttgcc ttcataccgg ggtacttcac catttacctg
    17281 cccaatagca taatcccata agccgttcaa actctgccaa tgctccctga ccatctgggg
    17341 ccggggatat tcggttagca cctcgtccgc actcacctct tccacccagt cggtcataag
    17401 atctcccgtt atcggtgtcc atgtttggtt ccctttattg accatatgta atcccccccc
    17461 caaatattta acccataaac ctcttaatcc attttagctt atccctatat ggtggatacc
    17521 taagggatat atcaatcagg ttagattttt ttactacgct cttatagtgg ctaaaagtat
    17581 caaagctagc cttaccatga tacc
//
LOCUS       DASRNX010000255        16019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_210987,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000255 DASRNX010000000
VERSION     DASRNX010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 16019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16019
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210987"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFD89_10150"
     CDS             <1..639
                     /locus_tag="VFD89_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZJ58578.1"
                     /translation="ILGYMPQQQDFYNDFTANRFLWYMCALKGIPRQRAGEMVDYVSN
                     LVGLGDALHKKLGAYSGGMKQRILIAQALLNDPKILILDEPTAGLDPKERIRIRNFIS
                     EIAINKIVILATHVVSDVEFIAREVMLLGEGRLIIKDIPARIIDNMVGRVFEANIREE
                     EIKEYQKIYKLSSISGDREGVRIRYITDGAKPGLGAIPVRPSLEEVYLDYFG"
     gene            793..2322
                     /locus_tag="VFD89_10155"
     CDS             793..2322
                     /locus_tag="VFD89_10155"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HZJ58579.1"
                     /translation="MDSKKKGRKVEDGRNFEPKENLTAVNGRPIAHNENIKTAGVRGG
                     VMLEDTWYLEKMSHFNREVVPERRMHAKGSGAFGTFTVTNDITKYTKADLFSEIGKQT
                     EMFARFSTVAGERGAADAERDIRGFALKFYTDEGNWDLAGNNTPVFFFRDPMLFPDLN
                     HAVKRDPRTNMRSAQNNWDFWSMIPEALHQVTITMGDRGIPKSYRHMHGFGSHTFSLI
                     NKDNERVWTKFHFVTQQGNEYLTDAEAEEVVARDRESNQRDLYEAIESGEYPKWTMYI
                     QVMTEEQAQNMPYNPFDLTKVWYHKDFPLIEVGYFELNRNPENYFQDVEQAAFNPADV
                     VPGIGYSPDRMLQGRLFAYGDAQRYRLGVNFHQIPVNRPKFEANYYHRDGSMRVDGNF
                     GSTLHYNPNSYGQWKDQNELTEPSYDGGGPADNYDFRNDDDNYYEQPGKLFRLMSDDE
                     KQRLFENTARNMTGTTELVQKRHIRHCYLADSAYGEGVAKALGIDINEVDMDDTYGAR
                     A"
     gene            2577..2912
                     /locus_tag="VFD89_10160"
     CDS             2577..2912
                     /locus_tag="VFD89_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HZJ58580.1"
                     /translation="MEAIHEALFLIIDLSVLFFEMIGVILIIVTGLRGVYILFIKKKT
                     KHHWLKSLLTALSFLLLAEVLETVKIRTINQLFTVGGLFGLRAIMVLYIDWERKHDEH
                     KEIQIQQYG"
     gene            complement(3128..3916)
                     /locus_tag="VFD89_10165"
     CDS             complement(3128..3916)
                     /locus_tag="VFD89_10165"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HZJ58581.1"
                     /translation="MHTKRSLKKDIEQAGIRKDGTLLIHSSMKAIGPVEGGADTVLDS
                     FIEHMKYGLLLFPTHSWGEDNLPDDVYDPIKEPACVGLLPNLFLKRTGVIRSMHPTHS
                     VAAIGARAQSYVLHDSDVHTPCPRHGCFGRLYDENAQLLFLGASLKTNTFIHSLEEWL
                     NIPNRINPQSRQIKLLLEDGSIRKIDFYGHSFVNGDVSQNYDKLLTPMLRMGIAKEAR
                     IGDALSYVVEVKPMADWVMGLLRENPGLFENDIPLRTPPTVKKQ"
     gene            4398..6296
                     /locus_tag="VFD89_10170"
     CDS             4398..6296
                     /locus_tag="VFD89_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZJ58582.1"
                     /translation="MYKDHKIIALCITQLHDERNFDFVSALNKKINDFGYKLFIYHSH
                     LDFYDERQNEKGDEAVFDLIDFDIVDAVIIYEESIRNQRISDGIVKKAKSKSVPVITI
                     GVTKQSTANFILNLEIGFEKVVRHVIEFHKIKDTIMIAGRRDSYYSNKRIEVYKKVLS
                     ENNIRFDDSMLYYGDFWSEPTCAVMQELIDNNRLPKAIICANDGMAIAAIDTLHENDI
                     FVPDDIVVTGFDGIRDARYNIPPVTTCSMNYEKLAYDIVLCIERLLSGKKINKENYTD
                     QTIEVYSSCGCNKVPDMEHGGLLRIVRDRYKRFLHYNDELHEMTERVFDSKTPEEIPR
                     ILEQYELYDCYVFVNDFFFDKSIRLTEDIKGKAFSDKLYLLYKTGLDADNYPMEFQRK
                     DILPDIDSLIQENDPIIFTSLSSRGLPFGFLCTAFKIDIENYSFLPQFTVFLSNALSG
                     YRNIRYLEDLSKRYEKLAEEDFLTGLLNRTGFYNEISRLKETIDTTVTENFIVTLVDL
                     DGLKQINDNHGHHAGDQAIITIANIIKTIPIENKICVRLGGDEFLICSLCTDKESTKK
                     IIRNHLFDNLKAINKYSANPFKISISIGFSSSGSGKIDIDNLLKQSDELMYNHKFEKR
                     QGRNTRYH"
     gene            6353..7546
                     /locus_tag="VFD89_10175"
     CDS             6353..7546
                     /locus_tag="VFD89_10175"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZJ58583.1"
                     /translation="MNKEKFLSCIQNEPIINSHSHHLQDVDHRGLNLQNVLRSSYVNW
                     CHKPVPDGNSKDETALWLNSVRNRSYFIWIEKAMMDLYGIDEHLDADTWYIFDGAIRK
                     AHSGGAWHLSILRDQCNYRAIFLDTFWKPGDDNNHPDLFKPAYRVNSLFYGYNRTARD
                     HNENNFQLLEDRSITDIDEYIKLIGDVIHRKREAGCTVIKCALAYDRSLGFGDGTKEN
                     AQRAMNENPDEDDIRAFQNYVFDRICEIAAELSMPVQIHTGLGLMAGSNAMQLQPLIS
                     RNPNTIFWLMHGSYPWTSDIAGLAHVYPNVWADLCWLPLISPTAAHRLMHELIDVCNA
                     DRVVWGCDTWTGEESYGARLAFLEILSQVLCERVDMGRMREGDAQRYARAVMYDNAAG
                     ILGDI"
     gene            7676..9112
                     /gene="pyk"
                     /locus_tag="VFD89_10180"
     CDS             7676..9112
                     /gene="pyk"
                     /locus_tag="VFD89_10180"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZJ58584.1"
                     /translation="MRKTKIICTLGPASDDPGILKALLEKGANAIRLNFSHGDHEEHG
                     KRIALVKQVREEMGLPIAILMDTKGPEIRIGNFKDGEVRLEEGQKFVLTTEEIQGDEN
                     IVTVRFKEITKDVCVGTAILIDDGLVALVVEEITQDAVHCKVINGGILGSRKGVNIPG
                     VSTSLPAITTKDAEDIKFGISQQIDYIAASFVRRASDVLAIKRVLSENNGDYIKVIAK
                     IENQEGLENIDEIIRVSDGIMVARGDLGVEIPTEDVPIHQKEIIKKCIEASKPVIIAT
                     QMLDSMIRNPRPTRAETSDVANAIYDGADVIMLSGETAAGKYPVETLLTMARIAKRVE
                     NSIDYEMEFEKRVAGKEATVTDSISHATCSIAHGLHAKAIVTATRSGRTARMVSKYRP
                     AAQIIATTTSDATCRELSLVWGVQPFVSPDMSDTDRMFEKSTEVAANTGIACSGDIIV
                     ITAGIPMGISGSTNLIKVSQIGDVPAGN"
     gene            9678..10022
                     /locus_tag="VFD89_10185"
     CDS             9678..10022
                     /locus_tag="VFD89_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HZJ58585.1"
                     /translation="MGDRFTLYNLKVEVVSSDKPMVCRHKEGDYFLVEGEDLIFPENN
                     SFSMYALSAILPLLPAKQRPLHPNDWMLSDSMIACPDPNCGAKFKITRIGTKEFSHAE
                     CTVEKIYDGEEE"
     gene            10022..11068
                     /locus_tag="VFD89_10190"
     CDS             10022..11068
                     /locus_tag="VFD89_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZJ58586.1"
                     /translation="MVQRVELSDGYTISRVINGCWQLSQGHNLIGSLDMKDIMRAFHM
                     LAEGGFTTFDCADIYTGAEEFLGEFIKELRRGSGISSHDIQIHTKYVPDINLLAQVDY
                     QYTEGIIDRSLKRLNRDVLDLVQFHWWDYDAPGCLETAGYLLRLKEKGKIRNIGVTNF
                     DTEHFANIVEAGIPIVSFQTQYSVFDRRPEKALTEYCRLKNISLLCYGTIAGGFLADR
                     WIGKTMDVPETRSQVKYLQVIEDTLGWDGYQDLLALLGRIGDKYGVRIAHVAVKYILS
                     QSGVGAAIIGVRNSRHVQSNTGIFEFDLDHEEIDEIRDFLDRYPILDGEPFELERTVG
                     SKYRNIMKMNLNEE"
     gene            11098..12579
                     /locus_tag="VFD89_10195"
     CDS             11098..12579
                     /locus_tag="VFD89_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HZJ58587.1"
                     /translation="MNNDQRLEFKGGMVVAFLPVCIFLFFCVLYFIVFKAFEMHALAM
                     GAFVALLVGAIFTKKGHYDGFWDAVYEGTKEAVPVAVLLFTIGIFAELIKTANISSGF
                     VWLADSLGISGGLFTGFTFIAVCIISSATGSSIGTIFTCFPIFYPAGVLLGSNPAALA
                     AAIVCGGIFGDNLAPISDTTIISAGTQEYTKKPGFADVGGCVTSRLKYSLIAGIAAFI
                     LFLIFGGGGTVGAGAGEILENNMNPITLVMLIPVAVMLFVAVKTRNIYKAITVGIIVG
                     TGVGMGFGLMTFDNVISVTDGMPTGFLTDGISGMMSTATLVLSVYGIMGVLNGAGVLE
                     KITGGILNSRMGKTVRGAEIAMMLGISITTLVFGGVTSASMTTFGKVQNEIGKRVGLH
                     PYRRANLLDGFANAIVLAVPFLSVFVFIGALLTKGYDFVEPLSITQISANMFYCLTLF
                     LVLLFSVITGWDRKFEGPNGEPITRDQGIENSAGAEAEGELLG"
     gene            12726..14954
                     /gene="pflB"
                     /locus_tag="VFD89_10200"
     CDS             12726..14954
                     /gene="pflB"
                     /locus_tag="VFD89_10200"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HZJ58588.1"
                     /translation="MNPWRNFAEGYWQSEIDVREFIQSNYTPYGGDDSFLAGPTYRTK
                     KLWKRTTELLDEENRRDGTLDIDTRTVSTIISHGSGYIDKELEIIVGLQTDEPLKRAI
                     MPFGGIRMVKNACDAYGYKLDPEVEKAFTEYRKTHNQGVFDAYTPEIRRARKAGIITG
                     LPDAYGRGRIIGDYRRVALYGVDRLIQEKKRQLGLLETAWMEDEIIRQREETTEQIRA
                     LEALKVMAKGYGFDISQPAKSAYEAVQWTYFAFLGAVKEQNGAAMSLGRVSTFLDIYM
                     ERDMAEGALTEEGAQELIDHFVIKLRLVRFLRTPEYNELFSGDPTWVTEAVGGMTLDG
                     RTLVTKTSYRFLHTLTNLGPAPEPNMTVLWSLDLPENFKNYCARMSIATSSIQYENDD
                     IMRPMWGDDYSIACCVSAMRTGKQMQFFGARSNLAKALLYAINGGRDEQLGMQVTPHF
                     APITSEYLEFDEVMERFDEVMEWLARTYINALNIIHYMHDKYSYEALEMALHDMDILR
                     TMACGIAGLSVVVDSLSAIKYAKVRPIRNDSGLAVDFEIEGDFPMFGNNDERADDLAT
                     SLVESFMRKLRKQRTYRDSKATLSILTITSNVVYGKKTGNTPDGRKAGDPFAPGANPM
                     HGRDKKGAIASLSSVAKLPYRYAEDGISYTFSIVPKALGRDKTTRENNLVAMMDGFFH
                     KKGHHMNVNVFERETLLDAMEHPEKYPQLTIRVSGYAVNFIKLTREQQEDVIKRTFFE
                     GI"
     gene            14960..15700
                     /gene="pflA"
                     /locus_tag="VFD89_10205"
     CDS             14960..15700
                     /gene="pflA"
                     /locus_tag="VFD89_10205"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HZJ58589.1"
                     /translation="MGIRGRIHSIETMGTVDGPGIRYVIFMQGCPLRCIYCHNRDSWD
                     LRGGKEITVGDLVEDINKYMIFMKGSGGGVTVTGGEPLLQPEFIRELFFKVKEMGLNT
                     AVDTSGFIDTDRIGGLLEVTDLVLLSIKHVVEERHREITGVGTRKIIAFLDHLREIGK
                     PVWIRYLIIPGYTDDPEDLNRLAKMLKDYNNVELVDILPYHDMGAYKWGELGYDYPLK
                     DVSVPTSHDMKEAKDIFRGYGLPMGDDN"
     gene            15873..>16019
                     /locus_tag="VFD89_10210"
     CDS             15873..>16019
                     /locus_tag="VFD89_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-active protein"
                     /protein_id="HZJ58590.1"
                     /translation="MLSELIKNGFGEEEGLNCAEKILYGANVAYKLGLDKEALKLSAA
                     FGGGM"
BASE COUNT         4693 a         3118 c         4029 g         4179 t
ORIGIN      
        1 attttagggt acatgcccca acagcaggat ttttataatg attttaccgc taatcgtttt
       61 ttgtggtata tgtgtgccct aaagggcatt ccaaggcaga gagccggcga aatggttgat
      121 tatgtttcga atctcgtagg ccttggggat gcccttcata aaaagctggg agcctattcc
      181 ggcgggatga aacagaggat cctaattgcc caggcgcttt taaatgatcc gaagatcctt
      241 atccttgacg agcctaccgc cgggcttgat ccaaaagaac ggattagaat acgaaacttc
      301 ataagcgaga tcgccattaa taagatagtt atcctggcaa cccatgtggt ttctgatgtg
      361 gagtttatag cgagggaggt catgctcctt ggtgaaggca ggcttataat taaggacata
      421 cccgctcgca ttattgacaa tatggtgggc agggtattcg aagctaatat tcgggaagag
      481 gagataaagg agtatcaaaa aatatacaag ctaagcagta tatccggtga tagagaaggc
      541 gtccgtatcc ggtatataac agatggagcc aaacccggcc taggggcaat accggtaaga
      601 ccatccctgg aagaggtgta tctagattat tttggttagg tgatagttct gattgaacct
      661 caaaattagg ggtatatata gcatgactat tacagcattt gttattaata catttttggc
      721 caaagatgtt taattagaat taatatgagg atgcaatcaa acaggcctct atcattagga
      781 gggagcaata ttatggatag taagaaaaag ggaagaaaag tagaggacgg acgcaatttt
      841 gaaccaaagg aaaatctgac tgcagttaac ggcagaccca tagcccataa cgagaacatt
      901 aagaccgccg gtgtccgtgg cggggttatg ctggaggata cttggtatct tgagaagatg
      961 agccatttta atcgggaagt ggttcccgaa aggcgtatgc atgcaaaagg ttcgggtgct
     1021 ttcggtacat ttacagttac taacgatatt actaaatata caaaggccga tctattttct
     1081 gaaatcggaa agcaaacgga gatgttcgca cgcttttcaa cggtagctgg ggagagagga
     1141 gccgcggacg cggagaggga tataagggga tttgccctta aattctatac agacgagggc
     1201 aattgggatc ttgcagggaa caatacaccc gttttcttct ttcgagatcc aatgcttttt
     1261 ccggatctga atcatgcagt aaaaagggat ccccgcacta atatgcgctc tgcccaaaat
     1321 aattgggatt tttggagcat gatccccgag gccttgcatc aggtgacgat taccatgggg
     1381 gataggggaa tccccaaaag ctataggcat atgcacgggt ttgggtccca tacattttct
     1441 cttataaata aagataacga acgtgtatgg acaaaattcc attttgtaac ccagcagggg
     1501 aatgaatacc tcacggatgc ggaagcagaa gaggtcgtgg caagggatag ggaatccaat
     1561 caaagggatc tctacgaagc aattgaaagc ggggagtatc ctaagtggac catgtacata
     1621 caggtgatga cagaggagca ggcacaaaat atgccctata atccttttga tctgacaaag
     1681 gtatggtacc ataaagactt tcccttaatt gaggtaggat attttgaact aaaccgcaac
     1741 cccgaaaatt attttcaaga tgtggagcag gcggccttta atcccgctga cgttgtcccg
     1801 ggaatagggt attcgccgga tcgtatgctc caaggcagat tgtttgctta tggggacgcc
     1861 cagagatata gacttggtgt caacttccat cagatcccgg tgaaccgtcc caaattcgag
     1921 gctaactact atcatcgtga cggatcaatg agggtggatg ggaactttgg cagtacacta
     1981 cattacaacc ccaattccta tggtcaatgg aaggatcaga atgaattgac agaaccatcc
     2041 tatgatggag ggggtccggc agataactat gacttccgta atgatgacga taactactac
     2101 gaacagcccg gcaaactctt taggctcatg agtgatgatg aaaagcaaag gttattcgaa
     2161 aatacggcac ggaatatgac gggaactaca gagttagttc aaaaacgcca catcaggcat
     2221 tgctatctgg cagacagcgc ttatggcgag ggagtagcaa aggccttggg aatcgatata
     2281 aatgaagtgg atatggatga cacgtatggc gcacgtgcat aggatatcat aaaggcttgt
     2341 gtgacagatg gggacgttcc ctatctgtca cattttgtat actaatcgtt ccggttcaat
     2401 ttcaatatat acaaatcctt atatatgaac acatttatat gaatcaatac gtatatgcca
     2461 aatgaggaat gtccctattt ggcacatcat gcatattgac atttgggcta aaaagtagta
     2521 gaaatatcag tataggtaaa aattatacgg ggtatagatt gaaaggggta tataaggtgg
     2581 aggcaataca cgaggcacta ttcttaatta ttgatctttc tgtactgttt tttgaaatga
     2641 tcggtgttat tttgattata gttaccggtc taaggggagt atatatttta tttatcaaga
     2701 aaaagactaa acatcactgg ctaaaatcat tgttaacagc actaagtttt ctattgcttg
     2761 cagaggtttt agaaacggta aagatccgta caataaatca actcttcact gtaggtggtc
     2821 tgtttggatt gagagctatt atggtattat atattgattg ggaaagaaag catgatgaac
     2881 ataaggaaat ccaaattcaa caatatggat gatatttcag accgaattat gaaacgatat
     2941 agaaaattgc gcatgaatag caactaaaaa gcccattatg gcgattgctt ttgttgtaca
     3001 ataaatgtat agggactaaa ttcctaaccc ctgaatttat tggacacagc cattctttta
     3061 aagattcgaa cataaaataa tggctagcca ataaaaatcg ccatatcaat gggagataat
     3121 caagaaacta ttgtttcttg acagtcggcg gggttctaag gggtatgtca ttttcaaaca
     3181 aaccggggtt ctcccttaat agacccataa cccaatccgc cataggtttc acctcaacaa
     3241 cataggaaag agcatctccg attcttgctt ctttggctat ccccatacgc agcataggag
     3301 tcaggagctt gtcatagttt tgtgacacat ccccattgac aaaagaatga ccataaaaat
     3361 caattttccg aatactgccg tcttcgagga gcaatttaat ttgtctcgac tgtgggttga
     3421 tacgattagg tatattcaac cattcctcaa ggctatgaat aaacgtgttg gtcttaaggg
     3481 atgcgcccaa aaataacaac tgcgcattct catcgtatag cctgccgaaa cagccatgtc
     3541 tcgggcaggg cgtatgaaca tcgctatcat gaagaacata ggattgggct ctggccccta
     3601 tagccgcaac cgaatgggta ggatgcattg atcgaataac cccggtcctt ttcaaaaaga
     3661 ggtttggcaa caatcccaca caggccggct cttttattgg atcatagaca tcgtctggca
     3721 gattatcctc gccccatgaa tgggtaggaa ataacaatag tccatatttc atatgctcga
     3781 taaaggaatc aaggaccgta tcagccccac cttccacggg accaatagct ttcatagagg
     3841 aatgaatgag caacgttccg tcctttctaa ttcccgcctg ttcaatatct ttttttaaag
     3901 atcttttagt gtgcatgtat agatattcct attctataat ttttatcgtc ttgacgacga
     3961 tcaatatgat attactacaa aaatgggaaa aagtcgattt ttatgagacg gtttatcggt
     4021 taggataaaa atgcattatc ccagctaagt tttacacgat cattgtttta ggttacatgg
     4081 gcgaaaagcc cgggatagaa tctaggtatg aacttatgcc caatatttta gatgggatat
     4141 ccccgggact tgattctgat ttaggcaatg atatataata gaacatatag gcattctata
     4201 tcctttatat gattaaaacg agtcggcaag atatcccccg cttctataag cggtgagttc
     4261 cgattatagc aaagcatgcg gtgggtataa atcccccgtc tcgttgaaac ctgtgtgatg
     4321 taattgccaa tgcaattgct tccgatgtta taacgaatta cagaaccctg gaaagataat
     4381 aatgggagag ggatagcgtg tataaagatc ataagataat agccctatgt attacacaac
     4441 tccatgatga aagaaatttt gattttgtct ccgccttgaa caaaaaaata aatgattttg
     4501 gatacaaact ctttatctac cactcccact tggattttta tgacgagaga caaaacgaaa
     4561 agggcgacga agcagtattc gaccttatag attttgatat agtggatgct gtaattattt
     4621 acgaggaatc cataagaaat caaaggattt ctgacggcat tgtaaaaaaa gctaaatcga
     4681 aatccgtccc agtaataact attggcgtta cgaagcagag cactgcaaat tttattttaa
     4741 accttgaaat tggatttgaa aaggttgtac gccatgttat agaatttcat aagataaaag
     4801 atactattat gattgcaggg cgcagagaca gttactattc caacaaaaga attgaggtct
     4861 acaaaaaggt tctctcagaa aacaatataa gatttgatga ttccatgctc tattacggtg
     4921 atttttggtc ggagccgaca tgtgcggtta tgcaggaatt gattgacaat aaccgcctac
     4981 caaaggcgat aatatgtgca aatgatggca tggcaattgc cgccatcgac accctacatg
     5041 aaaacgatat ttttgttccc gacgatattg tcgttacggg atttgatggc ataagggatg
     5101 caaggtataa tatcccgcct gtaactacct gctcaatgaa ttatgagaaa ttggcatatg
     5161 atattgtttt gtgtatagaa agattattaa gtggtaaaaa gattaataaa gaaaactata
     5221 cagatcagac aatagaggtt tactcctcat gcggctgtaa caaggtgcct gatatggaac
     5281 acgggggctt attgaggatt gtacgggaca ggtataagag gtttctccat tataacgacg
     5341 aacttcacga aatgacagag cgtgtatttg attcaaagac ccccgaagag attcctcgga
     5401 ttttagaaca atatgaactg tatgattgct atgtttttgt taacgatttc ttttttgata
     5461 aatcgattag gctgacagag gatataaagg gaaaagcatt tagtgacaaa ttatatttac
     5521 tctataagac cggtctggat gctgataact atccgatgga atttcagagg aaggatattc
     5581 taccggatat agacagtctg atccaggaga atgatcccat tatcttcaca tccctcagtt
     5641 ctagggggct tccatttggg tttttgtgca ctgcctttaa aattgatata gagaattata
     5701 gctttttacc ccaatttaca gtatttttga gcaatgcgtt gagcggatat agaaatattc
     5761 gctatttaga ggatctatcc aagcgatatg aaaaacttgc ggaagaggat tttttgacgg
     5821 gacttctgaa taggaccggt ttttataatg aaatcagcag gctaaaggaa accatagaca
     5881 ccactgttac tgagaacttc atagtcaccc tagttgatct ggacgggcta aagcagatca
     5941 acgacaatca tgggcatcat gccggagacc aagcaattat taccatagct aatattatta
     6001 agaccatacc cattgaaaat aaaatctgtg tacggttggg cggcgatgaa tttttgatat
     6061 gttctttgtg tacagataag gaatccacta aaaaaatcat aagaaaccat ctctttgata
     6121 acctgaaagc tatcaacaaa tattcggcta acccctttaa gatttccatt agcataggtt
     6181 tcagttcatc cggaagcgga aaaatcgata tagacaacct cttaaaacag tccgatgaat
     6241 taatgtataa tcataaattt gagaaaagac aagggcgaaa tacgagatat cattaggtct
     6301 gatcaatgtg attaggccga attatcattg ttataacgag ggggggtacc taatgaacaa
     6361 agaaaaattt ctttcgtgca tccaaaatga accaattata aactcccatt cccaccattt
     6421 acaagatgtg gatcatcggg ggctaaatct ccaaaatgtt ttgcgtagct cctatgtcaa
     6481 ctggtgtcat aaacctgttc ctgatgggaa ttctaaagat gagacagccc tatggctgaa
     6541 ttctgtcaga aaccgcagct attttatatg gatagaaaag gctatgatgg atctgtatgg
     6601 tatagatgag catttagatg cggatacatg gtatatattc gatggagcca tacgcaaggc
     6661 gcatagcggg ggagcctggc atttaagcat attaagggat caatgcaatt acagagctat
     6721 ttttcttgac actttttgga agccaggcga cgacaataat catccggact tgtttaagcc
     6781 ggcgtatcgg gtcaacagcc ttttttatgg ctataatcgg acagcaaggg accataacga
     6841 aaacaatttt caactgttag aggaccgatc cataacggat atcgatgaat atataaaact
     6901 tattggggat gtaatacata gaaagagaga ggccggctgt accgttataa aatgcgccct
     6961 ggcatacgat cgctctctgg gatttggaga cgggacaaag gagaatgcac aaagagctat
     7021 gaatgagaat cccgacgagg atgacattag ggcattccaa aattatgtat tcgataggat
     7081 atgtgagatt gccgcggaac tatccatgcc tgtacaaatc catacaggtt tggggttaat
     7141 ggccgggagc aacgctatgc aattacagcc gttgatctct cgaaatccga atacgatatt
     7201 ttggcttatg cacgggagct atccatggac gagcgatatc gcgggattgg cccatgtata
     7261 ccccaatgta tgggcggatc tgtgctggct gcccctgata tcgccgactg ccgcacatcg
     7321 tctgatgcat gaactcatcg atgtatgtaa cgctgatcgc gtagtttggg ggtgcgatac
     7381 ttggaccggc gaggagagct acggtgcgcg tttggcattc ctggagatat tatcacaggt
     7441 tctttgtgaa agggtggata tggggagaat gcgggaaggc gatgcccaaa gatatgcaag
     7501 ggccgtcatg tatgataatg ccgccggcat actgggtgac atttgaagag ggtaaaagca
     7561 ttcggaataa atactgcggc ggatatatac ggactttacc ggactgaaac ttatatgttc
     7621 gggtttgtat acgggaacat ttttttagat tcccatctac gaaatgaggt ataaaatgag
     7681 aaagacaaaa attatttgta ctctggggcc tgccagtgac gatcccggga tcttaaaagc
     7741 cctgttagag aagggggcta acgcaatcag attgaatttt tcccatggcg atcacgaaga
     7801 acatgggaaa cgaatagctc tggttaagca agtaagggag gagatgggcc ttcccatagc
     7861 tatccttatg gatacaaagg gtcccgaaat ccgcatcggc aacttcaaag acggggaagt
     7921 cagactggag gaagggcaga agtttgtctt gactacggag gagatccaag gggacgagaa
     7981 catagtcacg gtccgtttta aggaaataac caaggatgtc tgtgtaggga ccgccatact
     8041 tatagacgac ggcctggtcg ccctggtcgt ggaggaaata acgcaagatg cggtccattg
     8101 taaggtgata aacgggggta tcctaggcag cagaaagggc gttaatattc ccggggtttc
     8161 cacaagtctg cccgctatta cgaccaagga cgccgaggat attaaatttg gcatatccca
     8221 gcagatagat tatattgccg cttccttcgt acggagggct tcggatgttt tagccataaa
     8281 gagggtttta tcagagaata acggtgatta cattaaggtt atcgccaaaa tcgaaaacca
     8341 ggaaggcctc gaaaatatag acgaaataat aagggtttcc gacggaatca tggtggccag
     8401 gggggattta ggggtggaaa ttcccacgga ggatgtacct atccaccaaa aggagataat
     8461 caaaaagtgt atcgaggcca gcaaacccgt catcattgcc acacagatgc tggattccat
     8521 gatcagaaat ccaagaccca cacgggcaga gaccagcgat gtagccaatg caatctatga
     8581 tggggccgat gttatcatgc tgtccgggga aaccgccgca ggcaagtatc ctgtagagac
     8641 gctgctgact atggccagga ttgccaaaag ggtcgaaaac agtatagact atgaaatgga
     8701 atttgaaaaa cgcgtagccg gcaaggaagc caccgttacc gattccataa gccatgcaac
     8761 gtgttcaata gcacacgggc tgcatgccaa agccatcgta acagctacca gaagcgggcg
     8821 taccgccaga atggtatcta aatatcggcc tgctgcccag attatcgcta ccacaaccag
     8881 cgacgcgacc tgcagggagc tttccctggt atggggcgtt cagccgtttg tttcgccgga
     8941 tatgagcgat acggatagga tgtttgaaaa atccacagag gttgctgcca ataccggtat
     9001 tgcatgttcc ggcgatatca tcgtaataac ggcagggata cccatgggaa tcagcggttc
     9061 gactaacctt attaaggtga gtcagatagg ggatgtcccg gcgggaaatt aattgatgaa
     9121 aaaatttact aagttggggc ttgacagcct aattcacagg tggtatattc atataaaccg
     9181 aagacgaggg agttatgccg ctaaaacgat cttcagcgaa tccgggacag tgtgagccgg
     9241 acagatgaca gtggtggcaa gtgggcctcc gagggcaagg cgaaagattt attcagtagc
     9301 taagacgcag agccggcgtt atagggctgt gggtgtaatg gcaccctaca aagtgggtat
     9361 atattttata tatcaaacaa ggtggcaccg caggttagcg cttgtccttg agtattctca
     9421 aggcaggcgt tttttatatt cctaaaaaca atgatgggcc attagcctat actgacatag
     9481 gagttgttta gattgattag ggaacattga tcgtataatt gattgcataa cttcatagat
     9541 aaccgactcc gactataaaa tttatcatct gcagacaaat ttagtttccg tattgtctat
     9601 tctatagatc aacttaaccg aagaaaacgg atttaatcac aaaggacccg gaggggcccg
     9661 gaaggaggga ttggagtatg ggggataggt ttacgctcta taacttaaag gtggaggttg
     9721 tttcatcgga caagcccatg gtgtgccggc ataaggaggg tgactacttc ttggtggaag
     9781 gggaggattt aatctttccg gagaataatt ccttctccat gtatgccctc agtgcgattc
     9841 tgcctttgct tcctgcaaag cagcgcccgc tgcacccaaa cgattggatg ttgtccgaca
     9901 gtatgatagc ctgtccggac cccaattgcg gtgcgaaatt caagatcacc cgcataggca
     9961 caaaggagtt cagccatgcc gagtgcacgg tagaaaaaat atatgatggg gaggaagagt
    10021 aatggtccaa agagttgagc tttccgacgg ttatacaatt agcagggtta tcaacggttg
    10081 ttggcagttg tctcaaggac ataatcttat cggcagcctt gatatgaaag atatcatgcg
    10141 tgcattccat atgctggccg aagggggatt caccaccttt gactgcgcag atatctatac
    10201 gggggccgag gaatttttag gtgagttcat aaaggagctg agaaggggca gcggaatttc
    10261 gtcccatgat attcagatac ataccaagta tgtgcctgat atcaacctac tggcccaggt
    10321 ggattatcaa tataccgaag ggatcataga ccgcagtcta aaacgactaa acagggacgt
    10381 attggatctg gtgcagtttc actggtggga ttacgatgcc ccgggctgtc tggagactgc
    10441 aggctatcta ctcaggctaa aggaaaaggg taagatccgc aatataggcg tgaccaattt
    10501 tgacaccgag cattttgcga acatagttga agccggtatt cccattgtat ccttccagac
    10561 acagtattcc gtctttgaca ggcgcccgga aaaggctcta acagagtatt gcagactcaa
    10621 gaatatttcc ctgctctgtt atggtaccat agcgggggga ttcctggcag ataggtggat
    10681 agggaagacc atggatgtcc ccgaaacccg ttctcaggtc aaatacctcc aagttatcga
    10741 agacacctta ggttgggacg ggtaccagga cctacttgca ctccttggca gaatcggcga
    10801 taagtacggt gttaggattg cccatgtggc ggtaaaatac attctcagtc aatctggggt
    10861 gggcgcagca atcatcgggg taagaaatag caggcacgtt caatcgaata cggggatatt
    10921 tgaatttgat ttagaccatg aggagatcga tgagatcaga gactttttgg accggtatcc
    10981 gatattagac ggtgaaccat ttgagctgga gcgcaccgtc ggttccaagt ataggaatat
    11041 catgaaaatg aatttgaacg aagaataaaa tctattattt taaggggagg gcccgatatg
    11101 aataatgatc aaaggcttga attcaaggga ggaatggtcg ttgcctttct tccggtatgt
    11161 atctttttgt tcttttgtgt tctgtatttc attgtcttta aagcatttga gatgcatgcc
    11221 ttggctatgg gtgcatttgt cgcgttattg gtcggtgcga tttttacaaa gaaaggccat
    11281 tacgacggat tttgggatgc tgtctatgag ggtacgaagg aggccgtgcc ggtggcggtg
    11341 ttgcttttta ccatcggtat ctttgcagaa cttataaaaa ccgctaatat ttcctcgggc
    11401 tttgtatggc tggcagattc tttggggatc agcgggggcc tgtttacggg ctttaccttc
    11461 atcgcggtat gtattatctc ctccgctacg gggtcgtcca tcggaaccat attcacctgc
    11521 ttcccaattt tttaccctgc aggggtcttg ctgggcagta atcctgcggc tctggcggct
    11581 gctatagttt gcgggggtat ctttggagat aatctggcac ctatttcgga tactaccatc
    11641 atatctgccg gtacccagga gtatacaaaa aaaccgggtt tcgcagacgt gggcggttgt
    11701 gtgaccagca ggctaaagta ttcattgatt gccggcattg cagcgtttat tctgttttta
    11761 atctttggag gcggcggaac agtgggagca ggggcggggg aaatcctcga aaataacatg
    11821 aaccccataa ccctggtgat gttgataccc gttgccgtta tgctgtttgt ggccgttaag
    11881 acccgaaata tctataaggc aatcaccgtg gggattatcg tcgggacggg tgtcgggatg
    11941 ggatttgggc tcatgacctt tgataatgtc atatccgtta cggacggaat gccgacgggc
    12001 tttctgacgg acggtatcag cggcatgatg tctacggcca cactggtcct ttctgtatac
    12061 ggtattatgg gtgtactgaa cggcgcaggt gtgctggaaa agatcaccgg cggcatccta
    12121 aacagcagga tgggcaagac cgtccggggt gcggaaattg ctatgatgct cggcatctcc
    12181 ataaccaccc tggtttttgg cggggttacc agcgcatcca tgacaacctt tggaaaggta
    12241 caaaatgaga ttggaaagcg agtaggtctt catccatatc ggcgtgcaaa tctgttggac
    12301 ggattcgcca acgccatcgt attggcggta ccctttctga gcgtgtttgt atttatagga
    12361 gccttgctca ccaagggata tgattttgtc gaaccccttt caattaccca aatcagcgcc
    12421 aatatgttct attgtttgac gctgtttctg gtgctgttgt tctctgtcat aaccggttgg
    12481 gaccgcaaat ttgagggtcc taacggagaa cccataacaa gggatcaggg aatagaaaat
    12541 tcggcggggg cggaggcaga aggggagtta ctgggataac ataatatggg gggcgaaagc
    12601 ccccctccta tttttgagta ttggatggcc ctatgccttt agctgcaaga agaagattta
    12661 ggttgaggat aattgattca ggttatatta tagttaaagc agatgataag gagctgattt
    12721 ataatatgaa tccatggaga aattttgcag aaggctattg gcaatctgaa atagatgtta
    12781 gagagtttat tcaatcgaac tataccccct atgggggcga tgattcattc ctggctggtc
    12841 ccacctatag gaccaaaaag ctatggaaaa ggaccaccga gctactggat gaggaaaata
    12901 gaagggacgg aaccttagat attgataccc gaaccgtatc cactattatc tcccacgggt
    12961 cgggatatat agataaggaa ttggaaatta tagtggggct tcagaccgat gagcccctaa
    13021 aaagggctat tatgcccttt ggcggcatta ggatggtgaa aaacgcctgt gatgcctatg
    13081 ggtacaagct tgacccggaa gtggaaaagg cttttacgga atatcgtaaa actcataacc
    13141 agggggtttt tgatgcctat acccctgaga taagaagggc caggaaggcg ggaatcataa
    13201 cggggctgcc agatgcctac ggcaggggca gaataatcgg cgattatcgc cgggtcgccc
    13261 tatacggagt ggatcgactt atacaggaaa agaagagaca gctgggtcta ctggagacag
    13321 cttggatgga ggacgagatc atccgccaac gagaggagac cacagaacag ataagggcct
    13381 tggaggcttt gaaggtcatg gccaagggct acgggtttga tatttcccaa ccggccaaaa
    13441 gtgcctatga ggcggtgcag tggacatatt ttgccttttt aggtgcagta aaggaacaaa
    13501 acggtgcggc tatgagcctg ggaagggtgt ctacattctt ggatatctat atggaaagag
    13561 atatggctga gggagccttg acagaagagg gggcccagga attaatcgat cattttgtta
    13621 tcaaattgag gttggtgcgc tttttgagga cgcctgaata taatgaactg ttcagcgggg
    13681 atcccacctg ggtgacagag gccgtcgggg gtatgacctt ggacgggagg accttggtaa
    13741 ccaagacctc ctatagattc cttcacaccc taaccaacct agggcctgca ccggagccca
    13801 atatgacggt tctatggtcc ttggaccttc ctgagaactt taaaaattat tgtgccagga
    13861 tgtccattgc aaccagctct atacagtacg agaatgatga tataatgagg cctatgtggg
    13921 gtgacgatta ttctatagct tgctgcgtat ctgccatgag aaccggaaag caaatgcagt
    13981 tctttggggc cagatcaaac cttgccaaag cccttctata tgccataaac gggggaaggg
    14041 atgagcaact ggggatgcag gttacccccc attttgcgcc gattacctcc gagtatttgg
    14101 aatttgatga agttatggag agattcgatg aggttatgga atggctggcc aggacatata
    14161 taaacgccct caatataatc cattacatgc acgataagta cagctacgag gccttggaga
    14221 tggccctcca tgatatggat atcctccgaa ctatggcttg tggcatcgcc gggttatcgg
    14281 tggtggtgga ttccctcagc gctataaaat atgccaaggt aaggcctata agaaacgatt
    14341 ccggactggc tgtggatttt gaaatagaag gcgattttcc catgttcggc aacaacgatg
    14401 agagggcaga cgaccttgcc acatccttgg tcgaaagctt tatgagaaag ctgagaaagc
    14461 agaggaccta cagagacagc aaggctaccc tgtccatact gaccatcacc tccaatgtgg
    14521 tctatggcaa gaagaccggg aatacccccg acggaagaaa ggccggagat ccctttgcgc
    14581 ccggggccaa tcccatgcac ggcagggata aaaagggagc tattgcatcc ctcagttcag
    14641 tggcgaagct gccctatagg tatgccgaag acggcatttc atacaccttc tccatagtgc
    14701 ccaaggcctt gggccgggat aagacaacta gagaaaacaa cctagttgct atgatggatg
    14761 ggttcttcca taaaaagggc catcatatga acgtaaatgt ctttgagcgg gaaaccctgc
    14821 tcgatgccat ggagcatccg gaaaaatatc cccagcttac cataagggtt tccggatacg
    14881 cggttaattt tattaagctg accagggagc agcaggagga cgttataaag cgcacattct
    14941 ttgaagggat ctgaaaagca tgggaataag gggtagaata cactccattg agaccatggg
    15001 caccgtggac ggccctggca tcagatatgt tatctttatg cagggctgtc ccttaaggtg
    15061 catctactgc cacaataggg attcttggga tctcaggggc ggcaaggaga tcaccgtagg
    15121 ggacttagtg gaggatataa acaaatatat gatttttatg aaagggtcag gcgggggtgt
    15181 caccgtaacc ggcggtgagc ctctgttgca acctgaattt attagagaac tgttttttaa
    15241 ggtaaaggag atgggcctta ataccgccgt ggatacctcg ggttttatcg acacagacag
    15301 gatcgggggc cttttagagg ttacggatct ggtgctcctc agcataaagc atgtggtgga
    15361 ggaaagacat cgggagataa ccggggtggg tacccgaaag ataattgcat tcttagatca
    15421 cttaagggaa ataggtaaac ctgtatggat cagatatctc attatacccg gttataccga
    15481 tgatcctgaa gatctcaata ggttggccaa gatgttaaag gactataata atgtagaact
    15541 cgtcgatatc cttccttacc acgatatggg ggcctacaaa tggggggaac ttggctatga
    15601 ttacccctta aaggatgtat ctgtgcccac aagccatgat atgaaggagg ccaaagatat
    15661 atttaggggt tatggtttgc ctatgggaga tgataattag gccccatgga aacaatttat
    15721 cctagaataa tccacctgct ccaggtatag ttatttatct tatccacccc atatgttata
    15781 atcatgacaa gccgatacaa gttttaatta tgggatcagg cgggctttgc catgtgtcct
    15841 tatcggataa aattgaaagg gtgggtactt atatgttgag tgaacttata aagaacggat
    15901 ttggtgaaga ggaaggccta aattgtgccg agaagatctt atatggggca aatgtcgcat
    15961 acaagctggg tcttgataaa gaggcgttaa agttgtccgc agcttttggc ggtggaatg
//
LOCUS       DASRNX010000256        17338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_740550,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000256 DASRNX010000000
VERSION     DASRNX010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 17338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17338
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740550"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..975)
                     /locus_tag="VFD89_10215"
     CDS             complement(103..975)
                     /locus_tag="VFD89_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HZJ58591.1"
                     /translation="MMKLKSLLAITLLVLLIFLSACHRPAPAPDNKLTVAVSIVPQAT
                     FVEEVAGDLVEVITMIPPGGSPETYSPTPRQLEAFTRASAYFSIGVPSEVSNILPRAK
                     DLNRDLEIIDLAKDVSESYPDIEIAPGERDPHIWLSPKRVKVMVERIADELSDLDPAN
                     KDVYNENALKYKERLDDVDKRIAESLDGLETKTFIAYHPAFGYFAHDYGLRMLALEEE
                     GKEATPQTFKDIIDIAKEMKIEVIFYQAEVDSRQSEAFAKEIGGRTEQIAPLAPDYIE
                     NLERMADTFSKVLR"
     gene            1457..1879
                     /locus_tag="VFD89_10220"
     CDS             1457..1879
                     /locus_tag="VFD89_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZJ58592.1"
                     /translation="MEDTYITCHRKLMELAWRIRGRSRCNLSSLGFAWNQYSIMKALE
                     PEEGLTVSEISTRVHRKISNVTPVVDFLVDKGIAERISDEKDRRVIRVRLTAEGIDIR
                     QGAIDHHHDFLNSLYQSLTEEEMGIFLDIVNTFMEKVK"
     gene            1941..3707
                     /locus_tag="VFD89_10225"
     CDS             1941..3707
                     /locus_tag="VFD89_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58593.1"
                     /translation="MNKKSFLFKYLAKHKLRYVKGLFLLIIINFLQVLVPRITGQVVD
                     GLESLSLDSRKLLYYGGILILISLMTFIAHFLSRLQVAGASNLFDYEVRNGMFDHMLK
                     LSMKFFHRNRVGELMALATNDLGSLRMILSRGFNLMTNTVILLISSIVIMVKYVDPRL
                     TLYSFLPLPILVFIMTKFGPMINRRFRRVQESFSDVTRKTTENITGIRVVKAFVQEDY
                     EKENFAKVNQENFDINMNLVKLQGIFYPLINVISSLSFLIAFIYGGNLIITNQISLGD
                     FIAFNGYLGIIIRPVMSIGMIINFTQRAKASVERIEELFDEKPEVVDPAALNPGKEHI
                     EKDFYGRIEFNDLTFSYGKTEPDVLKNIDLVIEPGKTLGVIGKVGSGKSTIVNLILRL
                     YDPAEKGQLLIDGEDITETSLKRLRDNIGYIPQDNFLFSSSIRENIGFSPNEPTLDEI
                     RGAAAMSQIDESIMELSDQYETMLGERGVNLSGGQKQRVSIARALVKDPKILILDDCL
                     SAVDTDTERKILNELKPFMEERTCVIIAHRISSLQDADEIIVLDDGRIIERGSHEELL
                     EQQGYYNRIYQRQLLEEEIEIA"
     gene            3794..5539
                     /locus_tag="VFD89_10230"
     CDS             3794..5539
                     /locus_tag="VFD89_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58594.1"
                     /translation="MLEFAIPYWHFFVIALLLILTITGASLARPYLTKVGIDKYINGV
                     LKNTMTIDEASRGIWVLGIFFLVLIIVEFALGYLQRFILEYTGKKIIYNIREKIFNHI
                     QHLPLSFFDKMAAGRIVTRVTNDPETINEMFSGVLVGFIKSMVEMAAIIVIMFKLSSK
                     LTLVSFTVLPLIVIATVIFRKTARKVWQRIRAKLSAINAFLAEHIAGMRIIQAFNMQG
                     KKAEEFDKTNKEYYDAHMKSVIVMGIFRPFMDVVESLGMALLLWYGGRNILAGALEFG
                     TLYMFVDYIGRFYWPIRELTEQFNTLQSSTVSAERVFNLLDEKQEQQIEGTIQGYDNM
                     EGEIEFKNVWFAYIDENWVLKDISFKIRPGESAAFVGATGAGKTSIISLLCGFYEHQR
                     GEILIDGVDIRDIGKEKLRDNIGLVLQDVSLFSGDIATNIRLFDPNVSMEEVERAARH
                     VNAHEFISKLYGAYDHEIGEGGTTLSVGQRQLISFARALIRDPKILVMDEATSHVDTE
                     TEELIQDALINLMKGRTTIAVAHRLSTIQNADKIILIHRGRIREMGSHQQLLSNRGLY
                     YNLFRLQYDPEYKVG"
     gene            5580..6905
                     /locus_tag="VFD89_10235"
     CDS             5580..6905
                     /locus_tag="VFD89_10235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HZJ58595.1"
                     /translation="MVRLRFIHTADVHLGSLLHMGGDNLPPGVKEAAITATSEGFGRV
                     CHAAIENSVDFIVISGDLYDREARSVRAMSFFAEQCRLLEDAGIPVFIIAGNHDPLKE
                     ERDLIRVPDNVKVLRGDCPETIELLGPDGNPMARVIGQSYQSGSLGKGIHLDYKVPDG
                     DIWNIALLHTQLETPRSNYVPCSTAELKEIPHIHYWALGHIHKYRRLSNDIPYIAYPG
                     IPQGRDFGETGRGGCILADLDPFGESHISFMPISPVIYKRIDILIDDDLENTPETLQD
                     LLDKICMEGDKILRETGEDEDYPVRGYVVEWTIRGRGAIHSLLKQQEQESIEFLLDSL
                     REEYGSFDPFLWTDAVTLRTQSPIDYTGLLENSPVFKELDRIIGQCLKDEGMRERLMD
                     ELGIIWRGSGDHEAEDDLRFHMDDASLEEVLQRARYMIAEKLVERGEQP"
     gene            6902..9784
                     /locus_tag="VFD89_10240"
     CDS             6902..9784
                     /locus_tag="VFD89_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZJ58596.1"
                     /translation="MKIQRLYIGDFGILRNQTLDELGPGIVVIGGLNRAGKSTLMHVL
                     RHLGYGFPRDSNLPPANSRYEAEADIRLGAGDVYNLRLKGYGEPILKRITGLETDISA
                     PKDLYGLDSFSYNRLFTITLDELSINRGISDEDKMRLQSILLGAGFSEMLLLPQLERD
                     FYREGDSIGGKRGNPGVKQFRPYSSIIEAGRKLKQRGLSQIGEYQDKQQELQHMDELA
                     EALNNDIKELDNLIVQLDIIKNSYGSYLRVQELKTWLDVNKDQEWEDGPREHQLERIE
                     SLREEYIGLCRKLREKELQSGLTNRLEESLLGVKEEMADLLAGISGVYERMRGLGQRK
                     QELDRKRRDIILKAGEANAHWGETDIDYITRIKADNVEHNRLNELVEEYRELEYRKRT
                     QADILQRAREEAQAFKDRTAEDNDGRPWIGIKKYFYFSLILTLLGMALSMMNPLLGLI
                     VGVGGTVGAALFLIMKTLGRRTDTRAAYEQEKELRTRQAKVRAEQGILEQLESNLRLL
                     EETLNDYKAKLGLPERASHSALPGHLLRIRDIQTKAAEVDWLLGELDDTKKLIGKQYV
                     RYKKFLDQFCSGEPRSSDGRVDPCDGEEWNRISHTLKLWADRLKDAEELGLLRDEKAA
                     IRRDIMDMVGKYDPADPIPEESNARSDCSGPFDSLVLDFIERAQDGIVYAENKAFLSE
                     SIQSIAGSMSSDGIRRAFIPESADRADLLKAFIERCQEYASVEEADREYSMKMGERGA
                     KLDELDGVKEAIRKLRDDLERLGAVENLTLGQRQIDSGKAELKVLADQYAVNMTSAFL
                     LREAEKNLLEGMKDSVMKSAGIIFNRMTGGHYHGILPSEPLLESDFQAVPDGDGESQT
                     IDMLSRGTREQLYLSVRLSRIMDIKPCLPIIIDDSFANFDSIHLNQSIDILSELAKTH
                     QIFILTCHGELVGRIAESGCCAQYWKLERGKLKVTDCDELSDHLWQ"
     gene            9887..11146
                     /locus_tag="VFD89_10245"
     CDS             9887..11146
                     /locus_tag="VFD89_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZJ58597.1"
                     /translation="MDYLIRDINLAPEGYGRINWVKGYMPLLNELEREFEREKPFKGK
                     RIAISVHLEAKTAYLALVLTKGGAEVAVTGSNPLSTQDPIAAALVKEGLSVYAWHGAT
                     DDEYNQHLNRTLDFGPHIIIDDGGDLVHLLHTTRADLAGGVLGGCEETTTGVLRLRAR
                     EREGILDFPMIAVNDAYCKYLFDNRYGTGQSVWDGIMRTTNLMVSGKNVVVAGYGWCG
                     KGVANKAKGLGANVIVTEIDPIKAIEAVMDGFRVMTMDEAAPIGDIFVTVTGCNKVIR
                     EEHFIKMKDGVLLANAGHFDVEVYKPDLEKLAVEKKDMRQNILGYVMEDGRVLNLLAE
                     GRLVNLASGDGHPAEIMDMSFALQALSAKHILEQHSVMNNRVYKVPEKIDNRVAAMRL
                     SAMGINIDILTDEQEAYLKGWGSTASY"
     gene            complement(11359..12165)
                     /locus_tag="VFD89_10250"
     CDS             complement(11359..12165)
                     /locus_tag="VFD89_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58598.1"
                     /translation="MLGKLLKYETKATARSFLPVYIAILLFAFINRVINPFEQVGDTT
                     GVTIEGFSFLNMMRIASVVIYFALIVATIAMTFVIIIQRFYKNLLGDEGYLMFTLPVK
                     TWQHIISKLLVSLLWTILSFIVIVSSVLVLIRVDNLFGEIVRIIGEGRRFFGDVFLIL
                     APISSLIGSTYFILTVYNALSIGHLFTKHRILASFGAYIGIYIFTQIVLTIFIFATAN
                     AIFQPLAQSDLPIHAQILTLMISMMVLYSLLGAANFASANIILNRKLNLE"
     gene            complement(12159..12857)
                     /locus_tag="VFD89_10255"
     CDS             complement(12159..12857)
                     /locus_tag="VFD89_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZJ58599.1"
                     /translation="MSNLLECKNLTKTFGSTIALWDVNLEIGHGRIIGLLGPNASGKT
                     TFIKLCNDLLTPTKGEILIGGDRPGIRTKGVVSYLPEKTYLNDWMRASDIIEFFKDFY
                     EDFNPAKAYDMLGKLNINPKDRLKTMSKGTKEKVQLILVMSREAKLYLLDEPIGGVDP
                     AARDYILNTILSNYSEDATVIISTHLISDIEKILDDIIFIKEGQVVMNKTVDEIREES
                     GKSVDALFREVFKC"
     gene            complement(12854..13225)
                     /locus_tag="VFD89_10260"
     CDS             complement(12854..13225)
                     /locus_tag="VFD89_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZJ58600.1"
                     /translation="MHWDLTSDRPIYAQIIEQMELKICSGVYPLGSKLPSVRDLAQEA
                     SVNPNTMQRALSRLEETGLLYSLRTSGRFVTEDINMVKTTKKRLAREHVQEFLEKMIR
                     LGFDQDDIISMLGDISKELKK"
     gene            complement(13682..14167)
                     /locus_tag="VFD89_10265"
     CDS             complement(13682..14167)
                     /locus_tag="VFD89_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HZJ58601.1"
                     /translation="MRSYEFAIQMELDGERYYMEQAEKHRDSGLHTIFLTLARDERKH
                     AQILKDRMKQLDTVLSETTSYADYKNVFQDTPDFHNVIKDIPDQLDVYSVAMDKEKES
                     INLYKGMLEEARDDGDREVFRFLIHEEQIHYNLLEEIWSHVNRAVEWVESAEFGLREE
                     Y"
     gene            14596..15603
                     /locus_tag="VFD89_10270"
     CDS             14596..15603
                     /locus_tag="VFD89_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015769590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HZJ58602.1"
                     /translation="MNRYFKFFILMGIIFGAFFLIREYIPKADGIRQTPNGGHRFSEQ
                     IEENEGLSPANDEKDSPVSGDDNNDVEDNPQITSVPNNGYINISGMTIKERYSLPQGY
                     SRVSAEEGSFGEFLRNQRLKPYGEKSLYYDGREKPSAGIYDGVMDVDIGNRDLHQCAD
                     AIMLLRGEYLYSLGAYEDINFNFVSGFRAEYKKWTDGYRIKVNGNKVEYYRGADPSNT
                     HEDFRKFMDMVMAYASTLSLEKELESVHIQDLDIGDVFIIGGSPGHAVIVVDVAESAD
                     NEKMFLLAQSYMPAQQTQIIINPMDRDISPWYSLDGKDELITPEWRFSLDNLKGFRSN
                     D"
     gene            15712..16101
                     /locus_tag="VFD89_10275"
     CDS             15712..16101
                     /locus_tag="VFD89_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58603.1"
                     /translation="MAILFPEGPGGMPADALKYAPFDNYLIPGIILFSVIGLGSILSA
                     FSIQKKMKHHAYIAMTSGWALVIWIVVQCIMLRTVVALHIIFFVIGLIEVFLSGILFI
                     RQNLFPINLIRGIMSSSKKGSAELKLP"
     gene            16325..16879
                     /locus_tag="VFD89_10280"
     CDS             16325..16879
                     /locus_tag="VFD89_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HZJ58604.1"
                     /translation="MKVIVFLADGFEEVEALTVVDYLRRVDISVETISITREREVMGA
                     HDIPVLADKTIDEVGDAELYDGVIIPGGLPGATNLRDNPMVIETVKGVYENGKLAAAI
                     CAGPIVLERAGIIEGRRVTSYPGFKDQLPGSIYVEQGVVRDGNIITARGPALAVYFAI
                     EIAEYFLGEKKAEELQRAILLPQT"
BASE COUNT         4990 a         3440 c         4377 g         4531 t
ORIGIN      
        1 catacgaatt aagggtaatg ttttacgaca cctatgtatc aatgttggaa gttgcgttca
       61 atagccatta ccaatactcc gatatcacaa aaacatactc cttcatctaa gcaccttgga
      121 aaatgtatct gccatccttt ccaggttttc tatgtagtcc ggtgccaggg gtgctatctg
      181 ctccgttctg cccccgatct ccttggcaaa ggcttcggat tggcggctgt ctacctccgc
      241 ctgatagaag atgacctcga tcttcatctc ctttgctata tcgataatat ccttaaaggt
      301 ttgtggggtt gcttcctttc cttcctcctc cagggccaac atccggagtc cgtaatcatg
      361 ggcaaaatat ccgaaggcgg gatgataggc aataaacgtc tttgtttcaa ggccgtctaa
      421 tgattccgct atccttttat ccacatcatc caaccgttct ttgtatttta atgcattttc
      481 attgtatacg tccttgtttg cggggtccag atcagataat tcatcggcga ttctttccac
      541 cataaccttt accctcttag gagatagcca tatatgggga tcccgctcac cgggagcaat
      601 ctctatatcg gggtatgatt ccgacacgtc ctttgccagg tcaattattt ctaaatcccg
      661 attaaggtcc tttgcccttg gaagtatatt ggatacctcg gagggtacac ctattgaaaa
      721 gtaggcggac gcccgggtga aggcttccag ttgtctgggg gtgggggaat aggtttcagg
      781 actgcccccg gggggtatca tggtgattac ctccaccaaa tcccctgcga cctcttctac
      841 aaaggtggcc tggggtacta tggataccgc tacggtaagc ttgttatcgg gcgcgggagc
      901 gggccgatgg catgcgctta aaaagattaa taacaccaat agggttattg ctaacagaga
      961 ttttaacttc atcatatttt cggcaaagat atccccaccc cttaggtgac gaaggaattg
     1021 ccttacctcc tcctttaacg gatgctttag attaacaatc catgaaccat tgtattatgt
     1081 atataattat aatgctcagg gatttaatgt caatatcaaa acccgaagca aatactattg
     1141 ccataaataa acttatagtc tataatagtt atggcatata aatattacta tgtataacga
     1201 gtcggcaaga tgtcccccgc ttctataggc ggcgagttcc catcagtaaa acaggtgatg
     1261 gatataaatc acccgcctcg ttgaaacctg tgtgttgcaa ttgccaatgc aattgcttcc
     1321 gatgttataa taaatccggt tgggataatc ggcataatgc atggaggtgt tgactaatag
     1381 acaatctatc gcctttaggg cgtttttcta ttatatcttt gataatccct attaaggggt
     1441 acggaaggag agagagatgg aggatactta tataacatgt cacagaaagt taatggaatt
     1501 ggcctggagg atcagaggca gatccaggtg taacctgtcg agcttgggat ttgcttggaa
     1561 tcaatattct attatgaaag cattggagcc cgaggaaggc ttgacggtat cggagatcag
     1621 cactagagtg cacagaaaaa tcagcaatgt tacgccggta gtggactttt tagtggacaa
     1681 gggcatagcg gaaaggatct ctgacgaaaa ggaccgcagg gttatacgtg ttcggctgac
     1741 tgcggaaggt attgatataa gacaaggggc catagatcat catcatgact tccttaacag
     1801 tctctatcaa tcgttgactg aagaggagat gggaatcttt ttagatatag tcaacacatt
     1861 catggaaaaa gtcaaatgat cagtctgttt aataatagac gtactaaacg aaagtcgtgt
     1921 tacgtacagg agggatggct atgaataaaa aatcatttct ttttaaatat ctggcaaaac
     1981 ataaattgag atatgttaag ggattgtttt tgctgattat aataaacttt ctccaagtgc
     2041 tggtcccaag gataacaggc caggtagtag acggattgga gagtctgagc ctggatagca
     2101 gaaagctttt atattatggg ggtatcttga ttctaatctc attgatgacc tttattgcac
     2161 actttttatc caggctgcag gtcgccgggg ccagcaacct attcgattac gaggtaagaa
     2221 atgggatgtt tgatcatatg ctgaagctct ctatgaagtt ctttcataga aatcgagtag
     2281 gagagcttat ggccttggct accaatgact taggatccct taggatgata ctatccaggg
     2341 gatttaatct gatgaccaac accgtcattc tattgataag cagcatagtg ataatggtta
     2401 agtatgtaga ccccagactt actctgtatt catttttgcc gctgcctatc ttggtattca
     2461 tcatgacaaa attcggtccc atgataaaca gaaggtttcg cagggtacag gaatccttca
     2521 gcgatgtgac ccgtaagacc acggaaaaca taaccggcat acgggttgtc aaggcctttg
     2581 tccaggagga ttatgaaaag gaaaacttcg caaaggtgaa tcaagaaaac tttgatatca
     2641 atatgaacct agttaaactc caggggatat tctaccccct tatcaacgtc atatcgtccc
     2701 tatccttttt aatcgccttt atatacgggg gtaatctcat tataaccaac cagatcagct
     2761 taggtgattt tatcgccttt aacggctatt tgggaataat cattaggccc gtaatgtcca
     2821 ttggcatgat aatcaatttc acccaaaggg ccaaagcctc tgtagagagg attgaggaat
     2881 tatttgatga gaagccggag gttgtcgatc ccgcggcctt aaatccgggg aaagagcaca
     2941 ttgaaaagga tttttacggc aggatagaat tcaatgattt gacattttca tacgggaaaa
     3001 cggaaccgga tgtccttaaa aacatagacc tggtcattga accgggtaag acccttgggg
     3061 ttatcggaaa ggtcggcagc ggtaagtcca caatagtgaa cctcatatta aggctgtacg
     3121 acccggcaga gaaggggcag cttttaatcg acggggagga tattaccgaa acatccctta
     3181 agaggctcag ggataatatt ggttatatac cccaggacaa ctttctcttt tcctcttcca
     3241 taagggagaa tatcggtttt tcgcccaatg agcctactct agacgagatt cggggggcgg
     3301 cggccatgag ccagatcgac gagagcataa tggaactttc ggatcaatat gagactatgc
     3361 tgggcgagag gggcgtaaac ctttcagggg gacaaaagca gagggtatcc attgcaagag
     3421 cattggttaa ggatcctaaa atcctgatat tagacgattg cctgtccgct gtagataccg
     3481 ataccgagcg caagatatta aatgagctaa aaccctttat ggaggagaga acctgtgtga
     3541 taattgccca caggatatcc agtctccagg atgcggacga gatcatcgta ttggatgacg
     3601 gcaggatcat agagagggga tcccacgagg agctcttgga gcaacagggt tactataaca
     3661 gaatatatca aaggcaactc ttggaagagg agatagagat cgcttaagta agcaaaacga
     3721 agggagatgt tttacggtgg aagataaaaa taatgcccgg gaaagggtat atgacagaag
     3781 actgataaaa agaatgctgg aattcgctat cccctattgg catttctttg tcattgccct
     3841 attattaatt ttaaccatta caggggcatc cctggccagg ccgtacctga ccaaggtggg
     3901 gatagataag tatataaatg gtgtcctcaa aaacaccatg accatagacg aagccagccg
     3961 cgggatatgg gtgttgggta ttttctttct tgtattgatc atagtggaat ttgccctggg
     4021 atacttgcag cggtttatat tggaatatac gggtaagaaa ataatttata atatccgtga
     4081 aaagatcttt aatcatattc aacacctacc cctatccttt tttgataaaa tggctgcggg
     4141 cagaatcgta acaagggtta ccaatgatcc cgagactatc aacgagatgt tctccggggt
     4201 cttagtgggc tttataaaga gtatggtgga gatggccgct attatagtca tcatgtttaa
     4261 acttagctcg aagctgaccc tggtcagctt tacagtgctt cctctgatag tcatcgccac
     4321 ggtgatcttt agaaaaacag ccagaaaggt atggcagagg ataagagcaa aactgtcggc
     4381 tataaatgcg ttcttagctg aacatatagc cgggatgagg attatccagg cttttaatat
     4441 gcagggcaaa aaggccgagg aatttgataa gaccaacaag gaatactacg atgcccatat
     4501 gaagagtgta atcgttatgg ggatattccg accctttatg gacgttgttg aatccttggg
     4561 aatggccctc ttattatggt atggcggcag aaatatactg gcaggggcat tggagtttgg
     4621 taccctttac atgtttgtcg attatatagg aaggttctac tggcccataa gggagcttac
     4681 ggagcagttt aacaccctgc agagttcaac agtctccgca gaaagggtat tcaatctgct
     4741 ggacgagaag caggagcagc agatagaggg aaccatccaa ggttatgata atatggaagg
     4801 cgagatagaa tttaagaacg tatggtttgc ctatatagac gaaaactggg ttttaaagga
     4861 tatatccttt aagatacggc ccggagaatc cgccgcattt gtaggcgcca ccggggcggg
     4921 gaaaaccagc atcataagcc ttttatgcgg attttatgaa caccaaagag gggagattct
     4981 gatagacggg gtggatatca gggacatagg aaaggaaaag ctaagggata atataggctt
     5041 ggtgctccag gatgtatccc tgttctccgg ggatatcgcc accaacataa gactgtttga
     5101 tcccaatgta tccatggagg aggtagagag agcggcaagg cacgtaaacg cccatgaatt
     5161 tatatcaaag ttatacgggg cctacgatca tgagataggc gaggggggca ccaccctatc
     5221 cgtcggacaa cgccagctca tctcctttgc aagagccctc ataagggatc caaagatact
     5281 ggtaatggac gaggctactt cccatgtcga taccgagaca gaggagttaa tccaagatgc
     5341 cctcataaat ctgatgaagg gccgaacgac tatcgccgtt gcccataggc tatccaccat
     5401 acagaatgcg gataagatca tactcatcca taggggcagg ataagggaga tgggcagcca
     5461 tcaacagctc ctcagcaaca gggggctgta ctataatcta tttaggctgc aatacgatcc
     5521 ggaatataag gtaggctagg tttatcttgg actcatagct ttaaagagag ggaataaaaa
     5581 tggtcagact tcggtttatt cataccgcag atgttcatct gggcagcctg ttgcatatgg
     5641 gcggggataa tttgccccct ggcgtgaagg aggctgctat aaccgctaca tcggaaggat
     5701 ttggcagggt ttgccatgcc gccattgaaa atagcgtgga ttttatagtc atttccgggg
     5761 atctctatga tagggaggcc cgctccgtga gggctatgag cttcttcgcg gagcagtgca
     5821 gacttctcga agacgcgggg attcccgtat ttatcatagc gggaaatcat gaccccctaa
     5881 aggaggaacg ggatctaatc agggtgccgg acaatgtgaa ggtactaagg ggtgactgtc
     5941 cggagacaat cgagctttta ggtcctgacg ggaatcccat ggcaagggta ataggtcaat
     6001 cctatcaatc cgggtccctg gggaagggga tacatttaga ttacaaggtg ccggatgggg
     6061 acatttggaa tattgccctt ctccataccc aattggagac gcctcgttca aactacgttc
     6121 catgttccac ggcggaatta aaggaaatac cccacatcca ttactgggcc ttggggcata
     6181 tacataagta tcgaaggctc agcaatgaca tcccctacat tgcctatccg gggatccccc
     6241 agggcaggga ttttggggag acgggcagag gcggatgtat actggctgat ttggatccct
     6301 ttggcgaaag ccatatttcg ttcatgccca tatcaccggt tatatataaa aggatagata
     6361 tccttataga cgatgatctg gagaataccc cagaaaccct tcaggatctt ttagataaga
     6421 tctgtatgga aggggacaag atcctccgcg aaaccggaga ggacgaggat tatcccgtaa
     6481 ggggctatgt ggtggaatgg acaatcaggg gaaggggtgc cattcatagc cttctgaaac
     6541 agcaggaaca ggagtctata gaatttctgt tagattccct cagggaagag tacgggagtt
     6601 ttgatccttt tttatggacg gatgctgtaa ccttaagaac ccaatcgccc atagactata
     6661 ccggtttact ggagaacagc cctgttttca aagaattgga caggataatc ggccaatgcc
     6721 taaaagatga gggaatgaga gaaaggctga tggacgagtt ggggatcata tggaggggca
     6781 gcggggatca cgaagccgaa gatgacctgc gttttcatat ggacgacgcc tccctagagg
     6841 aagtcctcca aagagcaagg tatatgatcg ctgagaaatt ggttgaaagg ggggagcagc
     6901 catgaagatc caaaggctct acatagggga ttttggaata ctcaggaacc agacattgga
     6961 cgaactgggc ccgggcatag tggtgatcgg cggccttaat agggcgggaa agtccacttt
     7021 gatgcatgtc ttgaggcatc ttggctatgg attcccaagg gattccaatt tgcccccggc
     7081 caacagcagg tatgaagccg aggcagatat aaggcttggg gcaggggatg tgtacaacct
     7141 gagactgaaa ggatacggtg agcccatact aaagaggata accgggctcg aaaccgatat
     7201 atcggccccc aaagatctgt acggattgga tagcttttca tataaccggc tatttaccat
     7261 taccttggac gagcttagca taaatcgggg tatatccgac gaggataaaa tgaggcttca
     7321 atccattcta ttgggtgcgg gtttcagcga gatgctcctc ctgccccaac tggaacgaga
     7381 cttctatagg gaaggggata gcataggggg aaaaagaggt aatccgggtg tgaaacagtt
     7441 tcgaccatat tccagtatca ttgaggccgg taggaaacta aagcaaaggg gtctatcaca
     7501 gatcggggaa tatcaggata agcagcagga attacaacat atggacgagc ttgcggaggc
     7561 cttaaacaac gatatcaagg agctggataa tctgatcgtg cagctggata tcatcaaaaa
     7621 tagctatggc agctatctta gggtacagga actaaaaacc tggctggacg tcaacaagga
     7681 tcaagaatgg gaggatggcc ccagggaaca tcaactggaa aggattgagt ccctaaggga
     7741 agaatatatc gggctatgca gaaaacttag ggaaaaggaa ctccaatcgg gattaaccaa
     7801 ccgacttgaa gagagtctgc ttggcgtcaa ggaggagatg gcggacctgt tggccggaat
     7861 ttcgggcgta tatgagagga tgcggggcct tgggcagcga aaacaagagc tggacaggaa
     7921 aagaagagat atcatcctaa aggcgggtga agccaacgcc cattggggcg aaaccgatat
     7981 agattacatt acccgtataa aggcggacaa tgtagagcat aacaggctca atgaattggt
     8041 agaggaatac agagaattgg agtacagaaa gaggacccag gcagatatcc tccaaagggc
     8101 acgggaagag gctcaggcat ttaaagatag aacagcagaa gataatgacg gaagaccatg
     8161 gatcgggata aagaaatatt tttatttttc ccttatactg accctgcttg gcatggcatt
     8221 atccatgatg aatccactgc tggggctaat agtcggtgtt ggcgggacag tgggtgctgc
     8281 tctttttttg attatgaaaa ccttagggcg caggaccgat acgagagcgg cctatgaaca
     8341 ggaaaaggaa ttaagaacga gacaggccaa ggtccgcgcg gaacaaggca tcctagagca
     8401 attagaatcg aatcttaggt tattagagga gacccttaac gattataagg ctaaactggg
     8461 cctgcctgag agggcatccc atagtgcttt gccgggacac ctgttgagga taagggacat
     8521 ccaaaccaag gcggccgagg tggattggct cctgggtgaa ttggacgata ccaaaaagct
     8581 tatagggaag cagtatgtca ggtataaaaa attcttggat caattctgta gcggcgaacc
     8641 ccggagttca gacggcaggg ttgacccttg cgacggggag gagtggaaca ggatatccca
     8701 tacgcttaag ctatgggcgg accgattgaa ggacgcggaa gagctgggcc tcctaaggga
     8761 tgaaaaagca gccataagga gagacattat ggatatggtt ggaaaatatg atccggcaga
     8821 tcccatcccg gaagaaagca acgcccgcag tgattgtagc ggtccctttg acagtcttgt
     8881 tctagatttt atcgaaaggg cccaagatgg catagtctat gcagaaaata aggcatttct
     8941 aagtgaatct attcaatcca tagctggctc gatgtcctca gacggaatac gcagggcatt
     9001 tattccggag tctgcagaca gggcggatct gcttaaagcc tttattgaga gatgccaaga
     9061 atatgcctct gttgaggagg cggatcggga atattcaatg aagatgggtg aacgaggtgc
     9121 caagttggat gagctggatg gggtcaagga ggctatacga aaattaaggg atgatcttga
     9181 gcgtctggga gctgtcgaaa atctaaccct gggtcaaaga cagatagaca gcgggaaggc
     9241 agaactgaag gtattggcgg atcaatatgc cgttaatatg acctcagcct ttctcctaag
     9301 ggaagcggag aaaaatttgc tggagggtat gaaagatagc gttatgaaga gtgcgggtat
     9361 tatctttaat aggatgaccg ggggtcatta tcatggcata ttgccttccg agcccctact
     9421 ggaatctgat tttcaagctg tgccggatgg ggacggcgaa tcacagacaa ttgacatgct
     9481 gagcagggga actcgagaac agctgtatct ttcggtgcgc ctcagccgta taatggacat
     9541 taagccttgc ttgcccataa taatagacga ttccttcgca aactttgaca gcatacacct
     9601 taatcagtcc atagatatcc tatccgagct ggccaaaacc caccagatat ttatactgac
     9661 ctgtcacggc gaattggtgg ggagaatagc cgaatccggg tgttgtgccc agtattggaa
     9721 gctggagcgg ggcaagctga aggtcaccga ttgtgacgag ctgagtgatc atctgtggca
     9781 gtaaaatagg gccgaaactg cggcagtttg aatgacgaag atgctaaatt ctataagggc
     9841 tgcagcagag agctgtgata ttactagcaa aagaaggaga agttatatgg attatctgat
     9901 aagggatata aatcttgcac cggaaggata tggaaggata aattgggtaa agggatatat
     9961 gcccctttta aatgaattgg agagggaatt tgaaagggaa aagcccttta agggtaaaag
    10021 aatagccatt tcggtacact tagaggccaa gaccgcatat ttggctctcg tacttacaaa
    10081 aggtggggct gaagttgccg ttaccggcag caatccccta tcaactcagg acccaatcgc
    10141 cgctgctctg gtaaaggagg ggctgtccgt atacgcctgg cacggggcga ccgacgatga
    10201 atacaaccaa catctcaata ggactttgga ttttgggccc catataataa tagatgacgg
    10261 cggggatctg gtacaccttc tccataccac cagggcagat ttggccggcg gagtgctggg
    10321 gggctgtgaa gaaaccacca ccggagtgct gaggcttcgg gccagggaac gggagggcat
    10381 attggacttt cccatgattg cggtaaatga tgcctactgc aaatatcttt ttgacaatcg
    10441 atacggtacc ggacaatccg tatgggacgg tataatgaga actaccaatc tgatggtttc
    10501 cggcaaaaat gtggtggtag caggctacgg ttggtgcggc aagggagtcg ccaataaggc
    10561 caagggtttg ggtgccaatg taatcgttac ggaaatcgac cccataaagg ctatagaagc
    10621 ggtcatggac ggattccggg ttatgaccat ggatgaggct gcccccatag gcgatatatt
    10681 cgttactgta acgggatgca acaaggtcat aagggaagag cattttataa agatgaaaga
    10741 cggtgtgctc ttagccaacg ccgggcactt tgatgttgaa gtctataaac ccgatctgga
    10801 aaaattggcc gtcgaaaaga aggatatgcg gcagaatatt ttaggctatg ttatggaaga
    10861 cggcagggta ttaaatcttc tggccgaagg caggctggta aacttagctt cgggggacgg
    10921 acatccggcg gagatcatgg atatgagctt tgccctccaa gccttatcgg caaagcatat
    10981 attagagcag cattcggtta tgaataacag ggtatacaag gtgcctgaga agatagataa
    11041 cagggttgcc gccatgagac tttcggctat gggcattaat atcgacatcc ttaccgacga
    11101 acaggaggcc tacttaaagg gttggggcag caccgcctcc tactagtagg tggtgctcgc
    11161 cccagccacc aatatctagc aggcattgac ggcgtaacta taggcaaaca ataggtcaat
    11221 taaatttatc ggcatgataa ttgtagcagt tctgagcaat tatcatgccg ataaattttg
    11281 cagttggagt tgtttgtcta taatacactg cgccggttag gcgtatggat ttaagtcacc
    11341 cgccttttaa ttaattattc attcaaggtt caactttctg ttcagtatga tattggccga
    11401 tgcaaagttt gctgcgccca gcagcgaata cagaaccatc atggaaatca taagggttaa
    11461 tatttgggca tggatgggaa ggtccgattg ggccaggggc tgaaagatag cgttggccgt
    11521 tgcaaatatg aatatggtca ggactatctg ggtgaatata tagataccta tatacgcccc
    11581 aaaggaagcc aatatcctat gctttgtaaa taggtgccct atggaaaggg cattgtagac
    11641 ggtcaggata aagtatgttg atcctattag agaagaaata ggtgccagga tcaggaacac
    11701 atccccaaag aacctacgcc cttccccgat tatcctcact atctcgccga acagattgtc
    11761 tacccttata aggaccagca cagaagagac gataacgata aagctaagta tggtccatag
    11821 cagggagacc aggagtttgc ttatgatatg ttgccaagtt tttaccggaa gcgtaaacat
    11881 aaggtaccct tcatcgccta atagattctt ataaaatctt tggataatga taacaaaggt
    11941 catagcaatg gtagccacga taagggcgaa atagataaca acgcttgcaa ttcgcatcat
    12001 gttgaggaag ctaaagccct ctattgtaac gcctgtggta tctccaacct gctcaaaggg
    12061 gtttattacc ctatttataa aagcgaacag cagtattgca atgtatacgg gtaaaaatga
    12121 acgtgcagta gccttggttt catattttaa aagttttcct agcatttgaa tacctcccta
    12181 aacaaagcat ctacggattt accggactcc tcgcgtattt catccacagt tttgttcatg
    12241 accacctggc cctctttgat aaagatgatg tcatctaata tcttttctat gtcggatatg
    12301 aggtgggtgg aaatgatgac cgtcgcatct tcgctgtaat tgctgaggat ggtgtttaga
    12361 atataatccc ttgcagcggg atcaacaccg cctatgggtt catccagaag atagagttta
    12421 gcttccctac tcataaccag gatcagctgc accttttcct tagtgccttt agacatggtc
    12481 ttcagtctgt cctttgggtt tatgtttagc ttgcccagca tatcataggc ctttgccggg
    12541 ttgaagtctt cataaaaatc tttaaagaat tctatgatat ccgatgccct catccaatcg
    12601 tttaaatagg tcttctcggg gaggtaggat actacacctt ttgtccttat tccaggtctg
    12661 tctccgccta taaggatctc gcctttggtg ggggtcaaaa gatcgttaca cagctttata
    12721 aaggtggttt ttccgctggc gttagggcct aaaagcccaa taatccttcc atggccgatt
    12781 tccaggttca catcccataa tgcgatagtc gacccaaagg tctttgtgag atttttgcac
    12841 tctaaaagat tactcatttt ttcaactcct tcgatatatc acccaacatg gatataatgt
    12901 cgtcttgatc gaatcccagc ctgatcatct tttccaggaa ttcttggaca tgttcccggg
    12961 ctaatcgctt ctttgtcgtt tttaccatat tgatatcctc cgttacgaat ctgccgctgg
    13021 tgcgaaggct gtatagtagc cccgtttctt caagtcttga cagggctctt tgcatggtgt
    13081 tcggatttac ggatgcttcc tgagccagat ctctgactga agggagcttt gaacctagag
    13141 gatagacccc ggaacaaatc ttcagctcca tttgctcaat gatctgggca tagattgggc
    13201 gatccgatgt tagatcccaa tgcataagat cacttccttg tactattgta ttaatggcct
    13261 aatacaatag tacataacct ttccggtatt gtcaatagct tttaaaagaa acttggataa
    13321 tttaacccgt tgtgctagtt caccagccca ttgtgtagta aaggtaatga actattgaaa
    13381 agcggatgac ttcattgatt tggctaagaa aacttggcct atggagtgca gaaaagccgg
    13441 agtttaaggc aggacgcctt aaaccgattc caccggcagg aggccggatt ggaagcgata
    13501 ggcttttcaa atggaatagg ccttagtttt ttccaaatca atgtcggaag gagcgttcaa
    13561 taatcattac tgatatgttt agctagcaca acgcattaat tcaaaggatt tattttgtgc
    13621 gttaaaaaac ccgataccgg acagttttgc acgcctggca tcgggtttta aattcgatgg
    13681 ttcaatattc ttctctgagg ccgaattcgg ctgactcaac ccattctacg gctcgattca
    13741 catgggacca gatttcctcc agcaggttat aatggatttg ctcttcatgg attaaaaatc
    13801 taaatacttc cctgtcgcca tcatcccttg cctcctccag catccccttg tacaggttaa
    13861 tactctcctt ttccttatcc atcgccacgg aatagacatc cagttggtcc gggatatcct
    13921 ttatgacatt atgaaaatcg ggagtatctt ggaacacatt cttataatcc gcgtaggatg
    13981 tggtttccga taggacggtg tccagttgtt tcatcctatc cttcaggatt tgggcatgtt
    14041 ttctttcgtc ccttgcaagg gttaaaaata tggtatgcag tccgctgtcc ctatgcttct
    14101 ctgcctgttc catgtaatac ctctcgccat cgagttccat ctgaatcgca aattcatatg
    14161 agcgcatgat atcatccctt caataatatg tattagacaa tgtataccac tgatgcaagc
    14221 ccgataacca tatattttat gcacatgcca tggaagggat tgccaccctt ttcaatagca
    14281 cagccgatta aagataaaca ttacgaagac taaatttatt ggcatgatag ttgcaacggt
    14341 tctatgcaat ttcatagtgt gagttgctta tctggagata aacattacga agactaaatt
    14401 tattggcatg atagttgcaa tggttctatg caatttcatg gtgataaatt tcatagtgtg
    14461 agttgcttat ctagagataa attttatagt cagagttgtt tatttataaa gtgtagtata
    14521 atggaagcag atatttatac agggattgtc aatagaagga aagtggaacc aaaagcatac
    14581 aagaaggtgg catagttgaa taggtatttt aagtttttta tcttaatggg cataattttc
    14641 ggggcgtttt tcttgattcg cgaatatatt ccaaaggccg atgggattag gcagaccccc
    14701 aacggcgggc accgcttctc cgaacagatc gaagagaacg aagggctgtc tcccgcaaat
    14761 gatgaaaagg attctccggt gagtggggac gataataatg atgtggagga taaccctcag
    14821 ataacctcag tccctaataa cggctatata aatatctcag ggatgactat aaaagagaga
    14881 tatagcctgc cccaggggta ctctagggtt tcggcggagg aggggagctt cggcgagttt
    14941 ttaagaaacc aaaggctaaa accatatggt gaaaagtccc tgtattatga cggcagagaa
    15001 aagccctccg caggaatata cgatggagtc atggatgtgg atattgggaa cagagattta
    15061 catcaatgcg ccgatgctat aatgctcttg aggggggaat atctttattc tctgggtgct
    15121 tatgaggata taaactttaa ttttgtatct ggctttaggg cggagtacaa aaagtggacg
    15181 gacggctacc ggattaaggt caacggaaac aaagtagaat attacagggg tgctgatccg
    15241 tcaaataccc acgaggattt cagaaagttc atggatatgg ttatggccta tgccagcacc
    15301 ttatcgttgg aaaaggaatt ggaatccgtg catatccagg atttggatat aggcgatgtg
    15361 ttcattatcg gtggtagtcc cggccatgca gtgatagtgg tggatgtagc cgagagcgca
    15421 gataatgaga aaatgtttct cttggcccaa tcttatatgc cggcgcaaca aacccaaatc
    15481 attataaatc ccatggatag ggacatcagc ccatggtata gtttggatgg aaaggatgaa
    15541 ctcattaccc cggaatggag atttagtctg gataatttaa aggggtttcg ttctaatgat
    15601 taaattaatt tgaatattat aaggagctgt gataatttca tgaaaagaac atatcgttgg
    15661 ttatcaatct tgcagtggtt tgttggggtg ggggctatag gaggcggaac tatggccata
    15721 ctctttcccg aggggcctgg gggaatgccc gccgatgcct taaaatacgc accttttgat
    15781 aattatctca tacccggcat tattctgttt tccgttatag ggctgggaag tatattgagc
    15841 gcattctcaa tacagaagaa aatgaaacac catgcttaca tagccatgac atctggctgg
    15901 gccctggtta tctggatagt agtacagtgt ataatgcttc ggacggttgt agccttacat
    15961 atcatttttt tcgtcatcgg cttgattgaa gtatttttat cgggcatcct atttatcaga
    16021 cagaacttat ttcccatcaa tctcatcaga ggcattatgt catcatccaa gaaaggttct
    16081 gcagagttga aattgcctta gctacgggat gaactaccat atttaattcg ctaaagagtc
    16141 gaagccatat agtgatgtgg tataatgtgg atatggatgc aaggcggtgg gtataaatca
    16201 cccgcctcgt tgaaacctgt gtgatgcaat tgtcaatgca attgcttctg atgttataat
    16261 ataggctgtt atttagggta caaaaggacc tataaaatta taaatatgcg aagggaagat
    16321 aagaatgaaa gtgatagttt ttttagcgga cggttttgaa gaggtggagg ctctgacagt
    16381 ggtggattat ctcaggaggg tggatatatc cgttgaaacg atatccatta cccgtgagag
    16441 ggaggttatg ggcgcccatg atattccggt actggctgat aagacgatag acgaggtagg
    16501 ggatgcagaa ttatatgacg gggtaattat cccggggggc ctaccggggg ctacgaatct
    16561 cagggataat ccaatggtca ttgaaactgt aaagggtgta tatgagaacg gcaaattagc
    16621 tgccgcaatc tgtgccggtc ccatagtgct agagagggcc ggaatcatag aaggcagaag
    16681 ggttacatcc tacccgggat ttaaagatca attaccgggc agcatctatg tggaacaggg
    16741 cgtagttagg gacggaaata taattactgc cagaggccct gcgttggcgg tatattttgc
    16801 catagaaata gcagaatact tcctaggtga gaagaaggct gaggaactac aaagggccat
    16861 attgcttcct cagacctaga tggtaagaca tcaggttgga tatcccttat cccaatccta
    16921 aaaatccaga tttcaaatgg tcagccccaa gatgcccggc gcaaaaccga gtatcttggg
    16981 gttgactaga ttattctgta ccctctatgc tatgaaattt atcggactgc acgcttagaa
    17041 tcacttctgt ttttgttctc tatgaatatg ataatgagag aaagggatat agacctataa
    17101 cgcagcaaag gacattaggg gcagagagac ccataaatat cgatgtattt ttgggagttt
    17161 gtgtgataat ccgtcgatat aggtaaaaag cataggaaat cggaaattag ggcattgaca
    17221 acaggtacaa attggtttaa aatgaaataa caacatttat agtaagctaa tgacatttat
    17281 ctataaaaca tttttgcggg ttgctatgtg ttttatggca aacatcctac attagtat
//
LOCUS       DASRNX010000257         7866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_740899,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000257 DASRNX010000000
VERSION     DASRNX010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740899"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..516
                     /locus_tag="VFD89_10285"
     CDS             250..516
                     /locus_tag="VFD89_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58605.1"
                     /translation="MKAIDCRKKASNIDENGDYAGDLLEEIRGGDNKIFRAPKPDCTT
                     RNRTNNTGNRTRQGLGPEGRDAPYADFGQRSIMSKGTRISIPLK"
     gene            complement(500..1102)
                     /locus_tag="VFD89_10290"
     CDS             complement(500..1102)
                     /locus_tag="VFD89_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZJ58606.1"
                     /translation="MRVFEYGQKEIDYLKKMDKVLGSAIDEIGIIKRPIMPDLFTALI
                     RNIVGQQISSKAANTVWNRMLERYSPITPDIIMAATVEDIQKCGMSTRKANYIKGIAD
                     AVACGELDLSELEHLSDDEVIKRLSSLPGVGVWTAEMLMIFSIQRPDVVSWGDLAIRR
                     GMMTLYGLDDLTREQFYKYRERYSPHGSVASLYLWVISKE"
     gene            complement(1410..2684)
                     /locus_tag="VFD89_10295"
     CDS             complement(1410..2684)
                     /locus_tag="VFD89_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HZJ58607.1"
                     /translation="MNKPLTLIIMTLAITFLLTGCYDAHEIDDITHVLAIGIDRGVSD
                     KWRLSLQFSTMKGESGGSEEEGNGGGSQGDYTYMTVDAPSFFAGVDMLNSSIPRELFF
                     SHTELVAVSEDLAREGLMGEFISPLTRYTQIRGSTRLMVIKGSAQDFIKENEPYIGTT
                     LSKAMETSAKESMNIGYFGHTTLSDFYNDLKSTYRQPIISIGAVNEFKNFKEDGPKWD
                     GGFEDEGKYLAGELPRYGQKKIEYWGTALFNGKKMIAELNGSDTRALLMVRGDFQRGF
                     FTMQDPKVPDLIIPLDIRPDGAPEVKMGFQDDVPNIDLTVPLKGDILAIQSTLHYEED
                     PLLSLLERTFERKIKDEIDKLINRCKGMGIDVFQFGDISARYFATIPEWEEYNWNNKF
                     KDAKVTTQVKLVIKRAGTRTKSSPIPNRREGE"
     gene            complement(2677..3774)
                     /locus_tag="VFD89_10300"
     CDS             complement(2677..3774)
                     /locus_tag="VFD89_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HZJ58608.1"
                     /translation="MIKEGKFGVQEAVWLVVFAISTKVFYTSPARVVDLVGNTGWYMT
                     LISMSVAILGFTFIYLLLKRFPGQDIASIFADTLGGPLGFVFSAILALYLLLLATNTI
                     SEFAEVIKVYVFPLTPSSLIIGIFVVGVLIMCILGLETQARFAKLSAYTILFGFILIL
                     ALGIENYDVNRLFPILGYGADKVLRHGLIRSSAYGEIIILAVIAPSLQGTAFVKKAGY
                     TGLILSGFFISISALAFALSFPYYTAQEITAPMYEMAELINYGRFIQRVEPIFLFIWF
                     FSSFTSISILFYCFISTYCRIFKIRDIRPIALGAAIILFTSSLFQIDLGVGAAQRIQN
                     IRQYGWIPPFILPFIALVVGVLRKRGVKSHE"
     gene            complement(3771..5534)
                     /locus_tag="VFD89_10305"
     CDS             complement(3771..5534)
                     /locus_tag="VFD89_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HZJ58609.1"
                     /translation="MGNQFELLEDASEKGDPAQISKDKGNPPQTPQSEMGKNNGKDDT
                     HNSPESKESRGKKVPLRVDEWNMERKQDSAAPDIANSREGGSISDNLEQNRLRIMREL
                     NIPTNVDAMIRDLRIGKSTKAFIAYIEGMVDRTTINDFILRPLMTVKHFNSPLEECSA
                     NYILENILSIDDVTKASDYTQIISQILEGLTALFIEGSDSCLLIESRGFEKRSVDQPV
                     TESVISGPHEAFIEDLQTNLSLVRKIIKNKNLITEILPIGKTNNSRCGIMYIGGIVNT
                     SIVDEVKRRIQSIDIDFILGDGMLSQLIEDHPLALFPQILRTERPDRTASFLVEGKVI
                     IICDGTPHASIVPVTFFHMMQTSEDSFLRWQFGTFLRLIRFMGMGLAMLLPGFYLALT
                     LFHHEMIPTELILAIEKSRENVPFPAILEILLMEFSFELIREAGIRVPDVIGQTLGII
                     GALILGQSAVAANLVSPILVIIVAITGLGSFSMPNHDLAIAIRIIKFVFIALGAAIGF
                     YGIAMGIFVIGGFACNMKSFGVPYFSPVAPRTRINLDVIFKGPLWKQTLRPDPSNTLN
                     RTRSGDVVREWIDTGEGGEQE"
     gene            6059..6916
                     /locus_tag="VFD89_10310"
     CDS             6059..6916
                     /locus_tag="VFD89_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZJ58610.1"
                     /translation="MERSVEAVYSTQLMKKMKAGFIGFMPYGDPNVDLYDLLESYGEI
                     GYRGFEAGAMLLRGNPIENLKRVQGFGMQPLTLGYRIGGDNSVDELIKNAKMLGIDRV
                     TTFVGVAGAYRFKDILEAPTYDDVMREIEEIEGVAKRLAEEGIIFAFHNHDVEFDMAF
                     KGVPLLYLMLAHSEHLKIELDVGWVKYAGRDPAVVMKDIGNRICALHIKDITDGTVID
                     EKPNGPVTMPQFTTPGTGLLNMKDCLSVGVELGIEWAIVEQDFQYNISQKETLTAAYL
                     NMKETGLVE"
     gene            7105..>7866
                     /locus_tag="VFD89_10315"
     CDS             7105..>7866
                     /locus_tag="VFD89_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HZJ58611.1"
                     /translation="MPKNFHLAVDLGTSGCKITVFDFNGLPVASRTGGYKTYYPGPGS
                     VEQDPGEWWKVVCQGISAVIDECSIDPKEIAAIGVDGTSWACIPVDKSGQVLRPAMLW
                     LDRRAQPQAEWMKGRVGEDRLVETSGNPVDAAYITPKILWLKDNEPETYRKTDKFLQS
                     NGYIVYKLTGRYSQDYSQGYGLHFFNIRKGCWDEGIAHELGIDLDLMAPLYHSHEVVG
                     TVTVQAAKETGLTPGIPVVAGGLDAACCTLGAGVIM"
BASE COUNT         2143 a         1725 c         1887 g         2111 t
ORIGIN      
        1 ttatacaaca gggcgaatat aaatgcaagg gccaatttgc agttctgaat tttgggtttc
       61 cgtattaatt gattcgcctt atccatcctc tatttttagg ggacagcggg aatatgaaac
      121 aatttttagg ggcagcggga aaacaaaaca atcaaaatag ggtttgaggg aactgatatg
      181 aatcataagg aaaataaagg agggatatca taatctaaaa acatgccact acacaggtga
      241 tcatatagaa tgaaggccat agactgtcga aaaaaggctt caaatataga tgagaacggt
      301 gattatgccg gggatcttct ggaggaaata aggggagggg ataataagat ttttagggcc
      361 ccaaagccag attgtacgac cagaaacaga accaataaca ccgggaacag aacccggcaa
      421 ggtcttggcc cggaaggccg ggatgctccc tatgctgact ttggtcagag gagtataatg
      481 agtaaaggaa cgcggatatc tattcctttg aaatgaccca aaggtataga gaggctacgg
      541 acccgtgggg cgaatagcgt tctctgtact tatagaattg ttccctggtc agatcatcaa
      601 ggccatatag ggtcatcata ccccttctga tggcaaggtc accccaactg accacatccg
      661 ggcgctgtat ggagaagatc atgagcatct cggctgtcca gactccgact cccggaaggg
      721 aagacaggcg ctttattacc tcgtcgtcgg aaagatgctc cagctctgaa agatccaact
      781 ccccgcacgc cacggcatcg gcaatccctt taatataatt tgcttttcgg gtagacatgc
      841 cgcacttttg tatatcctca acggtagcgg ccatgatgat atcaggtgtt ataggtgaat
      901 atcgttccag catcctattc cataccgtgt tggcagcctt ggaggagatt tgctgcccta
      961 ctatgtttct tataagcgca gtaaatagat ccggcataat aggacgctta attataccga
     1021 tttcgtctat tgcactgccc agtactttgt ccattttctt tagataatca atttcttttt
     1081 gaccatattc aaatacacgc acgattttag ccccccaagg tatagtttac gaatagtata
     1141 ccatggttcc cctaagctac aaagcattcc ttctgctggg gtatgtacag tttactccta
     1201 taatcccatg aagaattcgt cgctcggggg attatgcaat aatggatatt ccatcattgt
     1261 gtaagtaaaa agggcatggc gagggtggtt gcggcaatga ttattgcgcc tgtcgctgcc
     1321 gccctgtttt tgttattcca attatatttc gcgaagctca aaagatataa aaatatccaa
     1381 aatgcaagaa aagaaatcaa gtatttcaac tattctccct cccgacggtt cggtatcggc
     1441 gagctcttag tcctggtacc cgccctcttt ataaccagtt ttacctgcgt agtcaccttt
     1501 gcatccttaa acttattgtt ccaattatac tcctcccatt cagggatggt ggcaaagtat
     1561 ctggccgata tatctccgaa ttgaaagaca tctatgccca tacctttgca tctgtttatg
     1621 agcttgtcta tctcatcctt tatttttctt tcaaaggtcc tctcaagcaa tgataaaagg
     1681 ggatcctctt cataatggag cgtactttga atagcaagga tgtccccttt tagggggacg
     1741 gtcaaatcta tgttggggac atcgtcctga aagcccatct tcacctcggg tgcgccgtca
     1801 ggtcgtatat ccaacggaat aataaggtcg ggcacttttg gatcctgcat ggtaaagaaa
     1861 cccctttgaa agtcccctct aaccataagt agtgctcggg tgtcagagcc gtttaactcg
     1921 gctatcatct tcttaccgtt aaataaagcc gtcccccaat attctatttt cttttgtcca
     1981 taccttggca gctcccctgc caagtacttt ccttcatcct caaatccgcc gtcccacttc
     2041 ggtccgtcct ctttaaaatt cttaaattcg tttaccgcac ctatggatat tatgggctga
     2101 cggtatgtcg attttagatc gttgtagaag tcactcaggg tggtatgccc aaaatagccg
     2161 atgttcatgg actccttggc ggatgtctcc atagctttgg ataaggtcgt gccgatatag
     2221 ggttcattct cctttataaa atcctgggcg ctccccttta tgaccataag gcgtgtagat
     2281 ccccttattt gtgtatagcg ggttagtggg gatataaatt cgcccataag accctccctg
     2341 gccaaatcct cggataccgc tacaagctct gtatggctaa agaacagttc cctggggatt
     2401 gaggagttta gcatgtctac ccccgcaaag aaggaggggg catcaacggt catataggta
     2461 taatcccctt ggctgccgcc cccattacct tcttcttcgc tcccgccgga ttctcctttc
     2521 atagtcgaaa attgaagtga cagcctccat ttatcggata cacccctatc gatacctata
     2581 gccaggacat gggttatatc gtcgatctcg tgggcatcat aacaaccggt taacaagaaa
     2641 gtgatggcta gggtcatgat aatcaaggta aggggtttat tcatgggatt taacacccct
     2701 tttgcgcaga acccccacta caagggctat aaacggtagg ataaaaggag gaatccaacc
     2761 gtattgtctg atgttttgta ttctttgagc cgcacccaca cctaaatcga tctggaataa
     2821 ggagctggtg aagaggatta tagcagcccc cagcgctatg ggccttatat cccggatctt
     2881 aaagattctg caatatgtgc tgataaagca atagaagaga atggatatcg aggtgaagga
     2941 gctaaagaac catatgaaaa ggaatatggg ctctaccctc tggatgaatc gaccatagtt
     3001 gattagctcg gccatctcgt acataggagc ggttatctct tgggcggtat agtatggaaa
     3061 ggatagagca aaggcaaggg ctgatatgga gataaagaat ccggacagga ttaggcccgt
     3121 atatcccgcc ttttttacaa aggctgtacc ctgaagggaa ggcgcaataa cggctaagat
     3181 gattatctcg ccataggcag agcttcgtat aaggccatgc cttaggacct tgtctgcacc
     3241 gtagccaaga ataggaaaca gacggttgac gtcgtaattc tctatcccca gggccagaat
     3301 caaaataaaa ccaaagagta tcgtataggc agacagcttg gcaaacctcg cctgggtttc
     3361 cagccccaat atacacatta tcaatacccc cactacaaat atccctatta ttaaggaaga
     3421 tggggtgagg ggaaatacat agaccttgat gacctctgcg aattcactta ttgtattagt
     3481 tgctaaaagt aacagataaa gcgccagaat agccgaaaaa acaaacccaa ggggtcctcc
     3541 cagggtatct gcgaatatag atgcgatatc ctgcccggga aatctcttta gaaggagata
     3601 tataaaagta aatcctaaga tagcgactga catggaaatc agggtcatat accagcctgt
     3661 attacccacc aaatccacca cccgggccgg gctggtataa aaaaccttag tggatatagc
     3721 gaataccaca agccataccg cctcttgaac cccaaattta ccttccttta tcattcctgt
     3781 tctcctccct cccctgtgtc gatccattcg cgtactacat cgcctgatct ggtcctatta
     3841 agggtattgg acgggtccgg tcttagggtc tgcttccaaa gaggtccctt gaatatgacg
     3901 tccaggttta tcctggttct gggggcaacg ggggagaagt aggggacccc gaaggacttc
     3961 atattgcagg caaatccccc gataacaaag atacccatag ctatgccata gaagccgatt
     4021 gcggccccta gggctataaa gacaaacttg ataatcctga tggcaatggc cagatcatgg
     4081 ttgggcatgg agaaactccc cagtcccgtt atggctacta taatgaccag gattggactg
     4141 accagattag ctgcaacggc agattgaccc aatatgaggg cgccaataat accaagggtc
     4201 tgacctatta cgtccggtac ccgtatccct gcctcccgta taagctcaaa ggaaaattcc
     4261 atcagcaata tttcgagaat tgccgggaaa ggaacgtttt cccgagactt ctcaattgcc
     4321 aatatgagct cggtgggtat catctcatgg tgaaataatg ttaaggctaa ataaaagccc
     4381 ggcaggagca tggcaagtcc cattcccata aacctgataa gcctgaggaa ggtgccgaat
     4441 tgccagcgga gaaaggaatc ctcagaggtt tgcatcatat gaaaaaaggt aacaggaacg
     4501 atgctggcgt ggggtgtacc gtcgcagatg atgataacct tgccttccac gagaaaggag
     4561 gctgttctat cagggcgttc tgtccttaat atctgtggaa agagggccag gggatgatcc
     4621 tcaataagct ggctcagcat accgtcccct aagataaagt ctatatcaat gctttggatc
     4681 ctgcgcttca cctcgtcgac tatgcttgtg ttcactatac cccctatata cattattccg
     4741 catctggaat tgttggtttt cccgatggga aggatttctg tgataagatt cttattttta
     4801 atgatcttgc gtaccaggct tagattggtt tggaggtcct ctataaaggc ttcgtggggt
     4861 ccgcttataa ccgattcggt gacgggttgg tccacgcttc ttttttcaaa gccccggctt
     4921 tcgatgagaa ggcaggaatc cgaaccttcg atgaacaacg cagtcagtcc ctccagtatc
     4981 tgggatatga tttgggtgta gtcagaggcc ttggtcacat cgtctataga tagtatattc
     5041 tccagtatat agttcgcaga gcactcctct aggggagaat taaaatgttt caccgtcatt
     5101 agcggccgga ggataaaatc attgatggtc gtcctatcca ccatgccttc aatgtatgcg
     5161 atgaatgcct tggtggattt tcctatacgc aaatctcgga tcatggcatc cacattagtg
     5221 ggtatgttaa gttccctcat tattcgtaac cggttttgct ccaggttatc ggatatgcta
     5281 cccccttctc ttgaattggc gatgtcagga gcagcgctgt cttgcttgcg ctccatattc
     5341 cattcgtcca ccctgagtgg aacctttttg cccctggact ccttagattc cggggagtta
     5401 tgggtgtcat ctttcccatt atttttgccc atttcggatt gtggggtttg gggaggatta
     5461 cctttatctt tcgagatttg ggcaggatcg cccttttctg atgcatcttc aaggagttca
     5521 aattggttcc ccataggagg ccctttatat gtaacgagat ttttgattaa actaaataag
     5581 cccattttaa ccgcctaact atatgtttac gataagcacc ttacattatg tttcggatat
     5641 cgataaacat tttccttgat ttatatgttg cgaaaactac attgatctta ttgatagttt
     5701 ttatcgcgac atatattatc gtgttaacaa aatcctttta aattattatt tcccaaatgg
     5761 ggcttattaa ataccccgga aaatcggcga ccggggttcc catcaatttg taagttggtg
     5821 gtgggcatta aaactcatgg gaattcggcg gttggggttc ccatcaatgt atgggttggt
     5881 gacgaggttt taaaacctat gaaaaacctg cgttgacacg gtataatatt ggaggtatga
     5941 tttaggtata aagtgccaaa aacatggctt tcatatgggt cggagtgtaa atgttttgaa
     6001 ataaccgggc atatgataat gtagagggca atggacaacc cctctgcagt gaaaatattt
     6061 ggaaaggagt gtcgaggctg tctactcgac gcagcttatg aaaaagatga aggcaggatt
     6121 cataggtttt atgccctatg gggatcccaa tgtggaccta tatgatttgt tggagtccta
     6181 tggggagatt gggtatcgcg gatttgaagc tggggctatg ctcttaaggg gtaatcccat
     6241 tgagaacctt aagagggtcc agggctttgg aatgcaaccc cttaccctcg gatataggat
     6301 cggcggggat aactctgttg acgaactaat taaaaatgcc aagatgcttg gcatcgaccg
     6361 ggtcactacc tttgtcggtg tggcgggggc ataccggttc aaagatatac tcgaagcgcc
     6421 tacctatgat gatgtaatgc gcgagattga agaaatcgaa ggcgtagcaa aaagactggc
     6481 cgaagaggga atcatctttg catttcataa ccacgatgtt gaattcgata tggcctttaa
     6541 gggtgtaccc cttttgtact taatgctggc acattcggag cacctaaaga ttgaactgga
     6601 tgttggctgg gtgaaatatg caggaaggga tccggctgta gttatgaagg atataggcaa
     6661 tcgcatatgc gccctacata tcaaggatat aacagacgga actgttatag acgaaaagcc
     6721 aaacgggccg gttacaatgc cccaattcac aactcccggg acgggcttgt tgaatatgaa
     6781 ggattgcctg tcggtaggcg tagagctggg catagaatgg gcaattgtag aacaggactt
     6841 ccagtataat atttcccaaa aggaaacact caccgcggca tatctgaata tgaaggaaac
     6901 cggtttggtt gagtagggaa aagaagggtt acagttcccg ctaaaagtcc aattctatcg
     6961 tgatatttga atggataaac taaaaggtga ttgttatgat tgttgtttgg atctgacagt
     7021 caccttttta aagattggaa attcatatta aaagggtcgg gaatctacga attacaccct
     7081 aatataccta cagggaggat aaggatgccg aagaattttc acctagcggt ggacctcggt
     7141 acatcgggat gcaagataac ggtttttgat tttaatggtt tacctgtagc cagccgaaca
     7201 ggcggttaca aaacctatta tcccggcccc ggatcggtag agcaggaccc tggggagtgg
     7261 tggaaggttg tatgtcaagg gataagcgca gttattgatg aatgcagcat agatccaaag
     7321 gaaatagccg ccatcggagt ggacggtacc agttgggcat gcatccctgt agacaaatcc
     7381 ggccaggttt taaggcccgc catgctatgg ctggatcgca gggcacaacc ccaggcagag
     7441 tggatgaagg ggcgggtcgg ggaggatcgc cttgttgaaa ccagtgggaa tccggtggat
     7501 gccgcttata taacgcccaa aatcctttgg cttaaggaca atgagcccga aacatacagg
     7561 aagacggata agttccttca aagcaacggc tatatagtgt ataagcttac gggtcggtat
     7621 tctcaggatt attcccaggg ttatggactt catttcttta atatccgtaa agggtgctgg
     7681 gatgaaggca tagcccatga actgggaatt gaccttgact taatggcgcc cttatatcat
     7741 agccacgagg ttgtgggaac tgttacggta caagccgcga aggagaccgg cctaaccccc
     7801 gggatacccg tagtggcagg gggacttgat gccgcctgct gtaccctcgg ggcaggggtt
     7861 attatg
//
LOCUS       DASRNX010000258         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Clostridia bacterium isolate SMAG_U10253 k141_741364,
            whole genome shotgun sequence.
ACCESSION   DASRNX010000258 DASRNX010000000
VERSION     DASRNX010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963779
            Sequence Read Archive: SRR5678808
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Clostridia bacterium (soil metagenome)
  ORGANISM  Clostridia bacterium
            Bacteria; Bacillota; Clostridia.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 22:53:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,020
            Genes (coding)                    :: 2,005
            CDSs (with protein)               :: 2,005
            Genes (RNA)                       :: 46
            rRNAs                             :: 2, 1 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Clostridia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741364"
                     /isolate="SMAG_U10253"
                     /isolation_source="soil"
                     /db_xref="taxon:2044939"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..359
                     /locus_tag="VFD89_10320"
     CDS             66..359
                     /locus_tag="VFD89_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZJ58612.1"
                     /translation="MDDKMFALIEKMYVEMQTMKSNKETKEDMSGITQRLTKIDQRLA
                     VMENKMDTNHKALYDGYKLTYEKAVTLEEKIDVIDKKVDKHDVEIRVIKGIKG"
     gene            734..2023
                     /locus_tag="VFD89_10325"
     CDS             734..2023
                     /locus_tag="VFD89_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZJ58613.1"
                     /translation="MKITDVKAAKIGANIVIRVVTDKDGMDGYSQIESHKDYVMPLIP
                     YFKSMIVGHDPTNVERVLRQMRRSAAFKPWGKVFSSIEMALWDIAGKDAGVPVYKLLG
                     GKVRDRVRVYNGSFLDPNPPHEIADTPEKLGENILHLNEIPEGFTIVKVAAFWHMPNF
                     YGESDAYNTYYPQGRGEANRLGGYTNYVKMINEGKTLSSRFNIPVVQGSLLTEKGLKT
                     SIEYVERLKKVVGDKVGLAFDIGPGFMPGDAIRLAKALEPYNVMWLEDSIAGDYTPYT
                     MASVYRDITTSTSTPIHTGEQIFLRQNFMELIETRAVDVVGPDPADVGGIKELKWIAE
                     YADLHGILMAPHGTFNGVFGMAALTHVCSTMPQNYVAYEYPGPNPGFWLDIVEGLPNN
                     FLRDGHVKVWDAPGIGITFNVNKASKYLSPEDKDFFL"
     gene            2479..>2915
                     /locus_tag="VFD89_10330"
     CDS             2479..>2915
                     /locus_tag="VFD89_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014064006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HZJ58614.1"
                     /translation="MLEEKIKLETRLVGDIEGKFYVPSYQRGYRWGKNEVTRLLEDIY
                     SNGENNYCLQPIVVRAKGEHYELIDGQQRLTTIYLVYNYMFRISGGFLGQPKFTLVYQ
                     TRAETEKFLEDIDLSRKEENIDFWFISNAYETIESWFKDKERVS"
BASE COUNT          923 a          574 c          702 g          716 t
ORIGIN      
        1 ggatttaata atatggataa aatgctataa taaaaacaga aagggatact aaaggagacg
       61 atgtaatgga cgataagatg tttgcactaa tagagaaaat gtatgttgaa atgcaaacga
      121 tgaaatctaa taaggaaact aaggaagaca tgtccggaat tacccaaaga ttaaccaaaa
      181 tcgatcaaag gttggccgtt atggaaaaca aaatggatac aaatcataag gccctatatg
      241 acggatacaa attgacctac gaaaaagcgg tcacccttga agagaaaatt gatgtaatag
      301 acaaaaaggt tgacaaacat gatgttgaga taagggttat taaagggata aagggataat
      361 ctatgtgttt ggtctgggtg ccaaaagggg acgttcctaa aatgtcacgc gaacgctagg
      421 gtaaattcta gagatcgttt ctattatcat caaagggatc aaaaacggat ggaagaaggc
      481 taacgatcca ttagcgacgg aaaatggcct tttataacaa tccgataaaa aggactcaag
      541 gctccattga tttaatcatt aaacttctct ataatcttca tataggcccc tctgcttggg
      601 ggctactcca tggaattatt catgatgaga atataggcaa agaatctaaa acaaatcgtc
      661 aaagggaatc cccctgtatt aaagaagatg ggtctagttg tttcaccaat attataaatt
      721 aaaggagaga gccatgaaga tcactgacgt aaaggctgcg aaaatcggag caaacattgt
      781 cataagggtg gtaacggaca aggatggcat ggacggttat agccagatag agagccataa
      841 agattatgtg atgcctttaa tcccctattt caagagcatg attgttggac atgatcccac
      901 gaatgtagaa agggtattgc gtcaaatgcg ccgttcggca gcgtttaagc cctggggcaa
      961 ggtctttagc tccatagaga tggccctatg ggatattgcg ggcaaggatg cgggcgttcc
     1021 ggtctataag cttttgggtg gaaaggttcg tgacagggtc agagtctata acggaagctt
     1081 cttagacccc aatcctccac atgaaatcgc cgatactccg gagaagcttg gcgaaaacat
     1141 tcttcaccta aacgagatac cggaaggctt tactattgtg aaggtcgccg ccttctggca
     1201 tatgccaaac ttttatggag aatccgatgc ctataatacc tactaccccc aaggccgggg
     1261 tgaggcaaat cgcctgggcg gttacacgaa ttacgtgaaa atgatcaacg aaggtaaaac
     1321 cctcagctcc aggttcaata tacctgttgt tcagggcagc ctgctcaccg agaagggcct
     1381 aaagaccagc atagaatatg tggaacggct gaaaaaggtt gtgggcgaca aggtcggcct
     1441 ggcctttgat atcgggccgg gatttatgcc cggagatgcg atccgcttgg caaaggcgct
     1501 ggagccgtac aacgttatgt ggcttgaaga ttccatagcc ggcgactaca caccttatac
     1561 catggcctct gtctacaggg acattaccac cagtacctct acgcccatac ataccgggga
     1621 gcagatattc ctgcgccaaa actttatgga acttattgaa acccgggcgg ttgatgttgt
     1681 aggacccgat cctgccgatg taggcggtat caaagaactc aagtggattg cagagtatgc
     1741 ggacctccat ggcattctga tggcacctca cggtaccttt aacggtgtat tcggtatggc
     1801 tgcccttacc catgtatgct ctacaatgcc gcaaaactac gtcgcctatg agtatccagg
     1861 cccaaatccg ggcttctggt tggatattgt tgaaggactg ccgaacaatt tccttcgcga
     1921 cggccatgta aaggtttggg atgctccggg tatcggcatt accttcaacg tcaacaaggc
     1981 ttctaagtac ctaagccccg aagacaagga cttcttcctg tgacatttgg ggaacgtccc
     2041 cttatgtcac ggagctggag atttcacccg ttaacaagtt aaaattaaaa aatcaaaaag
     2101 ggtcagaacc tcggcatcaa tgggggttcg gcccttttta aatatgttat acctagatat
     2161 catgatagca actaaaagcg cgaatcttgt ttcaaattaa tgtccaacca agcctttact
     2221 gatattataa ctaacacact cggttaaatt accccgctag aaaaatattg caccttagta
     2281 tacaataagg atacgatatc ggctaagggg atcgatgtta taacgagccg gtaagaagtc
     2341 tcccgcttct gtagggggcg agttcccatc agtaaaacag gcggtgggta taaattatcc
     2401 gcctcgttga aacctgtgtg ttgcaattgc caaggtcttg cttccgatgt tataacatag
     2461 aaaaggggtt atttatctat gcttgaagag aaaattaagc tggaaaccag gcttgtaggc
     2521 gatattgaag gaaagtttta tgtcccatca taccagaggg gatataggtg ggggaaaaat
     2581 gaggttacaa ggcttttaga ggacatatat tctaacggag aaaataatta ttgcctgcaa
     2641 cctatagtgg tcagggcaaa aggagaacat tacgaattaa tcgatggaca gcagaggtta
     2701 acgacaatat atttagtata caactacatg tttaggataa gcgggggctt tttgggccaa
     2761 ccgaaattta ctctggtcta tcaaacaagg gcagaaaccg aaaagttcct agaagacata
     2821 gacctgtcaa gaaaggaaga gaacatcgat ttctggttta taagcaacgc ctatgaaacc
     2881 atagaaagtt ggtttaaaga taaggagagg gtatc
//
